LOCUS       DAJTXY010000001       141769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTXY010000001 DAJTXY010000000
VERSION     DAJTXY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 141769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 141769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..141769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.001"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO566_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO566_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6603443.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO566_000002"
     CDS             complement(1672..2730)
                     /locus_tag="OO566_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6603444.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO566_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO566_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO566_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO566_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6603445.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO566_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO566_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6603446.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO566_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO566_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6603447.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO566_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO566_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6603448.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO566_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO566_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6603449.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO566_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO566_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6603450.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO566_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO566_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6603451.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFCCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO566_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO566_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6603452.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO566_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO566_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6603453.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO566_000013"
     CDS             complement(17699..18322)
                     /locus_tag="OO566_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6603454.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO566_000014"
     CDS             18619..18894
                     /locus_tag="OO566_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603455.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO566_000015"
     CDS             complement(18971..19213)
                     /locus_tag="OO566_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6603456.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO566_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO566_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6603457.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO566_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO566_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6603458.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO566_000018"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO566_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6603459.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKACVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22592..24289
                     /gene="ypdA"
                     /locus_tag="OO566_000019"
     CDS             22592..24289
                     /gene="ypdA"
                     /locus_tag="OO566_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6603460.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24304..25037
                     /gene="ypdB"
                     /locus_tag="OO566_000020"
                     /pseudo
     CDS             24304..25037
                     /gene="ypdB"
                     /locus_tag="OO566_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
     gene            25050..25907
                     /locus_tag="OO566_000021"
     CDS             25050..25907
                     /locus_tag="OO566_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6603461.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="OO566_000022"
     CDS             complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="OO566_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6603462.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDLSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="OO566_000023"
     CDS             complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="OO566_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6603463.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="OO566_000024"
                     /pseudo
     CDS             complement(29473..30556)
                     /gene="ypdF"
                     /locus_tag="OO566_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30571..31818)
                     /locus_tag="OO566_000025"
     CDS             complement(30571..31818)
                     /locus_tag="OO566_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6603464.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31840..32166)
                     /locus_tag="OO566_000026"
     CDS             complement(31840..32166)
                     /locus_tag="OO566_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6603465.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32385..33350)
                     /gene="glk"
                     /locus_tag="OO566_000027"
     CDS             complement(32385..33350)
                     /gene="glk"
                     /locus_tag="OO566_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6603466.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33554..34810
                     /locus_tag="OO566_000028"
     CDS             33554..34810
                     /locus_tag="OO566_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6603467.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34925..35251
                     /locus_tag="OO566_000029"
     CDS             34925..35251
                     /locus_tag="OO566_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6603468.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35392..36630)
                     /locus_tag="OO566_000030"
     CDS             complement(35392..36630)
                     /locus_tag="OO566_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6603469.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36966..38168
                     /gene="nupC"
                     /locus_tag="OO566_000031"
     CDS             36966..38168
                     /gene="nupC"
                     /locus_tag="OO566_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6603470.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38218..40407)
                     /locus_tag="OO566_000032"
                     /pseudo
     CDS             complement(38218..40407)
                     /locus_tag="OO566_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40615..40690)
                     /locus_tag="OO566_000033"
     tRNA            complement(40615..40690)
                     /locus_tag="OO566_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40655..40657),aa:Ala,seq:ggc)
     gene            complement(40730..40805)
                     /locus_tag="OO566_000034"
     tRNA            complement(40730..40805)
                     /locus_tag="OO566_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40770..40772),aa:Ala,seq:ggc)
     gene            41026..41385
                     /locus_tag="OO566_000035"
     CDS             41026..41385
                     /locus_tag="OO566_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6603471.1"
                     /translation="MSKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41387..41779
                     /locus_tag="OO566_000036"
     CDS             41387..41779
                     /locus_tag="OO566_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6603472.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41831..43246)
                     /gene="gltX"
                     /locus_tag="OO566_000037"
     CDS             complement(41831..43246)
                     /gene="gltX"
                     /locus_tag="OO566_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6603473.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43505..43580
                     /locus_tag="OO566_000038"
     tRNA            43505..43580
                     /locus_tag="OO566_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43538..43540,aa:Val,seq:tac)
     gene            43625..43700
                     /locus_tag="OO566_000039"
     tRNA            43625..43700
                     /locus_tag="OO566_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43658..43660,aa:Val,seq:tac)
     gene            43705..43780
                     /locus_tag="OO566_000040"
     tRNA            43705..43780
                     /locus_tag="OO566_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43738..43740,aa:Lys,seq:ttt)
     gene            43811..43936
                     /locus_tag="OO566_000041"
     ncRNA           43811..43936
                     /ncRNA_class="other"
                     /locus_tag="OO566_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            44056..44397
                     /locus_tag="OO566_000042"
     CDS             44056..44397
                     /locus_tag="OO566_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6603474.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44388..45314)
                     /locus_tag="OO566_000043"
     CDS             complement(44388..45314)
                     /locus_tag="OO566_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6603475.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45404..46402
                     /locus_tag="OO566_000044"
     CDS             45404..46402
                     /locus_tag="OO566_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6603476.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46399..46617)
                     /locus_tag="OO566_000045"
     CDS             complement(46399..46617)
                     /locus_tag="OO566_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6603477.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46619..48634)
                     /gene="ligA"
                     /locus_tag="OO566_000046"
     CDS             complement(46619..48634)
                     /gene="ligA"
                     /locus_tag="OO566_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6603478.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48705..49592)
                     /gene="zipA"
                     /locus_tag="OO566_000047"
     CDS             complement(48705..49592)
                     /gene="zipA"
                     /locus_tag="OO566_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6603479.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRSMMT
                     PQKLREYQDIIREVKDANA"
     gene            49822..50583
                     /gene="cysZ"
                     /locus_tag="OO566_000048"
     CDS             49822..50583
                     /gene="cysZ"
                     /locus_tag="OO566_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6603480.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50768..51739
                     /gene="cysK"
                     /locus_tag="OO566_000049"
     CDS             50768..51739
                     /gene="cysK"
                     /locus_tag="OO566_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6603481.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52123..52380
                     /gene="ptsH"
                     /locus_tag="OO566_000050"
     CDS             52123..52380
                     /gene="ptsH"
                     /locus_tag="OO566_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6603482.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52425..54152
                     /gene="ptsI"
                     /locus_tag="OO566_000051"
     CDS             52425..54152
                     /gene="ptsI"
                     /locus_tag="OO566_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6603483.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54193..54702
                     /gene="crr"
                     /locus_tag="OO566_000052"
     CDS             54193..54702
                     /gene="crr"
                     /locus_tag="OO566_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6603484.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54744..55595)
                     /gene="pdxK"
                     /locus_tag="OO566_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54744..55595)
                     /gene="pdxK"
                     /locus_tag="OO566_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6603485.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55700..56074
                     /locus_tag="OO566_000054"
     CDS             55700..56074
                     /locus_tag="OO566_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6603486.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            56107..56830
                     /locus_tag="OO566_000055"
                     /pseudo
     CDS             56107..56830
                     /locus_tag="OO566_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(57034..57945)
                     /gene="cysM"
                     /locus_tag="OO566_000056"
     CDS             complement(57034..57945)
                     /gene="cysM"
                     /locus_tag="OO566_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6603487.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MHAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(58079..59176)
                     /gene="cysA"
                     /locus_tag="OO566_000057"
     CDS             complement(58079..59176)
                     /gene="cysA"
                     /locus_tag="OO566_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6603488.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(59166..60041)
                     /gene="cysW"
                     /locus_tag="OO566_000058"
     CDS             complement(59166..60041)
                     /gene="cysW"
                     /locus_tag="OO566_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6603489.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(60041..60874)
                     /gene="cysT"
                     /locus_tag="OO566_000059"
     CDS             complement(60041..60874)
                     /gene="cysT"
                     /locus_tag="OO566_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6603490.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60874..61890)
                     /gene="cysP"
                     /locus_tag="OO566_000060"
     CDS             complement(60874..61890)
                     /gene="cysP"
                     /locus_tag="OO566_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6603491.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62194..62985)
                     /gene="ucpA"
                     /locus_tag="OO566_000061"
     CDS             complement(62194..62985)
                     /gene="ucpA"
                     /locus_tag="OO566_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6603492.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(63172..63972)
                     /gene="murR"
                     /locus_tag="OO566_000062"
     CDS             complement(63172..63972)
                     /gene="murR"
                     /locus_tag="OO566_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6603493.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            64136..65032
                     /gene="murQ"
                     /locus_tag="OO566_000063"
     CDS             64136..65032
                     /gene="murQ"
                     /locus_tag="OO566_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6603494.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            65036..66460
                     /gene="murP"
                     /locus_tag="OO566_000064"
     CDS             65036..66460
                     /gene="murP"
                     /locus_tag="OO566_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6603495.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66640..67539)
                     /gene="yfeX"
                     /locus_tag="OO566_000065"
     CDS             complement(66640..67539)
                     /gene="yfeX"
                     /locus_tag="OO566_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6603496.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(67635..68210)
                     /locus_tag="OO566_000066"
     CDS             complement(67635..68210)
                     /locus_tag="OO566_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6603497.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68271..68720)
                     /locus_tag="OO566_000067"
     CDS             complement(68271..68720)
                     /locus_tag="OO566_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6603498.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68707..69132)
                     /locus_tag="OO566_000068"
     CDS             complement(68707..69132)
                     /locus_tag="OO566_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6603499.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69346..70215
                     /gene="amiA"
                     /locus_tag="OO566_000069"
     CDS             69346..70215
                     /gene="amiA"
                     /locus_tag="OO566_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6603500.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70219..71118
                     /gene="hemF"
                     /locus_tag="OO566_000070"
     CDS             70219..71118
                     /gene="hemF"
                     /locus_tag="OO566_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6603501.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71052..72176)
                     /gene="eutR"
                     /locus_tag="OO566_000071"
     CDS             complement(71052..72176)
                     /gene="eutR"
                     /locus_tag="OO566_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR6603502.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITCQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSFG"
     gene            complement(72261..72722)
                     /gene="eutK"
                     /locus_tag="OO566_000072"
     CDS             complement(72261..72722)
                     /gene="eutK"
                     /locus_tag="OO566_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6603503.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(72735..73394)
                     /gene="eutL"
                     /locus_tag="OO566_000073"
     CDS             complement(72735..73394)
                     /gene="eutL"
                     /locus_tag="OO566_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6603504.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73404..74291)
                     /gene="eutC"
                     /locus_tag="OO566_000074"
     CDS             complement(73404..74291)
                     /gene="eutC"
                     /locus_tag="OO566_000074"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR6603505.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(74312..75673)
                     /gene="eutB"
                     /locus_tag="OO566_000075"
     CDS             complement(74312..75673)
                     /gene="eutB"
                     /locus_tag="OO566_000075"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCR6603506.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            75862..77067
                     /locus_tag="OO566_000076"
     CDS             75862..77067
                     /locus_tag="OO566_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6603507.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWINLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            77551..78321
                     /locus_tag="OO566_000077"
     CDS             77551..78321
                     /locus_tag="OO566_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6603508.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQEQEQEQEQE
                     QEQEQEQEQNKAAAIAEFKELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEE
                     AEEDLRFDSKEEQLPEEREQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELK
                     LYTWMVSKCRYRGNDKYEIWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALR
                     GKCGKTYRLRIPSKGTAEEASMTAQQHEREAKVSYLVH"
     gene            79372..80124
                     /locus_tag="OO566_000078"
     CDS             79372..80124
                     /locus_tag="OO566_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603509.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKQK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGYVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            80884..81279
                     /locus_tag="OO566_000079"
     CDS             80884..81279
                     /locus_tag="OO566_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6603510.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            81297..81518
                     /locus_tag="OO566_000080"
     CDS             81297..81518
                     /locus_tag="OO566_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603511.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLIC"
     gene            81583..82125
                     /locus_tag="OO566_000081"
     CDS             81583..82125
                     /locus_tag="OO566_000081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603512.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            82590..82796
                     /locus_tag="OO566_000082"
     CDS             82590..82796
                     /locus_tag="OO566_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603513.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            83108..83593
                     /locus_tag="OO566_000083"
     CDS             83108..83593
                     /locus_tag="OO566_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6603514.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            83574..83852
                     /locus_tag="OO566_000084"
     CDS             83574..83852
                     /locus_tag="OO566_000084"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603515.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            83845..85305
                     /locus_tag="OO566_000085"
     CDS             83845..85305
                     /locus_tag="OO566_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6603516.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            85339..86148
                     /gene="murI"
                     /locus_tag="OO566_000086"
     CDS             85339..86148
                     /gene="murI"
                     /locus_tag="OO566_000086"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6603517.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(86267..86958)
                     /locus_tag="OO566_000087"
     CDS             complement(join(86267..86712,86712..86958))
                     /locus_tag="OO566_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6603518.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALPPFNIGM
                     LTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEK
                     VIGAFIEKHMFY"
     gene            complement(87482..88855)
                     /gene="eutA"
                     /locus_tag="OO566_000088"
     CDS             complement(87482..88855)
                     /gene="eutA"
                     /locus_tag="OO566_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6603519.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(88830..89309)
                     /gene="eutP"
                     /locus_tag="OO566_000089"
     CDS             complement(88830..89309)
                     /gene="eutP"
                     /locus_tag="OO566_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6603520.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(89322..89657)
                     /gene="eutS"
                     /locus_tag="OO566_000090"
     CDS             complement(89322..89657)
                     /gene="eutS"
                     /locus_tag="OO566_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6603521.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(89950..92229)
                     /gene="maeB"
                     /locus_tag="OO566_000091"
     CDS             complement(89950..92229)
                     /gene="maeB"
                     /locus_tag="OO566_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6603522.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            92518..93468
                     /gene="tal"
                     /locus_tag="OO566_000092"
     CDS             92518..93468
                     /gene="tal"
                     /locus_tag="OO566_000092"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6603523.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            93488..95491
                     /gene="tkt"
                     /locus_tag="OO566_000093"
     CDS             93488..95491
                     /gene="tkt"
                     /locus_tag="OO566_000093"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6603524.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(95605..96636)
                     /locus_tag="OO566_000094"
     CDS             complement(95605..96636)
                     /locus_tag="OO566_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6603525.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQSFRLVRYAAEPTCDNW
                     HGPDARPTL"
     gene            complement(96762..97337)
                     /gene="nudK"
                     /locus_tag="OO566_000095"
     CDS             complement(96762..97337)
                     /gene="nudK"
                     /locus_tag="OO566_000095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6603526.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(97405..99384)
                     /gene="aegA"
                     /locus_tag="OO566_000096"
     CDS             complement(97405..99384)
                     /gene="aegA"
                     /locus_tag="OO566_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6603527.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDTPGVYDALPFIIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            99590..101313
                     /gene="narQ"
                     /locus_tag="OO566_000097"
                     /pseudo
     CDS             99590..101313
                     /gene="narQ"
                     /locus_tag="OO566_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            101515..104628
                     /gene="acrD"
                     /locus_tag="OO566_000098"
     CDS             101515..104628
                     /gene="acrD"
                     /locus_tag="OO566_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6603528.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNAAINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKAEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(104727..104786)
                     /gene="ypfM"
                     /locus_tag="OO566_000099"
     CDS             complement(104727..104786)
                     /gene="ypfM"
                     /locus_tag="OO566_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6603529.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            105167..105523
                     /locus_tag="OO566_000100"
     CDS             105167..105523
                     /locus_tag="OO566_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6603530.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            105527..106654
                     /gene="dapE"
                     /locus_tag="OO566_000101"
     CDS             105527..106654
                     /gene="dapE"
                     /locus_tag="OO566_000101"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6603531.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIIEQLVA"
     gene            106682..106882
                     /locus_tag="OO566_000102"
     CDS             106682..106882
                     /locus_tag="OO566_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6603532.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(106992..107690)
                     /gene="ypfH"
                     /locus_tag="OO566_000103"
     CDS             complement(106992..107690)
                     /gene="ypfH"
                     /locus_tag="OO566_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6603533.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(107764..109779)
                     /gene="tmcA"
                     /locus_tag="OO566_000104"
     CDS             complement(107764..109779)
                     /gene="tmcA"
                     /locus_tag="OO566_000104"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6603534.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAL
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGCLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(109794..110657)
                     /locus_tag="OO566_000105"
     CDS             complement(109794..110657)
                     /locus_tag="OO566_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6603535.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(110825..111538)
                     /gene="purC"
                     /locus_tag="OO566_000106"
     CDS             complement(110825..111538)
                     /gene="purC"
                     /locus_tag="OO566_000106"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6603536.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(111751..112785)
                     /gene="bamC"
                     /locus_tag="OO566_000107"
     CDS             complement(111751..112785)
                     /gene="bamC"
                     /locus_tag="OO566_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6603537.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(112802..113680)
                     /gene="dapA"
                     /locus_tag="OO566_000108"
     CDS             complement(112802..113680)
                     /gene="dapA"
                     /locus_tag="OO566_000108"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6603538.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            113826..114398
                     /locus_tag="OO566_000109"
     CDS             113826..114398
                     /locus_tag="OO566_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6603539.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            114398..114868
                     /gene="bcp"
                     /locus_tag="OO566_000110"
     CDS             114398..114868
                     /gene="bcp"
                     /locus_tag="OO566_000110"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6603540.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            115121..115738
                     /gene="hyfA"
                     /locus_tag="OO566_000111"
     CDS             115121..115738
                     /gene="hyfA"
                     /locus_tag="OO566_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6603541.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            115738..117755
                     /gene="hyfB"
                     /locus_tag="OO566_000112"
                     /pseudo
     CDS             115738..117755
                     /gene="hyfB"
                     /locus_tag="OO566_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            117766..118707
                     /gene="hyfC"
                     /locus_tag="OO566_000113"
     CDS             117766..118707
                     /gene="hyfC"
                     /locus_tag="OO566_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6603542.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGLASALLRLHGSSG"
     gene            118735..120174
                     /gene="hyfD"
                     /locus_tag="OO566_000114"
     CDS             118735..120174
                     /gene="hyfD"
                     /locus_tag="OO566_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6603543.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            120186..120836
                     /gene="hyfE"
                     /locus_tag="OO566_000115"
     CDS             120186..120836
                     /gene="hyfE"
                     /locus_tag="OO566_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6603544.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            120841..122421
                     /gene="hyfF"
                     /locus_tag="OO566_000116"
     CDS             120841..122421
                     /gene="hyfF"
                     /locus_tag="OO566_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6603545.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFFFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            122411..124125
                     /gene="hyfG"
                     /locus_tag="OO566_000117"
                     /pseudo
     CDS             122411..124125
                     /gene="hyfG"
                     /locus_tag="OO566_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            124135..124680
                     /gene="hyfH"
                     /locus_tag="OO566_000118"
     CDS             124135..124680
                     /gene="hyfH"
                     /locus_tag="OO566_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6603546.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            124677..125435
                     /gene="hyfI"
                     /locus_tag="OO566_000119"
     CDS             124677..125435
                     /gene="hyfI"
                     /locus_tag="OO566_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6603547.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            125428..125841
                     /gene="hyfJ"
                     /locus_tag="OO566_000120"
     CDS             125428..125841
                     /gene="hyfJ"
                     /locus_tag="OO566_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6603548.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            125871..127883
                     /gene="hyfR"
                     /locus_tag="OO566_000121"
                     /pseudo
     CDS             125871..127883
                     /gene="hyfR"
                     /locus_tag="OO566_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            127905..128753
                     /locus_tag="OO566_000122"
                     /pseudo
     CDS             127905..128753
                     /locus_tag="OO566_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(128791..129852)
                     /locus_tag="OO566_000123"
     CDS             complement(128791..129852)
                     /locus_tag="OO566_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6603549.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            130065..131528
                     /gene="bepA"
                     /locus_tag="OO566_000124"
     CDS             130065..131528
                     /gene="bepA"
                     /locus_tag="OO566_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6603550.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSNLLDEWAKGNVLQQRAAQYGRALQAMKANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            131549..131908
                     /gene="arsC"
                     /locus_tag="OO566_000125"
     CDS             131549..131908
                     /gene="arsC"
                     /locus_tag="OO566_000125"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6603551.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(132041..132787)
                     /gene="hda"
                     /locus_tag="OO566_000126"
     CDS             complement(132041..132787)
                     /gene="hda"
                     /locus_tag="OO566_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6603552.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(132837..134126)
                     /gene="uraA"
                     /locus_tag="OO566_000127"
     CDS             complement(132837..134126)
                     /gene="uraA"
                     /locus_tag="OO566_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6603553.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(134212..134838)
                     /gene="upp"
                     /locus_tag="OO566_000128"
     CDS             complement(134212..134838)
                     /gene="upp"
                     /locus_tag="OO566_000128"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6603554.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            135163..136200
                     /gene="purM"
                     /locus_tag="OO566_000129"
     CDS             135163..136200
                     /gene="purM"
                     /locus_tag="OO566_000129"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6603555.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            136200..136838
                     /gene="purN"
                     /locus_tag="OO566_000130"
     CDS             136200..136838
                     /gene="purN"
                     /locus_tag="OO566_000130"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6603556.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            137010..139076
                     /gene="ppk1"
                     /locus_tag="OO566_000131"
     CDS             137010..139076
                     /gene="ppk1"
                     /locus_tag="OO566_000131"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6603557.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            139081..140622
                     /gene="ppx"
                     /locus_tag="OO566_000132"
     CDS             139081..140622
                     /gene="ppx"
                     /locus_tag="OO566_000132"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6603558.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPSTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(140661..>141769)
                     /gene="pdeF"
                     /locus_tag="OO566_000133"
     CDS             complement(140661..>141769)
                     /gene="pdeF"
                     /locus_tag="OO566_000133"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6603559.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        35403 a        36531 c        34913 g        34922 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgttgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagtggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaacaaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggtag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccctc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aatgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata ccccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ccgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgag atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggtgtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaagc cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgcccct gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacaga aaatttttct
    22381 acgctaacaa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac
    23041 gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    23101 ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct
    23161 ggtgcaaagc gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct
    23221 ggatatcgcc aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa
    23281 ggtctgcgaa attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga
    23341 tcatgtgctg gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat
    23401 tagcccgact acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga
    23461 agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggggt
    23521 cgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca
    23581 ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga
    23641 gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc
    23701 ccattttctg tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac
    23761 cgctcgccag ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga
    23821 cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga
    23881 gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag aggtgaattg
    23941 ctgcattccc agcctgctga tccagccgtt ggtggagaac gccattgtcc acggtattca
    24001 gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggaa atcgggtacg
    24061 cattgcggtg cgagataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc
    24121 gaatgaaatg ccgggcaata aaattggcct gctgaatgtc catcatcgcg tgaagttatt
    24181 gtatggcgag gggctgcata tccgccgcct ggagccgggg acggaaattg cgttttacat
    24241 tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat
    24301 attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta
    24361 attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg
    24421 aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg
    24481 gatggcgtgt tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc
    24541 atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt
    24601 ctcaaaccat atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg
    24661 caacaacagc agaccagcgt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    24721 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    24781 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    24841 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    24901 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    24961 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    25021 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    25081 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    25141 ggtttgccag ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc
    25201 tggatatcgt gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg
    25261 tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac
    25321 cggtgatgct gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata
    25381 gccgcaccac gtcgttgttg catcctgctc gccagatcca gcttcccagc ctgcaacgcg
    25441 gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga
    25501 atatcattca gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc
    25561 cgccgggtaa ttcgcaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc
    25621 aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc
    25681 ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc
    25741 gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg
    25801 aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc
    25861 agtttggtct gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta
    25921 attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc
    25981 ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc
    26041 cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc
    26101 agtcaaccgg tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt
    26161 tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc
    26221 agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc
    26281 acaggaactg gatcgcctgc tctttattgc taagatcctg atcaacaaag atattctcca
    26341 gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt
    26401 cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca
    26461 gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca
    26521 ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc
    26581 ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg
    26641 atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca
    26701 gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt
    26761 cgatgatgta gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat
    26821 gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata
    26881 agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg
    26941 cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta
    27001 ccgcgcgata gccgaggaat gggttctctt cctggggaac attcagataa gaaatgcttt
    27061 tatcgccgcc gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc gccagtagca
    27121 cctgctggta ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga tccatataga
    27181 gcatttcggt acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt
    27241 ccagagcggt gccgatattc gccgcaatat caatacgctt gttatcacgg gaataggcca
    27301 gctgcgcggc agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct
    27361 gataataacc gctcaccgcg tcattcgggt taatcgccag cacgccgcac tgggcgtcaa
    27421 gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaagcca ctcagtaccg
    27481 ggatcgccga ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa
    27541 tcatacccgc aagatttttc aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag
    27601 ccaccagaat ggtcggtttt tccagcacca gattgttgcg cggcttcagt tccggccagg
    27661 tgatatgcag caactgttcg ctgatatcgc gaatgtcgct gacccgctcg cgcagataat
    27721 cgctggcaga agcagatagt ttggcgcaaa cctgctccat attgctgatg atcgccgccc
    27781 ccagtccctg atgctgttct gtcatcaggc gacggatatt gcctgcaaat tcatcatcct
    27841 gaatcagcga caaatgagcg ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt
    27901 gctgattcag ttgctcggca agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg
    27961 cactggcggg gattgcccga taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac
    28021 ccacgccgac gccgcttgcc agcacattgc cgtacagtaa atccgggtta aggcggctta
    28081 atgaacgcgg cagcggatgc gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt
    28141 cgataaagcg caccaggatg tactcttcca gcacgcgccg cgcctgctct tcatcgctgc
    28201 cgctaatgtt caggctgcaa ctgtcattaa ataaggtgcc agttccaatc aacgccagcg
    28261 agcttttagc atcggccttt gcgttctggc gatggttgat aaaggtgatt tcgctttgcc
    28321 actggctgca ctgttcttta agttcccgcg ccggacgagc atgtagaccg ttaggcatag
    28381 gcaaaggaca gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc
    28441 tgaaatccgt cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa
    28501 gttgctccat ctgcaaaatg tcgcggcaat cggcaatcga tgccgcgcaa tgtccgtggc
    28561 gggtggcagg ccccattacc aatgtgggta cgccagtacc ggttaagtgc accgctccac
    28621 cgtccgtgcc gccgttactg aacatatccg cctgcaacgg aacgccaatt tctgccgcca
    28681 cggtttcaat ccacgcggta agttttggcg gcgcaatcag cgatttatcg cttaacacca
    28741 gcatcggccc gttaccaatc tgacgatggt tagccgcgcc ataatcaaag tttttcgccc
    28801 agcaggcggt atcaagcaca atggcgacgt ccggcgacac cgcgcgggta gcagtttgcc
    28861 cgccgcgtaa tcccacctct tcgctggaac ttgccaccag ccacacttcc gcaggcagtt
    28921 cggcgtcgtg cagctcacgt agtaacgtca ccagcagata gcaaccgagg cgatcatcaa
    28981 aggctttgcc catcacccgc tggtgaggga ggacctgaaa agtggtatcg aaggtgacgc
    29041 gatcgcctgg acgaattccc gcctgcatca cttcgtcata ggtgcgcgcg ccaatgtcca
    29101 cgcgcatggc gctaacgtca ttcccctgcc ggtcgccgtc aagcaggcct agaattttgc
    29161 actcttcacg ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgcacgttgc
    29221 caaccggcag cacgtcaata gcgccttcgc gggagatgct gcgcaccata aatcccactt
    29281 cgtccatatg ggcgcagatc atcaccttcg gacctgtcga ttcattgagg cggatcagca
    29341 ccgatcccag accatcaaat cgcacttctt tttgcaggcg agccgcttct tccagcagga
    29401 tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gcttttaata
    29461 gcgataaatc cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac
    29521 ttctgcgcct tgcggggtaa ccagcacaac atcttcgatg cgcacgcccc cttgccctag
    29581 caaataaatc cccggctcca cggtcagcaa catgcctggc tgtagcgtcg tggtgtcccg
    29641 cggtgaaaaa cgcggatctt catggacttc aatgccgata gcgtgaccag tgttatgacc
    29701 gaaatagtcg ccaaatccag actcggtaat gacccggcgc gcggcgtcgt caatctgctg
    29761 gcagcgcacg ccggggcgaa ttgcagagat tgccgcgagc tgtgcctgca ggacaatctg
    29821 atagacgtca aacagcgggt gagattcggc gctcacccct tcgccattca ccagcaaggt
    29881 gcgcgtcata tcagcagtag ccctgataca gcgcaccaaa atcgagagtg acaaactcgc
    29941 ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccgcgc cagccgctgg
    30001 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
    30061 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
    30121 cgcaatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
    30181 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
    30241 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
    30301 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
    30361 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
    30421 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
    30481 tctgccgtga ggagagaagc accgcatcca gttgttgcac cttaagccag ttgcgcagcg
    30541 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttgccttt
    30601 gcggaacatc atcagacgca ggaacaccac catcagcgca gtaatgattg cccccagcgc
    30661 gatccccgcc atataaacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
    30721 cgggaaccat tgcactgcgc ccagccagac agctgcggtt gagccgacaa tcgcgccgac
    30781 catatatgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
    30841 aatccccata aaggccagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
    30901 caggcgtttg ccggttaaac gacggtcaat aatggtcgcc agacccagac caatcggcgg
    30961 aataacgata gcgatagaac gagcggtgac cggtaatacg tggtcagtgg taaagctgaa
    31021 ggcaacgaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgctg tcgccgcggc
    31081 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cagtacggat
    31141 accgccgttg atccagccac caaagggggt gatgacgtag tacatcgcca gcatgacaaa
    31201 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
    31261 ctggattttc tggttcatcc atttcacaag gtaaccggca acgatagaaa taatcagcgc
    31321 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
    31381 gttcagcagt tgcgtcggct gggtagacat caacccgccg ataaaaccag ccgggaacgc
    31441 cagtttgcca ccgatagagt tcgccacaaa tgcagcaaac atcggaatgg caaaaccaaa
    31501 caacacgccg ccgaaggact gcgacagcca ggcaaattta agtaaggaga ggtcgaaacc
    31561 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
    31621 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
    31681 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
    31741 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
    31801 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
    31861 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
    31921 ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc ggggtaactg ccacggaatg
    31981 gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg cgattctgga tgccgtccgc
    32041 gccctgggtt tcaattttca cttcataacc ggcttctacc gccgcttctt ccagcgcctg
    32101 agcggccata aaagtgtgag ccaggcccat tgggcaggca cataaggcaa tcagtttctt
    32161 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
    32221 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
    32281 tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa ttgaattaac tgtatgattt
    32341 aaaatattat cgggagagtt acctcccgat ataacaggaa ggatttacag aatgtgacct
    32401 aaggtctggc gtaaatgtgc accggaaccg agaagacccg gatagtcatg gacgatgaga
    32461 tacaccggaa tatcatggac atattcttta aagcgccctt tatcttcaaa tgcggcacgg
    32521 aaaccggagg ctttgaagaa ctcaaggaag cgcggcacga taccgcccgc aataaacacg
    32581 ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa aacggcccat aatgacgcaa
    32641 aacagcgaca atgcgcggcg gcaatcggtg cagctgtcag ccagcgcgcg ttcggtaata
    32701 tcttttggct tgagattttc tggcaggcgg ttgtcagctt tcacaattgc gcgatacaaa
    32761 ttcaccagcc cagggccaga aagcacgcgc tccgccgaaa catgaccaat ttccgcacgc
    32821 aatatttcga ggataatggc ctcttcttca ctattcggcg caaaatcaac gtgaccgcct
    32881 tcgcccggca agcttaccca acgcttatcg acatggacca gatgcgcaac cccaagcccc
    32941 gttccggcac cgtaaaccgc aataggctta ccttcgaccg gttctgcgcc accaaactga
    33001 atcagatgct cttttttcag catcgggatc gccatcgata cagcggtaaa atcgttaata
    33061 atttccagat ggctaaaacc gagattcttt ttcatttcgg caattgagaa cgcccaggta
    33121 tggttggtca ttgccaccca gtcaccggta attgggcaag cgatggcaat acagccgtct
    33181 ttcacctcga ccttatgttc ttcaagataa acgcgaatga ccgcttcgag gctggggtaa
    33241 tcaagccctg aataggtctt agcctgcgag atttcaccac tggcaatatc acacagagca
    33301 agacgtgcgt tggtgccgcc cacatcaccg actaatgcat actttgtcat tcttcaactg
    33361 ctccgctaaa gtcaaaataa ttctttctca cactgtaaat acttgggggc ataacaacaa
    33421 cgccggaaag gcaggctccc tgtaaatatc gatctgggtc acacaattac tttatcgttt
    33481 cagcaccaat tgcagcgatg cctttttgca agctgggcaa actaagtatc tgaccccgca
    33541 taaggaatag aacatgctcc atccgcgagc cagaaccatg ttgttattat cgctccccgc
    33601 cgtggcaatt gggattgcgt ccagtcttat tctgattgtg gtgatgaaaa tcgcctcggt
    33661 attacagaat ttgctctggc aacgactgcc gggaactctg gggatagccc aggattcccc
    33721 cctctggatc atcggtgtat taacgctaac gggtattgcg gtggggttgg ttatccgttt
    33781 cagccagggt catgccggac cagaccccgc ctgtgaaccg ctgatcggcg caccggttcc
    33841 gccctctgcg ctacctggac ttatcgtagc attaattctc ggtcttgctg gcggcgtcag
    33901 cctggggccg gaacatccga tcatgaccgt caatatcgcc cttgccgtcg ccattggcgc
    33961 tcgtctgtta ccgcgcgtca accgaatgga gtggactatt ttagcctctg ccggaaccat
    34021 cggcgcgctg tttggtacac ctgttgcggc ggcgttgata ttttcgcaaa ccttaaatgg
    34081 cagtagtgaa gttccgctat gggatcgtct ttttgcgccg ttaatggcgg cagcagctgg
    34141 tgcacttact accggattat ttttccatcc ccatttttca ctgcccattg cgcattacgg
    34201 acagatggaa atgaccgata ttctcagcgg tgcaattgtc gcggcgattg ccatcgcagc
    34261 agggatggtc gccgtatggt gcttaccacg gttgcacgcg ataatgaatc aaatgaaaaa
    34321 tccggtgctc gtgctgggta ttggcggatt tattctcgga attctggggg ttattggtgg
    34381 accagtttcg ctgtttaaag ggctggatga gatgcagcag atggtggcaa atcaggcttt
    34441 cagcaccagc gattactttt tgctggcggt aattaaactt gccgccctgg tcgttgctgc
    34501 cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg tttgtcggcg tggcattagg
    34561 gttgatgctg catgagcacg ttcccgccgt tccagcggca ataaccgttt cctgcgctat
    34621 tctcggcatc gtgctggtgg taacacgcga tggctggtta agtcttttta tggcggcagt
    34681 cgttgtaccc aataccacat tgctaccgct gctctgtatc gtcatgcttc cggcatggct
    34741 gttattagca ggtaagccga tgatgatggt caatcgtccg aagcaacagc caccccacga
    34801 taatgtttag caaaaaatgc tctcctttat gatttaagag ttatggcgat gatacggggc
    34861 tttacctccc cgtaatattg cgttaacagg ccgctgacgg cttcgatcgt gaaggagaat
    34921 aacaatgttc aggtcactgt ttctggcggc cgccctgatg gcatttaccc cgcttgcagc
    34981 aaatgcaggt gaaatcaccc tactgccatc gatcaaatta caaattggcg atcgcgatca
    35041 ttacggtaat tactgggacg gtggtcactg gcgagatcgt gattactggc atcgcaatta
    35101 tgaatggcgc aaaaaccgct ggtggcgtca tgataatggc tatcatcgtg gctgggataa
    35161 gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc gatcgtgacg atcatcgcgg
    35221 aaaaggccgc ggacatgggc accgccatta aacgcgtcct ccaatggagc acaatgcctg
    35281 atgcgacgct agcgcgtctt atcaggccta caaaccggct ctttaacgcg tagggcggat
    35341 aaggcgttta cgccgcatcc gccaatagtg ccagatacga cgctcattca actacaatcc
    35401 cagcgccgtc cccaccaaca accagatatt cagcgccacg accagcacta caatcaccca
    35461 gcctgtctgt tttacgcgtt tgctgttcac cagatcgccc atcaacttgc tgtcactggt
    35521 gaaaatcagc agtggaacca gcgccagggc gataccaaaa cttaacagca cctgactcat
    35581 aaccagaatc cgtgtcggat ctaatcccat cagaatgaca ataaatgacg gcaacatggt
    35641 gactgtacga cgcacccaca gcgggatatg gaagcgaatg aatccctgca tcaccacctg
    35701 ccccgccagt gtccccacca ccgttgagga cagtccggca gcaaccagac ttaacccaaa
    35761 gaccgttgcc gcagcatggc ttaacagcgg ttgcagcgtc agataagcct catcaagatc
    35821 ggcaacacca gtatgaccag aaaagtggaa cgccgccgca gctgtagcca tcatcgccag
    35881 attgacaaaa ccggcaatcg tcatggcgat agccacatcc catttggtgg cggaataacg
    35941 ttgttgacgc gaaccgccat gtaaatgctg agtgagcgaa gagtgcaaat aaatcacatg
    36001 cggcataatc gtcgccccta acacgcctgc tgccaggaag accgcctccg aagtaggtaa
    36061 actcgggatc accattcctt tacccagctg cgccaggtta ggctgggaga aaatcaactc
    36121 gacaatgtaa gccgcggcaa caaacaacag taacccgcca atcactttct ccagcggttt
    36181 ttgcccgcga cgttgcagca ttaaaatcag gaaagtcgcg atccccgtca gcaccgcgcc
    36241 ctgcaacaac gaaacaccaa gaatgagttt aaaaccgatc gccgcaccaa taaattccgc
    36301 caggtcggtt gccatcgcaa taatttctgc ctgaacccaa tagaaccaca ctacgggacg
    36361 cggatagtga tcacgaatct gctccgccag atttttaccg gtggcaatcc ctagtttggc
    36421 agagaggatc tgaattagca tcgccatcag gttggcccaa acgacaaccc acagtagctg
    36481 atagccgaag ctagcacccg cctgaatatt ggtcgcaaag ttaccgggat cgatataacc
    36541 aatcgccgca atgaacgcag gtcccattaa tgcgagcctc atcttgcgcg ccgcccgtcc
    36601 gctgctactc tcaacgcgat agttcgtcat cttgtgcctc taaaacatag cctttgctat
    36661 gtttcatgct attctaaacg agaatgatta tcaaattcat ttaaatggat tgtggtgatt
    36721 tctctgatag accaagatta cgattacgaa aagattgatg acttgaatgt tatggttatg
    36781 tttaatgtta gcacatttac ataactttca gcttccatac acaacatagc agaaatgtat
    36841 gacagatcac tatttttgaa gcctgtcaca ggacgtcatt atagtgtgtg tcagatctcg
    36901 ttttccttaa ccatgttaca tagaatgtgc acggaaattt aacctgcctc atatttggag
    36961 caaatatgga ccgcgtcctt cattttgtac tggcacttgc cgttgttgcg attctcgcac
    37021 tgctggtaag cagcgaccgc aaaaaaattc gtatccgtta tgttattcaa ctgcttgtta
    37081 tcgaagtgtt actggcgtgg ttcttcctga actccgacgt tggtttaggc ttcgtgaaag
    37141 gcttctccga aatgttcgaa aaactactcg gatttgccaa cgaagggact aacttcgtct
    37201 ttggtagcat gaatgatcaa ggcctggcat tcttcttcct gaaagtgctg tgcccaatcg
    37261 tctttatctc tgcactgatc ggtattctcc agcacattcg cgtgttgccg gtgattatcc
    37321 gcgcaattgg tttcctgctc tccaaagtca acggtatggg caaactggaa tcctttaacg
    37381 ccgtcagctc cctgattctg ggtcagtcag aaaactttat tgcctataaa gatatcctcg
    37441 gcaaaatctc ccgtaatcgt atgtacacca tggctgccac ggcaatgtcc accgtgtcga
    37501 tgtccatcgt tggtgcatac atgaccatgc tggaaccgaa atacgtcgtt gctgcgctgg
    37561 tactgaacat gttcagcacc tttatcgtgc tgtcgctgat caatccttac cgtgttgatg
    37621 ccagtgaaga aaacatccag atgtccaacc tgcacgaagg tcagagcttc ttcgaaatgc
    37681 tgggtgaata cattctggca ggtttcaaag ttgccattat cgttgccgcg atgctgatcg
    37741 gctttatcgc cctgatcgcc gcgctgaacg cactgtttgc caccgttact ggctggtttg
    37801 gctacagcat ctccttccag ggcatcctgg gctacatctt ctatccgatt gcatgggtga
    37861 tgggtgttcc ttccagtgaa gcactgcaag tgggcagtat catggcgacc aaactggttt
    37921 ccaacgagtt cgttgcgatg atggatctgc agaaaattgc ttccacgctc tctccgcgtg
    37981 ctgaaggcat catctctgtg ttcctggttt ccttcgctaa cttctcttca atcgggatta
    38041 tcgcaggtgc agttaaaggc ctgaatgaag agcaaggtaa cgtggtttct cgcttcggtc
    38101 tgaaactggt ttacggctct accctggtga gtgtgctgtc tgcgtcaatc gcagcactgg
    38161 tgctgtaaaa ccatacataa aaaagccggg gatcattccc cggctttttt acgcctgtta
    38221 atcagctaat ggctgcgggc gaccaatcaa atacccttgc agatattgca ccccgagctt
    38281 atgcaatagc gcctgctgct gttgcgtctc gacaaactcc gcgaccacac tcaatgactt
    38341 cgctttcgcc agatcggtaa ttgatctcac aatcatcgca tccagcgtat tcgtgacaat
    38401 atctttcaca aagacgccat caattttgat gatatcagcc tgcaaacgct ttaaccgttc
    38461 gtagttggca tatccggtgc caaagtcatc aatcgcaatc cggaaaccaa acttatgcag
    38521 ctgctcgatg ttgtacatgc tgctttctgc gttagaaaac gcctgctcct cggtgatctc
    38581 aagaatgacc gcctgcgggg agatgtgata acgcttaaac agacgaataa tccgtccggc
    38641 aatattcttt tgcagcagcg tgagcggcat taaattgact gaaaagcgcg gtcctttttt
    38701 gtcgcaaggg tgtgttgcca gccacttcaa caaggattcc agcacttgca aatcaaaacg
    38761 cgcgctaagg ttaaactgag caataagggg cagaaactta tccggggtca taatgccgcc
    38821 gtcatatttc agtcgcgcga ggatctcatc ataaccttca ccctctttgt tgcgaattgg
    38881 ctgggcgtag agcagcaaat caccctgatc taacgccgtg cgaatggtat tcagcaatag
    38941 cacctgttta gtggtctgcc cggaaaccat ctcctctctg ctatccagcg ccagcacatg
    39001 atgatgtgcg caggattgct ccgccagcca gcttaactgc cccaacaagg gttgcagggt
    39061 ttcctgattt ccatcaaaac gcccccaggc agcaccatag cccatatcca gcccggtatt
    39121 gttccagtga atttgccgac tattcaggat gttaaccata tgctggagtc gcccttccgt
    39181 ttccggcccg ctcagcacta acagcagttc actacccggc aattgataca acttttcgtt
    39241 ttcctgcatc aacggcagca gcgtacggca aattgagcgg atacagtgaa cgcgcatcat
    39301 taagccgtaa tgacgactca taaactcaag attatcaatg cgcaggcagc aaaaactctt
    39361 gcccgcctct tgctccggcg cttgttccaa cgcacgaaag ttgggtagga gcgttaacgg
    39421 atctgttagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    39481 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcgagaataa atgccagcga
    39541 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    39601 aacagcctag gtgagattta aaaacggata gcgaagatta ccgaccccga gggtaaagat
    39661 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    39721 cagaagtagc agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    39781 gcccaacgac ggagcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    39841 catgcgagtg agatagtaga aaagcatgtt gtaaatcagc actgcggtga aaaggcttag
    39901 taaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    39961 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    40021 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    40081 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctgccgag
    40141 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    40201 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    40261 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    40321 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    40381 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    40441 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca ggggccagcc tgttgccgat
    40501 ttttcatcca atgatgcttt caccaggcgg cgctaacccg gacgtaaact gtattacaaa
    40561 agcggcaaaa agcagagaca aaaaaccccc gctttgcagc gagggttgga aatttggtgg
    40621 agctaagcgg gatcgaaccg ctgacctctt gcatgccatg caagcgctct cccagctgag
    40681 ctatagcccc acgatgcgtt tacgtaccaa atttgctggg tgcaaaattt ggtggagcta
    40741 agcgggatcg aaccgctgac ctcttgcatg ccatgcaagc gctctcccag ctgagctata
    40801 gccccgtcac gtaaagcttg tcgagttgac gggcggcatc atatgaattc cgcccgaatg
    40861 tgtcaacggc aaattgcaac gtgtagtttc aatcgctgaa aaatcaggca aatgaacaat
    40921 tttggaaaac cgctcgcatt cagtagttat tcatgtcacg gtttcctgta aagtggtgtt
    40981 ataaaatgaa ctactaatag acccacatac attcagggaa ttgttatgtc caaggagagg
    41041 atgacaccag atgaacttgc cagactgacc ggctatagcc gccagaccat taataaatgg
    41101 gtacgcaagg aaggctggac aacgtcacca aaacctggcg tccagggtgg taaagcccga
    41161 ctggttcacg tcaatgaaca ggttcgtgaa tatattcgca atgctgaacg tccagaaggt
    41221 cagggagaag cgcctgccct ttctggtgat gcgccgcttg aagtattgct ggtgacactg
    41281 gcgaaagaga tgacgccagt tgaacaaaaa cagtttacat ccttgcttct gcgggaaggg
    41341 attatcggat tgttacaacg cttagggatt cgcgatagca aataatatga aaagattacg
    41401 caataaaatg accaccgaag aactggctga atgcctcggt gtggctaaac aaactgttaa
    41461 ccgttggatc agagaaaaag gctggaaaac ggaaaaattt cccggcgtga aaggcggtcg
    41521 tgccagactt attctggtcg atacgcaagt ttgcgagttt attcagaaca cgccagcctt
    41581 ccataacaca ccaatgttga tggaagctga agagagaatt gctgaatacg ctcctggagc
    41641 acgcgccccg gcgtatcgac aaatcatcaa cgcgatagat aatatgacag acattgagca
    41701 ggaaaaagtc gcgcaatttt tgtcacgtga aggaattcgc aatttcctcg cccgtctcga
    41761 tattgacgaa tcagcataaa taaaaaacgg caggatatta tctcctgccg tttatctttt
    41821 tacacgctaa ttactgctga ttttcgcgtt cagcaataaa atccagcgct ttgttgatac
    41881 gctcgatact gcgggtctta ccaattgcgt gaacggtaac atccagtgct ggagactgcc
    41941 ccgcaccggt tacggcgaca cgcagcggca taccaacttt acccataccc acttccagct
    42001 catccgccgt cgcctgaata gcgtgatgaa cgttttcagc ggtccagtca gtaatcgcgg
    42061 ccagtttgtc acgaaccact tccagcggct gacgcgctac cggacgcaga tgttttttcg
    42121 ccgcatcggc atcgaactca gcaaaatctt cgtagaaata acggcagctc tgtgccatct
    42181 ctttcagcgt cttgcagcgt tcgcccagca gtttcaccag atcagccagc tgcgggccgt
    42241 tacgggtatc gatattttcc tgctcaatgt gccactgtaa gtgagtagca acatactccg
    42301 gcggcagcgc gttaatgtag tgatggttca gccacagcag cttgtcggtg ttgaacgcac
    42361 tggcagattt gctgacggca ttcaaagtga agtatttgat catctcttca cgagtgaaga
    42421 tttcctgatc gccgtgggac cagcccagac gcaccagata gttcagcagt gcttctggca
    42481 aataaccgtc atcacgatac tgcattacgc tgactgcccc gtgacgtttg gacagttttt
    42541 taccgtcatc gccattgatc atagaaacgt gcgcgtaaac cggcaccggc gctttcaggg
    42601 ccttaagaat gttgatctgg cgcggcgtgt tgttgatatg gtcttcgcca cggataacgt
    42661 gggtgatttc catatcccag tcatcgacaa ccacacagaa gttataggtt ggggaaccat
    42721 cggtacggcg gataataaga tcgtccagtt cctggttgct gaactcgatc ggaccacgga
    42781 tctgatcgtc aaaaacaaca gaaccttcct gcgggttagc aaaacgtaca acacacggtt
    42841 catcatcagc atgatgctca tggctgtggc ggcagcgacc gtcataacgc ggcttctcac
    42901 ctttcgccat ttgctcttcg cgcagcgctt ccaggcgctc tttagagcaa tagcatttat
    42961 aagcagtgcc ctcttccagc atctgatcga tcaccgcgtt gtagcgatca aaacgtttgg
    43021 tctggtagta cggaccttca tcccactcca ggctcagcca gttcatgcca tccataatgg
    43081 cttcgatagc ttccggcgtg gaacgctcaa gatcggtgtc ttcaatacgc agcacgaact
    43141 caccgccgtg gttacgtgca aaaagccagg agtaaagagc agtacgcgcg ccgccaacgt
    43201 gcagatagcc tgttgggctt ggcgcgaagc gagttttgat tttcatgaaa tggccttacg
    43261 tttagaaaga tgccgacaac cggcaaatcc tggaaaaatt gagtgggcga tattctatca
    43321 ctcccgcctg attcctcaat gtagttcggg tttctacctt acgcattatt gttttcgttg
    43381 acaaattgcg caacacggtc tgttttgcga tcatcttgct taaatttacg acgaacgaac
    43441 aatttcttta gaaaatgcgt tgactcattt tgaactctcc ctataatgcg actccacaca
    43501 gcgggggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    43561 gatcccgtca tcacccacca actactttat gtagtctccg ccgtgtagca agaaattgag
    43621 aagtgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
    43681 gatcccgtca tcacccacca cttcgggtcg ttagctcagt tggtagagca gttgactttt
    43741 aatcaattgg tcgcaggttc gaatcctgca cgacccacca atgtaaaaaa gcgccctaaa
    43801 ggcgcttttt tactatctgc gatactcaaa gattcgaacc tgcagcaggt ttgagttgag
    43861 cgcagcgaaa caacggagcc gctcgcggcg acggcccgaa gggcgagcga agcgagtcat
    43921 cctgcacgac ccaccaatgc aaaaaagcgc cctaaaggcg cttttttgct atctgcgatt
    43981 tgtaagattg atttttcttc tcaagttcct tgttcttcta ccgatattac ctcgtacagt
    44041 gacgaggact gaacaatggc cactatctct tcaacttcaa ttccttccat acaaacacag
    44101 agtagtaaca gggcatcaca aggtagcgat gttgcttcac aaatcgcgcg gataagtcag
    44161 caaatcatca aactcactca acaaattaaa gagattgtag acactagcgg cagtgctgaa
    44221 gacaagcaaa aacaggccga attgattcaa cagcaaatca ccctactgga aacacaactg
    44281 gcgcagttgc agaaacagca ggcagaaaag gcgcaggaga aagagcagcg tttgtctctg
    44341 aatgtatccc ttctaaatcc tgttgaaaaa acgactcata tcgatatcta tatctgatac
    44401 aacggatccc cttcccgacc agccattagc gcattgccct ggtctcgtac cacgtcccat
    44461 aacgcttcag cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat caactggcgt
    44521 tcaaccaccg gcgtaatgcg cttaaccacc agtgggctac cttcaggcaa tggtagcgcc
    44581 agtgcaggaa gaatactgat gccgatgcct gcggctacca tcgggaacag cgtcgccgga
    44641 tgtccaatct cctgcacaat attcgcctga ataccattgc gcgccagcgc tgcgtcaatc
    44701 agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg taatgcctgc
    44761 cagggtacat aatcttccac ggccaaagcg ctatcgcgat ggcaaagcag aaagaaaggc
    44821 tcggaaagaa tcgcttcaca ttgcagatcg cccactgggc ctggatcgat gacgatgcca
    44881 aagtcgacat ctccctggcg aatactttcc atcacccact gctgcggacg atcatgcagg
    44941 acaaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc
    45001 gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg ccccatacgc
    45061 ccggtatcgc gcaacgtgct gttaagttca tccagcagtc gctcaagacg caaagccaac
    45121 tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag gcgaacacca
    45181 gtatgatttt ccagctcctt cacactgtga cttactgccg actggctcaa gccgatacgc
    45241 tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt taattgtttt
    45301 aaagaataat tcatctgttt tcttcatgaa tggatgcaat aaatcaattt tatttctcaa
    45361 actggaagaa gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat
    45421 cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg ctggcctctt tctttccggc
    45481 cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc gcagctattg ccctgctgtt
    45541 ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt gctggcggtg gtcactggcg
    45601 actgcatttg tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg gtgtactgtt
    45661 tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg
    45721 cattctcccg gctaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt
    45781 cgcggcggcg gtttgttctg cgtcagcatc cagcctgctg gggattttcc tttcaccatt
    45841 gctggttggt ctggtgatga acgttcacgg tgcaaggggc agccttgagc aggtcggtaa
    45901 aattatgctg caactgctgc tgccgtttgt gttggggcat ctttcccggc cgtggattgg
    45961 tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt
    46021 ggtggtttat acagcgttca gcgaagccgt cgttaatggt atctggcata aagttggctg
    46081 gggatcattg ctgtttatcg tggtggtcag ctgcgttctt ctggctatcg tgattgtagt
    46141 taacgtcttt atggcacgcc gactgggctt caataaggca gatgaaatta ctatcgtctt
    46201 ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac
    46261 atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt
    46321 ctgtgcggtg ctagcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga
    46381 aagcagcgcc gataaagctt aaagcggacg cttcaggggc tggaccagtt gcgtcagccc
    46441 ctcggtttta atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc
    46501 atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcaattaagc gacgcccttt
    46561 gtatttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc
    46621 agctacccag caaacgcagc atttccgctt cgtcgatgac ttcaatgccc agttcctgcg
    46681 ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca
    46741 cgctgcccgc gactttcgcg cccagttcga ccagtcgagc tttagcgtca tcacgcgaca
    46801 tctggcttaa gctgcccgta agcaccacgg ttttaccagc aaacgggctg tcaatctctt
    46861 ccgcgttgat aacgatcggc gcaggccagt gaacaccttc cgccaacagc tcgctgatga
    46921 cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg ggatgcaacg acaatgccaa
    46981 catcaggcac cttttgcagc tcttcaatcg aagcggcttc cagcgcttcc agcgtgccga
    47041 aatatgccgc cagacctgct gcggtggcct cgccgacttc acggatgcca agtgcataga
    47101 ggaagcgagc aaaggtggtt tctttcgctt tttccagcgc gttaaccacg ttttgtgcca
    47161 attttggccc catacgctcc agtccggtca gtttgcctgc ggtgagtttg aacagatctg
    47221 ccggagtatg gacatattct ttttcaacca gctgatcgat gattttgtcg cccattccgt
    47281 caacatccat cgcacggcgg gaaacaaagt gtttcagcga ctctttacgc tgcgcaccgc
    47341 aaatcaggcc accggtacag cgggcaaccg cttcaccttc cacacgctca acgtcagaac
    47401 cacataccgg acaatgcgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa
    47461 gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaatcacc actttatcgc
    47521 caatgcgtaa accaagacgt tcgatttcat ccgcattgtg taaggttgcg ttactcacca
    47581 gcacgccagc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc
    47641 caacctgaaa ctcgacgtca cgcacaaagg tcatctgctc ctgcgccggg aatttaaacg
    47701 ctaccgccca gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt tccagtgagt
    47761 tgaccttaat caccacgccg tcgatatcaa agcccagcgt cgggcggtct tcttccactt
    47821 tgtgatagaa cgccagcact tcttccgccg attcacaaag cgttacccga tcgctgaccg
    47881 gcaaccccca ctttttaaat tgcagtaaac ggccaagatg agtatccggc agctcgcccc
    47941 cttccagaac accaacgcca tagcagaaaa aagtgagcgg tcgcttcgct gtaatacgcg
    48001 gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaaa actttcccgc
    48061 ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac
    48121 cacgcacttc cagacgcgcc gggatattct ctccgtgcag cttcagcgga atggcgcgaa
    48181 tagtacgcac attagacgtg atatcttccc cggtggtgcc atcgccacgg gtcgcggcac
    48241 tgactaaaac gccattttca tacagaatac tgacggcaag gccatccagc ttcagctcac
    48301 agcaccaggt gactttctcg ttgtttttca gacggtcctg cacacgtttg ttgaaagcaa
    48361 gaaagctttc ttcatcaaaa acgttatcca gtgacagcat tggtacttca tggcgtatct
    48421 ggctgaaagc tgccagcggc gcagcaccta cacgttgggt aggcgaatca ggcgtaatca
    48481 gttctggatg tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg tattcagcgt
    48541 cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcaa agcgtcgttc
    48601 gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc
    48661 cgacaagcgg gggttcgaag aggagttaat ttgccttaag tgtatcaggc gttggcgtct
    48721 ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct gcggagtcat catactgcgc
    48781 tgatcgtcaa gcacgacacc gcccacttca tcggcaatat gctgcgcaga ttgcagcatc
    48841 agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc
    48901 ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg
    48961 ctgaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat attcatatcg
    49021 ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt tagctcgcta
    49081 ccgtgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata
    49141 actggagccg gttccgctac aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg
    49201 aaagcctgtt gtgccggttg cggtgctgaa tgcactggct gcggcgctgg cgcgacctgt
    49261 ggcgaaactg gctgctggac cggttgacgc ggctgcgcag acgcataagg cggttggtac
    49321 tggtgttgcg gcgacggacg agcagcctca tgctcctgag cgttagccgg ggcatgattc
    49381 acgcggtgaa cacgaacctc accaacgccc tcatcatctt cgacatcctc gtcataagaa
    49441 tcgtcgtcac gttttgactt cattcgtttt aatggccgat cgcggaacat agaagatcgt
    49501 tctttacggc tggtccagaa accatgtacc agtaaagcga ttatggcgat cgcgccaaca
    49561 atgattaata tcagacgcaa atcctgcatc attatattct ctgttgttct aacaccttgc
    49621 caccacggca aacatttact cactaagagt atttgccgat tacctcaagt gcaagtgcac
    49681 tattaacttt cacagcacaa agatagatga aatcgtgctt tttgctgttt tttcgaacat
    49741 atcctaactg tccattgcgc aattacccgg taaaatacgc agaattttcc tgggattggt
    49801 caaaaggagc tcatcctgat tatggtttca tcattcacat ctgccccacg cagcggtttt
    49861 tactattttg cgcaaggctg gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt
    49921 ttaccgctgc tggtcaatat tttgttgatg gggggcgcat tctggtggct ctttacacag
    49981 ctcgatgtct ggatcccgac tttcatgagt tacgttccgg actggctaca atggctgagt
    50041 tatctgttgt ggcctctggc tgtcatctct gtgctgttag tgtttggcta tttcttctcc
    50101 acgattgcta actggattgc cgctccgttt aacggtttat tggctgaaca actggaagca
    50161 cgattgactg gcgctacacc gccagatacc gggattttcg gtatcatgaa agatgtgccg
    50221 cgaatcatga aacgcgaatg gcaaaaattt gtctggtatc tgccgcgcgc aattgtatta
    50281 ctaattcttt acttcatccc tggtattggg caaaccgtcg cgccggtact gtggttcctg
    50341 tttagcgcct ggatgttagc catccagtat tgcgattacc ccttcgataa ccacaaagtg
    50401 ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt
    50461 gctttaacca gcctgtttac gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc
    50521 gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg
    50581 taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc
    50641 ccttattcca tcttgcatgt cattatttcc cttctgtata tagatatgct aaatccttac
    50701 ttccgcatat tctctgagcg ggtatgctac ctgttgtatc ccaatttcat acagttaagg
    50761 acaggccatg agtaagattt ttgaagataa ctcgctgact atcggtcaca cgccgctggt
    50821 tcgcctgaat cgcatcggta acggacgcat tctggcgaag gtggaatctc gtaaccccag
    50881 cttcagcgtt aagtgccgta tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt
    50941 gctgaaacca ggcgttgaac tggttgaacc gaccagcggt aataccggga ttgcactggc
    51001 ctatgtagct gccgctcgcg gttacaaact caccctgacc atgccagaaa ccatgagtat
    51061 tgaacgccgc aagctgctga aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa
    51121 aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc gccagcaatc cagagaaata
    51181 cctgctgctg caacaattca gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg
    51241 tccggagatc tgggaagata ccgacggtca ggttgatgta tttattgctg gcgttgggac
    51301 tggcggtacg ctgactggcg tcagccgcta cattaaaggc accaaaggca agaccgatct
    51361 tatctctgtc gccgttgagc caaccgattc tccagttatc gcccaggtgc tggcaggtga
    51421 agagattaaa cctggcccgc ataaaattca gggtattggc gctggtttta tcccggctaa
    51481 cctcgatctc aagctggtcg ataaagtcat tggcatcacc aatgaagaag ccatttctac
    51541 cgcccgtcgt ctaatggaag aagaaggtat tcttgcaggt atctcttctg gagcagctgt
    51601 tgccgcagcg ttgaaactac aagaagatga aagctttacc aacaagaata ttgtggttat
    51661 tctaccatca tcgggtgagc gttatttaag caccgcattg tttgccgatc tcttcactga
    51721 gaaagaattg caacagtaat gccagcttgt taaaaatgcg taaaaaagca cctttttagg
    51781 tgcttttttg tggcctgctt caaactttcg cccctcctgg cattgattca gcctgtcgga
    51841 actggtattt aaccagacta attattttga tgcgcgaaat taatcgttac aggaaaagcc
    51901 aaagctgaat cgattttatg atttggttca attcttcctt tagcggcata atgtttaatg
    51961 acgtacgaaa cgtcagcggt caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc
    52021 gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga
    52081 ctttagttcc acaacactaa acctataagt tggggaaata caatgttcca gcaagaagtt
    52141 accattaccg ctccgaacgg tctgcacacc cgccctgctg cccagtttgt aaaagaagct
    52201 aagggcttca cttctgaaat tactgtgact tccaacggca aaagcgccag cgcgaaaagc
    52261 ctgtttaaac tgcagactct gggcctgact caaggtaccg ttgtgactat ctccgcagaa
    52321 ggcgaagacg agcagaaagc ggttgaacat ctggttaaac tgatggcgga actcgagtaa
    52381 tttcccgggt tcttttaaaa atcagtcaca agtaaggtag ggttatgatt tcaggcattt
    52441 tagcatcccc gggtatcgct ttcggtaaag ctctgcttct gaaagaagac gaaattgtca
    52501 ttgaccggaa aaaaatttct gccgaccagg ttgctcagga agttgaacgt tttctgagcg
    52561 gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg
    52621 gtgaagaaaa agaagccatc tttgaagggc atattatgct gctcgaagat gaggagctgg
    52681 agcaggaaat catagccctg attaaagata aacacatgac agctgacgca gctgctcatg
    52741 aagttatcga aggtcaggct tctgccctgg aagagctgga tgatgaatac ctgaaagaac
    52801 gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg caacatcctg ggcctgaaga
    52861 ttatcgacct gagcgccatt caggatgaag tcattctggt tgccgctgac ctgacgccgt
    52921 ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt catcaccgac gcgggtggcc
    52981 gtacttccca cacctctatc atggcgcgtt ctctggaact gcctgctatc gtgggtaccg
    53041 gtagcgtcac ctctcaggtg aaaaatgacg actatctgat tctggatgcc gtaaataatc
    53101 aggtttacgt caatccaacc aacgaagtta ttgataaaat gcgcgctgtt caggagcaag
    53161 tggcttctga aaaagcagag cttgctaaac tgaaagatct gccagctatt acgctggacg
    53221 gtcaccaggt agaagtatgc gctaacattg gtacggttcg tgacgttgaa ggtgcagagc
    53281 gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg
    53341 cgctgcccac tgaagaagaa cagtttgctg cttacaaagc agtggctgaa gcgtgtggct
    53401 cgcaggcggt tatcgttcgt accatggaca tcggcggcga caaagagctg ccatacatga
    53461 acttcccgaa agaagagaac ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc
    53521 gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat
    53581 tgcgcattat gttcccgatg atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga
    53641 tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa
    53701 tcggcgtaat ggtggaaaca ccggctgccg caacaattgc acgtcattta gccaaagaag
    53761 ttgatttctt tagtatcggc accaatgatt taacgcagta cactctggca gttgaccgtg
    53821 gtaatgatat gatttcacac ctttaccagc caatgtcacc gtccgtgctg aacttgatca
    53881 agcaagttat tgatgcttct catgctgaag gcaaatggac tggcatgtgt ggtgagcttg
    53941 ctggcgatga acgtgctaca cttctgttgc tggggatggg tctggacgaa ttctctatga
    54001 gcgccatttc tatcccgcgc attaagaaga ttatccgtaa cacgaacttc gaagatgcga
    54061 aggtgttagc agagcaggct cttgctcaac cgacaacgga cgagttaatg acgctggtta
    54121 acaagttcat tgaagaaaaa acaatctgct aatccacgag atgcggccca atttactgct
    54181 taggagaaga tcatgggttt gttcgataaa ctgaaatctc tggtttccga cgacaaaaag
    54241 gataccggaa ctattgagat cattgctccg ctctctggcg agatcgtcaa tatcgaagac
    54301 gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg gtattgctat caaaccaacg
    54361 ggtaacaaaa tggtcgcgcc tgtagacggt accattggta aaatctttga aaccaatcac
    54421 gcgttctcta tcgaatctga tagcggcgtt gaactgttcg tccacttcgg tatcgacacc
    54481 gttgaactga aaggcgaagg cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt
    54541 ggcgatactg tcattgaatt tgatctgccg ctgctggaag agaaagccaa gtctaccctg
    54601 actccggttg ttatctccaa catggacgaa atcaaagaac tgatcaaact gtccggtagc
    54661 gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa
    54721 atggcgccca tcggcgccat tttttatgct tccgccagcg gcggcaaaat caattcatcg
    54781 ctctcatgct gctgggtgta gcgcatcact tccagtacgc gcaaccccgc acggtgcacc
    54841 gcatcgttta acgccttccc tttcagcaaa ccactgataa gctgagcaca aaacaggtcg
    54901 ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa tgacattcac gctgtcggca
    54961 ctgaccacca ccacctgcat ctcctgattt tcttcattac cggaggcgct ggtaatcacc
    55021 acccatttta atgtgtctga aagcagactt tttgcggcag caatggcact gtcgagatcg
    55081 cggcaatttt taccggtcag gatttccagc tcaaagatat tgggggtaat tccctgcgcc
    55141 agcggcagta aatattgtcg atacgcctcg ggaaggtcag gtttgacata aattccgcta
    55201 tcaatatcgc caatcaccgg atcgaccatg atcaataggt caggatggtc tttgcgtagc
    55261 gcagtcagcc actcggcaag gattttgatt tgcgatgccg ttcccatata gcccgtggtt
    55321 acagcacgaa gttggcgcag cgcatcacgc tcctgaagcg cacgcaaata gccgctaaac
    55381 cattcgtccg gaatcgcacc accgtagaaa gtgtcataat gcggcgtatt gctcagcaat
    55441 accgtcggca cggcaaagac attcaagccg ttctgtttta tagcaggcac cgcaatgctg
    55501 ttgcctacgc ttccgtacac tacctgcgac tgcacggcga cgatatccgc ctgcagcgcc
    55561 ctactcttat cgttaaacaa caacaaacta ctcatttaat tttttctcct tgccgatgat
    55621 cctcatcgta ttccaaccga aactttacct gattctggca gtcaaatcgg ctattacaaa
    55681 acaaagataa ggtaattcag tgaagaaaat catttgtctg gccattacgc tgctaatgac
    55741 actccccgct tacgcgaagt taactgttca tgaagaagcc cgcatcaacg ccatgctgga
    55801 gggattagca cagaaaaagg atttgatatt tgtgcgcaac ggtgatgaac atacctgcga
    55861 tgaagcggtt tcccacctgc gtctgaagct cggcaatacc cgtaaccgca ttgacactgc
    55921 cgagcagttt attgataagg tagcttcgtc gtcatcgatt accgggaagc cgtatatcgt
    55981 gaagatcccc ggtaagagcg atgagaacgc acagcctttt ttacatgcgt taattgcgca
    56041 gacggataaa acggtgcctg cggaaggaaa ttaatccgct ttggtaaggc atttacagga
    56101 ggtaacatga aaaaatgctt tatttatcac gatgaaaaat cgaataaatt ttggtggata
    56161 gattacgaag gggatagttt agctgtcaac tatggcaagg taggtagtat tggtaaattc
    56221 catacaaaag aattcgataa tgaagaacag tgtctgaaag aagccagtaa attgattgcc
    56281 gcaaaaatga aaaaaggata tcaagaaact ccagacttta acttcatgga tctctactat
    56341 tttgatgatg aagagattgg gttacatctt aaaacgtcac acccaaactt ccagtgccat
    56401 tttactgatc cactttatat gtgttgctgg gatgaagaat ctccttttgg tagcgatgaa
    56461 ggtgctgatg ctctaaacga acttgaaaat agcctccgtt ctggactgtg ctgatttccc
    56521 tcaaatgtta attgaaacta tgtggggtat gaaatacatc gctatggaca gtattcttga
    56581 agaggatgtg cgtgcgcaat tactagccga tgaaatgagc tctatccaga gcaatatgat
    56641 tacctacgcg actgcattcg gtcagatcaa agtcatgggt aaaatctccc ataaacttaa
    56701 aaagatggga ctcaatgcac tagcgcgtca tcagcttacc gcaaaaattc ttcaatgggg
    56761 tgacggtcag gactcaccaa tacttcaaaa aatgattgat gaccttactt cttttccaca
    56821 cgaaaattaa aaactacatt tgtcggcagc aacaactgcc gacaaatcag attattgaca
    56881 aagtgcactt tgtttatgcc ggatgcggcg taaacgcctg atccggcata aaaatattgc
    56941 taattcaata tattgcagaa acattgtagg cctgataagc ggagcgcatc aggcagtttt
    57001 gcgtttgtca tcagtctcca atgctattaa tccttaaatc cctgccccct gactaaaatg
    57061 ctcttcccca aacactccgg tggagaggta gcgatcgcca cgatcgcaga tgatcgctac
    57121 caccaccgca ccagggttag cttttgccac ccgcagtgct ccagcaaccg cgccgcctga
    57181 gctgacgcca cagaatattc cttcccgcac cgccagttcg cgcatggtgt tttccgcatc
    57241 gcgctgatga atatccagca cctcatccac cagcgcagcg ttgaaaatcc ccggcagata
    57301 ttccgcaggc cagcggcgaa tgcccggaat actgctgcct tcttccggtt gcaggccaac
    57361 aatggtcacc ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt
    57421 cgtccccatg ctggagacaa aatgagtgat gcgcccgccg gtttgctgcc agatttccgg
    57481 cccggtggtg gtgtaatgcg cataagggtt atcgggatta ttgaactgat cgagcagctt
    57541 tccttcgcca cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc
    57601 tttggtgaca agaatcagtt ccgcaccata agcatgcatc gccgcacggc gttcctggct
    57661 catgttgtcg ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag
    57721 cgcaatgccg gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc
    57781 gcgcttttcc gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg
    57841 gttattgcct tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa
    57901 cttcaccaga ggcgtattgc ctattgtttg ttctaatgta ctcacgatct ctatccatac
    57961 gtggtgttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacaggtta caaaccttgc
    58021 cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc
    58081 aggcgctttg tgcgagagca agttcctcat cgcgggtttc aatacgctcg tcgccgttat
    58141 acagccgcgc atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc
    58201 catgcatcac gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg
    58261 tgtagtgacc tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg
    58321 tacggcggct gatatccact tcccacgggc gcaggaagag atccaccgga ccctgatacg
    58381 caggtgtgta gcccagcggc cagcgatgcg cgccaacatg gaactgcccg ccgcgaatgg
    58441 ttccctgcag gcggttcact tcgcccataa attcgagtac aaaacgggtc gctggttcgc
    58501 gccatacctg attcggcgca tcagcctgtt caatattccc ctgactcatc accactacac
    58561 gatcggctac ttcggtcgcc tcttcctggt cgtgggtcac aaacacgctg gtgaatttca
    58621 gctcttcatg cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg
    58681 cgccaaacgg ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg
    58741 ccacacgctg tttctggccg ccggaaagct gcgccggata acgatccgcc agatgcgcaa
    58801 gctggaccat ttccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgct
    58861 cgcgacgcgg cagcaccgtc aggccaaaag tgatattgtc gaataccgtc atatggcgga
    58921 acagcgcgta atgctggaac acgaaaccga ctttacgatc gcgcgcgtgc aggcgactca
    58981 cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatcc
    59041 gcagcagcgt ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgaccagaag
    59101 gaatatccag tgagatatcg ttcagcacct gggtgcgacc aaaagacttc ttgatattgg
    59161 caatctcaat gctcatgatg ctcctcctgc tgcgcgcgtt tttcctgatt ctccaggcgc
    59221 cactgcaaca tactctttaa aaacagggtg ataatcgcca tcagcgtcaa cagcgcggcg
    59281 gcggtaaagg agccgacggt gttgtagtcc tgctccaaca attcaatctg taacggcagc
    59341 gaaagtgttt cgccgcgaat cgagccggaa accaccgaca cagcaccaaa ctcgccaatc
    59401 gcgcgggcgt tggtcagcac cacgccgtaa agcagcgccc agcgaatatt cggcaatgtg
    59461 acgcgacgga acatctgcca gccggacgcg ccaagcaaaa tcgccgcttc gtcttcctgg
    59521 ctgccctggc ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag
    59581 atggtgacca gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc
    59641 cagccgccga gcgggccgtt ggagccgtag aacagcagat acaccagacc ggcaaccacc
    59701 ggcgatacgg caaacggaat gtccagtagc gtcagcagta actggcgtcc cgggaagtta
    59761 aagcgcgtca ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc
    59821 agcgcgatca tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt
    59881 aaaaccggca tcagcccctt gctgaatgcc tgcacgaaga tgtaaatcat cggcaccagc
    59941 aggatgaacg ccgaaaccag catcccgatg ccaatcagaa accatttgcc ccagttaatc
    60001 gggcgcgcgt cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc
    60061 gaccaaagcg actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga
    60121 tcaccgaagc aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca
    60181 tcagcgacgt cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa
    60241 attcaccaag actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagct
    60301 ccggcagcac cactttgcag aaactctgcc agcgcgttgc accaagcgtt tccgccgctt
    60361 cttcatattc cgggcctaac tcttccagca ccggctgcac ggtacgcacc acaaacggaa
    60421 tgctggtaaa ggccatcgcc accgcaatac caagccaggt ataggtgact ttgatatcaa
    60481 acttcgccag ccattcaccg taaaaaccgt ttacggaaaa gagcgaggcc agcgttaaac
    60541 cggcgactgc cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc
    60601 ccggaaagcg ataacgtgtt aaaatccacg ccatcagcag accgaaaacg ccgttaaaaa
    60661 tcgatgccac aaacgccgac agcagcgtca ctttgtaggc cgcgaccacc tgcgggttgg
    60721 tgatcacctc ccagtactgc gcccagctca tctcagaaag ctgcatcacc agcgcagaga
    60781 gcggcagcag caatatcagg cacacaaaca gcagactggt gccaaggctt aaagtaaagc
    60841 ccggcagcac gcgtcttgaa gaaactgcaa acattactta cgccccgccg ctaacagctt
    60901 gtctaactcg ccgccgctgg tgaagtgggt tttcatcact tccggccatg agccaaattt
    60961 gtcttccacg cggaacagtt cggtctgcgg gaatttatct ttcagtttgt ccatgacttc
    61021 cgggttattc acacggtagt aatagtcggt gatgatggtc tgcgcctgtg ggctgtagag
    61081 ccagttcagg taggcttttg cggctttttc cgtaccgttg gcctgcacat ttttatcgac
    61141 ccacgccacc gggaattccg ccagaatatt ggttttcggg atcaccactt caaagccctg
    61201 tgcttcatac tgtttacgga tgttgttcac ttccgattca aagctaatca gcacatcgcc
    61261 caagccacgc tcggcaaaag tggtggttgc gccacgaccg ccggtatcga acacttcaac
    61321 gtttttcagg aactgggtca taaactgttc ggttttggct tcatcgccac cgtcagcttt
    61381 atccgctgcg ccccatgccg ccagataggt ataacgcgcg ttacccgacg ttttcgggtt
    61441 cgggaaaatc agcttcacgt cggagcgcac caggtcgttc cagtcgtgga tattcttcgg
    61501 gttgccctta cgcaccagga agcccatggt ggagtagaac ggcgagctat tattcggcag
    61561 gcgcgactgc cagtcagccg ggatcagctt gcctttgtcg tgcaggattt gtacgtcggt
    61621 cacctggtta taagtgacaa cgtctgcttt taagccctgc aaaatcgcca gcgcctgttt
    61681 tgatgacccg gcatgagatt gttttatcgt cagtttgtcg ccgccgttat cttttgccca
    61741 ttgctgttca aacggcggat tcagggcggc aaacagctcg cgggagacgt cataagaact
    61801 gttcagcagt tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag
    61861 tgagttcttt ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct
    61921 aatagccatc atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg
    61981 atttggaagt taaaaaggga ataagaactt ggttcaacag gcgtaattgt tgcaggcagt
    62041 ttggacacgg acagcgcgga gaaaccggag cgtacacata gtacgtgagg atttcgagca
    62101 ctgcccgggg ccaaaatgac aaatgaaata gcctggtgaa cttagttcaa gacgaaatcc
    62161 tccccacaca tgcagggagg aaacagaggt gaatcagata ccgacgctaa ccgtctccgg
    62221 cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt aaatagctgg attcatccga
    62281 tgcgaggaag gccgccagtt cgccgacttc cagcggatcg gcgaggcgac acagcgggat
    62341 tgcttttgcc atttcagtca gcaccgattc tggatcttcc gggttcgact ggcgggcaat
    62401 gctttccgcc attggcgtgc ggacgtatcc cgggcagatg gcgttaacgc gaataccgga
    62461 ttgcgcgtac tccaccgcca gcgatttagt caggccaaca atcgccgctt tcgttaaggc
    62521 atacgccgtt tcgccaggat cggccaccat atcaccagtg actgaagaca tcatcacaat
    62581 gcgaccatct ttgcgcgcaa tcatctccgg cagcaccgcc ttcgtgacgt tccatacgcc
    62641 tttaatattg atatcaatat ggaaatcgcg atcttcatcg ctcatatcga ggaaactgcc
    62701 cagacgacaa acgcctgcgt tattcactag gatatcaatg cgcccttctt tttccttcgc
    62761 gcgtttgata gctgcggcta ccgacgccgg gtcacgcaca tcggcgacaa ccgccgtaca
    62821 gcgatgacca cgaccacaca gttcgtccgc tagcttttcg atctcagggg agatatccag
    62881 caagattagg ttcgcgccat gacgtgcaaa agttctggca attccttcgc caattccctg
    62941 caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta cccattttaa tgctctcctt
    63001 gttaatacag agcgttacac tccattccct ctgaaaatac aggataaaca aaatattgct
    63061 gcggatcagc tcactaattc aggaaaatct caatccagtc gctgctttat gcatcatttc
    63121 aggcgttgtg ttagctcact gctgcgctga atcattttca atgacgccac atcattgatg
    63181 ttgcacaaga ccaacaaaca gtaaatcggt aacagaattt tgcgccgtgc gggtagacat
    63241 cgacgagcta cgccattcgg tttcaccgga aacggtatcg agcgtgaaat gcgccagccg
    63301 ccgcagcgga gagtcagcca gagaagtaat ggcaatcacc gttgccccct gctttcgtgc
    63361 cgcttctgcg cacaagacaa tctctttttt actgccgctg taagagatag cgatttgtac
    63421 atcgcctttt ttcaacgcct gggaaacggt ggcttgtacg tgcgtgtcgg cttcgcaggc
    63481 cacgcgatag ccaattttca tcagtttgaa cgacagatcg cgccccacca gcgccgatcc
    63541 gcccaggccc gttatctgga taaacggtgc tttactgatg acctcaatga ttttttgcag
    63601 ccgcgcgtag tcgaacaacg cacaggtttg ctccagcgcc agctcttttt cgcgattcag
    63661 tttgcgggca atcacttcca gcgaatcatc actggtaatc gaactgtgca ggtgcagcgc
    63721 cgtggcattc gttttctccc ggctggcact gtattcgcca attaacgcca tacgcaattc
    63781 agtaaatcct tgcgcgccga gcttctgggc aaatttcaca atgctcgact ggctgatacc
    63841 gagctgtttc gccatctgcc gtgaagagac ggatttcagc tcgctcactc tggcccgcag
    63901 aaagtcagca attttttgct cgttcccggt aaattcactt tccgcattgc gtattttcgt
    63961 caggtacaac atcttccact ctcctgggct ggcagcaaaa attccgctta agttcttatg
    64021 acgctcttca cactctgcga gtgtagactc aatgattcct ttaagtctgt caattcggga
    64081 tatatgattc catacatctg caattatttg atcgtaaata gtaaggtcac caccgatgca
    64141 actagaaaag atgattactg aaggctcgaa caccgcctcg gctgaaattg accgcgtatc
    64201 gacgctggaa atgtgccgga ttatcaacga tgaagataaa accgtaccgc ttgccgttga
    64261 tcgcgtcctg ccggatatcg ccgcggcgat cgatgttatc cacgcccagg tcagcggcgg
    64321 cggtcgtctg atttatctcg gtgcgggaac atccggtcgt ctggggattc tggatgccag
    64381 cgaatgtccg cccacctacg gcgtgaaacc gggtctggtg gttggtttga ttgctggagg
    64441 cgaatatgcc attcagcacg cggtggaagg cgctgaagat agccgggaag gtggtgttaa
    64501 cgatctgaaa aatattaatt taacggcaca ggatgtggtg gttggcattg ctgccagcgg
    64561 tcgcacgccg tatgtgattg ccggactgga atacgcacgc cagctcggct gccgcacagt
    64621 gggaatttcc tgtaatccgg ggagcgccgt ttcaaccacc gctgagtttg ccattacacc
    64681 gattgtaggt gccgaagttg ttaccggttc ttcgcggatg aaagcaggca cagcgcagaa
    64741 actggtgctc aatatgcttt ccaccgggct gatgattaaa tccggcaaag tgttcggcaa
    64801 cctgatggtc gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg tcaatatcgt
    64861 taaaaacgcc accggatgta atgccgaaca agcggaagcg gcgttaattg cctgcgagcg
    64921 caactgtaaa acggccattg tgatggtgct gaaaaatctc gatgccgcag aagctaaaaa
    64981 acgcctggat caacacggcg gctttattcg tcaggtttta gacaaggaat aacccatggc
    65041 caaagagatc agcagtgaac ttctgaacac cattcttacc cgtgtcggcg gaccgggaaa
    65101 tatcgccagt tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg acagttcact
    65161 ggttgatccc aatatcaaaa cgctggaagg cgtgaagggc gtcattttga ccagcgacca
    65221 ggtgcaggtc gtttttgggc ctggtaaagc gcatcgtgcc gcaaaagcga tgagcgagct
    65281 gctgggcgag gcaccggtac aggatgccgc agaaatcgcc gcccagaaca aacgtcagtt
    65341 aaaagccaaa caaacctccg gcgtgcaaca atttctcgcc aaattcgcca ccatcttcac
    65401 gccgctgatc cccggtttta ttgccgccgg tctgctgctg gggatagcga cgttaattgc
    65461 cacggtgatg cacgttccgg cagatgctca gggcacgcta cccgacgcgc tgaattttat
    65521 gaaggtgttc agcaaaggtt tgttcacctt cctagtgatt ctggtgggct ataacgccgc
    65581 ccaggcattt ggcggcacgg gcgtcaatgg cgcaattatt gccgcactct ttttgctcgg
    65641 ttataacccc gctgcaacca ccggttacta cgccggtttt cacgatttct ttggtctgcc
    65701 catcgatccg cgcggcaata ttatcggcgt gctgattgcc gcgagggcct gcgcccgcat
    65761 tgaaggcatg gtgcgccgct ttatgccgga cgatctcgac atgctgctga cctcgttaat
    65821 caccctgctg atcaccgcta cgcttgcgta cctgatcatt atgccgctgg gtggctggtt
    65881 attcgaaggt atgtcatggc tgtttatgca cctgaacagt aatccgttcg gttgtgcggt
    65941 tttagccggg ctgttcctga tcgccgtggt atttggcgtg catcagggct ttattcctgt
    66001 ttacctcgcg ttaatggaca gccagggatt caacagctta tttcccatcc tttcaatggc
    66061 aggcgcgggc caggtgggcg cggcgctggc actctactgg cgggcgcaac cgcacagtgc
    66121 gctacgcagt caggtacgcg gggcgattat tcccggcctg ctgggcgttg gtgaaccgct
    66181 gatttacggt gtcaccctgc cccgcatgaa gccgtttgtt accgcctgtt taggcggcgc
    66241 ggcgggcggt ttgtttatcg gattgatagc ctggtggggt ctgccgatgg gcttaaacag
    66301 cgcctttggg ccgtctggtc tggtggcgct gccgctgatg acttccgcac aaggcatcct
    66361 tccggcaatg gcggtttatg ctggcgggat tctggtggca tgggtttgcg ggtttatttt
    66421 caccacgctc tttggctgcc gtaacgtcaa tctggactga tattatgaaa cggacaatgc
    66481 tctatctttc tctgctggct gttagttgta gcgtcagcgc cgcaaaatac cctgttctga
    66541 cagaaggtag ttgatcaagt atatgcggcg ttaaagcagt aataaaaagg ccagtcagta
    66601 atgttactgc ctggccttta atcaatgaat cagaaacgct tacagcacca tcaacttgtc
    66661 cagcgacggt gcgaaataat agccgccggt taccggtttg gtgaaacgca acatcgcatc
    66721 acgtttgcca tcggtatcgc caaacatgct cagcagttgc tgctcaatgt tatgcagacg
    66781 cgcgcagtag gcgcagaagt acagaccgtg agtgccactg gcggtgccgt acggcaggct
    66841 ctggcgaaca atcttcagcc ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga
    66901 ggtttccgga cgttcgtcgc cgtcaatctc ttcgttggcc tctttggtgc gcccgatcat
    66961 catctcctga tcgtgaacgc tcatccggtt gagctgcttc aggttgtgct cccaacgctg
    67021 cacaaacaca tagctgccgc cggcatccac gccgtctttg ataaccgcca cttcgcggcg
    67081 cgtctcttca cccgccgggt tttctgtacc gtcaacaaag ccgctcaggt cacgctcttc
    67141 cacccaacgg aagccgtgga tctcttcttt cacttcaatg cagtcaccaa aggcttccat
    67201 cgccgcctga gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac
    67261 atcgaactgg gtcgtcggcg caaggccttt accgtagccc ggaaaatctt tcagctcttc
    67321 tgccccaacg ccgccgctca gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc
    67381 aagatgcgcg tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa atgttttact
    67441 ggccgcacgc agggcgtcaa cttccccttt cacgttggct tcgatccaaa tcgccgcgcg
    67501 gcaatgttct ggcaaaatac cactctgaac ctgagacatt gttcctcctg aaaataagaa
    67561 tgccacgcaa tccgtggcat tgttgagcgc tattttacgt gttttttcgc gacggcggtt
    67621 tgttcagacg caaattaacg ccgccagata attttactga ctttccagtt tttcagggta
    67681 tcgtccgaag gcattaaccc ttcaggacca cgccattccc cgctgaactg gtagctaata
    67741 tgttgactgc cttcggcttt acattcgacg gcacgattat catcaccatc agctttttgg
    67801 caattgccaa atgctttgct gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg
    67861 tcagcaggaa tatcgctatc cagcacgtca atgcgactga tcgtgccttg atcgccgtta
    67921 atcaccatcg ccacgttgtc gcctttcatc acttcaaaaa agcgcaccac gttgccgttc
    67981 gcggttttca ttccgctgcg caggcgataa tcgccatcaa gcgcatcagc aatggcttgt
    68041 tcttgcagtg gtgtggacgc cgttaattca cccacgccct gctcgctcac tttggtggac
    68101 gatccaaacc agttccacgg gttagcggca gaccagttaa ctgacgacat cgtggaacag
    68161 ccggtcagca tcaacggcat agcgcataac attaaacgca gcgatttcat gtcacttcct
    68221 ttggttattc aataacgttg cttggagtgc aaattcacca aaaagtgccg ttattctttt
    68281 tcttcattaa aacaagcgcg taaacgtcga ttggtcagca gccagattaa cgccacaata
    68341 tccgccacta ccagcgccag accgataccg ctaacggatt caccgttcag ccacagccac
    68401 ggttgccagc aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag cacatgccag
    68461 gtgcgaggaa acgaagcccg ccgaccgctc aacaaaaacg ccagcaccgc cggaataccg
    68521 ggaatcagcc ccagccagaa attatcgtga tcgggataaa acagatttag cagcgcagta
    68581 ccctgctcgc gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat
    68641 agcacaagcc agaagagcaa gggtaaacgc aggcgtccgt gcgcatcata atggacagga
    68701 tggaactcag tactcttcat cttcaatcaa acgcttaccc agactcagca cgtcggcgtg
    68761 ttcatatccc aggcgttcat acattccgag caccatgtcg ttatcttccg gcacgttgat
    68821 ctgaattttc gggcagccgc gagcaatcag ctttttctcc agccggttaa gcaacgcatt
    68881 ggcaattcca cgcccacgaa actctggatg cacgccaaga taataagcag acccgcgatg
    68941 cccgtcataa ccgcccatca ccgttccgac cacttcaccg tttacctcag cgaccaaaaa
    69001 caaactgacg tcatggttca tcttacgctc gatgtccatt tccggatcgt tccacggacg
    69061 cagcaagtcg caacgctccc aaagggtgat gacctcttcg aaatcttcct ggcgaaatac
    69121 gcgtatctcc atggtattcg ttaccttttt gcgggttaaa aggctgatta tggcgtgaac
    69181 ggtcgaatta gccaatatct gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt
    69241 caactattga aatgaaaagt aaaacaattc tcaacagcaa accgtcgtaa cggattacgc
    69301 gatacgatat aacatctgga actttattat tacaactcag gccgtatgag cacttttaaa
    69361 ccactaaaaa cactcacttc gcgccgccag gtgctgaaag ccggtttagc tgccctgacg
    69421 ttgtcaggaa tgtcgcaagc cattgccaaa gacgaacctt taaaaaccag caacggacac
    69481 agcaagccga aagcaaaaaa atctggcggc aaacgtgtcg ttgttctcga tccaggccac
    69541 ggcggaattg ataccggagc gatcggacgc aacggttcga aagaaaaaca tgtggtgctg
    69601 gcgattgcta aaaacgtccg ttccattttg cgtaatcatg ggattgatgc gcgtttaacg
    69661 cgttctggcg atacgtttat cccactttac gatcgcgttg aaatcgccca taaacatggc
    69721 gcagatctgt ttatgtcaat tcatgccgat ggctttacca acccgaaagc tgccggtgct
    69781 tcggtatttg ccctctctaa ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa
    69841 cgcgaaaacc gcgccgatga agttgccggt aaaaaggcga ctgacaagga tcacctattg
    69901 cagcaagtac tgtttgatct ggtgcaaacc gatactatta aaaacagcct gacgcttggc
    69961 tcgcatattc tgaaaaagat taagccggtg cataaactgc acagccgcaa caccgaacaa
    70021 gccgcgtttg tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga aacctcgttt
    70081 atcaccaacc cggaagaaga gcggctgtta ggcacggcgg cgtttcgtca gaaaatcgcc
    70141 acagcgattg ctgaaggcgt gatcagttat ttccactggt tcgacaacca gaaagcacat
    70201 tcgaaaaagc gataagttat gaaacccgac gcacaccagg ttaaacagtt tctgctcaac
    70261 cttcaggata cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt tgtcgaagat
    70321 agttggcagc gcgaagctgg cggcggcggg cgcagtcggg tgttgcgtaa tggtggtgtt
    70381 ttcgaacagg cgggcgtcaa cttttctcac gtccacggtg aggcgatgcc tgcttccgcc
    70441 accgctcatc gcccggaact tgccgggcgc agtttcgagg cgatgggcgt ttcactggta
    70501 gtgcatccgc ataacccgta tgttcccacc agccacgcga atgtgcgttt ttttattgcc
    70561 gaaaaaccgg gtgcagagcc cgtttggtgg tttggcggcg gcttcgattt aacccctttc
    70621 tatggttttg aagaagacgc cattcactgg caccgcaccg cccgtgacct gtgccagcca
    70681 tttggtgaag acgtttatcc ccgttacaaa aagtggtgcg acgattactt ctacctcaaa
    70741 catcgcaacg aacagcgcgg cattggcggg ctgttctttg atgatctgaa cacgccagat
    70801 ttcgaccact gttttgcctt tatgcaggcg gtaggcaaag gctacaccga cgcttattta
    70861 ccaattgtag agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa ttttcagctc
    70921 taccgtcgcg gtcgttatgt cgagttcaat ctggtctggg atcgcggcac gctgtttggc
    70981 ctgcaaactg gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact ggtacgctgg
    71041 gagtatgatt atcagccaaa agatggcagc ccagaagcgg cgttaagtga gtttattaag
    71101 gtcagggatt gggtgtaact ccctcccccc cattcccgca tccgctgatg caacgtcaac
    71161 gacggcttct cggcaaacag ctgctggtaa tccgtggcaa attgccccag atgccagaat
    71221 ccccactgca tggcggcgtc ttttaccgtt gtgctttgcg accacgggct tatcagttcg
    71281 cggcgtacgg cgttcaggcg aatgcgtttc agccacgcgt ttgggccaat gcctaaaata
    71341 gcgtgaaacg cgttttgtag cgtgcggcga ctgacatgca gttgattaca caaatccagc
    71401 accgtcaccg gttcggacat gttttccagc acatattcac gggcgcggga aagcaaccga
    71461 cggtaactct gatgactgat gctttccgcc gtcaccattg gttgcgcttc ttccagcatc
    71521 gcccccatcg ccattagcaa attatccccc agcacttttc gcactgcagg ctgatggaga
    71581 ttttccgggt tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca cagcgcggct
    71641 ttatgctgct ctttcacttc cagcgccgac tggctacgca gcatatgtaa tacccgatcc
    71701 ggattgtgca aaaagttagc ctgacaggtg atgacatctt cagacagcac tacgcccagg
    71761 atcgtataat cgtccggcgt acttaattca aactcggttc caccggggcg cgttgctatt
    71821 tctgcgcttc ccagacattg cgaaccgata aatccctgct caccgcgcgt cgccggaatg
    71881 ccaaaccaga acgagttcgg ccagaccagg cacgactgac gcagcgccag accggtgtat
    71941 tcacgaaaaa cctgaatatc atcgagtaga atttccgtaa attcaccatg aaacttgccc
    72001 ggatgcagct gatcgtaaat ctgctgccag gcggtaatcg tcaaagcatg ttcatagaca
    72061 tccgttgtct gtcgttgatg aacattatcc acttcgacct tcggcgtgag cttcaggttt
    72121 tcgggtaagg gttcatgata aagatggtgc aaattggctg tacgggtctt tttcatgatg
    72181 ttaatgccgg gtgttgtagg acacccggca cctccgacag gttaatgggg cttgagacga
    72241 taacgactac tgcgtttacg ttacgtcccg gcagaaaaga gctgttcgag cgcatttctg
    72301 gctttttcca gcggccagcc aaagtgggcg gccacttctc ctgccgtcat tccctgacat
    72361 actgatgtta acagcgccaa caactcgtca gcagattccg cgacgataac tggcgcgtcg
    72421 ggttccttta cgggttgctt cggctggcgg ttaaagccag taaccagcca ctgggtgtca
    72481 tcgtctggcc gaccgatctc cttacggctg atgacacgcc cggtacgcat cgcggcagca
    72541 caacctgcgt ccagcgccgc acgacacgcc gccagatcgc cttccaccac cagcgttaag
    72601 cgaccagggt caagcacttc gtgactgagc agacgaacgt tagctgcttt gagcatggca
    72661 tccgctgcat ctatcgcggc gaccatgccg tccacttcca gcaatcccag tgcattgatc
    72721 atcggcaacc tccgttacgc acgctggatt ggattacgcg cgatttccag cactgcatcg
    72781 gtaaaggcgt tacaggctgc tttacacgcg gcctggctac cggttaaaaa tgctgccgag
    72841 tagttggttt cagacggtgg cgggacgtag gtcaccagct gtacgtcggc agatttcaac
    72901 gcggcatcaa tgccgtaggt cgcttccagc ggcggtgcca ccagatacgc catcggatcg
    72961 ccaagcgtga tcccggcggt tgatgaaaga taagaacctg tacgcgaaac tacatgcgcc
    73021 aggaaggcgg tgttttctgc gtcattggcc cactggaaag ctgcgccatt ttcaatatgt
    73081 gcaaccatcg catccagacc ggcacgcact tccgccgggt ttggcccacc aagcataatc
    73141 agcacttcac cggcagtcgg tgacgggccg tgtgccgctc ccgcatacag cgagcgacca
    73201 tataccactt cgaccatcgc ttgtttggtc gcttcgtcag ccgcaatgta ggttacgtcg
    73261 tcagaatcgg cagaaatcag cccgaggcta cgaatatgcg gcggcaattt tagttcacgc
    73321 gcaaattcag cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa
    73381 tctaaagctg gcatgatgcc tccttatcgg gtcatgttga tgccggatgc tttctgctcc
    73441 agcatacgtt tcgccaaatc cacaatgacg gctgcggctt caaccggcgg cgtgccgccc
    73501 tggtgaatgt tagagataca ggtgcgatcg gcctcaacgg tggtcgccat acgcggcgag
    73561 taaacggcgt agcaggagag gctttctgac tgccctaacc ccggacgttc ccccaccagc
    73621 aggatcacca ctttcgcgcc gaggatctca ccgatctgat cttcaatctt cacgcgacca
    73681 taacgaacaa agaatggcgt gccgactttc agcccggcct gtttcaggcc cgccatcagc
    73741 ggcggcagga tctcttcgta gttgacggtg atggcatcag ttgacaggcc atcagaaata
    73801 acgacctgta cgtccggatt agcaacacac tgtgctttca gcgcttcaac agcttctgca
    73861 cacaggcggc ggcccatatc cgggcgagtc aggtagagat ttttgtcgct aatttcagag
    73921 cgtacttcca gcaagccctg cgctttcacc cactcttccg gcacttcttt cagtacggta
    73981 tctttcgaac gagagtgatc ggccaagaaa cgcagcagcg cctgagtacg tggacgcgga
    74041 ccggcacgac cggtacaaac gcgggccacg gtgctgcggc gcagttctgt taatacgtct
    74101 gcgcgatgcg ggttttcaac accaatccac gcttttgctt cagcggaacc taaatccagc
    74161 gcacagcttt ccgaggtcac cggtgccgca cagttggtgg tggcgcactt tgcttctgac
    74221 ggcgcggggg ccgcttgtcc cattgacgcc atcacgctgc gtacaatttc ttcaatctgt
    74281 ttttgatcca tgatgtgtta tctccgcgtc atcagaagaa cagtgacgga tcgcccgccc
    74341 gtttggtcag gcgaccgttt gccataatgc ccatgctttc cagccagcgt tcaaactctg
    74401 gtgacgggcg caggttgagt aactgacgca cagtggcggt atcgtggaat gcggtggtct
    74461 ggtagttgag catgatgtca tcacccagcg gcatccccat gatgtagttg cagcctgcgg
    74521 tggcgagcag gatcatcagg ttttcgttga ggttctggtc agagtcagcg tggttggtgt
    74581 agcagccgtc acagcccata gagatgccgc tcagcttgcc cataaagtga tcttctaagc
    74641 cagcacggat aatctggcgg tcgttgtaga gatactccgg cccaataaag ccaaccacgg
    74701 tgttgacgat aaacggatcg taatgacgcg ccagcccata gttacgtgct tccatcgtca
    74761 cctggtcagc gccgaagtta gcgccagcgg atagcgcaga gccttgtccg gtttcgaagt
    74821 agaggcagtt ttccccggcg atacgattga actctgcgcc cactgcgcgc gcttcgtcga
    74881 gcatcgccag ttccacgcca aactctttca gccctttttc gctgccacag atactctgga
    74941 aaatcagtcc gccaggagcg ccgcgacgaa tcgcttcgat ctgggtggtg acgtgcgcca
    75001 gtacgcagcc ctgagttggg atgttgaatt tgtcgatcac gccataaatg gtatccagca
    75061 cgcggcttaa gttttccacg tcgtcagtta ccgggttaac gccgatcacc gcatcgccca
    75121 ccccgaagga aagcccttca tagatttgcg cagcgatact ttgcacgtcg tcacgggtat
    75181 cgttcggctg caaacgggcg ctaaaggtgc ccggaatacc gatggtggta ttggcctttt
    75241 tgattaccgg cattttcttc gcgccgtaga tcaggtccgc gttggagcaa atcttcgcta
    75301 tcgccgcgac cacttccgag gtcagccctt tgcgggtaaa ggcaatgtcg tccacgctgg
    75361 tttcatcgct cagcacatac tcacgcagtt cgctgatgct ccagttttta atctggttgt
    75421 aggccgtttc gttaacgtcg tcctgaatca gccgcgtcac gcagtcatct tcataggcaa
    75481 tcaccggatt attgcggatg tccgctacgg tcatttccga caacacctgc tttgccgcca
    75541 cgcgctcctg tgagcttgcc gctgcaacgc ccgccagcac atcccccgaa cgcagttcgt
    75601 tggctttagc cagcacctct tttacatcct taaactgata tacattgccg aacaatgtgg
    75661 tctttagttt cataagtcgt tccctcagga agaagtgatg agtaacattt ctcacagcct
    75721 gcaaacaatt ctatttcatc gttagccctt gtcatgtaag gtgatttcct taacacgccc
    75781 gttacttatc ctgccgaacg caaccgaact cgccggatac atgagcgggg ttatcgagtc
    75841 gttagatcga ggtatccggt tttgagcaaa acaccactca cagcaaaggc catagacgcc
    75901 gcacaaccaa aggacaagca atacaaactc accgcttcac tgacgcccgg cctgtttttg
    75961 ctggtgcatc ccaacggcag caaatactgg cgattccggt actggataga caagaaagag
    76021 cggttacagg ctattggggt atatccgctg attagcctca aggaagcccg caaacgcgcc
    76081 acagagagcc gtttactgat agcccaggga attgacccaa tggatttggc acgcaaggag
    76141 aaagccattg atgcactcaa catggcggca ggttttaaga cggtagcgga ggactggctg
    76201 gctaccaggg taggcggctg gagtgagagc tatacaaagc aggtcaaatc agcactggag
    76261 aaagacgttt atccggtact tggcaagcgt tcaatcgttg atataaccgc ccgtgacgtg
    76321 ctggcattac tccagaagaa agaacgtacc gcaccggagc aggcccgcaa gctacgccag
    76381 cgcatcggtg agatcttcaa gtttgccgtt atcaccgaac tggtaacgag gaatccggtt
    76441 gcagatcttg atacggcact gaaggcccgc cgacctggtc acaacgcgtg gataccgatt
    76501 agtgaaattc cggccttcta caaagccctt gagagggccg ggagcgtcca gattcaggcg
    76561 gcaatacgtt tgcttatatt aacggcatta aggaccgcag aactgcgttt gtgccgttgg
    76621 gaatggataa acctggagga cgcaacaatc acattgcccg ctgaagtcat gaaggcccgc
    76681 cgcccgcatg tggtcccgct atcacggcag gcggtcgagc tattgcagga ccagttcaca
    76741 cgcagcggat acagtgtttt cgtgttcccg ggccgattca tggataagcc attatcagcc
    76801 agcgcgatac tcaaggcgct tgagcgtatc ggatacaagt caatagccac aggccacggc
    76861 tggcgcacaa cgttcagcac cgcacttaac gaaagcggca ggtataaccc tgattggatt
    76921 gagatccaac tagcccacgt cccgaaggga attaggggag tttacaacca agcggcctat
    76981 ctcaaaatgc gccgtcaaat gcttcaggac tacgccgacg cgattgattc gatactggct
    77041 gatggtatca atccgcttga actgtaatga atgattaatc acgttttccg acgactccac
    77101 cggagtaggc cggataaaca ctggacacct tcaaaataac tattcacttt ttccggattc
    77161 gtccggcttt ttttgtcttt gccgatggga tcaattattc caattattcc agtatcgttg
    77221 gaataaatga ataaatatca atgacttaaa gccgtgtttg caacagggtg gatcggggat
    77281 cgaaaatagg atcaacacaa aaacaccctg gaggctacag cacactaggg ggacacaaaa
    77341 acaacacagg aaaaaaatac catgtaccaa tcagcaaccg cctacgtggc gaaaccaggc
    77401 gaaacactgg tcaaacacaa aattgcaaca ctccaagcac ttcaaacact ccaagccctt
    77461 ccggaacagg ttcaggccgc ctttcgtgat ggcatgaaca tcatccgctt cgatgggtat
    77521 cgctgggaat acaagccagc aactggattc gtgcgcatag atcaaatctt gtcgccagcc
    77581 aatgaagagc cagcgctagc caatgcagaa ccagcgcagg agcaggagca ggagcaggag
    77641 caggagcagg agcaggaaca ggaacaggaa caggaacagg aacaggaaca ggaacaggaa
    77701 caggaacaga ataaagcggc ggctattgcc gagttcaagg agctgcaaag acaacaaatc
    77761 acagcgcagc aagaagaatg ccgccacccg ctggcaatgc ccaagccagt agcgaaagcc
    77821 aacacagaca agccgacttc atggtttaac gaagaagcgg aagaagattt gcgttttgat
    77881 agtaaagagg agcagttgcc tgaagagcgg gaacaggtgg aaacggaaga ccagcaaaac
    77941 gattatcgca accttccagc cgtcaataac gctatggctt acctcttcaa aagcgggctg
    78001 tttcacaagc tgaaaggcga tgagttaaag ctatatacgt ggatggtatc caagtgcagg
    78061 tatcgcggta acgacaaata tgagatttgg tattcccgca ccaaaatggc aaaagcgtta
    78121 ggtaagagcg ttgacacgat agatcgccac ctggaagcgt tgagaaagct gggacttatc
    78181 caggaaatgc gccaggcact gaggggcaaa tgtggaaaaa cctacagact gcgaatcccg
    78241 tcaaaaggca cagcagaaga ggcctcaatg acggcacagc agcacgaaag agaagcaaaa
    78301 gtcagctacc tggtacacta ggcagcgggt cagcaaaggg gggcaaatgc cccttttttc
    78361 atgtcagata atcagatcat tacctcaata aactaataat cacatcgcgg ttacaaccag
    78421 tttgatagtg atagctcaaa cccagacgtg gcgctggctg caagcaaaat aagccgcaaa
    78481 aatgcggtcc tatttgccag aaccttttat tattcaattg gttagtgcaa gaaaaagcgt
    78541 gattactgtt caagaagcat ccagaaaggt catgattttg ccatgatttt gacgctgaaa
    78601 aaaaataggt gggcattttt gcccactcac tagccgcaaa tttgcccact cactagccgc
    78661 atttttgcgg cccaaagtaa taaatacaga gaaataataa atacagatca gcgccaagat
    78721 ttttcgtctt ttcggcggaa tcatgagact agtgcttacg tcctgataac ctccagagca
    78781 gaccttaact tctgtgcagc aggactacgc tcattgccgc tcggctaggt caaatcctca
    78841 cgataaagct gttcggattg tgaccataat gttaaaggac tctttaaatc attccttgac
    78901 gaattaacta gctggcttaa gcggaatagc atgcaggata aaaacaaaac cagcttagca
    78961 gtggggagag cttcgcttcg ctcgtctcct ggggccaatg ctcaatgaaa cgacgtaagg
    79021 cacggagcgc tcaccatagg tagacacata aacatctgag agcgcacctt ctatcgcgct
    79081 acgcgctcaa taaaaaccaa cgacaccgcc acactgcaag accaatggtt tttatcgcgt
    79141 atacagacca gacaggaaac agcagcaaag cagcgtatag ccaggtgcag acagcacact
    79201 cacacgacgc acagcaggct taagtgatca ctcaggaaac tttatccccg ccgccgatca
    79261 ccgcgaacta tcatgaccgc gccaaattcg aaaggaatgg cccatagaac gcgatgaaca
    79321 cacataagcg ctaacttgct tacctgaaaa cttaacgtcg ccacagagcg attgagagcg
    79381 cttaaaatcg atgcgatttc attaaatttt tctcactcgc ctgaatggtg gcgatcagga
    79441 ctgatcgcaa cattgccccg cctcgatatt gagcgcataa tagatacata cattaagcag
    79501 aaaagagata agtttattgt gaaaatcagg gggttacaca tcatgcgcat atcagaacaa
    79561 caacggcaga tcctcgacta tctggcaaaa aacgaatcaa tcaccgcacg acaagccgca
    79621 gatctcgttt atggtggcaa tgttacccgc gtccaggttg acgccgcccg ccgctcactc
    79681 atgacgatgg ttagtaatgg gctaatcagg aaacaaggcc gcgctttcgt agctgaaaga
    79741 catatcaatc acgcttatag ccaggacgtt gaagaattgc gccgccagat cctttgggta
    79801 ttcagcaaac agggtcagga gtatttcctt tctgaacacc caaatttcgc cgccaaagag
    79861 ttaacagtaa gcggattgtc gtgcgcactg gattatcttg tcggtgaagg ttacgttact
    79921 tacaagcagc tatggcacgc cctgaatacg cttgtagaag atggtttact cattgccacg
    79981 cgtgagaaca tcgaatactg gataatgccg cgttcaggtc gccgcaatca tgcacggtat
    80041 atttcaggca acagatacag aacgccggac tttaatccga cacaggaaga tcgggaccgt
    80101 gccgaaatga tgtgcaggga ataggagaaa agaattatgg ataagttatt ttcgatgcag
    80161 tgaacacaag cgggcgcaat gcccgttttt attgccttat tccacgcctg acgaatcaat
    80221 ttgatcacac ctgagcaaat gcatagccag aagttaaggc cgttatattg cgattgggag
    80281 cgttttaaac ggtatcaatt taattaattc tgatgtggtg atttgcaggc aaaaaaaacc
    80341 cgccggagaa gcgggaaata gagacatatc gttgtagtta ttaacagatc actttgaggt
    80401 cgagggcggc taaccctcgc taatattatc ccgccattac cttgcgggtg caaataatct
    80461 tttattgctt tgatgattta ataaatagca ctttttgcta acaaaaaccg ctgtcaatat
    80521 atggccaaaa atgtcaacag aaaaaataag gtttataata ttaagtatca catgggtaat
    80581 gggttcaggg aatcggaatc caccgcctca cggaaattca aaatttgctt cttctttcct
    80641 ccgtggggcg gcttcttttt cgtatcgagg tatttatgaa agcaataaca ttagaacagt
    80701 tcgcagcaat ggcgaatgta tcgaccagaa cgctgaggcg ctggctaaaa aaaccatcaa
    80761 ccggagtccc tttgccattt accccacgcg gtactcgcca attattctgg gagcacgaat
    80821 gcaaagaatg gttgtacaga acagaacgat aataatcacg catcacattt tgaggtaaac
    80881 atcatgacca atatcgaaag attagaacgc tgtgccgata ttatgcggcg cagatggatt
    80941 tatgaccctg aagcgggttt attgtttagc cgtgaaacaa aagacgttat caaaggcagc
    81001 atgaccaata aagggcattt ggttgtaacg gtacatgaag gtaaattcaa agtgacactc
    81061 acctatcaga aagcctgcta tgtatacaca tacggcgctt atgacgagac attatatgaa
    81121 gtgcaccacg ttaatttgga caaacaggat aaccgcatca agaatattcg cctgatgccg
    81181 aaagaaaagc accgccggat acataaaaac gtccgcaaac ttattcggct gggccacaca
    81241 caatggcttc aaccgcaatt aattgaacaa tcactataaa cacagtagta aataccatgc
    81301 attcattaga cagttatttt cagcgcacga cagcccctaa atcagccgcc caggaacgcc
    81361 gtgaggaatt ccatgagaaa gtgatgcgtt ctgctgatta tattgcggac aagttcgtgg
    81421 aaacggttcg tccacttgtt gatgaagtag ctgataaact tcagtctgaa atgccggaag
    81481 atatggaagg cactgcaaag cggcgactca tttgctaatt aagtcgtcgt tttggcgtgt
    81541 caataagcgc ttttaaataa tcgaaaaaag gggggaaaga taatggacgc atttgaggta
    81601 ctggcaaata aaggcgctga actggtagca cagcgtgata aaaccgccaa cgaaggggaa
    81661 cgcgcactgc taaataaaca gattaaagcg atccgtatgg cccagtttaa attaatcagt
    81721 aatgaagtta tcgaaacaat taaaccgcag atcccacaaa tcgttgctga cgctattaaa
    81781 gccgccggac tggagaaacg cattgccggg ctaaaaacga acgcagagaa aatggagatg
    81841 cacaaacaga gcggtgctcc gaatcccgat gagtacttct caccggatgt tgagtttatg
    81901 gcgcaggttg aggaacgtct gggtagctgt ctcaccgagg aacagcgtcg ctattttgat
    81961 ggagtggata gttcggcagg tattgacctg aactcgtatt tcggtcgtga aattgagcat
    82021 tttgatgccg ccccgtctat gccggagcca gaaccagaaa tggccaccga tcctgaagct
    82081 gagcaacgga aacgcgtatt caatgcgata gggattcggt actgatcgga taaaagaaag
    82141 ccgggaatta ccccggctga cttaacaatg tgaccaatga acaaatcgga caagacaaac
    82201 aggataagca gtctcgaaac aatgttcggg aactatatta gcacaagtcc ggagaaaatc
    82261 atcatgttgg cactacgcat catgcagggc atcgctaaga ccctcgcaga acatgtctta
    82321 gacctgaaac attcgccact gagcaaacag gccatgaagc gctaaacgct tcgtctgtgg
    82381 gctgaatatt cgcttggaac tatcaataaa atcatcgaca tgaaatcggg gccgagcaac
    82441 cagagcgcgg aggaaatgga gttcatcaag cgtttaattc tcattcgcag agacattcat
    82501 agccagttac acagcgtggg aatcgatatt aatgatggga cgggcgatta attttcggac
    82561 aagtactatg cgcgcgcgag gagctgaaca tgaagcaaga tattgcagac aggctggaga
    82621 tactcgaagg ccagcgggca gaagcgaaac aactacgtaa gcaggccaga agagcgcaca
    82681 ggaacaacga agcagagtta ctaaccaaat acatcagttt caccaattac tgcatctacg
    82741 agtgttacaa ggaagatgca gaggactggc tggattcatt gccagaacag tactgagcag
    82801 aataacacca cgtcatgttg ttctgaggtg agcatatcac ctctgttcag gtggccaaat
    82861 ttagtgtgcc acttcagcat caccgttgaa atcacaataa cgattttttt gtaaatatgc
    82921 tctcacatat gtaggaatag accaggttgg cattgaaaca catctaccat gaccataata
    82981 aagtaatttt ttgatatgtt tatggtttta tattaattaa aatatatcta aaattgatag
    83041 ttttaaaatt gcaaaatcca gattgtgttt gtaatgttta atctgattgt attgagtggc
    83101 gtttattatg aaaaataaat caggtaattc ttgttataga gaagcagtcg atattcatag
    83161 ctcacttgag ctttcattta tattgcataa atatcatttg cgtgtagatg agaagaaatc
    83221 tcgaaatgta ttcagcgcca tacgtgatga gctcatcctt gatggggatt cacgacaaaa
    83281 cttagctact ttctgtcaaa catggcttga tgatgaaatc cgtgagttgc ttaatttagc
    83341 tattgataaa aacatgattt ataaagatga atatccacaa atggcggaac ctgaaggtcg
    83401 ctgtgtgcgt atgctttccg attcgtggag tttccccgat tcgcgacaca ccttaggttg
    83461 ctcaaccatt ggttcatctg aagctgctat gctcggccgc ttggcgttaa aatggcagtg
    83521 gtgtaaaaaa cgcgaagtac aaggtaaatc aaccgaacct gatttgcgat cctgtgcaaa
    83581 tatgctggca taaattctgc cgttactgtg atgtgaaatt gtgtgaaatt ccacttgatg
    83641 atcatcacct gataatgaat gctaaagagg ttttgaagca tgtcgatgaa aacactattg
    83701 gtgtcgttac gactttaggt attacttatt tttgtaaagc agattgcgtt agtgttttta
    83761 agataagtgt taaagttaat attagttttt atatttacat cgtaattata tgtttctatt
    83821 ttaaaaatat tcgagagtat aaaaatgtct aatgctaata tcgcacccaa aagtaagaaa
    83881 atgacagtgg ggacactcgc tatcatgaat ataactgctg tagtaagtct acgatttata
    83941 ccttctgaag cagaatacgg cttaggtgcc attttttact atacctttgc tgcaattatg
    84001 tttttagtac ctgttgcctt tgttgcggct gagctagcta caacatatcc acaaaaaggg
    84061 ggggcatttc gatggatagg agaagcattt ggggctcgct ggggttttgt aggaatgctt
    84121 atgacctggc tacaagtcat cccctacttt ccaacagtac tgacatttgg tgcagtctct
    84181 gttgcattta ttgatgttga tatgggggtg gcagaatcaa ttgccagtaa taaatggtat
    84241 attttatttt tcgtactatt tgtttactgg ggagctattt gtgttgcatt acgtggcgtt
    84301 ggtattttct cgcaggtatc taagtggtgc ggtattatag ggacaattat tccagctatt
    84361 atccttgtta ttcttggttt ctcctatctg tggttcagcg gtaaaccagc ggtgatccca
    84421 ttaggttggg gtgatctttt ccctaatata gctaacttcc agaatattgt tcttgccgcc
    84481 agcatatttc ttgcttatgc agggatggaa atgaatgcgg tccatgttaa cgatttagat
    84541 aatccaacta aaaaataccc aattgcaatt accatcgctt ctataggtac tattgcaata
    84601 ttcctgctca gtactttagg ggtcgccttt attattccta cagataaaat tagtctaact
    84661 caaagtttgt tggttgccta cgatatgctg ttcgaatggg ctggagtgcc atggcttggc
    84721 tctgtgatgg catttatgct agcaattggc gtactgggtg gggtggttac ttggattgca
    84781 gggcctaata caggtgtatt agcgatcgcg aaagctggtt atttacctaa gtttttccaa
    84841 aaaaccaatc gtcatggaat ggggcaccat ctaatgtttg tacaaggtat aattgtctca
    84901 gttctttctg ttacttttgt gataatgcct tcagttcaag cagcttttca gattttatca
    84961 cagttaacag ttatgctcta tttagttatg tatatgctca tgttcgctag tgctatctat
    85021 ctacgacatt ctcaacctga tacaaaacgc ccatatcgag cccctgcttt atttttctgg
    85081 gcaacacttg gattcttagg gtcttttctg gcttttgtat taagttttat tccgcccgag
    85141 caaatcccaa ctggtagtcc aaccagctat gttctttatt tgttagtatt agttgcccta
    85201 tttttattaa ttccaatatt aatttacgcc ttccgaaaat ctgactggaa agatccaaac
    85261 tctgatttcg aaccattttc ttgggaaaaa agaactagta attaaatttt ccataagcta
    85321 caaaagatga ggttaaatat gtttagtgct gaatctccaa ttggtgtaat agactcaggt
    85381 gtaggtggat tgacatcggt aaaggaaata attaatttgc ttcctggtga agatattatc
    85441 tattgtggag ataatatgaa tgcaccttat ggcaaccgtt ctgccgatga tatcatcagt
    85501 ttgactaaaa aaatgctgac ttttttacag tcaaggaatg tcaaactagt tgctgtcgca
    85561 tgtaatacta tatcctcaac tttggaatcc gaaaaataca gcggatatgc taagagcttt
    85621 ccgtttccta tcttatccat cattgaacct gcagtagaag atgtgattcg ccagcaatat
    85681 aaaaatgttg gtattatcgc taccgagttt actattaaaa cggggtgtca caaagagcta
    85741 ataaaaaaat tgaattcaac aataaatgta tttggagaac caagcaaaaa tcttgcaatg
    85801 ttaattgagg aaggtaatct taatgctcct gcaatcttga atgacatcaa aaaacatgtt
    85861 aaccatttaa tttccctcca tccagtaaat gaaattattc tcggctgtac tcactatcca
    85921 attgttcaga atttttttga agcagttgca ccggatataa aatttattaa tcctgcacat
    85981 gatcaggcta tctctattaa aaatcacttg ggacagctaa atttattaaa ctcctcaaat
    86041 ataggaactt tgcaaataaa tactagtgga agtatggaga tctacaaaac ggtactaggg
    86101 gaattgagca ttacgaaacc tcatacattc agtatcaggc agttttaaat ttatccggtg
    86161 caccgtaaaa tcccgtcctt ctgaactgca ccccaaaagt tggacaccaa ctgagtaagg
    86221 tgcattttat ggacaaaccc agttacatac tgggtagtgc atccaattag tagaacatgt
    86281 gtttttcgat aaacgctccg atcacttttt cgtggatctc aactgagcgt gagaagcaga
    86341 ttgttttacg acccaaccgc ttaatgcggg tgcgtagcgt cagattatta cgctcaatgc
    86401 gttgggtgaa tattttgccg gtcagatgct tattcttcgg cacctcccgg ccatagctgc
    86461 cccagtcatc gctggtcagc atgccgatgt tgaagggtgg cagtgccagt agctcccggc
    86521 acgtttgatc ggttcgggga ccaaaagtgt aggccagtac accgcctgtt ttggtgttgt
    86581 acgcgtaccc gagccaatgt tgccgggctt tactgccaac gtagctccat tgctcatcaa
    86641 gctcgcagat aaacgccaca tcagcatggg caacaggcga agaagttatt cgctttggcg
    86701 cgagtttttt aaagtccgga tgacggtgtt actaccaatt ttcagtgtcc tggcggtatc
    86761 gcgaaccccg gcaccattaa aggccatttc agttatcagc tctttcatac ccggcttacg
    86821 tgcttgataa gtataagtga actgaaacac acggtggcag tcacggcagc gaagtctgtc
    86881 acggccttta gggttctgac catggcggta aacctgagct gactgacaac ggggacaatg
    86941 aatattaacg ctggtcatga gataacctca aaagcccgta ttatacatca gattcaacta
    87001 attagaggca tcacccatcc agcattataa aacatactaa caataactaa attataataa
    87061 tcacaactta caatcaaata ataaatgcat aagattaaca atgatttttt cgggttatat
    87121 ttatctaaaa aagcagaata ttatgtaact tattattttc taagcgaaaa tttattgtgc
    87181 agtcagacta gcaaacttgc gtaccacccg aagaacatgc gaagatttaa gtattctttc
    87241 ttttttggtt tttatttttc attttctctt ttcttctctt tccggttctc ttctgttctg
    87301 gttttggttt tttccttttt atttttattc tttcttcagg gttatgctaa aacaccaaaa
    87361 catttttcac cacaaaagca tccaatcatc ttgccagacc aatcacctta accggataca
    87421 ctgccggata taatcccact aacacccagc caccaagcac catacctgat aactaccgta
    87481 ttcaggaagg aaatgcgagt gatttcaccg tcaccggcac aaccgatccg ccaaaaagag
    87541 gcgtaccaat gtcgatatag tcccccgcgc ggacaatcac ttcgtcaatg actgccaacg
    87601 ggagttgttg tagctgtggg cgcaacaaca tgcccagagc tttaccaaag tcctgcccgg
    87661 ccaccaccag caggggatgc ggattcggaa aacgcgcgac gaaatcgacc agcgcgttga
    87721 tgaccgtcag taccgcggcg taacgcacag gcagcgaggc gggaagcgcc agcacgtaca
    87781 cgtcagtttt gggatcaaga tccagctgaa gcagcgcctg ttgccaggca ctcaccagat
    87841 ccgtttcatc aatcgggatc gccaccggca aattgcgcag cggcagttgt acgccctcca
    87901 gccagattgt gctgccagag agcgaaaggg tatgtgcacc cgcgccaatc accgtggcgc
    87961 atacggtttg cgccggaaac tgcacattca tctcacgcag gcgcggatgg tcatgcagcg
    88021 ccgttgccag aagtgggcca atatcggcaa aacagaatgg gtcggcgggc tggtggcgat
    88081 aacattcgcc cacacctcca gaaagcgtaa tgatttcggg cgtaacacct gcgggcagca
    88141 aaccggtttg catcaatgcc tgcgcgagcg gcgaaagcgt tccgtcaatc acttcgacaa
    88201 tcagcgccgc catccgccgg gtaacctgca ccagctgcgc gccggtcagc gaacgggcgt
    88261 cagtgcctgc accgaagcac tcatccacaa tcatctgccc cggtttatga gcgtaaacca
    88321 cgcgcccctg gctgtcggtt tccagcaggc gaccaccgac gttgagacag gcagtgccgc
    88381 tgatttttcc ggcatcgaac agggcgtagt tcgcggtgcc accgccgata tcgatattca
    88441 gtacccgaca cagccgttgt tcagaaaggg tttgcgcccc ggctccgtga ccggcgatca
    88501 cggattcgag gtgcggcccg gcgctggcga cgacaaaatc gcccagcgac tgtttttcct
    88561 tcatcacttt ttccatcagc tgtgcgacca ggctttcggt aaactggcct tccggcagct
    88621 gggcaatgat ggtttcgcgg atgcgctggc tttcggtttt gtcggctggc acgctggcgg
    88681 gcacggacgc cgttaccggg atcgattcat cacattcggt gatggtaaaa cccagtagat
    88741 ccgacacttc acgggcttcc ggggtaataa tgctggcgcg cagaaccacg gacattgcct
    88801 gttcgccgcg cgcgtgtgct tcacgaatat cattcgctgt gatgagtttt ttcacctgct
    88861 tcctcctgtt tggtgagaga tgccagataa tccaccagtt gctgcacgct ttgcgggtcg
    88921 tggctgttta gctcgaaaat cggctcttcg aaacccgtct caagcaataa ctttcgcgtt
    88981 gcggcgacgt cagcatctgg catgtccgtt ttactgatga cggcgatttg ccgtttgctg
    89041 acgccaatat ccagcaaccc ggcaggcaac cgactttccg ggtcatttgc gccatggaca
    89101 tagatcagca tgtctacatc ctgtagcgtg gtaattaagg cgtgatacca gcggggatgg
    89161 ctaaaatatt cacccggcgt atcaatatcg cccttatcat taaattccac ggcctgtgtt
    89221 tttctggcga gggtataatt cccctgcaac gcattaaata acgttgtttt tcctgccccg
    89281 acgctaccca caaacgcaat tcgtttcatg aatgcctcgt attaactttt ggtcatttca
    89341 caaagcgtat aatttaataa gcgacctaaa ccgctgacgg tttgtgataa cgcttcttct
    89401 actgcgccca ccgagccata aatcaccaat gcaccgctga agcggtcgag aaaaccgata
    89461 tgcacatcag cggctttcag cgctaaatca ccggcgatca tcgccgtttc gccgggagtt
    89521 agcgtcataa tgccaattgc gcccgcatcc ggaacgccga ttttcttcgc cagttcctca
    89581 ccgggatgcg cgatgagatg cgccagtgtg acctgtttgc cgggcacaaa ttcctgaatg
    89641 atgcgttctt tatccatgag tcgccgcctg tgaaaaactt tcgtgtatcg tgacaatttt
    89701 tcgtcagagt aaataacaaa attcggcaag cgcgtttaaa aggtgaggta gatcactaag
    89761 aatggagaga tatttactgt tatgaataaa ctttcatatg cgacgcatga atataaataa
    89821 aataatatcg agatatttta tatactgatt tttttattat tcgcaaactc agtaataagc
    89881 tttgctaata tatttcagga aaaccgctta ccctgaatat tcagggtaag cgtgagagtt
    89941 aagaatgaat tacagcggtt gggtttgcgc ttcaaccacg gccagcgcca ccatattgac
    90001 gatacgacgc accgatgcga tcggcgttaa cacgtgaacc ggtttcgcca cgcccatcag
    90061 caccggaccg acagtcacac cttccgagct ggaaacgcgc agtaagttgt aactaatgcg
    90121 ggcagcttcc atgttcggca tcaccagaat attggcagaa cctttcaaag ggctgtccgg
    90181 catacggtcg ttgcgaatcg cttccaccag cgctgcatcg ccgtgcattt caccatcaat
    90241 catcagatct ggcgcacgat ccctgaccag ttccagcgcc tgacgcattt tgctcgacga
    90301 cgggcagtca gaagaaccaa agttggagtg cgacaacaga gcaacgcgcg gctcaatacc
    90361 aaaacgacgg acagtttctg ccgccatcaa ggtgatctcc gccagctctt ctgcgtctgg
    90421 ttcgtcattg acgtaggtat cggcaacaaa ggtgttacca ctcggcagca gcagcgcgtt
    90481 cattgcacct gcggtgtgaa cgccatcgcg ataaccaaag acatttttca ccacgctaaa
    90541 atgttcatga taatcgccca ccgtaccgca gatcattgca tcggcttccc cacgctgaac
    90601 catgatcgcg ccgatcactg tcgggttact gatcagcgca cgctgcgcct gttcctgagt
    90661 gacaccgcga cgcttcatga tctggaagta ttcggtccag tactctttaa agcgcggatc
    90721 ggattcgtta ttgacgattt caaaatcaac gcccgctttg atctgcaagc ccagtttctg
    90781 aatgcgcatt tcgatcacgt tcggacgacc gataaggatc ggtttcgcca gtcccagcgt
    90841 taccagttcc tgagtcgcat gcagaacgcg cgcctcttcc ccttccggca gaactacgcg
    90901 cttcggcgct ttgcgagcct gggagaaaat cggcttcata aacaggttgg ttttgtaaac
    90961 gaactcagtc agcttgtcga tgtagacgtc gaaatcagca atcggacgcg tcgccacgcc
    91021 cgactccatc gccgcttttg cgaccgcagg agcgatctta acgatcaagc gcggatcaaa
    91081 cggttttgga atgatgtatt ccggaccaaa gctcagatcc tgaacgccat acgctgaagc
    91141 caccacttca ctctgttctg catgggccag ttctgcaatc gcgcgtaccg ccgccagttt
    91201 catctcttcg ttgatggcgg ttgcgccaac gtcaagcgcg ccacggaaga tgaacgggaa
    91261 gcaaagaacg ttgttcacct ggttcgggta gtcggaacgg ccggtacaga tgatggcatc
    91321 cggacgcact tctttcgcca gcggcggcag aatttccggt tccgggttcg ccagcgccag
    91381 gatcattggt gcccgagcca ttttcttcac catttcctgg gtcagcactt tcgggccgga
    91441 acagcccagg aaaatatccg caccttcaat cacatcatcg agggtacgtt tgccgtcatc
    91501 caccaccgca tatgcggctt tggtttccgc catatttggc tcacgcccct gatagataac
    91561 gccttttgaa tcgcaaacca cgatgttatg tttttgcagc cccagcgcta ccagcaggtt
    91621 catacaggcg attgctgcgg cacccgcgcc ggaaaccacc atccgcacgt cggagatgtt
    91681 tttctccacc acgcgcaagc cgttgaggat ggcggcagtg ctgatgattg ccgtgccgtg
    91741 ctgatcgtcg tggaataccg gaatattcat ccgctcgcgc agtttctgtt caatatagaa
    91801 acattctggc gctttaatgt cttcgaggtt gatgccgccg aaggtcggtt cgagcgcggc
    91861 gacgacttca ataaatttgt ccgggtcgag ttcatcaact tcaatgtcaa atacatcaat
    91921 cccagcgaat ttcttaaata gaacgccctt gccttccatc accggtttgc ctgccagcgc
    91981 gccaatgttg cctaacccca gcaccgccgt accgttagag atcaccgcaa ccaggttacc
    92041 gcgcgcggta tatttgtagg cttttagcgg gtctttttcg atttcaagac aaggtgcggc
    92101 aacgcctggt gagtaggcca gcgccagatc gcgctgcgtt gccagaggct tggttggaga
    92161 aacctggatt ttccctggaa ctggaaattc atggaaatca agtgcacttt gttttaactg
    92221 gtcatccatt tggttgttcc tttcacgtaa cgttcacaaa taaagtgtgt gggcaacagc
    92281 ccctgcccac aacgtggcgc acattattac cctgccggag tctacagact ttgagcaagt
    92341 ccaaactctc accattaata taatgttttg gtaacaatcc tataacactg atattacctg
    92401 cttaatccag caataccatg cctgtctgct atgctttttt gatgcgttta gcgaaatttc
    92461 tcagaagtgt gaattaacgc actcatctaa cactttactt ttcaaggagt atttcctatg
    92521 aacgagttag acggcatcaa acagttcacc actgtcgtgg cagacagcgg cgatattgag
    92581 tccattcgcc attatcatcc cctggatgcc accaccaatc cttcgctgtt actcaaggct
    92641 gccggattat cacaatatga gcatttaata gacgatgcta tcgcctgggg taaaaaaaat
    92701 ggcaagaccc aggaacaaca ggtggtcgca gcgtgtgaca aactggcggt caatttcggt
    92761 gctgaaattc tcaaaatcgt acccggttgc gtgtcaacag aagttgatgc acgcctctct
    92821 tttgataaag aaaagagtat tgagaaggcg cgccatctgg tggacttgta tcagcaacaa
    92881 ggcgttgaga aatcacgcat tttgatcaaa ctggcttcga cctgggaagg aattcgcgcg
    92941 gccgaagagc tggaaaaaga aggtattaac tgcaacctga cgctgctgtt ttcttttgcc
    93001 caggcgcggg cctgtgcgga agcaggcgtt tttctgattt cgccgtttgt agggcgtatt
    93061 tatgactggt atcaggcacg caagccgatg gacccgtatg tggtggaaga agatccgggc
    93121 gttaaatcgg tgcgcaatat ctacgactac tataagcaac accactatga aaccattgtg
    93181 atgggcgcga gcttccgtcg caccgaacaa atcctcgcct taaccggctg cgatcgactg
    93241 actatcgcac cgaatttgct gaaggagctg caggaaaaag tttctccagt ggtacgtaaa
    93301 ttaatcccgc cttctcagac gttcccacgc ccagcaccca tgagcgaagc ggagttccgt
    93361 tgggagcaca atcaggatgc gatggcggta gaaaaactgt ctgaaggcat tcgtctgttc
    93421 gctgttgatc aacgcaaact ggaagatctt cttgccgcca aactataaac cagccacgga
    93481 gtgttatatg tcccgaaaag accttgccaa tgcgattcgc gcactcagta tggatgcggt
    93541 acaaaaagcc aattctggtc atcccggcgc gccgatgggc atggctgata ttgccgaagt
    93601 gctgtggaac gattttctta aacataatcc taccgaccca acctggtatg atcgcgaccg
    93661 ctttattctt tccaacggtc acgcgtcgat gctgctctac agtttgctac atctgaccgg
    93721 ttacgacctg ccgctggaag aactgaagaa cttccgtcag ttgcattcga aaaccccagg
    93781 ccacccggag attggctata cgccaggcgt tgaaaccacc accggcccgc ttggacaagg
    93841 tttggcgaac gccgtcgggc tggcgatagc agagcgtaca ctggcggcgc agtttaacca
    93901 gccagaccat gagatcgtcg atcacttcac ctatgtgttt atgggcgacg gctgcctgat
    93961 ggaaggtatt tcccacgaag tctgttcgct ggcaggcacg ctgggactag gcaagctgat
    94021 tggtttttac gatcacaacg gtatttccat cgacggtgaa acagaaggct ggtttaccga
    94081 cgatacggca aaacgttttg aagcctatca ctggcatgtg atccatgaaa tcgacggtca
    94141 cgatccgcag gcggtgaagg aagcgatcct tgaagcgcaa agcgtgaaag ataagccgtc
    94201 gctgattatc tgccgtacgg tgattggctt tggttcgccg aataaagcag gtaaggaaga
    94261 ggcgcacggc gcaccactgg gggaagaaga agtggcgctg gcacggcaaa aactgggctg
    94321 gcaccatccg ccatttgaga tccctaaaga gatttatcac gcctgggatg cccgtgaaaa
    94381 aggcgaaaaa gcgcagcaga gctggaatga gaagtttgcc gcctataaaa aggctcatcc
    94441 gcaactggca gaagagttta cccgacggat gagcggtggt ttaccgaagg actgggagaa
    94501 aacgactcag aaatatatca atgagttaca ggcaaatccg gcgaaaatcg ctacccgtaa
    94561 ggcttcgcaa aatacgctta acgcttacgg gccgatgctg cctgagttgc tcggcggttc
    94621 ggcggatctg gctcccagca acctgaccat ctggaaaggt tctgtttcgc tgaaggaaga
    94681 tccggcgggc aactacattc actacggggt gcgtgaattt ggcatgaccg ctatcgccaa
    94741 cggcatcgcg caccacggcg gctttgtgcc gtataccgcg acgttcctga tgtttgttga
    94801 atacgcccgt aacgccgcgc ggatggcggc actgatgaaa gcgcggcaga ttatggttta
    94861 tacccacgac tcaattggcc tgggcgaaga tggtccgacg caccaggctg ttgagcaact
    94921 ggccagcctg cgcttaacgc caaatttcag cacctggcga ccgtgcgatc aggtggaagc
    94981 ggcggtgggc tggaagctgg cggttgagcg ccacaacgga ccgacggcac tgatcctctc
    95041 aaggcagaat ctggcccagg tggaacgtac gccggatcag gttaaagaga ttgctcgtgg
    95101 cggttatgtg ctgaaagaca gcggcggtaa gccagatatt attctgattg ccaccggttc
    95161 agagatggaa attaccctgc aagcggcaga gaaattagca ggagaaggtc gcaatgtacg
    95221 cgtagtttcc ctgccctcga ccgatatttt cgacgcccag gatgaggaat atcgggagtc
    95281 ggtgttgcct tctaacgttg cggctcgcgt ggcggtggaa gcaggtattg ccgattactg
    95341 gtacaagtat gttggtctga aaggggcaat tgtcgggatg acgggttacg gggaatctgc
    95401 tccggcggat aagctgttcc cgttctttgg ctttaccgcc gagaatattg tggcaaaagc
    95461 gcataaggtg ctgggagtga aaggtgcctg atggtgatcg ccggatgctg tatgttcccc
    95521 tcaccctaac cctctcccca gaagggcgag gggacggtcc gtgcgctttt tagggccgca
    95581 tctgcgattt tgtttaacgg gtgatcacaa cgtgggcctg gcgtctggcc catgccagtt
    95641 atcgcaggta ggttctgcgg cgtaacgcac caggcgaaaa ctctgaccgt caaagcgcca
    95701 gcgcgcctga atgccacaat cgcttaatcc gcgccctttc gctaaggtca ccagttcacg
    95761 cgatttctca tcaaatgttg cgttcatcag ttccagttca ttcgtctcct gaccgttgtt
    95821 gaacggcaaa cgcaaccgaa ccgggcgcga ggctagcggc tttttacgcg acacaatcca
    95881 tgccaaatca atggtgttat aggcccctgc ctcacagcta atcatcatca gtgctttatc
    95941 atcggtcagc gcagtgacat tcacctcacg acgtaacgga tcaagcgagc agcgcagacc
    96001 attcatccgc cagtttccat aatccagcaa atcgttacgt tcttcgagtg agagtggcgt
    96061 cggcgttggg ttaaccaccg cgacctcttt cagcgcaggc gcgggcggta cgctgagagg
    96121 cggttcgtcc cctttcttga tccacgcggt ttcactgcca acgcgctttt gctgagcatc
    96181 aataaacaac aacgccgctt ttaagccgct cagagaaatg gtctgattgc cgtcgcgtaa
    96241 ggtgatcgcc ttcccttcct gaattatctg caaaaacgcg gtgatggttg ccgtatcatc
    96301 ggttactaat aaccatggtg aaatccgcca cttgtcacca cttaatgcca acggctcacc
    96361 atctaatagc agccgtggcg ctatctcccc ttcagacgca tccggcgact tcaacccacc
    96421 gcgctcaata cgtaaaacgg catcggtatg cgccccagcg ctgcggctca gggtcatcac
    96481 cagtccatta tgatcgcccg tattgcgcgc cacacaaaaa ttttgattat tacaggtgac
    96541 ctgccagtcg gaaaacgccc gttgcgctgg tgccgcccac accaaagacg tcggcaacaa
    96601 agcgaaaaaa aagagaagga aaatgcgata gcgcatggac ggtacgaccc cagaagttca
    96661 aacaaacagc acaagtcgta tcttcgttgc gcaggtagcg cggctcaatc ggatttgtcg
    96721 gattgagcca ataataaaca acgaaatatt attgctattt ttcagtccat taaatgtgac
    96781 atttgcaaat agttaagcaa taacaccgtc ttaccgtcac gtatctcgcc ggttttgatc
    96841 atctccagcg cctggctgaa cggcagctcg agcacttcaa tatcttcatc ttcaacaccg
    96901 ccaccggcgt tagcgcgctg attgtcactg tattcggcga taaaaaagtg gattagctca
    96961 gtcacaccgc caggcgacat atacagttca aataatttgc gcacttcgcc cacttcatag
    97021 cccgtctctt caatcgcttc tttacgaatg cacacttccg gttcgtcgtt atccagcagc
    97081 ccggcgcagg tttcaatcag ctgcccgctt tcattgccat taacccaggt agcgacacgg
    97141 aactgacgaa tcagaaccac gctctttttc ttcgcgttgt acaggaggat cgtcgcacca
    97201 ttaccgcgat cgtaaacttc acgtttatgg cggataactt caccatcttt gcgcgtgaga
    97261 tcgtaagtaa tgttgtgcag ggtgaaatag ttatcggaga gaattttgtc tttaatgagg
    97321 gtgatttgtt gcgtcatacc gactccacag cgcgaaatga acaattatat tacgctgtga
    97381 agtcgggttt gtctgcgcag gctatcagtg agatttgact gattttaccc ccagccagtc
    97441 aataatcccc tgtgccgcat gacgtccttc tgccattgcg gtaaccacca gatccgcacc
    97501 acgcacggcg tcgccaccgg cgaagatttt cggattggtg gtctggtaac ggtactggct
    97561 ttccacatcc gcgatgatgc ggccccattt gtctaccgtt acaccgtgcg actccagcca
    97621 cggcatcccg tgtggattga agccaaacgc cataatcacc gcatcggctg gcatgacaaa
    97681 ttcactgccc tcaatcggta caggtcgccg acgcccctgg gcatccggct cgccaagacg
    97741 cgtgcgcagg aaacgaattc cgcaaacgtg accttgctca ttcagctcaa gcgccaccgg
    97801 ctggacgtta aattcgaagt tggccccctc ttcgcgggcg ttcttcacct ctttcttcga
    97861 gcctggcatg ttagcttcat cacgacgata agcgcaggtg acgttactcg cgccgtggcg
    97921 cagcgcagta cgcacacagt ccatcgcggt gtcgccgccg cccagtacca cgacgttaag
    97981 tccggcggta ttgataaacg gctcttccgg tagctcttcg agccccatca cctgtttagt
    98041 gttggcaatg atgaacggca gcgcgtcata aacgcccggc gtatcttcat tgggtaaacc
    98101 cgctttcatg gaacggtaag tgcctacgcc aacgaagacc gcgtcgtatt gttccaaaag
    98161 cgaatccaaa gagacatctt tacccacttc acaattgagt tcgaagtgaa tccccatcgc
    98221 gctgaagatt tcccgacggc gtgccagcag ggatttatcc agtttgaaag aaggaatgcc
    98281 gaaagtgagc aagccaccga tttctggatg gcgatcgtac accgtcaccg ccacgccatt
    98341 gcgggtcaga acatccgcac aggccagccc tgccggacct gccccgataa tcgccacccg
    98401 cttgtccact ttggtgacat gacttaagtc aggacgccaa cctttcgcca acgcctgatc
    98461 tgaaatatag cgttcaatgt tgccgatagt taccgcgccg tgctcatcgc gaatagtaca
    98521 ggcaccttca cacaaacggt cttgcggaca aacgcgtccg gtaatttccg gcagggtgtt
    98581 ggtctggtga gaaagctcga cggcggcgtc gatgtttccg gctttcacca gttcaatcca
    98641 ctgcggaata tggttatgca gcgggcaggt ccattcacaa acgctgtgct cgccgaactt
    98701 aaggcagcgc gaggcttccc gttgtgcctg gtcggcgcga aatggcagat aaatttcatc
    98761 aaaaccggtt ttgcgcgctt caatcgccag tttatccggc tcgccacgcg cgggcgttgc
    98821 ctgcatttgt tcgactttac tcattaccgg catttcttgt gcagcggtac tggcatgcca
    98881 cggttgatgc tcctgacgtg cggtgcgcaa gcggcgggat ttcgccatgc cggagagtgc
    98941 gacgtcagtg accagttgca gcgcgtccgc cgggcaattc tcgacacagg caggaccgtt
    99001 ttcgcgccct gcacaaaggt cgcatttatg cgccgtggct tttacttttc ctgccgcgac
    99061 gggtgtcagg acgatttgca tcgtaccaaa cggacaggcc accacgcagg atttacagcc
    99121 aatacacttt tgctgattga cctgaatgct gtcatcaacg tggctgattg cgccattagg
    99181 gcagctacgg gcgcagggcg catcttcaca atggtgacag gtcactgcac tacgttgctg
    99241 ctgatgtttg ataaccgtaa ttcggggatg aaaatggtgt tggctcagga catgttgctc
    99301 atcattgtga gccatgacac aggcgatttc acaagcatga caacccagac attgctgact
    99361 gttggccata ataaaacgat tcataacgac cttctttttt ggttgtaaaa accttattct
    99421 ttatatgagt gttgttatta cccgacttac aggggaaccg gcaatgttca tgtgcccaga
    99481 ataagtaact atttcgctat aaactgtggc agatcaaata atcccatcac tgactaaatt
    99541 gcgtttcagc gaactggaac attaatgatt ttttgtggag aagacgcgtg tgattgttaa
    99601 acgccccgtc tcggccagtc tggcccgggc ctttttttac attgtgctgc tgtcgattct
    99661 ttccacgggt atcgctctgc taactctggc gagcagtttg cgcgacgctg aggctatcaa
    99721 tattgccgga tcgctgcgta tgcagagtta ccgcctgggc tacgacttgc aaagtggcag
    99781 tccacaactc aatgcacatc gccagttgtt tcagcaggca ctgcattcac cggtattaac
    99841 caacctcaac gtctggtatg tgccagaagc agtaaaaact cgctatgcgc atctgaatgc
    99901 caactggctg gagatgaata atcggctcag caagggcgat ttgccgtggt atcaggccaa
    99961 tattaataat tatgttaatc agatagacct gttcgtgctg gctttacagc actacgctga
   100021 acgcaaaatg ctgctggtgg tggcgatttc cctggctggc ggtatcggta ttttcacgct
   100081 ggtctttttt actttgcgcc gcatacgcca tcaggtggtt gccccgctga atcagctggt
   100141 taccgccagt cagcgtattg aacacgggca gttcgactcg ccgccgctgg ataccagtct
   100201 gccgaatgag cttggtctgc ttgcaaaaac ctttaaccaa atgtcgagcg agctgcataa
   100261 attgtacctt tcgctggagg cgtcagtaga agaaaagacc cgcgatctcc acgaggccaa
   100321 gcgtcgtctg gaggtgttgt atcagtgcag tgttcccagg cgctgaacac cagccagatt
   100381 gatgtgcatt gtttccgcca tattttgcag attgttcgcg acaatgaagc agctgaatat
   100441 ctggagctga acgttgggga taactggcgg attagcgaag ggaagccaaa ccctgagcag
   100501 ccaatgcaga ttttaccggt gacaatgcaa gagacggttt acggcgaact gcactggcaa
   100561 aatagtcacg tttcatcatc agaaccgctg cttaacagtg tttcatcgat gctgggacgc
   100621 ggtttgtact ttaatcaggc gcagaagcat tttcagcagt tattgttgat ggaagaacgt
   100681 gcgaccatcg cccgcgaatt gcacgactcg ctggctcagg tgctttctta cttacgtatc
   100741 cagttgacgt tactgaagcg ttcgataccg gaagataacg ccaccgcaca aagtatcatg
   100801 gccgattttt cccaggcgtt gaatgatgct tatcggcagt tacgcgagct gttgactact
   100861 ttccgcctga cgctgcagca ggcggatctc ccctccgcgt tgagggaaat gctggatacg
   100921 ttacaaaatc aaaccagcgc taaactgacc ctcgactgcc gtctgccaac cctggcactg
   100981 gatgcgcaaa tgcaggtgca tttgttgcaa attattcgcg aagcggtgct gaatgcgatg
   101041 aagcacgcca acgccagcga aatcgccatc agttgcgtca ccgcgccgga cggcaatcac
   101101 acggtttata tccgtgataa cgggattggt atcggtgaac cgaaagaacc cgaaggtcat
   101161 tatggactga atatcatgcg cgaacgcgcg gaacggctgg gcgggacgct gactttttca
   101221 caaccttccg gcggcggcac gttagtgagt attagctttc gctccgcaga gggtgagggg
   101281 aaggggaagg ggaacacgcc ggctgaggtg taaataatta ccctcacacc ggccctctcc
   101341 ctttaaaggc gagagggaaa aacgtgccca tattgacctg taagcgagtg tgtcagcaat
   101401 ctcatgataa tttacattaa ctcctttttt tctccacgat tggctcgtac cttgccgcta
   101461 cagtgaagca agtcaagcct acaacaatac gcagaaacac gaggtcctct tttaatggcg
   101521 aatttcttta ttgatcgccc catttttgcc tgggtgctgg caatcctgtt gtgtctgaca
   101581 ggtaccctgg cgattttttc attgcccgtt gaacaatacc ccgatctcgc gccaccgaat
   101641 gtgcgagtga ccgctaacta tcccggcgca tcggcccaga cgctggaaaa caccgtgacc
   101701 caggttatcg agcaaaatat gaccggcctc gataatctga tgtatatgtc atctcagagc
   101761 agtggcaccg gtcaggcatc tgtcacttta agttttaaag caggcaccga tccggacgaa
   101821 gccgtgcagc aagtacaaaa ccagctgcaa tcagccatgc gaaagttacc gcaggcggtg
   101881 caaaatcagg gcgtgacggt gcgtaaaacc ggcgatacca acattctgac cattgccttc
   101941 gtctctaccg atggctcgat ggataaacag gatattgctg attatgttgc cagtaatatt
   102001 caggacccgt taagccgcgt gaatggcgtc ggggatatcg atgcctatgg ttcgcaatat
   102061 tccatgcgta tctggctgga cccggcgaaa ctcaacagtt tccagatgac ggctaaagat
   102121 gtcactgatg ccattgagtc acagaacgcg cagattgcgg ttgggcaact tggtggtaca
   102181 ccttccgtcg ataagcaggc gctcaacgcc gccattaacg cccagtcact gctgcaaaca
   102241 ccagaacagt tccgcgatat caccttgcgg gtgaatcagg acggctcaga ggtaaggctg
   102301 ggcgatgtcg ccaccgtcga aatgggggcg gagaaatacg attatcttag ccgcttcaat
   102361 ggtaagccag cctccgggtt gggggtaaaa ctggcctccg gcgcaaacga aatggcgaca
   102421 gcggagctgg tgctcaatcg tctcgacgag ctggcgcagt atttcccgca cggactggaa
   102481 tacaaggtgg cgtatgaaac cacctcgttt gttaaagcct ccattgaaga cgtggtgaaa
   102541 acgctgctgg aagctatcgc tctggttttc ctcgttatgt atctgttcct gcaaaacttc
   102601 cgcgccacgc tgatacccac tatcgccgtg ccggtggtgt tgatgggaac cttctccgta
   102661 ctttacgcct tcggttacag cgtcaacacc ttaaccatgt tcgcgatggt gctggcgatc
   102721 ggtctgctgg tggatgacgc catcgtggtg gtggaaaacg tcgaacgtat tatgagtgag
   102781 gaaggactca ctcctcgcga agccacacgt aaatcgatgg ggcagatcca gggggcactg
   102841 gtcgggattg cgatggtgct ttcggcggta tttgtaccaa tggccttctt cggcggcacc
   102901 accggtgcca tctatcgcca gttctctatt accattgttg cggcgatggt gctgtcagta
   102961 ctggtagcga tgatcctcac tccggctctg tgtgccacgc tacttaagcc actgaaaaaa
   103021 gctgagcatc acgggcaaaa aggttttttt gcctggttta accagatgtt taaccgcaac
   103081 gccgaacgct acgaaaaagg ggtggcgaaa attctccacc gtagcctgcg ctggattgtg
   103141 atttatgtcc tgctgcttgg cggcatggtg ttcctgttcc tgcgtttgcc gacgtcgttc
   103201 ttaccgctgg aagaccgggg catgtttact acctcggtac aattgcccag cggttcaacc
   103261 caacaacaga ccctgaaagt cgttgagcaa atcgagaaat attacttcat ccatgaaaaa
   103321 gacaacatca tgtcggtgtt tgccaccgtt ggttctggcc ctgggggtaa cgggcaaaac
   103381 gtggcgagaa tgtttatccg cctgaaagac tggagcgaac gcgacagtaa gaccggcacc
   103441 tcgtttgcca ttatcgagcg tgcaacgaag gcgtttaacc aaattaaaga agctcgcgtt
   103501 atcgccagca gcccaccagc aattagcggt cttggtagtt ctgcagattt tgatatggag
   103561 ttgcaggacc acgctggagc gggtcacgat gcgctgatgg cagcacgtaa tcagttgctg
   103621 gcgctggcgg cggaaaaccc ggagctaacc cgtgtgcgcc ataacggcct cgatgacagt
   103681 ccgcagttgc agattgatat cgaccagcgt aaagctcagg cgctgggcgt tgatatcgac
   103741 gatattaacg acacactgca aaccgcctgg ggttcgagct atgtgaatga ctttatggat
   103801 cgcggtcgcg tgaagaaagt ctatgtgcag gcagctgcgc cgtatcgcat gctgccagat
   103861 gacatcaatc tctggtatgt ccgaaataaa gatggcggca tggtgccctt ctctgctttc
   103921 gcgacctcac gctgggaaac aggctcgccg cgtctggaac gctataacag ttattctgcg
   103981 gttgagattg ttggggaagc cgcaccgggg gtcagtaccg gtacggcgat ggatattatg
   104041 gaatcgttag tgaagcagct gccaaacggc tttggtctgg agtggacggc gatgtcgtat
   104101 caggagcggc tttccggcac gcaggctccg gcgctgtacg ccatttcctt gctggtggta
   104161 ttcctgtgtc tggcggcatt gtatgaaagc tggtcggtac cgttctcggt aatgctggtc
   104221 gtgccgctgg gggtaatcgg cgcgctgctg gcaacctgga tgcgcgggct ggaaaacgac
   104281 gtttacttcc aggtgggcct gttaacggtc attggtttat cggcgaaaaa cgccatcctg
   104341 atcgtcgagt ttgctaacga gatgaaccaa aaaggccacg acctgtttga agcgacgctc
   104401 cacgcctgcc gtcagcgttt acgcccgatt ctgatgacct cgctggcatt tatcttcggc
   104461 gtattgccaa tggcaaccag cacgggtgcc ggttccggtg gtcagcatgc ggtgggtact
   104521 ggcgtaatgg gcgggatgat ttcagctact attctggcta tttacttcgt gccgctgttc
   104581 tttgtgctgg tgcgccgccg cttcccgctg aagccgcgcc cggaataagc aataaaaaag
   104641 gcgacatgcc aatgtgtcgc ctttttcaac tttccgatta agaacctgct cagcgggttc
   104701 ttgctgttta tactttgtct caggaattac ttacgaagca taacttcgat aaagtctttc
   104761 cagttcccca gttcacgttc aatcataaca acctctctta taattatggg tattctacgg
   104821 aaaaaatata ccgtggtgaa gctaatttac tcgattgctg cgatgactac ctccggggga
   104881 caaaccttat gtaaatacta tggtcctgcg gtgataattt gtatgtgata cacagcaaca
   104941 tttcgagata ttcatacggc atctaatact tatttaattc tggttaaaat acagacagat
   105001 aacaagatga atattcttaa tgtttacgtt aaaaatgttt aatattattt aatagttgtt
   105061 aatttgaata cttcgataat gttatatttt ctgataatca tttgcaggca aaatgttttc
   105121 acccttaaat gagtatttat tctcataaat cgaaaaagga ttcattatgg ttacacttta
   105181 cggcatcaaa aattgtgaca ccattaaaaa ggctcgccgt tggctggaag ccaataacat
   105241 cgactatcgt tttcatgatt accgcgtcga tgggctggac agcgaattat tgaacggttt
   105301 tatcaacgaa ttaggctggg aagcgttact caacacccgt ggtacaacct ggcgtaaact
   105361 ggacgaaact acccgcaata aaatcaccga tgcggcctct gcggcggcat taatgactga
   105421 aatgcctgca attatcaaac gtccattgct ctacgcgcct ggtaagccta tgctgctggg
   105481 tttcagtgat tccagttatc agcaattttt ccatgaggtg tagtctatgt cgtgcccggt
   105541 tattgagctg acacaacagc ttattcgccg cccttccctg agtcctgatg acgcaggatg
   105601 tcaggctttg ttgattgaac gtttgcaggc gatcggtttt accgttgaac gcatggattt
   105661 tgccgatacg cagaattttt gggcatggcg tgggcaaggt gaaacgttag cctttgccgg
   105721 gcataccgac gtggtgccgc ctggcgacgc cgatcgttgg atcaatccgc cgtttgaacc
   105781 caccattcgt gacggcatgt tattcgggcg cggtgcggca gatatgaaag gctcgctggc
   105841 ggcgatggtg gtagcagcag aacgttttgt cgcacaacat cccaaccata cggggcgact
   105901 ggcatttctg atcacttctg atgaagaagc cagtgcccac aacggtacgg taaaagtcgt
   105961 cgaggcgtta atggcacgta atgagcgtct cgattactgc ctggtcggcg aaccgtcgag
   106021 tatcgaagtg gtaggtgatg tggtgaaaaa tggtcgtcgc ggatcgttaa cctgcaacct
   106081 caccattcat ggcgttcagg ggcatgttgc ctacccacat ctggctgaca atccggtaca
   106141 tcgcgcagca cctttcctta atgaattagt ggctattgag tgggatcagg gcaatgaatt
   106201 cttcccggcg accagtatgc agattgccaa tattcaggcg ggaacgggca gtaacaatgt
   106261 tattccgggt gaactgtttg tgcagtttaa cttccgcttc agcaccgaac tgactgatga
   106321 gatgatcaaa gcgcaggtac ttgccctgct tgaaaaacat caactgcgct atacggtgga
   106381 ttggtggctt tccgggcagc catttttgac cgcgcgcggt aaactggtgg atgcggtcgt
   106441 taacgcggtt gagcactata atgaaattaa accgcagcta ctgaccacag gcggaacgtc
   106501 cgacgggcgc tttattgctc gcatgggggc gcaggtggtg gaactcgggc cggtcaatgc
   106561 cactattcat aaaattaatg aatgtgtgaa cgctgccgac ctgcagctac ttgcccgtat
   106621 gtatcaacgt atcatagaac agctcgtcgc gtgatgagtg gttctgcaag aggaaataag
   106681 catggactgg ctggctaaat attggtggat tctggtgatt gtctttttgg tcggcgtcct
   106741 gctgaacgtg attaaagatc tcaagcgcgt cgaccataag aaatttctcg ccaacaagcc
   106801 ggagcttccc ccgcatcgtg atttcaacga taagtgggac gatgacgacg actggccgaa
   106861 aaaggatcaa ccgaagaagt aagctgagtg ctcaaccgta ggccggataa ggcgttaacg
   106921 ccgcatccgg caatggtgaa cgatgcctga tgcgacgctc acgcgtctta tcaggccagg
   106981 taatctgtct tttacatcat ttcaatcaca tcatcatcgc caggcttacc gccacttagc
   107041 gcttggtcga aataatgttt cggaatggta taacgcaagt gatccagggc aaattgcata
   107101 ctgcgattgt cgatcgcatg gcctaaatcc tccacgatat ccagcgtcac gtcaccaccg
   107161 gcactgatta acgcttcctg cgccgcaact gcatgcgcca gatcgataac cggatcttca
   107221 ccaccatgaa tcaagtgaat tgtcgtggct gtagacgcgg tctccggcag gctcgcatag
   107281 cgtccgttaa aggcaatgac gcgcgaagca aggcccggtt cggctttaat gctctccagc
   107341 gccataatcg cgccttgtga gaaaccaatt agcgccgtgg cgttagcccc tactccgctc
   107401 tgtttctgcc agtagcgtac cgtttcaata aacgtcggca tgatggcatc cacacgcgcc
   107461 tggcgattat cttccgtaat accctgcact gaaaaccact gacgccccgc cggattaccg
   107521 ctcggctccg cgccaccgac actcaccacc agcgcatcag gaaacagcgg tgcaaaccag
   107581 ctgcctattt cccccattgc caccgggtta tccccgacac cgtggaaaag cagtaacaac
   107641 tgttgtgcag gtttatccgg gctttgaaca acaaaatggt catgtttcat ggcgaactcc
   107701 ttaactgatg tcattaattt tacgccgctg actcacaatg accatgggag aaaattgaag
   107761 gagtcagtga aaaaattgcc attgcgttat gcgatcgcgc agacgctcag tgcgaacctc
   107821 atttagtgcg aacagcgcct gcgcggcctc ttcccgctgg cgaaccagta acatcttgcg
   107881 gcctgaaagt ttaagtgtgg tacataactg cgcgtcactg gcgtttttct gtaaacaccc
   107941 acgcagcgcc ggtaatgcca gttcactggt ttgtagcaga cgcaataagc aacctaacga
   108001 tgttaatagc ggacgatgag cgaaagcaaa accggccagt tcaagccagt cgtcgtcaga
   108061 aaggatggca tcgtttagtg gatcaacagg aagcatttcg ccattccact gcgcgagagc
   108121 ttgcgcatcg cgacgtaaac ggtaatgctc acgttcagcc agctgtttac ccgcatcact
   108181 catcggtaac agcgccatcg ccgtatagca accgctgctg gcttcccgat gattacccat
   108241 ccgcaccagc acaaaaccgc agcgttgcca gaaacgccat aactccccag tgtaaccaaa
   108301 actcaccgaa agatagtcga ggtcatgcgt atattgcaaa gctccagcaa taagttgccg
   108361 ccctgtgcct tcccgctgac gagccggatg aactgctatc cggctaaccc gccttccgcg
   108421 caatgtcgcc gccagtggat tgctgccgtg cgccgccagc gactgggcca ccagattacc
   108481 ccgcgggcga cgaaaacctg cccataccgc ctgactgagt tcttgagata atccgccctc
   108541 atccaccagc cacaacgccc cggcaatctc gttttcgcca gccgcctgta aaaaatgttg
   108601 ccctagtgca tccatcatcc ggcgtaaatc cagcggcgag gtccggtagt gcgcaccaga
   108661 caataactga taaaccttta acggcgtttc tggctcgctt cgccataacg tttgttcaaa
   108721 tgcggaaatg acgatattgc cttgtggtgt atgggtgaag ttttcatcgt taaaaaccag
   108781 tgcctcacta accatttttt ccagcgggca tccctgtgcc cagcggatcg gctgttgcag
   108841 ttcaaaacgg tgtaaatgcg gaaagcgagc gcaaaatttc agcaaaaaac cacgtccggt
   108901 accttcgtag ccctgcaccg tagtggttaa caacgttcga ggaaaacgcg ataccagttg
   108961 atgcaacaac ggcgcaggta tggctgcggc ttcgtcgacc accagccagt cggcttgctc
   109021 atcgctggct aacaaggcat ccggtgcaat aaagcgaaac ttctcgcccg caaattgtgc
   109081 cagtacatcc gttgccgctt ttgcgggcgc ggtgacaatc gcactgcccg caatacgaga
   109141 aatgagttgc cctgccaacg ccgacttacc gcgcccacgc gcagccgtta ccgctgccac
   109201 gcccggcggc atggtcagta gctgctgtaa gagttgctgt tgttctggtt gtggtgcgcc
   109261 ggtcgcgggg tgccagtcag tacggggagt aaaatgcgcc aacgagaacg gctggttttg
   109321 ccgccagagg atagcgtcgt tatccgccgt aagtacgcgt ttgagatgct ggacaaaatg
   109381 cggcgtcgca atagggtcag ggcaatcact ccagcgcagc gagtcggcat caggttggtt
   109441 ttcccactct tcccatacag ggagtaacaa aaccagccag cttcccgctt tcaacgttcc
   109501 gctaagtgct gcaaaggcag cggcatcaaa gccgtggcgg gcgtcgaata ccgcatgccg
   109561 gaactcgcgc ccaagtaaag tttgtagcgc cgagggtgaa cagtggtttt cagcatctag
   109621 ctgcggcgaa atccacagcc agtcgccagg taaggcatca cgcaacttaa gagcatgatc
   109681 aaaacaccaa ccctcttctc cgctcaacac cagcaagcgg cagatccctt cacgtttcat
   109741 ttgcgctgtt aatgtgtaaa gcgcagtcag ttcagccatc cctgccccgg aaattaaatg
   109801 cttttaccaa aagtattgca ttgtgccgga tcgccgctgt cgaaaccacg tttaaaccag
   109861 ctgcagcgtt gctgagaagt gccatgtgtg aaactgtctg gtactactcg cccctgactt
   109921 tgctgttgta aacggtcatc gccgatggcc tgcgccgcat tcagcgcctc ttccagatcg
   109981 ccagtttcca gaacgccttg ctgctgcata ctatgccccc agacaccggc aaaacagtcg
   110041 gcctggagtt ccatacgcac agataagcgg tttacttccg cctgcgtcgc gttttgttgc
   110101 agttgacgaa ctttcggctc gatgcctaac agtttctgca catgatgacc gacttcatgg
   110161 gcgataacgt acccctgggc aaaatcgcca tccgcgccaa gtttgtcttt catgtcatca
   110221 tagaaggaga gatcgatata aactgtgcca tccgccgggc aatagaacgg ccccataatg
   110281 gactggcccg ccccgcagcc ggtgcgcgtc attccacggt acatgaccag cttcggttgc
   110341 tgataggtct tacccatctt ttcgaactgt tgtccccagg tgtcttccgt ggttgccaga
   110401 atcaccgagg tgaattttgc ggcttcgtct tcattagggc taattgaccg cgttgattgt
   110461 tgttgagaaa ccggctgccc ggtcatcaac ccggttaaat caacgccata gtagcctgca
   110521 accagcacga ctatcagtaa aatcagcccg cctttaccgc tcggcagacg gaaaccggga
   110581 ccgcccattg acggaccgcc agagctgttg cgcctgtctt caacattgtc actttcacgt
   110641 cgcccttgcc aacgcataga tacctcaaca atatattcat tatagaaatg atcgtaggtg
   110701 gttcagagga agattaccac aggaaagcgg gatgaaatgg cggaggaatg agcgttcaat
   110761 gcatatattc cgagagcggt tgtcttgccg tgccagctgc acggcaagat gatgaacaga
   110821 aaaatcagtc cagctgtaca cccaggcggc gggcgacggc ttcataggct tcgatcaggc
   110881 caccgaggct ctggcggaaa cggtctttgt ccattttctc cagtgtttct ttgtcccaca
   110941 ggcggctgcc gtccggggag aactcatcgc ccagtaccac ttcacctttg tacagaccaa
   111001 actccagctt gaagtcgacc agaatcagac cagcatcatc gaacagtttt ttcagcacgt
   111061 cgttcgcttt gtaggtcagc tctttcatac gtgccaggtt ctctttgctc acccagccaa
   111121 aggtttcgca gtaagattcg ttgaccatcg gatcgtgcat ggcatcgttt ttcaggaaca
   111181 gatcgaacag cggcgggttc agctcaatac cttcttcgat tccaagacgt ttcaccagag
   111241 agccagcagc acggttacgc acgacacact caaccggcac catatccagc tttttcacca
   111301 gacattcggt atcggagagc agacgctcca tttgagtcgg gatacccgct tcagccagtt
   111361 tgctcataat gaagtagttg aacttgttgt tcaccatacc tttgcgatca aactgctcaa
   111421 tgcgcgcgcc atcccctgct gacgtatcat tgcggaattc gagcaccaac aggtccgggt
   111481 tttccgtgct gtatacggtt ttcgctttac cacgatacaa ctcagcttgc ttttgcatct
   111541 ttatcactcc tgggtgtgaa ttaacgtttt aaaatctttt gctgtctggt gtgccggatg
   111601 ttttttcgga tgcggcgtga acgccttatc cgaactacgt ccgacaaaca aatttcgtgc
   111661 gaattaccgc taaaatcgcg ttttcctgcc gacgcacacg tttgcgtatc atatcagaaa
   111721 aaagggccgg atgattccag ccctgtattt ttacttgcta aacgcagcct ggaagacagc
   111781 taccagcgcg tcgttctgac tctgagtcag agtatgacct ttcggatcga tgaactgtag
   111841 gctgctgcgg ttatctaaat cgccaacctg cagtttatag tcaccggatg ccaggcctgg
   111901 atcgctcgcg cccagttcct gccagtcgct gtcagacagc ggcttataag ttacggccat
   111961 gttgccctgc gaacgggtgc tgtcggtcac tttcatgccc actttttcca gcgacgctgg
   112021 cagacgttgc caaaccacat tgaacggccc gcgtacgacc agcattggta aaccggtgtc
   112081 atcagctgca ctttgtacgt ccatagtggt ggaggcacga ttttgcgcag cgttcgcggc
   112141 gtcagtggca gatttatcca gaccggcgga aataacgttc atcatctccg tgctgtaacg
   112201 ctgcatggaa gccgcgtctg caaccggttt gcccgcctgt tccaggttca gcagtttaac
   112261 cgtaaccgcc tgctgataac cctgcggctt aacagagatt tgataacgac cacgatactg
   112321 ctcgtcttcg tccagacggt tccattgtac ccaatcggtg gtcagtgtct gaccagcatc
   112381 atcacgttgg gtgatggtgt agtttttcgc ctgcagcacg ctaaccacct gcggccacag
   112441 agtattgcca cgaccatttt ccaccagcaa tgaagcggta tcgcccgtga actgggtacg
   112501 cgcgccagaa accagtgcca gcggctgggc tggtggacga atgtccagcg ccttaccgac
   112561 agcaccacta ccgttggtca ccgggattgc ataatcaccg gaggtcaccg gcaaaatcat
   112621 tccagccggg gcatgaagct ccgcaagcgg tgccgcttcc aggtaggctt catcaccact
   112681 gacctgacgc ttatagcgtg agtcagaact acaggcagcg agtaataaaa caagcgaaac
   112741 acccgcaacc tttgccaggc gcgacttttg aacagagtaa gccatcaaat ctccctaaac
   112801 tttacagcaa accggcatgc ttaagcgccg ctctgaccgt ctcacgacca ctgtcggtga
   112861 ttggtgtcat tggcaggcgc agcgtatcgg tcgccacaag acccagttcc ttacatgccc
   112921 atttcaccgg gattggattg ggttcgacaa atagtttgtt gtgtaatggc atcagacgct
   112981 gattaataac gcgtgcctcg gcaaaatgcc cttctgctgc cagtttgcac atctgggcca
   113041 tatcacgcgc tgcgacgtta gccgtaacgg aaataacccc atgaccgccc aattgcatga
   113101 agtccagcgc gctcgcatca tcgccgctca gcagaacaaa atcatctgaa accagctctt
   113161 tgatctggtt tacacgcgtt aagttccctg ttgcctcttt gattccgata atatttttta
   113221 ctttcgccag acggcccacc gtttccggga gcagatcgca gccagtacgg gacggcacat
   113281 tatacagaat ttgcggcagg tcagtatgct cagcgatggc tttgaaatgc tgatacaaac
   113341 cttcttgcga cggacgattg tagtaagggg ttaccgtcag gcagccgacg ataccactgt
   113401 cattgaagcg ctgcgtcagg ctaatggctt ccgcagtagc gttagcgccg gtcccggcaa
   113461 ttaccggaat gcgcccatca gccagatcca gcgtcatcat caccacatca gcatgttcgt
   113521 catgatttaa ggtagcggac tcgccagtgg tgccaacaga aacgatcgcc gaagtaccgc
   113581 tggcgacatg ataatcaatc agttttttca agctagcccg acagacatta cctttttcat
   113641 ccatcggagt aacaatcgcg acaatacttc ccgtgaacat gggccatcct ctgtgcaaac
   113701 aagtgcctca atggtacgtt tggtatggca ttaaaagcaa gcagacagaa ccgttctgat
   113761 tgttgtatgc atgatttttt tatgctttcc ttaagaacaa ctcacccctt aaaggaataa
   113821 ccagtttgac actgtcatcg caacattatc tggtgatcac tgcgttgggt gccgatcgcc
   113881 ctggaattgt gaacaccatc acccgtcatg tcagtagttg cggctgtaat attgaagaca
   113941 gtcgcctggc gatgctggga gaagagttca cgtttatcat gctgctttcc ggttcatgga
   114001 atgccatcac tctgattgaa tcaacgttac cgttgaaagg tgccgaactg gatcttttaa
   114061 tcgtgatgaa gcgcacgacg gcgcgtccgc gtccgccaat gccagcatct gtctgggttc
   114121 aggtcgatgt tgcagactcc ccgcatttaa ttgaacgctt cacagcactt ttcgacgcgc
   114181 atcatatgaa cattgcggag ctggtgtcgc gcacacaacc tgctgaaaat gaacgggctg
   114241 cgcagttgca tattcagata accgcccaca gccccgcatc tgcggacgca gcaaatattg
   114301 agcaagcgtt caaagcccta tgtacagaac tcaatgcaca aggcagtatt aacgtcgtca
   114361 attattccca acatgatgaa caggatggag ttaagtaatg aatccactga aagccggtga
   114421 tatcgcaccg aaatttagct tgccggatca agacggagaa caagttaatt tgaccgactt
   114481 ccagggacag cgtgttctgg tttatttcta cccgaaagcc atgacccccg gctgtaccgt
   114541 acaggcctgc ggcttacgcg ataacatgga tgagttgaaa aaagcgggcg ttgatgtgct
   114601 gggtatcagc accgataaac ccgaaaaact ctcccgtttt gcggaaaaag agctgcttaa
   114661 ctttacgctc ctgtctgatg aggaccacca ggtgtgcgaa caattcggcg tctggggtga
   114721 aaagtccttc atgggcaaaa cctacgatgg tattcatcgc atcagcttcc tgattgacgc
   114781 tgatggcaaa atcgaacatg tctttgacga tttcaaaacc agcaatcacc acgacgttgt
   114841 gctgaactgg ctgaaagaac acgcctgatt actttgctcc attccgtgct ggctgcgctt
   114901 gcggccagca tacctcactt ctcgtgatca agatcacatt ctcgctttcc cctgcgacac
   114961 gggtgtcgaa tccatttttt gctgaacgtt aatgaccatc atttttgtac cgttcagaat
   115021 ccagttaata cataacttat tgaatagatt gagttaatca gaatggcacc ctttatgcaa
   115081 tatgaaatgc aatgtttcat atcattttca aggagccgac atgaaccgct ttgtggtggc
   115141 cgaaccactg tggtgtacag gatgtaatac ctgtctcgct gcctgttcgg acgtgcataa
   115201 aacgcaaggt ttacagcaac acccgcgcct ggccctggcg aagacgtcaa caatcactgc
   115261 ccctgtcgtg tgtcatcact gtgaggaagc cccttgcctg caggtctgcc cggtcaatgc
   115321 catctctcag agggatgatg cgatccaact caacgaaagc ctctgtattg gctgcaagct
   115381 ttgcgccgtg gtctgcccat ttggcgcaat cagcgcttca ggaagccgtc cggtgaatgc
   115441 ccatgcgcaa tatgtttttc aggctgaagg ctcactcaaa gacggcgaag aaaacgtgcc
   115501 gccccaacat gcgttgctgc gctgggaacc gggtgtccag accgtcgcgg tgaaatgcga
   115561 cctgtgtgat ttcttgccag aaggtccggc ctgcgttcgc gcttgcccga atcaggcgtt
   115621 acggctgatc accgacgata gcctgcaacg tcagatgaaa gaaaaacagc gccttgccgc
   115681 aagctggttt gccaatggcg gggaggatcc cctttccctc actcaggagc aacactaatg
   115741 gatgccctgc aattattaac ctggtcgctg atcctctatc tgtttgccag tctggcttcg
   115801 ctgtttttac tcggtctgga cagactggct attaagcttt ccggcatcac atcgctggtg
   115861 ggcggcgtga ttggcatcat cagcggaatt acgcaattac atgctggtgt aactttagtc
   115921 gcccattttg ccaccccttt tgactttgcc gatttaaccc tgcgaatgga tagcctctcg
   115981 gcatttatgg tgctggttat ctccttgctg gtggtggttt gttcgctcta ttcattgact
   116041 tatatgcgcg aatacgaggg caaaggcgcg gcggcgatgg gcttctttat gaatcttttc
   116101 atcgcatcga tggttgccct gctggtgatg gacaacgctt tttggttcat cgtgctgttt
   116161 gaaatgatgt cgctgtcttc ctggtttctg gtcattgcca ggcaggataa aacgtcgatc
   116221 aacgctggca tgctctactt ttttatcgcc cacgccggat cggtgctgat tatgatcgcc
   116281 ttcttgctga tggggcgcga aagcggcagc ctcgattttg ccagtttcgc acgctttcac
   116341 tttctccggg gctggcgtcg gcggtgtttc tgctggcctt tttcggtttt ggcgcgaaag
   116401 ccgggatgat gccgttgcac agctggttgc cgcgcgctca ccctgccgca ccatcgcacg
   116461 cttcggcgtt gatgtctggc gtaatggtca aaatcggtat tttcggcatc ctgaaagtag
   116521 cgatggatct gctggcgcaa acgggtttgc ctctgtggtg gggcattctg gtgatggcga
   116581 tcggcgcaat ctccgcgctc ctgggcgtgc tatatgcgct ggcggaacag gatatcaaac
   116641 ggctgctggc ctggagtacc gtcgaaaacg tcggcattat tttgctggca gtcggtgtgg
   116701 cgatggacgg tctgtcactg cacgacccgc tgctcaccgt ggttggactg ctcggcgcac
   116761 tgtttcatct gctcaaccat gcgctgttca aagggctgct atttctcggc gcggacgcga
   116821 ttatttcgcg tttgcatacc cacgacatgg aaaaaatggg ggcactggcg aaacggatgc
   116881 cgtggacagc cgcagcatgc ctgattggtt gcctcgcgat atcagccatt cctccgctga
   116941 atggttttat cagcgaatgg tacacctggc agtcgctgtt ctcactaagt cgtgtggaag
   117001 ccgtagcgct acaacttgcg ggtcctattg ctatggtaat gctggcagtc actggtgggc
   117061 tggcagtaat gtgcttcgtc aaaatgtacg gtattacttt ctgtggtgcg ccgcgcagta
   117121 cacacgctga agaggcacag gaagtgccaa atacgatgat cgtcgccatg ctactgctcg
   117181 cggcactctg cgtattaatt gcgcttagtg ccagttggct ggcaccgaag ataatgcata
   117241 ttgcccatgc gtttaccaat acccctcccg tcactgtcgc cagcggaata gcacttgtac
   117301 ccggcacgtt tcatacacgg gtcactcctt cattactgtt gctgttacta ctggcgatgc
   117361 ctttgctgcc aggcctttac tggctgtggt gtcgttcgcg ccgcgcagcg tttcgtcgca
   117421 caggagatgc ctgggcatgc ggctacggct gggaaaacgc gatggctccg tcaggcaatg
   117481 gcgtgatgca gccgctgcgt gtggtcttct ctgcgttatt tcgtctacga caacagctcg
   117541 accctacgct gaggctaaat aaaggtcttg cgcacgtcac cgccagggct cagagcacag
   117601 aacccttctg ggatgagcgg gtgatccgcc ccatcgtgag cgccacccaa cggctggtca
   117661 aagaaataca gcatctgcaa agcggcgact ttcgtctcta ttgcctgtat gtggtcgccg
   117721 cactggttgt gctgctaatc gctattgccg tctaaggaaa tcaccatgag acaaactctt
   117781 tgcgacggat atctggtcat ttttgcgtta gcacaggccg tgattctgct gatgctaacc
   117841 ccacttttta cgggtatttc ccggcagata cgcgcgcgta tgcactcccg ccgcgggccg
   117901 gggatctggc aggattatcg cgatatccac aaactgttta aacgccagga agttgcgccg
   117961 acatcttcag gtctgatgtt ccgcctgatg ccgtgggtat taatcagcag catgctggtg
   118021 ctggcgatgg ccttaccact atttattacc gtttcccctt ttgcgggcgg cggcgatctg
   118081 atcaccctta tctatcttct tgccctgttt cgttttttct ttgctctttc cgggctggat
   118141 accggaagtc cgtttgcggg agtcggtgcc agtcgcgagt tgacgctcgg cattctggtc
   118201 gaaccaatgc ttattctctc actgctggta ttggcgctga tagcaggttc cacgcatatc
   118261 gagatgatca gcaatacgct ggcgatgggc tggaactcgc cgctaaccac cgtactggcg
   118321 ttactggcct gtggttttgc ctgcttcatt gagatgggaa aaattccctt tgatgttgct
   118381 gaagcagaac aggaattaca ggaaggcccg ctgaccgaat attccggtgc cgggctggcg
   118441 ctagcgaaat gggggcaggg gctgaaacag gtcgtgatgg cagcactgtt tgtggccctg
   118501 tttctgccct ttgggcgcgc gcaagaactt tctctcgcct gcctgctgac ttcacttgtc
   118561 gttacgctgc tcaaggtttt gctgattttt gtactggcct caatcgcaga aaacacgctg
   118621 gcacgcgggc gttttttact cattcaccat gtgacctggc ttggcttggc ttcagccttg
   118681 ctgcgcttgc atgggtcttc tggttaaccg gtctgtaagg agcactgacg gaatatggaa
   118741 aatcttgctc tgacgacgtt aatgctgcct tttatcggcg cactggtcgt ttcgttttcg
   118801 ccacaacgtc gggccgccga atggggggtt ttgttcgccg cgctgaccac gctgtgcatg
   118861 ttgtcgctga tctccgcgtt ttatcaggcc gataaagttg ccgtcacgtt gacgttggtc
   118921 aacgtggggg atgtggcatt gtttggcctg gtcattgatc gcgtgagtac gctgattctg
   118981 tttgtggtgg tgtttctcgg tttgctggtc acgatctact ccacgggtta tctgacggat
   119041 aaaaatcgcg aacacccgca taacggcacg aatcgttatt acgcattttt gctggtgttt
   119101 atcggcgcga tggcgggact ggtactctcc tcgacgctgc tcggtcagtt gttgtttttt
   119161 gaaattacgg gcggctgctc ctgggcgttg atcagttatt accagagcga taaagcgcag
   119221 cgttcagcac taaaagcgtt gcttatcacc catatcggtt cgttggggtt gtatcttgcc
   119281 gccgccacgc tgtttttgca gaccggaacg tttgtgctta gcgcgatgag cgagttacat
   119341 ggcgacgcac gttatctggt ttatggcggc atcctgtttg ccgcgtgggg gaaatcggcc
   119401 cagctaccga tgcaagcgtg gctaccggac gcaatggaag cgccaacacc gatcagcgac
   119461 tatctccacg ccgcatcgat ggtgaaagtg ggcgtttaca tttttgcccg cgccattatc
   119521 gacggcggca atatcccgca tgtgattggc ggcgttggca tggtcatggc actggtcacc
   119581 attctttatg gctttctgat gtatttgcca cagcaggata tgaagcggtt gctggcctgg
   119641 tcgaccatca ctcaacttgg ctggatgttc ttcggcttgt cgctctccat cttcggctcg
   119701 cggctggcgc tggagggtag catcgcctac atcgtcaacc acgcgttcgc taaaagcctg
   119761 tttttccttg tagcaggtgc gctgagttac agctgcggca cgcgcttgtt gccgcgtctg
   119821 cgtggcgtat tgcacaccct gccgttgcca ggcgtgggtt tctgcgtggc agcgctggca
   119881 attaccggtg tgccgccgtt caacggcttc ttcagtaaat tcccgctgtt tgccgccggt
   119941 tttgcgttgt cagtggagta ctggatcctg ctgcccgcca tgattctgct gatgattgaa
   120001 tcggtcgcca gtttcgcctg gtttattcgc tggtttggtc gcgtcgtgcc gggcaaaccg
   120061 agcgaggccg tcgccgatgc cgcaccgctg ccagggtcaa tgcgcctggt gttgattgta
   120121 ctgattgtga tgtcgctgat ttccagcgta atcgccgcga cctggttgca gtaaggagat
   120181 gatgaatgac cggttctatg atcgtaaata atctggcggg actgatgatg ctgacatcgc
   120241 tgtttgtgat tagcgtcaaa agctatcgcc tgtcatgcgg attttacgcc tgccagtcgc
   120301 tggtgctggt gtctattttc gccactctct cgtgcctgtt cgccgcagag caactgctga
   120361 tctggtccgc cagcgccttt atcaccaaag tgctgctggt accgttaatc atgacttacg
   120421 ctgcacgaaa tattccccag aacatcccgg aaaaagcgtt attcggtccg gcaatgatgg
   120481 cactgctcgc ggcgttaatt gtcctgcttt gcgcatttgt cgttcagccc gtgaagctac
   120541 cgatggctac cgggctgaaa ccggcgctgg cggtagcgtt aggtcatttt ctgcttggcc
   120601 tgctgtgcat tgtcagccag cgcaatatcc tgcggcaaat ttttggttac tgcctgatgg
   120661 aaaacggctc ccatctggtg ctggcgcttc ttgcctggcg agcaccggaa ctggtggaaa
   120721 taggtatcgc taccgacgcc atcttcgccg tcattgtgat ggtgttactg gcaagaaaaa
   120781 tatggcgtac ccacggcacg ctggacgtga acaacttgac cgcgctgaag ggataatgag
   120841 atgagttatt ctgtgatgtt cgctttactc ctgctcacgc cgctgctttt ttcgctgctc
   120901 tgttttgcct gccggaaacg ggggctctct gcgacttgta cggtgactgt attacatagc
   120961 ttagggatca cactgctgct gattctggca ctctgggtgg tccaaactgc cgctgatgca
   121021 ggagaaatat tcgctgcggg actgtggctt catattgatg gtctgggcgg tttgttcctc
   121081 gccattcttg gtgtgattgg ctttctcacc ggtgtttact cgattggcta catgcgtcat
   121141 gaagtggcac acggcgagct ttcacccgtt acactgtgcg attactacgg tttcttccat
   121201 ctgttttttt tcaccatgct gctggttgtt accagcaata acctgattgt gatgtgggcg
   121261 gcgatcgaag ccaccacctt aagctcggcg tttctggtag gcatttacgg tcagcgttca
   121321 tcgctggaat cagcatggaa gtacatcatt atttgtactg ttggtgtcgc ttttggtctg
   121381 ttcggtaccg tgctggtata cgccaacgcc gccagcgtta tgccgcaggc agaaatggcg
   121441 atattctgga gcgaggttct taagcaatcg tccttgcttg acccaacatt aatgctgttg
   121501 gcctttgtgt ttgtgctaat tggctttggt accaaaaccg ggctatttcc catgcacgcc
   121561 tggctgccgg atgctcacag tgaagcgccg agtccggtca gcgccctgct ctccgccgta
   121621 ttgctgaact gcgcgctgtt ggtgctgatt cgctattaca tcattatttg ccaggccatc
   121681 ggcagcgatt tccccaaccg gttgttgctc atcttcggca tgttgtcggt tgccgtggcg
   121741 gcatttttca ttctggtaca gcgggacatt aagcgtctgc tggcgtactc cagcgtggag
   121801 aacatggggc tggtcgcggt ggcgctaggc attggcgggc cgctgggaat ttttgccgcg
   121861 ctgctgcaca ccttaaacca cagtctggca aaaacgctgc tgttctgcgg ttccggcaat
   121921 gtactgctca agtacggcac gcgcgatctc aacgtcgtct gtgggatgct caaaatcatg
   121981 ccatttaccg ccgtgctgtt tggcggcggt gcgctggcgc tggcagggat gccgcccttc
   122041 aacatttttc ttagcgaatt tatgaccgtt accgcaggac tggcacgtaa tcacctgctg
   122101 attatcgtcc tgctgttatt gctgttaacg ctggtgctcg cgggcctggt acggatggct
   122161 gcgcgggtgt taatggcgaa accgccgcag gccgttaacc ggggtgatct cggctggttg
   122221 accacctcgc caatggtgat tctgctagtc atgatgctgg cgatgggaac gcatattcca
   122281 caacctgtca tcaggatcct ggcgggcgct tccactatag tcctctcagg gacgcacgac
   122341 ctgcctgcac aacgtagcac ctggcatgat tttttgcctt caggcaccgc atctgtttcg
   122401 gagaaacaca gtgaacgtta attcatcgtc aaatcgtggc gaagcgattc tcgccgccct
   122461 gaaaacgcag ttccccggcg cggtgctgga tgaagagcga caaacgcctg aacaggtcac
   122521 cattacggtg aaaatcaatc tgctgcctga cgttgtacat tatctttatt atcaacatga
   122581 tggctggctt ccagtcctgt ttggcaacga cgagcggaca cttaacggtc attacgcggt
   122641 ttattatgcc ctttcaatgg aaggggccga aaaatgctgg atcgtggtga aggcactggt
   122701 cgatgccgac agtcgggagt ttccgtcagt cacaccgcgc gtccctgccg cggtctgggg
   122761 cgagcgagaa attcgcgata tgtacgggct gattccggtt ggcctgccgg atcagcgtcg
   122821 cctggtgttg cccgatgact ggccggaaga tatgcatccg ctgcgcaaag atgcgatgga
   122881 ttatcgactg cgccctgaac cgacgacaga ttccgaaacg tatgcgttta tcaatgaggg
   122941 caacagcgat gcgcgggtga tccctgtcgg cccgctgcat atcacctcct atgaaccggg
   123001 tcacttccgc ttgtttgtgg atggcgagca aattgtcgat gctgattacc gcctgtttta
   123061 tgtccatcgc ggcatggaga aactggcaga aacgcggatg ggctacaacg aagtgacctt
   123121 cttatcggac cgcgtgtgtg ggatttgcgg ttttgcccac agtgtggcct ataccaactc
   123181 ggttgaaaat gcactgggga ttgaggtgcc gcaacgagca catactattc gctcgattct
   123241 gctggaagtc gagcggctac acagtcattt gctcaacctt ggcctctcct gccattttgt
   123301 tggttttgat accggcttta tgcaattttt ccgcgtgcgg gaaaagtcga tgacgatggc
   123361 ggaattgctg accgggtcgc gtaaaaccta cggtctgaat ctgattggtg gtgttcgccg
   123421 cgatattctc aaagagcaac gtctgcaaac gctgaaactg gtgcgcgaga tgcgctccga
   123481 cgtgtcggag ctggtagaga tgctgcttgc tacgccgaat atggaacaac gcactcaggg
   123541 cattggcatt ctcgaccgac aaatcgcccg tgattatagc cctgtagggc cgctgatccg
   123601 cgcagtggtt ttgcccgtga tttgcgcttt gatcacccct acgccgacta cggcaatatt
   123661 ccaaaaacac tgtttacctt taccggcggc gatgttttct cccgcgtgat ggtccgtgtc
   123721 aaagagacgt ttgattcgct ggcaatgctg gaatttgccc tcgacaacat gccggatacc
   123781 ccactgctga ccgaaggctt tagctataaa cctcacgcat tcgcgctggg ctttgttgaa
   123841 gcgccacgcg gtgaagacgt gcactggagc atgctcggtg ataaccaaaa attgttccgc
   123901 tggcgctgtc gtgccgccac ctacgccaac tggccggtgt tgcgttacat gctgcgcggc
   123961 aataccgttt ctgacgcacc gctgattatc ggtagccttg atccctgcta ctcctgtacc
   124021 gaccgtgtga cgctggtaga tgtgcgcaag cgccagtcaa aaaccgtgct gtataaagag
   124081 atcgaacgct acggcattga tcgtaaccgt tcgccgctga agtaaggaca gaagatgctg
   124141 aagttactga aaactattat gcgcgccgga accccgacgg tgaaatatcc cttcgcgcca
   124201 ctggaggtca gcccaggctt tcgcgggaaa ccggacctga tgcccagcca atgtattgcc
   124261 tgcggtgcct gcgcctgtgc ttgtccggca aatgcgctga ctatccagac cgacgaccag
   124321 caaaattcgc gcacctggca gctctatctg gggcgttgta tttactgcgg acgttgtgaa
   124381 gaagtgtgcc cgaccagagc catccagctt accaataact ttgaactgac cgtcacccat
   124441 aaagccgatc tctataccca cgcgacgttc catctacaac gttgcagccg ttgcgaacgc
   124501 ccgtttgccc cgcaaaaaac cgtcgcactg gctgctgaat tgttggcaca gcaacaaaat
   124561 gctccacaaa accgcgaaat gttatgggcg caagcgagcg tctgcccgga gtgcaaacaa
   124621 cgcgcgacgc tgatcaacga cgatacagat gtaccgctgg tggctaagga gcagctatga
   124681 gtccagtgct tacgcaatat gtcagccagc ccatcacgct ggacgagcaa acgcaaaaga
   124741 tgaagcggca tttgctacag gatatccgtc gctcggctta cgtttatcgc atcgattgcg
   124801 gcggctgcaa cgcctgtgaa atcgaaattt ttgctgccat cacccccgta ttcgacgcag
   124861 aacgttttgg cattaaggtt gtttcatcac cgcgtcacgc cgatattttg ttatttactg
   124921 gcgcagtcac ccgggcgatg cgtatgcctg cacttcgggc gtatgagtct gcccccgatc
   124981 ataaaatctg tgtttcctac ggcgcgtgcg gtgtcggcgg cggtattttc cacgatctct
   125041 acagcgtctg gggcggcagc gacaccattg tccccattga tgtttggatc cccggctgcc
   125101 cgccaacacc ggccgccacc attcacggtt tcgccgtggc gctcggtttg ctgcaacaga
   125161 agattcacgc ggtggattat cgcgatccca ccggggtgac tatgcaaccg ttgtggccgc
   125221 agatcccgcc atcacagcgt atcgccattg agcgagaagc gcggcggctg gcgggctatc
   125281 gtcaggggcg agaaatttgc gatcggctcc tgcgccattt aagcgacgat cctacaggaa
   125341 atcgggttaa cacctggttg cgcgatgccg acgatccacg tctcaatagt atcgttcagc
   125401 aactctttcg cgtactccgg gggttacatg actgaagagt gcggggaaat tgttttctgg
   125461 acgctgcgaa aaaagtttgt cgccagtagc gacgagatgc cggaacacag ctctcaggta
   125521 atgtattact cgctggctat cggtcatcac gttggcgtga ttgattgtct gaatgtcgcc
   125581 ttccgctgcc cactgacgga atacgaagat tggcttgcac tggtcgaaga ggagcaagcc
   125641 cgacgtaaga tgctgggggt gatgactttt ggtgagattg ttattgacgc cagccacacc
   125701 gccctgttga cccgggcatt cgcgccactg gcagatgacg cgacgtctgt gtggcaagcg
   125761 cgtagcattc aattcattca tctgttggat gaaattgtgc tggaaccggc tatctatctg
   125821 atggccagaa aaattgcgtg agaaggattt ctcattaata aggactgttg atggctatgt
   125881 cagacgaagc gatgtttgcc ccgccgcaag gaataacaat tgaagcggta aacggaatgc
   125941 tcgcggagcg gttagcgcag aaacacggca aggcgtcttt attacgcgcc ttcatcccgc
   126001 tgccgccgcc gttcagcccg gtacaactta ttgaactgca tgttctcaaa agcaacttct
   126061 attaccgcta ccatgatgat ggcagcgatg tgacggcaac aacagagtat cagggcgaga
   126121 tggtcgatta ttcgcgtcac gccgtccttc tcggcagtag ttgaatggcg gagctacgct
   126181 ttattcgtac ccacggcagc cgttttactc cccaggattg cacactgttt aactggctgg
   126241 cacgcattat caccccggtt ctgcaatcat ggctcaatga tgaagaacag caggtggcgc
   126301 tgcatttgct ggagaaagat cgcgatcatc atcgggtact ggtggatatc actaatgcag
   126361 tgctgtcaca tcttgatctc gacgatctga tcgctgacgt cgctcgtgag atccatcatt
   126421 ttttcggtct ggcttcagtc agtatggtac tgggcgatca tcgaaagaac gagaagttca
   126481 gcctgtggtg cagcgatctt tctgcctcac attgtgcgtg tctgccacac aatatgcctg
   126541 gcgacagtgt attgctgaca caaacgctac aaacccgaca accgaccttg acgcaccgtg
   126601 cagatgatct gtttctctgg caacgcgacc cgttattact cttacttgca tctaacggct
   126661 gcgaatctgc gctccttata ccgcttacct ttggcaacca tacaccgggt gcattgttgc
   126721 tggcgcatac ctcttccact ctctttagtg aggaaaactg ccagctacta caacacatag
   126781 ccgatcgcat cgctattgcc gttggcaatg ccgatgcctg ccgtagaatg accgatttgc
   126841 aggaaagttt gcagcaagaa aaccaccagc ttagcgagca gctcctttcg aatctgggcg
   126901 tcggtgacat tatctatcaa agccaggcaa tggaagacct gctccagcag gtagatattg
   126961 tggcgaagag cgacagtacg gtgttgattt gcggtgaaac cggaaccggc aaagaggtga
   127021 tcgccagagc gatccatcaa cttagcccgc gacacgacaa gccgctggtc aaaatcaact
   127081 gcgctgccat ccccgccagt cttctggaaa gtgagttatt cggtcatgac aaaggggcgt
   127141 ttactggtgc gattaatacc catcgtggtc gttttgaaat tgccgatggc ggcacgttgt
   127201 ttctcgatga aattggcgat ctgccgttag aacttcagcc taaactgctg cgcgtattgc
   127261 aggaacggga gattgagcgt ctcggcggga gtagaacgat cccggtaaat gtcagagtca
   127321 ttgccgccac caaccgtgat ttgtggcaaa tggttgaaga tcgccagttt cgcagcgatc
   127381 tcttttatcg cctgaatgtc ttcccactgg aattgcctcc gctgcgcgac cgtccggaag
   127441 atatccctct tttagcaaag catttcacgc aaaaaatggc gcgccatatg aatcgttcaa
   127501 ttgacgccat cccgaccgag gcgctacgcc agttgatgtc gtgggactgg ccgggcaacg
   127561 tgcgcgagct ggaaaacgtg attgagcgag cggtactgct gactcgcggt aacagtctga
   127621 atttacatct taatgtccga caaagccgtt tactgccgac gctaaatgaa gattcagcgc
   127681 ttcgcagttc aatggcgcag ttgctgcacc cgacgacgcc agagaatgac gaagaagaac
   127741 gtcagcgcat tgttcaggta ttgcgagaaa ccaatggcat tgttgccggg ccccgtggcg
   127801 cggcgacacg attagggatg aagcgcacca cgctgctgtc acgaatgcag cgtctgggaa
   127861 tctcggttcg cgaggtgttg taatctgctt ttgcaggagt atgcatgaga aacaaactct
   127921 ctttcgactt gcagttgagc gccagaaaag cggcaatcgc tgaacggatt gccgcccata
   127981 aaattgcccg cagtaaagtg tcggtctttt taatggcgat gtccgctggc gtgtttatgg
   128041 cgatcggatt tactttttac ctttccgtta tcgccgatgc cccgtcttca taggcattaa
   128101 cccatctggt gggcggcctt tgctttacac ttggctttat tttgctggcg gtttgcggca
   128161 ccagcctgtt cacctcgtcg gtaatgacgg taatggcaaa aagtcggggc gttattagtt
   128221 ggcgaacttg gctgattaac gcacttctgg tggcctgcgg taatctggca ggtattgcct
   128281 gtttcagttt gttaatctgg ttttccgggc tggtgatgag tgaaaacgcg atgtggggag
   128341 tcgcgatttt acactgcgcc gagggcaaaa tgcatcatac atttactgaa tctgtcagcc
   128401 tcggcattat gtgcaatctg atggtttgcc tggcgctgtg gatgagttat tgcggacgtt
   128461 cgttatgcga caaaatcgtc gccatgattt tgtccatcac cctgtttgtc gccagtggct
   128521 ttgagcactg tatcgccaat ttgtttgtga ttccgttcgc cattgccatt cgccatttcg
   128581 cccctacctc cttctggcaa ctggcgcaca gtagcgcaga caattttccg gcactgacgg
   128641 tcagccattt tattaccgcc aatctgctcc cggtgatgct gggtaatatt atcggcggtg
   128701 cagtgctggt gagtatttgt tatcgggcta tttatttacg tcaggaatcc tgaaaaatca
   128761 gcccggcgaa acagttcgtc gggctgaaga ttattcttgc gcgatttgcc catcgggcca
   128821 ggcgtggatc acggctttga tcagcgtcgc caatggaatg gcgaagaata cgccccagaa
   128881 tccccacaaa ccaccgaaga tcaccaccga taaaataatc accagcggat gcaggttaac
   128941 cgcttcggag aacaacaccg gtactagcag gttgccgtcc agcgcctgaa taatcagata
   129001 caccgcgaag cagctccaga attcagtacc tgcgccaaac tggaatagcg ccacgccaac
   129061 caccggaatg gtcaccacaa atgcgccaat gtacggaata agaaccgaga agccgaccag
   129121 cactgccagc agcagcgaat agttcagccc gaagagcaag aaccccagcc aggtggcgat
   129181 ccccaccacg atcatctcca gcactttgcc gcggatatag ttggtgattt gttgattcat
   129241 ctccttccac acctgtcctg ccagcccacg gttacgcggc agcacccggc gaacggcgtt
   129301 cagcatctgc tctttgtctt tcagcaggaa gaagaccatc aatggcacca gcaccagata
   129361 gacggctatg gtcagcaaac cgaccagcga ggcgagggaa attttcacca ccgaatcgcc
   129421 catggtcagc atccgactgc gcatattttc ggccattgca tcaataatgc ccgcgtccat
   129481 taacaccgga tagcggcgcg gcaacgtggc ggcaaagtca gaaagcttat tgagcatccc
   129541 cggcatatcg cggattaagt agatgccctg ttgccaggcg ataggcagta ccacgaacgc
   129601 catcagtagc aatataccga caaaaaccac caatacaatc gacgtcgccc agcggcggga
   129661 gcagccaata gattgcaggc gcacggttgg ccattccagc aaataagcca gcacaatagc
   129721 caccagcaac ggagcaagca ggccactaaa gaaaaagata atgccaaatc cggcaactaa
   129781 aataaccagc aaggcaatcg cttccgggtc gctaaaacgg cggcgatacc attgcatcaa
   129841 catttcgagc ataaagacct tccctgaacc tcaagagcgg gattgcgatc cgcaattgta
   129901 tcgaaatgtc acaaaaaaga cttcgctttt tatgacggat tcaggaaact gaaaagtcat
   129961 ttgagtgggc taatcttcgc cgttacactc aaaggcggcg aggtgggaac gatatttcac
   130021 agtatcggtc aaatgactat cttcagaaat acaggataga ggttatgttc aggcagttga
   130081 aaaaaaacct ggttgcaacc ctcattgctg ctatgaccat tggtcaggta gccccggcgt
   130141 ttgccgacag cgcagacacc ttgccggata tgggaacctc cgcaggaagc acgctttcca
   130201 ttggtcagga aatgcagatg ggcgactatt atgtccgcca gctacgcggc agcgcgccgt
   130261 taattaatga cccgctgtta acgcaatata ttaattcgct ggggatgcgt ctggtttcgc
   130321 atgccaattc ggttaagaca ccgtttcatt tttttctgat caacaacgac gaaattaacg
   130381 cctttgcttt ctttggcggc aacgtggtgc tgcactctgc cctgttccgt tattccgata
   130441 acgaaagtca actggcttca gttatggcgc acgaaatctc ccacgtcacc caacgtcacc
   130501 tggcgcgagc gatggaagat caacaacgca gcgcgccgct gacctgggtc ggcgcgttag
   130561 gttctatttt actggcgatg gccagtccgc aggcggggat ggcggcgctg accggtacac
   130621 tggcgggaac gcgccagggg atgatcagtt tcacccagca aaatgaacag gaagcggacc
   130681 gcattggtat tcaggtgctg caacgctcgg gattcgatcc acaggcgatg ccgaccttcc
   130741 tggaaaaatt gctcgatcag gcgcgttact cctcgcgccc gccggaaatt ttattgactc
   130801 acccgttgcc ggaaagtcgt ctggcagatg cccgcaaccg tgctaatcag atgcgcccga
   130861 tggtggtgca gtcgtcggaa gatttctatc tggcgaaagc gcgcacactg gggatgtata
   130921 attccggacg taaccagctc accagtaatt tgctggatga atgggcgaaa ggaaacgttc
   130981 ttcagcaacg agcggcgcaa tatggtcgtg ctttacaggc gatgaaagcc aataaatacg
   131041 acgaggcgcg taaaacgctg caaccgttac tggcggcaga acctggcaac gcatggtatc
   131101 tcgatctggc taccgatatc gatcttgagc aaaacaaagc caatgaggca atcaatcgcc
   131161 tgaaaaatgc ccgcgattta cgcaccaatc cggtgttgca gctcaacctg gcgaacgctt
   131221 atctgcaagg cggtcaacca caagaagcgg ccaatattct gaatcgctac acctttaata
   131281 ataaagatga cagcaacggc tgggatttgc tggcacaggc ggaagccgcg ctaaataacc
   131341 gcgatcagga gctggctgcg cgagcagaag gttatgcgct cgccggacga ctcgatcagg
   131401 ccatttcgct gttgagtagc gccagttcgc aggtgaaatt aggcagcctg caacaagcgc
   131461 gttacgatgc gcgcatcgac cagttgcgcc agctgcagga acgctttaag ccttatacca
   131521 agatgtaatt cgcattaaag gaagaacaat gaccaaacag gtaaaaatct accataaccc
   131581 acgctgttca aagagccggg aaacgctgaa cctgctgaaa gaaaacggcg tggagccaga
   131641 agtggtgctc taccttgaga cacccgccga tgcggcaacg ctgcgcgatt tgctgaaaat
   131701 gctggggatg aatagcgccc gtgaattgat gcgccagaaa gaggatcttt ataaagaact
   131761 aaacctggct gacagttcgc ttagcgaaga ggcgctgatt caggcaatgg tggataatcc
   131821 gaagctgatg gagcgcccga ttatggtcgc gaatggcaaa gcgcggattg gtcgtccacc
   131881 ggagcaggta ctggaaatcg tgggttaaga tgtgctccat cgctagttga agcacattgc
   131941 cggatgcgac gcttgccacg tcttatccgg cctacgaata ctatggatta acttcgcgta
   132001 ggtcaggcgt tcgcgccgca tccgacaata aacaccttat ctacaacttc agaatttctt
   132061 tcacaaacgg aatggtcagc ttacgttgcg cggtaatcga cgcacgatcc aactgatcca
   132121 acgtcataaa tagcgtgcgc atttctctgt cgagccgctt cagcaagaaa cgccccacat
   132181 cttccggcag ttcaaaacca cgcaaacgcg cgcgtaactg tagcgcctgc aacttatctt
   132241 catcagaaag tggctgcaat ttgtagatct gcccccagtc gagacgcgac gcgagatccg
   132301 gtaatcccag attcaactgc cgcggtggac gatcgccggt gatcaacaac cgtgttttgc
   132361 ccgattccag aattcgattg tagagatcga aaatcgccat ctcccacaac tcatcgcctg
   132421 caatacactc aatgttgtcg atacagacca gcgacaaatg ctccataccg tcgagcactt
   132481 ccggaacaaa ccaggtgcgt ttatccagcg ggacatagcc caccgcatcg ccacgctgcg
   132541 acaattccgc gcaagccgcg tgcagcagat ggctgcgccc cgcgccttcg cgtgcccaga
   132601 gatagatgta accgctatgt tcctgacgca gcacgttttg cagcgcggcc agtaaagagg
   132661 agttatcccc cggccagaaa cttgcaaagg tttcgtcgtc aggaagataa agtggcaaag
   132721 agagctgtgc cggtgtgttc agagatacct caaccaggat ttcacaaaat cgcgagaagt
   132781 ttaccacaga atcccatgat gtttgaaccg ggcagcaaca ctgcccggtc ggtacattat
   132841 ttgtctgtta tatccgcgtc ttctgcgtcc agcaccactt cttcagggcg cagcacactg
   132901 atcagtttga aaatcaaact taagccgata ccgacgatgg tcgccagcgc catacctttt
   132961 aactccgccg cgccgatgtt taccttcgcg ccactgacgc cgatgatcaa aatcacggaa
   133021 gtcaggatca ggttctgtgc tttgttgtaa tccactttcg attcgatcaa aacacgaata
   133081 ccggaagcac caatgacacc ataaagcagc agcgaaacgc cgcccatcac cggcaatggg
   133141 atcatctgga tagcggcagc cagtttaccg acgcaggaaa gcaggatagc gaaaatcgcc
   133201 gccccgccga taacccaggt actgtaaaca cgggtgatcg ccatcacgcc aatgttttct
   133261 ccgtaagtgg tatttggcgt agagccaaag aagccggaga tcacggtcga caggccgtta
   133321 gcaaacatcg aacggtgcag acctggatcg cgcagcagat cttttttgac gatattagcc
   133381 gttactacca ggtgccctac gtgttcggca ataaccacta acgccgctgg cagaatagtc
   133441 agaatggcaa accactcgaa gcgcggcgta tagagggttg gcagtgcaaa ccagtgagca
   133501 ttaataatcg gcgtggtatc gacaattccc attgcgaaag agagcgcgta ccccaccagc
   133561 acgccaatta aaatcgggat aattgccagg aaaccacgaa acagcacgga acctaaaacc
   133621 gtgaccgcca gggtggtaat agagatgatg atggttttgg agtttggcgt ttgcccttca
   133681 gccgggagta aacccgccat accggcagct acgcccgcca gctccagacc gatgacggca
   133741 acgattgcgc ccattgccgc aggtggaaac agcacgtcca accagccggt ccccgctttc
   133801 ttcacgataa aagaaaccag gcagaacagc acgccgcaca taataaagcc gcccagcgcg
   133861 acttcatacc ctaacggcaa cagtaacaat accggtgaaa taaaggcaaa gctggaacca
   133921 agataagccg gaattttccc tttacagatg aagagataca gcagcgttcc aataccgtta
   133981 aataacagta cagtcgccgg gttaatatga aataagacgg gcaccaggac ggttgcacca
   134041 aacatggcga acaaatgttg caaactaagc gggattgtct gtaaaagtgg cggtctttca
   134101 ctcaccccga tagcacggcg cgtcatagta ttatcctctg tattatgtgt tataggcgct
   134161 ttactcaaaa aaaagccgac tcttaaagtc ggctttaatt atttttattc tttatttcgt
   134221 accaaagatt ttgtcaccgg catcgccgag gcccggaata atgtatccgt gctcgttcag
   134281 tccctggtca atcgatgcag tatacagttc gacgtccggg tgcgcttttt ccagcgcagc
   134341 gataccttct ggcgcagcga ccagcaccag aactttgatg ctgctgcagc ccgctttttt
   134401 cagcaggtcg atggtcgcga taacggaacc accggttgcc agcattgggt caacgatcag
   134461 cgccatacgc tcatcgatgt tagaaaccag tttctggaag tacggtaccg gctccagcgt
   134521 ttcttcatta cggtacatac cgacaacgct gatgcgcgcg ctcggaacgt tttccagcac
   134581 accgtccatc ataccaagac ccgcacgcag aattggcaca acggtaattt tcttaccttt
   134641 gatctggtcg atttctaccg ggccgttcca gccttcgata gttacttttt ccgtttcgag
   134701 gtcggcggtc gcttcgtaag tcagcaggct acccacttcg gaagcgagtt cgcgaaagcg
   134761 cttggtgctg atatcttgct cacgcatcag tcccagcttg tgtttgacga gtgggtgttt
   134821 gacttccacg atcttcatac tctttctcct ttgaggggca gccacaaaaa aaatcgaagg
   134881 attatacctc ctttcttcaa ggcggcaata ttattttcgt tgactttagt caaaatgata
   134941 acagtttgag ataaagttat tttatattca aatggttatg aaagaagatt attccatccg
   135001 aaaactaacc tttgccctgg cacaagtctt ctttcgccgc gcgcccgggg aaaagacgtg
   135061 caaaaaggtt gtgtaaaaca gtctcgcaaa cgtttgcttt ccctgttaga attgcgccga
   135121 attttatttt tctaccgcaa gtaacgcgtg gggacccaag cagtgaccga taaaacctct
   135181 cttagctaca aagatgccgg tgttgatatt gacgcgggta atgctctggt tggaagaatc
   135241 aaaggcgtag tgaagaaaac gcgtcgtccg gaagtgatgg gcggtctggg cggcttcggt
   135301 gcgctgtgtg cattgccgca aaaatatcgt gaacccgtgc tggtttccgg cactgacggc
   135361 gtaggtacca agctgcgtct ggcaatggac ttaaaacgtc acgacaccat tggtattgat
   135421 ctggtcgcca tgtgcgttaa tgacctggtg gtgcaaggtg cggaaccgct gtttttcctc
   135481 gactattacg caaccggaaa actggatgtt gataccgctt cagcagtgat cagcggcatc
   135541 gcggaaggtt gtctgcaatc gggctgttct ctggtgggtg gcgaaacggc agaaatgccg
   135601 gggatgtatc acggtgaaga ttacgatgtc gcgggtttct gcgtgggcgt ggtagaaaaa
   135661 tcagaaatca tcgacggctc taaagtcagc gacggcgatg tgctgattgc actcggttcc
   135721 agcggtccgc actcgaacgg ctattcgctg gtgcgcaaaa ttcttgaagt cagcggttgt
   135781 gatccgcaaa ccaccgaact tgatggtaag ccattagccg atcatctgct ggcaccgacc
   135841 cgcatttacg tgaagtcagt gctggagttg attgaaaagg tcgatgtgca tgccattgcg
   135901 cacctgaccg gcggcggctt ctgggaaaac attccgcgcg tattgccaga taatacccag
   135961 gcagtgattg atgaatcttc ctggcagtgg ccggaagtgt tcaactggct gcaaacggca
   136021 ggtaacgttg agcgccatga aatgtatcgc accttcaact gcggcgtcgg gatgattatc
   136081 gccctgcctg ctccggaagt ggacaaagcc ctcgccctgc taaatgccaa cggtgaaaac
   136141 gcgtggaaaa tcggtatcat caaagcctct gattccgaac aacgcgtggt tatcgaataa
   136201 tgaatattgt ggtgcttatt tccggcaacg gaagtaattt acaggcaatt attgacgcct
   136261 gtaaaaccaa caaaattaaa ggcaccgtac gggcagtttt cagcaataag gccgacgcgt
   136321 tcggccttga acgcgcccgc caggcgggta ttgcaacgca tacgctcatc gccagcgcgt
   136381 ttgacagtcg tgaagcctat gaccgggagt tgattcatga aatcgacatg tacgcacccg
   136441 atgtggtcgt gctggctggt tttatgcgca ttctcagccc ggcgtttgtc tcccactatg
   136501 ccgggcgttt gctgaacatt cacccttctc tgctgccgaa atatcccgga ttacacaccc
   136561 atcgtcaggc gctggaaaat ggcgatgaag agcacggtac atcggtgcat ttcgtcaccg
   136621 atgaactgga cggtggcccg gttattttac aggcgaaagt cccggtattt gctggtgata
   136681 cggaagatga catcaccgcc cgcgtgcaaa cccaggaaca cgctatttat ccactggtga
   136741 ttagctggtt tgccgatggt cgtctgaaaa tgcacgaaaa cgccgcgtgg ctggatggtc
   136801 aacgtctgcc gccgcagagc tacgctgccg acgagtaatg cccccgtagt taaagcgcca
   136861 gctctgccgc tggcgttttt caattcacct gtaaatcgca agctccagca gtttttttcc
   136921 cccttttctg gcatagttgg acatctgcca atattgctcg ccataatatc caggcagtgt
   136981 cccgtgaata aaacggagta aaagtggtaa tgggtcagga aaagctatac atcgaaaaag
   137041 agctcagttg gttatcgttc aatgaacgcg tgcttcagga agcggcggac aaatctaacc
   137101 cgctgattga aaggatgcgt ttcctgggga tctattccaa taaccttgat gagttctata
   137161 aagtccgctt cgctgaactg aagcgacgca tcattattag cgaagaacaa ggctccaacg
   137221 ctcattcccg ccatttactg ggcaaaattc agtcccgggt gctgaaagcc gatcaggaat
   137281 tcgacggcct ctacaacgag ctattgctgg agatggcgcg caaccagatc ttcctgatta
   137341 atgaacgcca gctctccgtc aatcaacaaa actggctgcg tcattatttt aagcagtatc
   137401 tgcgtcagca cattacgccg attttaatca atcctgacac tgacttagtg cagttcctga
   137461 aagatgatta cacctatctg gcggtggaaa ttatccgtag cgatagcatc cgttacgcgc
   137521 tgctggagat cccatcagat aaagtgccgc gctttgtgaa tttaccgcca gaagcgccgc
   137581 gtcgacgcaa gccgatgatt cttctggata acattctgcg ttactgcctt gatgatattt
   137641 tcaaaggctt ctttgattat gacgcgctga atgcctattc aatgaagatg acccgcgatg
   137701 ccgaatacga tttagtgcat gagatggaag ccagcctgat ggagttgatg tcttccagtc
   137761 tcaagcagcg tttaactgct gagccggtgc gttttgttta tcagcgcgat atgcccaatg
   137821 cgctggttga agtgttacgc gaaaaactga ctatttcccg ctacgactcc atcgtccccg
   137881 gcggtcgtta tcataatttt aaagacttta ttaattttcc caatgtcggc aaagccaatc
   137941 tggtgaacaa accactgccg cgtttacgcc atatttggtt tgataaagcc cagttccgca
   138001 atggttttga tgccattcgc gaacgcgatg tgttgctcta ttatccttat cacacctttg
   138061 ggcatgtgct ggaactgctg cgtcaggctt cgttcgaccc gagcgtactg gcgattaaaa
   138121 ttaacattta ccgcgtggcg aaagattcac gcatcatcga ctcgatgatc cacgccgcac
   138181 ataacggtaa gaaagtcact gtggtggttg agttacaggc gcgtttcgac gaagaagcca
   138241 acattcactg ggcgaagcgc ctgaccgaag caggcgtgca cgttatcttc tctgcgccgg
   138301 ggctgaaaat tcacgccaaa ctgttcctga tttcacgtaa agaaaacggt gaagtggtcc
   138361 gttacgcaca catcgggacc gggaacttta acgaaaaaac cgcgcgtctt tatactgact
   138421 attcgttgct gaccgcagat gcgcgtatca ccaacgaagt acggcgggta tttaacttta
   138481 ttgaaaaccc ataccgcccg gtgacatttg attatttaat ggtgtcaccg caaaactctc
   138541 gccgtctgtt atatgaaatg gtagaccgcg aaatcgccaa cgcgcagcaa gggctgccca
   138601 gtggtatcac cctgaagcta aataaccttg tcgataaagg cctggttgat cgtctgtatg
   138661 cggcctccag ctccggcgta ccggttaatc tgctggttcg cggaatgtgt tcgctgatcc
   138721 ccaatctgga aggcattagc gacaacattc gtgccatcag tattgttgac cgttaccttg
   138781 aacatgaccg ggtttatatt tttgaaaatg gcggcgataa aaaggtctac ctttcttccg
   138841 ccgactggat gacgcgcaat attgattatc gtattgaagt ggcgacaccg ctgctcgatc
   138901 cgcgcctgaa gcagcgggtg ctggacatca tcgacatatt gttcagcgat acggtcaaag
   138961 cacgttatat cgataaagaa ctcagtaatc gctacgttcc ccgcggcaat cgccgcaaag
   139021 tacgggcgca gttggcgatt tacgactaca tcaaatcact cgaacaacct gaataaccct
   139081 atgccaatac acgataaatc ccctcgtccg caggagtttg ctgcggtcga tcttggttca
   139141 aacagttttc acatggtcat agcccgtgtg gtagatggtg ccatgcagat tattggccgc
   139201 ctgaaacagc gggtgcatct ggcggacggc ctggggccag ataatatgtt gagtgaagag
   139261 gcaatgacgc gcggtttgaa ctgtctgtcg ctgtttgccg aacggctaca agggttttct
   139321 cctgccagcg tctgtatagt cggcacccat acgctgcgtc aggcgctgaa cgccactgac
   139381 tttctgaaac gcgcggaaaa ggtcattccc tacccgattg aaattatttc cggtaatgaa
   139441 gaagcccgtc tgatttttat gggcgtggaa catacccaac cggaaaaagg tcgcaaactg
   139501 gttattgata ttggcggcgg atctacggaa ctggtgattg gtgaaaattt cgaacctatt
   139561 ctcgttgaaa gccgccggat gggttgtgtc agctttgccc agctttattt tcctggcggg
   139621 gtcatcaata aagagaattt tcagcgcgct cgcatggcgg cagcacaaaa actggaaact
   139681 ttaacctggc aattccgtat tcagggctgg aacgttgcaa tgggcgcttc cggtaccata
   139741 aaagccgccc atgaagtgtt aatggaaatg ggcgagaaag acgggataat taccccggaa
   139801 cgtctggaaa aactggtaaa agaaatttta cgtcaccgta atttcgcatc gctgagttta
   139861 ccgggtcttt ccgaagagcg gaaaacagtc ttcgttcctg gactggcgat tttatgtggt
   139921 gtgtttgatg ctttagccat ccgtgaactg cgcctttctg acggggcgtt acgcgaaggc
   139981 gtactgtatg aaatggaagg acgtttccgt catcaggatg tgcgtagtcg caccgccagc
   140041 agcctcgcca accagtatca catagacagc gaacaggccc gacgagtgct ggataccact
   140101 atgcaaatgt acgaacagtg gcgggaacag caaccgaagc tggcgcatcc gcaactggag
   140161 gcgctactgc gatgggccgc catgctgcat gaggtcgggt tgaatatcaa ccacagcggt
   140221 ttgcatcgcc actccgctta tattctgcaa aacagtgact tgccgggttt taatcaggaa
   140281 cagcagctga tgatggcgac actgctgcgc tatcaccgta aagcgattaa gctcgacgat
   140341 ctgccgcgct ttaccttgtt taagaagaaa cagttcctgc cactgataca gctattgcgc
   140401 cttggcgtat tactaaacaa tcaacgtcag gcaaccacca caccgtcaac attgacactg
   140461 attactgatg acagtcactg gacactgcgt ttcccgcatg actggtttag tcagaatgcg
   140521 ctggtactgc ttgatctgga aaaggagcaa gaatactggg aaggcgtggc tggctggcgg
   140581 ttgaaaattg aagaagaaag taccccagaa atcgccgctt aatgctttgc gggccgacga
   140641 gaaatgtcgg cccgcattat tcaggcactt tcgcgaatgg gttcgatttc attcagcgta
   140701 tcaattaacg gttgcggctt accaataaga taaccctgca tataatcgat ccccaaagag
   140761 agcaccgcct cgcggatctc ttcgttttca acgtactctg ccactacccg cattttcttc
   140821 attcgcgcca gatggcaaat cgacgccaca atctgataat ccagactatt tgacacaata
   140881 ttgcggataa agctgccgtc aattttaagc agatcggcat tcacattttt aagccgtgca
   140941 tagctggcgt agccggtgcc gaaatcatca atcgcaatct ggcagcctaa ttcctgaaga
   141001 tgctgcaagg tgatttgcgc ctgcttaaca ttggtcagag cattactttc ggtgacttca
   141061 aaaataagtt gccacgcttc aatctgatat ttagccagca actgactgac ttcaacagga
   141121 aaacgagcct gacataccga ggttggagac agattaatag caaaacggtg agcgggcatc
   141181 ttcgctctgt tttcagccat aaattgcagc gtatgctcaa tgacccacat gtcgatactc
   141241 gacgataaac caaattcgtg cgcgaccggc aagaagctat cggggccgat cagttcatca
   141301 ttctcacctt tcatgcgcag aagaatttca tggtaaacat caccacgcat accggtaatc
   141361 ggctgggcca tcaggaaaaa atggttgtgc tccagcgcct gctgtagtcg attcatcatc
   141421 gcgactttat ctttcaattc gcgttgcaaa tacattgccc cgcaacgctg catattttcc
   141481 ggggcgttgg tcacgatgga aagttcggcg accgtattta gctctcccag cagcaggtag
   141541 atatgattca ctggcgagcg cacatagcag taactgacgc caatctgcgg ttgcatcggc
   141601 atcccatccc aaaagaaacg aaattgcttg agatgactat ccagtgcggt aatgcgctcc
   141661 tggtgcgatt ctgtattcag tcgcagcgcg agatcgttac ccgaaagctg ataaacatct
   141721 tcacccggtt ccagcaaggg tgacagccag tgagaaagtt tttgcttgt
//
LOCUS       DAJTXY010000002       111328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTXY010000002 DAJTXY010000000
VERSION     DAJTXY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 111328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 111328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.002"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            200..2485
                     /gene="nrdA"
                     /locus_tag="OO566_000134"
     CDS             200..2485
                     /gene="nrdA"
                     /locus_tag="OO566_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6603560.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2719..3849
                     /gene="nrdB"
                     /locus_tag="OO566_000135"
     CDS             2719..3849
                     /gene="nrdB"
                     /locus_tag="OO566_000135"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6603561.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            3849..4103
                     /gene="yfaE"
                     /locus_tag="OO566_000136"
     CDS             3849..4103
                     /gene="yfaE"
                     /locus_tag="OO566_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6603562.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4157..4807)
                     /gene="inaA"
                     /locus_tag="OO566_000137"
     CDS             complement(4157..4807)
                     /gene="inaA"
                     /locus_tag="OO566_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6603563.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            4971..5213
                     /locus_tag="OO566_000138"
                     /pseudo
     CDS             4971..5213
                     /locus_tag="OO566_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5255..6331)
                     /gene="glpQ"
                     /locus_tag="OO566_000139"
     CDS             complement(5255..6331)
                     /gene="glpQ"
                     /locus_tag="OO566_000139"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6603564.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6336..7694)
                     /gene="glpT"
                     /locus_tag="OO566_000140"
     CDS             complement(6336..7694)
                     /gene="glpT"
                     /locus_tag="OO566_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6603565.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            7967..9595
                     /gene="glpA"
                     /locus_tag="OO566_000141"
     CDS             7967..9595
                     /gene="glpA"
                     /locus_tag="OO566_000141"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6603566.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9585..10844
                     /gene="glpB"
                     /locus_tag="OO566_000142"
     CDS             9585..10844
                     /gene="glpB"
                     /locus_tag="OO566_000142"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6603567.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            10841..12031
                     /gene="glpC"
                     /locus_tag="OO566_000143"
     CDS             10841..12031
                     /gene="glpC"
                     /locus_tag="OO566_000143"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6603568.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12224..13123
                     /locus_tag="OO566_000144"
     CDS             12224..13123
                     /locus_tag="OO566_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6603569.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13127..13363
                     /locus_tag="OO566_000145"
                     /pseudo
     CDS             13127..13363
                     /locus_tag="OO566_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13404..13874)
                     /locus_tag="OO566_000146"
     CDS             complement(13404..13874)
                     /locus_tag="OO566_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6603570.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(13929..15131)
                     /locus_tag="OO566_000147"
     CDS             complement(13929..15131)
                     /locus_tag="OO566_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6603571.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSSRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15218..15760)
                     /locus_tag="OO566_000148"
     CDS             complement(15218..15760)
                     /locus_tag="OO566_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603572.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16039..16464
                     /gene="nudI"
                     /locus_tag="OO566_000149"
     CDS             16039..16464
                     /gene="nudI"
                     /locus_tag="OO566_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6603573.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16503..17105)
                     /locus_tag="OO566_000150"
     CDS             complement(16503..17105)
                     /locus_tag="OO566_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6603574.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNMVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17413..18552
                     /gene="arnB"
                     /locus_tag="OO566_000151"
     CDS             17413..18552
                     /gene="arnB"
                     /locus_tag="OO566_000151"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6603575.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18556..19524
                     /gene="arnC"
                     /locus_tag="OO566_000152"
     CDS             18556..19524
                     /gene="arnC"
                     /locus_tag="OO566_000152"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6603576.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19524..21506
                     /gene="arnA"
                     /locus_tag="OO566_000153"
     CDS             19524..21506
                     /gene="arnA"
                     /locus_tag="OO566_000153"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6603577.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDFVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21503..22393
                     /locus_tag="OO566_000154"
     CDS             21503..22393
                     /locus_tag="OO566_000154"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6603578.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22393..24045
                     /gene="arnT"
                     /locus_tag="OO566_000155"
     CDS             22393..24045
                     /gene="arnT"
                     /locus_tag="OO566_000155"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6603579.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDLWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24042..24377
                     /gene="arnE"
                     /locus_tag="OO566_000156"
     CDS             24042..24377
                     /gene="arnE"
                     /locus_tag="OO566_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6603580.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24377..24763
                     /gene="arnF"
                     /locus_tag="OO566_000157"
     CDS             24377..24763
                     /gene="arnF"
                     /locus_tag="OO566_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6603581.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24757..25029)
                     /gene="pmrD"
                     /locus_tag="OO566_000158"
     CDS             complement(24757..25029)
                     /gene="pmrD"
                     /locus_tag="OO566_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6603582.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25134..26489)
                     /gene="menE"
                     /locus_tag="OO566_000159"
     CDS             complement(25134..26489)
                     /gene="menE"
                     /locus_tag="OO566_000159"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6603583.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADVLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26486..27448)
                     /gene="menC"
                     /locus_tag="OO566_000160"
     CDS             complement(26486..27448)
                     /gene="menC"
                     /locus_tag="OO566_000160"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6603584.1"
                     /translation="MRSAQVYRWQIPMDAGVLLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27448..28305)
                     /gene="menB"
                     /locus_tag="OO566_000161"
     CDS             complement(27448..28305)
                     /gene="menB"
                     /locus_tag="OO566_000161"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6603585.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28320..29078)
                     /gene="menH"
                     /locus_tag="OO566_000162"
     CDS             complement(28320..29078)
                     /gene="menH"
                     /locus_tag="OO566_000162"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6603586.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29075..30745)
                     /gene="menD"
                     /locus_tag="OO566_000163"
     CDS             complement(29075..30745)
                     /gene="menD"
                     /locus_tag="OO566_000163"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6603587.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30834..32129)
                     /gene="menF"
                     /locus_tag="OO566_000164"
     CDS             complement(30834..32129)
                     /gene="menF"
                     /locus_tag="OO566_000164"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6603588.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32208..32513)
                     /gene="elaB"
                     /locus_tag="OO566_000165"
     CDS             complement(32208..32513)
                     /gene="elaB"
                     /locus_tag="OO566_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6603589.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32568..33029)
                     /locus_tag="OO566_000166"
     CDS             complement(32568..33029)
                     /locus_tag="OO566_000166"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6603590.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33094..34011
                     /gene="rbn"
                     /locus_tag="OO566_000167"
     CDS             33094..34011
                     /gene="rbn"
                     /locus_tag="OO566_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6603591.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TAQRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34199..35419
                     /gene="elaD"
                     /locus_tag="OO566_000168"
     CDS             34199..35419
                     /gene="elaD"
                     /locus_tag="OO566_000168"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR6603592.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGNEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSELMGKMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
     gene            35839..36454
                     /locus_tag="OO566_000169"
                     /pseudo
     CDS             35839..36454
                     /locus_tag="OO566_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            36557..37060
                     /locus_tag="OO566_000170"
     CDS             36557..37060
                     /locus_tag="OO566_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCR6603593.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(37127..38584)
                     /gene="nuoN"
                     /locus_tag="OO566_000171"
     CDS             complement(37127..38584)
                     /gene="nuoN"
                     /locus_tag="OO566_000171"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6603594.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFDYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(38591..40120)
                     /gene="nuoM"
                     /locus_tag="OO566_000172"
     CDS             complement(38591..40120)
                     /gene="nuoM"
                     /locus_tag="OO566_000172"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6603595.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(40284..42125)
                     /gene="nuoL"
                     /locus_tag="OO566_000173"
     CDS             complement(40284..42125)
                     /gene="nuoL"
                     /locus_tag="OO566_000173"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6603596.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAAQV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(42122..42424)
                     /gene="nuoK"
                     /locus_tag="OO566_000174"
     CDS             complement(42122..42424)
                     /gene="nuoK"
                     /locus_tag="OO566_000174"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6603597.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(42421..42975)
                     /gene="nuoJ"
                     /locus_tag="OO566_000175"
     CDS             complement(42421..42975)
                     /gene="nuoJ"
                     /locus_tag="OO566_000175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6603598.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(42987..43529)
                     /gene="nuoI"
                     /locus_tag="OO566_000176"
     CDS             complement(42987..43529)
                     /gene="nuoI"
                     /locus_tag="OO566_000176"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6603599.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(43544..44521)
                     /gene="nuoH"
                     /locus_tag="OO566_000177"
     CDS             complement(43544..44521)
                     /gene="nuoH"
                     /locus_tag="OO566_000177"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6603600.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(44518..47244)
                     /gene="nuoG"
                     /locus_tag="OO566_000178"
     CDS             complement(44518..47244)
                     /gene="nuoG"
                     /locus_tag="OO566_000178"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6603601.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(47297..48634)
                     /gene="nuoF"
                     /locus_tag="OO566_000179"
     CDS             complement(47297..48634)
                     /gene="nuoF"
                     /locus_tag="OO566_000179"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6603602.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(48631..49131)
                     /gene="nuoE"
                     /locus_tag="OO566_000180"
     CDS             complement(48631..49131)
                     /gene="nuoE"
                     /locus_tag="OO566_000180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6603603.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(49134..50936)
                     /gene="nuoC"
                     /locus_tag="OO566_000181"
     CDS             complement(49134..50936)
                     /gene="nuoC"
                     /locus_tag="OO566_000181"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6603604.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(51030..51692)
                     /gene="nuoB"
                     /locus_tag="OO566_000182"
     CDS             complement(51030..51692)
                     /gene="nuoB"
                     /locus_tag="OO566_000182"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6603605.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(51708..52151)
                     /gene="nuoA"
                     /locus_tag="OO566_000183"
     CDS             complement(51708..52151)
                     /gene="nuoA"
                     /locus_tag="OO566_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6603606.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(52782..53720)
                     /gene="lrhA"
                     /locus_tag="OO566_000184"
     CDS             complement(52782..53720)
                     /gene="lrhA"
                     /locus_tag="OO566_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6603607.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            54684..55901
                     /gene="alaA"
                     /locus_tag="OO566_000185"
     CDS             54684..55901
                     /gene="alaA"
                     /locus_tag="OO566_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6603608.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            55985..56584
                     /gene="yfbR"
                     /locus_tag="OO566_000186"
     CDS             55985..56584
                     /gene="yfbR"
                     /locus_tag="OO566_000186"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6603609.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(56643..58475)
                     /locus_tag="OO566_000187"
     CDS             complement(56643..58475)
                     /locus_tag="OO566_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6603610.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(58562..59212)
                     /gene="hxpA"
                     /locus_tag="OO566_000188"
     CDS             complement(58562..59212)
                     /gene="hxpA"
                     /locus_tag="OO566_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6603611.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALSGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(59223..59717)
                     /locus_tag="OO566_000189"
     CDS             complement(59223..59717)
                     /locus_tag="OO566_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6603612.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(59800..60255)
                     /locus_tag="OO566_000190"
     CDS             complement(59800..60255)
                     /locus_tag="OO566_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6603613.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            60593..61795
                     /gene="ackA"
                     /locus_tag="OO566_000191"
     CDS             60593..61795
                     /gene="ackA"
                     /locus_tag="OO566_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6603614.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            61870..64014
                     /gene="pta"
                     /locus_tag="OO566_000192"
     CDS             61870..64014
                     /gene="pta"
                     /locus_tag="OO566_000192"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6603615.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            64204..65724
                     /gene="yfcC"
                     /locus_tag="OO566_000193"
     CDS             64204..65724
                     /gene="yfcC"
                     /locus_tag="OO566_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6603616.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(65757..66299)
                     /gene="yfcD"
                     /locus_tag="OO566_000194"
     CDS             complement(65757..66299)
                     /gene="yfcD"
                     /locus_tag="OO566_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6603617.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(66357..66908)
                     /gene="yfcE"
                     /locus_tag="OO566_000195"
     CDS             complement(66357..66908)
                     /gene="yfcE"
                     /locus_tag="OO566_000195"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6603618.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(66964..67608)
                     /gene="yfcF"
                     /locus_tag="OO566_000196"
     CDS             complement(66964..67608)
                     /gene="yfcF"
                     /locus_tag="OO566_000196"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6603619.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            67744..68391
                     /gene="yfcG"
                     /locus_tag="OO566_000197"
     CDS             67744..68391
                     /gene="yfcG"
                     /locus_tag="OO566_000197"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6603620.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            68448..68810
                     /gene="folX"
                     /locus_tag="OO566_000198"
     CDS             68448..68810
                     /gene="folX"
                     /locus_tag="OO566_000198"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6603621.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            68831..69724
                     /locus_tag="OO566_000199"
     CDS             68831..69724
                     /locus_tag="OO566_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6603622.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(69772..70662)
                     /gene="rpnB"
                     /locus_tag="OO566_000200"
     CDS             complement(69772..70662)
                     /gene="rpnB"
                     /locus_tag="OO566_000200"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6603623.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIA
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(70859..71632)
                     /gene="hisP"
                     /locus_tag="OO566_000201"
     CDS             complement(70859..71632)
                     /gene="hisP"
                     /locus_tag="OO566_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6603624.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEACERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(71640..72356)
                     /locus_tag="OO566_000202"
     CDS             complement(71640..72356)
                     /locus_tag="OO566_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6603625.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(72353..73039)
                     /gene="hisQ"
                     /locus_tag="OO566_000203"
     CDS             complement(72353..73039)
                     /gene="hisQ"
                     /locus_tag="OO566_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6603626.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(73129..73911)
                     /gene="hisJ"
                     /locus_tag="OO566_000204"
     CDS             complement(73129..73911)
                     /gene="hisJ"
                     /locus_tag="OO566_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6603627.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(74132..74914)
                     /gene="argT"
                     /locus_tag="OO566_000205"
     CDS             complement(74132..74914)
                     /gene="argT"
                     /locus_tag="OO566_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6603628.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(75180..75749)
                     /gene="ubiX"
                     /locus_tag="OO566_000206"
     CDS             complement(75180..75749)
                     /gene="ubiX"
                     /locus_tag="OO566_000206"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6603629.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(75844..77361)
                     /gene="purF"
                     /locus_tag="OO566_000207"
     CDS             complement(75844..77361)
                     /gene="purF"
                     /locus_tag="OO566_000207"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6603630.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(77398..77886)
                     /gene="cvpA"
                     /locus_tag="OO566_000208"
     CDS             complement(77398..77886)
                     /gene="cvpA"
                     /locus_tag="OO566_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6603631.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(78145..78807)
                     /gene="dedD"
                     /locus_tag="OO566_000209"
     CDS             complement(78145..78807)
                     /gene="dedD"
                     /locus_tag="OO566_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6603632.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(78797..80065)
                     /gene="folC"
                     /locus_tag="OO566_000210"
     CDS             complement(78797..80065)
                     /gene="folC"
                     /locus_tag="OO566_000210"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6603633.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(80135..81049)
                     /gene="accD"
                     /locus_tag="OO566_000211"
     CDS             complement(80135..81049)
                     /gene="accD"
                     /locus_tag="OO566_000211"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6603634.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(81205..81864)
                     /locus_tag="OO566_000212"
     CDS             complement(81205..81864)
                     /locus_tag="OO566_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6603635.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(81947..82759)
                     /gene="truA"
                     /locus_tag="OO566_000213"
     CDS             complement(81947..82759)
                     /gene="truA"
                     /locus_tag="OO566_000213"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6603636.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(82759..83772)
                     /locus_tag="OO566_000214"
     CDS             complement(82759..83772)
                     /locus_tag="OO566_000214"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6603637.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(83838..84974)
                     /gene="pdxB"
                     /locus_tag="OO566_000215"
     CDS             complement(83838..84974)
                     /gene="pdxB"
                     /locus_tag="OO566_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6603638.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            85073..86068
                     /gene="flk"
                     /locus_tag="OO566_000216"
     CDS             85073..86068
                     /gene="flk"
                     /locus_tag="OO566_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6603639.1"
                     /translation="MIQPISGPPLGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(86065..87243)
                     /locus_tag="OO566_000217"
     CDS             complement(86065..87243)
                     /locus_tag="OO566_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6603640.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLCFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(87518..88738)
                     /gene="fabB"
                     /locus_tag="OO566_000218"
     CDS             complement(87518..88738)
                     /gene="fabB"
                     /locus_tag="OO566_000218"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6603641.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            88897..90903
                     /gene="mnmC"
                     /locus_tag="OO566_000219"
     CDS             88897..90903
                     /gene="mnmC"
                     /locus_tag="OO566_000219"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6603642.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(91024..91302)
                     /locus_tag="OO566_000220"
     CDS             complement(91024..91302)
                     /locus_tag="OO566_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6603643.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(91336..91884)
                     /gene="epmC"
                     /locus_tag="OO566_000221"
     CDS             complement(91336..91884)
                     /gene="epmC"
                     /locus_tag="OO566_000221"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6603644.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(91884..92693)
                     /locus_tag="OO566_000222"
     CDS             complement(91884..92693)
                     /locus_tag="OO566_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6603645.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(92693..93517)
                     /gene="mepA"
                     /locus_tag="OO566_000223"
     CDS             complement(92693..93517)
                     /gene="mepA"
                     /locus_tag="OO566_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6603646.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(93521..94606)
                     /gene="aroC"
                     /locus_tag="OO566_000224"
     CDS             complement(93521..94606)
                     /gene="aroC"
                     /locus_tag="OO566_000224"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6603647.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(94641..95573)
                     /gene="prmB"
                     /locus_tag="OO566_000225"
     CDS             complement(94641..95573)
                     /gene="prmB"
                     /locus_tag="OO566_000225"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6603648.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            95739..96290
                     /gene="smrB"
                     /locus_tag="OO566_000226"
     CDS             95739..96290
                     /gene="smrB"
                     /locus_tag="OO566_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6603649.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(96394..97284)
                     /locus_tag="OO566_000227"
     CDS             complement(96394..97284)
                     /locus_tag="OO566_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6603650.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(97349..97795)
                     /locus_tag="OO566_000228"
     CDS             complement(97349..97795)
                     /locus_tag="OO566_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6603651.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNT"
     gene            complement(97792..98262)
                     /locus_tag="OO566_000229"
     CDS             complement(97792..98262)
                     /locus_tag="OO566_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6603652.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(98259..98807)
                     /locus_tag="OO566_000230"
     CDS             complement(98259..98807)
                     /locus_tag="OO566_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6603653.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(98812..99534)
                     /locus_tag="OO566_000231"
     CDS             complement(98812..99534)
                     /locus_tag="OO566_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6603654.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPYQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSC"
     gene            complement(99554..100276)
                     /locus_tag="OO566_000232"
     CDS             complement(99554..100276)
                     /locus_tag="OO566_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6603655.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATFTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(100336..102195)
                     /locus_tag="OO566_000233"
     CDS             complement(100336..102195)
                     /locus_tag="OO566_000233"
                     /inference="COORDINATES: protein motif:HMM:NF011812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCR6603656.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
     gene            complement(102277..102840)
                     /locus_tag="OO566_000234"
     CDS             complement(102277..102840)
                     /locus_tag="OO566_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6603657.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(103524..104009)
                     /gene="sixA"
                     /locus_tag="OO566_000235"
     CDS             complement(103524..104009)
                     /gene="sixA"
                     /locus_tag="OO566_000235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6603658.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(104212..106356)
                     /gene="fadJ"
                     /locus_tag="OO566_000236"
     CDS             complement(104212..106356)
                     /gene="fadJ"
                     /locus_tag="OO566_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6603659.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(106356..107666)
                     /gene="fadI"
                     /locus_tag="OO566_000237"
     CDS             complement(106356..107666)
                     /gene="fadI"
                     /locus_tag="OO566_000237"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6603660.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(107846..108130)
                     /locus_tag="OO566_000238"
     CDS             complement(107846..108130)
                     /locus_tag="OO566_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6603661.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            108502..109842
                     /gene="fadL"
                     /locus_tag="OO566_000239"
     CDS             108502..109842
                     /gene="fadL"
                     /locus_tag="OO566_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6603662.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            110208..111266
                     /locus_tag="OO566_000240"
     CDS             110208..111266
                     /locus_tag="OO566_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603663.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNCQAK
                     YQNEINQLSALGVNFDDI"
BASE COUNT        27857 a        29801 c        27419 g        26251 t
ORIGIN      
        1 acctaaggtg cgcgaaagcc actttttcct tcctgagtta tccacaaagt tatgcacttg
       61 caagagggtc attttcacac tatcttgcag tgaatcccaa acataccccc tatatatagt
      121 gttctaagca gcttccctta ctacaggtag tctgcatgaa actattgcgg aaagaattcc
      181 aaaaacaggt acgacataca tgaatcagaa tctgctggtg acaaagcgcg acggtagcac
      241 agagcgcatc aatctcgaca aaatccatcg cgttctggat tgggcggcag aaggactgca
      301 taacgtttcg atttcccagg tcgagctgcg ctcccacatt cagttttatg acggtatcaa
      361 gacctctgac atccacgaaa ctattatcaa ggctgccgca gacctgatct cccgtgatgc
      421 gccggattat cagtatctcg ccgcgcgcct ggcgatcttc cacctgcgta aaaaagccta
      481 cggccagttt gagccgcctg cgctgtacga ccacgtggtg aaaatggtcg agatgggcaa
      541 atacgataat catctgctgg aagactacac ggaagaagag ttcaagcaga tggacacgtt
      601 tatcgatcac gaccgtgata tgaccttctc ttatgctgcc gttaagcagc tggaaggcaa
      661 atatctggta cagaaccgcg tgaccggcga aatctacgaa agcgcccagt tcctttatat
      721 tcttgttgcc gcgtgcctgt tctcgaacta cccgcgtgaa acgcgcctgc aatacgtgaa
      781 gcgtttttac gacgcggttt ccacatttaa aatttcgctg ccgacgccaa tcatgtccgg
      841 cgtgcgtacc ccgactcgtc agttcagctc ctgcgtactg atcgagtgcg gtgacagcct
      901 ggattccatc aacgccacct ccagcgcgat tgtgaaatat gtttcccagc gtgccgggat
      961 cggcatcaac gccggacgta ttcgtgcgct gggcagcccg attcgcggtg gcgaagcgtt
     1021 ccataccggc tgtatcccgt tctacaaaca tttccagaca gcggtgaaat cctgctctca
     1081 gggcggtgtg cgcggcggtg cggcaacgct gttctacccg atgtggcatc tggaagtgga
     1141 aagcctgctg gtgttgaaaa acaaccgtgg ggttgaaggc aaccgcgtgc gtcatatgga
     1201 ctacggggta caaatcaaca aactgatgta tacccgcctg ctgaaaggtg aagatatcac
     1261 cctgttcagc ccgtccgacg taccggggct gtacgacgcg ttcttcgccg atcaggaaga
     1321 gtttgaacgc ctgtatacca aatatgagaa agacgacagc atccgcaagc agcgtgtgaa
     1381 agccgttgag ctgttctcgc tgatgatgca ggaacgtgcg tctaccggtc gtatctatat
     1441 tcagaacgtt gaccactgta atacccatag tccgtttgat ccgaccatcg cgccagttcg
     1501 ccagtctaac ctgtgcctgg aaattgccct gccgaccaaa ccgctgaacg acgtcaacga
     1561 cgagaacggt gaaatcgcgc tgtgtacgct gtctgctttc aacctgggcg caattaatag
     1621 cctggatgaa ctggaagagc tggcgattct ggcggttcgt gcacttgacg cgctactgga
     1681 ttatcaggat tacccgatcc cggccgccaa acgtggagcg atggggcgtc gtacgctggg
     1741 tattggtgtg attaacttcg cttactatct ggcgaagcac ggtaaacgct actccgacgg
     1801 cagcgccaac aacctgacgc ataaaacctt cgaagcgatt cagtattacc tgctgaaagc
     1861 gtctaatgag ctggctaaag agcaaggcgc gtgcccgtgg tttaacgaaa ccacttacgc
     1921 gaaaggtatc ctgccgatcg atacctataa gaaagatctg gatgccatcg ctaatgagcc
     1981 gctgcattac gactgggaag cattgcgtga atcaatcaaa acgcacggcc tgcgtaactc
     2041 cacgctttct gccctgatgc cgtccgagac ctcttcgcag atctctaacg ccactaacgg
     2101 tattgaaccg ccgcgcggtt acgtcagcat caaagcgtcg aaagacggta ttttgcgcca
     2161 ggtggtgccg gactacgagc acctgcacga cgcctatgag ctgctgtggg aaatgccggg
     2221 taacgatggc tacctgcaac tggtaggtat catgcagaaa tttatcgatc agtcgatctc
     2281 tgctaacacc aactacgatc cgtcacgttt cccgtcagga aaagtgccga tgcagcagtt
     2341 gctgaaagac ctgctcaccg cctacaaatt cggggtcaaa acgctgtatt atcagaacac
     2401 ccgtgacggc gctgaagacg cgcaggacga tctggtaccg tcaatccagg acgatggctg
     2461 cgaaagcggc gcatgtaaga tctgatattg agatgccgga tgcggcgtaa acgccttatc
     2521 cggcctacgg ctcggtttgt aggcctgata agacgcgcca gcgtcgcatc aggctccggg
     2581 tgccggatgc agcgtgaacg ccttatccgg cctacggctc ggatttgtag gcctgataag
     2641 acgcgccagc gtcgcatcag gcacaggatg cggcgtaaaa tgccttatcc ggcattaaac
     2701 tcccaacagg acacactcat ggcatatacc accttttcac agacgaaaaa tgatcagctc
     2761 aaagaaccga tgttctttgg tcagccggtc aacgtggctc gctacgatca gcaaaaatat
     2821 gacatcttcg aaaagctgat cgaaaagcag ctctctttct tctggcgtcc ggaagaagtt
     2881 gacgtctccc gcgaccgtat agattaccag gcgctgccgg agcacgaaaa acacatcttt
     2941 atcagcaacc tgaaatatca gacgctgctg gattccattc agggtcgtag cccgaacgtg
     3001 gcgctattgc cgcttatttc tattccggaa ctggaaacct gggtcgaaac ctgggcgttc
     3061 tcagaaacga ttcattcccg ttcctatact catatcattc gtaatatcgt taacgatccg
     3121 tctgttgtgt ttgacgatat cgtcaccaac gagcagatcc agaaacgtgc ggaagggatc
     3181 tccagctatt acgatgaact gatcgaaatg accagctact ggcaccttct gggcgaaggc
     3241 acccacaccg ttaacggtaa aaccgtaacc gttagcctgc gcgagctgaa gaaaaaactg
     3301 tatctctgcc tgatgagcgt taacgcgctg gaagcgatcc gcttctacgt cagctttgct
     3361 tgttcttttg cctttgcaga acgcgaactg atggaaggta acgccaagat tattcgcctg
     3421 attgcccgcg atgaagccct gcacctgacc ggtactcagc atatgctgaa tctgctgcgc
     3481 agcggcgcgg atgatccgga gatggcggaa attgccgaag aatgtaagca ggagtgctat
     3541 gacctgtttg tccaggcggc acagcaagag aaagactggg cggattatct gttccgtgac
     3601 ggttcgatga ttggtctgaa taaagatatt ctctgccagt acgttgaata catcaccaat
     3661 atccgcatgc aggcagtcgg tttggatctg ccgttccaga cgcgctccaa cccgatcccg
     3721 tggatcaaca cctggctggt gtctgataac gtgcaggttg ctccgcagga agtggaagtc
     3781 agttcttatc tggtcgggca gattgacgcg gaagtggaca ccgacgattt gagtaacttc
     3841 cagctctgat ggcccgcgtt accctgcgca tcactggcac acaactgctg tgccaggatg
     3901 aacacccttc ccttctggcg gcgctggaat cccacaatgt ggcggttgag taccagtgcc
     3961 gcgaaggtta ctgcggctcc tgtcgcaccc gactggtcgc aggtcaggtg gactggattg
     4021 ccgaaccgtt agcctttatt cagccggggg aaattttgcc ctgttgttgc cgggcaaaag
     4081 gcgatattga aatcgagttg tgatcgggta ctagcgccag atacgacgct tacgcgtatt
     4141 atctggccta caacgattac atggcgtagt aatacgcttt cacctgctcc cagtccgctt
     4201 tagggatcgg ctccagatat ttttccagct ggcggaagtc atgattaatc gctttatcgc
     4261 gacgcaagcg gcgacgactt ttctccagat caagaaaacc agcttctgcc ttaccttctg
     4321 ttttcacata gatatggcga acatagcaac agccatgctg acgattaacg ctatgcattt
     4381 tcttaaacgc cagtgccact gctttcaaca tggcttgccg tacttcgtca gaataaggcg
     4441 atactgcatg ccgggcatac cagtcagcaa tgctgatgaa ccccgccata tcttcagtca
     4501 ccagcaacgc gcgccattca ccctcaattt tcaccgcttc accaaaaacg atcttcggca
     4561 cgatgacacc ggcccgttcc agttctttaa ttaccgcaac ctcacggaca atcgttggtc
     4621 ggccgaacgg ataacgtact gaatgaaaca gatgatgcgt catgcgcttt acatacagct
     4681 ttttgccgtt gcgctcgacg cattgcaccc tgctcatacc attacggcga tagttaggct
     4741 cttcaaccca gtcgccctct gttgcccacc agtgattaaa ttcgtcgtac tttgctgaaa
     4801 ctgccatacc ctatcgcctg tcatttttat taacgacaat gactataggt ggttacctga
     4861 gcaaaatctt aatgaaacgt gtcgtattcc gctacaggtg tgacaccgct tttgcccttc
     4921 atttgcagcg catagattcc accagccact accactaata gtgcgcccag cagataaaat
     4981 gtgaatttaa tcagtcggat acagcgatga attttattcg acagggatta ggcatcgcct
     5041 tacaaccaga gttaacgctg aaaagcattg caggtgaatt gtgttccgtt cctcacgaac
     5101 caaccttcta tcgacagatt tcgttgctga ctaaagaaaa gccggtagaa ggcagtccac
     5161 tgtttttact acaaatgtgc atggaacaat tagtggcgat tggaaaaatt tgatatgaga
     5221 caggatggcg catgaacgcc atcctgcagt aacattactc tttattaaga aattttactg
     5281 ccttatcagg gaaatcagta aacagcccat ttacacccgc tttgttatac agagcatcat
     5341 ataactgatt cacatcagga gtgtattcag gcagtttatc tgaccgcacg gtataaggat
     5401 gcactaccag tttattctgc tgagcatctt gcaccatgcc agtgagtttg atattacccg
     5461 gctgcgatgt ctcctcaatc aacatatggt aatccggacc aataccatct gcatattccg
     5521 ccacctgttt catggcaccc ggcttaaaca tccagtcgta gttgtaatta acccagcttc
     5581 catccggctg tttctgctgc gtttcattcc agtcggtata ggcaatcagc tgtaccagat
     5641 tgagctccat gcccattttg ggttccagct cattcttaat acgcttcagc tcatcagcat
     5701 caaaacattg caaataaact ttatcgtctt taccggtgta accatatttc ttcagcactt
     5761 ccagcgtttt tgccgcaata tccttccctt cctgatgatg gaaccacggc gctttgattt
     5821 ctggatagat accgatattt ttcccggtag agtgatttaa cccctgaaca aattcaatct
     5881 cttcttcaaa ggtgtgcacc cggaagtcgg acttacccat tgggaaacgc cccggataag
     5941 tctgtacttt tttaccgttt tcaatatcga aaccttcggt aaatttcagc gacttaattt
     6001 catccagcgt gaaatctatc gcgtagtaac gaccgtcttt gcgcgcccga tccgggaaac
     6061 gatcggcaac atcagtaaca cgatcgagat aatggtcatg cagaacaacc agatggtcgt
     6121 ctttggtcat caccaaatcc tgctccagat aatccgctcc ctgcgcatac gccatcgctt
     6181 ttgctggcag cgtatgctcc ggcaaatatc cactggcacc gcgatgggcg atgactattt
     6241 tttcgttgct gtccgccgcc attgcactgc ttcccatgac tatagtgctc atcatgatcg
     6301 ccatgctaag gtttttcagc ttcaatttca tgccattagc ctccgttgcg tttttgcagt
     6361 aattgttcat ggcgacgttt ttcgccaatc atcacaacaa tcaacaagat aaccgccaga
     6421 atgctgccgc caatcattac cataaagccg ccatcccagc cgaagaagtc cacggtgtag
     6481 ccaacaatcg cgctcgccgc caccgaaccg cccaggtaac caaacagccc ggtaaagccc
     6541 gctgccgtac ctgccgcttt tttcggtgcc agttccagcg catgcagacc gatcagcatc
     6601 acaggaccgt agatcaggaa gccgataaca atcatacaaa tcatatcgac ggttgggtta
     6661 cctgccgggt tcatccagta aacgatagtc gcgatggtca ccagtgtcat aaagaagacg
     6721 ccggttgccc cacggttacc acggaagact ttgtccgaca tccagccgca cagcagggtg
     6781 cccggaatac ccgcatactc atagaagaag taggcccagg aggatttatc cagcgcgaaa
     6841 tgcttaacct ctttcagata agtcggtgac cagtcgagga tgccgtaacg cagcagataa
     6901 acgaacacgt tggcgatggc gatgtaccac agcagtttgt tcggcagcac gtactgcatg
     6961 aagatttgct tcgcggtcag ctcctgttcc gctttttcgt tatagtcgtc cggataatca
     7021 tttttgtact cttcgatcgg cggcaagcca caggattgcg gggtatcgcg catcatcgca
     7081 aaggcgaata atgccaccag aatggcgcag aaagcaggca tatagagcgc cgcatgccag
     7141 tcattgaacc aggccatccc cagcaggaac agcagcggcg gaataccacc accgacgttg
     7201 tgcgcacagt tccacactga cacaatgccg ccacgttctt tctgcgacca ccagtgcacc
     7261 atagtacgac cacacggcgg ccaccccatc ccctggaacc aaccgcagag gaacaacagt
     7321 acaaacatca ccgcaatgct cgacgtcgcc catggcacaa agcccataaa caacatcact
     7381 gccgccgcca gaatcaaacc tgcgggcagg aaaacgcgcg gattcgagcg atccgatacc
     7441 gaacccatga tgaatttcga aaatccataa gcaatcgaga tccccgaaag ggcaaaacct
     7501 aaatcaccgc gtgagaatcc ctgctcaacc agataaggca tagcaagcgc aaagttctta
     7561 cgaaccaaat agtaagccgc atagccaaag aatatcccca ggaaaatttg ccagcgcaat
     7621 cgacgataag tcggatcgat ctccgcggca ggtaagcgcg ctttgtgtgg cgctggttta
     7681 aaaatactca acattgatag cctccgtggc ccgtggtctt atttatgatt aacagcctga
     7741 ttcagtgaga gaacctgccg tttcttgagt tgccgcgatg ttaagaaaac attcataaat
     7801 taaatgtgaa ttgccgcaca cattattaaa taagatttac aaaatgttca aaatgacgca
     7861 tgaaatcacg tttcactttc gaattatgat cgaatatgcg cgaaatcaaa caattcatgt
     7921 ttttactatg gctaaatggt aaaaaacgaa cttcagaggg ataacaatga aaactcgcga
     7981 ctcgcaatca agtgacgtga ttatcattgg cggcggcgca acgggagccg ggattgcccg
     8041 cgactgtgcc ctgcgcgggc tgcgcgtgat tttggttgag cgccacgaca tcgcaaccgg
     8101 tgccaccggg cgtaaccacg gcctgctgca cagcggtgcg cgctatgcag taaccgatgc
     8161 ggaatcggcc cgcgaatgca ttagtgaaaa ccagatcctg aaacgcattg cacatcactg
     8221 cgttgaacca accaacggcc tgtttatcac cctgccggaa gatgacctct cattccaggc
     8281 cacttttatt cgcgcctgcg aagaagcagg gatcagcgca gaagctatag acccgcagca
     8341 agcgcgcatt atcgaacctg ccgttaaccc ggcactgatt ggcgcggtga aagttccgga
     8401 tggcaccgtt gatccatttc gtctgaccgc agcaaacatg ctggatgcca aagaacacgg
     8461 tgccgttatc cttaccgctc atgaagtcac ggggctgatt cgtgaaggcg cgacggtgtg
     8521 cggtgttcgt gtacgtaacc atctcaccgg cgaaactcag gcccttcatg cacctgtcgt
     8581 ggttaatgcc gctgggatct gggggcaaca cattgccgaa tatgccgatc tgcgcattcg
     8641 catgttcccg gcgaaaggat cgctgctgat catggatcac cgcattaacc agcatgtgat
     8701 caaccgctgc cgtaaacctt ccgacgccga tattctggtg cctggcgata ccatttcgct
     8761 gattggtacc acctctttac gtattgatta caacgagatt gacgataatc gagtgacggc
     8821 agaagaggtt gatattctgc tgcgtgaagg ggaaaaactg gcccccgtga tggcgaaaac
     8881 gcgcattttg cgggcctatt ctggcgtgcg cccgctggtt gccagcgatg acgacccgag
     8941 cggacgtaac gtcagccgtg gcatcgtgtt gctcgaccat gctgaacgcg atggtctgga
     9001 cggatttatc accatcaccg gtggcaaact gatgacctat cggctgatgg ctgaatgggc
     9061 taccgacgcg gtatgccgca aactgggcaa cacgcgcccc tgtacgactg ccgatctggc
     9121 actgcctggt tcacaagaac ccgctgaagt taccttgcgt aaagtcatct ccctgcctgc
     9181 cccgctgcgc ggttctgcgg tttatcgtca tggcgatcgc acgcctgcct ggctgagcga
     9241 aggccgtcta caccgtagcc tggtatgtga gtgcgaagcg gtaactgcgg gtgaagtgca
     9301 gtacgcggta gaaaatttaa acgttaatag cctgctggat ttacgccgtc gtacccgtgt
     9361 ggggatgggc acctgccagg gcgaactctg cgcctgccgc gctgccggac tgctgcaacg
     9421 gtttaacgtc acgacgtccg cgcaatctat cgagcaactt tccaccttcc ttaacgaacg
     9481 ctggaaaggc gtgcaaccca tcgcctgggg agacgcactg cgcgaaagcg aatttacccg
     9541 ctgggtttat cagggactgt gtggtctgga gaaggagcag aaagatgcgc tttgatactg
     9601 tcattatggg cggcggcctc gccggattac tctgtggcct gcaactgcaa aaacacggcc
     9661 tgcgctgtgc cattgtcacg cgtggtcaaa gcgcgttgca tttctcatcc ggttcgctgg
     9721 atttgctgag ccatctgcca gatggtcaac ccgtggcaga cattcacagt ggactggaat
     9781 ctttgcgtca gcaggcacct gcacatcctt actctcttct cgggccacaa cgcgtgctcg
     9841 atctcgcttg ccaggcgcag gcattaatcg ctgaaagcgg tgcgcaattg cagggcagcg
     9901 tagaactggc tcaccagcgg attacgccgc tcggcacttt gcgtgcaacc tggttaagtt
     9961 cgccagaagt tcccgtctgg ccgctacccg cgaagaagat atgtgtagtg gggataagcg
    10021 ggctgatgga tttccaggcg catcttgcgg cggcttcgtt gcgcgaactg gatctttcgg
    10081 ttgaaaccgc agaaatagag ctgccggaac tggatgtcct gcgcaataac gccaccgaat
    10141 ttcgcgcggt gaatatcgcc cgtttccttg ataatgaaga aaactggccg ctgttacttg
    10201 atgcgcttat tcctgtcgcc aatacctgcg aaatgatcct gatgcccgcc tgcttcggcc
    10261 tagccgatga caaactgtgg cgttggttga atgaaaaact accttgttca ctgatgcttt
    10321 tgccaacgct gccgccttcc gtgctgggca ttcgtctgca aaaccagtta cagcgccagt
    10381 ttgtgcgcca gggtggagtg tggatgccgg gcgatgaagt gaaaaaagtg acctgtaaaa
    10441 atggcgtagt gaatgaaatc tggacccgca atcacgccga tattccgcta cgtccacgtt
    10501 tcgcggttct cgccagcggc agtttcttta gtggcggact ggtagcggaa cgtaacggca
    10561 ttcgcgagcc gattctcggc cttgatgtgc tacaaaccgc cacgcggggt gaatggtata
    10621 agggcgattt ttttgcgccg caaccgtggc agcagttcgg tgtaaccact gatgagacgc
    10681 tacgcccgtc gcaggcaggg caaaccattg agaacctgtt tgccatcggt tcggtgctgg
    10741 gcggatttga tcccatcgcc cagggatgcg gcggcggtgt ttgtgccgtc agtgctttac
    10801 atgccgctca acagattgcc caacgcgcag gaggccaaca atgaacgaca ccagcttcga
    10861 aaactgcatt aagtgcaccg tctgcaccac cgcctgcccg gtgagccggg tgaatcccgg
    10921 ttatccaggg ccaaaacaag ccgggccgga tggcgagcgt ctgcgtttga aagatggcgc
    10981 actgtatgac gaggcgctga aatattgcat caactgcaaa cgttgtgaag tcgcctgccc
    11041 gtccgatgtg aagattggcg atattatcca gcgcgcgcgg gcgaaatatg acaccacgcg
    11101 cccgtcgctg cgtaattttg tgttgagtca taccgacctg atgggtagcg tttccacgcc
    11161 gttcgcacca atcgtcaaca ccgctacctc gctgaaaccg gtgcggcagc tgcttgatgc
    11221 ggcgttaaaa atcgatcatc gtcgcacgct accgaaatac tccttcggca cgttccgtcg
    11281 ctggtatcgc agcatagcgg ctcagcaagc acaatataaa gaccaggtcg ctttctttca
    11341 cggctgcttc gttaactaca accatccgca gttaggtaaa gatttaatta aagtgctcaa
    11401 cgcaatgggt accggtgtac aactgctcag caaagaaaaa tgctgcggcg taccgctaat
    11461 cgccaacggc tttaccgata aagcacgcaa acaggcaatt acgaatgtag agtcgatccg
    11521 cgaagctgtg ggagtaaaag gcattccggt gattgccacc tcctcaacct gtacatttgc
    11581 cctgcgcgac gaatacccgg aagtgctgaa tgtcgacaac aaaggcttgc gcgatcatat
    11641 cgaactggca acccgctggc tgtggcgcaa gctggacgaa ggcaaaacgt taccgctgaa
    11701 accgctgccg ctgaaagtgg tttatcacac tccgtgccat atggaaaaaa tgggctggac
    11761 gctctacacc ctggagctgt tgcgtaacat cccggggctt gagttaacgg tgctggattc
    11821 ccagtgctgc ggtattgcgg gtacttacgg ttttaaaaaa gagaactacc ccacctcaca
    11881 agccatcggc gcaccactgt tccgccagat agaagaaagc ggcgcagatc tggtggtcac
    11941 cgactgcgaa acctgtaaat ggcagattga gatgtccaca agtcttcgct gcgaacatcc
    12001 gattacgcta ctggcccagg cgctggctta aactcccttc tgatgcccgg taagcatgtg
    12061 gttaccgggc atttttgcgt acacgattcc gtgcccaatg tatgcgttgc aacgcagtga
    12121 aaattcctct gaaaacgtct cgcaaaggct gaaactggca gatgtcaaag gcctgggata
    12181 accgtaatgt cgcgtcatca taaatatcag gtgacggaca accatgaccg aatcaacaac
    12241 ctcctccccg catgatgcgg tatttaaaac ctttatattc acacccgaaa ccgcacggga
    12301 ttttctcgaa atacatttac cagaaccact gcgcaagctt tgcaacctgc aaaccttacg
    12361 cctggaaccc actagtttta ttgaaaaaag tttacgcgct tactactcgg atgttttgtg
    12421 gtccgtggaa accagcgacg gtgacggcta tatctactgc gtgattgaac atcaaagctc
    12481 tgcagaaaag aatatggctt ttcggccaat gcgctatgcc actgccgcca tgcagcgtca
    12541 cctagataaa ggctatgaca gagttccgct ggtggtgcca ttgctgtttt atcatggcga
    12601 aacatcgccc tacccgtact cacttaactg gctggatgag tctgacgatc cgcaacttgc
    12661 ccggcagttg tacaccgaag cttttccgtt ggtggatatc accatcgtac ctgacgatga
    12721 gatcatgcaa catcggcgta tagctctgct ggaactgatt caaaagcata ttcgcgacca
    12781 cgatttaatc ggcatggtcg acaggatcac cacgcttttg gttagaggct tcactaatga
    12841 cagccagcta caaacactgt ttaattatct gctgcaatgc ggcgatacct cccgtttcac
    12901 ccgttttatt caggagattg ccgaacgttc accactacaa aaggagagat taatgactat
    12961 tgctgaacgg ctacggcagg aagggcatca aattggctgg caggaaggta tgcatgaaca
    13021 agccattaaa attgccttgc gcatgctgga gcagggcttt gaacgtgaga ttgtgctggc
    13081 ggcaacccaa ctcactgatg ctgatattcc gaactgttat taaaaggaga gggagagatt
    13141 gatgactatt gctgaacggc tacggcagga aggacatcaa attggctggc aagaaggtaa
    13201 attagtaggt ttgcagcaag gtaaattaga aggtttgcag gaaggtatgc atgaacaagc
    13261 cattaaaatt gctttgcgca tgctggaaca gggcattgat cgtgaccaag tgctcgcggc
    13321 cacccagcta agcgaagccg atctggcagc gaataaccac taattaacac aggcccacag
    13381 ccgatcccca tgggcctttg atatcaataa ctacctttta tgcgtggccc atttttgcct
    13441 gatttaaaca tcgccagtcg ttgatccagg gcatcgctgt agagcatcgt gtcaacgcca
    13501 acagcgacaa agttcgctcc ccacgccagg cattgctgcg ccatatcagg agccacagcc
    13561 agaaaaccag ccgctttacc cgcagcacgg atccgccgaa tgctggtttc aataattcgc
    13621 tgcacttccg ggtgcccggc gttatccggg tagcccaacg acgcagaaag atccgctggt
    13681 ccaataaaca cgccatcaat cccttcgacg tcgaggatct cgtccaggtt atccagtgcc
    13741 gttttacttt ccacctgcac taacagacaa agcgaatcgt tgacttgcgc catgtaattc
    13801 tcaatgcgtc cccagcgcgc agcccgtgcc acactggccc caacaccacg ctcaccgtag
    13861 ggagggttgg acaaattttg ccggaggggg aatgtgccgg tgcagtaaac cggcacagag
    13921 tggcgaaatc agacggataa agtcatggac tcaacaacct caatccagcc atgctcacta
    13981 gcgatgtcct ggccgtttaa ccaacggcgt agcatattca gtgccatcat tgcgcacact
    14041 tcctgacgga tagccaggct gtagcgtgta gtgctgaaac gcacacgcag agcgaaagtg
    14101 ccgtctggcg tggctagcgc aaagttgaga tgctcgttct cgaaacccga aacagccagt
    14161 gccagcccgg caaaatggtt ggcccgccgt tctgtaatcc agtgcgcagt ttgcgccagg
    14221 gtttcttcct gtgaaggaat cacttcacac gccagcaatg gagcacctgc gcgagaaagt
    14281 tgcaaagcca ataaaccacc ggtgaattgc tcgctcaacg tcaggctgaa ctggcggttt
    14341 tgcaattcgc gactgatctg cgcgggcagt ccttcggtgc cttcgaaaat cacgctctgt
    14401 ccggcaacac gtttaacgtc cagccacagt ttttccatcg cctgttgctc gcttgccggt
    14461 ccggtgagtt tcagttcgat gatcggcatt gaggagcgat agcccattgt tacgcccggc
    14521 ggcagttgta gagtgtccag gctttgtgcc agatcgcttt ccgaacgacc aaaggtagtt
    14581 aaacgcagac aaaccggcgg ctgtggtaaa gaaaaacgct cgcgcaggcg cggcaggatt
    14641 tcgtgctcga ccatcacctt aaattctgac ggtacgccgg gagtaaagaa catcaggcaa
    14701 cgattaagct gcacggcaaa accacaggcg gtgcctaccg ggttattgat aaattcagca
    14761 ctggcaggca gctccgcttg tttacggctg ctcggtgcca ttactcgtcc acgttcgtgg
    14821 aaatagcgtt ccatctcttt gagccaggct tcatgcagca caaggccttc accttttgct
    14881 gtcgcagcgg cgagtgcgct taaatcatcg ctggtcggtc ccagcccgcc gttaacgatc
    14941 agcacatcgg cgtgctgact acgttcgcga agaatggtga ctaagtcatc aaggttatcc
    15001 cccaccgtat tgcggcgaga taatggcagc ccctgatgaa agaaaaaatc ggccagccag
    15061 gcagcgttag tgtcaacgat ttgcccgtat aacacttcat ccccggtgga tagcatttcc
    15121 acttttaaca tggttatctc ctgcttttgt tatcgaatca ctataacgca aagggagata
    15181 aggcgtagta agaaactgaa tgccgcgccg gaagggatca gaaactggcg ttaaccccaa
    15241 catacaggcc gtcagccaca gcgttgtcgc ggttaccgtc tttacccgac agattcaggt
    15301 agcgataacc cgcctcaata ctgactggac gcataatggt gtaacgcgcg ccagcattcg
    15361 cttcctcata actttgaata ccgctggaga gcgaatccgg agagtagtaa tactcgccaa
    15421 acaaacggaa gctgttgcca attttccact gcaaaccacc tcctaccgct gcggcataac
    15481 cttcatcgcc gtaattcggg ttggtgtaca cgccttttcc gccaacggtc gccattaacg
    15541 gcccgagagg cagattcaac cccagcccca cgcccgcgac gtcaccgtcg tcgtcgttat
    15601 gtgtccagtt accgcttaaa gccaggcccg tcgattcagt cccaaaaccg acaccaatat
    15661 tggtgtattc tttacccgcc tggccgctga tgctcattgc attgaccgat gcagaaacca
    15721 acagcattcc ggccagacct gctagcgcaa tttttttcat tatccccttt cccacaagca
    15781 attaatcaaa attcccaaag ctgcgggatt gtactgcctg gcacagagga ttcaatgagc
    15841 gaaaaaaggg atctagtcac ttattgtaac taaatacttc ttaaataatt aaacgccgaa
    15901 aaaataaaca cactttaccg atacacacaa aaataataat aagtgcagtt atcattgcaa
    15961 aagagattac tgtcactttc acatatttct gagttaccgt attctcatgg tttcttttta
    16021 tttataagga atacccctgt gcgacaacgg actattgtat gccctttgat tcaaaatgat
    16081 ggtgcttatt tgctgtgcaa aatggccgac gatcgcggcg ttttccccgg tcaatgggcg
    16141 ctttcgggtg gcggcgtgga gtctggcgaa cgaattgaag aggcactacg ccgcgaaatt
    16201 cgcgaagaac tgggagaaca gctgcttttg acagaaatca cgccgtggac cttcagcgat
    16261 gatattcgca ccaagacata tgcagatggt cgcaaggaag agatttatat gatttacctg
    16321 atttttgact gcgtttctgc caaccgagaa gtgaaaataa acgaagagtt tcaggactac
    16381 gcgtgggtaa aacctgaaga tctggtgcat tatgatttaa atgttgccac ccgaaaaacg
    16441 ttacgtttga aaggtcttct ttaacaatga tgacagccat cactggctgt caggttgaaa
    16501 atttagtggt taacgaattc cccatccaga taaactttgc ctttttccac atgcatgact
    16561 aaaccatcca gataatcagg tttaaatatc gcatcacgct tatttttagc aatatatgtc
    16621 aggcaatgat tatgggtgaa aataaccata tttttatcag gcgctttgct ttgtaagtcc
    16681 ttaattgcac tataaatctc attaccgcac tgaagaagtc gtttatctac cgtcagcttt
    16741 ttacccgcgg aaaaccaggt cgccgactga atggtccgga cggtattact ggaataaaga
    16801 tcgaaatcag ggatgtcagc gctaaaagcg ttgcccagtt cacgggcatc ctgggtacct
    16861 ttaaccgtaa tacctgtttt atctgacaag cattgattgg ttgaacgatc gcaacgttca
    16921 gcatggcgaa acaaaacgac aaccgggtgc tgctgtgcca gtctggccaa tgttttattg
    16981 tcgatacgtg gcaaaccatt gctactccag gcggcatgag tacccagtcc ggcaattgca
    17041 gcgagcgcca gtaaaatgat gaaatatttt tttgacttca atgatgagcg gcaaaaagct
    17101 aacacgtaaa ctccacctgt agacaagcgc aaccagacaa ttaccgtgaa attgagcgac
    17161 atttctggcg ataattagca gttggtgtaa tattaaaaat cctacgatgt cggcaaaatg
    17221 cctcaaattt ttgccaaatg caaagcctaa ataagaaaaa atataaaaat ttcaatattt
    17281 acgtctaata ttagtttctt aaggttaagt taatattcta tccttaaaat ttcgctccaa
    17341 atggcaaaat atacacaaca ctctttatag caaatataag tggacaggta ttcaatggcg
    17401 gaaggaaaag caatgtcaga atttttgcct ttttcgcgac cagcaatggg cgtggaggaa
    17461 ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca caaccggtcc gaagaatcag
    17521 gcgcttgagc aagccttttg ccagttgacg ggaaatcagc atgccatcgc ggtcagttca
    17581 gccaccgccg gaatgcatat cacgctaatg gcgttggaaa ttggcaaggg cgatgaagtg
    17641 attacgcctt ccctgacctg ggtttcaacc ctcaatatga tttccttgct gggtgcaacg
    17701 ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca cgcctgaagc gatcgaatca
    17761 gccattacgc cacgcactaa agccatcatt ccggtgcatt atgccggtgc gccagcagat
    17821 attgacacca ttcgcgccat tggcgaacgt tacggcatcg cagttatcga agatgctgcc
    17881 catgccgtcg gtacgtatta caaagggcga catattggcg caaaaggtac cgctattttt
    17941 tcatttcatg ccattaaaaa tattacctgt gctgaaggtg gcctgattgt aactgataat
    18001 gaaaaccttg cccgccagct acggatgctg aaatttcacg gtctgggtgt cgatgcctat
    18061 gacagacaaa cctggggccg tgcaccgcag gctgaagtct taacaccggg ctataagtac
    18121 aatctgaccg atattaacgc cgcgattgcc ctgacacagt tagtcaaatt agagcacctc
    18181 aacacccgtc ggcgcgaaat tgcccagcaa tatcagcaag cactggcagc tctccccttt
    18241 cagccattaa gccttcccgc ctggccgcac gttcacgcct ggcatctgtt tattattcgt
    18301 gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga tggaagcgtt aaaagaaaga
    18361 ggcattggta ccgggttaca tttccgcgcc gctcacacac aaaaatatta tcgcgagcgt
    18421 tttcccacgc tgtcgttacc gaataccgaa tggaatagcg aacgcatctg ttcgttgccg
    18481 ctgttcccgg atatgactac cgccgatgcc gaccgcgtca tcacagccct tcagcaactc
    18541 gcaggacaat aagccatgtt tgaaatccac cctgttaaga aagtctcggt ggttattccc
    18601 gtttataacg agcaggaaag cttaccggaa ttaatcaggc gcaccaccac agcctgtgaa
    18661 tcgttgggga aagagtatga gatcctgctg attgatgacg gcagtagcga taattccgcg
    18721 catatgctgg tcgaagcctc acaagcggag aacagccata ttgtgtctat tttgcttaac
    18781 cgcaattacg ggcaacattc agcgattatg gcgggattca gtcacgttac tggcgactta
    18841 attattaccc ttgatgccga tctccagaat ccgccagaag aaatcccccg cctggtggca
    18901 aaagccgatg aaggttacga cgtggtaggg actgtacgcc agaaccgcca ggacagctgg
    18961 tttcgtaaaa cagcttcgaa gatgattaac cggcttattc agcgcaccac tggcaaagcg
    19021 atgggtgact acggttgtat gctgcgcgcc tatcgccgtc atattgtcga tgcgatgttg
    19081 cactgccatg aacgcagcac ctttatcccg attctggcga atatcttcgc ccgccgtgca
    19141 attgaaattc cagtacatca tgccgagcgt gagtttggtg aatccaaata cagttttatg
    19201 cgcctgatta atttgatgta cgacctggtg acctgcctta ccacaacgcc gctacgtatg
    19261 ctgagtctgc tcggcagcat tattgcgatt ggaggtttta gcattgcggt gctgctggtg
    19321 attttacgcc tgaccttcgg accacaatgg gcggcagaag gcgtctttat gctatttgcc
    19381 gtgctgttta cttttattgg cgctcagttt atcggcatgg gattactcgg tgaatatatc
    19441 ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt ttgttcagca agttatccgt
    19501 ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt tgcctaccac gatatgggat
    19561 gcctcggtat tgaagctctg ctggctgccg gttacgaaat tagcgccatt tttacccata
    19621 ccgataatcc cggtgaaaaa gccttttatg gttcggtggc tcatctggcg gcggaaagag
    19681 acattccggt ttatgcgccg gataacgtta atcatccgct gtgggtggaa cgcattgccc
    19741 aactgtcacc agaggtgatt ttctcttttt attatcgcca tcttatttgc gacgagattt
    19801 ttcagctcgc tcccgcaggt gcatttaatc tgcacggttc gctgttacca aaatatcgtg
    19861 gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac ggaaacgggc gttacattgc
    19921 accgaatggt aaaacgtgcc gatgctgggg ccattgtggc gcaactgcgc gttgccattg
    19981 cgccagacga tatcgctatt acgctgcatc ataaattgtg ccatgccgcg cgccagctac
    20041 tggaacagac attacccgcc attaaacacg gtaatattct ggaaatcgcc cagcgcgaaa
    20101 acgaagccac ctgttttggt cgcagaacgc cggatgacag tttccttgaa tggcataaac
    20161 cggcatccgt actgcacaac atggtacgtg ccgttgccga tccgtggccg ggtgccttca
    20221 gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg tgttcatcct catgccagca
    20281 aagcacagcc ggggagcgtg atttctgttg cgccactgct gattgcctgt ggcgatggcg
    20341 cgctggaaat cgtcaccgga caggcgggcg acggcattac tatgcagggc tcgcaattag
    20401 cgcagatgct gggcctggtg caaggttcac gcttgaatag ccagcctgcc tgcaccgccc
    20461 gacgccgtac ccgggtactc atcctcgggg tgaatggctt tattggcaac catctgacag
    20521 aacgcctgct gcgcgaagat cattatgaag tttacggtct ggatattggc agcgatgcga
    20581 taagccgttt tctgaatcat ccacattttc actttgtcga aggcgatatc agtattcatt
    20641 ccgaatggat tgagtatcac gtcaaaaaat gtgatttcgt cttgccgctg gtggcgatag
    20701 ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt tgaactcgat tttgaagaga
    20761 atctgcgcat tatccgctac tgcgttaagt accgtaagcg aatcatcttc ccgtcaactt
    20821 cagaagttta tgggatgtgt agcgataaat acttcgatga ggaccattct aatttaatcg
    20881 tcggcccggt gaataaacca cgctggattt attcggtgtc aaaacaatta cttgatcggg
    20941 tgatctgggc ctatggcgaa aaagagggtt tacagttcac cctcttccgc ccgtttaact
    21001 ggatgggacc acgactggat aaccttaatg cagcgcgaat tggcagctcc cgcgctatta
    21061 cgcaactcat tctcaatctg gtagaaggtt caccaattaa gctaattgat ggcggaaaac
    21121 aaaaacgctg ctttactgat atccgggacg gtatcgaggc gttgtaccgc attatcgaaa
    21181 atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg caatcctgag aacgaagcga
    21241 gcattgagga actgggcgag atgctgctgg cgagcttcga aaaacatccg ctgcgccatc
    21301 atttcccacc gtttgcgggc tttcgcgttg tcgaaagtag cagctactac ggcaaaggat
    21361 atcaggacgt agagcatcgt aaaccgagca tccgcaatgc ccaccgctgc ctggactggg
    21421 agccgaaaat tgatatgcag gaaaccatcg acgaaacgct ggatttcttc ctgcgcaccg
    21481 ttgatcttac ggataaacca tcatgaccaa agtaggctta cgcattgatg tcgatacctt
    21541 tcgtggcacc cgtgaaggcg tgccgcgtct gctggaaatc ttgagtaagc ataatattca
    21601 ggccagcatt tttttcagcg tcggcccgga caatatgggc cgccatctct ggcgactggt
    21661 gaagccacag tttttgtgga agatgctgcg ctcaaacgcg gcatcgcttt atggctggga
    21721 tattttactg gcaggtacgg cctggccagg taaagagatt ggtcatgcca atgccgatat
    21781 cattcgtgaa gcggctaaac atcacgaagt cggtctgcac gcctgggatc accatgcctg
    21841 gcaagcccgt agcggtaact gggatcggca aacaatgatc gacgatattg cacgcggtct
    21901 tcgcactctg gaagagatta tcggtcaacc ggtaacctgt tctgccgctg cgggctggcg
    21961 tgccgaccag aaggtgatcg aagcaaaaga agcgttccat ttgcgctaca acagcgattg
    22021 tcgtggggcc atgccgttcc gtccactgct cgaatcagga aaccctggca ctgcgcaaat
    22081 tccggtgacc ttacccacct gggatgaagt gattggtcgg gatgtgaaag cagaagattt
    22141 taacggttgg ttactcaacc gcatcctgcg agataaaggc acgccggttt ataccattca
    22201 tgcagaagtt gaaggctgcg cttatcagca taattttgtg gatctcctca aacgcgcagc
    22261 tcaggaaggc gtgacatttt gccctttaag cgaactgtta tcagagacgt tgccgctcgg
    22321 acaagttgtt cgcggaaata ttgccggacg tgaaggctgg ctgggttgcc aacaaattgc
    22381 gggtagtcgc tgatgaaatc ggtacgttac cttatcggca tcttcgcgtt tattgcctgc
    22441 tattacctgt taccgatcag cacgcgtctg ctctggcaac ccgatgaaac gcgttatgcg
    22501 gaaatcagtc gagaaatgct ggcatccggc gactggattg tgccccatct gttagggcta
    22561 cgttatttcg aaaaacccat tgccggatac tggattaaca gcattgggca atggctattt
    22621 ggcgcgaata actttggtgt gcgggcaggc gttatctttg cgaccctgtt aactgccgcg
    22681 ctggtgacct ggtttactct gcgcttatgg cgcgataaac gtctggctct actcgccaca
    22741 gtaatttatc tctcattgtt tattgtctat gccatcggca cttatgccgt gctcgatccg
    22801 tttattgcat tctggctggt ggcgggaatg tgcagcttct ggctggcaat gcaggcacag
    22861 acgtggaaag gcaaaagcgc aggattttta ctgctgggaa tcacctgcgg catgggggtg
    22921 atgaccaaag gttttctcgc ccttgccgtg ccggtattaa gcgtgctgcc atgggtagcg
    22981 acgcaaaaac gctggaaaga tctctttatt tacggctggc tggcggttat cagttgcgta
    23041 ctgacggttc tcccctgggg actggcgata gcgcagcggg agcctgactt gtggcactat
    23101 tttttctggg ttgagcatat tcaacgcttt gcactagatg atgcccaaca tagagctccg
    23161 ttctggtact acgtgccggt catcattgcc ggtagcctgc cgtggctggg attactcccc
    23221 ggtgcactgt acacaggctg gaaaaaccgc aagcattccg caaccgtcta tttgttgagc
    23281 tggacgataa tgccgctgct gtttttctcc gtcgctaaag gcaaattgcc cacctatatt
    23341 ctttcttgct ttgcacctct ggcaatgctg ttggcgcatt acgctttgct ggcagcaaaa
    23401 aataatcctc tggcgctgcg gattaatggc tggattaaca tcgcttttgg cgtcactggc
    23461 attattgcca catttgtgat ctccccctgg ggaccaatga acacgccggt gtggcaaacc
    23521 ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt cgctatgggc atttttcggc
    23581 tggtacacct taacaaacgt cgaaaagacc tggtctttcg ccgcgctttg cccgctgggg
    23641 ctggcgttgc tggtaggatt ttcaattcct gacagagtca tggaaggaaa acatccgcaa
    23701 ttttttgtcg agatgacaca agaatcactt cagccaagcc gctatattct tactgatagc
    23761 gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg atgacatcat catgtatcgc
    23821 cagacaggtg agttgaaata cggccttaat tatccggatg cgaaagggag atttgtcagc
    23881 ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg aggggattat tacactcgtg
    23941 ctttcggttg accgcgatga agatatcaac agtctcgcca ttccgcccgc agatgccatc
    24001 gttcgtcagg agcgtctggt gctgattcag tatcgtccca aatgatctgg ctaacattag
    24061 tctttgccag cttgcttagc gttgccgggc agttgtgtca gaaacaggca acctgctttg
    24121 tggcgataaa caaacggcgc aaacatatcg tgctgtggct gggactggcg ctggcttgtc
    24181 ttggtcttgc catggtgctc tggctgctgg tcttgcagaa cgtaccggta ggcattgcct
    24241 acccgatgtt aagtctgaat tttgtctggg tgacgctggc tgcagtaaaa ctgtggcacg
    24301 aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat tattggtggc attgtgatcc
    24361 tcgggagtac ggtgtaatgg gcctgatatg gggattattc agcgtcatta ttgcctcagt
    24421 tgcgcaatta agcctgggtt ttgcggcaag tcatctgccg ccgatgacgc acttatggga
    24481 ttttatcgcg gctctgttag cttttggcct ggatgcccgg atactgttac tcgggctgct
    24541 gggttatctg ctttccgttt tttgttggta taaaacgcta cataaacttg cgctaagcaa
    24601 agcctatgcc ttgctcagta tgagttatgt gctggtatgg attgcttcaa tggtacttcc
    24661 cggctgggaa gggacttttt cgctgaaagc actactggga gtagcctgta ttatgagcgg
    24721 gttgatgctg attttcctgc ccacgacaaa acaacgttac tgagttttcc ctgccgctgt
    24781 acactgccgt tcccactcat cggggctata actgcttgcc gagaggactt tcaccgtatg
    24841 cagcttttca cgattaatac aataaagcgc attctgcaaa ggcgagagta aatcccccac
    24901 tttcacggcg aagtcactct tcacttcggc gatcattttt atcgcgccgc cagcatcgca
    24961 gagcataagt acatgtctgt tatcttgttt attgcagcac gattttttgt gccagccatt
    25021 ccattgcatt atcctgtttg ctaagagttt tcacatcaat ttacagatta acagataaac
    25081 tctttatcaa cggcaatgga atggcagaaa tgagaagtga aacggagggt taattattgc
    25141 tgacgttgca cccactcttt tagcgcctga cgtgaaattt taataccgcc gtttttcagc
    25201 tccggcggta aagtcagcca gcgcaccggt tgttgaaaac gagccagctt atctttcacc
    25261 cattcgccaa gatcaacggt ttgctggtca tactccacca ccgctaccgg ccgatgacca
    25321 aactccttgt cggcaacgga aacgataaac acctgcaaaa ccgcaggatg tgcagcaata
    25381 acacgctcga cttcttccgg ctgaataccc tcaccgccac tgaagaacag attgtctaaa
    25441 cgtccgacaa tggtcagctt gccattatgc atctcaccgc gatcgcgcgt agcgtaccag
    25501 ccttcgtcat taaccagtga aaccagttgc ccgttacgcc agtaaccttc tgccatactg
    25561 gcagcccgca gccacacttc attattaacg attttcactt cccgacccgg cagcggcaaa
    25621 ccaacgtctg ccaggacgtc ggcttctttc gcacataccg tggaggcaaa ctcggtcaga
    25681 ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct gttccgtcaa ctcgaccggg
    25741 atagccgcgc cgccaagtaa caccgctttc agggaaacgg aactgcggtt aaccagcaaa
    25801 cgccagagtt gtgttggcac cagtgaagcg tgagtacagc ctgccagcat ttgctccaat
    25861 ggctgtttat cacgcaccgt catccgcgca ccagcgtata accagcgcca cataattccc
    25921 tgaccggaga cgtgaaacag cggtaaagag agcaaccagt catcgtgatc gccaaacgga
    25981 atcagcgata acacaccttg cgcactggca agatgggcct gataagtatg gacagcggct
    26041 ttcggcaaac cggtagaacc cgaggtcaac gtcattgagc acagacgcgt cggctgccac
    26101 gcagcggcat gtacgccttc aaccagctga atgtgcagcg acgttaatgc cggaaacgtg
    26161 ttttccccat ccggcaccag cgcaaattgc agcgtcagat tgggcagcaa ttcttcaagc
    26221 aacggttgcg gcagctgagg gttcacgggc aacacccgcg ccccgcattg cagtaacgcc
    26281 agccaggcga gcagcgtttg cggcgtattc cacgcccgca acatcacgcc gctgccctca
    26341 accacaccct gcgccgcaaa gcccgtagct aactcatcga cgcgagcaca aagctcgcgc
    26401 cagttgagtt gctcgtcatt aagacgtaag gcgatggctt ctccccgcac ttgccgccag
    26461 tgacgccacg gccagtcaga gaagatcata acaactgctc cagtacatca gcgtccacta
    26521 acggcagtgg gctccccggc cagcgacgaa cctgctgcgc ctgcatcaga tccagcgtgt
    26581 ccagtcccgg aatggtgtct ggcgttaacc aggcagcaat ccgcgccagt tgcgttagac
    26641 ctaagctcga ctcaatggaa gaactgatca ccgccgtcag ccccagcgca tgcgccgcct
    26701 gtacctgctc gcgtactttt tccagactgc ccgtgagcgt gggtttgata actaccgcgc
    26761 gcacgccctc ttcagccaca aaggcaaaat ccggctcgcg caggctttca tcccaggcaa
    26821 tggcaatgcc ggtttcacgg gcaaacgctc gcgaatcatc gcgggttttg cacggctctt
    26881 cgagaaacgc gatgcggtgg cgataatccg ggttaacata tttggcaaac tgctgacctt
    26941 tcagcggtgt ccaggcgcga tttgcgtcaa gacgcaaatg cagatccgga attgcctcca
    27001 gcaacagatt caccaccatg ccgtcgcgca ccgcttcgta caatcccact ttgaccttcg
    27061 ccactttctc gcccggcata tcggcaagtt tgaggatcag atcgtccgga tcgccattac
    27121 acagcggtgc cgcacggtag ttggctgctt gcggcaacgt atctgtcagt tctgccaatg
    27181 cacagcttac gccaaaggcc acggaaggca tctgcggtag ctcgcaatcg cctgccagcc
    27241 agttatttac ccaggcaagc agcacgcttt gcgcctcttc ccagttttcc tggctgaagc
    27301 ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc ctcacgcagg caaacgtaca
    27361 gcccgtcgcg ggtttttaac cgcctgtcgc gcagaagcac ccccgcgtcc atggggatct
    27421 gccagcggta tacctgcgcg ctacgcatta cggattccgt ttaaatttgc tgaaatcagg
    27481 ctgacgtttc tggttgaaag cgttgcgacc ttcctgacct tcttccgtca tgtagaacag
    27541 catggtggcg ttgcccgcca gctcctgcag ccccgcctgc ccgtcacagt cggcgttcag
    27601 cgcagctttc aggcagcgca gcgccatcgg gctgttttgc agcatttcgc ggcaccaacg
    27661 gacggtttct ttttccagat ccgccagcgg taccacggtg ttgacaaggc ccatatccag
    27721 cgcctgtttt gcgtcgtact gacggcacag gaaccagatt tctcgcgctt ttttctgccc
    27781 gacgatacga gccatgtagg aagcgcccca gccgccgtcg aaggaaccga ctttcgggcc
    27841 agtctgaccg aagatggcat tatctgctgc gatagtcagg tcgcacatca tgtgcagaac
    27901 gtgaccaccg ccgatggagt agccagccac catcgcgaca accggtttcg gacaggtacg
    27961 gatctgacgc tggaagtcca gcacgttcag gtgatgtacg ccggaatcat ctttatagcc
    28021 gccgtaatca ccacgcactt tctggtcacc accggagcag aacgctttat cgcctgcgcc
    28081 agtcagaatg atcacgccga tattgtcgtc ataacgcgca tctgccagtg cctggatcat
    28141 ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc ggacgattaa tggtgatttt
    28201 tgcgataccg tcggtggatt tttcataacg aatgtcctcg aaaccttcgg agcagtcgtg
    28261 ccattcaacc ggtgcgtaag gcattgcttc atcaggataa atcatattgt gtcctttagt
    28321 caaaaacgca agatctgcgc cagacttgcg attacgccag cgggattttc ccgatgcgcg
    28381 ttatgtccgg cgcgaggaat gacatggcag tcggcagcca gttccgccgc tagggcgcgg
    28441 aatttgctgt cacgttcacc acataaataa taaaacgcaa atgtgcgggc gctaaggtta
    28501 gcacgtaaat caggctggac ggcgagagaa gtcgcctcca gcatggcggc aagggttgcg
    28561 ccattattgt tgctgcgcag cgccaccagc tcccggcgtt gatcgtcatt gagcgaggca
    28621 aaaacaggct gttgatacca gtcggcaaat accgccgtta acggttctgt gcgaaaacgc
    28681 tgcgcccatt ggcgatcgga acgctgacgt tccgcacgtt gttcagcatt ttgcagcccc
    28741 ggatgcccgc cttcgataat aaccccacaa agccccgcca gcccctggca agccgccatc
    28801 atcgccaccc gtccgccaag cgagtacccc accagccaga agtcaaggat gttgtaacta
    28861 accaaggttt tacgcagtaa gtcggtgaca tcatcaaatc catcgacgct aatcgccgcc
    28921 gaaccaccat gacctgggag atcaacatac aaccgtgagt agtcggcaaa cgcctcgccc
    28981 acttcttgcc attcgtggca atcgccggaa aaaccgtgca aaaacaccag ccagggtaaa
    29041 cctggttttc cgtgttttgc ctgcgcgtgc aggatcataa atggcttacc tgcgccagaa
    29101 gttgctggag cgtttgcgca ccatcggtgt cgttaaccac catttcaatc accgtggtgg
    29161 ttggcgtgcg ccaggcgtcg gcaaatgccg tttcaagttc ctgccagttt tgcggacgat
    29221 gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg gacgttttgc ggcatcagat
    29281 agaaacgctc acgctcgctt tgcggcgttg gcaacagcga gaaaatttgc ccgccgttgt
    29341 tgttcaccac aattaatacc agcggcgcag aaacctgacg caataacgcc agcgcgttga
    29401 gatcgtaaag tgcggagaga tcgcccacaa tcgccagcgt cggtttgccg cttgcccgct
    29461 gaacgcctgc ggcggtcgaa agcagtccgt cgataccgct ggcccctcgg ttgctgtaca
    29521 ccgggtaacc tgccggaagt tgcgaaagcg catcaatcag acgtaccacc aggctgttac
    29581 caacaaacaa ttgcccctgt tcaggcagat agtcgctgat gcgatgcgcc agttgcgctt
    29641 cgccaaacgc atcacggcgg gcaataaccg cctgcattgc ctgttccgcc aggcgcggga
    29701 tttcaacgca ccagggctgg cgtttttctg ccggatgcag ctccagccag tcggcaatat
    29761 tgccaattaa gcgacgtccg cgatggtgtg ccggatcaag tcgcccttca atgtcatcaa
    29821 caatccagta ctcttctggt tcacagcttg cctgccattg caggagccgt ttccccgtca
    29881 ggctgcttcc cagttgcacc acaatttgtg cctgctgcag ctcgctggtc gctttggcat
    29941 tgcctaacca aagatcggca cacggcagcg gctgcccggt ttgtgacagc acatcgccaa
    30001 tcagcggcca gccaagagtt tgcgcccaca gggcaacttt tttgccctct tccgcactca
    30061 tgcgcccggc aaccaccacg ccgcgctttt gtcgccagaa gaaccagtcg cgctgttttt
    30121 cactttccag acgaggcgct tcacgcagcc acggtttgtc gtcctgccac cagtcaccca
    30181 gacgctgttg ccagctaagc ccggtatcgt ccatttcgcc atacagcggt tcagcaaacg
    30241 ggcagttgat atggactccc cccgcatgaa gcgtaccgag agcgtggtcg atggtagaaa
    30301 ccagccaacg tgcggggata tcctgggtcg ggcgcggcaa tgaaatactg tgcgtggggt
    30361 gagaggcgaa cattcccggc tggcgaattg cctgattcgc gccgcagtca attagctccg
    30421 gcgggcgatc ggcggttaag agaatcagtt tttctccggt taacccggct tcaatcagtg
    30481 ccggatagag atttgccacc gccgtgccgg aggtcacaat caccgccacc ggctgcttgc
    30541 tgactttcgc cagccccagc gccagatgcc ccaacccacg ctcatcgaaa tgggtgtggt
    30601 gaatgaatgc ggaattctcc gccgccgcta acgttaacgg tgtagaacgc gagcctgggg
    30661 cgatacagat gtgtctgacg ccgtgacgcg ttaatgcttc cagaatgacc gccgcccagc
    30721 gtcggttaaa tgcgcttact gacatgagtt tgtccggtat caatattgcg gctaagtata
    30781 aggagctaca aaaatagatt attgatatga atcggtaatg atgcgactca ttactattcc
    30841 atttgtaata aagtacgcag ccctgccgct ttgttgtcga tttcctgcca ctcttgctcg
    30901 gggtcggaac cacggacaat gcccgcgcca gcatataatc gcacgacatt gccgctaatt
    30961 tttgctgagc gcagggaaac gcagaattcg ctttgttgta atgagagata gcccgcagaa
    31021 ccggcgtacc attctcgggt gaacggttcg tgacgggcga taaactgtcg cgccagatcg
    31081 cgcggtaagc cagcaactgc tgccgttggc tgcaactgat gtaaacagat cacatcatcc
    31141 gctttgttga gtgaagtcca gatacagcgg cgaagatgct gcactttacg cagacgcagt
    31201 acctgcggcg gtaaaacatc cagcgtctgg gtatcggcct gtaatcgttg gcagatatct
    31261 tccaccacca gcatgttctc gcgctggttt ttatcatccg ccatcagcca ctctcctaac
    31321 tgctgcgcct gcttatcatc aggatgattt gctactgttc ccgccagcgc ttcagtacgc
    31381 agcgctttgt cacgccgccg ccataaccgt tccggtgaag agccaagaaa agcattttcg
    31441 ccatcaaagg ccatgtaaaa atggtagcaa ttcagattca gtcgacgact ggcagccatc
    31501 atcgccgccg cgttgaccgg acttgcgaaa tgcaggtcag ttgcccgagc gagcaccact
    31561 ttgtcgagct caccttcggc gatggttttc gttgccagtt cgattaattg cgtccagccc
    31621 gttttgtccg gccagtgttg ttctcgcgtg gtggttaaat gtaacccagg caagggcttg
    31681 atactcacca gtgtggcgat aaattctttt gcctgaatcg catcgtgctg aagggagctt
    31741 tcgctgaata gcgtcaaccg cagcgtggct ttaccgccac agcgtcgcca ttccaggcgg
    31801 ggtaaaagta aattgccctg cgacgggtca aaagcattca gcccccaaat gcgtaagtcg
    31861 gcgtgttccg ggtgctggcg aagaaaacgt tgtgcctggt ccaacgacgt aaaacgggta
    31921 atcgcgccca ggacggcagc ttcttcatca ccattacgtt gttgccagta gaattgcggg
    31981 tacgtttgct gactggccag ccagctcaag gcatcaaaag cgtctttgag agggaaagga
    32041 atatcgataa cccgaatgcc gggtgtcgcc ggaatctctt gcgacaaatg gcgcagtaga
    32101 ttttccagcg ccgtagtaag tgattgcacg cggacctctc cctgttaaaa gcctcacatt
    32161 atacggggta ctacaaaaaa aatgcagtac cccggtgtag ggaggtttta acggcgtgcc
    32221 agcaacagtc ctagtaccag cccgacggcc gcgcccacac caattccttg ccagggtttt
    32281 tcgtggacgt agtcatcagc acgataaaca gcctgcttcg cccgatagta ataactgtct
    32341 gaagcctggc taacccgttt tttcacatca tccagcgctt tttctgcacg cgctttcagc
    32401 tcaacatatt tctgatcggc gggctcgcca gaggagcgga gcacctcttc cagtgtttca
    32461 ctaagcagcg tcaggtcgtc atcgatacgt gtatcaccaa actgattaga cattctcgtt
    32521 ctccatttgc gtaaaacctg tcactaacta tagacaagag ttgttgatta cgcctgaatt
    32581 acctcgcgtg ccatcccaat gtgtgggata ccatcttctt catagacctc cgtcacaggg
    32641 ataaaaccga aactctggta gaagttttgc agatgcgcct gcgccccgag gtaaacaggc
    32701 ttatcaggcc agtgatgcgt acatgtttcg agtgttttac tcatcagctg ctgccccact
    32761 ttttcaccac gcaacgcttc actgacaatc acccgaccta taacgacagg ctcaagatca
    32821 tcatcacttt tcagaatcct cgcatacgcc accagttcat catttttcca accgagaata
    32881 tggcgattat cccccgtcaa atcatcacca tcgatatcct gataagggca attctgctcg
    32941 acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa gctgggaaac cgataattca
    33001 gagtgatgca gatcttgcca ttcaatcata atgctctcct tttagatgta tgccttatac
    33061 tagccggaat attttttgaa acgtgatgaa ctcatggaat taattttttt aggtacttca
    33121 gccggtgtgc caacccgcac gcgcaatgtc acggcaatat tgcttaacct gcaacatccg
    33181 acccagagcg gactttggtt gtttgactgc ggtgaaggca cccagcatca gctactgcat
    33241 accgccttta accctggaaa actggacaag atttttatca gtcaccttca tggcgatcat
    33301 ctttttggtt tacccggctt gctgtgcagt cgttctatgt caggcattat ccaaccctta
    33361 acgatttatg gtccgcaagg tatccgtgaa tttgtggaaa ccgcgcagcg gattagcggc
    33421 tcatggaccg attatccgct ggaaattgtc gaaattggcg ctggcgaaat tctcgatgat
    33481 ggcctgcgca aagtaaccgc ttatccgctg gaacacccac tggaatgtta tggctatcgt
    33541 attgaagaac atgataaacc gggtgcatta aatgcccagg cattaaaagc tgctggcgtg
    33601 ccgcctggcc cactgtttca ggaattaaaa gcgggcaaaa caatcacgct ggaagatgga
    33661 aggcagatta acggcgcaga ttacttagct gctccagtgc caggtaaagc gctcgctatt
    33721 ttcggcgata ccggcccctg cgatgccgca cttgacctgg ctaaaggtgt cgatgtcatg
    33781 gtgcacgaag cgacgctgga tataaccatg gaagccaaag ccaatagtcg cggccatagc
    33841 tctacacgcc aggctgcgac actagcccgt gaggctggag tcggcaagct aatcattacc
    33901 cacgtcagct cgcgctatga tgacaaaggt tgtcaacacc tgttacgtga atgcaggtca
    33961 attttcccgg cgactgaact ggcgaatgat ttcaccgtgt ttaacgttta actctgctta
    34021 ttattaaaaa cagggtgaaa cttgccctgt tatagcaacc cgcgctacat atatccaccc
    34081 acacaggcat attgagcgat ttttgatatt tattcatctc cttacgcact atattcagag
    34141 caataagttg cctcttttca tcttgattca gcacaacctt tgcaactaaa aggagcccat
    34201 gatggttaca gttgtcagca attattgtca attatctcaa acccaactca gtcagacatt
    34261 tgcagaaaaa tttactgtga ccgaggaatt actgcagtct ttaaaaaaaa cagcgttatc
    34321 cggagatgaa gaaagtattg agttactgca taatattgcg ttaggttatg atgaatttgg
    34381 aaaaaaagct gaagatattc tttaccatat tgttagaaac ccaacaaatg agaccctatc
    34441 gattatccga cttattaaaa atgcctgttt aaaattatat aatttggcac ataccgcaac
    34501 caactcccac cttaaaccaa ctggtccgga taattcagat gttctcctgt tcaaaaagct
    34561 attctcccct tcgaaattaa tgacaattat cggtaatgaa attcctctta tatctgaaaa
    34621 acagtcgctt tcaaaggtgc ttttaaatga tgagaataat gaactgagtg atggtacaaa
    34681 cttctgggat aaaaatcgtc aattaaccac agatgaaata gcttgctatc ttcagaagat
    34741 cgccgctaat gcaaaaaata ctcaagtcaa ttatcctact ggtctctacg tcccctactc
    34801 caccagaact cacctggaag acgctctcaa tgaaaatatt aagagcgatc catcatggcc
    34861 gaatgaagtc cagttattcc ccataaatac tggcggacac tggatattag tttcgctaca
    34921 gaaaatagta aataaaaaaa ataataaact acaaataaaa tgcgtcatat tcaactcatt
    34981 gcgtgcacta ggccatgata aagaaaattc acttaagcgt gtcattaata gttttaattc
    35041 tgaactcatg ggaaaaatgt cgaataataa tataaaagtt catttaaatg aaccagagat
    35101 aatattttta catgccgatc ttcagcaata cttaagccaa agttgcggtg cgtttgtgtg
    35161 catggcagcc caggaagtga ttgaacaaag ggaaagcaat tctgacagcg ccccctatac
    35221 gttattaaaa aactatgctg acagatttaa aaaatattca gcagaagagc agtacgaaat
    35281 tgattttcaa catcgactgg taaacagaaa ttgttattta gataaatatg gcgatgcacg
    35341 aatcaatgct tcttatacac aattagaaat aaaacactca caacccaaaa atagagcatc
    35401 cggcaaaaga gtgagttaac atcattgtta atcaattcga ccagtcagta aatatgctga
    35461 ctggtcgaac ataatttgct aaaaagtcat cactgactga tgtcagtccc accatctgcc
    35521 agttcaatct acgggtattt ccagcgtatc gcggcacgcg attgctggtc tgttgatgta
    35581 gtggattgta gttgctgact tttcttaccc tcagatttct gccccacttg tcaattcaat
    35641 ctgccaataa ataacataat tatattttta ttcctcacag aaattccttt ttaaaaatag
    35701 cgaacccact gtaaaaaaca ctttttgtat acctctacca agcgtaataa aataaacgct
    35761 cataattcat tataaataat aatgaaataa cgccttttat tgataattta catatgaaaa
    35821 aaattttcct tttgttatat tatttctgtt ttcactccct ctgattattt ttaccaaccc
    35881 tgggtcactg cattatcacc aacgccccga cacgtaagtc ctgagcaatt agaaaaaaca
    35941 gttcgttatc ttacacaaac tgtgcatcca cgtaatgccg gcaatattga taatctgaat
    36001 aggtccgcag agtatataaa agagttcttt gtcagtagcg gtgccagagt taccgcgcag
    36061 ggcgtccccg attggctatt acgactctgc gcctggaagc cagaattacc cttacccggc
    36121 aatgtcctgg ctttatcccg atcagggaga ctttattgct gtggtcggca gaatgcagga
    36181 tatcaacgcc gttcgtcagg taaaagcggc attgttgtca tcccgggatt tatctgttta
    36241 ttctatgaat gccccagggt ttattcccgg tattgatttt tctgatcacc ttaattattg
    36301 gcaacacgat attcccgccg taatgattac tgacaccgct ttttatcgta ataaacaata
    36361 ccacttgccc ggtgataccg cagacagatt gaattatcag aaaatggctc aggtggtaga
    36421 tggtgttata accttgttat acaacagtaa ataataaaaa tttctcgagg cgggtccaga
    36481 aacagcgaca tgtacatttt attttccctc gtctgtttta agatgcgaaa cataaaattc
    36541 aatggaggaa aaataaatgg gaatgattgg ctattttgca gaaattgact cagaaaagat
    36601 taaccaactg cttgagtcta ctgaaaaacc tttgatggat aatatacatg acacgctttc
    36661 aggattacgc cgactggata ttgataaacg ctgggatttt ttacactttg ggctgacagg
    36721 cacctctgct tttgatcccg cgaagaatga tccgctgagt cgtgccgtgc ttgatgaaca
    36781 cagcctcgaa gatggcatag atggcttcct gggattgacc tggaatcagg aattagccgc
    36841 gacaatcgac cgactggaat cactggatcg aagtgagctg cgtaaacagt tttccattaa
    36901 gcgactcaat gaaatggaga tttatccggg agtgaccttt tcagaagagt tagaggggca
    36961 actctttgcc tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc gacgtatgct
    37021 gcgacaaggg aatcatgcgc tgacggttat tgttgggtga tggtgtcaga gcgccaaaaa
    37081 aaaccgccga acatgtcggc ggctttctga cttacaaact aacagtttac atcagcggca
    37141 ttgctaaccg cacaatacta atcagcggtt gtggccatac acccagcacc agtaccaaca
    37201 gtgcagaaat cagcaccacg ataccgcccg cgctgtactg ccagtttgac ggtgcatcgc
    37261 gacccggttg ttccggtgcg tgcaggtaca ggctcaccgc cacgcgcagg tagtagtaga
    37321 ggccgattgc cgaaccgaca accacggcac ccaccagcca ccacaagtgt gcctggacac
    37381 cgaccgccag cacgtagaac ttaccgataa agcccagcgt catcgggata ccggccagag
    37441 acagcatcat caccgtcatc actgccgcga ggatcggacg atgccagaac agaccgcggt
    37501 aagagaacag ggaatcagca tccgggccac gatacgggct ggacatcagg ctgaccacgc
    37561 cgaacgcgcc gaggctgctg aacagataac cggccaggta aacccctacc gcttccatcg
    37621 acatctcgcc ggtttgcagc gcaatcagcg ccaccagcag atagccgagg tgagagatag
    37681 atgagtaacc gagcagacgt ttgatgttgg tctggctcaa cgccatcagg ttaccgaaga
    37741 tgatggaggc aaaggcgata atcgccagta ctacgcgaat cgcttcgctg tcacccaccg
    37801 gtgcgtagag gaacagacgc atcaccacac cgaagatagc gattttgctc gccgtcgcca
    37861 ggaaagtgga aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac aggtggaacg
    37921 gcaccagaga gagtttgaag ccgaggccaa caatcatcat gccgaaacct gccagcaaca
    37981 gcggctcgtt gagcataccg tcgccgaggt ttttacccag cgcgacaaac gacaggtcgc
    38041 cagactgcgc atacaccagc gccataccga acagcaggaa agaagacgct gcggcagaaa
    38101 ggatggtgta tttgatactg gcttccagcg aacgtttctg gcggaaagcg taaccgacca
    38161 ggccaaacag cggcaaagag atcagttcaa taccgaggaa cagagacgcc agatggtttg
    38221 cattcgccag caggatcccg cccagcgcgg caattaacac caacaggtag aactcatcct
    38281 tgttgtcgtt atagccttca agccacgggt agtcgaaagt acaggtggcg aggctcgcca
    38341 acaataccag cccggtgtaa agcatggcga aaccatcaac gcgcatcagc ggcgtaacgt
    38401 ccatagcgcc cgcctggcca acaaaccaga gcgaaaccag cgccgcgtta agcccaataa
    38461 ccgagagcgt agcgttgagg aaatgattgc gtcgccacgc aatggagagc atcacaacca
    38521 ccaccgtcaa gccgacgatc agcaacggta gcagtgcgat caggttttgt ggagttattg
    38581 tcatggcgat ttacggcctt gtagtagtaa cggaattaac aaaccactgc tggatattgc
    38641 caatcgcgga gtgcgaggta tccagaatcg gctgcggata gaagcccagc agtaccagca
    38701 gcaccaccag caacaggatc ataaacagct cacgcagcga catccctggc agttcctggc
    38761 tggcaatctg gcttttcgct ttaccgaagt aagcgcgatg caacatcgcc agcgaataaa
    38821 cagatgcaaa gaccagccca aaggtagaga taacggtaat caccgggaca acctggaagc
    38881 tgccgaacag aatcataaat tcgccgacga agttaccggt gccaggcatc ccaagcgttg
    38941 ccaccgcaaa gaacagcgac agtgctggca gccatttcat cttgctccac agaccgccca
    39001 tcatgcgcat gtcgcgggta tggatacgtt cataaagctg accgcagaga ataaacagac
    39061 ccgccgccga caagccgtgc gcaatcatct ggattaccgc gccctggtag gccaactggc
    39121 tgccggtgta gattgcaatc agcacgaagc ccatgtggga aaccgaggtg taggcgatca
    39181 gacgtttgat atcggtctga gcgaaggcca tccacgcacc gtagaagatg ccgataacac
    39241 ccagccacat agcgattggc gcgaactctg ccgacgcgtt cgggaacagc ggcagggaga
    39301 aacgcaacaa accgtaagcg gcggttttca gcaagatccc cgcgaggtcc acggaaccgg
    39361 cggtcggagc ctgggagtgc gcatccggca gccagccatg cagcggaacc accggcattt
    39421 tgactgcgaa ggcgatgaag aagcccagca tcaacagata ttccacacca ctggacattg
    39481 gcgtattcag cagctcttca tagttgaagg tccagacgcc ggtcgcattg tagtgaacaa
    39541 aaaccagcgc caggatggcg atcaacatca ccagaccact cgcctgggtg taaatgaaga
    39601 acttggttgc caccgtgata cgcgttttac cgtcagaggc tttatgcccc cacagtgcga
    39661 tcaggaagta catcggcacc agcatcattt cccagaagaa gaagaacagg aacatgtcga
    39721 tggcaaggaa cacgccgata acgccgccca ggatccacat caggttgagg tggaagaagc
    39781 cctgatattt ttcgatctct ttccacgaac atagtaccgc cagcacaccg agcagaccgg
    39841 tcagcacgac catcagcagc gacagaccgt caatggcgag atgaatagag ataccaaaac
    39901 gcgggatcca cggcatgtcg aattcagact gccactgcgg aattccggcg gattgcgtca
    39961 gtgaataacc gccctgcaac cacagttgca gcgacagcgc cagcgtcaat cccatggtga
    40021 tcagcgcgat ccagcgtggc accttgacgc caaagcgttc ggtctgccag cacaggaagc
    40081 cgccaataaa gggaattaat attagccagg gtagtaacat ggcgatcttt attccttgta
    40141 aaagtcccgt caggaccgga ttttcaacga attctcacga cagaaaattc tcttcggact
    40201 gggggtagtg tcggatgcgc cgcgaaggca gcttatccga cctacgttct tatatgccca
    40261 ggggcaatcc cacaatcctt aactcaacgc agtaccatca acagtgccag caccacgacc
    40321 gcaccgatgc tcatggatgc cacataccag cgcagataac cgttctcgct taacagcaga
    40381 cctttacctg caaagcggga aagaaccgcc gggatgttca tcattgagtt cagcggatcg
    40441 cgtttcagca accaggcaat acccaggaac ggcttgacga acactttgtc gtacagccag
    40501 tcaaagcccc aggcgttgta ccaccaggta ctcagcaggc ggcccggcgc actgttggcg
    40561 atggaggtca ccagagtacg tttacccagc cacagccagg ctgccagcag aatgccgacc
    40621 accgcgacca cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc cagttccgtc
    40681 gtttgcggaa gcacgccctg cagcggcggt acaatcagtg cgccaacgaa ggtggaaagg
    40741 atcagcagca caatcagcgg caggctgtga gttacccctt tcacggcgtg agcgtgaatt
    40801 tgttcttttc cgtggaagac gatgaaaatc atacggaagg tgtagagcga ggtcataaac
    40861 gcaccgacca gacctgccac catcagattg atatgaccat tcgccatcgc acccgcgagg
    40921 atctcatcct tactgaagaa gcccgcagtg accagcggca gtgccgacag tgctgcgccg
    40981 cccaccagga agcagagata aaccagcgga atagatttac gcagaccgcc catcttgaag
    41041 atgttctgtt cgtgatggca ggccagaatg acggaaccgg atgccaggaa cagcagcgct
    41101 ttaaagaacg cgtgagtcat caagtggaaa atcgccgcat cccatgcctg cacgccaagc
    41161 gcgaggaaca tgtagccaat ctggctcatg gtagagtaag cgagaacacg tttgatgtcg
    41221 gtctgtacca gcgcggcaaa accggccagc agcagcgtaa ccgccccgac aatacccacc
    41281 agatgcagaa cttccggcgt catcaggaac aggccgtggg tacgggcgat caggtagaca
    41341 cccgcggtta ccatcgttgc ggcgtggatc agcgcggaga caggcgtcgg gcccgccatc
    41401 gcgtcagcaa gccatgtctg caacggcaac tgcgcagatt taccgaccgc accgcccagc
    41461 agcatcagcg tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc tggtgccagt
    41521 tccaccattt cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa cagtgcgaaa
    41581 gcgaggaaaa cgtcacccac acgggtcacg acgaacgctt tcattgctgc tgcgccattc
    41641 ttcggatcgg tgtaatagaa accgatcagc agataggagc acaggcccac gccttcccag
    41701 ccgaggtaca tcagcagcag gttatcggcg agaaccagaa ctaccatgct ggcgatgaac
    41761 aggttggtgt aggcgaagaa gcgagagtag ccctcttcac cgcgcatgta ccaggaggcg
    41821 tacatgtgaa taaggaaacc cacaccagtg accaccgaga gcatggtcag cgacaggccg
    41881 tccagcacca ggttaaaacc gatgttaaag tcgcctaccg acatccacgt ccacagcggc
    41941 tggctgtatg cctgctcgcc gttagcgaag aaatcaacgc cgataaaggc ggttacctgc
    42001 gccgccaggc ccacagagcc aacgccgacg atcgccgaaa cgttttcaga ccagcgccca
    42061 cgggagaacg ccagcaggac gaagccaatc aatggcaaaa taatggttaa ggcaagcatg
    42121 ttcatccgcg catctcactt actgaatcga tgttcaggtt ctggcgacga cggtgaagtt
    42181 gcagcagcag cgcaaggccg atactcgctt ctgccgccgc gaggctgatg gcgagaatgt
    42241 acatcacctg accgtcggtc tgaccccagt agcttccggc gaccacaaag gccagcgcgg
    42301 aggcgttaat catgatttcc agaccaatca gcataaacag cagattgcga cggataacca
    42361 gaccggttaa gccaagaacg aataagattg ccgcgaggat cagtccatgt tgtaagggga
    42421 tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct ttacgattgc tcagcacttc
    42481 acccgcacgc tcttcacgac cgacgtggaa ggccacaacc agacccgcga gcagcagcat
    42541 agaagccagt tccaccgcca gtacgtaagg cccgaacagc gtaataccca ctgctttagc
    42601 actgattggc gtaccgtcga taccctgatc gttaacaccg aggatggcgt aaacaatcac
    42661 caccagcatg atggccgaca aaattgccgg gccaatccac acctgcggtt tcagccactg
    42721 gcgttcctgt tcgatttctg aaccgcccag gttgagcatc atcaccacga acacgaacag
    42781 caccataatg gcacccgcgt agacgataat ttccagcgca ccggcgaagt aagcgcctag
    42841 tgagaagaac accccggaaa tcgccagcag cgaaataatc aggtacagca gtgcgtgtac
    42901 cggattggta tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc cacagatata
    42961 aaaagcgaac tccattgccc ctctccttac ggtaacaggc tcttgacgtc gataggcttg
    43021 gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc catccggtag
    43081 aagttatatt ccgggtattt gcccggaccg gagatcagca gatcctcttt ctcgtaaacc
    43141 agatcctggc gcttgtattc ccccatttcg aaatccgggg ttaactgaat cgccgtggtc
    43201 ggacaggctt cttcgcacag accacagaaa atgcagcgtg agaagttaat gcggaaaaat
    43261 tccggatacc agcgaccgtc tttggtttct gctttttgca gcgagataca gccgaccggg
    43321 caggctaccg cacagaggtt acaggctacg caacgctctt cgccgtccgg gtcgcgggtc
    43381 agaacgatac gaccacgata acggggcggc agatagaccg gctcttccgg gtacattcgc
    43441 gtttcgcgtt tggcgaacgc gtgcaggccg atcatccaga tactacgaac ctgggtgccg
    43501 aaacctacta acaattcttt taaggtcatg gtcttattgc cccttattgc gcctgccaga
    43561 gaatgacagc cgccgttacc agcaagttga tcagcgtcag cggcaggcag attttccagc
    43621 cgaaggacat tacctggtca taacgcggac gcggtaacga cgcacgaatc aaaatgaaca
    43681 tcatcataaa gaacgcggtt ttcagcgcga accagatgaa tggcggtaac aacgggcctt
    43741 gccagccacc gaagaacagc gtcaccatca atgcagagat ggtcacaatc ccgatgtact
    43801 cacccacgaa gaacagaccg aacttcatac cggaatattc aatgtggtaa ccatccgcca
    43861 gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg acacaccgcc acgcccgcga
    43921 tggcaaaggt aataaaacca aagaattgcg ggatgacgtt ccacacatgc gcctggctgt
    43981 tgacgatgtc ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc agggaaagcc
    44041 cgaggaacac ttcgtagctc agggtctgcg cagaagcacg catcgcaccc agcagcgagt
    44101 atttgttgtt acttgaccag cccgcaaaca gcaccgcgta aaccgccaga cctgccatca
    44161 tcaggaagaa caaaatcccg atgttcaggt cggcaaccac ccaacccgga ctgactggca
    44221 caattgcaaa ggccagcagc agcgaggtaa aggcaatcat cggtgccagg gtaaagatga
    44281 cgcgatccga gaatttcggg atccagtctt ctttaaagaa cattttgatc atgtccgcaa
    44341 ccagctggag cgaaccgccc cagccaacac ggttaggtcc ataacggttc tggaacagac
    44401 ccagcaggcg acgttcgcca aagctcatga atgccccaca ggtgacaacc accagcagga
    44461 tccccaccgc tttgaggatg gtcagcagga tctcaatcag ttccggtgat atccaactca
    44521 ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc agcaccggag caatgccgga
    44581 catacccatc ggcaagccca cctgccctgc cgtcagtcct tcggcgattt caaccggcag
    44641 cgtgaccgta ttgccatcgt aactaaagga gacgcgtgta cctgcgttca cacccaactt
    44701 cgcggcatcc gctgggttga gtttgatgta cggctgcggc atacggctct ggaagaccgg
    44761 agcacgctgt gacaattcat cgctgccaaa caggtggtag tacggcgcga tacgccattt
    44821 cccgtcctgc ggctggaagc gtgccggtac gctggtgaag taatccagac cgttttcgct
    44881 ggtttcaaac agacgcacgc ccggatcgcc aaagcgcagt ttgccgccca cttcgtcctg
    44941 gaatttgttc cacgcctgcg gggagttcca gcccggcgcc caggcaaacg gcacttgcga
    45001 acggtgcgcg gtcggctggt tgttaccttc catcgagaag gtgaacatgg tgtcaatatc
    45061 ctgcggctga cgcggctcat gaacgctgat attggcgcgc atggcggtac gaccgctgta
    45121 acggtgcggt tcacgggcca gtttctgccc acgaatacgg aatgtcgcat ccggcgcagc
    45181 atctttgata cccgccagtt ccgggatttt cgccacaaca gcgtcaatta catggtcgag
    45241 ctgcgtccag tccacttcac ggctcagcaa ggtgctgtgc agcgagtgca gccagcgcca
    45301 gctttccagc atgacagttt tgctgtcgta ataagccgga tcgtaaacct ggaagaaacg
    45361 ttgggcgcga ccttcattat taattaccgt accgtcgctt tcagcaaagc tggcagcgga
    45421 aagcaccaga tgggcgtttt ccatgatcgc tgtgcgttga tgatcaacca ccatcaccag
    45481 cggcgcttta gccagcgcag cattcacgcg ggtagcagaa gcgtaacgat gcagatcgtt
    45541 ttccaacacc accaccgcgt cggcgcgtcc ggtttccagt tcggttaacg cttcttcaag
    45601 cgaaccgcca cccataatgc ccagccccat gctgttgacg gaacgggcaa tcatggtgat
    45661 accaacgtca gcgccgcgac ctttcagggc tttcgcgacg ttagccgcag cctgaatcac
    45721 ctctatgcta ccggcgttcg tcccggagat aatcaacggt ttcttcgcac ctgccagtgc
    45781 ctgcacgatg acgtcgattt tgttttgcag ctcaggttcg ataccgtcaa ccgctggtgc
    45841 ggagttatcc agcgcatggg cgatggcaaa acctaaacgc gcctgatctt caaccggtgc
    45901 gcggtaagtc cacgccgcga tatcatccag acgagtgtca tcaacgttgg taacaaacag
    45961 cggatgcttc gcacgttgac cgatgttgag gattgccgca atctgccagt cagccacttt
    46021 ctgtgctgca gccatttcgc gcgctttacc tttcaccgcc tgacgcactg ccagtgcgac
    46081 gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt accgcatcgt aagattcgat
    46141 ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagc actttcagcg ccagttgcag
    46201 acgttcctgc tcaccgtgag cgataccggt gtagaagttt tcttcgccca ccagttcacg
    46261 cagcgcaaag ttgctttcca cgctggcacg cggagaacca ataccgatca ctttcttcga
    46321 ctgacgcaga atatctgccg cgccctgcat tgcctgttcg gcgttgaggg taatgaaatc
    46381 atcgccacga cgctgtactg gctgacgcgg acgatccttc aggttgacgt aaccgtaacc
    46441 gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac ggttttcgat
    46501 acgacgcagt tcgccgtaac gttcaccggg gctgatgtta cagccgatgg aacattgctg
    46561 gcagatgctc ggcgcaaact gcatatccca tttacggttg taacgctcgg agtgcgtttt
    46621 gtcggtaaat acgccggtcg ggcaaatttc gaccaggtta ccggaaaatt cgctttccag
    46681 cgtgccgtct tccgggcgac cgaagtagac gttgtcgtgc gcgccataaa cgcccagatc
    46741 tgtaccgtca gcgtaatctt tgtagtaacg cacacagcgg tagcaggcga tgcagcggtt
    46801 catttcgtga gagatgaatg gccccaaatc ctgattacgg tgggtacgtt tggtgaaacg
    46861 gtagcgacgg aagctgtgtc cggtcatcac ggtcatatcc tgaagatggc agttaccgcc
    46921 ttcttcacat accggacagt cgtgcgggtg gttggtcatt aaccactcga ccacactttc
    46981 acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg aagccggtgt
    47041 catacaggac atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt gcttcaccgc
    47101 acactggcgg caagcaccga cgcttcccag cgccggatgc cagcaaaagt aaggaatatc
    47161 aaggcccaga gacagacaag cttccagcag gttgtccgct ccgttgacct cgtattcttt
    47221 gccgtctaca tgaattgtag ccattagcat gcttccagtt ttctcggtca gggaccgagc
    47281 gttaatcgaa attcggttac cagcgctctt tcagcaggtt cggctgaatc ccattaatca
    47341 aatgggtatt gctgaacggc tgtttgattc ccgcctcaaa ttcttcgcgg aaatatttga
    47401 tggcgctctg taacggctcc actgcaccag gtgcgtgggc acagaaagtt ttacccgggc
    47461 ctaagaatcg acacagttgc tcaagtgttt cgatatcgcc cggctggcct tcgccacgct
    47521 ccagcgcacg cagaattttc acgctccacg gcagaccgtc gcggcacggc gtacaccagc
    47581 cgcaggactc acgggcgaaa aactcttcca ggttacgcac cagcgacacc atgttgatct
    47641 catggtcaac cgccatcgcc agcgccgtac ccagacggct gcccgcttta ccgatacttt
    47701 cgaattccat cggcagatca aggtgcgctt cggtcaggaa gtcagtcccc gcgccgcctg
    47761 gctgccaggc tttaaatttc agaccatcac gcataccacc ggcgtaatct tcgaggatct
    47821 cgcgtgcggt ggtgccgaac ggcagttccc acagtcccgg atttttcacc cgaccggaga
    47881 agcccatcag cttggtgcca gcatctttac ttttcgagat gttctgatac cactccacgc
    47941 cgttagcgag gatcgccgga acgttacaca gggtttcgac gttgttgaca caggtcggtt
    48001 tgccccatac gccggaggtt gccgggaagg ggggtttcga gcgtgggtta gcacgacgcc
    48061 cttccaggga gttgattaac gctgtttctt ccccgcagat gtagcgccct gccccggtat
    48121 ggacgaacag ttcgaagtcg aaacctgttc ccataatgtt tttgccaagc aagcccgctt
    48181 cagtggcttc ggcaatagcg cggcgcagat ttactgccgc ttcgatatat tcgccacgca
    48241 ggaagatgta gccacggtaa gctttcagcg caaacgcgga gatgagcata ccttccacca
    48301 gcaggtgcgg cagttgctcc atcaacaggc ggtctttata ggtgcccggc tccatttcat
    48361 cggcgttaca cagcaggtaa cggatgttca tggattcgtc tttcggcatc aggctccact
    48421 tcaggccagt cgaaaagccc gcgccgccgc gccctttcag accagcgtct tttacctgat
    48481 taacgatttc gtccggagac agcccggtca gcgccttacg cgccccttcg taaccgtttt
    48541 tgctgcggta ttcgtccagc cacactggct gtttgtcatc gcgcagacgc caggtcagcg
    48601 gatgcgtttc gggagtacgg ataatgtttt tcatttatac cgctccagca gttcagggat
    48661 cgcttccggg gtcagatgcg cgtgagtgtc ctcatcgatc atcatgtttg gccctttatc
    48721 acagttcccc aggcagcaag ttggcagcag cgtaaagcgg ccatcaaatg tcgtttgccc
    48781 tggtttgatg ttcagctttt tctcgagcgc cgcctgaata ccctgatacc cgttgatatg
    48841 acagaccacg ctgtcgcaat aacggatcac atggcgacca accggctggc ggaagatctg
    48901 actgtagaac gttgccacac cttcgacgtc gcttgccgga atacccagca catcggcgat
    48961 cgcgtggatc gcaccatccg gcacccagcc acgctgcttc tgaacgattt tcagcgcttc
    49021 aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg tgctcaatcg cttcacgctc
    49081 tgccgcactc agctcaaaag cctcggtttg tggttgttga ttctcgtgca taattagcgg
    49141 tccacatctg acataacaaa atcgatactg cccagataaa caatcaggtc agacaccagg
    49201 ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt gcggatacga
    49261 gtgcgataac tcatggtgct accgtcgctg gtcaggtagt aactgttgat ccccttggtc
    49321 gcctcaatca tctggaaaga ttcattggca ggcatcaccg gcccccacga cacttgcagg
    49381 aagtgggtga tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg cggcgtggtc
    49441 agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg ctcaagaatg
    49501 cgcagactct ggcgcagctc ttccactttc agcattacgc gggtgtagca gtcagaaaca
    49561 ccaccaccaa ccgggatttc aaagtcgaag ttttcatagc cagaataagg acgcgcctta
    49621 cgcacgtcga agtcgatccc ggtagcacgc aggcccgcgc cagtggtgcc ccactccagc
    49681 gcctctttcg cgccataggc ggcaacgccc tgggtacgac ctttcagaat ggtattttgc
    49741 agcgccgctt tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa ctcacgcagc
    49801 agacgatccc agccgcgcgg caggtcgtgc gctacgccgc caatacggaa ccacgccggg
    49861 tgcatacgga aacccgtgat cgcttccacc agatcgtaaa ttttctgacg atcggtaaag
    49921 gcgaagaaca cgggcgtcat tgcgccgacg tcctgaataa aggtcgagat gtacagcagg
    49981 tggctgttga tacggaacag ttcggagagc ataacgcgaa tgacgttaac gcgatccggc
    50041 acggtgatcc cggccagttt ctctaccgcc agcacgtaag gcatttcgtt aacgcagccg
    50101 ccgaggtatt caatacggtc ggtatacgga atgtagctgt gccaggactg gcgctcgccc
    50161 attttctccg caccacggtg gtggtagccg atgtctggta cgcagtcgac aatctcttcg
    50221 ccgtcgagtt gtaaaacgat acggaaagcc ccgtgcgccg acgggtggtt cggaccgagg
    50281 ttgaggaaca tgaagtcctc gttttcggtg ccgcgcttca tcccccactc ttccggtttg
    50341 aaggtcaggg cttccatctc cagatcctgt ttggctttgg tcagctcaaa cggcgagaat
    50401 tcggtagcgc gcgccgggta atctttacgc agcgggtgac ctttccaggt ttgcggcatc
    50461 atgatgcggc gcaggttcgg gtgaccgtcg aaagtaatgc caaacagatc ccaggtttca
    50521 cgctcatacc agttagcgtt cgggaacagt ttggtgaagg tcggtacgtg caggtcgttt
    50581 tctgccagcg ccaccttcag catgatgtcg cggttacgat cgatagaaat cagatggtag
    50641 aaaacggaaa aatccgcggc aggtaaccct tcgcggtgtg tgcgcagacg ttcgtccatg
    50701 ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt tctttaagaa atcgccaact
    50761 tccagtaatt gttcacgctt gatccacaca acgggaaccc cggtgcgagt cgcctgaaca
    50821 gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa ttaccggatc atcaagatga
    50881 tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcgg tcatattgtt caccattgca
    50941 aatggtccgt ggtgactgtc ggcgcagtaa gcttcgcgaa atagatttag gaataagcga
    51001 agaaaaatcc ctttgccgac aggcgcaaat taaatctcgt caggtgtacg caggttggtt
    51061 acggcaatgc gttcaccgcg cttgcgttcg cgctctgatt gcatattggc gcgataaacg
    51121 ccctgatcgc caaccaccca ggagagcgga cgacgttctt tgccgataga ttcctgcaac
    51181 agcatcagtg cctgcatgta cgcttcagga cgcggcgggc agcccgggat atacacatca
    51241 accgggatga atttatcgac gccctgcaca acggaataaa tatcgtacat accaccagag
    51301 ttggcacagg cccccattga gataacccat tttggttcca gcatctggtc atacagacgc
    51361 tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag gtcagcctga
    51421 cgcggcgaag cacgcaatac ttctgcgcca aaacgcgcca cgtcatgcac cgcggtaaac
    51481 gaagtcacca tctcaacgta acagcaggaa agaccgaagt tatacggcca aattgagttt
    51541 ttacgacccc agttaaccat gtcattgagc ttgcccataa acacgttttt gttaacttct
    51601 tgctccagag ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc gttctcaccg
    51661 ttgggatcta tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta gcgttgacga
    51721 ttagcgatac tgttcgtttc cgggttcata cactcgcggc gtgaacgcgc gggcgtccag
    51781 tccagcgcgc caatacgcac cagataaacc agacctgcca gtaacacaaa aataaaaatt
    51841 gcagcttcca caaagcctac ccagccgctt tcgcggatag aggttgacca tgcgaacaga
    51901 tacagcgctt caacgtcgaa gataacgaag aacatggcca ccagataaaa cttggcggac
    51961 aggcgtaagc gggcggagcc gaccgaatcg ataccggatt caaacggcac gtttttcgac
    52021 ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca acacaggcca
    52081 atggcaacga taagaaagat agcgaatgcc cagtgatgag cgatgacttc agtggatgtt
    52141 gacatactca ttgcttactc atcaaaagta gcgccagatt cactgctctt cacggcagat
    52201 ggacgccaca tcgattcatg gggaggaata aaaaaaacct tacaatcact gtagaaattc
    52261 ttttatacag ctaattgatg tggtctttta ctcctttcta taaccttttg tcaactttaa
    52321 caaaagtttc ttcacattag tttacataat atcaacacca ttagcattta atgccctttc
    52381 accccagatc cttgacgact ccaggataat tagatgttgt tgaatcgtgt ccgttgtgaa
    52441 gcaatggaaa aaatacgggt ctattttgac aggaatttgt gtcgattcct ccccccaaaa
    52501 gagagtattt tcttgatctg tgacacgctt ttgttattcc ataacaaaaa cgcagcaaca
    52561 aatttacgta ttttttaaca tcattgtagc aggtgatttt tttcaggcga ttatttgtgc
    52621 gttcgggacg tgaatctctg gtgattgaaa aataaacagt tttgtacgtt ctgcactatg
    52681 cgaggaagga tttttactaa aaaaaagccg ctggggttta aaacaccccc agcggctcgt
    52741 tttttacact attgtctcag gaattatcta tcgtccgtcg attactcgat atccctttca
    52801 atcaacaatg aatcatcccc ttccggagca gacattgggc tgtattgcca cggattatgg
    52861 tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga cgggtcatag
    52921 cacagcaaat attcggtatc aggtagcggc ggtaagccat ctactccact cagcacacgc
    52981 aggtccgggc tcatcatctc aaccggcctt gccgtcacgc caagaccggc tttcactgcc
    53041 gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc gccatggaat atctgcttta
    53101 ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat catccagcaa tacaagaggg
    53161 atcggctcgc ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt tggcgaggta
    53221 cgcaggttca atgccttaaa cgctgaagga cgatgggtgg ttaccatcaa atccacttcc
    53281 tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc cagcgctaat
    53341 ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc ggcagattca
    53401 tctgaagcac cgatagttaa cacgccctga agattactga acattaatga tgagcaggcc
    53461 tcatcattaa aacgcaggat tttcctggcg taaccaagaa gttgaatgcc atgttcagtt
    53521 aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg ttccagacgc
    53581 tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc ggcagcaaaa
    53641 gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag gttaattatc
    53701 ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga gctggttaat
    53761 gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca gtttcatccg
    53821 gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact cacattgttc
    53881 cgttataatg cctgaagtag atcacagaat atatcttcag ggatcgcata tctattaagt
    53941 tactcactct tttctattta tgacatgcgc gtgtttgtat aaatgtaaat gtgagtcctt
    54001 gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag caatggtgaa
    54061 gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa accttttatt
    54121 tataagggtc attgcgaatt atctgatgca aagttatgtt atgttatgtt atgttatgtt
    54181 atgttatgtt atgttatgtt atgttatgtt aggcaaagta atcttctatt attaataagc
    54241 acatcaaaac ctttttgaat attaaataat aattaattag catcatcctc attcattaat
    54301 tccgcttaac aatagtttca caccttatcc ccattgcgac acctcggaaa tcatcacgct
    54361 gttaaactct ccgtaatgcg gaaatatcat catcgcgttt cattagtgaa ttcttaaatg
    54421 aggcattttc acacaattat cttacagata aaaaaaccag acttgcaatt aagaattaga
    54481 acaattcact atataacatt acatgtaaag catatacacc tcattatttt gtcattatta
    54541 agttattaac agcacaatcg agccttcccc tctggcaaaa tcttattctg cagaccttca
    54601 aaacaccgtc ctgggggagt acattgttct aagctgactt ccacggcagg gagtggcgat
    54661 aacagcaaaa aaggtcaaga ttcatgtccc ccattgaaaa atccagcaaa ttagagaatg
    54721 tctgttatga catccgtggt ccggtgctga aagaagcaaa acgcctggaa gaagaaggta
    54781 acaaggtact gaaactgaac atcggcaacc cagccccatt cggttttgac gcgccagatg
    54841 aaatcctcgt tgacgtgata cgcaacctgc ctaccgctca agggtattgc gattccaaag
    54901 ggctttactc cgcgcgtaaa gccatcatgc agcactacca ggctcgtggc atgcgtgatg
    54961 ttaccgtgga agatatttac atcggcaatg gtgtatcgga gcttatcgtt caggcaatgc
    55021 aggcattgct gaacagcggg gacgaaatgt tggttcctgc accagattac ccactctgga
    55081 ccgcggcggt ttcgctttcc agcggtaaag cggtgcatta tctttgcgat gaatcctctg
    55141 actggttccc ggacctcgat gatattcgcg ctaaaattac gcctcgtacg cgtgggattg
    55201 ttattatcaa cccaaataac ccaaccggcg cggtatattc caaagagctt ttaatggaga
    55261 ttgtggagat tgcacgtcag cataatctca ttatcttcgc cgatgaaatt tatgacaaaa
    55321 ttctctacga cgatgctgag catcactcaa ttgcgccgct ggcacctgac ctgctgacca
    55381 ttacctttaa cggactgtcg aaaacgtacc gcgttgcagg cttccgtcag gggtggatgg
    55441 tgttgaacgg gccgaaaaaa cacgccaaag gctacatcga aggtctggaa atgctggctt
    55501 caatgcgcct gtgtgctaac gttcctgcgc aacacgccat tcagaccgcg ctgggtggtt
    55561 atcagagcat cagtgaattt attacccctg gcggtcgtct ttatgagcag cgtaaccgcg
    55621 cgtgggaact gatcaacgat attccgggcg tttcctgcgt gaaacctcgt ggtgcgctgt
    55681 atatgttccc gaaaatcgac gccaaacgct ttaacattca tgacgatcag aaaatggtgc
    55741 tggatttcct gttgcaggaa aaagttctgt tggtgcaagg gacggcattc aactggccgt
    55801 ggccggatca cttccgcatt gtcacgctac cgcgtgtcga tgatatcgag ctgtctttga
    55861 gcaagttcgc gcgtttcctt tctggttatc atcagctgta atcttaattt cactgccgga
    55921 gattgcatcc ggcagtgtta tcccgccaca atgacctgat gatgtcatca tacgtaaggt
    55981 cactatgaaa cagagccatt tttttgccca tctctcccgc ctgaaactca ttaaccgctg
    56041 gccgctaatg cgcaacgtgc ggacggaaaa tgtgtccgaa cacagtttgc aggtagcgat
    56101 ggtcgcccat gcgctggcag ctatcaaaaa tcggaaattt ggcggtaatg tcaacgccga
    56161 acgtatcgct ttactggcga tgtaccacga tgcctcagaa gtgctcaccg gcgatctccc
    56221 tactccggtg aaatacttca atgcgcaaat cgctcaggag tacaaggcta ttgaaaaaat
    56281 cgctcagcag aaactggtcg atatggttcc ggaagaactg cgggatatct ttgcgccgtt
    56341 aattgacgag catgcatata gcgatgaaga aaaatcgctg gtgaaacagg cagatgcact
    56401 gtgtgcatat ctgaaatgtc tggaagaact cgcggccgga aataatgaat tcttgctggc
    56461 aaaaacgcga ctggaagcga cgcttgaagc gcgtcgcagc caggagatgg actacttcat
    56521 ggaagtattt gttcccagct tccatctttc gctcgatgag attagccagg attcaccgct
    56581 gtaagcagcc ggagtctgcg tcgcatcagg caataagcgc cggatgcgac atcaggctct
    56641 tgtcaaaacg gaaacagcat cgggatcatc accacacaaa ccgccatcac gataatggtg
    56701 aacggtaccc ccaacttcac aaagtcactg aagctgtaat ttcccggacc taaaaccagg
    56761 gtgttaacag gtgaagacac cggtgtcata aaggcggcgg atgctgccat cgccacgacc
    56821 atcgcaaatg gataaggcga cactcccatc gttttggcag cagccagcgc aatcggagcc
    56881 atcaacaccg ccgtcgcggt attagagata aacagcccaa taaccgccga caagacaaac
    56941 aaacacccca gcatcatatg tggcccataa ccgccgccaa tatccatcag ccctttcacc
    57001 gccagcgcga cacctcccgt tttctgtaat gccacagcaa acggcatcat cccaacgatc
    57061 aaaataatgc tcggccagtg aatggattta taggcgcttt cagcatctat acagcggaat
    57121 ttccccatca gcaggcaggc gatgatagcg gcaacaggat taggaatttc atttgtcagc
    57181 attaacgcca ccattagcac cagacagaaa atggcatggg gtgcctggct gtgcgcgggc
    57241 gatgcttcac tcacctcttc cggtaagttc agcgctacga agtcgcggcc ctgtttggcc
    57301 agcataccga tcagtttcca gttacccaca accaggatga tatcgcccag cagcagaggc
    57361 tcatccgcca gcgaaccttc cagcgccacg ccattgcgct tgagccccac cacattcagt
    57421 ccgtagcggg tacgaaaacc aatttcgcgc accgatttac caatcagttc tgactcagga
    57481 attaatgaaa tctctgccat gcccacatca agggcctggt cagaaaaata ctcgccgcgc
    57541 agtaccatcg gctccagcaa ttgctcacta caaaattgcc ggagatcgac atcagccgca
    57601 gacatatcaa taagcaaaac gtcacgcgcg cgaaattcag aaaccccatt aacgttcacg
    57661 ataacgcgac gaaaacgccg ccagcgttca acaccgatga cgttagcgcc ataacgctca
    57721 cgtaatttga gatcatccag ccgttgacct atcattggcg atccggggcg aatagccaga
    57781 cgtcgcgcac gcccggtcag tcgatattca cggataagat cgcgaaaggt tcgacgcgtc
    57841 cagccttcgc gctgcggggt ctgggtatcc cctttcagca tgaaacgcat cactaacata
    57901 tacaaaatac ccagcaccag cacgaccagg ccaataggtg ttacgctaaa gaaactgaag
    57961 ccgtgatagc cttcacgcag caattcactg ttgactacca ggttcggcgg cgtcgccacc
    58021 agcgtcatca tgccgctaat cagcccggca aaacttaacg gcatcatcag acgtgacggc
    58081 gaagtttgca tacgcatggc aacgcttaac accacgggaa taaagatagc gacaacgctg
    58141 gttgaactca taaacgcgcc aagccccgcg acggtcagca tcaacaaaac caacattttg
    58201 atttcactgt tgcccgccac tttgaccagc catgttccca ttacggtggc aacaccggta
    58261 cggaccaaac catcgccaat aataaacaag gcggcaatca ggacaacgtt aggatcagaa
    58321 aagccggaaa atacttcggg gaccgtcagc gttccgctta atgcaaacgc gacaataaca
    58381 aacaaagcga ccgcatccat acgcactctg cccgtcgcaa acaagacgat ggcaacggcc
    58441 agtaatgaaa gaacccaaat caattcaccg ttcacaactt atccttgtta attgaggggg
    58501 atgacttgat tctgccataa aaaagccccg acgagacggg gctaaatcat gatcaggtgt
    58561 ttcactgaat aataacatcg ccatttggct gtttggtcac agtaatttgc tccagactgt
    58621 ggaggaccaa atcgacctca ttcaggcgcg gggtatctgc cggagcgtta accgcaatga
    58681 catggcaacc cgccgctagg ccagaaagca cgccagcggg agcatcttcc accaccacac
    58741 actcctgcgg cgcaagcccc agcagctgcg cacctaacag atacgcatca ggttctggtt
    58801 ttccgcgctt cactcgctca gcggttacaa acacctctgg tgcgggaagc cccgctattt
    58861 tatggcgcgc tcgcgctacc ggcatggagc cagaagtcac aatggcccac ggaatacctg
    58921 ctttattcaa atgattgagt aaggcgattg ccccagaaag cgcggtaata ccttcggttt
    58981 ccgtggcctc gatttgctcc agacgcgtaa actcggcggc aatatcagcc tcggatttgc
    59041 ccgccataaa atggcgcaaa gaggtgatcg cctgtttacc gtgaatgaaa gccagcacct
    59101 cttccggcgc taacccatga cgtctggccc agttgctcca cgcccgttct accgcaggca
    59161 gggaatccac cagcgttcca tcaagatcaa acagaaaacc tttgcaccgc acgcgggcct
    59221 cctcaggcgt tgataatttg attaatttcg ttggcgctca aatggtactg acgcgggcag
    59281 gcatgccaca cattaagcat gcgctgatat ttttcccaca ttggcgtctg ggcgttaaaa
    59341 ccatgagtcc cagcgtcaaa atgggtatag cgcccttcca cattaaccat aaagcggaca
    59401 taaccgaggt aacgtgcttc agtggcggcg tcaaagccga ggaaggtgac acgacgttca
    59461 tcgatggatt gctggtcctg caaattagac caggaaacat gcaacgcatg atacatctcc
    59521 ataatgtcga tgatagtgcg gcaggtttct tctttcagct cgccaaactc gcgatccaat
    59581 tcacgcatct gtaatccgta accacgctca ataattgttt gcaggcgacg gtaacgttca
    59641 gcatttgccg gatcgagcat agtcatcatc ttgtactgat tagacaaaat aagacgttgc
    59701 gcgttggtca tttccattgt tgactcctgt atcactctac tacggtgaaa aaaaagaagg
    59761 ctgagtatgc cttcttttat atgcgtaatc aggggtcaat tacaaatcat caaggaaagt
    59821 tttatccagt tgtttgaagg cgcgcttaag cgtgtcagct aatgcctggt aatcaggctt
    59881 gccttcaacg ggtgccaaca cctgtccaga ctcctgcaat ttaccgcgaa cttcataaaa
    59941 ccagttaagg attgcagggg gtaatggcgt tacagaacgc ttgcccagcc accacaatcc
    60001 ctgcatgggt aaacttaagg cgaacagtgc agtggcaact gccggcccaa gcagaccgcc
    60061 cagggcaatc tgccagcaga gagtaaatac ggcgatcggc ggcataaaac ggatcgcata
    60121 acgcgtcatc ttgataacgc gattttcgac aaagaccggg gcaaggcgtt tttccagtgg
    60181 ccacgtcttt gagtaatgct gtccgcggcg aaacaagcta aaaaaattaa cagaacgatt
    60241 atccggcgtt gacatgcttc acctcaactt cacatataaa gattcaaaaa tttgtgcaaa
    60301 ttcacaactc agcgggacaa cgttcaaaac attttgtctt ccatacccac tatcaggtat
    60361 cctttagcag cctgaaggcc taaatagtac atattcattg agtcgtcaaa ttcatataca
    60421 ttatgccatt ggctgaaaat tacgcaaaat ggcatagact caagatattt cttccatcat
    60481 gcaaaaaaaa tttgcagtgc atgatgttaa tcataaatgt cggtgtcatc atgcgctacg
    60541 ctctatggct ccctgacgtt tttttagcca cgtatcaatt ataggtactt ccatgtcgag
    60601 taagttagta ctggttctga actgcggtag ttcttcactg aaatttgcca tcatcgatgc
    60661 agtaaatggt gaagagtacc tttctggttt agccgaatgt ttccacctgc ccgaagcacg
    60721 tatcaaatgg aaaatggacg gcaataaaca ggaagcggct ttaggtgcag gcgccgctca
    60781 cagcgaagcg ctcaacttta tcgttaatac tattctggca caaaaaccag aactgtctgc
    60841 gcagctgact gctatcggtc accgtatcgt acacggcggc gaaaagtata ccagctccgt
    60901 agtgatcgat gagtctgtta ttcagggtat caaagatgca gcttcttttg caccgctgca
    60961 caacccggct cacctgatcg gtatcgaaga agctctgaaa tctttcccac agctgaaaga
    61021 caaaaacgtt gctgtatttg acaccgcgtt ccaccagact atgccggaag agtcttacct
    61081 ctacgccctg ccgtacaacc tgtacaaaga gcacggcatc cgtcgttacg gcgcgcacgg
    61141 caccagccac ttctatgtaa cccaggaagc ggcaaaaatg ctgaacaaac cggtagaaga
    61201 actgaacatc atcacctgcc acctgggcaa cggtggttcc gtttctgcta tccgcaacgg
    61261 taaatgcgtt gacacctcta tgggtctgac cccgctggaa ggtctggtca tgggtacccg
    61321 ttctggtgat atcgatccgg cgatcatctt ccacctgcac gacaccctgg gcatgagcgt
    61381 tgacgcaatc aacaaactgc tgaccaaaga gtctggcctg ctgggtctga ccgaagtgac
    61441 cagcgactgc cgctatgttg aagacaacta cgcgacgaaa gaagacgcga agcgcgcaat
    61501 ggacgtttac tgccaccgcc tggcgaaata catcggtgcc tacactgcgc tgatggatgg
    61561 tcgtctggac gctgttgtat tcactggtgg tatcggtgaa aatgccgcga tggttcgtga
    61621 actgtctctg ggcaaactgg gcgtgctggg ctttgaagtt gatcatgaac gcaacctggc
    61681 tgcacgtttc ggcaaatctg gtttcatcaa caaagaaggt acccgtcctg cggtggttat
    61741 cccaaccaac gaagaactgg ttatcgcgca agacgcgagc cgcctgactg cctgatttca
    61801 caccgccagc tcagctggcg gtgctgtttt gtaacccgcc aaatcggcgg taacgaaaga
    61861 ggataaaccg tgtcccgtat tattatgctg atccctaccg gaaccagcgt cggtctgacc
    61921 agcgtcagcc ttggcgtgat ccgtgcaatg gaacgcaaag gcgttcgtct gagcgttttc
    61981 aaacctatcg ctcagccgcg taccggtggc gatgcgcccg atcagactac gactatcgtg
    62041 cgtgcgaact cttccaccac gacggccgct gaaccgctga aaatgagcta cgttgaaggt
    62101 ctgctttcca gcaatcagaa agatgtgctg atggaagaga tcgtcgcaaa ctaccacgct
    62161 aacaccaaag acgctgaagt cgttctggtt gaaggtctgg tcccgacacg taagcaccag
    62221 tttgcccagt ctctgaacta cgaaatcgct aaaacgctga atgcggaaat cgtcttcgtt
    62281 atgtctcagg gcactgacac cccggaacag ctgaaagagc gtatcgaact gacccgcaac
    62341 agcttcggtg gtgccaaaaa caccaacatc accggcgtta tcgttaacaa actgaacgca
    62401 ccggttgatg aacagggtcg tactcgcccg gatctgtccg agattttcga cgactcttcc
    62461 aaagctaaag taaacaatgt tgatccggcg aagctgcaag aatccagccc gctgccggtt
    62521 ctcggcgctg tgccgtggag ctttgacctg atcgcgactc gtgcgatcga tatggctcgc
    62581 cacctgaatg cgaccatcat caacgaaggc gacatcaata ctcgccgcgt taaatccgtc
    62641 actttctgcg cacgcagcat tccgcacatg ctggagcact tccgtgccgg ttctctgctg
    62701 gtgacttccg cagaccgtcc tgacgtgctg gtggccgctt gcctggcagc catgaacggc
    62761 gtagaaatcg gtgccctgct gctgactggc ggctacgaaa tggacgcgcg catttctaaa
    62821 ctgtgcgaac gtgctttcgc taccggcctg ccggtattta tggtgaacac caacacctgg
    62881 cagacctctc tgagcctgca gagcttcaac ctggaagttc cggttgacga tcacgaacgt
    62941 atcgagaaag ttcaggaata cgttgctaac tacatcaacg ctgactggat cgaatctctg
    63001 actgccactt ctgagcgcag ccgtcgtctg tctccgccag cgttccgtta tcagctgact
    63061 gaactggcgc gcaaagcggg caaacgtatc gttctgccgg aaggtgacga accgcgtacc
    63121 gttaaagcag ccgctatctg tgctgaacgt ggtatcgcaa cttgcgtact gctgggtaat
    63181 ccggcagaga tcaaccgtgt tgcagcgtct cagggtgtag aactaggtgc aggcattgaa
    63241 atcgttgatc cagaagtggt tcgcgaaagc tatgttggtc gtctggtcga gctgcgtaag
    63301 aacaaaggca tgaccgaaac cgttgcccgc gaacagctgg aagacaacgt ggtgctcggt
    63361 acgctgatgc tggaacagga tgaagttgat ggtctggttt ccggtgctgt tcacactacc
    63421 gcaaacacca tccgtccgcc gctgcagctg atcaaaactg caccgggcag ctccctggta
    63481 tcttccgtgt tcttcatgct gctgccggaa caggtttacg tttacggtga ctgtgcgatc
    63541 aacccggatc cgaccgctga acagctggca gaaatcgcga ttcagtccgc tgattccgct
    63601 gcggccttcg gtatcgaacc gcgcgttgct atgctctcct actccaccgg tacttctggt
    63661 gcaggtagcg acgtagaaaa agttcgcgaa gcaactcgtc tggcgcagga aaaacgtcct
    63721 gacctgatga tcgacggtcc gctgcagtac gacgctgcgg taatggctga cgttgcgaaa
    63781 tccaaagcgc cgaactctcc ggttgcaggt cgcgctaccg tgttcatctt cccggatctg
    63841 aacaccggta acaccaccta caaagcggta cagcgttctg ccgacctgat ctccatcggg
    63901 ccgatgctgc agggtatgcg caaaccggtt aacgacctgt cccgtggcgc actggttgac
    63961 gatatcgtct acaccatcgc gctgactgcg attcagtctg cacagcagca gtaatctcgt
    64021 catcatccac agctttgcgc tgcggatatc tgaaccggaa ataatcacta tttccggttt
    64081 tttattctct taatttgcat taatcctttc tgattatctt gcttaactgc gctgcatcaa
    64141 tgaattgcgc catctcactt tgcatactta ccactttgtt ttgtgcaagg gaatatttgc
    64201 gctatgtccg caatcactga atccaaacca acaagaagat gggcaatgcc cgatacgttg
    64261 gtgattatct tttttgttgc tattttaacc agtcttgcca cctgggtagt tccggtgggg
    64321 atgtttgaca gtcaggaagt gcagtatcag gttgatggtc aaacaaaaac acgcaaagtc
    64381 gtagatccac actcatttcg cattctgact aacgaagcag gcgaacctga gtatcaccgc
    64441 gtacagctgt tcacgacggg cgatgaacgc ccgggcctga tgaacttccc gtttgaaggg
    64501 ttaacctcag gatcgaaata cgggacagcc gttggcatca tcatgtttat gctggtgatt
    64561 ggcggcgcgt ttggcattgt gatgcgtaca ggaaccattg ataacggtat cctggcgctt
    64621 attcgccata cccgcgggaa tgaaattctc tttattcctg cgctgtttat tctgttttca
    64681 cttggcggcg cggtatttgg tatgggagaa gaggccgtcg cctttgccat tatcatcgca
    64741 ccgctaatgg tccggctggg ctatgacagt attaccaccg tcctggtgac ttatattgcc
    64801 acgcaaatcg gttttgccag ttcgtggatg aacccgtttt gtgtggttgt tgctcagggg
    64861 attgccggcg ttccggtgct ttctggctcc gggttgcgca tcgtggtgtg ggttatcgcc
    64921 actctgattg gcctgatctt taccatggtg tacgcctcac gagtgaaaaa gaatcctctt
    64981 ctgtcacgcg tgcatgagtc cgaccgcttc tttcgtgaaa agcaggcgga tgttgaacaa
    65041 cgtccgttta cctttggtga ctggctggta ttgattgtcc tgacagccgt aatggtctgg
    65101 gtgatttggg gcgtgatcgt taatgcctgg tttattccag aaattgccag ccagttcttc
    65161 accatgggtc tggtgattgg catcatcggc gttgttttcc gccttaacgg catgacggtt
    65221 aataccatgg cttcatcctt taccgaaggg gcgcgaatga tgatcgcccc tgccctgctg
    65281 gtgggtttcg ccaaagggat tttgctgctg gttggtaatg gtgaagcagg taatgccagc
    65341 gtgttaaata ctatcctcaa cagcattgcc aatgccatta gcggtctgga caacgcggtc
    65401 gcggcctggt ttatgttgct cttccaggct gtatttaatt tcttcgtgac gtccggttct
    65461 ggtcaggcgg cattaactat gccgttactg gcaccgcttg gcgatctggt cggtgttaac
    65521 cgtcaggtta ccgtgctggc gttccagttt ggtgatggct tcagccacat catttaccca
    65581 acctcagctt cgttaatggc aacgctcggt gtgtgccggg tggacttccg taactggctg
    65641 aaggtgggcg cgaccctgct tggactgctg tttattatgt ccagcgtcgt agtgatcggc
    65701 gctcagttga tgggctacca ctaaaaatgt taagagccgc aattgcggct cttttttcat
    65761 tctgccgttt cagtctctac ggcttcattt ttggcattgc gcttcatcca caacgccagc
    65821 gctttcagcg agtctggagt gaactcatcg cagcgtgcgg tgatttcttc cggcgtcagc
    65881 cagcaaactt cactgacttc atcttcctgt agggcgaagg gaccgtgaga gacgcaactg
    65941 aacaacgcgc cccagacacg gcaattttta tcttcgaaat agaactgccc gtgctcggca
    66001 aaggggacac cggcaatgcc caactcttct tccgcttcgc gacgcgcgga ttccagcagt
    66061 tgctcatcgg cctgaactac accgcctgcg gtcgcatcta acatgccggg taaaaagtct
    66121 tttgtctcgg tacgacgctg gaccagaatt ttgcccatgc catcatgcac gacgatgtaa
    66181 gttgcacgat gacgcagaca ctgtgcccgc atttgttccc ggctggcttg tgcaatgact
    66241 tcgttctctt cattgacaat atccacccat tcagtacttg ccaaacgacg ctgttccatt
    66301 atcaggaaac cttctttttc tggcgctctt acggcgcatt ttgagttgtg ggtaaattac
    66361 ggattaatcg cgacctgcgc aatgatactt tgatcattga gtgcgattac actaagtacg
    66421 tcattatcca gcatgccata actcgccgga ttaccgcctt tcggaatact caccgagccg
    66481 gggttgaagt ggaaaatctc cccccgctgt tctgccactg gcagatgggt atgaccgtac
    66541 accagcacat cgttctggtt taaagcaggt agattttccg ggccaaaaag atgaccatgc
    66601 gtcaaaaaca gacgttgttt ttccagtaat acctgttgcc acggcgcggt tatcgggaaa
    66661 tgcagcatca tttgatccac ttcgctgtcg cagttgccac gcacagcgat aaccttatgt
    66721 gccacttcat taagccgttc agcgactttg gctggcgcgt aaccctccgg taaagcatta
    66781 cgcgggccgt gattcaacac gtcgccaagg atcaccagcc actgggcacc gctttggaca
    66841 aacaactcca caacacgttc cgtcgccggt aacgacccat gaatgtccga tgcaaacatc
    66901 agtttcatca ctcactcctc gtcgaaaaaa cgtaagctat gatactggat tctgtcgttg
    66961 ctatcagcca gattgcttcg ccgaaagtgc aataaaacgc tggacagacg ctcgctgcca
    67021 ctggaatgtc gcataatcca ccaggcgttc tggcacctca tcgccatgta gtaccaggcg
    67081 gttaatcatt agcgccagat cagtatcagc aatgcaccat tcaccaaata aattcggctg
    67141 acccagtgct aacaaatgtt ctgccatcgc gaacagtttc tctgcactgg cttttccctc
    67201 ggccgttagt ggcgcttttt tcgcccccgc aaagacaaca tccgtcggac gctcttcgcg
    67261 gatgggcatc agatcgctgc gcagccaggc ctgaatctgt cgtgcacgcg cacgattttc
    67321 taaatcaagc ggataaatac gttcccaggt cggtggcgca aatcgatctt ccagatactc
    67381 cgcaatggca gaagattcac tcaactcaaa atcatcgatt tgtaataacg gcacacggcg
    67441 tgtctgaccg taaccttgcc acgtcggctg caaatgttca ccgctgtcga ggtcgatggt
    67501 cttgatatga aacgacaggc ctttttcctg caacgccacc caggcggata acacataagg
    67561 ggagaaaaag tgggcatctg accaaagcgt gatagcgggt ttactcatta tgtcctcggc
    67621 aataaagggg gctggctatc aaactataga gcctttttgt cactgtcacg tgacgaatac
    67681 tcacgtaagt cgccgcgctt tctatacttg tggtgttcag atgattaaac aaccggagct
    67741 gcaatgatcg atctctattt cgccccgaca cccaatggtc acaaaattac gctgtttctc
    67801 gaagaagcag agcttgatta tcgcttgatt aaggtagacc tggggaaagg cggtcagttt
    67861 cgtccggaat ttttgcgcat ttcgcccaac aacaaaattc cggcaattgt tgatcattct
    67921 cctgccgatg gcggcgaacc gttaagcctc tttgagtctg gtgccatttt gttgtatctg
    67981 gctgagaaaa caggactctt tttgagtcat gaaacgcgtg agcgcgccgc cacattacag
    68041 tggttattct ggcaggtagg cggactgggg ccgatgcttg ggcaaaatca tcattttaat
    68101 cacgcagccc cccaaaccat tccttacgct attgaacgtt atcaggttga aactcagcgt
    68161 ctttaccatg tactaaacaa gcggctggaa aactcgccct ggctgggagg cgagaactac
    68221 agcattgcgg atattgcctg ctggccgtgg gttaatgcct ggactcgcct gcgaattgac
    68281 ctcgcaatgt atccggcagt caagaactgg catgagcgga tccgttcgcg ccctgccacc
    68341 gggcaggcac tgctaaaagc acaactcggt gatgagcgtt cggatagtta acagaaacag
    68401 gttctcgtgt attatttcat cctaagtaaa acaacggaga acctgcaatg gcacaacctg
    68461 ccgctattat tcgtataaag aaccttcgtt tgcgtacgtt tatcggaatt aaggaagaag
    68521 aaattaacaa ccgtcaggat attgttatca atgtgacgat ccactacccc gccgataaag
    68581 cgcgcactag cgaagatatc aacgatgcgc tgaattatcg caccgtaacg aaaaacatta
    68641 ttcagcatgt agagaataac cgtttctctt tgctggaaaa attaactcag gatgtgctcg
    68701 atatcgcacg tgaacatcac tgggtgacgt atgctgaagt ggagatcgat aaactgcacg
    68761 cgctgcgcta cgccgattcg gtatccatga ccttaagctg gcagcgttaa tcgccatatc
    68821 gggaggctgc atgaacatag tgatcaccgg agggacggga ttaattggtc gccatttgat
    68881 tccgcgtttg ctggagctgg gccatcaaat tacggtagtg acgcgtaacc cgcagaaagc
    68941 cagttccgtt ctcggccctc gggtgacact atggcaaggg cttgccgatc aaagcaacct
    69001 caacggcgtt gatgcggtaa tcaacctggc cggagaaccg attgctgata aacgctggac
    69061 tcacaagcaa aaagagcgtc tctgccaaag ccactggaat atcacgcaaa aactggtcga
    69121 tttgattaat gccagcgaca cgccgccgtc ggtactcatt tccggctcgg caacgggcta
    69181 ttatggcgac ttaggtgaag tggtggttac cgaagaggaa ccgccgcata acgaatttac
    69241 tcataaactc tgcgcccgct gggaagagat tgcctgccgg gcgcaaagtg acaaaacgcg
    69301 agtgtgcctg ctgcgaaccg gcgtggtact ggcaccggac ggcggtattc tcggtaaaat
    69361 gctgccaccg tttcgtcttg gcctgggcgg gccgattggt tccggtcggc agtatctggc
    69421 ctggattcat attgatgata tggtcaacgg cattctctgg ctgctggata acgagctgcg
    69481 cgggccattt aatatggttt cgccctaccc ggtacgcaat gaacaatttg cccatgcgct
    69541 cggtcatgcg ctgcatcgcc cggctatttt gcgcgtcccc acaaccgcca ttcggctgtt
    69601 aatgggcgaa tcttcagtac tggtattagg tggacaacgc gcgctgccta aacggctgga
    69661 agaagcgggt tttgcgtttc gctggtacga tttagaagag gcgctggcgg atgtcgttcg
    69721 ctgatgtggt ttacagcaaa catccgccag ttaactccct gtgttacagg attagtggct
    69781 ttgcgcgata agatcgtctg gtgaaagtcg ggtcaccatc ataactaact ctctgtctaa
    69841 acctctatcc agcatctcct gagcaatacg cagggcttct tcgtgtttgc cctgcattgc
    69901 gccttcttca cgtaatctgt cagcaatggt catcagtttc tccttttctt gtggtgcgcg
    69961 ttctgttatc tcaccaataa atgcacggaa acgctgggca tcccctgttt gtaatacgta
    70021 attaaacagg gcttttagct gtctgtcatt agtgttccct gtaactagca gcgaaacaat
    70081 ttggtcgact aatcccaaca gatcgcgctg acgaatatgt ttctgaatta actccaacag
    70141 cgccattttg cggtgttgca taatctcgtc atccggcacc acggcaatat ccaccaacgg
    70201 aaacgctgat gaatatattt tgcgggctat agcaggctcg gcaaattcat caagccagca
    70261 gagtgaataa ggataaggac ttctgcaacc atgataaaac agcatcggga tcaccaatgg
    70321 aagctcttta tagcccgcat caagatggtt ttgcattgcc gcaatggaat aacgcatcat
    70381 gcgaaaagcc attaattctt ccggcttact ttggtgctct atcactacat aaatataacc
    70441 cactccctcc tgcgttttca cagaccacaa gaggtcggaa taatattgcc gcaggtcttc
    70501 atcaataaaa ctgtttggtt ctagtttaag cgtcgttaaa tcacacagtt tgcgcagcgg
    70561 cgcgggaaga tgaatattaa taaaattccg cgcggtgtct ggatggcgta aaaaagattt
    70621 aaataccgca tcatgcggcg tggaagttgt cgatattgtc atggcgttcc gtcacccctc
    70681 aaatttaaga tgacgcgaca ataaccacga ttaacaccca atgcagcagg cagtttttat
    70741 ctttggacag cgaattcaga agtattttta gcggactgca acttttacat ttcagcggaa
    70801 gctgactcgc aaattcccct tgaaaatact ccggcagcgt tagccaccgg agaggaaatt
    70861 atttcagcga tcccttaagg aactgttgca gacgagggct ttgcgggttg ccaaataact
    70921 gctccggcgc gccctcttct tctattttcc cctgatggag gaaaatgacg tgagtagaaa
    70981 catggcgagc aaagcccatt tcgtgagtca ctaccaccat ggttttcccc tcttctgcca
    71041 gttgctgcat aatacgtaac acttcgccta ccagttcagg atcgagcgcc gaggtaggtt
    71101 catcaaacag taaaacttct ggttccatcg ccagcgcccg cgcgatagaa acacgctgtt
    71161 gctgaccgcc ggaaagatgc accggatatt tcccctgcgc acgttcgtct atcccgactt
    71221 tcgccagata cttcaccgcc cgctcgcacg cttcctgctt gctcaggccc aacacctgaa
    71281 tcggcgcttc catgacgttt tccaacaccg tcatatggct ccagagattg aagtgctgga
    71341 ataccatcgt caggcgtgtg cgcagtaagc gcagttgatt tttatcggcg actttgagtt
    71401 gaccgtcttt gtcgcgcacc agattgatcg tctggccatt gaccacgatc gatccttcac
    71461 tcggtttttc gaggaagtta atgcagcgca gaaaggtact tttccccgat cccgacgatc
    71521 cgatgatgct tattacatct ccggcattcg cttgcagtga gacccctttc agcacttcgt
    71581 gttcgccata gcgtttgtgc aaatcgataa cgtttaattt attctcggac atcgtattct
    71641 cagtgcgttg aagaaggttt cacatgctgc aaccagcgtt tttccgctct gcgaaagagg
    71701 ctgatcagga cataagagat gattaaatag agcaccgcgg caatgccgaa ggcggtaaaa
    71761 ggttgatacg tggcggcgtt aatatcgcgg gctattttca gcagatccgg cacagtggcc
    71821 gtaaacgcca gcgcagtaga gtgcagcatc aggatcactt cgttgctgta tgccggtaac
    71881 gcaatacgca gcgcagaagg caaaataatg cagcgataca ttttaaaggt cgagaagcca
    71941 taggctctgg cggcttcaat ttccccatgc ggaaccgaac ggattgcccc agcaaaaatc
    72001 tcggtagtgt aagcgcaggt gttaagcgtc agcgccagca cggtacagtt caggccactg
    72061 cggaagaaag cgttaaggaa ttcggttccc ttaacaatct caagcgtgta catgccggaa
    72121 tagaacacca gcaactgaac atacagcggc gtaccgcgaa aaatataggt aaataaccag
    72181 attggaaact ggatgtattt attactggag acacgaccaa tcgccagaaa cagcgccagg
    72241 actccgccta tcactaccga caaaataagc agccacagag tgatcgccac accagtaaag
    72301 cgataaccgt cggtccacag cagcggtttc cagtattcat gtaagatttc gatcacaggt
    72361 cagccctctt cacacccacg gagtagcggc gctcaaggaa cagcagcaca ccattggaaa
    72421 cggtggtgaa aaccaggtaa atcacgccac agacgatggc gaaatagaac ggttcccagg
    72481 tacttttgcc tgccagctgc gtggctttga ccacatcttc caggccgagt aacgaaacca
    72541 gtgcggtaga tttgaggatc acctgccagt tgttgccaat gcctggcagg gcgtaacgca
    72601 tcatcgccgg aaacatgatc ctccgaaaca cttgcccgcg agtaaaaccg aacgccgtcg
    72661 ccgcctctat atgccctttc ggcactgcca taaacgcgcc acggaacgtt tcggtaaaat
    72721 aagcaccgta gataaaaccg agagtgataa taccagcgac catcggatcg atatcaatct
    72781 gcccgacgcc catcgcctcc gtcaccatgt ttagcgcaat ctgcaaacca tagaaaatca
    72841 gcagcatcaa caccaagtcc ggcacgcctc gaatcagcgt ggtgtaccct tcgaaaatca
    72901 gccccaaaag ccgattttgc gagagcttac cgccagcgcc aattaaaccg atgattacag
    72961 cgagcactac agagctgata gccagctcca gcgtgacgag cgcaccctgt aaaataacac
    73021 ctgaaaaccc atacaacatg ctgcctgtcc tgtcgtgtgt ggtgaattac cgcctcttgt
    73081 ctccctccgg ttacccgaag ggagcgggct ttctttcacc gatggcgatt aaccaccata
    73141 aacatcaaaa tcgaagtact ttttcgctaa tttctcgtaa gtaccgtcag cgcgcatttc
    73201 ggcaaaggct ttgttcagtg cctcgcgcag ttcgttatct tctttacgca ggcccatgcc
    73261 ggtccctacg ccaaacagtt tttcatcttt aacagacgga ccaccgaatt tgtaatcttt
    73321 accgacgggt tgtttgagga aaccttcgct ggcagcgacc tcatcctgga acgcggcatc
    73381 aatacgtccg gcagtcaggt cagaataaat gttgtcctgc ccctgatacg agacgatttc
    73441 aatgcctttt ggtgcccagt gttcattacc gaacgtctcc tgggtggtgc cctgcaatac
    73501 gcctacccgt ttgcctttca gcgactcgac tgtcggctga atgtcagaat ttttcgccac
    73561 caccagacga gaatcggcag cgtacagttt gtcggtgaag gcaatttctt gctgacgttt
    73621 ttccgtaatg gaaagcgatg acatgatggc gtcaatcttc ttcgctttta aggacgggat
    73681 taacgcatcc agcgggtttt cgacaaacgt acattgcgta ttgatgcgct tgcacaactc
    73741 cttcgccagc tcgatatcga aaccaaccag ttcgccttgt gaattttttg attcaaatgg
    73801 cgcataggtc gggtcggtac cgatgcggat gttttgcgga attgcagcaa gtgccgcagt
    73861 tgcgctggag aaggccagaa ccagagagag cgatagcacc agttttttca taatgtcctc
    73921 aactgacagt ctttttatag ggaatattta caggtttgtt acacttatcg tgccataaaa
    73981 ttgaccatca aggcaaaata tcccgccccg ttagacgtat tatttttcat taaaaatgct
    74041 taagtatgca tttcaatgca ccacgatagt gcaacaaacc tggcgtgcac catgatgatc
    74101 attccatcag gtacagcttc ccagcgacgt atcagtcacc gtagacatta aagtcgaaat
    74161 actttttcgc catcttgtcg taggtgccgt cctgacgcag ctcgccaagc gccttattga
    74221 aggcagccgt cagttcagca tcatctttac gtagccctac accggtgcca tcaccgaagt
    74281 attttttgtc ttttactgat gagccagcaa aggcgaaatc tttaccagca ggttgcttga
    74341 ggaatccttc gctggcagca acttcatctt gtaacgcagc atccagacgt cctgcagcca
    74401 gatcggaata gaccaaatcc tggttggcat aggccaccac atccacgcct ttactacgcc
    74461 aggtctcgtt agcgtaagct tcctgggttg atccctgcag cacaccaaca tgtttacctt
    74521 tcagtgaatc cagcgttggc tgaatcggtg aacctttggc agcaatcaaa cgagaatctg
    74581 cggcgtacag cttgtcggag aaggcaatct cctgctgacg tttatcggta atggaaagcg
    74641 acgaaataat agcgtcgatt tttttcgctt tcagtgaggg gagcagcgcg tcaaagtcac
    74701 tggcaaccca ggtacatttc acctgcatcc gtttgcacat ctcgttaccg agatcgatat
    74761 caaagccaac aaaatcacct ttagcatctt tcgacgagaa cggcgcatag gtggtatcgg
    74821 ttccgatacg taccgtctcc ggtagcgccg catagctgga agccgctgcg gagagaccga
    74881 ctaataaaga cagagcgaga atcgacttct tcatacataa ccctcaagtg aaaatggctt
    74941 ttttatgttg tgtattgtgt tgtgtgtttg caggctcttt catgcaggtc ttatgccatc
    75001 ttgacgacaa cagtaacatt caacgttaaa tatgttaata agacgttgca ttattgtcct
    75061 gaagttgaag atagcaggta tagcggttga atcgcagcgt ttcgattgaa tggcagaaac
    75121 aaaatgtcga ggatttgatc gcggttgcaa aattgccctg aaacagggca acagcggagt
    75181 tatgcgccct gccagcgggc aaagagatct tcaggaaggg ttattgcaaa ctggtcaaga
    75241 acacgattaa ccgtctgatt tatcacatca tcaagggatt gcgggcgatg ataaaacgcc
    75301 ggaacgggag gcataatcac cgcaccgatt tctgccgcct gagtcattaa acgcagatgg
    75361 cctaagtgca atggtgtttc acgcacgcag agcaccaacg ggcgacgctc tttcagcacc
    75421 acatctgccg cacgggtcag taagccatca gtatagctat ggacaatgcc ggacagggtt
    75481 ttgattgaac agggtaaaat caccatcccc agcgtctgga aagaaccgga agagatgctg
    75541 gcggaaatat cgcgcgcatc gagcgtgaca tcagctaatg cctgcacttc gcgcagggaa
    75601 aaatccgttt cgagggataa ggtctggcgc gccgcctggc tcatcaccag atgcgtttcg
    75661 atatctgtga catcgcgcag aacctgtaat aagcgcacgc cataaatcgc gccgctggca
    75721 ccgctgatgc ctacaatgag tcgtttcata aatagttgcc ctgtcagact tgcaggcaga
    75781 ctttgcagta tttcggcggg agttgcaagt cagggtgcca gaccggcacc ctcagcgaag
    75841 gcatcatcct tcgttatgca tttcgagatt ttccacttcg ttctgacgtt gcactgcttt
    75901 ggcgtcatca ttacgtaacg tatcgaggaa atcgaggtag ccctgatcaa catctttggt
    75961 gacgtagacg ccgttgaaca ccgagcattc aaactgctgg atatccggat tttcagcgcg
    76021 aacggcgtcg atcagatcgt tcagatcctg gaaaatcaac ccgtcagcac cgatgatctg
    76081 gcgaatttca tcaacttcgc gaccgtgagc gatcagttcc gtggcgctcg gcatatcaat
    76141 accataaacg ttcgggaagc gaatttccgg tgccgcagaa gcgaggtaca ctttcttcgc
    76201 tccggcttcg cgtgccatct cgataatctg ctcagaagtg gtgccacgga cgatggagtc
    76261 gtcgaccagc aggacgtttt tatcgcggaa ctcggcgcgg ttggcattca gtttacggcg
    76321 cacggactta cgacgcagct gctggcccgg catgataaag gtgcggccaa catagcggtt
    76381 tttaacgaag ccctggcggt acggtttgcc cagaatacga gcaatttcca gcgcgatatc
    76441 acacgaggtt tctgggatcg ggatcaccac gtcgatatcc agatcttccc attcgcgggc
    76501 aattttctcg cccagtttcg tgcccatatt cacacgcgcg ctgtaaacgg aaattttgtc
    76561 gataaacgag tccgggcggg caaagtatac atactcaaac aggcacggat tgctgaccgg
    76621 attgtcagca cattgacggg taaacaactg cccttcttca gtgatgtaaa tcgcttcgcc
    76681 cggcgcgacg tcacgcagga aatcaaagcc cagcgtatcg agcgctacgc tttcggaagc
    76741 gaccatatat tctgtacggt tctcgtcaat atcacgtttt cccagtacca gcggacgaat
    76801 cccgtttgga tcgcggaaag caaccatacc gtggccgata atcatcgcca cacaggcata
    76861 cgcgccgcgg attaagcggt ttgtggcagc aatggcagcg aaaatattgt cggcttccag
    76921 cgggtagtgg cggaagttgt ccagctcgct ggcgaagata ttaagcagaa tttccgagtc
    76981 ggaagtggtg ttgatgtggc ggcgtttttc ttcaaacagt tttttacgca actcgtgagc
    77041 gttggtcaga ttgccgttgt gggcaagcgt aatgccatac ggggagttaa cgtaaaacgg
    77101 ctgcgcttca gaggcgctgg agctgccagc cgtagggtaa cgcacatgac caatgcccat
    77161 attgccctgc aaacgctgca tatggcgagc ttcaaataca tcgctcacca gcccgttcgc
    77221 tttacgcaaa cggaagcagt tattggcatc tatggtgatg atgccggcgg catcctgacc
    77281 gcgatgctga agcaccgtta aggcatcata aatcgactgg ttaaccggca taacaccggc
    77341 gataccgaca ataccgcaca tacgtctttt tcctcgttaa gccacatctc agagcactta
    77401 cgctctgggc aagaaacttg acgagctttg cagataatca aaaaaccatc tgatgataaa
    77461 actgaattgc gggatcagct gtgatttgct ccagtcttca cttttcgaca ccccggtaaa
    77521 ggagtcgaga aagaagagaa tggcggcaac aatcaacaca ccgcgcaacg caccgaaaca
    77581 gacgcccagc acccgatcgg tgcctgacaa ccccgttttc tccaccaact ggcctatcac
    77641 gaagttcacg atagcaccaa cgatcagggt agcgataaac agtaccgcga tggcaatccc
    77701 atttcgaacc agttcgtctt caaagcccgt aaaccagact gacaggtaag tgtagtaatg
    77761 actggcaaca aagaaagcac aaccccatgt caccagcgat aacgcttcac gaacaaagcc
    77821 gcggatcagg ctaaccagag aggaaaaagc aatcaccgcg attatggcgt aatcaatcca
    77881 gaccatatgt gtcccacgat tttacgccct gtcatcctgt tcggggcgca ttctaacaga
    77941 aaaagaaaac gtttgcgtag ggatttcctt cccgcgcatc aataaaaatg gcgctgaaaa
    78001 aatattcaac gccatcgact ttttatgcct ttgcggcatc gtgcaatgcg tgtcggatgc
    78061 ggcgtaaacg ccttatccga cctacggttc taacactgcc caagcctgat aagacgcgcc
    78121 agcgtcgcat caggcaagac tgtgttaatt cggcgtatag cccattacca cgccacttaa
    78181 gccagaaagt tgcttcaact cacccagcga acctttcagc ttatctttcg aggcatccgg
    78241 cccaaccaga atacgggtaa ttttaccctg cactggcgtg gatggcgacg tataaacccg
    78301 ataaccggca ccgcgcagct taccgacaat ctcattcact ttatcggcat ttttcagcgc
    78361 acccagttgc acaacatagg ctttacccgt tggcgctgct ttttcttcca cgaccggctt
    78421 cggttctggc ttcggcgcag gtggtgcttc aacctttggt ttaggcggct ccaccggttt
    78481 cggcttcggt ggggcgaccg gtgcaggttc cggttcaaac tcggtgttat tggctgcaat
    78541 agtggccgga tcgagcgacg gtgctgcggc atcacctgcc cgcacctctt ccgctgcgcc
    78601 ttccggcggc tgcgtcggca acgcctgggt ggcggctggc atcatatcag gctcatcacg
    78661 atcgcccgct ttcggcacca gcgggatagc cgcgaactca tcctgataat gttttttctg
    78721 cccgtccagc agccctggaa gtacaatcac ccccagcgcc accagcacga tcgtgcccac
    78781 taaccgattc tgaaacttac ttgccaccgc ttctcctcgc gtcaatcact tccatgacat
    78841 gtgcgaccgt gtggaaagaa ccacacacca gcacggtgtc ttccgcttta gcgtccgcca
    78901 ttgcggcatc ccatgcctgc gcaacgctat caaatgattt gccgttaccc aaatgctcaa
    78961 gcagttgttc tgccgtggca ccgcgcggcc cttccagtgg cgcacaatac cagtcatcaa
    79021 ccacgctttt caaccaggcc agagttccgg caatatcttt atcatgtagc ataccgataa
    79081 ccgccagcac gcgcccgttt ttcggtagcg ctttcatacg cccggtgaga tattccgccg
    79141 catgtggatt atgcgcgaca tcaaaaataa cgcgtggcga ctcgctcaca atctggaaac
    79201 gtcccggcaa aattgcgctg gcaatcccgt cgcgaatggc attttcactg acttccagcc
    79261 cgctggcacg cagtgccgcc agcgccgttg cggcattcgg ttgcgggaca agcggcaacg
    79321 gcagattttc cagcgtgccg tgagcatcgc taaacgccca gtcatgatcg gtgacggaat
    79381 agttccactc aacgccccga cgttgtaaca gtgcaccttt ttcctgcgcc acatcagcaa
    79441 tggtagaagg catttccggc tcaccgacaa ttgccggttt ttcgctgcgg aagatgcctg
    79501 ctttctcgcg accaatactt tcgcgatctg gacccagcca gtcggtatga tccagcgcaa
    79561 tactggttac taccgcggca tcggcatcga caatattggt tgcgtccaga cgaccgccca
    79621 gccctacttc cagaatcacc acgtcaagtt gtgcctgctt gaacagccac aacgccgaca
    79681 gcgtaccgta ctcgaaatag gtcagggaaa tatcaccgcg tgccgattca atctccgcaa
    79741 aagaggcggt gtgggccgat tccggcaatt cccggccctg cacacgtacg cgctcggtat
    79801 aacgcaccag atgaggcgaa ctgtagacgc ccactttgta ccctgccgcc atcagaatcg
    79861 actccagcgt acggcaggtg gtgcctttgc cattcgtacc cgcaacggta aacacaaatg
    79921 gcgctggttt caggacgcca agacgcgccg cgacctggct cacgcgctca aggccgagct
    79981 cgatagtttt actgtgcagg ttttccagat aagaaagcca cgaagccaga ggcgacgcgg
    80041 cttgaggagt gcgtttgata atcatggtat ccgctgattc gttacggtga gaatagcaaa
    80101 agggcagagc cagtggccct gcccttatca gttatcaggc ctcaggttcc tgatccggta
    80161 ccgggggtac cactacgcct tcacgcggcg cttcaggatt cggcgctggc agattcatca
    80221 acttcgccag aatgctcgcc agtttcaggc gcatttccgg acgacggacg atcatgtcga
    80281 tcgcgccttt ctcgatcagg aattcactgc gctggaatct aggcggcagt ttttcgcgaa
    80341 cggtctgttc gataacacgc ggaccggcaa agccgattaa cgctttaggt tcagcgatgt
    80401 tgagatcgcc cagcatggcg aaacttgcag aaacaccgcc catcgtcggg tcggtcagca
    80461 cggagatgta cggcaagccg cgctcctgca tttttgccag tgccgcagag gttttcgcca
    80521 tctgcatcag cgacatcagt gcttcctgca tacgtgcgcc accagaggcg gagaagcaga
    80581 tcagcgggca gttatcttcc tgcgcctgct caacggcacg cacgaaacgt gcacccacaa
    80641 cagaccccat tgaaccgccc ataaaggcga actcgaatgc cgcagcgaca accggcattc
    80701 catacagagt gcctttcatc accaccagcg catctttttc gccggtttct ttctgcgcag
    80761 atgccagacg gtctttatac ttcttggagt cacgaaactt cagctcatct ttcggctcaa
    80821 gctcgctacc cagctccaca aggcttcctt catctaacag gctatgcagg cgattacgcg
    80881 ctgtcatacg catgtgatgg tcacacttcg gacagacctc aagattacgt tccagctcag
    80941 cgcggtataa aacctgaccg cagctatcac acttagtcca caccccttca ggaatgctcg
    81001 ccttgcgggt gggagtaatg ttgcttttaa ttcgttcaat ccagctcatt agggaccttt
    81061 ctgtctgaac ttggttcgat gccagtttta tctttgggga cgcataatgc catttttgcc
    81121 cccaacagac catgaatgtt gcacattaaa acataacagc ccgaaacttt ggataaaaaa
    81181 gtggtcgaac cgcggagtta ctttttattt tgcggcgcgt gccgcagcgc gtttgtgacg
    81241 gattatttcg atgacgcccg gcaaaataga aaccacaata atcccgacga tcagcagctt
    81301 aaggttatcc tgaaccatcg ggattgtacc gaagaaatag cctgcgtagg taaaaagcag
    81361 tacccacaac agtgcgccga tcacgttata agcagcgaaa tgacggtacg acatgtggcc
    81421 cattcccgca acaaacgggg cgaacgttct gacgatcggc acaaaacggg cgagaataat
    81481 cgttttgccg ccatgtttct cataaaactg atgggttttg tcgagataac tgcgacggaa
    81541 aattttcgaa ttgggattac tgaataactt ttcaccgaac aaccgcccga tagtgtaatt
    81601 gaccgcgtca cccacaatcg cggcgatcag catcagcact accatcatat ggacgttgag
    81661 atcgttggtt tccagtgatg ccaacgctcc ggcaacaaac agcaatgaat cacccggtaa
    81721 aaacggcatt accaccagac cggtttcaca gaacaaaatt aagaacaaaa tggcataaac
    81781 ccagacgccg tactccgcga ctagctctgc cagatgcacg tcaatgtgca ggataaaatc
    81841 gatgaggaaa taaatcaggt ccatatttgc ctatgccttg tactcgtcat ctttcaggct
    81901 gtaactacgt tggctacagc ctgaaatgtt ccgagcatta ttctcgttag tccgccagaa
    81961 atagcgggcc cattggcggt tttggaagat cataccggtc agggtaatcc accgcgacca
    82021 gatacagccc ttccgctttt gccgttgctg ccgccagcgt tctgtccttt gccgccagca
    82081 actctgctat ccagctctcc ggctggttgt gggcaccgac ttccatcagg ctgccgacaa
    82141 tattcctgac catatgatgt acaaaggcat tcgctttgat atcaaccacc acataaggac
    82201 cgtgacgcgt gacattaata tgcataacgt tgcgccacgg ggttcgggac tggcactgca
    82261 ccgcacggaa cgaggtgaaa tcattctcgc ccagcaagca ttgcgcagcc cgatgcatcc
    82321 gttcagcgtc cagcggttcg taaaaatggg ttaccccttt actcagtacc gccgggcgca
    82381 gccgatgatt gtagatgatg tagcgataac ggcgagccgt ggcgctaaat cgggcatgaa
    82441 aatcatcagg tacagcttta acccaacgca cggcgatgtc accaggtaaa ttcgcattta
    82501 cgcccaaggt ccacgccgcg tctttgcgct gcgcggttgt ttcgaaatgc accacttgcc
    82561 cggtaccgtg tacccctgcg tcagtacgcc cggcgcagaa gacggtgatg ggttcgttcg
    82621 ccacctggga gagcgccttt tccagcttct cctgcacact gcggacttca ttctgccgtt
    82681 gccagccgta atacttactg ccgtcgtact caatgcccag cgcaatttta taaactggca
    82741 gttgttgctg gtcggacatt agtacagata ctcctgcacc agtttctcgg cgatttttac
    82801 tgccatcagc gcgccgccaa agcgaacgtt atcggccacc gaccagagct ggacttgctc
    82861 cggcataccg tagtcattac gcacgcagcc aacagaaaga tgcggcgtac ccgaagcatc
    82921 ccctacctga gtcgggaatt cgttctcttc agagagcaca atatcttcgc cctgagcaaa
    82981 cgcatcacgc gcttcttctg ccgccagcgg acgcagtgct tcaaagttga ccatctgggc
    83041 atgaccgtag aataccggtg cctggacgac gcttgccgaa atcatcagcc cttcgtcctg
    83101 caggattttg cgtacttcgt caacgatacg acgttcttca cgcacgctac cttcgctatc
    83161 cggcaacaac ggcagcatat tgaacgccag ttgacgaccg aagaaatctt cttcgtcaat
    83221 cggaatgccg ttgagcaatt tcgcactctg ccccgctaac gcatcgaccg cttttttgcc
    83281 ctgggcggag gctgaaatca ggctggtaac actgatacgc gacaaaccgc cctgatcgat
    83341 taacggtttc agtgccgcca gcagctggct ggtcagactg tctggtacgg cgatgacatt
    83401 ccggttgcgg taatcggtca gtacaaacgg gtttacttcc ggcaccacca gcggtacgtc
    83461 gggttcgaga gcaaacagtc cgctgctgtc gatcaccagg caacctgagt tggtcgcttc
    83521 ttcaacccag gcagcggtag cttctttgcc tgcgacaaaa aatgccagct gcgcctgcgt
    83581 ccagtcgaat tcagcggcat cctgcacggt gattgtttta ccaccaaagc gcagttgttc
    83641 gcctgcgctt tcgttacgtg ccagtgcata aatttcccca accgggaact gacgttcagc
    83701 cagcgtttca agcagggctt cgcccatagc gccagttgcg cccaggacgg caatgttcca
    83761 gccttcagac atggtggttt actccagaaa tagcaaagct ccctgccaat gttacagcag
    83821 agagcatgaa gaagagatta acgtgccgga tgatgaacgg cgttaaaacc cagtttacac
    83881 agcaatgatg ccgcactggc gtcatcacaa attacataca gagatgacca ttcacggcgc
    83941 tcaagatagt ttttgcgcag tttatcgaac tcacccggta tcccggcgac tttacgcagc
    84001 ggtgcgtcat cgcggcgcac atcatacacc aaatgcacca gccttttcag cgtcggttga
    84061 tcgagcggac catgcagcgt aatgcggcca aactcaggcg cgggcagcaa cgtatccagc
    84121 gcaacgtgtt gttcatgccc aataaacttg ctataggctt caaacacttg cgtcgtgcca
    84181 cgcgctttac cctccaaggt atagcctgcg atatgcggcg tgccgatatc cacttttgtc
    84241 agcaattcca cattaagttc cggctcgcct tcccagacat ccagcaccac gcttaacttc
    84301 tggccttcat tcaggcaggt cagcagcgcg gtattatcga cgactgcgcc acggcaggcg
    84361 ttaatcagaa tcgctccggg cttcaggcta cggatcagtt tttcatccgc cagatgtagc
    84421 gttttgtacg gaccatcttt aaagagtggc gtatggaaag tcagaatatc cgcgcgctgg
    84481 actaactcat ccagcgagcg gaaatcaccc tcatccccac ggtcggcgcg aggcggatcg
    84541 caaagtaagg ttttaatccc taacgcttcc agtcgcgcct gcaatcgacg tccaacgtta
    84601 ccaacgccca cgatccccac cgtacggtcg tgcagtgaaa atccatcgcg ttcggcaagc
    84661 atcagcaggg aggagaaaac atattccacc accgcaatcg cattacagcc aggtgcagcg
    84721 gaaaaaccaa ttcccgcctg cttcaaccat gcttcatcga catggtcggt ccccgcagtg
    84781 gcagttccaa caaatttaat gggttttcct gccagcaaag attcattcac tttcgtgacc
    84841 gaacgcacca tcagcgcatc cgcgtctgcc agttgagcga cggggattgg acgcccggga
    84901 accgcgatca cctcacccaa acggctaaat aagtcgcggg cataaggcat attttcatca
    84961 acaaggattt tcacgtttgt gttacctgta tgagacgaga gttaaccgga caagtgtgcc
    85021 ataatctcgc ggccaggcat acttgcgaag atttcaggta taaggatacg taatgataca
    85081 acctatttcc ggccctcctc ttgggcagcc accaggtcag ggagataacc taccgtctgg
    85141 cgcgggcaat cagcctttat ccagtcagca acgtacttcg ctggaaagct taatgacgaa
    85201 agtgacctca ctgacgcaac aacaaagagc agaactgtgg gcgggtatca ggcacgatat
    85261 tggtctgtcg ggagattcac cgctgctttc gcgtcacttc cctgccgctg agcataatct
    85321 ggcgcaacgt ctgctggccg cgcaaaaaag ccattctgcc cgccagcttt tagcgcaatt
    85381 aggggagtat ttacgtctgg ggaataatcg tcaggcggtc acggattata tccgtcataa
    85441 ctttggtcag acgccgctga atcagctctc accggagcaa ttaaaaacca ttctcaccct
    85501 gttgcaggaa gggaagatgg ttattccgca accacagcag cgcgaggcga ccgaccgtcc
    85561 tttattaccg gcggagcaca atgcgctaaa acagctggtg accaaacttg cggcggcaac
    85621 gggggaaccc agcaaacaga tctggcaatc gatgctggaa ctttccgggg tgaaagatgg
    85681 cgagttaatt ccagcgaaac tgtttaacca tctggtgacc tggctacagg cgcgtcagac
    85741 gctaagccag caaaatacgc cgacgctgga atcactacag atggcgctaa aacaaccttt
    85801 agatgccagt gaactggcgg cgttatcggc atatatccag caaaaatatg gcctttctgc
    85861 gcaatcatcg ctttcttctg cccaggccga ggatattctt aatcagcttt atcaacggcg
    85921 ggttaaaggg attgatccgc gtgatatgca accgctgctt aatccttttc caccgatgat
    85981 ggacacgttg caaaatatgg caacgcgtcc cgcgctgtgg atactgttag tcgcgattat
    86041 cctgatgctg gtctggctgg ttcgttaacc ccgacgaaat gacagtatcg tgacaataat
    86101 acccagcacc gcagagatcg ccccggcaag aaataccgaa gagtaaccaa acgtggtcgc
    86161 cagcattccc gccagcggcc cggagacgcc gagggcgata tcctgaaacg cggcgtaacc
    86221 gcccagtgcg gtgccgcgaa cttgtgaggg gacgcgttta accacctcca cgcccagcgc
    86281 aggaaagata agcgaacatc cggctccggt taacgccgcg cccgctaatg cgacccatgc
    86341 acctggggct tgccagagca gcaacaagcc caccgtttct acaagcagag agacaatcgc
    86401 cactttcacg ccgccaaaac ggtccggcat ccagccaaac atgacgcgca tcacgacaaa
    86461 tgcgccgcca aacgcggtaa gagtaaagcc cgccatcgcc catcctttgc tggcaaagca
    86521 gagcgaaacg aaagtcccga taaccgcaaa accaacgcct tgtagtgcca gccctaaccc
    86581 tggtttccag ataagcccga caacgctcca cagcgatgga cgttctcccg ccagggccgg
    86641 tactttgcgc actgtgccgt tacaggccca cgccagtaag ggtaatacca ttgtggtgat
    86701 cgccagtgcg gcaaaaccgt aatggctatg aatcaacagg ccaagcggcg caccaacagc
    86761 gagggcaccg taaatcgcca ttccgttcca tgacatcact ttgccagagt gttttggccc
    86821 tacgatgcct aacccccagg tcagagcgcc tgtcagtaac tggctttcac caaacccaag
    86881 aatcaaacgc ccgatgacca acagggcaaa tttgaacggt gcggagacag gcaaaatcgc
    86941 cgccagcagc aacgcgccgc cagccagacc acaagctaac atcccctgaa gcgccgaacg
    87001 ttttgcgcca tattgatcgg ccagtcgccc ggcgtaacca cgcgtcagca ccgtagccag
    87061 aaactgaatc ccgacggcaa tgccgaccat ggtattgcca tagcccagat catgatgaac
    87121 aaacagcggg ataaccggca acggcaaccc tacggtcatg taggtgagaa aaaccgcaaa
    87181 agcgatgcgg aagagcgaaa aactggcaga agatcgtgtt tcggtttggc ttacagcagt
    87241 catgcattac tccagaatgc agcgcaaggc gaggagtatc cccgtctcat ctctctggtt
    87301 tcagggttac ggtgcgttgg caggatttaa cgcgtacgtc ttttcagaag gaaatcgaca
    87361 aagcgggaag tttgcctaga actggtggcg attgtcaatg atgtgaaaaa gggaaccatc
    87421 aggttccctt ttgcgttagt gccggaggcc gcattggcgc gtaacgtcgg atgcgacgct
    87481 ggcgcgtctt atccgaccta ctccggccta ctgcgaatta atctttcagc ttgcgcatta
    87541 ccagcgtggc gttggtgccg ccgaagccga agctgttaga cataacagtg gtcagttcgc
    87601 gatcggtcgt ttcggtcacg atgttcagac ccgcagcctg ctcgtccagc tcttcaatgt
    87661 tgatgctcgg ggcgataaag ccgtgttcca gcatcagcag agagtagata gcttcctgca
    87721 cacccgcagc gcccagtgag tgaccggtca tggctttggt tgcagaaatc gccgggctct
    87781 tatcgccgaa cacttcacgg atagctgcca gctctttcac gtcgccaacc ggagtcgaag
    87841 taccgtgaga gttcaggtaa tcgattggag tatcaacgcc atgcatcgcc atctgcatac
    87901 aacgtactgc gccttcgcca gacggagcaa ccatgtctgc accatcagag gttgcgccgt
    87961 agccaacgat ttcagcatag atgtaagcac cacgcgccag cgcgtgttcc agctcttcaa
    88021 ccactaccat accgccgccg ccagcgataa cgaaaccgtc acggtgagcg tcgtaagtac
    88081 gggaggcttt ttccggggtg tcgttgtatt tagtagacag cgcacccatc gcgtcgaatt
    88141 cgcaggccat ttcccagcac agctcttcgc cgccgccagc aaacacgatg tcctgtttgc
    88201 ccagttggat ctgctctact gcgttaccga tacagtgtgc ggaagtcgca cacgcggagc
    88261 tgatggagta gttaacgcca tgaattttaa acggcgtggc gagacaggca gaaacgccgg
    88321 atgccatcgc tttagtgacc acatacgggc caaccgcttt caggccgcgc gggccgcgca
    88381 ttgcgtcagc gccgaacacc tggaaacgcg gggaaccgcc gccggaacct gcaatcaggc
    88441 caacgcgcgg gttattctgg taagcttccg gagagaggcc cgcatcagcg attgcctgct
    88501 ccatagaaag gaatgcataa atggatgcgt cgctcataaa gcgcacaact ttgcggtcaa
    88561 tgaggccagt ggtatccagt tttatgttgc cccagacgtg gctacgcatg ccggaatcct
    88621 tcagctcctg agagaaagtg atccctgaac gtccttcacg cagagatgcc aggacttcct
    88681 gctggttatt accgatgctg gaaacaatgc ccaggccagt aatcactgca cgtttcattc
    88741 aatacctctg taagtcgcac atagagtaag tttcgaatgc acaatagcgt acacttgtac
    88801 gccgaacaag tccgatcagc catttaatag agaaatttgc gcagccttac acacatcgct
    88861 aagatcgagc caccgcctgt aagacgagta acttacgtga aacactactc catacaacct
    88921 gccaacctcg aatttaatgc tgagggtaca cctgtttccc gagattttga cgatgtctat
    88981 ttttccaacg ataacgggct ggaagagacg cgttatgttt ttctgggagg taaccaatta
    89041 gaggtacgct ttcctgagca tccacatcct ctgtttgtgg tagcagagag cggcttcggc
    89101 accggattaa acttcctgac gctatggcag gcatttgatc agtttcgcga agcgcatccg
    89161 caagcgcaat tacaacgctt acatttcatt agttttgaga aatttcccct cacccgtgcg
    89221 gatttagcct tagcgcatca acactggccg gaactggctc cgtgggcaga acaacttcag
    89281 gcgcagtggc caatgccctt gcccggttgc catcgtttat tgctcgatga aggccgcgtg
    89341 acgctggatt tatggtttgg cgatattaac gaactgacca gccaactgga cgattcgcta
    89401 aatcaaaaag tagatgcctg gtttctggac ggctttgcgc cagctaaaaa cccggatatg
    89461 tggacgcaaa atctgtttaa cgccatggca aggttggcgc gtccaggcgg cacgctggcg
    89521 acatttacgt ctgccggttt tgtccgccgc ggtttgcagg aagccggatt cacgatgcaa
    89581 aaacgtaagg gctttgggcg caaacgggaa atgctttgtg gggtgatgga acagacatta
    89641 ccactcccct gctccacgcc gtggtttaat cgtacgggca gcagcaaacg ggaagtagcg
    89701 attatcggcg gtggtattgc cagcgcgttg ttgtcgctgg cgttattacg gcgcggctgg
    89761 caggtagcgc tttattgcgc ggatgaagcc cccgcgctgg gtgcttccgg caatcgccag
    89821 ggggcgctgt atccgttatt aagcaaacac gatgaggcgc taaaccgctt tttctctaat
    89881 gcgtttactt ttgctcgtcg gttttacgac caattacccg ttaaatttga tcatgactgg
    89941 tgcggcgtca cgcagttagg ctgggatgag aaaagccagc ataaaatcgc acagatgttg
    90001 tcaatggatt tacccgcaga actggctgta gccgttgagg caaatgcggt tgaacaaatt
    90061 acgggcgttg cgacaaattg cagcggcatt acttatccgc aaggtggttg gctgtgccca
    90121 gcagaactga cccgtaatgt gctggaactg gcgcaacagc agggtttgca gatttattat
    90181 caatatcagt tacagaattt atcccgtaag gatgactgtt ggttgttgaa ttttgcagga
    90241 gatcagcaag caacacacag cgtagtggta ctggcgaacg ggcatcaaat cagccgtttc
    90301 agccaaacgt cgactctccc ggtgtattcg gttgccgggc aggtcagcca tattccgaca
    90361 acgccggaac tggcagagct gaagcaggtg ctgtgctatg acggttatct cacgccacaa
    90421 aatccggcga atcaacatca ttgtattggt gccagttatc atcgcggcag cgaagatacg
    90481 gcgtacagtg aggacgatca gcagcagaat cgccagcggt tgattgattg tttcccgcag
    90541 gcacagtggg caaaagaggt tgatgtcagt gataaagagg cgcgctgcgg tgtgcgttgt
    90601 gccacccgcg atcatctgcc aatggtaggc aatgttcccg attatgaggc aacactcgtg
    90661 gaatatgcgt cgttggcgga gcaaaaagat gaggcggtaa gcgcgccggt ttttgacgat
    90721 ctctttatgt ttgcggcttt aggttctcgc ggtttgtgtt ctgccccgct gtgtgccgag
    90781 attctggcgg cgcagatgag cgacgaaccg attccgatgg atgccagtac gctggcggcg
    90841 ttaaacccga atcggttatg ggtgcggaaa ttgttgaagg gtaaagcggt taaggcgcgg
    90901 taatctgctc tcgcagtgtt tgccggatgc ggcgtgaacg ccttatccaa cctacgtgtg
    90961 acgtgtaggc ctgataagac gcgataagcg tcgcatcagg catggtgctc cagacgccgc
    91021 aacttacagc tgtgacgcct gctgaaacag gttttcccac atatcggtca ccagcgcctg
    91081 gtcacgcggc gacaattcac cggcaccaat ggctttttcc agactctggc taacggtcgt
    91141 atgcaccgcc tgagcggagt ggtcatcacc actttccagt tctgcgatgg ctaatgtcag
    91201 gtggccacgc aaatacccac tggcaaacaa ctcatcatca ctggcatggt caaccatgcc
    91261 gtcgattaat gccagaatgc gtgattcaaa ctccgcgatc atcttctttc ctcattgtaa
    91321 aacgtggcgc tggcttcagt tgagcgcttc cggccacggg aactgttccg ccgtaagttc
    91381 cggcgtgtga taataatttt gtagtgcttt aatgaagcgt gccgggcgtt ccggaatgcc
    91441 atttgtcaga taatccatca cctgcgcatg tacccgccgt tgaaacacca cacgatccgg
    91501 ttcgaaatcc ccttccagat tgtcgcaact gacgttaaac ggatatccag ccgccacgca
    91561 gaataaccag tcgaacgcct gcggcttcac ttcaacatct tcaaactggc tttgcgtctg
    91621 ggcatcgcgt ccgtccgggc aataccagta gccgaaatca accagctcac ggcgcgcttt
    91681 tccggcaata caccagtgcg aaatctcatg aattgcgctg gcataaaagc catgagcaaa
    91741 gacgattcgg ttatacggta cttccgcatc agcaggaaga tagatcggtt cgtcgtcgcc
    91801 tttaatcaga cgggtattaa attcatcggc aaagcagcta ttaaaaattt caatcaactg
    91861 ctcgtagtgg tgtgtactgt tcattagttc atccccaacc agtggaggat ctcctgtccg
    91921 tggctatcgt aaagtaattt ggcactcatc accgccgaga cgataacaat catcgggcgg
    91981 atcagctttt gtcctttgct caacaccaac cgtgaaccca tgcgcgcgcc gaggaactgc
    92041 cccaccagca tgacaaagcc cgtcgcccaa atcactttgc cgccgagaat aaacagcagc
    92101 agaccgccaa tgtttgacgt tgcgttgagt aatttggcgt gagccgtggc tttggcgagg
    92161 ttgaatccgc acagcataac gaaggccagc gcgtaaaacg atccggcagc cgggccaaag
    92221 aatccgtcat aaaaaccgac gcagccaccg gcaattaacg caaagggtaa accgtacatc
    92281 cggcgctggc gatcttcttc acccagcttt ggcatcagca agaaatagag gccgatacaa
    92341 atcaccagaa tgggcaaaat ctgccgtaag acatcagcct gaacgtactg taccaacagt
    92401 gcgccgctca ttgagccgac aaaggtcatg gcaatattga gtttctgatc gcttaaacta
    92461 accactttgc ggcgaataaa gtagatagtg gcggaaatag agccgccgca ggcttgcagt
    92521 ttattggttg ccagcgcgtt agcgggagac attcccgcag ccatcaatgc cggaatggtg
    92581 agtaacccac cgccaccggc aatcgagtcg ataaatcccg ccagcatggc gacaaaaaag
    92641 agaactccca gcaacagcgg agaaaccata aacaggctat taaacgtttc cattagatca
    92701 cgtgctcatc cagtagcgcc tggcaggaag gcggcaacgg aggcggtgtc ttcttctcag
    92761 gctttgttgt tcccggtttt ggaggttcaa accagctttg cagttctgcc ccgcaaccat
    92821 cgcctgatgg cggtaaaggt tgatcttcac actccagact atcggcaggg caacgtaatc
    92881 gtacatgcat atgcgcgcga tgctggaacc agggtcgcac tttgcgcaac cagtcgcgat
    92941 cggtgcccgc atcaaggcaa agttgttgtt taatcgccgg attaacaaaa atgcgcgtga
    93001 cgtctttgtc ctgggcggcg agtttgatca agctgaaaat ttctggcttc cacagcgtgg
    93061 agacaacgtg tttaccgtcg cgggaaacta agtccagtgc ttgcgggcgc aagagctgcg
    93121 cggaggtcca gcgagttttc ggcagttgca gaaagatatc gacatccagt ccggtctggt
    93181 ggctggcatg accgccgttg aaacgcccac cagcgggcat ccccatatcg ccaatcagca
    93241 ccgtacccat gcccagattg ctcacctggc tactcagacg ctggataaac atcaccagat
    93301 ccgggtgacc gaaatagcga cgctgatcgg tacgcatgac ctgataatgt tcggactgta
    93361 tcggcaacgt gtcagcgccg acaatacagc cattagaaaa actgcctatc gattgtgcgc
    93421 tacccggcac aggttgggtt attttttgcc acggcgtcgc tgccaggcta gcgctactgg
    93481 caagcagagc cagcagcgca atcgcggttt tgttcatttt ttaccagcgt ggaatatcag
    93541 tcttcacatc ggcattttgc gcccgctgcc gtaacaggtg atccattaaa acgatcgcca
    93601 gcatcgcttc agcgatcggc actgcacgga tcccgacaca gggatcgtga cggcctttgg
    93661 tgatcatctc aacttcttcg ccaaagcggt taatagtgcg ccccggcacg gtaatgctgg
    93721 aggtcggttt cagcgccata tgagcaatga tttgctgccc gctgctgata ccgccgaaaa
    93781 tgccgcccgc atggttgctc tggaaaccct ctttggtgat ttcgtcgcgg ttctggctgc
    93841 cgcgcagcgc cactacgtca aaaccgtcgc caatttccac gcctttcacc gcgttgatgc
    93901 tcatcagcgc atgggcgatg tcggcatcca ggcggtcaaa gaccggctcg ccaagtccgg
    93961 cggggacacc actggcaaca acggtgactt tcgcgccgat ggagtcgccc tcttttttca
    94021 gcgcgcgcat cagttcatct aacgcgtcga ttttgtcggg gtccgggcag aagaacggat
    94081 tttgttcaac cagcgaccag tctttgattt ccagcggaat gtcgcccatc tgggtcaggc
    94141 agccgcggat ttcaatacca aatttctcgg cgagatattt tttggcaatc gcccctgccg
    94201 ccacgcgcat ggcggtttcg cgggcggaag aacgtccacc gccgcgataa tcgcgcagac
    94261 cgtatttttg ttcgtaggtg taatcagcat ggcctggacg gaagacgtct ttaattgcgc
    94321 tgtaatcctg agaacgctgg tcggtatttt cgatcaacaa gccaatgctg gtgccagtag
    94381 taacgccttc aaaaacaccg gagagtattt tgacctgatc cggctcgcgg cgctgggtgg
    94441 tatagcgcga tgtcccaggg cgacgacgat caaggtcatg ttgcaggtcc gcttccgtca
    94501 gcggaatgcc tggcggaaca ccatcgacga tgcagccgag cgccagcccg tgcgattcgc
    94561 cgaaggtggt tacgcgaaag agttgtccaa ttgtgtttcc agccatcacg gctccgttat
    94621 tgttgtgttt gcgtgtttac ttaatcttta taaatcgcga aatgttctcg tgcggcaaga
    94681 agctgctctt tagtgagcat aaacacacca tcgccgccgt tatcaaactc cagccaggtg
    94741 aacggaacat cgggatattg ttccataaga tgtaccatgc tgttgcccac ttcacaaatc
    94801 aacacgccat catcagcaag gtaatctgcc gcgttaccga gaatgcgacg cgtcagtttc
    94861 aggccgtcgg tgccagatgc caggcccagt tccggctcgt gacggtattc gtttggcagg
    94921 tcggacatat cttccgcgtc gacatacggc gggttagtga caatcagatc gtattgcact
    94981 ttcggcaaat cacggaacag atcggaacga atcggaatga cgttgtggat cagaccgtgt
    95041 tcttcgatgt tctgttcggc aaccgccagc gcgtcgggag agatatctac cgcgtcgact
    95101 tccgcttccg ggaaggcata agcacaggca atggcgatgc agccgctacc ggtacacata
    95161 tctaaaatat gctgcggttg cttgctgatg agtccggcaa atttattgtt gatcagttca
    95221 ccaatcggcg agcgcggcac cagcacgcgt tcatcgacgt aaaattcatg accgcagaac
    95281 caggctttgt tggtcaggta agccaccgga atgcgctcgt tgacgcggcg gatcacgcgt
    95341 tcaacaatac ggtgtttttc gctggaggtc agacgcgcgg tgcgcatatc ttccgggata
    95401 tccagcggca ggtagagcga tggtaacacc agctgtacgg cttcatccca cgggttatcg
    95461 gtgccgtgac cgtaccagat atttgccgcg ctgaagcggc ttaccgacca gcgcaacatg
    95521 tcctgaatgg tttgcagctc atttactgct tcatcaacga aaattttatc cacgtattcc
    95581 tccagggcat gatcgcaata atttcggcgg ctagtttgcc atgaagatga cgataaatca
    95641 gcattcacgc gcggtgagtg aggaaaaata cgtttaaaac gatcgattgt gctacgagtc
    95701 gggtaaactg taggaaaatt agaaatagag acagataaat gaaaaagaaa acaacactta
    95761 gcgaggagga ccaggctctg tttcgccagt tgatggcggg gactcgcaag attaagcagg
    95821 acacgattgt ccaccgaccg cagcgtaaaa aaatcagcga agtgccggtg aaacgcttga
    95881 tccaggagca ggctgatgcc agccattatt tctccgatga gtttcagccg ttattaaata
    95941 ccgaaggtcc ggtgaaatat gttcgcccgg atgtcagcca ttttgaggcg aagaaactgc
    96001 gccgcggcga ttattcaccg gaattgtttt tggatttaca cggtctgacg cagctgcagg
    96061 ccaagcagga actgggcgcg ttgattgccg cctgtcgccg cgaacatgtg ttttgtgcct
    96121 gcgtgatgca tgggcacggg aagcatattt tgaagcaaca aacaccgctg tggctggcac
    96181 aacatccgca tgtgatggca tttcaccagg caccgaaaga gtacggcggt gatgcggctt
    96241 tgttggtgtt gattgaagtg gaagagtggt tgccgccgga gttgccctga atgctggcag
    96301 caaatgccgg agcagcgtaa acgccttatc cggcctacaa atatgtgata attcaataaa
    96361 ttgcaattca cttcgtaggc ctgataagac gcatcagagc aatactacga acttcagttc
    96421 attcaataca tactgaaaac gacacgtcga atcaatcggt tgcccactac tggtattcac
    96481 attcaccaca atatcgtgta gtactttatc cgttaccgga tgacgagttg gcgtaaccgt
    96541 taccgttgat ttatcacaac cgtaagaggt aaaggttaac aattctgata ccgcgcggca
    96601 gtatgctcct ctgccatcat tatcagaaaa attactgcca tcacagagtc tgccgccttc
    96661 catcaggtag gtcgaaagcg tggtggtcac tgttgacccc gtggactgaa ttcgtaacgc
    96721 cttattatgg ctgacataat tttcactcca ggagatatcg tatgaagcga ccggtatcgt
    96781 tgggcatatt gtgttatgca ctgtaggggc cgtatagctt ccaaatccgg gtaaatctgt
    96841 ataggaataa aaggatgcga tccaagggca accataatca tcagatgcaa cggtaatttt
    96901 aaacgtcata tatgcacaac gaaaaatttt cgtatctatg ccagggcaat ttgagttatt
    96961 aattgtatca aagtcagtca gtaccaaagt tggagctgaa cccgacccag accaggaaat
    97021 aacccgcaac gtatcgcctt tatacgcaaa agaatcatat ttcccatatg caacctcctt
    97081 tggcgttatc accgtaacat tgaaaacact gctcgctaca gcaccggtta ccttgtccat
    97141 ggcataataa actctcacac tgtcaaaact cgcactcata agtttatctc cagcataact
    97201 cttagatgcg ccgacaacac tcagaacgag cagcattagg ctcgcgaaaa ttatggtcca
    97261 ttttttcatt tcagaaatac tcaaaggttg ccgtcactgt ggcactaaag tctccggacg
    97321 ttacatctcg cccgctcttt gcctgtaact aggtattaaa atttaggctg ttatttccct
    97381 gattcagcgg gagcgtgacg cctgttgtat tgttgattgg caccgatgtg cctgccgcag
    97441 tttcaatacc aatcccagcg ccggatgctg atgacgagga gtcaaaggcc agaaagccgg
    97501 gtagagcact atcttctgtt cctgtcaacg ttactttgac attaaacaat gtagaactat
    97561 gacaatcttt caattgaaac accaatggca ctcgctctga ctgcccaagg ttcattaaat
    97621 cgctggcggc aatggcagga aaatgtactt ctgccaactg gctaccatta atcactggcg
    97681 tacacgattt actgattaaa ttgccggtaa agtgcaaatt atctcctgct attgtggttc
    97741 cactgcccag caacatcagg ctactaataa aaaacaacgt acgctttttc ttcattggta
    97801 atctacctgt aacgtggccc aggcctcaaa gtccgcctct ggcagaatga caccactctt
    97861 ttttactggc acagccgtta acaccggtaa atcggtttca ttcaccacaa gtggcgtatt
    97921 gatcgtaaaa ctctgccctg cctgtttgag ctgaatgcct aaaccagtga tattgctgct
    97981 tactgcagca ttatcaaaat cgcttggtgt gccactccag ctcaaggtca tttccattaa
    98041 tgggtcgcga accgccgtat cgcaggtaat ggaatacggc acggtttgct cataattgct
    98101 gccattaatg tcatcaataa gcacatcctg aaaagttatt tcaatggttt gagcgttgct
    98161 aatcgtgcag ttaggcggct gcactaaata accgtgaaaa gagacgtttg agagtgccat
    98221 cgagcaaaag ccccacagca aaataagcga gatccgtttc attggtaatc caccacaatt
    98281 gtggcactgg cgttaaattc agctgccgct aacgttgtac cactctgttt caccggcaca
    98341 gcttccagaa cgggaactcc cgaggaaaaa ttaaatggca accatgagcc agaggttaag
    98401 tcgagtattg tgtgatcact acttttctga acccgaatac caaacccagg aatacccgtg
    98461 ctgataactg tttcaccgtt aatcacgacc gttgtcgcct gcatctgtag acgcaggtca
    98521 tccattgcca gattgttaca aacaagcgag tagtcaatgg gacgtttata atcaacccta
    98581 ttgatcttat taatgaatac attaccaaac tcaactgccg caccatttac cgtacaagga
    98641 ggaggggcat cgacaagcac ccgtaaattc atacttactg acttactcgc tgcaactgca
    98701 aagccgctac caacacaaca tatcagtaac ccagttaccg cccagacaga acaagatcct
    98761 ttcatactgg ctctcccggc ctcagccttt gcggctctgc tcttcatcga cctagcaact
    98821 gttaccgttg caagtgaaaa agagcggcat ccgagcgcca tagtcgttga cataggtcag
    98881 taccggcacg ctacccatct tcacattcag cggtgcagag gttttcggcg acatcactat
    98941 tggcgagaaa ccggctgccg gattgccatt tttctgtgtg ctggcattac tgaaaatgac
    99001 gtaatacgac gtcgggttat tgacggtaaa ttcctgaccg ttgcgggtta aagtaacctt
    99061 atattgatag gggtttttca tgctgacgtt ctccagcgcc ttcggtcgcc agaacaactt
    99121 aatacgcgtt tgtagtgcta tttgcagcgt gttggctttg tttgattttg gcgggatctc
    99181 acgcacgttg aagtagaaca ggctttcacg atcggcaggc aatttgttga tatccggcat
    99241 tccctgcact tttacctgcc cgttcatcat tgaatcgatg cgttgcaccg gtggcagaac
    99301 agttaacggt gaactgatct tgttaccctt ttcatcttcg atccagcttt gggccagata
    99361 aggcagtttc ggatcgttat tgcgcaacgt cacgctgatt gatttgtcgc tttcattaaa
    99421 tatcaaacgg gtacgatctg gcgttacaga agcctgggca gacaaactgg cagcgctaag
    99481 cagtaacgct agcgtcgtag cacccaattt ggcagagcaa agtaaatcag acatggatca
    99541 ttccttaatc acgttattgg ttagctgaaa cagaaacagg gggattggtg ggtgtcacct
    99601 gttgcgtctg ctcctgaatg acaggcttta tgtcatcagg aacaaccggc gcgacgttgc
    99661 ctttatgctg gcatggcagt aacaagccgt taaacagatc ggcaggcagc gggtccggca
    99721 ggttgatatc gcaatgcgca acaccactcc agaacacact catgtgttcg ccaggtttca
    99781 ccccagccag ataaacattg ccgtcatcat cgacaaggcc cactgtctgc tcgttatcat
    99841 tttttacttc tgcgccaaac ggaggatggc tgccgtcttg taaacgcagc accgccatcg
    99901 ctttttgacc actgatgacg gcaaatttgc ggtagccaat cgccccttca gttagcgtgg
    99961 cttgtaccac cgactgggtt gcttcggcgt tttccggcag tttgttgagg tcgatatacg
   100021 cctgattgcg gtaatagtta ttgacgtcag aaacgacggc tttaccaaac atattggtat
   100081 aaacagcagc accgttacct tctaccggaa catcggcaac gccatcggca tcaatcaaca
   100141 agcgtgtccc gcccatattc tgggtacggt gaagtgcgcc accgtgggca gtaaacgttg
   100201 cgccgccctg taacgacaag cccgcagagg tgtattgccc ttcatggtag ttcgcactga
   100261 ggtcaacctg cgccagcgaa ccatcatggc tgtaatagcc gtcaacgctg gtgtgtttgt
   100321 cactggtgcc gacgttcaac tgatagtgag tcgcgtcatc gacacggctg aaataaccga
   100381 cctgactgct gtccgtccca ctgccatagt accgttatag ctaacggtac tgttgtcgcc
   100441 ccacggcata ctgagcgaaa tgtacatgcc tttgtcggcc tggttgtcat actcgtaacg
   100501 gtagccagtc atcgagatgc tgacattacg aatactgccc atattgaaat agtgcgagag
   100561 catgatgttg tagtttgtct gctcctcgcg atcccagtag gtatggcggg tatagttgag
   100621 ataaaccgaa acacccgcat cgcggaagtt ctggttataa gtggcggtgt acatctcttt
   100681 gtcgttgccc gtgcgtacca ttccgctgtc actggcatcc agatactcgc tcatggtcat
   100741 aaagttctct tccgagaagc gatatccagc aaaagtaacg cggctgttga gctggtcaaa
   100801 gtctttggaa taactcacac ggaaggagtt accgtccagc gaacctttgc cataagcggt
   100861 gtctttatcc agtttggtgt gcgagtgagt aacatcaaac gcgaccgcgc cgaatgtaga
   100921 caaatcgcga ccgacaccaa gcgccgcaga ctgatagttt tcatctccca gtgcgccgcc
   100981 atataacgac cagccgttag cgatccccca ggaagcttca gcaccagaaa aaaattcacc
   101041 ctcgacatgg tgtccccact cttgcggacg gcccatcatg atcttatagc gaacctgacc
   101101 tggacgagtg aggtatggca tcgaggcggt gctgatgtca tattcctgca cctggccgtt
   101161 ctgttcttca atgcgaatat gcaacgtacc ggagacagaa tcaccaagat cctgaatacg
   101221 aaacggtcca gccggaacct gcgtttcgta aatcacacgc cccatctggc tgacggtcac
   101281 ttttgctgtg gtgtgtgcca cgccggaaat gtctggcgcg tagccgcgca gattgggagg
   101341 caacatttga tcgtcagtac tgacactgcc accaacatag ttaaaaccat caaaaatatc
   101401 ggatctgagg taatcctcgc ccagcgccag tttggctttt aatgatggta acgcccgcct
   101461 ggcatagtag cgactccact cccatttttt ttgagtttca tcgccgctga attcatcgtc
   101521 atcattactg cgagtatgtt gatagttagt ctgccagtca gcacgcatac gccacggccc
   101581 taggttaacc ccaaccgtcc cgttgccgct gatctcgtta ctatcatcac cgccattttc
   101641 ttcgtgccgg gtttgtgcgt tgatgctgta gtccgcgacg atcccggaga tgccgtcatc
   101701 ccaacgtgaa ggcggatccc agtcgggata agtatattcg aggtaagcct gtggcagtga
   101761 aatgactaat gcggactggc ttaaatcagc cttaatttcc atgccttcca gttgaccgga
   101821 tttcaggcat ttagcatcgt ggctccattg cagatttttc gccacgtctt cttttaaacc
   101881 aaactgcgct accagttccg gtgtcagaca agcatagctt ttgctcgcgt catcttcacc
   101941 agcataccag taaatatcgt actcttccgc caatggctgt ttatttagtt gaacctgtaa
   102001 attgtatttt ccgggctcaa catatccctg gctggaaaaa cgcttcagat caatttttgt
   102061 gtcgcctttt aattccagaa aacgggaatc aaactgaatg tcgtcttcag cccagacact
   102121 gctataactc cctgacattg ccagcgcaat gcaccacgga agaatccgta atcgaaaaag
   102181 agaatggtca ggcatacacg tttctatccg tttttattct tgttcggtca aaatccattt
   102241 aaatccccca tagcagtgtc gctatggggg tatgccttac aggtaagtaa tctggaaggt
   102301 ggtatttgct tcaaaattac ccagatctgg cgcatcagct gcgccgacca gccaggcttt
   102361 aaagttcagt gtctgcttcg ctttgccttt gttggtagaa gtgtctttca ctattttctg
   102421 gtcaataccc atgccgcttt tgtaagaggt acccagagag tcaccgatcg ccaggctaac
   102481 attgttaaat gccgcaccgg tatcggtatt gaaaatggtg taataattgc cgctatttgc
   102541 agaagaaacg gtaccagtaa aggtagtggt cattgtttcc tgagtatcaa atacgcaatc
   102601 ttgcagacga atctggaaac ttttcggcgt ggtggtgccg ttatttttca gtttttcggc
   102661 accgatatca cccatatcaa cttccagttt gtgatcgtcc ggaacgatag aacacactga
   102721 gtcttcaatg gtgccgtaaa aacgtgcagt accgttgtta cccgcagcga ttgttgccgt
   102781 agaggtgaac acgcccatca ccattgccgc agcaatagct gttttaacaa acttactcat
   102841 tatttgtcct taaactataa ataattaaaa atataaacat tgcaatacat tgattcagtc
   102901 aatagccaat gtttataccc agccattgaa tacggcatgc gacccaaaaa gaacaggtcg
   102961 tagaaaattg tattcattgg tgtggggccg catagtgatg tttccacacc cctgatccaa
   103021 tttaatttta ctaaagagta aatgtagatt taattaatat attgacagaa gaaaggttat
   103081 cttaaggaca tccttaaaaa atacggaata agtatttaga aaaaatagaa agtatatcaa
   103141 taatgaatat caacagctta aaaaacaaac aatgaacata taatgcgatt ggcatttatc
   103201 cacttttgac aatataatat ataaacacta tagccagaat ttaatcaagt ttcaattaca
   103261 tcctgcctga ttattcaact ctattttctc tattatctgt attaataaat gacactgagt
   103321 gtcaaacaga cattattcat ttcaacatgt aactttgggc aaaatcaaga tacgcgcaga
   103381 agaataattt ccgaatgaat gtagaaagtg ttttatttat agataaggag agtcttcgat
   103441 agtagtgagg ttgacagata tccgctgcct aatgagcaat tatgcccaat gaatctacct
   103501 cattgggcat aatttggaac acatcagata gcttttgcca tcttcagatt acacgggctc
   103561 atctgccagt tgaatgtacc gtttccgctt tcatcaagcg ttacgctggc aatggccgag
   103621 gtggtaaaca taggcggcgt ttcgcccgga cataactcgg caaccagata cccgactaac
   103681 ggtaagtgag agatcaccag taccgaagca acaccttcat tggtcaacgc ctgcaaatag
   103741 gcactgacca gaccaacatc gccgcagggc gttaactccg gcagaacttc tgcactggaa
   103801 ggcaggttca gacaatcccc tacttcttcc agtgtttgct cggctcgcag gaacgggctc
   103861 accagaacgc gttcgatttc cactttttga cctttcagcc agttcgccat caggcgagat
   103921 tcgtcacaac cattagtggt cagaggacga accgaatcac tggcggcatc gagggctgcg
   103981 tcgccgtgac gcatgataaa aacttgcata ttgcaccgct tttgttaacc agattcgccc
   104041 gttttctttc atctgaaacc agagaaagaa aacggtggcc ggcattgtgc cttatccatt
   104101 caccgaatga aacgctgttt tttacctcaa tggcgtaagt atagtcaata tgcgtttaca
   104161 ttttgaccaa cactccgcca ttcagcgcgg attcatatag ctttgacctt cttattgcag
   104221 gtcagttgca gttgttttcc aaaaactttc cccacgcgcg cccatctcga ccaaacgctc
   104281 gcaaggggta aaacgggaac catactgcgt ggccagtcgt tgcattattg caaccacttc
   104341 gcccgcgccg agagaatcga tatagcggaa cggtccaccg agaaatggcg gaaaaccaat
   104401 gccaaatacc gcaccaatat ccccgtcacg cacgctacgg ataacctgct catcaacaca
   104461 acgtactgct tcattcagca tcaacatcac acaccgttca gcaacctgcg gtgcggagag
   104521 tcgcccctgc ccttgtgcgc caatgagcgg gtaaatggca ggatcgacct gttttttgct
   104581 tttacgccct ttctgaccat aaagatagaa accccgacca ttttttctgc ctttgcgatc
   104641 gtcgttcaaa attgaagaaa caacatttgc aggcgcgcta aaacgttctc cataagcggc
   104701 ttccagtaca ggaataattt tagtcccggt gtcgattcct acctcatcca aaagttggat
   104761 tgggcctacc ggaaaaccaa atttcactag cgcggcatca atgtgctcta cccgttcacc
   104821 ttcggtcagc atgcggatag cttcattaat gtaaggcgct aagatgcgat tgacgtaaaa
   104881 cccggctttg tcacgcacga caatgggcgt tttgccctgt tttttcgcca gttttaccgt
   104941 agtggcgatg gtttgcgccg atgtacccgc atgaggaata atctctacca gcggcatttt
   105001 ttccaccgga ctgaagaaat gcaggccaat aacttgctca ggtcgcgcgg cgtgagcggc
   105061 gatatcacca atcggtaaag atgacgtgtt cgaagcaaaa atggtatgag cggcgcaatt
   105121 ttgctccact tccgccacca tctgttgttt taattcgaga ttttcaaaca ccgcttcaat
   105181 aatcagatcg cgatgggcaa agccgcgata gtccgtcgtt ccggagatta atgccagctg
   105241 tttgtcacgt tcgctggctt tgagatgacg acggcgaact ttgccctcca gctgatccca
   105301 actgtacttc agcgcatgat ttatgccctg cgggttgata tctttaattc tgaccggaag
   105361 ccccgcttta caagcagtga cataagcaat accgccgccc atcaagccac cacctaaaat
   105421 tcccacgctg tttaatggcg caggcggtgc atcactaccg ggatctttct tcacgtccgt
   105481 actggcaaaa aagatactac gcaaagcttg cgattgcggc gtcatcgcca gttcgccaaa
   105541 ggcgcgagct tcagcgtcat agccgctgct ggtgccctgc gctaatcccg tttcaacaac
   105601 ctccaggatg cgttctgtcg ccggataatt gccttgagtt ttgtgttctg ttttcttgcc
   105661 gaccattttg aacagcagcg cacgacctaa cggccccgcc agaatacgct cgcgtacagg
   105721 tagagggcgg gaagatgggc gatccttctt cgccagctca acagcggctt ccagcaaaat
   105781 ggagtgcgga acaacgtcat ccaccagccc cagctttaat gcctgtttcg cccgaagttg
   105841 ttttccggtg agaatcatct ctaatgctgt actgacgcct atcagacgcg gtaaacgctg
   105901 ggtgccgcct gaaccgggta acaatccaag ttgtacttca ggcaaaccga gcaccgtttt
   105961 gggatcgtca gtacaaacgc gaccgtggca cgccagcgcc agctccagcc caccgcccag
   106021 gcaagcgcca tgaatagccg cgataaccgg aatgggcaaa gcatgaatct ccgccatcaa
   106081 ctgttggccc tgccgcgcca gtgcttccgc ttcttgcgcc gttttgcagt tgccaatcat
   106141 gttgatgtct gctccggcga taaaattgtc cggtttcgcg gagataaaca ccacgccgcg
   106201 caactctttg ttttcacgga gttgcttaat aatggcgcgc acctgcgagg caaattccgc
   106261 cttcagggta ttcattttct catccggtac gtcgatggtg atgatagcaa tgttgtccag
   106321 acgaacatta agagtaaacg ctgatgccat ttccattatt ccgcctccag aaccattgcc
   106381 gcgccaagcc caccggcagc acaggcggta actaaaccaa atccaccgcc gcgacggcga
   106441 agttcatgca atgtctgggt aatcatccgc gcgccggtcg ccgcgaaggg atgtccgtaa
   106501 gcaatcgaac cgccaagcac gttaaatttg ctatcgtcca cttcgccagt ggcatgtgca
   106561 cgccccagta cttcacgagc aaaacgttca ctacccagca actgaatatt cgccagtgtc
   106621 tgagctgcaa aggcttcgtg catatcgatc aatgtcagtt cgctcatcgt caaaccggca
   106681 cgttccagcg ccagcggtgt tgaccaggct ggaccgagca acatgtcctg ccagacatca
   106741 atcgcagtaa atgcgtagct gcgcagatac cccagcggca ccagccctaa ttctttcgcc
   106801 cgggattcag tcatcaggat caccgctgcc gcgccatcgg tcagcggcgt actgtttgcc
   106861 gccgttaccg ttccgtgttt gcgatcaaac gccgggcgca gctttgcgta atcggcaagc
   106921 gaggaattac cgcgaatatt gttgtcttcg acaagcggtt gtttataagg agggataaag
   106981 gcagtcatca cctcttcttt gagttttccg tctgaccatg cctgagcggc acgctgatgc
   107041 gaacggtgcg ctaatgcatc ttgctgttct cgggtgatgc cgtaggtttt cgccatttgt
   107101 tcggcggtgt cgcccatccg caagccagta gaatattctg ctaccgcagg cggtacgggc
   107161 attaagtcgc gcaaacgcag gcgagagaag agtttcaggc gctggctcat ggttcgagct
   107221 ttgttgacat caaccagcac gtgcgccagt tttttactga cgccaattgg caatacggaa
   107281 gaggaatctg ccccaccggc aatccccgct cggatagttc ccgccatcag gctttctgcg
   107341 acgtttgcaa ccgcctggaa actggtagcg caagcgcggc tgacgctgta agcatcggta
   107401 tgcacattca ttccggtacc gagaacaatt tcacgcgcaa tgttgggggc ttcaggcatt
   107461 tgtacgacct gaccaaagac cagctgttca atcacttcag cggggatctc gctgcgtgcc
   107521 agcagttcgc ctaccaccat cttccctaaa tcaaccgcgg gaatgccatg aaaagccgtc
   107581 gcctggcggg caaaaggcgt acgtaaaccg ctaacaatgg cgatacgatc gccctggcgg
   107641 gtaaccagtg gtaaaacctg acccataaca ctcccctgta aaaaaataaa taaagtggtc
   107701 tgacctgatc atagtattaa ccattttttt acatttagcc aagcggagaa aaaggaaagt
   107761 gggagctatg acacagtgaa gacagaagaa agaaaagaaa acgcccctgc cagcaactga
   107821 caggggcgta cgtacccgag aggaattaac gcagacccag ctggaagatc agcatttcag
   107881 cttcgcaggc aaaagtgaaa tcgatatcca ggcgcacacc gtcagactct tcagtgaaag
   107941 tcggggagat tttgcacggt tcggattcca cgctacggga tttttcagtc agcgccgcca
   108001 gcgtttgttc tgcttctgcg cggtttgcaa acacgcggct gtaagacgcg gtgcagtcgg
   108061 agttgtccat aatggtgcca acatccatac agcagcaaac cggggtttca tcagcactac
   108121 atttactcat cgttgatttc ctctgtatgt gcacccaagg tgccagataa acgttgtgga
   108181 tattttacgc ttccggaaag tgctgctcca gttgttaatt ctgcaaaatc ggataagtga
   108241 ccgaaatcac acttaaaaat gatctaaaac aaaattcacc cgaatccgcg agtgcgcaac
   108301 ctccaaattt tgccagctgg atcgcgtttc ttagatcata tttgaaaaaa gatggaaaca
   108361 tacttgcaac attctagctg gtccgaccta tactctcgcc actggtctga tttctaagat
   108421 gtacctcaga ccctacactt cgcgctcctg ttacagcacg taacatagtt tgtataaaaa
   108481 taaatcattg aggttatggt catgagccag aaaaccctgt ttacaaagtc tgctctcgca
   108541 gtcgcagtgg cacttatctc cacccaggcc tggtcggcag gctttcagtt aaacgaattt
   108601 tcttcctctg gcctgggccg ggcttattca ggggaaggcg caattgccga tgatgcaggt
   108661 aacgttagcc gtaaccccgc attgattacc atgtttgacc gcccgacatt ttctgcgggt
   108721 gcggtttata ttgacccgga tgtgaatatc agcggaacgt ctccgtctgg tcgtagcctg
   108781 caagccgata acatcgcgcc tacggcatgg gttccgaaca tgcactttgt tgcaccgatt
   108841 aacgaccaat ttggttgggg cgcttctatt acctctaact atggtctggc aacagagttt
   108901 aatgatactt atgcaggcgg ctctgtcggg ggtacaactg accttgaaac catgaacctg
   108961 aacttaagcg gtgcgtatcg cttaaataat gcatggagct ttggtcttgg tttcaacgcc
   109021 atctacgctc gcgcgaaaat tgaacgtttc gcaggcgatc tggggcagtt ggttgctggt
   109081 cagattatgc aatctcctgc cgggcaaact cctcaagggc aagcattggc agctaccgcc
   109141 aacggtatcg acagtaatac caaaatcgct catctgaacg gtaaccagtg gggctttggc
   109201 tggaacgccg gaatcctgta tgaactggat aaaaataacc gctatgcact gacctaccgt
   109261 tctgaagtga aaattgactt caaaggtaac tacagcagcg atcttaatcg tgcgtttaat
   109321 aactacggtt tgccaattcc taccgcgaca ggtggcgcaa cgcaatcggg ttatctgacg
   109381 ctgaacctgc ctgaaatgtg ggaagtgtca ggttataacc gtgttgatcc acagtgggcg
   109441 attcactata gcctggctta caccagctgg agtcagttcc agcagctgaa agcgacctca
   109501 accagtggcg acacgctgtt ccagaaacat gaaggcttta aagatgctta ccgcatcgcg
   109561 ttgggtacca cttattacta cgatgataac tggactttcc gtaccggtat cgcctttgat
   109621 gacagcccag ttccggctca gaatcgttct atctccattc cggaccagga ccgtttctgg
   109681 ctgagtgcag gtacgactta cgcatttaat aaagatgctt cagtcgacgt tggtgtttct
   109741 tatatgcacg gtcagagcgt gaaaattaac gaaggcccat accagttcga gtctgaaggt
   109801 aaagcctggc tgttcggtac taactttaac tacgcgttct gataacgcgt tcgcctggat
   109861 aaagtcacct gctatgcagg tgactttaac tcccccactt caccgaaagt agtttcctca
   109921 ttgcttgcct cctgagtttt gcaaacagcc tgatggcagc ttgccctttc aaatcagtaa
   109981 gtggtatcca cagacaaacc tgaagggaaa tgcatatttt caagcgttct gctcgtcctt
   110041 ctcaaagagt ttacttttct gcatttccag gatactcaac ccccctggct attgtgcgct
   110101 tatacactca aattaaagat aggttctaaa taaatgagcg ttttttgata gtctatttca
   110161 ttaggtaata tatatttgta acaaatcaat caacatagaa taaaatcatg ctaccatcta
   110221 tttcaatcaa caataccagc gcagcttacc cagaatccat caatgaaaat aacaatgatg
   110281 aaattaatgg attagtacaa gagttcaaaa acctttttaa tggtaaggaa ggaataagca
   110341 cctgtattaa acatctactt gagcttataa aaaacgccat acgagtaaac gacgatcctt
   110401 atagatttaa tattaataat tcctcagtta cttatattga tattggctcc aatgatacag
   110461 accatattac tattggtatc gacaaccaag aaccaataga attacctgcg aactataaag
   110521 acaaagaact cgtccgtact atcattaatg acaacatagt tgagaagact catgatatca
   110581 ataacaagga aatgatcttc agcgcattaa aagaaatata tgatggagat cctggtttta
   110641 tcttcgataa gatatcacac aaactcagac atacggtaac ggaatttgat gagagcggga
   110701 aaagcgaacc aacggactta tttacctggt acggtaaaga taaaaaaggc gactctctcg
   110761 ctattgtaat taaaaataaa aacggaaatg attacttatc tctcggttac tacgatcagg
   110821 acgactacca cattcaaaga ggaattcgta ttaatggtga tagtctcacc caatattgta
   110881 gtgaaaacgc caggagtgct tcagcgtggt ttgaaagcag taaagctatc atggcagaat
   110941 catttgcaac tggttccgat catcaggttg taaacgagct caacggggaa agactgagag
   111001 aacctaacga agtttttaaa cgtttaggtc gagcaataag atataatttt caagtggacg
   111061 atgcaaaatt tagacgcgat aatgtaaaag aaataatttc tactttattc gctaacaaag
   111121 ttgacgttga ccatcctgaa aataaataca aagactttaa ggatctcgaa gataaagttg
   111181 aaaaaagact tcaaaattgc caggcgaaat atcaaaatga aattaatcaa ctatctgcac
   111241 taggtgttaa ttttgatgat atttaaaatt atttttccag ttttttgatt tttcaacgcc
   111301 tgaatatcgg aaatagtcag tgacaaac
//
LOCUS       DAJTXY010000003        86242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTXY010000003 DAJTXY010000000
VERSION     DAJTXY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 86242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.003"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..1582)
                     /locus_tag="OO566_000241"
     CDS             complement(<1..1582)
                     /locus_tag="OO566_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6603664.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDKNEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGT"
     gene            complement(1566..1922)
                     /locus_tag="OO566_000242"
     CDS             complement(1566..1922)
                     /locus_tag="OO566_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6603665.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2045..2227)
                     /locus_tag="OO566_000243"
     CDS             complement(2045..2227)
                     /locus_tag="OO566_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603666.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2211..2651)
                     /locus_tag="OO566_000244"
     CDS             complement(2211..2651)
                     /locus_tag="OO566_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6603667.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2651..2947)
                     /locus_tag="OO566_000245"
     CDS             complement(2651..2947)
                     /locus_tag="OO566_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6603668.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2944..3282)
                     /locus_tag="OO566_000246"
     CDS             complement(2944..3282)
                     /locus_tag="OO566_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6603669.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3279..4490)
                     /locus_tag="OO566_000247"
     CDS             complement(3279..4490)
                     /locus_tag="OO566_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6603670.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4492..5064)
                     /locus_tag="OO566_000248"
     CDS             complement(4492..5064)
                     /locus_tag="OO566_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6603671.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5104..6261)
                     /locus_tag="OO566_000249"
     CDS             complement(5104..6261)
                     /locus_tag="OO566_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6603672.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6561..6785)
                     /locus_tag="OO566_000250"
     CDS             complement(6561..6785)
                     /locus_tag="OO566_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603673.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(6927..7088)
                     /locus_tag="OO566_000251"
     CDS             complement(6927..7088)
                     /locus_tag="OO566_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6603674.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(7098..7403)
                     /locus_tag="OO566_000252"
     CDS             complement(7098..7403)
                     /locus_tag="OO566_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603675.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7400..7678)
                     /locus_tag="OO566_000253"
     CDS             complement(7400..7678)
                     /locus_tag="OO566_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603676.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7958..9706)
                     /locus_tag="OO566_000254"
     CDS             complement(7958..9706)
                     /locus_tag="OO566_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6603677.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9703..10002)
                     /locus_tag="OO566_000255"
     CDS             complement(9703..10002)
                     /locus_tag="OO566_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6603678.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(10008..10241)
                     /locus_tag="OO566_000256"
     CDS             complement(10008..10241)
                     /locus_tag="OO566_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603679.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(10243..10463)
                     /locus_tag="OO566_000257"
                     /pseudo
     CDS             complement(10243..10463)
                     /locus_tag="OO566_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10456..10677)
                     /locus_tag="OO566_000258"
     CDS             complement(10456..10677)
                     /locus_tag="OO566_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603680.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10667..10867)
                     /locus_tag="OO566_000259"
     CDS             complement(10667..10867)
                     /locus_tag="OO566_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6603681.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10876..11622)
                     /locus_tag="OO566_000260"
     CDS             complement(10876..11622)
                     /locus_tag="OO566_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603682.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11680..11868)
                     /locus_tag="OO566_000261"
     CDS             complement(11680..11868)
                     /locus_tag="OO566_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6603683.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(12263..13492)
                     /locus_tag="OO566_000262"
     CDS             complement(12263..13492)
                     /locus_tag="OO566_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6603684.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13741..14862
                     /gene="roxA"
                     /locus_tag="OO566_000263"
                     /gene_synonym="ycfD"
     CDS             13741..14862
                     /gene="roxA"
                     /locus_tag="OO566_000263"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6603685.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14911..16137)
                     /gene="pepT"
                     /locus_tag="OO566_000264"
     CDS             complement(14911..16137)
                     /gene="pepT"
                     /locus_tag="OO566_000264"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6603686.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16387..17523
                     /gene="potA"
                     /locus_tag="OO566_000265"
     CDS             16387..17523
                     /gene="potA"
                     /locus_tag="OO566_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6603687.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17507..18364
                     /gene="potB"
                     /locus_tag="OO566_000266"
     CDS             17507..18364
                     /gene="potB"
                     /locus_tag="OO566_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6603688.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            18361..19155
                     /gene="potC"
                     /locus_tag="OO566_000267"
     CDS             18361..19155
                     /gene="potC"
                     /locus_tag="OO566_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6603689.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            19152..20198
                     /gene="potD"
                     /locus_tag="OO566_000268"
     CDS             19152..20198
                     /gene="potD"
                     /locus_tag="OO566_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6603690.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(20354..21175)
                     /gene="cobB"
                     /locus_tag="OO566_000269"
     CDS             complement(20354..21175)
                     /gene="cobB"
                     /locus_tag="OO566_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6603691.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCHFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(21191..22102)
                     /gene="nagK"
                     /locus_tag="OO566_000270"
     CDS             complement(21191..22102)
                     /gene="nagK"
                     /locus_tag="OO566_000270"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6603692.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(22131..23375)
                     /gene="lolE"
                     /locus_tag="OO566_000271"
     CDS             complement(22131..23375)
                     /gene="lolE"
                     /locus_tag="OO566_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6603693.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23375..24076)
                     /gene="lolD"
                     /locus_tag="OO566_000272"
     CDS             complement(23375..24076)
                     /gene="lolD"
                     /locus_tag="OO566_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6603694.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(24069..25268)
                     /gene="lolC"
                     /locus_tag="OO566_000273"
     CDS             complement(24069..25268)
                     /gene="lolC"
                     /locus_tag="OO566_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6603695.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25530..26576
                     /locus_tag="OO566_000274"
     CDS             25530..26576
                     /locus_tag="OO566_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6603696.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26704..30150
                     /gene="mfd"
                     /locus_tag="OO566_000275"
     CDS             26704..30150
                     /gene="mfd"
                     /locus_tag="OO566_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6603697.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            30293..31255
                     /gene="ldtC"
                     /locus_tag="OO566_000276"
                     /pseudo
     CDS             30293..31255
                     /gene="ldtC"
                     /locus_tag="OO566_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(31338..31595)
                     /gene="bhsA"
                     /locus_tag="OO566_000277"
     CDS             complement(31338..31595)
                     /gene="bhsA"
                     /locus_tag="OO566_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR6603698.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31836..32468
                     /gene="comR"
                     /locus_tag="OO566_000278"
     CDS             31836..32468
                     /gene="comR"
                     /locus_tag="OO566_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6603699.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32530..33069)
                     /locus_tag="OO566_000279"
     CDS             complement(32530..33069)
                     /locus_tag="OO566_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6603700.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(33296..34600)
                     /gene="ndh"
                     /locus_tag="OO566_000280"
     CDS             complement(33296..34600)
                     /gene="ndh"
                     /locus_tag="OO566_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6603701.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(35001..35543)
                     /locus_tag="OO566_000281"
     CDS             complement(35001..35543)
                     /locus_tag="OO566_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6603702.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35566..36591)
                     /gene="nagZ"
                     /locus_tag="OO566_000282"
     CDS             complement(35566..36591)
                     /gene="nagZ"
                     /locus_tag="OO566_000282"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6603703.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36602..37426)
                     /gene="thiK"
                     /locus_tag="OO566_000283"
     CDS             complement(36602..37426)
                     /gene="thiK"
                     /locus_tag="OO566_000283"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6603704.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37407..38048)
                     /gene="lpoB"
                     /locus_tag="OO566_000284"
     CDS             complement(37407..38048)
                     /gene="lpoB"
                     /locus_tag="OO566_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6603705.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(38063..38440)
                     /locus_tag="OO566_000285"
     CDS             complement(38063..38440)
                     /locus_tag="OO566_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6603706.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38443..38802)
                     /gene="hinT"
                     /locus_tag="OO566_000286"
     CDS             complement(38443..38802)
                     /gene="hinT"
                     /locus_tag="OO566_000286"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6603707.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            39136..39960
                     /locus_tag="OO566_000287"
     CDS             39136..39960
                     /locus_tag="OO566_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6603708.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPT"
     gene            39964..41325
                     /gene="fhuE"
                     /locus_tag="OO566_000288"
                     /pseudo
     CDS             39964..41325
                     /gene="fhuE"
                     /locus_tag="OO566_000288"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41385..42818)
                     /gene="ptsG"
                     /locus_tag="OO566_000289"
     CDS             complement(41385..42818)
                     /gene="ptsG"
                     /locus_tag="OO566_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6603709.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(43113..43910)
                     /locus_tag="OO566_000290"
     CDS             complement(43113..43910)
                     /locus_tag="OO566_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6603710.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43921..44925)
                     /gene="holB"
                     /locus_tag="OO566_000291"
     CDS             complement(43921..44925)
                     /gene="holB"
                     /locus_tag="OO566_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6603711.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAALVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44922..45563)
                     /locus_tag="OO566_000292"
     CDS             complement(44922..45563)
                     /locus_tag="OO566_000292"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6603712.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45553..46575)
                     /gene="yceG"
                     /locus_tag="OO566_000293"
     CDS             complement(45553..46575)
                     /gene="yceG"
                     /locus_tag="OO566_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6603713.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46578..47387)
                     /gene="pabC"
                     /locus_tag="OO566_000294"
     CDS             complement(46578..47387)
                     /gene="pabC"
                     /locus_tag="OO566_000294"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6603714.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47507..48748)
                     /gene="fabF"
                     /locus_tag="OO566_000295"
     CDS             complement(47507..48748)
                     /gene="fabF"
                     /locus_tag="OO566_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6603715.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48835..49071)
                     /gene="acpP"
                     /locus_tag="OO566_000296"
     CDS             complement(48835..49071)
                     /gene="acpP"
                     /locus_tag="OO566_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6603716.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(49282..50016)
                     /gene="fabG"
                     /locus_tag="OO566_000297"
     CDS             complement(49282..50016)
                     /gene="fabG"
                     /locus_tag="OO566_000297"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6603717.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGSQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(50029..50958)
                     /gene="fabD"
                     /locus_tag="OO566_000298"
     CDS             complement(50029..50958)
                     /gene="fabD"
                     /locus_tag="OO566_000298"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6603718.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50974..51927)
                     /gene="fabH"
                     /locus_tag="OO566_000299"
     CDS             complement(50974..51927)
                     /gene="fabH"
                     /locus_tag="OO566_000299"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6603719.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(51995..53065)
                     /gene="plsX"
                     /locus_tag="OO566_000300"
     CDS             complement(51995..53065)
                     /gene="plsX"
                     /locus_tag="OO566_000300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6603720.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(53146..53319)
                     /gene="rpmF"
                     /locus_tag="OO566_000301"
     CDS             complement(53146..53319)
                     /gene="rpmF"
                     /locus_tag="OO566_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6603721.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53371..53892)
                     /gene="yceD"
                     /locus_tag="OO566_000302"
     CDS             complement(53371..53892)
                     /gene="yceD"
                     /locus_tag="OO566_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6603722.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            54091..54675
                     /gene="yceF"
                     /locus_tag="OO566_000303"
     CDS             54091..54675
                     /gene="yceF"
                     /locus_tag="OO566_000303"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6603723.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54787..55746)
                     /gene="rluC"
                     /locus_tag="OO566_000304"
     CDS             complement(54787..55746)
                     /gene="rluC"
                     /locus_tag="OO566_000304"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6603724.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            56319..59504
                     /gene="rne"
                     /locus_tag="OO566_000305"
     CDS             56319..59504
                     /gene="rne"
                     /locus_tag="OO566_000305"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6603725.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59701..60654)
                     /gene="flgL"
                     /locus_tag="OO566_000306"
     CDS             complement(59701..60654)
                     /gene="flgL"
                     /locus_tag="OO566_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6603726.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60666..62308)
                     /gene="flgK"
                     /locus_tag="OO566_000307"
                     /pseudo
     CDS             complement(60666..62308)
                     /gene="flgK"
                     /locus_tag="OO566_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62374..63315)
                     /gene="flgJ"
                     /locus_tag="OO566_000308"
     CDS             complement(62374..63315)
                     /gene="flgJ"
                     /locus_tag="OO566_000308"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6603727.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(63315..64412)
                     /gene="flgI"
                     /locus_tag="OO566_000309"
     CDS             complement(63315..64412)
                     /gene="flgI"
                     /locus_tag="OO566_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6603728.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64424..65122)
                     /gene="flgH"
                     /locus_tag="OO566_000310"
     CDS             complement(64424..65122)
                     /gene="flgH"
                     /locus_tag="OO566_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6603729.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(65175..65957)
                     /gene="flgG"
                     /locus_tag="OO566_000311"
     CDS             complement(65175..65957)
                     /gene="flgG"
                     /locus_tag="OO566_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6603730.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(66095..66846)
                     /gene="flgF"
                     /locus_tag="OO566_000312"
                     /pseudo
     CDS             complement(66095..66846)
                     /gene="flgF"
                     /locus_tag="OO566_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(66866..68065)
                     /gene="flgE"
                     /locus_tag="OO566_000313"
     CDS             complement(66866..68065)
                     /gene="flgE"
                     /locus_tag="OO566_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6603731.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTCAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIVATT
                     QNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQ
                     SSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQIL
                     NTRVNLR"
     gene            complement(68090..68785)
                     /gene="flgD"
                     /locus_tag="OO566_000314"
                     /pseudo
     CDS             complement(68090..68785)
                     /gene="flgD"
                     /locus_tag="OO566_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(68797..69200)
                     /gene="flgC"
                     /locus_tag="OO566_000315"
                     /pseudo
     CDS             complement(68797..69200)
                     /gene="flgC"
                     /locus_tag="OO566_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(69204..69377)
                     /locus_tag="OO566_000316"
     CDS             complement(69204..69377)
                     /locus_tag="OO566_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HCR6603732.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69532..70191
                     /gene="flgA"
                     /locus_tag="OO566_000317"
     CDS             69532..70191
                     /gene="flgA"
                     /locus_tag="OO566_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6603733.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            70267..70543
                     /gene="flgM"
                     /locus_tag="OO566_000318"
                     /pseudo
     CDS             70267..70543
                     /gene="flgM"
                     /locus_tag="OO566_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
     gene            70548..70964
                     /gene="flgN"
                     /locus_tag="OO566_000319"
     CDS             70548..70964
                     /gene="flgN"
                     /locus_tag="OO566_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6603734.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(71004..72539)
                     /gene="murJ"
                     /locus_tag="OO566_000320"
     CDS             complement(71004..72539)
                     /gene="murJ"
                     /locus_tag="OO566_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6603735.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSHLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72706..73572)
                     /locus_tag="OO566_000321"
     CDS             complement(72706..73572)
                     /locus_tag="OO566_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6603736.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(73574..74221)
                     /locus_tag="OO566_000322"
     CDS             complement(73574..74221)
                     /locus_tag="OO566_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6603737.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(74232..74816)
                     /gene="rimJ"
                     /locus_tag="OO566_000323"
     CDS             complement(74232..74816)
                     /gene="rimJ"
                     /locus_tag="OO566_000323"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6603738.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            75052..76260
                     /gene="mdtH"
                     /locus_tag="OO566_000324"
     CDS             75052..76260
                     /gene="mdtH"
                     /locus_tag="OO566_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6603739.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEYR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            76324..76971
                     /gene="grxB"
                     /locus_tag="OO566_000325"
     CDS             76324..76971
                     /gene="grxB"
                     /locus_tag="OO566_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6603740.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYHDNMAKQTQINLLSSMAI"
     gene            77105..77665
                     /locus_tag="OO566_000326"
     CDS             77105..77665
                     /locus_tag="OO566_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6603741.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77771..78817
                     /gene="pyrC"
                     /locus_tag="OO566_000327"
     CDS             77771..78817
                     /gene="pyrC"
                     /locus_tag="OO566_000327"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6603742.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78891..79136
                     /gene="dinI"
                     /locus_tag="OO566_000328"
     CDS             78891..79136
                     /gene="dinI"
                     /locus_tag="OO566_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6603743.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            79426..79680
                     /gene="bssS"
                     /locus_tag="OO566_000329"
     CDS             79426..79680
                     /gene="bssS"
                     /locus_tag="OO566_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6603744.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79795..80913
                     /gene="solA"
                     /locus_tag="OO566_000330"
     CDS             79795..80913
                     /gene="solA"
                     /locus_tag="OO566_000330"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6603745.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80961..81074
                     /locus_tag="OO566_000331"
     CDS             80961..81074
                     /locus_tag="OO566_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6603746.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            81335..81901
                     /locus_tag="OO566_000332"
     CDS             81335..81901
                     /locus_tag="OO566_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6603747.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81905..82480
                     /locus_tag="OO566_000333"
     CDS             81905..82480
                     /locus_tag="OO566_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6603748.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82522..83574)
                     /locus_tag="OO566_000334"
     CDS             complement(82522..83574)
                     /locus_tag="OO566_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6603749.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83799..84719
                     /gene="lpxL"
                     /locus_tag="OO566_000335"
     CDS             83799..84719
                     /gene="lpxL"
                     /locus_tag="OO566_000335"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6603750.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84891..85088
                     /locus_tag="OO566_000336"
     CDS             84891..85088
                     /locus_tag="OO566_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6603751.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            85112..>86242
                     /locus_tag="OO566_000337"
     CDS             85112..>86242
                     /locus_tag="OO566_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6603752.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT        20697 a        22920 c        21231 g        21394 t
ORIGIN      
        1 atgtaccaaa cgccatcagc gccgccacgc tcgggtctat cttgttcggt gattttttct
       61 tgttcggctt gatattggcg ttcgcgtcac tctgcatcac gacgttactc atcgaccagg
      121 acaacaccgg atcgccacga tgcacaatca ccctgcggtt aacaaaaact tcgaacgatt
      181 tcgccgccgg actgaatctg aggtaggttt gcgggaacgg ctccacctca aaaccagccc
      241 cctgtaattg cgtcctgaga tgcgtggcgt tccacgtatc aaagcccacc agcctgatat
      301 taaatttctc cgcatcctgc atgatgtcat ctctgatccg gtcgtaatca atgcagtcac
      361 ctggcgttgt gcgtatccag cccgctttag cccactggcg atagacagcg cggtttttat
      421 tggcggggtt ctgtagctgg aactccggca gataatgacg ggaaatcagc ataatggttt
      481 taccgaccgg aaaggcatag catacgctgg aaatatcgct ggttgatgat aagtccagcc
      541 ccgcgtaaca ctcctgaccg tgtaaatctt cctccgtgaa cgttccggca cactcagccc
      601 atgcaccatt acccatccac ggggtagtcc cctgacacca gatattaaat cgctttgtca
      661 tcatctccac ccactgcgac ggaatacccc gcgctttctg gatggttgag gccagttttt
      721 cacgatccac ggaaacatca atattagggt tcgcctttat ccacatcgcc ggatcatcaa
      781 cctcgttttt gtcgtccagt tcgtaaatca gtacaaaaat tgaatcgttg acctcttcgc
      841 cgtccaggat ctggcagcaa taatcatagt gctgtttaca ggctgaaacg acgttactgc
      901 ccgatgtggt aatggcaaat aacagccctt cgggacgtgc gcccatcccc agctcaagcg
      961 cggaataaac gccgttatcg gggtgtaggt ggtattcgtc cacgatggaa agactggggt
     1021 ttgtcccctc gatggttgcc gcttttgctg ccagcggctt taacaggctg ttgcttttcg
     1081 ggtgtatcac cttatgcgcc tgaatattca cccgcctgcg taacggtcgg gataaaaggc
     1141 acatctgacg cgcatcatca aacacgatcc gcgcctggtc acggctcacc gctgcggtgt
     1201 agatatcctg ctgaccgttc tccataatca gaaaccagtt agccagaatc gcggcggtcg
     1261 tggatttcgc atttttgcgc ggcacttcga taaaggcgct cgtgtatttg cgccgtccgg
     1321 tggccttaac cttaaagccg aggatgcacg caaaggcgaa ctgctgccac ggctccagct
     1381 caatgggtct gccacgcatc ggtcctttta cgtgcgggca caccctggaa aaggcaataa
     1441 accgctccac aacctcacga tcgaacatgt aaagggggct tttaaggtcc gaaaagtacc
     1501 gtttaacagc ctgttttaac cgtttacagg ccggaatttt gcccgttttt acgtcttctg
     1561 cgtacttatt ccaggcggtc aagctcgtcc tcttcttctg tttccggtgg atttttacgg
     1621 cggcttatcg ggtcaaaacc gagcaaggag gccattttta tcatcactct ttcagcgtcg
     1681 gattttgcgc ttaatgcggg gtttctgctc tcgccgccct gactgttaac aatgctgaac
     1741 ccgcgcgccg caaggtctgc aacggctttc cggtaaatgg agtagttgac gcaatacagt
     1801 tccagattgc tccagtcggc gggggtcagg tctccccgtt ccgcaagctg ccgcgatttt
     1861 tcccgccact gcttcacggc gatatcatcc aggtaggcgg gggctttcgg tggtcttgcc
     1921 atgcttattt ttcgtcagat tatttttcaa aaaattcccg tgcataaaaa tttggggagg
     1981 cgttcggtgc ccggcggggt cgggtttgtc ctgaaaacgc cccccacccc gtcatacagc
     2041 ctcatcagcg attgcggaaa cattccataa cctcgcggtc acggtcggtt aatcgcttcg
     2101 ctgtgatgcg ttctgcgcgt cctgacgctt tatctttatg ccctgtttcc tgtgtcttcc
     2161 atgcgtcacg ctgccttata agtccacgga taaggcggtt ttgttcccgc tcattcatca
     2221 gcgtcataca tccagttatt gcggttagca gcccgttctt cctcttcacg aaatccacct
     2281 gcggcacgct tgctttttgt tgccggatca agccatttcg ttttctggtt atgacacgcc
     2341 tggcacaatg gttgatggtt ccattcgggc cagaagagaa catcatcacc gccattaatc
     2401 gggataatgt gatccaccac cacggcgggc gtatatatcc ccttctcaag gcatcgcacg
     2461 cataacgggt ttttactcag atacatggcg cggtatttgt cccactgtct ggaatatcca
     2521 cgcgcgcggc ggtgtcctcg tctggcatcc tctgcacgcc atgccgcccg cctgtgctcc
     2581 tcacacttgc cggatttaac gcgcctgtta cagcctggtt ctgtgcatcg tcttaatggt
     2641 tgccacggca tcagtacacc cccacatcac ggtaagccgt ccagagtgcg cctatcgtca
     2701 tgggtacgcg tgtttttgcg ttatccgcga caatctggcg attttcatac aggtgagcga
     2761 taaacatcat gcagccaatc tttatggctg gcgtgaacgc cagcccgtca tcaaagcgcc
     2821 tgcctatgtg tatctggcac gcctcaagcg atgcggcaat gtatccgctg attaactggt
     2881 cttcatcgtc gccatcgatg cggcaatgga gtttcacttc ttccagggtg ataagttctt
     2941 ctgtcatttt tccgcgccct cacgacaaag aatttcaagg cgtgtcctgg cggcatccgg
     3001 cagcggctgc ccgatgatat tcagtacacg ccccgccagc ggccccgtac tgacctttat
     3061 ccggctggtg gcgttgatgt ccttccggta ccgtatccag atccttacgg taccaatcgc
     3121 cagctctgcg cccgatgaaa tggcctcctt gctgctgacc atgttcacgc ttgcccagag
     3181 tgtgtgaccg tcctcccacg tttcgagtat ttcgccggtt atatccctgg tctgtttcag
     3241 ggtctgaatc gttatcctgt cacgcagtcg ccctatgttc attccggttt ttctccctcg
     3301 ctgattttta cttcctgttt ccatgcctgg ctgaactcat caccaccatc acgcggtgac
     3361 agcccttcac gttcgcgggc ttcgttcgga cacataacgc cggattttat gccgcgctca
     3421 taggtggcaa agcgttcgcc aggtgtggcc cgtaacaggt ccgcgctgtc aaactccacc
     3481 tgataccgga taccaggtac aggcgaggcc accagcaagg cattttttat ctgctgctca
     3541 aagttcgcca gccacgggcg catcgtcatg gtgagaaatg cgcggcttgc ctcgctgaag
     3601 ttgctgtagg tgctgttgct gtattcctgc aaaaaaatcg gcgagacgtt gaacattcgg
     3661 gcgatgtctt caatggtgaa gcgacgggag gccagccatt cggcatcctg attactcatg
     3721 cccagctgct gatagctcat ccccccttca aggatgggcg tttttccggc gtttctggcc
     3781 cctttgtagc gttccagtgc ggctaatgcc tgtttgccct tcacgccgtc cagccattcg
     3841 cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc gccgtggcgt
     3901 tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga gcgccccata
     3961 aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata gttgcgggtg
     4021 tttccggtgt aggcgtcagt gatgcagtag cgccagttat gatcacctgt ctgctccacg
     4081 ttcaccgact gcggcgggta aggatgtaaa gccgccggaa aaccatcacg cccccactga
     4141 ataaccgcat aagcattacc acttaacagg cagtggcgga tcatcattcg cttaaactgg
     4201 tagggtgtct gccacgcgtt cgggcgctcg ttgaggatgt gatcgaccgg atgagaatca
     4261 agccattcgc gggcctcctt ccccttctca ttgcgtacca ggtacaggta acacggcatg
     4321 gtagccaccg cctcagagat gaccgtgacg gcgttcatga cggcggagag tgattccgct
     4381 gtcccagatg atacgtactc acccgccccc gtgttcgatg tgccggccag cgccataaat
     4441 tcatcaagcg tcatgctgcg ctgctctttt tttcgtctga aaggccacat atcacacccc
     4501 cgctaaatcc gcccaccagc ggcgattatc ctcacgcggc attttttcgg ggtgctgctc
     4561 atacagggaa cggcgggcca gctccacgcc ggaatcgggg taagccggta ccgatgtaac
     4621 ggtaatttcg tacagttccg ccaccagcac ggtgcgcacg catggatctg ttgtggtatc
     4681 ccatacatcc ttacgggagc gaaagctaaa gctcatcccc gagatgtccc cacgcttaac
     4741 cagttcgata acgtcgcgcc ctgtactggt atccggtggg gtcagttcaa agcgtaaccc
     4801 tgtttcgtcc tcttccagtt tcagcgtgcc ggaacgggtg cgccccagta acatgctgtg
     4861 gtcatgctca tacaggccgc gaacgtcatt acccgccgca agccattcag taaacgcccc
     4921 ccgctggaat ttttcgtaaa actcacccca taacagttct gaaagcttat cccagcgaac
     4981 aacgtagccc gtcagcgtac cggctccgct ggtggtgatt tccgatgacc ggatttccat
     5041 actcttcata attttttcac ccataaaaca ctaaaggggc tttttagccc cttctgtatg
     5101 ctgttaatcg tcgtcttccg gcagttccag aatcttgatc gcgttcgaat ccaccacgcc
     5161 accgcctaaa tatttttgcg tgaaaatttt gatgaatcct ggttctgtca ggttgtccgg
     5221 tctggtgcga acacctgttt cgtgatcaac aatgtagtaa ccgcgtttga agtcacccag
     5281 ggcaataacg ttatcaggca taaactccag atattcgacc ggaaggccca gcaacgtatc
     5341 aggatcaccc gcctgtaaac ggtcgcgcca gatataatca ccgttcgcgt tcttcacctt
     5401 ctgaagtttt gccgccgtcg tggagttaac cacccagacc gcgtttttgc ggtatttttt
     5461 acgtaatgca aatttcaggt cgatcagcgg gtccgcagat gtccacgcca gagattcgga
     5521 aggtttaatt acctgcaacg taccaaaatc acgctctttg tcgttcttct ctgcacgggg
     5581 tacggataaa aaaccttttg cttttttgtc accgtcgccc acaaccagat cgctttcttc
     5641 ggtttccgtg aaggtgtcgc caatctcacc cgtcagccat gaaaggatgt ccacatcgga
     5701 aaaatccacg atttcctgtg tggtgcgcgg gtacgcatag accggataca gcttaatgct
     5761 cacctcgtta atctgcgggg tgctggtctg ttcgcgtgcc ttaccctctt caccgtggtt
     5821 aacggtcgca ccgccagcgg aaacaagctg cttaaactcg ttgctgctga ttttcttcac
     5881 ggtacagatg cggcgcatag tggattcatc cgccagcatt cgcatgattt cggtgttcag
     5941 ttcagggata acggtataac caccatcagc gggaacgcct gtacttaatg cgcgggtttc
     6001 accggtcaga atgtagttgc gtagttctgc ggggtcagtg gtctggctgc ttttacctgg
     6061 cttgctgcgc tcttcgtctg caatggcttc aaggcgggaa atgtctttat cgagggattc
     6121 agctttagcg cgtaattcgt caaattttgc gccctcagca tcgttaagac tgcggttttc
     6181 tttttcagcg ttctccagca tgtcgcgcat ctgattttta atggcggttt tctgctggcg
     6241 taattcgatt attctctgca taaaaaagtc ctggggttaa gtaaggaact ccaggacgcg
     6301 gcaaacactc aaccgttttt cataaggaaa tcagtaatcg caccgatcgt tttcccgcct
     6361 ggtaatgaat attggcgagc acattaacag gcgggaaagt ggccccagcg tcctggcacc
     6421 acgggcgaga ataatcatga ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa
     6481 cgtggaacaa ccacgaacaa ataatttaca aaaaataaca aaaagccgga ttgctccggc
     6541 tgtatgtgct gtatgggtgc tcagtctctg atccctttca gaaattctat caatgcatct
     6601 gtctgttcag gatttactgc cagcatttca ccggatagcg cgcaacgaac aaaaccatga
     6661 tgatcctttt caatcagcgc ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc
     6721 gtctccatgt tgtcagcatg gtatttatca cgctgtttaa gtatctcatc aaatttcatc
     6781 ggcattgcgt tttcctctgt tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg
     6841 acgaaaagcc agtagtgctc cataccgttt caaatgacgc aaaaaaagcc ggattgctcc
     6901 ggcttcgtgg tgtggttctg atattcctac tctgctaatt tgctcagccc catgtctgcg
     6961 taagtgcgat ttactgcatt tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg
     7021 cccttgctgc tctgttttac cagcgggtcg cccaccagcc gcccgtaagc tgcaatttgt
     7081 gctgtcatat tgccacctca gtgaagcgat ccggcattgt tctttttcat ctcttccatc
     7141 attttttcag tgagcattac gcactgaaac cccatgtttt tgatgtctac gggtttcatg
     7201 cctttgtcag tggatatcac aggcagaata tcctgataca catcgcggtc agggtaacta
     7261 agcacacaaa gcgcccatga tgccacgggt gaggatcctt cgtctgtttc aacccaccag
     7321 cctggtgtag ctgcgcttat ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt
     7381 tcaccaaatt ttggttcgct catgattcca cctctccggt tttaacgttg attgttgtta
     7441 cctgttccgc ttcggcaatc tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg
     7501 tcatgaatgc gcttatcagt tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt
     7561 ctatcgttct gattgccacc tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg
     7621 gttctgttct gtctctgcgt tttttgagtg atttattaaa tttccctgaa gtgtgcatat
     7681 ttatttttac cccctcgttt aaaaagtttt gagttgtgcc tccccttgtc taccttagtg
     7741 gccctcatgc cagtaatggc gctgctttca gcggggtaga gtgcttttat ccactatcta
     7801 ccccgtgtct acctccctgt ctgattcagg taaaatcagg tagagagggt agatagtggg
     7861 tagacagtaa aaaaaggcta cctacctaac ttaatgcact gaattaaatg tatttttctt
     7921 tactcaggta gatagggtag acagcaatta caaaaaatta taaaaacgcg tcgcactcgt
     7981 ctgttgttat tgcgttagtc tgcgttactc ccttaacttt ccgcgtaata tattcatgtc
     8041 cgtaaacttt cgcggctggc ttcatagcct tgccaaagtc atttacgttt agcggtttgc
     8101 tcctgcctgc gtacgccata aacgccagat agacgcggta aaggctgttt ctggtcgtgt
     8161 acttcactga atcgccaccg ccgcccatca tcaggccgcg tgcttcctcc agaaaattca
     8221 ggaactggca aaactcaata accggatccg tctgttgctt tattgccaga gcttcatcac
     8281 cgtcacgctg ttccagtagt aaagcccgtg ctttctcaga gtcggtaaag ttcgccagca
     8341 atcggcggat aatgacaggg atttcagccg caatcttttc cggtagctcc ctgtcttttt
     8401 cggcctcact aacgatattg tcgaaacgga aaatcacgcg acgacgtgcc acacctccgg
     8461 cccgttcggt gaatatcatc gggttattgt tggtcgccag caccaccgcc ctgattacag
     8521 ccgtgaaacg cttttcgtat ttcgggttaa tttccacggg gtcaccgccc gtgattttct
     8581 tgatgcccgt tccttcgcct gtatatttcg gctggtcagc caggacgata agacgactcc
     8641 cgacaacctg cggacgtcca ccagcatcat caagcgatgt catttcagcg cttaccgtgt
     8701 tctgtttccc tgccagaagg ctggctatgt gtgtgaatgt acttttaccg ctcccgccgt
     8761 ctccggtggc ctcaataaac atctgccagt cgtaccggtt cgccataatc atgtacagcg
     8821 cggcacatat acgcatcatc ttgcgctggt cttttccggc tgcgtgctca agccatttat
     8881 gaaagtttag cgcgttatcg cggatgttct cccctggtgc tggtggcgtg tactcaatgc
     8941 cgttgtgcgt ggtgatccag ttctccggcg tgtgcgggga aaattccccc gttttcaggt
     9001 caagcgcacc attggcgaac ggcagcaaat cgccggacgg ctcgcccatt ggttcggcaa
     9061 taacttttaa cgcttccacg gcgttattga ttacgcgctt gctgaaagtg gccctgtgct
     9121 ctgaatagat cgccaccatt tcgcggctaa gttccattgt gctgaccgga caccataccc
     9181 cgccgcgcca tacgtgaacg atttcacttt cctgatgcac acaaacgcca tcaaagcgct
     9241 cggcaagcag ctgcgcgcgc tcactgtctg ccatttgtgc cagttgcgta tttttctgct
     9301 ccggcatggt aagccctgcg gcaatatttt cacgctcagc attcagatag cgccgccagt
     9361 tttcacactt ctggacgtgc attccttcgg ggtaaaaatt tgcgtcctga atatctgccg
     9421 ccgccagctt ctgaccaatc tttttggtct ccaccaaatc cagttctccg gcctggtaca
     9481 gccttacgcg tttttttcca tccggaacaa ttttcagtgc atcaagttcg gcaagctgat
     9541 ttggcccaag ccacacaggc ggcacattat cgccggatgc ggggccatcc tgctcctgcc
     9601 attgtttcgc gtgcgaccag gcatcactac ccgcaaagat aattacctct gtgtctttgt
     9661 gttttattcc gcgtggctgt ttttttacgt tcggtgccag tttcattttt tacccctgaa
     9721 tacgttaagc atctttttta tttcctgaat attggcgcgt gctttctccc tgctggttgg
     9781 tttactgcgg ggcgctgcct gtaccagcgt gaaatcacgc cggaattgat aaacaggcat
     9841 cacgcaatca tattcgtaac cttcacggcg gtaggtgatg cgccgttctg ccacgccttt
     9901 aatcgttacc atgccgccgt actggtcgcg gtaaatatca ccgcgcgtaa atttagggtg
     9961 agtgttgcca ctggcagtta agccagaata tttaagtttc attattttta ttctccggtg
    10021 tgctgttctt tatatctgtc gtgcaataga tctatttctt gcagttccat tattacaggc
    10081 tcaagaagcg ttattaatgc cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg
    10141 ccaagtgttt caagccatat gcgcaatatt tccagcatgt tttcactgtg agaaagtgca
    10201 agaaatgcgc ggtctattgt ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt
    10261 ttttcttaaa ataacctctg tcacgaaatc agcataatcg gcggcgatat tcagtacatc
    10321 aagcccgttg atttatattc tctcgtggaa agtaagaaaa aagccgctct aataagttct
    10381 ggcattgacg aaagcgcatc agccgcatca tcaggaacgc cggaaaattc ctgtttcagg
    10441 gaattaaaac gatcatcacg cataaccccc cccattttca caatcggcaa caagaatatt
    10501 tttagcgtca ttcagtgacc gcgttgcgat gtatcggata cattccaggg cgaactgtgt
    10561 ccggtctttc tgttcttctc cctgcgcaag ctctgccgtg cattcaatat caataagcgc
    10621 gtgcatcagc gtagtgagtg cggcggctgc tgcgttcggt gtggttttat tgtgcatagg
    10681 tttccccctg acgaatacgg gcggcgaata cagcaacaca accggacggg caacggctac
    10741 gcgcttcgcg ttccgtccaa gcggttacgt ggatgatttg ggattctccg gtactcagtg
    10801 ccagaaaacg ccacacaaag gccgtttgtg tgtgtacaag gtgtggtata tgatttacgg
    10861 caaccataac ggctcctagt ttacgttgtt ggttagaagc cccgttactg ctcctacagt
    10921 gcggggcttt gctttttgtg ttgattgtgt gtaatgtgtc attacacata aatacattac
    10981 atgaggtgta attaacgtgt caacacacaa aaacgaaagg cgtggtaatc ctccgttcca
    11041 gttccgttta gatcctgact tacgggaaaa gatggaaaag gcacaacaac aggacggcga
    11101 tgattctctt gctgcctgga taaagcgaat aatccgcaaa gaattacagc aacgcggcat
    11161 agaacccaaa ggataaaaac tatcagcgcc gtggtgtgag gtactacggc gcattgcttt
    11221 acaggcgcac acaatgacca acgaagaatc aaccgaaaaa acatcacctg cacgaaaaag
    11281 acgacgcaga aagaaagagc atgaatcaga acgattcgcg ccgtgttctt ttgcccttga
    11341 gaagtacctt aaagaatact catcaaagga gagaacttcg cagttatggc aacgcactga
    11401 accagactga ttgcattgcc caccagcctg atagcggcta tcatccccgt gcttatgttt
    11461 gggatcacac acacaaacgg cgcagcgggt tatctgttca gaaaggcggc tccatttcgg
    11521 ggccgctttt tttatgcctg aaaaacccca attttgtggt tttccagttt caccagggcg
    11581 aacgaatccc cgcccacgtt ctggcgtata ttcaatcttc atggttatag ctctgtgttc
    11641 agatgattga tgtgtggcgg ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct
    11701 atccttccag gccagaacct cggataaaga ccagccaacg gaacgaccgc caagtttacg
    11761 acgtgatggg aattgtccgg ccttttccag gcggtagcgg catgagcggc taaggcctgt
    11821 tagcttttcg cattcttttt cacgtataaa ccgatcagtg cttaacacta ttgccccctt
    11881 tcgtttctta aagagttatt tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg
    11941 atgattcaga gttggcaaat gttgaggccg tatggtttac agaaagagga ataatcagga
    12001 taaaatcatt taaattcatg ttaatacaaa ggcataaaat attgtttcat gccttttttc
    12061 tcgctattta aagagtgatt cgctagtgta taaaaaacca gtaagacgtt aaaaatcagc
    12121 tacttataaa tctgtacact ttttcacctc ttgttcgtag ttgttccgca ttgttgctca
    12181 ttgttgctca ttgttgctca ttgttgcacg ttgtatctgt tcgcatatcc agtatgcgca
    12241 tactgaaaaa acacgaaaaa aattattttc ttctggctac tggtagcgtg gttacgtttt
    12301 catgtgttcc cgccagtatc cctaaccgct ccgtccacat atccagcgca ttgcgtttag
    12361 catccagata acgggaatga ttataaactc gctgcattcc tggcatctgg tggcctgtaa
    12421 gctgctccac gacgtgagga tccacgccta aatcgttcag catcgttgta aaggtgcgcc
    12481 ggatgtcatg tagtgaccag tgagggtgat ttagcctcct gtgcgctaat ctgccgtact
    12541 gcgacacgct tgtttcctgt ttcacttccc ccagcaataa gcccgtgtgc ctgttctgct
    12601 ccaccagctg cgtgacgaac ggcagtattg cttccggtat gggccggaat attgccacct
    12661 tcgttttgct gtgttctttc ggcacggtcc agagcatttc ggtaaaatcc cactcgctga
    12721 tctccgataa cctcagttct accgtccggc atccgaacac aatcaggagg cggattaacg
    12781 cgatgtagta gggggagaat atttttttgt ccagtgcctg caataattcg cccagttctt
    12841 tggtgcttaa gacacgctcg cttatatccg gttttttccc aacgtccgcc acactcatat
    12901 catcaagaac gttgctgatt gcatagcgcc gcctccggca gaacttaagc gcctgtttgc
    12961 acgtctgtag caagaatccg gcagtaacag gcgttcgctt tgccacctgg tcaaaacagg
    13021 ccagccagtg ccgtagctcg catttatcca gcggcatagc accaatgtgc tgtattacgt
    13081 gattattaag gcgctttttc agggcggcat aatccacgcg gttttccttt gcgtacgact
    13141 caagccagta ggtgagcgca tcgccaaccg ttaccggctt taacgcttcc tgtacggtgt
    13201 aattaagctc atgacgtgga tttttcccct ctgccagcca tgcgcgacac tgggcggctt
    13261 tttccctggc tgatttcagg ctcagatcag gataatttcc cagcttaatg cgttccggtg
    13321 gtgcccccct tcccgttccg gccctgtaag tgaaatacca ggttaaaagg ccactggttg
    13381 aatgcctgac gctcaggttt ccaccgtcat taagaaaggc tgttttttgg gcgggtgtgc
    13441 cgttgatttt cctcagctgt gtatcgctca gtttgttaag tgctctgctc ataattttga
    13501 ttccggtcat cacaattaac tacaccatta actacaccga tcgttgcaca aagggctaca
    13561 acgtgaaaca agctggaaca aggaaaatca caaatctgtt tataatcaaa aacataatga
    13621 acaatctgaa acattatgaa acggcaaaaa acactaattg ataaaatatg tccatacttc
    13681 acgcattacg ttaagcatcc gttataatcg gttgcagata ccagcctgtg gatgcttaac
    13741 atggaatacc aactcactct taactggccc gattttcttg aacgtcactg gcagaaacgc
    13801 ccggtggtgt taaaacgcgg ctttaataat tttattgacc cgatctctcc agacgagttg
    13861 gcgggtctgg cgatggaaag cgaagttgac agtcgactgg tcagtcacca ggatggcaaa
    13921 tggcaggtca gccacggccc gttcgaaagc tacgatcatc tcggtgaaac caactggtca
    13981 ttactggtac aggcagtaaa ccactggcat gagccgaccg ccgcgctgat gcgaccgttc
    14041 cgtgaactac cggactggcg tattgatgat ctgatgattt ctttttctgt acctggcggc
    14101 ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca ttcagggtac cggacgtcgt
    14161 cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact gcccacatcc agatctgtta
    14221 caggtcgatc cgttcgaagc catcatcgat gaagagctgg agcctggtga tattctttat
    14281 attccgccag gattcccgca tgaaggctac gcgctggaaa atgcgatgaa ctattccgtg
    14341 ggttttcgcg cgccaaatac gcgggaatta attagcggat ttgccgatta tgtgctgcaa
    14401 cgtgaactgg gcggcaacta ctacagcgat ccggatgttc cacctcgcgc tcatcctgcg
    14461 gatgttctgc cgcaagagat ggataaactg cgtgagatga tgctcgaatt gatcaaccag
    14521 ccggaacact ttaagcaatg gtttggcgag tttatatccc agtcacgtca tgaactggat
    14581 atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct acgatgcact gaaacaaggt
    14641 gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca ttggcgacga cgtgtatgcc
    14701 aatggtgaga agatcgattc cccgcaccgt ccggcactgg atgcactcgc cagcaacatt
    14761 gcgctgactg cggagaattt tggcgatgcg ctggaagatc cgtcattcct cgcgatgctc
    14821 gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt aagtttggat ttaagccgga
    14881 tgcagcatca catgccgcat cccttttcgc ttacttccgt tgcgccgtta actcggcaat
    14941 acggacgatc acctgcaccg ctttttccat accttccaga atcacaaact catgcttacc
    15001 atgatagttg taaccgccag tgaacaggtt cgggcaaggt aatcccataa acgacaactg
    15061 cgcgccgttg gtaccgccgc ggatcggttt cagttccggt tcaatatcgc agtcacgcat
    15121 cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc actttctcgc gcatattgta
    15181 gtaactgtct tcaatcacca attcaatgta gcaatcagga tgtaaccctt tgcccacttt
    15241 tttggcgatc tccatcattt tacgtttacg cgcttcaaac tgtttacggt cgaaatcacg
    15301 gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc atgctcgcca ggtgatagaa
    15361 accttcatag ccttctgtca tttccgggct ttcatccgcc ggaacttccg catgaatacg
    15421 tgccgccagc gacagcgcat ttaccatcac tcctttcgcc gtgcccggat gaacattgtt
    15481 accgacaatt ttgatattga ccgatgcggc gttgaagttt tcaaactcca gttcgcctac
    15541 gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag gcatcaacat caaaatgttt
    15601 cgcccctttg cccacttctt catccggggt aaaggcgacg cgaatatcac catgcggaat
    15661 gtttttctgt tgcaataccg ccagcgcggt catgatttct gcaatacctg ctttgtcatc
    15721 ggcacctaac aaggttttac catcggtggt aatcaacgtc tgacccagta gctgatgcag
    15781 caccgggaac ataaccggtg ataaaacttc atcgccgata cccagcgcaa tatcgccacc
    15841 gcgatagttt tcaacaattt gcggattaac atttttgccg ctgcaatccg gtgaggtatc
    15901 cacatgagaa ataaagccaa tcgccgggat atcgccaggg acgttagccg gtaacgtcgc
    15961 catcaaagtg cccttctcac ttaaggtcac attgataagc cccatttctt cgagctgctc
    16021 tttcagcaga tgcaataact tccattggcc ttccgtgctg ggaacctgtc tcacccctgc
    16081 ttttgattgg gtatccagag acacgtagtt caaaaatcgc tcaagtagtt tatccatgta
    16141 gtcaccctca ctttttgtga caacattatt aagaagcaag aaaagacaaa tattgcgtca
    16201 ggtcactttt acccctgcaa gcgggaatat ttatcagcat taccttaatg aatataaagc
    16261 taagccagta attcacaaca aagattggcg attcaagcgg tttgccgtag aattaccgcc
    16321 ctcattaaga gtcaccaagg tggttaacca caaaccccgc atcggtaagc catccgttgc
    16381 gtttacatgg gacagagtaa aaaattgaat aaacaaccga gttcgctttc accgctggtg
    16441 caattggcgg gaattcgcaa atgctttgat ggtaaagagg tcattcccca gctggatctg
    16501 actatcaaca atggcgagtt cctcacgctg cttggccctt ctggctgcgg taaaacaacc
    16561 gttcttcgcc tgattgcagg tctggaaact gttgattccg gacgcatcat gctggataac
    16621 gaggacatca cccacgttcc ggcggaaaac cgctatgtga acactgtttt ccaaagctat
    16681 gcacttttcc cccacatgac cgtgttcgaa aatgtggcct ttgggttgcg catgcaaaaa
    16741 acccccgctg ctgaaattac gccccgcgtg atggaagccc tgcggatggt gcagctggaa
    16801 accttcgctc aacgtaaacc gcatcagctc tctggtggtc aacagcaacg cgtcgccatt
    16861 gctcgcgcgg tggttaacaa gcctcgtctg ttgttgctgg atgagtcgct ctcagcgctg
    16921 gattacaaac tgcgtaagca aatgcagaac gagctgaaag cgttacagcg taagcttggc
    16981 attacattcg ttttcgtgac tcacgaccag gaagaagcac tcaccatgtc ggacaggatt
    17041 gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc cgcgtgaaat ctacgaagag
    17101 ccgaaaaacc tgtttgttgc cgggttcatt ggcgaaatta atatgtttaa cgccacggtg
    17161 atcgagcgac tcgacgagca gcgcgtacgc gccaacgttg aaggccgcga atgtaatatc
    17221 tacgttaact tcgccgttga accggggcaa aaactgcatg ttctgctgcg cccggaagac
    17281 ttacgtgttg aagagattaa cgacgacaac cacgctgaag ggctgattgg ttacgttcgt
    17341 gagcgtaact acaaaggcat gacgctggag tcggttgttg aactggaaaa tggcaagatg
    17401 gtgatggtca gcgaattctt caatgaagac gatcctgact ttgaccactc tctcgaccaa
    17461 aaaatggcca ttaattgggt agaaagctgg gaggtcgtac tggctgatga agaacacaag
    17521 taagttccag aatgtagtga ttgtcactat tgtcggttgg cttgtgttgt ttgtctttct
    17581 gcccaacctg atgatcattg gcaccagctt tttgacccgc gacgacgcca gtttcgtcaa
    17641 aatggtcttt acgctggata actacacgcg tctgctcgat ccgctctatt ttgaagtgct
    17701 attgcactcg ctgaatatgg cgctgatcgc caccctcgcc tgcctggtgc tgggctatcc
    17761 atttgcctgg tttctggcga agctgccaca caaggtgcgt ccgctgctgc tgtttctgct
    17821 gattgttccg ttctggacca actcattgat tcgtatctac gggctgaaaa ttttcctcag
    17881 caccaaaggc tatctcaacg agtttttgct ctggctgggc gttatcgaca caccaatccg
    17941 tatcatgttc acgcccagtg cggtgattat cggtctggtt tacattctgc tgccgtttat
    18001 ggtgatgccg ctgtactcca gtatcgaaaa actggataag ccgttgcttg aggcggcgcg
    18061 cgatctcggt gccagcaagt tacagacttt tatccgcatc attattccac tgacgatgcc
    18121 ggggattatt gccggatgtc tgctggtgat gctgccagcg atgggcctgt tctatgtgtc
    18181 cgacctgatg ggcggtgcga aaaacctgct gatcggtaac gtcatcaagg tacagttcct
    18241 caatattcgt gactggccgt ttggtgcagc caccagcatt acgctgacta tcgtaatggg
    18301 cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg aataagaagg tggaactcga
    18361 atgatcggtc gactgcttcg cggcggtttt atgaccgcta tctacgcgta cctgtatatc
    18421 ccaatcatta ttttgattgt gaactccttt aacagctcgc gctttggcat caactggcag
    18481 ggttttacca ccaaatggta tagcctgctg atgaacaacg atagcctgtt acaggcagcg
    18541 cagcattcac taacaatggc ggtgttttcg gcgacgtttg ctacgcttat cggttcactg
    18601 acggcagttg cgctgtaccg ttatcgcttt cgcggtaagc cgttcgttag cggaatgctg
    18661 tttgtggtaa tgatgtcacc agatatcgtg atggcaattt cgctgctggt gctgtttatg
    18721 ctgctgggta ttcagcttgg cttctggtcg ctgctgttct cgcatatcac cttctgcctg
    18781 ccatttgtgg tggtgacggt gtattcgcgc ctgaaaggtt ttgacgtgcg gatgctggaa
    18841 gcggcgaaag atctcggtgc cagcgaattt accattctgc gaaaaatcat tctgccactg
    18901 gcaatgccag cggtggcggc gggctgggtg ttaagcttta ccctgtcgat ggacgatgtg
    18961 gtagtttctt cattcgtcac cggaccaagt tatgaaattc tgccgttaaa aatttattcg
    19021 atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg caaccatatt actggtgctg
    19081 tcgctggtga tggttattgc cagccagctt attgctcgtg ataaaacgaa aggtaacaca
    19141 ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg
    19201 gcatgagcgc cgctcacgcc gatgacaaca acacgctgta tttctacaac tggaccgagt
    19261 acgtgccgcc aggactgctt gaacagttca ccaaagaaac cggtattaag gttatctatt
    19321 cgacttacga gtcgaacgaa accatgtacg ccaagctgaa aacgtacaaa gacggtgcct
    19381 atgacctggt ggttccttca acctattacg tcgataaaat gcgtaaagag gggatgatcc
    19441 agaagatcga caagtcgaag ttaacgaact tcagcaatct cgatccagac atgctcaaca
    19501 agccttttga cccgaataac gactactcca ttccgtatat ctggggtgcg acggcgattg
    19561 gtgttaacgg tgatgcagtg gatccgaaat ctgtcaccag ctgggccgat ctgtggaagc
    19621 cagagtacaa aggcagcctg ctgttgaccg acgatgcccg tgaagtgttc cagatggcgc
    19681 tgcgtaagct gggctactcc ggtaacacca ccgatccgaa agagattgaa gctgcatata
    19741 acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa ctccgataac ccggctaacc
    19801 cgtacatgga aggcgaagtt aacctcggca tgatctggaa cggttctgct ttcgttgcac
    19861 gccaggcggg tacgcctatt gacgtggtgt ggccgaaaga aggcggcata ttctggatgg
    19921 acagcctggc gatcccggca aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact
    19981 tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa
    20041 accttgcggc gcgtaagctg ttaagtccag aagtggcaaa cgataagacg ctctacccgg
    20101 atgctgaaac cattaaaaat ggcgaatggc agaatgacgt tggcgcagcc agcagcattt
    20161 atgaagagta ttatcagaag ctgaaagcag gacgttaatt caacaagcgg acggtggtat
    20221 taccgcccgt tttttagtta gcaaggatgt ggctaatgag tcaggacagt aaagttttat
    20281 cccgctcttt cgtcggtatt gtggtccgct tgtaggcctg ataagcgtag cgcatcaggc
    20341 aatgcttcct gctttataat ccctccagca acttttcaac aaattctggc accacctggc
    20401 ttgccgggcc gtaatatttc tcggcaaatt cattaccaac ctgactcggt tcaagattca
    20461 gttcaacggt gtgagcacca tgtagtttgg cttcgtgtac aaacccagca gccggataga
    20521 catgcccgga agtaccaatg gcaataaaga catccgccat cgacaacgcc atataaattt
    20581 catccatgcc gagtggcatt tcgccaaacc aaacgacgtg cggacgcaag ggtgccggga
    20641 agtggcaaca atggcattta tcttctggcg taacgtctcc ggtccagtcg agaacctgac
    20701 cactttgcga acaacgaact ttcagcaatt ccccatgcat atgaatgaca ttggtattac
    20761 ctgcgcgttc atgcaggttg tctatattct gcgttaccag caaaaagcga tcgccgagag
    20821 cgtcttgcag tttagccagc gcaagatgcg cggcgttagg ctgaatttct ggctgctgca
    20881 gctgtcgacg acgggcgtta taaaacgctt gcaccagttc aggatcgcga tcgaaacctt
    20941 ccggcgttgc cacatcttca acccgatgtt cttcccacag gccatctgcg gcgcgaaagg
    21001 tacgaatacc tgattccgca gaaattcctg cccctgtcag tacgagtact cttggttttt
    21061 ccattgcttc cggcaccact ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca
    21121 ggcgacgttt atttttacga aaacgactta accgatgacc ccgacgcgac agcatagcaa
    21181 cctctgttgt ttaatcggtt agatgtagga aggccgcacc acgcattcct cccgcatcac
    21241 cgtggcgcgc gcgttcaatg cgcggaacac gagctacagg taagagatga caaggcaacc
    21301 tgtccgccag ttgcgttgtg attgccggga aattcgataa gccaccacca atgacgacca
    21361 ggtcagggtc aacaatggtc aggatatttc ccagagaaac cgctaataaa tccagataac
    21421 gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata aagcgcaata atttcgggag
    21481 cctgcaacgg ttgatgataa tagtgttgat acagccacgc aaaaccgcga ccagacagat
    21541 aattttcaat gcagccatgc tgaccacagc cgcagcggcg taacgggaaa tccaacccca
    21601 tcatggttaa cgcatcaacc ggcagacgca tatggccaaa ctcgccggta atgtagcttt
    21661 taccagtaat cggtttgccg ttgaaaacca gcccgccgcc aacgccggtg ccgagaatta
    21721 accccatcac cagtggatat tgcgtaaatt catcctccca ggcttctgaa agggcaaaac
    21781 agttggcatc gttatcaagg cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca
    21841 gcggtttgcc gctggcagcg gggacattag cggcatacag cttaccatct tctgtttccg
    21901 gcattcccgg aataccgatg ccgacagaac ctttacagcc aaaacgttgg tcagcttcag
    21961 ctaccagctc acacactgca tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc
    22021 gcttttccca ctgcaactgc cgaccgctat caaacacgcc aagcgcaatt tttgttccac
    22081 caatatcaaa cccgtaatac attgccgctc cttgttttaa tgtactgcct ttactggccg
    22141 ctaaggactc gcgcagggtc aatattactg gcgcgccgcg ccggatacca acttgccaaa
    22201 agactcagca acaatgctgt gaccagtacg tagaagacgt ccagccagtg caattccgat
    22261 ggcaggaagt caataaaata gatatcgctg gagaggaact gatgaccgat cagcttttca
    22321 atccactcaa taatcggggt aagttgcagt gaaacaacta cgccgataac caccccacac
    22381 aggctgccga acagccctgc cagcaatcca taccagacaa agatggcgcg aattaaacca
    22441 tctttcgccc ccagcgttct taatactgcg atatcgccac tcttgtcttt caccgccatc
    22501 actaaggtgg agacgatgtt gaaacaggcc acgccaatca ccagtaccat cgccagatac
    22561 ataatggcgc ggatcatctg gatatcgcga tacatatagc cgtaagtacc aatccaactt
    22621 ttaatataaa catagctgtt ggtcacttca cccgcatcgc gtaccagctt attggcgttg
    22681 aaaacatccg tcattttaag ggcaatacct gacacgctgg aacccatatc aagatattgt
    22741 tgggcatccg ccagcgggat catggcaaaa ctgtgatcga gttgaccact caactgcaaa
    22801 ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca gtttatgctc gggattcgag
    22861 ttggggatca tgatcgacac ccaatcgccc tgcttcactt tcagcgcatc cgccacgcct
    22921 ttgccgatga taatttgctg ttcgcccgct ttaaaattgc gccacgcatc cccctgaaca
    22981 aacgagggta atgcgctcag acgctgttcc tgttgtgggt taacgccctt cacctggatt
    23041 gcgcgcagat tcgctccact ttccaccaga ccggtgaaat tgatatacgg cgcagcggcg
    23101 gcaatacccg gcactttctg cacgttatcc agtgcttcct gccagttagt ccatggctga
    23161 tttaccgctt cgatttcgcc atgcggcacc accgccagaa tgcggttatt cagttcgcgt
    23221 tcaaagccgt tcatcgcgct taagccgacg atcaataccg ccacgccgag ggcaatgcca
    23281 atggtagaaa tgacagagat tagcgacacc atgccgccgc gtcgccgtcc gcgactaaaa
    23341 cgcaggccaa tcaataacga taaaggcatc gccattactc cgcccccatc aggctcagtt
    23401 ccgccgtcag acgtccatca cgcatttcca gttggcggct catacgtttc gccagttgca
    23461 ggtcgtgagt gaccaccagg aaagcggtgc cctgcaagcg attcaactct ccaagcaact
    23521 ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt aggttcatcc gccagtacca
    23581 gacgcggatt attgaccagc gcacgggcaa tcgctacacg ctggcgttcg ccgccagaaa
    23641 gttcagatgg gcggtgattc gcacgatgct ccagccccac cgcttttaac atctccagtg
    23701 cacggctgtt gatttcagcg ggctttttct tgccaatcag cagtggcata gccacgtttt
    23761 ccagggcagt aaaatccggc agcaggtggt gaaactgata aataaagccc agcttctggt
    23821 tgcgcagttc tgctttcgcc gctgaagaca gtttgctcat cggctgaccg ttaaagatca
    23881 catcgccgga ggttggcgta tccagcccgc ctaacaggtg cagcaaggta cttttaccgg
    23941 aaccagagct accgacaatt gccatcattt caccttcgcc cacgctgaaa ctgacattgt
    24001 gcagcacatc ggtttgcaca ctgccttcct gatagcgttt gcacaggttg tcgcattgca
    24061 acaggatctt attcataacg taaagcctca gcgggttgtg tggcggcagc gcgccatgaa
    24121 gggtaaagcg tagacagcag cgcgatagcc atcgccacca gcgcaataac aatgacctgt
    24181 aaaggttcga tagccaccgg cagcgccgcg ccatcaagca ggacgccgat tatcggcatc
    24241 agattattta actggctggc aagcagtgcg ccaagcgccg ctccgaggat tgcaccgata
    24301 atcccggcgc tggccccttg caccataaag accatcatga tttgtcgcgg agttaagcct
    24361 tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca ccatcagccc cagcgaggta
    24421 ataatgttaa acgccgcaac agcgacaatc aggctcagca gtaagcccat catatttttt
    24481 tccatgcgta cggcctggaa cagctcgcct ttacggtcac gccagtcctg ccatttgctg
    24541 ccttcaggca gtttttgctg acttaaagag tcaactttca gcggctcatc cagccacaaa
    24601 cgccagccgg taatattggc tgccggataa cgcatcagac gcgaggcatc ctcaatattc
    24661 accagcattt catagccatc gacttcactg ttggcggcga aagtgccaat cacattgaac
    24721 aggcgctgac ttggaatacg ccccatcggc gtgaactggc tggcagatgg caccatcacg
    24781 cggatttgat caccgcgatt gacgcccaac tgtgaggcaa gttgttcgcc gaggatgaca
    24841 ttatatttcc ccggctcgag gtcagtttgt ttcacattga ccagatacgg cgttagtgga
    24901 tctttttgcg ccgggtcgat accgagcatc accccgaccg ccacgctgcg cgcgctttgc
    24961 agtaccacat caccggtagt aataggtgcg acgcgattaa cgccgtccag tttgactgcc
    25021 gtttccggga gttgctgcgg gttaagagag ccatgctcag aagagagaat tgcctgtggc
    25081 atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc cgttcatcac tgacaatact
    25141 gtgaccagcg ccatcacccc gagggtaatg ccgatggtag aaagccagga gacgaaacga
    25201 ccgaagcgat ccgctgcacg cccacgcatg taacgcaagc caataaatag agcgacaggt
    25261 tggtacatga aatccgtctg gttgctgtag caaagacacg agtatataag cgaaagcaag
    25321 atgggtaaac gtctgaactg taagtgatcg tcagggctaa tctgaaaagc ccccgtaaat
    25381 caatcggcta aattgccatg ttttatcatc aatcttgccg cctgatctta atctgaaaaa
    25441 tcatccaata cagacagata cccattacac ctcgggtcat aaccagcagg atcaaccgca
    25501 tgtaaattaa aaaagagcgt gttgttctga tgaaacaaaa agagctatgg attaaccaga
    25561 tcaaagggtt atgtatttgt ctggtggtga tttatcactc ggtcattacc ttttatccgc
    25621 atatgaccac ttttcagcat ccgttatcgg aagtcctgag caaatgctgg atctatttca
    25681 atctttacct tgcccccttt cgtatgccgg tttttttctt tatatccggc tatttgattc
    25741 gccgctatat cgacagcgta ccgtggggaa attgtctcga taaacggatc tggaacatct
    25801 tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc acctgagcgc gatttaagca
    25861 atgcctccaa tgccgcttat gccgattcca ccggtgagtt cctgcacggg atgatcaccg
    25921 ccagcaccag cttgtggtat ctgtatgcgt taattgtcta tttcgtggta tgtaaaattt
    25981 ttagccgtct ggcgctgcca ctattcgcct tgtttgtact gctgagtgtg gcggttaatt
    26041 tcgttcccac gccgtggtgg ggaatgaaca gtgtgatacg caatttgcct tattacagcc
    26101 ttggcgcatg gtttggcgca acaataatga cctgtgttaa agaggtgccg ttgcgccgcc
    26161 atctgctgat ggcttctttg cttaccgttc tggcggtcgg tgcctggttg tttactatct
    26221 cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa gctgttttat cagtacgagc
    26281 aacgattcgg tatgcgctcc accagcctgc tgaatgtgat tggctccaac accattgcta
    26341 tctacaccac ccatcgcatt ctggttgaaa tattcagctt aactctgctt gcgcaaatga
    26401 acgcagcacg ctggtcgccg caagtcgaac tgacactcct gctggtttat ccctttgtca
    26461 gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa actttcacag cgtgcattca
    26521 gcgatctgtt gttctccccg ccttcactgc ccgctgccgt cagttactct cgttaaacca
    26581 tcaccccagc cggggcgatt caatttgcta aaccatgtcg attacggata atgaacagca
    26641 ttgcttatca gagatatgcc cccatatgtt gaggcatatc ctaacgagaa tctgacaacc
    26701 gttatgcctg aacaatatcg ttatacgctg cccgtcaaag cgggtgagca gcgtctgctg
    26761 ggcgagttaa ccggcgcagc ctgtgcaacg ctggtagcgg aaattgccga acgtcacgcc
    26821 ggtccggtgg tactcattgc accagatatg caaaatgctc tgcgtttgca tgatgaaatc
    26881 agccagttca ccgatcagat ggtgatgaat ctggcggact gggaaactct tccctacgac
    26941 agtttttcgc ctcatcagga cattatctcc tcgcgccttt ccacccttta ccagctaccg
    27001 acgatgcagc gtggcgtact gattgttccg gtgaatacgc ttatgcagcg cgtttgccca
    27061 cacagttttc tccacggtca tgcgctggtg atgaaaaaag gtcagcgcct gtcacgagat
    27121 gcattacgaa cccaactgga cagcgccggt tatcgctatg ttgaccaggt gatggagcac
    27181 ggcgaatacg ccacgcgcgg cgcgttgctg gatctcttcc cgatggggag tgagctgcct
    27241 tatcgtcttg atttctttga tgatgaaatc gacagcctgc gggtgtttga cgtcgacagc
    27301 cagcgcacgc tggaggaagt agaagcgatc aatctgctgc ccgcacacga atttccgacc
    27361 gataaagcgg caattgaact gttccgcagc cagtggcgcg ataccttcga agtgaagcgc
    27421 gatccggaac atatttacca gcaagtgagt aaaggcacat tacctgccgg gatcgagtac
    27481 tggcagccat tgttcttcag cgaaccactg ccgccgctgt tcagttattt ccctgccaat
    27541 accttgctcg tgaatactgg cgatctggaa accagtgccg aacgtttcca ggctgacacg
    27601 ctggcgcgtt ttgagaatcg cggcgtcgat ccgatgcgcc cgctgttgcc accacaatcg
    27661 ctctggctgc gggtggacga gctcttctca gagctgaaaa actggccccg agtgcagcta
    27721 aaaactgaac atttaccgac aaaagccgcg aatgccaatt taggtttcca gaaactgcca
    27781 gacctggccg ttcaggcgca acaaaaagcg ccgctggatg cgctgcgtaa gttcctcgag
    27841 actttcgacg gtccggtggt gttctcggta gaaagtgaag gtcgccgtga agcgctgggt
    27901 gaactgctcg cacgaattaa aattgctccg caacgcatta tgcgtcttga tgaagccagc
    27961 gaccgtgggc gttatctgat gattggcgct gccgaacatg gttttgtcga taaggtgcgt
    28021 aatctggcgc tgatctgcga aagcgatctg ctcggtgaac gtgttgcccg ccgtcgtcag
    28081 gattctcgcc gcaccatcaa ccccgataca ctgatccgta accttgcgga gctgcatatt
    28141 ggtcagccgg tggtccatct ggagcacggc gtcggtcgct atgccggaat gaccacgctg
    28201 gaagctggcg gcattactgg cgagtacctg atgctcacct atgccaacga cgccaaactg
    28261 tatgttccgg tgtcgtcact gcatctgatt agccgttacg caggtggcgc ggaagaaaac
    28321 gccccgctgc ataaacttgg cggcgatgcg tggtcacgcg cgcggcagaa agcggcggaa
    28381 aaagtgcgtg atgtggcggc ggaattgctg gatatctacg cgcaacgcgc cgccaaagag
    28441 ggcttcgcgt ttaaacacga tcgtgagcag tatcagttgt tctgcgacag cttcccgttt
    28501 gaaaccacgc cggatcaggc gcaggccatt aatgcggtac ttaacgacat gtgtcagccg
    28561 ctggcaatgg atcgtctggt gtgcggcgat gttggctttg gtaaaacaga agtggcgatg
    28621 cgcgccgctt tcctggcagt agataaccac aagcaggtag cggtgttggt gcctaccacc
    28681 cttcttgcgc agcagcatta cgacaacttc cgcgaccgtt tcgccaactg gccggtacgt
    28741 atcgaaatga tctcccgttt ccgcagcgcc aaagagcaga cgcaaatcct tgcggaagtg
    28801 gcggaaggga aaatcgatat tctgatcggt acgcacaaac tgctgcaaag tgacgtcaag
    28861 tttaaagatt taggcctgct gattgtcgat gaagaacacc gcttcggggt gcgtcataaa
    28921 gagcgcatta aagcgatgcg cgcgaacgtg gatattctga cgcttactgc aacgccgatc
    28981 ccacgtacgc tgaatatggc aatgagcgga atgcgtgacc tgtcgattat cgccacgccg
    29041 cccgcccgtc gtctggcagt taaaaccttt gtccgtgagt atgacagcct ggtggtccgg
    29101 gaggcgatcc tgcgtgaaat tttgcgcgga ggacaggttt attatctcta caatgatgtg
    29161 gaaaacattc agaaagccgc cgaacggctg gcagaactgg tgcctgaagc acggattgcc
    29221 atcggtcacg ggcagatgcg cgagcgcgaa ctggaacggg tgatgaatga tttccatcat
    29281 caacgtttca acgtgctggt ttgtaccacc attatcgaaa ccgggatcga catcccgaca
    29341 gccaacacca ttatcattga acgtgcggat cacttcggtc tggcgcagct gcaccagtta
    29401 cgcggtcgcg tcggacgttc acatcatcag gcatatgcat ggctgctgac gccgcatcca
    29461 aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga ttgcctcgct ggaagatctc
    29521 ggggcaggtt ttgcgctggc aacgcacgat ctggagattc gcggcgcggg tgaactgctt
    29581 ggcgaagaac aaagcggctc aatggaaacc atcggtttct cgctgtatat ggagttgctg
    29641 gaaaacgccg tcgatgcact gaaagccgga cgcgagccgt cgctggaaga tctcaccagc
    29701 cagcaaacag aagtcgagct gcggatgccg tcgctattgc cagatgattt catccctgac
    29761 gtgaacacgc gtctgtcgtt ctacaaacgt attgccagcg ccaaaacgga aaacgaactg
    29821 gaagagatca aagtcgagct tatcgatcgc ttcggcctgc tgccagatcc ggcgcgtacc
    29881 ctgctggata ttgcccgtct gcgccagcaa gcgcagaaac tggggatcag gaagctggaa
    29941 ggtaatgaga aaggcgggat gatcgaattt gccgagaaga atcacgttaa tccggcctgg
    30001 ttgattggtt tgctgcaaaa acagccgcag cattaccgcc ttgatggtcc gacgcgcctg
    30061 aaatttattc aggatttgag tgagcggaaa acgcgtatcg aatgggtacg ccagtttatg
    30121 cgtgaactgg aagagaacgc gatcgcttaa tgtaaatcgg gggcgtttct gcgcccccat
    30181 ctgcaacatt tacaaattct ttgcactttc ctgcactatc cgacactgtc accatccata
    30241 attcaggctt atctgtttat tacaataacc ttatatttat tatggatttt tggtgatgat
    30301 caaaacgcgt ttttctcgct ggctaacgtt ttttacgttc gccgctgccg tggcgctggc
    30361 gctaccggca aaagccaaca cctggccgct gccgccagcg agcagtcgtc tggttggcga
    30421 aaacaaattt catgtggtgg aaaatgacgg tggttctctg gaagccatcg ccaaaaaata
    30481 taacgtcggc tttctcgctc tgttacaggc taaccccggc gttgatcctt acgtaccgcg
    30541 cgcgggcagc gtgttaacga tcccgttgcc aaccctactt ccagacgcgc cgcgcgaagg
    30601 cattgtgatc aacattgcgg agctgcgtct ctattactac ccgccgggta aaaattcggt
    30661 aaccgtgtat ccaataggta ttggtcagtt aggtggtgac acgctgacac cgacaatggt
    30721 gaccaccgtt tcagacaaac gtgcaaaccc aacctggacg ccaacggcaa acatccgcgc
    30781 ccgttataaa gcacagggaa ttgagttgcc tgcggtagtg ccggctggac cggataaccc
    30841 aatgggccat catgcgattc gtctggcggc ctatggcggc gtttatttgc ttcacggtac
    30901 gaacgccgat ttcggcattg gcatgcgggt aagttctggc tgtattcgtc tgcgggatga
    30961 cgatatcaaa acactcttta gctaggtcac cccaggcacc aaagtgaata tcatcaacac
    31021 tccgataaaa gtctctgccg aaccaaacgg tgcgcgtctg gttgaagtac atcagccgct
    31081 gtcagagaag attgatgacg atccgcagct gctgccaatt acgctgaata gcgcaatgca
    31141 atcatttaaa gatgcagcac aaactgacgc tgaagtgatg caacatgtga tggatgtccg
    31201 ttccgggatg ccggtggatg tccgccgtca tcaagtgagc ccacaaactc tgtaaatcct
    31261 ggataaaaaa aatccccgcg acacggcatg tcgcggggaa aaaatcagca gtagcaggca
    31321 ttaatgaggg ttaatgctta tttataaatt actgctgttc catggagggt attcggaccg
    31381 gttacagaag taatacggaa agattttgcg cccatctcat ccgctttttg cgccagctgc
    31441 tcttccagcg atcccagatt tgtccccgcg ttagcactga tagtaccgac tttttgttgg
    31501 ccttctggcg ttgactgaac ttcgacagcc gcaaagttgg caaatgacat ggagcttaaa
    31561 atcgccgcag cgatgagggt ttttacgttt ttcataatag tggccttatg cagatgaatg
    31621 acgtcgcaag aaattactta acgatcgata actaaatgat agatgtgatc tggatcacat
    31681 acaagatatt ttttataaca atcattaatt aatttaaaaa tctttaaatt tcagtcaaat
    31741 agaaagcaat tatttttatg ggatctggac tggtgaacgg aggtgattat ttttataata
    31801 acctttcatt cacagtcacc aggtacaacg gcatcatggc aactgactca acacaatgtg
    31861 taaaaaaaag ccgtggccgc ccaaaagtgt tcgacaggga tgccgcgctt gataaggcca
    31921 tgaaattgtt ctggcaacac ggttatgaag cgacttctct tgcggacctc gtcgaagcga
    31981 ccggagccaa agcgcccacg ctatacgcgg aatttaccaa caaagagggg ttatttcgtg
    32041 ccgttctcga ccgctatatc gatcgttttg ccgctaagca tgaagcacag ctgttttgtg
    32101 aagagaaaag cgtggagtct gcgctggctg actattttgc tgccatcgcc aactgcttta
    32161 ccagcaaaga caccccggca ggctgcttca tgatcaacaa ctgcaccacc ctctccccag
    32221 attcaggaga tatcgccaat acgttgaaat cacgccatgc gatgcaagag cgcactttgc
    32281 agcagttttt atgtcaacga caagcgcgcg gggaaatccc gacccactgt gacgtgacac
    32341 atctggcaga attccttaat tgtattattc aggggatgtc gatcagcgca cgcgaaggtg
    32401 catcgctgga aaaactgatg cagattgccg gaacgacttt gcgtttatgg cccgaactgg
    32461 tgaaataagt cgcaaaaaaa gcccctcagc ctgagcgaat gaggggccaa attgcagagt
    32521 acagccttgt tatactttgt tattcaaaat cagttgcccg ttactatcta gcgggatctg
    32581 cgtacccgga tcgcggtcca tgcggatttt gccctgctga tcgccaatct tataggtcac
    32641 atcataaccg agcatttttt ctgacttgtc atacaccgtt ttacaacgct gttgcgtagt
    32701 cgtgtaagta tcgctttcct ggagagagcc ctggatctgg ttaccggcat atccaccacc
    32761 cagcgccccc acaacagtgg cgacatcttt accgcgacca ccaccaaact gatgccctat
    32821 cacgccacca gcaacagcgc cgagcaccga cccggtaatg cgattttcat cctgcaccgg
    32881 tcgacgatgg gtcactgtga cgttgcgaca ctcctgacgc ggtgttttaa ccgtttcctt
    32941 gattggggtt gcagaaacaa cctgagcgta ttgcgggccc cgttcaaaca cgttcagact
    33001 ggccactgcc gctacgccca acgcagctgc gacaccaatc ccgatacccg ccaacattga
    33061 tttattcacg ggaacacctc cttctttgca gttatgccaa tcatgcaagc gaaggagaaa
    33121 agcagctatc agacagaaga tgaaaagcgc gggaatgact ggaaatactt aaggattcgg
    33181 aggagtctga aggaatgcct gatgcgacgc ttaacgcgtc ttatcaggcc taccgctcgt
    33241 accatttgtc cagggcggat aaggcgttta tgccacatca gccagtgtac gtcgattaat
    33301 gcaacttcaa acgcggacgg ataacgcggt taatactccc caccagcatc attaatccgg
    33361 ttttaaagta accatgcagc gcaatctgat gcattcggta tagcgagata tatacaaagc
    33421 gcgcaattcg tccttcaatc atcattgagc cgcgcgtcag gttacccatc aggctaccaa
    33481 cggtggagaa gttcgacagc gataccagcg aaccatgatc tttatactga taatttttca
    33541 gcggcttacc gttcatctgc gccagaatgt tgttcattgc gcaagtcgcc atctggtgtg
    33601 cagcctgagc acgcggcgga acaaagcccc cttccggacg cgggcatgac gcgcagtcgc
    33661 caatagcgta aatgtctgga tcgcgagtgg tttgcagcgt cggttccacc accagctggt
    33721 tgatacggtt agtttcaaga ccaccgatat ctttcaggaa gtctggcgct ttgatcccgg
    33781 ctgcccatac catcagatca gcctcaatat attcgccatc tttagtgtgc aggccgcctt
    33841 catcagcact ggtgaccatg gtttgcgtca gcacgcgaac gccaagtttc gttagctcgt
    33901 tgtgggccgc agcagagata cgtggcggta atgcaggcaa aatacgttct cccgcttcta
    33961 ccagcgttac gttcagggct tcgttggtca ggcctttgta accgtagctg tgcagttgct
    34021 tgatcgcgtt gtgcaattca gcggagagtt ctacacccgt cgcgccgccg ccgacaatcg
    34081 caatgttcac tttgccattt gcgcccaggt tggcagagta tttcaggaac agattcagca
    34141 tctcctggtg gaagcgacgc gcctggtgcg ggttatcgag gaaaatgcag ttctctttga
    34201 cgcctggcgt attgaaatcg ttagaggtgc tacccagcgc catgaccagg gtgtcatagg
    34261 cgattttacg ttccggaacc aacagttcac ctttctcgtc gcgtagttct gcaatagtga
    34321 ttgttttcgc ttcacgatca atatcaatga cggaacccaa ctggaactgg aaaccatgat
    34381 tgcgcgcatg ggccagatag ctcaacgcat cgacgccttc atcaagcgag ccagtcgcca
    34441 cttcgtgcag cagtggtttc cacaggtggc tgtggttacg atcgaccagc gtaattttgg
    34501 cttttttctt acgtcccagc ttatgcccca gctgtgttgc catttccagc ccaccagcac
    34561 cgccgccgac aatcacaatc tttttcaatg gcgtagtcaa cgtgaccccc ttaaatttat
    34621 taaccaattg ttaattaatg gttattaaca tagctcttaa ttaacaacag gttacagcaa
    34681 tgaaaatgtt cttcgctatt gagaataaca tgaatggtgc attggtcata ccaaaattga
    34741 tgttaatcaa gttttgttgc ttaaaagata ggcaacttta ccgggggaga tcaaaaaata
    34801 gcgtttatta tttgagcaaa ttcggaagag taatataggg tgaagcgtat atcgagggcg
    34861 tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg actgtcggat
    34921 gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac cggcaaggtt
    34981 acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg ctgtaaatgc
    35041 ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc gtaataatga
    35101 tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga caaaataacc
    35161 agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc aatatcggca
    35221 tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa attagggttg
    35281 aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta tccgcccagg
    35341 ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt gtccacttct
    35401 ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat caagcgtaca
    35461 tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact gttagagtca
    35521 aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt gacctgcttt
    35581 ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgatcgctt tccagcgagc
    35641 cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg taacacgttc
    35701 tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat tattgcagac
    35761 caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat aactgcccat
    35821 aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa aacccagttc
    35881 ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg gatcaacatc
    35941 actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga ttaaggaact
    36001 gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc
    36061 tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat
    36121 tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt tttgtggatc
    36181 ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca gcactggcgc
    36241 aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac ccgcctcttg
    36301 cgccagtttg ccgccctctt ccattcccaa cagcgcagca aaggattgtg ctgccggtaa
    36361 gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct tcctgatcta ccgccaccac
    36421 cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact gggcaggatc
    36481 atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca gtatttcacg
    36541 ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca cactgctctc
    36601 cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata aattgttgat
    36661 cgccggtttg tcgccagcgg tactcaaacc accctccttt gagcatcagc agccagggaa
    36721 accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga gtggcatagt
    36781 cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc agttccagcg
    36841 cgatatcacc atctccagca tactcccagt cgatgagttt taaccctgat gcgctatgca
    36901 ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggt cgtggttccc
    36961 gcgctttgcg cagacgtttt aacattcgca gccaacccac agtccgccgc gccggatcgc
    37021 tttgctgcca gtacagttcc agtaagggca acagcgttat tcgccagcca aaacgtggtt
    37081 gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa tacgttttta
    37141 ccgcgccggg cagatagtcg actaccatcc agtcacggag atataaatgc ggcttcggtg
    37201 caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg gactgcggcg
    37261 catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca atgagaaaac
    37321 taccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg
    37381 acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt acctgaccag
    37441 ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc gttaacgttg
    37501 ccagaggcgc tggagtacag cacgtaatga gcgccgacat tgcgggcaat gcctatggct
    37561 ttactacggg tgcctaaact gtcctgcggc gacaaaccta actgttgctt agccatcgac
    37621 agttgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt
    37681 tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct atcaaccagc
    37741 aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat cggctgcatt
    37801 gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgccgttcg atcttcgtgc
    37861 tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt
    37921 tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc aggttcacgt
    37981 tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt
    38041 tttgtcatca agattcaccc ctttacaaat atagataaag tctgactttg tgcgccccca
    38101 gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc gggaatggta acgctgcgtg
    38161 gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa cgataatgaa
    38221 cggtaacggg ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt tgaatatcag
    38281 acgtcgaaag gacgggcttt tctgcactga tgcccgcagc caataaagat gactccatca
    38341 ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc accagcagta
    38401 acaacaccag agacaccagc ccaaagcatc cttttctcat cgttacagac ctttatgcgc
    38461 cagcattggt cccagcggac ggccacccaa caagtgcata tggatgtggt aaacctcttg
    38521 tccgccatgg cggttggtat tcatgatcag acgatagcca tcttcggcaa taccttcttg
    38581 ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat gctcagctga
    38641 cacgtcgttc acagtcggaa tgaggatatt cggaatgatc agaatatgcg ttggcgcttg
    38701 cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat cggaggggat
    38761 ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt cctttttcaa
    38821 atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag tatggtcgag
    38881 ttaaacctgc tctttcaacc ttaactcacg atttcttaag caaaaaatcg ttttgctgcc
    38941 gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac attctctgtt
    39001 tggtttacac gacaaatatg aatgcgtata tttctcattt gcatttgcaa gcaaaattat
    39061 tcgcacacta aatataaccg ctcatcaccc tcctccggca tgagcctgac gacaacataa
    39121 accaagagat ttcaaatgct ttcaacacaa tttaacaggg ataatcaata tcaagccatc
    39181 accaaaccgt cactacttgc cggttgcata gcactggcac tattaccttc tgccgctttt
    39241 gctgcaccag ccactgaaga aacggtgatt gttgagggtt cagccacagc tccagatgat
    39301 ggcgaaaatg attacagcgt aacgtctacc tctgcgggta ccaaaatgca gatgactcaa
    39361 cgtgatattc ctcagtcggt cactattgtt agccagcagc ggatggaaga tcagcagtta
    39421 caaacgctgg gcgaagtgat ggaaaacacg ctggggatca gcaaaagtca ggcggattcc
    39481 gatcgtgctc tttattattc ccgcggattc cagatcgata actatatggt tgatggtatc
    39541 cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac tttctgatat ggcactgttt
    39601 gaacgcgtag aagtagtgcg tggcgcgaca ggactcatga ccgggacggg taatccatct
    39661 gcggcaatta atatggttcg aaaacacgcg accagtcgtg aatttaaagg cgatgtctcg
    39721 gcggaatacg gtagctggaa caaagaacgg tatgtggcgg atttacaaag cccactcacc
    39781 gaagacggta aaatccgcgc gcgaattgtc ggcggctacc agaataacga ctcatggctg
    39841 gaccgctaca atagtgaaaa gaccttcttc tcgggcattg tcgatgctga tttaggcgat
    39901 cttacgacgc tttcagccgg ttacgaatat cagcgcattg atgttaatag cccaacctag
    39961 ggcggtttac cgcgctggaa tactgatggc agcagcaaca gttacgatcg cgcacgcagt
    40021 accgcacctg actgggcgta caacgataaa gagatcaaca aggtctttat gaccctgaag
    40081 cagcggtttg ctgatacctg gcaagcgaca ctgaatgcca cccactctga agtcgaattt
    40141 gacagcaaaa tgatgtatgt cgatgcctat gtaaacaaag cggatggtat gctggttggg
    40201 ccatacagta attatggacc tggctttgat catgtcggcg gcaccggttg gaacagtggc
    40261 aaacgtaaag ttgatgcgct ggatttgttc gctgacggta gttatgaatt gtttggtcgt
    40321 cagcacaatc taatgtttgg tggcagttac agcaaacaaa acaatcgtta cttcagttca
    40381 tgggccaaca tcttcccgga tgaaattggc agtttctaca actttaatgg caatttccca
    40441 caaaccgact ggtcaccaca gagcctggcg caggacgata ccacacatat gaaatcgtta
    40501 tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc tgatcctcgg cgcacgttat
    40561 accaactggc gggttgatac gctgacttac agcatggaga aaaaccacac cacgccttac
    40621 gctggtctgg tgtttgacat caatgacaac tggtcgacct acgccagcta tacctctatt
    40681 ttccagccgc aaaatgatcg tgacagttca ggcaaatatc tggctccaat caccggtaac
    40741 aactacgagc tgggtctgaa atcggactgg atgaatagcc gtctgaccac cacgttagcc
    40801 atcttccgta ttgagcagga taatgtcgct cagtccaccg gtacacctat ccccggcagc
    40861 aacggcgaaa ccgcctataa agcggtggat gggacagtca gtaaaggggt ggaatttgaa
    40921 ctcaacggcg caattaccga caactggcag ctgacatttg gcgcaacgcg ctatcttgca
    40981 gaggataacg aaggaaacgc cgttaatcct aatctgccac gcaccacggt taaaatgttc
    41041 accagctatc ggttgcctgt catgccagag ttgacagtcg gcggtggtgt taactggcaa
    41101 aatcgcgtgt ataccgacac cgtgacaccg tatggcacct tccgcgccga gcaaggtagc
    41161 tacgcgctgg tggatctctt cacccgctac caggtgacga aaaacttctc gttacagggg
    41221 aacgtcaata acctgttcga caaaacctac gataccaaca tggaaggttc tatcgtctac
    41281 ggcgcaccgc gtaatttcag cattaccggc acgtatcaat tctgaataac acctgtaaaa
    41341 aaggcagcca tctggctgcc ttagtctccc caacgtctta cggattagtg gttacggatg
    41401 tactcatcca tctcggtttt caggttatcg gatttagtac cgaaaatcgc ctgaacacca
    41461 gaaccagcaa ccactacgcc cgctgcgccc agtttcttca ggccagcctg atccacttta
    41521 gacacatcag caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt
    41581 tctttaccac caaatgcagc aaccagagcc ggtgccattt cgctggtacc tgttgctttt
    41641 gcatcttcag tcgcgtcttc acgacccggc gttttcagat ccagtgcttt aatcagcacg
    41701 cggaagatgg tgtagtaaac aatcgcataa ccgataccga cgatcgggaa cagccacagt
    41761 ttgctgctgt taccagacag aacgatgaag tcgatcagac cgtgcgagaa cgacgtaccg
    41821 tcacgcattc ccagaagaat acagattggg aatgccaggc ctgccagaat cgcgtggatg
    41881 atgtacagga tcggcgcaac gaacatgaag aagaactcga tcggctcggt gataccggtc
    41941 aggaacgagg tcagcgccgc ggagatcata ataccgccca ctttcgcgcg gttttctggt
    42001 ttagcagagt gccagatagc aattgcggca gctggcagac cgtacatttt gaacaggaag
    42061 ccaccagaca gtttacccgc agtcgggtca cccgccatat aacgcggaat gtcgccgtgg
    42121 aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg tacgttccag
    42181 atgtggtgca gaccaaacgg taccagacaa cgttcgatga aaccgtaaat gccaaacgca
    42241 actaccgggt tctggtaagc agcccactga gagaaggtct ggattgcaga accaatcggc
    42301 ggccaaatga aggacagcac aacgccagta aagatggcag ccaggccaga aatgatcggc
    42361 acaaagcgtt taccggcaaa gaagccaaga tactcaggca gcttaatacg gtagaaacgg
    42421 ttaaacatgt acgctgcaat cgcaccggag ataatccctc cgagtacgcc agtatccgcc
    42481 aagtgtttag aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc
    42541 atggttttaa ccatgatgcc ataggcaaca actgcggcca gcgcggatac gccatcgtta
    42601 ttggtaaagc cgagggcgac accgatcgca aaaatcagtg gcatgtttgc aaagacggaa
    42661 ccgcctgctt ctgccataac atgcgataca acggcgggca gccagctgaa attcgcggaa
    42721 ccgacgccca gcagaatacc tgcgataggt agtacggata ccggcagcat cagcgattta
    42781 ccgacctttt gcaggttagc aaatgcattc ttaaacataa ttgagagtgc tcctgagtat
    42841 aggtgctttt tttacgttct cacgcgtggc aaggggggag agcctcgccg tgtacagggc
    42901 atctaagcgc cctttattta ttacacagag taaaataatt cagtgccaat ttgtttgacg
    42961 gctatcacgt ttcaacttca gatcattgga gaagttgcac attttgacaa aacatatgtc
    43021 acaatatttc aaacgcggtt attcaccgcc caattttcgg cggtgaactt tactcgtttt
    43081 agccattaat tacgagcttt aaaaaaactc attcaacgga tggattgaag gcgggaagcg
    43141 tcgatgtgaa acagacgggc gaagttatcg gtggttacct gcgccagttc ttcaacggca
    43201 acacctttca acacagccat gtattctgca acgtcacgaa ccatcgcagg ttgattctct
    43261 tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag tttccaccag taatcgatcc
    43321 agggggacat aacgcgcagc atcgcacagt tgctccgcat tacggaaggt cacaatgccg
    43381 gaaaaggaga tgtaaaatcc gagatccagt aatttacccg ccgtttctct gtcctctgta
    43441 aaacagtgta gtacgccacc gcaatccgtc actttttctt cgcgcagaat tgccagcgta
    43501 tcggcacggg cgtcacgggt atggacgata accggcttgt tcagttcacg accaatctgg
    43561 atatgatgga tgaaggactc ttgctgacgt actttagttt ccggtgtgta ataataatcc
    43621 agcccggttt cacccagcgc tacaacaccc tcttctgccg ccagacggcg taaatcttct
    43681 acatcgtagg gatcattctg gttgagtgga tgcacgccac aagaaaatac gacgttgtca
    43741 cgttcgccta ccagatcccg catatgtaaa taacccggta atgttgtggc gactgccaga
    43801 caaaatttca catcgcgtgc ggcggctttc gccagaacgt catccacgtc cttatgcaaa
    43861 gattcataat ccagaccatc gagatggcag tgtgagtcga ctaaaaacat gatgtctctc
    43921 ttaaagatga ggaaccggta gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa
    43981 aagatcggtg atgagaagct cgcggttgat gcctgtgaca gacattaact gttcacgaat
    44041 gtggcaaaca tcccccagta tagcctgcag gcgcgaggga gaaagatggt ttgccagttc
    44101 ggcgaccagg cccggcacat caacattggt caccagcgca gcgccatgat ggcgttttag
    44161 cgcatccatc agcaacgttg ccacccagtg taaacgcgcc ggagcttgtt catgattaag
    44221 ggccgccagc agcgaatacc agtcgcccga tggcacgcta tatgccaacg cctgacacaa
    44281 tgtttcacga gcctgccagt tatctccctg aaacaacgcc agtgccgcgc caggcgaacc
    44341 ggcgcttaag cgcaatgcgg caagtaatgc atcctgtgac attgtcactt cgcgtgaaag
    44401 ccaagtcacg gcgtactgtt ctggaggcgg cgcaaggtaa tgtaaccgac aacgactacg
    44461 taatgttgcc agtaaacgtt caggctcgcg ggtagccagg aaaaaccagg tttctgctgg
    44521 tggctcttca agcgttttca gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc
    44581 ggttacccag acaactttcg caccacctag gcgtgcgtgc tcattcagct tttcggtgac
    44641 ctcacgtacc gcatcaatgc ccagcgtatt tttccctttt tcgggagcca gggtgtagta
    44701 atcgggatgc gtgccagcct gcatcaactg acatccacga cagtgaccgc aacttttgtg
    44761 gccctgcggt tgttggcaga gtaaataacg gctcagggcg tagattaaag catcatcgcc
    44821 catgcccggt aacgcctgaa tgagtagcgc atggtgacct cttccggcct gatagctggc
    44881 taccagtttt tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc caactccttc
    44941 acccagtggg tcacggtagt gcggattgca tccatcacgg cctccagcgg ctgggtggca
    45001 tcaatggtat gaatactttt atcttgtgca gccagttcca gatagcgggc gcgggtgcga
    45061 ttaaagaaat cgaaagactc ttgctcaata cgatccagct cgccgcgcgc acgcgcgcgt
    45121 tttaagccaa cttccggggt aacatcgaga tagagcgtta agtcggggcg aaaatccccg
    45181 agaacagcat cacgcagtgt tgccagcata tgttggtcaa taccacgtcc tccgccctga
    45241 tacgcctgag tggagagatc gtggcgatcg ccaatcaccc aggtgccgtt agccagcgca
    45301 ggtttgatga ccgtttctac cagttgaacg cgcgcggcat aaaacatcag aacttcggct
    45361 ttatcggtaa tgacttcatc gcctaccgat ttgatatcca gcaccaggct tcttaacttt
    45421 tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat acccagttgc
    45481 tcgagcgtct caaccaccac attacgcgcg gtagttttgc ctgcgccttc cagcccctca
    45541 atgacgatat acttactgcg catttttttc cttaagcact ttcagataat cctgcacaga
    45601 cttgttatga ctggcaagat tggtattaaa cgtgtgacca cctttaccat cggccacaaa
    45661 atagagatac ggcgtttttg ccggatgcgc agcagccttc agcgaatccg cccccggcgt
    45721 agctatcgca cccggcggca aaccggtaat ggtataggtg ttatacgctg tcggcgtttc
    45781 caggtctgca cgagaaagtt tgccattata acgctctccc atcccgtaaa tcacggttgg
    45841 gtcggtctgc aggcgcatac caatgcgtaa acggttgata aataccgagg caacctgatc
    45901 gcgttcactg gcaacggcgg tttctttttc gataattgat gccatcgtca ccagctgatt
    45961 tttatcttta taaggcagac cgtccgcacg cccttcccag gcgctatcga ccgctttcac
    46021 cattttcttg tgagctcgct tgagtaacgc gacatcggtg gtattggcgg tatacatcaa
    46081 ggtgtctggc cagaaccaac cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc
    46141 tacggtggcg tacttatcgt cactcagcgt gtgcttgata tacggagcct cacgcaattg
    46201 cttgaggtag tcgctcagac gcatcccttc taccagtcgc agcgggaact gtgcttcttt
    46261 accgctttcc aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt
    46321 cccggcttta aagtgagaaa gatccggttc gatacgcagc agccattgaa aaacccgtgg
    46381 gcgattgatg atcttatcag cataaagctg ttcaccgagc gccagacgtc cggtccctgg
    46441 cttcagggta aatatcgtct cttctttgat aagcaatttg ctgtcggcaa gatggcgaac
    46501 cttccagacg cccacaccag cggcgatacc cagtaccacc agcaataaca agataatcaa
    46561 taacactttt ttcatgacta attcgggcgc tcacaaagtg gggctaaata ttcatataac
    46621 gttgctgacg aaaaggtgac atcgccacag gcacgtacgg gcatcactgg cattaacgca
    46681 ttacaaataa ccatctcatc tgcctgcaac gcctcctcca gcgaggcttg cacttcgaca
    46741 agctgataag aggattgtgc cagtaaacgg atacagaatt gtcgcataat gccgttaaca
    46801 cctgcctgat ccagtcgcgg cgtataaact acattgccct tccgccagaa caaattagcc
    46861 gcacagcatt ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc
    46921 tgctcaagat gagagcgaat caatacttgc tcaagccgat taagatgttt aatacctgca
    46981 agatgaggat tgcgccccag ccgcaccggg cttagcgcca acgtcatccc ttcgttacgc
    47041 aaacggtcgt aatgtgcagg ataagccgta acggagagaa tccgcgttgc tggtccgctg
    47101 ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga tcacgacttt cagcacacca
    47161 ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa gctgaggcca gaagtcacag
    47221 gaaatcatca accgctgaca agcatcctgt agtcgctgga tatgcgccga taacaaactg
    47281 actttaccgt cgataactct ggcggtggta aaacaaccat caccaaactg cgttgcccga
    47341 tcgcttactg ccagcgattc ctgcttataa ccgttaatta agaacatacc ggctccttat
    47401 cagagaattg ttagtgtggc agcatgttca ctacggaaca agtcggaata aaagctaaga
    47461 aaaaaggccc gcaagcggac cttttatatg ggtggaaaat gacaacttag atctttttaa
    47521 agatcaaaga accattagtg ccaccgaagc cgaaggagtt acacagcgtg tattccattc
    47581 cgctgacctg acgtgcttca tgcggtacga aatccagatc gcaaccttca tccgggttat
    47641 ccaggttgat ggttggcgga acagcctgat cgcgcagcgc caggatggag tagatagatt
    47701 ctactgcacc cgccgcacct aacaggtgac cggtcataga tttcgtggag cttaccaaca
    47761 cacggcttgc agcttcaccg aagatggttt tcaccgcctg cgcttcagct ttatcgccag
    47821 ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg actcgcttca atgcctgcat
    47881 cacgcagagc atttgccatt gccagagctg cgcctgcgcc attttctggc ggtgacgtca
    47941 tatgataagc atcgctgctc ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc
    48001 gttttttcgc gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg cccagtacga
    48061 agccatcacg ctctttatcc cacgggcggc tcgccgcttg cgggttatca ttgcgggtag
    48121 ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg cgtactggct ttctctgcgc
    48181 cacctgcaac catcacgtca gcatcgccat acgcgataat acgcgcagca tggccaatgt
    48241 tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct tgggccacgc aggccataca
    48301 tgatagtcag atgacctgcc accatgttca caatcgttga cggaacgaag aatgggctga
    48361 tcttacgtgg accaccgttc atcagagatg tgtggttttc ttcgatcagt ccgaggccgc
    48421 caatcccgga gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc
    48481 cagaatcctg catggcctga acgccagcga caattccata ttgaatgaag gcatccatct
    48541 tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa atcctttact aagccagcaa
    48601 atttcgttgc ataggcgcta gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg
    48661 caagcagagc tttccaggta gactctacgg tattgccgac aggagacaac atgcccagtc
    48721 cggtcacaac tacacgacgc ttagacacgt ttgtcctcca aggagggaaa aatgattcta
    48781 gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc
    48841 ctggtggccg ttgatgtaat caatggcagc ctgaacggtg gtgattttct cagcttcttc
    48901 gtccggaatc tcagtatcaa actcttcttc cagagccatt accagctcaa cggtgtcaag
    48961 agaatccgcg cccaggtctt caacgaaaga agcattgttg gtaacttctt cctgcttaac
    49021 gcccagctgt tcgccgataa ttttcttaac gcgttcttcg atagtgctca tactcttaaa
    49081 tttcctatca aaactcgctt tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt
    49141 gcaactaaat cccggcaggt cttaccacga ttttacgtta ttttgaggcc ttacccccca
    49201 ataacgcaaa tcattttgca ctaattatta ctcataacca cgcagactgc gcgcaacatg
    49261 agcattttgt gcaaatcgcg gtcagaccat gtacatcccg ccgttcacat gcaaagtttc
    49321 acccgtgatg taagctgctt cgtcggatgc caggaatgca accgcgttgg cgatttcctg
    49381 tgcgccgccg aggcgacccg caggaacctg cgccaggata cccgcacgct ggtcatcgct
    49441 cagcgcacgt gtcatgtccg tttcaataaa gcccggagca acaacgttta cagtaatacc
    49501 gcgtgacgca acttcgcgcg ccagtgattt actgaagccg atcaagcccg ctttcgccgc
    49561 agcgtagttg gcctgaccgc catttcccat ggtaccaacc acagaaccga tagtgataat
    49621 acgaccatga cgctttttca tcatagcgcg cattaccgct tttgacagac ggaaaacaga
    49681 tgaaaggtta gtttcgataa tatcgttcca ctcttcatct ttcattcgca ttaacaggtt
    49741 atcacgagtg ataccggcat tattgaccag gatatccact tcaccaaatt ctgcgcgaat
    49801 tttttccaga acagattcga tagatgccgg gtcggtcaca ttcaacatca gacctttgcc
    49861 gttggcacct aaataatcac tgatcgcctg agagccattt tcactggtcg cagtgccaat
    49921 aactttcgcg ccacgggctg cgagcgtttc agcaattgcg cggccaattc cgcggcttgc
    49981 accggttacc agtgcgattt ttccttcaaa attcatgatt ttcctctttt aaagctcgag
    50041 cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg
    50101 tttcgtcagg ccagtaagca ccttgcccgg gccgacttca tagagatgtt ctacgccttg
    50161 cgctgccatg tactcaacag acttcgtcca ctgaaccggg ttatacaact gacgtaccag
    50221 tgcgtcacgg atggcatcac cattggtttc gcatttcaca tcaacgttat tcacaacaga
    50281 aactgttggt gcgttaaagg tgattttcgc taattctact gccagtttgt cggctgctgg
    50341 tttcatcagc gcacagtgag acggtacgct cactggtaac ggcagcgcgc gttttgcgcc
    50401 agcagcttta caggcagcgc cagcacgctc aaccgcttct ttatgaccgg caataaccac
    50461 ctgtcccgga gagttaaagt ttaccggaga aacgacctga ccttctgcag cttcttcaca
    50521 cgctttcgca atagacgcat catccagacc gatgattgcc gccatagcgc ccgtgccttc
    50581 cggtacggct tcttgcatga acttaccgcg catctcaacc aaacgcaccg catcagcgaa
    50641 atcaatcacg ccagcgcaaa ccagcgcgga gtattccccc aggctgtgac cggccatcat
    50701 tgccggtgct ttaccgccct gctgctgcca tacgcgatac agcgcaacag atgcagtcaa
    50761 cagcgctggc tgggtttgcc aggttttatt cagttcttca gctggcccct gctgggtcag
    50821 cgcccacagg tcgtagccca gcgccgcaga agcttcagca aacgtttctt cgacaattgg
    50881 atagctcgcc gccatatcag ccagcattcc aacggtttga gaaccctgtc cagggaacac
    50941 aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa cgaaccagcg cggagcccca
    51001 agtgaatcca ccgccaaagg cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc
    51061 gcgtacagct tcatccagcg cgcacgggac agaggccgca gaggtattac cgtggcgatc
    51121 cagcgtcacc acgacattgt ccatcgacat accgagtttt ttcgccgttg cactgataat
    51181 acgcaggtta gcctgatgcg gaaccagcca gtccagttga gaacggtcaa gattattcgc
    51241 cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc gcaaccttga agacttcgtt
    51301 gcccgccatc gtcagatgaa ttgaattctc tggattcacg cggtcggcgt ttggcagcgt
    51361 cagcaattca ccataactac cgtcggcatg cagatgggtg gaaatgattc ccggctcttc
    51421 agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa ataataatag tcccacgatc
    51481 tgttggatcg caggtgcgcg ccagtacatc ggaaccgacg accagagcat acttcaccgc
    51541 cccagatttc acgtattgat cggctacgct taatgcatag gtgaaacctg cgcaggctgc
    51601 tgcaacgtca aatgccgggc aacctttaat gcccaacatg ctttgaatct gacaagctgc
    51661 gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg atcaggccaa tctggtcttt
    51721 ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt
    51781 ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta cgagtgacaa tccactcgtc
    51841 agaggtgtcc accatttttt ccaaatcggc gtttgtccgc acttgttcgg gcagatagct
    51901 gccagtacca ataatcttcg tatacatgta cgctcagtca cttttcggtt atataccgtc
    51961 acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg cagagttccg cttttgccac
    52021 cgtccagcag ctcaaaacca gctgggtata cagattccag gcgagcggca attcgctgag
    52081 gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat
    52141 tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc taacagacag gcgccgttat
    52201 actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta
    52261 ataacagtag ccaccacgac cgttttttcc cttcaccctg agatttcagc agagaaagga
    52321 acatcctgac aacaccttcc atcgtcttta acgtgacatt tcctgtaaag ccgtcacaaa
    52381 ccagcacatc tgtcttgcca gttaacaact cattggcttc aagatagccg atataattga
    52441 tagaagggat tgttttaagc accgctgagg catcccgaat actgtcaaga ccctttactt
    52501 cttcttcacc aatattgagc aacgccacgc gaggattggg aatttccacc acctcttcag
    52561 ccagaactga gcccataatg gcaaattgca ccaacattgt gctgtcacaa tcgacgttgg
    52621 cccctaaatc gaggaccacc gttttgccct tttgctgatg tggtaatacc gtcaccagcg
    52681 ccggacgctc aatcccctcc aggggcttga gtaataattt tgccagcccc atcagtgccc
    52741 cggtattacc ggcactgaca caggcttgcg ctcgaccttc tttcaccagc tccagggcca
    52801 tacgcattga actcccacga ctggcgcgga tagcttgcga aggccgggca tcactggcga
    52861 taactgactg cgcaggaata atctgcagac gcgaacgttg ttcaaagtca gctttagcaa
    52921 gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag agtgagttgc gaattagaat
    52981 tcagtgcctg caatgctgca ggcactgtca cggaagggcc aaaatcccct cccatgacat
    53041 ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt
    53101 tgcctgggga aaatcctcac taagcttcat cacgcagacg cgtgattact tagcgatgac
    53161 cttgcggccg cggtagtaac cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga
    53221 agttttgtct acagacaggc tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc
    53281 acgtttggaa cgggttggtt tattctgttg tacggccatg gaccttactc ctcaattacg
    53341 gtttatcccc gatccaacgg ggagcaccaa ttacttacgc tttaagctgg ctaatacggc
    53401 aaatgggttt ggcttttgca cttcttcagg cagttcacca aagaccatat ccgcttcgga
    53461 cacttcacag tgttcagaat catgcaccgg aactaccggc aaggcgagga tgatttcatc
    53521 ttcaaccatt gcaagcagat cgatttcacc gaattcgtta acctcaatcg gttcatacgc
    53581 ttccggcagt gcttcagcct gttcgtctga acgcacagga ctaaaacaat acgttgtgta
    53641 gacctgatga gtaaacggct tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc
    53701 atcgccgttt aacactgcga gacgttggtt atcgatagcg aacgacatgg agcattccac
    53761 atcactgtcc acactgacta cggattcggc gacgcgctca acctgatcag gggtatagat
    53821 accctggtaa tcaaggcgtt tttgagccgt acgaaccgga tcgagagtca gggggaattt
    53881 taccttttgc atagggcgcg catattaact ttgtaacgtc atagagtcaa agaaaaaggc
    53941 agcctgtggt tgccttttgc caataattcg cacacattgc gggttactgc tttattttcg
    54001 ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg catagtttaa aatggctatc
    54061 atcaatacgc tatatctgat ggaaaaaaat atgcctaaac ttattttagc ctccacctcg
    54121 ccatggcgtc gcgctcttct ggaaaaactg caaatctctt ttgaatgtgc agcacctgag
    54181 gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt tggtgcttcg actggcacaa
    54241 gaaaaagcgc aatctctggc gtcacgttat ccggatcatt taattattgg ttcagaccag
    54301 gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg
    54361 caattacgca aagccagcgg caatatcgtc accttctata ctggactggc gttgtttaat
    54421 tcggcgaatg ggcatctaca aacagaagtg gagccttttg acgtccattt ccgtcaccta
    54481 agcgaggcgg agattgataa ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc
    54541 tttaagagtg aaggatttgg cattacgctg tttgagcgtt tagagggacg tgaccctaac
    54601 acgctggttg gtttaccgct aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac
    54661 ccgctgatgg gatagttgtt tgcgccggat gcggttacgt cgcatccggc aatcggtgca
    54721 ggcggcctga tgcggcgtaa acgccttatc aggccaaccg cccattatca ggcttatatg
    54781 cttgtattag cgcgcgttac gcagtttttg caaacaacgc ttcaaacctt catccatcgg
    54841 cgcttcgata cgcatcacct caccggtccc cggatgggta aacttcaacg ctgcggcgtg
    54901 caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc
    54961 accgtagcga tcgtcaaagg caatcggatg acccgcatac tgtgtatgca cacggatctg
    55021 atgagtacgt ccggttaccg ggctacaacg caccagggtg gcaaatgcat agcgttcttc
    55081 cactttaaag cgtgtttccg acggtttgcc ttcctgactg acacgcacga tacgttcgcc
    55141 gctttgcaga atatttttca ataacggcgc ttgaacgctc ttcacatgcg actgccactg
    55201 accgcgcacc agcgccaggt aatctttttg cattcctttt tcacgtaatt gctcatgcag
    55261 agaacgcaac gccgagcgtt ttttcgctac cagcaacaca cctgaagtgt cccggtcgag
    55321 acgatgaacc agttcaagga accgcgcttc cgggcgcaac gcccgcaaac cttcgataac
    55381 gccgaagctt aaaccactgc cgccatgtac cgccgtaccg gaaggtttat tcagcaccag
    55441 gatatgatca tcttcatata agatgacgtc cgccagcgcc gccacctttt gcagatgtgg
    55501 cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt gggatacgca cctcatcacc
    55561 cgcttcgagt ttatattcag gcttaatacg ttttttgttc acccgcactt cacctttacg
    55621 caaaatacgg taaatcatac tttttggtac gcctttcaat tgggtacgca aaaagttatc
    55681 gatacgttgc cccgcttcgt cagcggtgat agcaacaatt tttacggatg gagtctctgt
    55741 tttcatggtg ggcgattcta attagccaac aggattcgcg ccactcattt ttctatgctt
    55801 atatttagtt ttgcacctta ttacttcact acgtgatcac tttattgatg gttattaaac
    55861 caatcaccag caagaagtga aaaaactgtg agtaagcggg tgataaatgg taaaagtcat
    55921 cttgctataa caaggcttgc agtggaataa tgaggccgtt tccgtgtcca tccttgttaa
    55981 aacaagaaat tttacggaat aacccatttt gcccgaccga tcatccacgc agcaatggcg
    56041 taagacgtat tgatctttca ggcagttagc gggctgcggg ttgcagtcct taccggtaga
    56101 tggaaatatt tctggagagt aatacccagt ctgtttcttt gataattgcg ctgtttttcc
    56161 gcatgaaaaa cgggcaaccg acactctgcg cctctttgag ctgacgataa ccgtgaggtt
    56221 ggcgacgcga ctagacacga ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc
    56281 agcttagtcg tcaatgtaag aataatgagt aagttacgat gaaaagaatg ttaatcaacg
    56341 caactcagca ggaagagttg cgcgttgccc ttgtagatgg gcagcgtctg tatgacctag
    56401 atatcgaaag tccagggcac gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc
    56461 gcattgaacc gagtctggaa gctgcttttg ttgattacgg cgctgaacgt cacggtttcc
    56521 tcccactaaa agaaattgcc cgcgaatatt tccctgctaa ctacagtgct catggtcgtc
    56581 ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat tgttcagatc gataaagaag
    56641 agcgcggcaa caaaggcgcg gcattaacca cctttatcag tctggcgggt agctatctgg
    56701 ttctgatgcc aaataacccg cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc
    56761 gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg
    56821 tgcgcaccgc tggcgtaggc aaatctgctg aggcgctgca atgggattta agcttccgcc
    56881 tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg cccggctccg ttcctgattc
    56941 atcaggagag caacgtaatc gttcgcgcct tccgcgatta tttacgtcag gatatcggcg
    57001 aaatccttat cgataacccg aaagtcctcg aactggcacg tcagcatatc gctgcattag
    57061 gtcgcccgga tttcagcagc aaaatcaaac tgtacaccgg cgagatcccg ctgttcagcc
    57121 actaccagat cgagtcgcag atcgagtccg ccttccagcg tgaagtccgt ctgccatccg
    57181 gtggttccat tgttatcgac agcaccgaag cgttaacagc catcgacatc aactccgcac
    57241 gcgcgacccg cggcggcgat atcgaagaaa ctgcgtttaa caccaacctc gaagccgccg
    57301 atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg cctgattgtt atcgacttca
    57361 tcgacatgac gccagtacgc caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc
    57421 gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg ctttggcctg ctggaaatgt
    57481 cccgccagcg cctgagccca tcactgggtg aatccagcca tcacgtctgc ccgcgctgct
    57541 ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct ctctattctg cgtttgatcg
    57601 aagaagaagc gctgaaagag aacacccagg aagttcacgc cattgttcct gtgccaatcg
    57661 cttcttatct gctgaatgaa aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg
    57721 gtgttcgctg cgtgattgtg ccaaacgatc agatggaaac cccgcactac cacgtgttgc
    57781 gcgtgcgcaa aggggaagaa acgccgacct taagctacat gctgccgaag ctgcatgaag
    57841 aagcgatggc gctgccgtct gaagaagagt tcgctgaacg taagcgtcct gaacaacctg
    57901 cactggcaac ctttgccatg ccggatgtgc cgccagcgcc aaccccagct gaacctgccg
    57961 cgactgtcgt agccccagca ccgaaagctg caacggcaac accagcagct cctgcacaac
    58021 ctgggctgtt aagccgcttc ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag
    58081 ccaaaccgac cgagcaacca acaccgaaag cagaagcgaa accggaacgt caacaggatc
    58141 gtcgcaaacc tcgtcagagc aaccgccgtg accgtaatga gcgccgcgac acccgtagtg
    58201 aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc
    58261 agcaaactgc cgagacgcgt gagagccgtc agcaggctga ggtaacggaa aaagcacgta
    58321 ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac
    58381 gtcaggcgca acaagaagcg aaggcgctga atgttgaaga gcaatctgtt caggaaaccg
    58441 aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga
    58501 aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa
    58561 ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc acgcacagaa ctggtgaaag
    58621 tcccgctgcc agtcgtagcg caaactgcac cagaacagca agaagagaac aatgctgata
    58681 accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga
    58741 gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt
    58801 tgaccgtagc gtgcgcgtct ccggaactgg cctctggcaa agtctggatc cgctatccaa
    58861 ttgtacgtcc gcaagatgta caagttgaag agcagcgcga acaggaagaa gtacaagtgc
    58921 agccgatggt gactgaggtc cctgtcgccg ccgctgtcga acctgttgtt agcgcgccag
    58981 ttgttgaaga aatggctgaa gtcgtagaag cccccgtacc ggttgccgaa ccgcaaccgg
    59041 aagtggttga aacgacgcat cctgaagtga ttgccgccgc ggtaactgaa cagccgcagg
    59101 tgattaccga gtctgatgtt gccgtagccc aggaagtcgc agaacacgca gaaccggtgg
    59161 ttgaaccgca ggaagagacg gcagatattg aagaagttgc cgaaactgct gaggttgtgg
    59221 ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag
    59281 aagttgaaac ggtagccgcg gttgaaccag agatcaccgt tgagcataac cacgctaccg
    59341 cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt cacagtgact
    59401 ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat acggcaacgc
    59461 atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta gctcaaagta
    59521 atcaagccct ggtaactgcc agggcttttt tatttcatgt ttgaatcaac atattaaaat
    59581 tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaaa tgcccgcccc
    59641 ggcgatccca gagcgggcag acaaaacata cccagtttca tgatatgttt taaagcgagc
    59701 ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt tatacgatgc
    59761 ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt ccacatcaac
    59821 cagatcgctc atctgctgcg tttgccctaa agcgcgatcg ctacctaagg aatccagcga
    59881 ctccagtttg ttcagctgcg tgcctaattc cgtgcgaacg gtaaggacat tattcagcga
    59941 gtttttcagt ccacggttgg ttttatccaa tgccgcagcg gcgatttctt tatccgcttc
    60001 gctatccgcg accggcgttt tcagggctgc gatggcgcta tccagcatgg caaaaagatt
    60061 ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta ctggtaatac tgtcgaaaat
    60121 tttgtcgccc gtatgtccta tcaccatcga acgcgaagca tcgacctgtt gcttaatact
    60181 ttctgttccg ccgacataat caccgttagc ctcactaaac ggcgcagtct ctgttttata
    60241 accggcaaaa atgtagcgcc cattaccgtc agtggtgttc gccagattca gcaactggtc
    60301 acgaagcccc tgaatatccg tagccagcga ggcccggtca tcgtcactca aggtgccatt
    60361 gctggcgtag acaatttttt cctgagcatt ctggatagca gtggtgacct ggctaagtac
    60421 actctcttcc agtgacactt tttgagtggc gaaagtacgt gccagcgtgt actggctgtt
    60481 ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca gcaatgggat cgtctgaagg
    60541 gttaacgact cgcttacccg tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga
    60601 attggtgatg ccgcgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc
    60661 tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc
    60721 gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc cgtattcctc atcgagattg
    60781 acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg
    60841 ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg
    60901 tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc tttgcagatc cagcagggcc
    60961 tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc gattttcgct
    61021 tcgtcggtga ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag
    61081 ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta
    61141 ccgttggcat ctggtgtcgc cgtaaaagtg gtattgctgg caaggcgggt gacctgccac
    61201 tgattattat cgaacgagat tttgtaatct gtcgccagta ccacggaggc atcagttacc
    61261 gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt gctgaaccgc gggattaccg
    61321 atagtaaaga aatcttcacc agcatcgccg ttagcatcaa atccggcttt gtgttggctg
    61381 ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt ctggtccaga
    61441 tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagtaa tttctccggg
    61501 atctcaatat tgcctgccgt cccatcaacg taagcgacag tcgtacgaga agggtcggcg
    61561 ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg
    61621 gcatcgtgat gttataagta ccgccatcct gaacgctgac ttctacgcca acaatctggt
    61681 ttaattcgct caccagttga tcgcgttgat ccagcagatt gttaggtgac gcccctgccc
    61741 ccacgcctgt caggcgcgag atttgatcgt tcaggctggc aatttgttta gcgtaattgt
    61801 tgatctgatc aacgctggca cctatcgcga tattgacctg tttgtcctgg tcgcgcagat
    61861 attgatcggt ggttttaaac tgattcacca acccttctga tttcccaatc agcgcctggc
    61921 gcgccgccgg gtcttccgcg ttactcacca gcgtttgcag gctggtgaag aaatcctgca
    61981 tctgtgttgc cagcgaagag gtactggtgg agagcatatt gtcgattttc gacatctgct
    62041 catagcgggc ggtcaggccg ctactttgcg tctgcacagc acgtaactgg ttggtaataa
    62101 acgtatcata ctcacgctgc acaccagaaa cgtagacgcc attgccaacc cagccgccag
    62161 cgcccaacgt gctattggcc tgcgccataa tagtggtttg gcgggtatat ccggcaacgt
    62221 tatagctgga gatattatta cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc
    62281 tcatggcgtt attgatcaag ctggacatgg aggttcctta taagccttca attacagagt
    62341 attatcggca gcgacccgcc ggacttgagt tattcagaac agattatcaa tgttcatgct
    62401 gtaggttttg ctcacctggt cgcttatcga tttcatctgc tggatcatgt tggtgagttt
    62461 acgggcatag tgaggatcgg tagcatagcc cgcgtcctgt aggacctgcg ccccctgttc
    62521 cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta
    62581 atccgacaat gcttccagat acgagctgta gacccgaaac ttcgctttta ctttcttcgc
    62641 ttcgccgttt tcatattcag tcgtggtgat ttcagtgact tgccctttcc agttgccaga
    62701 ggctttgaca ccaaacaggt tatagcttgg ctcgccgttt tcgcggcgga tttgccgttg
    62761 cccccaacca gattcaagcg cagcctgagc gaggatcaaa tgatgtggca caccgctttg
    62821 ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg aatgctctac tgtcacccgg
    62881 cagcgaatca tcgtagttac gtggtacggc cttttgcacc agctgcgaaa gcgtctggtt
    62941 ttgataacgc accacggttt cgagcgggaa tttcatcggt gctgctggcg tggactcctc
    63001 tggcaatggt tgttctggcg tcatctgttt aaccatcatc tctgcaagcc ccagaccttt
    63061 gcccgccgtc atctgttggg caatttgctg gtcatacata ctggtataca gacgagtgtg
    63121 ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc atgcttttca acatcatctg
    63181 cacgaacatc ccttccacct gacgggccac cggacggata tttgccgccg gatcttcgct
    63241 cgctttcgcc tttagttcgt tgagtgattg cgcatcccag gccgcacttg ccagtagttt
    63301 gctgtcgctg atcatcagat gatttccagt tttgcccgca gacatcccgc actttgcatt
    63361 gattgcaaaa tagacatcag atccatcggc gtagcgccca gcgcattgag cgcacgcacc
    63421 acgttattga ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa
    63481 tcgatctgcg tttgtggagt aacaacagtc tgtccaccac caaacggtgt atctggctgg
    63541 ctgacattgg cctgacgatt aactgttact gagagatttc cctgcgctac cgcgcagctg
    63601 tcgagggtca cttctcgatt catcaccacc gaaccggtgc gcgagttaat cactacttta
    63661 gcgtcctgcg gggtgacatt aacctgcata ttctggatat cggcaaggaa acggacctgg
    63721 gaactgttgc cacttggtac gcgcacctga atagtccgcg catctaacgc ggtggcgctg
    63781 ccatatccac gcacgcggtt gatggtgtca gcgatttgct gcgccatgct gaaatcttcg
    63841 tcgttaagtt gcaaattaag ggtattcccg acgccaaact ggctgggcaa ttcacgttca
    63901 ataaccgcac cattggtgat ccgtccaccg ttcagttggt taacctgaac actgctaccg
    63961 cccgcggagg ctcctgcgcc gccaaccaga atattgccct gcgccagcgc atacacctga
    64021 ctgtcaacgc ccttaagcgg tgtcatcaac aacgtacctc cacgcaagct tttggcatta
    64081 cccatggaag aaaccaccac atcgatggtt tgcccctgac gtccaaacgg aggaagtgac
    64141 actgtcacca ttaccacagc gacgtttttt agctgcatat tggtgcccgt cggaacggta
    64201 attcccagct gtgagagcat gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc
    64261 tggtcaccag tgccatccag ccccactacc aggccatagc caatcagtga gttttgcctt
    64321 accccctgaa cactggtgag atcgcgaata cgctcagcct gagccgccgt cgtaaccagt
    64381 agaagaatta atgcagagag aaatttaatc accacagcct cacttacatt ggcgacaggt
    64441 taaggaagaa acgctgcaac cagcccatat tttgcgcttc gttaatgtag ccattgccta
    64501 cgtattcaat acgcgcatcc gccacctgag tagacggtac ggtattgctg ccgctgatag
    64561 tgcgtggatt aaccacgccc gagaagcgaa taaattcggt accctgatta atggcaatct
    64621 gtttttcacc caccacatgc aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca
    64681 acgtgccgct aaaggtattg ctggcattgg ccccgccctt tccgttgaac gtgttaccac
    64741 cggaggcttc gacatcggca cgagcgttac caaacagccc ctgcaaatag cgcggcacag
    64801 tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt cgcagaggag cttttgctgg
    64861 cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat
    64921 cttcaaacag cggttgatag ccatagttaa tcggctgagc agactggaaa atagaaccgt
    64981 tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc accagcggcg
    65041 tggagggtat ccaggcgcag ccggttagtg aaagcaccaa caagctggaa atggcataag
    65101 tatgcgcagc gtttttttgc atggctatca tcttcaaaaa tcagtaaacc ggtgaacctg
    65161 ccaccggtta tgccttagag ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc
    65221 gctttactgt tgatttcgta agcgcgttgc acctgaatca tattgaccag ttcttccgcc
    65281 acgttgacgt tagacgtttc aacataccct tgatacagca gtcccgcgcc gttcaggccc
    65341 ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt ttcgccaatg
    65401 ctctccagtc cggtatcatt cataaaggtg gtgagattga gctgcccaac ctgaaccgga
    65461 gctgcctggc cttgttgggt tacgctgacc acgccatcac gaccgatggt gatacttaac
    65521 gcattcgccg gaatggtgat cgctggctgc acctgaaaac caccagccgt caccagctgc
    65581 ccgttctgat ccacctggaa agagccgtca cgggtatagg ctgatgagcc atctggcaac
    65641 attacctgga aaaagccctg ccctttaatc gcgacatctt tgctgttatt ggtctgcgac
    65701 agattgccct ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt gccgatttgt
    65761 aatccggagg gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg aatggtttga
    65821 taaagcagat cttcaaacac cgcgcgctga cgcttaaaac cgttagtact gacgtttgcc
    65881 aggttgttgg caatgacgtc catattggtt tgctgggcgt caaggcccgt tttggcgatc
    65941 cataatgaac tgatcatata atgtcctgta gtggattgct gacaagcaga gcagaagcac
    66001 gcgacagtgt gaatttcccc tcaccctaac cctctcccca aaggggcgag ggaatagtcc
    66061 gcgcgctttt attgcaatgt catcaatcgt tcaattaact catcgacagc agttggttgg
    66121 cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca aaacgccgcg
    66181 cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct tccagaaccc
    66241 ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg ccctcgcgtg gcctgggttt
    66301 ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc gtggctttca
    66361 ccagtttcag acgccctact ggcgcaactg tatttgccgg atcgcccgga ttgagcgccg
    66421 agattgtgcc atcggcagca atcgtgattt ccgccccttc cggcacagca attggccccg
    66481 cctcgcctat caccggatgc ccctgaattg tcagttgccc ggtgggatca acctgaatgc
    66541 tgccattacg cgtataccct tcgctgccgt cagcggtctg cacggccagc catcctgctg
    66601 caacgcaacg tccagcgggc gcgaggtgta atccatcttg ccgggcgtca tgtctgcacc
    66661 cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc
    66721 gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag gcattggcca gattactggc
    66781 ggttaccgcc tgttgattca gcgtctggct ggctgctccc atcgcagtat aaattgcgtg
    66841 atccattgag ctatcccgtc agcgattagc gtaagttaac ccgcgtgttg aggatctggt
    66901 cctgggtttt gatggtctgg gcgttagact gatagttacg ctgggcaacg atcatattga
    66961 ccagttcttt actgagatcg acgttggacg cttccagcgc accgttggtc agggtgccaa
    67021 agtttcccgt cccggctgtc cccaacagcg ccacgccaga agattgcgtc gcagaccaga
    67081 cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt acaatctgcc
    67141 ccagcagttg ggtttgttcg ttggaatagt tgccgacaac cgtaccgtca tcattgattt
    67201 gataactcac cagatcgccc ggtttgtagc cattctgggt ggttgccaca atgttgttag
    67261 cgccggtatt ttgctgcatg gagttgagga agctcagact aaacgtggcg ggttctgcac
    67321 cgttaattgc gccggttgcg atattattcg ccatcgcaca tgtgccatta gcattaaatt
    67381 ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact gctatcctgg gtgtagacct
    67441 gccagttatt atccccggtc ttcacaaaat agacgctcat gtcatgagca ttaccctgac
    67501 tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgctg gcgctgaatg
    67561 gcgtaacagt aggaagcgga tcactggaat tcaggttgat ctgcatcgac gccgtggtgg
    67621 tagttttcgc tgccatcagg gtattcggga tcgaaatatt ggtcggattc gccccttgct
    67681 gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag ctgtaaacct tgcatattca
    67741 acaggttacg gttttcatcc agcttaaatt gtccgttacg gctgtagaac accgaaccgt
    67801 tgctgtctac cagacggaaa aaaccgttct ggctgatagc aacgtccaga cctcgcccgg
    67861 tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt acccccagtc
    67921 ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta aagccgtagg
    67981 tggcggagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca gcgtttaatc
    68041 cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct taaattatct
    68101 gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag atccagcgta ttaccgctgt
    68161 tgccgcggat cacaccctgc accagagcaa actgcagcgg ctgggcaacc agttgtgtac
    68221 caccgttact ggcgctaatc gctacattgt aagaaccgtt cggcgcagtg ctgccatcag
    68281 tcaacgtacc gtcccaggtg aaactgtgaa ctccggcggt cagttcacca atatcaatgg
    68341 tgcgcacaac cgcgccattt ttatcggtga tggtggccgt aactttgtct gccgcctgtt
    68401 gcagctcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg ccggttcccg
    68461 caagaacagt tgtgccgggg atcatcacgc cgtgaccgat caggttactg gcctgtaacg
    68521 actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt ttttcaatcc
    68581 cgctgaccgt gctgatttgt gccaattgcg acgtcagctc gttgttttcc attggattgg
    68641 tcgggtccta gtttttcagc tgcgccacca gcaaagtcag aaaactgctt tgtaaatctg
    68701 cggcgttgct gcccgtgagc gaactactgc tggtggtact gacgccggta tttgtcggat
    68761 cggtggtggt taccgcaatg gacatagctt tctcctttat tgaccgagcg taagggtttt
    68821 cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgatagc tgcgtgacgc
    68881 cgacatggtg ttaaccatct ccccgacgac atcaacgttc ggcattttta cgtagccctt
    68941 tgcatctgcc agcggattac ctggttcata aaccagtttg tccggggcct gactttctta
    69001 acatcggcaa cctttacgcc gcctgtcgca gcacctggtg cagcgttaac ctggaatacc
    69061 acctgttttg cccgatatgg ctgtccatca ggaccggtca cgctatcagc atttgccaga
    69121 ttactggccg ccacgttcag gcgctgggac tgggcagtta acgccgaccc ggcgatatca
    69181 aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat catgcctttg
    69241 atttgcccgc tcaacgcgct aaggctcatc tggtattgca ggctgttatc ggcaaactgg
    69301 gtgacttcct gacgctgggc gcgcagattg agcgcctctt gttgaaaacg taaggcggcg
    69361 tcgagcttat cgagcatatc tcctccgcag gtatcaaaat tctgccatca gcttaaatgc
    69421 ctttacgcgc gcgttatcgg cggaataaac gcaaaatggg tcgctattta tgccgttgat
    69481 ggtcattgcg gacaggtaca actcacgttg tagaaatggc tgggggcgaa aatgctgaca
    69541 ataaaacgta gcgtggcgat catcgcgata ctgttcagtc cgttaagtac ggcgagcaat
    69601 ctcacatcgc aattgcacaa cttttttagc gcccaactcg cgggggtaag tgatgaggtt
    69661 cgtgtttcta ttcgtacagc gcccaatcta ctaccgccat gcgagcagcc attgctttcg
    69721 atgagcaata attcccgcct gtggggcaat gtgaatgtgt tggcacgctg cggtaacgac
    69781 aaacgatatt tacaggttaa tgtgcaggcc acaggaaatt atgtggttgc cgcgatgcct
    69841 attgtgcggg gaggaaagct ggaagctggc aatgtcaaac tgaaacgtgg acgcctggat
    69901 accctgccac cgcgtacggt gctggatatc aatcaacttg ttgatgccgt tagcctgcgc
    69961 gatctatcac ccgatcaacc tatccagtta acccagtttc gccaggcatg gcgggtaaaa
    70021 gcgggacaac gcgtcaatgt gatcgccagc ggtgatgggt ttagcgccaa cgcagaaggt
    70081 caggcgctga acaatgcagc cgtcgcacag aatgcgcggg tgcgcatggt atcgggacag
    70141 gtagtcagcg gcgttgttga tgcagatggg aatattctta taaacctgta agctgttaaa
    70201 gattacccgt cccttgccga taaataagca acacatgata aaagcgccct caatgaggaa
    70261 taaaccatga gtattgatcg cacttcgcct ctgaagcctg taagcaccgt tcaaccgcgc
    70321 gaaaccactg acgcgccggt aacgaacagc cgcctccacc agcaccagtg tgacgttaag
    70381 cgacgcgcaa gcaaaactga tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga
    70441 agcgttaaaa ctggcgattc gtaacggtga actaaaaatg gacaccggca aaattgccga
    70501 tgcgctgatc aacgaagcgc agcaagactt gcagagtaac tgatcgtatg acacgtcttg
    70561 cagagatcct cgaccagatg tccgctgtgc ttaacgatct caaaacggta atggatcatg
    70621 agcagcaaca tctctctatg gggcagatca acggcagcca gttgcaatgg attacagaac
    70681 aaaaaagctc actgctggcg acgctggatt acctcgaaca gttacgcaag aaagaaccca
    70741 atacagcaaa tagcgttgat attagtcaac gctggcagga aattactgtg aaaacgcagc
    70801 aactacgcca aatgaatcaa cataacggct ggttactgga aggacagatt gagcgcaatc
    70861 aacaggcgct ggaaatgttg aaaccgcatc aggaaccgac gctatatggg gcgaacggtc
    70921 agacctcaac accccatcgc ggcagtaaaa agatttcgat ctgaatatta tctccggcct
    70981 gcactgcagg ccggaatgca ttgttacacc gtccggcggg caaattcttt aactttgaag
    71041 cccagtaccg ccagtgcagc gaagtacgcg gcaatccccg ccagcacgac cgccattaaa
    71101 cgcagtaagc gccagggcat ggtacccaat gaccactccg gcatgatatg taacatacct
    71161 aaaagcacgc cagacatcac cagtaccgcc accaccagac gcaacagaaa cgccatccag
    71221 ccgggttgcg gagtaaagat tttctgctta cgcaactgcc agtaaagcag cgaagcattc
    71281 agacacgccg ccagaccaat agaaagtgac agcccggcat gtttcaacgg accaataaac
    71341 gccaggttca tcaattgcgt taaaatcagc gtaacgatgg caattttcac tggcgtttta
    71401 atgtcttggc gggaataaaa gccaggagcc aacactttca ctacaatcag gccaatcaaa
    71461 cccaccgagt aggcaattaa cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta
    71521 ccgtactgga acagcgaaac ggttaacgga cctgaaagaa tgcccaacgc aaccgcactc
    71581 ggcagtgcca acaggaaaca aagacgcaac ccccagtcca tcaaacggtt gtattcatca
    71641 tgattgccac tggcaaaact tttcgacagc gacggcagca aaatggtgcc aagcgccacg
    71701 cccagcacac cggagggaaa ctccattaag cggtcggcgt aatacatcca agacaccgaa
    71761 ccggaagcaa gaaacgaggc aaaaatggtg ttgatgatta aggagatctg gctcacagag
    71821 acgccaagga tcgccggtcc catctgtttc accacgcgca ttgctccagc atcgtggaag
    71881 ttaatgcgcg gcaggaccag catgccgatc ttcttcaggt gcggtagctg atacaccagc
    71941 tgcaggatgc cgccgaccgt aacagcccac gccagcgcca gcaccggtgg gttaaagtac
    72001 ggtgcggcaa acagcgcgaa accaatcatg ctgatgttaa gcagtgttgg cgcaaacgcc
    72061 ggaatcgaga agcggttcca cgtattcaga atcgctccca ccagcgacgc cagggagatc
    72121 agcaagatat agggaaaggt aatctttagt aggtggctgg tcagggcaaa tttgtcagct
    72181 gtgtcagcga agcctggcgc ggtcaccatg atcacccacg gtgcggcgag catgccagcg
    72241 accgtcacaa ccgccagcgc aagtgtcagc aggccagaaa cataagagac aaagacccgc
    72301 gtggcgtctt caccctgctt acttttatat tccgccagaa tcggtacaaa tgcctgggaa
    72361 aatgcccctt cggcaaagat acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag
    72421 gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca
    72481 agcacacgcg aaaacatggt catcgagctg acggcggcca gcgattttaa taaattcatc
    72541 ggtgttctaa tccggaccca cgggcaaaac gcctgcaagg caggcgctga aagaggcgaa
    72601 tagtctacct agattatggt gaattgctac cgccagatgt tacagggttt attcactcat
    72661 cgcatcgcgc cagatcttgt caacgatacg ttgcgccagc actgcctatt cgccggcggt
    72721 ttgcggaact gtctgatttt gcacgcattc aatgaagtgc cgcgcacagc cgacaaatcc
    72781 acgttgctca agagtgctct gccaaccagg aatcggttta ttcactacac cctgcccgcg
    72841 ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg
    72901 cacggtttca cgctgacttc cggcacggcg atgcatacaa gtggtgatct gcaaaggacc
    72961 ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg tcgttagtca gtagcgtacc
    73021 gccatccaga gaggctttgc cgcccgacaa ccacagcgcg gtatccacca catgcagata
    73081 atcatccagc aacgtgaaat aaagatcgtg tggcccgacg ctattgctac gatgtttatc
    73141 cattcttagc gaggctgcgg tggcgagttg cgtttttaac tcaccgtaga gtggtgcgaa
    73201 acgacggtta aaaccgacca tcaacgtcag ttttttccgc gccgccagtt ccaccagcct
    73261 ttcagcatcg cgcagatttt ctgccagcgg tttatcgaca cagacatgta cccccgcatt
    73321 gagtaacgta ctgaccacgt caaagtggct ggcggtgctg gagtgcacaa aaaccgcatc
    73381 acaactggcg gcaaggctgg ataacgaatc ggcataagga atgcgccagc tgtcacaaat
    73441 tggcagggct ttggcgcgcg taggcgacca ggctccttgt aacgtccagt cagacgctgc
    73501 cgccagcacc ggtaaccacg ctttttgcgc aatgccgcct aatcccacta cgccgatacg
    73561 taattttttc actttaatct cccagatggg ccagcaacga atcaagacgt tgtttcagtt
    73621 ctgccacttc gatttccagg gcttcgacgc gggtctgtaa atcaccgtca accatgttcg
    73681 acatagccgt taccgccggc tgatcttcaa cctcaccact gaaaagatgc atgtagcggc
    73741 tttcgcgttt acccggttcg cgggccagac gcaccacaaa aggaccatct tcgcgattcg
    73801 ccagttgttc cagcgtcgac tccacttccg ccatatcgct gaattcatac attcgcgcgg
    73861 cgcggctgcg cagttcaccc agcgtctggg caccacgcaa taacaacgtg gtgattaacg
    73921 ccacttctgc ggcgctcagt ttcagatcgc caaattctga attacaaaaa cgttgctcat
    73981 atttggtgac ccgattacca aaaccgctca ctgtgcgtag ataatgacgt ttgaccagat
    74041 tatccagctg ttcctgcact tcggattcac tcagattcat gaccggttca cggttcgttt
    74101 tctgattaca ggccgtgact acaccattga ctgagagcgg gtattgctcc ggcgtggtca
    74161 cctgcttttc cagcaagcaa ccaattacgc gagcttccag ggcagtaagt tgatatttca
    74221 tgagattctc cttagcggcc gggcgtccag tctggggtag ttaatgccgt cagtacgtga
    74281 tcgcgccatt gtccatcaat caacagatag tctttcgcat agccttcttt ttcaaaaccc
    74341 agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca tataattagc cataatgcga
    74401 tgaatatgtt gggtgcgctg catataacga atggctgcgg tcagggcttc aaacatgagt
    74461 cctttgccct gccatttttg cccaatcgaa taaccgagat agcaggcatg aaaagagccg
    74521 cgaacaacat tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg gtcgaataag
    74581 ccaaagtaga aagctgaacc ttgtttatga aattcgttaa tcatccctag cctggcctgc
    74641 cagcctgatg gataacagtg gctttcgtcg cgcactggct cccagggctt gaggaaatgg
    74701 cgattctctg cgtaataatc cgccagacgc caggcatcac gatcatgcac cagacgcacg
    74761 accagtcggt ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc aaacattcta
    74821 tacctactcc ttcccgtaac gtctttaata cgctgtaagt attactatac ctgcgcaacc
    74881 agaccctgtg aaaagtccaa aatgccattt tctgcattat tcacatattt gataaaaact
    74941 ctctatcaaa gcgacgtttt gataaaaaaa tattgtcccc gaagtgatgg gaaaagtctt
    75001 agcggcaatg cagaatagaa agtgctcatc ttttatttcc cgggagggga aatgtcccgc
    75061 gtatcgcagg cgaggaacct gggtaaatat ttcctgctca tcgataatat gctggtcgtg
    75121 ctggggttct ttgttgtctt cccgctgatc tctatccgct tcgttgatca aatgggctgg
    75181 gccgccgtca tggtcggtat tgctctcggt ctacgccagt ttattcagca aggtctgggt
    75241 attttcggcg gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt taccggtatg
    75301 ctgatgcgcg ccgccggatt cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg
    75361 ttttcatgcc tgctctcggg actcggcggc acgttgtttg atccgccgcg ttcggcgttg
    75421 gtggtgaaac taatccgtcc acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag
    75481 gacagtgcca gtgcggtcat tggcgcattg ttggggagct ggctgttgca atacgacttt
    75541 cgcctggtct gcgccacagg ggccgttttg tttgtgctat gtgcggcgtt caatgcgtgg
    75601 ttgttaccgg catggaaact ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc
    75661 gtgatgcgtg acaagcgttt tgtcacctat gtcctgacgc tggcgggtta ctacatgctg
    75721 gctgtacaag tgatgctgat gctgccaatt atggtcaacg acgtggctgg cgcgccctct
    75781 gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct ctaccctatc
    75841 gcccgctgga gtgaaaagca ttttcgtctg gaataccggt tgatggctgg gctgttgata
    75901 atgtcattaa gcatgatgcc ggtgggcatg gtcagcggcc tacaacaact tttcaccctg
    75961 atttgtctgt tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct
    76021 tcgctggcgg acgcaagagc tcgcggcagc tatatggggt ttagccgtct gggtctggcg
    76081 attggcggcg ctattggtta tatcggtggt ggctggctgt ttgacctggg caaatcgacg
    76141 caccagccag agcttccgtg gatgatgctg ggaattattg gcatcttcac tttccttgcg
    76201 ctgggttggc agtttagtca gaaacgcgcc gcgcgtcgtt tgctggaacg cgacgcctga
    76261 tttgatacac ccccctgact cctccatact gaaagtgaac tgacagccaa atggaggagt
    76321 cacgtgaagc tatacattta cgatcactgc ccttactgcc tcaaagcccg catgattttc
    76381 ggcctgaaaa atatccccgt cgaattacat gttctgctca acgacgacgc agaaacaccc
    76441 acccggatgg tcggtcaaaa acaggttccc attctgcaaa aagatgacag ccgctatatg
    76501 ccagaaagca tggatatcgt tcactatgtc gataaactcg acggcaaacc gttactgacc
    76561 ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg tcaatggcta cgccaacaaa
    76621 ctgctgttgc cgcgttttgc caaatcggca tttgatgagt tttctactcc cgccgcgcgc
    76681 aaatatttcg tcgacaagaa agaggccagc gcgggtaatt ttgccgacct gctggcccac
    76741 tctgacggtc tgattaagaa tatcagcgat gatttgcgtg cgctggacaa actgatcgtc
    76801 aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata ttcagctatt tccgctactg
    76861 cgtaatctga cgctggtagc cggaatcaac tggccaagcc gcgttgctga ttaccacgat
    76921 aatatggcga aacagacaca aatcaatttg ttatcatcaa tggcgattta atcctgaccc
    76981 gccgcggcag cctgtattcg cgggttgccg ccgttttcag ttttttctgc tggcgagttt
    77041 atcgctggtg acacatgctg cgttgtgtca ggatattgac gcagatttca tgtcgaggac
    77101 taccatgaac aagtttttat ttgccgctgc attgatcgtc agtggcctgc tcgttggctg
    77161 taatcaactc acccaataca ccatcaccga acaagaaatt aaccagtcgc ttgcgaaaca
    77221 taataatttc tcaaaagata tcggtttacc cggcgtggct gacgcccata ttgttctgac
    77281 aaacttgacc agccaaattg gtcgcgaaga gccgaataag gttaccctaa ccggagacgc
    77341 caatctggac atgaactccc tgttcggtag tcagaaagcg accatgaaac tgaagctgaa
    77401 agcgctgccc gtgtttgata aagaaaaagg tgcgatcttc ctgaaagaga tggaagtggt
    77461 cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg caaacgttgc ttccctattt
    77521 gaaccaggca ttacgcaatt actttaacca gcaacctgct tacgtcctgc gcgaagatgg
    77581 cagtcagggc gaagcaatgg cgaaaaaact ggcgaaaggc attgaagtga agccaggtga
    77641 aattgtcatt ccgtttacgg attaatgacg agggcgcatt cgcgcccttt atttttcgtg
    77701 caaaggaaaa catttccgct tatcctttgt gtccggcaaa aacatccctt cagccggagc
    77761 atagagatta atgactgcac catcccaggt attaaagatc cgccgtccag acgactggca
    77821 ccttcacctc cgcgatggcg acatgttaca aactgtcgtg ccgtatacca gcgaaattta
    77881 tggacgggct atcgtaatgc ccaatctggc tccgcccgtg accacagttg aggctgccgt
    77941 ggcgtatcgc caacgtattc ttgacgccgt acctgccggg cacgatttca ccccattgat
    78001 gacctgttat ttaacagatt cgctggatcc taatgagctg gagcgcggat ttaatgaagg
    78061 cgtgttcacc gctgcaaaac tttacccggc aaacgcaacc actaactcca gccacggcgt
    78121 gacgtcaatt gacgcaatca tgccggtact tgagcgcatg gaaaaaatcg gtatgccgct
    78181 actggtgcat ggtgaagtga cacatgcaga tatcgacatt tttgatcgtg aagcgcgctt
    78241 tatagaaagc gtgatggaac ctctgcgcca gcgcctgact gcgctgaaag tcgtttttga
    78301 gcacatcacc accaaagatg ctgccgacta tgtccgtgac ggaaatgaac ggctggctgc
    78361 caccatcact ccgcagcatc tgatgtttaa ccgcaaccat atgctggttg gtggcgtgcg
    78421 tccgcacctg tattgtttac ccatcctcaa acgcaatatt caccaacagg cattgcgtga
    78481 actggtcgcc agcggtttta atcgagtatt cctcggtacg gattctgcgc cacatgcacg
    78541 tcatcgcaaa gagagcagtt gcggctgcgc gggctgcttc aacgccccaa ccgcgctggg
    78601 cagttacgct accgtctttg aagagatgaa tgctctgcag cactttgaag cattctgttc
    78661 tgtaaacggc ccgcagttct atggcttgcc ggtcaacgac acattcatcg aactggtacg
    78721 tgaagagcaa caggttgctg aaagcatcgc actgactgat gacaccctgg tgccattcct
    78781 cgccggggaa acggtacgct ggtccgttaa acaataaaaa tcgtagcttc ctgttgtcat
    78841 taggttattt tacctgtata aataaccagt atattcaaca gggggctatt atgcgaattg
    78901 aagtcaccat agcgaaaact tctccattgc cagctggggc tattgacgcc ctggctggcg
    78961 aactttcccg ccgtattcag tatgcgtttc ctgataatga aggccacgta tcggtacgtt
    79021 atgccgcagc gaataattta tcggttattg gcgcaacaaa agaagataaa cagcgcatta
    79081 gcgaaattct ccaggaaacg tgggaaagcg ccgatgactg gtttgtcagc gaataatatg
    79141 cagtgatttt tgtttttgcc gggtcgcccc ggctttttta ttttacttac gaattttgag
    79201 tacaaaattt ctacacttcc tcaaaataat tgcacaatat gctaaaaaaa tatgaatgac
    79261 atgctgtttt attgttcata taaatcttaa aagcagaaat ttatctgttc ccccctttaa
    79321 aaattcggtg aataccctta cttattggta tactgaagtt gcttcagata aaaacgcatt
    79381 gaacctcgaa taacgttgtc tagtaacacg aattaggggg ccatgatgga aaaaaataat
    79441 gaagtcattc agactcatcc gctcgtaggg tgggacatca gcaccgttga tagctatgat
    79501 gcgctgatgt tgcgtttgca ctaccagacc ccaaataagt ccgagcagga agggactgaa
    79561 gttggtcaga cgctctggtt aaccactgat gttgccagac aatttatttc gatattagaa
    79621 gcaggaatcg ccaaaattga atccggtgat ttcccggtaa acgagtatcg gcgtcattaa
    79681 catgacatga ccatccaccc aaaaggcacc tcaccggtgc ctttaccatt tctgggttgt
    79741 atatctgcat tcagttaatt actctgctta aaccatgaga gcagagagaa aaaaatgaaa
    79801 tacgatctca tcattattgg cagcggttcc gtaggcgctg ccgccgggta ttatgcaacc
    79861 cgcgccggtt taaacgtgct aatgaccgac gcccatatgc caccgcatca acacggcagc
    79921 caccacggcg atacgcgatt aattcgccat gcttatggtg aaggcgaaaa gtatgtcccg
    79981 ttagtcctcc gcgcgcaaac gctgtgggat gaactttccc gccacaacga agatgatccc
    80041 atttttgtac gctctggtgt cattaacctc ggcccggctg actccgcatt tctcgccaac
    80101 gtcgcccaca gcgccgaaca gtggcaactc aacgttgaaa agctcgatgc gcaagggatt
    80161 atggcccgct ggccagaaat acgcgtcccg gacaactaca tcggcttatt tgagactgat
    80221 tccggttttt tgcgcagcga actggcgatt aaaacctgga tccaactggc gaaggaagcg
    80281 ggctgtgcgc aactgttcaa ctgcccggtc accgcaattc gtcatgacga tgatggcgta
    80341 actattgaaa cggttgacgg tgagtatcag gcgaaaaaag cgattgtctg cgcgggaaca
    80401 tgggtaaaag acctgctccc ggagctgcct gtccagcccg tacgtaaagt atttgcctgg
    80461 tatcaggccg atggccgcta tagcgtgaag aataaattcc cggcgtttac cggtgaactg
    80521 cccaatggcg atcaatatta tggttttccg gcagaaaacg acgcgttgaa gattggcaaa
    80581 cataacggag gccaggttat ccattcagcg gatgaacgtg ttccgtttgc ggaagtggcc
    80641 agtgatgggt cagaagcctt cccgttcttg cgcaatgtat tgccgggtat cggttgctgc
    80701 ctgtacggcg ctgcctgcac ctatgataat tcgcctgacg aagattttat tatcgatacc
    80761 ctacccggcc acgataatac actgctcatt accggcctga gtgggcacgg ttttaaattt
    80821 gcgtcagttt taggggaaat agctgccgat tttgcgcaag accaaaaaag cgattttgat
    80881 ttgacgccat tcaggctttc ccgcttccaa taatcatcat tacggcctcc agaatgaggc
    80941 cgtttttaca ggagtattta atgcgtcggc tgttgcacta tctcatcaat aatattcgcg
    81001 agcatctgat gctttatctt tttctctggg gattgttggc aattatggat ctgatctatg
    81061 tgttttattt ttagttttcc gcactgacat aaactgacaa atatttaatc tccctttcat
    81121 ttagcgtgaa aaattgaatg agattattcc tgcttctaca gcactcacca gacatctctg
    81181 atgctgaacc cactaaactt atgaagattc tttcattatt aattcaaaat tattgaaata
    81241 aaaataaata tcattcaatt tgttattttt gtgttgcaat tcattctccg acacgccatt
    81301 ttctttctct cgcaaattcg tattggatgc acttatgtca ttcacaaata cccctgaacg
    81361 ctatggtgtt atatctgcgg ccttccattg gctaagcgca attattgtct acggcatgtt
    81421 tgctttaggt ctgtggatgg tcacgctcag ttattacgat ggctggtatc acaaagcacc
    81481 cgaactgcat aaaagcatcg gtattttgtt aatgatgggg ctggttattc gcgttctgtg
    81541 gcgtgtcata tctccaccgc cgggtccgct gccgagttat tcgcccatga ctcgtcttgc
    81601 cgcaaaagct ggacatctcg cattgtacct attgctgttc gccatcggca tcagcggcta
    81661 tctgatctca actgccgatg gtaaaccgat cagcgttttt ggctggtttg acgtccccgc
    81721 gacccttgcc gacgctggcg cacaggcaga ctttgccggt gccctgcatt tttggctcgc
    81781 gtggagcgtc gtagtactgt ctgttatgca cggatttatg gccctgaagc atcatttcat
    81841 cgataaagac gacactctga agcgcatgct gggaaagtcg tcatctgact atggagtata
    81901 aaaaatgaaa aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc
    81961 agcggttgcc gccgattaca aaattgacaa agaaggtcag cacgcctttg ttaacttccg
    82021 catacagcat cttggctata gctggttata tggcaccttt aaagatttcg acggtacttt
    82081 tacctttgat gaaaaaaatc cggctgccga taaagtgaat gtgacaatta acaccaccag
    82141 cgtcgatact aaccacgccg aacgcgataa acatcttcgc agtgcggatt ttctcaatac
    82201 cacaaaatat ccacaggcaa cattcacctc caccagcgtg aagaaagacg gtgacgaact
    82261 ggatattacc ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc tggaagcgaa
    82321 attaattggt cagggcgacg acccatgggg cggtaaacgt gcaggctttg aggccgaagg
    82381 caaaattaag ctcaaagact tcaatatcaa gacagattta ggtccagctt ctcaggaagt
    82441 ggatctgatt atttcagtgg aaggcgtaca gcagaagtaa gagtgaaatg ttgccggatg
    82501 gcacgcatcc ggcaatgata tttattctgt tggatcagga atacccagtg ttgtattcag
    82561 acgtccacga gacttattaa agatcttatt gccattttca cgtcctgccc gacggcgtcg
    82621 ctgttcttct ggcggtaacg cgctttcttc gcagcaaatc tcactacaac aacctttgta
    82681 tttttccgcg catactggac actgaataaa cagcaggtgg cagccatcat ttttacagtt
    82741 ggtatggctg tcgcacggcg cgccgcactg gtggcaatgc gcgataatct catccgatat
    82801 acgttcgccc atccgctcgt caaaaacaaa atttttgcca ataaaacgca ccggcaagcc
    82861 ctgctcgcgc gccttacggg cgtattcaat aattccgccc tcgatatgcc agactttgtt
    82921 gaatccgtta tgtttcatcc aggcactggc cttttcacaa cgaatgccgc cggtgcagta
    82981 catgacgatt tttttatctt tatgtgcctg catcatctcg actgcttttg gcagctgctc
    83041 acggaaggta tctgccggaa tttcgagcgc gttttcaaag tgccccactt catactcata
    83101 gtggttacgc atgtcgataa atagtgcatc gggatcgtca agcatggcgt tcacttccgc
    83161 cgcttgcaga tactcaccaa cattgctggc atcaaagtga ggatcgtcaa taccgtcggc
    83221 aacaatgcga tcgcgtacct tcatgcgcag tacccagaag gatttcccgt catcatccaa
    83281 cgcgatattc aggcgtaaac cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa
    83341 tgtttcaaca ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat gcgccagata
    83401 cactcgccca aaaacattca gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt
    83461 aggatcggcg atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt cgctctcagc
    83521 caacattttg gcttttaacg cgtcgttgga aatgcggttg tgtaacactg gcatggtgta
    83581 cggttcctgc gagatgggaa agtaaaaatc cgcggcatga tatagcaatt atcgataatt
    83641 aacatccaca cattttacgc tacatttgcg cattaaaaat tatttgttat ttacaagcgc
    83701 ggcaatttcg cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata
    83761 catacaattg cccgtatagg ttgaaaaaca ggattgatat gacgaatcta cccaagttct
    83821 ccaccgcact gcttcatccg cgttattggt taacctggtt gggtattggc gtactttggt
    83881 tggtcgtgca attgccctac ccgattatct accgcctcgg ttgtggatta ggaaaactgg
    83941 cgttacgttt tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc
    84001 cggaaatgag cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca
    84061 tgggcctgat ggaaaccggt atggcgtggt tctggccgga ccgccgaatc gcccgctgga
    84121 cggaagtgat cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt
    84181 tagttggcat ccattttctg acactggagc tgggcgcgcg gcagtttggt atgcaggaac
    84241 cggggattgg cgtttaccgc ccgaacgata atccactgat tgactggcta caaacctggg
    84301 gccgtttgcg ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc atgattaaag
    84361 ccctgaaaaa aggcgaagtg gtctggtacg caccagatca tgattacggc ccgcgctcaa
    84421 gcgttttcgt cccgttattt gccgttgagc aggctgcgac cacgaccgga acctggatgc
    84481 tggcacggat gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag ccagatggca
    84541 aagggtatca attgattatg ctgccgccag agtgttctcc gccactggat gatgccgaaa
    84601 ctaccgccgc gtggatgaac aaagtggtcg aaaaatgcat catgatggca ccagagcagt
    84661 atatgtggtt acaccgtcgc tttaaaacac gcccggaagg cgttccttca cgctattaaa
    84721 tctcccatgc cggatgcttc agaatggcat ccggcattac cacagcaaat ccccctgatt
    84781 tagcgataaa agctctctgg attgcgcccc ctggaagtcg ggcgcataat tagtgtgttt
    84841 atcttttctt cttatgttca ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct
    84901 gtgaaaatga cacccctata aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt
    84961 ttcttaccgg ggccgccttc agtctggtaa tgcccttctt acccctctac gttgagcagc
    85021 ttggtgttac cggccactcc gccctgaata tgtggtccgg tattgtcttc agtataatgg
    85081 gttcctaaac acatcatttt agatattgaa tatgagacgg gcaatcagct acatcagatt
    85141 ttcaagtgaa cgacaactga aaggggactc tgtacgccga caatctaagc tggttactga
    85201 ctggcttgat aagaaccctg agttttactt ggattcatcc ttgagcttta aagatttggg
    85261 gaaaagtgct tttagtggta agcacctgaa aggtggtcta ggtgactttt taaccgctat
    85321 cgagaaaggg cttgttaaag ctggtgatac gctactaatc gagagtttag accgtctgtc
    85381 acgtcaggat attgatattg cttctgagct attacgccgt atcttaaggg ctggtgtaga
    85441 tgtagtcaca ctgagcgatg gagaacatta cacacgagaa agtttaaaag acccgttagc
    85501 cctgattaaa agcattctga taatgcagcg ggcgcatgag gaaagtttga ggaagtctga
    85561 gcgtgtccaa gctgcatgga accgtaaaaa agaactcatc tccgaaggta tcaaggtatc
    85621 taggcgttgt cctgcatggt taaggctgaa tgatgaccgt cgaacattca caatcattcc
    85681 tgataaagtg gaagttgtta aacgagcatt tgacctgaga ttacagggtt taagcttttg
    85741 ggctatcacc agaactttaa acgatgaagg tcacttgtca ttgaatcaat acacacccaa
    85801 acaaaagggg tggtctgata ctgctgtaaa gaaactactc agaaatcgtg ctgtgattgg
    85861 atgtttcaca cctgctggac gtgaagaagt tcagggttac taccccgcaa taatcagtga
    85921 gtcactattc tatcgagtac agcaactgaa cacagggcaa tatggtaggg cttctgtatc
    85981 aagtaatccg ctatcagtca acttgttcag gggtatcatc aaatgttctg agtgtggggc
    86041 tactattgtt cttggtggat atgccttaaa acgttttgga atgtacagat gccctaatcg
    86101 ttcagctaac aggtgtagtg caaaggctat cagtcgtaag caaacggatg caactatcct
    86161 ttacatgctc gcattatgcg accgttttga aactgaaaat actgacacta ttgatagcct
    86221 taaattacag cgtgaagatt tg
//
LOCUS       DAJTXY010000004        82505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTXY010000004 DAJTXY010000000
VERSION     DAJTXY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.004"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..368)
                     /locus_tag="OO566_000338"
     CDS             complement(<1..368)
                     /locus_tag="OO566_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR6603753.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLG"
     gene            complement(439..2553)
                     /gene="fusA"
                     /locus_tag="OO566_000339"
     CDS             complement(439..2553)
                     /gene="fusA"
                     /locus_tag="OO566_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6603754.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2650..3120)
                     /gene="rpsG"
                     /locus_tag="OO566_000340"
     CDS             complement(2650..3120)
                     /gene="rpsG"
                     /locus_tag="OO566_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6603755.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3217..3591)
                     /gene="rpsL"
                     /locus_tag="OO566_000341"
     CDS             complement(3217..3591)
                     /gene="rpsL"
                     /locus_tag="OO566_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6603756.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3717..4004)
                     /gene="tusB"
                     /locus_tag="OO566_000342"
     CDS             complement(3717..4004)
                     /gene="tusB"
                     /locus_tag="OO566_000342"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6603757.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4012..4371)
                     /gene="tusC"
                     /locus_tag="OO566_000343"
     CDS             complement(4012..4371)
                     /gene="tusC"
                     /locus_tag="OO566_000343"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6603758.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4371..4757)
                     /gene="tusD"
                     /locus_tag="OO566_000344"
     CDS             complement(4371..4757)
                     /gene="tusD"
                     /locus_tag="OO566_000344"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6603759.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4757..5479)
                     /locus_tag="OO566_000345"
     CDS             complement(4757..5479)
                     /locus_tag="OO566_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6603760.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5646..6458)
                     /gene="fkpA"
                     /locus_tag="OO566_000346"
     CDS             complement(5646..6458)
                     /gene="fkpA"
                     /locus_tag="OO566_000346"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6603761.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6679..6897
                     /locus_tag="OO566_000347"
     CDS             6679..6897
                     /locus_tag="OO566_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6603762.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6946..7536)
                     /gene="slyD"
                     /locus_tag="OO566_000348"
     CDS             complement(6946..7536)
                     /gene="slyD"
                     /locus_tag="OO566_000348"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6603763.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETEQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7631..7831)
                     /locus_tag="OO566_000349"
     CDS             complement(7631..7831)
                     /locus_tag="OO566_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6603764.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7841..9646)
                     /gene="kefB"
                     /locus_tag="OO566_000350"
     CDS             complement(7841..9646)
                     /gene="kefB"
                     /locus_tag="OO566_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR6603765.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9646..10197)
                     /gene="kefG"
                     /locus_tag="OO566_000351"
     CDS             complement(9646..10197)
                     /gene="kefG"
                     /locus_tag="OO566_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6603766.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10328..12241
                     /locus_tag="OO566_000352"
     CDS             10328..12241
                     /locus_tag="OO566_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6603767.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12241..13263
                     /locus_tag="OO566_000353"
     CDS             12241..13263
                     /locus_tag="OO566_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6603768.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13257..13475
                     /locus_tag="OO566_000354"
     CDS             13257..13475
                     /locus_tag="OO566_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6603769.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13529..14398
                     /locus_tag="OO566_000355"
     CDS             13529..14398
                     /locus_tag="OO566_000355"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6603770.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14453..14857)
                     /locus_tag="OO566_000356"
     CDS             complement(14453..14857)
                     /locus_tag="OO566_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6603771.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15159..15791
                     /gene="crp"
                     /locus_tag="OO566_000357"
     CDS             15159..15791
                     /gene="crp"
                     /locus_tag="OO566_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6603772.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15842..17932
                     /locus_tag="OO566_000358"
     CDS             15842..17932
                     /locus_tag="OO566_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6603773.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17999..19219)
                     /gene="argD/dapC"
                     /locus_tag="OO566_000359"
     CDS             complement(17999..19219)
                     /gene="argD/dapC"
                     /locus_tag="OO566_000359"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6603774.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19305..19868)
                     /gene="pabA"
                     /locus_tag="OO566_000360"
     CDS             complement(19305..19868)
                     /gene="pabA"
                     /locus_tag="OO566_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6603775.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19900..20502)
                     /locus_tag="OO566_000361"
     CDS             complement(19900..20502)
                     /locus_tag="OO566_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6603776.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20492..20659)
                     /locus_tag="OO566_000362"
     CDS             complement(20492..20659)
                     /locus_tag="OO566_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6603777.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20764..21336)
                     /gene="ppiA"
                     /locus_tag="OO566_000363"
                     /gene_synonym="rotA"
     CDS             complement(20764..21336)
                     /gene="ppiA"
                     /locus_tag="OO566_000363"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6603778.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21607..22788
                     /gene="tsgA"
                     /locus_tag="OO566_000364"
     CDS             21607..22788
                     /gene="tsgA"
                     /locus_tag="OO566_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6603779.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23050..25593
                     /gene="nirB"
                     /locus_tag="OO566_000365"
     CDS             23050..25593
                     /gene="nirB"
                     /locus_tag="OO566_000365"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6603780.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKGDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25590..25916
                     /gene="nirD"
                     /locus_tag="OO566_000366"
     CDS             25590..25916
                     /gene="nirD"
                     /locus_tag="OO566_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6603781.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26042..26848
                     /gene="nirC"
                     /locus_tag="OO566_000367"
     CDS             26042..26848
                     /gene="nirC"
                     /locus_tag="OO566_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6603782.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26867..28240
                     /gene="cobA"
                     /locus_tag="OO566_000368"
     CDS             26867..28240
                     /gene="cobA"
                     /locus_tag="OO566_000368"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6603783.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28487..28654
                     /locus_tag="OO566_000369"
     CDS             28487..28654
                     /locus_tag="OO566_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6603784.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28949..30286
                     /gene="frlA"
                     /locus_tag="OO566_000370"
                     /pseudo
     CDS             28949..30286
                     /gene="frlA"
                     /locus_tag="OO566_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30307..31329
                     /gene="frlB"
                     /locus_tag="OO566_000371"
     CDS             30307..31329
                     /gene="frlB"
                     /locus_tag="OO566_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6603785.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITLLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31379..32209
                     /gene="frlC"
                     /locus_tag="OO566_000372"
     CDS             31379..32209
                     /gene="frlC"
                     /locus_tag="OO566_000372"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6603786.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIYMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32206..32991
                     /gene="frlD"
                     /locus_tag="OO566_000373"
     CDS             32206..32991
                     /gene="frlD"
                     /locus_tag="OO566_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6603787.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33091..33822
                     /gene="phoB"
                     /locus_tag="OO566_000374"
     CDS             33091..33822
                     /gene="phoB"
                     /locus_tag="OO566_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6603788.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33982..35067)
                     /locus_tag="OO566_000375"
     CDS             complement(33982..35067)
                     /locus_tag="OO566_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6603789.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35079..36383)
                     /locus_tag="OO566_000376"
     CDS             complement(35079..36383)
                     /locus_tag="OO566_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6603790.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEMALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36395..36686)
                     /locus_tag="OO566_000377"
                     /pseudo
     CDS             complement(36395..36686)
                     /locus_tag="OO566_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 family protein"
     gene            complement(36697..37575)
                     /locus_tag="OO566_000378"
     CDS             complement(36697..37575)
                     /locus_tag="OO566_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6603791.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37572..38798)
                     /locus_tag="OO566_000379"
     CDS             complement(37572..38798)
                     /locus_tag="OO566_000379"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6603792.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38799..39962)
                     /locus_tag="OO566_000380"
     CDS             complement(38799..39962)
                     /locus_tag="OO566_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6603793.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(40046..40408)
                     /locus_tag="OO566_000381"
     CDS             complement(40046..40408)
                     /locus_tag="OO566_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6603794.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40425..41330)
                     /locus_tag="OO566_000382"
     CDS             complement(40425..41330)
                     /locus_tag="OO566_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603795.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41600..42604)
                     /gene="trpS"
                     /locus_tag="OO566_000383"
     CDS             complement(41600..42604)
                     /gene="trpS"
                     /locus_tag="OO566_000383"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6603796.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42597..43355)
                     /gene="gph"
                     /locus_tag="OO566_000384"
     CDS             complement(42597..43355)
                     /gene="gph"
                     /locus_tag="OO566_000384"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6603797.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43348..44025)
                     /gene="rpe"
                     /locus_tag="OO566_000385"
     CDS             complement(43348..44025)
                     /gene="rpe"
                     /locus_tag="OO566_000385"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6603798.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44043..44879)
                     /gene="dam"
                     /locus_tag="OO566_000386"
     CDS             complement(44043..44879)
                     /gene="dam"
                     /locus_tag="OO566_000386"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6603799.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44986..46272)
                     /gene="damX"
                     /locus_tag="OO566_000387"
     CDS             complement(44986..46272)
                     /gene="damX"
                     /locus_tag="OO566_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6603800.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46364..47452)
                     /gene="aroB"
                     /locus_tag="OO566_000388"
     CDS             complement(46364..47452)
                     /gene="aroB"
                     /locus_tag="OO566_000388"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6603801.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47509..48030)
                     /gene="aroK"
                     /locus_tag="OO566_000389"
     CDS             complement(47509..48030)
                     /gene="aroK"
                     /locus_tag="OO566_000389"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6603802.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48431..49669)
                     /gene="hofQ"
                     /locus_tag="OO566_000390"
     CDS             complement(48431..49669)
                     /gene="hofQ"
                     /locus_tag="OO566_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6603803.1"
                     /translation="MKQWIAALLLMLISGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49581..49985)
                     /gene="hofP"
                     /locus_tag="OO566_000391"
     CDS             complement(49581..49985)
                     /gene="hofP"
                     /locus_tag="OO566_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6603804.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADIRRTGGKAAKSDADGG"
     gene            complement(49975..50415)
                     /gene="hofO"
                     /locus_tag="OO566_000392"
     CDS             complement(49975..50415)
                     /gene="hofO"
                     /locus_tag="OO566_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6603805.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50399..50938)
                     /gene="hofN"
                     /locus_tag="OO566_000393"
     CDS             complement(50399..50938)
                     /gene="hofN"
                     /locus_tag="OO566_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6603806.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50938..51717)
                     /gene="hofM"
                     /locus_tag="OO566_000394"
     CDS             complement(50938..51717)
                     /gene="hofM"
                     /locus_tag="OO566_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6603807.1"
                     /translation="MTFKIWQIGLHLQQQEVAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51837..54389
                     /gene="mrcA"
                     /locus_tag="OO566_000395"
     CDS             51837..54389
                     /gene="mrcA"
                     /locus_tag="OO566_000395"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6603808.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54554..55114)
                     /gene="nudE"
                     /locus_tag="OO566_000396"
     CDS             complement(54554..55114)
                     /gene="nudE"
                     /locus_tag="OO566_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6603809.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55434..57569
                     /gene="igaA"
                     /locus_tag="OO566_000397"
     CDS             55434..57569
                     /gene="igaA"
                     /locus_tag="OO566_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6603810.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57634..58302
                     /gene="yrfG"
                     /locus_tag="OO566_000398"
     CDS             57634..58302
                     /gene="yrfG"
                     /locus_tag="OO566_000398"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6603811.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58313..58714
                     /gene="hslR"
                     /locus_tag="OO566_000399"
     CDS             58313..58714
                     /gene="hslR"
                     /locus_tag="OO566_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6603812.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58739..59617
                     /gene="hslO"
                     /locus_tag="OO566_000400"
     CDS             58739..59617
                     /gene="hslO"
                     /locus_tag="OO566_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6603813.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59752..61476)
                     /locus_tag="OO566_000401"
     CDS             complement(59752..61476)
                     /locus_tag="OO566_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6603814.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61855..63477
                     /gene="pckA"
                     /locus_tag="OO566_000402"
     CDS             61855..63477
                     /gene="pckA"
                     /locus_tag="OO566_000402"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6603815.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63553..64905)
                     /gene="envZ"
                     /locus_tag="OO566_000403"
     CDS             complement(63553..64905)
                     /gene="envZ"
                     /locus_tag="OO566_000403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6603816.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64902..65621)
                     /gene="ompR"
                     /locus_tag="OO566_000404"
     CDS             complement(64902..65621)
                     /gene="ompR"
                     /locus_tag="OO566_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6603817.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65849..66325
                     /gene="greB"
                     /locus_tag="OO566_000405"
     CDS             65849..66325
                     /gene="greB"
                     /locus_tag="OO566_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6603818.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66422..68743
                     /locus_tag="OO566_000406"
     CDS             66422..68743
                     /locus_tag="OO566_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6603819.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69181..69408
                     /gene="feoA"
                     /locus_tag="OO566_000407"
     CDS             69181..69408
                     /gene="feoA"
                     /locus_tag="OO566_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6603820.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69425..71746
                     /gene="feoB"
                     /locus_tag="OO566_000408"
     CDS             69425..71746
                     /gene="feoB"
                     /locus_tag="OO566_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6603821.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGMGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71746..71982
                     /gene="feoC"
                     /locus_tag="OO566_000409"
     CDS             71746..71982
                     /gene="feoC"
                     /locus_tag="OO566_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6603822.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72185..73063
                     /gene="rpnA"
                     /locus_tag="OO566_000410"
     CDS             72185..73063
                     /gene="rpnA"
                     /locus_tag="OO566_000410"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6603823.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            73136..73405
                     /locus_tag="OO566_000411"
     CDS             73136..73405
                     /locus_tag="OO566_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6603824.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73399..73764
                     /locus_tag="OO566_000412"
     CDS             73399..73764
                     /locus_tag="OO566_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6603825.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73765..74553)
                     /gene="bioH"
                     /locus_tag="OO566_000413"
     CDS             complement(73765..74553)
                     /gene="bioH"
                     /locus_tag="OO566_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6603826.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74591..75274
                     /gene="gntX"
                     /locus_tag="OO566_000414"
     CDS             74591..75274
                     /gene="gntX"
                     /locus_tag="OO566_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6603827.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75333..75908
                     /gene="nfuA"
                     /locus_tag="OO566_000415"
     CDS             75333..75908
                     /gene="nfuA"
                     /locus_tag="OO566_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6603828.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76269..77573
                     /gene="gntT"
                     /locus_tag="OO566_000416"
     CDS             76269..77573
                     /gene="gntT"
                     /locus_tag="OO566_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6603829.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77618..79702)
                     /gene="malQ"
                     /locus_tag="OO566_000417"
     CDS             complement(77618..79702)
                     /gene="malQ"
                     /locus_tag="OO566_000417"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6603830.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTTTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79712..82105)
                     /gene="malP"
                     /locus_tag="OO566_000418"
     CDS             complement(79712..82105)
                     /gene="malP"
                     /locus_tag="OO566_000418"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6603831.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19784 a        22408 c        20821 g        19492 t
ORIGIN      
        1 cccagcagga tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg
       61 atcgcgccgt ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc
      121 gggcagtcta cgtgtgcgta gtgacgggtc ggggtgtcat attcaacgtg agaagtgttg
      181 atggtgatac cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcacgagca
      241 gcaccgccgt aggttttagc cagtacggtg gtgattgcag cggtcagagt agttttaccg
      301 tggtcaacgt ggccgatagt accaacgtta acgtgcggtt ttgtacgttc aaatttttct
      361 ttagacacgg ctatattcct tactatagtg ctctcccctt caggagagag cacgggactt
      421 tggtattaac ccttaggctt atttaccacg ggcttcaatt acggcctgag caacgttact
      481 cggcgcttca tcatacttca ggaattccat agtgtatgat gcacgacctt tggtcagaga
      541 acgcagctga gttgcgtatc cgaacatttc agacagcggt acttcagcgt ggatcttaac
      601 gccagtaact tcagattcct gacctttgag cataccacga cgacggctca agtcaccgat
      661 aacgtcaccg gtgttctctt ccggagtttc tacttcaacc ttcatgatcg gctcaagcag
      721 aactggtttc gctttcttaa agccttcttt aaaggcgata gaagcagcca gtttaaacgc
      781 cagttcagag gagtcaacgt catggtaaga accgaagtgc agacgaatac ccatgtctac
      841 taccgggtag cctgccagcg gacctgcttt cagctgttcc tggatacctt tatcaacggc
      901 cgggatgtat tcgccaggga ttacaccacc tttaatgtcg ttgatgaact cgtagccttt
      961 cgggtttgaa cccggctcca gcgggtacat gtcgataaca acatgaccat actgaccacg
     1021 accaccagac tgtttcgcgt gtttaccttc aacatcggta actttctggc ggatagtttc
     1081 acggtaagca acctgcggtt tacctacgtt cgcttcaacg ttgaattcac gcttcatacg
     1141 gtcaacgatg atgtcgaggt gcagttcgcc catacccgcg atgatggtct ggttagattc
     1201 ttcgtcagtc catacacgga aagacgggtc ttctttagcc agacggccca gagccagacc
     1261 cattttttcc tggtcagctt tggttttcgg ttcaactgcg atggagatta ccggctcagg
     1321 gaattccata cgttccagaa tgatcggcgc atccgggtca cacagggtgt caccagtggt
     1381 tacgtctttc agaccgatag cagcagcgat gtcgcccgcg cgaacttctt tgatctcttc
     1441 acgtttgtta gcgtgcatct gaacgatacg accgaaacgc tcacgtgcag ctttcacgga
     1501 gttcagtacg gtatcaccag agttaaccac accggagtaa acacggaaga aggtcaggtt
     1561 accaacaaac gggtcggtag cgattttgaa cgccagtgca gagaacggct cgtcatcact
     1621 tgcgtgacgt tcagccggag tgtctttacc gtcgtccagg ataccgttga tcgcaggtac
     1681 gtcaaccggg gatggcaggt aatcaattac cgcatccagc atcgcctgaa cacctttgtt
     1741 cttgaacgca gaaccacagg ttaccaggat gatttcgttg ttcagaacgc gctgacgcag
     1801 agcacctttg atttctgctt cagtcagttc ttcaccaccc aggtattttt ccatcagctc
     1861 ttcagaagct tcagctgcgg attcgatcag gttctggtgc cattcgttag ccagttcaac
     1921 catgtctgcc gggatatctt cgtattcgaa ggttacgccc tggtcagcgt cgttccagtt
     1981 gatagctttc attttcacca ggtcaacaac accggtgaaa tgttcttcag caccaatcgc
     2041 cagctgcagc ggaaccgggt tcgcgcccag acgggttttg atctggttaa caactttcag
     2101 gaagttcgca cccatgcggt ccattttgtt aacgaacgca atgcgcggaa ctttatattt
     2161 gtttgcctga cgccatacgg tttcagactg cggctgaaca ccaccaactg cgcagtaaac
     2221 cattaccgca ccatcgagaa cacgcatgga acgttctact tcgattgtga agtcaacgtg
     2281 ccccggggtg tcgatgatgt tgatgcgatg cggctcatac tgcttagcca taccagacca
     2341 gaatgcagta gtcgcagcgg aagtgatggt aataccacgt tcctgctcct gctccatcca
     2401 gtccatggtt gcagcgccgt catgaacttc accgatttta tggtttacac cggtgtagaa
     2461 cagaatacgt tcggtagtag tggttttacc ggcgtcgatg tgcgcactga taccgatgtt
     2521 acggtagcgt gcgatgggtg ttgtacgagc catttgtttc ctcgtttatc ttttaggcgt
     2581 tcaatttaag tagcccaaag cgggctgctt actggaagcg cccgcctggt gactaaaact
     2641 ccgaagggat taccaacggt agtgtgcgaa cgccttgttg gcttcggcca tacggtgaac
     2701 gtcttcacgt ttcttaactg cagtaccttt gttttctgca gcatcagaaa gttcgttcgc
     2761 caggcgcaga gccatggatt tatcaccgcg tttacgagca gcttcaacga tccaacgcat
     2821 tgccagagca ttacgacgaa ccggacggac ttcaactggt acctgataag tagaaccacc
     2881 aacgcggcga gacttaactt ctacagtcgg gcgcacgttt tcgagagcta cttcgaatgc
     2941 ttccagttca gatttaccag agcgctgagc cagggtctcc agcgcgctgt atacgataga
     3001 ttcagcagta gattttttac catctaccat caggatattt acaaatttag ccagcagttc
     3061 tgatccgaac ttcggatccg gcagaatttt acgctgacca atgacgcgac gacgtggcat
     3121 ggaaatactc cgttgttaat tcaggattgt ccaaaactct acgagtttag tttgacattt
     3181 aagttaaaac gtttggcctt acttaacgga gaaccattaa gccttaggac gcttcacgcc
     3241 atacttggaa cgagcctgct tacggtcttt aacgccggag cagtcaagcg caccacgtac
     3301 ggtgtggtaa cgaacacccg ggaggtcttt aacacgaccg ccacggatca ggatcacgga
     3361 gtgctcctgc aggttgtgac cttcaccacc gatgtaggaa gtcacttcga aaccgttagt
     3421 cagacgaaca cggcatactt tacgcagcgc ggagttcggt tttttaggag tggtagtata
     3481 tacacgagta catacgccac gtttttgcgg gcatgcttcc agcgcaggca cgttgctttt
     3541 cgcaactttg cgagcacgtg gtttgcgtac cagctggtta actgttgcca ttaaatagct
     3601 cctggtttta gcttttgctt cgtaaacacg taataaaacg tcctcacaca atatgaggac
     3661 gccgaatttt agggcgatgc cgaaaaggtg tcaagaaata tacaacgatc ccgccatcac
     3721 caggccatct ggctggagtg cttaaccgta agtctgacga aatcagtata gtcaatggga
     3781 atgatgtcgt tcaaaatttg aacagtcaaa ccgcgggcaa taaggtcttc gttcagggca
     3841 tagaccttaa tgggggcatt acgcagactt tcaaggtagc ggttaccgtc aactgcggca
     3901 gttacgccat cttgcaataa tagcagttcg tctccttcac tgagcagacg cagcagcgca
     3961 gcaaaatccg tcagccaggg tgaacgatgt aacgtgtgca gcataaacgc ctcaaaacct
     4021 caaaataaca tcgtagttgg cgagttcgcg gcgtaaggca tctgcttcga gcggcgtggc
     4081 ttcgacaaca aagggtgtct gcggatctaa cccgcgttcg cgcagtgaag ccgcacaaac
     4141 ccagcactgt tcaatgtcgt acagactcaa caatttaaaa gtggcaatgt aatcacgcgc
     4201 cagcactgca tcgggctttt gtcctgacag cagctgaaaa acgccatcag caataaagaa
     4261 gacggccaga tcgtcagtta atgcggaagt tgccagtaaa gcatctaaac cttcccgacc
     4321 agcagctgta ccatgaggtg cagtagaaaa aacaaacgca attcgtttca tcagaactgt
     4381 accaccctgt cacaggtcag cgaggcttcc gccagcgcgc caagtccgct taaggtaaat
     4441 cccggctgaa ggtttgacga agccagcccc agccttcctg cttcagtttc atcgacgata
     4501 ccgcggcgta acgctgccgc tacgcagata ttcagcgcca caccatgttg cgcattcaac
     4561 tgttgccagc cgcgcacaag gtcaaattcg tcgcttgccg gtgaggtcag ttggttagcg
     4621 ttatagaccc cttcccgata gaaaaagacg ctgcttaact tatggccttc tgctatcagc
     4681 gcctgcgcaa actgaaaagc actactcgcc tgttgcgtac cgtatgctgg cccggtcacc
     4741 acgatggcaa aacgcattac ttatcttgcc cctgaaaatc accgctcttg aactggcgga
     4801 tgtagagata gacagtgtgt ttggagatgt tcaggcggtc agcaacctgg ttgatcgcat
     4861 ctttaatatc gaagatccct ttctcgtaga gattcagcac gatctgacga tttttggcgt
     4921 tattagaaac attgcgatcg gcattcactt cttcgatggt gaactccagc gtttgggtaa
     4981 ccagatcttc aacagaagag gcaaagttga cgcttgaacc gacatccggg gtttctggcg
     5041 gcacaaaagt gctcataatc tgcgagaagg gaacatcaag attcatattg atgcacagca
     5101 gaccaattac acgctgttcg cggttacgaa tcgcgatagt cagggacttc attaatacgc
     5161 cgcttttggc gcgagtaaag tagcatttag aaacgctgct atccgctccc gtcatatcgt
     5221 gcagcatacg tagcgccagg tcagtaattg gcgaaccaat cttccggcct gtatgttcac
     5281 cgttagcaat gcgaatggct gaacatttta gatcctgcaa agagtgcaaa acgatttcac
     5341 agtgggagcc aataagcatc gctaacccgt ccaccaccgc ttcgtaggat ttcagaatat
     5401 caaaatcggt ctggtcgaaa ggacgttgat ccagtaaatc caactcactg gtttcgttgg
     5461 ttaaaagcga cctggacatg aaaaaaaaca ctccttttca ggagcctgtc gttaactttt
     5521 cagggcaggc tcattaatga tgcgggtaac taaattaata cagcggaggt tccgctttcc
     5581 agcactaatt atatccggcc tgtaataaaa aaaccgccgc ctggtcaggc ggcggttctt
     5641 aatgcttatt ttttagcaga atctgcggct ttcgcatcag cttccggctt tgcatcagcc
     5701 ttcggcgctg gtttcacatc cagcagctct acgtcaaaca ctagggtaga attcggtggg
     5761 atccccggaa cacccgcttt gccgtaagcc agttctggtg gaataaccag tttgatctta
     5821 ccgcctttct tgatgttctt cagaccttct gtccaacccg ggataacacc gtccagacgg
     5881 aaagaaagcg gttcaccacg ggtgtaagag ttgtcgaact ctttaccgtc gatcagcgta
     5941 cctttgtagt tcactacaac agtatcgctg tctttcggtg cttcgccttt accggcttct
     6001 actacctgat aaaccagacc agttgaagag gttttcacac ctttctcttt ggcaaatttc
     6061 tcgcggtact ctttaccttt tgcttcgtta tcagccgcgt ctttttccat cttcgcctga
     6121 gcagaagact tcacgcgagc ttcgaatgct tgcagagtct gttcgatctc ttggtcggag
     6181 agtttgctct tatcagcaaa tgcatcctga acaccagcga tcagctgatc tttatccagt
     6241 ttgatgccca gtttttcttg ttctttaaga gagttttcca tgtaacgacc cagcgaagca
     6301 cccagtgcat aagctgattt ctgatcgtca tttttgaacg ctgctttgct gtcagcagtt
     6361 gtagcaggtt ttgcagcttc agcagcaaaa gtgattggtg catgcagggc aacggccatt
     6421 gtggtcgcca gtagcgttac tttaaacagt gatttcatcc atatctccag gatcggggca
     6481 tctcacccca gggttaacta ttatcagaag ggtactataa agcgttgtcg aacaaatcta
     6541 catacagaca cgccctatta tcatctattt tcagactctt tttgtttaaa ttagtttcga
     6601 tgaccgcgaa atgagtgctg tctcgggcag caaagttaag tagaatccgc ggcgaccatt
     6661 cgacaaaaga ggtgaatcat gcaggattta tcattggaag cacgcctggc agagctggag
     6721 agccgactgg cttttcagga aatcaccatt gaagaactga acgtcacggt gaccgctcat
     6781 gaaatggaga tggcgaaact gcgcgatcat ctgcgtctgc tgaccgagaa gttaaaagcc
     6841 agccagccgt cgaacatcgc gtcgcaggct gaagaaacgc caccgccaca ttattgaggc
     6901 gtaaaaaaag cggggattcc ccgctttttt gtcacctttt cggtattagt ggcaaccaca
     6961 accgccgttg cctttaccgc cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg
     7021 gccatgaccg ccgcagcaac cgtcgtggtc gtgatcgtgg tgatgatcgt gcgcgccgtg
     7081 aacgtggcca tgagccagtt cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt
     7141 gaatttcagg ttctgaccgg ccagcatgtg gttaccatca accactacgt gatcgtcttc
     7201 aaccgcagtg atttcaaccg gtaccggacc ctgctcagtt tcagccagga aacgcatacc
     7261 tacctgcagt tcatcaacgc ccataaatac gtctttagga acacgttgca ctaggttttc
     7321 gtcgtactga ccgtaagcgt cgttcgcgcc aacagcgaca tcaaatttgt cgccaacttc
     7381 atgaccttcc agcgccgttt ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc
     7441 cagcggcgca ctcaccggag actcatcaac caacacaccg tcttctgtac gtacctgata
     7501 ggccaggctg accaccaggt cttttgctac tttcatgata tctcctgagc atgggaagaa
     7561 tagtggcgca gattgtagcg gaattctgca gccgtgtact cactagctta aaaaaactcg
     7621 gcgcatatcg ctagtccgga tgaaaaatcc cgatcacttg ctcatctttg tgaacgtgat
     7681 cgcgggcttc tttgtctgct tctcgcatct gatgtccgca cttaacacat tcaacaatat
     7741 caatattatt ttcgcgccac atcgccattg aatcctgcgc ctgacaggcc gggcattttg
     7801 cgcccgctat aaaacgtttt cggattgcca tccttaccct ctactcaaat tcatcccagc
     7861 cgtccagctg gcgtcgttct tgttgcattt cacgctggaa aatctcttcc agttcgcgtc
     7921 gggcttccct ggcgcgagaa atttgtacgg tatcagcatg catcgggatt aactcccgca
     7981 gcattcgcat atccaggcgg cgaaaatgca gttgcgcccg ctgtgcctga tgcggatgca
     8041 tgccaagcgt gaccagcgtc ttgcgcccca gctctaacgc actggagaat gtttcacggg
     8101 aaaactgcgt cacccctgcc tgtaataact catgcgcttc cacacgtccg cgcactcgcg
     8161 caagaatatg caaatgcgga aagtgctgct ggcatatttc caccagcttc atggtgtctt
     8221 ccggctcgtt acaggtaatg acgatagact cagcggcctc tgcacccgca gaacgtaaaa
     8281 gatcgacctg cgtggcgtcg ccgtaataaa ctttgtagcc atatttacgc atcaggttaa
     8341 cggcgctgat atcccgctcc agcacggtaa tgcgcatttt atttgccatc agcaaacgac
     8401 caatcacctg accaaaacgc ccgaagccca cgacaatgac ctggggttta tcatcgttga
     8461 cccacggttt ttcgtcttct tcttccggtc cgttaaactg gcgggatagc catttatcca
     8521 ccagcttcat cagcaacggc gtggtcatca tggaaagcgt caccgtcacc agcaacagcg
     8581 ccatctggtc gccctggaat aaacgttgtg aagaagcggt agaaaagagg acaaaggcaa
     8641 actcaccgcc ctgactcaac acgccagcaa actgcatccg ctctgagcta cgcacgccat
     8701 acaatcgcgc cagcagatac agcacgagaa ttttcaccgc caccagcaca accacgctta
     8761 tcactaccca caacagatgg gtataaagca ccccgaggtt gagcgacatg ccgacagaga
     8821 taaagaacaa accgagcagc aagcctttga agggatcgat agccgtttcc agttcatggc
     8881 gatattcact ttccgccagc agcacgcccg caataaatgt accgagcgcc atcgacagcc
     8941 ccagcgcatc cataaacaat gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca
     9001 cttcccgcac gccagaagct gcaataaagc ggaataccgg acgcagtaaa tagcgcccac
     9061 caatcagcat gccgacaaac gccagcacct tcatgccgat cttcatccag tcgaaatgtt
     9121 cgtctgccga ccccgccaac aacggcacta acgccagtgc tgggatcact gccagatcct
     9181 gaaacagcag aaccgaaaac ccgagctggc cggattcgct gcgattcatc cctttctcac
     9241 gcatcaattg caacgccatt gcagttgaag acatcgcaag gccaatgcca ccgaccaccg
     9301 ccgcctgcca ggcgaaatcc gtcagcatca ataatcccgc cagcaacgcc gcgcttaaca
     9361 gcacctgtgc cacgcctacg ccaaaaatcg aacgccgcag ttgccaaagt ttggagggat
     9421 tcaactcaag gccgatgata aacatcagga ataccacgcc gagttccgaa aagtggagga
     9481 tctcatcgac gtcgctaata aaccccagcc cccacgggcc aattgcaatc cctgccagca
     9541 aatatcccaa cacagcgcca atccccagcc gcgatgccaa cggcaccgca gccaccgccg
     9601 cgaagagaaa cagcactcct gcgagtaaaa aatcggaacc ttccatcagc ggcctcctgg
     9661 agacagcgga tttgccaacc agtcaccgta ggctctggcg tggctcgcca gctcctttgc
     9721 gctttgccgt ctcgcccagt aaataatgat ggaacttaac caatgcatcc ggcacatgcc
     9781 cgccgccagt tcaaaggggc gcagcacatc gctcatcggg tagcgattca gcgcgtcata
     9841 acggtaagca ctttccggct cgccggtggt aatcacgtta cgccagtact ttcccgccag
     9901 ttggtttcct cccggcccgc tggcaaaacc acgacttaat acccggtcca gccactcttt
     9961 cagtagcgcc gggcagctat aggtataaag aggatgctga aagacaatca cctcgtgctc
    10021 gcgcagtaat gcctgctcac gggggatatc aataaaaaaa tcgggatagt gcgcgtaaag
    10081 gtcgtgcacg gtaacattgc tgagctgcgt ggccggttta agcagtaccc ggtttgccac
    10141 cgagtcctga gattccggat gggcatacag cagcaaaact ttcgctggct gagacatcat
    10201 ccccctcccg gtatggtttt tgtgtatagt cgctgttttg ggctaccatt gcgcccggtg
    10261 cggcagctcg cccatacatt acattatcat aatgataagt taacatagtc tgaacatacg
    10321 gcgccttatg attgttttct cctcgttaca aattcgtcgc ggcgtgcgcg tcctgctgga
    10381 taatgccacc gccaccatca accccgggca gaaagtcggc ctggtgggta aaaacggctg
    10441 tggtaaatct accctgctgg cattgctgaa aaatgaaatc agcgccgacg gcggcagcta
    10501 cacctttccg ggaagctggc aactggcgtg ggtgaatcag gaaacgccag cgttaccgca
    10561 agcggcgctg gaatatgtca ttgacggcga ccgtgaatat cgtcaactgg aagcgcagct
    10621 gcacgacgcc aacgaacgta acgacgggca cgccattgcg accattcata gcaagctgga
    10681 tgctattgac gcatggagta ttcgctcccg tgccgccagc ctgctgcacg gcctcggttt
    10741 cagcaatgaa caactggagc gcccggtaag tgatttctcc ggtggctggc gtatgcgtct
    10801 taaccttgcc caggcgctga tttgccgttc agacttgctg ctgctcgacg aaccgactaa
    10861 ccacctcgat ctcgatgccg ttatctggct ggaaaaatgg ttgaagagct atcagggcac
    10921 gctgatcctg atctctcacg accgcgactt cctcgatccg atcgttgata aaattattca
    10981 gatcgaacaa caaagcatgt tcgagtacac cggcaactac agttcgtttg aagtacagcg
    11041 cgccacccgt ctggcgcagc aacaagcgat gtacgaaagc cagcaggaac gcgtggcaca
    11101 tctgcaaagt tatatcgatc gtttccgtgc caaagccacc aaagcgaagc aggcccagag
    11161 ccgcattaag atgctggaac gtatggagct gattgccccc gcacacgtcg acaacccgtt
    11221 ccgctttagc ttccgcgcgc cggaaagcct gccaaatccg ttactgaaga tggaaaaggt
    11281 cagcgcaggc tatggcgatc gcattattct cgactcgatt aaattgaatc tggtccccgg
    11341 ctcgcgcatt ggtctgttag gccgcaacgg cgcaggtaaa tcgacattaa tcaaactgtt
    11401 agccggtgaa cttgcgccag tcagcggtga aattggtctg gcaaaaggga tcaagctcgg
    11461 ctacttcgcc cagcatcaac ttgaatacct gcgcgccgac gaatcaccta ttcaacatct
    11521 ggcacgttta gcgccgcagg agctggaaca aaaactgcgt gactacctcg gcggctttgg
    11581 tttcctgggc gataaagtaa ccgaagaaac gcgccgcttc tccggtgggg aaaaggcccg
    11641 cctggtgctg gcattaattg tctggcagcg tccgaatctg ctgctgctcg acgaaccgac
    11701 taaccacctt gacctcgaca tgcgtcaggc agtcaccgaa gcattaatcg agtttgaagg
    11761 cgcgctggtt gtcgtttcgc acgaccgtca tttgctgcgt tccaccactg acgatctcta
    11821 cctggttcac gatcgtaaag tcgaaccgtt cgacggcgat ctggaagatt atcaacagtg
    11881 gttgagcgac gtacaaaagc aggaaaacca gaccgacgaa gcgccaaaag agaacgcgaa
    11941 cagcgcccag gcacgtaaag atcagaagcg tcgggaagcg gagctgcgtg cgcaaaccca
    12001 gccgctgcgt aaagagattg cccgtctgga aaaagagatg gagaagctga acgcgcaact
    12061 ggcgcaggcg gaagagaaac tcggcgacag cgaactgtat gaccagagcc gtaaagcgga
    12121 gttgaccgcc tgcctgcaac agcaagccag cgccaaatcc ggcctggaag agtgcgaaat
    12181 ggcgtggctg gaagcccagg agcagcttga gcagatgctg ctggaaggcc aaagcaactg
    12241 atggcgcaga taacgacgac cgatgccaat gaatttagca gcagtgctga attcacccct
    12301 atgcgcggct ttagcaattg tcatctacaa actatgctgc cgcgtttgtt tcgtcgtcag
    12361 gtgaaattca ccccgtactg gcagcggctg gagttgcccg acggcgattt tgtcgatctc
    12421 gcgtggagtg aagaccccgc acaggcgaaa cataaaccgc gtttagtggt gtttcacggg
    12481 ctggaaggca gtctcaatag cccttacgcc cacggtctgg ttgaggcggc gcaaaagcgc
    12541 ggctggctgg gcgtggtgat gcattttcgc ggatgcagcg gtgaaccaaa ccgtatgcac
    12601 cgcatttacc attcgggcga aaccgaagac gccagttggt ttttacgctg gctgcaacgc
    12661 gaatttggtc atgcgccaac ggctgccgtc ggctattcgc tcggcggtaa tatgctggcc
    12721 tgtttgctgg caaaggaagg caatgatctc ccggttgatg cggcggtgat tgtctctgcg
    12781 ccgtttatgc tggaagcctg tagctatcat atggaaaagg gcttttcccg cgtttatcag
    12841 cgttacttgc tgaacctgtt aaaagccaat gccgcgcgca agctggcagc ctaccccgga
    12901 acgctgccga ttaatctcgc gcagttaaaa tcggtacgtc gcatccgtga atttgacgat
    12961 ctaatcaccg ccagaattca cggctacgcc gacgctatcg actattatcg tcagtgtagc
    13021 gccatgccga tgctgaaccg gatcgccaaa ccgacgctga ttattcacgc caaagacgat
    13081 ccgtttatgg atcatcaggt gatcccaaaa ccggaaagtc tcccctcgca ggtggagtat
    13141 caactgactg aacatggcgg tcatgttggc tttattggcg gtacattact tcatccgcaa
    13201 atgtggctgg agtcacgcat tcctgactgg ttaacaacgt atctggaggc gaaatcatgt
    13261 tgattccgtg gcaagacctc tcccccgaaa cgctggaaaa tttaattgaa agctttgtgt
    13321 tacgtgaagg caccgattat ggtgaacatg agcgtacact tgaacagaaa gtcgccgacg
    13381 tcaaacgcca gctacagtgc ggagaagcgg tgctggtatg gtcggaactg cacgaaacgg
    13441 tcaatattat gccgcgcagt cagtttcgcg aataacctgc aaccgtcggc ggacgagata
    13501 atagtgatag ctaagtcatg gagttactat gtctgccaaa catccggtca ttgcggtaac
    13561 aggatccagc ggcgcgggga ccaccaccac cagcctcgcg tttcgtaaaa tattcgcgca
    13621 gttaaatctg catgcagctg aggtggaagg cgacagtttt caccgttaca cccgcccgga
    13681 aatggacatg gcgatccgca aagcgcgcga cgccgggcgg catatcagct acttcggccc
    13741 cgaagcgaac gacttcggcc tgctggaaca aaccttcatt gaatacggtc agagcggcaa
    13801 agggaaatct cgcaaatatc tgcataccta cgacgaagcc gtaccctgga atcaggtacc
    13861 gggaacattc accccctggc aacctttacc agaacccact gatgtactgt tttatgaagg
    13921 tttacacggc ggcgtagtca cgccacagca taacgttgcg cagcatgtgg acttactggt
    13981 cggcgtagtg cctatcgtta accttgagtg gattcaaaaa ctgatccgcg acaccagcga
    14041 gcgcgggcac tcacgagaag cagtgatgga ctcggtagtg cgttcaatgg aagactatat
    14101 caactacatc acaccgcagt tttcccgcac ccatcttaac ttccagcgcg ttcccaccgt
    14161 cgacacttca aacccgttcg cggcaaaagg tatcccgtcg ctggatgaaa gctttgtggt
    14221 gatccatttt cgtaatctgg aagggatcga tttcccttgg ctgctggcga tgttgcaagg
    14281 ctcattcatt tcccacatca atacgttagt ggtacctggc ggcaaaatgg ggctggcaat
    14341 ggaattaatt atgctgctgc tggtgcaacg attgatggaa ggaaagaaaa tcgagtaact
    14401 ttgctattat gccggataaa atactatccg gcttcacaac gggatagtta agtcacgcgg
    14461 caaccacttc atacgagtga gtaatattca ccgctttttc cagcatcaac gccactgaac
    14521 aatatttctc ggcagagaga tcaaccgcac gcgcaaccgc tgcgtctttc aggtcgcgac
    14581 cggtgacgat aaaatgcaga ttaatgtgcg taaacaggcg tggtgcctct tcgcggcgtt
    14641 cagaggtcaa ttttacttca caatcgacca catcctgacg ccctttttgc aggatcgaaa
    14701 ccacatcgat ggcactgcaa ccacccgccg ccatcaacac catttccatc ggacttggtg
    14761 ctttatcgcc tgagttgccg tccattaaaa tctggtgacc agaggcggat tcgcccagaa
    14821 aagttaaccc ttcgacccac ttcactcgcg cttgcatttt tgctactcca ctgcgtcaat
    14881 tttcctgaca gagtacgcgt actaaccaaa tcgcgcaacg gaaggcgacc tgggtcatgc
    14941 tgaagcgaga caccaggaga cacaaagcga aagctatgct aaaacagtca ggatgctaca
    15001 gtaatacatt gatgtactgc atgtatgcaa aggacgtcac attaccgtgc agtacagttg
    15061 atagcccctt cccaggtagc gggaagcata tttcggcaat ccagagacag cggcgttatc
    15121 tggctctgga gaaagcttat aacagaggat aaccgcgcat ggtgcttggc aaaccgcaaa
    15181 cagacccgac tctcgaatgg ttcttgtctc attgccacat tcataagtac ccatccaaga
    15241 gcacgcttat tcaccagggt gaaaaagcgg aaacgctgta ctacatcgtt aaaggctctg
    15301 tggcagtgct gatcaaagac gaagagggta aagaaatgat cctctcctat ctgaatcagg
    15361 gtgattttat tggcgaactg ggcctgtttg aagagggcca ggaacgtagc gcatgggtac
    15421 gtgcgaaaac cgcctgtgaa gtggctgaaa tttcgtacaa aaaatttcgc caattgattc
    15481 aggtaaaccc ggacattctg atgcgtctgt ctgcacagat ggcgcgtcgt ctgcaagtca
    15541 cttcagagaa agtgggcaac ctggcgttcc tcgacgtgac gggccgcatt gcacagactc
    15601 tgctgaacct ggcaaaacaa ccagatgcta tgactcaccc ggacggtatg caaatcaaaa
    15661 ttacccgtca ggaaatcggt cagattgtcg gctgttctcg tgaaaccgtg ggacgcattc
    15721 tgaagatgct ggaagatcag aacctgatct ccgcacacgg taaaaccatc gtcgtttacg
    15781 gcactcgtta atcccgtcgg agtggcgcgt tacctggtag cgcgccattt tgtttccccc
    15841 gatgtggcgc agactgattt atcaccccga tatcaactat gcacttcgac aaacgctggt
    15901 gctatgtttg cccgtggccg ttgggttaat gcttggcgaa ttacgattcg gtctgctctt
    15961 ctccctcgtt cctgcctgtt gcaatattgc gggccttgat acgcctcata aacgtttttt
    16021 caaacactta atcattggtg cgtcgctgtt tgccacctgt agcttactga cacagctgct
    16081 actggtaaaa gatgttcccc tgcccttttt gctgaccgga ttaacgctgg tacttggcgt
    16141 cactgctgag ctggggccat tgcacgcaaa attgcttcct gcatcgctgc tcgccgccat
    16201 ttttaccctc agtctggcgg gatacatgcc ggtctgggaa ccattgctca tctatgcgtt
    16261 gggcactctc tggtacggat tgtttaactg gttttggttc tggatctggc gcgaacaacc
    16321 gctgcgcgag tcactaagtc tgctgtaccg tgaactggca gattattgtg aagccaaata
    16381 cagcctgctt acccagcaca ccgatcctga aaaagcgctg ccaccgctgc tggtgcgcca
    16441 gcaaaaagcg gtcgatttaa ttactcagtg ctatcagcaa atgcatatgc tttccgcgca
    16501 aaacaatacc gattacaagc ggatgctgcg tattttccag gaggcgctgg atttgcagga
    16561 acatatttcg gtcagtttgc atcagccgga agaggtgcaa aaactggtcg agcgtagcca
    16621 tgcggaagaa gttatccgct ggaatgcgca aaccgtcgcc gctcgcctgc gcgtgctggc
    16681 tgatgacatt ctttaccatc gcctgccaac gcgttttacg atggaaaagc aaattggcgc
    16741 actggaaaaa atcgcccgcc agcatccgga taatccggtt gggcaattct gctactggca
    16801 tttcagccgc atcgcccgcg tgctgcgcac ccaaaaaccg ctctatgccc gtgacttact
    16861 ggccgataaa cagcggcgaa tgccgttgct tccggcactg aaaagttatc tgtcactaaa
    16921 gtctccggcg ctacgcaatg ccggacgact cagtgtgatg ttaagcgttg ccagcctgat
    16981 gggcaccgtg ctgcatctgc cgaagtcgta ctggatcctg atgacggtat tgctggtgac
    17041 acaaaatggc tatggcgcaa cccgtctgag gattgtgaat cgctccgtgg gaaccgtggt
    17101 cgggttaatc attgcgggcg tagcgctgca ctttaaaatt cccgaaggtt acaccctgac
    17161 gttgatgctg attaccaccc tcgccagcta cctgatattg cgcaaaaact acggctgggc
    17221 aacggtcggt tttactatta ccgcagtgta taccctgcaa ctgttgtggc tgaacggtga
    17281 gcagtacatc cttccacgtc ttatcgatac cattattggt tgtttgattg ctttcggtgg
    17341 taccgtctgg ctgtggccgc agtggcagag cgggttattg cgtaaaaacg cccatgatgc
    17401 tttagaagcc tatcaggaag cgattcgctt gattctgagc gaggacccgc aaccaacgcc
    17461 actggcctgg cagagaatgc gggtaaatca ggcgcataac actctgtata actcattgaa
    17521 tcaggcgatg caggaaccgg cattcaacag ccattatctg gcagatatga aactgtgggt
    17581 aacgcacagc cagtttattg ttgagcatat taatgccatg accacgctgg cgcgggaaca
    17641 ccgggcattg ccacctgaac tggcacaaga gtatttacag tcttgtgaaa tcgccattca
    17701 gcgttgtcag cagcgactgg agtatgacga accgggtagt tctggcgatg ccaatatcat
    17761 ggatgcgccg gagatgcagc cgcacgaagg cgcggcaggt acgctggagc agcatttaca
    17821 gcgggttatt ggccatctga acaccatgca caccatttcg tcgatggcat ggcgtcagcg
    17881 accgcatcac gggatttggc tgagtcgcaa gttgcgggat tcgaaggcgt aatacaggct
    17941 aaaccatcgg ataaggcatt cacgccgcat ccgacatatt ttgcctgatg cgtcttattt
    18001 acgccccaac caccttcgcc accgcgtggg cgaaacgttg catcccctca tcgatatccg
    18061 catcttccac caccagcgac ggtgctaaac gcatcacatc cggtccggca ttcagcacca
    18121 ttacgccaac ctctgcaccc gcatacagga aatcacgcgc ccgacctttg tagtgtggtt
    18181 tcagctccgc gccaattaac agccccatac cgcgaatatc gctaaacaca tcgtactgct
    18241 gatcgatctt ctgcaaatgg tcaacaaaac gctggcgttt cgcctgaatg ccttccagca
    18301 cttcaggggt attgatgata tcaaacgccg cgcccgctac tgcacaggcc agaggattac
    18361 cgccgtaggt ggaaccgtga gaaccaggat gaaacgcaga agcaatttcc gccgtggtca
    18421 gcatggcgct aatcgggaag ccgccgccta acgctttcgc agaagtcaga atatccggcg
    18481 tcacaccgta gtacctgtaa gcaaacaaat cgccagtccg ccccatcccg cactgcactt
    18541 catcaaacac caataatacc tgatgttgat cgcacagctc gcgcaagccc tgcaaaaact
    18601 ctggcgtcgc tgccgtcaca ccgccctcgc cctggatcgg ctcaaccacc accgcacagg
    18661 tgtgatcatc catcaccgct ttcactgcat ggagatcgtt aaagggaacg tggatgatgt
    18721 ctgccggttg cggcccaaag ccgtcggaat attttggctg cccacccacc gaaacggtaa
    18781 acagcgagcg accatgaaaa gcgttatgga aggcaataat tttggttttg aacgggctat
    18841 gacgcacaca ggcgtaatgg cgtgccagtt taaaggcggt ttcgttagct tccgtgccgg
    18901 agttcataaa caccacgcgt tcggcaaacg tcgcctcaat cagtttgcgc ccaagacgca
    18961 gcgccggttc attggtgaaa acgttactga tatgccacag agtttcgccc tgggttttta
    19021 acgcgttcac cagcgcagga tggcaatggc ccaacgccgt aactgcaatg ccacccgcga
    19081 aatcgacata ctccttgcct tgctgatccc agattcggct gccctgacct tttaccggaa
    19141 taaactctgc cggagcataa atcggcagga tcacttcatc gaaagtcgcg cgtgtaattg
    19201 ctgtttgttc aattgccatc tcatgatcac cctgttacgc ataaataaat atgaaattat
    19261 aatcacaaaa tatgcataaa aaatcactga atggcaatcg gaaatcagag atgcaggaaa
    19321 ttagccagca gttgatgtcc ttgttcgcta agaatacttt ctggatggaa ctgcacacct
    19381 tccagatccc actggcgatg gcgaatcccc ataatctcgc gggtttcgct ccaggccgtc
    19441 acgtcaaagc acgctggtaa tgagtcaggt tccaccacca gcgaatggta gcgtgtcacg
    19501 gtaagtggat ttgccagccc ccgaaatacg ccctcaccgc tatgtgtaat cggcgaggtt
    19561 ttgccgtgca tgacctttgc ggcgcgcaca actttgccgc caaatgcctg cgccattgcc
    19621 tgatgaccga ggcagacgcc aagaatcggc aagcgcccgg catagtggcg aataacgtca
    19681 agggagatcc cggcttcatc tggcgtacag gggccaggtg agatgacaat tttttgtggt
    19741 ttaagggcgt cgatatccgc cagcgtcaac gcatcgttgc gcttaaccag cacatccgcc
    19801 cccagttcac aaaagtactg gtagaggttc caggtaaaag aatcgtagtt atctataagc
    19861 aggatcatgg cggctccggt acgaaagaac cgcactattt tactcagatt ccccggcttc
    19921 gcttaccact ttgctaaata tcatctgcag tgcggtgaga tcccccattg ccccactctg
    19981 atttgcctga ttccaagcct ctttttcgat accctgccag ctcagttgat atccggcatg
    20041 aatcgccagt tgctcgaaga agatccgctg tgccagacca cttcccaccc ggaagggatg
    20101 cagcacgtcg atttcacaat agtaatgcgc cagccgctcg acgaacttcg ctttctccag
    20161 gccaaccaga taaccttctt cctccagatc ctgcatcagg gcattgccct ctttttcgat
    20221 ataagcaaag tggcagaacg gcgtatcgcc ctgataaata tcaacttcac gcagttgccc
    20281 tgcccagtcg aaaatatcct ggtacagctg gcgatggata gtgcgcaaat gcggtaaacc
    20341 gcgcaccagc ggaccaagct caatggtcgc agcacgcagc gccgtcattt cgtaagcggc
    20401 ctgttccagc cgctgctgct ggcggatgtt cagccggtta cgcatgatat caaggccagg
    20461 ataaagatac ggatcgcgcc cttcgccgaa tttatcgctc atagtgcctc ctcagctctt
    20521 caaggcgcgc taaagcctct gcggcagtaa gagtgactaa aggcatctcg acgccttcaa
    20581 gacggcgact ggcctggaaa ttacgattac gctgaagctc ccagagacgg gacttttgct
    20641 tatcggtgag tttcttcact tgatgcctcc ctgaacgtgt ccatttgcca caagtataag
    20701 cggcaaatcc gggttacgcc gggagagcag aagcgcgggc aagattgccc gcgcgagaaa
    20761 tcattacggc aggactttag cggaaaggat aactaccggt tttgacggca cattctggta
    20821 cggaccaaca tcatgagtcg gcacctggga aatcttatcg gcaacgtcca tgcctttcac
    20881 cactttacca aataccgcgt aaccgaaatc acgctgacca tggtcaagga aggcgttatc
    20941 ggcaacgttg ataaagaact ggctggtggc gctgtctttg tcagcggtac gcgccatcgc
    21001 gatggtgcca cgcgtgttgc gcaggccgtt atcggcttca tttttgattg gcgggtttgg
    21061 ttttttctgc tgcatctgct cggtgaaacc gccgccctga atcataaagc caggaatgac
    21121 gcggtgaaag gtagtgttgt tataaaaacc gctgttcaca taatcgacaa agttttgcac
    21181 agacactggc gctttttgtt tatccagctc cagttcgatg ttaccagctg aggttgtcaa
    21241 caatacgtgc gggtcccctt tcgctgccat tgctgcggga gaaagagcag aaagagcgaa
    21301 aacagcagcc atcgccgcca gggtcgattt gaacatgaga tttccttaac aaacagagca
    21361 gaaaaaaagc aagtgaagag attctaaata gccttcaaga acaaggccat ccctttacct
    21421 aattttacgt atctgaaata tatgtaacct gacgatcact ttttattccg taatcaaaat
    21481 cacctcttaa aatgcaattt agcaaccgat tgcaataaaa catttaaaca gatcacaaaa
    21541 tcacctaaaa tcgcccgctc gcggcatatc ccgctatgcc attttatttt tgaacgcagg
    21601 ccaatcatga ctaacagcaa tcgcatcaag ctcacatgga ttagctttct ctcctacgca
    21661 ctgactggtg cgttggttat tgtcaccggg atggtgatgg gaaatatcgc cgattatttc
    21721 aatctgcctg tttccagtat gagtaacacc ttcaccttcc tcaacgccgg cattttaatc
    21781 tctatcttcc tcaacgcctg gctgatggaa atcgtcccgt tgaaaacgca gttacgtttt
    21841 ggctttctcc tgatggtgct ggcggttgcc ggtttgatgt tcagccacag cctggcgctg
    21901 ttctcgacgg cgatgttcat tctcggggtg gtcagcggca ttactatgtc gattggtaca
    21961 ttcctgataa cacaaatgta tgaagggcgt cagcgcggtt cccgcctgtt atttactgac
    22021 tccttcttca gtatggctgg gatgattttc ccaatgatcg ccgcgtttct actggcgcgc
    22081 agcattgagt ggtactgggt ttatgcctgc atcgggctgg tgtatgtcgc tatttttatt
    22141 ctgaccttca gctgtgagtt cccggcactg ggcaaacgtg caccaaaaac ggatgctccg
    22201 gtagagaaag aaaagtgggg gatcggcgta ctgtttctct ctgttgcggc gctgtgctac
    22261 atcctcggtc agttaggttt tatctcctgg gtgcctgagt atgccaaagg cctgggcatg
    22321 agcctgaacg acgcgggcac gctggtgagt aacttctgga tgtcatacat ggtcggcatg
    22381 tgggcgttca gctttattct tcgcttcttt gatttgcaac gcattctgac cgtactggct
    22441 ggtctggctg cgattctgat gtacgtcttt aacaccggaa caccagcaca tatggcgtgg
    22501 tcaattctcg ctctgggctt cttctccagc gcgatctata ccaccatcat caccctgggt
    22561 tcacagcaga ccaaagtacc gtcgccaaaa ctggttaact ttgtcctgac ctgcggaacc
    22621 atcggtacta tgttgacctt tgtggttacc ggcccgattg ttgaacatag cggtccgcag
    22681 gcggcactgc ttacagcaaa cggtctgtac gctgtcgtct ttgtgatgtg cttcctgtta
    22741 ggtttcgtca gccgtcaccg tcagcataac accctgacct ctcattaatt gctaatgccg
    22801 gacggcacta tcgtcgtccg gccttttcct ctcttccccc gctacgtgca tctatttcta
    22861 taaacccgtt cattttgtcc attttttgca caaacatgaa atgtcagaca attccgtgac
    22921 ttaagaaaat ttatacaaat cagcaatata ccccttaagg agtatataaa ggtgaatttg
    22981 atttacatca ataagcgggg ttgctgaatc gttaaggtag gcggtaatag aaaagaaatc
    23041 gaggcaaaaa tgagcaaagt cagactcgca attatcggta acggtatggt cggccatcgc
    23101 tttatcgaag atcttcttga taaatctgat gcggccaact ttgatattac cgttttctgt
    23161 gaagaaccgc gcatcgctta tgaccgcgta cacctctcgt cttacttctc tcaccacacc
    23221 gccgaagagc tgtcgctggt gcgcgaaggc ttctacgaga aacacggcat caaagttctg
    23281 gtcggcgaac gcgctatcac catcaaccgt caggagaagg tgattcactc cagcgccgga
    23341 cgtaccgttt tttatgacaa gctgatcatg gcaaccggtt cctacccgtg gatcccacca
    23401 atcaaaggtt ctgatactca ggactgcttt gtctatcgca ccattgaaga cctcaacgcc
    23461 attgaatcct gcgcccgtcg cagcaaacgc ggtgccgttg ttggtggcgg cctgttaggt
    23521 ctggaagccg ctggcgcgct gaaaaactta ggtattgaaa cccacgttat cgaatttgcc
    23581 cctatgctga tggcggaaca gcttgatcag atgggtggcg agcagctgcg tcgcaaaatc
    23641 gaaagtatgg gcgtgcgcgt tcacaccagc aaaaacaccc ttgagattgt gcaggaaggt
    23701 gtcgaagcgc gtaaaaccat gcgttttgcc gacggcagcg aactggaagt cgactttatc
    23761 gtcttctcta ccggtatccg tccgcgcgat aagctggcaa cccagtgtgg tctggacgtt
    23821 gctccgcgtg gggggattgt cattaatgat tcctgccaga cttccgatcc ggatatctac
    23881 gccatcggtg aatgcgcaag ctggaacaac cgtgtatttg gtctggtagc accaggctac
    23941 aaaatggcgc aggtcgccgt tgaccatatt ctgggtagcg aaaacgcctt tgaaggtgct
    24001 gaccttagcg ccaagctgaa actgctgggc gtagacgtag gtggtattgg tgatgcgcac
    24061 ggtcgcacgc ctggcgcacg tagctatgtt tacctcgacg aaagtaaaga gatctacaaa
    24121 cgcctgattg tcagcgaaga caacaaaacc ctgctcggtg cggtactggt gggcgatacc
    24181 agcgactacg gcaacctgct gcaactggtg ctgaacgcta tcgagctgcc ggaaaacccg
    24241 gattctctga ttctgccggc gcactcgggt agcggtaagc cgtctatcgg tgttgataaa
    24301 ctgccggaca gcgcgcaaat ctgctcctgc ttcgacgtca ctaaaggtga tctgattgct
    24361 gccatcaaca aaggctgcca cacagttgcg gcgctgaaag ctgaaaccaa agcgggtact
    24421 ggctgcggtg gctgtatccc gctggtcact caggtactga acgcggaact ggcgaaacag
    24481 ggcatcgaag ttaacaacaa cctgtgcgaa cactttgctt attcgcgtca ggaactgttc
    24541 catttgatcc gcgttgaagg cattaaaacc ttcgaagaac tgctggcgaa acacggcaaa
    24601 ggctacggtt gtgaagtttg taaaccaacc gtcggttcgc tgctggcctc ctgctggaac
    24661 gaatacattc tgaagccgga acatactccg ctgcaggatt ctaacgacaa cttcctcgct
    24721 aacatccaga aagacggcac ctactcggtg atcccgcgtt ctccgggcgg tgaaatcacc
    24781 ccggaagggc tgatggcagt gggtcgtatc gcgcgtaaat ttaatctcta caccaaaatc
    24841 accggctccc agcgcctggc gatgtttggc gcacagaaag gcgatctgcc ggaaatctgg
    24901 cgtcagctga ttgaagctgg cttcgaaacc ggtcatgcct atgcgaaagc actgcgtatg
    24961 gcgaaaacct gcgtgggtag cacctggtgc cgctacggtg ttggcgatag cgtcggcctc
    25021 ggcgtggaac tggaaaaccg ctacaaaggc atccgtacgc cgcacaaaat gaagttcggt
    25081 gtctccggct gtacccgtga atgttcagaa gctcagggta aagacgtggg tattatcgcc
    25141 actgaaaaag gctggaacct gtatgtttgc ggtaacggcg gcatgaaacc gcgtcatgcg
    25201 gatctgctgg cagcggatat cgatcgcgaa acgctgatca aatatctcga ccgcttcatg
    25261 atgttctaca tccgtaccgc cgacaaactg acgcgtaccg cgccgtggtt agaaaacctc
    25321 gaaggcggca tcgattacct gaaagcggtg atcattgacg acaaactggg gctgaacgct
    25381 catctggaag aagagatggc gcgcctgcgt gaagcggtag tgtgtgagtg gactgaaacg
    25441 gtcaatacac cgtccgcgca gactcgcttc aaacacttca tcaacagcga caagcgtgac
    25501 ccgaacgtgc agatggtgcc agagcgcgaa cagcaccgtc cggcaacgcc gtatgaacgt
    25561 atcccggtaa ctctggtgga ggacaacgca tgagccagtg gaaagacatc tgcaaaatcg
    25621 atgacatcct gcctgaaacc ggcgtctgcg cgctgttagg tgacgagcag gtagcgattt
    25681 tccgcccgta tcacagcgac caggtgtttg cgatcagcaa cattgatccg ttcttcgagt
    25741 ccagcgtgct atcacgcggg ctgattgcgg aacaccaggg cgagctgtgg gtcgccagcc
    25801 cgctgaaaaa acagcgtttt cgcttaagtg atggcttgtg catggaagac gaacagtttt
    25861 ccgtcaaaca ttacgaagca cgagtgaaag acggcgtggt gcagctgcgc ggttaatgtt
    25921 ttaacgggag gcgcaatgcc tccccttttt gcatggtcct gtaatgacct tcggtatatt
    25981 gcaggacatt ttttaaactt tttgttttat tttttgtttt tattttttaa aggataatca
    26041 aatgtttaca gacactatta ataagtgtgc ggctaatgct gcgcgcattg cacgcctgtc
    26101 ggcaaataac ccgctcggct tttgggtcag ctccgccatg gcgggcgcat atgtgggtct
    26161 tgggatcatc ctgattttca cgctcggtaa tttgctcgac ccgtccgtac gtccgctggt
    26221 gatgggcgcg acctttggta tcgccttaac gctggtgatt atcgccggtt ctgaactgtt
    26281 caccggacac accatgttcc tcacctttgg ggtaaaagcg ggcagcatca gccacgggca
    26341 aatgtgggca atcctgccgc aaacctggct gggtaacctg gtcggttccg tcttcgttgc
    26401 catgctctat agctggggcg gcggtagcct gctgccggta gataccagca tcgttcactc
    26461 cgtcgcgctg gctaaaacca ctgcaccggc aatggtactg ttcttcaaag gtgcattgtg
    26521 taactggctg gtttgcctgg caatctggat ggcgctgcgc actgaagggg cggcgaaatt
    26581 tatcgctatc tggtggtgtc tgctggcatt tatcgcgtcc ggctacgagc actctatcgc
    26641 taacatgacg ctgttcgcgc tctcctggtt cggcaaccac agcgaagcct acacgctggc
    26701 gggtattggt cataacctgc tgtgggtgac gctgggtaat actttatcag gtgccgtatt
    26761 catgggattg ggttattggt atgctacgcc gaaagcgaat cgtccggttg cggacaaatt
    26821 taatcaaact gaaacggctg ccggttaatt actaaggggt ttttacgtgg atcatttgcc
    26881 tatattttgc caattacgcg atcgcgactg tctgattgtc ggcggtggtg atgtcgcgga
    26941 acgcaaagca aggttgctgt tagacgcagg cgctcgctta acggtgaatg cattagcgtt
    27001 tattccacag ttcaccgcat gggcagatgc aggcatgtta accctcgtcg aagggccatt
    27061 tgatgaaagc cttctcgaca cctgctggct ggcgattgca gcgacggatg atgacacgct
    27121 taaccagcgc gtcagcgaag ccgctgaagc tcgtcgcatc ttctgtaacg tagtcgatgc
    27181 gccgaaagcc gccagcttta ttatgccgtc gattattgac cgctcaccgc taatggtagc
    27241 ggtctcctct ggcggcacct ctccggttct ggcgcgcctg ttgcgcgaaa aactcgaatc
    27301 actgctgccg ttgcatctgg gccaggtggc gaaatatgcc gggcaattac gcgggcgagt
    27361 gaaacaacag ttcgccacga tgagtgagcg tcgccgtttc tgggagaaat tgttcgttaa
    27421 cgaccgcctg gcgcagtcgc tggcaaacaa cgatcagaaa gccattactg aaacgaccga
    27481 acagttaatc aacgaaccgc tcgaccatcg cggtgaagtg gtgctggttg gtgcaggtcc
    27541 gggcgatgcc gggctgctga cgctgaaagg actgcaacaa attcagcagg cagatgtggt
    27601 ggtctacgac cgtctggttt ctgacgatat tatgaatctg gtacgccgcg atgctgatcg
    27661 cgttttcgtc ggcaaacgcg cgggatacca ctgtgtaccg caggaagaga ttaaccagat
    27721 cctgctgcgg gaagcgcaaa aaggcaaacg cgtggtgcgg ctgaaaggtg gcgatccgtt
    27781 tatttttggc cgtggtggcg aagagctgga aacactgtgc aacgcgggta ttccgttctc
    27841 ggtggttccg ggtattaccg cagcttctgg ttgctctgcc tattcgggta ttccactcac
    27901 gcatcgcgat tatgcccaga gcgtacgctt aattaccgga cacttaaaaa ccggtggcga
    27961 gctggactgg gaaaacctgg cggcagaaaa acagacgctg gtgttctata tggggttgaa
    28021 tcaggccgcg actattcagc aaaagctgat tgaacacggt atgcctggcg aaatgccggt
    28081 ggcaattgtc gaaaacggaa cggcagtcac gcagcgcgtg attgacggta cgctcacgca
    28141 actcggtgaa cttgctcagc aaatgaacag tccatcgcta attattattg gtcgggttgt
    28201 tggcctgcgc gataaactga actggttctc caatcattaa tttaacccgg gccagagaat
    28261 tctggccttc ttaatgactc tttttatata ttcagcaaat aaaacataac cccttaataa
    28321 atattatctg ctaatgggtt atattcaccg ttacagcgac ttacaaaatt aagtcattcg
    28381 ctcactgacg ccaattaaat attcctgtat cgttcttaac gagcagtctg cttatattca
    28441 tataatcaat gaatattaat taatatataa tacataggga atgtaaatga acaaatttat
    28501 taaagttgca ctggtaggtg cagtactggc tacgttaact gcatgtactg gtcatattga
    28561 aaaccgtgat aagaactgct cttacgacta cctgctgcac ccggcaattt ctatttctaa
    28621 aatcattggc ggttgcggtc ctactgcaca gtaagtctct gacagaaatc ggctaacacc
    28681 ggtttaattg cccgacgccc tctggatttt ccgagggcgt atttgtttct atgtccttct
    28741 tgcccccgcc cgtaaaaata ttttcacttt aaattcaatt ttataactac atccattagc
    28801 cacagcgcga tcctgtgcga aattttgtga tcttcctcca cattacacaa catcatatgt
    28861 tgttatattc atcatgcatt gtcatgttgc cttttaaatg actgcaaact ctcccctaca
    28921 acgtattgga caagaaaaag gtatcgctat gggaagccag gaactccaac gcaagctcgg
    28981 attttgggcc gttcttgcaa tcgccgtcgg gacaaccgtc ggctccggta tttttgtatc
    29041 tgtgggtgaa gtggcaaaag cagcgggcac gccgtggctt acggtgctcg cgtttgtcat
    29101 tggcgggtta attgtgatcc cgcaaatgtg cgtctatgcg gaactatcca ccgcttatcc
    29161 ggaaaatggc gcagattatg tttatctgaa aaatgccgga agccgaccgc tggctttcct
    29221 ctccggctgg gccagcttct gggccaacga tgcgccgtca ttgtcgatta tggcgctggc
    29281 gattgtcagc aatcttggct ttttaacgcc tatcgatccg ttgctcggta aatttatcgc
    29341 cgccggatta attatcgcct ttatgttgct acacctgcgc tccgttgaag gcggcgcagc
    29401 gtttcagacg ctaattacca tcgccaaaat tatcccgttc actatcgtca ttggccttgg
    29461 gatcttctgg tttaaagcgg agaattttgc cgcccttacc accactgcga ttggcgcaac
    29521 gggcagcttt atggcgctgc tggcggggat ctctgccacc agttggtcgt ataccggcat
    29581 ggcctctatc tgttatatga ccggcgaaat taaaaacccc ggaaaaacca tgccacgagc
    29641 gctgattggt tcctgtctgc tggttctggt gctctacacc ctgctggcgc tggtgatttc
    29701 cggcctgatg cccttcgaca aactcgccaa ttctgaaacg ccgatttccg acgccctgac
    29761 ctagatcccc gcactcggca gcaccgctgg gatctttgtt gccatcacgg cgatgatcgt
    29821 cattcttggt tcgctttcca gctgcgtgat gtaccagccg cggctggaat acgcgatggc
    29881 gaaagacaac ctgttcttta aatgcttcgg ccatgtgcat ccgaaataca acacgccgga
    29941 tgtctccatc atcctgcaag gggcgctggg gatcttcttc atcttcgttt ccgatctcac
    30001 cagcctgctg ggttatttca ccctggtgat gtgtttcaaa aataccctca ccttcggctc
    30061 catcatctgg tgtcgtaaac gcgacgatta caaaccgctg tggcgtactc cggctttcgg
    30121 gctgatgacc accctcgcca ttgcgtcaag cctcattctg gtcgcctcaa cctttgtctg
    30181 ggcaccgatt cccggcctta tctgcgccgt catcgttatt gctactggtc tgcctgctta
    30241 cgccttctgg gcgaagcgta gccgccagct caacgctttg tcgtaatgtt atctgaagaa
    30301 aataaaatgt tggatattga taaaagcacc gtggactttc tggtcaccga aaatatggtt
    30361 caggaagtgg aaaaagttct cagccatgac gttccgctgg tgcacgccat cgtggaagag
    30421 atggtgaagc gcgacattga tcgtatttat ttcgttgcct gcggctcgcc actcaacgcg
    30481 gcgcaaacgg cgaaacatct ggcggatcgc ttttccgatc ttcaggtcta cgccatttcc
    30541 ggctgggagt tctgcgataa caccccgtat cgcctcgacg atcgttgcgc ggtgattggc
    30601 gtttctgact acggtaaaac ggaagaggta atcaaagcgc tggagctggg ccgggcctgc
    30661 ggcgcactca ctgcggcgtt caccaaacgc gcggacagcc cgattacctc ggcggcggaa
    30721 tttagcattg attatcaggc cgactgtatc tgggaaattc acctgctgct ctgctacagc
    30781 gtggtgctgg agatgatcac cctcctcgcg ccgaacgcgg aaatcggcaa gatcaaaaac
    30841 gatctcaagc agttgccgaa tgcgctcggt catctggtac gcacctggga agaaaaaggc
    30901 cgccagcttg gtgaactggc cagccagtgg ccgatgattt ataccgttgc tgcgggtccg
    30961 ctgcgtccgc tgggttacaa agaaggcatt gtaacgctga tggaatttac ctggacgcac
    31021 ggctgcgtga ttgagagcgg agagttccgc catggcccgc tggagattgt cgaaccgggc
    31081 gttccgttcc tgttcctgct cggcaatgat gaaagtcgcc acaccaccga acgcgccatt
    31141 aactttgtta aacagcgtac tgacaacgtg atcgtcatcg attacgccga aatttcgcaa
    31201 gggctgcacc cgtggctggc accgttcctg atgttcgtgc caatggagtg gctctgctac
    31261 tacctgtcta tttacaaaga tcacaacccg gatgaacgcc gctattacgg tggtctggtg
    31321 gaatattaat ccctctcccg gcccggcaac gggccggatt tacgcaagga gttacccgat
    31381 gaaaacaggt atgtttacct gcggccacca gcggctgcct attgaacacg catttcgtga
    31441 tgcaagcgag ctgggttacg acggcatcga aatttggggc ggtcgcccgc acgcgttcgc
    31501 gccggactta aaagcgggcg gcatcaaaca aatcaaggcg ctggcgcaga cgtatcagat
    31561 gccgattatc ggttacacgc cagaaaccaa cggctatccg tataacatga tgctgggcga
    31621 tgaacatatg cgtcgtgaaa gcctggacat gatcaagctg gcgatatata tggcaaaaga
    31681 gatgaacgcg ggttatacgc tgatttctgc ggcccacgcg ggctatctca cgccgcctaa
    31741 cgttatctgg gggcggctgg cagagaactt aagcgaactg tgtgagtacg cggaaaacat
    31801 cggcatggat ctgatcctcg aaccgttaac gccgtatgaa tcgaacgtcg tgtgtaatgc
    31861 taatgatgtg cttcatgcgc tggcgctggt gccttcgccg cgcctgttca gcatggtcga
    31921 catttgcgcg ccgtatgtcc aggcggaacc ggtgatgagt tatttcgaca aactgggcga
    31981 taaattacgt catctgcata ttgtcgacag cgacggggcc agcgacacgc attacattcc
    32041 tggagaaggc aaaatgccgc tgcgggaact gatgcgcgat attattgatc ggggctatga
    32101 gggttactgt acggtggagc tggtgacgat gtatatgaac gagcccagac tctatgcccg
    32161 ccaggcgctg gaacgctttc gcgcgctgct gccggaggat gagagatgaa aaccctagcg
    32221 acaatcggcg ataactgcgt cgatatctac ccgcaactga ataaagcgtt ttctggcggt
    32281 aatgcggtca atgtggcggt gtactgcact cgctacggca tacagccggg atgcattacc
    32341 tgggtgggtg acgatgacta cggcacaaag ctgaagcagg atctcgcccg catgggcgtc
    32401 gatatcagcc atgtccatac gaaacacggc gttaccgcac aaactcaggt ggaactgcac
    32461 gacaatgatc gcgtttttgg cgactacacc gaaggcgtga tggccgactt tgccctgagt
    32521 gaagaggatt acgcctggct ggcgcagtat gacattgtgc acgcggcaat ctggggacat
    32581 gcggaagacg cattcccaca gctgcacgct gcgggcaaac ttaccgcttt cgacttctcc
    32641 gacaagtggg acagcccgct ctggcagaca ctggtgccgc atctcgattt tgcctttgcc
    32701 tccgcaccgc aagaagacga agcgctgcgt ctgaagatga aagcgattgt tgcccgtggc
    32761 gcaggaacag tgattgtcac gctgggtgaa aacggcagca ttgcctggga tggcgcgcag
    32821 ttctggcgtc aggctcctga accggtgacg gttatcgaca ccatgggtgc cggagattcg
    32881 ttcattgccg gattcctttg cggctggtct gcggggatga cattaccgca ggcgatggcg
    32941 cagggaacgg cgtgcgcggc gaaaaccatt cagtaccacg gtgcctggta ggtataacgt
    33001 tggcgtgagc atcttcacgc caacgtgctg ttacttgccg gaaaacgacc ctataatccg
    33061 agtaattcat tctttatttc agggtcgatt atgtcagcta cggaccgcta ctctcatcaa
    33121 ctcctctacg ctaccgtccg ccagcggctg ctggatgata tcgcgcaggg ggtttaccag
    33181 gccgggcaac agatccctac cgaaaacgag ctttgtacac aatataacgt cagccgcatt
    33241 accattcgca aagccatcag cgacttagtg gcagacggcg tactgatccg ctggcaggga
    33301 aaaggcacct ttgtacaaag ccagaaagtt gaaaacgccc tgcttactgt cagtggtttt
    33361 accgattttg gcgtctcaca aggcaaggcg acgaaagaga aagtgatcga acaggaacgg
    33421 gtcagcgccg cgccgttttg cgaaaagctg aacatccccg gaaacagcga agtgttccat
    33481 ctctgccggg tgatgtatct cgataaagag ccgctgttta ttgatagttc atggatcccg
    33541 ctgtcgcgtt atcctgactt tgatgagatt tacgtcgaag gaagctccac ctatcagtta
    33601 tttcaggagc gttttgacac gcgagtggtc agcgacaaaa agaccatcga tatctttgcc
    33661 gccacccgcc cgcaggcaaa atggctgaaa tgcgaactgg gcgaaccgtt gtttcgcatc
    33721 agcaaaatcg cctttgatca gaatgacaaa ccggtgcacg tctccgaact cttctgccgc
    33781 gccaatcgca tcaccttaac gattgataat aaaagacatt aaccgtaggc cggataagat
    33841 gcgccagcat cgcatccggc gatgctggcg cactgaattt tacatcccgc acgttcccct
    33901 caccctaacc ctctccccaa aggggcgagg ggagcgttca gtgcggaatt tagcttgatt
    33961 gccgaacggt gctatccagc attaaatact cgcaatactc tcacgcaaaa tccgcagcac
    34021 cgtctcttca ccgctgcgat tcgggttaat gcgaatcgca caatgttcga gttgtggatt
    34081 cgcctggcga aacgttcctg aaagtcgata gaagagcggc gggatttcat atttcgactc
    34141 tgcaccgacc gggtaaggca acgcgccacg cttttgcgct tcttccagca ccctggcggc
    34201 aatcagctga tgaaactcga caatcaacac cttcgactgc gcattggcaa tgacggcgct
    34261 tttcacttcc gccaccgcgc cgccgttaag caaagccagc aaccgttcgg atactcccgc
    34321 ctgcaccgca tgcatcactg gcgcgaacac cagaccacgc aagacttcca gcgcctgtga
    34381 gccctggatc tggctaccgc cggagtaaag cgtggcgcga atccgactaa taacatcggc
    34441 atcgccaacc actgcaccaa cgccctccgg cccaaacagc ttgaagcagg agaatgtcga
    34501 gacattcgcg ccgcattcac agccgattcg cgctaccttc atcaccgcat agttatcatc
    34561 ggttaacgct ggaacacctg ccgcgcgcaa cgttgccagc acatctgcca gcacatagct
    34621 gtcctgcggc tgctggcgcg tatgctgcac cagcgcggca tcaggttgtt gctcgtcgac
    34681 gacctgcttc agtgccaaca ggtcattgaa atcagcagta ataagcgtca gccccatctg
    34741 ctcaataata acccgtgtcg tcgggtaaac aggcgcgtca tgcaccagaa gacgctgccc
    34801 cggtttgagc aaagccgcca gcccggcgcg aatcgcgcca gtccccgcgc cctgcaccag
    34861 cgccgcggcc tgtgcgtgaa atgcatcagc cagcacctgc tccacccgtt gggtaatacg
    34921 cggttgattc agacctggag ttaaccctaa atcaccgcca gcaagaaact cgctgcctgg
    34981 aaaatggcgg caaatagtgt ccaccagcgc aaactgcttt tgctgcgctt cagcaagagt
    35041 caggctttgc aaaggaaacg tcttcatagg agccttcctt aagccggaac gaacaggcca
    35101 agccagtaaa gaacattcag cagaataccg gtgatcatta ctgccacgac cggtgccgcc
    35161 attttctgta ccggacggcc cagggattcg ttgaggaagt aaatagcaac cgcgatagag
    35221 aatccggtat aacccgccat cttaattgct gcgaaaatcg aaccgaccag cagcgccatt
    35281 tccatcagca tattcatggc gttacggatg ttatccgacg cattacgcac cgacgggtag
    35341 cgtcccagcc atttgccgat cgaacgaagc agcaagactt ccgccgaaat aaccactgcg
    35401 cctaataccg ctgcaaccat cggattcggc gagagatagc ccaccgcata aacaaaggta
    35461 aagcccgcaa ctgcatacac acccgttgct aaggcggtgg tggcaatcaa cggcacaaac
    35521 cccagtccgc gcataaattc tgccagagcc gcctgattaa tcagcgtttg cgattgttcc
    35581 ggcgtcacgc ctgcggaata cgctttctcc agtgtgaaga tcgaaacttc gctaccagca
    35641 aaaatcttca tactggcaac ggcggcaatc aatgctccca cgatggcgat ataaggtaag
    35701 tttttgataa tccgcgacgt gcgctcttca aacaccgaaa gcccgctggc atcgatgtca
    35761 ttttcatcac gatgacgcag gtcgtgagtt atcgcgatcc ccagcagcat caccatgcca
    35821 ataaagattt cgatggattc agggttaaga tgtgggaaat agcgcacgac taccacacgg
    35881 gtcatcagaa caaccacggc ggcgaccaga ctttgcttcc agccaaactg gtagaaaatc
    35941 gccaccagcg ggaacagtgc aaaagcagaa accaccggcg agcttaattc cccgaggcta
    36001 cctaatacat ccaccggcag cgcggtcagc agctggttaa ctggcaacag gcaagtaagg
    36061 atcaacacgc cccagatagc gccaagacca aacgccatca ggctgtttat cgccagtacg
    36121 ccgaggatat cggtaggaag aaagagtaac catgcgttga gcagccaggt tttcagggta
    36181 aaagagatcc ccaccgaggc cacaaaacca atgctcagac caaaagcgat actccccgcc
    36241 tcgcgacggt tcatatagcc ttcaatcagt tgcggcagga tcgggtggat gccgtcatga
    36301 aaaacagccg ccgagcgatg cgccagaagc gatgtcatac ccgtcaggca cgccaccacg
    36361 ataatctgaa tatacagatc catagtcgtg ccttttattt cagatggtta atcaacatcg
    36421 gaatcgcgtg ttcgacgtgc tcaacggaaa ggccaaacgc cactttccct tcagcgatca
    36481 ttttggcgat atgctcgtct ttcgctttat gccgcgcccg caccggtatt acacgcgcca
    36541 atgtaataat ctagttgccc ggatttcacc ttcattgccg cttccatgtc gttgtgaatg
    36601 aaaacttcaa aacagccagg agccgtcgct tcaatggttt ttttaatctg ctcacgctgt
    36661 aagcctgcaa cgccaatctt tttcatgagt ctgtccttat tggaaaaatt gagagggatt
    36721 ttcacgtaac atcacatcca catcggcctg actgaatcct gactggcaca attgcggaat
    36781 aaaggtggtc aataaatagt catagccata accaccattg gcttttaaat gggagcggcg
    36841 cgtaatatcc atcgacagca tgacgcggtt cagcaaccca cggtcacgta gcgcgtggag
    36901 catcgcaatg cgcttttcgt ccgggtagta actgttctta ccaatggtgt cgaactgcac
    36961 gtacgcgccg agatcgatca tcttcaaaat gttgtcgagg ttgtctttca gatcgcagtg
    37021 accaacggtg acgcgcgaaa gatcaactcc gtgggcttgc aacagagcca gttgttccag
    37081 ccccatggtg ctgaacgacg tatgcgtgga gatcgggcgt ccggtctggt tatgcgccag
    37141 cgcagcggca ataaatacct tctcttccag cggcgtaatc tttccttcgc tggagccgat
    37201 ctccgcgatg atcccggctt tcagctccgt gccatcgata ccctgttcaa tttcatcgac
    37261 catctcctgc gccagttcct gcacgctgcg ggtcaccaca tgttccggga aaaacgcatc
    37321 ctggtaataa ccagtacagg ccaccacgtt gatcccggtt tcgcgcatta catcgagcat
    37381 aaattgcgca ttgcgcccca tgtaacggtt ggtcatctca atcacattac gtacaccccg
    37441 ggtcatcagg tcgttcatct cctggcaaat gaacgcatac tgatcaaggc ggcagtccac
    37501 gttgttttta aagccggaga gatcaatatg cagatgctca tgggccaggg tgtaacccgt
    37561 cggatcaaaa ctcatagggt gtctcctgca aaccggaggg aggaaagaaa agagcgacca
    37621 ttttgtggcg gtggcgcacg gaaaaattca cacacggtag ctcccacatc agaaagcgtg
    37681 gtgcgcacac cgagctgcgt ggcgaccagc ccttgctgat aaaccagcac tggcaccact
    37741 tcacgggtat ggtggctgtg accaatggtc ggatcgttac cgtgatccgc catcacgacc
    37801 aggcaatcat ctggctgcat cgcctcaaca agccgggcaa ggttacggtc aacgacctgc
    37861 aaacgttcgg cataacgtgc gacgtcttct gcatgaccag cgaggtcggt ttcctggata
    37921 ttgatgcaaa taaacgccgt cggatgggtg ttaaattcgt cgagggtgat atccataatc
    37981 cgctggctat ccaccaggtt ttgccagctc acaccataag gattgctgac gatatctgcc
    38041 accttaccca ccagcacggt tggcacgccc gcttcataca gtttttgcgg cacctgcact
    38101 ttttcatcaa caccgtagcc catatgcacg acctggaagc cgttgtcata agcgccagaa
    38161 cgcggcgcat taataccaat aaagcgccct tctttgcttt ctgcggcatc gagaatacgc
    38221 tgactgttgg ttaacaggcc gccaaatgta atgacccgac cgacctgtac ctggtcacgc
    38281 acgatacgac caattttgat tgcgtcgtca aaagagatca cagagagatt ggcggtaatg
    38341 ttatagactt ggcctaaatc cgcctcgaga ttatcgccaa tcgcaaccgc ctgattgacc
    38401 cacagaaatt gcagatcatc gccacggcgc tccacctgcc agccagcgga aactaatgcc
    38461 tgctcaacac ggtcaatcac atcgcgaaaa ggcatccgca gcggcggtaa cgggcgcgtg
    38521 cctaaaattt cctgatgccc cataaaggta tcgccacctt catgttgcag ctctgccacg
    38581 ccccaggttg cggaatctga cggctgcata tcgcctggcg cataacccaa tgtgttgatt
    38641 agccccagct tctccaacgt tggtagctgc aaatgcggca actggctcag gatgtgacca
    38701 caggtattcg ccccggcatc ttgcggacgc accagcgtga catctttcat tgcccctacg
    38761 ccaaagctat caatcactaa caccacaaat cgcgccattt atgccttcag agggttgccg
    38821 aggctgtcat aacgaccaac tatttctggt tcgccgcgct gaataccgga caccagcacc
    38881 acatcgctac gggtgacaaa aatctgcgtg cgaaaacaga gcaccactgc actgctcacc
    38941 ggatactcgc ctgccagcgg cagggtatag tcgatactgc tgtcatccac ggttttgaga
    39001 ttggtttcag taatcttttg attttctggc gtaaacacca gcgcatgttg cgcatgacca
    39061 cgacggtagt aaccgccgcc gtagcagtag ctgtcgccac ggaaatgatg ggagatttca
    39121 cttaaccaga gcatcgcgat acgttcaggc tgatcgccct gctggtttgc cggaatagta
    39181 ccggtcagcg catgaccggg ttcggcatga gtcgcaccgt attccgccag caatggcagc
    39241 gaagtgcagc tggtcgctga aggtacgttc agttgctcaa tcgcaatacc agatttcgcc
    39301 agttgatccc gtgcttgtac cagcgtgtga agattcggtg ttggcaaaac tttcccggca
    39361 gcctcatccc aaagcaggca agggaaatgg gttagtccgg ctaaatgcag ccccggcagt
    39421 ttcttaattt cagcaatcac ctcgtgcagc gaatgctgta caaaaccgct ctcctgcccc
    39481 ggataaagaa aatcatcgtc gctataaact ttgagcagca caggctggac ccgtccggcc
    39541 ttaaccgctg ccgcagaaac ttcccgtgct ttgtcgagag taaacacggt gatgatgtcg
    39601 gtgccctgtt caacggcgtc agcaacctga tgacaaggga tttgtaccag atgcccctga
    39661 tgcgccactg gcaaaccagc gcggcgcatg actcgcgcct ctttgtaatc caccgccaca
    39721 atgccgctgt agcctaatgc cagcaatttt tccgccagcc acggattacg accaaattgc
    39781 ttggtcatca gatacagttc aatgccgtaa agccgcgccg tctcaatgag ccgcttaccg
    39841 ttttccagta tctgatccac gtcgatcacc cagctgtccg gggcgatttt gccctgctgc
    39901 cacaaactta gtgcggcgga aatcagcgcc gggttctggc gtttcaatgc ttctacaaac
    39961 atcttgttgc ctcgtttctg ttccttgaat taaatattca tttttttaaa tatttaggtt
    40021 gtgcgacgtg gatttgtcgg gagcttcaaa cctcttcgtt agcagccatc cataatccat
    40081 aaagattggc cagaaaatag ccttcttcac acgggttaac ttccagagcg aattccttca
    40141 acaacacctg atgcaattgc acaacggctt gccagtggct ggattgcgcc agttccgcca
    40201 gcaactcgtt atccagcggc tctatttctt caccacggcg actgcgcatc agtgcactcg
    40261 ccatatgtgt catcgccatc gttccttgct cactgcgcac cggcagatgg cactcttttt
    40321 ccagtacgtt gacgacctgc atcatgcctt tgcagacgtc cttatcaata acgcctgcct
    40381 cgcaaagcag gttgagtctg gtttccatat cagatgcctt tcgtttaata actgggttcc
    40441 tgttccccac tcaccacccg ttgttgatgg gcaagcaggg cgtgtttatc tacaacggca
    40501 cgcagcaatt gttgcattgg gtaatcatcg actcgcgtca ggaccacggc ttcggtggcc
    40561 agtaaaaatc gcggatcgtc tgttagcggc gtcgcctcta atcccagcat ggtcagttcg
    40621 ttttccgcca ccacgttcca gatcaccgca tcgacatcgc ctttaacaat gcgttgtaaa
    40681 ctctcgtgat aagagagatc gactcgttcc acatcactat cgccaaaaaa gacatcggtc
    40741 atgattttct gatccgccga acgactatcg agacctactc gcttaacatt tccagactca
    40801 cctttgcggc atatcagttg atgttcacca acatacgtat gcgggccaag ctcgagggcg
    40861 atgcacaaat tgttttggct gagataactt tccgccgcca ggcgggaaac tactgccatg
    40921 tcatagacac cgttaagtaa acactccacg cgaatatccg cgccgcgcat atgcgcatag
    40981 taaaaaggaa tgccatcaaa ctgggctttc aatccgctcg ctaaaccttc gtacaagcgg
    41041 gtatagggca aaggcatcgc acataccacg ttgttgatat ccacatgact cagcaatgct
    41101 ttgttatcca tctcgacaag ataactgcca ttgcgcccac ggcgttcaat ccgtatcgct
    41161 ccgcttgatt ccagcgtttt caacgcggcc tgcgtcaggc caaccgatga acggcattca
    41221 gttgccagct catctatggt tttcagtcga ttaccgcatt tttcacccaa caaataacgg
    41281 gccagtgtcg tgatgacgac gccttctttt ttgataaacg ttcgacgcat aatatatttt
    41341 caataaaatg aatatttata tcttcattaa aatgaagata agtggcgacg acgagatgtg
    41401 gaaacggcga ggcgcttcac attttttcgg gttgttgcga aaagaggata aaaaaaccgg
    41461 gtttccccgg tttcagagtg atgacgaagt gatttattcg acaatgcgag caaagggtaa
    41521 ttcccactgg cgcttgcctg ctcccctcac cctaaccctc tccccaaagg ggcgagggga
    41581 ccgtccgagc gttttgatat tacggcttcg ccacaaaacc aatcgcttcg tacaccgctt
    41641 tcagcgtacg ggaagcgtgt acactggctt tttctgcgcc gtctttcatc acctgttgca
    41701 ggaaggcttc atcgttgcgg aaacggtgat agcgttcctg caattcagtc agcataccgg
    41761 aaacggcatc agccacttca cctttcagat gaccatacat cttgccttcg aactgttttt
    41821 ccagttctgg gatgctctgg cccgttaccg cagaaaggat atccagcagg ttggaaacgc
    41881 ccgctttgtt ctgcacatcg tagcgaacta ccggcggctc gtcggagtca gtgaccgcgc
    41941 gtttgatttt cttcactacc gatttcggat cttccagcag gccgataacg ttattgcggt
    42001 tatcgtcaga cttggacatc ttcttggtcg gctccagcag cgacattacg cgcgcgccag
    42061 atttcggaat aaacggctcc ggcaccttaa agatctcgcc atacagcgcg ttgaaacgct
    42121 gggcgatatc gcgactcagt tccaggtgct gtttctggtc ttcacccacc ggtaccagat
    42181 tagtttgata cagcaggatg tccgcagcca tcagcaccgg atagtcaaac agaccagcgt
    42241 tgatgttctc ggcataacgc gcagatttat ctttaaactg cgtcatgcga ctcagttcgc
    42301 cgaagtaggt atagcagttc agtgcccagc ctaactgcgc atgttctggt acgtgggact
    42361 gaacaaaaat ggtgcttttc tcaggatcga taccacaagc cagatacaag gccagcgtat
    42421 ccaacgtcgc tttacgcagc ttctgtgcat cctggcgcac ggtgatcgcg tgttggtcaa
    42481 cgatacagta aatgcaatgg tagtcatcct gcatgtttac ccactgacgc agcgcaccca
    42541 tgtagttacc aatggtcaat tcacctgagg gctgtgcgcc actaaaaacg atgggcttag
    42601 tcatttttcg attcctgatt ttcgctatgc ggaagcccta atgcgggcag aaggtcattt
    42661 atagactgat aaattacatc aggctggctg agatcgatag cctcgccgta gttatatccg
    42721 taggttaagc caactgatgg gcaacctgcc gcttttgccg cctgaatatc attgcgtgag
    42781 tcgccgacaa acagcatctg ttgtggggca attcccatcc gctcagccac cagtaacagc
    42841 gggtccggat gcggtttttt gttttgcaca tcatcgccgc cgataaccac gctaaagtat
    42901 ttggcaatat ctaaggcttc gagcagcggc gcgacgaacg gcgtcggttt gttggtgacc
    42961 aggcctagcg gcaggccttt agcctgcaac gcgcccaacg tatcggcaac gtgcgggaac
    43021 aaaaacgtcc cctcttcggc aacctcgcca tagtagcgat cgaacagttt acgcagaata
    43081 cgtacctgtt cttctgccgg aatgtcgtca tcaacgggcg gtttccccat tgttttacgc
    43141 agagtcgcac gttcctgacg cgcccaggtc aatgcgcgct ccatcagaac atctgcgccg
    43201 ttaccaatcc aggtaataac gcgttcttca cctgcgacgg gcaactccag cgcatacagc
    43261 gccatatcta ccgcagcagc aagaccagga gcactgtcga ccagcgtacc atcaagatca
    43321 aaagcgacgc cgcgaatatc ttcaaactta ttcatgactt acctttgcca gttcactgcg
    43381 catttcatca atgacttttt tatagtctgg ctggtcgaag attgccgaac cggcgacgaa
    43441 catatccgcg cccgccgcag cgatttcgcc aatgttgttc accttcacgc caccgtccac
    43501 ttccagacga atgtcaaagc cagactcgtc gatacggcga cgtacttcgc gcagtttatc
    43561 cagtgtttga ggaatgaaag actgaccgcc gaaaccaggg ttgacggaca tcagcaggat
    43621 cacatccagc ttatccatca cgtaatccag atagctcaga ggtgtcgccg ggttaaatac
    43681 cagacccgct ttacagccat tttctttaat cagttgcagc gtgcggtcaa catgctcgga
    43741 ggcttctgga tgaaaggtaa tgatgctggc accagcggca gcgaaatcag gcacaatgcg
    43801 atcgacgggt ttcaccatca gatgtacgtc gataggggcg gtaatgccgt agttacgcaa
    43861 ggatttcagc accattggcc caatcgtcag attgggaaca tagtggttat ccatgacgtc
    43921 aaaatgcacg acatcagcgc cagctgccag ggcttttgcg gtatcttcac ccaggcgggc
    43981 aaaatcagcc gacagaattg agggggcaat caaatactgt ttcatccgct tctccttgag
    44041 aattattttt tcgcgggtga aacgactcct ggtttgtaca aagccagcag ttcgtccacc
    44101 tttttacgtg tgccgccgtt gctgcttata ctgcgtcgaa ctttgacgac atgcaatttt
    44161 gcgcgctgat accactcacg cgttaacatc gtatcgtgat tggagatcag cactggaata
    44221 tggcgctcaa ccagaccttc ggcgatctcc gccagatgcg cttgttgttc aagcgtaaaa
    44281 ctgtttgtgt gatacgccgt aaagttggcg gtcgcagaca gcggtgcata aggcggatcg
    44341 caatagacga cggatgcatc atctgcgcgc tccatgctat cggcgtaaga ctcacaatag
    44401 aaaaaggcat tctgcgcttt ttcagcgaag tgatacaact ctgcttccgg gaaatagggt
    44461 tttttgtagc ggccaaacgg cacgttaaac tcaccgcgca gattgtaacg acacaggccg
    44521 ttgtaaccgt agcggttcaa atataaaaac agtaccgccc gacggaacgg atcctggctt
    44581 ttgttgaact cttcgcggaa ctgatagtaa acctcggcgc aatttgtttc gggaacaaac
    44641 agctcgcgtg cggcctgtac gtactcatca gtacgcatct tcacaatgtt atagagactg
    44701 atcaggtcgc tattgatatc ggcaaggata taacgagaaa agtcggtgtt gagaaacacc
    44761 gacccggcac ctacaaaagg ctcaaccaga cattcgccct tgggcaaatg ccgtttaata
    44821 tcatcaagca ggggatactt gccccctgcc cacttcaaaa aagcgcgatt tttcttcatg
    44881 ccgactaact aattacacct tctccggctg tggagaaagc tccgacagca tccagcgctt
    44941 caggcagctc ccgcaacgat aaaccattgc gggagataac cttgattact tcagatcggc
    45001 ctgtacctga cgcagcggtt tcgcccacgg gtttttggcc tgaacatctg ctggcaatgt
    45061 agataccgct tttttcgcct cttctttcga agcgtacacg ccagaaacca ggacatacca
    45121 cggctgacca ttacgcgtcg tttcatagac aacgtagttt ttcagattct ctttcttcgc
    45181 ccaaccgttc aggttgtcgt agttagagga actgctcagc tgcagagtgt aatggctgga
    45241 cggtgccgat ttcaacgaac caacattacc tgcggtcttc cctccagcgg ctggtgttgc
    45301 cgtggtttgc gccggggatg ccgtctgtac tggagccgta gtcgccgtct cttttggtgc
    45361 tggcgtagaa gtcgcagccg gtgccttcgt gctcgccaca ggggcagctg gttcagtacg
    45421 cttcggcgtc tgtgctaccg gcttcggctc cgttttcacg gttgcttgcg gttttttcgg
    45481 ttcaatcacc gcctgctgac gaactgggcg cgtagtggac ggacgttcag cggtttgcgt
    45541 tttcgccgtg tcacgcgatg cattgccatt gcgaacaggc gcaaccgttg cgggttcagt
    45601 cggcaacgtg gaattgaccg ccacattgtt cagctgttgc tgattttgtg gctgggtcag
    45661 cgcattgttc aggtcaccct gcacttcaac acgttgttga ccatccgttg ccaccggggt
    45721 ttgcccttga gtcggcgtag aagagatcgg tggcagagaa acatcctgct gagtattttc
    45781 cgcagacgtg gttcccggcg ctggctgcac gccattcgcc tgatcggtcg cattgccagc
    45841 aagatcaata ctcttctcgc cagacgcggt ttgatcgctg gaagtggtcg agggggcttt
    45901 tagcgcagaa ccgataccga tgatcaacag cagtagaacc agaatgccga cgcccatcat
    45961 catatactga cgagaagcgg gtttactggc tgcttttttg cgcttacgcg gacgacgctc
    46021 tacgcgctct tcatccacgg tttcatcttc ggattcgtca atttcttctt cgatttccgg
    46081 ttcctcattg cgctcttttt gcgcacgagt cggacggcga tcgtcagtgt catcaagttc
    46141 aatatcatca aaattgatct gcggttcgcc acgttcagta cgctcagaac gttcagaaga
    46201 ttgacgagaa cgaccagtac gacgatcgct gggatcgggt ttcagctcgt cttctggttt
    46261 gaattcatcc atttaacacc ccactaaaag gttaatgctt accacgttgc aattaacctg
    46321 aagctaatag accgcttgat aagcggcctg acctttcttg ttgttacgct gattgacaat
    46381 cggcaatggc gttaagaaca agctcgtgcg aaacgccgct gcgaacttca ctcttaccaa
    46441 ttgccaacgg aagaattaag cgcatctctc ccgcaaggac tttcttgtca cgcagcatat
    46501 gcggtaaata cgcctgcgcg gacatttcgc gcggcccatt gaccggtaac ccagcccgcg
    46561 tgagcagggt tataatacgc tgcgtttcgg cagaactaaa ctgcccgaga cgttccgacg
    46621 tccgcgccgc catcaccata cccgcagcga ccgcttcacc atgtaaccaa ttgccatacc
    46681 ccatttcagc ttcaatggca tgaccaaagg tgtgtcccag attcagtaaa gcacgtaacc
    46741 cggtttcgcg ctcgtcggca gcgacaactt ctgccttcag ttcacaacaa cggcgaatac
    46801 agtacgccat tgccggaccg tccagacgca acaacgcatc cagattctct tccagccagt
    46861 taaaaaacgc accgtcaaga ataatgccgt atttgatgac ttctgccagc cccgacgcta
    46921 actcacgcgg gggaagcgtt ttcagacagt cgagatccac caccactgaa gcaggctggt
    46981 agaacgcgcc aatcatgttt ttaccgaggg gatggttgac cgcagttttg ccgccaacgg
    47041 aggaatcgac ctgcgacagt aacgtcgtcg ggacttgaat gaaacgaaca ccgcgctgat
    47101 aactcgccgc cgcgaagccg gtcagatcgc ccactacgcc gccgccaagc gccaccagcg
    47161 tagtatcgcg accatgcggc ttttgtaaca acgccgtaaa gacggtatcg agtacagcta
    47221 ggcttttata ctgctcgccg tcagggagga taacgctatc gacgttaaca cccgcctgtt
    47281 caagtacgcc gcggacctta tcgagataca gaggagccag ggtttcgctg gtgaccaaca
    47341 tgacctgctc gcccgatttc agcggtaaga atgaagctgg ttcattaaac aaaccagatg
    47401 cgatggtaat tgggtaacta cgttccccga gagtaacgac aatcctctcc ataacgcgac
    47461 atccacctta attactgtac ccgcagacga gtgtatataa agccagaatt agttgctttc
    47521 cagcatgtga ataatctggt ttgcaaccac tttagcgctt tgatcatcag tacgaatggt
    47581 cacgtcggca atctcttcat acagcggatt gcgttcattg gccaacgctt ccagaacttc
    47641 acgcggcggt gtttcaacgt gcagcaacgg gcgtttttta tcacgctgcg tgcgtgcaag
    47701 ttgcttttcg atggtcgttt caagataaac gacaacgcca cgagcggaaa gacggttacg
    47761 cgtttcacgg gatttcacag agccgccgcc agtagccagc acaataccct gtttctcggt
    47821 caactcattg atgacctttt cttcgcgatc gcggaagcct tcttcgcctt ctaaatcgaa
    47881 aacccagccc acatcagctc cggttcgttt ctcaatctct tgatcggaat cgtaaaattc
    47941 catattgagt ttttgagcta actggcgccc aatagtgctt tttccggcac ccataggccc
    48001 aaccagaaag atattgcgtt tctctgccat tttttcggta ctactaagac tattcgttaa
    48061 tgataaaccc gcttcgctca gagagcgccg caggacatga actgaaacct cataagatat
    48121 tgcgagagtc agactgaaaa ttatctcaat actccagcgg gtttggcaac tgaataaatc
    48181 acccatccct ctgcatatct ggtcgctgca agcgcactgc cttgctacca ccgctctggc
    48241 gataaatcac cgggtaagat tagcgtaaaa aagacagcaa aatgccgcct gaatgataaa
    48301 tcatcatcat ggaacgcagg cagcgaagag caccaactct caaatcggta ctccttgtat
    48361 gctaaatacc tgcgcgcgtc aaatagatga aacacgttca gcataaaaac attaccgctt
    48421 ttacggctgt ttactcactg gaaaccagtc gtggcgtgat aaacaccact aactcgcgtc
    48481 gttcatcttc ttttccgtca tgacgaaata attgcccgaa ccagggaatg tcgccaagca
    48541 acggtacgct atcctgaccc gatttatttt tacgggtaaa aatgccgccc agcgccaacg
    48601 tttctccgct ttttacctcg acctgcgttt cgatctcctg cttatcaatc gccagcactt
    48661 cgccatcggc ctgctgtagc acctgccccg gaacgttctg gctgatgtgt aatttcagcc
    48721 ggatgcgacc tttttgtaac accgtgggcg tgacctccat ccccaggacg gcctctttaa
    48781 attccaccga cgtcgcgcca ctttccccgc tggaaacctg atatggaatt tcgctcccct
    48841 gtttaatgct ggcaggctga agatgtgagg ccagcagacg cggactggcg ataatatcca
    48901 gctgctgttt ttgttcgagc gcggaaagct caagatccag caagcgtccg ttgatgcgcc
    48961 caatgttaaa accgacatgc gttgtcgccg tcgctacgga gaggtcgcta ccaagcgtgg
    49021 tgacttgccc aacgccacca gcgtgttgcg catcggccag cgtccatttc acgcctaact
    49081 cacgcaaact tttttcatta atggtgacaa tatgcgccga cagctcaacc tgcccgaccg
    49141 gcagatccat ttgcgctacc cactgttcaa gcgcgcttaa cgccgttttg ttatctcgta
    49201 gcaaaaggcg attggtgcgt ttatcgacgg tcatactccc tttggcactc agtagcttct
    49261 cccccgcttt cgccagttct cccgcgtcgg cgtattgcag ggttatactg cgattttcca
    49321 gcggcagatt tgcctgcgcc cgcgcctgct ccgcctcctg gcgggcgata ttgtcattct
    49381 gccaggcaat ggaatgcact gagagaatgt tgccttcctg ccgcgttatc agtccggcgc
    49441 ttttcactac agtttgtagt gcctgcttcc agggaacatc tgttagatgt aacgacaccg
    49501 taccgctgac gtctggcgac acgaccaggt tcaacttctc ctgttcagcc agcgcctgca
    49561 acacctgagc taccggaacg tcatccacca tcagcgtcac tttttgcggc tttgccgcct
    49621 gtacgccgga tatcagcatc aacagtagtg cggctatcca ttgcttcatt tgtatctcct
    49681 tgccgttgcc acaaccattg tggcggttcg cagtttgtcc cggtacccag cgttagtacg
    49741 tctggcgtca gctgtaaaat tgtccagccg ttttccagca catcgttttg ctgcacccgt
    49801 cgccatttct tttgcccgtc ttttattaca ccgatgatgc gctcgcctcg ccctaccatc
    49861 ccctgatagc gccactggct aagttcgcta atccggcata gatcttccgg cgatttaaaa
    49921 gggtcacgca taccggttaa aaggcacaat gcaatacctg ccaacaacca gcgtttaacc
    49981 ctcatgcggc atctccagtt gtagcgtgaa caaaagatca tcaccttcca cgcttaacga
    50041 aaaacggctc acgctgacgt tgcgctctgc cagccgtgta aatgccgacg gcactgcttc
    50101 ccacagcgtt ttcaacgcca actcgcctcc ctgcgcggat ggatgccagg aaaccagttg
    50161 cgcgccggat aactgaaaat ccagtggcga aaagggcagc gttttttcct cgctgaaggg
    50221 agtggtgtct accaggcgat acagtgcggc ccactgctgg tgatgacttg cccgtagtcg
    50281 aattaatgcg tcgcgctctt catggtgtgt cgatgataga aaaatgagcg taactaacat
    50341 cagcaaccag aatgcccagc aaaactggcg gaggcggggt gatgtggcga accaccagtc
    50401 aaagaacatg ttcatcgcta accttccttg ttaactgata ctcaaattgc cagcgtccct
    50461 gcgcatcctg ctgcgtggct ccccgctgat tgagatgaaa agacgcatcc tggcggagtg
    50521 acgtttctag tgcgtttaac gcggtaatgc ttgttgtcag ccccttgatc tccagcgttc
    50581 cctgctgcca gcttatcgtt gtcagccagg cgtgctcggg taaaagcgcc gccagtgctt
    50641 ccagcgcaga ttgccagtcg cgggtaaatt gtcgctggcg ctgccgttgc gaacgctgtt
    50701 cgcgtaactg ctgttgctcc agcaaacgtg gcttcgttat ctgtaagctg cgggcgagtt
    50761 gttgttccgc ctgaagcaaa actgcgtcta tgcgcgcttc ggcgctgcct gtcagacgca
    50821 gtattagcgt tatcccgacg gccagcagca gaggcgcaac gaacatcagc aaccagaaac
    50881 gcagaaaagc ggtccggcgt tgctgtcgcc agggcaaaaa attaattggc gggttcatca
    50941 gtacgccttc ccaagcgcca gccccagcgc gatggcaaag tctccaccag gcggcggtag
    51001 cggcggctga cgaacagaaa cggcctccca ggggtcaaat ccgccttcgc cacatatcgc
    51061 gacgctgtcg ggatcaacgg ataacgctgc cgccagctcc ttcgcgctag tcatccccac
    51121 cgccagtttg cgcccccagc tatagcgcgt cgcccacagc cactgttcgt tatcacgcca
    51181 ggccagacat tgctgatgag aaggtaaaaa aggcaggaat cgctgtaatg cactggcatc
    51241 cggggtgatc gcactaacat gaacacgcaa cctttctgcc agcgtaagca gcgttgccag
    51301 ctctttgctt tgcgcggcag tcacgttata agcggggctg agtgagtctt cgctgtaatc
    51361 gaagcgcagg gagtccggat ccatatccag ctcgcgggcc atcgtccctg acagccaggc
    51421 cgtttgctcc cgctcaccaa gggacatcga cgggcgcgga aatgaccgct gtaatgtgcg
    51481 actggcggga aacgccaaca taatgtgatg acgctgcggc agttcgcgac tccacggtaa
    51541 caacgtttta gccagctgct gcgcatcaac aatccgccca tctttgataa tgtcgtgcgc
    51601 cagcggcaac cgccaccagc gttgcaaaaa gcattctttt gcgccccgca cgatcgcaac
    51661 cgctgccact tcttgctgtt gtaaatgcaa accaatttgc cagatcttaa atgtcattgt
    51721 gatgatctcc ttatcacccg tcactctgac gggtatatca atgcgtctgg cttgccttta
    51781 tactaccgcg cgtttgttta taaactgccc aaatgaaact aaatgggaaa tttccagtga
    51841 agttcgtaaa gtattttttg atccttgcag tctgttgcat tctgctggga gcaggctcga
    51901 tttatggcct ataccgctac atcgagccac aactgccgga tgtggtgaca ttaaaagatg
    51961 ttcgcctgca aattccgatg cagatttaca gcgccgatgg cgagctgatt gctcaatacg
    52021 gtgagaaacg tcgtattccg gttacgttgg atcaaatccc accggagatg gtgaaagcct
    52081 ttatcgcgac ggaagacagc cgcttctacg agcatcacgg cgttgacccg gtggggatct
    52141 tccgtgcggc aagtgtggcg ctgttctccg gccacgcgtc acaaggggcg agtaccatta
    52201 cccagcagct ggcgagaaat ttcttcctca gtccagaacg cacgctgatg cgtaagatta
    52261 aggaagtctt cctcgcgatt cgcattgaac agttgctgac gaaagacgaa atcctcgagc
    52321 tttatctgaa caagatttac cttggttacc gcgcctatgg tgtcggtgct gcggcacaag
    52381 tctatttcgg taaaacggtc gaccaactga cgctgaacga aatggcggtg atagccgggt
    52441 tgccgaaagc gccttccacc tttaatccgc tctactcaat ggatcgtgcc gtcgcgcggc
    52501 gtaacgtcgt actgtcgcgg atgctggatg aagggtatat cacccagcaa cagttcgatc
    52561 agacacgcac tgaggcgatt aacgctaatt atcacgcgcc ggagattgct ttctctgcgc
    52621 cgtacctgag cgaaatggtg cgccaggaga tgtataaccg ttatggcgaa agtgcctatg
    52681 aagacggtta tcgcatttac accaccatca cccgcaaagt gcagcaggcc gcgcagcagg
    52741 cggtacgtaa taacgtgctg gactacgaca tgcgccacgg ctatcgcggc ccggcaaatg
    52801 tgctgtggaa agtgggcgag tcggcgtggg ataacaacaa gattaccgat acgctgaagg
    52861 cgctgccgac ctatggtccg ctgctgcctg ccgcagtcac cagcgccaat cctcagggag
    52921 cgacggcgat gctggcggac gggtcgaccg tcgcattgag tatggaaggc gttcgctggg
    52981 cgcgtcctta ccgttcggat actcagcagg gaccgacgcc gcgtaaagtg accgatgttc
    53041 tgcaaacggg tcagcaaatc tgggttcgtc aggttggcga tgcatggtgg ctggcacaag
    53101 tgccggaagt gaactcggcg ctggtgtcga tcaatccgca aaatggtgcc gttatggcgc
    53161 tggtcggtgg ctttgatttc aatcagagca agtttaaccg cgccacccag gcactgcgtc
    53221 aggtgggttc caacatcaaa ccgttcctct acaccgcggc gatggataaa ggtctgacgc
    53281 tggcaagtat gttgaacgat gtgccaattt ctcgctggga tgcaggtgcc ggttctgact
    53341 ggcagccgaa gaactcacca ccgcagtatg ctggtccaat tcgcttacgt caggggctgg
    53401 gtcagtcgaa aaacgtggtg atggtacgtg caatgcgggc gatgggcgtc gactacgctg
    53461 cagaatatct gcaacgcttc ggcttcccgg cgcaaaacat tgtccacacc gaatcgctgg
    53521 cgctgggttc agcgtccttc accccaatgc aggtggcgcg cggctacgcg gtcatggcga
    53581 acggcggctt cctagtggac ccgtggttta tcagcaaaat tgaaaacgat cagggcggcg
    53641 tgattttcga agcgaaaccg aaagtagcct gcccggaatg cgatattcca gtgatttacg
    53701 gtgatacgca gaaatcgaac gtgctggaaa ataacgatgt tgaagatgtc gctatctccc
    53761 gcgagcagca gaatgtttct gtaccaatgc cgcagctgga gcaggcaaat caggcgttag
    53821 tggcgaagac tggcgcgcag gagtacgcac cgcacgtcat caacactccg ctggcattcc
    53881 tgattaagag tgctttgaac accaatatct ttggtgaacc aggctggcag ggtaccggct
    53941 ggcgtgcagg tcgtgatttg cagcgtcgcg atatcggcgg gaaaaccggt accactaaca
    54001 gttcgaaaga tgcgtggttc tcgggttacg gtccgggcgt tgtgacctcg gtctggattg
    54061 gctttgatga tcaccgtcgt aatctcggtc atacaacggc ttccggagcg attaaagatc
    54121 agatctcagg ttacgaaggc ggtgccaaga gtgcccagcc tgcatgggac gcttatatga
    54181 aagccgttct tgaaggtgtg ccggagcagc cgctgacgcc gcctccgggt attgtgacgg
    54241 tgaatatcga tcgcagcacc gggcagttag ctaatggtgg caacagccgc gaagagtatt
    54301 tcatcgaagg tacgcagccg acacaacagg cggtgcacga ggtgggaacg accattatcg
    54361 ataatggtga ggcacaggaa ttgttctgat taaaaaggcg cttcggcgcc ttttcagttg
    54421 gctgacaaag tgcatttgtt tatgccggat gcgggtgaac gcgttatccg gctaacaaaa
    54481 tcgtgaaaaa tcaatatatt gcaggaaccc cataagcctg ataagcgttg tgcatcaggc
    54541 aaacttcacg catttacact cgcccctgcc ctttcaacca ttcgcgcacg aggaacagcg
    54601 cgctgacatt acgcgcttcg ttgaagtcag ggtcttccag caaatccatc atatgcgcca
    54661 gcggccagcg cacctgtggt agcggctctg gctcatcgcc ttccagcgat tccgggtaga
    54721 gatcttgcgc gaccacgata ttcattttac tggaaaagta agacggtgcc atgctgagct
    54781 tcttcaaaaa agtcagatcg ttcgcaccaa atccaacctc ttcttttagc tcgcggttag
    54841 cggcttcgta gacgctttca cccggatcaa ttaatccttt cgaaaaacct aattcgtagg
    54901 attcagttcc cactgcgtat tcgcggatca ggatcaggtg atcgtccaca atcggcacaa
    54961 tcatcactgc ttcccggttg gttggacgca ttcgttcata aacacgccgc acgccattgc
    55021 tgaactccag atccacactc tcgacggtaa acagtcggga acgggctaca gtttcaacat
    55081 tcagaatggt gggtttttgt aatgatttgt tcatcgtggg atctatgctg tgaaatcagc
    55141 ggttattgtg cgatatcgga cacgctttcg gcaatgtgaa ttgcatgtta tttacattta
    55201 tgtaacttaa taaataatcg tcctcaaatc aaattaaaag tcaataggtt gaaataactc
    55261 caggaatttg ctgatattcc gccttcagag ggtttgctat gatcagcggt tactgtgatg
    55321 tgcttaatga tgctcaagtt aaactccacg cttgccgata gccaaccgca gaatcatgta
    55381 ttgtgtccgg tgcgactgac cacgcctgac agactaagta agatggggaa agcatgagca
    55441 ccattgtgat ttttttagct gctttgctgg cctgctcact acttgcggga tggctgataa
    55501 aagtgcgatc cagacggcgt cagctgccct ggaccaacgc cttcgcggat gcgcaaacgc
    55561 gtaaactcac acctgaagaa cgtagcgccg ttgaaaatta tcttgagagc ctgacgcagg
    55621 tattacaggt gcctggccca acgggagcta gcgcggcacc gatctctctg gcgctgaatg
    55681 ccgaaagcaa caacgtcatg atgctgacac acgctatcac acgttacggc atctctaccg
    55741 acgatccgaa taaatggcgt tactacctcg attcggtaga agtccacctg ccccccttct
    55801 gggaacagta catcaacgat gagaataccg ttgaactgat tcataccgat tcgctgccgt
    55861 tggttatttc actcaacggt catacgctgc aggagtacat gcaggaaacg cgcggctatg
    55921 ccttgcaacc tgttccgtcc acgcaggcgt cgattcgcgg ggaagagagt gagcaaatcg
    55981 agctactcaa tattcgcaaa gaaacgcatg aagaatatgc gctgagtcgt ccgcgcgggc
    56041 tgcgtgaagc gttgctgatc gtcgcctcct tcctgatgtt ctttttctgc ctgattaccc
    56101 cggatgtatt tgttccgtgg ctggcaggcg gcgcattact gctgctgggc gcaggtctgt
    56161 gggggctatt cgcgcccccg gcaaaatcct ccctgcgaga aattcattgt ctgcgcggta
    56221 caccccgtcg ttggggattg tttggcgaaa acgatcagga acagatcaac aatatttcac
    56281 tcggtattat cgacctggtc tatcccgcac actggcagcc atacattgct caggatctcg
    56341 gtcaacaaac cgatattgat atctatctag accgccacgt agtgcgtcag gggcgatatc
    56401 tttcgctgca tgatgaagta aaaaactttc cgttacagca ctggctgcgc agtacgatta
    56461 tcgctgcggg ctcgctgctg gtgctgttta tgctgttatt ctggatcccg ctggatatgc
    56521 cgctgaaatt cactctctca tggatgaaag gcgcgcagac cattgaagcc accagcgtaa
    56581 aacaactggc tgacgctggc gtgcgggtag gcgatacatt gcgtattagc ggtacgggaa
    56641 tgtgtaatat tcgcacttcc ggaacctgga gcgcgaaaac caattcacct tttttaccat
    56701 ttgactgctc gcagatcatc tggaatgacg cccgctcatt accattacca gaatctgaac
    56761 tggtcaacaa agcgacggca ttgactgaag cagttaatcg ccagctgcac cctaaaccgg
    56821 aagatgaatc tcgcgtcagt gcctcattac gttcagcgat tcaaaaatcc ggcatggtat
    56881 tgcttgatga ttttggcgac attgtactga agacagcgga tttatgttct gccaaagatg
    56941 actgtgtgcg actgaaaaat gcgctggtca atctcggcaa cagtaaagac tgggacgcgc
    57001 tggtaaaacg cgccaacgct gggaagctcg atggcgtgaa tgtgttatta cgcccggtga
    57061 gtgcggaatc gctggataac ctggtggcaa cctccaccgc gccgtttatc acgcatgaaa
    57121 cggcgcgagc ggcacaatca ctaaacagtc cggcccccgg cggattcctg attgtcagcg
    57181 acgaaggcag cgattttgtt gatcagccct ggccttcggc atcactttac gactacccgc
    57241 cgcaagaaca gtggaacgct ttccagaaac tggcacaaat gctgatgcat acgccgttta
    57301 acgccgaagg tatcgtcaca aaaatcttca ctgacgccaa tggtacgcag catattggcc
    57361 ttcatccgat cccggatcgt tccggcctgt ggcgctatct cagcaccaca ttgctgctac
    57421 tgacgatgct gggtagcgcc atttacaatg gcgtacaggc ctggcgtcgt taccagcgtc
    57481 atcgcactcg catgatggag attcaggcct attatgaaag ctgcctgaac ccgcaactga
    57541 tcaccccttc agaaagcctt atcgaataac acgtttgcgc ggcaggttat gctaccctgt
    57601 cgcgcaaatt gcttcactct ggagatttcc ctcatgcata tcaacattgc ctggcaggac
    57661 gtagataccg ttctgctgga tatggacggc acgttgctcg acctcgcctt cgataactat
    57721 ttctggcaaa agctggtgcc tgaaacatgg ggcgcgaaaa acggggttac gccacaggaa
    57781 gcgatggaat atatgcgcca gcaatatcac gacgtacagc atacgctaaa ctggtactgt
    57841 cttgattact ggagtgagca actgggtctg gatatctgtg cgatgaccac cgagatggga
    57901 ccgcgcgccg tactgcgtga agataccatt ccgtttcttg aggcactgaa agccagcggt
    57961 aagcagcgaa ttttgctcac caatgctcat ccgcacaacc tggcggtaaa acttgagcat
    58021 acgggtctgg acgcacacct tgatttatta ctttccaccc acacatttgg ttatccgaaa
    58081 gaggatcagc ggttatggca tgcggtggcc gaagctacgg gtctgaaagc tgaaagaacg
    58141 ctgtttattg atgacagcga agcgattctc gatgctgccg cgcaatttgg tattcgttac
    58201 tgcctcggcg tgactaatcc tgattccggg attgccgaga aacagtatca acgccatccg
    58261 tcactgaatg actaccgccg cctgatcccc tcgctaatgt gaaggagacg ccatgaaaga
    58321 gaaacctgct gttgaggttc gactggataa atggctatgg gctgcccgtt tttataaaac
    58381 ccgcgcgctg gcccgtgaaa tgattaaagg cggtaaggtg cattacaacg ggcagcgcag
    58441 caagccgagc aaaatcgtcg agctgaatgc cacgctcact ctgcgccagg gaaatgacga
    58501 acgcacggtg attgtaaagg cgattactga acagcgtcgc cccgccagcg aggcagcctt
    58561 gctgtatgaa gagactgcgg aaagtgtaga gaaacgcgaa aaaatggcga tggcacgtaa
    58621 acttaatgcc ttaaccatgc cgcacccgga ccgacgcccg gacaaaaaag agcgccgcga
    58681 cctgttacga tttaaacacg gcgacagtga ataactgtca cctgcaagag agatgattat
    58741 gccgcaacat gaccaattac atcgctatct gtttgaaaat tttgccgttc gcggcgaact
    58801 ggtaaccgtt tcggaaaccc tgcaacagat ccttgagaac cacgattatc cgcagcccgt
    58861 taaaaacgtg ctggcagaac tgctggttgc gaccagcctg ttaaccgcta cgctgaagtt
    58921 tgatggtgat atcaccgtac agctgcaggg cgacggtccg atgaatctgg cggttattaa
    58981 cggtaacaat aaccagcaaa tgcgcggtgt ggcgcgcgtg cagggcgaaa ttccagaaaa
    59041 tgccgacctg aaaacgctgg tcggcaatgg ttacgtggtg atcaccatta ccccgagcga
    59101 aggcgaacgc tatcagggcg tagttggtct ggaaggtgat accctggcgg cctgcctaga
    59161 agattacttt atgcgttctg aacagctgcc gacgcgcctg tttattcgca ccggcgacgt
    59221 agacggcaaa ccggctgcag gcggtatgtt gttgcaggta atgcctgcgc aaaataccca
    59281 gcaggacgac tttgaccacc tggcgacgct aaccgaaacc atcaaaaccg aagaactgct
    59341 gaccttaccg gcaaacgaag tgttgtggcg tttgtatcac gaagaagagg tgacggttta
    59401 cgatccgcag gatgtggagt tcaaatgcac ctgctcgcgt gaacgttgcg ccgatgcgct
    59461 gaaaacgctg cctgatgaag aagttgatag catcctggcg gaagatggcg aaattgacat
    59521 gcattgtgat tactgcggta accactatct gttcaatgcg atggatattg ctgaaatccg
    59581 caacaacgcg tctccggcag atccgcaagt tcattaatga gtatgtccgg cagagagggc
    59641 tctgccggat tttacgactt aatggcctga tgtgctgcgc ttaccagcgc ctatgaaata
    59701 aatcgcaaat tattttggtc agatacagcg tcgtatccga taaaagcgtt gtcacttact
    59761 taagtttatt tccagcgttt caccatctac cgtcagatcg cgccacgctt tcggcctcgt
    59821 ccccagtttt ccatccttcg cagctgtgag taatttttct ttcgttattt cgttcggaag
    59881 taaactcata tcaagcgcgt cccattcttt tctgtctgag tcaaagccat agacaatgac
    59941 tctgtcatca ttaaaagcaa acaggatccg ctccgcctga ccatcactat tcagatcttg
    60001 ctcgaccagt acgcaggcgt ctttttcaat acaggtcatc acgttatagc gatcctggat
    60061 taaggccgac cagaatgtcg catccggttt aacagaacca ggggcaatta acacattttc
    60121 ggctaatact ttttccgata cctgttcctg aagatgttgc tctccatcta acgccatcag
    60181 cagatcccgc gcgcgtttcg ggtctttcat aaacccggca tcgcttttca gcgactcaag
    60241 cgccgcacgt ccatagcgac cgctctgttc gagcatgtag atagttacct ggtctggcgt
    60301 gtttttgccg ctctgataac gcgccatatg actgttcacg ctaatacgca tactgtccag
    60361 caccggcgaa ttaagaagca ccagtatcac caacaccaat aacgaaaccg caaggttcac
    60421 tttgccctga agaacaaggg gattttgccc attacgccag acgatactga caaaataccc
    60481 cagcgaccac accagtaaca ccagctccgc cagcgcgccc tgcaagcggt cgacagtcca
    60541 gccgtactga gcgacccgca gccataaccc ccaggtggcg acgaacacat acagcggagc
    60601 aaccagcaaa gcggttttaa tcaggcaacg caacggcccc gtccagggaa gtgacgcttt
    60661 ttgcggatcg cggacaatag ccattaagat caattgcaaa aaggccagcg ttaacagcaa
    60721 cccggcggcg gagatgtggc gagaaatcgc gctcaggccc gtaaacggca gggtgatgat
    60781 aaacatcagg gttagcaatg atactaacgg cagcaacccc gtggcgatta gcgtgaacaa
    60841 cttttgaata gagtcgatta aacgtgactg tgtacgcgcg aggatcaccg ccagtgcggt
    60901 aaccagacca aatgtaagat acataaacca gtcggttgca aaaaagagcg tattgaaaaa
    60961 cgtgatgcca acaagtttaa acaactcacc ccacagtaaa agcaccagcc acgtcaggcc
    61021 attggagaga aaaatcacca gtaatataag tacgttatgc catactgact ggtaaaagta
    61081 gctatagcgg ctactgccat tgcggatacg aaggctttgt tgtatccacg gtagcaacaa
    61141 cattgccatc aacagcagat agcagccaaa atcccaaatc gccttttcag ctctccaggg
    61201 gttcatgcca tcagtttgcc acttcagcca accgctcatc cctgtcgtgg caataaacac
    61261 cagcgccagc catccccaaa ggcgcttctg tttaaaagag atcacggaga agagtaaaac
    61321 ggatgaaaac gcgacggtcg caggcacgcc ataaacaatc caactgtgat tttttccggc
    61381 aagccaggcg ataagcaggt agcaaaccag cccctgaagt aatccggtag caatcatccc
    61441 ccagcgtgtt gccggtgaaa gttcaacgtt atccatgttg gttatccaga atcaaaaggt
    61501 gggttaatta tcgcatccgg gcagtagtat tttgcttttt tcagaaaata atcaaaaaaa
    61561 gttagcgtgg tgaatcgata ctttaccggt tgaatttgca tcaatttcat tcaggaatgc
    61621 gattccactc acaatattcc cgccatataa accaagattt aaccttttga gaacattttc
    61681 cacacctaaa atgctatttc tgcgataata gcaaccgttt cgtgacagga atcacggagt
    61741 tttttgtcaa atatgaattt ctccagatac gtaaatctat gagccttgtc gcggttaaca
    61801 cccccaaaaa gactttacta ttcaggcaat acatattggc taaggagcag tgaaatgcgc
    61861 gttaacaatg gtttgacccc gcaagaactc gaggcttatg gtatcagtga cgtacatgat
    61921 atcgtttaca acccaagcta cgacctgctg tatcaggaag agctcgatcc gagcctgaca
    61981 ggttatgagc gcggggtgtt aactaatctg ggtgccgttg ccgtcgatac cgggatcttc
    62041 accggtcgtt caccaaaaga taagtatatc gtccgtgacg ataccactcg cgatactttc
    62101 tggtgggcag acaaaggcaa aggtaagaac gacaacaaac ctctctctcc ggaaacctgg
    62161 cagcatctga aaggcctggt gaccaggcag ctttccggca aacgtctgtt cgttgtcgac
    62221 gctttctgtg gtgcgaaccc ggatactcgt ctttccgtcc gtttcatcac cgaagtggcc
    62281 tggcaggcgc attttgtcaa aaacatgttt attcgcccga gcgatgaaga gctggcaggt
    62341 ttcaaaccag actttatcgt tatgaacggc gcgaagtgca ctaacccgca gtggaaagaa
    62401 cagggtctga actccgaaaa cttcgtggcg tttaacctga ccgagcgcat gcagctgatt
    62461 ggcggcacct ggtacggcgg cgaaatgaag aaagggatgt tctcgatgat gaactacctg
    62521 ctgccgctga aaggtatcgc ttctatgcac tgctccgcca acgttggcga gaaaggcgat
    62581 gttgcggtgt tcttcggcct ttccggcacc ggtaaaacca ccctttccac cgacccgaaa
    62641 cgccgcctga ttggcgatga cgaacacggc tgggacgatg acggcgtgtt taatttcgaa
    62701 ggcggctgct acgcaaaaac tatcaagctg tcgaaagaag cggaacctga aatctacaac
    62761 gctatccgtc gtgatgcgct gctggaaaac gtcaccgtgc gtgaagatgg cactatcgac
    62821 tttgatgatg gttcaaaaac cgagaacacc cgcgtttctt atccaatcta tcacatcgat
    62881 aacatcgtta agccggtttc caaagcgggc cacgcgacta aggttatctt cctgactgct
    62941 gatgctttcg gcgtgttgcc gccggtttct cgcctgactg ccgatcaaac ccagtatcac
    63001 ttcctctctg gcttcaccgc caaactggcc ggtactgagc gtggcatcac cgaaccgacg
    63061 ccaaccttct ccgcttgctt cggcgcggcg ttcctgtcgc tgcacccgac tcagtacgca
    63121 gaagtgctgg tgaaacgtat gcaggcggcg ggcgcgcagg cttatctggt taacactggc
    63181 tggaacggca ctggcaaacg tatctcgatt aaagataccc gcgccattat cgacgccatc
    63241 ctcaacggtt cactggataa tgcagaaacc ttcactctgc cgatgtttaa cctggcgatc
    63301 ccaaccgaac tgccgggcgt agacacgaag attctcgatc cgcgtaacac ctacgcttct
    63361 ccggaacagt ggcaggaaaa agccgaaacc ctggcgaaac tgtttatcga caacttcgat
    63421 aaatacaccg acacccctgc gggtgccgcg ctggtagcgg ctggtccgaa actgtaatga
    63481 tttgaagctg gagaatatct atccagtatc ttatagaaag caaaacggga ggcaccttcg
    63541 cctcccgttt atttaccctt cttttgtcgt gccctgcgcc cgcgttaccg gcactggcag
    63601 ccaggcgcga atggaaagcc cgccccgctc gctggtgcca agctccagca tcccgttatg
    63661 gttatccacg atacgctgca caattgccag ccctaatccc gtgccgctaa tggtgcgcgc
    63721 actgtcgccg cggacaaacg gctggaacag gtgcttacgt tgttccggcg caattcccgg
    63781 accgtcatct tccacctgga accaggcgcg attcggctcc gttccgctgc tgactttgat
    63841 ccagccattg ccataacggg cggcgttgac caccatattc gccaccgcgc gtttgatcga
    63901 cagcgggtgc attttcactt caatgctgcc ggggtaaagc gcggtttcaa tttcccgctc
    63961 atagccactt tcggcagcaa tcacctcacc gagcactgca ttaagatccg ccatttccat
    64021 cggcatctcc tgcccggtgc gcaggtagtc gataaactgc tcaatgatgg cgttgcactc
    64081 ttcgatatct ttattgatcg attctgccag atagccatcc tgctcgctca tcatctcagt
    64141 cgccaggcga atacgcgtca gcggcgtgcg caagtcgtga cttacccccg ccatcagcag
    64201 cgtgcggtca tccgccagtt gcttaacacc agccgccata tggttaaagg cacgggtaac
    64261 ggaacgcacc tccgaagcgc catattcacg cagcggcggc ggaataatcc ctttaccaac
    64321 ctgcaaggct gcgtgttcga gatcgaccaa cggtcggttc tggatacgaa taaacagcca
    64381 cgccccgcct atcgccaata gcataatcgc cagcgtatag cggaacagcg gagagaaatc
    64441 gccctgatga atttcggtca gcggcacgcg tacccagata ttgggcgaca gccaggtttt
    64501 cagccagacg acaggcgaac ttttgttgac ctcaacgcgc acttccgtcg ggccgcccag
    64561 ttgctgtgcc atctgatggc ttaagaattc atagtgttgc gcccaacgca gacctgcctc
    64621 ttcggcagcc tcgttggagt agagagagat ccccagctca cggtagatct cccgacggaa
    64681 agcgggaggc acaaccaact gcgtgccgtc ctccagttgc agtttatcgg tcatcaacat
    64741 acgcacttcg tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa tcgcgaagtt
    64801 cagcaccacc agataagtcg tcaccaggct ggcgaacagc aaggtgacga tgagcaataa
    64861 cgtacgggca aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt agagccgtcc
    64921 ggtacaaaga cgtagcccag gccccaaacg gtctgaatgt aacgcggatg cgctggatct
    64981 tcttctacca tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc cattgcggaa
    65041 tattcacgac cacgggcaag gttcatcagc ttatcgcggg agagcggctc acgcggatgg
    65101 ctgaccagtg ccttcagtac cgcaaactca ccgctggtga gcggcatcgg ctcatcttcg
    65161 cggaacattt cgcgcgtacc gaggttaagt ttgaacttac cgaaagcaat taccgcctct
    65221 tcctgtgacg gtgcgcctgg cagttcgttc gcctgacgac gcagcaccgc acgaatacgg
    65281 gccagcagtt cacgcgggtt aaacggtttt ggaatgtagt cgtcagcgcc aatctccagg
    65341 cctacgatac ggtccacttc ttcccctttc gccgtcacca taatgatcgg catcgggttg
    65401 ctctgactac gaagacgtcg gcaaatcgac aagccatctt caccaggtaa cattaaatcc
    65461 agtaccataa gatggaaaga ttcacgagtc agcaggcgat ccatctgttc tgcattagcg
    65521 acgcttcgaa cctggaagcc ttgttcggtg agataacgtt ccagcagcgc acgcaggcgc
    65581 atgtcgtcat cgaccaccag aatcttgtag ttctcttgca ttgtttgtac tcccaaaggt
    65641 tcgcaacaat ttgtaagcgt gtattcttaa aaaagctcac gttcgtcacc agctaaatct
    65701 ggtatgaatt tcagcctaaa ttgttacaaa gcatattaaa cagcagctta agtatacaat
    65761 ttattcggcg aaacattatt gattctgttg atatgatcac gttataccca atgtgcgcat
    65821 tatcaaacag acaaagggaa tcaacgagat gaaaacgccc ctggttaccc gggaagggta
    65881 tgaaaaactc aaacaagagc ttaattatct ctggcgtgaa gaacgcccgg aggtcacaaa
    65941 aaaggtgacc tgggccgcaa gtctgggcga ccgcagcgaa aatgctgact atcagtataa
    66001 taaaaagcgt ctgcgtgaaa tcgaccgtcg cgtgcgctat ctcactaaat gcctggaaaa
    66061 tctcaaaatt gtcgattact cccctcagca ggaaggcaaa gtcttttttg gcgcgtgggt
    66121 ggagattgaa aacgacgatg gcgtgactca ccgtttccgt attgtcggct acgatgaaat
    66181 ttttggccgt aaagattaca tctctatcga ttccccgatg gcccgcgcat tgctgaaaaa
    66241 agaagtcggc gatctggcgg tggtgaatac ccctgccggg gaagcgaact ggtatgttaa
    66301 tgctatcgag tacgtgaaac cgtaaggaag agtcttaacc tcctgccgat ggctggcatt
    66361 tttgccagcc agtccgtata actatcccct gatttttgat ccgaaaagat gaactcaaac
    66421 catgatgaat gattcgttct gccgcattat tgcgggtgaa attcaggcgc gcccggaaca
    66481 ggttgacgct gccgttcgcc tgcttgatga agggaatacc gtgccgttta tcgcacgtta
    66541 tcgtaaggaa atcaccggcg gtctggatga cacgcagctg cgtaatctgg aaacgcgtct
    66601 gagctatctg cgcgagctgg aagagagacg tcaggcgatc ctcaaatcca tttccgagca
    66661 aggcaaactc accgatgatc tggcgaaggc catcaacgcc accctaagca aaaccgaact
    66721 cgaagacctc tacctgccct acaaacctaa acgccgcacc cgcgggcaaa tcgccattga
    66781 agcagggctt gagccgttgg ctgacctgct gtggagcgat ccgtcacaca cgccagaagt
    66841 cgccgctgca caatatgttg atgccgataa aggcgtggca gataccaaag ccgcgctgga
    66901 tggcgcgcgc tatatcctga tggaacggtt tgccgaagat gccgcgctgc tggcgaaagt
    66961 gcgtgattat ctgtggaaga acgcgcattt ggtttctacg gtggtgagcg gtaaagaaga
    67021 ggaaggggcg aaattccgcg actatttcga tcatcacgaa ccgttgtcca cggtgccttc
    67081 tcaccgcgcg ctggcgatgt tccgtgggcg taacgaaggc gtactccagc tttcgctgaa
    67141 tgccgatccg cagttcgatg agccgcccaa agagagctat tgcgagcaaa tcatcatgga
    67201 tcaccttggc ctgcgcctga acaatgcccc ggcggatagc tggcgcaaaa gcgtagtgag
    67261 ctggacctgg cgcatcaagg tgctgatgca tctggaaacc gaactgatgg gtaccgtgcg
    67321 cgaacgcgcg gaagatgaag caatcaacgt ctttgcccgt aacctgcacg atctgctgat
    67381 ggcggcccct gccggactgc gtgcaacgat gggcctcgat ccgggtctgc gtaccggggt
    67441 aaaagtggcg gtggtcgatg ccactggcaa actggtggcg accgatacca tttacccgca
    67501 caccggacaa gccgcaaaag cagcgatgac cgttgctgcc ttgtgtgaaa aacataacgt
    67561 tgaactggtg gcgatcggca acggtacagc ttcccgcgaa actgaacgtt tctatctcga
    67621 cgtgcagaag cagttcccga aagtgaccgc acagaaagtg atcgtcagcg aagctggcgc
    67681 gtcggtttac tcagcttccg agctggcagc gcaggagttc ccggatctcg acgtttcgct
    67741 gcgtggtgcg gtatctatcg cccgccgttt gcaggatccg ctggcggagc tggtgaaaat
    67801 cgatccgaaa tctatcggcg taggtcagta tcagcatgac gtcagccaga cgcaactggc
    67861 ccgcaaactg gatgcagtag tagaagactg cgtaaacggc gttggcgtcg atctcaacac
    67921 cgcttctgtt ccgctattaa cccgcgtggc gggcctgacg cgcatgatgg cgcaaaacat
    67981 cgtggcctgg cgcgatgaga acggccagtt ccagaaccgt cagcaactgc tgaaagtcag
    68041 ccgtctgggg ccgaaagcct tcgagcagtg cgcgggcttc ctgcgcatta accacggtga
    68101 taacccgctg gacgcctcta ccgttcatcc ggaagcctat ccggtggtgg aacgcattct
    68161 ggcagcaaca cagcaggcac tgaaagatct gatgggtaac agcagcgaac tgcgtaacct
    68221 gaaagcgtct gactttactg atgaaaaatt cggtgtgccg acagtaactg acatcatcaa
    68281 agagctggaa aaaccgggtc gcgatccgcg tccggaattt aaaaccgctc agtttgccga
    68341 tggcgtcgag acaatgaacg acctgcaacc gggtatgatc ctcgaaggcg cagtgaccaa
    68401 cgtcaccaac tttggcgcgt ttgtcgatat tggcgtgcat caggacggcc tggttcacat
    68461 ctcttcattg tcgaacaagt ttgtggaaga tccgcatacc gtggtgaaag cgggcgacat
    68521 tgtgaaggtg aaagtgctgg aagtggatct tcagcgtaaa cgtatcgccc tgactatgcg
    68581 tctggacgag cagcctggcg aaaccaactc tcgtcgcggc ggcggtaatg aacgcccgca
    68641 aaacaaccgc ccggcagcca aaccacgcgg tcgtgaagcg cagcctgccg gtaatagcgc
    68701 gatgatggat gcgctggcgg cagcgatggg taaaaaacgt taaacgcccg gactggcccc
    68761 taaacctgcg cgataaggcg ttcacgccgc atctggcaac gaacgctttg cctgacataa
    68821 aagtgccgga gaatatctcc ggcactttta ttccacagcc aaactcacaa tatactccgt
    68881 taatatttat cacttcatta acaactgaaa ccttaattaa acattagcca gtccgggtaa
    68941 ctcactattc gaattatatt ttcgctgcga tataaccttg agccacatca acattgagtc
    69001 agattattat tcaaaccaac attcgtacac attttaagta ttgctgatag aaaccattct
    69061 cattatcatt gtgttgttga ttatttaatc tctcctttgt tggcaaatca tctggtctca
    69121 tgtcgcggtc aaacgcccca tgaggtagtt atccagttaa tgagaaacaa gtaggcacct
    69181 atgcaataca ctccagatac tgcgtggaaa atcactggct tttcccgtga aatcagcccg
    69241 gcatatcgcc aaaaactgct ttctctaggc atgttacctg gctcctcttt tgatgtggtg
    69301 cgcgtcgctc cactcggcga ccccattcat atcgaaaccc gtcgtgtgag cctggtatta
    69361 cgcaaaaaag atctggcctt attagaagtg gaagcggttt cctgttaata cggtgataac
    69421 aacaatgaaa aaattaacca ttggcttaat tggtaatcca aattctggca agacaacctt
    69481 atttaaccag ctcactggcg cacgtcagcg tgtaggtaac tgggctggcg ttaccgtcga
    69541 acgtaaagaa gggcaattct ccaccaccga tcatcaggtc acgctggtgg acctgcccgg
    69601 cacctattct ctgaccacca tctcatcgca gacctcgctc gatgagcaaa tcgcctgtca
    69661 ctacattttg agtggcgacg ccgacctgct gattaacgtg gtggatgcgt ctaaccttga
    69721 gcgtaacctg tacctgacgc tgcaactgct ggaactcggc attccctgca tcgtggcgct
    69781 gaacatgctc gacattgccg agaagcaaaa tattcgtatt gaaattgatg ctctatcggc
    69841 acgtcttggc tgcccggtga tcccgctggt ttcaacccgt ggtcgcggta ttgaagcgct
    69901 caagctggcg attgatcgct ataaagctaa cgagaatgtg gaactggtgc attacgcaca
    69961 gccgctactc aacgaagcag attcactggc aaaagtgatg ccttccgaca tcccgctgaa
    70021 acaacgtcgc tggctgggcc tgcaaatgct ggaaggcgat atctacagcc gcgcctacgc
    70081 cggtgaagcg tcgcagcatc tggatgccac cctcgcccgt ctgcgtaatg aaatggatga
    70141 tccggcgctg cacattgccg atgcgcgtta ccagtgcatt gctgccatct gtgatgtggt
    70201 aagcaacacc ctgacggcag aacccagccg tttcaccact gcggtagata aaatcgtgct
    70261 caaccgtttc ctcggtctgc cgattttcct ctttgtgatg tacctgatgt tcctgctggc
    70321 tatcaacatc ggcggggcgt tacagccgct gtttgacgtc ggctccgtgg cgctatttgt
    70381 gcatggtatt caatggattg gctacacgct ccacttcccg gactggctga ctatcttcct
    70441 cgcccagggc atgggtggcg gtattaacac cgtgctgcca ctggtgccgc agatcggcat
    70501 gatgtacctg ttcctctcct tccttgagga ctccggctat atggcgcgtg cagcgtttgt
    70561 gatggaccgc ctgatgcagg cgctgggctt gccggggaaa tcctttgtgc cgctgatcgt
    70621 cggtttcggt tgtaacgtac cgtcggtaat gggcgcacgt acgcttgatg caccgcgtga
    70681 acgtctgatg accatcatga tggcaccgtt tatgtcctgc ggcgcgcgtc tggctatctt
    70741 cgcagtattc gcggctgcct tcttcgggca gaacggtgcc ctggcggttt tctcgctgta
    70801 tatgctcggt atcgtgatgg cggtgctgac tggcctgatg ctcaagtaca ccatcatgcg
    70861 cggtgaagca acgccgtttg tcatggagct gccggtatac catgtaccac acgttaaaag
    70921 cctgattatc cagacctggc agcgtctgaa gggcttcgtt ctgcgtgctg gtaaagtgat
    70981 tatcatcgtc agcattttcc tgagcgcttt caacagcttc tcgttgagcg ggaaaatcgt
    71041 cgataacatc aacgactcgg cgctggcttc cgtcagccgg gtgataaccc ctgtcttcaa
    71101 gccgattggc gtacatgaag ataactggca ggcaacggtt ggcctgttta cgggcgcaat
    71161 ggcaaaagaa gtggtggtgg gtacgctcaa caccctctac accgcagaaa atattcagga
    71221 cgaagagttc aatccggcgg aattcaacct cggtgaagag ctgttcagtg cggtagatga
    71281 aacctggcag agcctgaaag acaccttcag ccttagcgta ctgatgaacc ccattgaagc
    71341 tagcaaaggc gacggtgaaa tgggtaccgg ggcgatgggc gtgatggatc agaaattcgg
    71401 tagcgcagct gccgcataca gctacctgat tttcgtcctg ctgtatgtac catgtatctc
    71461 ggtgatggga gccatcgccc gtgaatcaag ccgtggctgg atgggcttct ccatcctgtg
    71521 ggggctgaat atcgcttact cactggcaac attgttctat caggtcgcca gctacagtca
    71581 gcacccaact tacagcctgg tgtgcattct ggcggtaatc ctgtttaaca tcgtagttat
    71641 cggtctgctg cgccgcgcgc gtagccgggt ggatatcgaa ctgctggcaa cccgcaagtc
    71701 ggtaagcagt tgctgcgcag ccagcaccac cggtgattgc cattaatggc ttcacttatt
    71761 caggtacgtg atttgctggc gttacggggc cgtatggaag cgacccagat aagccagaca
    71821 ttgaacactc cgcagccaat gattaacgcc atgctgcaac agctggaaag catgggcaaa
    71881 gccgtgcgga ttcaggaaga acctgacggc tgcctctctg gcagttgtaa aagctgcccg
    71941 gaaggcaaag cctgtctgcg cgagtggtgg gcgctgcgtt aaccatactc catcgcctgt
    72001 tttgaaaagc ccggtatgcg tttgcatccg ggcttttttg cgtgcggctt tccataaaaa
    72061 tgcaattctt gcagcgcaac gtaagttcct ctgaaagcgt ctcgcaggga tgaaaactcg
    72121 ctaatgcaca gatgtggagt ggcgcgtaga gtcgcggcat tcaaacaaca ggtgaaggaa
    72181 cgccatgagt aaaaagcaga gttccacccc acatgatgcg ctgttcaaac tctttttacg
    72241 ccaaccggag acggcacgcg attttcttgc gtttcattta ccggcaccca ttcacgcgct
    72301 ctgtgatatg aaaaccctca agctggagtc gagcagcttt attgatgacg atctgcgtga
    72361 aagctattcc gatgtgctgt ggtcggtgaa aacggaacaa ggaccaggat acatctattg
    72421 tctgattgaa catcaaagca cctcaaacaa actgatcgca tttcgcatga tgcgttacgc
    72481 tattgccgca atgcaaaatc accttgatgc tggatacaaa acgttgccga tggtggtgcc
    72541 attgttgttt taccacggta ttgaaagccc ctatccctat tcgctgtgtt ggctggattg
    72601 tttcgccgat cccaaactgg caaggcagct ttatgcctcc gcatttccgc tgattgatgt
    72661 caccgtcatg cctgatgatg aaatcatgca gcaccgacgt atggcgctgc tggagttaat
    72721 tcaaaaacat attcgtcaac gcgatctgat ggggctggta gagcaaatgg cctgcttatt
    72781 aagtagtgga tacgctaatg acagacaaat caaagggctg tttaattaca tactgcaaac
    72841 tggcgacgct gtacgtttta acgattttat cgacggcgtt gccgaacgtt caccgaaaca
    72901 caaggagagt ttaatgacta ttgcggaaag attgcggcag gagggggaac aatccaaagc
    72961 cctgcatata gccaaaataa tgcttgaatc cggagtccct cttgcagaca tcatgcgatt
    73021 taccgggctg tcagaagaag agttggctgc ggcgagtcag tagatactct accctgctta
    73081 agtgctactt tgtgctatct tgcatgtaac acaaagtagc acttagggaa ctgatatgca
    73141 gacagtgaaa ttacggcaac aaggaggagc catgatcgtt actatcccac gagatcttgc
    73201 catcgatctt ggctggtcac cgggcacaga gcttaccgtg gagaaaaaag gcgatagcgt
    73261 taatttgcgc gccacagagc acaaaccgcg tgggcgtctt accgtcgcgc agttgttgag
    73321 ccaaatcgat gaaagcgaaa ttacggagct aaatcagtcc acagaaggat gggctgaagg
    73381 gaaaaaaggt aatgaagcat ggtaaaggca cggacgccac atcgtggtga gatctggtat
    73441 tttaaccctg atccggttgc cgggcatgaa cttcaggggc cacattattg cattgtggta
    73501 acggacaaaa aactcaacaa tgttttaaaa gttgctatgt gctgcccgat ttcaacaggg
    73561 gcaaatgcag cacgttccac aggggtgacg gtgaacgtcc tcccctgtga tacgcaaacc
    73621 ggtaacctgc atggcgttgt actttgtcac cagctaaaag ccgtcgatct tattgcccgt
    73681 ggcgctaaat ttcataccgt tgccgatgaa aaattgatta gtgaagttat cagtaaactg
    73741 gtgaatttaa tcgacccaca ataattagct ctcacttacc accaacaccc tctgcttcaa
    73801 cgccaccagc aggtgacaaa actcgaccgg atgcgaaata aacggcgcat gggccgcttt
    73861 ggcgaagata tatgattcgc tgtgaggcca aagtttatcc agcatcggca ccactttgcg
    73921 cggcaccaga ccgtcgagat agccatacaa tcgcaaaaac ggcatggaca cgttttgcag
    73981 cggctgacgg agatcgaccg ttttcaggat ttccagcccg ccattaagca cgtcaacctc
    74041 cggcatcggt aacgccagaa cggttttctt caacgcccgc gcatcctggc gcgccgtttc
    74101 agtccccatg gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat catcactgag
    74161 ttgctgctga aatcccgcca gcacgtccgg ttttatcccc ggccactcgt cacgagcact
    74221 aaaataaggt gacgacgcca cggtgaccag cgcctgaaca cgctcgggat gggttaacgc
    74281 aatctggctt gccaccagcc cgcccagact ccagcctaac caaatggctt tatcaggtgc
    74341 ctgttgcagc acggcttcgg ccatatcagc aagtgacagc gcaccaaatc cccggctacg
    74401 cccgaagccg ggcaggtcaa caaggtgcag cgtaaaatgc gagctaagtt cctcgtcaat
    74461 gcaacgccac acttcggcat tcagtcccca tccgtgcagc agcacaagat gaacattccc
    74521 ctgacctttg gtctgccacc agatgttatt catccgctat tgttctcttt tgacttacaa
    74581 ggatgaacat atgctaacag taccgggatt atgctggcta tgccgaatgc cactggcgtt
    74641 aggtcattgg gggatttgtt cggtctgttc acgcgccacc cgcacagata aaacgttatg
    74701 cccacaatgt ggattacccg ccacacactc ccatcttccc tgcggtcgct gcctgcaaaa
    74761 accgccgccc tggcaaagac tggtcacggt tgccgactat gcgccgccgt taagtccgct
    74821 tatccaccag cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt cacgtctgtt
    74881 actattggaa gtcttacacg ctcgtcgcac caccgggttg caattgccgg atcgcatcat
    74941 cagcgttccg ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga gcgatttgct
    75001 gtgtcagccg ttatcacgct ggttgcactg ccaatgggat agcgaagccg tcacacgtac
    75061 acgggccact gcgacccagc attttctcag tgcccggctg cgcaagcgca acctgaaaaa
    75121 tgcctttcgt cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg atgatgtcgt
    75181 taccaccgga agtaccgtcg cagagattgc gcagttgctt ttacgcaatg gtgcggcgac
    75241 tgtccaggtc tggtgccttt gtcgaacctt gtagagcctc gatgatgggc gtattataac
    75301 caactaaaat agtcaactat taggccatta ctatgatccg tatttccgat gctgcacaag
    75361 cgcactttgc caaactgctg gcaaatcagg aagaagggac acaaatccgc gtatttgtga
    75421 ttaaccctgg cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg gatgccgtgg
    75481 aagccaccga cactgccctg aaatttgacc tgctgaccgc gtatgttgat gagttaagcg
    75541 caccatacct ggaagatgca gagatcgact ttgttaccga ccagttgggt tctcagttaa
    75601 cgctgaaagc cccgaacgcc aaaatgcgta aagtggcaga cgatgcaccg ctgatggagc
    75661 gcgtggagta tatgctgcag tcgcagatca acccacagct tgctggtcac ggtggtcgcg
    75721 tttcgctgat ggaaatcacc gaagacggtt acgccattct gcaatttggc ggcggctgta
    75781 acggttgttc catggtcgat gtgacgctga aagaagggat cgagaagcag ctgctgaacg
    75841 aattcccgga gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc gaacactcct
    75901 actactaagt tatcccctct tttgtagatt gcccgatgcg acgctaaagc gtcatttcgg
    75961 gcctacgttg aacatgtgcc agacgttggc acagcattgc tttggcaaat ccctcccttc
    76021 gtttccccgc gataatatga ccaacctctc ataatttaaa tttaccccgc tctggtgatt
    76081 ctcaaacgcc agatgttacc cgtatcattc acatgggtac caaacatact cctgacatct
    76141 gactacaata attagtttta gtgggtatca gtcgtggtgc cgcaatatct ctgttcccga
    76201 ttgggataat tagagtttgt cgtcagaaaa ttgacattac ccataacaaa tgaaaggcca
    76261 ggtaaatcat gccattagtc attgttgcta tcggtgtaat cttgttgttg ctcctgatga
    76321 tccgcttcaa aatgaacggc ttcatcgctc tcgtcctcgt ggcgcttgct gttggattaa
    76381 tgcaaggaat gccgctggat aaagttattg gctccatcaa agccggtgtc ggcgggacgc
    76441 tcggtagcct tgccctgatc atgggttttg gcgcaatgct ggcaaactgc ggtggcgcac
    76501 aacgtatcgc caccacgctg attgccaaat ttggtaaaaa gcacatccag tgggcggtgg
    76561 tgttaaccgg ttttaccgtt ggttttgccc tgttctatga agtgggcttc gtgctgatgc
    76621 tgccgctggt gtttaccatc gcggcttccg cgaatattcc attgctgtat gttggtgtac
    76681 caatggcggc tgcactgtct gtgacccacg gcttcctgcc accgcatccg ggcccgactg
    76741 cgattgccac cattttcaat gccgatatgg gtaaaaccct gctgtacggt actattctgg
    76801 caatcccgac cgtgattctc gccggtccgg tttacgctcg cgtgttgaaa ggtatcgata
    76861 agccaatccc ggaaggtctc tacagcgcga aaaccttcag cgaagaagag atgccgagct
    76921 ttggcgtcag cgtctggacc tctctggtgc cggttgtact gatggcgatg cgtgcgattg
    76981 ccgaaatgat cctgccgaaa ggtcacgctt tcctgccggt agcggagttc ctcggtgacc
    77041 cggtaatggc aacgctgatt gccgtgctga ttgcgatgtt cacctttggt ctgaaccgtg
    77101 gtcgttcaat ggatcagatt aacgacacgc tggtttcttc catcaaaatc attgcgatga
    77161 tgctgttgat catcggtggt ggcggtgcgt tcaagcaggt gctggtagac agcggcgtgg
    77221 acaaatacat tgcttccatg atgcacgaaa ccaacatttc tccgctgctg atggcctggt
    77281 cgattgctgc cgtactgcgt atcgcgctgg gttccgcaac cgttgcggca atcactgcgg
    77341 gtggtatcgc ggcaccgctg attgcaacga cgggtgttag cccagagctg atggttattg
    77401 cggtgggttc cggtagtgtg attttctctc atgtgaacga tccgggcttc tggctgttca
    77461 aagagtactt taacctgact atcggcgaga ccatcaaatc ctggtcgatg ctggaaacca
    77521 ttatctcggt gtgcggtctg gtaggctgtc tgctgctgaa tatggtgatt tgataacact
    77581 gactgccgga tgcatcgcat ccgacatcaa ccgcactcta cttcttcttc gctgcagctc
    77641 tgcgccgtct gtccaaatcc ttcagcaact tgttcacgcc atcatcggca aacatcgact
    77701 caagcgttgc ggaaagcttg cgtcgccagt ttttatactg gtaactggtg ccaggaatat
    77761 tcaccggttc ggccatatcc agccagtctt ccggctgtag tcctaacaga gcactgttac
    77821 tgtcggcaat gtagcgctgc aaaccacggt tcagcgtcgt cgtcatcgac atcaacgatg
    77881 ccttatgccc ggcacgtttt ggcagacaac catatttatg cagtgcatcc agcagccctt
    77941 gcttcgccag ttcgcgatcc tgatacagac cgcgcagtac cacttcatcc ggatacagcc
    78001 ccagggtttt gcccagcgtt agatccccgc tctcccagta accacgcagc gttggcaggt
    78061 catgtgtcgc cgcaaccgcc atcgactgct ccggatacgc tttcggtgca cggaacgtct
    78121 tctcgtggtc gttttcgaaa tagagcactt tgtaagagta cacaccgctg ctgcgcagct
    78181 taccgacaat ctctaccggt acggtaccga gatcttcacc aatcaccata cagcgatgac
    78241 gtttactttc gagtgccaga atcgagagca gatcatccac cggatagtga acatacgcgc
    78301 cctgatctgc cgtctcgcca tacggtatcc accacaaacg cagcatcgac atcacatggt
    78361 caattcgtaa tgcgccgcag ttttgcatat tggcacgcaa cagctcgata aacggttcat
    78421 aggcacgcgc ggtgatgata tgcgggtcca ttggcggtaa tccccagttc tgccccaacg
    78481 ggccgaggat atccggcggc gcgccaaccg atgctttcag gcaatatagt tcacggtcac
    78541 accaggtttc cgccccacct tccgctacgc caaccgccag atcacgatac aagccaatcg
    78601 gcatttcata gccctggctt atctcccagc aggcggcaaa ctggctgtaa gccagccact
    78661 gcaaccagag ataaaaatcg acgtcatcac gatgttcttc gcagaactga cgcacttctg
    78721 gtgaatccac gttcttatac atctctggcc atgcaggcca gccccagcgc atttcgtcct
    78781 ctttcacttg ctgggcatgt agcgcatcaa aggctgcctg ccagaacagg ctgtcgccct
    78841 gctctgcaac aaactggcga aacgcggcca tctgctcatc atcacgttgc gcgaaacctt
    78901 tccacgccat tcgtaatgct gtcattttta gggcggtaac cgtggagtaa tcgacccagt
    78961 cggcatcgcg cgcctgttgc agcgtctgtt gcgtggtcgg caactgccac caggcctgag
    79021 cctcttcgct aagatggaaa tcttcaacgg cgttaacgtc gatataaatc acattcagcc
    79081 aacggcgaga agacgggctg tatgggctgg cgctctccgg atttgccgga tagagcgcat
    79141 gaatcgggtt caggccaatg aacgacccgc cacgttttgc cacatccacc agcatcgctt
    79201 tgagatcgcc aaaatcccca ataccccagt ttttttccga tcgcagcgta taaagctgaa
    79261 cgcaggcacc ccacagcttt tgtttattca gcaaagcctg cggttcgtaa caacgtttcg
    79321 gggcgacaat cacccggcaa tgcgcgcgct ggtcgtcctg ggtgagtgtt agcgtgtgat
    79381 aaccttccgg cagcttcgtc ggtagattga acgctttgcc ccccgttaca tggcctttgt
    79441 actgcgttcc ttcttcggtg gtcagctgcc agctatattc gccgctgccc tccaccacca
    79501 tcggcatttt tttgccgctg gtataaacca tgacattcgg gactggcgtt accgccactt
    79561 tcgtggcggt acgttgatgc atcgcgtcaa gcaaacgccg tttggtttcg gcgctaatcg
    79621 actgcggttt accgtgggca ttgatgtaat tggggctaat ccccgccgcc agcgcggcat
    79681 tatccagacg tttgctttcc atcgagcttc cttagcgttt tgcctgccag atacgagcct
    79741 gataatcgcg aatagagcga tccgagctaa acataccgca gcgggcggta ttgaggatcg
    79801 ccgcgcgagt ccaggcctcc tggtcgcggt acagcacatc cacctgcttt tgtgcctcaa
    79861 cataggctgc gaaatccgcc atcaccagat acggatcgcc gccctgtttg ccgatactgt
    79921 gcagcatctg gtcgaaggca tgcttatcgc cgtcgctgta tttaccgctt tccagctctt
    79981 tcaatactgc gtccagcacc ttatctttct tccgccattt caccgggtcg tagcctttgg
    80041 ccagaatagc cttcacttgt tccacggtat gaccaaaaat aaagatattt tcttcaccga
    80101 ctttctcggc gatttcaacg ttcgcaccat ccagcgtacc gacagtaagc gcaccattga
    80161 gcgccagttt catattgccg gtaccggaag cttctttacc tgcagtcgaa atttgttcgg
    80221 agatatccgc cgccgggatc agtttttccg ccgccgaaac gcaataatcc ggcaggaaca
    80281 ccaccttcaa cttatcgcca accagtggat cgttgttgat cacgtcagcc actttgttga
    80341 tcgcaaagat aatattcttc gccaggtagt agcccggtgc cgctttcgcg ccgaagagga
    80401 agacgcgcgg tacgcgatca gcctgcaggt tttcacgaat ttctttgtac aacgccagaa
    80461 tatgcagcag attcaggtgc tggcgtttgt actcatgcag acgtttgatc tgaatatcga
    80521 aaatcgcctg tggattgatc tcaataccgg tacgaacttt cacaaactcc gccagacgga
    80581 ctttattcgc ctgcttgatc tcgcgatatt gctgacggaa tttcgcatca tcagcgaatt
    80641 tttccagatt gatcagctga tcgagatcgt tagcccactc tttttgcagt gatttatcca
    80701 acagagccgc cagtgcaggg ttgcactgtt tgatccagcg acgtggggta ataccgttgg
    80761 tgacgttatg gaatttgttc ggccatagct ggtgatattc cgggaacaga tctttcacca
    80821 ccagatccga gtgcagcgcc gcaacaccgt tcaccgcgaa accgccaacc acacacaggt
    80881 tcgccatatg cacttgttta tcgtgcacca ccgccagttt ggcccacact ttttcatcgc
    80941 ccggccaggt tttctccacc agcgttttaa agcgagtatt aatttcgtta ataatctgca
    81001 tatggcgcgg cagtaagcct ttcaccagtt tcacatccca gcgttccagc gcttctggca
    81061 tcagggtatg gttggtgtaa gcgaaagttt tgctggtgat ggcccaggcg tcatcccagc
    81121 tcatctggtg ctcatcggtc agcacgcgca gcagttctgg aatcgcgata gttggatggg
    81181 tatcgttcag ctgaataact tcgtaatccg ccagttcgtg cagtttacgc cccgccagat
    81241 gatggcgacg caaaatatcc gctaccgaac aggcacactg gaagtattgc tgcatcaggc
    81301 gcagcttttt accggcagta tggttgtcgt ttggatagag aactttggtc agtttttccg
    81361 cattgatgcc ttgctgttcg gcgcgcagga aatcaccgtc gttaaattta gttagatcaa
    81421 acggatgcgc atgcgtcgcc tgccacagac gcagcggctg cgccacgcca ttacgatagc
    81481 cgacaacggg gagatcccac gcttgaccgg taatggtaaa ctccggctcc cagcgtccgt
    81541 ctttcatcac tttaccgcca atccctacct gcacatccag tgcttcgttg tggcggaacc
    81601 acgggtagtt actgcgatgc cagtcatccg gcgcttcaac ctgtttgcca tcgacaaaag
    81661 actggcggaa caaaccatat tgatagttca gaccgtaacc cgtcgcagac tgaccgacag
    81721 ttgccattga gtcgaggaag cacgccgcca gacgtcccag accaccgtta cccagcgccg
    81781 ggtcgatctc ttcttccagc aggtcagtca ggttgatgtc ataagccttc aacgaatcct
    81841 gtacatcctg ataccagccg agattcaaca ggttgttgcc cgtcagacga ccaatcaaaa
    81901 actccattga gatgtagtta acatgtcgct gattcgccac cggtttggcg aatggctgag
    81961 cacgcagcat ttcggccagt gcttcactca ctgccagcca ccactggcga ggagtcattt
    82021 cagccgcaga atttaagcca taacgctgcc actgacgtga aagcgcttcc tgaaattgct
    82081 tatcgttaaa aataggttgt gacataggag ttccactttt cttagatttt caacacaacg
    82141 ttatcgctag tttgccaggc tcgatgttga ccttcctcat cctgcggggg attaggcagg
    82201 gaggagttgc ggggatgagc aaggaaatgt gatctcgacc acttaaagct agtgcaaacc
    82261 acaggattag cagcaaatca atgccataca gcgcagaaaa tctgtatcta agtgcaaaaa
    82321 atggccgttg cgtattttca aaaagcggaa ggtaactcta taaattaagt aaaggagtga
    82381 aacagtttca caagtaaaat atccagtgtg ctccatctca ttcttaatag atttattaag
    82441 atcatctttt tagatggcac tttcatcagg aatgaagaag aaacccttgc ttaaatgaat
    82501 ctgat
//
LOCUS       DAJTXY010000005        68898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTXY010000005 DAJTXY010000000
VERSION     DAJTXY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.005"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..188
                     /locus_tag="OO566_000419"
     CDS             <1..188
                     /locus_tag="OO566_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6603832.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
     gene            complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="OO566_000420"
     CDS             complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="OO566_000420"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6603833.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1156..3192
                     /gene="lpoA"
                     /locus_tag="OO566_000421"
     CDS             1156..3192
                     /gene="lpoA"
                     /locus_tag="OO566_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6603834.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLALQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3150..3545
                     /locus_tag="OO566_000422"
     CDS             3150..3545
                     /locus_tag="OO566_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6603835.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3565..4155
                     /gene="diaA"
                     /locus_tag="OO566_000423"
     CDS             3565..4155
                     /gene="diaA"
                     /locus_tag="OO566_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6603836.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4165..4740
                     /gene="yraP"
                     /locus_tag="OO566_000424"
     CDS             4165..4740
                     /gene="yraP"
                     /locus_tag="OO566_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6603837.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4854..5894)
                     /locus_tag="OO566_000425"
     CDS             complement(4854..5894)
                     /locus_tag="OO566_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6603838.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLPGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5967..6602)
                     /locus_tag="OO566_000426"
     CDS             complement(5967..6602)
                     /locus_tag="OO566_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6603839.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6730..7248
                     /gene="yhbO"
                     /locus_tag="OO566_000427"
     CDS             6730..7248
                     /gene="yhbO"
                     /locus_tag="OO566_000427"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6603840.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7228..7671)
                     /locus_tag="OO566_000428"
     CDS             complement(7228..7671)
                     /locus_tag="OO566_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6603841.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7722..8024
                     /gene="yhbQ"
                     /locus_tag="OO566_000429"
     CDS             7722..8024
                     /gene="yhbQ"
                     /locus_tag="OO566_000429"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6603842.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8011..8514)
                     /locus_tag="OO566_000430"
     CDS             complement(8011..8514)
                     /locus_tag="OO566_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6603843.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8508..9032)
                     /locus_tag="OO566_000431"
     CDS             complement(8508..9032)
                     /locus_tag="OO566_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6603844.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVCDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9241..10236
                     /locus_tag="OO566_000432"
     CDS             9241..10236
                     /locus_tag="OO566_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6603845.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10245..11123
                     /locus_tag="OO566_000433"
     CDS             10245..11123
                     /locus_tag="OO566_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6603846.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11204..12211
                     /locus_tag="OO566_000434"
     CDS             11204..12211
                     /locus_tag="OO566_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6603847.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="OO566_000435"
     CDS             complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="OO566_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6603848.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13727..15616)
                     /gene="deaD"
                     /locus_tag="OO566_000436"
     CDS             complement(13727..15616)
                     /gene="deaD"
                     /locus_tag="OO566_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6603849.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="OO566_000437"
     CDS             complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="OO566_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6603850.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="OO566_000438"
     CDS             complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="OO566_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6603851.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEHTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="OO566_000439"
     CDS             complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="OO566_000439"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6603852.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="OO566_000440"
     CDS             complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="OO566_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6603853.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19589..20533)
                     /gene="truB"
                     /locus_tag="OO566_000441"
     CDS             complement(19589..20533)
                     /gene="truB"
                     /locus_tag="OO566_000441"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6603854.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="OO566_000442"
     CDS             complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="OO566_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6603855.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21098..23746)
                     /gene="infB"
                     /locus_tag="OO566_000443"
     CDS             complement(21098..23746)
                     /gene="infB"
                     /locus_tag="OO566_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6603856.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="OO566_000444"
     CDS             complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="OO566_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6603857.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="OO566_000445"
     CDS             complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="OO566_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6603858.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25945..26021)
                     /locus_tag="OO566_000446"
     tRNA            complement(25945..26021)
                     /locus_tag="OO566_000446"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25985..25987),aa:Met,seq:cat)
     gene            26369..27712
                     /gene="argG"
                     /locus_tag="OO566_000447"
     CDS             26369..27712
                     /gene="argG"
                     /locus_tag="OO566_000447"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6603859.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27720..29345)
                     /locus_tag="OO566_000448"
                     /pseudo
     CDS             complement(27720..29345)
                     /locus_tag="OO566_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(29804..29890)
                     /locus_tag="OO566_000449"
     tRNA            complement(29804..29890)
                     /locus_tag="OO566_000449"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29854..29856),aa:Leu,seq:gag)
     gene            complement(29905..30237)
                     /gene="secG"
                     /locus_tag="OO566_000450"
     CDS             complement(29905..30237)
                     /gene="secG"
                     /locus_tag="OO566_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6603860.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30465..31802)
                     /gene="glmM"
                     /locus_tag="OO566_000451"
     CDS             complement(30465..31802)
                     /gene="glmM"
                     /locus_tag="OO566_000451"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6603861.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRVLQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31795..32643)
                     /gene="folP"
                     /locus_tag="OO566_000452"
     CDS             complement(31795..32643)
                     /gene="folP"
                     /locus_tag="OO566_000452"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6603862.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32733..34667)
                     /gene="ftsH"
                     /locus_tag="OO566_000453"
     CDS             complement(32733..34667)
                     /gene="ftsH"
                     /locus_tag="OO566_000453"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6603863.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34767..35396)
                     /gene="rlmE"
                     /locus_tag="OO566_000454"
     CDS             complement(34767..35396)
                     /gene="rlmE"
                     /locus_tag="OO566_000454"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6603864.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35522..35815
                     /gene="yhbY"
                     /locus_tag="OO566_000455"
     CDS             35522..35815
                     /gene="yhbY"
                     /locus_tag="OO566_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6603865.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKKRKISLPR"
     gene            complement(35971..36447)
                     /gene="greA"
                     /locus_tag="OO566_000456"
     CDS             complement(35971..36447)
                     /gene="greA"
                     /locus_tag="OO566_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6603866.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36695..38128
                     /gene="dacB"
                     /locus_tag="OO566_000457"
     CDS             36695..38128
                     /gene="dacB"
                     /locus_tag="OO566_000457"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6603867.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38314..39486)
                     /gene="cgtA"
                     /locus_tag="OO566_000458"
     CDS             complement(38314..39486)
                     /gene="cgtA"
                     /locus_tag="OO566_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6603868.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39502..40467)
                     /locus_tag="OO566_000459"
     CDS             complement(39502..40467)
                     /locus_tag="OO566_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6603869.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40594..40851)
                     /gene="rpmA"
                     /locus_tag="OO566_000460"
     CDS             complement(40594..40851)
                     /gene="rpmA"
                     /locus_tag="OO566_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6603870.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40872..41183)
                     /gene="rplU"
                     /locus_tag="OO566_000461"
     CDS             complement(40872..41183)
                     /gene="rplU"
                     /locus_tag="OO566_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6603871.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41442..42413
                     /gene="ispB"
                     /locus_tag="OO566_000462"
     CDS             41442..42413
                     /gene="ispB"
                     /locus_tag="OO566_000462"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6603872.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42641..42919
                     /gene="sfsB"
                     /locus_tag="OO566_000463"
     CDS             42641..42919
                     /gene="sfsB"
                     /locus_tag="OO566_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6603873.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(42967..44226)
                     /gene="murA"
                     /locus_tag="OO566_000464"
     CDS             complement(42967..44226)
                     /gene="murA"
                     /locus_tag="OO566_000464"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6603874.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44281..44535)
                     /gene="ibaG"
                     /locus_tag="OO566_000465"
     CDS             complement(44281..44535)
                     /gene="ibaG"
                     /locus_tag="OO566_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6603875.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44695..44988)
                     /gene="mlaB"
                     /locus_tag="OO566_000466"
     CDS             complement(44695..44988)
                     /gene="mlaB"
                     /locus_tag="OO566_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6603876.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44988..45623)
                     /gene="mlaC"
                     /locus_tag="OO566_000467"
     CDS             complement(44988..45623)
                     /gene="mlaC"
                     /locus_tag="OO566_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6603877.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45642..46193)
                     /gene="mlaD"
                     /locus_tag="OO566_000468"
     CDS             complement(45642..46193)
                     /gene="mlaD"
                     /locus_tag="OO566_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6603878.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46198..46980)
                     /gene="mlaE"
                     /locus_tag="OO566_000469"
     CDS             complement(46198..46980)
                     /gene="mlaE"
                     /locus_tag="OO566_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6603879.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46988..47797)
                     /gene="mlaF"
                     /locus_tag="OO566_000470"
     CDS             complement(46988..47797)
                     /gene="mlaF"
                     /locus_tag="OO566_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6603880.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48007..48972
                     /locus_tag="OO566_000471"
     CDS             48007..48972
                     /locus_tag="OO566_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6603881.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48986..49972
                     /gene="kdsD"
                     /locus_tag="OO566_000472"
     CDS             48986..49972
                     /gene="kdsD"
                     /locus_tag="OO566_000472"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6603882.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49993..50559
                     /gene="kdsC"
                     /locus_tag="OO566_000473"
     CDS             49993..50559
                     /gene="kdsC"
                     /locus_tag="OO566_000473"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6603883.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50556..51131
                     /gene="lptC"
                     /locus_tag="OO566_000474"
     CDS             50556..51131
                     /gene="lptC"
                     /locus_tag="OO566_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6603884.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51100..51657
                     /gene="lptA"
                     /locus_tag="OO566_000475"
     CDS             51100..51657
                     /gene="lptA"
                     /locus_tag="OO566_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6603885.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51664..52389
                     /gene="lptB"
                     /locus_tag="OO566_000476"
     CDS             51664..52389
                     /gene="lptB"
                     /locus_tag="OO566_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6603886.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52437..53870
                     /gene="rpoN"
                     /locus_tag="OO566_000477"
     CDS             52437..53870
                     /gene="rpoN"
                     /locus_tag="OO566_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6603887.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53893..54180
                     /gene="hpf"
                     /locus_tag="OO566_000478"
     CDS             53893..54180
                     /gene="hpf"
                     /locus_tag="OO566_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6603888.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54298..54789
                     /gene="ptsN"
                     /locus_tag="OO566_000479"
     CDS             54298..54789
                     /gene="ptsN"
                     /locus_tag="OO566_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6603889.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54835..55689
                     /gene="rapZ"
                     /locus_tag="OO566_000480"
     CDS             54835..55689
                     /gene="rapZ"
                     /locus_tag="OO566_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6603890.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55686..55958
                     /gene="npr"
                     /locus_tag="OO566_000481"
     CDS             55686..55958
                     /gene="npr"
                     /locus_tag="OO566_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6603891.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56171..56803
                     /gene="yrbL"
                     /locus_tag="OO566_000482"
     CDS             56171..56803
                     /gene="yrbL"
                     /locus_tag="OO566_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6603892.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56800..57528)
                     /gene="mtgA"
                     /locus_tag="OO566_000483"
     CDS             complement(56800..57528)
                     /gene="mtgA"
                     /locus_tag="OO566_000483"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR6603893.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57525..58178)
                     /gene="elbB"
                     /locus_tag="OO566_000484"
     CDS             complement(57525..58178)
                     /gene="elbB"
                     /locus_tag="OO566_000484"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6603894.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58408..60744)
                     /gene="arcB"
                     /locus_tag="OO566_000485"
     CDS             complement(58408..60744)
                     /gene="arcB"
                     /locus_tag="OO566_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6603895.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEKSTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60840..61769)
                     /locus_tag="OO566_000486"
     CDS             complement(60840..61769)
                     /locus_tag="OO566_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6603896.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62444..66904
                     /gene="gltB"
                     /locus_tag="OO566_000487"
     CDS             62444..66904
                     /gene="gltB"
                     /locus_tag="OO566_000487"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6603897.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPTTTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66917..68335
                     /gene="gltD"
                     /locus_tag="OO566_000488"
     CDS             66917..68335
                     /gene="gltD"
                     /locus_tag="OO566_000488"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6603898.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68519..>68898
                     /locus_tag="OO566_000489"
     CDS             68519..>68898
                     /locus_tag="OO566_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6603899.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSA"
BASE COUNT        16829 a        18733 c        17124 g        16212 t
ORIGIN      
        1 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
       61 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
      121 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
      181 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
      241 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
      301 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
      361 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
      421 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
      481 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
      541 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aatcagcgtg
      601 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
      661 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
      721 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
      781 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
      841 ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
      901 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aaccggtgtg acgagtatcc
      961 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     1021 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     1081 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     1141 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     1201 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     1261 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     1321 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     1381 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     1441 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     1501 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     1561 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     1621 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     1681 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     1741 gccgacgaaa atattctgca aggctggctg gctctgcagc gcgtctggtt tgataaccgt
     1801 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     1861 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     1921 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     1981 attcagcaag gctttgaagc cgcgaaaaat atcggcactc agccagtggc ggctcaggta
     2041 gctgccgcac ctgccgcaga cgtagcagaa caacctcagc cgcaaactgc ggatggcgtt
     2101 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg atcagcctgc ggcccagccg
     2161 gtgcctgtaa gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc
     2221 gcagagctga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     2281 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     2341 ctgaagagca acaccccgct gagcgtactg gcactgaacc agccggagaa tatcgaaaac
     2401 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     2461 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag tgcattgggc
     2521 gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg
     2581 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt
     2641 gctttaacgg gtagcccgat tactcccaga gcgacaaccg actccggcat gacgaccaac
     2701 aatccaacgc tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc
     2761 gatgcggtct acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc
     2821 atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc
     2881 accgcaggcc cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg
     2941 ctggcaggcg gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa caacgattat
     3001 tcgctggctc gcatgtatgc gatgggcgtt gatgcctggt cgttggcaaa tcatttctca
     3061 caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagtct gacggctaac
     3121 ccggattgcg tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc
     3181 cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc
     3241 tggaaggcaa aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg
     3301 atctgataat gcgtgaaggc ctgaccaccg tttttgtcga ggtacgctat cgccgctctg
     3361 cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga
     3421 ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg
     3481 atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact
     3541 cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc
     3601 attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg
     3661 acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc
     3721 gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc
     3781 agcttacctg ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat
     3841 cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta
     3901 ttgttagcca tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc
     3961 gttacgcgtg atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt
     4021 ttgttagggc cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag
     4081 gaaatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct
     4141 caccaggatg attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc
     4201 gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc
     4261 aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg
     4321 cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga aagaaacccg tattaatgtc
     4381 acggcctatc agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct
     4441 cgcgccaaac agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt
     4501 cagggccagc cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg
     4561 cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa
     4621 aacggtgaag tgttcctgat ggggctggta actgaacgtg aagcgaaagc ggcggcagat
     4681 attgccagcc gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag
     4741 cagccccttg taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca
     4801 aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac
     4861 aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat
     4921 aaggctttcg ccgggaacgc tttgcgcagc ataatcagtg acggcaaact cactgccggg
     4981 agcgtcatca acaatgccag cgccggagcg gttcccatac ctgccagcat catcgtttgt
     5041 acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc
     5101 accacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcgt
     5161 gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagtgtc
     5221 catagcgccc tgccccagcg gctaaaaaaa ccgctctgtg cttccggtat gtcaatttcg
     5281 accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc
     5341 aacaccatca ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac
     5401 cccataaaca ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca
     5461 ccgcccatcg atacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta
     5521 cacatcatgc ccggcagcga aaacagcgtt cccggcagcg tgccgcgaaa gcgcgattgc
     5581 ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc
     5641 acccccagca ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc
     5701 gcttgccacg gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg
     5761 gctgcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg
     5821 acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa
     5881 gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta
     5941 cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt
     6001 aaaatcgtca cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg
     6061 tctctcgcat caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc
     6121 gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc
     6181 aatttcggcc agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag
     6241 aaaaacggcg agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc
     6301 agtcgccgcc cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc
     6361 tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac aaaatacaat gtcgatgggt
     6421 tcggtgacct gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc
     6481 atatcgccca acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc
     6541 aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc
     6601 atcttcgctc ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccagtcttaa
     6661 tagaccatga ggtaattatc cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc
     6721 ggaggaagca tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa
     6781 tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac tattgaaaaa
     6841 caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc
     6901 atcgatgaag tgacgccagc ggagtttgat gccctgttgc taccgggtgg tcattcaccg
     6961 gattatctgc gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc
     7021 aaacctgttt tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg
     7081 gggcgcaaac tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgtggaa
     7141 ttttacgatc aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac
     7201 gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca
     7261 aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat
     7321 ttcccacacc ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg
     7381 cgcgaggtcg ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg
     7441 aattaacgct accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc
     7501 actcatctgc gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc
     7561 atcaaaaaga taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt
     7621 gacaacatgt tgttttgcca gccaacggct gatggcgatg agtgtttcca ttgctgttct
     7681 cttttatact gtgggcctga ccttaacatg cgatgcagac gatgaccccc tggtttcttt
     7741 acttgatccg taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc
     7801 gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga
     7861 cgctggcgtt ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg
     7921 ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag
     7981 aactgttgag cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc
     8041 aaccagaccg gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct
     8101 ttcagtgccc ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg
     8161 actgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga
     8221 atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac
     8281 cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa
     8341 tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa
     8401 gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg
     8461 cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca
     8521 cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc
     8581 agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc
     8641 aggtttttca catacacccc cagctccgta tcgccttcaa tcaccagccg acgctggaag
     8701 aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca
     8761 ctaaaactca catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag
     8821 gtaaaccatt gcaggtcaat atcacacaca tgaatactta accagcggcc ttcaagaaac
     8881 tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc
     8941 tggcgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa
     9001 tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat
     9061 cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat
     9121 tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta
     9181 actaaaatcc cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt
     9241 atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc
     9301 gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt
     9361 aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa
     9421 cttcacatct cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc
     9481 gtggatatgg cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg
     9541 gagtacgccg ccgagcgtta cccgcatatt gaacgtcatg tgtcagtgca ggcttcggcg
     9601 accaatgaag aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg
     9661 ccgcgcgtgt tgtcgattca tcaggtgaaa cagctggcac gggttactcc tgtcccgctg
     9721 gaagtgtttg ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg
     9781 tatctgacgg gtgagtcgcc caacactgtg ggtgcgtgtt ctccggcccg tttcgtgcgc
     9841 tggcaacaaa cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat
     9901 caggacggcg aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc
     9961 gagcgctatc acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag
    10021 ttaatggctg cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat
    10081 gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa
    10141 aacttcgtac cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa
    10201 accacccttg gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag
    10261 ggccagtgct gtggtactgg ccaaaagaga cgctggaaga tttttatcag caagccgccg
    10321 ccagcagcgc cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc cgggcaacca
    10381 aagttggcga ctgtctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc
    10441 tctccacgct ggcgctggtg caggcatcct ctgaactggg cgaactgaaa cgctatgttg
    10501 agaacggtga gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac
    10561 gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa
    10621 tattgctcaa acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc
    10681 tggtgaatct gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag
    10741 tcctgagcta cggtcatcta ccgctggcct actccgcccg ctgctttacc gcgcgttcgg
    10801 aagaccgccc gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg
    10861 tgctgtcgca ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct
    10921 acgtttacaa cctcggtaac gagctggcat ccatgcaggg attggttgat gtagtccgcc
    10981 tgtcaccgca gggcactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg
    11041 gcgctgtgcc actgccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg
    11101 caggactgga gctgcaagcc taagtaaata gctcactttg ttaacaactt taactattct
    11161 ttaatgcagt attaaagatt aatcggtaac aaagtgagct gttatgactg ataaaaccat
    11221 tgcgttttcg ctactcgatc tggcccccat acccgaaggc tcttcagcgc gggaagcgtt
    11281 ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct
    11341 ggcagaacac cacaatatga ccggcattgc cagtgctgca acatcggtat tgattggcta
    11401 tctggcggcg aataccacca cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca
    11461 ctcaccgttg gtcattgccg aacagttcgg cacactcaat acgctctatc cggggcgaat
    11521 cgatttgggg ctgggtcgtg ctccgggtag tgaccaacgg acaatgatgg cgctacgtcg
    11581 tcatatgagc ggcgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt
    11641 tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc
    11701 cgtgtggttg ttaggctcca gcctttacag tgcacaactg gcggcgcagc ttggtctgcc
    11761 atttgcgttt gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg
    11821 cagtaacttc aaaccgtcgg ctcggctgga aaaaccatac gcgatggtgt gcatcaatat
    11881 tatcgccgcc gacagcaacc gcgacgctga atttctgttt acctcaatgc agcaagcctt
    11941 tgtgaagctg cgccgtggcg aaaccgggca actgccgccg ccgattcaaa atatggatca
    12001 gttctggtca ccgtctgagc agtatgacgt gcagcaggcg ctgagtatgt cgttggtagg
    12061 cgataaagcg aaagtgcgtc atggtttgca gtcgatcctg cgcgaaaccg acgctgatga
    12121 gattatggtt aacgggcaga ttttcgacca ccaggcgcgc ctgcattcgt ttgagctggc
    12181 gatggatgtt aaggaagagt tgttgggata gtgtgtctta acgcgggaag ccttatccga
    12241 gctggcaacg ctgtcctacg taggcctgat aagcgaagcg catcaggcat tgtgtaggca
    12301 gcagaaatgt cggataaggc accgctgatt actgatacac cggcagtaaa ttaaagctcg
    12361 ataaaatatg caccagtgcg ttgccgacgc caaacaccag aatcagcgca atcatcggct
    12421 tgccacccca gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg cgggccagca
    12481 gtgccggaac aattgccgcc cagatggtag ccgctaaacc agcataacca atggcataca
    12541 ggaatccgtt cgggaacagc agaccaccca caacaggcgg ggcaaaggtc agcaatgccg
    12601 ttttcaagcg gcccacagcc gagtcgtcga aaccaaacag atctgccaga tagtcaaaca
    12661 aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca
    12721 gaaggtccag gctacggctg ttcagcacgc cgcttaatgc ttgtaccagc acatcaatat
    12781 taccgccctt ctcggcgatg ccgataaact ccggacgagg gatgttgccc attgtcgcca
    12841 gcaaccagat ggtatacagc gccagtgcca tcagcgtacc gtacaccagg catttcacga
    12901 tggttttcgg atctttgccg taatacttca tcaggcttgg cacgttaccg tgataaccaa
    12961 acgacgccaa gcagaacggc agggtcatca gcaaatacgg cgcataagat gcattgcttt
    13021 cggcaacgtt gaacaatgtc gcaggctgca catgccccag caggctaccg aaggtgagga
    13081 agaaggtaat gactttcgcc cccagcacaa tcgccgtcat gcggctgacg gctttagtac
    13141 tcaaccacac cacaaacgct accagcagtg caaaaccaaa acccgccgcc cgtgccggga
    13201 cgtttagcga catctctgcg aaggtgtgat gcagaatcga accactggca gaaatatagg
    13261 cataggtcag gatatagagc acaaaggcaa tggaaatgcc gttgaccacg ttccagcctt
    13321 tgcccagcaa atctttggtg atggtgtcaa aactcgaacc gattcggtaa ttcaggttag
    13381 cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgctgcca
    13441 ttgaccagaa aaaccacgcc ccggacatga ccactggcag agaaaacatc cctgcgccaa
    13501 taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg
    13561 ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgcat
    13621 tgcactgtac cagtacatga gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg
    13681 ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac gcatcaccac
    13741 cgaaacgacg acgaccggta gagtcatcac gacgcggtgc gcggccttca cgacgttcgc
    13801 cgctaaaacg acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac
    13861 cgccttcacg acgctcgcca ccgaaaccac gaccaccgcc acgaagctca ccgccagtat
    13921 gcggctgtgc atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg
    13981 taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag
    14041 caaacagctt gatgttaccg atgtaacggc tgctgatgtc gccttcgtta gcaatcgcgc
    14101 caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca
    14161 tatcgccaac atcacgacgt tcacgacgcg gacgatcttc acggtcgcca cgcgggccac
    14221 ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca
    14281 tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgccg
    14341 cagccagagt ttcgagatcc agctcttcac cttctgcagt cggctgaatt ttgctcagca
    14401 gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt
    14461 tttccagacg gcgtttgccc agcagttctg cgttcggcag ttctacttcc ggaatagtca
    14521 gcttcatagt acgttcaatg ttgcgcagca gacgacgctc gcggttctca acgaacagca
    14581 gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat acggtgaacg taagactcag
    14641 aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc aggccacggg
    14701 ccgcaacgtc ggtcgcaatc aggatgtcca gacgaccgtc tttaaggcgt tccagtgtct
    14761 gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctattgtag ccgttacgct
    14821 caagagcttc agccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat
    14881 caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccacacag
    14941 tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaata cgcacttcct
    15001 gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga
    15061 acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga
    15121 agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt
    15181 ccagagtgcc acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg
    15241 gcccctgacg cagggcgcgt aattgcacgt cataacgctg gccgccgtac agggcaacca
    15301 catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca
    15361 gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct
    15421 gcagcagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa
    15481 cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt
    15541 aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg
    15601 tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac
    15661 tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt
    15721 attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc
    15781 tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag
    15841 gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac
    15901 cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc
    15961 taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatcgg ttgcgtccgc
    16021 cttgagcctt tccattaacg tgtgttcgct aatgttgccc aggtagaact cgacaatgtt
    16081 ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg
    16141 cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg
    16201 atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata
    16261 taatgcaatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga
    16321 atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta
    16381 attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc
    16441 taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg
    16501 ttcgtcatcg gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc
    16561 ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt
    16621 actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat
    16681 ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt
    16741 cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg
    16801 ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat
    16861 gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc
    16921 catctgcagg taatcggtca ctttctcaac gcgtttgtca gcgatttgag agatgtgtac
    16981 cagaccttct ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac
    17041 tttaccagtg tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat
    17101 agcatgtttc gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt catcttcgat
    17161 ttcgatggtg gtgccagttt cttcggtcag ggcacggata acagagccgc ctttaccgat
    17221 aacgtctttg atcttgtccg ggttgatctt gatggtatgg atacgcggtg cgaactcaga
    17281 gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc
    17341 acctttagcc tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat
    17401 atccatctgc aatgcagaga taccctcgcg ggaacccgca actttgaagt ccatatcgcc
    17461 caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac
    17521 cagacccatt gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc
    17581 cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcag tgatttcaga
    17641 cacaacacgt acggtgtacg ggaatttgtc catatccggc atgactgcca gcacgccgcg
    17701 cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc
    17761 gcctacggag tacggaggga agttgtagtg gaacaggaag gtatcggtac gttcgcccat
    17821 cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg
    17881 cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac
    17941 atccagacca cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac
    18001 gcggctacga acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc
    18061 cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca gatttgatga catcaacctg
    18121 cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag
    18181 tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg
    18241 tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg
    18301 accgaacact actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac
    18361 catcagtaca gcggcttcag taccggcaac aaccagatcc agtttgctct ctttcaactc
    18421 gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacgcgag cagcaccaat
    18481 cggaccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac
    18541 gatatccggg ttaacttgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt
    18601 gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg cgatcagggt
    18661 ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc
    18721 gtaggtacgc tcctgatagt taacggtcag tgggaagaag tcctgacctg gtttggcttt
    18781 tttctggcca acaacggtaa caaataccgc ggtgtcatcc atgctaacca taacagcggc
    18841 agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa
    18901 tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac
    18961 ggcactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc
    19021 agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg aagatcattc
    19081 atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac
    19141 tcaggcccct ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag
    19201 ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga
    19261 aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc
    19321 ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcagtag aaccggtgtc
    19381 gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat
    19441 tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt
    19501 gtacgttacg caaagtgtta aacaatttac gcgacgttaa gcggcagtat tctactcgta
    19561 gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag
    19621 gcgcaacgcg gccttcatcg tcaatttcac ccataccgat aaatttgccg ttctcacctt
    19681 ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaactggg ttaccatttt
    19741 tgaagtaaac agaagacgtt aacggaagat tcaccaccgg gtagtccgaa gctggactgt
    19801 ccattggcat cagtaatgga tcaagtaact ccgcagccgg aatatcctgc tgttcggctt
    19861 gttcgaccag ttcacgcaga tgttccaggg tcacaatccg ttcaaccgga tatttactta
    19921 ccgccagacg acgcaggtaa ataacatgcg cgccacaacc gagtttttca cccaggtcat
    19981 caatgatggt gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc
    20041 cttcgtggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca cgcggtactt
    20101 caatgccctg acgcgcatat tcgtacagtt ttttaccctg atatttcagt gctgaataca
    20161 tcgaagggat ctgttcgata tcgccacgga aagtatccag cgccgccgcc agttgctctg
    20221 cgctaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac
    20281 gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga tactgggaaa
    20341 acttcgtcgc ttccccgagg caaattggca acatgccagt tgccagcggg tccagcgcac
    20401 cggtatgccc ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcattgc
    20461 tggacatacc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac
    20521 gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg
    20581 tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta
    20641 gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat
    20701 gaaaccagaa gcttcttgca acgctttgat gcccgcttta accgcgtctt catctttgtc
    20761 gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga
    20821 aacggtggtc atcatgccca ggcgaggatc tttaatttca cgctgcagga tgagagcgat
    20881 ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa
    20941 ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc
    21001 tgttcacgtt ggcctgataa gacgcgccag cgtcacatca ggcaatccat gccggatgcg
    21061 gcgtaaacgc cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggatct
    21121 cgatgatttc gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc
    21181 cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg
    21241 actccagctc gccttcgtag ataaccacgt tgtcacgcag aacgcggatc gggttgtgac
    21301 gtttaaccac accttcggta accatacagc ctgcgatggc accaaatttc ggcgatttga
    21361 acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga gacagcatac
    21421 cgctcatcgc cgctttcact tcgtcaatca ggttatagat gacggagtag taacgcagat
    21481 ccaggctttc cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttgaagccaa
    21541 ccaggatggc gttggacgcc gcagccaggg tggcgtcggt ttcggtgata ccacctacgc
    21601 cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga
    21661 tcgcttccac agaaccctgt acgtctgcct tcaggacgat gttcacttcg tgaacttcgc
    21721 cttcggtcat gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa
    21781 cttcgcggaa tttaccctga cgatacagtg caacttcacg cgctttcttc tcgtcacgta
    21841 caacggtaac ttcatcaccc gcagccggta cgccggacag gccgaggatt tccaccggaa
    21901 tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcatcgca cgaacacgac
    21961 cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa
    22021 cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca
    22081 tacctttacg taccgctttc agctccagaa cttccgcctg cagcaggata gcgtccagca
    22141 gttcatcgat accagtaccc gctttcgcag acacatgtac gaactggctt tcaccgcccc
    22201 actcttccgg caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcggctt
    22261 ctggtttatc gatcttgttc actgcaacga ccaccggaac ctgcgccgct ttcgcgtgct
    22321 ggattgcttc gatcgtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga
    22381 tgtccgttgc ctgcgcacca cgagcacgca ttgaagtaaa cgcggcgtgc cccggggtgt
    22441 ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgagtaa
    22501 tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt
    22561 taccgtggtc aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgcagcac
    22621 ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc aggataactt
    22681 tatggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg
    22741 ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct
    22801 tgttcgccag ttcgccaacg gtgatagttt cgccgatcac aacgtcacgg ttaacggcct
    22861 gagcaggctt ctggaagcct tgctgcagcg aagaaccttt acgttttccg cctttaccgc
    22921 cacgtactgc tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt
    22981 tcttcggacg cgctgctttc gcgttacgac cacggccacg gccgccttcg acttcacgat
    23041 cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa tcgctggaat
    23101 cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt
    23161 cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttca gctttacgct
    23221 tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat
    23281 cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag
    23341 cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag
    23401 ccgattcttc tgcctcacga cgggcttgct cttccgctgc aaggcgttca gcctcttgcg
    23461 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    23521 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    23581 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    23641 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    23701 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    23761 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    23821 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    23881 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    23941 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    24001 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    24061 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    24121 tcgagaaacc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    24181 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    24241 tcattacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    24301 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    24361 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    24421 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    24481 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg
    24541 ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc cgctgcttta atttcaatca
    24601 cttcttcgcc gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    24661 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac
    24721 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    24781 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    24841 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    24901 cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa
    24961 cagactcaat ctgatcttca acgtaatcgc ccaagttcag gctttcatct tcataacgtg
    25021 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    25081 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac acggacgtcg atctcttgct
    25141 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    25201 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    25261 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    25321 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    25381 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    25441 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    25501 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    25561 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    25621 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgcctacc agttcaaaac
    25681 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    25741 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    25801 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    25861 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    25921 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    25981 ggttatgagc ccgacgagct accagactgc tccaccccgc gcctgaaacg tggcaaattc
    26041 tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    26101 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaatcac
    26161 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttccc ctgctctccc
    26221 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    26281 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    26341 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    26401 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    26461 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctaggccagc cagacgaaga
    26521 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    26581 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    26641 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    26701 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    26761 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    26821 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    26881 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    26941 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    27001 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    27061 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggttg cgctgaacgg
    27121 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    27181 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    27241 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    27301 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    27361 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    27421 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    27481 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    27541 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    27601 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tttcctcctc
    27661 tgccacttca ggcctgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    27721 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    27781 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    27841 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    27901 ataaggtaat tattgtaagc tgtcgaaaag atattgtttt ccgttgtgcg atccacgcca
    27961 tccccaagaa cggggctata acagataaac atcgggacat gatatgcctg ctggctagcc
    28021 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    28081 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaaactg acctagcaaa
    28141 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    28201 acgcgctgga ttgcggatag gcgctacaag ccggttcatg gcttccattt aaatgaagaa
    28261 caatcagttt cttttgctgc gtattttgct gtaatgcttg cgataaatgc ggcaacaaca
    28321 attcatcaaa tcctctgaca taaactgttt ccatggcgcg catggcgata ctggtaactg
    28381 ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca gaaagtctga aatcctgctt
    28441 gattagccat attaataatg ttgtcggggt aattatgaat gtcatgactc aaaacagaat
    28501 cagcagttaa agaaagggga accgacagcg cagtgtaagg tgcgccgctt attgcttgat
    28561 taaacagttt gatctgtttt ctttgtgctt caacttgcgg tgtcgtagag cgtgtatatc
    28621 catacaaaga catattgtcg acacgtacag actcccccac aatcaacacg taggtatcaa
    28681 tacttgtatc cctgactgat aattgaaaat acggcaccgt gtttgcaatt gagagtaatc
    28741 tttgatgctc tttcgctgct aaagcaaaat agttgagatt gaaaaacggc gtataggtag
    28801 caaatcacga cgctagtata tatggactga acgctttttt attttttgca tctttataag
    28861 caaattgaca agcggaaaat aaactgcccg aaatgacaat cagcaataat attcctgtca
    28921 ctttttttgt cggcaaggaa acatcatatt ttattattac tgccaaaaaa aaaagggata
    28981 aaaaagcaaa agcacataga taagggatat acatccccag cattttcaca acttcatccg
    29041 gatcactctg cagcacacta atcgcaaatc catcattgaa agttgtacca aaggtacacc
    29101 atgaatagag gctaatactc atgtctgccg cagtcagtaa cacaaatggt gcggcaacaa
    29161 gcaggcgaag cggaagggat gatatacgtt taacaaccag gatggtcagg acgaagaaca
    29221 gcaatcgctg aaccatatgc gctccggaag aggcgactaa gttcgtaata ccaaaaacaa
    29281 taacacaaag atacaacaac caatgagatt taaaatcttc tagcaaattt gttgaatact
    29341 gtcatccaga aatccaagtt cacaaccaat ataaacaatt atcaacaatg ttcaatttta
    29401 tatcagacat gtttttcaac tctgaaccat atcagaccga aacactgtta gttttttctt
    29461 tttaaatgat gcgaccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa
    29521 acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
    29581 aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg
    29641 tcagcagtag ggaaaagttt ataactaagg cgtaccggca ccatcgtttc aaggtaccag
    29701 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcatc agaccaggac
    29761 caaaacgaaa agagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
    29821 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
    29881 ccacctcggc acggatacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
    29941 agctggctga gtttgttcgg ttttcgccgg tgcactcaga ttttcccatt cgctacctct
    30001 attggttttg ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc
    30061 cagcagcgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
    30121 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
    30181 cagaccaaca aggccaattg ccacaataag gaaaactact aaaagagctt cgtacataat
    30241 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
    30301 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
    30361 aattttatcg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgccgaga
    30421 aaaaaggcga caatcgccgc ctttttagcc agttatctaa cgctttaaac ggcttttact
    30481 gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc
    30541 acgcgaatta acggttcggt gccggattta cgcagcaaca cgcgtccacg gttgcccagc
    30601 gcagcttcaa cctctgcggt cacggcttta actgactcat gctcaagcgg atcgccgcta
    30661 cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca ttttcatgcc gctgcaaagg
    30721 tcgtgcaggc tcatatggtt acgtgccatc gccgccagca cctgcaagcc agcaacgatg
    30781 ccgtcaccgg tagtggtttt atccagcagg atcacatgac cggaattctc tgcaccgata
    30841 cgccagcctt tctcctgcat tttttccagt acgtagcggt cacccacttt cgcgcgcgca
    30901 aatggaattc ctaactgttt cagcgccagt tcaagcccca tgttgctcat caatgtaccc
    30961 acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
    31021 tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccgtcgccg
    31081 tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag cacgcgaacg
    31141 tcggtagccc ccacttcggc attgatgttt acaccgtttg gctcacaacc gatagcgata
    31201 acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca
    31261 caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa
    31321 aactcgatat agcgacccgc ggcatcaacg atacggctgg ctttacccag ttctgccgaa
    31381 tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc
    31441 agtttagtgc cgtcgataga gaagaattta atcccattat cgtagaacgg gttatgcgat
    31501 gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
    31561 gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt
    31621 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag
    31681 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggtgtg
    31741 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
    31801 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
    31861 ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca
    31921 ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga
    31981 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
    32041 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
    32101 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt
    32161 cacttctgca aagacatcgt catacttcgg agcttcctgc atggtttttg gatttccctg
    32221 catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc
    32281 ggaaagggag cggatatcat taataatgtg agcgccaact ttcgctgact cacggatgac
    32341 ttctggtttg gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
    32401 cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc ctgggcgcgt
    32461 ggactcgcca ccaacgtcaa tgatcgtcgc gccagcattg atcatcagat tcgcatgttt
    32521 caccgcatct atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt
    32581 gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt
    32641 catgatgtta tccctggtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt
    32701 tcggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg
    32761 gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca
    32821 ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg
    32881 cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca
    32941 tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag
    33001 ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata
    33061 tgtttcgctt tcgctacgct acggccgagg aatacttcac cttcttcttc cgcgtacagc
    33121 agtggaccca atttctcaga gaagccccac tgagtcacca tgttacgtgc caggttggtc
    33181 gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccgta gatgatctct
    33241 tctgccagac gaccaccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg
    33301 ctgattgcgt cgccctcagg caagaagaaa gtcacaccca gcgcacgacc gcgtgggata
    33361 atcgtcactt tatgcaccgg atcgtgttcc ggcaccaggc gaccgataat cgcatgaccc
    33421 gcttcgtggt aagccgttga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc
    33481 gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg
    33541 ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag
    33601 aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggtgccaa tggtacgcga
    33661 cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat ctggcaagcc gaccacaacc
    33721 tggcggtcga aacggccagg acgcaacagg gccgggtcga gaacgtccgg acggttagtc
    33781 gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg
    33841 ttcagagtct gttcacgttc atcgtgacca ccgcccagac cagcgccacg ctggcggcct
    33901 acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac
    33961 atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag
    34021 atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcaa cgttttaccg
    34081 gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag
    34141 cggctcggct cgcgcagata ctctaccagt tcagcaactt cttcttttgc ttcgtcgcag
    34201 cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc
    34261 ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc
    34321 cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt
    34381 ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtaat
    34441 ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg
    34501 atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag
    34561 gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac
    34621 atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga
    34681 ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt
    34741 tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact
    34801 tctcgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag
    34861 cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg
    34921 ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca
    34981 cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc
    35041 tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa
    35101 tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg
    35161 atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca
    35221 ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca
    35281 agtttaaacc aggcacggga acgtaatccc tttttctgtg cctgttgaac atatttatcg
    35341 ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa
    35401 ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt
    35461 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
    35521 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
    35581 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
    35641 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaacaaaaac
    35701 cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
    35761 aacgctggtg ctttatcgcc caactaaaaa acgtaaaatc tcgctgccac gctaagatta
    35821 tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa
    35881 aatgccacgc tctgttcgtt gataaaaggc cgcagagcgg cctttttcct ttctttacaa
    35941 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa
    36001 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
    36061 gccacgagca ataggcgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
    36121 gccaacgatg cgataagtct gttcttcgtc agaatccaga ttcagcaccg ttacggtagc
    36181 accaaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgtttga
    36241 cagcttggct tcgatgtctt tgatacggcc ttcgcagaag ccctgctgtt cacgagctgc
    36301 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
    36361 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
    36421 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
    36481 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
    36541 caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatggtc
    36601 attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
    36661 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
    36721 attgaccagc tgtatagcgt tcagtgttca ggccgcgaat gttgatgagt acgttactca
    36781 actccccgca ggtgccaacc ttgccctgat ggtgcaaaaa gtcggtgcgt cggcccccgc
    36841 tattgattac cacagtcagc agatggcgct gcccgccagt acccagaaag tgattactgc
    36901 gctggcggcg ttgattcaac tcggccccga ttttcgtttc accacgacgc ttgaaaccaa
    36961 aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc
    37021 gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa
    37081 ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc
    37141 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgctg ccgccatagt
    37201 tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aagcctggtg atatggcttt
    37261 tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg
    37321 tggttctgcc gaagcgcaat actgcgaact ggatgtggtg ccaggcgacc tgaaccgctt
    37381 tacgctgacg ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttggcgtgca
    37441 ggatggagcc agctatgccg gtgcaattct gaaagatgag ttaaaacagg cgggtatcac
    37501 ctggagcgga acactgctgc gccagactca ggttaacgaa cctggaacgg tagttgccag
    37561 taaacagtcg gccccgctgc acgatctgct taagattatg ctgaaaaagt cggacaacat
    37621 gatcgccgat acggttttcc gcatgatcgg ccatgcgcgc ttcaatgtgc ctggaacatg
    37681 gcgggctggg tcggacgccg tgcgtcagat cctgcgccag caagccggtg tcgatattgg
    37741 taacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac
    37801 catgatgcag gtgctgcaat acattgccca acacgacaat gaacttaact ttatctccat
    37861 gctgccgctt gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
    37921 cgtggatggc aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
    37981 attcattacc acagcgagcg ggcaacgaat ggcatttgta caatatcttt ctggctatgc
    38041 agtaaaaccc gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt
    38101 gtacaaagat atttatcaga acaattagtc aaaaagaaac cccggcgcat ggccggggtt
    38161 tcagattatt gacaaagtgc gctttgttta tgccggatgc ggcgtgaacg ccttatccgg
    38221 cctacaaaat cgtgcaaatt caatatattg caatcatctt gtaggcctga taagtgtagc
    38281 gcatcaggct tatttggcat tggtcattag tgattaacgc ttgtaaatga actcaacgcc
    38341 ttcttcgtcg tcttcgtccc agtcgtcatc ccagtcttca tcatcctctt cagcaatctc
    38401 ttcaagctgc tgacggtgat aatcatccca catgaattcg actttctctg gctgtttcgc
    38461 ttcttcagcc tgcacgaccg ggttttcaat gataaaggtc atcacatccc agcagagatc
    38521 tttcacgccc agtccgctcg ccgcagagat cagataatat ttatcttccc agcccagcgc
    38581 ttcagcgatc gctttcgctt tctcttcggc ttctgcctta tccagcagat cgatcttgtt
    38641 gaagactaac caacgcggtt tcgccgccag atcctggctg tatttttcca gctcgctgat
    38701 aataatacgc gcgttttcaa ccggatcggt gccgtcaatc ggatcgatat cgatgaggtg
    38761 caacaggacg cggcaacgtt ccaggtgctt caggaagcga atgcccagac ctgcgccttc
    38821 cgcagcgcct tcaatcagtc ctggaatatc ggcaacaacg aagctctttt cgttgtccat
    38881 tcgtaccaca cccagacttg gcaccagagt ggtaaacgga taatccgcca ctttcggttt
    38941 agccgccgat accgcacgaa taaaggtcga tttacccgcg tttggcatcc ccaacatacc
    39001 gacgtcagcc agcagcatca gctccagcag cagctcacgc ttatcgcccg gcgtaccgtt
    39061 agttttctgc cgtggtgtac ggttaactga cgatttgaaa cgggtattgc ccagaccgtg
    39121 ccagccgccc ttagcaacca gcagacgctg accgtgttta gtcatatcac ccatggtttc
    39181 accagtaccc tggtcgatta cacgcgtacc taccggcact ttaatcgtca cgtctttacc
    39241 gcgcttaccg gtacaatcgc ggcttgcgcc gttctgaccg cgctctgcac ggaaagattt
    39301 ttcaaaacga taatcgataa gcgtgttcag gttctcgtcg gcttccatcc atacgtcacc
    39361 accatcaccg ccgtcgccgc catccgggcc gcctttcgga atatactttt cgcggcggaa
    39421 gctcacgcaa ccattaccgc catcacctgc aacgaccaga atcgatgctt catcaacaaa
    39481 cttcatttta ttctccgtaa atcattcgcc tgcgcggggt tgcgatacca ccgttgtatg
    39541 cttacgtaat ccgccccaaa tacgatgacc aatggcggaa tacatcgcgc ccgcaaccac
    39601 gacaaacgca ccgagataac ctaaaaggtt taacatcggt ctggcgaaga aatcgggcca
    39661 ggccagtgat aaaagatctg aaaaaaacag cgtaaacagt ggggtgagcg tgatgatcgc
    39721 gctcacctga gctgcctgcc agcgagccat cgcttccgcc aaggcgccat atcctaccaa
    39781 ggtattcagt ccgcaaaaaa ttaaacatgc gagctgccag tggctaagct gcgctatcac
    39841 tccaggcttt gccagaggga agagcgcgat tgtacataaa gtgtacagta aaaacaggat
    39901 ctgcggtgag gccagccgac gcaataaaac cttttgcgcc acgccataac tcacccaaac
    39961 cgtcgccgca ccgaccccaa agataactcc ccaggtgtaa tcggtgagct ttgtaaatat
    40021 ctcgaccaga ctggtgttaa aaaacatcac cagaccgctc aggagcatca atgcccctac
    40081 aacctgagtg ctgcgcattt tctctttcag gataaatacg ctggcaacca tcatgccaac
    40141 tggcgagagt tgcccaatca cctgcgaagc ggtcggactc aggtattgca aggatgagct
    40201 gaacaggatg aagttcccaa acagcccggc ggtcgccact gccaacaaaa tcaaccagcg
    40261 tggcttacga aacacgcgta atggcggcaa cctcttcttc accgcaagaa tggcaccaag
    40321 gccaatactc gccatcaaga aacggtaaaa cacgattgtc ggaggttcca tcacctccag
    40381 cacctgcttc attgcgattg gcaacgcccc ccagcaaatt gctgtggtaa gcgccaaaag
    40441 aatgccaatg cctgcctgct gcttcatacc gtattccctg caaagcgcat ttgcggatta
    40501 acgtcgcacc atgcgcattt ttaccggtta tcgaatgtaa aaagccccgc aacgtgttgc
    40561 ggggctttca tccgttaccg ggacgcgaaa aacttattca gcttcgatgc tgataaattt
    40621 acggtttttc gggcctttaa cttcgaattt cactttaccg tctgctttag caaacagagt
    40681 gtggtcacga ccgcaaccta cgttagcgcc agcgtggaat ttggtaccac gttgacgaac
    40741 gatgatgcta cccgccagaa cggattcgcc accgaaacgc ttaacgccca ggcgtttagc
    40801 ttctgaatcg cgaccgttac gtgtggagcc gccagccttt ttatgtgcca tttgaaatct
    40861 ctcctcaggt cttaggcgct gatgccagta attttcacat cagtgaacca ctgacgatgg
    40921 ccctgctgct tacgatagtg tttacgacga cgaaacttaa cgattttaac tttctcgcca
    40981 cgaccgtgag caacaacttc agctttgatt acgccgccat caacgaaagg aacgccgatt
    41041 ttgacttctt caccgtttgc gatcatcagc acttcagcga actcaacagt ttcgccagtt
    41101 gcgatgtcca gcttttccag gcgaacggtc tgaccttcgc ttactcggtg ttgtttacca
    41161 ccactttgga aaaccgcgta catataaaac tccgcttccg cgcacacctt ttcaatgatt
    41221 caaagtgcgc tataaatatt cacaataggg cgcgaatatt acgcaaaacg cacgcctttg
    41281 acaagtgcta cagtcaatac acgaagaaaa aaaacacaac ttgtacggta acgtttatct
    41341 gtgccatttt ttcagtacaa ttaccctata ttcctaacca taaaccctaa gttgcctttg
    41401 ttcacagtaa ggtaatcggg gcgaaaagcc cggcttttgc gatgaattta gaaaaaatca
    41461 atgagttaac cgcgcaagat atggcgggtg ttaatgcggc aatccttgag cagcttaatt
    41521 ccgacgtcca actgatcaat cagttaggct attacatcgt cagcggcggc ggtaaacgta
    41581 ttcgtccgat gattgctgta ctggctgcac gagctgttgg ctatgaggga aatgcgcatg
    41641 tcaccattgc tgccctgatc gagtttatcc acacggcgac tctgctacac gacgacgttg
    41701 tggatgaatc agatatgcgc aggggtaaag ctaccgccaa cgccgcattt ggcaatgccg
    41761 ccagcgtgct ggtaggcgat tttatttata cccgcgcgtt ccagatgatg accagcctcg
    41821 gttcactcaa agtgctggaa gtcatgtcag aagctgtaaa cgtcatcgca gaaggtgaag
    41881 ttctgcaact gatgaacgtt aacgatccgg acatcactga agaaaactac atgcgcgtta
    41941 tctatagcaa aaccgcgcgt ctgtttgagg ctgccgcgca gtgttccggg attctggctg
    42001 gctgtacgcc ggaggaggag aaaggcctgc aggattatgg gcgctatctc ggcactgctt
    42061 tccagttgat cgacgattta ctcgattaca atgccgatgg cgaacagtta ggtaaaaacg
    42121 tcggcgacga tctgaacgaa ggtaaaccga cgctgccgct gctgcatgcg atgcatcatg
    42181 gcacaccaga acaggcacag atgatccgta ccgccatcga acaaggtaac ggtcgccatc
    42241 ttctggaacc ggttctggaa gcaatgaacg cttgtggatc tcttgaatgg acgcgtcagc
    42301 gcgccgagga agaagcagac aaagccatcg cagcgttaca ggtgctcccg gacacccctt
    42361 ggcgagaagc actcatcggc ctcgcgcaca tcgctgttca acgcgatcgt taatcccctc
    42421 ccttcatccc gcgcagtgcg cgggatgatt ccagtaaatt ccataaaaac actaattcag
    42481 cgctataaca cctgaaaaaa agggcgatga atctttatat attctgaata ttcacactct
    42541 ttacaggaac tttttagcgc aataggccat caggagtata gtgatgcccg acagaagaag
    42601 tgttctgaat gaaagcgact aacttaagga gtgaggaaag atggaaagta atttcattga
    42661 ctggcatccc gctgacatca ttgcaggttt gcgcaagaaa ggaacgtcaa tggcggcgga
    42721 atctcgcaga aatggtttga gttcctcaac gctggcaaac gcattatcgc gcccatggcc
    42781 gaaaggagag atgattattg cgaaagccct gggaactgac ccctgggtta tctggccatc
    42841 acgctaccat gatccgcaga cccatgagtt tatcgacaga acgcagttga tgcgtagcta
    42901 cactaaaccg aaaaaatgag tggtctggcg gtagccccgc gaacggggct gccagctctc
    42961 agacgattac tcgcctttca cacgctcaat atttgcacct aaagcgcgca gtttgtcttc
    43021 aatgcgttcg tagccacgat cgatgtgata aatacgatca accaccgtcg tcccttccgc
    43081 aatacagcca gccagcacca ggcttgctga tgcacgcaga tcggttgcca taacctgtgc
    43141 accagaaagt ttttcaacac catgacaaat aacggtattg ctttcgattt cggcgtgcgc
    43201 gcccatacgg ctcagctctg gcacatgcat aaagcggttt tcaaagaccg tttcggtgat
    43261 gaacccggtc ccttcagcca ccaggttcaa cagcgtgaac tgggcctgca tatcggtcgg
    43321 gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc ggacgtttgc catgcatatc
    43381 caggctaatc cagtcttcgc cgacttcgat gtccgctcca gcatcacgca gtttcgccag
    43441 cacggcgtcc agagtatctg gctgcgcgtt acggcagata attttgccac gagagatcgc
    43501 cgccgccacc aggaaagtac cggtttcgat acgatccggc agcacgcgat agacaccgcc
    43561 gcctaaacgt tccacacctt cgatgacgat acgatcggtg ccctgaccgc taattttcgc
    43621 acccagcgta atcaggaagt tcgcggtatc gacgatttcc ggttcacgcg ctgcgttttc
    43681 aataatcgtg gtgccttccg ccagggttgc agcacacatg atggtcaccg ttgcgccaac
    43741 gctgacttta tccatcacga tatgtgcgcc tttcagacga ccatcgacgg aagctttaac
    43801 gtaaccttct tccagtttga tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc
    43861 aaccggacga gcaccgatcg tacaaccgcc aggtagtgaa acttgcccct gaccaaagcg
    43921 cgctaccagc ggccccagcg cccagataga agcacgcatg gttttaacca gatcgtaagg
    43981 tgcgcagaat acattaacgt cgcgggcatc aatatgcaca gaaccattac gttctacttt
    44041 cgcacccagc tggcttagca gtttcattga tgtatcgacg tctttcagtt tcgggacgtt
    44101 ctggatctct accggttctt ccgccagtag tgcggcaaaa agaataggca gagcagcatt
    44161 tttagcgccg gaaattgtga cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt
    44221 atccatttag tttgttctca gttaacaatt catatccgct accggcgaat cgcccatagc
    44281 tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca ggggtatacg ctttgatcga
    44341 cacagcatga atgcggttat ccgcaatata ttccatcagc gggccataga ccgtctgctg
    44401 ttttttaacc cgactcatac cgtcaaacaa ctcacccacg gcaataacct gaaagtgact
    44461 gccatcgccg gaaacgtgga cttcctggag ggagagagcg ttcatcaaca cgctctgaat
    44521 ttcattattt tccatgggat cttcaatcat cagttaataa accagcgaaa catcttagag
    44581 caaagttgcg ctggcataaa taagcaaaaa gcctcgctga taaatcagac aaggctcgac
    44641 ttgcaggcag gtttgccgga caggcggtta acgccatatc cggcctgaaa aaatttaacg
    44701 aggcagaaca tcagcaggca aattatacaa tttcgcgagg gtatacactt tgtcgtttac
    44761 cccctgaagc gtcacgttgt tgccctgctt tttcgccaga tcgataagat ggagcagcag
    44821 tgccagtccc cccgtatcca cgcgggacac acggctaaga tcgatgcagg taatcccctt
    44881 caccgcttcc tcacgcattt cccaaagcgg tagcagaacg tcctgatcca gctctccgga
    44941 taacgccagc gtgtcacccg tctgcatcca gctcagtgac tcgctcatta ttttttctct
    45001 tccagagtga ttttctgttg agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct
    45061 ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg tgatcatact gacgccttca
    45121 gcaatcatgt cgtaagcttg ccagtttccc gtctgggagt ttttacgcca ctggaagtcc
    45181 agacgcaccg gcggacggcc attcgggtca ataatggtaa cgcgaatagg cacaatggtt
    45241 ttatcgccca gcggctgttc tggcgcaatc tgataggttt gaccgtgata catcgccagc
    45301 gcctgaccgt aagcctgctt caggtactca cggaaagcgg caaagtaggc ttcacgttga
    45361 gcaggggtcg cactcttgta atactggccc agcaccagcg caccggcgta tttcacctgt
    45421 acgtatggca gcagttcctg atcaacaatg gtacgcagat aatccgggtt ggcccgaatt
    45481 tgcggttgct cattcttcag gcgatcgaac gttttctgcg ccgcctcgtc catcagctta
    45541 taaggattgg tctggtctgc cgcggttgcc gcactcagag gtgcaatcac cagcaaagcg
    45601 accatcatta aacgtttaaa catgcgtcgg ttctcctgaa attatttcgt tgtacccaca
    45661 ggttcagtgg tttcattatt acctggcgca gcagctggcg catcgccact attcttattg
    45721 tcatcgcctt tactaccgta aaggaactga ccaatgagat cttccagcac catcgcagac
    45781 ttggtgtcct gaattgtatc gccatccttc aggatagcag tccccagttc cgggtcttca
    45841 aaaccgacgt ttaatgccag atattgttct cccagcaggc cggaagtacg aatgctcagc
    45901 gaactggtat caggaatgtg gttataacgt tgttcaattt ccagcgttac gcgcggcaga
    45961 taggttttcg ggtccagcgt aatatccgcc acccggccca caacaacacc accaatgctg
    46021 accggagagc gcgctttcag gccgccaatg ttatcgaacg tcgcataaag cgtgtaggtc
    46081 gattctgtac gtatggacgt cacgttcgcc gccttcaggc aaacaaacag cgccgccagc
    46141 agtgctgcta ataaaaagat acccacccaa atttcatttt ttttcgtttg catgaactca
    46201 attcccaaac atcaatgcgg tcagcacaaa atccagcccc agaacagcca gagacgagtg
    46261 gacaacggtg cgagtggttg cccggctaat cccggcagac gtcgggatgg cgtcgtagcc
    46321 gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc acgctcttaa tcagacagtt
    46381 gaccagatcc atacgccagt cgacggcatt ttgcattgcc gaccagaaga acccgctatc
    46441 aatgcctttc caactgacgc cgaccagcga tccgccccag atccccacgg cgacgaagat
    46501 aaccgtcaac agtggtaatg aaataacccc agcccagaaa cggggagaaa taacccgacg
    46561 cagcggatcc accgccatca tctccatact ggagagttgc tctgtagcgc gcatcaggcc
    46621 gatttctgcg gttaacgccg aaccagcacg cccggcaaac aacagcgcgg caaccaccgg
    46681 ccccagttca cgcagtagcg ataacgccac cagcataccc agactggttt ccgcgctata
    46741 agtggtcaga accagataac cttgcagccc caacaccatt ccgatgaaca cgccagaaac
    46801 tacaataatc agcatcgaca ggacgccgac attatagagc tggcgcacca gcagcggcgc
    46861 atgtttgcga aattccggtt tgccgaccag cgcattgaat aacattaacc cggcccgccc
    46921 gaacgttctc agggttttaa tccctttatg tccgagcgac gccagcgcat ttaacagcat
    46981 gagtggctta actccctggt aaaagatcag cgtgataatc gccggcagga tagcggaacg
    47041 gaacaggccc gtcagctatc ccgtccagaa actgacgtac gcgcggatca ggattcgtct
    47101 gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc cgccaggatc caggcgtgat
    47161 ccgcaatact taacacttcc ggcacatcgt gagaaaccac cacacaagtc acgcccagcg
    47221 cgctgttcag ctcagaaatc agcttcacca gtacgcccat ggtgatggga tcttgcccaa
    47281 caaaaggttc atcaaacatg atgagatccg gctccagcgc aatcgcacgc gccagcgctg
    47341 cacgccgcgc catcccaccg gaaagttcag aaggcattag tttagccgct ccacgcagcc
    47401 ccacggcctc cagcttcatc atcaccgtac tatgcaacaa tggcgcggga agttgggtat
    47461 gttcgcgcag tggataggcg acgttgtcaa atacgttcat atcagtgaac aacgccccgg
    47521 actgaaataa catgctcatc cgtttacgca ctgtatacag gcgcgaacga gacatcgccg
    47581 gaatattctc accatcgaaa aggatctcac catgatctgg tgcgatttgc ccgccaatca
    47641 gacggagtag cgtcgtttta ccgatgcccg atggccccat gatcgccgtg atcttccctc
    47701 gcggcacggt cagggaaata ttatcgaaga tgcagcgatt gccacgcgta aaactgacat
    47761 cgcgcatatc gactaaattc gccacagact gctccataat tcacccttcg tcttgcgttg
    47821 attttctaag catggcgctc aatttaacct tgaacccaac atatttacag aatattaccc
    47881 gccgtggtta gcgaaagctg gcatttgttt tactttttag ccgcataaag tcaaaattaa
    47941 gaatccgtta cggctttctg aaaatcttca gcggaccggc gagtatacct gaagaaagga
    48001 cgttagatgc ttttagctac ggcactgtta attgttggtt tacttttggt cgtttacagt
    48061 gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa cctttggcat cccgccgctg
    48121 atcatcggca tgacggtggt cagtattggt acatcgttac cagaaatcat cgtctcgctt
    48181 gccgcgtctc tgcacgaaca acgcgattta gccgttggta cagccctcgg ctcaaacatt
    48241 atcaatatat tgctgatcct cggtctggct gcgctggttc gtccttttac cgtccattcc
    48301 gatgttctgc gccgtgaatt acccttaatg ttgttggtca gcgtggtggc cggttccgta
    48361 ctctatgacg gacaacttag tcgaagcgat ggtatctttc tcctctttct ggctgtgcta
    48421 tggctgctgt tcattgttaa acttgcacgt caggggactg acagcctgac cagagagcag
    48481 cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct cggcattgcg
    48541 cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt gctggcgaat
    48601 tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg aaccagtctg
    48661 ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat tgctgtcgga
    48721 aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata
    48781 acgccaggag agattgatcc actggcgtac agtcgtgact acagcgtgat gttgctggtg
    48841 agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg
    48901 gtattattaa ctggcggatt tatcatatgg ctggcgatgt tgtactggtt atcgccaata
    48961 ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg gttttgactt
    49021 tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca
    49081 atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt
    49141 tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg caacgtttgc
    49201 cagcaccggt acaccttcat ttttcgtcca tcctagtgaa gccgcgcatg gtgatttagg
    49261 catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat ccagcgaaat
    49321 cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca tcaccggtcg
    49381 cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga
    49441 agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg ttatgggcga
    49501 tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt ttgcgctctc
    49561 acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac
    49621 gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcat tgctggaagt
    49681 tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga ttgaaggcat
    49741 ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc gtcagttaag
    49801 tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc tggccgttga
    49861 ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg atggcgacca
    49921 tttactcggt gtgttacata tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa
    49981 ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc
    50041 gccgacgtta tggcaaaagc agagaacatt cgtctgctga tcctcgacgt tgatggcgta
    50101 ctgtcagatg gcctgattta tatgggcaat aacggcgaag agctgaaagc gttcaatgtt
    50161 cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc tatcattacc
    50221 gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac tcacttgtat
    50281 caggggcagt caaacaaact gatcgccttt agcgatttgc tggaaaaatt ggcgattgcc
    50341 ccggaaaatg tggcttatgt tggcgatgat ctcatcgact gcccggtaat ggaaaaagtg
    50401 ggtttaagcg tcgccgtggc cgatgcccat ccactgttga tcccgcgcgc cgattacgtg
    50461 acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt actcctggcg
    50521 cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga cgttgggtta
    50581 tcattgtgct atcactggcg gttctggtga tgatcggcat taatatggcc gaaaaagacg
    50641 ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag catacggaca
    50701 cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa cacgttgaat
    50761 attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg tttgataagg
    50821 ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat gaccggatgc
    50881 tctatttata tggacacgtt gaagtcaacg cacttgtgcc agactctcaa cttcgcagaa
    50941 tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct gaagacctcg
    51001 tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc aacttacgca
    51061 gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa
    51121 ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc ggcatttgcc
    51181 gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc tcttgatatg
    51241 caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac catcaaaatt
    51301 aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga agtgattgac
    51361 ggatacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc cgttgaaggt
    51421 cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac gggtaatgct
    51481 tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct ggtgaaagag
    51541 cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct ggtgccgtcg
    51601 cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg taattaattc
    51661 gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta
    51721 gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg gccaaacggt
    51781 gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga tgcgggcaac
    51841 atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc gcgccgcggt
    51901 atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta cgataacctg
    51961 atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga ccgcgcgaac
    52021 gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca gtcactctcc
    52081 gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt
    52141 ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgatat taaacgcatc
    52201 attgaacacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa cgtgcgtgaa
    52261 acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat cgcccacggc
    52321 acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg ggaagacttc
    52381 agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt ctgaacatga
    52441 agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag ctccaacagg
    52501 caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag caggcgctgg
    52561 agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc cgcgaaacgc
    52621 aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg ccggaagagc
    52681 tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc ggcaccagcg
    52741 gtgactacat tgacgacgag ctgccggtct accagggcga aacgacgcag accttgcagg
    52801 attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc gctattgcta
    52861 cctctatcgt cgatgccgtt gatgaaaccg gttatctgac tgtcccgctg gaagatattc
    52921 tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc cttaagcgga
    52981 tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc ctgctaatcc
    53041 aactctccca attcgataag accacgccgt ggctggaaga ggccagattg atcattagcg
    53101 atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc acgcgtctga
    53161 aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg cgccccgggc
    53221 agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg cgtaagcata
    53281 acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa atcaaccagc
    53341 actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt atccgcagca
    53401 atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat acgctactgc
    53461 gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt gaagaatata
    53521 tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa tcgacgatat
    53581 ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa ctgaagtatt
    53641 tcttttccag tcacgtcaat accgagggcg gcggcgaagc ttcctccacg gcgattcgtg
    53701 cactggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc gacagcaagt
    53761 taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt gcgaagtacc
    53821 gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat
    53881 aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac cgaggcactg
    53941 cgcgaatttg ttacagctaa atttgccaaa cttgagcaat attttgaccg aatcaaccag
    54001 gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac actgcatgta
    54061 aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc
    54121 ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa acaacactaa
    54181 ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta
    54241 cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg tgaaattatg
    54301 acaaataatg atacaactct acagcttagc agtgttctta acagggaatg tacgcgaagc
    54361 cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa
    54421 caacttagcc taccgcctca ggtggttttt gaagctatcc tgacgcggga aaaaatgggc
    54481 agtaccggta tcggcaatgg tattgccatt ccgcatggca aacttgaaga agatactctg
    54541 cgtgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc catcgacaac
    54601 caaccggtgg acttactttt tgccctgctg gtgccggcag accaaactaa aacgcacctg
    54661 catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc
    54721 gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg tactccggat
    54781 gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca gtacatggta
    54841 ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa
    54901 gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga tctggctcga
    54961 actctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa tatgccggag
    55021 tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc accgcaacta
    55081 ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac gcgccgactg
    55141 catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga aagcgatttg
    55201 ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat gtccgttcac
    55261 gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga actgaccatg
    55321 gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta cgtctttgac
    55381 gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac aggtcttgat
    55441 aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat ctaccagacg
    55501 cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag ctacctgacg
    55561 gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga gcaactggca
    55621 gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt
    55681 aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg catgcccggc
    55741 ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg
    55801 acgaaggcac cgaggctgaa gccaacagcg ttattgcgct gctgatgttg gattctgcca
    55861 aaggacggca gattgaagtt gaagcgaccg gtccacagga agaggaagca ctggccgccg
    55921 ttatcgccct ctttaattct ggttttgatg aagattaatc ttcatcactt tgacatacaa
    55981 ctcccttcaa gctcccccga caataagaaa atcacgtact gaaatcgttc tcaatcaacg
    56041 tcatttgtac attttgtgcg cttttcactt tcagaagaac cttaagaaaa ccttaagagg
    56101 cattgtttag gttttgttta agttaatcga ccatactgga gatcgtcaga aaatatttcc
    56161 aggagatggc atgattcgct tatctgaaca aagtcccctg ggcaccggaa gacatcggaa
    56221 atgttatgcg catccggaag atgcccaacg ctgtatcaag attgtctacc atcgtggcga
    56281 tggtggcgat aaagagatcc gccgtgagtt aaagtactac gcgcatcttg gtcgccggtt
    56341 aaaagactgg agtggaatac cgcgctatca cggtaccgtt gagacggatt gcggaaccgg
    56401 ttatgtctac gatgtgattg ccgattttga cggcaagcct tccattacgc tgaccgaatt
    56461 tgctgaacaa tgccgttacg aagaagacat tgcacaactg cgccagctac tgaaacagct
    56521 gaagcgttat ttgcaggata accgtatcgt gacgatgtcg ctgaagccgc agaatatcct
    56581 ctgtcaccgc atcagtgagt cagaggttat cccggtcgtc tgcgacaaca ttggtgaaag
    56641 cacattgatt ccactggcga cctggtcgaa gtggtgttgt ctgcgtaagc aagagcggtt
    56701 atggaaacga tttatcgccc aaccggcgct ggctattgcc ctgcagaaag atctgcaacc
    56761 tcgtgagagc aaaacgctgg ccctcacttc gcgcgaagct taatccagct ggtgttgttg
    56821 cataaacggc tcaccgccta actgatacat ctgccgtaaa atccacgcct gacggctacg
    56881 cacgtagccg tcaggcgtgg agactttgaa acgaagtgga ttaggtaata cagctgccag
    56941 taatgcagct tccgaccggg taagtttgct cgcgggtttg tggaaataac gttgtgccgc
    57001 agcttcgacg ccaaacacac cgtcgccaaa ttcggcgata ttcaggtaaa ccgtcaggat
    57061 acgctttttg ctccagactg tttctatccc cagcgttaat ccagcttcca gcccttttcg
    57121 tacccagcta cgcccatccc ataaaaagag atttttggcc gtctgctgag aaatcgttga
    57181 agcaccgcga atacggtttt cattgcgctc gttgtgcgcc agggctttct caatggaagc
    57241 gacatcaaag ccccagtgct caggaaattt ctgatcttct gcggcaatca ccgccagtcc
    57301 catccacggc gagatttgat ccatactgac ccagtcagaa tgtgccacgt aacgaaaatt
    57361 gccatgcagc caggcgctga cctgtcgctc gaccattacc gctgagaagg gaacaggcgc
    57421 aacgctaaac aacgcgatgc ccccgcccca gaaaacggcg aggacaacca ttaaccgcaa
    57481 aagaaaacgg cgaacgaaac taaacaccgt taagcggctt ttactcattc agccagaacc
    57541 agcacgcggg aaaccagctt atcaatgccg ctcgccgctt ctgcaatgtt ctgcgccagc
    57601 atatatgctg gggtggtgac aatcttattg tcttcatcaa ccacgatatc atcgacagga
    57661 cacggcacat gctccgcgcc catctcttcc agtacttctg cggtatcgat atcagtaccg
    57721 atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca tcgccggggc aatacacata
    57781 aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca gcgcctttaa ttcacggtca
    57841 acggtgcatt cgctaccaag actggcaaaa ttgcttaaat tcttcgccgc gccaaccccc
    57901 cccggcacaa tcaacgcatc cagttcagcg gcatcggcct gggccagagg acggatttca
    57961 ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg tttccgtcat cgcttcgcca
    58021 gttaaatggt tgataacatc aacctgctgc ttatccggtg caaagcagac cgcctgcgca
    58081 ccgctgcgtg aaatagctaa caacgtcaac accgcttcat gaatttcaga accgtcatag
    58141 acgccgcatc cgctcagaat tacgccaatt ttcttcattg tgatcatcct ttcgcaattg
    58201 actgaaacac attaattttt ttaataaaaa tggtacgcat cacacattta actgattcat
    58261 gtaacaaatc atttaagttt tgctatctta actgcgtgcg gcctgaaaaa cagtgctgtg
    58321 cccttgtaac tcatcataat aatttacggc gcagccaaga tttccctggt gttggcgcag
    58381 tattcgcgca ccccggtcta gccggggtca ttttttagtg gcttttgcca cccacgcttt
    58441 cagcacttct acgtcgtgac gccactcttc tttcatctct tcaatccatt caccgacgtt
    58501 atcttcccag gcgggaaggt caggagactg aatttgctga cccagctgtt gcagatggcg
    58561 taaccccact gaccccgccg cacctttaat tttatgtcct tcctcaacaa tgcctttttt
    58621 atcctgcgcc gtcagattcg actccagcac gctgacatag cccggcatca tcttctcaaa
    58681 caccgctaac ccgtcggtga tcagcttcgg tcctacaagt tcgagatact gttccagcat
    58741 gggaatatcg agcaatgctt ctgatttact gttctcttct gtcgtcaccg tactcttctc
    58801 atcatcctgg gtatcccaga atttcttgat catcgcggtt agcgccggaa ccgaaagcgg
    58861 cttactcagc acatcatcca ttccagcatt gaggtactct tgtttgtctt tcagcacgtt
    58921 agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga taacgcttcg tcagttcacg
    58981 agagatatcc agcccggtca tatctggcaa ctgaatatcc agcaacacca ggtcgtattc
    59041 gcccggttta aacatctcca gcgccgcctt gccggtcatg gcgacatcaa cgctgttacc
    59101 taatttttcc agcacagaac gcgcgacaat cacgttcagt tcaatgtctt ccaccagcag
    59161 cacattcagc gccggtaaag gcatatcgtc ttcatcaaac gcatcatcga cctcttctgc
    59221 caccgacggt gcgtggatcg tcaacgtaaa ggttgaacct ttgccctgtt cgctggtaac
    59281 cgtaatatcg ccgcccatat ttttcgccag acgacgagaa acggccagac caataccggt
    59341 gccggtggca ggtttaccgc catgactgtc tttcacctgg taatacatgg cgaagatttt
    59401 atccagctca tcctgcggaa tgccgatacc agaatcttcc acttcaaaat gcagcatatc
    59461 gccttcatcg tagcgcacgc gcacggtaac ctggccttgc tgggtgaatt tgacggcgtt
    59521 actgatgagg ttccacagga tctgccgtaa acgcgtcccg tcggtaatga cctgatgcgg
    59581 taatggcagc gtaggctcca ggttaaagcg caatcctttt tgttgcgcct gcaatgcgga
    59641 gagattttcc agatcggcaa ggaagctggt gaaatcaacc ggctgattat caagctggac
    59701 cttgcgccgt tccatcttat ccatgtcgat aatatcgtta aagatattcc ccagcgtgac
    59761 ggccgaaaca tggatagttt tgagatattt ttcctgctcg gcggtgagtt cggtatccag
    59821 cagaatgcgg ctcaggccga cgataccgtt cagcggtgta cgcaattcgt gactgatggt
    59881 ggaaataaac gtcgttttgt cgcggctggc gcgttcaagc gcatcctgat accgcttacg
    59941 ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt ttacccacgc ggtcgtagta
    60001 cggcacttta cggatttcaa agcaggcttt gcgcccgtcc gggtaatcca gccactgttc
    60061 ataggtcagt gacacattat gacggaacac tttttcatcg gtttcaatga cttttgcggc
    60121 ggcttccggt gagtaaacat cagcaggttt cagatgaacc agttgttttt cgctttttcc
    60181 ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct ttatcttcgt tacgataaaa
    60241 aaccaagtcg ggtgaagcat caaggaagga acgtaagaat gaggattgct gctcgagctg
    60301 aatttgtgtc tcttcgcgct ctttgatttc aattttcagt tggccgaagg tttcctgcag
    60361 ttctgcttcc gctttttcac gaacggcaat ttcctgattt agctgggcaa tattatcttt
    60421 taactgaacg ttgaggctca aatcgcgctc gcgcatctcc tccagttttt gcaccagccg
    60481 tgacagacgt tgtcgtgact cctccagttg ctcgacgacc accgatagaa agtagaccgc
    60541 ccacggcgta atcagcaaac caaagaagat agaacgaata acatcaatgc tttcgacctg
    60601 accatgcagc accatggtta ccgccatttg taccacaatg gcaagaacga cgagggccag
    60661 cgccagcaac attgagaagc gcaccagacc taacttcatc atcaggtcaa cataatactg
    60721 cgccagcaga cgaatttgct tcattaggga attccttcac gacaacctgc cccaataata
    60781 cccaattctg acagttacgt tataaattgt gtgagaaatg cggaattccc ctcaacgacc
    60841 tactccgttg gaggtagcca cggacgtccc agcgccgatc cctgtacgcc atgttcattc
    60901 agatagcgat ccagctcgac cattcccgtc catcgatttt cgcaccacag cggagcaagc
    60961 agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga tcacttccgg tggcgtatgg
    61021 cgaatcattt ctccggcagt gagcgtataa tcctccagtt caataccgtt caaacgcccc
    61081 gcttcccagg ctttcgccat aatgctgcct ttcacaatat gcagcggatg cagctttatg
    61141 ccgtccacgc tggtttcaac cactcgctcc agcgtttgca aacattcagc ctgcccttcg
    61201 ccaggcaggc cgacaattaa gtgggaacac accttcagcc cgcgctgacg tgccagctgg
    61261 gttgtacgct gataacaggc aaaatcatgg ccgcggttga tgcgatgcag tgttttgtcg
    61321 tgggcggttt gtagccccag ctccagccac acttcgtaac cctggtcctt atattcgcaa
    61381 agcagatcca gcaccgcatc cggcacgcag tccgggcggg taccaacaca caaaccgaca
    61441 atattggcct ggctcaccgc ctgctgatac atagaacgca gcacctgaac ttccgcaaag
    61501 gtgctggtat acgcctgaaa gtaggccaga tagcgtttag cgcggttaac taaattcgcc
    61561 tggtgcgcca gttgctcggc aatggaacga tgctgctgcg cttcatcggc aaacgaggca
    61621 acattacaga atgtgcagcc accacgcccg atggtaccgt cacggttagg gcagctaaaa
    61681 ccgccgtgca gcgtcagctt atgcaccttt tgcccataac gacgggtgag atcaccacca
    61741 aacatattga ctaatttctg taactgcata atctgataga ccgcgccttg aaaagaggcc
    61801 aaagcctgcc atttttagct caattcggcg atgacctaga tcaatcgtcc cgccctgctt
    61861 ttatcaactg cataatcaat caaaattacc gaaatttcat gcataatcac ataaatcact
    61921 tttgcttatc ttgtgtcaga tttttttatc tccttatgga ttttaggcaa aaacagtagc
    61981 atgaaacgtc attaccaatt aaggcagtat aaaatgctgg ttttctcgtc agttcaaggc
    62041 aggataaggg ttaacacgcc tttatgacag tcaggaattg actgtttctc taacgactct
    62101 ccttttagcc ttaaagataa aatctatttt aatttcagcc atttaataaa gaattttgcg
    62161 ctaaagcaca tttctgtacc aataagcttg ccatttgacc tgtatcagct ttcccgataa
    62221 gttggaaatc cgctggaagc tttctggatg agcagcctgc tcatcatatt tatgcagtaa
    62281 ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg acaacaaggg ggcgaatgcg
    62341 aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt tctgtgcccg tgcgcagcgg
    62401 ttcggaagtg gggttcccgc agagcctggg ggaggttcac gatatgttgt acgataaatc
    62461 ccttgagagg gataactgtg gtttcggcct gatcgcccac atagaaggcg aacctagcca
    62521 caaggtagtg cgtactgcaa tacacgcact ggcccgcatg cagcaccgtg gcgcgattct
    62581 cgccgatggt aaaaccggcg acggttgcgg cttgctgtta caaaaaccgg atcgcttttt
    62641 tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa aactacgctg tcgggatgct
    62701 cttcctgaat aaagatcctg aactcgccgc tgccgcacgc cgcatcgttg aagaagaact
    62761 gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc cccactaacg aaggcgtgct
    62821 gggtgaaatc gccctctcct ctctgccacg cattgagcaa atttttgtga acgccccggc
    62881 aggctggcgt ccacgcgata tggagcgccg tctgtttatc gcccgccgcc gcattgaaaa
    62941 gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg tcgaatctgg tgaacatcta
    63001 taaaggtctg tgtatgccgg cggatctgcc gcgcttttat ctggatcttg cggacctgcg
    63061 tctggaatcg gccatttgcc tgttccacca gcgcttctcc actaacaccg taccgcgctg
    63121 gccgctggcg caaccgttcc gctatctggc gcataacggt gaaatcaaca ccatcaccgg
    63181 taaccgccaa tgggcgcgtg cgcgtaccta taaattccag acgccgctga tccctgacct
    63241 gcacgacgcc gcgccattcg tcaacgaaac cggttctgac tccagttcca tggataacat
    63301 gctggaactg ctgctggcag gcgggatgga tatcatccgc gccatgcgcc tgttagtgcc
    63361 acccgcctgg cagaacaacc cggatatgga cccggaactg cgtgccttct ttgactttaa
    63421 ctccatgcat atggagccgt gggatggtcc ggcgggcatc gtgatgtccg atggtcgctt
    63481 cgctgcctgt aacctcgacc gtaacggtct gcgtccggca cgctacgtca ttaccaaaga
    63541 caagctcatc acctgcgcct ctgaagtcgg tatctgggat taccagcctg acgaagtggt
    63601 cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc gacactcgca gtgggcgtat
    63661 tctgcactcg gcagaaaccg atgacgatct gaaaagccgc cacccttata aagagtggat
    63721 ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg cccgatgaag aagtgggtag
    63781 ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa cagtttaact acagcgcgga
    63841 agagctggac tccgtaattc gcgtactggg cgaaaacggt caggaagcgg tcggttcgat
    63901 gggcgatgat accccattcg ccgtgctctc cagtcagccg cgcattattt acgactactt
    63961 ccgccagcag tttgcccagg tgactaaccc gccaatcgac ccgctgcgtg aagcgcatgt
    64021 tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc ttttgcgaag cagagggcca
    64081 ggcgcaccgt ttaagcttta aatcgccgat tctgctctac tccgatttca aacagctcac
    64141 gacgatgaaa gaggagcact accgcgcaga tacgctggat atcacctttg acgtcactaa
    64201 aaccacgctc gaagcgacag tcaaagagct atgcgacaaa gccgaaaaaa tggtacgtag
    64261 cggcaccgtg ttgctggtgc tctccgaccg gaatatcgct aaagatcgcc tgccggttcc
    64321 ggctccgatg gcggttggcg cgatccagac ccgtctggtc gatcaaagcc tgcgttgcga
    64381 tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg caccacttcg ccgtgttgct
    64441 gggtttcggc gcgacggcta tttatccgta ccttgcctat gaaacgctgg gccgcctggt
    64501 agacacccac gcgattgcca aagattatcg taccgtgatg ctcaactacc gtaacggcat
    64561 caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc tccaccatcg cctcttaccg
    64621 ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat gtagtgggcc tgtgcttcca
    64681 gggcgcggtc agccgcattg gtggggcaag ctttgaagac ttccagcagg atctgctgaa
    64741 tctgtcgaaa cgtgcctggc tggcgcgcaa gccgattagc cagggcggcc tgctgaaata
    64801 cgtccacggc ggcgaatacc acgcctacaa cccggacgtg gtgcgcacgc tgcaacaggc
    64861 ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg aagctggtta atgagcgtcc
    64921 gacaaccacg ctgcgcgatc tgctggcaat tacgccgggt gaaaacgcgg tcaacattgc
    64981 tgatgttgaa ccggcaagcg aactgtttaa acgctttgat accgccgcga tgtctatcgg
    65041 cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg atgaatagca tcggcggtaa
    65101 ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat ggcactaaca aagtgtcgcg
    65161 catcaagcag gtagcttctg gtcgcttcgg cgttactccg gcgtatctgg tcaatgccga
    65221 cgtcattcag attaaagtcg cccagggcgc gaagccgggc gaaggcggtc agttgccggg
    65281 cgacaaagtc actccttaca tcgccaaact gcgctattcg gtgcccggag tgacgctgat
    65341 ctccccgccg ccgcaccacg atatctactc tatcgaagac ttagcgcagc ttattttcga
    65401 cctcaagcag gttaacccga aagcgatgat ctccgttaag ctggtgtccg aaccaggcgt
    65461 tggcaccatc gcgactggcg tggcgaaagc ttatgcggac ttgatcacca tcgcgggcta
    65521 tgacggcggc accggcgcaa gcccgctgtc gtcagtgaaa tatgcaggct gcccgtggga
    65581 actcggtctg gtggaaaccc aacaggcgct ggttgccaac ggcttgcgtc ataaaattcg
    65641 tttgcaggtt gatggcggcc tgaaaacggg tgtcgatatc atcaaagcgg cgattctcgg
    65701 cgcagaaagc ttcggcttcg gcactggccc aatggtggcg ctcggctgta aatatctgcg
    65761 tatttgccac ctgaacaact gcgccacggg tgtagcaact caggatgaca aactgcgtaa
    65821 gaatcactat cacggcctgc cgttcaaggt gactaattac tttgagttta tcgcccgtga
    65881 aacccgcgag ctgatggcac agctgggagt aacgcgtctg gtggatctga ttggtcgcac
    65941 cgacctgctg aaagagctgg acggtttcac cgccaaacag cagaaactgg cgctgtcgaa
    66001 gctgctggag actgccgagc cacatgcagg taagtcactc tactgcactg aaaacaaccc
    66061 gccgtttgat aacggcctac tgaacgcgca gttgctgcaa caggcgaaac cgtttgtcga
    66121 tgaacgccag agcaaaacct tctggttcga tatccgcaac accgaccgtt ctgtcggcgc
    66181 gtcgctttca ggctatatcg cccagacgca cggcgatcag gggctggcag ccgatcctat
    66241 caaagcgtac ttcaacggca ccgcaggcca gagtttcggt gtgtggaacg cgggcggcgt
    66301 ggaactatac ctgaccgggg atgccaacga ctatgtcggt aaaggcatgg cgggcggctt
    66361 aatcgccatt catcctccgg ttggttccgc cttccgcagc catgaagcaa gcattatcgg
    66421 caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat gccgcaggcc gcgcgggtga
    66481 acgtttcggc gtgcggaact ccggtgctat caccgtggta gaaggcattg gcgacaacgg
    66541 ctgtgaatat atgacgggcg gtattgtctg tattctgggt aaaaccggcg ttaacttcgg
    66601 cgcgggcatg accggcggct tcgcttacgt tctcgatgaa agcggtgatt tccgcaaacg
    66661 cgttaacccg gaactggtcg aagtcttaag cgtcgacgat ctggcgatcc atgaagagca
    66721 tctgcgcggt cttatcaccg agcatgttca gcataccggt tctcagcgcg gtgaagagat
    66781 tctggcgaac tggtcaacct tcgccacgaa attcgcgctg gttaaaccga agtccagtga
    66841 tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca gagttgcgcg tgcaggcgca
    66901 gtaaggggta gcaacaatga gtcagaatgt ttatcaattt atcgacctgc agcgcgttga
    66961 tccgccaaag aaaccgctga agatccgtaa aattgagttt gttgaaattt acgagccgtt
    67021 ttccgaaggc caggccaaag cgcaggctga ccgctgtctg tcgtgcggca acccatactg
    67081 cgagtggaaa tgcccggtac acaactacat cccaaactgg ctgaagctcg ccaacgaggg
    67141 gcgtattttt gaagcggcag aactgtcgca tcagaccaac acactgccgg aagtatgcgg
    67201 acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc accctgaacg atgagttcgg
    67261 tgcggtgacc atcggcaaca ttgagcgtta tatcaatgat aaagcgttcg aaatgggctg
    67321 gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa gtggcgatta tcggtgcagg
    67381 cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac ggcgtaaaag ccgttgtctt
    67441 cgaccgtcat ccagaaattg gcgggctgct gaccttcggt attccggcct tcaagctgga
    67501 aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc atgggtattg aattcaaact
    67561 caataccgaa gtgggccgcg acgtacagct ggacgatctg ctgagtgatt acgatgccgt
    67621 gttccttggc gttgggactt atcagtcaat gcgcggcggg ctggaaaacg aagacgccga
    67681 tggcgtgtac gcagcgctgc cgttccttat cgccaacacc aaacagttaa tgggctttgg
    67741 tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa cgcgtggtag tacttggcgg
    67801 tggcgacact gcgatggact gcgtgcgcac gtctgtacgc cagggggcaa agcacgttac
    67861 ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc cgccgcgaag tgaaaaacgc
    67921 gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca ctggggattg aagtgaacgg
    67981 taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa atgggcgaac cggatgccaa
    68041 aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat atcgttccgg cagatgcggt
    68101 gatcatggcg tttggtttcc gtccacacaa catggaatgg ctggcaaaac acagcgtcga
    68161 gctggattca caaggccgca tcatcgctcc ggaaggcaac gacaacgctt tccagaccag
    68221 caacccgaaa atctttgctg gcggcgatat cgtccgtggt tccgatctgg tcgttaccgc
    68281 catcgccgaa ggtcgcaaag cggcagacgg cattatgaac tggctggaag tttaagctct
    68341 gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa ttctcctttc tttctggaaa
    68401 tcgcctcgca taatctgaag aaaaccaatg taatacgctc atctggattg ttaccggtag
    68461 atgcccggcg tatagctgat tccattattc aacgcagcgc catataaagg gacagataat
    68521 ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa atccacgacg gcattattca
    68581 tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt aacgcgctga tgaccgcgac
    68641 gtactgggaa attggccgac ggattgtcga atttgaacaa ggtggtgagg ccagagcagc
    68701 ttatggtgca cagttgattg atcggctatc gaaggattta agtcgacggt ataaacgtgg
    68761 ggtctctgca aaaaacttac gccaaatgag gcttttttac ctcttttttc aacatgttga
    68821 aattcgccag acagtgtctg gcgaattaac accattggga attccgcaga caccatctgc
    68881 agaattccca tcggcaaa
//
LOCUS       DAJTXY010000006        68182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTXY010000006 DAJTXY010000000
VERSION     DAJTXY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.006"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..245
                     /locus_tag="OO566_000490"
     CDS             <1..245
                     /locus_tag="OO566_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6603900.1"
                     /translation="TIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIE
                     QRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(292..1050)
                     /gene="deoR"
                     /locus_tag="OO566_000491"
     CDS             complement(292..1050)
                     /gene="deoR"
                     /locus_tag="OO566_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6603901.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWLIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1108..1704)
                     /gene="ybjG"
                     /locus_tag="OO566_000492"
     CDS             complement(1108..1704)
                     /gene="ybjG"
                     /locus_tag="OO566_000492"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6603902.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1989..3215
                     /gene="mdfA"
                     /locus_tag="OO566_000493"
     CDS             1989..3215
                     /gene="mdfA"
                     /locus_tag="OO566_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR6603903.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3256..3541)
                     /locus_tag="OO566_000494"
                     /pseudo
     CDS             complement(3256..3541)
                     /locus_tag="OO566_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OO566_000495"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OO566_000495"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6603904.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="OO566_000496"
     CDS             complement(4442..5650)
                     /locus_tag="OO566_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6603905.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="OO566_000497"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="OO566_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6603906.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="OO566_000498"
     CDS             complement(6445..8130)
                     /locus_tag="OO566_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6603907.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="OO566_000499"
     CDS             8400..8777
                     /locus_tag="OO566_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6603908.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OO566_000500"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OO566_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6603909.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="OO566_000501"
     CDS             9224..9511
                     /locus_tag="OO566_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6603910.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9495..10218
                     /gene="nfsA"
                     /locus_tag="OO566_000502"
                     /pseudo
     CDS             9495..10218
                     /gene="nfsA"
                     /locus_tag="OO566_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="OO566_000503"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="OO566_000503"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6603911.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="OO566_000504"
     CDS             11269..11745
                     /locus_tag="OO566_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6603912.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="OO566_000505"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="OO566_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6603913.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14380
                     /gene="potG"
                     /locus_tag="OO566_000506"
     CDS             13304..14380
                     /gene="potG"
                     /locus_tag="OO566_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6603914.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="OO566_000507"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="OO566_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6603915.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="OO566_000508"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="OO566_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6603916.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="OO566_000509"
     CDS             16302..16790
                     /locus_tag="OO566_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6603917.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="OO566_000510"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="OO566_000510"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6603918.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OO566_000511"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OO566_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6603919.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OO566_000512"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OO566_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6603920.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OO566_000513"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OO566_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6603921.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OO566_000514"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OO566_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6603922.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OO566_000515"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OO566_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6603923.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="OO566_000516"
     CDS             complement(22242..22757)
                     /locus_tag="OO566_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6603924.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="OO566_000517"
     CDS             23406..25175
                     /locus_tag="OO566_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6603925.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="OO566_000518"
     CDS             25386..25709
                     /locus_tag="OO566_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6603926.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="OO566_000519"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="OO566_000519"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6603927.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="OO566_000520"
     CDS             complement(26533..27546)
                     /locus_tag="OO566_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6603928.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVLTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="OO566_000521"
     CDS             complement(27645..29075)
                     /locus_tag="OO566_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6603929.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANKHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OO566_000522"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OO566_000522"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6603930.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OO566_000523"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OO566_000523"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6603931.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OO566_000524"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OO566_000524"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6603932.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OO566_000525"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OO566_000525"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6603933.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OO566_000526"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OO566_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6603934.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="OO566_000527"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="OO566_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR6603935.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     VSGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37227..38885
                     /locus_tag="OO566_000528"
     CDS             37227..38885
                     /locus_tag="OO566_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6603936.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38882..39838)
                     /locus_tag="OO566_000529"
     CDS             complement(38882..39838)
                     /locus_tag="OO566_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6603937.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39989..41104
                     /gene="macA"
                     /locus_tag="OO566_000530"
     CDS             39989..41104
                     /gene="macA"
                     /locus_tag="OO566_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6603938.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41101..43047
                     /gene="macB"
                     /locus_tag="OO566_000531"
     CDS             41101..43047
                     /gene="macB"
                     /locus_tag="OO566_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6603939.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="OO566_000532"
     CDS             complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="OO566_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6603940.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43667..43987
                     /gene="clpS"
                     /locus_tag="OO566_000533"
     CDS             43667..43987
                     /gene="clpS"
                     /locus_tag="OO566_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6603941.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44018..46294
                     /gene="clpA"
                     /locus_tag="OO566_000534"
     CDS             44018..46294
                     /gene="clpA"
                     /locus_tag="OO566_000534"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6603942.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46697..46784)
                     /locus_tag="OO566_000535"
     tRNA            complement(46697..46784)
                     /locus_tag="OO566_000535"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46748..46750),aa:Ser,seq:gga)
     gene            complement(47038..47256)
                     /gene="infA"
                     /locus_tag="OO566_000536"
     CDS             complement(47038..47256)
                     /gene="infA"
                     /locus_tag="OO566_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6603943.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47541..48245)
                     /gene="aat"
                     /locus_tag="OO566_000537"
     CDS             complement(47541..48245)
                     /gene="aat"
                     /locus_tag="OO566_000537"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6603944.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="OO566_000538"
     CDS             complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="OO566_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6603945.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTQLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="OO566_000539"
     CDS             complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="OO566_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6603946.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="OO566_000540"
     CDS             complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="OO566_000540"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6603947.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53408..53902
                     /gene="lrp"
                     /locus_tag="OO566_000541"
     CDS             53408..53902
                     /gene="lrp"
                     /locus_tag="OO566_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6603948.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            54037..58143
                     /gene="ftsK"
                     /locus_tag="OO566_000542"
     CDS             54037..58143
                     /gene="ftsK"
                     /locus_tag="OO566_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6603949.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            58298..58909
                     /gene="lolA"
                     /locus_tag="OO566_000543"
     CDS             58298..58909
                     /gene="lolA"
                     /locus_tag="OO566_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6603950.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58920..60263
                     /gene="rarA"
                     /locus_tag="OO566_000544"
                     /gene_synonym="mgsA"
     CDS             58920..60263
                     /gene="rarA"
                     /locus_tag="OO566_000544"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6603951.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60354..61646
                     /gene="serS"
                     /locus_tag="OO566_000545"
     CDS             60354..61646
                     /gene="serS"
                     /locus_tag="OO566_000545"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6603952.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61886..64330
                     /gene="dmsA"
                     /locus_tag="OO566_000546"
                     /pseudo
     CDS             61886..64330
                     /gene="dmsA"
                     /locus_tag="OO566_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64341..64958
                     /gene="dmsB"
                     /locus_tag="OO566_000547"
     CDS             64341..64958
                     /gene="dmsB"
                     /locus_tag="OO566_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6603953.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     HYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            64960..65823
                     /gene="dmsC"
                     /locus_tag="OO566_000548"
     CDS             64960..65823
                     /gene="dmsC"
                     /locus_tag="OO566_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR6603954.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(65859..66485)
                     /locus_tag="OO566_000549"
     CDS             complement(65859..66485)
                     /locus_tag="OO566_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6603955.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            66800..67948
                     /locus_tag="OO566_000550"
     CDS             66800..67948
                     /locus_tag="OO566_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6603956.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT        16943 a        16855 c        17854 g        16530 t
ORIGIN      
        1 tgaccatacc gcgtgggcag ctgaaaaacc tgaaggcgag ctacacgtta acggaaccgc
       61 agcttaccgc accgctgaaa aaaggtcagg ttgtcgggac cattgatttc cagcttaacg
      121 gtaaatccat tgagcagcgt ccgctgatcg tgatggaaaa tgtggaagag ggcggattct
      181 ttggtaggat gtgggatttc gtgatgatga aattccatca gtggttcggc agctggttct
      241 cttaatcttc tgataaccgg atggcgcgaa acgtcatccg gttatacgtc attaatacat
      301 caacttaata cggtgcgtct gcgcgtactt cacatactca tcttccgggc aacaatcact
      361 caccacaata tcaaagcgtt tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt
      421 actgtggtcg acaaccagca catgcttttg cgccatcgac atggcccagt gttttaccgg
      481 caactcttca agattaaaac aggtagcgcc tttactgaca tgcacgcccg ccgcggaata
      541 aaaagcgata tccgggcaaa aattattcaa cgtttgctga aaatcgatgg gtttgaaaat
      601 ggcgttgctg gcgtgaaatt caccaccgca cagaaatgcg cggcaatggg gtttctcttt
      661 cagcgccaga aaggtattta gcgaataaca aacggcggta aaagggattt cattatcaat
      721 cgcttcaata agccacggcg tggtggtgcc acagtcaaaa aagagggtct gatcgggttc
      781 taccagtgtc gcagccagtt ttgccgcccg gcgtttttct tccaccaggc gggatttttg
      841 atcgcttaac aggtaatggc tggcactgcg cggttccaga acaatatagc cgccgagcaa
      901 aacgacgggc gcactgtggt tgttcagatc gcgacgaatc gtcatctccg aaaccccaag
      961 cagggcggcg gcgtctttaa gatgtaactt atcgctgcgt tttaattctt gcagcagctg
     1021 cccgatacgc tcttcgcgac gtgtttccat aatccctctg aatagttatt gaagcgagcc
     1081 gctcaatact acacttttta gcagagatca gtcacgcacc cagcctttgc ggatcggtaa
     1141 tgcaaaacag acgcgatacc acgattgcag acgttgatag agtttatgcc ccatcgcttg
     1201 ccagataatc tgggcactaa ggcagccaat catacctgcc agcaatccac cgagcatatc
     1261 cagcggccag tggacgccaa gataaacgcg cgaccaggca atgacgacgg ccagcaccat
     1321 taaaagtgag ccggaccaca ggcgatgcca gcataaaaat gccagtgcaa aggtgaaaat
     1381 caccgtaccg tgatcgctcg ggaatgagtc atccgccgca tgatgcagga agttatagcc
     1441 gatattttcg acaaagggtc ggtcgtgcgg aaaaagatgt cccatcgtcc aggacacaaa
     1501 caggctgacc gccagcgcga tagcgatttt tatcaccagt tgccgttgtg ctgtaagccc
     1561 ccacaaccaa agtaccgcgg ccagcaacgg caccacggta atcaaatctt tagcaataaa
     1621 aatcgccaac gagatcatcc acggagccga gtctggcgtc gcgttaataa gagagaatag
     1681 agagagattc aaattttcca gcataacttc ccgacgcaaa gtgattaaaa gggagccaat
     1741 acaggcaagt cgttgagaat aaagtgcagg ttaaactggg taaagcggca tcgtcttatt
     1801 tccctcaagc ggcctgttta cggtgggtga ttgtaacggg cataggttaa ataaaactta
     1861 aagaaagcgt agctatactc gcaataatgt aagaatgtgc ttaaccgtgg tttcagctac
     1921 aaaattcgct ttctcgttag ctgcgctttt attaaactct gcgcgattat tattggcgaa
     1981 gaaattgcat gcaaaataaa ttagcttccg gtgccaggct tggacgtcag gcgttacttt
     2041 tccctctctg tctggtgctt tacgaatttt caacctatat cggcaacgat atgatccaac
     2101 ccggtatgtt ggccgtggtg gaacaatatc aggcgggcat tgattgggtt cctacttcga
     2161 tgaccgcgta tctggcgggc gggatgtttt tacaatggct cctggggccg ctgtcggatc
     2221 gtattggtcg ccgtccggtg atgctggcgg gtgtggtgtg gtttatcgtc acctgtctgg
     2281 caatattact ggcgcaaaac attgaacaat tcaccatgtt gcgcttcttg cagggcataa
     2341 gcctctgttt cattggcgct gtgggatacg ccgcaattca ggaatccttc gaagaggcgg
     2401 tttgtatcaa gatcaccgcg ctgatggtga acgtggcgct gattgctccg ctacttggtc
     2461 cgctggtggg cgcggcgtgg atccatgtgc tgccttggga ggggatgttt gttttgtttg
     2521 ccgcattggc agcgatctcc tttttcggtc tgcaacgagc tatgcctgaa accgccacgc
     2581 gtataggcga gaaactgtca ctgaaagaac tcggtcgtga ctataagctg gtgctgaaga
     2641 acggccgctt tgtggcgggg gcgctgggat tcgttagcct gccattgctg gcgtggatcg
     2701 cccagtcgcc gattatcatc attaccgggg agcagttgag cagctatgaa tatggcttgc
     2761 tgcaagtgcc tattttcggg gcgttaattg cgggtaactt gctgttagcg cgtctgacct
     2821 cgcgccgcac cgtacgttcg ctgattatta tgggcggctg gccgattatg attggtctgt
     2881 tggtcgctgc tgcggcaacg gttatctcat cgcacgcgta tttatggatg accgccgggt
     2941 taagtattta tgctttcggt attggtctgg cgaatgcggg actggtgcga ttaaccctgt
     3001 ttgccagcga tatgagtaaa ggtacggttt ctgcggcgat gggaatgctg caaatgctga
     3061 tctttaccgt cggtattgaa atcagcaaac atgcctggct gaacgggggc aacggactgt
     3121 ttaatctctt caaccttgtc aacggaattt tgtggctgtc gctgatggtt atctttttaa
     3181 aagataaaca gatgggaaat tctcacgaag ggtaaaaaaa tgcctgattg atttgtgcga
     3241 tcaggcattc tcgaattaat ggtgatggtc gtcaatctgg tgttcgataa ccatcccttc
     3301 acctacgctg gcaagatggc gaacataagg atgcgggcgg taagccggag ctggcacagg
     3361 agccacatag acggtttgcg gcacagtcgc gacgctgaca cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattggagc ctgcccggta ttttgccgct
     3721 gcgacgaccg cgctgccggc atttttcatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcactacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatactc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa cggtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgccctgg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaagcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggtgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atggtaattt acgataatgt gttttaccag aattcagggt gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt tttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggtaaaa cgtcttgccc cacagctgat gaaccgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc tccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcggct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg gcctggggat gagttttttt
    11341 gaatgtaata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 cttgaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggtgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gcgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctag ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagcagtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcac agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcctggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgctgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggcaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca ccaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag ttagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaatc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacgtg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tatgaataca aaaggtgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tggatgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatgctttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcca gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gtgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat cgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc agaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgtttgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccacct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gcaggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgccagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gtgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgtgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcgt cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaaca caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaactaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 tttcctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 caggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actgacaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaatcac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaaatagg
    36841 gtcgttttat agggtcgtcg taaaaaatga cgacggaaat aatgcgcggc tattttaaaa
    36901 acgaaggcga gtcattcacc agataaataa atccagtaaa tttgatttag ggcaacagcg
    36961 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcaagcaaaa gatttaatcc
    37021 tttaggcgta ataaaaaata atttatcatg ctaattattt gattttgttg tttttgcggg
    37081 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca attgccatgc
    37141 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
    37201 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
    37261 gcggtatcaa ccgtttgtcg ttgatgctcg aacaaaacaa cgtcctgatt ggggagaacg
    37321 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
    37381 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
    37441 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
    37501 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
    37561 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
    37621 ataaagacgg acatccgatt gatgttgagg atattaacga tcaggcacgc catctggtgc
    37681 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
    37741 cggtgccaaa tgtccccaat gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
    37801 agttgtcctc acatccgcaa aatctctctg atggacagat tcgtcaggga ctttccgcaa
    37861 tggtgcagtt gcttgagcat tatttctctg agcagggggc tggacaggcg cgatatcgtt
    37921 taatgcggcg gcgggccagc aatgagcaac gaagctggcg ctatctggac atcatcaacc
    37981 ggatgattga ccgacctgga gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
    38041 ctttattgca ggcaaaaggc acattgcgac tggataaaga cgcgcgtccg ttattgctaa
    38101 ttgaagatcc agaaacccgc ctgcatccga ttatgctttc cgttgcctgg catctgttga
    38161 atcttttgcc tttacagcgt attgctacca ccaactctgg cgagctgctt tcgttaactc
    38221 cagtggaaca tgtttgccgc ctggtgcgtg aatcttctcg tgttgccgcc tggcgtctgg
    38281 ggccaagtgg cttgagcacc gaagacagcc gccgcatctc ttttcacatt cgttttaatc
    38341 gtccgtcatc gctgtttgct cgctgctggt tgctggtgga aggggaaacg gaaacctggg
    38401 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
    38461 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
    38521 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
    38581 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
    38641 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
    38701 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
    38761 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
    38821 taccaacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
    38881 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
    38941 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
    39001 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgactcc cagaacgcgt
    39061 tgtaatcagc atggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
    39121 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
    39181 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
    39241 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
    39301 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgga
    39361 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
    39421 actgctcacc gtttttgcct tccagtttag ccagttgcag cccgggggta ttcaaatata
    39481 agctgaattc ttccgccgac atacacccgc ggagtaacgc ataatggtaa cgtaacgcct
    39541 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
    39601 gtcgcggctg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
    39661 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
    39721 agcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
    39781 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
    39841 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
    39901 tgtgattact aacgtaaatt ttagggtttt gttgatattt cgttgaagtt aatgacccgg
    39961 attggcatgt ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
    40021 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
    40081 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
    40141 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgta ggcgcgcagg tcagcggtca
    40201 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
    40261 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
    40321 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
    40381 tcagcaacgt ctggcacaaa cgcaagcggt ttcactgcag gatctcgata ccgccgcgac
    40441 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
    40501 ggcctctctc gatacggcta aaaccaatct cgattacacc cgtatcgttg ccccgatggg
    40561 cggggaagtg acgcaaatca ccactctgca aggccagacg gtgattgccg cacagcaagc
    40621 accgaacatt ctgacgctgg cagatatgag cactatgctg gtaaaagcgc aggtttctga
    40681 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtgc ttggcgatcc
    40741 actgacgcgc tacgaggggc aaatcaagga tgtgctaccg acgccggaaa aggtaaacga
    40801 cgctattttc tattacgccc gttttgaagt acccaacccc aatggtttgc tgcggctgga
    40861 tatgaccgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgttga cgatccctct
    40921 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
    40981 tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
    41041 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
    41101 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg tgatgagcag
    41161 gttgaggtgc tgaagggcat cagcctcaat atttatgcgg gtgagatggt cgcgattgtt
    41221 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
    41281 accagcggca cctatcgcgt agccggtcag gatattgcca cgctggacgc cgatgcgctg
    41341 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
    41401 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ccggtcttga gcggaaacag
    41461 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
    41521 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
    41581 aacggcggtc aggtaattct tgccgatgaa ccgaccggcg cactggacag ccattctggc
    41641 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
    41701 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
    41761 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
    41821 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
    41881 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
    41941 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
    42001 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
    42061 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
    42121 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
    42181 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
    42241 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
    42301 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
    42361 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
    42421 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
    42481 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
    42541 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
    42601 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
    42661 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg tattggtgta
    42721 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
    42781 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
    42841 ctggtcggtg gcgcgttggg aataacactg tcactgttaa ttgctttcac cttgcagctt
    42901 ttcttacccg gctgggagat tggtttttca ccgttggcgc tgctgctggc gtttctctgc
    42961 tcgacggcca ccgggatttt atttggctgg ttacccgcac gaaatgcggc acgactggat
    43021 ccagtagatg ctctggcacg ggagtaattt ttgagataaa aatgccagcc gatcgggctg
    43081 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
    43141 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
    43201 aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg aataatgagc
    43261 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaacctttgg cattgttgaa
    43321 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
    43381 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
    43441 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
    43501 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
    43561 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
    43621 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
    43681 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
    43741 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
    43801 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
    43861 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
    43921 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
    43981 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
    44041 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
    44101 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
    44161 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
    44221 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
    44281 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
    44341 tctggtcgct atcttcagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
    44401 agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag acgagccgac
    44461 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
    44521 tatggagaat ttcacgacga acctgaacca gcttgcgcgt gtgggcggaa tcgacccact
    44581 aattggtcgt gagaaagagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
    44641 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcag aaggtcttgc
    44701 ctggcgaatt gttcagggtg atgtgccgga agtgatggct gactgtacga tttactctct
    44761 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
    44821 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
    44881 caccattatc ggtgcgggtg cagcgtctgg tggtcaggtc gatgcggcta acctaatcaa
    44941 accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc aggagttcag
    45001 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
    45061 accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
    45121 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
    45181 cattaacgat cgtcatctgc cggataaagc cattgatgtt atcgacgaag cgggcgctcg
    45241 cgcacgtctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
    45301 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
    45361 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggccaggata aagccattga
    45421 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
    45481 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
    45541 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
    45601 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
    45661 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
    45721 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
    45781 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ctaacgccgg
    45841 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
    45901 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
    45961 ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat tcatcgtcga
    46021 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
    46081 ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg cgcgtgtcat
    46141 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
    46201 cggtcaggtg acggttgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
    46261 tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgtcaggtag gttggtgaag
    46321 tccgtaatct cgtcaggggt tacggacttt ttatttatgg ggtggaacgg tgaaaaccct
    46381 atttttggag gtgaaggtaa gttgttgata attagtgctg ctggaaggta aggataaaaa
    46441 agggtgctgc aggagaatgg gatggttttg ctttattaac aacgggctaa acgtgtagta
    46501 tttgagttca ctgccgtaca ggcagcttag aaatcgacgg ggtgcggtaa aacctttgcg
    46561 aacgcgttca ctgccgtaca ggcagcttag aaattcacag gtaacatact ccacccgccc
    46621 accatgttca ctgccgtaca gacagataaa atgcgaaaaa aaagctcgca ctttcgtacg
    46681 agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata
    46741 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctacc
    46801 aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac ggtcaagaag
    46861 aattttaatg ataattattg tttgctcata ctgtaaacaa cttgtgcagt atatctacat
    46921 cgagacaagt tatggactta tacttccaaa gtacttcata catatcacaa aataaaaagg
    46981 ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca
    47041 gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt caacagtcac
    47101 tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt
    47161 aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg tttcaagaac
    47221 ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg tatcactacc
    47281 gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta
    47341 aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc cgcaccaaaa
    47401 gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc
    47461 ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata acactctggg
    47521 gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcaccgtgg tacccagaaa
    47581 ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatc ccggcgggga
    47641 atttcgcagg caccaagcga tgctgtgtga tcgttaagga cctggcagtc gataagctta
    47701 ccgccatgac cgataaattc ctcacagaat accagaagcg ccgttttaga cgcattttcc
    47761 atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc gtacataccg
    47821 ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag ttcgtgaagg
    47881 cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg atcgctggca
    47941 cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta
    48001 tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac cgcgcgggga
    48061 tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat accacgctgg
    48121 taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag gccgttaggc
    48181 tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag ctgaaccagg
    48241 cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac aaaccctgct
    48301 tgaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct tgctcaataa
    48361 tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga agtcgatggg
    48421 tgaccattaa caccgttttc tcacgcatca tttttgcaag caattcaagg atctggcttt
    48481 cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc gcatcatgta
    48541 acagcgcacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg cgtccgcctt
    48601 cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca acgcgacgca
    48661 agatctccga cagagcctca tcactactgc caggcgaggc gagtaacaga ttatcacgca
    48721 gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt cgtagagccg
    48781 cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc
    48841 atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc ccgagaatcg
    48901 ctatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt tgggattgct
    48961 ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca acacgagttt
    49021 gggtatcagg gaaggtgacc tccggttttt gatccgttaa gtcagagata cgtacggcag
    49081 aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt gcttcaaacg
    49141 cggctaacgc acaaaagaca aacagggcaa ttaatgcgcc gggttgagca ttgccgccaa
    49201 cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg agcagcatta
    49261 tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc cattgaattt
    49321 ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc agctcagctt
    49381 gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga gtcagatttt
    49441 gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc gtcagtaaca
    49501 taacgctgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg attgtcacca
    49561 ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa agatgatcga
    49621 gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag cgcgccagtc
    49681 cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc
    49741 ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta cgggtgattg
    49801 cggcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt ccggcaaccc
    49861 ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg atactggcga
    49921 gctgcgtcac aattgccagc acaataccaa gacttaacat ccatttatga cgtttataca
    49981 gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg
    50041 tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca ataatccggc
    50101 catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg tgggtgacca
    50161 ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc tgttcactgt
    50221 gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga tttagtaacg
    50281 cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa
    50341 caggtgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag gcgttatcca
    50401 gcgctgcttg taattcttgt tcgctggcat caggtcgcgc cagtagtacg ttatcccgca
    50461 attttgctgc cggtaattgt gggttttgcc caacccagga gagatgttta cgccatgatt
    50521 ctggtgataa atcacgtaat tctatcccgt tgattcgtaa tgatccctga tatgagagaa
    50581 aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcggcca accaacactg
    50641 cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta ccttccggcg
    50701 acgtgataaa cagatcctcg gcctcaatgg tcagcggatc ggtcaatgct aattccgcct
    50761 caccgcgctg cggatgggcg agcggggttt ccataaacgt tttcagactg tcagctgcac
    50821 caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc tggaaaaact
    50881 ctggcgcaag gatcagggcc agaaaacctg cagccagcgt caccccagta tcgtagtgac
    50941 caaaatccag ctcgccgaga taggaaaaac caaagtagac cgccaccaga gcaattgaca
    51001 gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagtcgtagc acttccattg
    51061 tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct tcaccacgac
    51121 caaaaatacg caatgtttcc atgccgcgca ggcgatcgag gaaatgccca cttaagcgag
    51181 caagagcgag aaagttacgc cggttagcat cggcagcccc cattccaacc agcgccataa
    51241 acaacggaat tagcggtgct gtgcccagca gaatgagcgc cgcagcccag ttagagggga
    51301 agatcgccac cacaatcagc aacggcaccg acactgccag cgccatttgc ggcagatagc
    51361 gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg
    51421 caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga acctgacggc
    51481 ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt aaccagacca
    51541 cccatgcgcg caatacaaag gtcagaacca gtaacgtaaa gggaagcagc agggcttcac
    51601 ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccag gcctgggcaa
    51661 tgatcaatat gccgcttaca aagcccagca gacgagaaat attcagccaa cgttgggaga
    51721 tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta ttcattgcac
    51781 gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg gaaaaaataa
    51841 aggcgaccca tagtcgcaag gtgtcgcctt ctttactttt gttactgatt tgtaaaatta
    51901 ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca tgcagccagt
    51961 accggccgaa gtaatggcct ggcgataaat gtgatccatc acgtcgcctg cggcaaagac
    52021 gccaggaatg ctggtctggg tggcattacc atgaataccc gactgtactt tgatgtagcc
    52081 gttttccagt tccagctgcc cttcgaaaat cgcagtattc gggctgtgac cgatagcaac
    52141 aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg
    52201 aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtacggt tggtgtgcag
    52261 aatgatgttg ccgttctcca ctttatccat cagacgctta ataaggattt tttccgcgcg
    52321 gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg atgttagaca gatacagcgc
    52381 ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc gatagaagaa
    52441 accgtcgcag gttgcacaag cagaaacccc acggccttta aaggcttctt cagagggcag
    52501 gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc
    52561 gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga tatgatcaaa
    52621 aatgatctca gtttcaaact tggtggcatg ttcgtgcatg cgcgccatta ataacggacc
    52681 ggtcagatcg tttggatcac caggccagtt ttccacttcc gtggtggtgg tcagttggcc
    52741 gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg cgtagacagc
    52801 agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc
    52861 catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat ttacagacgt
    52921 aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg
    52981 tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta
    53041 aatgatgaat aaacgcccct gttaatgaat atctggcatg ttgtactaaa aattgatgtt
    53101 ttgctttgac aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt
    53161 cttatatgcg cataaccatg tatgtaaata ccatgtttac cgtgctagtg aaatctacgt
    53221 atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca
    53281 ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta
    53341 gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac
    53401 aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa
    53461 cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt
    53521 gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat
    53581 tcagggctat acggcgctgc ttaaccccca ttatctggat gcatcacttc tggtattcgt
    53641 tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca
    53701 aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact acctgttgaa
    53761 aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc tgctgcgtct
    53821 gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg
    53881 tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac
    53941 tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca
    54001 tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac
    54061 aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc
    54121 cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac
    54181 cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt
    54241 gcgtggttgg cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc
    54301 attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ccagcgacga atacattgat
    54361 tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt
    54421 ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc
    54481 ttactaagca ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc
    54541 tgcgtttggg cagcgggcct gacgttgttc accggttggt catgggtgac cattgctgaa
    54601 aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat
    54661 gatacctggg tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac
    54721 ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa
    54781 cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc gttgttctcc
    54841 ggtaagcgga tggatgatga cgaagagatt acctacactg cacgcggtgt ggctgccgac
    54901 ccggacgacg tcctattttt gggcaatcgt gcaacgcagc cagaatatga cgaatacgat
    54961 ccattattaa acggtgcgcc aattaccgaa cctgtcgctg tagcagctgc tgctaccacg
    55021 gcgacacaaa gctgggctgc gccggttgaa cctgtgactc agacgccgcc tgttgcctct
    55081 gttgatgttc caccttcgca acctacagta gcctggcagc ctgtaccggg tccacaaacg
    55141 ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagtcaca atatgcgcag
    55201 cctgcagtgc aatataatga gccgctgcaa caaccagtac agccgcagca gccgtattat
    55261 gcacctgcag ctgaacaacc tgcgcaacaa ccttactacg ctcctgcggc ggaacaacct
    55321 gttcagcaac cgtattacgc aactgcgccg gaacagcctg cgcaacagcc gtattatgcc
    55381 cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccatt
    55441 tttgctccac agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag
    55501 ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt
    55561 gtagaagaga caaaacccgc gcgtccgccg ctttactatt ttgaagaagt ggaagagaag
    55621 cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa
    55681 gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta
    55741 gaagccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg
    55801 ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt
    55861 ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca
    55921 actcgtcgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa
    55981 aaagcccgtg aagcccggcg caatcagtat gattctggcg atcagtataa cgatgatgaa
    56041 atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc
    56101 tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca
    56161 gaggctgaac tggctcgtca gtttacgcaa actcaacaac aacgttattc cggcgaacaa
    56221 ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg
    56281 ctggatgatg gtccacacga accgctgttt acgccaattg ttgaacctgt acagcagccg
    56341 caacaaccgg ttgcaccaca gcagcagtat cagcagccac aacagccggt tgcgccacag
    56401 ccgcagtatc agcagccaca acagcaggtt gcgccgcagc cacaatatca gcagccgcaa
    56461 caaccggttg cgccacagca gcaatatcag cagccgcaac aaccagttgc gccgcagccg
    56521 cagtatcaac agccgcaaca accggttgca ccgcagccgc agtatcagca gccacaacag
    56581 ccagttgcgc cacagccgca ggataccctg cttcatccgc tgttgatgcg taatggcgac
    56641 agccgtccgt tgcacaaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg
    56701 ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggcgcg tctggtggaa
    56761 gcgcgtctgg ctgatttccg tattaaagcc gatgtcgtta attactctcc ggggccagtt
    56821 atcactcgct ttgaacttaa cctggctcct ggcgtaaaag cggcgcgcat ttctaacttg
    56881 tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct
    56941 ggcaaaccct atgtaggtct ggagttacca aataaaaaac gacaaaccgt ttatctgcgc
    57001 gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt ggtgctgggt
    57061 aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttggtt
    57121 gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt
    57181 tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt
    57241 tcggtttatg aaggcattcc gcacctgtta acggaagtcg ttactgatat gaaagatgcc
    57301 gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc ggtataaact gatgtctgcg
    57361 ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg
    57421 cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg
    57481 ctgaaaaaag aaccatacat tgtcgtgttg gttgacgaat ttgccgacct gatgatgacg
    57541 gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt
    57601 atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa
    57661 gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt
    57721 cttgatcagg ctggcgcgga atcactgctg ggtatggggg atatgctcta ctctgggccg
    57781 aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc
    57841 gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacctccgac
    57901 agcgaaagcg aaggtggtgc gggtggtttc gatggcgctg aagaactgga tccgttgttc
    57961 gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt
    58021 cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg
    58081 attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc gccgtttgac
    58141 taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct atcaactgaa
    58201 aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga
    58261 actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg ctatcacctg
    58321 tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa
    58381 aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg
    58441 tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt
    58501 caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg
    58561 gttctataac ccgttcgttg agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa
    58621 tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa
    58681 acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt
    58741 caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga
    58801 tcagcgcagc agttatcagc tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt
    58861 taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag
    58921 tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc
    58981 ggccagaaaa tttagcacag tatatcggtc agcaacattt gctggctgcg gggaagccgt
    59041 tgccgcgcgc tatcgaagcc gggcatttgc attctatgat cctctggggg ccaccgggta
    59101 ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta
    59161 tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa
    59221 accgcaatgc aggtcgccgc actattcttt ttgttgacga agttcaccgt ttcaacaaaa
    59281 gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa
    59341 ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtttatc
    59401 tgttgaaatc cctgagtaca gaggatattg agcaagtact aactcaggcg atggaagaca
    59461 aaacccgtgg ctatggtggt caggatattg ttctgccaga tgatacacga cgtgccattg
    59521 cagaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata
    59581 tggccgaagt ctatgatagc ggtaagcggg tcctgaagcc tgaattactg accgaaatcg
    59641 ccggtgaacg tagcgcccgc tttgataaca aaggcgatcg cttttacgat ctaatttccg
    59701 cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctgtactgg tatgcgcgaa
    59761 ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ctgtctggcg attgcgtctg
    59821 aagacgtcgg taatgccgat ccacgggcga tgcaggtggc aattgcggcc tgggattgct
    59881 ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg
    59941 cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agccgcgctg gccgatgctc
    60001 gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga
    60061 aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg
    60121 ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc ccgacaaaca
    60181 ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata
    60241 gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt
    60301 ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg
    60361 atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct
    60421 ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca
    60481 aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag
    60541 cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg
    60601 atgcagcaaa agccgagcta gatgctttac aggctgaaat tcgcgatatc gcgctgacca
    60661 tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag
    60721 tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg
    60781 gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg
    60841 tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc
    60901 tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca
    60961 cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat actcgtccgc
    61021 tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa gttccgctga
    61081 ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc
    61141 acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc
    61201 gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccagaa gactcaatgg
    61261 cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt
    61321 accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa acttacgacc
    61381 tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc tccaacgttt
    61441 gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac aagaaaaccc
    61501 gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa
    61561 tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg cgtccgtata
    61621 tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa agcgccttta
    61681 gggcgctttt tttgtctccc tttgataccg aacaataatt actcctcact tacacgtaat
    61741 actactttcg agtgaaaatc tacctatctc tttgattttc aaattattcg atgtatacaa
    61801 gcctatatag cgagctgcta tagaaataat tacacaatac ggtttgttac tggaatcaat
    61861 cgtgatcaag cttgagtgag ccattatgaa aacgaaaatc cctgatgcgg tattggctgc
    61921 tgaggagagt cgccgtggtt tggtaaaaac gacagcgatc ggcggcctgg caatggccag
    61981 cagcgcatta acattacctt ttagtcggat tgcgcacgct gtcgatagcg ccattccaac
    62041 aaaatcagac gaaaaggtta tctggagcgc ctgtacagtt aactgtggta gtcgctgccc
    62101 gctacgtatg cacgtcgtgg acggtgaaat caaatatgtc gaaacggaca ataccggcga
    62161 tgacaattac gacggcctgc actaggttcg cgcctgcctg cgtgggcgtt ccatgcgtcg
    62221 ccgtgtctac aatccggacc gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga
    62281 aggcaaattc gagcgcatta gctgggaaga agcctacgac atcatcgcga ccaatatgca
    62341 gcgcctgatc aaagagtacg gcaacgagtc tatctatctg aactatggca ccggtacgct
    62401 gggcggcacc atgacccgct cctggccgcc gggaaatacc ctggtcgcgc gcctgatgaa
    62461 ctgctgcggc ggttatctta accattacgg tgactactct tccgcgcaaa ttgcggaagg
    62521 tttgaaccat acctacggcg gctgggcaga tggtaacagc ccgtcggata tcgaaaacag
    62581 taaattggta gtgctgtttg gtaataaccc aggcgaaacg cgaatgagtg gcggtggggt
    62641 gacttactat cttgaacagg cacgccagaa atctaatgct cgcataatca tcatcgatcc
    62701 gcgctatacc gacaccggtg ccgggcgcga agatgagtgg atccctattc gtccaggaac
    62761 agatgccgca ctggttaacg gtctggcgta cgtcatgatc actgaaaacc tggtggatca
    62821 ggcattcctc gataaatatt gcgttggcta tgacgagaaa accctgcccg ccagtgcgcc
    62881 gaaaaatggt cattataaag cttatattct gggtgaaggg ccagatggcg tggctaaaac
    62941 accggaatag gcctcgcaaa tcaccggtgt tccggcagac aaaatcatca aactggctcg
    63001 tgaaatcggc agtaccaaac cggcgtttat cagccaggga tggggcccgc agcgtcacgc
    63061 taacggtgaa atcgcaaccc gtgctatctc gatgctggcg attctgaccg gtaacgttgg
    63121 tattaatgga gggaacagcg gcgcgcgtga aggttcatac agcttaccgt ttgtccgtat
    63181 gccgaccttg gaaaacccga tccagaccag catttcgatg tttatgtgga ccgatgccat
    63241 tgaacgtggc ccggaaatga cggcgctgcg tgatggtgtg cgcgggaaag ataagctgga
    63301 tgtgccgatc aaaatgatct ggaactatgc cggtaactgc ctgattaacc agcattctga
    63361 aatcaaccgt acccatgaaa tcctccagga tgataagaag tgcgagctga ttgtggttat
    63421 cgactgccac atgacctcat cggcgaaata tgctgacatc ctgctgcctg actgcaccgc
    63481 ttccgaacag atggactttg cactggatgc atcctgcggg aatatgtctt acgtgatttt
    63541 caacgatcag gtgattaaac cgcgctttga atgtaagacc atctatgaaa tgaccagcga
    63601 actggcaaaa cgtcttggcg ttgagcaaca gtttactgaa ggccgtaccc aggaagagtg
    63661 gatgcggcat ctgtatgccc agtcgcggga agcgattcct gaactgccaa cgtttgaaga
    63721 gttccgcaag caggggatct ttaaaaagcg cgacccacaa gggcatcacg ttgcttataa
    63781 agccttccgt gaagatccgc aggcaaaccc actgactacg ccatcgggca aaattgagat
    63841 ttattcgcag gcgctggctg acattgccgc tacctgggaa ttgccagaag gcgatgtgat
    63901 cgatccactg ccgatctaca cgccgggctt tgaaagttat caggatccgc tgaacaaaca
    63961 gtatccgctg cagcttacag gtttccacta taaatctcgc gttcactcaa cttacggcaa
    64021 cgttgatgtg ctgaaagcgg cttgccgtca ggaaatgtgg atcaacccgc ttgatgccca
    64081 aaaacgcggt atccacaacg gcgataaagt caggatcttt aacgatcgtg gtgaggttca
    64141 tattgaggcg aaagtgacgc cacgaatgat gccgggcgtg gtcgcactgg gtgaaggcgc
    64201 ctggtatgac ccggatgcaa aacgtgtcga taagggcggt tgtatcaacg tactgaccac
    64261 tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat acaaaccttg ttcaggttga
    64321 aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt ttttattgat tccagccgtt
    64381 gcaccggttg caaaacctgc gagctggctt gtaaagacta caaagatttg acgccagaag
    64441 tcagtttccg ccgcatttat gaatatgctg gcggcgactg gcaggaagat aacggtgtct
    64501 ggcaccagaa cgtgtttgcc tactatctgt cgatttcgtg caaccactgc gaagatccgg
    64561 cttgtactaa agtctgcccg agcggtgcga tgcataaacg cgaagatggt tttgttgtgg
    64621 tcgatgaaga tgtatgcatt ggctgccatt actgtcatat ggcttgcccg tatggcgcac
    64681 cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga tggttgttat gaccgtgttg
    64741 cggagggtaa aaagccgatc tgtgttgaat cttgcccgct gcgggcgctg gatttcggcc
    64801 ctatcgacga gctgcgtaaa aaacatggcg atctggcggc cgttgcgccg ttgccgcgag
    64861 ctcactttac caaaccgaat attgtgatca aacccaatgc caatagccgc ccgacagggg
    64921 ataccactgg ctatctggca aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa
    64981 tggccgctga tgatcttcac ggtcttcggg caatgtgtgg cgggtggttt tatcgttctg
    65041 gctttggcgc tgctcaaagg cgacctgcga gcagaagccc agcagcgtgt tatcgcctgc
    65101 atgtttggct tatgggtact gatgggcatt ggctttatcg cctctatgct ccatcttggt
    65161 tcaccgatgc gcgcctttaa ctcgctcaac cgggtagggg cttcagcact cagtaacgaa
    65221 atcgccagcg gttcgatctt ttttgctgta ggcggcatcg gctggttgct ggcaatgctg
    65281 aaaaagctgt caccggcatt gcgtacgcta tggctggtgg tgacgatggt tcttggcgtc
    65341 gtctttgtct ggatgatggt gcgtgtgtat aacagcattg ataccgttcc gacctggtac
    65401 agcatctgga cgccgatggg cttcttcctg acgatgttca tgggcggccc gttactgggt
    65461 tacctgttgc tgagtctggc tggcgtcgat ggctgggcga tgcgtctgct gccagccatt
    65521 tctgtactgg cactggtggt gagtggcgtg atgtcagtga tgcagggcac agagctggca
    65581 actattcata gttctgtgca gcaggccgca gcgctggtgc cggactatgg tgcgctgatg
    65641 tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc acagctaaaa
    65701 ggttatcagc ctgcggttcc gctactatcc gtctcattca ttctgcttct ggcaggggaa
    65761 ttaatcggtc gcggcgtatt ctatggtttg catatgaccg tggggatggc cgtcgcaagc
    65821 taatcagtaa caaccggggt ttcggccccg gttctctttt atttctgctt cgttaacgtg
    65881 tcataactgg tcatcaaatt acgataatca ggaatatggt tagagaacaa cgtcgccagt
    65941 ccttcaatat cattacgcca gtcacggtgc agctcgcaag ccacgccgaa ccatgtcatc
    66001 aattgcgcac cagcttgcga catgcgatcc cacgccgaat gccgggtaat ttcattaaag
    66061 gtccctgatg catcagtcac gacaaagaca tcaaaccctt cttcaatggc tgaaagcgcc
    66121 gggaatgcca cgcaaacttc ggttaccaca ccggcaataa ttaactgttt tttacctgtc
    66181 gctttgactg cttttacaaa atcttcgtta tcccaggcgt taatattgcc cgggcgagca
    66241 atgtaaggtg catcggggaa ttgtgctttc agttctagaa ctaatgggcc gttaggaccg
    66301 gtttcaaaac tggtggtaag aatagttggt aagttgaaat acttagccag gtcacccagc
    66361 gccagcacgt tatttttaaa cttatcgggt tcgatatccc gtacaaggga aagtaaacca
    66421 gcctggtgat caacaagcaa aacggcagca tcatttttat caagacgaac atacggtttg
    66481 gtcatcctct tctcctttcg agagtcagtg gtacgtctga aatcggtcag acgcgatgca
    66541 ttgctctgaa agcatagacg ggaaatatga gtttgctgtg accatgaaat ttttcgactg
    66601 aacgaagcgt atgaaatttg tgttagatca ataaaaacaa tcagatgaga ttaatcatct
    66661 cttcagcatt agcgttatga atgagtaata taaatcgata ataccgccgc tgcgatatgc
    66721 gcgtattttc acccattgac aatgtttttg gcggtggcat gatgcgcatg aaatttgaac
    66781 ttcctcacgg ttttaattca tgtccacgta tacccggcct gtcatgcttt tactgtctgg
    66841 cctgcttttg ttgactctgg cgatcgcggt gttaaataca ctcgtgccgc tttggctcgc
    66901 ccaggaacac atgtccacat ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa
    66961 ccttgtcggt acattgctga cagggtatgt cattaaacgc attggcttta accgcagcta
    67021 ttatctgtcc tccttcattt ttgctgctgg ctgtgccggc cttggcctga tgattggttt
    67081 ctggagctgg ttggcgtggc gttttgtcgc gggcgtcggc tgtgccatga tttgggtggt
    67141 tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc
    67201 gtatatgatg gtttattacg tgggaacgtt tttaggccag ttactggtca gcaaagtctc
    67261 aaccgagctg atgagcgtat tgccgtgggt tacaggtttg acattggcag ggatcttacc
    67321 gctgttgttt acgcgtgtcc tgaatcagca ggctgaaaac catgattcga cgtcaattac
    67381 ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat ggctgcatta tctcaggaat
    67441 cgttctggga tctctatatg gcctgatgcc tctgtacctc aatcacaaag gggtgagcaa
    67501 tgccagtatt ggtttctgga tggcggtact ggtcagtgcg ggtatcctcg gacaatggcc
    67561 gattggacgt ctggcggata agtttggtcg actgctggtt ttgcgtgttc aggtctttgt
    67621 cgtcattctc ggcagtattg cgatgcttag ccaggcggcg atggccccag cgttattcat
    67681 cctcggtgcc gctggcttta cgctatatcc ggtggcgatg gcctgggctt gtgagaaagt
    67741 tgaacatcat caactggtgg cgatgaacca agccttactg ttgagctata ccgtgggaag
    67801 tctgcttggc ccgtcattta ccgctatgct aatgcagaat ttctccgata atttattgtt
    67861 tatcatgatc gccagcgtat cgtttatcta tttgctgatg ctgcttcgca acgccggtca
    67921 tacgccgaaa cccgttgctc acgtgtaaat gaattcaagc aaagtgtgaa cttactgttt
    67981 cacactctgc ttttttgttt cttctatctg acttgctttt ttccaaattt tattcgttta
    68041 aaaataaaat gtgcagcagg ttataatttt gcatttcgct atttccgcac ttcttatttg
    68101 ccgcgcataa tccctcgttt tacagatgcc cctttaattt tggcgaagga tttgtctatg
    68161 gctgggaatg ttcaggaagt aa
//
LOCUS       DAJTXY010000007        66598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTXY010000007 DAJTXY010000000
VERSION     DAJTXY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.007"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO566_000551"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO566_000551"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6603957.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO566_000552"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO566_000552"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6603958.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO566_000553"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO566_000553"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6603959.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO566_000554"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO566_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6603960.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO566_000555"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO566_000555"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6603961.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDASHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO566_000556"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO566_000556"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6603962.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO566_000557"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO566_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6603963.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO566_000558"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO566_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6603964.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMDICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLN"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO566_000559"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO566_000559"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6603965.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO566_000560"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO566_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6603966.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO566_000561"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO566_000561"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6603967.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO566_000562"
     CDS             complement(15665..16702)
                     /locus_tag="OO566_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6603968.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO566_000563"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO566_000563"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6603969.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19304)
                     /gene="ggt"
                     /locus_tag="OO566_000564"
                     /pseudo
     CDS             complement(17582..19304)
                     /gene="ggt"
                     /locus_tag="OO566_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            19424..19864
                     /locus_tag="OO566_000565"
     CDS             19424..19864
                     /locus_tag="OO566_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6603970.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="OO566_000566"
     CDS             complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="OO566_000566"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6603971.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20591..21581)
                     /locus_tag="OO566_000567"
                     /pseudo
     CDS             complement(20591..21581)
                     /locus_tag="OO566_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="OO566_000568"
     CDS             complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="OO566_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6603972.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="OO566_000569"
     CDS             complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="OO566_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6603973.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="OO566_000570"
     CDS             complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="OO566_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6603974.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25123..25836)
                     /gene="livF"
                     /locus_tag="OO566_000571"
     CDS             complement(25123..25836)
                     /gene="livF"
                     /locus_tag="OO566_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6603975.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSSYLGG"
     gene            complement(25838..26605)
                     /gene="livG"
                     /locus_tag="OO566_000572"
     CDS             complement(25838..26605)
                     /gene="livG"
                     /locus_tag="OO566_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6603976.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26602..27879)
                     /gene="livM"
                     /locus_tag="OO566_000573"
     CDS             complement(26602..27879)
                     /gene="livM"
                     /locus_tag="OO566_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6603977.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27876..28802)
                     /gene="livH"
                     /locus_tag="OO566_000574"
     CDS             complement(27876..28802)
                     /gene="livH"
                     /locus_tag="OO566_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6603978.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28850..29959)
                     /gene="livK"
                     /locus_tag="OO566_000575"
     CDS             complement(28850..29959)
                     /gene="livK"
                     /locus_tag="OO566_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6603979.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30383..30766
                     /gene="panM"
                     /locus_tag="OO566_000576"
     CDS             30383..30766
                     /gene="panM"
                     /locus_tag="OO566_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6603980.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30965..32068)
                     /gene="livJ"
                     /locus_tag="OO566_000577"
     CDS             complement(30965..32068)
                     /gene="livJ"
                     /locus_tag="OO566_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6603981.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="OO566_000578"
     CDS             complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="OO566_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6603982.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="OO566_000579"
     CDS             complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="OO566_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6603983.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="OO566_000580"
     CDS             complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="OO566_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6603984.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="OO566_000581"
     CDS             complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="OO566_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6603985.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36806..37402
                     /gene="rsmD"
                     /locus_tag="OO566_000582"
     CDS             36806..37402
                     /gene="rsmD"
                     /locus_tag="OO566_000582"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6603986.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37392..37661
                     /locus_tag="OO566_000583"
     CDS             37392..37661
                     /locus_tag="OO566_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6603987.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37664..38023)
                     /locus_tag="OO566_000584"
     CDS             complement(37664..38023)
                     /locus_tag="OO566_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6603988.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38164..38790
                     /locus_tag="OO566_000585"
     CDS             38164..38790
                     /locus_tag="OO566_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6603989.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38864..41062
                     /gene="zntA"
                     /locus_tag="OO566_000586"
     CDS             38864..41062
                     /gene="zntA"
                     /locus_tag="OO566_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6603990.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="OO566_000587"
     CDS             complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="OO566_000587"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6603991.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41630..42295
                     /locus_tag="OO566_000588"
     CDS             41630..42295
                     /locus_tag="OO566_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6603992.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42368..42925
                     /gene="dcrB"
                     /locus_tag="OO566_000589"
     CDS             42368..42925
                     /gene="dcrB"
                     /locus_tag="OO566_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6603993.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42929..44146)
                     /locus_tag="OO566_000590"
                     /pseudo
     CDS             complement(42929..44146)
                     /locus_tag="OO566_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44278..45327
                     /locus_tag="OO566_000591"
     CDS             44278..45327
                     /locus_tag="OO566_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6603994.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45382..45969
                     /gene="acpT"
                     /locus_tag="OO566_000592"
     CDS             45382..45969
                     /gene="acpT"
                     /locus_tag="OO566_000592"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6603995.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46080..47654
                     /gene="nikA"
                     /locus_tag="OO566_000593"
     CDS             46080..47654
                     /gene="nikA"
                     /locus_tag="OO566_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6603996.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47654..48598
                     /gene="nikB"
                     /locus_tag="OO566_000594"
     CDS             47654..48598
                     /gene="nikB"
                     /locus_tag="OO566_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6603997.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48595..49428
                     /gene="nikC"
                     /locus_tag="OO566_000595"
     CDS             48595..49428
                     /gene="nikC"
                     /locus_tag="OO566_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6603998.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLILGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49428..50192
                     /gene="nikD"
                     /locus_tag="OO566_000596"
     CDS             49428..50192
                     /gene="nikD"
                     /locus_tag="OO566_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6603999.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50189..50995
                     /gene="nikE"
                     /locus_tag="OO566_000597"
     CDS             50189..50995
                     /gene="nikE"
                     /locus_tag="OO566_000597"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6604000.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51001..51402
                     /gene="nikR"
                     /locus_tag="OO566_000598"
     CDS             51001..51402
                     /gene="nikR"
                     /locus_tag="OO566_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6604001.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51522..51881)
                     /locus_tag="OO566_000599"
     CDS             complement(51522..51881)
                     /locus_tag="OO566_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6604002.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51878..52153)
                     /locus_tag="OO566_000600"
     CDS             complement(51878..52153)
                     /locus_tag="OO566_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6604003.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52212..53336)
                     /locus_tag="OO566_000601"
     CDS             complement(52212..53336)
                     /locus_tag="OO566_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6604004.1"
                     /translation="MRQLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="OO566_000602"
     CDS             complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="OO566_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6604005.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56068..57135)
                     /locus_tag="OO566_000603"
     CDS             complement(56068..57135)
                     /locus_tag="OO566_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6604006.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57501..59123)
                     /locus_tag="OO566_000604"
     CDS             complement(57501..59123)
                     /locus_tag="OO566_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6604007.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINKNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59385..60974)
                     /locus_tag="OO566_000605"
     CDS             complement(59385..60974)
                     /locus_tag="OO566_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6604008.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEESQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61358..62389
                     /locus_tag="OO566_000606"
                     /pseudo
     CDS             61358..62389
                     /locus_tag="OO566_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62923..63687)
                     /locus_tag="OO566_000607"
     CDS             complement(62923..63687)
                     /locus_tag="OO566_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6604009.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSANLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNGETYAICSEVLLYD"
     gene            63934..64695
                     /locus_tag="OO566_000608"
     CDS             63934..64695
                     /locus_tag="OO566_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6604010.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSLQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64802..65302)
                     /locus_tag="OO566_000609"
     CDS             complement(64802..65302)
                     /locus_tag="OO566_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604011.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65453..>66598)
                     /locus_tag="OO566_000610"
     CDS             complement(65453..>66598)
                     /locus_tag="OO566_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6604012.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDQK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16175 a        17872 c        16342 g        16209 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgtaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaagtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgccgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgagg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta attcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagtag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatgtcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgaga ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cgcccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgccgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc taaaacaact tcctgagagc
    19261 agagcagcaa tggccacccg gcgtaaaaac gtcggtttta tcatcgttat tctccagaga
    19321 ttaaggggca accccagcta agcctggtat ataactctga attaatcatc gttttgtcgg
    19381 gaagcgagta aacttaaagg atatcttcag aggaggatac gcgatgaaac gacttctgat
    19441 tcttacggca ctcctgccgt ttgtcggctt tgcacagccc attaatactc tgaacaaccc
    19501 taaccagccg gggtatcaga tacccagcca gcagcggatg caaacccaga tgcagactca
    19561 gcaaatccag caaaaaggga tgctgaatca gcaactgaaa acgcaaactc agttgcaaca
    19621 gcagcattta gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt cacagccggg
    19681 ggagcgaaat cccgcccggc agcaaatgct gcccaacacc aacggcggga tgttaaacag
    19741 caaccgtaat ccggatagtt cgttgaatca gcagcatatg ctgccggaga ggagaaacgg
    19801 cgacatgctg aatcagccca gcacgccgca gcctgacatt ccgttgaaaa ctattgggcc
    19861 gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga tgcaatccac gccccagcgc
    19921 agcaactctg ccgcgcgctg gggtttattg acggtataaa ccagaatccg taatccggcg
    19981 tctttcaact gcatcactcg cgctttatcg agtaacttat gattgagatg aatagagacg
    20041 cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag caataaacca
    20101 cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag catcaatctc aaacgatgac
    20161 agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac cattttgccc
    20221 gttaatggtc cagtgccagt ggtgggtttg atttcgatat tcgccatcat cccgtgttcg
    20281 cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct cacctttaaa cgctttgctg
    20341 taccaactgc ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc cgcgacgccc
    20401 cagccgttgc tggtgcgttc gagattgtcg tcatggagca ggaagatctc gccatctttc
    20461 gataacttcg cgtcaaattc gatcatctta tgaccgtatt ttgccccgac gtcgattgcc
    20521 gccagggtgt tttccggggc cagcttaccg ccgccacgat gagcgacgat gcggggataa
    20581 ggccagttac tcatactcgt tgtcctgttt caccatcaaa aagatgcagc tgattttccg
    20641 ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc agtcgcacca
    20701 ccagcttctg cttgcgagct tagcgcaata tgttccgggc gaatgccgag ggtcatttta
    20761 cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc gtccagttcg
    20821 aaatgcgtgc cttcattatt catgcggcct gtcagcaggt tcatcgccgg gctgccgata
    20881 aaactcgcca caaacaggct ggcgggcttt tcgtagactt ccaccggcgt gccaatctgt
    20941 tcggcaacgc cgccgttcat caccattact cgctgggcga gcgtcatcgc ctcaacctga
    21001 tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcagctgttg cagttcaaga
    21061 cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg gctcatcaaa caagaacacc
    21121 gccggatcac gcacaatcgc gcgtcccatc gccacacgct ggcgctgacc gccggaaagc
    21181 tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa tgcgcgccgc ttctttaacg
    21241 cgctcggcta tttgctgctt gcccatgccg cgaattttca gcccccacgc catgttttct
    21301 tcgacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat cccgcgatct
    21361 ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc ggtcaccctc
    21421 tccagcccgg caaccatacg cagcagcgtc gatttcccgc agccagacgg cccgaccatc
    21481 acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgaccta ggttttgcca
    21541 tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct cactatcgac
    21601 caggccgcgc acgaaggcac gctgcatcac taaaacaatc actactggtg ggatcagggt
    21661 taacaacatc gccgccatca ctgagttcca ttccgtggtg ccttcgccag tggcgatcat
    21721 ccctttgatc cctgccacgg tggtgccgag atccacgtcg gtaataatca acaacggcca
    21781 caaatactgg ttccagccgt aaataaaggt gatcacaaac agcgccgcca gattagtttt
    21841 ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt gacgcaccgt cgatccgcgc
    21901 cgcttccacc agttcatccg gcagcgtcat aaagaactgg cgaaacagaa acgtggcggt
    21961 cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcatct tcaggttggc
    22021 gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg tgataaaaat
    22081 catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa atgccgagag
    22141 catcgagaca gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc tgttaagcaa
    22201 catccgccag aacggcgcgc tatttgtgcc taccccgttc acccagatgt tgtggatgtt
    22261 ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg gcgtagacgg cctgtttatc
    22321 cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga tcccgaggat
    22381 cagcatggta tggctgaata tcgtcagcca cggacggttc tcaattattg gtaacgcacc
    22441 ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa catcaacacc
    22501 accgactgtg cggcagacga agccagatcc agtccggtaa aaccttcgcg gtagatctta
    22561 taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg tggcggcgtc gatcaccggg
    22621 aaggtgtcga agaaggcata caccaggttc actaccagca ggaagaaact caccggggcg
    22681 ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc ctgcaccgtc gatggctgcg
    22741 gcttcgatca acgaacgggg aatggattgc agcgcggcat agaagaacag aaagttgtag
    22801 ctgatttgct tccacaccga ggcaaacacc accagaaaca tcgcctgacc gctattttgc
    22861 gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc gcgaccgggg
    22921 ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata cggcagcaac
    22981 attaaggttt ggtagaaacg actgccgcgc acgatgtact ccaccagcgc cgcaaagaac
    23041 agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt cgtccagaag
    23101 gagtcgagat aatagctgtc atgaaacagc gtgacgaagt tatccagacc gacaaactgg
    23161 ctggagaaac caaacggatc gacgctttgt agcgagtacc acaacgcttc gcccgcaggc
    23221 cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata aggcagccag
    23281 cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac tgtgccggat
    23341 gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac gcatccggca
    23401 ttacactgat taagacttcg tcgatttctc aaagcggcgc agtaactggt tcccacgctc
    23461 aactgcggta tccagcgcct gctgtggcgt cttcttaccg gtccacacgc tctcaagctc
    23521 ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgaagccctt tggtgaacgg
    23581 caacggcggc ttgttcagca tctgacgcgt cgcggtatcc gcacctgggt ttttctcgta
    23641 aaagccctgc tcacgggtca ggtcatacgc cgctttggta attggcagat aaccggtttt
    23701 ctgatgccac tcagcagcgt tttctggctt cgcgaggaag tcgagaaact tcgccacgcc
    23761 ggtatacgtt tctttatctt tgccctgcat cacccacagg ctggctccgc cgataatggc
    23821 gttttgtggc gcatctttcg catcggcgtc gtaaggcatc atacctacgc cgtagttaaa
    23881 tttggcgtac tcgcgaatgt tggcaagaga accggaagag gcggtggtca tcgcgcaatc
    23941 accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga agtcgccctt
    24001 cttgttcatc tcctcgagca tggcgatgtg tttcacctgc tccggcttgt tgaactccag
    24061 caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac cgttccaggc
    24121 gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc acttaatacc
    24181 ggaggctttc agttttgcgg aatagtccgc cagatcctgc caggttttcg gcggctgttc
    24241 cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg gagtcgagct
    24301 gttgaacggc tgggagagta agtggccggt tttgctgtcg gagtagtaac cggaaaccgt
    24361 cggcacaaac tgcgactcat cgaactgaat ccctgcctct ttaaacacgt catacaccgg
    24421 tttaatggct ttcgacgcca tcatggtggc ggtgccaact tcataaacct gcaaaatagc
    24481 cggagcgttg ccggtacgaa atgcggcaat ccccgcgctt aaattctgtt cgtagttgcc
    24541 tttataggtc ggtacaattt tgtaatccgg attttcggcg ttaaaacgtt gggccagaga
    24601 atccacctct ttacccagtt ccccttccat agaatgccag aacggaatgg tcgtcactgc
    24661 ctgtgcattc cccattaacg ccagtccgag cgccagtgct gaagctgtat aatgtaacgg
    24721 tttcatcgtt tatctctctt gttgtaccga atgcgcgaat tcacgcgttt tatgctcgcg
    24781 gggtaacatg acatgctcga attacagaaa aataactttt ttgttacatt tgtaagatag
    24841 taaggtgtca gaaagatgac aaggcggtga cggcgtgggt gagggaaaat gggagatggg
    24901 gcacggataa gcggaaaaat agaaggtctg aatcaaactc tacagattac tcatcgtttc
    24961 atgccggatg cggcgtaaac gccttatccg gcctacaaga tcgtgcaaat tcaatatatt
    25021 gcaactcacc cagtaggcct gataagcgca gcgcatcagg caattttgca tttgtcacct
    25081 gtctcaaagg agtcttttga ctccttatca atcaacgtgt tattacccgc ctaaatacga
    25141 acttctcact gcttcgttcg ccagcagcgc gtcgccggta tcggagagca ccacatggcc
    25201 gttttccagc acgtagccgc gatccgccag ctttagcgcc tggttggcgt tctgctcgac
    25261 gaggaagata gtcatcccct gctcgcgcag ctgctcgatg gtgtcgaaaa tttgctggat
    25321 aataatcggc gcaagaccga gcgatggctc atcaagtaac aacaaacgcg gattgctcat
    25381 cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg gacatggtgc ccgcccgctg
    25441 aacacggcgc tcatgcagac gtggaaacag ttcatacacc cactttatgc gctcctgaaa
    25501 ctggtcacgt tcggcaaaga agccgcccat cgccaggttc tcttccaccg tcatccgcga
    25561 gaagacgcga cgcccttccg ggacaatcgc taccgcttcg cgcatgattt tcgccgtctg
    25621 ccagtcggta atgtctttat catcaaacac aattcgcccg ctggtggcgc gcggatcgcc
    25681 gcataacgtg ccgagcaagg tggttttccc cgccccgttc gcgccaatga gagtgacaat
    25741 ctcgccctga ttgatatgca ggctcacctc atgcagcgcc tggattttgc cgtagtgggc
    25801 gctgactttg tcaaaggaca acatgacttt ttccatctta tgcctcacct aaataggcac
    25861 ggatcacgtc cgggttatta cggatttgct ccggcgtacc gtttgccagt ggcgtcccct
    25921 gattgaccac ataaattcgg tctgaaatcc ccatcaccag cttcatatcg tgttcaatca
    25981 acaagatagt ggtgttgtga tgattgcgca gttcggcaat cagctcatcc agctctttgg
    26041 tttcttttgg gttaagacct gccgccggtt cgtcgagcat taaaatctcc ggctgcgtca
    26101 ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata ggccaggtta cttgcctgac
    26161 ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt ggcggcgcgg tcgagcgctt
    26221 cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc agagaacagc ccggttttca
    26281 gttgctgatg ctgcgccacc agcaggtttt caattaccgt catttcacgg aacagacgca
    26341 catgctggaa ggtgcgcacc acgcccatgc gagcaatttg ctgccccggt aaaccttcaa
    26401 gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt gtagaaaccg gtcaggcagt
    26461 taaaaaccgt ggtttttccg gcaccgttag ggccgattaa cgagacgatt tcctgcgggt
    26521 acagttcaag attgacgttg ttcaccgcca gcaggccgcc gaagcgcatc atcagaccgt
    26581 taacagataa taatggctga ctcatgcctg ctctcctttc gctgcgccgt ttttcagctt
    26641 cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc cagatcatca tcagcaccat
    26701 caaaccaccg agcattaaca tgctgtattc gttgaaatca cgcatcaatt cgcgcgacac
    26761 caccagcaga acagccgcca gaatcactgc aaattgcgag cccataccgc cgagcaccac
    26821 tatcgccagc acaaaggccg attcagcaaa ggtgaaggat tccgggctga caaagccctg
    26881 acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg gcgctgatgg taaaagcggt
    26941 cagcttgata cggcgcgggc ttaagcccag cgaacgacag gcgatctcgt cttcacgcaa
    27001 cgcttcccac gcgcggccca gcggcatccg cagcaggcgg ttaatgacaa acaggcttaa
    27061 caccaccagc agcaacgcca ccaggtagag gaagataacg cgatcggacg gatcgtattt
    27121 caggccaaag aaattactga acgtgtccca gccgccttca cgagcggtac ggctgaactc
    27181 gagtccgaag agtgtcggtt tcgggatctg actgattccg ttcgggccgc cggtgatttc
    27241 agtgttattg agcagcaata tgcgcacaat ttcgccgaaa ccaagggtaa cgatcgccag
    27301 atagtcaccg cgcagacgca gcaccggaaa accgagcagg aaacccgccg ccgctgccat
    27361 cagtccggca atcggcaaac aggtccagaa ccccagaccg taatagtgat tgagcagcgc
    27421 aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc agcaccagca gaccagaaag
    27481 accaacaacc acattcagcc cgagaccgag gataatgtag atcatggtca gagtggcgat
    27541 atccaccgtc ccacgtgaca ccataaacgg ccacgccacc acaagcacca gcagcgccac
    27601 gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg ggcagaataa acttcggccc
    27661 ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa agctggaaga aaaagaccac
    27721 cgccgtgccg ataaacatcc actgccaacg gatgtctgaa gccgtgtcga ccaccagttt
    27781 ggtgccatcc agctccagtt gtacgcccat aaagacgccc gccaggacaa agaacatcgc
    27841 ggcagagagc agtgccattg caatatgcat cggtttcata ctttctctac ctccgggcga
    27901 cccagaatac cggtcggcat caccagcagc accagaatca gcagggcgaa tgagaccaca
    27961 tctttatatt ccgtactcag ataggcagaa gagagcgcct ccgcaatccc taggatcagc
    28021 ccgccaatca tcgcccctgg aatgctgcca atcccaccga gcaccgcagc ggtaaaggct
    28081 ttcatcccgg ccataaagcc gatatagggg ttaatgacgc agtagaactg accgagcagt
    28141 acgccagcca ctgccgccat cgccgcgcca ataacaaagg tcaaagcaat cacccggtcg
    28201 gtgttgatgc caagcaggct cgccattttc agatcttccg cgcaggcacg acaggcgcgc
    28261 cccatacggg aatagcgaat gaaaatcgtc agcgccagca tggcgaggaa ggtgacaatc
    28321 cagatcaccg cctgcatggt ggtaatagag gcagagaagt tttcgctatg gcccaccacc
    28381 cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg aaccttcggt caggctgacg
    28441 tagttttgca ggaagatgga cataccgatt gcggaaataa gcgcaatcag gcgcttagag
    28501 ttacgcaccg ggcggtaagc tacccgttcg atactccagc cgtaggcgct ggcgatgacg
    28561 attgcgccga cgaatcccgc cgccaccagc agccagccgg tatcaatgcc catcatcatc
    28621 agcgcggcga tgatcataaa tgagacgtag ctgccaatca tataaacctc gccgtgggcg
    28681 aagttgatca tgccgataat gccgtaaacc atggtgtagc cgatggctat cagcgcgtag
    28741 gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga aatacaaaaa ctgctcagac
    28801 ataaggtaac ctttctaaac ccgcccgcat tttacgggcg gtgggatgat cacttggctg
    28861 ccgtggatga accgtcggcg tgccactgga agacaccaaa atcaaatccc ttaagatcgc
    28921 ctttttcatc ccagttcagc ggcccaatca cggtgtttgc accgttagct tttaaatctt
    28981 tcaccagcgc cagcggctca tcgctgccgg tacgctcaag ggcagttgcc agagattgca
    29041 ctgccgcgta agtgatccag acataaggcc cggacggatc tttcttgtct gctttcagcg
    29101 catcaacgat gccctggttt gccggatcct ggtcatagcg ttttggcata gtgaccaaca
    29161 tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc gttaccgaca ccttctggcc
    29221 ccataaactg ggttttcagg ccaacggaac gggcttggcg cagcatctgc cccatttccg
    29281 ggtagtaacc gccgtagtaa acgaagtcga tattttcttt tttcaggcgg gcgatcagcg
    29341 cggagaaatc tttctccccg gcggtaatac cgtcgaagaa gacgacgttg gcgttagccg
    29401 ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc atactgttgt ttgtcgtgaa
    29461 tgatggcgat gcgctggggc ttcaccgtct caagaatgta ttttgccgcc gtaggcccct
    29521 gggaagagtc cagtccggca gtacgcataa tgtgttgata accgcgttgg gtcagctccg
    29581 ggttggtcgc tcccggcgag atcatcagaa taccttcgtc ttcgtagata tccgacgctg
    29641 gctgggtaga ggaagaacac agatgaccaa taacgtattt aatgccgtcg ttaacgattt
    29701 tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc gtcatattcc acgccaacca
    29761 gcttgtcgcc cttaattccc cctttgacat taatgtcttt aatcgcctga cgcgcgccgt
    29821 taaattccat atcgccccac tgggcaatcg ggccggacat cgcgccgaca acggcgactt
    29881 taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc aatcatccct gcgatgatag
    29941 ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg atgttgtgtt gctttgtttt
    30001 tatgtgttaa caaatcagac tgttcttttt ttatactgca ctgtttttgc ctgtctgatt
    30061 aaggggttag cgcagtattt tgtgatgata gcgattaaaa tccctatttt tcagtcgatt
    30121 aagaacagat aatattctga atttattgat agataaacag aaaaaagtgc ctttgtcagc
    30181 ataaaataac ggcacaaagg gcggaataat tcactatcat tcaggggatt atgctggaca
    30241 tttttcattc tctaatgttt taactttgta attattgctg ttaaaaaatt aatcacctgc
    30301 caaaagaaat aaaaaagaga aagcctccga ttaaattatt tcgctacact ggttccactt
    30361 ttgtgattta cacgggttac ccatgaagct gaccatcatt cgattagaaa actttagcga
    30421 ccaggaccgg attgacctgc aaaagatctg gccggagtat tccccttcct ctttacaggt
    30481 tgacgataac caccgtatct acgccgcgcg ttttaacgag cgtctgcttg cggccgtgcg
    30541 ggtaacctta agcggcaccg agggagcact ggattctcta cgcgtgcggg aagtcacccg
    30601 ccgtcgcggt gtggggcaat atctgctgga agaggttttg cgtaacaatc ctggcgtttc
    30661 atgctggtgg atggcggatg caggcgtgga agatcgcggt gtgatgacgg cgtttatgca
    30721 ggcactggga tttacggcac agcagggcgg ctgggagaag cgttaatcgt caagttgatt
    30781 tcaaaagtga tgaccaacga gaaattgcct gatgcgctac gcttatcagg cctataatgt
    30841 gtgctgcaat ttactgattt ctttggttct tgtaggccgg ataaggcgtt tacgccgcat
    30901 ccggcatgaa gcaacgtatt cgatattagc aatttggcgg caacccaaag ttgccgatta
    30961 acaattactt cgcatcggtc gccgtgccgt tggcgtgcca gtcaaatacg ccgaactcaa
    31021 agccctttag atcgcctttc tcatcccagg tcagtggccc catcacggta tccacggagt
    31081 tcgcttttag gtatttggcg atttcagccg gatcgtcaga ctgattcagg cccgcctgca
    31141 aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc gcttgggtcc tgttttttcg
    31201 ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg atcgtagttc ttcggcttgg
    31261 tcaccagcag cccttccgct gattcgcccg caatgttaga cagcgaaacg ttagccacac
    31321 cttccggccc cataaactga gttttcagcc ctgccgcgcg tgcctgacgc aggatttgcc
    31381 ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat attctctttt ttcagacgcg
    31441 ccaccagcgt tgagaaatct ttttccccgg cggtgatacc atcaaagaac accacgttag
    31501 cattgccttt cttcaggccg tcctgcaccg ctcgtgccag accttcgccg tattgctgtt
    31561 tgtcgtgaac gatagcaata cgctgcggtt tcactttctc aagaatatat ttcgccgccg
    31621 tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat cagctgatag ccacgggcgg
    31681 tcagctccgg cgcggttgcc gctggggtga tcattaaaat gccttcgtct tcgtagatgt
    31741 cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat cacatattta ataccgtcgt
    31801 taacgacttt gttcgccacc gcaaccgcct gtttcgggtc acaggcatcg tcatatttta
    31861 cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt gatatccgca accgcctgct
    31921 ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg accggacatt gcacccacga
    31981 ccgcgacttt aatatcttct gccagagcca tattgctgaa tgccagcgcg atacatcctg
    32041 ccagtaacgc tttacccttt atgttcatcc tgagaatccc cattcttctg gttattacgt
    32101 gtgttgtgat gttgttgttc agcactttat ttcgttttat gcatgactac ccgtgcttta
    32161 gcagcatact ctgctaaaac atacccgatt tttatgatat tggaatagct attttgacag
    32221 tttattaaca atctgcgtgg ggattggcgt tttgccggag ggggaattga ttattgtaga
    32281 ggcccaaaaa acaaaaaccc cggactctca tccagggttc tctgcttaat agcggaaatt
    32341 acgcttcaat agcggcgcga agttttttca tcgcgttctt ttccagttgg cgcacacgtt
    32401 cagcggaaac accgtaacgg tcagccagtt cctgcaacgt ggacttgttg tcttcgtcca
    32461 gccagcgcgc acggatgatg tcctggctgc gttcgtccag accatgcatc gcgtcggtca
    32521 gacggtttgc cgcctgctct tcccagttat catcttcgat gccgtcggca aagttagatg
    32581 atttatcctg cagatagagc accggtgcca tcggctggct gtcggaatcg tcgtcggaag
    32641 acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc catctcacgt acgtctttgc
    32701 tggttacgcc cagttcacgg gccaccattt cgacttcatc ctggttaaac cagcccagac
    32761 gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg cgctttggtg gtcgcaactt
    32821 tgacgatacg ccagttacgc agaacgtatt cgtggatctc tgctttgatc cagtggacgg
    32881 cgaaggagac caggcgcaca cccacttccg ggttaaaacg gcgcactgct ttcatcaggc
    32941 cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc atagcccgca taattacgag
    33001 caatatgaac aacaaaccgc aggtgagaca ggatcagcgt tttagctgct tccagatcgc
    33061 catggtaatg cagcttttca gccagcgccc gctcctcgtc agccgacaac atcggccacg
    33121 cgttagctgc ccggatgtag gaatccaggt tgccaactgg ggctaaagct aaactttgca
    33181 ttttgtcagt cattcaaatc ctctcaatcg atatcttctg gcgcttcagt ggtagcaaca
    33241 actgtgccaa agcttaagag caacgaggtt atcattcact gttttatcag accgtgattt
    33301 tatccacaag ttcaatgcaa gcttgtgaat aaattacgca caaaatgtga catagagatg
    33361 aaataccggg aagagacaac ggggtctctt tccctgctac ggaacccatt gcagggaaag
    33421 agtataacac gcttttatta ttcaggcgta aagtggcgta aatgttgtac cgtggcaagc
    33481 cacgctgcca cccagccaat catcgagcat accagcagca atagcaggca ttcatcgaac
    33541 gataaaccat tgatatcaaa cttcgttccg aaaacctgtg ccacttccgc caccgctgac
    33601 gacaaacgca gtaccagaat ttctgacaaa attaatgaca acaatgcgcc agaaaatccc
    33661 agcagcgcac caccgtacag gaacgggcga aggatgaaac catccgtcgc accaatcagt
    33721 ttctgcacgt tgatggagtc acggcgagca aagatgctca gacgtacgct gttgccgatg
    33781 acgaggaaca ctgccgccac catcaacacg ccgatcatcg ccgaaacgcg cccaaccagc
    33841 ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca tccgcacttc gtcaatgccg
    33901 ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt ccgtcccctg aaaatcgagt
    33961 ttcgggatca ccaccgccac tgccggaagc gggttttctt ccagcatatc cagcgcacca
    34021 ccaaaaccag accagttacg gaactcgccc agtgcgtctt cacgagaaag atagttcact
    34081 ttctccacgc cttgctcggc ctgcaactgt gccaccacgc ccgcagcggc gtcatcgtcc
    34141 agcgtttttt gcaagtaaac agtgatttgc ggtgacggat aatactgcgt cgccgcctga
    34201 ttaacgtttt tgtacaccat ataacagacg ctgggcagcg tcagggagat ggcgataacc
    34261 atcaccgtta aaaacgtggc gaacggtttg cttttcagat cctgcaatgc gccgtggaag
    34321 gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat ttaccggttt tggcgaggat
    34381 ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc cgccgaccga tttacggaag
    34441 cgatccagac gcccaccaaa ctgccgaata tgattgattg catcgcgctt attcatggcc
    34501 cacgcctcca tgcaagtgac catcgctcag ggtgagcatg cgataggaac gccgtgaaat
    34561 caggttaatg tcgtgcgttg ccatcaatac ggttacccca acgcggttaa attcttcaaa
    34621 cagacgtaga atcccttccg acaacgcgtc gtccaggtta ccggtcggtt cgtccgccag
    34681 cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca acacgctgtt gttcaccgcc
    34741 ggaaagctga atagggaagt tcttcgcttt gtccagtagc ccgactttat ccagtgccgc
    34801 cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg ataatcagcg ggatcgccac
    34861 gttatcgtag acagtacggt ccatcagtag atggtgatcc tggaaaatca tgccaatctg
    34921 gcggcgcaga aacggaactt cacggttttt cagacgcgtg atgtcatggc cgctaaacca
    34981 gattttcccg gcgctgggcc gctcaatccc acagatcagc ttcaggaggg tacttttccc
    35041 tgcgccggaa tgaccggtca gaaacgccat ctcacccggc tgcatatgga acgtaacgcc
    35101 ctgcagcgcc tgtctcccac cgagataagc cttgctgaca tgttcaaagc gaatcattgt
    35161 taatcctctc gggcaaaaag tgcctctata aagtcgtccg ccttaaacgg acgcaaatcc
    35221 tcaatacgtt cgccgacacc aatgtagcgg atagggatac caaactggtc agccaccgag
    35281 aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta gcgtgatgcc ggttaagcca
    35341 acagcttcgt ggaacagttt ggcctggctt accgcgttct gcccggtgct ggcatcaata
    35401 gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct tcatcacgcg gacgattttc
    35461 ttcaactctt ccatcagatg cgatttgttc tgcagacgtc cggcggtatc ggcaatcagg
    35521 acgtcgatat tacgcgcttt agctgcctga atggcgtcga agataacaga ggcggaatcc
    35581 gcaccggtat gctgggcaat caccggaata ttgttgcgct gaccccagac ctgaagctgt
    35641 tcaaccgcag ctgcacggaa agtatcgccc gccgccagca tcaccgattt accctgctgc
    35701 tcaaactgac gcgccagctt accaatcgtc gtggttttac ccacaccgtt gacgcccacc
    35761 atcaggatca cgaacggcgt tttatcttca acattcagcg gctcatcgac tttcgccaga
    35821 atctcaccca tctcttcttt cagcaggcca tagagcgcct cggcatcacg aagctgcttg
    35881 cgggatgcgc cttccgtcag attggtgata attttacgtg tggtttccac gcccacatcg
    35941 gcgatcaaca gctgttcttc cagctcttca aacagatcat cgtcgatttt tttaccgcgg
    36001 aacaggctga taaatccgga accgagattt tctttggttt ttaacaggct gcgtttcagg
    36061 cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct gggcaatttc ttcaaccggc
    36121 tgttcttctt ctgccggagg aactaccatc accgcctctt ctgccgcttc ggcagccagc
    36181 gcctgtgctt ccagctcttc gtcggtgatt tcttctttag ccgcttcttc ttctgccgct
    36241 tcgacaatct ctacggtttc cgcttcagcc tgccactctt ctggcgaaac ctcttcggcg
    36301 ttgacgtctt ccggcaacgg cagctcttca cgttcgatag ccactggctc cggcgtttct
    36361 tctacgaccg gttccggctg tgcaacaact tccgcttcag gctgcgcttt ttcactttca
    36421 acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag tttccgcctc ggcttcagta
    36481 tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct tcgcaggctc ttgcgcctga
    36541 acgatttctt ctacaaccgg ttgttcattc tgaacttctg tctctttttc cggggtctgc
    36601 tctttttgac caaagcccag ccaggaaaaa aagccacgtc ttttttcttt cgccatttgc
    36661 gactacactc ctcgctgttg cttcatggca cagcgttaac gctatgtaca tggcagctaa
    36721 aaaaatgatg aaatagtcta tcacttaact taattcacat caccgcctgc aagtatatgt
    36781 tatctggcgg attgagcaat ttatcatgaa aaaaccgaat cattccggca gcggccaaat
    36841 ccgcattatt ggcgggcagt ggcgaggccg taaactcccg gttcctgata gcccaggtct
    36901 gcgccccacc accgaccgcg tacgcgaaac gttgtttaac tggctggctc cggtcattgt
    36961 tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg ctggggctgg aagcgttatc
    37021 gcgctacgct gcgggggcaa cgttgattga gatggatcgc gtggtttctc agcagttaat
    37081 taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg gtgaacagca acgcaatgtc
    37141 attcctggcg caaaaaggca caccgcataa tatcgtgttt gtcgatccac cgttccgccg
    37201 tggcttgtta gaagagacga taaatttact ggaagataac ggctggctgg ctgacgaagc
    37261 cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc actgttccgg caaactggtc
    37321 attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg ctgtatcaac acgaagcaca
    37381 aggagaaagt gatgctgatt aatattggtc gtttgttaat gctctgcgtt tggggatttt
    37441 taatcctcaa tctggtgcat cccttcccgc gcccgctaaa tattttcgtt aacgtggcgc
    37501 tgatttttac cgtgctgatg catggtatgc agctggcgct attgaaatcc actttaccga
    37561 aagatggccc gcagatgacc accgccgaaa aggtgcggat tttccttttc ggcgtgtttg
    37621 aactgctggt ctggcagaag aaatttaaag ttaaaaaata accttattgt tcgccaacaa
    37681 agctaacaaa gcgcgttcct ttatagctca gcgtaccttt atcgcccacg gtcagggcgt
    37741 ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc tccgctttgc ggtttgaagc
    37801 ttgcctcata gcgcatactg gtgcctgccg gagtcacttc ctgctggcgc gaacggcgat
    37861 cgttaatcgg tttttcccgc ttgttgctca ccaccaccag cttttgctgg agcggagcca
    37921 tatcattatc agctttttcc cgtcgctgtt gcataaaacg aaacgatgcg gcgacgacaa
    37981 ttaagccaat gataacaata aagaaaagag gtggtttact catctttatc cctcatcgga
    38041 aaatgcggaa ttaagcatac cctgccagtt atggtgttgt catccgtcca ccctcgccac
    38101 taaactggaa gcaagaccgt aggcattccg cttacgaaaa aataacgaat tcaaggaact
    38161 aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat ggctatctgt ggatgcatcg
    38221 tatcgtgggc caacctggca acgctgggtg tttaaaccgt taacccttct tctcctgctg
    38281 ttactggcct ggcaagcacc gatgttcgac gccattagct atctggtgct ggcgggactg
    38341 tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac gtcaacgtct gatgtacgcc
    38401 atcggcgcgt ttttcctctc gcacctgctg tacaccatct atttcgccag tcagatgacg
    38461 ctctctttct tctggcctct gccactggtg ttgctggttc tgggtgcgct gctactggcg
    38521 attatctgga cgcggctgga agagtaccgt tggcctatct gcacgtttat cggcatgacg
    38581 ctggtgatgg tgtggctggc aggtgaactg tggttcttcc gtccgaccgc tccggcgctc
    38641 tctgcgtttg ttggcgcttc gttgctgttt atcagtaact ttgtctggct ggggagccac
    38701 tatcgccgac gcttccgtgc ggataacgcg attgctgcgg cctgctactt tgccggtcac
    38761 ttcctcatcg tccgctcact gtatctctga taaaacttga ctctggagtc gactccagag
    38821 tgtatccttc ggttaatgag aaaaaactta accggaggat gccatgtcga cacctgacaa
    38881 tcacggcaag aaagcccctc aatttgctgc gttcaaaccg ctaaccacgg tacagaacgc
    38941 caacgactgt tgctgcgacg gcgcatgctc cagctcgcca acgctctctg aaaacgtctc
    39001 cggcacccgc tatagctgga aagtcagcgg catggactgc gccgcctgtg cgcgcaaggt
    39061 agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg caggtgttgt tcgccaccga
    39121 aaaactggtg gttgatgccg acaatgacat ccgtgcccag gttgaatctg cggtgcaaaa
    39181 agcgggctat tccctgcgcg atgagcaggc cgccgacgag ccacaagcat cgcgcctgaa
    39241 agagaatctg ccgctgatta cgcttatcgt gatgatggca atcagctggg gtctggagca
    39301 gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc acgctagttg ggctgtaccc
    39361 gattgctcat caggcattac ggctgatcaa atccggcagc tacttcgcca ttgaaacctt
    39421 aatgagcgta gccgctattg gtgcactgtt tattggcgca acggctgaag ctgcgatggt
    39481 gttgctgctg tttttgattg gtgaacgact ggaaggctgg gccgccagcc gcgcgcgtca
    39541 gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc acgcgcctgc gtaacggtga
    39601 gcgggaagag gtggcgatta acagcctgcg gcctggcgat gtgattgaag tcgccgcagg
    39661 tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt gccagttttg atgaaagcgc
    39721 cctaaccgga gaatccattc cggtggagcg cgcgacgggc gataaagttc ctgcaggagc
    39781 caccagcgta gaccgtctgg taacgctgga agtgctgtca gaaccgggtg ccagcgccat
    39841 tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc cgcgccccca ttgagcggtt
    39901 tatcgaccgt ttcagccgta tttatacgcc agcgattatg gccgtcgccc tgctggtaac
    39961 gctagtgccg ccgctgctgt ttgccgccag ctggcaggag tggatttata aaggtctgac
    40021 gctgctgctg attggttgcc cgtgtgcgtt agttatctca acgcctgcgg cgataacctc
    40081 cgggctggca gcagcagcgc gtcgtggggc gttaattaaa ggcggtgcgg cgctggaaca
    40141 actaggtcgt gttacccaag tggcgtttga taaaaccggt acgctgaccg tcggtaaacc
    40201 gcgcgttacc gcgattcatc cggcaacggg tattagtgaa tctgaactgc tgacactggc
    40261 ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa gccatcgtac gcgaagcaca
    40321 ggttgctgaa ctcgccattc ccaccgccga atcacagcgg gcactggtcg ggtctggcat
    40381 tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct gccgggaaac atcccgctga
    40441 tgcatttgct ggtttgatta atgaactgga aagcgccggg caaacggttg tgctggtagt
    40501 acgtaatgat gacgtgctgg gtatcattgc attgcaggat accctgcgcg ccgacgctac
    40561 aactgccatc agtgaactga acgcgctggg ggttaaagga gtgatcctca ccggcgataa
    40621 tccacgcgca gcggcggcaa ttgccgggga gctggggctg gagtttaaag cgggcctatt
    40681 gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa catgcgcccc tggcgatggt
    40741 cggtgatggt attaacgacg cgccagcgat gaaagctgcc gccatcggga ttgctatggg
    40801 tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca ttaacccata accacctgcg
    40861 cggcctggtg caaatgattg aactggcacg cgccactcac gccaatatcc gccagaacat
    40921 caccattgcg ctgggactga aagggatctt cctcgtcacc acgcttttag ggatgaccgg
    40981 gttgtggctg gcagtgctgg cagatacggg tgcgacggtg ctggtgacgg cgaatgcgtt
    41041 aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca ttgagcgcga tcggtcccct
    41101 cgcccctctg gggagagggt tagggggtga ggaaaaggcg gcatcgaagc caatcagccc
    41161 ctatcaaccg cctttacgaa tcaaataacg ataaggcagt ccatccgtct ctttcgcaac
    41221 cagttcgtgt tccataaagg tacaaaaccc aggaatatcg cgggtagtgg ccggatcgtc
    41281 ggcgataatc agcaacgttt cgccaggctg catattgcgc acggttttgc gcaccatcat
    41341 caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg tggtcagggc tggaaaagag
    41401 atcggtcatt ttcttctcat cacttaaaaa aacggcgcta gtttacgccc tgtgagtccg
    41461 taagcaaacc aggttaacga ttgcgtgaaa attaaccatt gcattgtcaa cgtaaagcag
    41521 tatcatgcgg cggctcgaaa aaagggtaag cacgttatta tgttaaggta acagacgtgt
    41581 cgtacgtatt gggttccctc accccaatgg ttaatcaaaa aggtacaata tgaacgtttt
    41641 ctcgcaaact caacgctata aggcgttgtt ctggttatcg ttatttcatc ttctggtgat
    41701 cacctccagt aactatctgg ttcagcttcc cgtctccatt tttggtttcc ataccacctg
    41761 gggcgcgttt agctttccgt ttatttttct tgctaccgac ctgaccgtgc gtatttttgg
    41821 cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc cctgcgttat taatctccta
    41881 cgtcatctcg tcgctattct atatgggttc ctggcaggga ttcggcgcac tcgcccactt
    41941 caacctgttt gtcgcccgta tcgccaccgc cagtttcatg gcctacgcgc tggggcaaat
    42001 cctcgacgtg cacgttttta accgcctgcg tcagagtcgc cgctggtggc tggcaccgac
    42061 agcgtccaca ctgttcggta acgtcagcga cacgctggcc tttttcttca ttgccttctg
    42121 gcgtagcccg gatgccttta tggctgaaca ctggatggaa atcgcgctgg tcgattactg
    42181 tttcaaagtg ttaatcagta tcgttttctt cctgccaatg tatggcgtat tactcaatat
    42241 gctgttgaaa agactggcag ataaatccga aatcaacgct ttgcaggcga gttaaaggtt
    42301 cgtaatcaga gttgtgataa gatggatgaa tgagccgtta tggccgttta tcgaaaggaa
    42361 gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc ctgctggtta tggggcttgc
    42421 ggcctgtgat gataaagaca ctaacgctac ggcgcagggt tcggtcgcgg aaagtaacgc
    42481 taccgggaat cccgtcaacc tgcttgatgg caagttaagt ttctcgctgc cagcggatat
    42541 gaccgaccag agcggtaagc tgggaacgca ggccaataac atgcatgtct ggtccgacgc
    42601 caccgggcag aaagcagtca tcgtcatcat gggcgatgat ccgaaagaag atctggcggt
    42661 gttggcgaag cgtctggaag atcagcaacg tagccgcgat ccgcagctgc aagtggtaac
    42721 caataaagcc attgagctga aaggtcacaa aatgcagcag ttagacagta ttatctccgc
    42781 gaaaggccag acggcgtact cttccgttat tctgggtaac gtgggtaatc aactgctgac
    42841 catgcaaatt acgctgccag ctgacgatca gcaaaaagcg caaaccaccg cagaaaacat
    42901 cattaatacg ctggttattc agtaagtttt aagatgatga ggcggcctca gggacgtgtt
    42961 ccggaggccg tttttttaat cgccacgtca gtaataacgc gattgcgacc agtcccgccg
    43021 ccgccagata aatcaccggt acgcccgccc agctcatcac cagcccagcc agtggtccag
    43081 tcacgccaag cgataaatcc ataaatacgg tgtaagttgc cagcgccgcc ccctaatttt
    43141 gctgcggaac cgcttttacc gccactacac ccaatgccgg gaacaccagc gaaaacccgg
    43201 cccccgccag taagacgccg attttcgcca tccacggcat agtcgccacg ccaaccagta
    43261 gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac gtttaagcca ccgatacggt
    43321 taatgccgtt agggaataac aaacgcgtac cgacaaacgc acagctaaac agcgtcagcg
    43381 cgaaagccgc accgtcccaa cctttagcgt cataaaacag cgtgataaag gtggcgatga
    43441 cgccaaatcc ggcggaagcc agtgccagcg ccataccgta cagccagacg cgcccaagca
    43501 ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac cgtcggacgc gggatcgcca
    43561 acaaaatggc caccagcgcc acgcccataa tgattaacgc taacgcctgc aagccgcccc
    43621 agtgataaaa cacgacgcct aacggcgcac ccatcgccat cgccccgtaa gtgacaatgc
    43681 cgttccacga aatcacccgc ccgatatgca gcgagccaac cactccaacg ccccataggg
    43741 tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat gacgcgcccc aggcaaagta
    43801 ataacaggct gatgacaggc agactggcgg ttaatcctgc cgtcagatac cccagaccgc
    43861 tcaaaaagca gccgcataaa ccgaagacga caatcttttt gggtcccagc aaatcggcgt
    43921 aacgtccggc atgagggcgg ctcagcaagg tggcgaaata ttgcaggctg ataaccaatc
    43981 ctgcccagaa ggcgctaaag cccatcacat catggacata gcccggtaat acagcgagcg
    44041 gcaacccgat ggtgaggtag ctggcgaagt taaacatgac aatggagaca atgcgcaaat
    44101 tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc gggcatgagg atcaccacat
    44161 ttttacaaca gtgtttcatt tttaccatgt gctgacgtga aaatcagcag taagaatcag
    44221 aatattgctg gcgtgactcc cgctacactt aacacaaaaa gtcacaagga agccccaatg
    44281 gaaacccctc aacccgataa aacgggcatg cacattctgc tcaagttggc ctcgctggta
    44341 gtgatcctcg cgggcattca cgcagcggca gatatcattg tgcagctgtt actggcgctg
    44401 ttttttgcca tcgtcctcaa cccgctcgtt acctggttta ttcgtcgggg agtacaacgc
    44461 cccgttgcca ttacgattgt agtggtggtg atgctgatcg cactaaccgc gctggtcggc
    44521 gtactggcgg catcgtttaa cgaatttatc tctatgctgc cgaagtttaa taaggagctg
    44581 acgcgcaaac tttttaaatt gcaggagatg ttgccttttc ttaatttgca tatgtcgccg
    44641 gagcgaatgc tgcagcggat ggactcggaa aaagtggtta ccttcaccac agcgctaatg
    44701 accgggcttt ccggggcaat ggcgagcgtg cttttgctgg tgatgaccgt agtttttatg
    44761 ctgtttgaag tgcgccacgt cccttacaaa atgcgttttg cgctgaataa cccacagatt
    44821 cacatcgcag gattacaccg cgcacttaaa ggcgtttcgc attatctggc gttaaagacg
    44881 ctactcagtt tatggacagg tgtcatcgtc tggctggggc tggcgctaat gggcgtacag
    44941 tttgcgctga tgtgggcagt actggcgttt ttgctcaact acgtgcccaa tatcggcgcg
    45001 gtaatttccg ccgtaccgcc aatgattcag gtgctgctgt ttaatggtgt ttacgaatgt
    45061 attctggtcg gcgcattgtt tttagtggtc catatggtca tcggcaatat tttagaacca
    45121 cggatgatgg gccatcgcct ggggatgtcc accatggtgg tatttctttc attgttaatc
    45181 tggggatggc tgctcggccc ggtagggatg ctactttcgg tgccattaac cagcgtgtgt
    45241 aaaatctgga tggaaacgac gaaaggtggc agcaaactgg cgattttact ggggccggac
    45301 agaccgaaaa gtcggttacc gggatgaggc gacaagtgat acgatacgca ctttcatttt
    45361 ccattaaacg ttggccctga tatgtatcgg atagttctgg ggaaagtttc gaccttaagc
    45421 gcagctccac tgccaccggg tttacgcgag caagcaccgc aaggtccacg acgcgaacgc
    45481 tggctggcgg ggcgtgcatt gctttcgcac acgctttccc cgctaccgga gatcatctat
    45541 ggcgaacaag gcaaacctgc attcgcgccg gaaacgccgc tatggttcaa cttaagccat
    45601 agcggtgacg atatcgctct gctgttgagc gatgaaggcg aagtcggctg cgatatcgaa
    45661 gtgattcgcc cgcgcgccaa ctggcgctgg ctggcgaacg ccgtgttcag cctcggggaa
    45721 cacgctgaaa tggacgccgt gcatcctgat cagcaactgg aaatgttctg gcgcatctgg
    45781 acgcgcaaag aagccatcgt taaacagcgt ggcggcagcg cctggcaaat cgtcagcgta
    45841 gatagcacct atcactcctc gctgtcagtc agccattgtc agctagaaaa tttaagcctt
    45901 gcgatctgca cccccacccc ctttacgctc accgccgaca gtgtgcaatg gatcgattca
    45961 gttaactgat ccgcccaccc gactgcccat ctattgatcc agaacaggta atcagtatga
    46021 cgaatactta aaatcgtcat acttatttcc gccatctatt ttaatccatt ggggttacca
    46081 tgctctccac actccgccgc actctatttg cgctgctggc ttgtgcgtct tttatcgtcc
    46141 atgccgctgc accagatgaa atcaccaccg cctggccggt gaatgtcggg ccactaaacc
    46201 cgcaccttta cacgcctaac cagatgttcg cccagagcat ggtttatgaa ccattggtga
    46261 aatatcaggc agacggttcg gtgatcccgt ggctggcaaa aagctggact cattcagaag
    46321 atggtaaaac ctggaccttc accctgcgtg atgacgtgaa attctccaac ggtgaaccgt
    46381 tcgatgccga ggcggcggca gaaaacttcc gcgcagtgct cgataaccgt caacgtcacg
    46441 cctggctgga gctggcaaac cagattgttg atgttaaagc actcaataaa acagagctgc
    46501 aaattaccct gaaaagcgcc tactatcctt tcctgcaaga actggccctg ccccgtcctt
    46561 tccgttttat cgctccctcg cagtttaaaa accatgaaac catgaacgga attaaagcgc
    46621 cgattggcac cggaccgtgg attttgcagg aatcgaaact gaatcagtac gatgtcttcg
    46681 tccgtaacga aaactactgg ggcgaaaagc cagcgattaa aaagatcacc tttaacgtca
    46741 tcccggaccc gactacccgc gcggtggcgt ttgaaactgg cgatatcgac ctgctgtacg
    46801 gaaacgaagg gttattaccg ctcgatacct tcgcccgctt tagccagaat ccggcttacc
    46861 acacccaact gtcacagccg ctcgaaaccg tgatgctggc gctcaatacc gccaaagccc
    46921 ccaccaacga gctggcagta cgtgaagctc ttaattacgc ggtaaacaaa aaatcgctga
    46981 ttgataacgc gttatatggc acccagcagg tcgccgacac cctgtttgcc ccttctgtgc
    47041 cttacgctaa tctcggcctg aaaccgcgcc agtacgatcc gcagaaagcg aaagagttgc
    47101 tggaaaaagc cggttggacg ctgcctgcgg gcaaagacat ccgcgagaaa aatggtcagc
    47161 cgctgcgcat tgaactgtcg ttcatcggca ccgatgcgtt aagcaaatcg atggcggaaa
    47221 tcattcaggc tgatatgcgc cagattggcg cagatgtctc gctgattggc gaagaagaga
    47281 gcagtatcta tgctcgtcag cgcgacggtc gttttggcat gattttccac cgcacctggg
    47341 gcgcgccata cgatccacac gccttcctga gttcaatgcg cgtaccgtcg cacgctgact
    47401 tccaggcaca gcaaggatta gccgacaagc cgctgattga taaagagatc ggcgaagtgc
    47461 tggcgaccca tgacgaaacg caacgtcagg cgctgtatcg cgacattctg acccgtctgc
    47521 atgacgaggc ggtttatctg cctatcagct acatctcgat gatggtggta tcaaaaccgg
    47581 agctgggtaa tatcccctac gcgccgatcg ccaccgaaat tccgttcgaa cagattaaac
    47641 cggtgaaacc ttaatgttgc gttacgtatt acgccgcttt ctgctgctga tcccgatggt
    47701 gctcgccgcc tcggtgatca tttttctgat gctgcgcctc ggtaccggcg acccggcgct
    47761 cgattatctg cgtttatcta acctgccgcc gacgccggag atgctggcct ctacccgcac
    47821 catgctcggg ctggatcagc cgctgtacgt ccagtacggt acctggttgt ggaaggcgct
    47881 gcatcttgac tttggtatct cattcgccag ccaacgcccg gtactggacg atatgctgaa
    47941 cttcctgccc gccacgctgg aacttgcagg tgcggcgctg gtattaattc tgctcacttc
    48001 cgtaccgctc ggtatctggg cggcgcgcca ccgcgatcgt ttgccggatt tcgccgtgcg
    48061 ctttatcgcg tttcttggcg tgtcgatgcc caacttctgg ctggcatttt tgctagtgat
    48121 ggcgttttcg gtgtatctgc aatggctacc cgcgatgggc tacggcagct ggcagcacat
    48181 cattttacct gcggtttcca tcgcctttat gtcgctggcg attaacgcgc gactgctgcg
    48241 cgccagtatg ctggacgtcg ccggtcagcg tcacgtcacc tgggcgcggc tacgcggcct
    48301 gaatgacaaa cagaccgaac gccgccatat tctgcgcaat gcctcgctgc cgatgatcac
    48361 cgccgtgggg atgcacattg gcgaattgat tggcgggacg atgattatcg aaaacatctt
    48421 tgcctggcct ggcgtcgggc gctatgcggt gtcggcgatt tttaaccgtg actatccggt
    48481 gattcagtgc tttacgctga tgatggtggt ggtttttgtg gtctgtaatt tgattgtcga
    48541 tttgctcaac gccgcgctgg acccgcgcat tcgtcgtcat gaaggagcgc acgcgtgaac
    48601 tttttcctct cttcccgctg gtcggtacgc ctggcgctga tcattatcgc cctgctggcg
    48661 ctgattgcgc tcaccagcca gtggtggctg ccgtacgatc cacaggcaat tgatttgccg
    48721 tcgtgcctgc tttcgccgga tgcgcagcac tggctgggca ccgatcactt aggtcgcgat
    48781 attttctcgc ggctgatggc agcgacccgc gtgtcgctcg gttcggtaat ggcctgcctg
    48841 ctgctggtgc tgatattagg gctggttatt ggcggcagcg ccgggttgat tggcgggcgc
    48901 gttgatcagg ccaccatgcg cgtcgccgat atgtttatga ccttcccgac ctcgattctg
    48961 tcgttcttta tggttggcgt gctcggcacc gggctgacca acgtaattat cgccatcgcc
    49021 ctgtcgcact gggcgtggta tgcacgcatg gtgcgcagct tggtgatttc actacgccaa
    49081 cgcgagtttg tgctggcgtc acggctttcc ggtgcgggcc atgtgcgggt gtttgtcgat
    49141 catctggcag gcgcggtgat cccttcgctg ctggtgctgg caacgctgga tatcggccat
    49201 atgatgctgc acgtcgcggg gatgtctttc cttggcctcg gtgtgaccgc gccgaccgcc
    49261 gaatggggcg tgatgattaa cgacgcgcgc cagtatatct ggacccagcc gctgcaaatg
    49321 ttctggccgg ggctggcgct gtttatcagc gtgatggcct ttaacctggt gggtgacgca
    49381 ctgcgcgatc atctggatcc tcatctggtg acggagcacg cacactaatg ccgcaacaga
    49441 ttgaactacg taatatcgcg ctacaggccg cgcagccgct ggtacacggt gtatcgttaa
    49501 ccctgcaacg cgggcgcgtg ctggcgttag tcggcggtag cggcagcggg aaatcattaa
    49561 cctgcgccgc gacgctgggc attttgcccg ctggcgttcg ccagacggcg ggggaaattt
    49621 tagccgatgg caaaccggtt tcgccttacg ccctgcgcgg catcaaaatt gccaccatca
    49681 tgcagaaccc gcgcagcgcc tttaatccac tgcacaccat gcacacccac gcgcgggaaa
    49741 cctgcctggc gttagggaaa cccgccgatg acgctacgct taccgctgcc atagaagcgg
    49801 tggggctgga aaacgccgcg cgcgtgctga agctgtaccc gttcgagatg agcggcggca
    49861 tgttgcagcg catgatgatt gcgatggcgg tgctgtgtga atcaccgttt atcatcgccg
    49921 atgaaccgac caccgacctc gacgtggtag cacaggcgcg catcctcgat ctgctggaaa
    49981 gcattatgca aaaacaagcg ccgggaatgc tgctggtcac ccatgatatg ggcgttgtgg
    50041 cgcgtctggc ggatgacgtg gcggtgatgt cacaaggtaa aattgtcgaa cagggcgatg
    50101 tagaaacgct gtttaacgcc cccaaacata cggtgacgcg cagcctggtt tccgctcatc
    50161 tcgccctcta cggtatggag ctggcatcat gaccttactt aacgtctgcg gcctttccca
    50221 tcactatgcc cacggtggat ttagcggaaa acatcaacat caggcggtgc tgaataacgt
    50281 ttccctgacc ctgaaaagcg gcgaaaccgt cgccctgctg ggacgcagcg gctgcggaaa
    50341 aagcaccctc gcgcggctgc tggtaggctt agagtctccc tcacaaggga atattagctg
    50401 gcgcggcgaa tcgctggcga aacttaatcg cgcccaacgt aaagccttcc gccgtgatat
    50461 tcagatggtg tttcaggact ccatcagtgc cgtgaatccg cgcaaaaccg tgcgcgagat
    50521 cctgcgtgaa ccgatgcgcc acctgctatc actgaaaaaa tccgaacaac tggcgcgcgc
    50581 cagcgaaatg ctgaaggcgg tcgatctcga tgacagcgtt ctcgacaaac gcccaccgca
    50641 gttaagcggc ggccagctcc agctcgtctg cctggcacgc gcgctggcgg tcgaaccgaa
    50701 attgctgatt ctggatgaag ccgtttctaa ccttgatctc gtgttacagg cgggcgtcat
    50761 tcgcctgctg aaaaatctac aacaacagtt tggcactgcc tgcctgttca tcactcatga
    50821 cttacgcctg gtggaacgct tttgccagcg agtgatggtg atggacaacg gacaaatcgc
    50881 cgaaacccag gcggtgggag ataaattaac cttttcctct gacgccggac gtgtgctaca
    50941 aaacgcggta ttacccgcat tccccgtgcg ccgtcgcacc tcagaaaagg tttaacgcca
    51001 atgcaacgag tcaccatcac gcttgatgac gatttactgg agacgctgga cagcctgagc
    51061 cagcgtcgtg gttataacaa ccgttccgaa gctatccgcg atattctgcg tagtgccctg
    51121 gcgcaggaag ctacccagca gcacggcaca caaggtttcg cggtgctgtc gtatgtgtat
    51181 gaacacgaaa aacgcgactt agccagccgt attgtttcca cccagcatca tcaccacgac
    51241 ctctccgtcg ccacgctaca tgtgcacatc aaccacgacg actgtctgga aatcgccgtg
    51301 ttgaaaggtg acatgggtga cgtgcagcat tttgccgatg acgttatcgc ccagcgcggc
    51361 gtgcggcacg ggcatttgca gtgcctgccg aaggaagatt gagttatttc taaaacaagg
    51421 aaagaaaaga tattaactga acttaattta taaaattaag ttcagttgca tagatagatc
    51481 ctgtaaaggc agatattctg cgtaatgtac gtcgctaaaa tttataaatg attcaaacgc
    51541 tcattcagtg tttcaataat ataggtatta acagagactt gttgctgcgc agccgcatta
    51601 ttaaggcgtt cactcagcga ttctggatag cgtaacgtaa aggtcttaat cttttctgta
    51661 cgcgcatacg gctcaatacc cgcagcttta caatcctcaa gatactctcg cagggaaagc
    51721 tctccttctt tttgcagccc ctgaatactg tcagagacaa aatcacaata acctgacaag
    51781 cccaggaatt tgccacgaaa tgcattcagt tctggcacgt aggtaataac agctggttgc
    51841 ccggcaatct ccatagagtt tggcgtcttc agtttgatca tggttttacc cctatactta
    51901 gtaaccagtc acgcacgctt tctacagcgc ctttatcggt atctggcgac ggatgaggcc
    51961 gatggaaaca cgcaacgctc atatttagta tgaacttgca acgcgaacct cttccttcct
    52021 taatttctcc gcctaatgct ttaaccagtg actctatatc agaccacttg atcccttgag
    52081 gaacaggtgt tttaaatatt tgatccaatg tatttttttg tttcttccgt aaagcctgta
    52141 ccttggttct catggcatgt ccttatgact tcactttatg acatcacagt atgaagtcaa
    52201 gtttcccttc cttacgccat tgtcccaatc gtcttcctga atcgcagcag tgcgatggtg
    52261 aaaaacgcgc cgccaattgc catcagcgtc aggaactgcg gccagacgat ttcgaatccg
    52321 gcaccccggt agaggatggc ctgcgcgagg ctaacaaagt gtgtcgtcgg catggtcagc
    52381 ataatgtcct gcaccatctg cggcatactt tcgcgcggcg tggatccacc ggaaagcatt
    52441 tgcagcggca gcagcaccag aatcaccagc agccccagtt gcggcattga acgcgctatc
    52501 gtccccataa aaatgccgat tgacgtggtg gcaaacagac tgagcgccac gcccagcata
    52561 aacagcggga tcgagccttc aatcggtacg cccagtacac ctttcaccat cagcaccagc
    52621 gataatcccg ataccaccag caccaccagc cccatcgacc agatcttcgc catcatgatc
    52681 tcaaacggcg ttatcggcat caccagtaaa tgttccaccg tgccgtgttc acgctcgcgg
    52741 atcagcgccg atccggtcaa tacaatcgcc agcatggtaa tgttgttgat gatcgccatc
    52801 accccgccaa accacgcggg atcaaggttc gggttaaagc gcatccgggt ttccagcgat
    52861 accaacggtt cgctgttatc acggtagcgc gcgacaaagc tgttcacttc accgttgata
    52921 atattctgga tatacccatt gccggtaaat gcctggctca tgcgcgtggc atcgacgttc
    52981 acctgaatat ccggctggcg tccggcgagg acatcacgct gaaaattagg cggaatattt
    53041 atcgcgaagg tatagcgtcc ggcgtccagt ccggcatcca tctcatcggc ggtgatcatc
    53101 tccggtggca aaaaccacgg acgatagaag ctgttaacga tccggttcga taactgcgat
    53161 tgatccatat cggcaatggc gatcggcgcg aggttcaacg atcctggcgt aacggtcgct
    53221 gacgaataca ccgacaccgt aaacgagaag acaatcagcg tcagcatcgc tttatcaccg
    53281 agcagactgc gcaactcttt gatacccaga ttaaaaatat tgcgtaactg gcgcatcatc
    53341 cctcctgttt tttcagcagc aggatgctta agcccatcac cagcgggatg gctatcagca
    53401 acgggataaa aagttgccac aaatcagtca gatccagcgc tttcgagaac gttccgcggg
    53461 cgatagtcag aaaatgactg gtcgggtaaa cctcgccgat ccaacgtcca ggcccttcca
    53521 gcgaagctac cggatcgatc atcccggaaa actgtgtcgc cgggatcaat gtgataatcg
    53581 ccgttccgaa aatggcggca atctggcttt tcataaaggt ggagatcagc agccccattc
    53641 cagtggcaat gatgatatac agcagcgccg ccaaggtgag cgtcaggaaa ctgcctttat
    53701 gcggtacgcc aaacacaaac accgacaggc cgcagagcag gaaaaagttc agcatcccca
    53761 gcgcgatgta cggcagctgt ttgccaagca aaaattcgct acgggtggtg ggggtcacgt
    53821 aaaggttgat aatcgaccct agctcttttt cccgcaccac gctaagggcg cttagcattg
    53881 acgggatcat catcagcaga agcgggatca ccgccggaac aatcgctggc aggcttttta
    53941 cgtccgggtt atagcgataa cgcgtctcaa tgttcatcag cccgctttgg ctggcgggtg
    54001 tcgattgtcg gctcgccaca tcctgaagcc aactctggtg cattgcctgc acgtaacctt
    54061 ttaccgtttc ggcacgactg ggcatcgctc cgtcgatcca gacgccgagt tccacaggcg
    54121 taccacgcgc gatatcgcgc ccgaaattgg gcgggatctc aatggccacc gtgatatcgc
    54181 ccgcacgcat ccgacgatca agctcgtcat aactggtgag cggcggctgt tcgataaagt
    54241 aacgggaacc ggagaggttg agtgtccacg cctggctact gacggtctgg tcgcggtcga
    54301 gcaccgcaaa gcgcaggttt tccacatcca tactgatgcc gtaacccatt atcagcatca
    54361 ggatcaccgt tcccatcagc gccagcgtcg aacgtactgg atcgcgtcgc agttccagcg
    54421 cttcgcggcg gctgtagcta aacagacggc gcaggctaaa tccctgacgc ggcgcgtggg
    54481 tggtgtcgtg taccacgggc ggcgcttcgg cttcgttgct ctgccctgcc gcttcctgca
    54541 aataggcgat aaatgcctct tccagactgg cggctccgcg tttctcaacc agttcctgcg
    54601 gtgtaccgct ggcaagcact tttccggcgt gcatcagtga gatgcggtcg caacgttccg
    54661 cttcgttcat aaagtgggtg gagatgaaga tagtcacttt gtcctggcgc gagagatcga
    54721 ccatcaactg ccagaacata tccctcgcca ccggatcgac accagaagta ggctcatcga
    54781 ggattaacat ctccgggcga tgaatcaccg ccaccgccag cgaaagccgc tggcgaatgc
    54841 cgagcggcaa tgactccggc agaacatctt caacgtcgtt gagcttaaaa cgctcgctca
    54901 tttcagccac tcgcgcggga atttccgctt ccgggatgtg aaacaaacgg gcatgtaact
    54961 ccaggttttg ccgcacggtg agttcgttat agagcgaaaa cgcctgcgac atatagccca
    55021 cacgacggcg ggtatcgata tcttttggat caaccggttg cccaaatagc cacgcctcac
    55081 cttcgctggc gggcagcagt ccggtaagca ttttcatggt ggtggattta ccgcagccgt
    55141 tcgaaccaag gaatccaaaa atctccccgc gtggaatgcg gaaattgacg tgatcaacgg
    55201 caacgaagga accaaaacgc atggtcagat cgcgcgcttc gatggcaatc tctgcgtttt
    55261 caggttgata cggtgggatc actaccgcct gatgcgcctg gcgttgcgct tgcggtaaca
    55321 gattaataaa tgcttcttcc agcgtggcgc tttgcgtttg ctgccgcagc tcttcggcgc
    55381 tgccggttgc cagcacttct ccggcattca tcgctaccag ccagtcgaag cgttcggcct
    55441 cttccatata ggcggtggcg accagcacgc tcatattgct ctgccgctgg cgaatactgt
    55501 cgatcagatc ccagaactgg gcgcgggaga gcgggtcaac ccccgttgtt ggctcatcaa
    55561 ggatcaacag ttccgggtcg tggattaacg cgcagcacag cccaagtttt tgcttcatcc
    55621 cgccggagag tttacctgcc ggacgatcgc gaaacggtgc taacccggtg ctggtcagca
    55681 gctcattaat tcgcacttcc cgctccgctt tatcgtgacc gaacaggcga gcgaaaaaat
    55741 cgacattttc atacaccgac aaggtgtggt agaggttttt gcccagcccc tgcggcatcc
    55801 aggcgatgcg cgggcagacg tctcggcgat gcttcgggtc gtgcatatcg ccacccagca
    55861 ccaccacatt gccctgctca atgacgcggg caccggaaat cagtgacaat aaactcgact
    55921 tcccgacgcc atccggacca atcagcccga ccatgcaacg ggccggaata tcgagagtga
    55981 tattgttcag cgcaacggtt tttccataat gctggctcac gcccgccagt tgcgcgacag
    56041 gcgggacagg aaccagttcc agatgcgtca ttgcggcaac ctcaccacga ggtcgtcagg
    56101 ccacggaagt tcttcattca cccgcaccca cgctacgccc ggtaaaccgg ttttgacata
    56161 ttccagatgc tgctggagta attccggtgg gatacgcgct ttgacgcgga acatcagttt
    56221 cagccgttca tcgctggttt cgacggtttt tggcgtgaac tgggcgacac tggcgacaaa
    56281 actgatggtt gcaggaatac gcagatctgg cgcggcatcg aggatcagcc gggcttcacc
    56341 gcccagtttc agcgtgcccg cctgttcggt tggcaggaag aaggtcatat agacgtcgct
    56401 gagatcgacc atattcagca cccgaccgcc tgccgccagc acttcccctg gctcggcaac
    56461 ccgatactgc acgcgtccgt cacgcggggc tttcagttca ctgtcatcga tatctgcggc
    56521 aatgcgccgt tcagtggctt gcgctgcttc gacccgagtt tgcgcctgaa tgatattggt
    56581 gcgtgctgct tctatagccg ctttagaagc cgaaacctgg gctttcgctg attccagcgc
    56641 agctcgggcg ctctcagcgg cggcgcggtc gtcatccagc tgttgcgcag aaatagcccc
    56701 tcgttgggcg agcgagcggg aacgcgtatg acgtttggca acagagtcca gttcggcctg
    56761 gcgttgattg accagcgact gtgcggcacg agtttcactt tgtcgttgct ccagcaaagc
    56821 ctgcgcggcg gcaacggcgc tttgtgcctc tttgatttgc gcgatggctt ccagtcgctg
    56881 ttcctgcaac acgcgagtat ccatcttcgc cagcacttcg ccttcgcgaa caaactggcc
    56941 ttctttcacc agaatggtgt cgatacgccc ggcagttttg ctggcgatat ccacttccgt
    57001 cgcttcaatg cgcccattgc tgacagcaaa gccttccggc acacccgccg ggcgcaacag
    57061 ccaccacgcc acgacggcag ccaccgccag tgccccgaca acccaccacg ccagatggcg
    57121 cttactctta tccataatcg acccgccata atccctgtaa gcaaacgacg cgtgttacaa
    57181 ctgccgccga acgacgttca gcggattctt ccatccggga taagaaggaa acgacaaata
    57241 cgaccagcgc gcagagcatt gagcgccaac ggggaaggct tagcgaaaac gggtagtaat
    57301 gttgtcatca tcagcattcc tggccgtaaa tgaaacacgc atactcataa gtgtgatcta
    57361 taaaactggc tgacaaaacg gtatccatcc tgttggatcg cgtttcacgc ctgttcagtt
    57421 gcaggaacag agcattcctt tttacgtttt atcaacagtc tcaaccagat agtaacatta
    57481 atattcatgt tgtctatggt tcagagctgt aacattgtgc gccttctaat caaaggcata
    57541 agtccatttc tgtttttttc atgctggtaa gacttccagg gagtggttat ctctggccct
    57601 gcaccagggt taatttggca attatgcatg ctgaacattc ttccagcatg tttactgtca
    57661 cgggcgttaa gacctaaaac tgtccccgtt tcacctatgc gttctctccc accatttccg
    57721 ttatgtgcaa caacttgatt tatacctaac atttgtgaac atgctaatag tgcttcaagt
    57781 ttaggtctgt aatctgtcga aacagaaaaa taacgattaa aggatcgaca agagatagcc
    57841 cggctagttt taaacttttt gccattaatt atagcatgat gctttttatt aataatttcc
    57901 gctaattcga ccggatcgag agcattggtc atttcatcaa tagagacagt tatcggtcct
    57961 ataatgtaac gttttcgctg atcatcaaaa gttatcccat gatgatttgc cattattttg
    58021 tatttggggt catagaaaca tacaggataa tcttttatcc cgtcgtaggt atcctcatca
    58081 agtggtttca tgcgcgtgaa gttttgcata tagttaccat tgcaattcgt ttcatgattt
    58141 ccagctaata catgaacatt tttattgata ctcattatgc gttttaacaa atcgatgata
    58201 aattgaacgc cacagatatt actaaatcga tcgccggttt ggtcgccaag aaacgtacat
    58261 ggcgtgactg cgtctttatt aataaccaaa taaggctgaa gactttctaa aacattatct
    58321 cttctgttca gtatactgat tattgtatta acattagtgt caagttgctc attaaagttc
    58381 tcccttagca atacgttgag aacttcatct tctatattaa gcaactggct aaggaaattt
    58441 tctccttcag ccatgattcc caaatgtcct gttgcaaaaa gcgcgtacaa aactgcaccg
    58501 actgagctat cggtatcgcc aaaataggtc ggcctggtac ttgtgggatc aacaggtgaa
    58561 caattggaga aataggttct gtcagcgaac tggggtttta taattgtttc gaatttaata
    58621 tcaaatacat tgtattgcga tatttcccag ttccagatgg gtttgtacga ctcagtaact
    58681 gcaacatcat tagcattatc atttactgtc atattattaa gtaaataaac aatgctgcac
    58741 caattaatat cggtattatc aagagggcaa ccgtgatttt tactattaat tgtcacaata
    58801 aactgataac agttattcct acgtagaata tgagcgcgca tgcccgatag catactatat
    58861 ttttctgaat gattcgtttg tatggtaaat gaaattttgt catcttgaat agattgttgg
    58921 aaacgttcta tttgccaatc atcaattttt tccacaactt caccgagcat tgtcctggca
    58981 atacttgata tatcatcacc tataccattt gcaatatacc caaacagttc tttagtgaag
    59041 ttggtaacat gctcttgcac catatcattt gttgcgattg tcgttgtact gccattagtg
    59101 cctgccacag ttccaatttt catcttttac ctcatattat tcaccgcgag gaatacatag
    59161 ttaacatttt atcttatgac atacacctca tattcgatgg gaaaataatt atattttgca
    59221 caaatcattc agccttaaag cataatatga aatttcttat ctcatagcag agaaagtccc
    59281 taaagattgt agaggcgtca tcagtacata cctctacaat cgattaagaa aacctatgaa
    59341 atactggcaa aattaaaaag agataccaca gcactcatga agttttattg aggttgaaaa
    59401 caactaagcc ctgggacacc ttttttatca ccacgttcgt taggtgctat cttccaagaa
    59461 tttagcccca tggtataatt atcaattttt tgatgctggt ctacccctaa tccataatgg
    59521 ccaaatttct gtttagtttt tttgagcgtc tgtccacatt tgaagatatc ttcaggtttt
    59581 ggtctgaaag cagtagtgtt attaaaataa ttttcatata caggtgtggg tgctatagca
    59641 ttgcttctaa ttagatggag aagatgatca tccatgtgtt gtttatgttt tttattaaat
    59701 atatttgcta attcctcagg atcaattggg tctttcatct gattaaatgg aacctgcaaa
    59761 gcaccgagac aataacattg actttcttca tttcttatta ttccgtgatg actggttaaa
    59821 actttagttt ctgagttata attacaaaca tcgagcgttt taattaagtt atacgtatca
    59881 ccggcagaga gtttataatt ttctagtcgt gcagtatagt taccattaaa attgatttca
    59941 tgatttccag ccagaacgat gacattttta ttaatacggc tatctttatt accctccata
    60001 tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt actaaatcta
    60061 tcgcctgtat gatccccaag ggggatacaa ggggttatcg catcgtcctt taaaaaaaca
    60121 tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta gagcaattta
    60181 ttatttttat gtatgtatgc agatgaagcc atgtcttcgt gttttaataa ttcacataat
    60241 aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatga ataaagtaca
    60301 gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg tgggttaatc
    60361 acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata gctatcaaac
    60421 ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt aaaattaccg
    60481 ctaagaagag taaagaaatt ttcaggtatg atagaaagcc cattaacttt ataaattatg
    60541 tctgatttat ctccggagaa attaataaaa taataaccat ccttacactc aatcttaact
    60601 gtaatgggag acgataattt tatcacatgg ttattgtctg gtataattgt aaagataaac
    60661 gtgttagtag tattatcggc aaaactatta tattcttgaa tatctataga tggctcacta
    60721 ttgtaaaacc atattttctc catctgaaga ctatgttcat ttaccctcca gtgaccatgt
    60781 tgcaggaaat agatgatatg catgccaaaa tcgttatcta tttgcgaatc attatccagt
    60841 ttatcaattt caactatctt tgaacgatgt gttttatcaa ccttgctatg gtgttgatga
    60901 ttattaactt tatctgaatg cttatcttta tggtttgaag ggatgtactt aaatatattg
    60961 tcaattgctt tcataaataa cccgcgatat ttttgacctg ttagtaaaaa ataaatgata
    61021 catgtctgaa agacatattt acgacgatat aaaacaataa tgcttatatg ttcgctgatt
    61081 ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca tccctggaag
    61141 acaattgtaa aaaaaacaac agaataagaa ttaatcttag gataaatatt tatttatcat
    61201 ggccttttga ccgctaaaaa atggcactcg gcacagtaaa ccccgctata gttatgctat
    61261 acatttgtct ttaatgatat ctcgtcgtta tattaacagg ctgaaattat cattaatgca
    61321 catttcaata ttagcaggga tacctgagga gtttaaagtg aacatatata tcgggtagct
    61381 tttcaaatta atccctttga ttataggctt aatttgcatc gcgttaggtg gctttgtgct
    61441 ggaaagttca gggcaacgtc acgggtcatg tgctgatttc tctggcggcc atatgcctgg
    61501 cattattcac taccgcattt attatcattt cgcagctcac gcgcggcgtt aatacgtttt
    61561 acaatacatt gttccccatt attggctatg cggggtcaat tatcaccatg atatggggtt
    61621 gggcactgtt agcaggtaat gatgtgatgg cagacgagtt tgttgccggc catgttattt
    61681 ttggcgttgg tatgattgcc gcatgtgtat cgacggtggc agcgtcgtcc ggtcactttc
    61741 tgctcattcc caaaaatgca gcggggagca agagcgacgg aacaccggta caggcttatt
    61801 cttcattaat cggtaattgc ctcattgccg ttcccgtttt actcaccctg ctcggtttca
    61861 tttggtctat tacgctgtta catagtgccg acataactcc gcattatgtc gccttgggtt
    61921 aaccgcaatc tgtgcctgtc taattggcct agtggccaca atcgtccatc aaacacgtaa
    61981 tacgttctca tccaaagagc actggctgtg gtgttattgg gttatttttc tcggctcagt
    62041 cacggtgctg caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc
    62101 cggcattatt ctcatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct
    62161 actggcactg gtctggagac gtacctgttc gttagccaac agaataccga tgataccggt
    62221 atttacctgt ctgttttgct tgtttttgtc atcattcctc gcggaaatcg cacaaactga
    62281 gctggactac tttattccat ctcgggttct ggttggatta agcgcagtat gttttacatt
    62341 gttctctatt gtttcaatat tagaggccgg ttctgcgaaa aaaaattaaa atttacataa
    62401 tcccagcgtt attcatatct taacgctggt tttttacgaa cttagtgaaa taatgtagaa
    62461 tataaaatta taattttata ttggagcaca tgtgttaatg atcaaccccg ggatactaaa
    62521 aatatagaca ttattaaact tgcttagcct cgcattgcct ttacctgtta aagatgcatc
    62581 gagaaaattg agtctgaagt tctcatcctt tatctgattt tccagtcccg aaactttggc
    62641 cttagcatta cctgagatcc gtccgttact atctattttg taggtaaaaa aatacgaatg
    62701 ggaaaatttc ctcggtgtat cttcatcagt taagccaaca atcgtaaaac taccgctacc
    62761 atcttttttt aaaatgactg aaaggttagc agagtagtat aaagaatgat ctgttttagc
    62821 atttcttgtt aacgtcgacg tacattcaag agacgttgtg ctaccatcac gagccttcca
    62881 gagagcaaaa aaacacacct gtagcaaaaa tacattttct tattagtcat ataatagcac
    62941 ctctgaacaa attgcataag tttcgccatt agaggatttt atacatcttg ataaataccg
    63001 ctgcccttcc ccaagcaaat aatgcatact atttacatca tatataaaat ttgcacctgg
    63061 cacacagggt aaatgctttt tcgaaatttc agcggcctct atttgagatt tagcgaggcc
    63121 aatctcgtta agattcgctg aaagatataa atcacaggtg tcaatcttac ctgatttaac
    63181 caatggataa tccgttatac tgtaggtatc tttaaaagcc acggcaaaca tttcaataat
    63241 aacaattact accacaagcg aaatcaaaat gcgaaaaaga ggaatactcc ttttgcgctc
    63301 tactttttca acaacatctt cttgtaaatg agaatcatca taagattgtt tacttattgt
    63361 tatgttttca ctgatgtaaa agcctacttt gggtatcgta agaatcacat tttcttcaac
    63421 accaatattt ttaaaacttt tgcgtaacag ggatatatat tggttcaatg tgttgtttga
    63481 cagatcaaat gaaaatctct ccaaaacgct atcaatgatt gagttcctgt caaccgctcc
    63541 accattactt tccagtaaca ctgccaaaat acaccccgcc gtagctggca aatttatctc
    63601 atcctgggta tttacattcg agagcgagca tttgttcgga tcaaaaataa tcgcgtcatt
    63661 gataatgaaa aaacatctcc gctccatgca ggcaacaaca caaaataaga ttttgtcatt
    63721 ttagcctatt cctggtggaa ctgacacaag aaactaaaat acaaatataa ccctgaaaaa
    63781 gtcatataaa acttatatcc acagaataaa aaaacaaaac atgtcagttt tatcaatata
    63841 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
    63901 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
    63961 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
    64021 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
    64081 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
    64141 tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
    64201 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
    64261 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
    64321 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
    64381 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
    64441 atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca
    64501 gatatactct ctcttcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
    64561 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
    64621 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
    64681 attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt
    64741 ttttataagt taaaaaaggc gggatatcaa atcccgccat gaaattaact aatcatctgc
    64801 attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct
    64861 gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca
    64921 tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg
    64981 tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg
    65041 aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc
    65101 catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat
    65161 ataccccttt cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat
    65221 tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca aaagctgtcc
    65281 ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga ttttcattta
    65341 cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat gcttccacca
    65401 aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga aactataaac
    65461 atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt tggtattttg
    65521 cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct tccgcactca
    65581 agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt tcagtgtctt
    65641 tgctataact aatcacagcg ccgaagggga cgggtttacc ctctttctgt tttaaggtta
    65701 ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct ccagctgtcg
    65761 gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt ttttcatcca
    65821 gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca cgaaaatcag
    65881 tactcagtcc cggccagcca ttaacgttaa cccccgttgc tccgggtgcc tctataaggg
    65941 ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata agtatcccac
    66001 cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg ctactaaact
    66061 gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc ggttgtgact
    66121 gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta ttctgacctg
    66181 ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt gaagacgcat
    66241 aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt aatttctgat
    66301 ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg gagaatgtta
    66361 tgcctctcca taccgtacta taactcaggc tggtagaccg cgtgtttcct gtgccccggg
    66421 aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg ggctgggata
    66481 aggatatcgt agagccatcg tgactattct tatctctttt tctgttccgt tcatcctgtg
    66541 cacttactgc ttcagtcagt gtggcataac ctgatgcata acggttcgtg gttataga
//
LOCUS       DAJTXY010000008        65614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTXY010000008 DAJTXY010000000
VERSION     DAJTXY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.008"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(130..609)
                     /gene="folK"
                     /locus_tag="OO566_000611"
     CDS             complement(130..609)
                     /gene="folK"
                     /locus_tag="OO566_000611"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6604013.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="OO566_000612"
     CDS             complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="OO566_000612"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6604014.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="OO566_000613"
     CDS             complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="OO566_000613"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6604015.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="OO566_000614"
     CDS             complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="OO566_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6604016.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="OO566_000615"
     CDS             complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="OO566_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6604017.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="OO566_000616"
     CDS             complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="OO566_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6604018.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4982..7411
                     /gene="hrpB"
                     /locus_tag="OO566_000617"
     CDS             4982..7411
                     /gene="hrpB"
                     /locus_tag="OO566_000617"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6604019.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7613..10147
                     /gene="mrcB"
                     /locus_tag="OO566_000618"
     CDS             7613..10147
                     /gene="mrcB"
                     /locus_tag="OO566_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6604020.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLAAIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10367..12610
                     /gene="fhuA"
                     /locus_tag="OO566_000619"
     CDS             10367..12610
                     /gene="fhuA"
                     /locus_tag="OO566_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6604021.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FGENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12661..13458
                     /gene="fhuC"
                     /locus_tag="OO566_000620"
     CDS             12661..13458
                     /gene="fhuC"
                     /locus_tag="OO566_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6604022.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13458..14348
                     /gene="fhuD"
                     /locus_tag="OO566_000621"
     CDS             13458..14348
                     /gene="fhuD"
                     /locus_tag="OO566_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6604023.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14345..16327
                     /gene="fhuB"
                     /locus_tag="OO566_000622"
     CDS             14345..16327
                     /gene="fhuB"
                     /locus_tag="OO566_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6604024.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16362..17642)
                     /gene="hemL"
                     /locus_tag="OO566_000623"
     CDS             complement(16362..17642)
                     /gene="hemL"
                     /locus_tag="OO566_000623"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6604025.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLMTEL
                     VPTIDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17867..19288
                     /gene="clcA"
                     /locus_tag="OO566_000624"
     CDS             17867..19288
                     /gene="clcA"
                     /locus_tag="OO566_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6604026.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGVLVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19312..19377
                     /locus_tag="OO566_000625"
     CDS             19312..19377
                     /locus_tag="OO566_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604027.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19370..19714
                     /gene="erpA"
                     /locus_tag="OO566_000626"
     CDS             19370..19714
                     /gene="erpA"
                     /locus_tag="OO566_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6604028.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19761..20384)
                     /locus_tag="OO566_000627"
     CDS             complement(19761..20384)
                     /locus_tag="OO566_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6604029.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20422..21222)
                     /gene="btuF"
                     /locus_tag="OO566_000628"
     CDS             complement(20422..21222)
                     /gene="btuF"
                     /locus_tag="OO566_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6604030.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21215..21913)
                     /gene="mtnN"
                     /locus_tag="OO566_000629"
     CDS             complement(21215..21913)
                     /gene="mtnN"
                     /locus_tag="OO566_000629"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6604031.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21997..23514
                     /gene="dgt"
                     /locus_tag="OO566_000630"
     CDS             21997..23514
                     /gene="dgt"
                     /locus_tag="OO566_000630"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6604032.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23644..25068
                     /gene="degP"
                     /locus_tag="OO566_000631"
     CDS             23644..25068
                     /gene="degP"
                     /locus_tag="OO566_000631"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6604033.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25223..26379
                     /gene="cdaR"
                     /locus_tag="OO566_000632"
                     /pseudo
     CDS             25223..26379
                     /gene="cdaR"
                     /locus_tag="OO566_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26468..26854)
                     /locus_tag="OO566_000633"
     CDS             complement(26468..26854)
                     /locus_tag="OO566_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6604034.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27168..27992)
                     /gene="dapD"
                     /locus_tag="OO566_000634"
     CDS             complement(27168..27992)
                     /gene="dapD"
                     /locus_tag="OO566_000634"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6604035.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28023..30695)
                     /gene="glnD"
                     /locus_tag="OO566_000635"
     CDS             complement(28023..30695)
                     /gene="glnD"
                     /locus_tag="OO566_000635"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6604036.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30757..31551)
                     /gene="map"
                     /locus_tag="OO566_000636"
     CDS             complement(30757..31551)
                     /gene="map"
                     /locus_tag="OO566_000636"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6604037.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31919..32644
                     /gene="rpsB"
                     /locus_tag="OO566_000637"
     CDS             31919..32644
                     /gene="rpsB"
                     /locus_tag="OO566_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6604038.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32902..33753
                     /gene="tsf"
                     /locus_tag="OO566_000638"
     CDS             32902..33753
                     /gene="tsf"
                     /locus_tag="OO566_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6604039.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33900..34625
                     /gene="pyrH"
                     /locus_tag="OO566_000639"
     CDS             33900..34625
                     /gene="pyrH"
                     /locus_tag="OO566_000639"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6604040.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34917..35474
                     /gene="frr"
                     /locus_tag="OO566_000640"
     CDS             34917..35474
                     /gene="frr"
                     /locus_tag="OO566_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6604041.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35566..36762
                     /gene="ispC"
                     /locus_tag="OO566_000641"
     CDS             35566..36762
                     /gene="ispC"
                     /locus_tag="OO566_000641"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6604042.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36951..37709
                     /gene="ispU"
                     /locus_tag="OO566_000642"
                     /gene_synonym="uppS"
     CDS             36951..37709
                     /gene="ispU"
                     /locus_tag="OO566_000642"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6604043.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37722..38579
                     /gene="cdsA"
                     /locus_tag="OO566_000643"
     CDS             37722..38579
                     /gene="cdsA"
                     /locus_tag="OO566_000643"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6604044.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWCADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38591..39943
                     /gene="rseP"
                     /locus_tag="OO566_000644"
     CDS             38591..39943
                     /gene="rseP"
                     /locus_tag="OO566_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6604045.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39973..42405
                     /gene="bamA"
                     /locus_tag="OO566_000645"
     CDS             39973..42405
                     /gene="bamA"
                     /locus_tag="OO566_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6604046.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42527..43012
                     /gene="skp"
                     /locus_tag="OO566_000646"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42527..43012
                     /gene="skp"
                     /locus_tag="OO566_000646"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6604047.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43016..44041
                     /gene="lpxD"
                     /locus_tag="OO566_000647"
     CDS             43016..44041
                     /gene="lpxD"
                     /locus_tag="OO566_000647"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6604048.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44146..44601
                     /gene="fabZ"
                     /locus_tag="OO566_000648"
     CDS             44146..44601
                     /gene="fabZ"
                     /locus_tag="OO566_000648"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6604049.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44605..45393
                     /gene="lpxA"
                     /locus_tag="OO566_000649"
     CDS             44605..45393
                     /gene="lpxA"
                     /locus_tag="OO566_000649"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6604050.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45393..46541
                     /gene="lpxB"
                     /locus_tag="OO566_000650"
     CDS             45393..46541
                     /gene="lpxB"
                     /locus_tag="OO566_000650"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6604051.1"
                     /translation="MTDQRPLTIALVAGEISGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46538..47134
                     /gene="rnhB"
                     /locus_tag="OO566_000651"
     CDS             46538..47134
                     /gene="rnhB"
                     /locus_tag="OO566_000651"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6604052.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47171..50653
                     /gene="dnaE"
                     /locus_tag="OO566_000652"
     CDS             47171..50653
                     /gene="dnaE"
                     /locus_tag="OO566_000652"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6604053.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50666..51625
                     /gene="accA"
                     /locus_tag="OO566_000653"
     CDS             50666..51625
                     /gene="accA"
                     /locus_tag="OO566_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6604054.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51724..53865
                     /gene="ldcC"
                     /locus_tag="OO566_000654"
     CDS             51724..53865
                     /gene="ldcC"
                     /locus_tag="OO566_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6604055.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53922..54311
                     /locus_tag="OO566_000655"
     CDS             53922..54311
                     /locus_tag="OO566_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6604056.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54376..55674
                     /gene="tilS"
                     /locus_tag="OO566_000656"
     CDS             54376..55674
                     /gene="tilS"
                     /locus_tag="OO566_000656"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6604057.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55723..55983)
                     /gene="rof"
                     /locus_tag="OO566_000657"
     CDS             complement(55723..55983)
                     /gene="rof"
                     /locus_tag="OO566_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6604058.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55970..56170)
                     /locus_tag="OO566_000658"
     CDS             complement(55970..56170)
                     /locus_tag="OO566_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6604059.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56336..56869
                     /locus_tag="OO566_000659"
     CDS             56336..56869
                     /locus_tag="OO566_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6604060.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAW"
     gene            56878..57300
                     /gene="arfB"
                     /locus_tag="OO566_000660"
     CDS             56878..57300
                     /gene="arfB"
                     /locus_tag="OO566_000660"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6604061.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57314..58024
                     /gene="nlpE"
                     /locus_tag="OO566_000661"
                     /gene_synonym="cutF"
     CDS             57314..58024
                     /gene="nlpE"
                     /locus_tag="OO566_000661"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6604062.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58179..59003)
                     /locus_tag="OO566_000662"
     CDS             complement(58179..59003)
                     /locus_tag="OO566_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6604063.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRNIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59056..60774)
                     /gene="proS"
                     /locus_tag="OO566_000663"
     CDS             complement(59056..60774)
                     /gene="proS"
                     /locus_tag="OO566_000663"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6604064.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60885..61592)
                     /gene="tsaA"
                     /locus_tag="OO566_000664"
     CDS             complement(60885..61592)
                     /gene="tsaA"
                     /locus_tag="OO566_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6604065.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61589..61993)
                     /gene="rcsF"
                     /locus_tag="OO566_000665"
     CDS             complement(61589..61993)
                     /gene="rcsF"
                     /locus_tag="OO566_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6604066.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62111..62926)
                     /gene="metQ"
                     /locus_tag="OO566_000666"
     CDS             complement(62111..62926)
                     /gene="metQ"
                     /locus_tag="OO566_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6604067.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62966..63619)
                     /gene="metI"
                     /locus_tag="OO566_000667"
     CDS             complement(62966..63619)
                     /gene="metI"
                     /locus_tag="OO566_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6604068.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63612..64643)
                     /gene="metN"
                     /locus_tag="OO566_000668"
     CDS             complement(63612..64643)
                     /gene="metN"
                     /locus_tag="OO566_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6604069.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64831..65406
                     /gene="gmhB"
                     /locus_tag="OO566_000669"
     CDS             64831..65406
                     /gene="gmhB"
                     /locus_tag="OO566_000669"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6604070.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15511 a        16251 c        18082 g        15770 t
ORIGIN      
        1 gatgggattg atatttatct gcgggaattt atattatgtt tctgcaaaaa tgtttaaact
       61 aaaattttta ttaaggaaag agaggatgta tgattttcag aaaatattta tattcgtaat
      121 ataaataaat taccatttgc ttaatttgtc aaatgctctt gtatgtaaga cttcacgcaa
      181 cgtctcccca tcaggaaacg ccagctccgg tgcaatctca aacagcggcc acagcataaa
      241 tccacgattc ttcatatcgt agtgcggaac ggtcaggcgt tcggtattta tcacttcatt
      301 accaaacagc atgatatcga gatccagcgt gcgtggtccc cagcgttcag ctttacgaac
      361 acgaccctgt tgcagttcta tgcgctgtgt atggttgagt aactcttccg gggcgggcga
      421 agtttcaagc gccacggcgg cgttgaggta atcgggttga tcttgcggcc ccagcggtgg
      481 ggtgcggtaa aacgaagaaa cggcaagaat gcggctttca gggatatcgc ctaatgcttt
      541 cagggcagca ttgacctgct ccagcggaga ggccagattg ctgcctatgg caatatacgc
      601 cactgtcatg tggtaccctc acgacgcggt gcgcgtttgc gtggacgacg agtacgacga
      661 cgcggtgacg gttcttcatc cagctcgtta agcatccctt tttgatctgg tggtgcggaa
      721 acctggaact caccccacca tttcaccaga cgctgcagtt cagcattacg ttcaacttca
      781 gctcgcaagg ccaacaggtc ataagccgca cggaacttag gatgctccag cagtttccat
      841 gcgcgtttac cctgacgacg ggacatacgc aactgcaact gccagatatc gcgggttaac
      901 gtcgtcagac gtttcgggat tgccagtgaa cggcaggctt cgtccagcac gtcgttcatc
      961 gccagcgcga aagcgtcgtg ataggtcagg ccgctttcct gggcgatctt ctgtgccgtc
     1021 tccagcagtg ggtaccagaa catggcggca aacaagaacg ccgggttcac gcgcatatcg
     1081 ttatggatac gcgtatcggt attcttcagc acctgttcaa tgatccgctc catcgggctg
     1141 tcgccatttt ccgtgaagta gcgggtaatg gtcgggaaca gcggctggaa cagatgatat
     1201 tcacacaaca gcttataggt ttcgtaaccg tagcccgctt gtagcagttt aagcgattct
     1261 tcaaacaggc gtgccggtgg gatatcgttc agcagggtag cgaggcgagg gatcggttct
     1321 gcggtttccg ggctgatgcg catacccaat ttggcggcaa aacgtaccgc gcgcagcata
     1381 cgtaccggat cttcacggta gcgcgtttcc gggttaccaa tcagacggat aacgccgtcc
     1441 ttcagatcct tcataccgcc aacgtaatca cggacggtaa aatccgctac gctgtaatac
     1501 aggctgttga tagtgaaatc gcggcgctgg gcgtcttctt cgatggagcc gaaaatgttg
     1561 tcgcgcagca acatgccgtt ttgcccgcgt tgggaggtcg tgcggtcgct gacgttacct
     1621 tcgtggtgtc cacggaaggt cgcaacttcg ataatctccg ggccaaacat tacatgagcc
     1681 agacggaaac ggcgacccac caggcggcag ttacggaaca gtttgcgcac ctgctcaggc
     1741 gtggcgttag tggttacgtc aaaatctttc ggctttttgc caagtaacag gtcgcgcacg
     1801 ccgccgccaa ccagccaggc ttcgtatccc gctttgttga gcctgtacat taccttcagg
     1861 gcattttcac tgatatcttt gcgggaaata gcatgctgct cacgcgggat caccgtcacc
     1921 tgtggacggg cgactgcctg ttcagcctcg ctttcctcgc ggcttagcac cttgcggcaa
     1981 aaattagcga ctcgggtaaa aatagtacac ctcggtagtg tcaaacatca ttcaggacaa
     2041 aaaaatagcg gctaatcata gctcagcatg acgcatttga gaatgttgaa tttacaattg
     2101 ccgactcggg cacggcggtc agcatccagt ttttgacggc tgactgaagg atttgctcga
     2161 cgctgaaatc ctgccagtgt gcttctgcct gctgccccag aaattgaagt gccgcgatta
     2221 gtaccgggcg tggatcgcct ttcggcaacg caggcgcatg attctgcttg gaaagtttag
     2281 cgccttgtgg attaagcgcc agcggcagat gaatgtaatc tggcactttc cagccaaaaa
     2341 gctggtacag cgagatttgc cttactgttg gttcaatcag atcagcccca cgcactattt
     2401 ctgtaacgcc ctggaaatga tcatcaacca caacagccag gttgtaggcg aacaacccat
     2461 cacggcgatg aatgataaaa tcttcccgtg ccagtttttc gtcggcgtga ataatgcctc
     2521 gcagcaggtc agtaaattgc gtgaccggat gctgctggcg gatacgcact gcggcgttgt
     2581 ctggtccatg atgcaacacc cggcaatgac cgtcgtaaat accgccaatg ctttgaatac
     2641 gcgcacgcgt acaggtgcag taataactta gtccttgttc atgtaaccag gcgagtgctt
     2701 cacgataggc gtcgtgacgt tgcgattgcc agagaacatc gccgtcccag tgcagaccgt
     2761 aatgttccag ctggcgcagg atagtttctg cggcaccggg aacttcacga ggcgggtcga
     2821 tatcttctat gcgtaccagc cagcgacctt gccgggcgcg accctgcaaa tagctgccga
     2881 gcgcggcgat cagagagcca aaatgaagct cgccggaagg agagggggcg aagcggccaa
     2941 tatactgtgt gtctgtcatc tctttgaaca aaaaataagg cgggagcatt tcccgcctgt
     3001 ggtaaacatg atggaacggc tgtaattagc cagccatctg tttttcgcga atttcagcca
     3061 gcgttttgca gtcgatgcac agatcggctg tcgggcgcgc ttccagacgg cgaataccaa
     3121 tttcaacacc gcaggattcg cagtagccga aatcttcgtc ttccactttt ttcagcgtct
     3181 tctcgatctt tttgatcagc ttacgctcgc gatcgcggtt acgcagttcg aggctgaact
     3241 cttcttcctg ggctgcacgg tctaccgggt ccgggaagtt ggctgcttca tcctgcatat
     3301 gtgtaacggt gcgatcgact tcatccctga gttgattacg ccatgcttcc agaatacgac
     3361 ggaagtgcgc cagctgggct tcattcatat actcttcgcc cggcttctcc tgatatggtt
     3421 ccaccccagc gatggcgaga atactcaggg acgatgtttt acggttttgc ccttcttgca
     3481 tgttgcttct ccttaacacg cactatcgat ccccatgttc gggggaaaaa tgaggccgct
     3541 ataaatagca gatgcttttc cggatagcaa ttatctaaac gtaacacttg acaactgtgt
     3601 gaggaaaagc gtattcgcgc acgcgaccag aatgtaaatt aaccagttac ttactttact
     3661 acaatgtaac cggcagtgat tttttaagag ccatgccttc agcagaaatt tccgctttgt
     3721 aagccagaat ttctaccccc ctctgttgag cttctgacaa tagttgcgcg tatttctcat
     3781 cgatgtggcg cgcgggtgaa aaccgtgtaa tggctgaatg cagcacggcg aaaaagataa
     3841 ccgcacgctg gccttcagcc gctacgctca tcaactcccg aaggtgtttc tgacctcgtt
     3901 cagtgaccgc atcgggaaaa tatccctgtt cgttctccgc taacgtaacc gatttcactt
     3961 caatatagca gtctggacgc gaatccgcct gcaacataaa gtcaatacgg ctgcgttcgg
     4021 agccgtattt tacttcgctt ttcagcgagc tatagcctga cagttctgaa attgattcat
     4081 taaggatagc ctctttcgtc aacctgttag cccaaagcgt attgacgcaa ataaatgcgc
     4141 cgctctggct ttgagttaat tcccaggtgt gtgggtattt ccgtttggtg ttgtctgaag
     4201 tcgaatacca gacggtatcg ccaggcgttg cacaaccggt catcgcaccc gtattcgggc
     4261 agtgtagcgt taattcgcga ccatcgggtg tgatcacatc ggctaaaaaa cgtttgtaac
     4321 gctgaattag cgtcgcgcgc tgtagagggg gagaaaattc cattgcgaca atccttgtta
     4381 ttgcgttagc gcccagcgtt ttagcggcgt gtagcgtgtg cgtccacggg caaacgagga
     4441 ggcgtaaagg gtgaactccg tcaccgcata agaccagtta aaacccggcg gcgggaatgt
     4501 caccgcctcg ctggcgtcgc gcaataaggt aatatgtgga tgaaacggac gattgctttg
     4561 aaaacaaccg ctgcgggcag cctgtgaacg gagcatattc gccagctgga ttaagccgcg
     4621 tggaggctga cgcatcccta accacaccac acgcgaacgc agccattgtc cggcgtcatc
     4681 aagcgtgagt gtgaaacctg gttgacgaat ccgtccggct aaaagagaaa gcgccttctc
     4741 tttctctgcg ctcacttcgc ctaaaaatgc cagagtcaga tgcaaattat cggcggcgac
     4801 cggacgtccc gcctcaggtg ggaagtgtgt ggcgcgccaa tggataatct gttcgcgggt
     4861 ttctgcaggt aagtcgatag caaagaacag acgttgcggt tcagacatgt gaggcactcg
     4921 gttatgaatt accgcgatgc tacaatgtgg cgcgaagaat gttaaccctc tggagcgttt
     4981 tgtgtcgtcg ttgcccgttg ctgccgtctt acctgaatta cttaccgctc tcgattgtgc
     5041 gccgcaggta ttattaagtg cgccgaccgg ggccgggaaa tcaacctggc tgccgctgca
     5101 actgctggcg catcccggca ttaacgggaa aattatcctg ctggagccgc gtcgtctggc
     5161 ggcgcgtaac gtcgcgcaac ggctggcgga gctgcttaac gaaaagccag gcgataccgt
     5221 tggctaccgg atgcgtgcgc aaaactgcgt cgggccgaat acccgcctgg aagtggttac
     5281 cgaaggcgtg ctgacgcgga tgatccagcg tgacccggaa ctgagcggtg ttggattggt
     5341 gatccttgat gagtttcatg agcgcagttt gcaggcggat ttggcgttgg cgctgttact
     5401 cgatgtgcaa caaggtctgc gtgatgacct taaactgctg attatgtcgg ctacgctgga
     5461 caatgaccgc ttgcagcaaa tgctgccgga agcgcccgtc gtcatctcag aagggcgctc
     5521 gtttccggtt gaacgccgtt atttaccgct gcccgcgcat cagcgttttg acgaagccgt
     5581 tgcggtagcc accgccgaaa tggtgcgtca ggaaagtgga tcattactgt tatttttacc
     5641 tggcgtcgga gaaattcagc gcgtgcagga acaactggct tcgcgcatcg gcagtgatgt
     5701 ggtgctctgc ccgctgtatg gcgcgttgtc actgaacgat cagcgaaaag cgatcctccc
     5761 ggcaccgcaa gggatgcgca aagtggtgct ggcgaccaat attgctgaaa ccagtttaac
     5821 catcgaaggt attcgtctgg tggtggattg tgcccaggag cgtgtggcgc gctttgatcc
     5881 gcgcacgggg cttacgcggt tgattactca acgcgttagt caggcatcca tgacgcagcg
     5941 tgccgggcgc gccgggcgtc tggagccggg tatcagcctg catttaatcg ccaaagaaca
     6001 agcagaacgc gccgcggcgc aaagtgagcc ggagatctta caaagcgatc tttccggttt
     6061 gctgatggaa ttactgcaat ggggatgcag cgatccggcg cagatgagct ggctggatca
     6121 accgccaacg gtgaatctac tggccgcgaa acgcctgtta caaatgctgg gggcgctgga
     6181 gggtgaacgg cttagtgcgc aagggcaaaa aatggcagcg ctgggtaacg atccgcgttt
     6241 agcggcaatg ctggttaacg caaagagcga tgacgaagct gctaccgcgg caaaaattgc
     6301 cgccattctc gaagagccgc cacggatggg caatagtgac ctgggcgtgg cgttttcgcg
     6361 caatcaacca gcctggcagc aacgtagtca gcaactgtta aaacgcttaa acgtacgcgg
     6421 cggtgaggca gacagttcgc ttatcgcgcc gctacttgcc ggggcgtttg ccgatcgcat
     6481 tgctcatcgc cgtgggcaag atggacgcta tcaactggca aacggcatgg gagcgatgct
     6541 cgatgccgac gacgcgctaa gccgccacga atggttgatc gcaccgttat tattgcaggg
     6601 cagcgcctcg ccggatgcgc ggattttact ggcgctaccg gtcgatattg atgaattagt
     6661 acaacgctgt ccgcagctgg tgcagcaatc tgacaccgtg gaatgggatg acgcgcaagg
     6721 tacgctgaaa gcctggcgtc ggctacaaat cggtcagttg acggtgaaag tgcagccgct
     6781 ggcgaaaccg tcagaagacg agttgcatca ggcgatgctt aatggcatcc gtgataaagg
     6841 tttaagcgtg ctcaactgga cggcggaagc ggaacagcta cgcttgcgtt tgttatgcgc
     6901 cgcaaagtgg ttgccggaat atgactggcc agcggttgat gatgaaagtt tgttggcgac
     6961 gctggaaacg tggctgctgc cacatatgac cggcgtacat tcactacgcg gcctgaaatc
     7021 actcgacatt tatcaggcac tacgcggatt acttgattgg ggaatgcagc aacgtctgga
     7081 tagtgaattg cctgcgcatt acactgtgcc gacgggaagc cggatcgcca ttcgttatca
     7141 tgaagataac ccgcccgcgc tggcggtgag aatgcaggag atgtttggtg aggccactaa
     7201 tccgacgatc gcccaggggc gcgtgccgct ggtgctgaag ttgctttccc ctgcccaaag
     7261 gccgctgcaa atcacgcgtg atttgggggc cttctggaaa ggggcgtatc gtgaggtgca
     7321 aaaggagatg aaagggcgtt atcccaaaca cgtctggccg aacgacccgg caaacacggc
     7381 accgacgcga cggacgaaaa agtattcgta aggttggttt tctccctctc cctgtgggag
     7441 agggccgggg tgagggcatc agcgcgcacg ttcaccctca ccctaaccct aaccctctcc
     7501 ctcaagggag aggggaccga tcgagcacaa attttgagag atatcttctt ctgtcttgta
     7561 acagaagaac agaaaatcgg gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg
     7621 gaatgaccgc gagccaattg gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt
     7681 aagccgtcgt cgttacgaag atgacgatga ttacgacgat tatgatgact atgaggatga
     7741 agaaccgatg ccgcgcaaag gtaagggcaa aggcaaaggg cgtaagcctc gtggcaaacg
     7801 cggctggcta tggctactgc taaaactggc tatcgttttt gccgtgctga tcgccattta
     7861 cggcgtttat ctcgatcaaa aaattcgtag ccgtattgat ggcaaggtct ggcaactgcc
     7921 cgcggcagtt tatggccgaa tggtcaacct tgaaccagat atgaccatca gcaagaacga
     7981 aatggtgaag ctgctggagg caacacagta tcgtcaggtg tcgaaaatga cccgtcctgg
     8041 cgaatttacc gtgcaggcca acagcattga gatgatccgc cgtccgtttg atttcccgga
     8101 cagtaaagaa gggcaggtgc gtgcgcgtct gacctttgat ggtgatcatc tggcggccat
     8161 cgtcaatatg gagaacaacc gtcagttcgg tttcttccgt cttgatccgc gtctgatcac
     8221 catgatctcc tcgccaaacg gtgagcagcg tttgtttgtg ccgcgcagtg gtttccctga
     8281 tttgctggtg gatactttgc tggcgacaga agaccgtcat ttttacgagc atgatggaat
     8341 cagtctctac tccattgggc gtgcggtgct ggcaaacctg accgcaggtc gcacggtgca
     8401 gggtgcgagt acgctgacgc aacagctggt gaaaaacctg ttcctctcca gcgagcgttc
     8461 ttactggcgt aaagcgaacg aagcttacat ggcgctgatc atggacgcgc gttacagcaa
     8521 agaccgtatt cttgagctgt atatgaacga ggtgtatctc ggtcagagcg gcgacaacga
     8581 aatccgcggc ttcccgctgg caagcttgta ttactttggt cgcccggtag aagagctaag
     8641 cctcgaccag caggcgctgt tagtcggtat ggtgaaaggg gcgtccatct acaacccgtg
     8701 gcgtaacccg aaactggcgc tggagcgacg taatctggtg ctgcgtctgc tgcaacagca
     8761 acagattatt gatcaagaac tctatgacat gttgagtgcc cgtccgctgg gggttcagcc
     8821 gcgtggtggg gtgatctctc ctcagccagc ctttatgcag ttggtgcgtc aggagttgca
     8881 ggcaaaactg ggcgataagg taaaagatct ctccggcgtg aagatcttca ctacctttga
     8941 ctcggtggcc caggacgcgg cagaaaaagc cgccgtggaa ggcattccgg cactgaagaa
     9001 acagcgtaag ttgagcgatc ttgaaactgc gattgtggtc gtcgaccgct ttagtggtga
     9061 agttcgtgcg atggtcggag gttctgagcc gcagtttgcg ggctacaacc gtgcgatgca
     9121 ggcgcgtcgt tcgattggtt cccttgcaaa accagcgact tatctgacgg ctttaagcca
     9181 gccgaaaatc tatcgcctga acacgtggat tgccgatgcg ccaatcgcgc tgcgtcagcc
     9241 gaatggccag gtctggtcac cgcagaacga tgaccgtcgt tatagcgaaa gcggcagagt
     9301 gatgctggtg gatgcgttga cacgttcgat gaacgtgccg acggtaaatc tggggatggc
     9361 gctggggctg cctgcggtta cggagacctg gatcaaactg ggcgtaccga aagatcagtt
     9421 gcatccggtt ccggcaatgc tgctgggggc gttgaactta acgccaatcg aagtggcgca
     9481 ggcattccag accatcgcca gcggtggtaa ccgtgcaccg ctttctgcac tgcgttcggt
     9541 aatcgcggaa gatggtaagg tgctgtatca gagcttcccg caggcagaac gcgctgttcc
     9601 ggcgcaggcg gcgtatctga cactatggac catgcagcag gtggtacaac gtggtacggg
     9661 tcgtcagctt ggggcgaaat acccgaacct gcatctggca gggaaaacag ggactaccaa
     9721 caataacgta gatacctggt ttgcaggcat tgacggcagc acggtgacca tcacctgggt
     9781 aggccgtgat aacaaccagc cgaccaaact gtatggagcc agcggggcaa tgtcgattta
     9841 tcagcgttat ctggctaacc agacgccaac gccgctgaat cttgttccgc cagaagatat
     9901 tgcagatatg ggcgtggact acgacggcaa ctttgtttgc agcggtggca tgcgtgtctt
     9961 gccggtctgg accagcgatc cgcaatcgct gtgccagcag agcgagatgc agcagcagcc
    10021 gtcaggcaat ccgtttgatc agtcttctca gccgcagcaa cagccgcaac agcaacctgc
    10081 tcagcaagag cagaaagaca gcgacggtgt agccggttgg atcaaggata tgtttggtag
    10141 taattaacat ctaagcgtga aataccggat ggcgagttgc catccggtaa aataacatcc
    10201 catctaagat attaaccctt tcttttcatc tggttgttta ttaacccttc aggaacgctc
    10261 agattgcgta ccgcttgcga acccgccagc gtttcgaata ttatcttatc tttataataa
    10321 tcattctcgt ttacgttatc attcacttta catcagagat ataccaatgg cgcgttccaa
    10381 aactgctcag ccaaaacact cactgcgtaa aatcgcagtt gtagtagcca cagcggttag
    10441 cggcatgtct gtttatgcac aggcagcggt tgaaccgaaa gaagacacta tcaccgttac
    10501 cgctgcacct gcgccgcaag aaagcgcatg ggggcctgct gcaactattg cggcgcggca
    10561 gtcagctacc ggcactaaaa ccgatacgcc gattcaaaaa gtgccacagt ctatttctgt
    10621 tgtgaccgcc gaagagatgg cgctgcatca gccgaagtcg gtaaaagaag cgcttagcta
    10681 cacgccgggt gtctctgttg gtacgcgtgg cgcatccaac acctatgacc acctgatcat
    10741 tcgcggtttt gcggcagaag gccaaagcca aaataactat ctgaatggcc tgaagttgca
    10801 gggcaacttc tataacgatg cggtcattga cccgtatatg ctggaacgcg ctgaaattat
    10861 gcgtggcccg gtttccgtgc tttacggtaa aagcagtcct ggcggtctgt tgaatatggt
    10921 cagcaagcgt ccgaccaccg aaccgctgaa agaagttcag tttaaagccg gtactgacag
    10981 cctgttccag actggttttg actttagcga tgcgttggat gatgacggtg tttactctta
    11041 tcgcctgacc ggtcttgcgc gttctgccaa tgcccagcag aaagggtcag aagagcagcg
    11101 ttatgctatt gcaccggcgt tcacctggcg tccggatgat aaaaccaatt tcaccttcct
    11161 ttcttacttc cagaacgagc cggaaaccgg ttattacggc tggttgccga aagagggaac
    11221 cgttgagccg ctgccgaacg gtaagcgtct gccgacagac tttaatgaag gggcgaagaa
    11281 caacacctat tctcgtaatg agaagatggt cggctacagc ttcgatcacg aatttaacga
    11341 cacctttact gtgcgtcaga acctgcgctt tggtgaaaac aaaacctcgc aaaacagcgt
    11401 ttatggttac ggcgtctgct ccgatccggc gaatgcttac agcaaacagt gtgcggcatt
    11461 agcgccagcg gataaaggcc attatctggc acgtaaatac gtcgttgatg atgagaagct
    11521 gcaaaacttc tccgttgata cccagttgca gagcaagttt gccactggcg atatcgacca
    11581 caccctgctg accggtgtcg actttatgcg tatgcgtaat gacatcaacg cctggtttgg
    11641 ttacgacgac tctgtgccac tgctcaatct gtacaatccg gtgaatcccg atttcgactt
    11701 caatgccaaa gatccggcaa actccggccc ttaccgcatt ctgaataaac agaaacaaac
    11761 gggcgtttat gttcaggatc aggcgcagtg ggataaagtg ctggtcaccc taggcggtcg
    11821 ttatgactgg gcagatcaag aatctcttaa ccgcgttgct gggacgaccg ataaacgtga
    11881 tgacaaacag tttacctggc gtggtggtgt taactacctg tttgataatg gtgtaacacc
    11941 ttacttcagc tatagcgaat cgtttgaacc ttcttcgcaa gttgggaagg atggtaatat
    12001 tttcgcaccg tctaaaggta agcagtatga agtcggcgtg aaatatgtac cggaagatcg
    12061 tccgattgta gttactggtg ccgtgtataa tctcactaaa accaacaacc tgatggcgga
    12121 ccctgagggt tccttcttct cggttgaagg tggcgagatc cgcgcacgtg gcgtagaaat
    12181 cgaagcgaaa gcggcgctgt cggcgagtgt taacgtagtc ggttcttata cttacaccga
    12241 tgcggaatac accaccgata ctacctataa aggcaatacg cctgcacagg tgccaaaaca
    12301 catggcttcg ctgtgggctg actatacctt ctttgacggt ccgctttcag gtttgacgct
    12361 gggcaccggt ggtcgttata ctggctccag ctatggtgat ccggctaact cctttaaagt
    12421 gggaagttat acggtcgtgg atgcgttagt gcgttatgat ctggcgcgag tcggcatggc
    12481 gggctccaac gtggcgctgc atgttaacaa cctgttcgat cgtgaatacg tcgccagctg
    12541 ctttaacact tatggctgct tctggggcgc agaacgtcag gtcgttgcaa ccgcaacctt
    12601 tcgtttctaa tttctctttt ggggcacgga tttccgtgcc catttcacaa gttggctgtt
    12661 atgcaggaat acacgaatca ttccgatacc acttttgcac tgcgtaatat ctcctttcgt
    12721 gtgcccgggc gcacgctttt gcatccgctg tcgttaacct ttcctgccgg gaaagtgacc
    12781 ggtctgattg gtcacaacgg ttctggtaaa tccactctgc tcaaaatgct tggtcgtcat
    12841 cagcctccgt cagaagggga gattcttctt gatgcccagc cgctggaaag ctggagcagc
    12901 aaagcgtttg cccgcaaagt ggcttatttg ccgcagcagc ttcctccggc agaagggatg
    12961 accgtgcgtg aactggtggc gattggtcgt tacccgtggc atggcgcgct ggggcgcttt
    13021 ggtgcggcag atcgcgaaaa ggtcgaggaa gctatctcgc tggttggctt aaaaccgctg
    13081 gcgcatcggc tggtcgatag tctctctggc ggcgaacgtc agcgggcgtg gatcgccatg
    13141 ctggtggcgc aggatagccg ttgtctgttg ctcgacgaac cgacttcggc gctggatatc
    13201 gcccaccagg ttgatgtgct ggcgctggtg caccgtttaa gtcaggaacg tggcctgacg
    13261 gtcattgccg tgttgcacga tatcaatatg gcggcacgct actgtgatta tctggtcgcc
    13321 ctgcgcagtg gtgaaatgat tgctcaggga acgcctgcgg aaattatgcg cggcgaaacc
    13381 ctcgaaatga tttatggcat cccgatgggt attttgccgc atccggcggg tgctgcacct
    13441 gtgagttttg tttattgatg agcggcttac ctcttatttc gcgccgtcga ctgttaacgg
    13501 cgatggcgct ttctccgttg ttatggcaga tgaataccgc ccacgcggcg gctattgacc
    13561 ccaatcgtat tgtggcgctg gagtggttgc cggtggaatt actgctggcg ctcggcatcg
    13621 tgccttacgg cgtggcggat accatcaact atcgcctgtg ggtcagcgaa ccaccattgc
    13681 cggactcagt gattgacgtc ggtttgcgta cagaacctaa ccttgaactg ctgaccgaaa
    13741 tgaaaccgtc gtttatggtc tggtcggcag gatatggccc ttcatcagaa atgctggcac
    13801 gtattgcgcc gggtcgcgga tttaacttca gtgacggcaa acatccgttg gcgatggcgc
    13861 gtaaatcgct gacggaaatg gcagatttac ttaacctgca aagcgcagcg gaaacgcatt
    13921 tagcgcaata cgaagacttt atccgcagca tgaaaccccg ctttgtgaag cgtggtgcac
    13981 gcccgttact gctgacgacg cttatcgatc cgcgccatat gctggtcttc ggtccaaaca
    14041 gcttgttcca ggaaattctc gatgagtacg gcatcccaaa tgcctggcaa ggggaaacca
    14101 acttctgggg cagtaccgcc gtcagtatcg atcgtctggc agcgtataaa gacgttgatg
    14161 tgctctgttt tgatcacgac aacagcaaag acatggatgc gctaatggca accccgctgt
    14221 ggcaggccat gccgtttgtc cgtacagggc gctttcagcg cgtacccgca gtctggtttt
    14281 atggtgcaac gctttcggca atgcactttg tgcgtgttct ggataacgcc atcggaggta
    14341 aagcgtgagt aaacgaattg cgcttttccc ggcgttattg ctggcgctgt tagtgattgt
    14401 cgctacggcg ctcacctgga tgaacttctc acaggcgctg ccgcgcagtc agtgggcgca
    14461 ggccgcctgg tcgccggata ttgacgtcat cgagcagatg atttttcact acagcttgtt
    14521 gccgcgtctg gcgatttcgc tgctggtggg cgcgggcctg gggctggtgg gcgtgctgtt
    14581 tcagcaagta ctgcgtaacc cgctggcgga accgacgacg ttgggcgttg caacaggcgc
    14641 gcaactgggg attaccgtca ccacgctctg ggcgatccct agcgcgatgg cgagccagtt
    14701 tgctgcgctg gcaggggctt gcgttgttgg cttaattgtc tttggcgtcg cgtgggggaa
    14761 acggctgtcg ccggtaacgc tgattctcgc ggggttggta gtgagccttt attgcggcgc
    14821 aatcaatcag ttactggtta tcttccatca tgaccagttg caaagcatgt ttctgtggag
    14881 cactggaacg ctgacgcaaa ccgactgggg cggtgttgag cgtttatggc cgcagttgct
    14941 gggcggtgtg atgctgacgt tgctgctact tcgtccgtta accctgatgg ggcttgatga
    15001 tggcgtggcg cgcaatctcg ggctggcctt gtcgcttgcg cgtctggcgg cgctgtcgct
    15061 ggcgattgtc atcagtgcgc tgctggtgaa cgctgtgggg attatcggct ttatcgggtt
    15121 gttcgcgccg ctgctggcaa aaatgctggg ggcgcggcgt ctgctgccac gactgatgct
    15181 ggcgtcgttg attggtgcgc tgatcctctg gctttccgat caaatcatcc tctggctgac
    15241 tcgcgtgtgg atggaagtgt ccaccggttc ggtcactgcg ttgatcggtg cgccgctgct
    15301 actgtggctg ctgccgcgtt tacgcagcat tagcgcgccg gatatgaagg ttaacgatcg
    15361 tgtcgcgact gaacgccaac atgtgctggc gtttgccctc gcgggcggcg tgctgttgtt
    15421 gatggctgtg gtggtggcgc tgtcgtttgg tcgtgatgcg cacggctgga cgtgggcgag
    15481 cggggcgttg ctcgaggatt taatgccctg gcgctggccg cgaattatgg cggcgctgtt
    15541 tgcgggcgtc atgctggcgg tagcgggctg tattattcag cgactgaccg gcaacccgat
    15601 ggcaagcccg gaagtgctgg ggattagctc cggcgcggcg tttggtgtgg tgttaatgct
    15661 gtttctggta ccgggtaatg cctttggctg gttgttaccc gcaggcagtc tcggggcggc
    15721 ggtgatgctg ttgatcatta tgatcgccgc cggacgcggt ggattttccc cacaccgcat
    15781 gttactggcg gggatggcgt taagtaccgc gttcaccatg cttttgatga tgttgcaggc
    15841 aagtggtgac ccgcgaatgg cgcaagtgct gacctggatt tccggttcga cctacaacgc
    15901 gaccgatgct caggtctggc gcaccggaat tgtgatggtg attttgctgg cgattactcc
    15961 gctgtgccgc cgctggctga ccattttacc gctgggtggt gataccgcgc gggcggtagg
    16021 gatggcgctg acgccgacgc gaattgcgct gctgctgtta gcggcttgcc tgacggcgac
    16081 cgcgacgatg actattggac cgttgagttt tgttggttta atggcaccgc atattgcgcg
    16141 gatgatgggc tttcgacgga cgatgccaca tatcgtaatt tcggcgctgg tgggtggttt
    16201 actgctggtg ttcgctgact ggtgtgggcg gatggtgctg tttccattcc agatcccggc
    16261 ggggctgctg tcgaccttta tcggtgcgcc atattttatc tatttgctga gaaagcagag
    16321 ccgttaatag aatgcctcgc ccggtgggcg aggcaaaatt atcacaactt cgcgaacacc
    16381 cgacgtgcag catcgatggt gttattgata tcttccatgc tgtgcgccac agacataaag
    16441 cccgcttcaa acgctgacgg tgccaggtaa acaccttcat ccagcatcat atggaagaaa
    16501 cgcttaaaac gctcgacgtc acaggccatc acatcctgat agcacgtcac ggactcggcg
    16561 tcggtaaaga agataccgaa cataccaccg acgtggttaa cgaccagcgg aattcccgct
    16621 tcttctgccg cttccagcag accttctgcc agacgtgttg tcagctcatc cagcgtttcg
    16681 tgaatgcccg gctgcgcgac ttcattcaga caggcgaaac ccgctgccat cgcaatcggg
    16741 ttaccggaaa gcgtacccgc ctgatagacc ggacctgtag gggccagcgc atccattaca
    16801 tcacgacgac caccgaatgc gcctaccggc attccaccgc cgatgatttt tccgaggcag
    16861 gttaaatctg gcactacgcc gaaataatcc tgtgcgccag ctagcgctac gcggaaaccg
    16921 gtcatcactt catcgatgat cagcaacgcg ccaaattcgt cgcacagcgc acgcagacct
    16981 ggcaggaact ctggcagcgg cggaacacag ttcatattgc ctgccaccgg ctcgacgata
    17041 atacaggcaa tctcttgcgg gtattgctca aacgcggcgc gtacagaggc cagatcgtta
    17101 taagtacagg ttaaggtatg tttggcgaaa tctgccggaa cgcccggcga gtttggctgg
    17161 cctaacgtga gtgcgccaga accggctttc accagcaggc agtcagcgtg accgtggtaa
    17221 caaccttcaa atttaatgat tttgtcgcga ccggtaaaac cacgggccaa gcggatggcg
    17281 ctcatcgtcg cctcggtgcc ggagttcacc atgcgcacca tatctatggt cgggaccagt
    17341 tcggtcatca gttgcgccat tttcacttcc atttcggtcg gtgcaccaaa gcttaaacca
    17401 cgctcggcgg cttcaatcac ggcattgcgg atcgccggat ggttatggcc cagcaccatc
    17461 ggcccccagg aaccgacata atcgatatag gctttgccat caacatcgta cagataagcg
    17521 ccgtccgctt tttcgataaa cagtggagtg ccgcccacgc cagtaaaggc gcgaacaggg
    17581 gagttcacac cgccagggat cagctcgcgc gctgcgctgt aaagattttc agacttactc
    17641 atggagggtt cctgattcgt agaaaaagtg aatggctgct attctatgtt attcataaca
    17701 agttaaatac tcgtcaaaca tcaggctgct tgtactggtg caatcctgat ttcttagagt
    17761 ataaaagttt tgtgcatttg aaacattacg ctttgcaaag gattttcatg gaacgtgcga
    17821 gtaaaatgcc gtcatcttat ttgtatgacc aataagtgat cattggatga aaactgatac
    17881 tccctcttta gaaacaccgc aggccgcgcg cctgcgacgc agacaactga ttcgccaact
    17941 tcttgagcgc gataaaaccc cgttagccat tttgtttatg gcggcagtcg tcggcacgct
    18001 tatcgggctg gctgcggttg cttttgacaa aggtgtcgcc tggttgcaga accaacgtat
    18061 gggagtgctg gtacatactg ctgataatta tccgctactg ttaaccgtcg cttttctctg
    18121 ttcggcggtg ctggcgatgt tcggctactt tttggtgcgc aaatacgcgc ccgaagcagg
    18181 gggatcgggg atcccggaaa ttgaaggggc gctggaagat caacgtcctg ttcgctggtg
    18241 gcgtgtattg ccggtgaaat tctttggcgg gctggggaca ctcggcggag gcatggtgct
    18301 agggcgcgaa ggaccaaccg tgcagatcgg cggcaatatc ggtcgcatgg tgcttgatat
    18361 tttccgcctg aaaggtgatg aagctcgcca tacgctgctg gcaaccggtg ctgctgcggg
    18421 gctggctgcg gcctttaacg cgccgctggc gggtattttg tttattatcg aagagatgcg
    18481 tccgcagttt cgctatacgt taatttcgat taaagcggta tttattggcg tcattatgtc
    18541 gaccattatg taccggattt ttaatcatga agttgcgttg attgacgtcg gtaaactttc
    18601 tgatgcgccg cttaatacgc tgtggcttta tctgatcctc ggtattattt ttggcatttt
    18661 cggccctatt tttaataaat gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg
    18721 cggcaatatt accaaatggg tactgatggg gggggcgatt ggcggtctgt gtggattgct
    18781 ggggtttgtg gcaccagcaa cgtcgggcgg cggttttaac ctgattccta tcgctaccgc
    18841 ggggaatttc agcatgggaa tgctggtgtt tatcttcgtc gctcgggtca ttaccacctt
    18901 actctgcttc tcttccggcg cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac
    18961 tgtgctggga accgctttcg gaatggttgc cgttgagctg tttccgcaat atcacctgga
    19021 ggcggggacg tttgctattg ccggaatggg ggcattactg gcggcatcta ttcgcgcgcc
    19081 gttaacgggg atcattctgg ttctggagat gaccgataac taccagctca ttttgccaat
    19141 gattattacc ggtcttggcg caacactatt agcgcaattt accggcggga aacctctata
    19201 ctcggcgatt cttgcgcgca cgctggcaaa acaggaagct gagcaactgg cgcgaagcaa
    19261 ggccgcatca gccagcgaga atacttgaac gaaataccag ggtattagat aatggcgatt
    19321 attattgggt tagaatttgc ccgattgccg atgtcgtttg gagcaaaata tgagtgatga
    19381 cgtagcactg ccgctggagt ttaccgacgc agcagccaac aaagttaaaa gcctgatcgc
    19441 tgacgaagat aacccgaatc tgaaattacg cgtgtatatc accggtggcg gttgcagcgg
    19501 cttccagtat ggtttcacct ttgatgatca ggtgaacgaa ggcgatatga ccatcgaaaa
    19561 acagggcgtt ggcctggtgg ttgatccgat gagcctgcaa tatctggtcg gcggttccgt
    19621 tgactacacc gaaggactgg aaggttctcg tttcatcgtg accaacccga acgcgaaaag
    19681 cacctgcggt tgcggttctt cctttagcat ctaatctgtt gttcatgtgc cggataaact
    19741 atccggcaca tcttctctct tcaacgccca ttctcatcca gcgcaaacgt cggtagctta
    19801 agatgccaac gaatagccgc cagccgaatc aatagcgtca cgaccatgcc catcatactg
    19861 gctgtttcca gtggtacgga aaatgtgtaa taagccgtag cgtggacaat accgccgata
    19921 atacaggcag ttgcgtagat ttctgtacgt aaaatcatgg ggatttcgcg ggccagtaca
    19981 tcacgaatga tcccgccgcc aacgccagta atgacgccca tacaaaccgc gattaacgga
    20041 ccggcttccg cattaaaggc tttattcacg ccaatgccga caaacaccgc cagaccaacg
    20101 gcgtccagca ccggcaacat ccattttggt aagcgtcttg gctggcgcac cagcacgatg
    20161 gtcagcatgc tggtgaccat ggcaacgacc agatcggtgg gatctttcac ccaaaatacc
    20221 gggccgtgat ccagcgccat gtcgcgaatt gttccgtcgc ctactgcggt aaccacgccc
    20281 agtaccagaa caccaaaagg gtccatacgc aattttccgg ctaacaaaac gccggagatg
    20341 gcaaataccg ctgtgccgac tatatccagc caatagacga gcattgttca atccccactg
    20401 agcacctgaa aaggtcaggc actaatctac ctgtgaaagc gcattacaga gctgttgtgc
    20461 tgcgaggata atacgtgggc ttgcacgttc aaaccagtca ctcgtgagag gaataacggg
    20521 aattttgagc tgttcacccc agtattgttt gattttagga atttggtccg gtccgcctgt
    20581 aatgacaatc gcctgtggcg agcgtgctaa cacctgttcg cggctaactt gcggccaggg
    20641 aacccggctg tctttaaaga tgttttctcc gccacaaact tcgagtacct ggttctgaat
    20701 cgactctttt ccactggtaa atggcggatt aatgccgaat tgcagaaaaa cacgtttttt
    20761 aggtttatca gcatattgcg ctttcaattg cacgtactga tccagcaggg attgcgcggc
    20821 ttgttcggcc ttgtctggtt gcggactcca gggggccagt tgacgtaacg cattggcaat
    20881 ttgttcaatg cttgtcgcat cgacccacat cacttttatt cccagcgaag ccagctggtc
    20941 aacctgccgc tcggcattac ctccacgcca ggcaatcacc agatcgggtt tcagcgcgac
    21001 aatgcgttcc agattcatcc cctgccaggt ggaaacctgc tcaatctttt gcgcttgtgg
    21061 aggatagtcg gaatagctgc tgaccccaac cggcgtgatc ccggcggcaa aggcaagttc
    21121 agtgttggcg ggagaaagcg tgatgacgcg cggcgcggcg ttgagccaca gtggcgcgag
    21181 aaaagacagg gcgaccagcg ccctgaacag tgatttagcc atgtgccagt ttctgcacca
    21241 gtgactcaac catcaggctg gactgtttag cggcaacagc caggaactca tcgaagctaa
    21301 gatgagactg ttgatcggcc acgtcggaga tggcgcgtac gacaacaaac gggacgttga
    21361 aattgtggca gacatgggcg attgccgtcg cttccatctc tacagcaatg gcctgtggga
    21421 agttgtggcg gattttcgcc agaccaacag aaccgttgat gaaagcgtcg ccgctaacaa
    21481 tcaggccacg tacagcgtta agattcagtt cggcaatgca ggcctcagcg gcagcgatca
    21541 gtttatcgtc agctttaaag cctgtcggac agcctggtaa ctgaccgtat tcataaccaa
    21601 atgccgtgac atccgcgtcg tgataacgcg cttcgtccga gacaacgata tcgcccactt
    21661 tcagcgttgg tgccaggcca ccggcagaac cggtgttaat aatcacatct ggcttgcagt
    21721 gttccaacag cagagtggca cccagcgccg cagcgacttt accgatgccc gatttcagaa
    21781 gcgcaacctc ggttccattc agttggccgg tatagatttc gcaaccgccg agactgatag
    21841 tttgacggtt ttcgattttg tcacgcagca gcgtaacttc ttcttccatt gcaccaatga
    21901 tgccgatttt catagattta ctcgcgataa gcccgatttg aaggcatagt ttaccatgcg
    21961 cttacgggga agcgtatttc tcacgcggga gaggacatgg cacagattga tttccgaaaa
    22021 aaaataaact ggcatcgtcg ttaccgttca ccgcagggcg ttaaaaccga acatgagatc
    22081 ctgcggatct tcgagagcga tcgcgggcgt atcatcaact ctccggcaat tcgtcgtctg
    22141 caacaaaaga cccaggtttt tccactggag cgcaatgccg ccgtgcgcac gcgtcttacc
    22201 cactcgatgg aagtccagca ggtgggacgc tacatcgcca aagaaatttt aagccgtctg
    22261 aaagagctta aattgctgga agcatacggc ctggatgaac tgaccggccc ctttgaaagc
    22321 attgttgaga tgtcatgcct gatgcacgat atcggcaatc cgccgtttgg tcattttggc
    22381 gaagcagcga taaatgactg gtttcgccaa cgtttgcacc cggaagatgc cgaaagccag
    22441 cctctgactg acgatcgctg cagcgtggcg gcactacgtt tacgggacgg ggaagaaccg
    22501 cttaacgagc tgcggcgcaa gattcgtcag gacttatgtc attttgaggg gaatgcacaa
    22561 ggcattcgtc tggtgcatac attgatgcgg atgaatctca cttgggcaca ggttggcggt
    22621 attttaaaat atacccgtcc ggcgtggtgg cgtggcgaaa cgcctgagac acatcactat
    22681 ttaatgaaaa agccgggtta ttatctttct gaagaagcct atattgcccg gttgcgtaaa
    22741 gaacttaatt tggcgcttta cagtcgtttt ccattaacgt ggattatgga agctgccgac
    22801 gacatctcct attgtgtggc agaccttgaa gatgcggtag agaaaagaat atttaccgtt
    22861 gagcagcttt atcatcattt gcacgaagcg tggggccagc atgagaaagg ttcgctcttt
    22921 tcgctggtgg ttgaaaatgc ctgggaaaaa tcacgctcaa atagtttaag ccgcagtacg
    22981 gaagatcagt tttttatgta tttacgggta aacaccctaa acaaactggt accctacgcg
    23041 gcacaacgat ttattgataa tctgcctgcg attttcgccg gaacgtttaa tcatgcatta
    23101 ttggaagatg ccagcgaatg cagcgatctt cttaagctat ataaaaatgt cgctgtaaaa
    23161 catgtattta gccatccgga tgtcgagcag cttgaattgc agggctatcg ggtcattagc
    23221 ggattattag agatttatcg tcctttatta agcctgtcgt tatcagactt tactgaactg
    23281 gtagaaaaag aacgggtgaa acgtttccct attgagtcgc gcttattcca caaactctcg
    23341 acgcgccatc ggctggccta tgtcgaggct gtcagtaaat taccgtcaga ttctcctgag
    23401 tttccgctat gggaatatta ttaccgttgc cgcctgctgc aggattatat cagcggtatg
    23461 accgacctct atgcgtggga tgaataccga cgtctgatgg ccgtagaaca ataaccaggc
    23521 ttttgtaaag acgaacaata aatttttacc ttttgcagaa actttagttc ggaacttcgg
    23581 gctataaaac gaatctgaag aacacagcaa ttttgcgtta tctgttaatc gagactgaaa
    23641 tacatgaaaa aaaccacatt agcactgagt gcactggctc tgagtttagg tttggcgtta
    23701 tctccgctct ctgcaacggc ggctgagact tcttcagcaa cgacagccca gcagatgcca
    23761 agccttgcac cgatgctcga aaaggtgatg ccttcagtgg tcagcattaa cgtagaaggt
    23821 agcacaaccg ttaatacgcc gcgtatgccg cgtaatttcc agcagttctt cggtgatgat
    23881 tctccgttct gccaggaagg ttctccgttc cagagctctc cgttctgcca ggggggccag
    23941 ggcggtaatg gcggcggcca gcaacagaaa ttcatggcgc tgggttccgg cgtcatcatt
    24001 gatgccgata aaggctatgt cgtcaccaac aaccacgttg ttgataacgc gacggtgatt
    24061 aaagtccagc tgagcgatgg tcgtaagttc gacgcgaaga tggttggcaa agatccgcgc
    24121 tctgatatcg cgctgatcca aatccagaac ccgaaaaaac tgaccgcaat taagatggcg
    24181 gattctgatg cactgcgcgt gggtgattac accgtagcga ttggtaaccc gtttggtctg
    24241 ggcgagacgg taacttccgg gattgtctct gcgctggggc gtagcggcct gaatgctgaa
    24301 aactacgaaa acttcatcca gaccgatgca gcgatcaacc gtggtaactc cggtggtgcg
    24361 ctggttaacc tgaacggcga actgatcggt atcaacaccg cgatcctcgc accggacggc
    24421 ggcaacatcg gtatcggttt tgctatcccg agtaacatgg tgaaaaacct gacctcgcag
    24481 atggtggaat acgggcaggt gaaacgcggt gagctgggta ttatggggac tgagctgaat
    24541 tccgaactgg cgaaagcgat gaaagttgac gcccagcgcg gtgctttcgt aagccaggtt
    24601 ctgcctaatt cctcggctgc aaaagcgggc attaaagcgg gtgatgtgat cacctcactg
    24661 aacggtaagc cgatcagcag ctttgccgca ctgcgtgctc aggtgggtac tatgccggtg
    24721 ggcagcaaac tgaccctggg cttactgcgc gacggtaagc aggttaacgt gaacctggaa
    24781 ctgcagcaga gcagccagaa tcaggttgat tccagctcca tcttcaacgg cattgaaggc
    24841 gctgagatga gcaacaaagg taaagatcag ggcgtggtag tgaacaacgt gaagacgggc
    24901 actccggctg cgcagatcgg cctgaagaaa ggtgatgtga ttattggtgc gaaccagcag
    24961 gcagtgaaaa acatcgctga actgcgtaaa gttctcgaca gcaaaccgtc tgtgctggca
    25021 ctgaacattc agcgcggcga cagcaccatc tacctgttaa tgcagtaatc tccctcaacc
    25081 ccttcctgaa tacgggaagg ggttctcctt acaatctgtg aacttcgcca caactccata
    25141 catcttcatc atcctttggg catttgcaca atgccgtacg ttacgtactt ccttatgcta
    25201 agccgtgcat aacggaggac ttatggctgg ctggcatctt gataccaaaa tggcgcagga
    25261 tatcgtggca cgtaccatgc gcatcatcga taccaatatc aacgtaatgg atgcccgcgg
    25321 gcgaattatc ggcagcggcg atcgtgagcg tattggtgaa ttgcacgaag gtgcattgct
    25381 ggtactttca cagggacgag tcgtcgatat cgatgacgcg gtagcacgtc atctgcacgg
    25441 tgtgcggcag gggattaatc taccgttacg gctggaaggt gaaattgtcg gcgtaattgg
    25501 cctgacaggt gaaccagaga atctgcgtaa atatggcgaa ctggtctgca tgacggctga
    25561 aatgatgctg gaacagtcgc ggttgatgca cttgttggcg caggatagcc gtttgcggga
    25621 agaactggtg atgaacctga ttcaggcaga ggagaatact cccgcacttc tgaatgggcg
    25681 caacggctgg ggatcgatct caatcaaccg cgagtggtgg ctattgttga ggtcgacagc
    25741 ggtcagcttg gcgtggacag cgcaatggcg gagttacaac aactgcaaaa cgcgctgact
    25801 acgcccgagc gtaataatct ggtggcgatt gtctcgctaa ccgaaatggt ggtgttgaaa
    25861 ccggcgttga actcttttgg gcgctgggat gcagaagatc atcgtaagcg agttgaacaa
    25921 ctgattaccc gcatgaaaga gtacggccag ctgcgttttc gcgtttcact gggcaactat
    25981 tttaccggtc ctagcagtat tgcccgatcc tatcgtacgg cgaaaacgac gatggtggtg
    26041 ggtaaacagc ggatgccaga aagtcgctgc tatttttatc aggatctgat gttacctgtg
    26101 ttactcgaca gtttgcgtgg cgactggcag gccaacgaac tggcgcgacc gctggcgcgg
    26161 ctgaaagcga tggacaataa cggcttgctg cgacgaacgc tggcggcgtg gtttcgccac
    26221 aatgtgcaac cgctggcaac gtcaaaggcg ttgtttattc atcgtaatac cctggagtat
    26281 cggcttaatc gtatatcgga actgaccggg cttgatttgg gcaattttga tgacaggttg
    26341 ctgctgtatg tggcgttaca actggatgaa gagcggtaga ttatgcgtta aggttgaggc
    26401 ggcgctggct cattcccctc agcccgaagg ggagagggga atgcccgagt taaccgtcaa
    26461 ttcgggatta tttacgcgtt agtttttcca gatcggcttc aatctcgctg atcttattgg
    26521 ttacgactga ctccaggtga cgtaagtcgt cgaggatctt acgcttaaga tcaacttcgc
    26581 tgcggtcacg ctgacagatt tgatcaagct catcaatgat ataccgtaga ttcgggctga
    26641 tttcctggac ttctttataa ccctgaccca caccatcagc gacgaccgtt ttacgctgac
    26701 gcggatattt aaacttaacg ctcttggcga aaaactcgcc tttgtctttc tggaaataga
    26761 ttttcagaat atcgttgttg gcttcctgcc ggaggctgta acgatcaatt tcttcaggat
    26821 tggtaatacc cagacttttc agattgtcgt acatagcgtt acctcaaaat gagtcagtaa
    26881 attgtgctta ttttagcatt tggcttggcc cgcccggctc gactgtgatc gcagaaagcc
    26941 tttttcttcc agacttctat catggcgtaa aaatcaaaaa attacctgct ttattgtggc
    27001 gatattccat gggatggatt agcacattta tcttcgtttt gcgaattaca tgacagtccg
    27061 aaacaagatg aaaaagtatt taaaaaaggg ggaggaagaa tgagtaggac gaattattct
    27121 gagccagaaa gcatacgggc agcatcatgc tgcccgtata tgtactctta atcgatggta
    27181 cgcagcagtt cgttaatgcc gactttgccg cgagttttcg cgtcaacttt cttaacgata
    27241 accgcacagt agaggctgta tttgccatct tttgacggca gattacctga aacaaccaca
    27301 gaccccgccg gaacgcgacc gtagtggatt tcgccggttt cacggtcgta aatacgggtg
    27361 ctctgaccaa tgtatacgcc catagagatg acggaacctt cttcgacaat caccccttca
    27421 accacttcag agcgtgcgcc aatgaagcag ttatcttcaa taatggtcgg gttagcctgc
    27481 agcggctcca gcacgccgcc gatgcccacg ccaccggaaa ggtggacgtt tttaccaatc
    27541 tgcgcacaag aaccgacggt cgcccaggta tcaaccatgg tgccttcatc aacatatgcg
    27601 ccgatgttaa cgtaagacgg catcagcacg gtgttacggg caataaacgc accctgacgt
    27661 accgccgctg gtggcacaac gcggaagcct tctttctgga aacgtgcttc gtcgtagtcg
    27721 gcgaatttca tcggcacttt gtcgaagtag cggctttctg ccccttcgat cacctgatta
    27781 tcattaatac ggaaagagag cagcaccgct tttttcaacc actgatgcgt cacccactga
    27841 ccgtcaattt tttccgctac acgcagtgcg ccggaatcca gcagggcgat cacctgattt
    27901 accgcttcgc gggtaacggt gtctgcattg gctggcgtga tctcggcacg gcgttcaaaa
    27961 gcggtttcaa taatgttctg taactgctgc attgttaaac tcttttcata tcagtaaaca
    28021 catcaccctt tatcgtttgg attgagggcc tctgtcaacc gctgatgcac ttcctgctgc
    28081 aactcgttat taagcgcacg ccggtcagcg gtggcaatta tgaataaatc ttctactcgc
    28141 tcgccaatgg ttgtaattcg ggcaccatga agcgaaattc ccagatcggc aaaaattttc
    28201 ccgactcgcg ccagcagtcc aggttggtcg agggcgatca gttcgaggaa cgatttgcgg
    28261 tcggtatggg tcggcaaaaa cgttacttcg gtttcaacag taaaatggcg taatttggcg
    28321 ggttggcgac ggggctgcgg tggctgccag ctactttgcg tcagcacctg ctccagacca
    28381 aaccgaataa cctcatggcg atctgcggac agcgggctgc catcgggttc cagcacgata
    28441 aaggtatcca tcgccatacc gtcgcgagtg gtgaaaattt gtgcgtcgtg aacacttaaa
    28501 ttgcggcggt ctaattcggc acagacggcg gcaaacagat aggggcggtc cgggctccag
    28561 ataaaaatct cggtgcctcc acgcgtagcc tgcgggctaa gcaataccag cggtttgctt
    28621 aaatcatgct gtaataaatg gcgggcatgc caggccagtt gatttgggct atggcggaca
    28681 aaatagttag cacgacagcg tgaccaaatt tggtgcagcg cctcttcgtc gatgttatcc
    28741 atgcgcagta gtgccagtgc ctggagttgg tgatggcgaa cccgttcgcg catatccggc
    28801 gtgttttgca ttccgcgtcg tagctgcttt tcggtggcaa aatagagttc gcgcaacaga
    28861 ctttgcttcc agctattcca cagcgtttcg ttggtggcgc aaatgtcagc cactgtcagg
    28921 cataccagat agcgcagacg attttccgtt tgaacttctt cggcaaactg cttgatgact
    28981 tcagggtcct gaatatcgcg gcgttgtgct gtcaccgaca tcagcaagtg ctggcgaacc
    29041 agccaggcaa ccagctgtgt ttcgcgtgag ttcagcccgt ggagttctgc aaaatgcact
    29101 acatcctgag caccgagaat ggagtggtcg ccgccgcgtc ctttggcgat atcgtgaaac
    29161 agcgcggcga tgaaaatcag ctcagttgac ggcaggcgcg gccagacgtc cacacacaac
    29221 ggatggcgct ggcgcgtttc ttcactggca aaactctcca gtttcagcat cacgcggata
    29281 gtatgttcat ccaccgtgta ggcgtggaac agatcaaact gcatctgccc gacgatatgc
    29341 gaccattgcg gcatatacgc gccgagcacg ctatggcgat gcattggcaa tagcccgcgc
    29401 cgcaccgctc cggggtgacg cagaatgctc aaaaacagtt ttcgtgcttg cggaatatta
    29461 cacagcggtt gttgcagatg gcgacgggca tggcgtaact ggcgcagcgt ggtggagtaa
    29521 ataccggtga tcgcactgtt gcgcaccatg gtgtagaaca tacgcaagat ggcttccggc
    29581 tggcgcataa atagtgtttc atcacgcagg tcgattagcg taccgcgtag ctgaaactca
    29641 tcgtcgagtg gacgtggttt ttcgtcggcg ggaagggcga ggatggcttc atcgaacagt
    29701 tgcagcagca tctggttgag ttcactgacg cggcgtgtaa cgcggaagta atccttcatc
    29761 atccgctcga ccggttcgtt gccttcacca ctgtaattca gacgctgagc gacgctaagt
    29821 tggcgatcga ataacagacg attatcgtaa cggctgacga ccagatgcag ggcaaagcga
    29881 atacgccaca gtatatgcag acattcgttt aattccgccc gctccgctga ggttaagaag
    29941 ccaaacccga ccatttcatc cagcgatgtt gcgccaaaat gacggcgggc cacccattgc
    30001 agagtgtgaa tatcgcgcaa gccgccaggg ctacttttga tgtctggttc aaggttgtag
    30061 ctggtgccat ggtaacgctg atggcgctgg ttctgttctt caactttcgc tgcgtagaac
    30121 ttgtcggaag gccagaatcc ttcgctaaaa atatgttttt gcagttcgag gaagagcgca
    30181 acatcgccaa ttaataagcg ggattcgatt aaattggtgg cgacggttaa atccgataac
    30241 ccttccaaca tgcactcttc aagcgtgcgc acactatgac cgacttccag ctttacatcc
    30301 cagagcagcg ttaacagctc gcccactttt tgcgcctgat cgtccgggag ctttttacgg
    30361 cttaaaatca gtaaatcgat gtcagaaagt ggatgcagct cgccacgacc gtagccaccg
    30421 acggcaacca atgccaggtc ggcaatctgg ctgaatcccg cttcaatcca taatcgttgc
    30481 aggagctggt ctatgaactc ggtacgtgcc tcaatcaact gttctgcaga gatcccattg
    30541 tcaaaggcat cacccagcca acgctggaaa gtatcgatat gggcttttat cccacagacg
    30601 gttaattcat cacggggcca ggcgcatgga ttttgcggtt gaccgggcag ggtggagaga
    30661 gcggtgtttg cgtactgttc tggaagggta ttcattgtgc gccacccata agattaaatt
    30721 atcgcattaa aaaagccggc attcgccggc ttcatcttat tcgtcgtgcg agattatcgc
    30781 cgggatggtg tcatccttgc gtagcgtcaa aatttcgcag ccgttatcag tcaccacaat
    30841 agtatgctca tattgtgcag acaagctgcg atctttggtt tttaccgtcc agccatcttt
    30901 catggtgcgg atctcttttt tacccgcgtt gaccattggc tcaatggtga acgtcatccc
    30961 cggtttcagc acgacgttgg tttcacggga gtcatagtgc agcacctgcg gttcttcatg
    31021 gaagccgcga ccaataccgt gtccgcaata ttcacgaacg acggagaagc cttctgcttc
    31081 gacaaatttc tgaatcgccg caccgatttc gcgcagatta atgcctggtt ttaccatgcg
    31141 tagcgccagg tacaggcttt cttgcgtgat gcggcacagg cgttcgccca tgatggtcgg
    31201 cttaccgacg ataaacattt tcgaggtatc gccgtggaaa ccatctttga ttacggtgac
    31261 atcaatgtta acgatatcgc catctttcag cagcttagca tcgtccggga taccgtggca
    31321 caccacttca ttaatagaga tgcaaacgga tttcggatag ccgtgatagc cgaggcaggc
    31381 agaaaccgcg tgttgttcat taacaatgta atcattacag atgcgatcca gctcgccggt
    31441 gctgacgccc ggtttaacat acggttcgat catctccagc acttcggcag ccagtcggcc
    31501 agcgacgcgc attttttcga tatcttctgg ggtcttgatt gagatagcca ttaattctgt
    31561 ccatcagcgt cggtgatacc gacaatatat atgtaagtgc cgtcaatggt atcacacccg
    31621 ggcaaattga gaatcattct gaatttcgcc aaacgtgcca ctgaaggttt tctataatag
    31681 aaaattcgac gtctgatgct gtacacagcg ccaacaatta ttggtgtcca cgacgtattt
    31741 gtggtataaa gcgcgccgga cttccgatcc atttcgtata cacagactgg acggaagcga
    31801 caatctcact ttgtgtaaca acacacacgt atcggcacat attccggggt gccctttggg
    31861 gtcggtaata tgggatacgt ggaggcataa ccccaacttt tatatagagg ttttaatcat
    31921 ggcaactgtt tccatgcgcg acatgctcaa ggctggtgtt cacttcggtc accagacccg
    31981 ttactggaac ccgaaaatga agccgttcat cttcggtgcg cgtaacaaag ttcacatcat
    32041 caaccttgag aaaactgtac cgatgttcaa cgaagctctg gctgaactga acaagattgt
    32101 ttctcgcaaa ggtaaaatcc ttttcgttgg tactaaacgc gctgcaagcg aagcggtgaa
    32161 agacgctgct ctgagctgcg accagttctt cgtgaaccat cgctggctgg gcggtatgct
    32221 gactaactgg aaaaccgttc gtcagtccat caaacgtctg aaagacctgg aaactcagtc
    32281 tcaggacggt actttcgaca agctgaccaa gaaagaagcg ctgatgcgca ctcgtgagct
    32341 ggagaaactg gaaaacagcc tgggcggtat caaagacatg ggcggtctgc cggacgctct
    32401 gtttgtaatc gatgctgacc acgaacacat tgctatcaaa gaagcaaaca acttgggtat
    32461 tccggtattt gctatcgttg ataccaactc tgatccggac ggtgttgact tcgttatccc
    32521 gggtaacgac gacgcaatcc gtgctgtgac cctgtacctg ggcgctgttg ctgcaaccgt
    32581 acgtgaaggc cgttctcagg atctggcttc ccaggcggaa gaaagcttcg tagaagctga
    32641 gtaataaggc ttgataactc ccccaaaata gttcgagttg cagaaaggcg gcaagctcga
    32701 gaattcccgg gagcttacat cagtaagtga ccgggatgag cgagcgaaga caacgcatct
    32761 gcggcgcgaa atatgaaggg ggagagccct tatagaccag gtagtacacg tttggttagg
    32821 gggcctgcat atggccccct ttttcacttt tatatctgtg cggtttaatg ccgggcagat
    32881 cacatctccg aggattttag aatggctgaa attaccgcat ccctggtaaa agagctgcgt
    32941 gagcgtactg gcgcaggcat gatggattgc aaaaaagcac tgactgaagc taacggcgac
    33001 atcgagctgg caatcgaaaa catgcgtaag tccggtgcta ttaaagcagc gaaaaaagca
    33061 ggcaacgttg ctgctgacgg cgtgatcaaa accaaaatcg acggcaacta cggcatcatt
    33121 ctggaagtta actgccagac tgacttcgtt gcaaaagacg ctggtttcca ggcgttcgca
    33181 gacaaagttc tggacgcagc tgttgctggc aaaatcactg acgttgaagt tctgaaagca
    33241 cagttcgaag aagaacgtgt tgcgctggta gcgaaaattg gtgaaaacat caacattcgc
    33301 cgcgttgctg cgctggaagg cgacgttctg ggttcttatc agcacggtgc gcgtatcggc
    33361 gttctggttg ctgctaaagg cgctgacgaa gagctggtta aacacatcgc tatgcacgtt
    33421 gctgcaagca agccagaatt catcaaacca gaagacgtat ccgctgaagt ggtagaaaaa
    33481 gaataccagg tacagctgga tatcgcgatg cagtctggta agccgaaaga aatcgcagag
    33541 aaaatggttg aaggccgcat gaagaaattc accggcgaag tttctctgac cggtcagccg
    33601 ttcgttatgg aaccaagcaa aactgttggt cagctgctga aagagcataa cgctgaagtg
    33661 actggcttca tccgtttcga agtgggtgaa ggcatcgaga aagttgagac tgactttgca
    33721 gcagaagttg ctgcgatgtc caagcagtct taattatcaa aaaggagccg cctgagggcg
    33781 gcttcttttt gtgcccatct tgtaaattca gctaaccctt gtggggctgc gctgaaaagc
    33841 gacgtacaat gtcgctagta ttaattcatt tcaatcgttg acagtctcag gaaagaaaca
    33901 tggctaccaa tgcaaaaccc gtctataaac gcattctgct taagttgagt ggcgaagctc
    33961 tgcagggcac tgaaggcttc ggtattgatg caagcatact ggatcgtatg gctcaggaaa
    34021 tcaaagaact ggttgaactg ggtattcagg ttggtgtggt gattggtggg ggtaacctgt
    34081 tccgtggcgc tggtctggcg aaagcgggta tgaaccgcgt tgtgggcgac cacatgggga
    34141 tgctggcgac cgtaatgaac ggcttggcaa tgcgtgatgc actgcaccgc gcctatgtga
    34201 acgctcgtct gatgtccgct attccattga atggcgtgtg cgacagctac agctgggcag
    34261 aagctatcag cctgttgcgc aacaaccgtg tggtgatcct ctccgccggt acaggtaacc
    34321 cgttctttac caccgactca gcagcttgcc tgcgtggtat cgaaattgaa gccgatgtgg
    34381 tgctgaaagc aaccaaagtt gacggcgtgt ttaccgctga tccggcgaaa gatccaaccg
    34441 caaccatgta cgagcaattg acttacagcg aagtgctgga aaaagagctg aaagtcatgg
    34501 acctggcggc cttcacgctg gctcgtgacc ataaattacc gattcgtgtt ttcaatatga
    34561 acaaaccggg tgcgctgcgc cgtgtggtaa tgggtgaaaa agaagggact ttaatcacgg
    34621 aataattccc gtgatggata aataagggta agattccgcg taagtatcgc gggggcgtaa
    34681 gtctggttat aaggcgttat tgttgcaggc agtttggtca cggccagcgc gcagcaaccg
    34741 gagcgtacaa aagtacgtga ggatggcgag cactgctcgg ggccaaaatg gcaaataaaa
    34801 tagcctaata atccagacga ttacccgtaa tatgtttaat cagggctata cttagcacac
    34861 ttccactgtg tgtgactgtc tggtctgact gagacaagtt ttcaaggatt cgtaacgtga
    34921 ttagcgatat cagaaaagat gctgaagtac gcatggacaa atgcgtagaa gcgttcaaaa
    34981 cccaaatcag caaaatacgc acgggtcgtg cttctcccag cctgctggat ggcattgtcg
    35041 tggaatatta cggcacgccg acgccgctgc gtcagctggc aagcgtaacg gtagaagatt
    35101 cccgtactct gaaaatcaac gtgtttgatc gttcaatgtc tccggccgtt gaaaaagcga
    35161 ttatggcctc cgatctcggc ctgaacccga actctgcggg tagcgacatc cgtgttccgc
    35221 tgccgccgct gacggaagaa cgtcgtaaag atctgaccaa aatcgttcgt ggtgaagcag
    35281 aacaagcgcg tgttgcagta cgtaatgtgc gtcgtgacgc gaacgacaaa gtgaaagcac
    35341 tgttgaaaga taaagagatc agcgaagacg acgatcgccg ttctcaggac gatgtacaga
    35401 aactgactga tgctgcaatc aagaaaattg aagcggcgct ggcagacaaa gaagcagaac
    35461 tgatgcagtt ctgatttctt gaacgacaaa aacgccgctc agtagatcct tgcggatcgg
    35521 ctggcggcgt tttgcttttt attctgtctc aactctggat gtttcatgaa gcaactcacc
    35581 attctgggct cgaccggctc gattggttgc agcacgctgg acgtggtgcg ccataatccc
    35641 gaacacttcc gcgtagttgc gctggtggca ggcaaaaatg tcactcgcat ggtagaacag
    35701 tgcctggaat tctctccccg ctatgccgta atggacgatg aagcgagtgc gaaacttctt
    35761 aaaacgatgc tacagcaaca ggggagccgc accgaagtct taagtgggca acaagccgct
    35821 tgcgatatgg cagcgcttga ggaggttgat caggtgatgg cagccattgt tggtgctgct
    35881 gggctgttac ctacgcttgc tgcgatccgc gcgggtaaaa ccattttgct ggccaataaa
    35941 gaatcactgg ttacctgcgg acgtctgttt atggacgccg taaagcagag caaagcgcaa
    36001 ttgttaccgg tcgatagcga acataacgct atttttcaga gtttaccgca acctatccag
    36061 cataatctgg gatacgctga ccttgagcaa aatggcgtgg tgtccatttt acttaccggg
    36121 tctggtggcc ctttccgtga gacgccattg cgcgatttgg caacaatgac gcccgatcaa
    36181 gcttgccgtc atccgaactg gtcgatggga cgtaaaattt ctgtcgattc ggctaccatg
    36241 atgaacaaag gtctggaata cattgaagcg cgttggctgt ttaacgccag cgccagccag
    36301 atggaagtgc tgattcaccc gcagtcagtg attcactcaa tggtgcgcta tcaggacggc
    36361 agtgttctgg cgcagctggg ggaaccggat atgcgtacgc caattgccca caccatggca
    36421 tggccgaatc gcgtgaactc tggcgtgaag ccgctcgatt tttgcaaact aagtgcgttg
    36481 acatttgccg caccggatta tgagcgttat ccatgcctga aactggcgat ggaggcgttc
    36541 gaacaaggcc aggcagcgac gacagcattg aatgccgcaa acgaaatcac cgttgctgct
    36601 tttcttgcgc aacaaatccg ctttacggat atcgctgcgt tgaatttatc tgtactggaa
    36661 aaaatggata tgcgcgaacc acaatgtgtg gacgatgtgt tatctgttga tgcgaacgcg
    36721 agtgaagtcg ccagaaaaga ggtgatgcgt ctcgcaagct gaggataatc cggctacaga
    36781 gagtcgcgct atttgttagc gtagggcttc aatgatatag tctgcgccat ctgatcgtaa
    36841 gtagttggct ttataaggtc aggtatgccg tggttttaca cggctttttt ttgtataggc
    36901 ttcagtattc ctgagtaccg taaaccctgt cagggaataa aaaacgcgtg atgttgtctg
    36961 ctactcaacc acttagcgaa aaattgccag cgcatggctg ccgtcatgtt gcgatcatta
    37021 tggacggcaa tggtcgctgg gcaaaaaagc aagggaagat tcgtgccttt gggcataaag
    37081 ccggggcaaa atccgtccgc cgggctgtct cttttgcggc caacaacggt attgaagcgt
    37141 taacgctgta tgcctttagt agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt
    37201 taatggaact gtttgtgtgg gcgctcgata gcgaagtaaa aagtctgcac cgacataacg
    37261 tgcgtctgcg tattattggc gataccagtc gttttaactc gcgtttgcaa gaacgtattc
    37321 gtaaatctga agcgctaaca gccggaaata ccggtctgac gctgaatatt gcggcgaact
    37381 acggtggacg ttgggatata gtccagggag tcaggcaact ggctgaaaag gtgcagcaag
    37441 gaaacctgca accagatcag atagatgaag agatgttaaa ccagcatgtc tgtatgcatg
    37501 aactggcccc tgtagattta gtaattagga ctgggggaga gcatcgcatt agtaactttt
    37561 tgctttggca aattgcctat gccgaacttt actttacaga tgttctctgg cccgatttcg
    37621 atgaacaaga ctttgaaggg gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg
    37681 gcaccgagcc cggtgatgaa acagcctgat gggggtcgct tttgctgaag tatcgcctga
    37741 tatctgcttt tgtgttaata cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg
    37801 ggttcgccat tgtaacgctg gtggtctgca tgctggcagc gtgggaatgg ggacagctta
    37861 gcggttttac cactcgttcg cagcgagtat ggttggcggt gttatgcggg ttattgttgg
    37921 cgctgatgct ttttctgttg ccggaatatc accgaaatat tcatcaaccg ctggttgaaa
    37981 tctcactttg ggcttcgttg ggttggtgga ttgtcgcgct attgctggtg ctgttttacc
    38041 caggttccgc agcaatctgg cgtaactcta aaacattgcg ccttattttt ggcgtgctaa
    38101 ccattgttcc cttcttctgg ggcatgctgg cgttacgggc ctggcactat gacgagaatc
    38161 attacagtgg cgcaatatgg ctgctctatg tcatgatcct ggtatggtgc gctgactccg
    38221 gcgcatatat gtttggcaaa ttgtttggta aacataagct ggcaccgaag gtttctccgg
    38281 gtaaaacctg gcaaggcttt ataggtggac tcgctactgc agcggtaatc tcatggggtt
    38341 atggcatgtg ggcgaatctc gacgtcgctc ccgtcacctt actcatttgc tctattgtcg
    38401 cagcgttagc ctcagtgctc ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa
    38461 ttaaggacag cggtcattta attccaggac acggtggtat tttagatcgt attgatagcc
    38521 tgacggctgc ggtaccggtc tttgcttgct tgttgttact ggtattcagg acgctttaac
    38581 ggaaggtaat atgctgagtt ttctctggga tttggcttcg ttcatcgttg cactgggtgt
    38641 acttatcacc gtgcatgaat ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt
    38701 tgagcgtttc tcaatagggt ttggtaaggc gctctggcgg cgaactgata agctcggcac
    38761 cgaatatgtt atcgccctga tcccgttggg cggttatgtc aaaatgctgg atgagcgcgc
    38821 agaaccggtc gttccggaac tccgccacca tgccttcaat aataaatctg tcggccaacg
    38881 agtggcgatt attgccgcag gtccggttgc aaacttcatt tttgctatct ttgcctactg
    38941 gctggttttt attattggtg tgcctggcgt acgtccggtg gttggcgaaa tagcagccaa
    39001 ttcgatagct gcggaagcac aaattgcacc aggtacggaa ctaaaagccg tagatggtat
    39061 cgaaacgcct gattgggatg ccgtgcgttt gcagttggtc gataaaattg gcgatgaaag
    39121 caccaccatt acagtagcgc catttggcag cgaccaacgg cgggatgtaa agctcgattt
    39181 acgtcactgg gcgtttgagc ctgataaaga agatccggta tcttcgctgg ggattcgtcc
    39241 tcgtgggccg caaattgaac ctgtactgga aaatgtgcag ccaaactcgg cggcaagcaa
    39301 ggcaggtttg caagcaggcg acaggatcgt taaagtcgat ggtcagccct taacgcagtg
    39361 ggtgaccttt gtgatgcttg tccgggataa cccgggtaaa tccttagcgt tagaaatcga
    39421 aaggcagggg agtcccttgt ctttgacatt aatcccggag agtaaaccgg gtaatggtaa
    39481 agcgattggt tttgtcggta ttgagccgaa agtcattcct ttgccagatg agtataaagt
    39541 tgtacgccag tatgggccgt tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct
    39601 gatgaagctg acggtcagta tgctgggaaa attgatcacc ggtgatgtga aactgaacaa
    39661 cctcagtggg ccgatttcta tcgccaaggg ggctgggatg acagcggaac tcggggtagt
    39721 ttattacctg ccgtttcttg cgcttattag cgtgaactta gggataatta acctgtttcc
    39781 gttgcccgta cttgacgggg ggcatctgct gttccttgcg atcgaaaaga tcaagggcgg
    39841 accggtatcc gagcgggttc aagacttttg ttatcgcatt ggctcgattc tgctggtgct
    39901 gttaatgggg cttgcacttt tcaatgattt ctctcggtta tgagagttag ttaggaagaa
    39961 cgcataataa cgatggcgat gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc
    40021 accgtatacg gtgctgaagg gttcgtagtg aaagatattc atttcgaagg ccttcagcgt
    40081 gtcgccgttg gtgcggccct cctcagtatg ccggtgcgta caggcgacac ggttaatgat
    40141 gaagatatca gtaataccat tcgcgctctg tttgctaccg gcaactttga ggatgttcgc
    40201 gtccttcgtg atggtgatac ccttctggtt caggtaaaag aacgtccgac cattgccagc
    40261 attactttct ccggtaacaa atcggtgaaa gatgacatgc taaagcaaaa cctcgaggct
    40321 tctggtgtgc gtgtgggcga atccctcgat cgcaccacca ttgccgatat cgagaaaggt
    40381 ctggaagact tctactacag cgtcggtaaa tatagcgcca gcgttaaagc tgtcgtgacc
    40441 ccgctgccgc gcaaccgtgt tgacctaaaa ctggtgttcc gggaaggtgt gtcagctgaa
    40501 atccagcaaa ttaacattgt tggtaaccat gctttcacca ccgacgaact gatctctcat
    40561 ttccaactgc gtgacgaagt gccgtggtgg aatgtggtag gcgatcgtaa ataccagaaa
    40621 cagaaactgg cgggcgacct tgaaaccctg cgcagctact atctggaccg cggttatgcc
    40681 cgtttcaaca tcgactctac ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc
    40741 acggtgaaca tcaccgaagg cgatcagtac aagctttctg gcgttgaagt gagcggcaac
    40801 cttgccgggc actccgctga aattgagcag ctgactaaga tcgagccggg cgagctgtat
    40861 aacggcacta aagtgaccaa aatggaagat gacattaaaa agcttctcgg tcgctatggt
    40921 tatgcctatc cgcgcgtaca gtcgatgcct gaaattaacg atgccgacaa aaccgttaaa
    40981 ttacgcgtga acgttgatgc gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt
    41041 aacgatacct cgaaagatgc cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg
    41101 ttggggagcg atctggtcga tcagggtaag gagcgtctga atcgtctggg cttctttgaa
    41161 actgtcgata ctgataccca acgtgttccg ggtagcccgg accaggttga tgtagtctac
    41221 aaggtgaaag aacgtaacac tggtagcttc aacttcggga ttggttacgg tactgaaagt
    41281 ggcgtgagct tccaggctgg cgtgcaacag gataactggt taggtacagg ttatgctgtt
    41341 ggtatcaacg ggaccaaaaa cgattaccag acctatgctg aactgtcggt aaccaacccg
    41401 tacttcaccg tagatggcgt aagcctcggt ggtcgtctct tctataatga cttccaggca
    41461 gatgacgccg acctgtccga ctataccaac aagagttatg gtacagacgt gacgttgggc
    41521 ttcccgatta acgaatataa ctcgctgcgt gcaggtctgg gttatgtaca taactccctg
    41581 tccaacatgc agcctcaggt tgcgatgtgg cgttatctgt actctatggg tgaacatccg
    41641 agcacttctg atcaggataa cagcttcaaa acggacgact tcacgttcaa ctatggttgg
    41701 acctataaca agcttgaccg tggttacttc ccgacagatg gttcacgtgt caacctgacc
    41761 ggtaaagtga ccattcctgg atcggataac gaatactaca aagtgacgtt agacacggcg
    41821 acttatgtgc cgatcgatga cgatcacaaa tgggttgttc tgggtcgtac ccgctggggt
    41881 tatggtgatg gtttaggcgg caaagagatg ccgttctacg agaacttcta tgccggtggt
    41941 tccagcaccg tgcgtggctt ccagtccaat accattggtc cgaaagcagt ttacttcccg
    42001 catcaggcca gtaattatga tccggactat gattacgaat gtgcgactca ggacggcgcg
    42061 aaagacctgt gtaaatcgga tgatgctgta ggcggtaacg ccatggcggt tgccagcctc
    42121 gagttcatca ccccgacgcc gtttattagc gataagtatg ctaactcggt tcgtacttca
    42181 ttcttctggg atatgggtac cgtttgggat acaaactggg attccagcca atattctgga
    42241 tatccggact atagtgatcc aagcaatatc cgtatgtctg cgggtatcgc attacaatgg
    42301 atgtccccat tggggccgtt ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga
    42361 gacaaggcag aacagttcca gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag
    42421 gaatgtagtg gtagtgtagc gatgacttta ggcgatcaat ataagatcgc cgggccacgc
    42481 aaagaactgc accctccggt gcaaatggga tggtaaggag tttattgtga aaaagtggtt
    42541 attagctgca ggtcttggtt tagcactggc aacttctgct caggcggctg acaaaattgc
    42601 aatcgtcaat atgggcagcc tgttccagca ggtagcgcag aaaaccggtg tttctaacac
    42661 gctggaaaat gagttcaaag gccgtgccag cgaactgcag cgtatggaaa ccgatctgca
    42721 ggctaaaatg aaaaaactgc agtccatgaa agcgggcagc gatcgcacca aactggaaaa
    42781 agacgtgatg gctcagcgcc agacttttgc tcagaaagcg caggcttttg agcaggatcg
    42841 cgcacgtcgt tccaacgaag aacgcggcaa actggttact cgtatccaga ctgctgtgaa
    42901 atccgttgcc aacagccagg atatcgatct ggttgttgat gcaaacgctg ttgcttacaa
    42961 cagcagcgat gtaaaagaca tcactgccga cgtactgaaa caggttaaat aagtaatgcc
    43021 ttcaattcga ctggctgatt tagcgcagca gttggatgca gaactacacg gtgatggcga
    43081 tatcgtcatc accggcgttg cgtccatgca atctgcacaa acaggtcaca ttacgttcat
    43141 ggttaaccca aaataccgtg agcatttagg cttgtgccaa gcgtccgcgg ttgtcatgac
    43201 ccaggacgat cttcctttcg cgaaaagtgc cgcgctggta gtgaagaatc cctacctgac
    43261 ttacgcgcgc atggcgcaaa ttttagatac cacgccgcag cccgcgcaga acattgcacc
    43321 cagtgcggtg atcgacgcga cggcgaagct gggtaacaac gtatcgattg gcgctaacgc
    43381 ggtgattgag tccggcgttg aactgggcga taacgtgatt atcggtgccg gttgcttcgt
    43441 aggtaaaaac agcaaaatcg gtgcaggttc gcgtctctgg gcgaacgtaa ccatttacca
    43501 tgagatccag atcggtcaga attgcctgat ccagtccgga acagtggtag gcgcagacgg
    43561 ctttggttat gccaacgatc gtggtaactg ggtgaagatc ccacagattg gtcgcgtaat
    43621 tattggcgat cgagtggaga tcggtgcctg cacaaccatc gatcgcggcg cgctggatga
    43681 cactgttatt ggcaatggcg tgatcattga taaccagtgc cagattgcac ataacgtcgt
    43741 gattggcgac aatacggcgg ttgccggtgg cgtcattatg gcgggcagcc tgaaaattgg
    43801 tcgttactgc atgatcggcg gagccagcgt aatcaacggg catatggaaa tatgcgacaa
    43861 agtgacggtt acgggcatgg gtatggtgat gcgtcccatc actgaaccag gcgtctattc
    43921 ctcaggcatt ccgctgcaac ccaacaaagt ctggcgcaaa accgctgcac tggtgatgaa
    43981 cattgatgac atgagcaagc gtctgaaatc gcttgagcgc aaggttaatc aacaagacta
    44041 acgttccatc ttttgttcgc caaactttac ggcctgtctc attcttacga ttgcggcagg
    44101 ccgtgttatt attgtcgttt cttatatttt gacaggaaga gtatcttgac tactaacact
    44161 catactctgc agattgaaga gattttagaa cttctgccgc accgtttccc gttcttactg
    44221 gtggatcgcg tgctggattt tgaagaaggt cgttttctgc gcgcagtaaa aaatgtctct
    44281 gtcaatgagc cattcttcca gggccatttc cctggaaaac cgattttccc gggtgtgctg
    44341 attctggaag caatggcaca ggcaacaggt attctggcgt ttaaaagcgt aggaaaactg
    44401 gaaccgggtg agctgtacta cttcgctggt attgacgaag cgcgcttcaa gcgcccggtc
    44461 gtgcctggcg atcaaatgat catggaagtc actttcgaaa aaacgcgccg cggcctgacc
    44521 cgttttaaag gggttgctct ggtcgatggt aaagtagttt gcgaagcaac gatgatgtgt
    44581 gctcgtagcc gggaggcctg atacgtgatt gataaatccg cctttgtgca tccaaccgcc
    44641 attgtggaag agggcgcgtc aattggcgcg aacgcacaca ttggtccttt ttgtatcgtt
    44701 ggaccccatg tcgaaattgg tgagggtacc gtactgaaat ctcacgttgt cgtgaatggt
    44761 catactaaaa ttggccgcga taatgagatt tatcagtttg cctccatcgg cgaagttaac
    44821 caggatttga aatatgctgg cgaaccgacc cgtgtggaaa tcggcgatcg taaccgcatt
    44881 cgcgaaagcg tcaccattca tcgtggcaca gtccagggcg gtggattgac gaaggtgggc
    44941 agcgacaact tactgatgat caacgcgcac attgcgcacg attgtacggt aggtaaccgc
    45001 tgtattctcg ccaacaacgc aacgctggcg ggtcacgtat cggttgacga cttcgcgatc
    45061 atcggcggca tgaccgcagt ccatcagttc tgcatcattg gtgcgcatgt gatggttggc
    45121 ggctgctccg gtgtggcgca ggacgtccct ccttatgtca ttgcgcaggg taaccacgca
    45181 acgccgttcg gtgtcaatat cgaagggctg aagcgccgcg gattcagccg tgaggcgatt
    45241 accgctatcc gcaatgcgta taagctaatt tatcgtagcg gtaaaacgct cgatgaagtg
    45301 aaaccggaaa ttgctgaact ggcggaaaca tatccggaag tgaaagcctt taccgatttc
    45361 tttgcacgct caacgcgcgg tctgattcgt taatgactga ccagcgtcca ttaacgattg
    45421 ccctggtcgc cggagaaatc tccggcgata tcctgggggc cggtttaatc cgcgctctga
    45481 aagaacgtgt gcccaacgcc cgctttgttg gtgttgccgg gccacgaatg caggctgaag
    45541 gctgcgaagc ctggtacgaa atggaagaac tggcggtgat gggcattgtt gaagtgctcg
    45601 gtcgtctgcg tcgcttactg catattcgtg ccgatctgac aaagcgtttt ggcgaactga
    45661 agccagatgt ttttgttggt attgatgcgc ccgacttcaa tattactctt gaaggtaacc
    45721 tcaaaaagca gggtatcaaa accattcatt acgtcagtcc atccgtctgg gcgtggcgac
    45781 agaaacgcgt tttcaaaata ggcagagcca ccgatctggt gctcgcattt ctgcctttcg
    45841 aaaaagcgtt ttatgacaaa tacaatgtac cgtgccgctt tatcggtcat accatggctg
    45901 atgccatgcc gttagatcca gataaaaatg gtgcccgtga tgtgctgggg atcccttacg
    45961 atgcccactg tctggcattg ttgccgggca gccgtggtgc agaagtcgaa atgcttagtg
    46021 ccgatttctt gaaaactgcc cagcttttgc gccagacata tccggatctc gaaatcgtgg
    46081 tgccgctggt gaatgccaaa cgccgcgagc agtttgaacg catcaaagct gaagtcgcgc
    46141 cagacctgtc ggttcatttg ctggatggaa tgggccgtga ggcgatggtc gccagcgatg
    46201 cggcactact ggcgtcgggg acggcagccc tggagtgtat gctggcgaaa tgcccgatgg
    46261 tggtgggata tcgcatgaag ccttttacct tctggttggc gaagcggctg gtgaaaactg
    46321 attatgtctc gctgccaaat ctgctggcgg gcagagagtt agtcaaagag ttattgcagg
    46381 aagagtgtga gccgcaaaaa ctggctgcgg cgctgttacc gctgttggcg aacgggaaaa
    46441 ccagccacgc gatgcacgat accttccgtg aactgcatca gcagatccgc tgcaatgccg
    46501 atgagcaggc ggcacaagcc gttctggagt tagcacaatg atcgaatttg tttatccgca
    46561 cacgcagctg gttgcgggtg tggatgaagt cggacgcggg ccgttagttg gcgcggtcgt
    46621 caccgctgcg gtgatccttg acccggcgcg cccgattgcc gggctgaatg attccaaaaa
    46681 gctgagcgaa aaacgccgtc tggcgctcta tgaagagatc aaagagaaag cgttgagctg
    46741 gagtctgggc cgcgcggaac cccacgaaat cgacgagctg aacattcttc atgcgaccat
    46801 gctggcgatg cagcgtgccg tcgctgggct gcatattgcg ccggaatatg tgttgattga
    46861 tggtaaccgc tgcccgaaat taccgatgcc tgcgatggct gtggtgaaag gggatagccg
    46921 cgtaccagaa atcagtgccg cgtctatcct agcgaaagtg acgcgtgacg ccgaaatggc
    46981 ggcgctggat attgttttcc cgcaatatgg ttttgcccaa cataaagggt atccaaccgc
    47041 ttttcatctg gaaaaactgg ctgaatatgg cgcgactgaa catcatcgtc gcagctttgg
    47101 gcctgtcaaa cgcgcactgg gacttgcgtc ctgattcttg tgtcgagatt aagtaaaccg
    47161 gaatctgaag atgtctgaac cacgtttcgt acacctgcgg gtgcacagcg actactcgat
    47221 gatcgatggc ttggccaaaa ccgcaccgct ggtaaaaaag gcggcggcgt tgggtatgcc
    47281 agcactggcg atcaccgatt tcaccaacct ttgcggtctg gtgaagttct acggagcggg
    47341 acatggcgca gggattaagc ctatcgtcgg ggcagatttt aatgtccagt gcgacctgct
    47401 gggtgatgag ttaacccacc tgacggtact ggcggcgaac aataccggct atcagaatct
    47461 ggcgttgctg atctcaaaag cgtatcagcg cgggtacggt gccgccgggc cgatcatcga
    47521 tcgcgactgg cttatcgaat taaacgaagg gttgatcctt ctttccggcg ggcgcatggg
    47581 cgacgtcgga cgcagtcttt tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt
    47641 ttatgaagaa cacttcccgg atcgctattt tctagagctg atccgcaccg gcaggccgga
    47701 tgaagaaagc tatctgcacg cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt
    47761 ggcgactaac gatgtgcgct ttatcgacag cagcgacttt gacgcacacg aaatccgcgt
    47821 cgcgatccac gacggcttta ccctcgacga tcctaaacgc ccgcgtaact attcgccgca
    47881 gcaatatatg cgtagcgaag aggagatgtg tgagctgttt gccgacatcc cagaagccct
    47941 tgccaacacc gttgagatcg ccaaacgctg taacgtaact gtgcgtcttg gtgaatactt
    48001 cctgccgcag ttcccgaccg gggacatgag caccgaagat tatctggtca agcgtgcaaa
    48061 agaaggcctg gaagagcgtc tggcattttt attccctgac gaggaagaac gtcttaaacg
    48121 ccgcccggaa tatgacgaac gtctggagac tgaacttcag gttatcaacc agatgggttt
    48181 cccgggctac ttcctcatcg ttatggaatt tatccagtgg tcgaaagata acggcgtacc
    48241 ggtaggacca ggccgtggct ccggtgcggg ttcactggtg gcctacgcgc tgaaaatcac
    48301 cgacctcgat ccgctggaat ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt
    48361 ctccatgcct gacttcgacg ttgacttctg tatggagaaa cgcgatcagg ttatcgaaca
    48421 cgtggcggac atgtacggtc gtgatgcggt atcgcagatt atcaccttcg gtacgatggc
    48481 ggcgaaagcg gtgatccgcg acgtaggccg cgtgctgggg catccgtacg gctttgtcga
    48541 ccgtatctcg aaactgatcc cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc
    48601 cgagccgcag ctgccggaaa tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat
    48661 ggcgcgcaaa ctggaagggg tcacccgtaa cgccggtaag cacgccggtg gggtggttat
    48721 cgcgccgacc aaaattaccg attttgcgcc gctttattgc gatgaagagg gcaaacatcc
    48781 ggtcacccag tttgataaaa gcgacgttga atacgccgga ctggtgaagt tcgacttcct
    48841 tggtttgcgt acgctcacca tcatcaactg ggcgctggag atgatcaaca agcggcgggc
    48901 gaagaatggc gagccaccgc tggatatcgc cgcgatcccg ctggatgaca agaaaagctt
    48961 cgacatgctg caacgctcgg aaaccacggc ggtattccag cttgaatcgc gcggcatgaa
    49021 ggacctgatc aagcgtctgc aacctgactg cttcgaagat atgatcgcac tggtggcact
    49081 gttccgccct ggtccgttgc agtcagggat ggtggataac tttatcgacc gtaaacatgg
    49141 tcgtgaagag atctcctatc cggacgtgca gtggcagcat gaaagcctga aatcggtact
    49201 ggagccaacc tacggcatta tcttgtatca ggaacaggtc atgcagattg cccaggtgct
    49261 ttctggttat accctcggtg gcgcggatat gctgcgccgt gcgatgggta agaaaaagcc
    49321 ggaagagatg gctaagcagc gttctgtatt tgctgaaggt gcagaaaaga acggaatcaa
    49381 cgccgaactg gcgatgaaaa tcttcgacct ggtggagaaa ttcgcgggtt acggatttaa
    49441 caaatcgcac tctgcggcct atgctttggt gtcatatcaa acgttatggc tgaaagcgca
    49501 ctatccggcg gagtttatgg cggcggtaat gaccgccgat atggacaaca ccgagaaggt
    49561 ggtgggcctg gtggatgagt gctggcggat ggggctgaaa atcctgccac cagatataaa
    49621 ctccggtctt taccatttcc acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc
    49681 catcaaaggg gtaggtgaag gtccgattga ggccatcatc gaagcccgta ataaaggcgg
    49741 ctacttccgc gaactgtttg atctctgcgc ccgtaccgac accaaaaagt taaaccggcg
    49801 ggtgctggaa aaactgatca tgtccggggc gtttgaccgt cttgggccac accgcgcggc
    49861 gctgatgaac tcgctgggtg atgcgttaaa agcggcagat caacacgcga aagcggaagc
    49921 tatcggtcag gccgatatgt tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc
    49981 ctacgccagc tgccaaccgt ggccggagca ggtggtatta gatggggaac gtgaaacgtt
    50041 aggcctgtac ctgaccggac accctatcaa ccagtattta aaagagattg agcgttatgt
    50101 cggaggcgta aggctgaaag acatgcaccc gacagaacgt ggtaaagtca tcacagctgc
    50161 ggggctcgtt gttgccgcgc gggttatggt caccaagcgc ggcaatcgta tcggtatctg
    50221 cacgctggat gaccgttccg ggcggctgga agtgatgttg tttactgacg ccctggataa
    50281 ataccagcaa ttgctggaaa aagaccgcat acttatcgtc agcggacagg tcagctttga
    50341 tgacttcagc ggtgggctta aaatgaccgc tcgcgaagtg atggatattg acgaagcccg
    50401 ggaaaaatat gctcgcgggc ttgctatctc gctgacggac aggcaaattg atgaccagct
    50461 tttaaaccga ctccgtcagt ctctggaacc ccaccgctct gggacaattc cagtacatct
    50521 ctactatcag agggcggatg cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc
    50581 tccgagcgat cgtttattaa acgatctccg tggcctcatt ggttcggagc aggtggaact
    50641 ggagtttgac taatacagga atactatgag tctgaatttc cttgattttg aacagccgat
    50701 tgcagagctg gaagcgaaaa tcgattctct gactgcggtt agccgtcagg atgagaaact
    50761 ggatattaac atcgatgaag aagtgcatcg tctgcgtgaa aaaagcgtag aactgacgcg
    50821 taaaatcttc gccgatctcg gtgcatggca gattgcgcaa ctggcacgcc atccgcagcg
    50881 tccttatacc ctagattacg ttcgcctggc atttgatgaa tttgacgaac tggctggcga
    50941 ccgtgcgtat gcagacgata aagctatcgt cggtggtatc gcccgtctcg atggtcgtcc
    51001 ggtgatgatc attggtcatc aaaaaggtcg tgaaaccaaa gaaaaaattc gccgtaactt
    51061 tggtatgcca gcgccagaag gttaccgcaa agcactgcgt ctgatgcaaa tggctgaacg
    51121 ctttaagatg cctatcatca cctttatcga caccccgggg gcttatcctg gcgtgggcgc
    51181 agaagagcgt ggtcagtctg aagccattgc acgcaacctg cgtgaaatgt ctcgccttgg
    51241 cgtaccggta gtttgtacgg ttatcggtga aggtggttct ggcggtgcgc tggcgattgg
    51301 cgtgggcgat aaagtgaata tgctgcaata cagcacctat tccgttatct cgccggaagg
    51361 ttgtgcgtcc attctgtgga agagcgccga caaagcgccg ctggcggctg aagcgatggg
    51421 tatcatcgct ccgcgtctga aagagctgaa actgatcgac tccatcatcc cggaaccgct
    51481 gggcggcgct caccgtaacc cggaagcgat ggcggcatcg ttgaaagcgc aactgctggc
    51541 ggatctggcc gatctcgatg tgttaagcac tgaagattta aaaaatcgtc gttatcagcg
    51601 cctgatgagc tacggttacg cgtaattcgc aaaagttctg aaaaagggtc acttcggtgg
    51661 ccctttttta tcgccacggt ttgagcaggc tatgattaag gaaggatttt ccaggaggaa
    51721 cacatgaaca tcattgccat tatgggaccg catggcgtct tttataaaga tgagcccatc
    51781 aaagaactgg agtcggcgct ggtggcgcaa ggctttcaga ttatctggcc acaaaacagc
    51841 gttgatttgc tgaaatttat cgaacataac cctcgaattt gcggcgtgat ttttgactgg
    51901 gatgagtaca gtctcgattt atgcagcgat atcaatcaac ttaatgaata tctcccgctt
    51961 tatgccttca tcaacaccca ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg
    52021 ctctggtttt ttgaatatgc gctggggcag gcggaagata tcgccattcg tatgcgtcag
    52081 tacaccaacg aatatcttga taacattacg ccgccgttca cgaaagcctt gtttacctac
    52141 gtcaaagagc ggaagtacac cttttgtacg ccggggcata tgggcggcac cgcatatcaa
    52201 aaaagcccgg ttggctgtct gttttatgat tttttcggcg ggaataccct taaggctgat
    52261 gtctctattt cggtcaccga gcttggttcg ttgcttgacc acaccgggcc acacctggaa
    52321 gcggaagagt acatcgcgcg gacttttggc gcggaacaga gttatatcgt taccaacggg
    52381 acatcgacgt cgaacaaaat tgtgggtatg tacgccgcgc catccggcag tacgctgttg
    52441 atcgaccgca actgccataa atcactggcg catctgttga tgatgaacga tgtagtgcca
    52501 gtctggctga aaccgacgcg taatgcgttg gggattctgg gggggatccc gcgccgtgaa
    52561 tttactcgcg acagcatcga agagaaagtc gctgccacta cgcaagcaca atggccggtt
    52621 catgcggtga tcaccaactc cacctatgat ggtttgctct acaacaccga ctggatcaaa
    52681 cagacgctgg atgtcccgtc gatccacttc gattccgcct gggtgccgta tactcatttt
    52741 catccaatct accagggtaa aagtggtatg agcggcgagc gtgttgcggg aaaagttttc
    52801 ttcgaaacgc aatcgaccca caaaatgctg gcggcgttat cgcaggcttc actgatccac
    52861 attaaaggcg agtatgacga agaggcgttt aacgaagcct ttatgatgca taccaccacc
    52921 tcgcccagtt atcccattgt tgcttcggtt gagacggcgg cggcgatgct gcgtggtaat
    52981 ccgggcaaac ggctgattaa ccgttcggtg gagcgggcgc tgcattttcg caaagaggtc
    53041 cagcggctgc gggaagagtc tgacggttgg tttttcgata tctggcaacc gccgcaggtg
    53101 gatgaagccg aatgctggcc cgttgcgcct ggcgaacaat ggcacggctt taacgatgcg
    53161 gatgccgatc atatgtttct cgatccggtt aaagtcacta ttttgacacc ggggatggac
    53221 gaacagggca atatgagtga ggaggggatc ccggcggcgc tggtagcaaa attcctcgac
    53281 gaacgtggga tcgtagtaga gaaaaccggc ccttataacc tgctgtttct ctttagtatt
    53341 ggcatcgata aaaccaaagc aatgggatta ttgcgtgggt tgacggaatt taagcgctct
    53401 tacgatctca acctgcggat caaaaatatg ctgcccgatc tctatgctga agatcccgat
    53461 ttctaccgca atatgcgtat tcaggatctg gcgcaaggga tccataagct gattcgtaaa
    53521 cacgatcttt ccggtttgat gttgcgggca ttcgatactt tgccggaaat gatcatgacg
    53581 ccacatcagg catggcagag acagattaaa ggtgaagtag aaaccattgc gctggaacaa
    53641 ctggtcggta gagtatcggc aaatatgatc ctgccttatc caccgggcgt accgctgttg
    53701 atgccaggag aaatgctgac caaagagagc cgcacggtac tcgattttct actgatgctt
    53761 tgttccgtag ggcaacatta ccccggtttt gaaacggata ttcacggcgc gaaacaggac
    53821 gaagacggcg tttaccgcgt acgagtccta aaaatggcgg gataacttgc cagagcggct
    53881 tctgggcgag taacgtgctg ttaacaaata aaggagacgt tatgctgggt ttaaaacagg
    53941 ttcaccatat tgcgattatt gcgacggatt atgcggtgag caaagctttc tactgcgata
    54001 ttcttggttt cacgctgcaa agcgaagtct atcgcgaagc gcgcgactcg tggaaagggg
    54061 atttggcgct taatgggcaa tatgtgattg agcttttctc ttttccgttc ccgccggagc
    54121 gccccagccg accggaagct tgcggcctgc gtcatctggc ttttagcgtt gatgacatcg
    54181 atgcggcagt ggcgcacctt gaaagccata acgtgaagtg tgaagccatc cgtgtcgatc
    54241 catacacgca aaaacgcttc accttcttta acgatccgga cgggctgccg ttggaactgt
    54301 atgagcagta aggcttgtca tcgccgcatt tgcccggtaa cgtgccgggc attgctactg
    54361 taaaatcgca ccatcatgac actcacgctc aatagaccac ttctcacctc acgccagatt
    54421 ctggtggcct ttagcggcgg gcttgactcc accgttctgc tgcatcagtt ggtgcagtgg
    54481 cggacggaaa atccgggcgt cactctgcgc gctattcatg tgcatcacgg tttaagtgcc
    54541 aatgccgatg cctgggttac gcattgcgaa aacgtctgcc aacagtggca ggtgccgctg
    54601 gtggtcgaac gcgtacaact tgcgcaagag gggctgggga ttgaggccca ggcgcggcag
    54661 gcgcgttatc aggcatttgc ccgtacccta ttgcccggcg aagtgctggt caccgcgcaa
    54721 catctcgacg atcaatgtga aacctttctg ctggcgctaa aacgcggtag cggccctgcc
    54781 gggctttcgg ctatggcgga agtttcggag tttgctggaa cacggcttat ccgcccgttg
    54841 cttgcccgca cgcgggggga actggagcag tgggcattgg cgcatggttt acgctggatt
    54901 gaagacgaaa gtaatcagga cgactcatac gatcgtaact ttctgcgcct gcgcgtagtg
    54961 ccgttattgc agcagcgttg gccgcatttt gcggaagcaa cggcccgcag cgccacactt
    55021 tgtgctgaac aagagagtct gttggatgaa ctgctggcgg atgatttagc acactgccaa
    55081 acgtcgcagg ggacgctgca gattgcgcca atgctggcga tgagtgatgc ccgtcgcgcg
    55141 gcgattattc gccgctggct ggcagggcag aatgcaccga tgccttcccg cgacgcgctg
    55201 gtgcgcatct ggcaggaagt ggcgctggcg cgggaagatg cttccccctg tttacgtttg
    55261 ggcgcctttg aaatccgccg ctatcagtcg caactgtggt ggattaaatc cgtcaccggg
    55321 caaagcgaaa ccattgtgct gtggcagacg tggctacaac cgctggaatt accggcgggg
    55381 ctgggaaccg tacagcttac tgcgggaggc gatattcgcc ctccgcgtgc agacgaagcg
    55441 gtcagcgtgc gtttcaaagc gccaggattg ctgcatattg tcgggcgcaa cggcggacgt
    55501 aagctgaaga aaatctggca agagttaggc gtgccgccgt ggctacgtga caccacgcca
    55561 ctgctgtttt atggagaaac gctgattgcg gcggcagggg tatttgtgac gcaagaaggt
    55621 gtggctgaag gtgagaatgg cgtaagtttt gtctggcaga aaacgcttag ttaagtgaaa
    55681 gccggataag acgcatcaaa cgtcgcatcc ggcgaaagtc aatcaggatt cgcttaccac
    55741 caccgtaccg atttcagggt ggctaaagct ggtaatttta tccagacgca gctcgcgggt
    55801 ttcgccagcg gcctcgacga ccagatactc cacatttttg cgggagacta aatcactggc
    55861 ttttgcctgc aatttttcgc catctttcag ctcaagtgtc agcattaaat gatgctggca
    55921 ggcgagctca agattatcgt aatcatcaca attaattggt tgatacgtat cattcattga
    55981 cataatcgct caccagtaag tttgccgcag cgtatgctgc tttttccctg acagcctcag
    56041 aaagggcgtc gtcggcagcc atttcattca gcactttcaa aacgcagccc agcgcgtccg
    56101 gaacgtatcc taagtctccg ctggcgattt ccgcgtaccg cttgcgtatt aactcacaat
    56161 atttttccac atgccctcct gtcagcactc tgacttaacc gtggatgcaa gtctaagcct
    56221 acgaagataa actctgtttc gcaaggtgac tataccacac tcatttctgc aatatcagcg
    56281 ccgcaactgc acgtattccg ttacaatggc ctcctgattc gaaaggagtt ttcttatggc
    56341 gcttaaagcg acaatttata aagcgacggt taatgtggcc gatctcgacc gcaaccagtt
    56401 tctcgatgcc tctctgacgc tggcgcgcca cccttcagaa acccaggagc gtatgatgct
    56461 gcgcttgctg gcgtggctga aatatgctga tgaacgtctg caatttaccc gtggtttgtg
    56521 tgccgatgat gagccggaag cgtggctgcg taacgatcac ctgggcattg atttgtggat
    56581 tgagctgggg ctgccggatg agcgacgaat taagaaagcc tgcacccagg ccgcagaagt
    56641 ggcgctgttt acctataata gtcgggcggc gcaaatctgg tggcagcaaa atcagagcaa
    56701 atgtgtgcag tttgccaatc tttccgtctg gtatctggac gatgaacaac tggcgaaagt
    56761 aagcgccttt gccgatcgta ccatgacgct gcaggcaacg attcaggatg gtgttatctg
    56821 gttatcggat gataagaata atctggaagt taacttaacc gcctggtaac aaccttcatg
    56881 attgtgattt cccgacatgt tgctattccc gatggtgagc ttgagatcac cgccattcgt
    56941 gcgcagggcg ctggcgggca gcatgttaat aagacctcaa cggctattca tctgcgtttt
    57001 gacattcggg cgcccagcct gccagagtat tacaaagagc gtctgctcgc cgccagccat
    57061 catttgatca gcagtgatgg cgtgattgtc attaaggcac aggaataccg cagtcaggaa
    57121 ctgaaccgcg aagccgctct ggcccggttg gtggcagtga ttaaagattt aacaaaagaa
    57181 caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag gctggcatcg
    57241 aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg tcgggaataa
    57301 aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa tcagcctctt
    57361 tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg cgcaggctgc
    57421 cgaactgaaa ccgatgccgc aaagttggcg cggcgtgcta ccgtgtgccg attgcgaagg
    57481 aatcgaaatc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg agcgttatct
    57541 cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc gaaccgctga
    57601 caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga aaggcgatgc
    57661 gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact atacgctgga
    57721 accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt atttttatat
    57781 ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg tagcgaataa
    57841 cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac cggtgttact
    57901 gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc caaccaaagt
    57961 attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta gtttggggca
    58021 gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg cttatcaggc
    58081 ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga taaggcgttt
    58141 acgccgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag cccaacaaag
    58201 cgacccgcct tgatataaat cccaggcttc aggtgcccaa catactgtaa ctgcgtttct
    58261 ggcttaaacg cagagacatc accaatacgc atatgcatca gatcggcagg gctaatccag
    58321 cccgattgcg ccagtgtaag tggatgcccg gctttggcaa atgcatcagt gacaaattcc
    58381 gaacaaaacc acgacttttt gtctccttcg cacacactgc ttaactgcgc tttcgccagg
    58441 tcgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca catctggcga
    58501 gtcaccataa agggaataaa ttcgacaatg ttgcgatagt tataaccgct atctttgatt
    58561 ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg gactcgtaag
    58621 acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacaatctg gacgccagcg
    58681 cctgtcgctt ctgcaacgtt attatcaccc agaaagattg caacgtgact cacagaggaa
    58741 gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg
    58801 ccgggtttga gatcaggtgc cgttatttct tttattgatt gttcggtgaa agagctttga
    58861 tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc tgatgggtct
    58921 ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag aagcaggcgg
    58981 cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc ctgaaattca
    59041 ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga tgtcaccagt
    59101 cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt cgttgtcgag
    59161 gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat cagcaaacat
    59221 cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg cacgcagttc
    59281 gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag
    59341 aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt cgtcgtagtt
    59401 ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac ccatcgtcag
    59461 gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg agtacttggt
    59521 acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg taccctggcc
    59581 atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg gggtagcgac
    59641 atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac cagcagcgaa
    59701 atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt ttaccggacc
    59761 cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg cgaaagtcag
    59821 cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg
    59881 caccagcagc gcaaccagcg ggaagctact gccttcaacc gctttaacca gcagagtctt
    59941 aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt
    60001 cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt tcggcgcgat
    60061 agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc
    60121 ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga tagaaccggt
    60181 gtcggcctgt acggcgcgga aatccagccc catgcggctg aagattttgc tgtaggccgc
    60241 atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag agtaagcatc
    60301 tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa
    60361 cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa gctcgttacg
    60421 aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac gctcgccacg
    60481 gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct cttgccacaa
    60541 atcggctggc tgaaccaccg gcatcgacac ctcgatcgca ccggcgttgt tcatctcttc
    60601 acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc aggtatataa
    60661 cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc tgatcacctc
    60721 ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac gcatgttgtt
    60781 acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa aaagtgattt
    60841 agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg cggttccagc
    60901 gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc atgtagccag
    60961 acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg gtcctgcgcc
    61021 agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt caaaagctgc
    61081 ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact ttgcgcatag
    61141 ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat atccactacc
    61201 ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc tttatggcaa
    61261 acaacctctt tcagctctac cagcgacatg ccaattgggt tagggcggaa agtagagcgt
    61321 gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac agtcggacgc
    61381 cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct gaatgcttcc
    61441 aggccgcgaa cggcgtcggc ctggttgtag gaagcaatga gatgcagttc gccgttggcg
    61501 ctttttacca gacctggctg gcgcggaacg gcgaattttt ctttataggg cgagcgaata
    61561 acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc gcagagccga
    61621 tacatacagc ctgacgatag cagcccggcg taccgctggt gacttcgcag ctatgcagta
    61681 atacagcatt ggctttcatt ttagaggcat tgatttgcat ctgcttacgt gcggttggaa
    61741 tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact tcaccgagat
    61801 cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc ggcgtggcgc
    61861 gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt tgaacgggtt
    61921 cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact aaacagatcg
    61981 gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg aatcaggagc
    62041 ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa ttgtggaaac
    62101 agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt gctgcttcgt
    62161 aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg tctttgttat
    62221 cttcacgcgt tacgatcagg tttacgtatg gggagtcttt atcttcgaca aagataccgt
    62281 ctttcgccgg agtcaggcca atctggctgg cataggtggt attgataact gccagagcga
    62341 tttgcgcgtc gtccagagaa cgcggcagtt gcggtgcttc cagttcaaca attttcagat
    62401 tttttgggtt ctcaacaaca tcaagaactg tcggcagcag gccaacgcca tctttcagtt
    62461 tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt ggatcgtttg
    62521 gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc ttggagtaac
    62581 cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca cgatctttca
    62641 gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg cctttgctca
    62701 atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc aggccatatt
    62761 tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca acaatcacgc
    62821 cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt gccagtgatc
    62881 caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct tattccttct
    62941 tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg atgcggtcgc
    63001 ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta ttcatcaccg
    63061 tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca ccggcaccga
    63121 ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt gccgcattca
    63181 ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt ggcgtggcac
    63241 ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg ttctcgacca
    63301 tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc tgcaaaccaa
    63361 tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag ataatgaacg
    63421 ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc gcgttagcaa
    63481 taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca atcacaaacc
    63541 caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga accagcagcc
    63601 acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc ctgcagccag
    63661 gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat gatgccgaat
    63721 ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac gttgaaacga
    63781 cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc cagacgcagc
    63841 atcggcacgc agtcagtaaa tggctccgtt tgcagacgtt cctggtaatc ttccgggata
    63901 tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg atgcgagaac
    63961 acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc cacgcaatca
    64021 caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa acccagacgg
    64081 cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc cagcgcgctg
    64141 gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc aatcgccaca
    64201 cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc gccaagacca
    64261 accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt gttgtccagc
    64321 tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt aaaatgctgg
    64381 aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag cgtggtcaat
    64441 tcctggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt tacacaacgt
    64501 ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg tccagctggc
    64561 acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg gaacactttg
    64621 gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta gtcgtagcat
    64681 ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca tccagacgtc
    64741 taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg cgatactagc
    64801 gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc aatttttctt
    64861 gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga caactttgaa
    64921 tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt tgcgctggtg
    64981 gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca gtttgaaacg
    65041 ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga tggtatctat
    65101 tattgcccgc atcatccgca ggggagtgtt gaagagtttc gccaggtctg cgattgccgc
    65161 aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga tatggccgct
    65221 tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc gaacgtggga
    65281 acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa cgcggcagat
    65341 tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca aaaaccggcg
    65401 caatgattaa aagatgaacg gttgaaataa aaatgcattt ttccgcttgt cttcctgagc
    65461 cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca cacaaacagc
    65521 cggttcggtt gaagagaaaa aatcctgaaa ttcaggggtg actctgaaag aggaaagtgt
    65581 gatatacgcc acctcgcgac agtgagctga aagc
//
LOCUS       DAJTXY010000009        65034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTXY010000009 DAJTXY010000000
VERSION     DAJTXY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.009"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            132..575
                     /locus_tag="OO566_000670"
     CDS             132..575
                     /locus_tag="OO566_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6604071.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /gene="ybhA"
                     /locus_tag="OO566_000671"
                     /pseudo
     CDS             811..1630
                     /gene="ybhA"
                     /locus_tag="OO566_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OO566_000672"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OO566_000672"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6604072.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTCIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OO566_000673"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OO566_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6604073.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OO566_000674"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OO566_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6604074.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OO566_000675"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OO566_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6604075.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="OO566_000676"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="OO566_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6604076.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="OO566_000677"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="OO566_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6604077.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="OO566_000678"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="OO566_000678"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6604078.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="OO566_000679"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="OO566_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6604079.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="OO566_000680"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="OO566_000680"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6604080.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="OO566_000681"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="OO566_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6604081.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="OO566_000682"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="OO566_000682"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6604082.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OO566_000683"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OO566_000683"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6604083.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13936..14316
                     /locus_tag="OO566_000684"
     CDS             13936..14316
                     /locus_tag="OO566_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6604084.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="OO566_000685"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="OO566_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6604085.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OO566_000686"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OO566_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6604086.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OO566_000687"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OO566_000687"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6604087.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
     gene            complement(17417..17492)
                     /locus_tag="OO566_000688"
     tRNA            complement(17417..17492)
                     /locus_tag="OO566_000688"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="OO566_000689"
     tRNA            complement(17496..17571)
                     /locus_tag="OO566_000689"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="OO566_000690"
     tRNA            complement(17623..17698)
                     /locus_tag="OO566_000690"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="OO566_000691"
     tRNA            complement(17701..17776)
                     /locus_tag="OO566_000691"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17913..17988)
                     /locus_tag="OO566_000692"
     tRNA            complement(17913..17988)
                     /locus_tag="OO566_000692"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17953..17955),aa:Lys,seq:ttt)
     gene            complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="OO566_000693"
     CDS             complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="OO566_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6604088.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18954..19475)
                     /gene="pal"
                     /locus_tag="OO566_000694"
     CDS             complement(18954..19475)
                     /gene="pal"
                     /locus_tag="OO566_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6604089.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="OO566_000695"
     CDS             complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="OO566_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6604090.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20935..22191)
                     /gene="tolA"
                     /locus_tag="OO566_000696"
     CDS             complement(20935..22191)
                     /gene="tolA"
                     /locus_tag="OO566_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6604091.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAK
                     AAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADI
                     NNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAA
                     KLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22256..22684)
                     /gene="tolR"
                     /locus_tag="OO566_000697"
     CDS             complement(22256..22684)
                     /gene="tolR"
                     /locus_tag="OO566_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6604092.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22688..23380)
                     /gene="tolQ"
                     /locus_tag="OO566_000698"
     CDS             complement(22688..23380)
                     /gene="tolQ"
                     /locus_tag="OO566_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6604093.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23377..23781)
                     /gene="ybgC"
                     /locus_tag="OO566_000699"
     CDS             complement(23377..23781)
                     /gene="ybgC"
                     /locus_tag="OO566_000699"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6604094.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23931..24224)
                     /gene="ybgE"
                     /locus_tag="OO566_000700"
     CDS             complement(23931..24224)
                     /gene="ybgE"
                     /locus_tag="OO566_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6604095.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24224..24337)
                     /gene="cydX"
                     /locus_tag="OO566_000701"
     CDS             complement(24224..24337)
                     /gene="cydX"
                     /locus_tag="OO566_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6604096.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24352..25491)
                     /gene="cydB"
                     /locus_tag="OO566_000702"
     CDS             complement(24352..25491)
                     /gene="cydB"
                     /locus_tag="OO566_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6604097.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25507..27075)
                     /gene="cydA"
                     /locus_tag="OO566_000703"
     CDS             complement(25507..27075)
                     /gene="cydA"
                     /locus_tag="OO566_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6604098.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27924..30557)
                     /gene="mngB"
                     /locus_tag="OO566_000704"
     CDS             complement(27924..30557)
                     /gene="mngB"
                     /locus_tag="OO566_000704"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR6604099.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKIWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30575..32491)
                     /gene="mngA"
                     /locus_tag="OO566_000705"
     CDS             complement(30575..32491)
                     /gene="mngA"
                     /locus_tag="OO566_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6604100.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWESVDGPEAIDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTVQQDT
                     QPVKSVKTELKQALLSGISFSVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMQ"
     gene            32661..33383
                     /gene="mngR"
                     /locus_tag="OO566_000706"
     CDS             32661..33383
                     /gene="mngR"
                     /locus_tag="OO566_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6604101.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33487..34356)
                     /gene="sucD"
                     /locus_tag="OO566_000707"
     CDS             complement(33487..34356)
                     /gene="sucD"
                     /locus_tag="OO566_000707"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6604102.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34356..35522)
                     /gene="sucC"
                     /locus_tag="OO566_000708"
     CDS             complement(34356..35522)
                     /gene="sucC"
                     /locus_tag="OO566_000708"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6604103.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35616..36833)
                     /gene="odhB"
                     /locus_tag="OO566_000709"
     CDS             complement(35616..36833)
                     /gene="odhB"
                     /locus_tag="OO566_000709"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6604104.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36848..39649)
                     /gene="sucA"
                     /locus_tag="OO566_000710"
     CDS             complement(36848..39649)
                     /gene="sucA"
                     /locus_tag="OO566_000710"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6604105.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39950..40666)
                     /gene="sdhB"
                     /locus_tag="OO566_000711"
     CDS             complement(39950..40666)
                     /gene="sdhB"
                     /locus_tag="OO566_000711"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6604106.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40682..42448)
                     /gene="sdhA"
                     /locus_tag="OO566_000712"
     CDS             complement(40682..42448)
                     /gene="sdhA"
                     /locus_tag="OO566_000712"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6604107.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42448..42795)
                     /gene="sdhD"
                     /locus_tag="OO566_000713"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42448..42795)
                     /gene="sdhD"
                     /locus_tag="OO566_000713"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6604108.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42789..43193)
                     /gene="sdhC"
                     /locus_tag="OO566_000714"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42789..43193)
                     /gene="sdhC"
                     /locus_tag="OO566_000714"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6604109.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43574..43681
                     /locus_tag="OO566_000715"
     CDS             43574..43681
                     /locus_tag="OO566_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604110.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43887..45170
                     /gene="gltA"
                     /locus_tag="OO566_000716"
     CDS             43887..45170
                     /gene="gltA"
                     /locus_tag="OO566_000716"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6604111.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45561..46127
                     /locus_tag="OO566_000717"
     CDS             45561..46127
                     /locus_tag="OO566_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6604112.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATCVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46178..48637
                     /locus_tag="OO566_000718"
     CDS             46178..48637
                     /locus_tag="OO566_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6604113.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALALEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGQVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48652..49383
                     /locus_tag="OO566_000719"
     CDS             48652..49383
                     /locus_tag="OO566_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6604114.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49380..50442
                     /locus_tag="OO566_000720"
                     /pseudo
     CDS             49380..50442
                     /locus_tag="OO566_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50594..51640
                     /locus_tag="OO566_000721"
     CDS             50594..51640
                     /locus_tag="OO566_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6604115.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51637..52428)
                     /gene="nei"
                     /locus_tag="OO566_000722"
     CDS             complement(51637..52428)
                     /gene="nei"
                     /locus_tag="OO566_000722"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6604116.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52464..53198)
                     /gene="pxpA"
                     /locus_tag="OO566_000723"
     CDS             complement(52464..53198)
                     /gene="pxpA"
                     /locus_tag="OO566_000723"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6604117.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53188..54120)
                     /gene="pxpC"
                     /locus_tag="OO566_000724"
     CDS             complement(53188..54120)
                     /gene="pxpC"
                     /locus_tag="OO566_000724"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR6604118.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(54114..54770)
                     /gene="pxpB"
                     /locus_tag="OO566_000725"
     CDS             complement(54114..54770)
                     /gene="pxpB"
                     /locus_tag="OO566_000725"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6604119.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQQHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54793..55536)
                     /gene="ybgI"
                     /locus_tag="OO566_000726"
     CDS             complement(54793..55536)
                     /gene="ybgI"
                     /locus_tag="OO566_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6604120.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55807..57288
                     /gene="dtpD"
                     /locus_tag="OO566_000727"
     CDS             55807..57288
                     /gene="dtpD"
                     /locus_tag="OO566_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6604121.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57438..58856)
                     /gene="phrB"
                     /locus_tag="OO566_000728"
     CDS             complement(57438..58856)
                     /gene="phrB"
                     /locus_tag="OO566_000728"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6604122.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYETARKGK"
     gene            complement(58853..59362)
                     /locus_tag="OO566_000729"
     CDS             complement(58853..59362)
                     /locus_tag="OO566_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6604123.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59491..59808
                     /locus_tag="OO566_000730"
     CDS             59491..59808
                     /locus_tag="OO566_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6604124.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(59863..60243)
                     /locus_tag="OO566_000731"
     CDS             complement(59863..60243)
                     /locus_tag="OO566_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604125.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     EEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYTL"
     gene            complement(60246..64910)
                     /locus_tag="OO566_000732"
     CDS             complement(60246..64910)
                     /locus_tag="OO566_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6604126.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGGTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYWFRHDVLDRLAEQINPDGCCQSYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLLEIRRQRHETDAPEVIRFSYD
                     RLGRMLSEETAQGILTHQYDELGNRTATTFPDGRTQRHLYYGSGHLQQINLDREVISE
                     FTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGMRGVVPETFTDRQYSYSGEDE
                     LLKTRHSRRGETDYFYDPTGSITACRSEDEGYLASWQYDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TGNKTKAGSYHWDDIKDPSVTKPPYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPR
                     ET"
BASE COUNT        15708 a        17640 c        15931 g        15755 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 accacttgta atcaattgat tataaaggat taattcataa agagcattta cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgcag gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gatgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag atgacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgccctct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggcgga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tataccaacc gcatcgccaa tagtcgttat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattacagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagtatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcgccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatga gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgtcgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctaga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctttcctaca atttctaact gtaattcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca cgccaccatt cagatggaat
    15301 atcaaccttg tcatgggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 accgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggcca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgtttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgcaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcgc attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttacattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtggtgggtc
    17821 gtgcaggatg actcgacttc gtctcgccct tcggcgccgt tgctgatgca acgttatcct
    17881 tcacatttta ctaccgcttc caccttagcg attggtgggt cgtgcaggat tcgaacctgc
    17941 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccac tttttcgttg
    18001 ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt tcagacttga
    18061 gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg acacgaccag
    18121 aaataatgcg acttctggtc gtgttttatg cattacatcg cgttcagacg tttttgtgcc
    18181 tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac ggctttcgct
    18241 tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc atcagcagcc
    18301 tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc atcatctttt
    18361 ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag gtaagttgaa
    18421 tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg gcgggattta
    18481 tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcgccgct tttcaccggc
    18541 gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc cgcaccgctt
    18601 tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac
    18661 aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc ctgaatctga
    18721 ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag ttgggttaaa
    18781 agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc gaccgagcct
    18841 gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc tataccaacc
    18901 agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa
    18961 accagtaccg cacgacggtt tttggagtat gctgcttcgt catgacccag tactgcaggt
    19021 ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt accctgcagg
    19081 tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc cggagtacca
    19141 cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg caggaagttt
    19201 gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc cagatcgaag
    19261 taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc ttcggaagac
    19321 atgttgccgt tgccgccgtt cgcatccata ccagtgccag cacccagcat gccttcgctg
    19381 ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac aggcagagca
    19441 atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt actattcaat
    19501 taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat cagttgccgg
    19561 aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc ccatcccctg
    19621 agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt catccaggaa
    19681 cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg
    19741 cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg
    19801 gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat tcactttata
    19861 aacctgcgga cgaccggcct ggtcagaagt aaatgccagg ttctggctat ccgggaacca
    19921 ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac cagaagccaa
    19981 atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca gtttgctgcc
    20041 gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct gacgtacagc
    20101 gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg tcacataagc
    20161 cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg aacggtgaac
    20221 gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga actgaccgcc
    20281 gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc cggtcagttt
    20341 ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct tgttcacttt
    20401 gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc
    20461 aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag cgtcaatgcc
    20521 cagtgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct gtggcagacg
    20581 agcgcgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa cgatgccgcc
    20641 aatatcttca ggtgccgcac caggccccgc ccactggaaa ggaacaacac caataggacg
    20701 accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa ctgatgccca
    20761 cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc ccttatctgg
    20821 accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca aaactaccag
    20881 aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac agtcaacatc gcgattacgg
    20941 tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg gtggtttcgg
    21001 gatcttcgca agtttagctg ctgccaacgc agcctgacaa agtgcgggat cgccaccttc
    21061 aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg tacaggtttt
    21121 gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa tctgcccggc
    21181 atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac tcccggcagg
    21241 cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac cagagcttag
    21301 ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt tttcagctgc
    21361 tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg ctttcttatc
    21421 agctgctgcc ttatcagcag ccgctttttc tgctgctttt ttgtcggctg cagctttctc
    21481 tgctgccgct ttcttatcag ctgcagcctt atcagctgca gccttttcag cagccgcttt
    21541 cttctcagct tctgccttgg ctttttcagc agcttcagtt gccgctttct ttcttgcttc
    21601 agctgcagct gcttctgccg cttccgcttt cttcttcagt gccgcagcgg ctacctcggc
    21661 ttttttctgc gcttcggctg cggctttggc ggcttctgct tctgcttttt tcttcgcgtc
    21721 tgcagccgct ttcttcgcag cttcttccgc agctttagca tctgccgccg ctttcgctgc
    21781 cgccacttca gcttgcttct gctttaactc ggcctgtttt gcggcttctt cagcctgctt
    21841 tttctgctcc tgagccgcta accgctcttt ctcaagttgc ttcaggcgtt cctgttcagc
    21901 cgcttgtttc tcacgcagtt cttcagcagc ctgctgttcc ttcatcttgc gctgttcatc
    21961 agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa ctaccgcacc
    22021 tgaatcaacc atgacagcgt cgatggacga accaccgccg cctccggctg aagcttctat
    22081 attctcatcg aacgaactcc agatcagcgc cgcaaataag atgacatgca gcactgctga
    22141 aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca ctctcggttt
    22201 ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca gatgtttaga
    22261 taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag ttcagtgctt
    22321 taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc gttttcgggt
    22381 tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa cgctccaggc
    22441 gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca atcactggcg
    22501 gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg acctccacgc
    22561 tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc agtacgtcca
    22621 gcaacggtac aatgttgatt tcggacttga gatcgcgacg acctcgtcca cgcgctctgg
    22681 ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc agaatcgcgg
    22741 taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc tggttgagac
    22801 ggttataagc cattaccgcc ggaattgcgg cgaacagacc aattgccgtc gcaatcaacg
    22861 cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc ccgagggcga
    22921 taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg ctgatggagc
    22981 caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacgg ttcatggaga
    23041 tacgcatagc acgcgacgcc ccttccacta cggcttccgg cgcatggcta ttggcacgat
    23101 gcaggcgcac aaactctttg aacccgctgt aaaagatttg ttccgaacca gtcagattat
    23161 cccgtttccc ctgactctct tgatagaggc gagagagttc gattccagac cagaatttat
    23221 cttcaaacgc ttcggcttca cgcgccgctg cgttaagaat acgggttcgc tggataataa
    23281 tggcccagga tgcgattgaa aaaccaatca aaatcaacat gataagttta accagaaggc
    23341 tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact ccgcgacaat
    23401 agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa ccagaacctc
    23461 tgcttcattc agcaaagtat tctcggcgtt gacaatatgt tgcgtgaaaa ccaaagaggt
    23521 gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc gcgcaggtgc
    23581 ataatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca tcagcgcctg
    23641 ctgactgaag tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa aagcgacgta
    23701 actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc gaaccggcca
    23761 tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta gagcttttaa
    23821 acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaagtta agggagcgta
    23881 aaaatttatg ggcctagagc ggcccataat tttttaaaga gaattctgat ttaaaagaag
    23941 aagaaaatca gcccgacaat gagaacaata tcggcaagca atgggcaaaa aatcccttgc
    24001 caaagaacct tctgcggacg aaagcccacg ccgtgaatca caccagcaca gacggcccac
    24061 atcagcaata aaccatgcca gatttccagt tcactggtct tcgcggcaaa acgagatggg
    24121 tcccaaaaca tacatcctgc taacagaagc gccatcacga aggaaagcgc ccgcaggggg
    24181 cgcttgtcca ttaccgcata caaggtcgcg ataatcttac tcatcagatg tcttcttgac
    24241 cggctttgcc tgattcgacg tgttcaagcg ccagcgcggt gattacccca aacgaacagg
    24301 caagaagcgt tcccagaatc catgcgaaat accacatttt tagctcctta cttagtacag
    24361 agagtgggtg ttacgttcaa tatcttcttt ggtgatacga ccgaacattt tccagtaaca
    24421 ccaggcggtg tagagcagaa tgatcggtac cagaaccacc gcaacccagg tcatgacgtt
    24481 aagcgtcagc tggctggaag ttgcatccca cattgtcaga cttgcgttca tcatggtgct
    24541 ggacggcatc acaaacggga acattgcgat accggctgtc aggatgatgc aggccagcgt
    24601 cagggaggag aacacaaacg cccacgcggc tttatccata cgtgcagtca ggatggtcag
    24661 cagcggcaga accacaccca gtgccggaat agcccacaga attggcgtgt tgttgaagtt
    24721 aaccagccat gcgccagctt cacgaaccac ttctttattc agtgggttag aggctgcgta
    24781 atggtccatt gtcgatttca cgacataacc atcgataccg tacatcaccc atacgccagc
    24841 cagtgcgaaa cagaccagtg tcaccagcgc agccacctga gccgttgcac gggtacgcag
    24901 gtgcagttcg cccccggtac gcatttgcag ataggttgcg ccctgagtaa tgatcatccc
    24961 tacgctcacc acgcctgcca gcaggccgaa cgggttaagc aactggaaga agttaccggt
    25021 gtagtacaga cgcagatatt catcaacgtt gaacggtacg ccctgcaaca ggttaccgaa
    25081 cgctacacca attaccagcg gcggaacgaa gctaccaatg aagatgcccc agtcccacat
    25141 gttacgccag cgggtttctt caatcttgga gcggtagtca aaaccgaccg gacggaagaa
    25201 caaagacgcc agcacgagga tcatcgccac atagaagccg gagaacgcag cggcatagac
    25261 catcggccag gcagcaaaga gtgcgccgcc cgcggtgatc agccaaacct ggttaccgtc
    25321 ccagtgtggt gcaatggagt taatcataat tcgacgctcg gtgtcgttac gaccgaggaa
    25381 acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg actgcaaaac caatcagcag
    25441 aacgccaacc agcagccacc agataaaacg caatacttca taatcgatca tttgacgact
    25501 cctgtcttag cgtgccggct gagtagtcgt ggaagactgc tcaaagtgat agcgaccggt
    25561 tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt aagaacaatt ctgccaccag
    25621 gaacagggta tacaggccgc aaatcagcac cattgagaag atgagatcgc ctgcggtcag
    25681 tgacgaattc gccacagctg tcggcagcac ttcaccgata gcccacggtt ggcggccata
    25741 ttcagccacg aaccagcccg cttctacagc aatccacggc agcggaatac cgtacagcgc
    25801 ggcgcgcaga agccattttt tctcgccaat gcggttgcgg atgacactcc agaaagagag
    25861 cgcgatgatt gccagcagca ggaagccaca cgccaccatg atacggaacg caaagtacag
    25921 cggcgctaca cgcgggatgg agtctttggt tgcctgttga atctgcgctt cagtcgcatc
    25981 agccacgttt ggcgtatagc gtttcagcag cagaccgtaa ccgaggtctt tcttcatgct
    26041 attgaactgg tcacgaaccg cctggtcggt agaaccagaa cgcagttgtt cgagcagaga
    26101 gtacgccttc atcccgttac gaatgcgttc ttcatgctgc accatcagct ctttcaggcc
    26161 gataaccggg gtatccacgg aacgcgttgc aatgatgccc agtgcgtaag ggatctgaat
    26221 cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg aacaaagtaa aggcagcagg
    26281 cgcaggttgc gtttcccact cggcttcaat agcagccagt ttggttttct gcacgtcgcc
    26341 catttcgtag ccggattcat cacccagaac aataacagac agaacagcag ccataccgaa
    26401 gctggcagcg atagcaaagg agcgtttagc gaaggcgaag tcacgacctt tcagcatata
    26461 ccatgcgctg ataccgagga tgaacatcgc gccagtcaca taaccagacg ctacagtgtg
    26521 aacgaatttc acctgagcaa ccgggttaag caccagctcg gagaagctca ccatctccat
    26581 acgcatagtt tcaaagttga aatcggacgc gattgggttt tgcatccagc cgttcgcaat
    26641 cagaatccac agtgcggaca ggtttgaacc gagcgccacc agccaggtga cacacatatg
    26701 ctgaacttta cccagacgat cccaaccgaa gaagaacaga cctacaaagg tggattcgag
    26761 gaagaaggcc atcagacctt cgattgccag cggcgcaccg aagatatccc ctacatagtg
    26821 ggaatagtaa gaccagttag tcccgaactg gaactccatg gtcagaccgg tagccacacc
    26881 cagagcgaag ttgataccaa acaacttgcc ccagaacttg gtcatatctt tataaatctg
    26941 tttgccggag aggacgtaga ccgtttccat aatggccagg aggaacgcca taccgagcgt
    27001 cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca aactgtaagc gcgacagttc
    27061 gactatatct aacatcatga ctccttgctc atcgcatgaa gactccgaga gtgaaccccg
    27121 ttagaaaggg tcacacgcat gccccaatag aaatttgttt gaacactttc atcataccat
    27181 gtgaatgcac acaaaaacgg cgtaaagaaa ggttgcaaac atgttaataa aaactcaaat
    27241 tgatcccacg tatatattac gccgcaaaat ccttacaata aacaggtttt tgttgaagca
    27301 aatttgcatt tttcgacggt gatcaattta tagctaaatt gccgcctttc agccaatttg
    27361 atcgagaaca atttatctct ttttgatgcc catttccaag acttatacat tgataaatat
    27421 caatttctac aaattatgtt aatcatgttt caacaaggta actttagtga catttatgtt
    27481 taaaatgtgt gaattataag actgaaatta gtttcggttg gcaattaaag ataaaaagag
    27541 acgaagcaaa aacagcacgg ttgaccttaa ttcttttttt ggtcactggc taatacgacg
    27601 cagagtttca ggattattaa taaaggcata aatctcctcc ccgccgatat taacatttaa
    27661 ttaacttata attatattat aattaactca cccggggtta ttattttgtt aaaaataaag
    27721 ccacatatac gtagcttgag agtgaagata aacgcaaaat tacctgatgc gctacgcttg
    27781 ccagtgtcta catcatctct gcaatatatt gaatttgcct gcttttgtag accgaataaa
    27841 gcgttcatgc cgtatccggc atgaacaaag cgccctttgt caacaatctg gccgcgcata
    27901 tgtggcttcg gtttgattgc tattcaggca agccggtaac tgaacgtccg tgactggccg
    27961 ggtaaaaaag gccccgatag atttgaacct tgattttcct cggtggtaat gtgttcatcc
    28021 atcatcgttt ctgagcaaga aatcacctcg cgactgaaag caacagtcgc atcacaggtt
    28081 gctgattcag ccggattaaa cagccgtaaa atcacttctt gtcggtcttc agctttctta
    28141 agtgcgctta tcaggcatcc cactgggggc attttcaaca aactataact ttccggcacg
    28201 ttgaatccgg ctttgttgag cttcatcgca tcccatggga ttttgttgta acactgtact
    28261 ggagtcagcc atgctcgcgc ctgctgagct acaccagcgg cggttggcgt accggtataa
    28321 ctcaataaac ttaggcgaca agaaagcaga ccacgtagtt gtgagtccgg gactggcatt
    28381 ttaattcccg aaggccgcct aggccttaaa agcagatctt ctttccccag taagcccacg
    28441 ccgcgcagca acgtaatggc aaaggttttc ttctcttcac cgatgacttc aaattcacgt
    28501 agcccttcgc taaagacagc catgccgtta cgcccttcct gtaaggctgc atagttgagc
    28561 atattccata ccggaaccgg cgcttctttc cagccttctt gctgccagtt gttcattgca
    28621 ctgtcgttca cggggcgcgt tagcgaacca aactgggtat ctgccagaac actatcggtg
    28681 ttaaaagatg tagggatcag gacgcgaagg cgatgatcgt cagcctggtt atcaaggttg
    28741 atatccacat caatgcgcct gttgttatga ctaagagtga caaccaactc gacacctact
    28801 ctgccagtgg attgtcttgc gctgcgttgt tgcagattac gcggcaccgc catttcatag
    28861 cggataacag ccctgctctg ccaggcctca tgaataatct cgcattgcgg tttcgcattc
    28921 gccgaagtaa ttacccactc ttcttttgcg ggtgaatagt catattcatc accatcatca
    28981 gagctttctt caatttgcaa cacccggtca tagcgcacac cgctatcttt atctaccagt
    29041 tgtagcgttc catcctcatt gagcgcaatt tgccagaaag cattttccag tatcccttca
    29101 gcgtcacttt ttgccgcaat cacgttgcca ggctgattcg cttcgatata aagcgtgcga
    29161 tagcccatag aagggacaat ctggttgatc tgtatatcaa actccataaa gggatcgtaa
    29221 ttaccgtaat gaactatttg ccgatcgatt aggcctggat cgatctcacg ggcatggcga
    29281 ataaaatacg gtacaggctg accgcgatcg tctcgtagat taaactggct ggcgcgcagc
    29341 cgcacagtgg tgttgatcac ttcttcccgc ggccagggca tcaggttaaa caggacgagt
    29401 ttgtcggcgt cgctctgcgg catgttgtcg gcgattttac gcatatagaa acgcagcaga
    29461 ttatccgcca tgtcttcagc cagttcgaag cgggcgacga tttcgcgatg aactttgtca
    29521 ctacagcagc agccgatgct gtcgtgggca tgatttttta agatctcttt ccatattttc
    29581 tccagcaagc cgtggtggta ttcaaaaccc aacgtccagg ccagcgttgc cagcggttcc
    29641 agcagattaa caatcttatt ttcaatacgt gcgtgggcaa ctttgatatc catgcgcgtg
    29701 gaaccgatgg tgcgatgcac gcgcatatat ttgccatcaa taaattcccc tttcagggtt
    29761 gccagattat ctcgctgcgc ttcgatcttc tcaaatacct cttcaaagcg gctcatcaca
    29821 aactttcgtt gaggataaat ttcgcgcagt ttctccatca cttcgaagat attttgctgc
    29881 aatggcatct gatcatgccc attcggcagc aaaatctctt tggttacaga cgctttttcc
    29941 agcacgtcaa aataactgtc gaggcgtttg cggaatccgt tttcgtccgc aggtaagtac
    30001 ttaccgatgg cataacccaa cggcatcacc tgcgccgtca cttcgctacc gtcactgctt
    30061 tgccacaaaa actcggtttt atccgtaccg tggcgctcag aacatccacg ccagaacatg
    30121 gtgcaggtaa tgccaaatcc attgtagata tgcggcagtt gcccggacat gccaaaggaa
    30181 tcgggtaaat aacctatttt catcggctcg ccaaacgcga ggcagtcacg cattccgtac
    30241 atcagattac ggacgatgga ttccgcagaa acaatcgtgg tatcggtctg ggtataccag
    30301 gggccgataa tcaacttgcc ggcttctacc agtttcttca cgcggtcttt gttttccggt
    30361 ttcaccgcga aataatcttc gaggatcgcc gtttgcccgt cgagtacgta atatttgtat
    30421 tcgttgtcct gttccagtcg gcacaggatc tcttccatat tattgaccag cagaatgcgt
    30481 gactcttcgg tggtgaaata ccactcccga tcccagtgca tatgcggggt gatgtgaacg
    30541 cgagataccg ctttcatcgt cgtttccttt ttgtttattg cattacgcca tcagtcagat
    30601 agttgccatg cttaaccgcg tgacgccgcc agatcaggag aattgcagtc gagattgcag
    30661 cccccaccaa tgccgcacca aaccagccaa ttgccgccat aacaccgccc gcgccattat
    30721 catgaagtaa aaagagcgag aaaatgccag caccgggtgt cgaaaggccg atattcatcg
    30781 caccgacaat agcgcccgtt accatagagc ccagcacaaa cgaaccaata acccgcagcg
    30841 gatcttcaat cgccatcggg atcgcccctt cggtaatacc tgccagccct aacagccagg
    30901 tggatttccc ggtctcaatt tcaaactctt taaacaggcg cggtgcgagc atcgtggaag
    30961 cggttacggt aaatgccgaa accattttga cggaggcgaa aatggcatac gggccgtaaa
    31021 cgccgttcgc cattgcgccc aggcagaatg cataagcggc tttattcact ggccctccaa
    31081 ggtcaaagga acacataaaa ccgagaatgg cacccagcaa cagcgcgtta cttcctgaca
    31141 gaccgttcag ccaggcggta agcgagttat tgatccaggc gacaggttcc cccaccacaa
    31201 acagcatcag actgcccgct cccaatgtac cgagcaccag gtagagataa aaagtcagga
    31261 agccattgaa tttactgcta agacgcaagt gatttttcat ccagcgtatc aagtaaccgg
    31321 ctatcaatcc gccaacgacc gcgccgagaa acccggagcc gatcatgttg gcggcaagtc
    31381 cagccgcaaa gcctggcgct aacgccggtt tatctgccag cgaataggcg gtataggctg
    31441 ccagcaccgg taccatcaaa attccgagca gcccgccgcc cagtttgcgg tacatccaca
    31501 gccaggagtt ttcttcatta aacagatctt gtagcccgaa gatttgcgac agtaataccg
    31561 cgaccgccag caccgtcccc cccgcgacaa tcaacggtac ggaaaaagag atcccgctca
    31621 acagtgcctg tttcagctcc gttttgacgc ttttcaccgg ttgcgtatct tgctgtacgg
    31681 tacgcgtctc atcgccacgc tcgagggtaa gcgattgttg gatcaacgct tctgcatggc
    31741 gaatcggctc ggcaacaggc acagaaagcg caggaatgcc gttaaaacgc tcactctcct
    31801 tgatggcgac ttcagccgca aaaatacagg cagttgcact attgagttga tccgccgtta
    31861 aacgcccttc aataccgtta gcaccttgct tttcaacgta gacattcacg ccgagtttgc
    31921 gtccggcttt ttccagatac tccgcagcca tgtaggtgtg agcaataccc gccgggcagg
    31981 ccgttacgca aacgatagtt ggcgcgttgg aaaaagaggc agaaggttgc gtgcctccct
    32041 tgtcatccag cgccgagagc aattcatcag gcgtcgtcgc cgactgtata cgcgcccgaa
    32101 tctcatcatc cgccaggcgc gtggtcagcg ctgtcagcag ttgcatgtgc gtagtacccg
    32161 cttcattggg gggaatcgcc agcaacacca ctaaatcaat cgcttccggg ccatccacgc
    32221 tttcccactg aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct tttaccgcag
    32281 cagttttgcc atgcggcaca gccaaccctt cacctaaggc ggtcgggcca aggctttcac
    32341 ggcgatacac ttctttcaga aattgctcag tactggaaat tttccccaga gcagcaagac
    32401 gttgagtcaa cgcgtgaatg gcctcttcac ggctggtaaa gcgcgcattc agacacaacg
    32461 catcgcggtg ggtcagagtc gtcaggttca tcatgatcct cacttgatcg tttttatagt
    32521 cgcgccagtg tgcccgataa aacaatacca ttgcgtaatc aatttcactt gattgaaatt
    32581 atttgcattg tatttgtatt atttacagga tgaatttatc gggcataatg gctaaattcg
    32641 tattaatgag atgctaaata atgggacaca aacccttata ccgacagatt gctgatcgca
    32701 ttcgtgagca aattgcgcgt ggcgagctta aacctggcga tgcgttacct accgaatcgg
    32761 cattacaaac agagtttggc gtcagccggg ttacggtgcg tcaggctttg cgccagttag
    32821 tcgagcaaca gatcctcgaa agcattcagg gcagcgggac ttacgtcaaa gaagagcggg
    32881 tcaattacga tatttttcag ttaaccagtt ttgatgaaaa actgtcggac cgtcacgtcg
    32941 atacacacag tgaagttctg atatttgaag tgattccggc tgacgatttt cttcagcaac
    33001 agctacaaat caccgtgcag gatcgtgtgt ggcatgtgaa gcgcgtgcgc tatcgcaagc
    33061 aaaagccaat ggcgctggaa gaaacctgga tgccgcttgc gttgttcccg gatctcacct
    33121 ggcaggtcat ggaaaattcg aaatatcact ttattgaaga agtgaagaag atggttatcg
    33181 atcgtagcga acaggaaatc attcctctga tgccaaccga agagatgagc cgtctgctta
    33241 atatcagcca gacaaagccg attcttgaaa aagtatcgcg tggatattta gttgatggtc
    33301 gggtgtttga atatagccgc aacgctttta ataccgatga ctataaattt accctgatag
    33361 cgcagcgaaa atcatcgcga taagcacaaa aaaggccatc atacgatggc caaccatgtc
    33421 gaaaccgaca gcccttcgcc attcctgtag agggaagcgg cgagggctat ttcttattac
    33481 agatatttat ttcagaacag ttttcagtgc ttcaccgata tccgccaggc tgcgaacggt
    33541 tttcacgcct gcggcttcca gagcagcgaa tttctcatcc gcagtccctt tcccaccggc
    33601 aatgatcgca cccgcgtggc ccatacgttt gcctttcggc gcagtcacac cagcgatgta
    33661 acccacaact ggcttggtaa catgctcttt gatgtacgct gccgcttctt cttcagcgct
    33721 accgccgatc tcaccgatca tcacgatcgc ttcggtctgc ggatcttttt cgaacatttc
    33781 gagaatgtcg ataaagttag agccagggat cgggtcaccg ccgataccga cacaggtcga
    33841 ctgaccgaaa ccgtaatccg tggtctgttt aaccgcttca taggtcagtg taccggaacg
    33901 ggaaacgata cccactttac ccggtttgtg aatgtgaccc ggctggatac cgattttgca
    33961 ttcacccggg gtgataacgc ctgggcagtt cgggccgatc atacgaacgc ctgcttcgtc
    34021 cagtttcact ttcacggtca gcatatccag cgtcgggatg ccttcagtga tggtgataat
    34081 cagtttgatg cctgcatcga tagcttccag aatggagtct ttgcagaacg gtgctggtac
    34141 gtaaataaca gacgcggaag cgccagtagc agcaacagct tcacgcacgg tgttgaacac
    34201 cggcaggccg aggtgggtag tgccgccttt acctggagtt acgccaccaa ccattttagt
    34261 gccgtatgca atggcctgtt ctgagtggaa agtcccctgg ctaccggtaa agccctggca
    34321 gataaccttg gtgtttttat caattaaaat ggacattatt tcccctccac tgcggcaaca
    34381 acctgctgag ctgcatccgt cagacctttt gctgcaataa tattcaggcc gctgtcagcc
    34441 agtttcttcg cgccgagttc ggcgttgtta ccttccagac gtaccacgac cggtacgtta
    34501 acacccactt ccgctaccgc gccgatgata ccgtcagcga tcaggtcgca acgaacgata
    34561 ccgccgaaga tgttaaccag aacggctttc actttgtcgt cagagaggat gattttgaac
    34621 gcttcggtta cacgttcttt ggttgcgccg ccgccaacgt ccaggaagtt agccggttcg
    34681 ccgccgtgca gtttaactat gtccatcgta cccatcgcca gacctgcgcc gttaaccata
    34741 caaccgatgt taccgtccag cgcaacgtag ttcagttccc actgtgcagc ctgtgcttca
    34801 cgcggatctt cctgcgactg gtcacgcatt tcgcgcagat caggctggcg gaacagggcg
    34861 ttgccatcag cgcccagttt gccgtcgagg caaatcagat cgccctgttt ggtgatgacc
    34921 agcgggttga tttcgatcag cgccaggtcg cgctccagga aaatggtcgc caggcccatg
    34981 aagattttgg tgaactgctg aaccagttta ccttccagac ccagtttgaa cgccagctcg
    35041 cgtccctgat acggcatcgg gccagtcagc ggatcaagcg caactttatg gatcaggtgc
    35101 ggagtttctt ccgccacttt ttcgatttcc acgccgcctt cggtggaggc cataaagacc
    35161 acgcgacggg aactacggtc tacaacggca ccgagataca gctctttagc gatatcggtc
    35221 gccgcttcaa ccagaatctg gttaaccggt tggccattgg catctgtttg atacgttacc
    35281 agacgcttgc ccagccagtt ttctgcaaaa gcacggatgt cttctttgct gtttacaact
    35341 ttcacaccgc ccgctttacc gcggccacca gcgtgaacct gacatttcac tacccacgga
    35401 ccggcaccga tttttgaagc ggcttcttct gcttcgcgcg gagtagtaca ggcataaccc
    35461 accggtgctg gtaagccata gcgggcaaac agttgttttg cctgatattc atgtaagttc
    35521 atgtgttctg tccatccttc agtaatcgtt atcttttaaa ccgtagaccg acaccctatc
    35581 cacgtcggtc tacagtgcag gtgaagctta aactactaca cgtccagcag cagacgcgtc
    35641 ggctcttcca gcaactcttt gatcgttacc aggaagccca cggattcgcg accatcgatc
    35701 agacggtgat cgtaggacag cgccaggtac atcatcggca ggatctcaac ctgaccattc
    35761 accgccatcg gacgatcttt gatagcgtgc atacccagaa ttgcgctctg cggcgggttg
    35821 atgatcggcg tagacatcag ggaaccgaac acaccaccgt tggtgatggt gaagttacca
    35881 ccggtcagat cttcaaccgt cagcttgcca tcacggcctt tgactgccag ctctttgatt
    35941 ttcttctcga tgtctgccat gccgagggta tcgacatcac gcagaaccgg cgtcaccagg
    36001 ccgcgcggcg tagaaaccgc catgctgacg tcgaaatagt tgtggtaaac cacgtcatcg
    36061 ccgtcgatag acgcgttcac ttccgggtaa cgtttcaggg cttcaaccac cgctttcacg
    36121 tagaaggaca taaagcccag acggatgccg tggcgttttt caaacgcttc accgtactgc
    36181 ttacgcagat ccataatcgg cttcatgttg acttcgttga acgtggtcag catggcggtg
    36241 gagtttttcg cttccagcag acgctctgcc acacgcttac gcaggcgagt catcgggaca
    36301 cgtttttcac tacgtgcagc cagagccggt tgcgccgccg gagcagccgc tgccggagca
    36361 gactctttcg ccggggcttt cgccagatgt ttttccacat cttcacgagt cagacgacca
    36421 cccacaccgg tgcctttaat ggcgctggcg tcgagattgt gttcagccag caggcgacgg
    36481 atcgccgggc ttaacgcatc gttgttttgc tcttccagag acgcctgctg gcgttgcgcc
    36541 ggagtggacg ctttctcttc agatttggcg ctggtttctt taccggcgct gttgccttca
    36601 cgcaggcgac caaggatctg acgagacgtt accgttgtac cttcatcttc cagaaccgca
    36661 tccagaatgc cgtctgctga tgccggtact tccagtacca ctttgtcagt ttcgatttct
    36721 accagcactt catcacgtac gactgcgtcg ccgggttttt tatgccaggt tgcgacggtg
    36781 gcatcggcta cggattcagg caggtcaggg accagaatat ctacgctact cattgtgtat
    36841 cctttattta ttcgacgttc agcgcgtcat taaccagatc ttgttgctgt ttctggtgaa
    36901 cggacatata ccctaccgcc ggagaggcgg aggccgggcg gcctgcataa cgcagagaag
    36961 ccccaaacgg aatcacttca cggaaatgat gctggctgca gtaccatgcg ccctggttga
    37021 gcggctcttc ctggcaccag acaaaatcct tgacgtgagc aaactgctgc aacacttcct
    37081 gcatcgcttt atgcgggaac gggtagagtt gctcgatacg cacaatggcg acatcgtgtt
    37141 gattgttctt acgacgctgt tccagcaggt cgtaataaac cttaccagaa cacatcacta
    37201 cgcgcttcac gcccttcgga tcaagttcgt cgatttcacc gatggctggc aggaaggtgc
    37261 cgttcgccag ttcttcgagg ctggaaaccg ccagcggatg acgcagcagg gatttcggcg
    37321 acatcacgac cagcggacga cgcatcccgc gcagcgcctg acgacgcagc atgtggtaaa
    37381 cctgtgccgg ggtagacggt acgcaaacct gcatgttttg ctcagcacaa agttgcagat
    37441 aacgttccag acgcgcggag gagtgctccg gcccctgccc ttcgtaaccg tgcggcagca
    37501 acatcaccag accacacatc cggccccatt tctgctcgcc agaggagatg aactggtcga
    37561 taaccacctg cgcaccgttg gcgaagtcac cgaactgcgc ttcccagatg gtcagggtgc
    37621 gtggttctgc ggtggcataa ccatattcaa acgccagcac ggcttcttct gacagtacgg
    37681 agtcccagac acggaacgcg ccctgaccgt tatggatgtg ttgcagcggc gtgtaagtgg
    37741 aaccgttaga ctggttgtgg atcaccgcgt ggcggtggaa gaaggtaccg cgaccggagt
    37801 cttcacccga caggcgaacc ggaatgcctt catcaaccag cgtggcgtaa gcgaggtttt
    37861 ccgcaccgcc ccagtcgaac agtttctcac cggctgccat cgcctggcga tcgccataaa
    37921 tcttggcaac gcgagactgc atttcaactg cttctggtac cgtactgata cgtttagcca
    37981 gttcctgcag gcgcttcatc tcaactttgt tcgggtactc ttcgtcccat tcgtggttga
    38041 ggtacggcga ccaggtgaaa gagtgcatgt tcatcggacg ccactctgcc actacgcaat
    38101 cgccagcatc cagcgcatcg cggtacaggt taaccatctc ggtggcatct tccagcgtcg
    38161 ccactttttc ctgctccagc ttgtcagcgt agattttgcg cggtgtcgga tgttttttga
    38221 ttttctgata catcagtggc tgggttgcgc tcggctcgtc ggcttcgttg tggccgtgac
    38281 ggcggtagca taccaggtcg atgaagacat cacgtttaaa ggtgttacgg aaatcgagcg
    38341 ccagacgggt cacaaaggca acggcttccg gatcatccgc gttaacgtgg aaaatcggtg
    38401 cctgaaccat cttaccgata tcagtacagt acggtgtaga acgggcatcc agcgggttag
    38461 aggtggtgaa accaacctgg ttgttgataa cgatacgtac cgtaccgcca acttcataac
    38521 cacgcgcttt cgacatgttc agagtttcct gaaccacgcc ctgcccggtc actgcggcgt
    38581 caccgtggat ggtgattggc agcactttgt tgctgctcgg ctcatcaagt ctgtccagac
    38641 gggcacgaac agagccgata acgaccgggc ttacaatctc aaggtgagac gggttaaacg
    38701 ccagcgccag gtgcaccagg ccgccatcgg tctggaagtc agacgagaag cccatgtggt
    38761 atttcacgtc gcctgtgccg aggtgttctt tatgtttacc ggcgaactcg tcgaacaagt
    38821 cttgcggttt tttacccagc acgttcacca gcacgttcag acgaccacgg tgcgccatcc
    38881 cgagaaccac ttcgcgggtg ccgctgttgc cagcgtggcg gatcatctct ttaagcatcg
    38941 ggattaacgc gtcaccgcct tccagcgaga agcgttttgc gccagggaat tttgcgccga
    39001 ggtaacgctc aaggccttca gcggcggtca gttcgcttaa gaagcgtttt ttctcttcgc
    39061 tattgaaagt cgcgcgacca gactcgatac gctgttggat ccagcgtttt tcttcggtgc
    39121 tggtgatgtg catatactcg gcaccaatcg ggccgcagta ggtttgcttg agggcggaaa
    39181 taagctcgcc gagtttcatg gtttctttgc cgctggcaaa tgaaccgacg ttgaaggttt
    39241 cctggaagtc tgcttcggtc agatcgtgga aagacggatc cagatcggcc actttatctt
    39301 gctgccacag tcccagcgga tctagattcg catgctggtg accacggaag cggtatgcgt
    39361 taatgagctg caggacttta acctgcttca cattggtgtc agggtcggag atcgttgaag
    39421 agtaacgtga agcgtctttc gccaggcggc ggaaatattc acgcgtttga gagtggaatt
    39481 gatccggttt gactcccgta ccaggtaact gctggaacgt cgaacgccag ttagcgtcaa
    39541 ccgagtcagg atcggttaag aagtcttcat agagctgttc tatccagctc tggtttgcgc
    39601 cagagaggta agaagagtcc aaccaggctt tcaaagcgct gttctgcatc gtgatccctt
    39661 aagcatcttt tttatgctta cttcgccgtg gatactacca cgcacagtga tgtgcgtgcc
    39721 ggggttcact tgcgagctct tgtctacgta gtggctcgcg aaggaacctt taaaaactgt
    39781 cttagtgtca ggggcagttt ttaaagattt cctgcatttc tttgttacgc ctgatgcgct
    39841 gcgcttatca ggcctgcggg taatgacttg taggccggat aaggcgttca cgccgcatcc
    39901 ggcactggtt gcctgatgca acgcttgcgc gtcttatcag gcctacagtt tacgcattac
    39961 gttgcaacaa catcgacttg atatggccga tggcgcgcgt cgggttcagc cccttcggac
    40021 atacactgac gcagttcatg atgctgtgac agcggaatac gctgaatgca tcgctcaaac
    40081 cgtcgaggcg gctgtcagtc tcggtatcac ggctatcgat caggaaacga tatgccgcta
    40141 acaagcctgc cgggccgata aacttatcgg gattccacca gaaagacgga caagaggttg
    40201 aacaacatgc gcagagaata cattcataca gcccgtcgag tttttcgcgc tgctctggca
    40261 tctgtaaatg ctcgcgagct ggcggatttt gtccattatt caacaggtaa ggcttaattt
    40321 tctcatattg cgcatagaat tgtcccatgt ctaccaccaa atcgcggatc accggtaaac
    40381 ctggcagcgg gcgaatcaca atcttcttgc ccggctggtt gagtgccgaa atcggggtaa
    40441 tacaggccag accattcttg ccgttcatgt tcagaccgtc ggaaccgcac acaccttcac
    40501 ggcaggagcg gcggaacgac aggctgggat ctttctcttt cagctggata agcgcatcca
    40561 gcagcatcat gtcgcgacct tcttccgctt ccagggtgta atcctgcata cgcggagcat
    40621 catcaacatc cgggttatag cgataaattg aaaactcgag tctcattttc ctgtctccgc
    40681 attagtaagt acgaatcttc ggcgggaatg ccgggcgcag tttcggttcc atgttgacgc
    40741 ttcggcgcgt catggattcc gactcaggca gatacaggga gtggcacagc cagttttcat
    40801 catcacgatc cgggaagtcg aagcggctat gtgcgccacg gctttcggta cggaagttgg
    40861 cagaaacagc cgttgcatac gccgtttcca tcaggttatc cagttccagg cactcaacgc
    40921 gctgggtgtt gaactcgctg gaagtgtcat ccagacgggc atttttcaga cgctcgcgga
    40981 tcactttcaa ctgctcaagc cctttcgcca tcgcatcacc ttcacggaag accgagaagt
    41041 tatgctgcat acattcttgc agcgctttac ggatcgccac cggatcttca ccgttacgat
    41101 tattgttcca ccggttcagg cgatccagag acgcttcaac atcagactcg ctggcatcgc
    41161 gcagtgcgcc ctgctcggcg atagactctt gcagatgcag acctgccgcg cgaccaaaga
    41221 ccaccaggtc cagcagcgag ttgccgccca gacggttagc gccgtgtacc gatacacaag
    41281 cgatttcacc aacggcaaac agtcccggaa caaccacatc ttcgcctttc tcattcacag
    41341 tcagcgcctg accggtaact ttggtcggaa taccgcccat catgtagtga caggttggga
    41401 taaccggaat cggctctttc accggatcga cgtgagcgaa ggtacgggaa agctccagga
    41461 tacccggcag acgggattcg agaacttctt tacccaggtg atcgagtttc agtttcgcgt
    41521 gtggccccca cggaccatca cagccgcgac cttcacggat ttcaatcatg atggaacgcg
    41581 ccaccacatc acggcccgcc aggtctttgg cgttcggcgc ataacgctcc ataaagcgtt
    41641 cgccatgttt gttcagcaga taaccgcctt caccacggca accttcggtg accagtacgc
    41701 ccgcaccggc aatgccggtc gggtggaatt gccacatttc catatcctgc accggtacgc
    41761 ctgcgcggat agccatgccg acaccgtcgc cagtgttaat gtgggcgtta gtggtggact
    41821 gataaatacg ccctgccccg ccagtcgcca gcacggtagc gcgggctttg aaataaacca
    41881 cttcaccggt ttcgatgcac agtgcggtac aacccaccac cgcgccatcc tggtttttca
    41941 ccagatccag cgcataccac tcggagaaaa tggtggtgtg gtttttcagg ttctgctgat
    42001 aaagcgtgtg caacagtgcg tgaccggtac ggtcagcagc cgccgcagtg cgtgccgcct
    42061 gctcgccgcc gaagtttttc gactgaccgc caaacggacg ttgatagata cgaccatcat
    42121 cgagacgcga gaacggcagg cccatgtgtt cgagttccag aatcgcttcc ggcccggttt
    42181 tacacatata ttcaatcgcg tcctggtcac cgatatagtc cgaccctttt acggtgtcgt
    42241 acatatgcca ttcccagtta tcttcatggg tattacccag cgcaacggta atgccgcctt
    42301 gcgcagaaac ggtatgggaa cgggtcggga agactttaga gagcagcgca caggtctggc
    42361 cgctctggga aatttgcagc gccgcgcgca tacctgcgcc accggcacca atcacaactg
    42421 catcaaattc tctgactggc aatttcatca caccccccac accacaacga atccataaat
    42481 cacgtaaacc accagtgcaa cgacaatcac cagttgcagc atcaggcgca aggccagcgg
    42541 tttaacgtag tcggtcaaca cctgccacat gccgatccag gcatgaatca agatagaaaa
    42601 cagcgccagc agggtgaaca ctttggtgaa cgcagaggcg aagaaaccga tccagacttc
    42661 atatgtcagt tcgccactgg tggcgaaaaa accgaccata taaatgatgt agagcgtcag
    42721 gacgatagcg gtagcgcgaa cgaggatgaa atcatgtacg ccattgcgtc ctaatgcgga
    42781 ggcgttgctt accatacgag gactcctgcg agaagtgaaa gcacgacagt aataacaaag
    42841 gagattttgg cggagcgttt acccgcttcg aatgtttctt ccagatagcc aaaatccatc
    42901 atcatgtggc gaatacctac gacgacgtga tacgccagag cggtaaggat gccccacatg
    42961 ataaatttga cgaagaagct gcccataatc gcggaagctt gctcgaaacc ttcaggggaa
    43021 gagaggctgg tacccagaag ccacagcagg atgcccactg caacaaaggt gatcacaccg
    43081 gaaacgcgat ggagaatgga cgctatcgcc gtgatgggga accggatggt ctgtaggtcc
    43141 agattaacag gtctttgttt tttcacattt cttatcatga ataacgccca catgctgttc
    43201 ttattattcc ctggggacta cgggcacaga ggttaacttt ctgttacctg gagacgtcgg
    43261 gatttccttc ctccggtctg cttgcgggtc agacagcgtc ctttccataa ctgcgcgtca
    43321 tgcaaaacac tgcttccaga tgcgaaaacg acacgttaca acgctgggtg gctcgggatt
    43381 gcagggtgtt ccggagacct ggcggcagta taggctgttc acaaaatcat tacaattaac
    43441 ctacatatag tttgtcgggt tttatcctga acagtgatcc aggtcacgat aacaacattt
    43501 atttaatttt taatcatcta atttgacaat cattcaacaa agttgttaca aacattacca
    43561 ggaaaagcat ataatgcgta aaagttatga agtcggtatt tcacctaaga ttaacttatg
    43621 taacagtgtg gaagtattga ccaattcatt cgggacagtt attagtggta gacaagttta
    43681 ataattcgga ttgctaagta cttgattcgc catttattcg tcatcaatgg attctttacc
    43741 tgcaagcgcc cagagctctg tacccaggtt ttcccctctt tcacagagcg gcgagccaaa
    43801 taacaaacgg gtaaagccag gttgatgtgc gaaggcaaat ttaagttccg gcagtcttac
    43861 gcaataaggc gctaaggaga ccttaaatgg ctgatacaaa agcaaaactc accctcaacg
    43921 gggacacagc tgttgaactg gatgtgctga aaggcacgct gggtcaagat gttattgata
    43981 tccgtactct cggttcaaaa ggtgtgttca cctttgaccc aggcttcact tcaaccgcat
    44041 cctgcgaatc taaaattact tttattgatg gtgatgaagg tattttgctg caccgcggtt
    44101 tcccgatcga tcagctggcg accgattcta actacctaga agtttgttac atcctgctga
    44161 atggtgaaaa accgactcag gaacagtatg acgaatttaa aactacggtg acccgtcata
    44221 ccatgatcca cgagcagatt acccgtctgt tccacgcttt ccgtcgcgac tcacatccaa
    44281 tggcagtcat gtgtggtatt accggcgcgc tggcggcgtt ctatcacgac tcgctggatg
    44341 ttaacaatcc tcgtcatcgt gaaattgccg cgttccgcct gctgtcgaaa atgccgacca
    44401 tggccgcgat gtgttacaag tattccattg gtcagccatt tgtttatccg cgcaacgatc
    44461 tctcctatgc cggtaacttc ctgaatatga tgttctccac gccgtgcgaa ccgtatgaag
    44521 ttaatccgat tctggaacgt gctatggacc gtattctgat cctgcacgct gaccatgaac
    44581 agaacgcctc tacctccacc gtgcgtaccg ctggctcttc gggtgcgaac ccgtttgcct
    44641 gtatcgcagc aggtattgct tcactgtggg gacctgcgca cggtggtgct aacgaagcgg
    44701 cgctgaaaat gctggaagaa attagctccg ttaaacacat tccggaattt gttcgtcgtg
    44761 cgaaagataa aaatgattct ttccgcctga tgggcttcgg tcaccgcgtg tacaaaaatt
    44821 acgacccgcg cgccaccgta atgcgtgaaa cctgccatga agttctgaaa gagctgggca
    44881 ccaaagatga cctgctggaa gtggctatgg agctggaaaa catcgcgctg aacgacccgt
    44941 actttatcga gaagaaactg tacccgaacg tcgatttcta ctctggtatc atcctgaaag
    45001 cgatgggtat tccgtcttcc atgttcaccg tcattttcgc aatggcacgt accgttggct
    45061 ggatcgccca ctggagcgaa atgcacagtt acggtatgaa gattgcccgt ccgcgtcagc
    45121 tgtatacagg atatgaaaaa cgcgacttta aaagcgatat caagcgttaa tggttgattg
    45181 ctaagttgta aatattttaa cccgccgttc aaatggcggg ttaattttta tatgcctaaa
    45241 cacaaaaaat tgtaaaaata aaatccacta tcggacattc atatattcta aaaaaaaata
    45301 tatcggtata aattataaac aaatcgatga tttttaatca tctatcttcc tctcagttac
    45361 atgaataaac caccaaatat agcgcacccc tataatttcc aatactttat tcatcaaaca
    45421 tttaattcat accatcaatt aattactttt acatttaata aaattaataa tatttttaag
    45481 aaaaactaaa aacattcgac aatcaaataa gatattcttg caccgatata cttttttctt
    45541 ttctgttaaa ggactcagtt atgtttaaag gacaaaaaac attggccgca ctggccgtat
    45601 ctctgctgtt cacagcacct gtttatgcgg ctgatgaagg atccggtgaa attcacttta
    45661 aaggtgaagt tattgaagca ccgtgtgaaa tacatcagga tgatattgat aaagaggttg
    45721 aactcggtca ggtgaccacc agccacatta atcagtcaca tcacagcgat gccgttgctg
    45781 tcgacctgct cttagtcaac tgtgatctgg aaaactccag caacggttcc ggtggcaaga
    45841 tttctaaagt tgcagtcacc tttgatagct cagcgaaaac caccggcgca gatccgattc
    45901 tcaacaacac cagcacgggt gaagccacct gcgtcggcgt acgtttaatg aataaagatc
    45961 aaagcaatat cgttttaggc accgctactc cagatatcga cctggctccg acctccagcg
    46021 aacaaacgct gaatttcttt gcctggatgg aacaaattga tcaggctaca cctgtaacgc
    46081 caggcgcagt tacggcgaat gcaacctacg tgctggatta taaataagta ttactttggc
    46141 ggcgctatcg ccgcctttca taaaatattt atcaggtatg gacaccgtga atatttatcg
    46201 actctctttt gtatcctgcc tggtcgtggc gatgccttgc gcattggcgc tcgaattcaa
    46261 tctgaatgtt ctcgataaat caatgcgcga ccgcattgat atttcattat taaaggaaaa
    46321 aggagtcatt gctcccggtg agtattttgt tagcgttgcg gtaaataata accaaatcag
    46381 taacgagcaa aagattaact ggcacaaaaa tgacgataaa accattccgt gcatcaatga
    46441 tttactggtc gataaatttg gcttaaaacc tgaagtccgt cagtcattac cattgataaa
    46501 tcagtgcgtc gattttagct cccgacctga aatgctcttc aatttcgatc aagccaatca
    46561 gcaactgaat attagtattc cgcaagcctg gctggcgtgg tactcagaaa actggacccc
    46621 accctccaca tggaaagaag gtgtcgccgg tgtcctgatg gattacaact tgtttgccag
    46681 cagctaccgc ccacaggatg gcagcagcag cactaatctt aacgcctatg gaacagccgg
    46741 aatcaacacg ggggcatggc gcttacgcag tgattatcag ttgaatcata ctgatagcga
    46801 tgataaccat gaacagtcag gcgaaatatc acgtacctat ctttttcgcc cgttaccgca
    46861 attaggttca aaattaaccc tcggcgaaac ggattttagt tccaatattt tcgacggttt
    46921 ttcttatacc ggcgcggcac tggcaagtga cgatcgaatg ttgccatggg aactacgcgg
    46981 ctacgcccca caaattagcg gtattgcaca gaccaatgcc acggtgacga tcagtcaatc
    47041 aggccgcgtc atttaccaga aaaaagtccc accaggccca tttatcattg acgaccttaa
    47101 tcagtctgtt cagggcacac tggatgtcaa agtgacggaa gaagatggtc gggtgaacaa
    47161 tttccaggtt tcagcagcat cgacgccctt cctgactcgt cagggacagg ttcgctataa
    47221 actggccgcg ggtcagccac ggccctccat gtcacatcaa actgaaaatg aaaccttttt
    47281 tagcaatgaa gtttcctggg ggatgctgtc aaacacctcg ctgtacagcg gcctgctgct
    47341 ttctggtgat gactaccatt ctgccgcaat gggtattggg caaaatatgc tgtggcttgg
    47401 tgcgctgtcg tttgatgtca cgtgggccag tagccatttt gatactcagc aggacgagcg
    47461 gggcttaagc taccgtttta attacagcaa acaagtggat gccactaaca gcacgatttc
    47521 cctcgccgct tatcgtttct ccgatcgtca ttttcacagc tacgccaact atctggatca
    47581 caaatacaac gacagcgatg cgcaggacga aaaacagacg atcagcttat ctgtgggcca
    47641 accgattacc ccactaaacc tcaatcttta cgccaacctg ctacatcaaa cctggtggaa
    47701 tgcagacgcc tccacgaccg ccaacatcac ggcaggtttt aatgttgata ttggtgactg
    47761 gagagatatc tctatttcta cgtcattcaa tacaacccat tacgaagata aagatcgcga
    47821 caaccagatt tacctgtcga tttcgctccc cttcggtaac ggtggtcagg ttggctatga
    47881 catgcaaaac agtagccaca gcaccacaca ccgcatgtcg tggaacgata cgctggatga
    47941 acgtaatagc tggggcatgt ctgccggact gcaatccgac cgtcctgaca atggagccca
    48001 ggtgagcggt aactatcagc acctgagttc agcgggtgag tgggatattt ctggtaccta
    48061 tgccgccaat gattacagtt ccgtcagcag cagctggagc ggttctttca ccgcaaccca
    48121 atatggtgca gcgtttcatc gccgcagctc caccaatgaa ccacgcctga tggtcagcac
    48181 cgatggcgtg gcagatattc cggttcaggg caatctcgac tacaccaacc attttggcat
    48241 tgcggtggtg ccgttgattt ccagttacca gccttccacc gtggcggtga acatgaatga
    48301 cttacccgac ggcgtaacag ttgcagaaaa cgttatcaaa gaaacgtgga ttgaaggcgc
    48361 gataggttac aaatcactgg cttcccgttc cggtaaagac gttaacgtca tcattcgcaa
    48421 cgccagcggt cagtttcctc ccctcggagc ggatatccgc caggatgaca gcggcattag
    48481 cgtggggatg gttggcgagg aaggacatgc ctggttaagc ggagtcgctg aaaatcaaaa
    48541 gtttaccgtg gtctggggtg atagccagca ttgctcgctc catcttcctg aacatatgga
    48601 agacaccgca aatcgcctga ttttaccttg tcattaatta aggaaatgaa aatgacattt
    48661 atgaaaggac tacccctttt gttgttggtt gccagtctgt gcagccacgc tgcactacaa
    48721 cccgatcgca ctcgtattgt gtttaacgct aatgataaag ctaccagtct gcgggtggat
    48781 aatcgtagcg ataaactccc ctacctcgct tattcctggc tcgaaaatga aaaaggggag
    48841 aaaagcgatg atcttctggt cgccctgccg ccgatacaac gtctggaacc gaaagcaaca
    48901 acgcaggtac ggatagtaaa gcaagcctcg accacaaaat taccgggcga tcgcgaaacg
    48961 ctatttttct acaatatgcg tgaaattcct cccgccccgg aaaaaaacag cgaccatgcc
    49021 gttctgcaag tcgccattca gagtcgtatc aaggtgttct ggcgaccggc tgcattacgc
    49081 aagaaagcag gagaaaaggt cgaactgcag ttacaggtca gccagcaggg caaccaactg
    49141 acactgaaaa atcctaccgc gtattatctg accattgcct acctgggacg aaatgaaaaa
    49201 ggcgttctcc ccggcttcaa aaccgtgatg gttgcgcctt ttagtacggt caacacaaac
    49261 accggaaatt atagcggcag ccagttttat ctcggttata tggatgatta cggcgcgtta
    49321 cgcatgacga cgctcaactg cagcggacaa tgccatttac aagccgtgga ggcgaagaaa
    49381 tgagtgctgg caagggagtg ttgctcgtca tctgtctgtt atttctgccg ttgaagtctg
    49441 cgcttgccct gaactgctac tttggttcat ccggtggctc ggtagaaaaa tcagaagcga
    49501 ttcaaccttt tgctgtacca ggcaatgcca aactcggcga taagatctgg gaatctgacg
    49561 atattaaaat tcccgtctat tgtgacaaca ataccaacgg caactttgaa agcgaacatg
    49621 tctacgcatg ggtaaacccc tacccggggg tgcaggatcg ttattatcaa ctcggagtaa
    49681 cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac actaaccagt
    49741 gtatcgacag taagaacatt gacatttata cccttgagca gatcatcgcg atgggatggc
    49801 agaacaaaat ttgctcgggt gatcccgcta atattcacat gtcgcgtacc tttctcgctc
    49861 gcatgcggtt atacgtcaaa atacgagaaa tgccgccgca tgattatcaa agtacgctta
    49921 gcgactatat cgtcgtgcaa tttgacggtg ccggtagcgt taacgaagac cctactgccc
    49981 aaaacctgaa atatcatatt actggtctgg aaaacattcg cgtgctggat tgcagcgtca
    50041 atttttccat ttcaccggaa acacaggtga ttgattttgg taaatttaat ttgctggata
    50101 ttcgtcgcca cacaatgtcg aaaacattca gtattaagac gacaaaatca caaaatgacc
    50161 aatgcaccga cggatttaaa gtcagttcct ccttttatac cgaagaaacg ttggttgaag
    50221 aagataaagc attgttgatt ggtaatggtc tgaagctacg tttgctggat gaaaacgcct
    50281 cgccttatac cttcaataaa tatgctgaat acgccgattt caccagcgac atgttggtct
    50341 acgaaaaaac ctatacagct gaactttcgt ccattgcagg tacccccatc gaggctgggg
    50401 cccttcgata cagtggtgct ttttaaaatt aactataact gatttgccat gatgcccttt
    50461 gcagccggaa agggcatctc tttcagaaaa ttgctgattt tccccttttc cttgattgct
    50521 gttatttttt cctagcccgg ctttctttat gctgtatcga tacaccaaag agaaaataat
    50581 gagggagcga aggatgccag ttttgcagtg gggaatgtta tgtgtgctat cacttctcct
    50641 ttctattggt ttcctcgcgg tacatctccc ggcggcgcta ttacttgggc cgatgatcgc
    50701 cgggatcatc ttcagtatgc gcggaattac tctgcaactt ccccgctccg cttttctcgc
    50761 cgcgcaggcc attcttggct gcatgattgc gcaaaacctc actggttcga ttctcaccac
    50821 gctggccgcc aactggccga tcgtgcttgc tattttactg gtgacgctgc tttccagcgc
    50881 catcgtgggt tggttattgg tgcgctatag ctcactgcct ggaaataccg gtgcctgggg
    50941 ttcttctcca ggcggcgcag cggcaatggt cgctatggcg caagattacg gcgcagatat
    51001 tcgcctggtg gcgtttatgc aatatctgcg agtgctgttc gtcgcgggtg ccgctgttct
    51061 ggtcacccgc atgatgctgg gtgataacgc tgaagcggtt aaccagcaaa tcgtctggtt
    51121 cccgccagtg agcattaatc tcctgcttac gattttgctc gcagtcgttg ccggtacggc
    51181 gggatgcatg ctgcgtcttc cttccggcac gatgctcatc ccgatgctgg cgggcgcagt
    51241 gctccagtct ggtcagctca tcaccatcga actaccggaa tggctgctgg cgatggcgta
    51301 tatggcaatt ggctggcgga ttggtcttgg tttcgataaa caaatcttac tgcgggcact
    51361 acgcccgcta ccgcaaatcc tgctgtcgat ttttgctctg ctggctattt gtgcaggtat
    51421 ggcgtgggga ctgacccggt ttatgcatat tgattttatg actgcctacc tcgccaccag
    51481 tcccggcggg cttgataccg tggcggtcat cgccgcaggg agcaatgccg atatggcgct
    51541 tattatggcg atgcaaaccc tgcgcctgtt cagtattttg ctgacagggc cagccattgc
    51601 gcggtttatt tcaacctatg cgccgaagcg gtcggcctag tgctggcagc ctggacacca
    51661 gtaaaacggg cgcgaggaca acgtggtttt ctcaatgatg ccgccacagc gttcgcacag
    51721 ttcgccatca cgatgaaaaa ccttaaagcg aaacagcgcc ccatgatgct tattctcatc
    51781 cacctgcccc cgcgtagcgt aagaaaaccg aggaatctcc agtaacgcgt gtgcgagtgc
    51841 atccagttgc gccgcattga gatctttcgc tttatgattt ccagtcaacc caacctgcca
    51901 gaggatctcc acccgcaaat aattgccaag cccagccaga aacgcctgat cgagcagtaa
    51961 tcctgcaaac tgacggttac gaaagcgcgg cgacaataac cgttccttta ccacctccgg
    52021 cgtcagattc ggatccagca catcgggacc aacgcgttgt aaaaacggat gcgtggtcag
    52081 ttgttccggc ctcaacatct caatatccga ggcgctataa agcagaatgg ttttgtcagc
    52141 cgtttgcagt tttacccgca atactcgcgt ggtctgcggc tcttcgccgg tatcaaccac
    52201 gcgccagacg ccgtaaagct gattatggct gtagagcgtt aaaccgttgg gaaaatgagt
    52261 taacaacgcc ttaccacgcg tttccacatg ggtaacgtgt tgaccgataa gttgtgattg
    52321 ataagttttt aactgcggga aggcaaacca gacatcagtt agtggtttgc ctttgatcgc
    52381 cgcctccagg ttatccgctg cacggcggat ctccgggcct tcaggcatgg tgatatcctt
    52441 ttttcattct ttattaagcg gggttatgct gcgacaacaa tccccttttt ggcaaatgta
    52501 gctcgtaatc ggcgggcgaa cgccagagcg tgctcgccgt cgccatgcag gcagaccgtt
    52561 tgcgccgcga ccgttgccca ttcgccggtg atacttttga ctctgccgtg ttgcaccatc
    52621 tccagcgttt gcgccaacgc ctgttcttcg ttttcaatca acgcgcctgg ctgacttcgc
    52681 ggcaccagcg agccgtcagc ctgataaccg cgatcggcaa acacttcctc gcgcgttgtc
    52741 aggccatatt gcttgcctgc acgaatcagc tcgcttcctg ccagcccaac gagaaccaat
    52801 gccggatcgc aagcgtatac cgctctggcg atggcgtctg ccagttgtgc ttctttcgcc
    52861 gcctggttgt acaacatgcc gtgcggtttg acatgacgca ttacgccgcc ttgcgcacgg
    52921 gcaatcgttg ccagcgcgcc aatttgatac agcgtctggg cgtaaacggt ttccggcggc
    52981 agctgcatgg cgctgcgacc aaaattttcc ctgtcgggaa aactcgggtg agcgccaatc
    53041 gcgacaccat tttttattgc ttcacgcacg caagcctgca tggtttgcgc atcgcctgca
    53101 tgaaatccac aagcaatatt ggcagaggaa accagcgtta atagctctgc gtcgctggcg
    53161 cagccttcgc ccagatcggc gttcaggtca attttcattg tgcagccgcc acgctaattg
    53221 ttcaaaataa cgttgctgat cttgccgcgc tttcagtgcc tcttccagtg aacactggac
    53281 aaaatgaatc ggctgaccga gcggaatttg cgccagatgg tacatatcag cctcaatgat
    53341 acaggcaata cgtggataac caccggtggt ctgtgcgtcg ttcatcaaca caatcggctg
    53401 cccgttatgc ggcacctgca ccacgcccgg taacaaaccg tgagataaca gttcgcgatc
    53461 ggtgatgcgt tttaaaattt gcccctgtaa acgatagccc attcggttac tttgcgagct
    53521 aagctgccag ggcgaacgcc agaatgcatc ctgcgaggcg cgatcgaact catgatattc
    53581 cggccccggc aaggcgcgaa tgcggttgcc ccacagcagc tgtttaacgc cctgcgcttc
    53641 cataaaatca cgcttcgatt tgccaatcgg gagtcggtca ccatccttca gtaaacggcc
    53701 ttccagcccg ccaatcccca ctttgagatc ggtgctgcat gaccccatta ccggcggaac
    53761 atcaatacca cccgcgaccg ccagataact gcgcatcccg tgctgcgggc gtttaagcgt
    53821 taaacgctgg cctgctttca tcggcaatcg ccagccggtc cagacggcgt tatcatccaa
    53881 ccgcgcttcg caacctgcac ccgtcagggc aaaccaccca tcagtttcga actcaactgt
    53941 taactgaccg agcgtgatct ccagcgcggg ggcattggcg tcattaccca ccagcaggtt
    54001 agcaatgcgc agcgcaggca tatccagtgc gccacagtgg ctgatacccg actggcgaaa
    54061 accgtgacga ccgccatcct gcacagtggt gtacatgccc gcacgaataa tcttcagcat
    54121 attccctcct tctgcggtac aaatcgcacg ctgtctcccg gacgtaataa gatgggttcg
    54181 tcacgcgccg gatcaaacag gctgagtgag gtatgaccaa tcaactgcca gccacccggc
    54241 gttgccagcg gataaacacc agtctgcggc ccgccgatcc cgacagaacc tgccggaacg
    54301 agcaagcgcg gttcagcgcg ccgtggcgtg tgttgttgtt ccggcaaact cccgagatac
    54361 gggaagcccg gttgaaaacc taaaaaccag accacgtatt ccacggagga gtgcaattca
    54421 acaacctgtt tttcgctcaa cccgcaatac gccgcgacca ccgccaaatc cggtccgcct
    54481 gcaccaccgt aaaccaccgg aatttcaata aagcgagact caggttccag ggcctcgctc
    54541 tcctcccacc agcgttgcaa acgctctatg gcatccagcg ccagcgactc aggattacgc
    54601 aaaatcaccg tgatattgtt catgccggga atggcttcaa ccacattcgg catatccacc
    54661 agacgctgcg ccagtcgcca gatccgcttc tggctagcca gcgtcaccgg cggttccagt
    54721 tccagcacta ccgccgtttc acctatcaga taacaacgcg ctcgttgcac tttcgatccc
    54781 tctgattatt cgttatgcag gattaggaat atcaataaag gtcacatcaa gatcggtatt
    54841 ttcattcagc cactcgctca atgcgcgaat accaccgcgt tcagtggcat ggtgacctgc
    54901 agcataaaaa tgcaatccct gctcgcgggc tgaatgaatg gtctgttcag aaacttcgcc
    54961 agtaataaaa gcatccacgc caaaacgcgc ggcgctatcg ataaaacttt gcccgccgcc
    55021 cgtgcaccag gcgacgcgct ggaccacctc aggtccggta tcgccacacc ataacggctt
    55081 acgtcccaga cgcgcttcaa tccaggaggc tagttccatt cccggcacag gcatggtcag
    55141 ctctccccac ggcaccaacg gctcaatttc gcccatgacc gtgatcccca gtaacgccgc
    55201 cagctgtgcg ttattgccca gctcaggatg tgcgtcaagc ggcaaatgcc agccatacag
    55261 gttgatatca ttcgccagca acgtttttaa acggttacgc ttcattccgc gaatgaccgg
    55321 agactctcct ttccagaagt agccatgatg cacaatgacc gcatccgcgc ccagacgcac
    55381 tgcctcatcg agcaaagcct ggctggcggt gacaccggta acaatttttt gcaccgtctc
    55441 tttgccttcc acctgcaaac cattcggcgc gtaatcacta atcgccgcgc tgttcagttt
    55501 ttcgttaatc agttgttcca gttcggtgtt tttcatcttc gcgccttccg tattctgata
    55561 gctacaccgt taacatagcg ttacctcgcc tgttcgtcga tagccaatct tttgacatat
    55621 taagaaacaa acccgacgcc aaaagacgca aattgtttat atcatcacgc ttcagctaat
    55681 gtatatttat gcaatcagac agcatattgc ataaataatc agttctgtat caaagtttta
    55741 actttaagga aacacaagca cgccgcgcaa ctattacgtg acgttgccgt caattgtgga
    55801 attattatga ataaacacgc atcacaaccg cgcgctattt actatgtcgt tgcgctgcaa
    55861 atctgggaat attttagctt ttacggcatg cgtgccctgc tgattctcta tctcaccaat
    55921 caactaaaat acaacgatac tcacgcctac gagttattta gcgcctactg ttcgctggtg
    55981 tatgtcacgc caatcctcgg tggctttttg gcggataaag ttctcggcaa tcgcatggcg
    56041 gtgatgctgg gggcgttgtt gatggcgatc ggtcatgtgg tgctgggtgc cagtgagatc
    56101 catccgtcat tcctctatct gtccctggcg attatcgtct gcggctatgg cctgtttaaa
    56161 tctaacgtaa gctgtctgct cggcgagctg tatgagccaa ccgatccgcg tcgtgatggc
    56221 ggtttctcgc tgatgtatgc ggcgggtaac gtggggtcta ttatcgcacc tatcgcctgt
    56281 ggttacgccc aggaagagta cagctgggcg atgggctttg gcctggcggc ggttggcatg
    56341 atcgcgggtc tggtcatttt cttatgtggc aatcgtcatt tcactcatac ccgcggcgtt
    56401 aacaaaaaag tactgcgtgc gacaaacttt ctcctgccga actggggatg gctgctggtt
    56461 ctgctggtgg caacgcctgc actgattacc gtgctgttct ggaaagagtg gtcggtatac
    56521 gccttaattg tcgcgactat tattggcctg ggtgtactgg caaaaattta tcgcaaagca
    56581 gaaaaccaga aacagcggaa ggagctgggg ctgattgtga cgctcacctt attcagtatg
    56641 ttgttctggg ccttcgcaca acagggcggt agctcgatta gcctttatat cgaccgcttc
    56701 gttaaccgcg atatgtttgg ttataccgtt ccgaccgcga tgttccagtc gattaatgcc
    56761 ttcgcagtta tgctgtgcgg tgtgttcctg gcgtgggtgg ttaaagaaag cgtcgcaggt
    56821 aatcgtaccg tgcgcatctg gggaaaattt gctcttggcc ttggcctgat gagcgccgga
    56881 ttctgcattc tgaccttaag cgcccgctgg tccgcaatgt atggtcactc ttctctgcca
    56941 ctgatggtgt taggcctcgc ggtgatgggc tttgcggaac tgtttatcga cccggttgcc
    57001 atgtcgcaaa ttacgcgcat tgaaattccg ggcgtgaccg gcgtattaac cggcatctac
    57061 atgctgcttt ctggcgcgat cgcgaactat ctggcgggcg tgattgccga tcagacatcg
    57121 caggcttcgt ttgatgcttc cggggcgatc aactactcca tcaatgcata tattgaagta
    57181 tttgatcaaa ttacctgggg cgcactggcg tgtgtaggac tggtactgat gatttggctg
    57241 tatcaggcgc tgaaattcag aaaccgcgcg ctggcgctgg agtcataata ggccagcacc
    57301 gatgcctgat gcgacgcttg acgcgtctta tcaggcctac gtatttcctg caatttattg
    57361 aatttgcaca aatttgtagg ctggattagg cgttcacgcc gcatccggca tctgggctcg
    57421 attgcctgac gcgtctgtta tttccccttc cgcgccgtct catacgctgc caacgtttgt
    57481 actctcgcct ctttgtgctc gactatcggt tgcggataat ccagcttcac acctgctttc
    57541 tgcgcccact tccacggctc atgcaccgct ttccctggta catcgcgcag ctccggtaac
    57601 catcgacgaa taaactcgcc ctcacggtca aatttctcgc cctgagttat aggattgaaa
    57661 atacgaaaat acggcgcagc atcagtaccc gtagaagccg cccactgcca gccaccgtta
    57721 ttagctgcca aatcaccatc aatcagctgc gacataaaat atcgctcgcc ttcgcgccag
    57781 tcgatcaaca aatctttcac cagaaaactg gctgtaatca tccgtagccg gttatgcatc
    57841 cagccagtgc tgttaagctg acgcatagcg gcatcaataa tcgggtatcc cgttttgcct
    57901 ttctgccagg cctgtaaatg tgcgggattg ctttgccact gcacacgatc cgtccaggca
    57961 ataaatggac gatgtttaca caacgagggg taatacgtca tcagatgacg gtagaactcg
    58021 cgccagatca gctcactaag ccagacacta ccggccccac cgtccagcgc ctgcggctgt
    58081 tccgccaaca agcgatgcaa acactggcga ggcgataacc cgcccgttgc caagctggcg
    58141 gacaaacggc tggtgccttc cactgccgga aaatctcgtt gttgctcata ctctccagca
    58201 ccgttctcgc aaaactggcg taattgcgca atcgccgctt tttcttccac cgggaaatgc
    58261 gcagtatcga aagactgacg aggataattc agcgtaatgg atggcgcggg ctttatcgat
    58321 ccgctactac gaacttttgg tgcagcgacg cactccggca tcccttcccg cagccgtttc
    58381 agccaggcat tcttaaaagg cgtaaagact ttgtacatct cgtgattgcc ggtcatcacc
    58441 gcccccggcg gcaggatcac gctgtcatca aatccttcac acaccacgtt acgcagcgct
    58501 ctttcaacgt ccacatcccg cgctcgctca ttcacctcat actgatagtt ataaaaaaga
    58561 tgagtaacgc tgttttccgc gcacacctgt ttaactattt cgacactggc gacaaagtca
    58621 tccacttcac ggaacaataa aggaatacct ttttccgcca gcgcgatttg tagcccattc
    58681 agttgagcat tgataagttc agcctgacgc ggcgacatgt tatgcgtctc ccactggcgt
    58741 ggtgtagcga tatacagcgc cagcacgcgt gcagacgaat tgcggcaggc ggcagccagt
    58801 gcgagattat cgtgcagacg taaatcctgg cgaaaccagg ccagatgggt agtcataaaa
    58861 ctcctgaatg gcgcaagtta atcacacgcg gccaaagttc gaaataacgc tgccctgaaa
    58921 gccaggcgtc aggataaagc gccatatagt gtttgatacg catcagcggc gcaagaagtg
    58981 gttgctcgcc acggcgataa ctgtcgataa gcgcagccag ctgctggcgt tctgtggaat
    59041 caatatgcgg gcgaaaataa ccctgaacgt gcatcaaaac gttggtgtga tcgcgcgggt
    59101 tggcgggatg agaaagcaaa acaattacgc gttggcggta ctggttgtaa aaatcatcca
    59161 gattttgcca ctgatgaata tcagccacaa acgggccaag tttgcggtac tcgggctgag
    59221 aatgggccag aaagacgagt ttatagcggc tgtgataatc gagaagcgcc ccgcgcgtta
    59281 agccatgttc tttcagtcga tgtaactcat gaagggcgaa gatacggatt agggtgctgt
    59341 catcaaatcg ttgtagattc attactgctc ctgaacggta tacaggagta agtgtagacg
    59401 ttggtaaatg atgtttgtga agttaatccg ctagcagggc aagattacga aagcgcgctc
    59461 cccgcaagca actaatcaag gtagttaatc atggctgaac acgatattac gccagaagtc
    59521 accatttcca aaacacagcg ccgctataaa gtcggctatg tcagagcccg ccaccaggac
    59581 cgcaaaaccg gtatagcccg gtattatagc ctgcacctta agggcgactg gctgaaagag
    59641 gccggatttg ataccggacg gggtgtcacg gtgaagattt cggagggggg cctgaccatc
    59701 atcgctgaca gtgacgaggt gcaggaactg cgggaggaac tttatcaggt taaacaggta
    59761 gttaagggga tgcgggatgg gatgtttagt gtgctgaacg aaagttaaat attaaaaaag
    59821 ctgggaagat ttaaaacctt cctagctttt tattctgtct tattataatg tatactcatc
    59881 atcttccgat gaatttataa attgctccaa gtttacatga agagtactta acctagtttt
    59941 taactcatta agtgaagcga taatttcata ttttccaaca taatcatatg atggatcgta
    60001 ttcattatct atgtcagaag ctaaaaatga tgatattcgt agtttaaatt tagcttcatc
    60061 tttattacat tcattggcat aaggtgaatg ccctatcata gcatattcct ctaatctaaa
    60121 aggataagat ggttttaatt catcgatcaa tgaaatttca atccatgttc tatatccagg
    60181 cattaatgga ctaatttctt tgagcaaaac ctttccactt aataatgtat tgagattgtt
    60241 cattattatg tctccctcgg gaaaaatata tgtctaacac ttccaccatg ttgatgaact
    60301 tgtaaatgtg gtaaatcacc atcaggattt cctggtccat gacctgacac tctatatgga
    60361 ggttttgtta cagaaggatc ttttatatca tcccaatgat atgaacctgc ttttgtttta
    60421 tttccagtaa gatattccat taagttctta gtgctggggc ctgactcacc tgtcatattt
    60481 ttataattat gacctaaata tctatccata aacaattgtt cagcgtcact tctagaccta
    60541 acagtaactt tggtttcact actatctaat aaacgattcg ctttttggtt actaacagca
    60601 catggagtta atccaagtgg atcagcatat tttagaggat taggtgcata actgtagaga
    60661 tttaaaccgc ctctcaaccc tatcgggtca gttaccatga accgcccgac atccggatca
    60721 tagtacctgt gcagattgta gtgcagcccc gtttccctgt cgaggtactg tccctggtag
    60781 cgcaggttct gctccacccc gccgtggtcc ttctcctgta tcgtgttacc ccagacctga
    60841 taccccgtct cccacacgat tttcccgtcg ctgtccgtca tctcttccgg cgcgccgttc
    60901 acatccgtgt gaaaatacag tatccggttc gggccctctt tgcccgcctt atccacccgc
    60961 gccagcggtt cataactccc ctggtccctg tagatgtaca ggctgcctgt tccctccggc
    61021 ctgctctcct gcgccagccg cattccgtcc cataaaaatt ttgtccggtt gtacggctta
    61081 ccgtcagggc tgatgtgctg tttttccgtc cgcctcccca gtgcatcgta cacataccgg
    61141 tatgtccccc gcgctgtcgt cacttccacc atccggtgtt ccgcatcata acggtaactc
    61201 tgcgttccgc tccggccctc cttttccacc gcacggccaa attcgtcata acggtagtgc
    61261 acaccacggt aagacatcag ccggttgaac ggcaccacgc tgttttctgc cgtggccctt
    61321 tcccccgcgc gcctccccag caggtttccc gccgcgtcgt actgccagct cgccagatac
    61381 ccctcatcct ccgaacgaca cgccgtgatg ctccccgtgg ggtcgtagaa gtaatccgtt
    61441 tccccccgcc ggctgtgccg cgtcttcagc agttcatcct cgccactgta gctgtactgc
    61501 cggtccgtga acgtctccgg taccaccccg cgcatcccgc tgtatgtctc cctgcgcttc
    61561 agccgcccgc cggggtcgta aagctgccgc gtgctcagtc gcccctggct tctgagcact
    61621 tcacggtgca ggtggtcgcg cgtgaactcg ctgatgacct cccggtccag atttatctgc
    61681 tggagatggc cgctgccgta gtacagatgg cgctgcgttc tgccgtccgg aaaggtggtg
    61741 gcagtccggt tgcccagttc atcgtactgg tgggtcagga tgccctgcgc cgtctcctcg
    61801 ctcagcatcc gccccagtct gtcgtagctg aagcggataa cttccggcgc gtcagtttcg
    61861 tgccgctggc gacggatttc cagtagctgg tctgccgcat catggacgta ttccgtacgg
    61921 ccatcggctg tctcttttgc cgtgagccgg cccgccgcat cacgcgccag ccggtggcgg
    61981 atttcaccgc cccggtcccc ggtgaacacc acttccgtga ccgcgttcag cgcgttgtag
    62041 cggtacgact ggcagcagcc gtccgggttt atctgctccg ccagacggtc cagcacatca
    62101 tggcggaacc agtagcgttc tccgttctcg ttcgtgagcg tgaccagacg cccgtaagcg
    62161 tcgtacccga atgccgtcct gcgccccatc gcgtccgtcc ggctgcgcac ccggccctgc
    62221 ccgtcccgct gccagcgcgt tatcctcccc atcgggctgg tgtactttac cagcctgccc
    62281 gccgcgtctg actggtaatt caccgtgcgg ccgtccgcca gtatcacctc cgtcagatga
    62341 ccgctgtcgt catacccgta gcgcgtgctg tggctctgcg catccgtcat tctgataagc
    62401 ctcgtgcgtt cgtcgtagaa ccacgccgtc tgcgagccgg aacagtccgt gtgccgcatg
    62461 agctgcccgt cttcattcca ctggaggtat ttattccccc cgcgcgcatc tgttatctgc
    62521 accacctggc cgtggtggtc atactgatac cgggtgttct gctgcagcgg gtcggtcacg
    62581 gcaagaaggt tgccgcgttc atcgtacaca cagcgccagg tgtttcccgc cgcgtccacc
    62641 tccgtttccg gctggtgcca caccgggtgc cactgcgcct gctccacccg ccccagcggg
    62701 tcgtgcgtct ccgtgatatg cccctgcagg tcatacacat agcgccattt gccgccctga
    62761 gggtcggtca tccccagcaa cagtcgttct tcgtcgctcc agcggaatgt cgtcacctgt
    62821 ccggcttcgt cctggtacgc cgtcacctga ttctgcgcat cccactggtg ataacgtgtg
    62881 cgccccagtc cgtccgtcac gcacagcctg cgctgcgcca ggtcccacgc cagccggtac
    62941 tgttcaccgc tgtccgtgtg gtgccccgtc acccgccatg ccgcgtcatc cggaccttca
    63001 taccagcccc actggtaata atacccttcg ccgcccggct ggcggtgcag cgtcatgcag
    63061 tggttttcgt cgtaagcaaa ctcacgcagc aggtgccccg tgctgtcgta cacgccgttc
    63121 aggtggccgg cctcgtcata gctgtaacga cgcagcagta aaggtgcagt ctcagggaaa
    63181 tgctgataaa tctgtgtcac ccgccgggga tgcgccgcca gttcgtaata cagacggatg
    63241 cacggacgct gctgttcgcc gctgatttcc acaatgcggc cccggtcgtc gtagtacaga
    63301 ttcagacagt tgctgttacg gtcgcccagc attttcagcc gctggcgctg cgggtggtgc
    63361 gggtcagcct caaacagaaa acactgcccg tcatcgctgc tgataagcca gtgcccctgc
    63421 tcaccacgcc ttaccgccag cccttcaccg gggctgtaaa acccgccccc cggcagtacg
    63481 gcttccatat ccaggcgtcg ccccatgccc gacacaaagg tcatgcagtt ttcgtctgcg
    63541 ctacccggtg tgcacacgag gctgacttca tacattacac tccagccctg accaaacatt
    63601 ccttccgtac gttcgtcacg actgctgtac acgcgctgcc agtccagcgg aaagtggccc
    63661 ggcagactga aatcgaagtc atcgtcatcg gccaggtatt tggccccggt ggcggcattg
    63721 accggatgcg gaagggcact ggtggccttc tgcgtcagca tgctcatccc gacactggca
    63781 gcaaaacagg cgattttaga aagcattttg cccgggcgaa gaaacatcag cgccacggtt
    63841 gccaccaggg ttatctgcga cctgccgctt ctgatatccc tgaccaccag taacggaccg
    63901 ccaatgcgca catccggtga cacaaacgcc ccgttccccg gctcattcac caccctggcc
    63961 tcgcaggtac agcgtgcccc gctgcgtgtc gccggctggc tgttgatacg cacatcccgc
    64021 gcgccttccg cgatatattc cgctgcgggc ggctcgtcat cgcctttgaa cagattactg
    64081 atgctgccaa taaccgcatt cactccctga aaggcggttt ccagcatacc gcccggattc
    64141 agcaggttaa gggccgttcc cagggggtca tccagaaagg cggctttttt ttgtgcgaga
    64201 aactgcgtca gtgaggaagg atgtttatgg cagtcgattt tatcgtcagg ggtcggggtg
    64261 gagcccgggg ccgcactggc cacagtgggc tgccatagtt ccgacacaaa atgtgtcacg
    64321 ccatccacca tttcagaacg cccttcttcc gtactgagct ggtcgtgcag agcccgggac
    64381 atcagcacag gccctgtcac cgggttaacc gccagggcta tggcccccgc catttccaga
    64441 tattccccgg ccacatcccg gaacgttctg gcatcctcct gacgctgcgc ttcttcctgt
    64501 tctgccttcg cctgcgcttc ctgcgcctct atgtcctgcc gcgatgccgc agtcgccgct
    64561 gcccgcgcgg caggtttgct gttgatccat gtgtcaccag agccggtgag gatgtaacct
    64621 tcaatctgag gcgggaaaag catatttgcg agggctgaac aggcttcgct gaccttttct
    64681 gtcacgccgg tgtacaccat cgccacaccc gccacaaaac cggtcagcac gacggccgtg
    64741 ccccccatcg tgatccccac tgacgtcgcc agtaacgagg ttgtgccgac aatgagggca
    64801 tcaataacga catttgcagc cacctccagc accgccccca gcacatctgc cataaacgac
    64861 gtatgcagca gggcatcccc ctgtcttgcc gcccagaatg cctcttccat gtcccgttcc
    64921 ctgtatgatt aattcgtcct gacttcctgc cgcatcctga agcgcagatt ctttttaatt
    64981 tcagcccagc gcgtcatatc tgccggggtc agcggtgtgg ttctggcgta ggtg
//
LOCUS       DAJTXY010000010        60663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTXY010000010 DAJTXY010000000
VERSION     DAJTXY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.010"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..310
                     /locus_tag="OO566_000733"
     CDS             <1..310
                     /locus_tag="OO566_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6604127.1"
                     /translation="ENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSV
                     YKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP
                     "
     gene            423..1217
                     /gene="panB"
                     /locus_tag="OO566_000734"
     CDS             423..1217
                     /gene="panB"
                     /locus_tag="OO566_000734"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6604128.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1229..2080
                     /gene="panC"
                     /locus_tag="OO566_000735"
     CDS             1229..2080
                     /gene="panC"
                     /locus_tag="OO566_000735"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6604129.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2154..3056)
                     /gene="rpnC"
                     /locus_tag="OO566_000736"
     CDS             complement(2154..3056)
                     /gene="rpnC"
                     /locus_tag="OO566_000736"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6604130.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3330..3710
                     /gene="panD"
                     /locus_tag="OO566_000737"
     CDS             3330..3710
                     /gene="panD"
                     /locus_tag="OO566_000737"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6604131.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3714..4943)
                     /locus_tag="OO566_000738"
     CDS             complement(3714..4943)
                     /locus_tag="OO566_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6604132.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5007..5447)
                     /locus_tag="OO566_000739"
     CDS             complement(5007..5447)
                     /locus_tag="OO566_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6604133.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5552..6322)
                     /locus_tag="OO566_000740"
     CDS             complement(5552..6322)
                     /locus_tag="OO566_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6604134.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6319..7245)
                     /locus_tag="OO566_000741"
     CDS             complement(6319..7245)
                     /locus_tag="OO566_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6604135.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7354..8016
                     /gene="can"
                     /locus_tag="OO566_000742"
     CDS             7354..8016
                     /gene="can"
                     /locus_tag="OO566_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6604136.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8057..8593)
                     /gene="hpt"
                     /locus_tag="OO566_000743"
     CDS             complement(8057..8593)
                     /gene="hpt"
                     /locus_tag="OO566_000743"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6604137.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8799..11189
                     /gene="gcd"
                     /locus_tag="OO566_000744"
     CDS             8799..11189
                     /gene="gcd"
                     /locus_tag="OO566_000744"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6604138.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHAFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11236..12785)
                     /gene="cueO"
                     /locus_tag="OO566_000745"
                     /pseudo
     CDS             complement(11236..12785)
                     /gene="cueO"
                     /locus_tag="OO566_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12951..13298
                     /locus_tag="OO566_000746"
     CDS             12951..13298
                     /locus_tag="OO566_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6604139.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13404..14270
                     /gene="speE"
                     /locus_tag="OO566_000747"
     CDS             13404..14270
                     /gene="speE"
                     /locus_tag="OO566_000747"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6604140.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14286..15080
                     /gene="speD"
                     /locus_tag="OO566_000748"
     CDS             14286..15080
                     /gene="speD"
                     /locus_tag="OO566_000748"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6604141.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15118..15480)
                     /locus_tag="OO566_000749"
     CDS             complement(15118..15480)
                     /locus_tag="OO566_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6604142.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15656..18253)
                     /gene="acnB"
                     /locus_tag="OO566_000750"
     CDS             complement(15656..18253)
                     /gene="acnB"
                     /locus_tag="OO566_000750"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6604143.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18608..20461
                     /locus_tag="OO566_000751"
     CDS             18608..20461
                     /locus_tag="OO566_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR6604144.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNSITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQATASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20532..21962)
                     /gene="lpdA"
                     /locus_tag="OO566_000752"
     CDS             complement(20532..21962)
                     /gene="lpdA"
                     /locus_tag="OO566_000752"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6604145.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPKAKKK"
     gene            complement(22287..24179)
                     /gene="aceF"
                     /locus_tag="OO566_000753"
     CDS             complement(22287..24179)
                     /gene="aceF"
                     /locus_tag="OO566_000753"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6604146.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPVAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24194..26857)
                     /gene="aceE"
                     /locus_tag="OO566_000754"
     CDS             complement(24194..26857)
                     /gene="aceE"
                     /locus_tag="OO566_000754"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6604147.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27018..27782)
                     /gene="pdhR"
                     /locus_tag="OO566_000755"
     CDS             complement(27018..27782)
                     /gene="pdhR"
                     /locus_tag="OO566_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6604148.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28323..29696
                     /gene="aroP"
                     /locus_tag="OO566_000756"
     CDS             28323..29696
                     /gene="aroP"
                     /locus_tag="OO566_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6604149.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29739..30593)
                     /gene="ampE"
                     /locus_tag="OO566_000757"
     CDS             complement(29739..30593)
                     /gene="ampE"
                     /locus_tag="OO566_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6604150.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30590..31141)
                     /gene="ampD"
                     /locus_tag="OO566_000758"
     CDS             complement(30590..31141)
                     /gene="ampD"
                     /locus_tag="OO566_000758"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6604151.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31229..32122
                     /gene="nadC"
                     /locus_tag="OO566_000759"
     CDS             31229..32122
                     /gene="nadC"
                     /locus_tag="OO566_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6604152.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNDKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32325..32765
                     /gene="ppdD"
                     /locus_tag="OO566_000760"
     CDS             32325..32765
                     /gene="ppdD"
                     /locus_tag="OO566_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6604153.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32775..34160
                     /gene="gspE"
                     /locus_tag="OO566_000761"
     CDS             32775..34160
                     /gene="gspE"
                     /locus_tag="OO566_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCR6604154.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAVQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34150..35352
                     /gene="hofC"
                     /locus_tag="OO566_000762"
     CDS             34150..35352
                     /gene="hofC"
                     /locus_tag="OO566_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6604155.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35387..36430)
                     /gene="guaC"
                     /locus_tag="OO566_000763"
     CDS             complement(35387..36430)
                     /gene="guaC"
                     /locus_tag="OO566_000763"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6604156.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36592..36630)
                     /locus_tag="OO566_000764"
     CDS             complement(36592..36630)
                     /locus_tag="OO566_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604157.1"
                     /translation="MIIKTLKMSARK"
     gene            36655..37275
                     /gene="coaE"
                     /locus_tag="OO566_000765"
     CDS             36655..37275
                     /gene="coaE"
                     /locus_tag="OO566_000765"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6604158.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLIENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37275..38018
                     /gene="zapD"
                     /locus_tag="OO566_000766"
     CDS             37275..38018
                     /gene="zapD"
                     /locus_tag="OO566_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6604159.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38028..38225
                     /gene="yacG"
                     /locus_tag="OO566_000767"
     CDS             38028..38225
                     /gene="yacG"
                     /locus_tag="OO566_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6604160.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38325..38714)
                     /gene="mutT"
                     /locus_tag="OO566_000768"
     CDS             complement(38325..38714)
                     /gene="mutT"
                     /locus_tag="OO566_000768"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6604161.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38774..41479)
                     /gene="secA"
                     /locus_tag="OO566_000769"
     CDS             complement(38774..41479)
                     /gene="secA"
                     /locus_tag="OO566_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6604162.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKLGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41541..42053)
                     /gene="secM"
                     /locus_tag="OO566_000770"
     CDS             complement(41541..42053)
                     /gene="secM"
                     /locus_tag="OO566_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6604163.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42284..43201)
                     /gene="lpxC"
                     /locus_tag="OO566_000771"
     CDS             complement(42284..43201)
                     /gene="lpxC"
                     /locus_tag="OO566_000771"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6604164.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43302..44453)
                     /gene="ftsZ"
                     /locus_tag="OO566_000772"
     CDS             complement(43302..44453)
                     /gene="ftsZ"
                     /locus_tag="OO566_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6604165.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44514..45776)
                     /gene="ftsA"
                     /locus_tag="OO566_000773"
     CDS             complement(44514..45776)
                     /gene="ftsA"
                     /locus_tag="OO566_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6604166.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45773..46603)
                     /gene="ftsQ"
                     /locus_tag="OO566_000774"
     CDS             complement(45773..46603)
                     /gene="ftsQ"
                     /locus_tag="OO566_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6604167.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46605..47525)
                     /gene="ddlB"
                     /locus_tag="OO566_000775"
     CDS             complement(46605..47525)
                     /gene="ddlB"
                     /locus_tag="OO566_000775"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6604168.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47518..48993)
                     /gene="murC"
                     /locus_tag="OO566_000776"
     CDS             complement(47518..48993)
                     /gene="murC"
                     /locus_tag="OO566_000776"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6604169.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49047..50114)
                     /gene="murG"
                     /locus_tag="OO566_000777"
     CDS             complement(49047..50114)
                     /gene="murG"
                     /locus_tag="OO566_000777"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6604170.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNGWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50111..51355)
                     /gene="ftsW"
                     /locus_tag="OO566_000778"
     CDS             complement(50111..51355)
                     /gene="ftsW"
                     /locus_tag="OO566_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6604171.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51355..52671)
                     /gene="murD"
                     /locus_tag="OO566_000779"
     CDS             complement(51355..52671)
                     /gene="murD"
                     /locus_tag="OO566_000779"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6604172.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52674..53756)
                     /gene="mraY"
                     /locus_tag="OO566_000780"
     CDS             complement(52674..53756)
                     /gene="mraY"
                     /locus_tag="OO566_000780"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6604173.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53750..55108)
                     /gene="murF"
                     /locus_tag="OO566_000781"
     CDS             complement(53750..55108)
                     /gene="murF"
                     /locus_tag="OO566_000781"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6604174.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIARTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEDCHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55105..56592)
                     /gene="murE"
                     /locus_tag="OO566_000782"
     CDS             complement(55105..56592)
                     /gene="murE"
                     /locus_tag="OO566_000782"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6604175.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56579..58345)
                     /gene="ftsI"
                     /locus_tag="OO566_000783"
     CDS             complement(56579..58345)
                     /gene="ftsI"
                     /locus_tag="OO566_000783"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6604176.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58361..58726)
                     /gene="ftsL"
                     /locus_tag="OO566_000784"
     CDS             complement(58361..58726)
                     /gene="ftsL"
                     /locus_tag="OO566_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6604177.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58723..59664)
                     /gene="rsmH"
                     /locus_tag="OO566_000785"
     CDS             complement(58723..59664)
                     /gene="rsmH"
                     /locus_tag="OO566_000785"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6604178.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59666..60124)
                     /gene="mraZ"
                     /locus_tag="OO566_000786"
     CDS             complement(59666..60124)
                     /gene="mraZ"
                     /locus_tag="OO566_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6604179.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14532 a        16799 c        14913 g        14419 t
ORIGIN      
        1 tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa aaggagtcgg
       61 tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga aacctaatga
      121 ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa caggaggagt
      181 ttacccggat caagatacag gtataacata ccctctccat ttccaggcca cgctacaaca
      241 ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt tccaggtaac
      301 ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt gataaatcat
      361 tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta tcaggatacg
      421 ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa aagcgtttcg
      481 cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa gggcttaacg
      541 tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc actctgccag
      601 taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca ccaaactgcc
      661 tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc tttgaaaacg
      721 ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt gagtggctgg
      781 tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac ttaggtttaa
      841 ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc aatgaagcga
      901 gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag ctgctggtgc
      961 tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg atcccggtta
     1021 ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac gacgcctttg
     1081 gtattaccgg cggtcacatt cctaaatttg ctaaaaattt cctcgccgaa acgggcgaca
     1141 tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat ccgggcgaag
     1201 aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc cgctgctgcg
     1261 tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc ctactatggg
     1321 taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg ccgatgtggt
     1381 cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc tggcacgtta
     1441 tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg atttagtttt
     1501 cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt acgttgacgt
     1561 tcctggcctt tcaaccatgc tggaaggtgc cagccgtccg ggacattttc gcggcgtttc
     1621 gactattgtc agcacgctgt tcaacctggt ccagccggac atcgcctgct tcggtgaaaa
     1681 agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct tcgatattga
     1741 gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt cccgtaacgg
     1801 ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt taagttcgat
     1861 tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca ttgcggggca
     1921 agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg ccgacacatt
     1981 gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct ggcttggcga
     2041 tgctcgcctg atcgacaaca aaatggtcga gctggcgtaa tacttaactg gcgctacggc
     2101 tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt aacttaaata
     2161 aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc aacgtcttcc
     2221 cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc ctgtcttccc
     2281 tgcggaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt catcatagac
     2341 ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg ttcagtatga
     2401 ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt gtacccttcg
     2461 tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg aatatgtttt
     2521 tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc cggtgtgatg
     2581 gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc cggcgagtaa
     2641 aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg ataaaacagt
     2701 atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg catggcagca
     2761 atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg gtgttcaatc
     2821 acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac gtccgtgctg
     2881 tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt gttgagatca
     2941 caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc cgtctccgca
     3001 tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc atccatcttc
     3061 cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc tatcgtcagc
     3121 cacctcttta ttgtttaccg agcagcattc ataaaaatat caattcgtta caggcgttac
     3181 atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga tgagtagaca
     3241 ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac cgcctgcttc
     3301 gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag ggcaaactcc
     3361 accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc attgaccagg
     3421 attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg aatgtcacca
     3481 acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga attatttctg
     3541 ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc gccagcttcg
     3601 ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat tttgaaggcg
     3661 acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga ttgttatccc
     3721 tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct taagatataa
     3781 agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat ggttgtcacc
     3841 gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa gccgccaaac
     3901 ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg tgaacgtgca
     3961 aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg ataaccatct
     4021 acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat ttcgttaagc
     4081 tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg acgtttgatg
     4141 cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac aggatacgca
     4201 tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg cgcagggaga
     4261 ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc gtatcccctg
     4321 tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt cgaagtatgg
     4381 cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac cggcaggcca
     4441 ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat acggatctga
     4501 taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg caaattgtcc
     4561 gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg cgtatctttc
     4621 caggtaatta agttctgatt actcagcggc ttgttaaggt cacccaaacc gtcttcaact
     4681 ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga taaacccttt accaaagcca
     4741 aaattaaatg cgtaataact tgcggcagtg ggctctacgg caatgatttg tccggcccga
     4801 atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc attttcgatg
     4861 gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag catcagcagg
     4921 agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg cttttattgt
     4981 agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt gaggacgaaa
     5041 ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc cagttcgaca
     5101 atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat ctgttcaatt
     5161 aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct taataatgaa
     5221 gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc ctcaccagag
     5281 tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct taatccgcgc
     5341 cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag cgcatccagt
     5401 atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc cttcctcctt
     5461 agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt caaaactcct
     5521 ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt ccacgttgga
     5581 tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg ccaaaggtag
     5641 tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg atcatataaa
     5701 cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc agtgagtaaa
     5761 agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg ctgatgtcat
     5821 cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc accgccgtga
     5881 gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca aaaaacagtg
     5941 aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg ccgacatatc
     6001 cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata ttacgctgga
     6061 acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc accgacatca
     6121 tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg ccaatacgcg
     6181 aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact ggcggcacca
     6241 gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg ctttttagcg
     6301 ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca gtgaaacaaa
     6361 cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc cctgctcact
     6421 taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt ccagcgtcgc
     6481 agtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt tcggtgcgag
     6541 atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg aggtattttc
     6601 caccagctca ccatgttgaa taatgccgat attgagacac agcatttctg cttcttccag
     6661 gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca aaaagcccca
     6721 cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa tcagtagttt
     6781 aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc cagataacat
     6841 acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt tttcgctgcg
     6901 gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca caatttgctg
     6961 cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact gacgtttagc
     7021 gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg aggttttatt
     7081 taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg gcccgagaag
     7141 cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct gaacgccgcc
     7201 tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt tttacttacc
     7261 ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta tattagccaa
     7321 acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac actcatcagc
     7381 aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt tgagaaactg
     7441 gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg cgttcctgca
     7501 gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt tgctaacctg
     7561 gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga tgtactcgaa
     7621 gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc cgccgttgaa
     7681 aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa
     7741 catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg
     7801 aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa
     7861 cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg cttgctgcgt
     7921 gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg tcacgggatt
     7981 tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca
     8041 tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac ggcagatgac
     8101 ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac
     8161 cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa
     8221 tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc
     8281 ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct tcatccagat
     8341 ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca
     8401 taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg
     8461 agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa
     8521 tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta
     8581 cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc acgggcaggt
     8641 tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt cattcgcgga
     8701 atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaatct ataattaatt
     8761 gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacaggct
     8821 cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac
     8881 tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc
     8941 ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg ctttggctat
     9001 acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct
     9061 gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt
     9121 ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtcgcac
     9181 tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag gagatcaacg
     9241 gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc gatcaggact
     9301 ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag caaattcacg
     9361 ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc
     9421 cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc gatacccttt
     9481 acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc aaagagaaat
     9541 ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc tgccgtggcg
     9601 tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc
     9661 gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct gaaaacggca
     9721 aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca
     9781 ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa accatcgtga
     9841 tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt
     9901 ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg
     9961 caatcccgtc tgacgaacac gcctttacct ttaactcacc aaactcgtgg gcaccagcgg
    10021 cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg ccggatatct
    10081 ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg
    10141 ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg tgggacatgg
    10201 atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccgatta
    10261 tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat ggcgaactgg
    10321 tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc
    10381 ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata
    10441 tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct
    10501 atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg ggtaacctgg
    10561 ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc
    10621 caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac ccgatggagc
    10681 agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac
    10741 cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag
    10801 catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta
    10861 ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg ttcaatatgg
    10921 gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta
    10981 cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc
    11041 gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc aagcagtacg
    11101 tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac tatattgtgg
    11161 cttatgccct gccggatgat gtgaaataac cataatgccc ggagaaatcc gggcatattt
    11221 ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag
    11281 cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa acttcaccag
    11341 cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc gatgagccgc
    11401 tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat ggaacggatg
    11461 cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt tcgccgccgc
    11521 aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat ggtggaaatc
    11581 gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc ccatcatctg
    11641 gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca tctgcatccc
    11701 catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag
    11761 cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg cactaatagc
    11821 aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg ccatccccat
    11881 ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac
    11941 ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt caggtagcag
    12001 accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga aattgagcga
    12061 gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg cagcgtgttg
    12121 tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg cggcggtcat
    12181 cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct gaacgatcac
    12241 cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc
    12301 aatcaccacc agaccagcca gccccatcgc cacctgtcgc cggtcttgcc atgttgatgt
    12361 ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca
    12421 cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc cagcccgtgc
    12481 cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac cgctttgccg
    12541 cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcagtt
    12601 ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt acgggcatcg
    12661 gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac tgcacggctc
    12721 cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa atcacgacgt
    12781 tgcatagtta tttccttatt cttaagcagg caaaacaaaa tcaaacgttg agcatagtcc
    12841 ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc
    12901 tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt
    12961 ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt tacgcgctca
    13021 gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt ctgaagaacg
    13081 attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc tttttcgccc
    13141 agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct ctcggtgaag
    13201 acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc aaagccctgg
    13261 cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa
    13321 atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt ttttacgggt
    13381 gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa acgctacacg
    13441 accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag accgatcacc
    13501 aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg gatggcgtag
    13561 tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat gttccgctac
    13621 tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt gccatgctgc
    13681 gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc gatgcgggtg
    13741 tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac gacgatccgc
    13801 gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc cagacctttg
    13861 atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg ttcacctcag
    13921 cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg
    13981 gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc agccattact
    14041 tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt atcatgactt
    14101 ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt attcaggcgc
    14161 gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat acggcagctt
    14221 ttgccttacc tcagtatctg caagacgcac tggcttcaca gccgtcctaa ggagaagata
    14281 agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt
    14341 gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat attgcttata
    14401 tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta
    14461 tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt gccagcgtca
    14521 ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca gaacaccccg
    14581 gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc gtacatacct
    14641 acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt gaagtctcta
    14701 cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg
    14761 atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac ggtatgaagc
    14821 actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat atgaaggcgc
    14881 tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc aagatgttgc
    14941 ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca
    15001 gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc
    15061 gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca gatttattta
    15121 cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct caacgccgca
    15181 tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat cgccagcgaa
    15241 ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg tgtattcatg
    15301 ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt gttccacttc
    15361 atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga ccacttcatg
    15421 ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat
    15481 agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg atgtaattat
    15541 cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa aaatgcccgg
    15601 gaatctcatt caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt acttattaca
    15661 ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc tggttgaagt
    15721 tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag gtctggtact
    15781 cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct gcagaagcca
    15841 ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag gtggaaacca
    15901 ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag cccgggatct
    15961 cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc agttgcgcgg
    16021 cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct ttgtgcgcat
    16081 ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa ccgataaata
    16141 cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc ggatcgttcg
    16201 gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact gccgcgtatt
    16261 ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa
    16321 tacggcgttc caaggtacga cgatcgccgt aaccttccgc gatcatccac ttcagcagga
    16381 cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata gtacaaccag
    16441 cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact ttcagatccg
    16501 gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct ttcttctcaa
    16561 cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga tcgcgcaggg
    16621 tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc atatccagcg
    16681 gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg aaagagatac
    16741 cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc agcagcatac
    16801 ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg ccacggttca
    16861 taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg
    16921 tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca
    16981 gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc attttcggtt
    17041 cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc attttctgcg
    17101 ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac acatcactgt
    17161 gcggcagacc aagtgcttca cgagctttgg tggtcaggcc acgcccgata atcagcggaa
    17221 tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca
    17281 gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaatcacgt
    17341 cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg tcttccatcg
    17401 tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg cgtttgttcg
    17461 gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg gatttacgcg
    17521 atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag
    17581 cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata ccttcacggg
    17641 cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac gcatccggtg
    17701 ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta acggtcagtt
    17761 tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcattacct
    17821 gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag ttatcgaaca
    17881 tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca tcatccagcg
    17941 cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt tcgatagctt
    18001 tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc aggaagcctg
    18061 cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac agatctaaca
    18121 ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg gccatttggt
    18181 ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt
    18241 attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct
    18301 tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat
    18361 agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga
    18421 atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt
    18481 gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac cgcctcttta
    18541 tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat
    18601 ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc
    18661 cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg aagcgcctgt
    18721 agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc ttcctgcaac
    18781 ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca ctgcacaaat
    18841 agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc aggtgctgat
    18901 ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg ataatccact
    18961 taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg ccagcgttaa
    19021 atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc aatgggtgca
    19081 aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt
    19141 gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga aagttattac
    19201 cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca taccatccaa
    19261 taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca tttccattga
    19321 gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg tttacgggaa
    19381 ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag aaccgtttgt
    19441 tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt acgcactatt
    19501 cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg atgatgatta
    19561 tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg gcgacaacat
    19621 caatatcgac gtcaacaatt tcaacagtat caccggtgag catcttactg ataagaatat
    19681 ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc
    19741 aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc tacctgctcc
    19801 aacacgcgac agccagcgtc aggcgacagc aagtcagttt cagcaacgaa cacacgccgc
    19861 ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta atgaagctga
    19921 acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac tgacccagca
    19981 gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc gccaggcagt
    20041 ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcag cgcgccagcg
    20101 tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc agactaaccc
    20161 gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa ctcccgaaca
    20221 gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa
    20281 cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc gggagaaagt
    20341 tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga
    20401 gcaacggtca acagtacgcg aacgtctctc tgaacgtgga gcaagaagac tggaaaggta
    20461 aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg
    20521 caaacgaaaa attacttctt cttcgctttc gggttcgggt tcggcaggtc ggtaatgcta
    20581 ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg
    20641 gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccagcccgat ttcacccagc
    20701 agctcgccgc cgttggtacc aacaatcgca ccaccgatca cgcggtgaga ttctttgtcg
    20761 aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc
    20821 cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga
    20881 cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag
    20941 tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt
    21001 gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc
    21061 agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg
    21121 tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc
    21181 ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca
    21241 acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag
    21301 actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca
    21361 atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc
    21421 attaccagca ggcgttctgg cacttctttc agttccagcg cgtcagtgga gtcccagata
    21481 cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc
    21541 gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc
    21601 ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc
    21661 agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg
    21721 gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact
    21781 tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca
    21841 agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca
    21901 gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc
    21961 atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg
    22021 gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc
    22081 gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc
    22141 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    22201 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    22261 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    22321 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    22381 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    22441 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    22501 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    22561 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    22621 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    22681 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    22741 gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    22801 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    22861 aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc
    22921 cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg
    22981 atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    23041 ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    23101 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    23161 aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca
    23221 aattcagatt tgccttccgc tttcgcagcc ggagctgctg ccggagcttc agcttttgct
    23281 gccggagccg gcgctgccgc ttcctgtttc gcaggagctg ccgcaggtgc tgcgccttca
    23341 acttcgaaga tcataatcag cgaaccagtt ttcactttat cgccaacgtt gactttcagt
    23401 tccttcacga cgcctgcaaa cggagccgga acttccatag aagctttatc gccttctacg
    23461 gtaatcagtg actgttcagc ggcaactttg tcgcccactt tcaccatcac ttcggtcact
    23521 tcaacttcgt caccgccgat atccggaacg ttaacttctt tcacgccagc tgctggtgcg
    23581 ggggtcgctg ccggagtcgc ttcctgttta gccgccggag ctgccgcgcc tgcttcaccc
    23641 gcgacttcga agaccataat cagcgagccg gtagacactt tgtcacccac gttcactttg
    23701 atctctttca cggtacctgc aaacggagcc ggaacttcca tagaagcctt gtcgccttct
    23761 acggtgatca gcgactgttc agcttcaact ttatcgccaa ctttcaccag gatttcggtc
    23821 acttcaactt cgtcgctgcc gatatccgga acgttaacgt cttttgccgc cgcagccgcg
    23881 ggtgctgcta ccggagctgc ttctttcttc tcttctgcct gagcaggtgc agcgtctgct
    23941 gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg tctgggtttt atcgccaaca
    24001 gagactttga tctctttaac gatacccgcc tgcggagatg gaacttccat agaggctttg
    24061 tcgccttcta cggtgatcag cgactgttcg gcttcaactt tgtcgcccac tttgaccagg
    24121 atctcggtga tttcaacttc atcagccccg atgtccggta ctttgatttc gatagccatt
    24181 attcttttac ctcttacgcc agacgcgggt taactttatc tgcatcgatg ttgaatttgg
    24241 cgattgcgtc agcaaccact ttcttatcga tttcgccacg tttagccagt tcgcccagcg
    24301 ccgcaaccac gacataagaa gcatcaactt cgaagtggtg acgcaggttc tcacggctgt
    24361 cggaacgacc gaagccatca gtacccagta cgcgatagtc gtcagccggt acgtaagtac
    24421 ggacctgctc agcgaacagt ttcatatagt cggtagatgc cactgccggc gcgtcgttca
    24481 tcacctgagc gatatacgga acgcgcggag tttccagcgg atgcagcatg ttccagcgtt
    24541 cacaatcctg accatcacgc gccagctcgg tgaaggaggt cacgctataa acgtcagaac
    24601 ctacgccgta atctttcgcc aggatctctg ccgcttcacg gacgtgacgc aggatggaac
    24661 cggagcccag cagctgaact ttacctttgc taccttcaat ggtttcgagt ttgtagatac
    24721 ctttacggat accttcctca gcaccttccg gcattgccgg catgtggtag ttttcgttca
    24781 gcgtagtgat gtagtagtaa acgttctctt gtttttcacc gtacatacgc tccagaccgt
    24841 catgcataat gacagcaact tcgtaagcgt aagccgggtc gtaagagata cagttcggga
    24901 tagtcagcga ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttca ccgttcaggg
    24961 tggtacgacc ggaagtaccg ccgatcagga agccacgcgc ttgctggtct ccagccgccc
    25021 agcacagatc gccgatacgc tggaagccga acatcgagta atagatgtag aatgggatca
    25081 tcggcagatt gttcgtgctg taagaggtcg ccgctgccag ccaggaacac cctgcgccca
    25141 gctcgttgat cccttcctgc agaatctgac ctttctcgtc ttctttatag taagcaacct
    25201 gctcgcggtc ctgcggggtg tactgctgac cgttcgggct gtaaatacca atctgacgga
    25261 acagaccttc cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc agacgatctt
    25321 tgatcgactt gttcttcagc atcacgttca gagcacgaac gaaagcgata gtggtagaga
    25381 gctctttgct ctgctcttcc agcagcgcgc cgaagtcttg caggctcggc agctcaagct
    25441 tctcggtgaa gttcggctga cggcttggca gataaccgtg cagtttctga cgctgagcgt
    25501 gcagataggt atgctcttca gaaccttccg ggaaggtgat gtacggcagt ttttcgatat
    25561 ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg acgcacgccg tccatgttca
    25621 ttttcttaac ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg ccgtaacctt
    25681 taatggtatg agcaaggatt actgtcgctt tgcctttggt ttcctgcgct ttcttgaatg
    25741 cagcgtagat tttcttcgga tcgtgaccac cacggttcag tgcccagatc tgctcgtcag
    25801 tccagtctgc aaccagtgct gcggtttcag gatatttacc gaagaagtgt tcacgaacgt
    25861 acgcaccatc tttcgatttg aaggtctggt agtcgccgtc aacggtttcg ttcatcagct
    25921 ggatcagttt accgctggta tctttacgca gcagttcatc ccaacggcta ccccacatca
    25981 ctttgatcac gttccagcca gcaccttcga agatgccttc cagttcgttg atgatcttgc
    26041 cgttaccggt gaccgggccg tcaagacgct gcaggttaca gttgataacg aaaaccaggt
    26101 tatccagttt ttcacgggta gcgatggtga tcgcaccttt ggattccggt tcgtccattt
    26161 caccgtcgcc gaggaacgcg taaacggtct gtttagaggt atctttcagg ccacggtgtt
    26221 ccagatattt caggaattta gcctggtaaa tagcaccaat cggacccaga cccatagata
    26281 cggtcgggaa ctgccagaat tccggcatca gtttcgggtg cggataggaa gagaggccat
    26341 tgccgtgaac ttcctgacgg aagttatcca gctgctcctg agtcagacga ccttccagga
    26401 aagcacgggc gtaaacgccc ggggagatgt ggccctggaa gtaaaccagg tcgccgccat
    26461 cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac atcataaatg gttgcggaag
    26521 actggaagga cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca cgcagcaccg
    26581 tcatgatggc gttccagcgg atagctgaac gaatacggcg ttccagttcc agattacccg
    26641 gatactccgg ttgttcttca acggggatgg tgttgatgta gttgctgata cctgtgcctg
    26701 cggctacgtt tacaccgcct ttgcgggctt cagcaagcag ttggtcgatc agatactgag
    26761 cacgctcaac accttcttca cggatgaccg attcgatcgc ctggagccag tcgcgagttt
    26821 cgatcggatc cacgtcattt gggaaacgtt ctgacatggg ttattcctta tctatctaat
    26881 aacgttgagt tttctggaac ctgtcccatt gaactctcgc cagaaagctc aataagacag
    26941 gttctacgtt tagttaccgc gcttttatat gcgcttgatt tacaacatct tctggataat
    27001 ttttaccaga aaaatcacta attctttcgt tgctccagac gacgcagaga acgctcacgg
    27061 cggctctctt cacggcttct gtcgagcaaa atttcttcga taaaggccag atggcgatgc
    27121 gatgcttcgc gcgcttcttc cggcttaccg gccataatcg cttcaaatat gcgggtgcgg
    27181 tgactactca ccagcggcag catctcgcga cgcgaataga gcaattcgaa gttctggcgg
    27241 acattctggg ccaacatcgg ctccatacac cttagcagat gaagcagaac cacattgtgg
    27301 gccgcttcgg tgacggcaat ctgatactgg agtacggcgt ttgattccgc gtccagatcg
    27361 ccagactgct gcgccagctc tatggcgtgg tggagttcac ggatgcgttc cttgtcttca
    27421 tcggtactac gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg tcgtgtttcg
    27481 agcaagtcat actgtgattc aggatggtcg gagagcagct ccaccagcgg atcgctgaag
    27541 ctttgccata ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag caacaagccc
    27601 ttcgcttcga gacgttgaat cgcctcacgc aaggagggac gggagacgtc gaactgtttt
    27661 gccagttcgc gttccggtgg gagtttttcg cccgggcgga gagtgccttc gaggatcaaa
    27721 aactccagtt gctgctcaat cacatcggag agttttggtt ggcggatttt gctgtaggcc
    27781 atgagttcct gtcttaagcc acttgccgaa gtcaattggt cttaccaatt tcatgtctgt
    27841 gacgctaaag taacaaagta ttcaccttat gtccatacag gttttgattg aaatcatgaa
    27901 actgtgcaca ttttaacaac ttgacatata taacgtttca aagttgtaac tatgcacaaa
    27961 tgtagacttt acgtaggaaa gggatttttt aacgattatt aaactataaa aatccgaatt
    28021 gaaccgattc acttaccaat tttttgattt ttaagtcaat aaaaacgaat ttaaattcac
    28081 tctgcatatt gagaggggtt gaggctgagc tttacaaacg gtttcttttt aagcaactca
    28141 tcttcaacca tgcataaagc gggtgcattc gctgccgcat accattattc ttgatctgac
    28201 ggaagtcttt ttgtaacaat tcaaacttcc ttgatgtaaa taaatcaata caacaaacgg
    28261 aattgcaaac ttacacacgc atcactgcgt agatcaaaaa aacaatcacc gcacgaggtt
    28321 tcatgatgga aggtcaacag cacggcgagc agctaaagcg cggccttaaa aaccgccata
    28381 ttcagcttat cgcgctgggt ggcgcgatag ggacagggct attcctgggt agcgcctccg
    28441 taatacagtc cgcagggcca gggattatcc tgggttacgc cattgctggt tttatcgcct
    28501 ttctgatcat gcgtcagctg ggtgaaatgg tggtcgaaga acctgtcgca ggctccttta
    28561 gccactttgc ttataaatac tggggcagtt ttgccggttt cgcctctggc tggaactact
    28621 gggtactgta cgttttagtt gccatggcag agctgactgc cgtgggtaaa tacattcagt
    28681 tctggtatcc ggaaatccca acctgggttt ctgccgccgt attctttgtg gtgattaacg
    28741 ccatcaacct gaccaacgtt aaagtgtttg gcgagatgga gttctggttt gccattatca
    28801 aagttattgc ggtagtagcg atgatcatct tcggcggctg gctgctgttc agtggcaacg
    28861 gcggcccgca ggcgagcgtt agcaacctgt gggatcaagg tggtttcctg ccgcacggct
    28921 tcaccggact ggtgatgatg atggcgatta tcatgttctc gttcggtggt ctggaactgg
    28981 tggggatcac cgcagcagaa gctgataacc cggagcaaag tatcccgaaa gcaactaacc
    29041 aggttatcta ccgcatcctg attttctata ttggttcgtt agccgttctg ctctcactga
    29101 tgccgtggac ccgcgttacc gccgatacca gtccgtttgt gctgatcttc cacgagttag
    29161 gcgatacctt tgtggcgaat gcgctgaaca tcgtggtact gactgcggcg ctctccgtgt
    29221 acaacagctg cgtatattgc aacagccgta tgctgtttgg tctggcacaa caggggaacg
    29281 cgccaaaagc gctggcgtct gtcgataaac gcggcgtacc ggttaacacc attctggtgt
    29341 ctgcgctggt tacagcattg tgcgtattga ttaactatct tgctccggaa tccgcatttg
    29401 gcctgttaat ggcactggtg gtatccgcac tggtgatcaa ctgggcgatg atcagtctgg
    29461 cgcatatgaa gttccgtcgc gccaagcagg aacaaggcgt ggtaactcgc ttccctgctc
    29521 tgctttatcc gctgggtaac tggatctgcc tgctgtttat ggcggcggta ctggtgatta
    29581 tgctgatgac cccaggaatg gcgatttcgg tatacctgat cccggtatgg ctgatcgtgt
    29641 taggtatcgg ctatctgttt aaagagaaaa ccgccaaagc cgtaaaagcg cattaatctc
    29701 tctacgccct cacccgtaca gggtgagggc aataatctct acaccaacgc cccgtaaatc
    29761 gtcagtagcg caatcaccac cacgaccacg aacgaggttt tcttcgccat tgaaaccgct
    29821 gccttcggcg tctccacctt atcgacatgc ggttcacgcg ccagagagaa ctgcgccaga
    29881 cgcgttaata cctgatattg cgaagtatgg aaatcaccca gcgaagcaaa ccaggccggt
    29941 aacgctttct caccatgacc gatcaaggca tataccacac ccgcaagacg aaccggcacc
    30001 caatccagta catgaagcac tgcatcaatg ccagactgta aacgatgatg cggcgtctgg
    30061 tatcgcgcca gccagtattg ccatgcacgc aagaacgcat accccatcag cgtaacgggt
    30121 ccccaggttc cccccacaat cagccagaac agcggtgcaa gataaaaacg aaagttaatc
    30181 cacagcaatg cattttgcag ctcacgcaaa tactcacgtt cgtcgcagcc tgccgggacg
    30241 ccgtgaatca tggtgagttc gccagccatc gtggcacggg catggctatc attacgtgaa
    30301 gcagctgtca gataagcatg ataatgaaga cgaactttac ctgcgccaat acacagcaaa
    30361 ccaatcagca gccacaccag cagcgtggga acgttgaaca atactccctg caatgcgcgt
    30421 aacagtaaaa aagtcacacc catcgcaata atggtcatgc ctaacgtgcg cccgagagaa
    30481 aaatgtttca cccgccgaaa gaacgcttca agacgatgat caagctgcca gtgctcgccc
    30541 aacttaaaca ggcgctcgaa aattaacacc agtaaggttg taaatagcgt catgttgtct
    30601 ccttgctgac cagcgcacga aaccgtgccc aatcaaatgc aggaccggga tcggttttcc
    30661 gatccggcgc aatatcacaa tggcccgtca tgttattagc gatatcggga tagcgatcta
    30721 tcagtgcccg cgtaaccgcc gcaagctgtt gatactgcgc atcggtatac gccagagtat
    30781 cggtgccttc aagctcaatc ccaatagaaa aatcattgca gcgttcacgc ccctgatact
    30841 gagagactcc cgcatgccag gcacgtttat cgaaaggaac atactggact atttcaccat
    30901 cacggcgaat caaacagtga gcggagacgc gcaaatgggc gatctcagca aagaaaggat
    30961 gtgcctgcgg atcaatagtt ccagtgaata atgcgtcgat ccacggaccg ccaaactcgc
    31021 ctggcggcag gctaatattg tgcaccacca gcagggtggg tgtttcgtca tccaggcggc
    31081 aatcgtaatg tggtgaggga acgcggcgcg cgccaaccag ccacccctgt tctaacaaca
    31141 tgcaggatct ccttatatgt ggtgctaata cccggttcag agtagcatgt ttctacctta
    31201 tgattcgtta gctatctgga gttttaacat gccgcctcgc cgctacaacc ctgacacccg
    31261 acgtgacgag ctgctggaac gcattaatct cgatatcccc ggcgcggtgg cccaggcgct
    31321 gcgggaagat ttaggcggaa cagtcgatgc caacaatgac attacggcaa aacttttacc
    31381 ggaaaattct cgctctcatg ccacggtgat cacccgcgag aatggcgtat tttgtggcaa
    31441 acgctgggtt gaagaggtgt ttattcaact ggcaggcgac gatgtcacca taatctggca
    31501 tgtggatgac ggcgatgtca tcaacgccaa tcaacccttg ttcgaacttg aaggcccatc
    31561 ccgcgtgctg ttaacgggcg aacgcactgc gcttaatttt gtgcaaaccc tttcaggagt
    31621 tgccagtaag gtacgccact atgtcgaatt actggaaggc accaacacgc agttgttgga
    31681 tacgcgcaaa accttacctg gcctgcgttc agctctgaaa tacgcggtac tttgcggcgg
    31741 cggagcgaat caccgtctgg ggctttctga tgccttcctg atcaaagaaa accatattat
    31801 tgcctccggc tcagtgcgcc aggcggtcga aaaagcgtcc tggctgcatc cggatgcgcc
    31861 agtagaagtc gaagtagaga atctggaaga acttgatgag gccctgaaag ccggagccga
    31921 tatcatcatg ctggataact tcgaaacaga acagatgcgc gaagccgtca aacgcaccaa
    31981 cgacaaggcg ctactggaag tgtctggcaa cgtcactgac aaaacactgc gcgaatttgc
    32041 cgaaacgggc gtggacttta tctccgtcgg tgcgctaact aaacacgtac aagcactcga
    32101 cctttcaatg cgttttcgct aattgctgac agatttatcc gctccatcgc gggcggataa
    32161 tctccccttc cccaactttc ttcgtaacgc ctcgcaaatt tcttgtcttt cgctgcaacg
    32221 aaataaaaaa cctcggaatc tgctttcccg tttcgttttt tgcccctcgc ctgtgctgat
    32281 ggcgtttgcc aaagtagcac caaccaaatc aaggagcgaa acagatggac aagcaacgcg
    32341 gttttacact tatcgaactg atggtggtta ttggcatcat tgccatttta agcgccattg
    32401 gtattcccgc ttatcaaaac tacctgcgca aagccgcact caccgacatg ctacaaacct
    32461 ttgtgcctta ccgtaccgcc gtagagttgt gcgcgctgga acatggtgga ttagatacct
    32521 gcgacggtgg cagcaatggc attccctcgc ctaccaccac ccgctatgtt tcagccatga
    32581 gtgtggcaaa gggcgtggtg tcgctgaccg ggcaagaaag tctcaatggg ctaagcgtcg
    32641 tcatgacgcc aggttgggat aacgcaaacg gcgtcaccgg ctgggcgcgc aactgcaata
    32701 ttcaaagtga cagcgcattg cagcaagcct gcgaagatgt cttccgcttt gatgacgcca
    32761 actaaggagc ggcaatgaat attccacagc tcacggccct gtgcctgcgt tatcagggag
    32821 tcttgctgga tgccagcgaa gaagtggttc atgttgcggt ggtcgatgcc ccctcacatg
    32881 agttgctgga cgcattgcat ttcgctacca ccaaacgtat tgagatcacc tgctggacgc
    32941 gccaacaaat ggaaggtcac gccagtcgca cacaacagac attgcccgta gctgttcagg
    33001 agaagcatca gcccaaagca gagttgctga ctcgaacgtt acaatctgcg ctggaacaac
    33061 gcgcgtctga tattcatatc gaaccagcgg acaatgccta ccgcatccgc ttgcgtatcg
    33121 acggcgtatt gcatccttta ccggatgttt caccggatgc cggagtcgca ttaacagcca
    33181 gattaaaagt gctgggaaac ctggatattg cggaacatcg cctgccgcag gacgggcaat
    33241 tcactgtcga actggcagga aacaccgtct catttcgtat tgcgacctta ccatgtcggg
    33301 gtggtgaaaa ggtggtatta aggttgttac agcaggtgag ccaggcactg gatgttaaca
    33361 cgctgggaat gcagccgtta caactggcgg actttgctca tgccttgcaa caaccacagg
    33421 gactggtgct ggtaactggc cctacaggca gcggcaaaac ggtcacgctt tatagtgccc
    33481 tgcaaacgct gaataccgct gacattaata tttgtagcgt cgaagatccg gttgagatcc
    33541 ccatagccgg actaaaccag acgcaaatcc atccgcgtgc cgggctcacc tttcagggtg
    33601 ttttgcgtgc gttattgcgc caggatcctg acgtcatcat gatcggagag atccgcgatg
    33661 gcgaaacggc agaaattgcc attaaagccg tgcaaaccgg tcacctggtg ttgtccacgc
    33721 tacacaccaa ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gttgcccgct
    33781 ggatgctatc atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc
    33841 cacattgtcg ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc
    33901 cgctgcccca ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta
    33961 ccgccttgtt tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata
    34021 ctgaagttga atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa
    34081 acggctgcct ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg
    34141 ggatgccgca tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat
    34201 gcgcaagatg ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa
    34261 cagatggtta ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga
    34321 gataaaagcg cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt
    34381 tctgaagggc tggctctgct ggcggaacag catcccagta aacaatggca agcgttgctg
    34441 caatcgctgg cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg
    34501 tcagaggcat ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag
    34561 ctggatgaat gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac
    34621 aaagtgaaat cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt
    34681 gtggcaatgc tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc
    34741 ccactaccgg cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc
    34801 tggctgctgg tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccgc
    34861 ccgacctggc ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg
    34921 atgaggggac aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc
    34981 attacttttt tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg
    35041 caacttctga cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta
    35101 aaaaatgccg gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc
    35161 ggctcgctgg acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg
    35221 ctggcggata acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt
    35281 atcggtacgc tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt
    35341 gggatgggat aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga
    35401 agatgcggtt ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca
    35461 ggcgtgaagc cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg
    35521 tattttcaac cgggcctcgc agcggcagct tgacggtttt accttctgct gcgcggtatt
    35581 ccgcaacgcc gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca
    35641 taaatttctc gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca
    35701 tgccgccaag catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg
    35761 tggtacagcc accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt
    35821 cgattaccgc agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag
    35881 aacctgggcc aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca
    35941 tttcaccagt cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt
    36001 tcgcaacgaa ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt
    36061 ttaatgccgg gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc
    36121 cggtagaaac catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc
    36181 actcttcgac agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag
    36241 aggccataga aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc
    36301 agctctgacc tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt
    36361 taagagtgga gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt
    36421 caatacgcat gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt
    36481 gacgctatca tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc
    36541 tacaatccca taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg
    36601 cgcgctcatt tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg
    36661 tatatagttg ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt
    36721 gctgatctcg gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca
    36781 ggtgcacctg cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat
    36841 ggaacattgc agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac
    36901 tggcttaacg acctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa
    36961 gcaacttccc cctatgtact gtgggttgtg ccattgctga tagaaaactc actgtataaa
    37021 aaagcgaatc gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg
    37081 cagcgcgatg atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc
    37141 gaagcccgcc ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc
    37201 gcatcggatg ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca
    37261 caggaaaaac cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc
    37321 gtacatggct gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg
    37381 accacgctgg cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc
    37441 gcggcgaagt ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga
    37501 cctggattgg cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa
    37561 aagcggcggg gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc
    37621 gtttgattgc tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt
    37681 tacctacatt gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa
    37741 cctggattgc cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc
    37801 gccagtcggc ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg
    37861 gcgatgccga cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt
    37921 ccggacataa gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg
    37981 taccggaacg tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta
    38041 ttacggtgaa ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc
    38101 ggccattttg ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa
    38161 aacgaatccc cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc
    38221 agtgacattt gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg
    38281 catcttgtgc aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc
    38341 ttcgcaatta ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac
    38401 atccactcac cgggttgccc ttctttaccc cacggcaccc cttcccaact ttcgaccagc
    38461 cagaaccaca gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa
    38521 aaatgttgtg gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc
    38581 gtttcgccca tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct
    38641 gctgcgcgac gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt
    38701 tgcagctttt tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt
    38761 caactgttag cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc
    38821 acgggcaagg atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg
    38881 cggctgcaga gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt
    38941 ccatacgacg ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc
    39001 tcagcgtact gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg
    39061 attcacgttt gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct
    39121 gacgcagata gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca
    39181 tgacgccttt ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat
    39241 acacttcgat ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt
    39301 ctttatccag ccactcggca attggtaaat cgaggtcgaa atcgttcttc agacgttcct
    39361 gcagccccgg aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg
    39421 tggctttgaa cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca
    39481 gcagttcgtt acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt
    39541 ccagcagttg cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa
    39601 tcgctttagt cacccacggg tgttcaatgg cttcgcctag cttcataccc agtttacgca
    39661 tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt
    39721 agaaacggga agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga
    39781 tacgacggga ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg
    39841 catcgtgacg tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt
    39901 ccagcgcggc aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg
    39961 ccatgttggt cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt
    40021 cgttggcgtg gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt
    40081 ttgacaccag ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg
    40141 cagtacgttc tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga
    40201 ccaggtccgg cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca
    40261 gtttgtagat ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca
    40321 gtttttcata cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt
    40381 tctggatctg cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc
    40441 ggccctgcat ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga
    40501 tgtagtcgac gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca
    40561 tcagcatgat gttggccgga gagtacagag actccccttc atccatgatg ccctctttca
    40621 ccagcagttc ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt
    40681 cgtccaccga gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga
    40741 tcaggtgcgg aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc
    40801 cggaaatgat cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca
    40861 gcgcatagtg cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca
    40921 ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc
    40981 gctttgccgg tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa
    41041 acagcggacg gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt
    41101 gcacgccttt accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac
    41161 cttcaccggt acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact
    41221 gaacgtcgaa gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag
    41281 cttccgggat cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg
    41341 ttttcccttt cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga
    41401 tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag
    41461 ttaacaattt gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat
    41521 gaagtaaagg tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc
    41581 tggcttatcc agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg
    41641 cgataaccct tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt
    41701 aatgcaagat gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt
    41761 gccattgcga aagaaagatg acggattacc gtgcgaatag catgttgatg ccagtaatca
    41821 acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag
    41881 ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg
    41941 gcggcgttgc tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc
    42001 ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg
    42061 ccatcccgat cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc
    42121 cacagcacga atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg
    42181 ttttttatag ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt
    42241 gccagatttg ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg
    42301 aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg
    42361 cttcctgttt cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat
    42421 aagcggtaaa tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga
    42481 gcattttgtg acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat
    42541 aatcgtcaac aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca
    42601 gatattcgat atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg
    42661 catcagcgga gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa
    42721 aatcgatggt gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc
    42781 catcttcgac acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt
    42841 cgataccggc gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga
    42901 tttccggcgc gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat
    42961 tgaggtgctc tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca
    43021 tggtatcacg cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc
    43081 gacgatagat gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc
    43141 cggtatgtaa accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca
    43201 tcgtattatc tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag
    43261 tttagcacaa agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt
    43321 aggaatgctg ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc
    43381 actactttag caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag
    43441 cgatccatca ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg
    43501 tccatgccga tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca
    43561 agagaagtac cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct
    43621 accgtttcga actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg
    43681 cgcgcgccag acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct
    43741 tcttccgcac ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca
    43801 gacattacgg tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt
    43861 tcggcgatac cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc
    43921 agggagatac cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag
    43981 tccacatgct tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct
    44041 tcaaagttga aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact
    44101 tcagcgacga ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag
    44161 accatgtctg caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg
    44221 cggccaactt ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga
    44281 atcgtctgtc caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat
    44341 tcaacacctt caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg
    44401 acgccgatga ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct
    44461 ctccgatttg tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac
    44521 tcttttcgca gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt
    44581 ttttctactt cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc
    44641 gccgtcgaat aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg
    44701 ccgatacgca cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc
    44761 gctgcgccac cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga
    44821 agcttttcct gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc
    44881 ggctcgatca cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg
    44941 ctcggcactt ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga
    45001 actttaatcg cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc
    45061 acgacattgc cagcataagg aattacctta gtgtggcgta atgccccacc ggtataaacg
    45121 gcgatatcca ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct
    45181 tccgtcaata ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc
    45241 ccacaacgtt caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg
    45301 tgcacttttg cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga
    45361 tagtcaatcg catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc
    45421 accgatttcg cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa
    45481 ataggcacca taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat
    45541 accgaagaga tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac
    45601 ttgaccacgg attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg
    45661 cccacgccaa tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc
    45721 ttcgcggtac caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt
    45781 gttctgcctg tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc
    45841 ctactgccgc tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt
    45901 gcgcctgctg ctgtaaaacc ggataaagtt ctacaaagcg agccaaacgt ttcatcgtat
    45961 cgccccggcc aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg
    46021 cggtcatcgc cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc
    46081 gatagccctg caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct
    46141 gcttgctggt gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt
    46201 gatcattcca ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc
    46261 actgctttct gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga
    46321 tgatgttgac atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc
    46381 ggatatcgtc attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca
    46441 ggcgttgcgc atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta
    46501 aaacggtcag caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa
    46561 cctcttcttc gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc
    46621 agaattcgta ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc
    46681 aggctgtggc tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca
    46741 ctgtccagca taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc
    46801 agcactaatg cctgcaaatt ggcctcttgt gacgctttca gacctgcggg gcagaaatac
    46861 tgtgtctcat cagagagata cttcgcctca taatcataga aggttccgga cggttgaata
    46921 cgtattgacg gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc
    46981 catttttcaa tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga
    47041 gcattttctg ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg
    47101 ataaccggca aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca
    47161 aactctgcgc gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga
    47221 agtttgctgc gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa
    47281 ggcaagccca tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt
    47341 agcgcgataa acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc
    47401 gggtcgacag gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca
    47461 gaattcagag aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca
    47521 gtcatgttgt tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc
    47581 aatttttccg atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg
    47641 tgccagcatc tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg
    47701 tccacgaatt gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc
    47761 cggatacact tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc
    47821 atacaggtcg cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc
    47881 cggccagcct gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta
    47941 gtcatcgacc agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc
    48001 gaggaaatca aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc
    48061 gtcgtcaatt ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg
    48121 acggcctggc gcattcaggg tgacgcgcat cggctctttg tcctgacgca gcagcgtaaa
    48181 gtgcccctgc gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa
    48241 gccgtaagtt gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc
    48301 aacacacatc accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg
    48361 ttttaaattc tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac
    48421 aatcgccacc atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat
    48481 caggtaccga ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt
    48541 aacgaaggtt gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt
    48601 cgttttgccg tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag
    48661 catttcggca cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt
    48721 gtcggcagaa atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg
    48781 atggttgaaa taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc
    48841 taaatcggaa ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat
    48901 accggcacca ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat
    48961 ggaacgcagt tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc
    49021 atacgatgca aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc
    49081 actcgctcgg tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag
    49141 gtttctcgcg accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc
    49201 tcgataattt tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg
    49261 tctttatgtt gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca
    49321 gttaaagcac cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca
    49381 tcaataaatt cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg
    49441 gattgttgcg aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca
    49501 gcaacctgcg gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac
    49561 acacgaaccg gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca
    49621 tcggtacgca ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc
    49681 atcactttgg tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt
    49741 tcatgaagta caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca
    49801 tagccgccca taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga
    49861 cgccagccgt tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc
    49921 aaaccagaga tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc
    49981 atacggtcgg cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca
    50041 accgccagtc ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt
    50101 ccttgaccac tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat
    50161 aatcaatacg caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt
    50221 aactgattag cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt
    50281 taaccagcgc ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac
    50341 ggtggtcaat ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata
    50401 ccattaaaag tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa
    50461 taaagtcagt gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt
    50521 gcccccaaag ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc
    50581 caaagggatc ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg
    50641 cgaggatcag caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca
    50701 atttcgctcc cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac
    50761 caaggtctgg ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca
    50821 ggaagccgcg caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat
    50881 agcaaaacag cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga
    50941 tccaacgcga tgccccttta acagcgctac ctactaccag cacgatcatc agcaggatga
    51001 tagatccgag cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg
    51061 taatgatcgc cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga
    51121 acggatcgtt ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc
    51181 caatcgccgc gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt
    51241 cggtatcttt ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca
    51301 agatactgaa tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc
    51361 taactccttc gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc
    51421 aaggctggca caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag
    51481 caagcgcata gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc
    51541 cagctgcgcg ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg
    51601 cgccagtggg ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc
    51661 gtctacgtgc aggccattca gcgccgcttc cgtactgccg acgttggtcg cttttgaatc
    51721 gttaatccag cgcacaccgt tatgctccag caccacttca aagcgatgcg gcagaccggt
    51781 gaaggtggtt aacgctttca ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc
    51841 cagcgccgcc agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt
    51901 cagcactttc tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata
    51961 gtcacccatg ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt
    52021 taaggcatca tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc
    52081 tgcacgatac tgttgtaaac caaacgggta gcgatccata tgatcttcag tcacgttcag
    52141 aatggtcgct gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc
    52201 cagcacgtac agttcacact catcatccag tagcatcaac gcaggcaggc caatattgcc
    52261 acccacacca acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt
    52321 gcttttgccg ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacaaaacag
    52381 ctcgatatcg ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc
    52441 cagtgcaata ccgggactgg cgacaatcag atctgccgcc atcaaccatt catcattcag
    52501 actgcccgtg tggcgttcta cggcttcggg taatttatcc aggctaggcg gtgtcatacg
    52561 cgtatccata acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc
    52621 ggtgaggccc aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt
    52681 accttcagcg ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca
    52741 atgacgcgcg gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg
    52801 aaaatacgtt gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct
    52861 accacgaaca cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata
    52921 atgcctaacg caccacctaa cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga
    52981 taggtgttaa accacaggaa gcccagtcct gccccgacta tcgcggtaca gacaataacc
    53041 agttccccgg cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt catgttgccg
    53101 gtcgcccacg ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga
    53161 ccgtcaagac catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc
    53221 agcagaatgt agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc
    53281 tgcgttgcgg gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc cagcgcaatg
    53341 accgacatcc agaaatattt ccaacgagcg atcaaccctt tggtatcttt acgcaccact
    53401 ttgcgataat catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac
    53461 cagacgtacg gattggacgg gtaagcccac agcagcacgg agatcacaat cgctgtcagg
    53521 atcataatcc cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg
    53581 ttacgcacta cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc
    53641 cacaatgaga tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa
    53701 aagacgttaa agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc
    53761 cattctcctg taaagcgcgt actacctctt ccatggcagc actacgtgaa cccttaacta
    53821 aaatcgtaat tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt
    53881 tatcagcaaa atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca
    53941 tgcttaacac gcggtcaata ccagcagctt ttgccgcctc gcccacctgt acatggcagt
    54001 cttcgctttc agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca
    54061 tttcagccag tacctgaaca gctgcggtca ttgaaccgac attggcgttg taggagtcgt
    54121 cgagcagcaa ctggttttct gccagttgga tggggaacag acggccagga acagctttca
    54181 gatttgccag ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag
    54241 cggctgccag cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc
    54301 taccggttgg ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg atattggtgg
    54361 cggtgaaatc gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta
    54421 cgctctgcca gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg
    54481 gcaggccgct aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt
    54541 ccagatgcgc cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc agactcacag
    54601 tcctggcaat ctcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg
    54661 gcgttaagcg caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat
    54721 aaagcgtgtt gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc
    54781 cggaggaccc cgtcagagca accacgcgcg ccggaacttg ctggcgaacc catgcagcca
    54841 gttcaccaaa cgccagacgc gtatccttga cgattaactg cggcaggtcg atgtccagcg
    54901 gacggctaac cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg
    54961 catcaaaacg ttcacctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat
    55021 cagtggttac agcatcaagg gtgatatctg caccttgcag ttcaccattg agaatatcgg
    55081 taagttggct aagggttacg ctaatcatgc aatccccccc agcagacgcg ccaccgtgac
    55141 gcgatcggag tagtccagac gctgattgcc aacaatctgg taatcctcat ggcctttgcc
    55201 cgcgaccagt accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc
    55261 acggccttcc atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat
    55321 gatggcacgc ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa
    55381 ctcttcggca attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc
    55441 aaagacgcac cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag
    55501 tgcatccggc gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac
    55561 ttccatacgt ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg
    55621 atagcccagc gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc
    55681 catcagatgg ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc
    55741 gctgtcgtga tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat
    55801 atgatcttcc attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac
    55861 ttcatcgtcg gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt
    55921 cgcagcttcg tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt
    55981 aaagaccgac gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga
    56041 aacttccatt gcgcaaaaag tcgcgccctg atccaccagc cccgccagct catgctgaac
    56101 atcaactgcc gaaccggtag tattttctgt cgggatcact ttccccagca gaccgttacc
    56161 aacggtgccc attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagaagctg
    56221 ggtcgtcgtg gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg
    56281 ttcatggtaa aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat
    56341 gaccggtacg ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgcctctgc
    56401 aataatggca gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg
    56461 atgacctact acagctacaa agagatcgcc cgccgcagcc acacggctgt cgagtgtcat
    56521 ctctcgcagt gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt
    56581 acgatctgcc acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg
    56641 tcagcgcatc cggctcgatg ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga
    56701 ccggcgcgga aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa
    56761 ccagcgcgaa gcgcggctga ctcgcaggcg caacgcctgc ggtataagca atatatttat
    56821 tgatgtagcg accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcgatacgat
    56881 agcctttaat cgccgccttc acgccgccgc cgcctggtag cgccacgctt tccatcatat
    56941 gcaccacggt gcgaacaatg gattccggga agacacgttc accgggaacc ggggggtcaa
    57001 ctttggtaat cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg
    57061 ctaactgtaa cggtgttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt
    57121 cagaccaccg ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg
    57181 tcgcttttcc cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgcta
    57241 acgccagctt ggaaacaccg acgttactcg acttctgtaa taccccggtc agggttaatt
    57301 cgctgtagcg tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga
    57361 gtaccgagtt ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa
    57421 ccgttgagcc cggttcaaac acgtcggtga tggtacggtt acgcatcgcc tctttcggcg
    57481 tgccgctcag attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcacctg
    57541 tgttgacatc caccagcacg gcgctaccag attcagcctt gttaaaggcc accgcattgt
    57601 tcagttcgcg ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg
    57661 ctgcctggct gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca
    57721 caatgcgctc acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa
    57781 tcccttgact atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggacggat
    57841 agtaacggcg agactcttca cgcagatgaa tccccggcag tttcagtttt ttgatgtagt
    57901 ccgccatgtc agagtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt
    57961 taatgcgggc agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc
    58021 ggtcaccgac gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt
    58081 ttaccggcac gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg
    58141 tggaaacttg ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg
    58201 agataacttg taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc
    58261 cgcataacaa cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt
    58321 tctgcgtttt cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat
    58381 tttcttgtga cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc
    58441 ggctatggtc gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag
    58501 catctcgctc cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg
    58561 tggttaccac ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc
    58621 caaatcgcaa aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca
    58681 tcgatccttt aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc
    58741 tgcaatacgc agaactgaac tacgggcacg agggttctca gccacctctt cttcgcccgg
    58801 cattaacttg cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt
    58861 catcggtaac cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac
    58921 aatacggtct tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac
    58981 gttgagcgag ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg
    59041 caccgcctgg aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc
    59101 agccacgact tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac
    59161 aatggcgcgg gcaatgcgtt tggcaaaacg ctcttcacca taggttttca atacccaggc
    59221 gatatcggct tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc
    59281 catacgcatg tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag
    59341 ttgcggtgaa gagacgccaa gatcgaggag aatgccgtcg atcttgccaa taagatcgcg
    59401 ctcggcaacg tattcgccca gcgcggagaa aggtccgtgg atgatggaga agcgcggatc
    59461 atcaatagtc ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc
    59521 ctcttcgcca agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc
    59581 aatgtagatg ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt
    59641 agtatgttta tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt
    59701 ctccggtagc caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt
    59761 catcccacag ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg
    59821 catgttgccg cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc
    59881 tggcatgacc taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcgacagac
    59941 gcgataattt ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt
    60001 gatgaatgtc aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat
    60061 aacgggtagg cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga
    60121 acatgccagc ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt
    60181 acggagcgga ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga
    60241 tgtttacggc taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt
    60301 tatgaatagc tgtaagaaaa aatccctcat cgtcatcact gttttaaaat gactcactat
    60361 tttccacagc aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt
    60421 ccccggttaa cagtccttgt tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa
    60481 agcaaagaaa agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa
    60541 aaacgcgctg tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaacggg
    60601 cgggtttaaa accatttatc aataatagtc aggaatagtc ttatttactt taaggatatt
    60661 gat
//
LOCUS       DAJTXY010000011        58007 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTXY010000011 DAJTXY010000000
VERSION     DAJTXY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.011"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(26..280)
                     /locus_tag="OO566_000787"
     CDS             complement(26..280)
                     /locus_tag="OO566_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6604180.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            435..1007
                     /gene="yeiP"
                     /locus_tag="OO566_000788"
     CDS             435..1007
                     /gene="yeiP"
                     /locus_tag="OO566_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6604181.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1230..2696
                     /locus_tag="OO566_000789"
     CDS             1230..2696
                     /locus_tag="OO566_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6604182.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNPLKI"
     gene            2814..3800
                     /gene="yeiR"
                     /locus_tag="OO566_000790"
     CDS             2814..3800
                     /gene="yeiR"
                     /locus_tag="OO566_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6604183.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3839..4552
                     /gene="lpxT"
                     /locus_tag="OO566_000791"
     CDS             3839..4552
                     /gene="lpxT"
                     /locus_tag="OO566_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6604184.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4964..5530
                     /gene="mepS"
                     /locus_tag="OO566_000792"
     CDS             4964..5530
                     /gene="mepS"
                     /locus_tag="OO566_000792"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6604185.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5711..7267
                     /locus_tag="OO566_000793"
     CDS             5711..7267
                     /locus_tag="OO566_000793"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6604186.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7349..9163
                     /locus_tag="OO566_000794"
     CDS             7349..9163
                     /locus_tag="OO566_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6604187.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9164..10258
                     /locus_tag="OO566_000795"
     CDS             9164..10258
                     /locus_tag="OO566_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6604188.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10258..11283
                     /locus_tag="OO566_000796"
     CDS             10258..11283
                     /locus_tag="OO566_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6604189.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11285..12874
                     /gene="yejF"
                     /locus_tag="OO566_000797"
     CDS             11285..12874
                     /gene="yejF"
                     /locus_tag="OO566_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6604190.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     LSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12878..13222)
                     /locus_tag="OO566_000798"
     CDS             complement(12878..13222)
                     /locus_tag="OO566_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604191.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO566_000799"
     CDS             complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO566_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6604192.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO566_000800"
     CDS             complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO566_000800"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6604193.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15618..17378
                     /locus_tag="OO566_000801"
     CDS             15618..17378
                     /locus_tag="OO566_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6604194.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17503..17787
                     /gene="rplY"
                     /locus_tag="OO566_000802"
     CDS             17503..17787
                     /gene="rplY"
                     /locus_tag="OO566_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6604195.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO566_000803"
     CDS             complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO566_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6604196.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19115..19342
                     /locus_tag="OO566_000804"
     CDS             19115..19342
                     /locus_tag="OO566_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6604197.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19362..21122
                     /gene="pbgA"
                     /locus_tag="OO566_000805"
     CDS             19362..21122
                     /gene="pbgA"
                     /locus_tag="OO566_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6604198.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21197..21273
                     /locus_tag="OO566_000806"
     tRNA            21197..21273
                     /locus_tag="OO566_000806"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21231..21233,aa:Pro,seq:ggg)
     gene            complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO566_000807"
     CDS             complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO566_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6604199.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24287..24934
                     /gene="narP"
                     /locus_tag="OO566_000808"
     CDS             24287..24934
                     /gene="narP"
                     /locus_tag="OO566_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6604200.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO566_000809"
     CDS             complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO566_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6604201.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO566_000810"
     CDS             complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO566_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6604202.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO566_000811"
     CDS             complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO566_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6604203.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO566_000812"
     CDS             complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO566_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6604204.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO566_000813"
     CDS             complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO566_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6604205.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO566_000814"
     CDS             complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO566_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6604206.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO566_000815"
     CDS             complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO566_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6604207.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO566_000816"
     CDS             complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO566_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6604208.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO566_000817"
     CDS             complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO566_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6604209.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO566_000818"
     CDS             complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO566_000818"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6604210.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO566_000819"
     CDS             complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO566_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6604211.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO566_000820"
     CDS             complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO566_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6604212.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO566_000821"
     CDS             complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO566_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6604213.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTAMKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO566_000822"
     CDS             complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO566_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6604214.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO566_000823"
     CDS             complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO566_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6604215.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRVWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37692..38180
                     /gene="eco"
                     /locus_tag="OO566_000824"
     CDS             37692..38180
                     /gene="eco"
                     /locus_tag="OO566_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6604216.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38330..39361)
                     /locus_tag="OO566_000825"
     CDS             complement(38330..39361)
                     /locus_tag="OO566_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6604217.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQSTLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39595..39954)
                     /locus_tag="OO566_000826"
                     /pseudo
     CDS             complement(39595..39954)
                     /locus_tag="OO566_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40172..41815)
                     /gene="yojI"
                     /locus_tag="OO566_000827"
     CDS             complement(40172..41815)
                     /gene="yojI"
                     /locus_tag="OO566_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6604218.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41891..42485)
                     /gene="alkB"
                     /locus_tag="OO566_000828"
                     /pseudo
     CDS             complement(41891..42485)
                     /gene="alkB"
                     /locus_tag="OO566_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42485..43549)
                     /gene="ada"
                     /locus_tag="OO566_000829"
     CDS             complement(42485..43549)
                     /gene="ada"
                     /locus_tag="OO566_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6604219.1"
                     /translation="MKNATCLTDDQRWQSVLARDPDADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43623..44678)
                     /gene="apbE"
                     /locus_tag="OO566_000830"
     CDS             complement(43623..44678)
                     /gene="apbE"
                     /locus_tag="OO566_000830"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6604220.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44790..45911)
                     /gene="ompC"
                     /locus_tag="OO566_000831"
     CDS             complement(44790..45911)
                     /gene="ompC"
                     /locus_tag="OO566_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6604221.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46649..49321
                     /gene="rcsD"
                     /locus_tag="OO566_000832"
     CDS             46649..49321
                     /gene="rcsD"
                     /locus_tag="OO566_000832"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6604222.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQILIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            49338..49988
                     /gene="rcsB"
                     /locus_tag="OO566_000833"
     CDS             49338..49988
                     /gene="rcsB"
                     /locus_tag="OO566_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6604223.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50188..53037)
                     /gene="rcsC"
                     /locus_tag="OO566_000834"
     CDS             complement(50188..53037)
                     /gene="rcsC"
                     /locus_tag="OO566_000834"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6604224.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKKTLTVYAERVRKSRDS"
     gene            complement(53312..54088)
                     /locus_tag="OO566_000835"
     CDS             complement(53312..54088)
                     /locus_tag="OO566_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6604225.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDMDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54093..55742)
                     /locus_tag="OO566_000836"
     CDS             complement(54093..55742)
                     /locus_tag="OO566_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6604226.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55743..58007)
                     /locus_tag="OO566_000837"
                     /pseudo
     CDS             complement(55743..58007)
                     /locus_tag="OO566_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        14166 a        15661 c        14265 g        13915 t
ORIGIN      
        1 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
       61 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
      121 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
      181 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
      241 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
      301 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
      361 ccacttgccc gaaaggccca tcgcgagtac tttgtcgcga tatttttgac attttcgact
      421 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
      481 acggcaaact gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg
      541 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
      601 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
      661 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
      721 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
      781 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
      841 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
      901 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
      961 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
     1021 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
     1081 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
     1141 atcgcattaa caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt
     1201 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
     1261 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
     1321 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
     1381 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
     1441 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
     1501 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
     1561 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
     1621 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
     1681 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
     1741 gccgccgtga gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
     1801 atggccatgt ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg
     1861 ccgggtggat aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
     1921 ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg
     1981 cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc
     2041 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
     2101 aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
     2161 tcgcctatat cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa
     2221 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
     2281 ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga
     2341 tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc
     2401 gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg
     2461 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
     2521 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
     2581 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
     2641 aacaaatcgt acaattcggc gcacatcagg cgctattaaa ccccctcaaa atttaacgat
     2701 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct
     2761 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
     2821 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
     2881 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
     2941 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
     3001 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
     3061 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagctcctcg
     3121 atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc
     3181 tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
     3241 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
     3301 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
     3361 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
     3421 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
     3481 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
     3541 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
     3601 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
     3661 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
     3721 agctgatttc cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc
     3781 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
     3841 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc
     3901 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
     3961 ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgactaacaa
     4021 tcgcgccttc gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct
     4081 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
     4141 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
     4201 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
     4261 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
     4321 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc
     4381 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
     4441 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
     4501 cttttttgac aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt
     4561 aattaacatc atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
     4621 ttcatctatc gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
     4681 tcaattgtac tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata
     4741 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt
     4801 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
     4861 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcctgg cattttttta
     4921 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
     4981 gattttgaga tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc
     5041 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcaa tgggtagtga
     5101 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
     5161 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
     5221 cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt
     5281 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
     5341 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
     5401 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
     5461 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
     5521 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
     5581 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
     5641 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
     5701 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
     5761 tgtagcaggc gtgatgattg cgatactggt tagctgcctt cagtttttag tggccaggca
     5821 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
     5881 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
     5941 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
     6001 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
     6061 caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg
     6121 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
     6181 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
     6241 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
     6301 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat
     6361 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
     6421 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct
     6481 gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt
     6541 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt
     6601 actgctacgc tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt
     6661 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
     6721 cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc
     6781 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
     6841 gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
     6901 agaagccaca caactcttcg cctggctgca cttggtcggc gtagaaattg ctattgatga
     6961 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
     7021 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
     7081 cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac
     7141 gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
     7201 tagtcgcccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca
     7261 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
     7321 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
     7381 tctgttcacc ttcggtgcgc aggcgcaggc tatcaaggaa agctatacct ttgccgtgct
     7441 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
     7501 aaaaggtggg cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc
     7561 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc
     7621 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga
     7681 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat
     7741 tactgcccgc gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt
     7801 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
     7861 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
     7921 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
     7981 tccgtaccac gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
     8041 ttactgggca gcaaacttac cggtaaaccg tggacgctgg catttcgaca ccattcgcta
     8101 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
     8161 gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa
     8221 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
     8281 gtttaatatc caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc
     8341 ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag
     8401 ttattttcaa aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
     8461 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
     8521 ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
     8581 cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct
     8641 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
     8701 cagcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac
     8761 taaccgcatg cgcaatcgcg actatgacat gatgccgcgc ctatggcggg cgatgccgtg
     8821 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
     8881 ccccggcgtg caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg
     8941 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
     9001 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
     9061 ccaacccgct gtacgccctg tttacagcct aggtatcgat acctggtggt atgacgttaa
     9121 taaagcggcc aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat
     9181 tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat
     9241 cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa
     9301 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
     9361 tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat
     9421 cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
     9481 gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg cgtcggtgct
     9541 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
     9601 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt
     9661 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
     9721 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
     9781 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
     9841 gcatatcacg ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct
     9901 gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
     9961 aggggtaagt gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt
    10021 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    10081 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    10141 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    10201 tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg
    10261 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc
    10321 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    10381 gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    10441 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    10501 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    10561 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    10621 tggctgggaa cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg
    10681 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    10741 ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa
    10801 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    10861 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    10921 gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt
    10981 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    11041 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc
    11101 ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    11161 aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    11221 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    11281 tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca
    11341 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    11401 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    11461 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    11521 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    11581 ggaaccgatg ctgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    11641 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    11701 tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    11761 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    11821 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    11881 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    11941 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    12001 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    12061 tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga
    12121 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    12181 tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg
    12241 cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca
    12301 gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc
    12361 tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    12421 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    12481 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    12541 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    12601 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    12661 ggtacaggcg caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta
    12721 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    12781 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    12841 ggagtatacg cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    12901 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    12961 ggcgatttac aaacaggcac ggctcacctt cacactccaa caccgaacat ggaacttcga
    13021 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    13081 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt
    13141 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    13201 ttgagagttg taatgaagtc acttattttc cccggaagta cattcgcttc acaatttgtt
    13261 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    13321 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    13381 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    13441 actttttaca taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg
    13501 tggttgacat gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg
    13561 ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    13621 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    13681 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    13741 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    13801 caccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat
    13861 ccacgctgcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    13921 gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc
    13981 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc
    14041 ggcgcttaag aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag
    14101 gacacgttta tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg
    14161 ctgacgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag
    14221 aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    14281 gccgccaact atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat
    14341 catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct
    14401 ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg
    14461 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg
    14521 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    14581 aatataagta ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat
    14641 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    14701 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    14761 gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    14821 gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac
    14881 gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    14941 cagacgaacc tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc
    15001 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc
    15061 cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    15121 ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc
    15181 gtgcagtttc cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc
    15241 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg
    15301 ctgcgccagc gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc
    15361 attacgaacg atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    15421 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    15481 gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta
    15541 tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa
    15601 tctacctacg cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg
    15661 ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg
    15721 caggtaaaag cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc
    15781 tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg
    15841 aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg
    15901 gcagcgttca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga
    15961 ttgtcgatga atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca
    16021 ctcacctgac taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc
    16081 gattgggcaa aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga
    16141 aagccctttt ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct
    16201 atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc
    16261 aggcacagag taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc
    16321 aacgcattac gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg
    16381 taatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg
    16441 aagatgctgc actgattact ggcgaccccc ccggcgctga gcgcgatgtg ttaattgaag
    16501 attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg
    16561 acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc
    16621 aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg
    16681 attatgcggg taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa
    16741 gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg
    16801 ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt
    16861 ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc
    16921 cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac
    16981 tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac
    17041 gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca
    17101 cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc
    17161 agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc
    17221 tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt
    17281 ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg
    17341 aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata
    17401 aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt
    17461 tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca
    17521 gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc
    17581 ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa
    17641 gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctgaccat cgttgttgac
    17701 ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg
    17761 cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg
    17821 cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca
    17881 gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga
    17941 cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc
    18001 ggttgctgga tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa
    18061 gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa
    18121 gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac
    18181 gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc
    18241 attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc
    18301 ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa
    18361 tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc
    18421 tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat
    18481 acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt
    18541 ttcgttaacg cgcatactgc tcaggttgct cagaactgcc accagcagat actccaccgc
    18601 cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa
    18661 ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg
    18721 acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt
    18781 atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg
    18841 ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg
    18901 gtgcagagca atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg
    18961 tattcaacca ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca
    19021 gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata
    19081 tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa
    19141 tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc
    19201 gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat
    19261 tgccccggcc caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat
    19321 caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc
    19381 gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata
    19441 ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc
    19501 ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc ctggtgttcg
    19561 ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga
    19621 ggtttttgtc cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag
    19681 tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag
    19741 acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat
    19801 tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc
    19861 gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg
    19921 tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc
    19981 cgctttcgta cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc
    20041 aggagtatca acgccgtctt attgagcaag gtaatccaga caccgtttcc gtacagtatc
    20101 cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg
    20161 tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg
    20221 agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct
    20281 ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc
    20341 ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag
    20401 atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg
    20461 tacgcaccca atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg
    20521 cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca
    20581 gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga
    20641 tcaaacgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta
    20701 tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg
    20761 gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg
    20821 ctctgactga tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca
    20881 cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact
    20941 gggttactgc tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc
    21001 tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa
    21061 aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact
    21121 gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt
    21181 attagcagcc acgagtcggc acgtagcgca gcttggtagc gcaccgtcat ggggtgtcgg
    21241 gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta
    21301 cggttggttt ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct
    21361 gtttattttc tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc
    21421 tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga
    21481 tgaccttatc ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc
    21541 atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag
    21601 ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc
    21661 ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta
    21721 gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc
    21781 gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc
    21841 aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt
    21901 caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt
    21961 atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc
    22021 aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag
    22081 cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg
    22141 ggtgttaatt gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag
    22201 acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt
    22261 cagcacatcg gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg
    22321 gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt
    22381 gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc
    22441 tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt
    22501 gacgttgagc tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt
    22561 tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga
    22621 acgagtcact ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa
    22681 gttgaggcga gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt
    22741 ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc
    22801 ctcagaaacc actaccttac ccaccaacat agcgggtgca taatttattt gcggcttcat
    22861 gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac
    22921 attagcgcgg ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat
    22981 acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc
    23041 agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtgt caatagcctg
    23101 cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc
    23161 atcctttata ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga
    23221 gacaattaat ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac
    23281 ctccagtaag ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg
    23341 caaacttccg ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg
    23401 accgagacgg tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc
    23461 tgctttctga tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt
    23521 ttcgatagcg gcaaaaacgg tccccccatc tttttcaacg attaagcgcc cgcctttaat
    23581 cgtggtatca ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt
    23641 tgccgcgcca cccatttgaa caatttgata accatcattt aatactgagt attctgcggt
    23701 accattcatt tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa
    23761 ttcttttata tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac
    23821 cagctggata tctttttcca ggataacacc atcaactgtc tcaccatact caactgttga
    23881 tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    23941 aagagaaaca gaaccagatt gatgcattga gctttcatcc tatgaaatta attgcagtta
    24001 aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg
    24061 tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct
    24121 gatgcaaaat tccgtcttta taatgaaaat gatgccaaag cgaacgacaa ggttgtagtt
    24181 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac
    24241 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    24301 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    24361 actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgacggcg cgagcgcgat
    24421 cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat
    24481 gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    24541 cctgaccgta tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg
    24601 ttatctgttg aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa
    24661 aggcaacaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg
    24721 cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct
    24781 ggcacagggg ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt
    24841 aaaagtacat attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac
    24901 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg
    24961 gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg
    25021 atcgatcaat ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat
    25081 ccggcataaa ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct
    25141 ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa
    25201 tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa
    25261 cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga
    25321 tattgctatg gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt
    25381 tgggatcaga tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg
    25441 gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca
    25501 tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag
    25561 tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt
    25621 ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct
    25681 gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac
    25741 ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg
    25801 cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc
    25861 caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt
    25921 aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac
    25981 cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat
    26041 cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact
    26101 gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg
    26161 agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt
    26221 ctcccacagc ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg
    26281 atagcgaatg ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag
    26341 atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc
    26401 tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat
    26461 gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt
    26521 cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg
    26581 ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga
    26641 ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc
    26701 ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc
    26761 ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt
    26821 ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa
    26881 agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc
    26941 gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt
    27001 ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca
    27061 ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca
    27121 tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg
    27181 ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa
    27241 taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc
    27301 accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg
    27361 aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa
    27421 ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt
    27481 agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa
    27541 atactgccca gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc
    27601 agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc
    27661 gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg
    27721 ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac
    27781 gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag
    27841 aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc
    27901 gccagtgagt gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc
    27961 ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc
    28021 acaatgccga gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca
    28081 taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag
    28141 aaacccacgt accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac
    28201 agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga gaacgggtta
    28261 gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca
    28321 cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg
    28381 ctcatcagca gcacccacag cagaagcgaa ccttcatgcg cgccccaggt agccgccacg
    28441 cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg
    28501 ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc
    28561 caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc
    28621 ggatacacgg acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt
    28681 tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca
    28741 tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg
    28801 cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga
    28861 acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg
    28921 tgaaggtcac tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc
    28981 caacgcgcag acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa
    29041 tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga
    29101 tagtcagcgc cagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa
    29161 tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg
    29221 cgccacacca cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag
    29281 cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa
    29341 aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca
    29401 ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc
    29461 agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc
    29521 ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg
    29581 gagtaatgaa taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgg
    29641 cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac
    29701 agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt
    29761 tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc
    29821 atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc
    29881 accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata
    29941 atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag
    30001 ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt
    30061 tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca
    30121 gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg
    30181 tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc
    30241 catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg
    30301 ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg
    30361 tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca
    30421 gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa
    30481 ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg
    30541 cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac
    30601 ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg
    30661 gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta
    30721 caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca
    30781 gttcaagacg gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg
    30841 gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc
    30901 ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc
    30961 ggtaaaaggc tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc
    31021 gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag
    31081 cccggcctgc gccaaggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg
    31141 cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag
    31201 gttttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc
    31261 agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt
    31321 gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa
    31381 ggttcgttca tcccgctcac aaagtacctc tctggcttca agcataccca cgcaataacc
    31441 ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg
    31501 gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga
    31561 tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt
    31621 attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt
    31681 ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt
    31741 gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac
    31801 gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat
    31861 ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc
    31921 cccccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg
    31981 gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt
    32041 tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat
    32101 tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac
    32161 ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc
    32221 agtggtgcga tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac
    32281 ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat
    32341 ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga
    32401 ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat
    32461 cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa
    32521 ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac
    32581 gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt
    32641 agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga
    32701 tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc
    32761 ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg
    32821 agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg
    32881 gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc
    32941 aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg
    33001 cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat
    33061 cgctttcccg ccaaggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg
    33121 gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg
    33181 gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga
    33241 ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg
    33301 tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt
    33361 gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag
    33421 tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc
    33481 gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg
    33541 cgtgttgcgc tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca
    33601 aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag
    33661 ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac
    33721 tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg
    33781 cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc
    33841 ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc
    33901 gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac
    33961 acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc
    34021 attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca
    34081 cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac
    34141 cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc
    34201 gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc
    34261 acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa
    34321 ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac
    34381 gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa
    34441 cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc
    34501 cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac
    34561 caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc
    34621 gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag
    34681 ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac
    34741 ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc
    34801 cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg
    34861 ccagagatcc gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg
    34921 gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa
    34981 gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg
    35041 atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt
    35101 acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc
    35161 atgaagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat
    35221 atcacgatgt ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc
    35281 cacttcacgc gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc
    35341 cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt tggcccacac
    35401 accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc
    35461 ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt
    35521 tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga
    35581 ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta
    35641 gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat
    35701 cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg
    35761 cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac
    35821 cgccacggtg acgttctggt tagagagacg acggttagtg atgcgtgacc agaggatcgg
    35881 gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc
    35941 atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc
    36001 catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt
    36061 ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc
    36121 cggccctttt tctttcatgg cggttttgaa tttctcttcc atcacatcga aggcctgatc
    36181 ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag
    36241 cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca
    36301 gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg
    36361 ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca
    36421 tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc
    36481 agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat
    36541 ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc
    36601 tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc
    36661 accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg
    36721 ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa
    36781 acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc
    36841 tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcgcctggt agatcccgga
    36901 gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc
    36961 aactgactga tacgccaggc acgcgtcccc gatggtgaac tgcaaatccc agaccctggt
    37021 gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca
    37081 ctcgttattt ttgaaattaa cgctcggata gccacccgtg ccgcgttgca gaatgttgtt
    37141 ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc
    37201 accggaccag ggcggacgga taccgttact ggctttgcgc cagcgaccag tgagtatgcc
    37261 ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga
    37321 gagcaaccaa atagaccgca taattaataa gccattttta tagccgctaa gatattaaag
    37381 gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg tagcaggatc aaaagagtgg
    37441 cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atggttataa acatatttat
    37501 atagcgatta attcaccaga gatatttctg cttgtttgct ccctcatcag aatttaacac
    37561 taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc aaattaataa
    37621 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg
    37681 aaagcaaaaa aatgaagacc attctacctg cagtattgtt tgccgctttc gctaccactt
    37741 ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
    37801 aaacaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
    37861 aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
    37921 ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
    37981 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
    38041 tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
    38101 tgtatacgcc agacaatgta gatgtgaaat accgcgtctg gaaggcggaa gagaaaattg
    38161 acaacgcgga agttcgctaa actgttgtga agtacggcac cccgttggtt ggataaggcg
    38221 gttacgccgc atccaacatc taacgcccga gccggttgcc tgatgcgacg ctggcgcgtc
    38281 ttatcagtat acgccacatc cggcatacca tgccggatgt ggcacatcat tacaacgcaa
    38341 tatccgccac ttctttttga acgggttgcg gtttcaactg cggtttcggc gtactatctg
    38401 ctgcttgcgg tttgtcgtat ttcagcccca gcacttcgct ggtgtactgc aactcgcgtt
    38461 ctgttgccgc tacatcaccg ttcagcttgc gtccataaga cggaacgatc gctttcaacg
    38521 tagactgcca ttgcgggctg gaaacgcgat cgccaaatac tttttccagc agattcaaca
    38581 taatcggcgc ggcggttgac gcccctggcg atgcccccag cagcgccgca atggtccctt
    38641 gctggtcact gaccacttcg gtacccagac gcagtacgcc acctttctcg gcatcacgct
    38701 tgataatctg cacacgctgc cccgcttgcc acaaacgcca gtcctctttt ttcgcttgcg
    38761 gatagtactc tttcaacgct tcaaaacgat cctcttcact caacatcacc tgactcacca
    38821 gatatttcac cagatcgaaa ttatccagcc cgacgtgcat catcggcatc acgttagagg
    38881 tggtggtgga actcatcaga tcccacaatg aaccgttttt gaggaatttg gttgagaagg
    38941 tggcaaatgg cccaaacagc actacgcgtt taccgtccag aacacgggta tcgatatgcg
    39001 gaaccgacat cggtggtgcg ccaacggatg ctttaccgta aactttcgcc agatggcgat
    39061 taaccacgtc tggattttcc gaaacaagga actgtccgcc caccgggaaa ccggcgtagt
    39121 ctttcgcttc cggaatcccc gattcctgta acagcttcag cgccgcaccg cccgcgccga
    39181 taaagacaaa tttggtgcgg atgttctgtg cagtgccgtt tttcagatcg gcaacggtaa
    39241 cggtccaggt gttatcgtca ttacgcttta gggcgcggac ttcgctgctg agttgcagcg
    39301 agaagttaga tttcttctgt aaggaagcaa ttaactggcg ggtgatctca ccatagttca
    39361 catcagtgcc aatttctgtt gcggatcgcg cccttccatc actaacggtg cccactcttt
    39421 gatctgcgcg tgatcttcag agtaacgcat accgcgaaac agcgagcttt gttgcaacgc
    39481 ggcgtaacgg gcgcgcagga aattaacgtt atcctcgccc cagacaaagc tcatatgcgg
    39541 aacggtattg ataaatgaac gcggagtacg cagcacgccg cgctcgacct ggtgcgccca
    39601 gaactggcgg gaaatctgaa atgcttcgtt aatggcgact gctttttcaa tactgatgct
    39661 gccatcggcg ttttgcgggg tgtagttcag ttccatcagt gcagaatgcc cggttccggc
    39721 gttgttccag ccgttcgaac tctcctgcgc gcccccctcc aggcgctcca ccatggtcat
    39781 cgaccattca ggctccagct cgcgtaaata ggtccccaac gtggcgctca taatgccgcc
    39841 gccaatcaac agtacatcag tttcctgctc ctcggacgct ttcgcttttg ccgccatcga
    39901 aacggcatta agccccacgg ccatcgagaa gagcatggca gtcacttttt tcatcttgtt
    39961 aatgccttac ttttagtcgc tttattgcag gtgagatttg cgcggcatca acggtaacac
    40021 ttaagtaaca acatttaaat aatgtttata aattatattc taattttaga aacattataa
    40081 tttcgttgat taattatagg gttattagaa atgaagggat ttttagcata ttgacctgac
    40141 ggcagcagtc cgccaggtca gcgtcgtaat attatgccgt ccgggcaacg gcatcacgcg
    40201 aagcggcatc gcgctcttcg cccgtcagct cgctaagttg cccattgcgc atttccagca
    40261 ggcggtcggc gtggataaag taatgatcat catgactgat agcgaaaata gttttaccca
    40321 tctcctgcat cagcggcagc aacacctgat aaaactcacg acggaagtgt ggatcctgat
    40381 ccgccgccca ttcatccagc aggataatat cgcgttcttc tgccagcgcc agcaacagcg
    40441 gcacgcgttt tttctgccct tttgataact tcaggttaac aatacgcccg ttgcttaact
    40501 caagcttatg agccattttc agctgcgcca gccacttctc aaccagttgc gggttagcgg
    40561 gttgcccctc cggccccagc agttgatcaa acagccagac atcggtaaac actgccgaaa
    40621 acagtttgcg ataatcttcc ggttgttcgc cgctgacagg tttgccatcc agcaagattt
    40681 cgccgctttg tggctgatac aagcccgtca acaacatcgc cagcgtcgat tttccgctac
    40741 cgttgccgcc aatcagaaac agcagctcgc cacgtttgat ggtgagatta atcggaccaa
    40801 cggaaaacgc gttatcctga taagcaaacg tcacgttacg cagctccagc gtttgccagt
    40861 tgggaaacgc ctgcgggcgc ggaaactcag ctttgaaagg cgcgagcgcg aatttgttca
    40921 gcttgttaaa cgccacctgc gccgtcagca gcgtcggcaa tgcgccaacc gccgaaagca
    40981 gcggcgtacg caggaataac agcgtcaacg aataggtcgc agcaacgttg gtatcagccc
    41041 aaccgagact gttcgccatc cagaacacca ggccgatagc gcccagcatc atgatgtttg
    41101 accagttcac ggcactaaga tggaaggtgt ctgcgcgaat aatatgatgg cgatactctt
    41161 gcgcatcggg gatgtagagg ttgttaaaca catactcggc gcgttcccgg ttcagagtca
    41221 gctctttgcg cccttcaagt acagtttgaa aatccgtgta cagcttgtct tcggtttcac
    41281 gtagggtcgc catatgtttg tacacccgcg ccaccagcac aaaaccgccc cagatggtga
    41341 tcgccatcca gatagccgtt accagcaaca ttttgcccga cagcatccac agatacgccg
    41401 atgaaccgat agtgagaatg atcccctgcc ccagttccgg cagacgcaca aaagcaatgg
    41461 tgatattgcg cacatcgctg gttaatcccg ccagcaacga ggcgctaccg agttgttcaa
    41521 tgcgctcgac gtgggtatcc agaatccgct tgataaattc actacgcagt cggtaaacga
    41581 agtgatgccc caaagtggtg agcgccagtt gcgatccgag agtgactgcc atcaacagca
    41641 gcaataatcc cagaaactcc ggtaacacca gcagactggt atccgccgtt tcgataaggc
    41701 gctgattgat aaaagcaatt aagccaatgc ctaatgccgc actggcgagg cttagcgcca
    41761 tcacactgat aaatggccag cgatactgcc gccagacaag tacaagaagt tccatgcaga
    41821 aaacccggac aatgaattac agcccgcagt ttaaacatct tcgcgcgcac agcaataata
    41881 attcttattt ttattctttt ttacctgcct gacggaatgt caggttgtag cggcagtcgg
    41941 tggtgagtgg atgaaacccc gctttcaacg gttgaatacc gtgataaaac agccgcgatt
    42001 caccgcccca ggccaccaaa ttgaaaaatc gcgggtaagc ccagagaaac agaaacaatt
    42061 ggcgcgcgca gatccggttc gtctttatcc tgatgcagcg acagtttcgc gctaggagcg
    42121 tagcggttga taagacaagc atctggctgg aaatctggat agcccgccgc cgtagccgca
    42181 cgttgacata aatcatgaaa gctttgtggc atggcgggcc acggtttatt tgtttgcgga
    42241 tcaatgggcg aatagagata accttgccga tgggtcgtcc agcccagatg cccacagttg
    42301 gtcatcgcca ccgacatggt atatccgcct ggagcgacca tctggcgaaa cggcgactgg
    42361 ctggcaacgt cattaatatc gcggattagt tgctccgcag cattaaaagc aaaacgccgt
    42421 aaaattaccg caccagccgc cagtggctct tgccacggtt cagcatcggc aaacagatcc
    42481 aacattacct ctcctcattt tcagcttcgc ggcgcagcag ttgcgcttta cgcgacacgc
    42541 cccagcggta accggaaagt gtgccatcac cacggaccac tcgatgacag ggtattacga
    42601 tagccagctt gttggcggca caggcgctgg caacggcccg taccgctttc ggtttgccga
    42661 tggcgttagc cagttgctga taactgacgg tttcaccaca aggtatcgtg cgcagtgcct
    42721 gccagacttg ttgctgaaaa gcagtgccgc gaatatccag cggtaacgtc agcggcgtat
    42781 cgcgttgatt gaggctggcg atcacttcac gcacatgttg ctgaaacgtc agatcggcag
    42841 gcgcgttgtc ggcagcggga aacatctgct gcaactcgct gattaatgtc gcgtcatcat
    42901 cgcccagcaa tatcgcgcaa atcccccgct cgctttctgc caccaggcaa cgacccagct
    42961 cacaatcagc cagcgcgtaa cgcaccgcca gattttcgcc accgtgacgg aattgtttag
    43021 ccgtcatgcc cagcgtttca tcggctttgc ggtagtaact gctgctgtcg gggaatccgg
    43081 cgttaagaat agacgtcgtc acgctctccc ctttcgccag cgattcgcgc aaacggcgag
    43141 cgcgccaggc ctgttgccag gctttaggcg tcattccggt agtagcttta aacaaccgat
    43201 gtagatgaaa tggactcatc gccacctggt cggctaaggc ttccagcgtt acaggcgttt
    43261 cctgttccag cagtcgacac gcgtgggtga ttttatccaa ccgatgttgc cggggattgg
    43321 ctttgtctgg ctgacaacgt ttgcaggggc gaaatccggc ggcgagtgcc tcgctggcat
    43381 ttgcgtagaa ggagacattt tcccgcaagg catgtctggc gcggcaagac ggacggcaaa
    43441 agatacccgt ggtgcgcacg gcgaaaacga attcgccgtc ggcatccggg tcgcgggcta
    43501 agacagattg ccagcgttga tcgtcagtta agcatgtggc gtttttcata atcagctccc
    43561 tggttaagga tagcctttag tctgcccggt caccatcacg caaaaaccaa caatcttgcg
    43621 ctttaatttt tttcgctgac aaggaagctt ttaaactgtg gtgacatcca ggttttaaag
    43681 ctatcgcctt ctttggtgat catataaacc gccagccctt cccggcgaac aacctctttg
    43741 gctttctccg gcccgagtac catcaagcca gtatcccagg catcggcttc cagcgccgtc
    43801 ggagcaatca ccgtcacgga taccagattg tgttcgatgg gacgcccggt ttgcggatcg
    43861 ataacatggg aaagacgttt gccgtccagt tcgtaatagt tacggtagct gccagaagtg
    43921 ctaatcccat gaccgttgat atccaccaca gcctgaaccg cgttttcttt atcggttggt
    43981 ttttgaatcg ctacccgcca cggctggcct tcaccgttca taccacggct gttcagcgcg
    44041 ccgcccaccg acaccagata gcgggaaatc ccttcctgct ccatcaagcg tgccaggtga
    44101 tccgccgcat aaccttcacc gacggtggag agatcgacat ataaatccgg caggtctttt
    44161 tgcagatatt gctgatgcga ctgattgatg accgtcaggt gctgtaagcc ggttttggct
    44221 ttcatcgcat cgatctgttc ctggctcgga atttgaaccg gctgttgttc cgggccaaag
    44281 ccccacagat tcaccagcgg cccgacggta atatccatcg caccatcggt ccttgcccca
    44341 atgcgcagcg aggtggtgac gatatcggcc atcgcttcac ttaccggcca cggcgacaaa
    44401 ctttgcgagt cgttaaagcg catcagcgcg gaatcttttt tataggtcga aagcagctga
    44461 tcgtcggcgt ccagctgggt ctgaatcttt tctttaagtt cggcactgcg tttggcatca
    44521 atgcccggga tgctggcacg ccagaaggta cccatggttt tgccttcaag aacggtaact
    44581 tcagtagcgt gggttttggc gggttgtggt ttttgatcgc aaccaacaaa gaagagcgcg
    44641 gcaaccagca gtgccacgcg ggtaaagctt atttccatac gtgattatcc tcatgcgaac
    44701 ggtcgcaaga gtacaccaaa aaactgtgtt tgtacgctga aaacaatgaa aaaagggccc
    44761 gcaggccctt tgttcgatat ccatcgagat tagaactggt aaaccagacc cagagctacg
    44821 atgttatcag tgttgatgcc agcgtcacga gtgaactggt tgtcgtccag caggttgatt
    44881 ttgtagtcaa cgtaggtgga catgtttttg ttgaagtagt aggtcgcacc aacatcaaca
    44941 tatttcagga tatcttcgtc gtcgtagtta cgaccattga tgacacccag gtttttacct
    45001 ttagactgca ggtaagctag ggacggacgc agaccgaagt cgaactggta ctgagcaaca
    45061 gcttcgaagt tctgtgcttt gttcgcccaa cccaagttgc ctacgcgagt tgcgttgtag
    45121 gtctgggtgt actgagcagc taggtagatg ttgttcgcgt cgtatttcag accaccggtg
    45181 taggtttcag cgcggtcgcc attaccgatg taacgctcat cataacggtt cagaccgaag
    45241 ttctggtcat cagtacgttt ggagctggaa actgcagcac cgataccgaa gccttcgtaa
    45301 tcataagtaa tagaaccgcc aacgccgtca ccgttctgac gcagagcttc acgaccgttg
    45361 ttggtcatgc cttcaccttc cgggctaccg tttttgccct gatactgaac agcgaagttc
    45421 agaccgtcaa ccagaccaaa gaagtcggtg ctacggtagg ttgcgaagcc gttaccacgc
    45481 tgctgcatga agttgtcaga accgtaagtg tcaccaccga attctggcag tacgtcggtc
    45541 caggaagtta cgtcgtaaac tacgccgtag ttacgaccgt agtcgaaaga acctacatcc
    45601 tggaatttca gacctgcgaa tgccacacgg gtccaggagt tgttttcgtt ttcagcgctg
    45661 ttgccctgga tctgatattc ccactggccg taaccggtca gctggtcagt aacctgagtt
    45721 tcacctttga agccaagacg catgtaggtc tggtcgccat ctacagactt gtcgtcagag
    45781 aaatagtgca ggccgtctac tttaccgtac agatctaatt tgttgccgtc tttgttgtaa
    45841 acttcagcag cgtttgctgc tcctgctacc agcagagctg ggaccaggag ggacagtact
    45901 ttaactttca tgttattaac cctctgttat atgcctttat ttgctttttt atgccactgc
    45961 atactgatta accctcatta atcagtcggc aagtccattc tccccaaaaa tgcagaataa
    46021 tccaacacga atatgatact aaaactttta agatgtttca tttatcgcta cagatgtttc
    46081 aaaatgtaaa tacaagggaa ctttttaaga ttattgcgga atggcgaaat aagcacctaa
    46141 catcaagcaa taataattca aggttaaaat caataactta ttcttaagta tttgacagca
    46201 ctgaatgtca aaacaaaacc ttcactcgca actagaataa ctcccgctat catcattaac
    46261 tttatttatt accgtcattc atttctgaat gtctgtttac ccctatttca accggatgcc
    46321 tcgcattcgg tttttttacc cttctttaca cacttttcat tattctgtgc taccacagaa
    46381 aaactataac gcttgttaac tatttcacaa ataattaaca tcagcataat ttccagcaat
    46441 ctttgtttat ttacaattaa tttcgttggg ctttttgtag gttatttgta cagcaaaatg
    46501 gcgcttgtac atctatttcc ccccatacag gatgataaat atcacgggag aatagagaat
    46561 catcaatcag gtaagagtct ggaatttcac actgtaccct ttatactgcc ctatcacttc
    46621 gcgaagtttt aacaggtcat aaacacgaat gcgtcagaaa gagacaacgg ccacgacccg
    46681 cttttcactc ctaccgggga gcattacccg cttcttttta ctgttgatca ttgtgttact
    46741 ggtgacgatg ggggtaatgg tacaaagtgc cgttaacgcc tggctgaaag ataaaagtta
    46801 ccagattgtc gacattaccc acgcaatcca aaagcgcgtc gatacctggc gttacgtgac
    46861 caggcagatc tacgacaaca ttgccgcgac gacctccccc tcctccggcg aaggtttaca
    46921 agagacgcgc ctgaaacagg atgtctacta tctggagaaa ccacgccgca aaacggaagc
    46981 gttaatcttt ggctctcacg acaactcaac gcttgagatg actcagcgga tgtccactta
    47041 tctggataca ttgtggggtg cagaaaatgt accgtggtcg atgtattacc tgaatggtca
    47101 ggataacagt ctggtgctga tctcaaccct accccttaaa gatctcacct ccggatttaa
    47161 agaatcgacc gtcagtgaca ttgttgattc acgtcgtgca gaaatgttgc aacaggccaa
    47221 cgccctcgat gaacgcgaaa gtttttctaa catgcgccgc ctggcctggc agaacggtca
    47281 ttactttacc ttgcgtacca cgttcaacca gccgggacat ctggcaacgg tcgtggcttt
    47341 tgatctgccg attaatgatt tgatcccacc gggtatgccg ctggacagtt tccgccttga
    47401 gccagacgcg acggcaacgg gaaacaatga taatgagaaa gaagggacgg atagcgtcag
    47461 tatccacttt aacagtacga agattgaaat ctcctcggca ctcaactcta ccgatatgcg
    47521 cctggtctgg caggttcctt atggcacctt attgctggat acgttgcaaa acattctgct
    47581 gccactgctg ctgaacatcg gtttgctggc gctggcgtta tttggctata ccacattccg
    47641 ccatttctcc agccgcagta cagaaagcgt ccccagcacg gcggtcaata acgaattgcg
    47701 cattttacgg gcaattaatg aagagatagt ctcactgttg ccgctcggcc tgctggttca
    47761 cgatcaggaa tcgaaccgca ctgtcataag taacaaaatt gccgatcatt tgctgccgca
    47821 tctgaatctg caaaacatca ccaccatggc ggaacagcat caggggatta ttcaggcgac
    47881 gatcaataac gagctgtatg agatccgcat gttccgcagc caggtcgcgc cgcgcacaca
    47941 aattctcatt attcgcgatc aggatcgcga agtgctggta aacaagaaac tcaagcaggc
    48001 gcagcgtctg tatgagaaaa accagcaggg gcggatgacc tttatgaaaa acattggcga
    48061 tgcgctgaaa gaacccgcac agtccctggc ggagagtgcg gctaaactca acgccccgga
    48121 aagcaaacaa ctggcgaatc aggcagatgt gctggtgcgg ctggtcgaag aaatacagtt
    48181 agcgaacatg cttgcggatg atagctggaa aagtgagacg gtgctgttct ccgtgcagga
    48241 tttaattgat gaagttgtgc cttcagtgtt gcctgccatc aagcgtaaag gtctgcaact
    48301 gctgattaac aatcatctga aagcacacga tatgcgccgc ggcgatcgcg atgccttacg
    48361 acgtattttg ctgctactga tgcaatatgc cgtgacctca acgcaattgg gaaaaatcac
    48421 ccttgaggtt gatcaggatg agtcctctga agaccgcctg acattccgca ttctggacac
    48481 gggagaaggc gtaagtattc atgaaatgga taatttgcac ttcccgttta tcaaccagac
    48541 ccaaaacgat cgttatggaa aggcggaccc gctggcattc tggctgagcg atcaactggc
    48601 acgtaaactg ggcggtcatt taaacatcaa aacgcgggat gggcttggta cacgctactc
    48661 tgtgcatatc aaaatgctcg ccgctgaccc ggaagttgaa gaggaagaag agcgtttact
    48721 ggatgatgtc tgcgtaatgg tggatgttac ttcggcagaa attcggaata ttgtcactcg
    48781 cctgttagaa aattggggtg caacctgtat cacacccgat gaaagattaa ttagtcaaga
    48841 ttatgatatc tttttaacgg ataatccgtc taatcttact gcctctggct tgcttttaag
    48901 cgatgatgag tctggcgtac gagaaattgg gcctggtcaa ttgtgcgtca acttcaatat
    48961 gagcaacgct atgcaggaag cggtcttaca attaattgaa gtgcaactgg cgcaggaaga
    49021 ggtgacagaa tcgcctctgg gcggagatga aaatgcgcaa ctccatgcca gcggctatta
    49081 tgcgctcttt gtagacacag taccggatga tgttaagagg ctgtatactg aagcagcaac
    49141 cagtgacttt gctgcgttag cccaaacggc tcatcgtctt aaaggcgtat ttgccatgct
    49201 aaatctggta cccggcaagc agttatgtga aacgctggaa catctgattc gtgagaagga
    49261 tgttccaaga atagaaaaat acatcagcga cattgacagt tatgtcaaga gcttgctgta
    49321 gcaaggtagc ctattacatg aacaatatga acgtaattat tgccgatgac catccgatag
    49381 tcttgttcgg tattcgcaaa tcacttgagc aaattgagtg ggtgaatgtt gtcggcgaat
    49441 ttgaagactc tacagcactg atcaacaacc tgccgaaact ggatgcgcat gtgttgatta
    49501 ccgatctctc catgcctggc gataagtacg gcgatggcat taccttaatc aagtacatca
    49561 agcgccattt cccaagcctg tcgatcattg ttctgactat gaacaacaac ccggcgattc
    49621 ttagtgcggt attggatctg gatatcgaag ggatcgtgct gaaacaaggt gcaccgaccg
    49681 atctgccgaa agctctcgcc gcgctgcaga aagggaagaa atttaccccg gaaagcgttt
    49741 ctcgcctgtt ggaaaaaatc agtgctggtg gttacggtga caagcgtctc tcgccaaaag
    49801 agagtgaagt tctgcgcctg tttgcggaag gcctcctagt gaccgagatc gctaaaaagc
    49861 tgaaccgcag tattaaaacc atcagtagcc agaagaaatc tgcgatgatg aagctgggcg
    49921 tcgagaacga tatcgccctg ctgaattatc tctcttctgt gaccttaagt ccggcagata
    49981 aagactaatc atctgtaggc cagataagac gcgttagtgt cttatctggc atttgcaccg
    50041 attgccggat gcggcgtaaa cgccttatcc ggcctacgat tcccattatt tcaacagatt
    50101 acattaaagt aggccagata agacgcgtca gtgtcttatc tggcatttgc actgaatgcc
    50161 ggatgcggcg taaacgcctt atccgtccta cgaatcccgc gatttcctga ccctctcggc
    50221 atataccgtc agcgtctttt ttatcacatc cagcgttacc ggcttcgaga ggcagctgtc
    50281 cataccggac tccagacacc gctgcttctc ttcagccaac gcattagcag ttactccgat
    50341 taccggcaac gtcagtccca actgacgaat gcgttgcgtc aagcggtaac catccatatt
    50401 tggcatgttg acgtcgctaa gcacgatatc aatatgattc ttactaagta cattaagcgc
    50461 atcgacgcca tcattcgcgg ttttacattg atagcccaac gatcccaact gatctgccag
    50521 cagacgtcgg ttaatcggat gatcatccac gaccagaatc atcatatcgt cgttatcgct
    50581 gaccgctttg tccgtcgacg gcagagcgtt agcaggatcg tcgctctcca tctcgatcaa
    50641 ataaatacgc gccaacaatg ccggtagctc atgcggagca gccacactgt gtacccactc
    50701 ccctggcgct ttctccagcg gaataccaat atggcgacga cagaaggtca ctaccgctct
    50761 gccctgccat tttttactca ctacctcgtc agtgattaac acatcttcgg gagtcggttc
    50821 ctgcccttcg tatgttgtaa cgacgatgcc gctgcgctgc aaactggttt ccaggaactg
    50881 acagagcgac gcattgcgga ccgccagcca gcagcgttta ccactcaacc cttccacgcc
    50941 ttttttctgc gggtactgag cgccgtacaa cggaatacgc acggtaaact ggctgcccat
    51001 tcccggttct gaatctaccg agatatcgcc gtccatcatg ctgatcagtt tttcacaaat
    51061 cgccagaccc agaccggtcc cctggaagtt tcgctgtacg cccgttccaa cctggaagaa
    51121 gggatcaaac aagcgcacca cttctttcgc cggaatcccc acgccggtat cgcgaacacg
    51181 gatagagaga taatcgccat cagcgcgaac atgcaaaact atacagccgg tatcggtgaa
    51241 ttttatggcg ttactcaaca ggttggagat gacctgctgt aaacgcatcg ggtcgccatt
    51301 taaggccact ggcacatccg gttcaataaa gcagtacaag cctaactgct tgcgtaccac
    51361 cagcggtaaa tagttggcgg tgatgtggtt catcacttca cgcggtgaaa actcacgcgg
    51421 ttcgatcttc aactgttccg attcaatctt cgagaaatcg agaatatcgc tgataatttt
    51481 caacaacagg ctggaagagt tgttcattgc cgtcaccaga cgatcgacgc ctttcggtaa
    51541 ctctttggtt tgcaacagat ccaggttacc gataatgcca tacagcggcg ttcgcagctc
    51601 atgactgacg gtggcaagga acatcgattt tgactggctc gcctgttccg ctgcttgtgc
    51661 catctcctgc aacgactctt ccatcttcac gcgcgaagaa acatccacca gcacacaaat
    51721 ggccacgttt tcattacgat agcgcgaatg gacgaagctg atttgcagat tggtattgtt
    51781 gctggtcagg acatcaacaa aattgacctg ctgcccacag ataatttgcg tcagtcgttg
    51841 gcggtcctca tgcgtaagca tattgagata ggtatgcgcc agttcgttac ttaaaatatt
    51901 gacgccatca gcggtacgca aaatgcagat acccactggc gcggaggcga caatcttgcg
    51961 attgaactgc tcatgttctt ccagtcgcag ggcgtcgctt tccgccggaa tgaaaatacg
    52021 tcgctcgtac atccgcgcga gagtaaacaa tgcagctccg gcaagcacat tcagcaaaat
    52081 tgcgttaagg atcaacatgc gaatgcgttc cagcacctta tcaaccggca ccgaatacac
    52141 gatgcttagc gatgagggtg gcagattttt cttcagcacc agctcccgga acccttccgt
    52201 atagccaaac caggagcgtt cctgcatcca gcgaggatcg cccttaattt tactttctgg
    52261 tccggtaagc gaaatcaggg tatgaccatt ttcatcaaga atggtaaccc ccatcggcaa
    52321 cgtacccggt aagaaaaagt tctccatccg gatggtctgc tcgacaccca aaagcgcctg
    52381 caaccggttc gccagataaa ctggcgtcaa cgcgtaaaaa tacccgacgc cagggcgcgg
    52441 accttcgctg atccagtaga ggttactgcc gctatcatct tgtggtgcat ttcgatattt
    52501 attgatgcgt tcatgcaacg ctttcaacgc ggtatcgcgt tccactggca tatcacgcag
    52561 accgaaattg gccatgcaga ggttatcact gccgattaaa aatacccggt tgagatcgta
    52621 agccgcagaa aaattatcgc gccagtagcg cataaaccac gccaatgact ccagagaacc
    52681 tcgccaggtg ttactcattg cggaacaatc ggagtcggca aacagcggtt caaacgcagg
    52741 cacatccgcc tgcgtttctc gtccacgcgg ggaaagcaca ccgttttctg ccgataagcg
    52801 attttcggcg atgtacttaa gctctttcat cacatcagag gtgcgttgaa taaagcgctg
    52861 agcctgatcg gaactcagat taaattcctg acgaatttcc gattctcgct gatgtaacgc
    52921 attaacgatg taaaaaacgg atgaaaaagc aatcaacagc cagagcacta acgccaatgc
    52981 tctgaacatg tagcgcgagg ctttcagggt tgtacgaaaa gaagcaaggt atttcaaagg
    53041 ggcgaagctc cgcctcaggt gaccgatgga gtgtggttaa ggtagcggta aaagcgtgtt
    53101 accgcaatgt tctgtcacca ttgcaataat gcgtatcgct tagaaaccat tgccttaacc
    53161 aggaaagccg gagtagatgg acagttctca gtacgaaaaa taagaaatga ccaatctgcc
    53221 gacgcttgtt aatggttata tcctggcgtc ggcagatcgc ccagcagtct tatctgaaaa
    53281 aagagagagc caggttgtgt gaagggatgg atcaccagtc aaaacttttc accagcgtca
    53341 gctcgccagc attacgcatc ggtacaataa atgtttcctg tttctcattg accgatcctt
    53401 catcggtgat cagcgtcagt tgggcggtcg tcaattccgt ttcgctgcgc ccaccatagt
    53461 agttgatata cacctgatag cgcccgtgaa ctggtgctgg catggcgaaa atctcgggtc
    53521 cgtaccccgt cgtgacatcc atatccagtg caccactgtt tttcagcacg gtgttaccgt
    53581 accaggcgtg ttcgccatcg ggcgtgacaa catggagatc gagatcggta ttgtccatat
    53641 cccacgagag aaccagccgt aaacgtgcac gaatagttcc tgtacccggc gttgagtaaa
    53701 actgcatttt ttgccggctt tgcccatccg ggctgatgac ctgcacgctg ttgctgcctt
    53761 cagtgaaaat ataagggcgt gcaaatgaac catcggattc aattctctgc ggcatactgg
    53821 caccgttgac caccagccgg ccctgctgaa ctttacccgc cgcagcataa ttctttattt
    53881 tgccgctgat ctgcgctgag atattttgat catctgccat gttgacggac gacgccgggt
    53941 aattgatcga ttgtgaaaaa ctggcatctt cgccctctgc cggatgccag ccagagagcg
    54001 gtgcgtcaat ctcgacttcc tgcacacctt cactgtgagc gacaggcgaa agtgccacca
    54061 gtaacaacgg aagaaaaatc tttcgcatag cattagtcca ttaataattg tcgggtcagc
    54121 gtttcgatat agttctcatc aagcccggtg ggataaccat caaaggccaa gtgcagatac
    54181 tcatgggtta aatccagccg atcctgaagc gtaaagaagt tacgaataaa cagccgtttc
    54241 tgctgccgat cggtataggg gaaacctgag actaaacgac agaccgcaaa cacgtctggt
    54301 tcgttgtacc ccgtctcgcc ttgtaatata cgccgccact gcggcatttt tttcgccagc
    54361 caggctttcg ctttgggtaa taactggcag gttgagcgtg gagctcccca gcggctaagg
    54421 ctgttgtcgg gataagcaaa cgcgagtatc tggtcgtaac gctccccctg ccccgcctgg
    54481 gccgtggctt gccgccagga aagtgtccct tcggtagcgc gactgccatg ataatgaacc
    54541 ggatcgcctg catagatgag ctcctgcgtc caggcggtca tcgttcgcgc ccccgtggtc
    54601 gctggcgaag cagaaacacg ctgcgtggcg ctgctgccgg ggatggtcag gcaatcacct
    54661 tcccgattgg cgttttgctg taaaaaagta cgtatcgcca cagtcatggc tttcgctgcc
    54721 tcaggtggcg tacttttcgc ctcgcgatcc agaactctgg caacgtactc ttcacgatcg
    54781 agatgcgact gcaatttcag tttgcccttt tcgctcagta aggtcgtctc accgtggctg
    54841 acaaacgtaa tgtggttacc attagtaaac gtcacacgat agcgcccatt aagtacgctg
    54901 ggttttactg ccgtcgtact cttttccgct gtgattttct tcagcggata gcgggcaaac
    54961 agctccacct ctacacattg cccactggca acctgcgtcg gcactggcaa aacccgatta
    55021 agcaccgtcg cataacgggt tagtaccgtt ttgctggtgc cactgccagt aacccacagc
    55081 ggtgtaccat cggttaacca gccagcaaaa ccaccctgac ggatttcctg tttatcatcg
    55141 gcaaaccagc tccaggtttt cacccgtacc cgactaccga gcatggatga gacaccgatt
    55201 ttcgcctcgt ccagcaccac atccagtaag acttcctgag ctttgttttg cgcaggtaac
    55261 gtcgctaatg aatcaagcag cgatttaacc gttaccgagg tttctggctt cagcgttgta
    55321 agagacgcta accacgcagg tgcctgacgc tgttgccagt attgccccca cacatctgcg
    55381 ccaatatgca accgctgcgg cgcaaaatag agcccacagg aacgtaccag cgcggtatcg
    55441 cgcgttatgc tctccccagc ctgacaacaa taaacctctt ccggggagtt tccgcgacac
    55501 tggtagggct gctccggttg atgggtatcc tccagccagg cgtaaacata cagtttccac
    55561 aaactcccta acggcgtggt gagcgagtca ggtaatgctg agactttggt cacgcctgat
    55621 gacgacagct gatacagttg atcgtgctgc gcaccgcgca gcaccagttg taacggcgtc
    55681 tcctccgcaa gcgttggcag agtaaccaac gccaatagcc agacgattct gcgccagttc
    55741 acttatttca cctgcatccc ggtccattca ctccctgccg cgacactttg ctgcgcaggt
    55801 gcataggaac gcacataacg agcaggaggc agaacgaatt gccctttttg cgagaagcgc
    55861 agcaaatggc ggaaagtgac cgttcccgtc agttctttca ccggcaccat ataggccagt
    55921 tcgcccattt cattacgcgc tttttccagc aattgcccct gctgttccgc ggcgttgggt
    55981 ttattgactg aaatgcccca tgttgtgcgc tcaacgtcgg ctcccggcgg gagcggtact
    56041 tccacctgac catagcgcag aactgcatcc tgctcgcagg taagcgtaat ttcatcgaga
    56101 tacagcgcat cgctgtcaat ctcattgctg gtcaccggct gcagagtaaa gctcatctct
    56161 tcttcaccgg ggataagccg atacaactgg cgttcaacgg tcaccggaat gttactttgt
    56221 tgagccgttt ttgccggttc acgccagcgg acctgcacat tttgcggcga taattcgtca
    56281 ccaaaagaga gaatgtccgg cacaccctga ccaacccaac gccagtcttc gccccctcca
    56341 gttaacttat gtttagccca tgcgcccgca ggcgcaggca aaacaactgg aggcattgtc
    56401 gccatatatt tcgccagcca gttcatggcc agcgcgcgct caatagtgga ttgctcagcg
    56461 gtcaaaccat taaaatagcg gccgtctggg tagcgtcacc accgctattg agcagcacaa
    56521 ccgtgtgcgc cagcggctga ttgctgtttg ccgctttatc ccacgcatat tgctgaacct
    56581 gtggcatcac cgtggacttc aactgcgctt ttttcagcaa cgtcatggtt aagacgtttg
    56641 ccaccgcatc tgccagtgga gattccggtg tatcgaggat caggctgtca ttgatatcgc
    56701 ggatgtcttc ctcagagaaa tcttcatctt tagttccgcg ccgggcgata gcttcatccc
    56761 cccctttcaa caacgttttg cacggcagat tcatctcctg tgcccacgcc agcaccagcg
    56821 cccgatgcaa taacggcatg ttatccgcct gctcggcata actgtcgagc atatgctgcc
    56881 agtattccgg ttgttgcgtt acgccgagcg cctggctggc ctgccaatcg gcgtaccatg
    56941 cccatgccgt aaggaaggcg tcaccattgc catcttcacc ccaccaggta aagcgcgctc
    57001 cgggccccgc cagttgcatc agccgcagac ggttatcctg aatcatctga cgaatgtcgt
    57061 tagcggcggc actttgatga tcggcaagcg aacgccaggc gagactgagc gggatcagac
    57121 ggctaccggt gttgatcacc ccaccccacg gttcatcgac taacgcatca agattgttgc
    57181 ggaaaatctc ctgcggcgtt tcactacttt gtagccggat attactcgcc tgctcgggca
    57241 acatcagcgc gttctcgcca ccgccgagca tgacattttt ctgctgttca acgggccagc
    57301 tgttgtccac aaaagacagt tttgtgctaa tgctgtcctg cacttgccca ttttgttgca
    57361 gttctgcact taacaagcca gattgctgaa tattctgcgt cagcgaaata taattcgccc
    57421 ctttgtgcag cgtcagcgtc tggcgcatct cagcgcctgc aaatttagtc accagcgcca
    57481 ccggttcgtt atcctgctga ctgaagataa acagtcctgc cgccggtttg tcgcccacgc
    57541 gatacaccgt tggcatactc cacttcatgt agagattttt ttccgaacgc agataagcac
    57601 gcccctgccc gaccagcccg tcgccgttca tcccacgcgc ggtgatacgc cagcgggtta
    57661 acgaatcagg catcaggaac gtgaaatacg ctttgccttg tttatcggtt gtgagtgacg
    57721 gcatccatgc cgcggtatcc acctcttcac gccgtggacg ttcaggcatt tttactcgcc
    57781 gctcgctgcg gttagtcgcg ccaggcgcaa ccggctcgct ggagagcgcc tggtcatagc
    57841 tgataaacga cagactggag ctggtacgca cattgttacg ccccagcgga tagaaaaatt
    57901 tgccgatatt cggcgcgatt tctggttgca gcgcgtagat catttcatcg accacgccta
    57961 ccgttagctg cgcagaaaca ggtttacctt tcagcgacga ggttaat
//
LOCUS       DAJTXY010000012        56573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTXY010000012 DAJTXY010000000
VERSION     DAJTXY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.012"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..1017)
                     /gene="setA"
                     /locus_tag="OO566_000838"
     CDS             complement(<1..1017)
                     /gene="setA"
                     /locus_tag="OO566_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6604227.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGA"
     gene            complement(1119..1250)
                     /gene="sgrT"
                     /locus_tag="OO566_000839"
     CDS             complement(1119..1250)
                     /gene="sgrT"
                     /locus_tag="OO566_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6604228.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1339..2994
                     /gene="sgrR"
                     /locus_tag="OO566_000840"
     CDS             1339..2994
                     /gene="sgrR"
                     /locus_tag="OO566_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6604229.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3028..3127
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3158..4141
                     /gene="thiB"
                     /locus_tag="OO566_000841"
     CDS             3158..4141
                     /gene="thiB"
                     /locus_tag="OO566_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6604230.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPSGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4117..5727
                     /gene="thiP"
                     /locus_tag="OO566_000842"
     CDS             4117..5727
                     /gene="thiP"
                     /locus_tag="OO566_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6604231.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5711..6409
                     /gene="thiQ"
                     /locus_tag="OO566_000843"
     CDS             5711..6409
                     /gene="thiQ"
                     /locus_tag="OO566_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6604232.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(6523..7287)
                     /locus_tag="OO566_000844"
     CDS             complement(6523..7287)
                     /locus_tag="OO566_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6604233.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7373..8251)
                     /gene="araC"
                     /locus_tag="OO566_000845"
     CDS             complement(7373..8251)
                     /gene="araC"
                     /locus_tag="OO566_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6604234.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8590..10226
                     /gene="araB"
                     /locus_tag="OO566_000846"
                     /pseudo
     CDS             8590..10226
                     /gene="araB"
                     /locus_tag="OO566_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            10237..11739
                     /gene="araA"
                     /locus_tag="OO566_000847"
     CDS             10237..11739
                     /gene="araA"
                     /locus_tag="OO566_000847"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6604235.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            11939..12634
                     /gene="araD"
                     /locus_tag="OO566_000848"
     CDS             11939..12634
                     /gene="araD"
                     /locus_tag="OO566_000848"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6604236.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12709..15060
                     /gene="polB"
                     /locus_tag="OO566_000849"
     CDS             12709..15060
                     /gene="polB"
                     /locus_tag="OO566_000849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6604237.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHNPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15224..18130
                     /gene="rapA"
                     /locus_tag="OO566_000850"
     CDS             15224..18130
                     /gene="rapA"
                     /locus_tag="OO566_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6604238.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAILLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18142..18801
                     /gene="rluA"
                     /locus_tag="OO566_000851"
     CDS             18142..18801
                     /gene="rluA"
                     /locus_tag="OO566_000851"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6604239.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(18918..19733)
                     /gene="djlA"
                     /locus_tag="OO566_000852"
     CDS             complement(18918..19733)
                     /gene="djlA"
                     /locus_tag="OO566_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6604240.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            19988..22342
                     /gene="lptD"
                     /locus_tag="OO566_000853"
     CDS             19988..22342
                     /gene="lptD"
                     /locus_tag="OO566_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6604241.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22395..23681
                     /gene="surA"
                     /locus_tag="OO566_000854"
     CDS             22395..23681
                     /gene="surA"
                     /locus_tag="OO566_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6604242.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRANAYVKILSN"
     gene            23681..24670
                     /gene="pdxA"
                     /locus_tag="OO566_000855"
     CDS             23681..24670
                     /gene="pdxA"
                     /locus_tag="OO566_000855"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6604243.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24667..25488
                     /gene="rsmA"
                     /locus_tag="OO566_000856"
     CDS             24667..25488
                     /gene="rsmA"
                     /locus_tag="OO566_000856"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6604244.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25491..25868
                     /gene="apaG"
                     /locus_tag="OO566_000857"
     CDS             25491..25868
                     /gene="apaG"
                     /locus_tag="OO566_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6604245.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25875..26717
                     /gene="apaH"
                     /locus_tag="OO566_000858"
     CDS             25875..26717
                     /gene="apaH"
                     /locus_tag="OO566_000858"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6604246.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26795..27274)
                     /gene="folA"
                     /locus_tag="OO566_000859"
     CDS             complement(26795..27274)
                     /gene="folA"
                     /locus_tag="OO566_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6604247.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27465..29327)
                     /gene="kefC"
                     /locus_tag="OO566_000860"
     CDS             complement(27465..29327)
                     /gene="kefC"
                     /locus_tag="OO566_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6604248.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29320..29850)
                     /gene="kefF"
                     /locus_tag="OO566_000861"
     CDS             complement(29320..29850)
                     /gene="kefF"
                     /locus_tag="OO566_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6604249.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(29898..31292)
                     /locus_tag="OO566_000862"
     CDS             complement(29898..31292)
                     /locus_tag="OO566_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6604250.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(31351..31638)
                     /gene="fixX"
                     /locus_tag="OO566_000863"
     CDS             complement(31351..31638)
                     /gene="fixX"
                     /locus_tag="OO566_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6604251.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31635..32921)
                     /locus_tag="OO566_000864"
     CDS             complement(31635..32921)
                     /locus_tag="OO566_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6604252.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESSPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(32972..33913)
                     /locus_tag="OO566_000865"
     CDS             complement(32972..33913)
                     /locus_tag="OO566_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6604253.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKEAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(33928..34698)
                     /locus_tag="OO566_000866"
     CDS             complement(33928..34698)
                     /locus_tag="OO566_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6604254.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVNA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35169..36683
                     /gene="caiT"
                     /locus_tag="OO566_000867"
     CDS             35169..36683
                     /gene="caiT"
                     /locus_tag="OO566_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6604255.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFDIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36714..37856
                     /gene="caiA"
                     /locus_tag="OO566_000868"
     CDS             36714..37856
                     /gene="caiA"
                     /locus_tag="OO566_000868"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6604256.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            37985..39202
                     /gene="caiB"
                     /locus_tag="OO566_000869"
     CDS             37985..39202
                     /gene="caiB"
                     /locus_tag="OO566_000869"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6604257.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            39276..40829
                     /gene="caiC"
                     /locus_tag="OO566_000870"
     CDS             39276..40829
                     /gene="caiC"
                     /locus_tag="OO566_000870"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6604258.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            40938..41723
                     /gene="caiD"
                     /locus_tag="OO566_000871"
     CDS             40938..41723
                     /gene="caiD"
                     /locus_tag="OO566_000871"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6604259.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41729..42319
                     /gene="caiE"
                     /locus_tag="OO566_000872"
     CDS             41729..42319
                     /gene="caiE"
                     /locus_tag="OO566_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6604260.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42405..42800)
                     /gene="caiF"
                     /locus_tag="OO566_000873"
     CDS             complement(42405..42800)
                     /gene="caiF"
                     /locus_tag="OO566_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6604261.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(43062..46283)
                     /gene="carB"
                     /locus_tag="OO566_000874"
     CDS             complement(43062..46283)
                     /gene="carB"
                     /locus_tag="OO566_000874"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6604262.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(46301..47449)
                     /gene="carA"
                     /locus_tag="OO566_000875"
     CDS             complement(46301..47449)
                     /gene="carA"
                     /locus_tag="OO566_000875"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6604263.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(47904..48725)
                     /gene="dapB"
                     /locus_tag="OO566_000876"
     CDS             complement(47904..48725)
                     /gene="dapB"
                     /locus_tag="OO566_000876"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6604264.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(48892..49806)
                     /gene="rihC"
                     /locus_tag="OO566_000877"
     CDS             complement(48892..49806)
                     /gene="rihC"
                     /locus_tag="OO566_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6604265.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(49872..50822)
                     /gene="ispH"
                     /locus_tag="OO566_000878"
                     /gene_synonym="lytB"
     CDS             complement(49872..50822)
                     /gene="ispH"
                     /locus_tag="OO566_000878"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6604266.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(50824..51273)
                     /gene="fkpB"
                     /locus_tag="OO566_000879"
     CDS             complement(50824..51273)
                     /gene="fkpB"
                     /locus_tag="OO566_000879"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6604267.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(51360..51854)
                     /gene="lspA"
                     /locus_tag="OO566_000880"
     CDS             complement(51360..51854)
                     /gene="lspA"
                     /locus_tag="OO566_000880"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6604268.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(51854..54670)
                     /gene="ileS"
                     /locus_tag="OO566_000881"
     CDS             complement(51854..54670)
                     /gene="ileS"
                     /locus_tag="OO566_000881"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6604269.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(54713..55654)
                     /gene="ribF"
                     /locus_tag="OO566_000882"
     CDS             complement(54713..55654)
                     /gene="ribF"
                     /locus_tag="OO566_000882"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6604270.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(55662..55880)
                     /locus_tag="OO566_000883"
     CDS             complement(55662..55880)
                     /locus_tag="OO566_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6604271.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            55983..56246
                     /gene="rpsT"
                     /locus_tag="OO566_000884"
     CDS             55983..56246
                     /gene="rpsT"
                     /locus_tag="OO566_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6604272.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        13545 a        15496 c        14680 g        12852 t
ORIGIN      
        1 tgcccccgct cttccaggca ttaaatcctg aaaccatagc atcccaatac ccgcaacaat
       61 gccgataaat acagcgttaa aaagttgcag cgtcatcaac gccatacggc tatgaaagaa
      121 aatcaatcca gtgtaaaaca gcactcctgc caccactgca atgaccatca ttcgccgctt
      181 accataacgt ttgacatagt agccagccag aatcattgct ggtatttcca gtccagctgc
      241 cgtccccatc aggaaacccg ccagtttgtc tggcaatcct aaatcgctac tgatccacaa
      301 cggcatatca ataatgtaca tagtgttgca ggtccacatt aacgtcgagg cgacaaataa
      361 catccgtacg ttactatcct gccagtcacc ttgcattgat aaagcatttt ccgacggcag
      421 ttctacccgc gccacagacg gaagcataaa tgcaatcaat accagactga gtgtgaatat
      481 cccggcggca atcgaaaaca tcactgtaaa gccgtaattc aacgccagca taaaggccag
      541 cggtggaccg attacccatg ccagagaaag ctgcgcacgc atcaccgagc taaacatcac
      601 cacttctcgc gccgagttat ccgcgtattc ccgcgccaga gcaaataact gtggcattgc
      661 cgtattggcc agagatgcca gaagcacacc acaggtgata agcgtcagat aatgacgatt
      721 aaatgcaaac agtagcgcat tgccaatagc catcaaacag caaaatataa tcagttttcg
      781 ccgatcgccc tgactgtcag aacgttttgc caaccagagg cttacgccga tcccagcaat
      841 agcattcacc gtataaaaga gtccgatcca gaaaggttgc gcgccaacct cacgactcag
      901 aaataagctc aatgtaggag cctgtagcgc cccggccacc cccatcataa aagcgaccag
      961 cataaatgcc gcgtaaacac cgttcatacg gcgagccatc gtcattatcc agatcatacg
     1021 ttcccttttt agcgcggcga gaataaaaaa aaccagcagg tataatctgc tggcgggtga
     1081 ttttacacca atactcagtc acacatgatg caggcaagtc aactttcaga attgcggtca
     1141 tcccactgca tcagtccttc aagcatggtt aatcgttgtg ggacgcttaa ccaacgcaac
     1201 cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa actgacgcat ggggcatctc
     1261 cttgcttcat cgttaggaat tattgactta atatagggaa aataaaattg ctgtcttttg
     1321 cgcaggagtt ccccttttat gccatctgct cgtctgcaac aacagttcat ccgcctgtgg
     1381 caatgctgcg agggtaaatc gcaggacaca acgctcaacg aactggcagc gttattgagc
     1441 tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc aggatcgcgg ctggctgacg
     1501 tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga cattcctcta taccgggctg
     1561 gcgcttcagc aacagcgggc ggaagacctg ctggagcagg atcgtatcga ccaactagtg
     1621 cagttggttg gcgacaaagc gactgtgcgg caaatgctgg tttctcatct gggccgcagc
     1681 ttccgccagg ggcggcacat cctgcgcgtg ctctactatc gtccgttgcg taatctgcta
     1741 cctggcagcg cattgcgccg ttccgaaacc catattgccc ggcaaatctt cagttcgcta
     1801 acgcgcataa atgaggaaaa tggggaactg gaagcagaca tcgcccacca ctggcagcaa
     1861 atatcaccgc ttcactggcg tttctttttg cgtccaggag tccattttca tcatggtcgt
     1921 gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa tcaatacgct gccgctctat
     1981 tcgcatatta cggacattgt gtcgccgacg ccctggacgc tggatatcca tctcacgcag
     2041 ccggaccgct ggttgccgtt actgctgggg caagttccgg cgatgatcct gccgtgcgaa
     2101 tgggaaaccc tcagtaactt tgccagccat cctattggca ccggtccgta tgcggtgatt
     2161 cgcaacagca ccaatcaact gaaaattcag gcattcgatg acttcttcgg ttaccgggca
     2221 ttaatcgacg aagttaacgt ctgggttctg ccggaaattg ccgacgagcc agccggtgat
     2281 ctgatgctaa aaggtccaca gggcgaggaa aaagagattg aaagccgcct ggaggaaggt
     2341 tgctactatt tactgttcga cagccgcacc catcgcgggg cgaatcagca agtcagggac
     2401 tgggtaagct atgtgctttc tccaactaat ctggtctatt tcgctgagga acagtaccag
     2461 caactgtggt tcccggctta tggactgctc ccccgttggc accatgcccg caccataaag
     2521 agcgaaaaac cggctggcct ggaaagcctc accctgacct tttatcagga tcacagtgag
     2581 catcgggtga ttgccgggat catgcagcag attctggcaa gtcaccaggt cacgctggaa
     2641 atcaaagaga tcagctacga tcagtggcat gaaggagaga tcgagagcga tatctggctt
     2701 aatagcgcca actttacgct gccgctggat ttttcgctgt tcgcgcactt gtgcgaggtg
     2761 ccgctgctcc aacactgtat tccgatcgac tggcaagccg acgccgcccg ctggcgcaat
     2821 ggcgaaatga acctggcgaa ctggtgccag caactggtcg ccagtaaagc aatggtgccg
     2881 cttatccacc actggctgat cattcaggga caacgcagta tgcgcggcct gcgtatgaat
     2941 accctcggct ggttcgattt taaatcagcg tggtttgcgc caccggatcc atgattgctg
     3001 gtagataaca aaatcactac actaacgccg ttctcaacgg ggtgccacgc aaacgcgtgc
     3061 gctgagaaaa tacccgtcga acctgatccg gataacgccg gcgaagggat ttgaggctcc
     3121 ttctcaagtc ctttgccact cttttttgag gtgcaaagtg ttaaaaaaat gtcttcccct
     3181 gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt ctgactgttt atacctacga
     3241 ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa aaagcttttg aagccgactg
     3301 taactgcgaa ctgaaactgg tggcgctgga agatggcgtt tcgcttctca accgtctacg
     3361 gatggaaggc aaaaatagta aagccgatgt ggtgctgggg ctggataaca atctgttaga
     3421 cgccgccagt aaaaccggac tatttgccaa aagcggtgtg gcagcggatg ccgttaacgt
     3481 tcccagcggc tggaataatg acactttcgt cccgtttgat tacggctatt tcgccttcgt
     3541 ctatgacaag aacaaactga aaaacccgcc gcaaagcctg aaagaattgg ttgagagcga
     3601 tcagaactgg cgggtgattt atcaggatcc gcgtaccagc acgccggggc tgggtctgct
     3661 gttgtggatg caaaaagtct atggcgatga cgccccacaa gcctggcaaa aactggcgaa
     3721 gaaaaccgtc acggtcacca aaggctggag cgaagcctac ggcctgtttt taaaaggtga
     3781 aagcgatctg gtactgagtt acaccacctc tccggcttat cacattctcg aagagaaaaa
     3841 agataactac gccgccgcga actttagcga aggtcactat ctgcaggtgg aagtcgccgc
     3901 ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa ttcctccagt ttatggtttc
     3961 tccggctttt cagaatgcga tcccaaccgg taactggatg tatccggtgg caaatgtcac
     4021 gctgcctgcc gggtttgaac aattgaccaa accggccacc acgctggagt tcacaccagc
     4081 tgaagtggcg gcacaacgtc aggcatggat tagcgaatgg caacgcgccg tcagccgtta
     4141 attcccggct ggttaattcc aggtgtaagc gccgccacgc tggtggtagc ggttgcgctc
     4201 gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta actgggtggc agtctggcag
     4261 gacagctacc tgtggcatgt ggtgcgcttc tccttctggc aggcgtttct ctcggcgcta
     4321 ctctctgtcg tacccgcgat attcctcgcc cgcgccctct atcgcaggcg ctttccgggt
     4381 cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc tcccggtgct agttgctgtt
     4441 ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg catcgctctg ccaatcgctc
     4501 ggtctggagt ggaccttttc gccctacggc ctgcaaggca ttttgctggc gcacgtattt
     4561 tttaatctgc cgatggcgag ccgcttatta ctccaggcac tggaaaacat tcccggcgaa
     4621 caacgtcagc ttgccgccca gcttgggatg cgcggctggc attttttccg cttcgtcgaa
     4681 tggccgtggc tacggcgaca aatcccgccg gttgccgcgc ttatctttat gctctgtttc
     4741 gccagcttcg ccaccgtgct atcgctgggc ggcggtccgc aggcaaccac tatcgagctg
     4801 gctatttatc aggcgctgag ttacgactac gatcctgccc gcgcggcgat gctggcgctg
     4861 ctccagatgg tgtgttgcct tgggctggtg ctgttgagtc agcgactaag taaggccatt
     4921 gcgcccggca ccacgctgct gcaaggctgg cgcgatccgg acgatcgtct gcatagccgc
     4981 atttgcgaca cggtgttaat agtgctggcg ctgctgctgt tgctgccacc gttgctggcg
     5041 gtgatcgtcg atggggtaaa tcgccagttg ccggaagtgc tggcacaacc ggtgctgtgg
     5101 caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag gtgtattgtg cgtagtgctg
     5161 accatgatgc tgctatggag cagtcgcgaa ctgcgggcgc ggcagaaaat gctggcgggt
     5221 caggcgctgg agatgagcgg catgttgatc ctcgccatgc cggggattgt gctggctacc
     5281 ggcttctttt tactgctcaa caacactatc ggcctgccac aatctgctga cggcattgtg
     5341 attttcacca atgcgttaat ggcgatccct tatgcgctga aagtactgga aaacccgatg
     5401 cgcgatatca ccgcccgcta cagtatgttg tgtcagtcgc tgggcattga aggctggtcg
     5461 cgcttaaaag tggtcgagct gcgcgccctg aaacgtccac tggcgcaggc gctggctttt
     5521 gcctgcgtgc tgtcgattgg tgattttggc gtagtggcgt tgttcggtaa cgatgatttc
     5581 cgtaccctgc cgttttatct ctaccagcaa attggctcct atcgcagcca ggacggcgcg
     5641 gtcaccgcgt taattctgct actactctgt tttctgctgt ttaccgtgat tgaaaaacta
     5701 ccggggcgaa atgttaaaac tgactgatat cacctggctt tatcaccatt tgccgatgcg
     5761 ttttagctta acggtagaac gcggcgagca ggtggcaatc ctcgggccaa gcggcgcggg
     5821 taaaagtacc ctgctgaatt tgattgccgg ttttctgacg ccagccagcg gttcgctgac
     5881 tatcgatggc gttgatcaca ccacgacgcc gccgtcacgc cgtccggtgt cgatgctgtt
     5941 tcaggagaac aacctgttca gccacctgac ggtcgcgcag aacatcgggc tggggctaaa
     6001 tccggggttg aaactgaacg cggcacagca ggagaaaatg cacgcgattg cccgtcagat
     6061 ggggattgat aatttaatgg cgcggttacc gggcgagctt tccggcggtc agcgacagcg
     6121 cgtggcgtta gcgcgttgtc tggtgcgcga acagccgatt ttattgctcg atgaaccgtt
     6181 ctctgcgctc gatccggcgt tacgtcagga gatgctgacg ctggtgagca cgagctgcca
     6241 gcagcaaaaa atgacgctat tgatggtgtc gcacagtgtg gaagatgcgg cgcggatcgc
     6301 cacacgctcg gtggtggtcg ccgacgggcg catcgcctgg cagggtaaga ctgaagaatt
     6361 gttgagcggt aaggcgagtg cttcggcaat attggggatt acgggttagt atgcactgtc
     6421 agatgcggcg tgaacacctt atccgaccta cggacagtgc gttttgtagg cctgataaga
     6481 cgcggtcagc gtcgcatgag gcaacccgca caagacaatc ccctaaaccc caaccacttt
     6541 acgcaaaata tcgatataca ccggcatcaa cgggtggcga attaacacca ccagcgccac
     6601 cacgccgatg gcagaaatca acggcgtcag ccacaacaaa cggccgcgcg acaaataatg
     6661 acttaaacga tcggttgctt taccgctgcg ccataaccgc cagcacagcc agccaccaac
     6721 ccacaaaaat accgccgtcg ccagcagcaa ccatttaaac tcgccgctct gcattccggc
     6781 tggaatatcg atcgccgcgc ccgccagaat cccaggcagg aagtaaaacg gtggccacag
     6841 caggcagccg ataatattcg gcgtaatgaa ttttgccacc ggcagatcca gcattcccgc
     6901 caccattggc accagcggac gcgtcgggcc aacaaagcga ccgaccagaa tggtgaacat
     6961 gctgtgttga tgcaacgcat gttcagtttt atcgagtaat gctttgtttt tcttcagaaa
     7021 tgaccagcga tgcaacggct ttttaaaacg ccagcccagc cagaaggaga tccagtcgcc
     7081 cagcagacaa ccgacaatcc ctgccagcca agcgtgccag aaacttaact cgccgctgcc
     7141 aatcagcgct cccagccccg ccatcagcac cgtaccgggt agaatcaaac cgaccagcgc
     7201 cagcgactcc agaaaggcca ccaacaccac cgccatcaat gaatacacgg tagattgggt
     7261 aataaagtgt tccagcaatg cttgcataat gtgcctgtca aatggacgaa gcagggattc
     7321 tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca
     7381 acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg
     7441 ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc
     7501 gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac
     7561 gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca
     7621 gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg
     7681 ccaggtgatc gctgatgtac tgacaagcct cgcgtacacg attatccatc ggtggatgga
     7741 gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca
     7801 gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc
     7861 tgaaatacgg ctggtgcgct tcatccgggc gaaagaaacc cgtattggca aatattgacg
     7921 gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc
     7981 attcgcgagc ctccggatga caaccgtagt gatgaatctc tcctggcggg aacagcaaaa
     8041 tatcacccgg tcggcagaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa
     8101 tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat
     8161 aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg
     8221 gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag
     8281 aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct
     8341 cgctaaccca accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag
     8401 ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt
     8461 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc
     8521 ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt ttttggatgg
     8581 agtgaaacga tggcgattgc aattggcctc gattttggca gtgattctgt gcgagctttg
     8641 gcggtggact gcgccagcgg tgaagagatc gccaccagcg tagagtggta tccccgttgg
     8701 caaaaagggc aattttgtga tgccccgaat aaccagttcc gtcatcatcc gcgtgactac
     8761 attgagtcaa tggaagcggc actgaaaacc gtgcttgcag agcttagcgt cgaacagcgc
     8821 gcagctgtgg tcgggattgg cgttgacagt accggctcga cgcccgcacc gattgatgcc
     8881 gacggtaacg tgctggcgct gcgcccggag tttgccgaaa acccgaacgc gatgttcgta
     8941 ttgtggaaag accacactgc ggttgaagaa gcggaagaga ttacccgttt gtgccacgcg
     9001 ccgggcaacg ttgactactc ccgctatatt ggcggtattt attccagcga atggttctgg
     9061 gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg cgcaatctgc cgcatcgtgg
     9121 attgagctgt gcgactgggt gccagctctg ctttccggta ccacccgccc gcaggatatt
     9181 cgtcgcggac gttgcagcgc cgggcataaa tctctgtggc acgaaagctg gggcggcttg
     9241 ccgccagcca gtttctttga tgagctggac ccgatcctca atcgccattt gccttccccg
     9301 ctgttcactg acacctggac tgccgatatt ccggtgggca ccttatgccc ggaatgggcg
     9361 cagttggcgc aggcgcacag cctaacgcac tggtaaaagt tatcggtact tccacctgcg
     9421 acattctgat tgccgacaaa cagagcgttg gcgagcgggc agttaaaggt atttgcggtc
     9481 aggttgatgg cagcgtggtg cctggattta tcggtctgga agcaggccaa tcggcgtttg
     9541 gtgatatcta cgcctggttt ggtcgcgtac tcggctggcc gctggaacag cttgccgccc
     9601 agcatgcgga actgaaagcg caaatcaacg ccagccagaa acaactgctt ccggcgctga
     9661 ccgaagcatg ggccaaaaat ccgtctctgg atcacctgcc ggtggtgctc gactggttta
     9721 acggtcgtcg cacgccaaac gctaaccaac gcctgaaagg ggtgattacc gatcttaacc
     9781 tcgctaccga cactccgctg ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac
     9841 gcgcaatcat ggagtgcttt accgatcagg ggatcgccgt caataacgtg atggcgctgg
     9901 gcggcatcgc gcggaaaaac caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc
     9961 cgctgcaaat tgttgcctct gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg
    10021 tcgccgcgaa agtgcacgca gacatcccat cagcccagca aaaaatggcc agtgcggtag
    10081 agaaaaccct gcaaccgcgc agcgaacagg cacaacgctt tgaacagctt tatcgccgct
    10141 atcagcaatg ggcgatgagc gccgaacaac actatcttcc aacttccgcc ccggcacagg
    10201 ctgcccaggc cgttgcgact ctataaggac acgataatga cgatttttga taattatgaa
    10261 gtgtggtttg tcattggcag ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc
    10321 caacatgccg agcacgttgt taatgcgctg aatacggaag cgaaactgcc ctgcaaactg
    10381 gtgttgaaac cgctgggcac cacgccggat gaaatcaccg ctatttgccg cgacgcgaat
    10441 tacgacgatc gttgcgctgg tctggtggtg tggctgcaca ccttctcccc ggccaaaatg
    10501 tggatcaacg gcctgaccat gctcaacaaa ccgttgctgc aattccacac ccagttcaac
    10561 gcggcgctgc cgtgggacag tatcgatatg gactttatga acctgaacca gactgcacat
    10621 ggcggtcgcg agttcggctt cattggcgcg cgtatgcgtc agcaacatgc cgtcgttacc
    10681 ggtcactggc aggataaaca agcccatgag cgtatcggct cctggatgcg tcaggcggtc
    10741 tctaaacagg atacccgtca tctgaaagtc tgccgatttg gcgataacat gcgtgaagtg
    10801 gcggtcaccg atggcgataa agttgccgca cagatcaagt tcggtttctc cgtcaatacc
    10861 tgggcggttg gcgatctggt gcaggtggtg aactccatca gcgacggcga tgttaacgcg
    10921 ctggtcgatg agtacgaaag ctgctacacc atgacgcctg ccacacaaat ccacggcgaa
    10981 aaacgacaga acgtgctgga agcggcgcgt attgagctgg ggatgaagcg tttcctggaa
    11041 caaggtggct tccacgcgtt caccaccacc tttgaagatt tgcacggtct gaaacagctt
    11101 ccaggtctgc ccgtacagcg tctgatgcag cagggttacg gctttgcggg cgaaggcgac
    11161 tggaaaaccg ccgccctgct tcgcatcatg aaggtgatgt caaccggtct gcagggcggc
    11221 acctccttta tggaggacta cacctatcac ttcgagaaag gtaatgacct ggtgctcggc
    11281 tcccatatgc tggaagtctg cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt
    11341 cagcatctcg gtattggtgg taaggacgat cctgcccgac tgatcttcaa tacccaaacc
    11401 ggtccagcga ttgtcgccag cctgattgat ctcggcgatc gttaccgtct gctggttaac
    11461 tgtatcgaca cggtgaaaac accgcactcc ctgccgaaac tgccggtggc taatgcactg
    11521 tggaaagcgc aaccggatct gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg
    11581 caccataccg tcttcagcca tgcgctgaac ctcaacgata tgcgccagtt cgccgagatg
    11641 cacgacattg aaatcacggt gattgataac gacacccgcc tgccagcgtt taaagacgca
    11701 ctgcgctgga acgaagtgta ttacggattt cgtcgctaag tagccacatc aggtatgtaa
    11761 cgcctgatgc gacgctgact cgtcttatct ggcctacacg ctgcgatttt gtaggccgga
    11821 taagcaaagc gcatccggca ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct
    11881 acgcgctgcg attttgtaga ccggataagc gaagcgcatc cggcacgaag gagtcaacat
    11941 gttagaagat ctcaaacgcc aggtattaga agccaacctg gcgctgccaa aacacaacct
    12001 ggtcacgctc acatggggca acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat
    12061 caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac gatatggtcg tggttagcat
    12121 cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc tccgacacgc caactcaccg
    12181 gctgctctat caggcattcc cctccattgg cggcattgtg catacgcact cgcgccacgc
    12241 caccatctgg gcgcaggcgg gtcagtcgat tccagcaacc ggcaccaccc acgccgacta
    12301 tttctacggc accattccct gcacccgcaa aatgaccgac gcagaaatca acggcgaata
    12361 tgagtgggaa accggtaacg tcatcgtaga aacctttgaa aaacagggta tcgatgcagc
    12421 gcaaatgccc ggcgttctgg tccattccca cggcccgttt gcatggggca aaaatgccga
    12481 agatgcggtg cataacgcca tcgtgctgga agaggtcgct tatatgggga tattctgccg
    12541 tcagttagcg ccgcagttac cggatatgca gcaaacgctg ctggataaac actatctgcg
    12601 taagcatggc gcgaaggcat attacgggca gtaatgactg tataaaacca cagccaatca
    12661 aacgaaacca ggctatactc aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca
    12721 ggttttatct taacccgaca ctggcgggac accccgcaag ggacagaagt ctccttctgg
    12781 ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt
    12841 attcacgccg atcaggttcc ccgcgctcag catattttgc agggtgaaca aggctttcgt
    12901 ctgacaccgc tggcgttaaa ggattttcac cgccagccgg tgtatggcct ttactgtcgc
    12961 gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg aaggtggcgt taccgtctac
    13021 gaggccgatg tgcgcccgcc agaacgctat ctgatggagc ggtttatcac ctcaccggtg
    13081 tgggtcgagg gtgatatgca caatggcact atcgttaatg cccgtctgaa accgcatccc
    13141 gactatcgtc cgccgctcaa gtgggtttct atagatattg aaaccacccg ccacggtgag
    13201 ctgtactgca tcggcctgga aggctgcggg cagcgcatcg tttatatgct ggggccggag
    13261 aatggcgacg cctcctcgct tgatttcgaa ctggaatacg tcgccagccg cccgctgctg
    13321 ctggaaaaac tcaacgcctg gtttgcaaac cacaatcccg atgtgatcat cggctggaac
    13381 gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg agcgttaccg tcttccgctg
    13441 cgtcttgggc gcgataatag cgagctggag tggcgcgagc acggctttaa aaacggcgtc
    13501 ttttttgccc aggctaaagg tcggctaatt atcgacggta tcgaggcgct gaaatccgcg
    13561 ttctggaatt tctcttcatt ctcgctggaa actgtcgctc aggagctatt aggcgaagga
    13621 aaatctatcg ataacccgtg ggttcgaatg gacgaaattg accgccgttt cgccgaagat
    13681 aaacctgcgc tggcaactta taacctgaaa gattgcgagc tggtgacgca gatcttccac
    13741 aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg tgaacggcct gccggtggat
    13801 cggcacggcg gttcggtggc agcgtttggt catctctatt ttccgcgaat gcatcgcgct
    13861 ggttatgtcg cgcctaatct cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg
    13921 atggattcac ggccagggct ttatgattca gtgctggtgc tggactataa aagcctgtac
    13981 ccgtcgatca tccgcacctt tctgattgat cccgtcgggc tggttgaagg catggcgcaa
    14041 cctgatccag agcacagtac cgaaggtttt ctcgatgcct ggttctcacg ggagaaacat
    14101 tgcctgccgg agattgtgac taacatctgg cacgggcgcg atgaagccaa acgtcagggt
    14161 aataagccgc tttcgcaggc gctgaagatc attatgaatg ccttttatgg cgtgctcggc
    14221 accaccgcct gccgcttctt cgatccgcgc ctggcatcgt cgatcaccat gcgtggtcat
    14281 cagatcatgc ggcagaccaa agcattgatt gaagcgcagg gctacgacgt tatctacgga
    14341 gataccgact cgacttttgt ctggctaaaa ggtgcacatt cggaagaaga ggcggcgaaa
    14401 atcggtcgtg cactggtgca gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa
    14461 cggctgacca gcgcattaga actggagtat gaaactcatt tctgccgttt tcttatgcca
    14521 accattcgcg gagctgatac cggcagtaaa aagcgctacg ccgggctgat tcaggagggc
    14581 gacaagcagc ggatggtgtt taaagggctg gaaaccgtgc gcaccgactg gacgccgctg
    14641 gcccagcagt ttcagcagga gctgtacctg cgtatcttcc gcaacgagcc atatcaggag
    14701 tacgtccgcc aaaccatcga caaactgatg gcgggtgaac tggatgcgcg gctggtttac
    14761 cgtaaacgcc ttcgccgtcc gctgagcgaa tatcagcgta atgttccgcc tcatgtacgc
    14821 gccgctcgcc ttgccgatga agaaaatcaa aagcgcggtc gccccttgca atatcagaat
    14881 cgcggcacca ttaagtacgt atggaccacc aacggcccgg agccgctgga ctaccaacgt
    14941 tcaccactgg attacgaaca ctatctgacc cgccagctac aacccgtggc ggagggaata
    15001 ctccctttta ttgaggataa ttttgctaca cttatgaccg ggcaacttgg gctattttga
    15061 gcaaaaaaag agttcgccag ataccatttt gatgcgtgac gaatgctttg ccatccagta
    15121 ccatagcgcc ctttccattc ctggacctga ataacaccac tacctcataa gcacggtagc
    15181 gggtggttat tgcctgcaat taaagatata gagccgaaca catatgcctt ttacacttgg
    15241 tcaacgctgg atcagcgata cagaaagcga attgggactt ggaaccgttg tcgcggtgga
    15301 tgcgcgaact gtcactttac ttttcccatc tactggtgaa aaccgtctgt acgcacgcag
    15361 tgattccccc gtgacccgcg tgatgttcaa ccctggtgat accattacca gccatgacgg
    15421 ctggcagatg caagtcgaag aagtaaaaga agaaaatggc ttgctgacct atatcggtac
    15481 tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa gttttccttg atagcaaact
    15541 ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag attgaccgta tggaccgctt
    15601 tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg
    15661 cctgcgcggt cagcgtacca gcctgatccc gcatcagctc aacatcgctc atgatgttgg
    15721 tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg ggtttaggga aaaccattga
    15781 agccgggatg atcctgcatc agcaactgct ctctggcgct gctgaacgtg tgctgattat
    15841 cgtcccggaa accttacagc atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg
    15901 ctttgctctg tttgatgatg agcgttatgc cgaagctcag cacgatgctt acaacccgtt
    15961 tgacaccgaa cagctggtga tttgctcgct ggattttgcc cgtcgtagca aacagcgcct
    16021 ggaacatctc tgtgaagccg aatgggacct gctggtggtc gatgaagcgc atcacctggt
    16081 gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt gaacaactgg cagagcacgt
    16141 gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg gggatggaaa gccacttcgc
    16201 ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca
    16261 gaaaaattat cgtccggttg cggacgccgt tgccattctg ctggcaggta acaaactgag
    16321 caatgacgaa ctgaacatgc tcggcgagat gatcggcgag caggatatcg agccgctgtt
    16381 gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat
    16441 gctgatggat cgccacggca ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa
    16501 aggattcccg aaacgcgagc tgcacaccat taagctgccg ctaccgacgc agtatcagac
    16561 ggctattaaa gtctccggca ttatgggcgc acgtaaaagt gcggaagatc gtgctcgcga
    16621 tatgctctac ccggagcgta tttatcagga atttgaaggt gataacgcca cctggtggaa
    16681 cttcgatccg cgcgttgagt ggctgatggg ctacctgacc agccatcgct ctcagaaagt
    16741 gctggtgatc tgcgccaaag ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg
    16801 tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg attatcgaac gtgaccgcgc
    16861 tgccgcctgg tttgccgaag aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg
    16921 ttctgaagga cgtaactttc agttcgccag ccacatggtg atgtttgacc tgccattcaa
    16981 cccggatcta ctggagcagc gtattggtcg tctggatcgt atcggccagg cgcacgatat
    17041 tcagatccat gtgccttatc tggagaaaac cgctcagtcg gtgctggtgc gctggtatca
    17101 cgaaggtctg gatgcatttg agcacacctg cccgaccgga cgcactattt acgatagcgt
    17161 atacaacgat ctgattaact atctggcttc accggatcaa accgaaggct ttgacgatct
    17221 gatcaaaagc tgccgcgagc aacatgaagc gctgaaagca cagctggaac agggtcgtga
    17281 ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat
    17341 tgaagagcag gatgacgata ccaacctgat cgccttcgcc atgaacctgt tcgatattat
    17401 cggtatcaat caggacgatc gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat
    17461 gctggtgccg gacttccctg gcctgtcgga agatggcatc accatcacct ttgatcgtga
    17521 agtggcgctg gcgcgtgaag atgcacagtt tattacctgg gagcatccgc tgatccgcaa
    17581 cggtctggat ctgatccttt ctggcgatac cggtagcagc acgatttcac tgttaaaaaa
    17641 caaagcgttg ccggtaggta cgctgttggt ggaactgatt tatgtggttg aagcccaggc
    17701 tccgaagcag ttgcagctca accgcttcct gccaccgacg ccggtacgta tgctgctgga
    17761 taaaaacggc aacaacctgg cggcgcaggt agagtttgaa acctttaacc gccagcttaa
    17821 cgcggttaac cgtcacaccg gcagcaaact ggttaacacc gtgcagcagg atgttcacgc
    17881 tatccttcaa ctgggtgaag cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc
    17941 gcgtaacgaa gccgacgaaa aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc
    18001 agtgaacccg aacattcgtg acgacgaact gaccgccatt gagagcaacc gtcagcaggt
    18061 aatggaaagc ctggatcagg caggttggcg tctggatgcc ctgcgtttga tcgttgtaac
    18121 gcatcagtaa cggagccgaa aatggggatg gaaaactaca atccaccgca ggaaccctgg
    18181 ttggttatcc tgtatcagga tgaccatatt atggtggtca acaagccgag cggtttgttg
    18241 tcagtgccgg gtcgtctgga agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat
    18301 tatccgcagg cagaatcggt gcatcgtctg gatatggcta ccagcggcgt gattgtagtg
    18361 gcgctgacca aagccgcgga gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa
    18421 aagcagtatg tggcccgcgt ctgggggcat ccatcccccg cagaaggtct ggtggatctg
    18481 ccgctgattt gcgactggcc aaaccgcccg aaacagaaag tctgttacga aacgggtaaa
    18541 cctgcgcaga cggaatatga agtggtggag tatgcggcgg ataacacggc aagagcggtg
    18601 ttaaaaccga ttaccgggcg ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat
    18661 ccgattctcg gcgatcgttt ttatgcatca ccagaagcga gagcgatggc accgcgtttg
    18721 ttgctacatg cagagatgtt gacgattacc catccggcgt atggcaatag tatgacgttt
    18781 aaagcgccag cggattttta atcgtcacgc tggcgcagtt cggtaggcgg ataaggcgtt
    18841 cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg acgcgggccg cgtcttatca
    18901 ggcctacagt ttaaaactta tttaaaccct ttctgctgct ttatcagctc atatgcctgc
    18961 tgaatttcct gcgctttctg cttcgccatc tccatcatct caggcggcaa acctttcgcc
    19021 accagcttat cgggatggtg ttcactcatc agcttacggt aggcacgttt gatggtggtc
    19081 gcatcatccg tcggcttcac gcccagcaca ttacaggcat cttccagcgt cgggccacgc
    19141 tgcgcttgct gccagttacc accgccagat tgctgctgat aaccgccgcc aaactgtgca
    19201 ccgccctgca tcatgcgcaa aaactggtca aactgggcgc gggagatccc taattcttca
    19261 gcaatgacat acagcaccgc ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc
    19321 tgaatctgga tctccagaaa catacgaatt aagtcaaaac gaccaaagca gacactgcga
    19381 aactggcgca tcttttcgcg cagcgggtaa ttatctgatt ttcccacccg gaacgcgttt
    19441 tgcgccgcag tacgggaagc gccatgaagg ttcattcggt ccatcaactg gctggcgata
    19501 tgaatatcag cctccgtgac gcgacctttg gatttggtta aatgccccat cacttcaaaa
    19561 gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc cattttacgg
    19621 ctacgggctt tatcaaacat atggccaatt aacaggccta acactacgcc ccaaaagccg
    19681 ccgcccatca gtaaggccac ggccacgcca atgatttttc cccaatactg catatattcc
    19741 ccaaatcgac acacggatat cagggctatc tcccacaata taaaggtgct tttaccgttt
    19801 tccggctgcg gtcaattgtg ggacatcgcc tataatttgc attatcatac ctgtcattca
    19861 atgccgtgcc taacaccact gacgctattc ggacaggatt aacactagcg tagagatgac
    19921 aagtacgtta gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa
    19981 tcaacgtatg aaaaaacgta tccccactct cctggccacc atgattgcca ccgcccttta
    20041 tagtcaacag ggactggcag ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta
    20101 tgaccgtcct ctggtacagg gcgataccaa tgacttaccc gtgactatca atgctgacca
    20161 cgcgaaaggg gactacccgg atgacgccgt gtttactggc agcgtggata tcatgcaggg
    20221 taacagccgt ctgcaggccg acgaagtgca gctccatcaa aaagaggcac caggacaacc
    20281 ggagccggta cgtaccgttg atgcgctcgg taatgtccat tacgacgata accaggtgat
    20341 cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa gataccaacg tctgggaagg
    20401 tgattaccag atggtgggtc gccagggtcg cggtaaagcg gacctgatga aacaacgtgg
    20461 cgaaaaccgc tataccattc tggataacgg tagctttacc tcctgtctgc cgggttctga
    20521 cacctggagc gtggtaggta gcgaaattat tcatgaccgc gaagaacaag ttgcggagat
    20581 ctggaacgcc cgctttaagg tgggtccggt accgatcttt tatagcccct atttgcagtt
    20641 gccggtgggt gacaaacgtc gctctggttt cttgatcccg aacgccaagt acaccaccac
    20701 caactacttt gagttctacc tgccatatta ctggaacatc gcgccaaata tggatgccac
    20761 catcacgccg cattatatgc atcgtcgtgg caacatcatg tgggagaacg aattccgcta
    20821 cctctcccag gcgggcgctg gcttgatgga actggactat ctgccttcag ataaagtcta
    20881 tgaagatgaa cacccgaacg atgacagttc acgtcgttgg ttattctact ggaaccactc
    20941 cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca gcgatcctag
    21001 ctacttcaat gatttcgata acaagtacgg ttccagtact gacggctacg caacgcaaaa
    21061 attcagcgtt ggctatgcgg tgcaaaactt caatgccacc gtttcaacca agcagttcca
    21121 ggttttcagc gaacagaaca ccagtagcta ctcggcagag ccgcagttag acgttaatta
    21181 ctaccagaat gatgttggtc cgtttgatac gcgtatttac ggccaggcag tgcactttgt
    21241 taacaccaga gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt
    21301 gccgctctct aataactggg gcagcatcaa taccgaagcg aagtttctgg caacccatta
    21361 tcagcaaacc aatcttgact ggtataactc cagaaacacg accaagctgg acgaatccgt
    21421 taaccgcgta atgccgcaat tcaaagttga cggcaaaatg gtctttgaac gcgatatgga
    21481 aatgctggct ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc
    21541 gtatcgcgat cagagcgaca tctataacta cgactcgtct ctgctgcaat ctgactactc
    21601 tggcctgttc cgggaccgga cttacggcgg tcttgaccgt attgcctccg ctaaccaggt
    21661 gacgaccggt gtcacatctc gcatatatga tgatgctgcc gttgaacgtt ttaatatttc
    21721 cgttggtcaa atctactatt tcacggagtc tcgcactggc gatgacaaca taacatggga
    21781 gaatgacgac aaaacgggct cactggtgtg ggcaggcgac acctactggc gtatctccga
    21841 gcgttgggga ttgcgtggcg ggattcagta cgatacacgt ctggataacg tagcgaccag
    21901 taactccagc attgaatacc gtcgggatga agaccgtctg gtacagctga attaccatta
    21961 cgccagcccg gaatatattc aggctacgct gcctaagtac tattccactg ctgagcaata
    22021 taagaatggt atttcgcagg taggtgctgt cgccagcagg ccaattgccg atcgttggtc
    22081 cattgttggg gcctactact acgacaccaa tgcgaacaag caagccgact ctatgttagg
    22141 tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg
    22201 ttgggataac gataaacaac atgcggtata tgacaacgca atcggcttta acatcgaact
    22261 tcgcggcctg agctccaact acggtctggg tacgcaagag atgctgcgtt cgaacattct
    22321 gccgtatcaa aacactttgt gatttgttga tttaccacgt aatccgcagt gcggttaatt
    22381 gaaatggaaa aagtatgaag aactggaaaa cgctgcttct cggtatcgcc atgatcgcga
    22441 ataccagttt cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc aataacggcg
    22501 tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt aaaactgaac gctgcgcagg
    22561 caaggcagca gcttcctgat gacgcgacgc tgcgccacca aatcatggaa cgtttgatca
    22621 tggatcaaat catcctgcag atggggcaga aaatgggagt gaaaatctcc gatgagcagc
    22681 tggatcaggc gattgctaac atcgcgaaac agaacaacat gacgctggat cagatgcgca
    22741 gccgtctggc ttacgatggt ctgaactaca acacttatcg taaccagatc cgcaaagaga
    22801 tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg
    22861 aagtcgaatc cctggcgcag caggtgggta accaaaacga cgccagcact gagctgaacc
    22921 tgagccacat cctgatcccg ctgccggaaa acccgacctc tgatcaggtg aacgaagcgg
    22981 aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg
    23041 cgattgctca ttctgccgac cagcaggcgc tgaacggcgg ccagatgggg tggggccgta
    23101 ttcaggagtt gccggggatc ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg
    23161 ttggcccgat tcgttccggc gttggcttcc atattctgaa agttaacgac ctgcgcggcg
    23221 aaagcaaaaa tatctcggtg accgaagttc atgctcgcca tattctgctg aaaccgtcgc
    23281 cgatcatgac tgacgaacag gcccgcgtga aactggagca aattgctgct gatatcaaga
    23341 gtggtaaaac gacttttgct gccgcagcga aagagttctc gcaggatcca ggttctgcta
    23401 accagggcgg tgatctcggc tgggctacac cagatatttt cgatccagcc ttccgtgacg
    23461 ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca ttcggctggc
    23521 atttaatcga actgctggat acccgtaatg tcgataaaac cgacgctgcg cagaaagatc
    23581 gtgcataccg catgctgatg aaccgtaagt tctcggaaga agcagcaagc tggatgcagg
    23641 aacaacgtgc caacgcctac gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt
    23701 tgtgatcact cccggcgagc ccgccgggat tggcccggac ttagttgtcc agcttgcaca
    23761 gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc actctcctta ccgaccgggc
    23821 agcgatgctc ggtttgccgc tcaccctccg cccttattcc cccaactccc ctgcacaacc
    23881 gcaaactacg ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg tcactgcggg
    23941 gcagttagcg attgaaaatg ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg
    24001 ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa
    24061 cgacgcaagc attccgttta ccggtcatac cgagtttttc gaagagcgtt cgcaggcgaa
    24121 aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt
    24181 accgctgcgc gatatcgcag atgctatcac ccctgcactt ttgcacgaag tgattgctat
    24241 tttgcatcac gatttgcgta caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg
    24301 gctgaatccg cacgcgggcg aaggcggtca tatgggtacg gaagagatag acaccattat
    24361 tccggtgctc gacgagctgc gggcgcaggg aatgaaactc aacgggccgc tgcctgccga
    24421 taccctgttt cagccgaaat atctcgataa cgccgacgcc gtgctggcga tgtaccacga
    24481 tcagggtctt cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg
    24541 cctgcccttt attcgcacat cagtggacca cggcaccgcg cttgaactgg cgggacgtgg
    24601 cgaagccgat gtcggcagtt ttattacggc gcttaatctc gccatcaaaa tgattgttaa
    24661 cacccaatga ataatcgagt ccaccagggc cacttagccc gtaaacgctt cgggcaaaac
    24721 tttctcaacg atcagttcgt gatcgacagc attgtttctg ccattaaccc gcaaaagggc
    24781 caggcaatgg tcgaaatcgg ccccggtctt gcggcattga ccgagccggt cggcgaacgt
    24841 ctggaccagc tgacggtcat cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat
    24901 ccattcttag gcccgaaact gacgatttat cagcaggatg cgatgacctt taactttggc
    24961 gaactggccg agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc ttataacatc
    25021 tccacgccgt tgatgttcca tctgtttagc tatactgatg ccattgccga catgcacttt
    25081 atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt
    25141 cgattaagcg tcatggcgca atactattgc aacgtgatcc cggtactgga agttccgccg
    25201 tcagccttta caccaccacc caaagtggat tccgccgtcg tgcgcctggt ccctcatgca
    25261 acgatgcctc acccggttaa agatgttcgc gtgttgagcc gcatcaccac cgaagccttt
    25321 aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta
    25381 accggaatgg ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc
    25441 cagatggcga actatctggc ggagaacgcg cctttgcagg agagttaacg atgatcaatt
    25501 cgccccgagt gtgtattcag gttcaaagcg tctacattga ggcacaatct tcacctgata
    25561 atgaacgtta cgtttttgct tataccgtaa ccatacgcaa tctggggcga gcgccagtgc
    25621 agttgttggg gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg
    25681 gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg cgaagagtac cagtacacca
    25741 gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg tcactacgaa atgatcgatg
    25801 aaaatggcgt ccctttcagc atcgacattc ccgtattccg actcgccgtt cccacactca
    25861 ttcattaaaa gaatatggcg acatacctta ttggcgacgt tcatggttgt tacgatgaac
    25921 tgatcgcatt gctgcataaa gtagaattta cccccgggaa agataccctc tggctgacgg
    25981 gcgatctggt cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg aaatccttag
    26041 gcgacagcgt acgtctggtg ctgggcaatc acgatctgca tctgctggcg gtatttgccg
    26101 ggatcagccg caataaaccg aaagatcgcc tgacaccgct gctggaagcg ccggatgccg
    26161 acgagctgct taactggctg cgtcgccagc ctctgctgca aatcgacgaa gagaaaaagc
    26221 tggtgatggc ccacgcaggg atcacgccgc agtgggatct gcagaccgcc aaagagtgcg
    26281 cgcgcgatgt agaagcggtg ctgtcgagtg actcctatcc cttctttctt gacgctatgt
    26341 acggcgatat gccaaataac tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta
    26401 tcaccaacgc ctttacccgt atgcgttttt gcttcccgaa cggtcaactg gatatgtaca
    26461 gcaaagaatc gccggaagaa gcccctgccc cactgaaacc gtggtttgcg attcctggcc
    26521 ctgtcgctga agaatacagc atcgcctttg gtcactgggc atcgctggaa ggcaaaggta
    26581 cgccggaagg tatttacgcg ctggataccg gctgctgctg gggtgggtca ttaacctgcc
    26641 tgcgctggga agataaacag tattttgtcc agccgtcgaa ccggcataag gatttgggcg
    26701 aagcggcggc gtcttaaaca cagcctgata caggaaggcc ggataagacg cgaccggcgt
    26761 cgcatccggc actagccgta aattctatac aaacttaccg ccgctccaga atctcaaagc
    26821 aatagctgtg agagttctgc gcatcggcgt cgtggaattc gctgaatacc gattcccagt
    26881 catccggctc gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca
    26941 gatacagttt ttgcgctttt ggcaggaact gctcataaac gcgaccgccg ccaatcacca
    27001 tgatttctgg tacgtcacca cacgccgcaa tggcttcatc caccgatttc acccacgtta
    27061 cgcgatcgtc cgtacccggt tgactgctga ggataatatt tttgcgtcct ggcaacggac
    27121 gaccgattga ttcccaagta tggcggccca taatcacggg tttatttaag gtgttgcgtt
    27181 taaaccaggc gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc
    27241 gatctaccgc taacgccgca atcagactga tcattgagat ttcccgataa aaaaattgtc
    27301 gccactatac gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat
    27361 tttatattct gctggcgagt ccacgctctc ccccaggact cgccgcatta caatgaaaca
    27421 aaaacaaaca gttagctgta aagtgtgatt tacgtcactc tttattaaga tgagggtttc
    27481 gtttccggtt catccgccat gttgccggta tgtttacctt cttctgttcc ctgccagcca
    27541 tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtga tgatctcact taacatcgcg
    27601 ctggtgcgtt tatagaccgc cgcgcgggct ttggtgtcat tctcaaccat tgccatctct
    27661 tccaccatct gaatattaaa gcggcggaac acatcggcac gttctcgcgc ttcatacggc
    27721 cccagaccta aactttccag cgccagacgc ccggttttca gcgcaccttc aaaggtttca
    27781 cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt agtgatcgac atcgcgggcg
    27841 cgggcaataa tctgcaaatg cgggaaatgt tctttcacca tctccgtcag ttgcaggttg
    27901 gtttgcggat cgtcaatagc gttaatcagc acttccgctt tcgccgctcc ggcagattcc
    27961 agtaaatcca tccgcgtggc atcgccataa aacactttca taccaaattt acgcagcgtt
    28021 tcgatatggt ccggatcgtg atcgagcacc accattttca ccccgctgga gagcagtaaa
    28081 cgtccggtaa tctgtccaaa acgaccgaat ccggcgataa tcacgcgcgg ctgttcttca
    28141 tcgatctcat cggcttcacg cgcctcctcc gtagaagatt gctcaaggcg attgaggatc
    28201 accagcagaa tcggcgttgc tgccatcgac agcgccaccg ccagggtcag cgatttcgcc
    28261 cactccggct ccagcacatt cgccatctgc gccgcaccaa ataccacaaa ggcaaactca
    28321 ctgccctgcc ctaacaacac cgcaaaccag cgacgctgtt tatttggcac ctgcaacggt
    28381 cgggcaatca gccacagcat ggcgattttg atgatgagga aaccgagcag caaaatgaca
    28441 atgcgcaatg ggttttcaat cagcgtgcca aagtctatcg acatgccaac accgatgaaa
    28501 aacagcccca acagcaaacc tttaaacggt tcgatatcgc tttccaacgc atgacggtat
    28561 tccgagcttg ccagcagtac gcccgccaga aacgcgccca tcgccatcga caagccgacc
    28621 tcttccagca gcaaaccaaa gccaaacaca aggaataacg ccacggcact aaacacttcc
    28681 cgcaagccag agcgggcaac aaaacgcagc gccggacgcg taacatagcg ccccagcaat
    28741 accaccagta ccagcgcacc cgccactttt aacgccgaga gagcaaatgc gcccatcgtc
    28801 gtcgaggcac tgctcgccgc cagtagcggg atcatcgcca ccagcgggat tgccgcgata
    28861 tcctggaaca gcagcactgc aaaggcactg cggcccattt gcgtcaccat cagattgcgt
    28921 tcattcatcg cctgcatggc aatcgccgtt gatgagagcg ccagcgtcat accgatcaat
    28981 tccgcgacct gccagcgcag cccaagtaac atacagaaca gccccagcag gccaccgcaa
    29041 atcaccatct gcaatgcgcc accgccgaac actgccgcac gcagcttcca cagcctttgt
    29101 ggatcgagtt cgaggccgat aataaacagc atcagcacca ccccaatctc ggcaaagtgc
    29161 agaatagatt cggcatcggt caccagtcgc agcccccacg ggccaataat gcagccggcg
    29221 atcaggtagc caagtaccga tcccagacca agacgtaccg caatgggtac aatcagcgcc
    29281 gccgatccga ggtagatcag cgcctgaacc agcgtatgac tatccatgat gggcctcctg
    29341 ccattccagc agacgttgct tatagtggcg cgcctgccct tcgagggttt cgtcgtcaca
    29401 aataaaggtg cagtgcatgg caaacggtgg cagccagttc agcccgcagt agattgccgt
    29461 cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt caaaatggct
    29521 ttccccgccg ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc
    29581 gtgaccgtaa gcccagccgt gcgacaaaac tttatcgatc caaagtttaa gaagcggagg
    29641 aatgctgtac cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag agagcgcctc
    29701 ctgctcggcg gcaatatcga tattgaagtc aggatagagt tgataaagag agcgaatttc
    29761 gacgccttcc agcgtccttg cctgttcaag catccgttta ttcgcatggg aatgatgcgg
    29821 atacggatgc gcataaatga tgaggatcat agttattagc ccgcgtgttt acgcctttgt
    29881 tttcccacaa agtgtagtca tatattcacg cggcttatag tcaataatat tgattcagaa
    29941 aagagagaaa tctgaacaaa taatcccatt ctctgcccgc ggatcgtcat attgctggtc
    30001 tgcgccagtg acattcctcg agtctccggg gcaaacgcta cggaaatcaa caagccaaac
    30061 agcgagatac ccgcccccat tagcatcgtg ttactgatac cgtaattatt gataaatatc
    30121 ggcagtgccc aggtcgaaac aatggtgcca atacgactta aggacataat cacgcccacg
    30181 gcagaggcgc ggatatccgt cgggaagagt tcattaggat agagccactg caaattaccc
    30241 ggcccgccag agaaaaaggc atacaccgca aaggccatca ctaccagcca gatccccata
    30301 tccgggatta gccccaaaac cgccagcgcc agcgtcatca tggcaaagct gccaatcaac
    30361 aatggacgcc gtccggcagt gtttagccac agcatcggcg gaatacagcc gagcataaag
    30421 aacaggctaa tcaccacatt ccctagtgcc gcgtttttgc caacccccaa tcccaacgaa
    30481 ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag
    30541 atggcggcaa caaacagaac aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg
    30601 gtttgctgcg gctgctcttc atcgaaagcc accggttcgc caaacagttt tatcatcatc
    30661 tcttcgcact cttttactcg ccctttgcgt aataaccagc ggggagattc tggcagttcg
    30721 aatcgaccaa tcaaaatcaa caaacaaggg atcgccgcgc tacccagcat ccagcgccaa
    30781 ccaccttcca catcataaag ccagtagccg accagatcgg cgcaggtcgc gccgacatac
    30841 cacatcgcgg caataaagct gatggaaaac gcccgctgac gggtactgga gaactcggtg
    30901 atcatcgaag tggcgatggg ataatctgca ccgatgacaa tgccgatgag tacccgcatc
    30961 accaacagtt cgacggggga tgaaacacac atcgtcgcca ccgatatcac gccgatggcg
    31021 atgatatcaa tgaggaacat tttgcgccgt ccgactttat cggagatata gccaaacagc
    31081 gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg
    31141 tccagtttca gcgccggagt cagttgctcc agcgccacgc caatcattac cagtacataa
    31201 ccatccagaa acggaccacc gcttccccac agcaaaatgc ggcggtgaat agaggagaat
    31261 ttgagatcgt caaagtttct ggacggttgc atgggttatt cctgtttttt tgcaatgaga
    31321 gtcagtacgg cgttacgtat caaaccaaca tcagccgtaa cggaactcca caccaaaggt
    31381 gccgcgcggg tattcccact gttccagcgc cgaccccagc cccagaatgc gacaggtgcc
    31441 gcactccaga catccggcgt aatcgaagcg cacactgccg tcatcctgct tcttgtacag
    31501 acctgcgggg cacgctttca ccagcagctc cagcacctgt ttatcagcat cggccttcac
    31561 aacaatgtgc ggatgctctt catcgacatt gaatttattg acgcccagtt tgacgtccac
    31621 attgacggga gaagtcataa aacggtcact cctttcatgc catccttgat cagattgatg
    31681 aagcccactt tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg cgcactgccg
    31741 tcaatggtga acaggtcacg cgccacgccc accgccagtt ccgggtagcc gctaaacatg
    31801 cgtgggttat caaggaacgc cggtagtttc tggtacatgc gcatatcgcg cagcgggctg
    31861 ctctccagat gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc gcttttcatc
    31921 gctgaaagca cggtttttgc tgcagcttcc ccggcggcaa tcgccagatc cataccgcga
    31981 atggtaaaac cgaggttcat acacattccg gcggcatcac cggcaatcaa tacgccgtca
    32041 ccgaccagct ccggcagcat gttgatgcct gcttccggca ctacgtgagc ggaatattcc
    32101 accagcttgc cgcccgcgat cagcggtgca acggccggat gctgtttgaa atcttccagc
    32161 atttgcggca ccgatttttt cgcgtcatgc agatgatgca gaccacaaac cagccccagc
    32221 gacagggtat tttcattggt ataaaggaag ccgccgccca tcaggccatc ggtgggtgat
    32281 cccgcaaaca gacaagccgc gccctgatta ccctgcaact gaaaacggtc ttcgattacc
    32341 gacttcggta actcgatcag ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt
    32401 tttgccatcc ccaacttttc ggcaaggatg gagttcaccc catcagcaag gagcaccgtt
    32461 ttcgcttcaa tcacatcgcc atcggcttct acaccgacga ctttgccatc gcgctgtacg
    32521 aggttatcga cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc
    32581 aaccaggcat caaatttact gcgcaaaacg gagtaagaac gctgggatag cgaggtttcg
    32641 tcaccgttgc agtagtccat agtcatcgct gacttttccg tcataaacgc gagtttttca
    32701 tgggtgatca ggcgttctac gggggcggaa tcggcgaaac ctggaataat gtgttccaga
    32761 ctgtgggcat agagacgccc gccagtaacg ttcttggcac ccgcggaatt gccacgctcg
    32821 ataactaaca cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga accggcaagc
    32881 cctgcaccga cgatgatggc gtcaaagata tcttcggaca taagagctcc ctgtcagcgg
    32941 cagagccgta atgcagccct gccagagtgg atcaacgcgc taaagctgcg gtcagtgccg
    33001 gaaggatctt cacggcatcg ccaacaatgc cgtaatccgc gtactggaag atcggcgcat
    33061 ttttatcttt attgatggcg aaaatggttt gcgacgcgtt agcgccaacc atgtgctgga
    33121 tctgcccgga gatccccacc gccaggtaca gttcaggttt cagcatcagg ttggagatac
    33181 cgacatagcg ttcgtgctcc atccattttt cgttttctgc caccggacga gaacaggcca
    33241 actccgcacc tatcgccttg caaagctgtt ctgccagtgc aatgttctct ttgctgccaa
    33301 tgccgcgacc gacgctgacc accagacggg ctttgtcgag atcgacgctg ttgctctggc
    33361 gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc acggtgtgcg
    33421 tttcgccagt gcgtgacgcg tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca
    33481 gtaccgcata cggcgtggca atgcgttctt cgccaatcgc cagaccgccg taaaccatgt
    33541 gtttcactgt tgctttaccg tcctgtacgc tgacggtgct ggcatcgtta gacaccgcct
    33601 ctttaaggcg ataacccagt tttgccgcca gtaatttgcc gcgacgggtg tttggcaaca
    33661 gcaccagacc gtcagcgccg tgctggcgaa tagtgtcagc catgacaccg gcgtaatctt
    33721 cgatcatccg atcgtccggt ttgccgttta atttccagac atgattagcg ccgagctgga
    33781 ttgcctgtgc gccgtcggca tcattgagga caaaggcatt gatttgatta gctaaagcct
    33841 gcgcaccgtt catcagttcc ggcagacgag agggggtatc gctgaatacc cagacttgag
    33901 aaaacgtgtt catagcatcc cctgtaatta aatgactttg cgaagatttt cagcaaatgc
    33961 ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc gctgtttcgg
    34021 cgcggcaacc tgttgttcag accaggcgtt cacggcgtta aaaccaatat ccgccgccga
    34081 ccatacctgg actggctttt tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg
    34141 ggagttgata tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta aggtttcggt
    34201 ttcatcttcc agttcgcgct caacggtgag ggtatctgcc gtcagggaga taattttgct
    34261 gacgccgtta actgccggaa tattgaggat ttcgcccacc agcagaccaa cctgctgggc
    34321 ataaaggtcg gaagaaccat cgccacagag gatcagatca aagcctgctt tctgggcggc
    34381 tgcagccagt gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt catcaatcac
    34441 cacaatcagt tcatccgggc cgcgcgatag cacatcttta cgccctttgg cgttggtcag
    34501 ggctttaccg cccacactta aggctgtcac ctgcgcctct gctgcctgtt gctttagctg
    34561 gcaagccgct tcaatagcgt tgagatcgta ttggcttatt ttggcatcgg ctttgctgaa
    34621 gtctaatgaa ccatcagcat tattgaccgc aatatcctgt tcatcaggca cgcacttata
    34681 gcaagtaata atcttcattg catctccaga aatcatgaag gaaacgtcgt tcatatcggt
    34741 agtgttaatt acagatatca cgccctttaa tttggaaata aaactttaat caccaatatt
    34801 gaaaatgtca cacgcagata aatccaactt tcaatattgt taagttcctc accaatattg
    34861 aaaacatggc gtagcaaaaa gaaattttca atattgtttt atagatcacc aatattgaaa
    34921 gtcagatgct attttttacc cacaaaagaa cataacaaac tgattaataa ccaaaattaa
    34981 cgaaaaacgt cctctattca ctcgaaacaa aaatgtgaca ccaatcacag aatacagctt
    35041 attgaatacc cattatgagt tagccattaa cgcgtccacg aggttaataa taattatatt
    35101 aaatgttaac aaaaataaaa caaacgggaa cacaaaataa atattcgttt tcacagtgga
    35161 attaactcat gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc
    35221 cattaataat cgtcggcata ctttgttggc ttacagtcag agatctggat gcggcgaatg
    35281 tcgttattaa tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt
    35341 atatggtggt gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc
    35401 gtttaggtaa cgaaccgcca gaatttagca ccgccagttg gatctttatg atgttcgcct
    35461 cctgtacgtc tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca
    35521 ccccgccgtt tggcttagaa ccgaactcga caggagcgaa agagttgggg ctggcttaca
    35581 gcttgttcca ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg
    35641 cttacttctt ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc
    35701 tggtaggtga aaaacacgcc aaagggttgt tcggtaccat cgtcgacaac ttctatctcg
    35761 tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt
    35821 gtatgcaatg gttgtttgac attccgcata ccctgcaact ggacgctatc atcattacct
    35881 gctggattat cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg
    35941 ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc atcgtcagcg
    36001 gtgccagctt catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc
    36061 cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg
    36121 tgttctactg ggcatggtgg gtgatttacg ccatccagat gagtatcttc ctcgcccgca
    36181 tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat ggtgatgggg ctgacagcat
    36241 ccacctggat cctgtggacc gtgctcggta gtaacactct gctgttgatg gataaaaaca
    36301 tcatcaacat tccaaatctg atcgaacagt acggtgtggc gcgcgccatc atcgaaacct
    36361 gggccgctct gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg
    36421 ccaccgttac gctggttaac gcctgctctt ataccctggc gatgtctact tgccgcgaag
    36481 tacgcgatgg tgaagaacca ccgctgctgg tgcgtatcgg ttggtcaatt ctggttggca
    36541 ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg
    36601 ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg
    36661 cgaaacagaa ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt
    36721 ttaatttaaa tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg
    36781 aaaactggga ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg
    36841 tcaaagcact ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc
    36901 tggacgcggg gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac
    36961 caacctatgt actgtaccag ctgccgggcg ggttcaatac cttcctgcgc gaaggcacac
    37021 aagagcagat cgacaagatt atggctttcc gcggcaccgg taagcagatg tggaactcag
    37081 cgattaccga accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta
    37141 gaaatggtaa gatttatctt aatggtagta agtgttttat taccagtagc gcctacaccc
    37201 cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat
    37261 ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa acttgagaag ctcggtctgc
    37321 gtatggatag ctgctgtgaa atcacctttg acgatgtgga actggacgag aaagacatgt
    37381 tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc
    37441 tggtagccct gaccaactac ggtacggcga tgtgcgcctt tgaagatgcg gcgcgctacg
    37501 ccaaccagcg cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat
    37561 tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga
    37621 aagcagacaa cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca
    37681 atgcggcatt tgaagtggtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg
    37741 gcaaccaccg catcagccgc ttctggcgtg acctgcgtgt agaccgcgtt tccggaggat
    37801 ctgacgaaat gcagatcctg acgctgggtc gtgcggtgct gaagcaatac cgctaagtcc
    37861 gtgcgctgcc tgatgcgacg ctgacgcgtc ttatcaggcc tacgggagca cgaatgtaag
    37921 ccggataagg cgtttacgcc gcatccggca gtcatgcgct acataaattt ttcaggagct
    37981 aataatggat catctaccca tgccgaaatt cggaccgctg gccggattgc gcgttgtctt
    38041 ctccggtatc gaaatcgccg gaccgtttgc cggacaaatg ttcgcagaat ggggcgcgga
    38101 agttatttgg atcgagaacg tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc
    38161 gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg
    38221 cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gtaaaggtcc
    38281 ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt
    38341 tatcgctcac ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc
    38401 ctataacacc atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca
    38461 gccaatgcct gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc
    38521 ggcgctggca gcactgcata aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc
    38581 catgtatgaa gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg
    38641 cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta
    38701 taaatgtgcc gacggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg
    38761 ctttaaagat attggcctcg cacatctact cggtacgccg gaaatcccgg aaggcactca
    38821 gcttatccac cgtatcgaat gcccttacgg cccactggta gaagaaaaac tcgatgcctg
    38881 gctggcggca cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg
    38941 tgccaaagta ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc
    39001 aatcactcag tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa
    39061 attcaaaaat aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc
    39121 tgccattttg aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaaagg
    39181 tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg cttgcctgac tagcgcccgc
    39241 ccaataacaa acgaacaaaa atggatagag gtgcaatgga tatcattggc ggacaacatc
    39301 tacgtcaaat gtgggacgat ctggcggacg tttacggtca taaaacggcg ctgatttgtg
    39361 aatccagcgg cggagtcgtt aaccggtata gttatcttga gttaaatcag gagattaacc
    39421 gcacggcaaa cctgttttat acgctgggga ttcgcaaagg caacaaggtt gcactacatc
    39481 tcgacaactg cccggaattt atcttttgct ggttcgggct ggcaaaaatt ggcgcgatta
    39541 tggtgccgat taacgcccgc ctgttacgcg aggaaagcga gtggatcttg caaaatagcc
    39601 aggcgtgcct gctggtgacc agtgcgcaat tttatcctat gtatcaacag attcagcagg
    39661 aagatgccac tcaattgcgg cacatttgcc tgacagatgt ggcacttccc gctgatgatg
    39721 gagtgagttc atttactcaa ctgaaaaatc aacaacctgc caccttgtgc tatgcaccgc
    39781 cgctatcgac tgacgatacg gcggaaattc tcttcacctc cggcaccacc tcccgaccga
    39841 aaggtgtggt gattacccat tacaacctgc gcttcgctgg atattactcc gcctggcagt
    39901 gtgcactgcg tgacgatgac gtctacatga cggtaatgcc tgcgtttcat atcgattgcc
    39961 agtgtactgc ggcgatggcg gcgttttctg ccggggccac ctttgtgctg gtcgagaaat
    40021 acagcgcccg cgccttctgg ggacaggcgc agaagtaccg cgccaccatt accgaatgta
    40081 ttccgatgat gatccgtacg ttgatggtgc agccgccttc agcgaacgat cggcaacacc
    40141 gcctgcggga agtgatgttt tatctcaact tgtcggagca ggaaaaagac gcattttgtg
    40201 aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac ggaaaccatt gtgggcatta
    40261 tcggcgatcg ccctagcgat aaacgacgct ggccgtcgat tggtcgggcg gggttttgct
    40321 acgaagcgga gatccgcgac gatcacaatc gcccgctccc ggcaggtgag atcggtgaaa
    40381 tctgtattaa aggcgtacca gggaaaacca tcttcaaaga gtattttctc aacccgaaag
    40441 ccactgcgaa agtgctggaa gccgacggct ggctacatac cggcgatacc ggataccgcg
    40501 acgaagaagg ctttttttat ttcgtcgatc gccgctgcaa tatgatcaaa cgcggtggcg
    40561 agaatgtctc ctgcgtggag ctggaaaata tcatcgccac ccatccgaaa attcaggaca
    40621 tcgtggttgt gggtattaaa gattcgattc gcgatgaagc catcaaagca tttgtggtgc
    40681 tgaatgaagg tgaaacattg agcgaagagg aattttttcg cttctgcgaa caaaatatgg
    40741 cgaaatttaa agtgccctct tatctggaga tcagaaaaga tctgccacgt aattgctcgg
    40801 ggaaaataat tagaaagaat ctgaaataaa tgaaacgtca gggaactacc ctgccagcta
    40861 ataatcacgc cctaaaacaa tatgcatttt ttgcagggat gttgtcgtcc ctaaaaaagc
    40921 aaaaatggag aaaaggaatg agtgaatcat tacatctgac ccgcaatgga tcaattctgg
    40981 aaattaccct tgatcgccca aaagcgaatg ctattgatgc aaaaaccagc tttgaaatgg
    41041 gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt attaccggtg
    41101 ccggagagaa attcttttcc gcgggctggg atttaaaagc ggcagcagaa ggcgaagcac
    41161 cggatgctga ctttggcccg ggtggttttg cgggattaac cgaaattttc aatctcgaca
    41221 aaccagttat cgcagctgtg aacggctacg cctttggcgg cggtttcgaa ctggcgctgg
    41281 cggcagattt tattgtttgt gccgataacg ccagcttcgc cctgccggaa gccaaactgg
    41341 gcatcgttcc tgacagcggc ggtgtgctgc gtctgccgaa gatcctgccg cctaccatcg
    41401 tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga agaggcgctg cgttggggga
    41461 tagtcaaccg cgtggttagc caggcggaac tgatggataa cgcccgcgaa ctggctcagc
    41521 agctggttaa cagcgccccg ctggcgattg cggcgctgaa agagatctac cgcaccacca
    41581 gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg aaacactatc
    41641 catcggttct gcattcggaa gatgccattg aagggccgct ggcgtttgcc gagaagcgcg
    41701 atccggtgtg gaaaggacgt taacaaccgt gagctattac gcctttgagg ggttaattcc
    41761 ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc ttgattggcg atgtgattgt
    41821 gggagccggt gtctacatcg gcccactcgc ctcactgcgt ggtgactacg ggcggttgat
    41881 cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg catggctact gcgacactga
    41941 caccatcgtt ggggaaaacg gccatatcgg gcatggagcg atcctgcatg gttgtgtgat
    42001 tggtcgcgat gcattggtcg ggatgaacag cgtaattatg gatggcgcgg tcattggcga
    42061 agagagcatt gttgccgcaa tgagctttgt caaagcaggc tttagtggcg agaaacgcca
    42121 gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt agtgacgacg agttacactg
    42181 gaaacggttg aataccaaag agtatcagga tcttgtcggg cgctgtcatg catcgttaca
    42241 tgaaacgcag ccactgaggc aaatggagga aaatcgcccc cgtttgcagg ggacgacgga
    42301 tgtgacgcct aaacggtgat cttgccggat gcgacctggt tgctggataa gatgctacaa
    42361 catcgtatct ggcaagcagg acgcccggta tcaacgagat aaaattaacg acgcatactc
    42421 tttgacaaca tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga
    42481 atacgcggtg tctttcttac gccgactaat ttttcccgac tgttattgcg caggcgcgcg
    42541 taaatttgct catcgatatc gaccacttta accagtcgct gacactggca tccccgccct
    42601 tccagcttat tagggatcat cttaacttcg cagcttattt ctgtgacttc cgacagaata
    42661 taagtcaggg tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt
    42721 gctatcaccc accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt
    42781 tcaacatatc cttcacacat acgaactcca gtttcacaaa ttaaaagtct ggctatgact
    42841 cgttttgaag caattaaaca agaccatatt aaaattgtgg atatatcgat agtatgcaac
    42901 ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca
    42961 aaagatttac ttaatgggca aaaaaaaacg gtcctcatca gagaaccgta ttagttattc
    43021 gatcaaatta gcggatgaaa aatatccgcc acgacacgct attatttgat ctgtgcgtgc
    43081 atttcctgca ccgaaattac tttttcagtc gcatcggcat tcagcgccat cgcggtagcg
    43141 aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc
    43201 acgcgggagt cttcaatcgc acgacggcct gaggtggtgt tgatgatgta ggtatattcg
    43261 ccattcttga tacggtcctg aatgtgcgga cggccttcat gcaccttgtt taccagacgc
    43321 ggattgatac ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag
    43381 ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg
    43441 gaaagcagcg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggcg
    43501 aacgcttcag cgaaggtgcg gcccacgccc atgacttccc cggtagagcg catttctggc
    43561 cctaacagcg ggtcaacgcc tgggaattta ttgaacggca gcaccacttc tttcaccgag
    43621 tagtacggcg gaataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc
    43681 acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacagta
    43741 cgcgccgcac gcgggtttac ttcaatcagg taaacttcgt tgtttttcac cgcaaactgc
    43801 acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc
    43861 acatcctgaa tttcctgact taaggtgtac gctggcagcg aacatgcgga gtcaccggag
    43921 tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg
    43981 cagatggcat ccacgtcaac ttctactgcg tcatcaagga aatgatccag caacactggc
    44041 gcatcgttag acacgctaac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag
    44101 acgatttcca tcgcccgacc gccgagaacg taagacggac gcaccaccag cgggtagcca
    44161 atctcttttg ccttctcaac tgccatttca atagcagtaa cggtggcgtt cgccggttgt
    44221 ttcagtttca gacggtcaac cgcatgctgg aagcgttcac ggtcttccgc acggtcgata
    44281 gcatccgggc tggtgccgat aaccggtacg ccagcggctt ccagtgcacg cgccagtttc
    44341 agcggggttt gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt
    44401 tccagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg
    44461 gtagagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcacgcagc
    44521 gccagcgagg cgtgtacgca gcagtagtcg aattcgatac cctgaccgat acggtttgga
    44581 ccgccgccaa gcaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct
    44641 tcataagtgg agtacatgta agcggtgtcg gtggcaaact ctgccgcaca ggtatccacg
    44701 cgcttgtaga ccgggtgcag gtcatattgg tcacgcagct tacggatttc cgcttcgcgt
    44761 acgcccgcca gttttgccaa gcgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg
    44821 aattcagcgt tcaggccagt gatgcccact tccgccactt tctcttccag acgcaccagt
    44881 tcttcaatct gtaccaggaa ccagcggtca atgttagtca ggttgaagac gccgtccaca
    44941 gacaggcccg cgcggaaagc atcggcgatg taccagatac gctctgcgcc tgcgtctttc
    45001 agttcgcgac ggattttggt taacgcttcc gggtcatcca ggctcacttt cgggtcgaag
    45061 ccagttgcgc ccacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga
    45121 ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcaccg
    45181 gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg
    45241 aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtctag ggtgtaaccc
    45301 accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc
    45361 gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc
    45421 accgcaaact ggacattgga accaccggtt tcaacgccga tttcacgcag caccgccatc
    45481 gaggcgttac gcatgatttg atattctttg tcagtcagcg tttgggctgg cgcgacagtg
    45541 atggagtcac cggtgtggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg
    45601 cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac
    45661 tcatcaatca gcaactcttt ggtcggagag agatccagac cgcgggcgca aatttcttca
    45721 aactcttcac ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga
    45781 ataatgcacg ggaagcccac gtcagcggca acagccagcg cttcttccat cgtgtgtgcg
    45841 ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg
    45901 tcttctgctt tatcaatcgc atcggcagtg gcaccaatca tggtgacacc gaactcttcc
    45961 aacacgccct gacgttccag ctccagcgcg cagttcagcg ccgtctgacc gcccatggtt
    46021 ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtaccacttc ccagtgaatc
    46081 ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag
    46141 ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag
    46201 tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt
    46261 tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt
    46321 actgctcaat taacttgata aagtggtcga acaacggcgc ggcgtcgtgt ggaccagggc
    46381 tggcttcagg gtgaccctgg aagctgaacg ccggtttatc gatacgatga atgccctgta
    46441 acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat
    46501 ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa
    46561 ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca
    46621 gcgccagcag ctgatgaccg agacagatgc cgaataccgg aatatcggtt tcgaggaact
    46681 tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga
    46741 agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg
    46801 tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat
    46861 aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca
    46921 acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat
    46981 ccatgccgtt cagacctggg aacgcgcggg ctttttctaa cgccagcgcc gcatccgggt
    47041 tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct
    47101 tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt
    47161 cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg
    47221 catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat
    47281 aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg
    47341 tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac
    47401 cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa acacctccat
    47461 agaatattca ctcactttat ttgcatatta attcacagtg atgacgtaaa tcaatgcaaa
    47521 tctgcttacc gacgaatttc tggcaaacgg cggcattctg gagatatggg cgctaaaagt
    47581 caacttaatg gcaggatttt ttatattatt ttgtggctca tgcatgtttt acaggcatta
    47641 agcgacaaaa acatcatcta acctgttctg gaaaacgctt gcgcagcaag aagattgcat
    47701 gaaataaaaa cgagcaggaa aaagtggacc aaatggtcaa aatttacatt aaaacaacaa
    47761 aaatagaaga cgaaacatgt aatttttggc agttatagaa aatttaaaag cataacacca
    47821 aaaataaaag ggctaaaaat taaagccctt tataattaat aaattaaatg tgttactttt
    47881 gcatcataat aaacattttg tggttacaaa ttattgagat caagcacatc tcgcatatca
    47941 aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcacc gttagcaaat
    48001 gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
    48061 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtaccaggc
    48121 acacgttcac cggtatggcc ttcacgactg tagaccgcgc aatctttcag atctttatca
    48181 agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
    48241 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct
    48301 ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc
    48361 gcaatatcgg cggcggcgtc acgaattgcc tgtttaccgg cttcgtcaaa ccccgtagtg
    48421 ccgatcacca tccctttgcc atgctgccga caaaaagcga gatggttcag cgtaccttcc
    48481 ggacgggtaa aatcgataaa cacatcaaaa tcatctttta tcgcatcaag actgctttgc
    48541 acggtaacgc ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa
    48601 gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag tgccgcctga
    48661 atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca
    48721 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
    48781 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
    48841 agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag gttacggcac
    48901 cagagccagc acctcagcca cccactgttg gaagcctttt acattcagat ccaatgccac
    48961 ctgtacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt
    49021 aaattcgcct tgagtttcta ccgcgacaaa acacggcttg agggtgaata agtccgggcg
    49081 caccagccag gcgatggcgc agagatcgtg cattcgcaga ccgctttgca tactgccgct
    49141 acggtagtgg ctaaacaggg cgtggagcat ttttccggtg cgattcaact ccggtaaagt
    49201 agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc acatgacaat
    49261 ttcgatgccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc
    49321 ggcatttggt gtacagttgc cgcgaccagc agaaccgccc atgatcacca gacgacgaat
    49381 atacggttta cattccgggc attgtaaaag taacagcgca atattggtta acgggccgat
    49441 ggccaccagg gtaacgggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc
    49501 cggtatcccg agtggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc
    49561 gccgtgcaca gatgctgcat cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc
    49621 gagcggaatc tccgcattcc agaaatgcag cagttgcagg gcattgcggg tagttttctc
    49681 aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gggcaaaaat
    49741 cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa
    49801 acgcatgttt tcttcataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg
    49861 ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa
    49921 acaatatttt cttcacggcc ttccagcgga atggcttcac caccacccaa ctgctgcaaa
    49981 cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg
    50041 catttaacct ctttcaccca ctcttcctgg atatctttcg catcgtcaat caaaaacgcg
    50101 tgtttgccca tacgctgggc cagctccgcc agacggttgg agttggagga gtttttcgaa
    50161 ccgaccacca acacaacttc cgcctgttct gccagggcgc gtaccgcttc ctgacggtta
    50221 gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc
    50281 agcgcgtcga tcacatcaga cgtatcatcc accgacagcg tggtttgggt cataaaggag
    50341 agcttctctt cgtttttgac cgtcagtttc cacacatcgt ctggcgattc gaccagatac
    50401 attccccctt cggggttgct gtactgcccc atcgtccctt ccacttccgg gtgcccggcg
    50461 tggccgatga gaatagattc ttcgccacgg cgactggcgc gggcaacttc catatgcact
    50521 ttggtcacca gcggacaggt ggcatcaaac accgtcaaat cgcggctttt cgcttcgtta
    50581 cgaaccgcct gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggcacttcg
    50641 ctaatctgct caataaagat agccccacgc tcgcgcaagc tatcgaccac gtagcggtta
    50701 tgcaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca
    50761 atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc
    50821 atgttacgcc tccagtgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg
    50881 cccggccagc ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac
    50941 accaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc
    51001 gcctgcatcc ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc
    51061 aaacgccgca tctggctcca gcgagaaggt ggttttgtcg cccactttca gccccagcag
    51121 gtgttgctcc agcccttcag aaagagaagc atcgcccagg cggaacagcg ccggtttacc
    51181 gttgttgcga gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag
    51241 gacggcgcta ttgctctgta cagattcaga catgaaggtt tgctcttatt tttgaggatt
    51301 tttgccggat gcggcctacg atttcgtagc aatctgtagg ccgcatccgg cagggtttat
    51361 tattgttttt tcgctttgga aggtaaaaac ccttccagca cgatcagtgc cgcaccgaca
    51421 caaatggcag tatcggcaag gttgaaggtg gcgaagtgcc agtcgccgac gtagaagtcg
    51481 atcatatcga caacgaagcc gtgccacagg cggtcgaaca ggttgcccag cgcgccgcca
    51541 ataatcagcg cgtaagcgat attgtttagc ttctgcgtgg ccttcgagcg atacatcatc
    51601 actgccagga gcacgctaat accaatcgca ataccggcaa agaaccaacg ctgccagccg
    51661 ccgctatcgg caaggaaact aaacgccgcg ccatagttac gcgcataatg cagattaagc
    51721 gacgggaaca gcgggaccgt atcccccaga gcaaagttct ggaggatcag gtatttgctg
    51781 cccagatcga taatcagcac gactaccacc agccacagcc agcgtagccc tgttgaacag
    51841 atcgattgac tcatcaggca aacttacgtt tttcaccgtc accggcgacg ttgctgacac
    51901 agcggccgca gatttctgcg tgttccgcca ccttgccgac atcctgggtg tagtgccagc
    51961 agcgtgggca cttctcacct tcggctttac tcaacgcgac tttcagccct ttgagtactt
    52021 cgctctgctg agcatcagca ggtgcgtcgt tatagtctgc aacggtagcg ccggaggtca
    52081 acaggacaaa tcgtaattca tcgcccagcg cggtcagttt cgccgccagt tccggttctg
    52141 catacaaggt tactgccgct tccagcgagc cacccacttt cttgtcggca cgcgcttgct
    52201 caatgacttt gttcacttcg ccacgcactt tcaacagctc gtcccagaac gcatcgttca
    52261 ttgcttcact gtctgccaga ccaaacaggc cttcgtacca ctcaccggtg aagacgtatt
    52321 tttcacgttc gcccggcaga tagccccaca cttcatcagc ggtgaaggag aggattggtg
    52381 ccatccagcg caccagcgct tcagcgatgt gatacagcgc agtctggcag ctacgacgcg
    52441 ccacgctatc cgctttggcg gtgtactgac ggtctttgat gatgtcgagg tagaaggaac
    52501 ccatctcaac ggagcagaag cgcatcagac gctgtaccac ttcgtggaaa tcgtatgctt
    52561 cgtacgcctt gaggatgtct tcctgtgccg ctttcgcaca acctacggcc cagcgatcca
    52621 gtaccaccat ctcttccggt ttcaccatat cttttgctgg atcaaaacca ttcaggtttg
    52681 ccagcaggaa gcgcgcggtg ttacggatac gacgatagct atcggcagca cgtttcagga
    52741 tctcgtcaga aacggccatt tcaccggtgt agtcggttga tgccacccac agacgcagaa
    52801 tatccgcgcc cagtttgttc atcacatcct gcggcgaaac ggtgttgccg atggatttag
    52861 acatcttgcg accctgacca tccacggtaa agccgtgggt cagtacctga cgatacggcg
    52921 ctttgccttt catcgcggtg gagatcatta gggaagacat gaaccaacca cggtgctggt
    52981 cagaaccttc cagatacatg tccgctgcgt gaccggcaaa ttccggacgc acgtcaacaa
    53041 cagaagagtg ggtagatccg gagtcaaacc atacgtcgag agtatccggc actttcacat
    53101 actggtcagc ttcgtcgccg aggatctctt tcgcatcgag atcccaccac gcctggatgc
    53161 cgtcaacttc aacgcgttta gccacttctt ccatcagttc gagggtacgc ggatgcagct
    53221 cttccgtgtc tttgtgcacg aacagtgaca tcggtacgcc ccaggtgcgc tgacgggaga
    53281 tacaccagtc aggacggtta gcaaccatcg actcgatacg cgcctggccc cagtccggga
    53341 tccactgcac gcctttgatc tctttcagtg actgcgcacg cagacctttc tgatccatgc
    53401 tgacgaacca ctgcggtgtc gcgcggaaga tgatcggcgt tttgtgacgc cagcagcacg
    53461 gatagctgtg ctgcattttc tcaacgtgca gcagcgcgcc tttttcctgc agcagcgcaa
    53521 cgacgatgtc gttcgctttg aagacgttca cgccatccag cgtcggataa gtgcccggca
    53581 gataagtgcc gtccgggcca accgggttag cggtttccag gccgtatttc tgaccgatca
    53641 catagtcgtc cgggccgtgg ccaggcgcgg tgtgaacggc accggtaccg gcatccaggg
    53701 taacgtgatc gccgaggatt gccggaacgt cgaagcccat aaacggatgg gtaaagcgca
    53761 gcagctcaag ctctgcacct tttaccgtgc cgagaatggt gtaatcggtc acgccgatac
    53821 gctgcattac gctttcaacc agatctttcg ccagaatcgc ggcctgaccg tcgatctgca
    53881 ccagcgcata gtcgaaatca ggtgcaatag agattgcgcg gttcgcaggc aaagtccacg
    53941 gcgtagtggt ccagatcacc agcgagattg ggccgttaac gttgcttacg ccaaatttcg
    54001 ctttcagcgc atcctgatcg accgcctgga aagcgacgtc aatggacgga gaagttttgt
    54061 cgtaatactc aacttccgct tctgccagtg cagaacggca gtctacgcac cagtgcaccg
    54121 gcttcgcgcc tttgtgcagg tgaccattgc cgatgatttt gcccagcgcg cggatgatgt
    54181 tggcttcagt tttgaagtcc atagtcagat acgggtgcga ccagtcgccc agcacgccca
    54241 gacggataaa gtctttgcgt tgaccgtcaa cctgggtcgc cgcgtattcg cggcacttgg
    54301 cgcggaactc ggcggcggtg aatttctcac ccggcttacc gtattcttgc tctactttca
    54361 gctcgatcgg cagaccgtgg cagtcccagc caggcacata cggcgagtca taaccggaaa
    54421 gccctttgga cttcacgata atgtctttca gaatcttgtt aaccgagtga ccaatatgaa
    54481 tgctgccatt cgcataagga gggccatcat gcagaatgaa ggtttttttg ccttttttag
    54541 ccgcacggat gatgccgtac agatcatcat cagtccaacg cgccagcatt cccggttcgc
    54601 gcttggcgag atcgccacgc atcgggaacc ctgtttccgg caaattcagg gttgatttat
    54661 agtcactcat cagattctcg gttccgtgtt tcggtttgat tacataacag gcttaagccg
    54721 gttttgttag cccaaaaaat tcgcgggcgg ttaattcatc acgcgcaatc tgcgctttca
    54781 gttcgtccag cgacgcaaat cgctgctcat tgcgtatttt tttacgcagc actacttgta
    54841 tatggcgacc gtaaaggtcc attgcaacat ctaacaaatg cacttccagc tgctggcgaa
    54901 taccggcaac cgttgggcgt gttccgatgt ttgccacgcc gggtaacggc ttttcaccga
    54961 ggcccagcac ttctaccgca taaacccctt tcaccgggga aacctgacgg cgtagcggta
    55021 cattcgccgt cgggaaacct atagtgcgcc ctaattcatc accgtggact acacgcccgg
    55081 agatggcaaa cgggtgcccc agtaaacttt ctgccagagc cagattgtca tcagccagcg
    55141 cctgacgcac ggcggtgctg ctgatacgca caccaccttc gcaaaaagtt tgcgtactgg
    55201 tgatatcgaa gccgtattcc atgccagctt tctgtaataa caagaaatcg ccttcacgac
    55261 cagcgccaaa gcggaaatca tcacctacgg caagaaattt tacgcgcaaa tgcttcacca
    55321 aaagatcgct gatgaaattt tgcgcggtta acgccgcaaa acgcctgtcg aaacgcacgc
    55381 acagcacgta atcaacgcca cactctgcaa ggtaacgcag tttttcccgc agccgggtca
    55441 gtcttgccgg ggctttatcg gtagcaaaca gttccagtgg ttgaggttca aaaagcatca
    55501 ccatcaccgg taagttgcgc ttgcgccctt cttcctgcaa gccctgtaac agcgcgcgat
    55561 gaccgcgatg cacgccgtcg aaattaccaa tagtcagcac acacccttct tgcggggcct
    55621 ggctgagatt atgtatgccg cgtatcagct tcatgtctgg ctcaaaacag tgaaaatcgt
    55681 ccgagtatac cttgtacagc ggtcaaggtt aaccggcgat tgagtaccga aatcagaaag
    55741 aagtggcttt tcattgccat ggcgcaaatc acgggaagaa actgaccgcc tgctgcaatt
    55801 tttatcgcgg aaaagctgta ttcacacccc gcaagctggt agaatcctgc gccatcacta
    55861 cgtaacgagt gccggcacat taacggcgct tatttgcaca aatccattga caaaagaagg
    55921 ctaaaagggc atattcctcg gcctttgaat tgtccatata gaacacattt gggagttgga
    55981 ccttggctaa tatcaaatca gctaagaagc gcgccattca gtctgaaaag gctcgtaagc
    56041 acaacgcaag ccgtcgctct atgatgcgta ctttcatcaa gaaagtatac gcagctatcg
    56101 aagctggcga caaagctgct gcacagaaag catttaacga aatgcaaccg atcgtggacc
    56161 gtcaggctgc taaaggtctg atccacaaaa acaaagctgc acgtcataag gctaacctga
    56221 ctgcacagat caacaaactg gcttaatcgc caatttgctg aagctttgtg aaaaagcccg
    56281 cgcaagcggg tttttttatg cctgctgctt ttgtgatgcg tgagagaaca cactgccggt
    56341 tgccagatag tcaaaagaga catgccgttg tactatctga actcttcgtg gcttaagaat
    56401 cgtagaacca tcggctgata cattctgaac aagaatatct actcaatctc cagactcccc
    56461 cacaaagaat atggatattg tgatacacat tgaggtatta aacttaagac cactctattt
    56521 ttgaattaaa ttcctatata ctttaaccat tcataaatat ctcctcatta gac
//
LOCUS       DAJTXY010000013        55391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTXY010000013 DAJTXY010000000
VERSION     DAJTXY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.013"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..356
                     /locus_tag="OO566_000885"
     CDS             <1..356
                     /locus_tag="OO566_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6604273.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="OO566_000886"
     CDS             complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="OO566_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6604274.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="OO566_000887"
     CDS             complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="OO566_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6604275.1"
                     /translation="MKHLHQFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3111..3263
                     /gene="mokC"
                     /locus_tag="OO566_000888"
     CDS             3111..3263
                     /gene="mokC"
                     /locus_tag="OO566_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6604276.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="OO566_000889"
     CDS             complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="OO566_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6604277.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="OO566_000890"
     CDS             complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="OO566_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6604278.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6879..7283
                     /locus_tag="OO566_000891"
     CDS             6879..7283
                     /locus_tag="OO566_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6604279.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7309..8022
                     /gene="msyB"
                     /locus_tag="OO566_000892"
     CDS             7309..8022
                     /gene="msyB"
                     /locus_tag="OO566_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6604280.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8171..8737
                     /gene="satP"
                     /locus_tag="OO566_000893"
     CDS             8171..8737
                     /gene="satP"
                     /locus_tag="OO566_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6604281.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8772..9359)
                     /gene="mog"
                     /locus_tag="OO566_000894"
     CDS             complement(8772..9359)
                     /gene="mog"
                     /locus_tag="OO566_000894"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6604282.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9474..10427)
                     /gene="tal"
                     /locus_tag="OO566_000895"
     CDS             complement(9474..10427)
                     /gene="tal"
                     /locus_tag="OO566_000895"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6604283.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10706..12160
                     /locus_tag="OO566_000896"
                     /pseudo
     CDS             10706..12160
                     /locus_tag="OO566_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12230..13006
                     /gene="yaaA"
                     /locus_tag="OO566_000897"
     CDS             12230..13006
                     /gene="yaaA"
                     /locus_tag="OO566_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6604284.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13159..13455)
                     /locus_tag="OO566_000898"
     CDS             complement(13159..13455)
                     /locus_tag="OO566_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6604285.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13669..14955)
                     /gene="thrC"
                     /locus_tag="OO566_000899"
     CDS             complement(13669..14955)
                     /gene="thrC"
                     /locus_tag="OO566_000899"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6604286.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14956..15888)
                     /gene="thrB"
                     /locus_tag="OO566_000900"
     CDS             complement(14956..15888)
                     /gene="thrB"
                     /locus_tag="OO566_000900"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6604287.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15890..18352)
                     /gene="thrA"
                     /locus_tag="OO566_000901"
     CDS             complement(15890..18352)
                     /gene="thrA"
                     /locus_tag="OO566_000901"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6604288.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18378..18497)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18433..18498)
                     /gene="thrL"
                     /locus_tag="OO566_000902"
     CDS             complement(18433..18498)
                     /gene="thrL"
                     /locus_tag="OO566_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6604289.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18712..19398)
                     /locus_tag="OO566_000903"
     CDS             complement(18712..19398)
                     /locus_tag="OO566_000903"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6604290.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19798..19938)
                     /locus_tag="OO566_000904"
     CDS             complement(19798..19938)
                     /locus_tag="OO566_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604291.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20034..20750
                     /gene="arcA"
                     /locus_tag="OO566_000905"
     CDS             20034..20750
                     /gene="arcA"
                     /locus_tag="OO566_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6604292.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20809..22161)
                     /gene="creD"
                     /locus_tag="OO566_000906"
     CDS             complement(20809..22161)
                     /gene="creD"
                     /locus_tag="OO566_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6604293.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22219..23643)
                     /gene="creC"
                     /locus_tag="OO566_000907"
     CDS             complement(22219..23643)
                     /gene="creC"
                     /locus_tag="OO566_000907"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6604294.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     VAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(23643..24332)
                     /gene="creB"
                     /locus_tag="OO566_000908"
     CDS             complement(23643..24332)
                     /gene="creB"
                     /locus_tag="OO566_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6604295.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSLGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24345..24818)
                     /gene="creA"
                     /locus_tag="OO566_000909"
     CDS             complement(24345..24818)
                     /gene="creA"
                     /locus_tag="OO566_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6604296.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25029..25898
                     /gene="robA"
                     /locus_tag="OO566_000910"
     CDS             25029..25898
                     /gene="robA"
                     /locus_tag="OO566_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6604297.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25895..26542)
                     /gene="gpmB"
                     /locus_tag="OO566_000911"
     CDS             complement(25895..26542)
                     /gene="gpmB"
                     /locus_tag="OO566_000911"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6604298.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26594..27115
                     /gene="yjjX"
                     /locus_tag="OO566_000912"
     CDS             26594..27115
                     /gene="yjjX"
                     /locus_tag="OO566_000912"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR6604299.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(27200..27526)
                     /gene="trpR"
                     /locus_tag="OO566_000913"
     CDS             complement(27200..27526)
                     /gene="trpR"
                     /locus_tag="OO566_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6604300.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27616..29553)
                     /gene="sltY"
                     /locus_tag="OO566_000914"
     CDS             complement(27616..29553)
                     /gene="sltY"
                     /locus_tag="OO566_000914"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6604301.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     EIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29764..31431
                     /gene="ettA"
                     /locus_tag="OO566_000915"
     CDS             29764..31431
                     /gene="ettA"
                     /locus_tag="OO566_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6604302.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31538..31705)
                     /locus_tag="OO566_000916"
     CDS             complement(31538..31705)
                     /locus_tag="OO566_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604303.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31739..32971)
                     /gene="nadR"
                     /locus_tag="OO566_000917"
     CDS             complement(31739..32971)
                     /gene="nadR"
                     /locus_tag="OO566_000917"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6604304.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32992..34374)
                     /gene="radA"
                     /locus_tag="OO566_000918"
     CDS             complement(32992..34374)
                     /gene="radA"
                     /locus_tag="OO566_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6604305.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34423..35391)
                     /gene="serB"
                     /locus_tag="OO566_000919"
     CDS             complement(34423..35391)
                     /gene="serB"
                     /locus_tag="OO566_000919"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6604306.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35497..36141
                     /locus_tag="OO566_000920"
     CDS             35497..36141
                     /locus_tag="OO566_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6604307.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36169..37185
                     /gene="lplA"
                     /locus_tag="OO566_000921"
                     /pseudo
     CDS             36169..37185
                     /gene="lplA"
                     /locus_tag="OO566_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37217..37480
                     /locus_tag="OO566_000922"
     CDS             37217..37480
                     /locus_tag="OO566_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6604308.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37641..38360)
                     /gene="deoD"
                     /locus_tag="OO566_000923"
     CDS             complement(37641..38360)
                     /gene="deoD"
                     /locus_tag="OO566_000923"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6604309.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38417..39640)
                     /gene="deoB"
                     /locus_tag="OO566_000924"
     CDS             complement(38417..39640)
                     /gene="deoB"
                     /locus_tag="OO566_000924"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6604310.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39692..41014)
                     /gene="deoA"
                     /locus_tag="OO566_000925"
     CDS             complement(39692..41014)
                     /gene="deoA"
                     /locus_tag="OO566_000925"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6604311.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLDGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41141..41920)
                     /gene="deoC"
                     /locus_tag="OO566_000926"
     CDS             complement(41141..41920)
                     /gene="deoC"
                     /locus_tag="OO566_000926"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6604312.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42178..43719
                     /locus_tag="OO566_000927"
     CDS             42178..43719
                     /locus_tag="OO566_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6604313.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDTRCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43691..44554
                     /locus_tag="OO566_000928"
     CDS             43691..44554
                     /locus_tag="OO566_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6604314.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44768..45550)
                     /locus_tag="OO566_000929"
     CDS             complement(44768..45550)
                     /locus_tag="OO566_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6604315.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45547..46620)
                     /locus_tag="OO566_000930"
     CDS             complement(45547..46620)
                     /locus_tag="OO566_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6604316.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46742..46903)
                     /locus_tag="OO566_000931"
     CDS             complement(46742..46903)
                     /locus_tag="OO566_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6604317.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(47030..47635)
                     /gene="osmY"
                     /locus_tag="OO566_000932"
     CDS             complement(47030..47635)
                     /gene="osmY"
                     /locus_tag="OO566_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6604318.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(48028..49617)
                     /gene="prfC"
                     /locus_tag="OO566_000933"
     CDS             complement(48028..49617)
                     /gene="prfC"
                     /locus_tag="OO566_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6604319.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49708..50385)
                     /gene="yjjG"
                     /locus_tag="OO566_000934"
     CDS             complement(49708..50385)
                     /gene="yjjG"
                     /locus_tag="OO566_000934"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6604320.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50400..50846)
                     /gene="rimI"
                     /locus_tag="OO566_000935"
     CDS             complement(50400..50846)
                     /gene="rimI"
                     /locus_tag="OO566_000935"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6604321.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50815..51228)
                     /gene="holD"
                     /locus_tag="OO566_000936"
     CDS             complement(50815..51228)
                     /gene="holD"
                     /locus_tag="OO566_000936"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6604322.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51331..52362
                     /gene="rsmC"
                     /locus_tag="OO566_000937"
     CDS             51331..52362
                     /gene="rsmC"
                     /locus_tag="OO566_000937"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6604323.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVITQTGRFKVYRA
                     IMTRQAKKG"
     gene            52639..52725
                     /locus_tag="OO566_000938"
     tRNA            52639..52725
                     /locus_tag="OO566_000938"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52673..52675,aa:Leu,seq:cag)
     gene            52754..52840
                     /locus_tag="OO566_000939"
     tRNA            52754..52840
                     /locus_tag="OO566_000939"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52788..52790,aa:Leu,seq:cag)
     gene            52875..52961
                     /locus_tag="OO566_000940"
     tRNA            52875..52961
                     /locus_tag="OO566_000940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52909..52911,aa:Leu,seq:cag)
     gene            complement(53000..53236)
                     /locus_tag="OO566_000941"
     CDS             complement(53000..53236)
                     /locus_tag="OO566_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6604324.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53377..54165
                     /gene="fhuF"
                     /locus_tag="OO566_000942"
     CDS             53377..54165
                     /gene="fhuF"
                     /locus_tag="OO566_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6604325.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(54203..54880)
                     /gene="bglJ"
                     /locus_tag="OO566_000943"
     CDS             complement(54203..54880)
                     /gene="bglJ"
                     /locus_tag="OO566_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6604326.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQEFFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(54838..>55391)
                     /locus_tag="OO566_000944"
     CDS             complement(54838..>55391)
                     /locus_tag="OO566_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6604327.1"
                     /translation="LAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        13258 a        14946 c        13830 g        13357 t
ORIGIN      
        1 attatcagtt tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt
       61 ttgaacccag tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca
      121 gcatattaaa aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac
      181 tctccaatga tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc
      241 atccccatga aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca
      301 atgtctcaag cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta
      361 tctcccccac cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc
      421 agaataatcc gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa
      481 aatagcatgg tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc
      541 ggcataaaaa tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc
      601 aaaagctttc atcaaagcgg catcatcaaa ctcgccgagg atttctacgt ttaatccctg
      661 ggagttaaac cagttaagca atttgcgccc taacattgaa cgtcgcccag gaatcaaaag
      721 tcgccgttct tccagacaag ccgggaacgg tttttctggt ggtggatttg tacaccagaa
      781 actcacgcca cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagagtctat
      841 cggacagtca gaaatgatca tatccagttt atgctgactt aattgctcca gcagcatttc
      901 gtgggtggat tcgaagcagc gaagatgaat gggctcgcct tctaccactg cggcgttaag
      961 tacgctactg accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt
     1021 ggattctttg cgatagttca caatatccag catttcctgg cttaaggtga acattttatc
     1081 ggcatagcga tagaccagtt ctcccagctc gctgggttcg agaccacgtc ccttgcgttt
     1141 aaataatttg ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa tggtttgtgg
     1201 agttaaataa agcgcctccg ctgcgccaac cacggaacct tctttataga catgccagaa
     1261 gtaatacaag tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga
     1321 aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga
     1381 acgcgtaacc agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt
     1441 ttcgcccagt taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata
     1501 gacatagtaa aaccgatacc gcacaggatc cccaccacca taatttgctg ataagtcgtt
     1561 ccctcaggca gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc
     1621 agcggtttgc caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca
     1681 tccagcgtga cgccttgcag tgaaacgcca gcattagaaa atgcaaacag cggcaaaatc
     1741 agatacgcca cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc
     1801 ttctctttca aaggaataaa gaagccgaca attacccccg ccagagttgc gtgaaccccc
     1861 gatttcaaca ccgcagtcca caacaccacg ccaacaagaa tatagacgcc cgtgcggcgt
     1921 acaccacaca gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagagag
     1981 gccatcgata agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg
     2041 ataatagcca gagccatcaa aaagatcttc agcgctaacg gaacacgact tcccaacagc
     2101 gccagtacac caagtgcaaa agcaatgtca gtagccgccg ggatcgccca cccttcgcgg
     2161 gtaatcggat cggcatagtt aaaaggcaga tagagtaatg ccggcacaat catcccacca
     2221 atagcggcga taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt
     2281 tcacgtttaa cttccagacc gactaacagg aaaaataccg ccatcagcgc gtcatttatc
     2341 cataacagca tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc
     2401 agaaagtcgt gataccatcc actggttgcg ccgctgttgg ccataatcat cgccaggata
     2461 gcggcaatga taagaataat gcctcccgag gcatcactgc taaagaattg atgcagatgt
     2521 ttcacttttt atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa
     2581 tcatcgttaa aatcagatta tagtgtagga ataattcgtt ttttacgagc tattgaagaa
     2641 tttgcgccat cagcggtgag atgcgttttt gacggctgta agcgctcacg agtgacccta
     2701 agcccagata atcatagctt aaggtgagga ttcctgaggg attaattagg aaaagtaata
     2761 attaatttgt tgtcagtcgg cgatgcttgt taggccaata tttcaaaaat aatccagctt
     2821 gtcgtcaata attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat
     2881 tacgccacaa aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa
     2941 gagagcgata taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg
     3001 cagatagaga aaagccccga gtgatatttt accatcaacc cgaggcctcc tatatgctga
     3061 acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc
     3121 ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg
     3181 tgacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt
     3241 tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg
     3301 tgctcgcata tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg
     3361 gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga
     3421 gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag
     3481 ctgtttctgc ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc
     3541 accctgtgca ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc
     3601 ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat
     3661 ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc
     3721 ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg
     3781 tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac
     3841 ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca
     3901 tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg
     3961 tacagcgaag aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt
     4021 ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt
     4081 cggaatgcgg atttctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt
     4141 atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc
     4201 gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc
     4261 catgcctcct tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga
     4321 gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc
     4381 accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc
     4441 acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt
     4501 ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca
     4561 gtataattac ccgtttataa ggcgattatt ttttgtcttt gacttcttca aattcagcgt
     4621 cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct
     4681 gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca
     4741 ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg
     4801 cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct
     4861 tacgggagct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact
     4921 tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt
     4981 tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg
     5041 cggaaacgtg caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc
     5101 cgcgcggtgc cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagccg
     5161 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
     5221 tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca
     5281 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
     5341 cttttacgtc accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt
     5401 ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca
     5461 ttggcatacg agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc
     5521 cagcatcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca
     5581 ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg
     5641 cagtgatgta tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt
     5701 tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct
     5761 gatctttctt gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac
     5821 ccaggtgggt atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt
     5881 cgatttcgat aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac
     5941 ggttgccagt gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga
     6001 tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat
     6061 cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt
     6121 cagcggtttt cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct
     6181 ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga
     6241 tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg
     6301 caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag
     6361 tttcaccatc ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct
     6421 ccagcacgcg aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt
     6481 cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt
     6541 gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag
     6601 actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat
     6661 gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa
     6721 ttgacagcaa ttttattcat ataaagaatg aatcgcagcc aaggcattca tcaaaaaatt
     6781 gtaataaaaa gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg
     6841 tattcatccc cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt
     6901 ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac
     6961 tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg
     7021 tttgtcgcat tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagcg
     7081 gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag
     7141 aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac
     7201 gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc
     7261 tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat
     7321 tacctgaatg attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat
     7381 ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag cgtactgatg
     7441 cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag
     7501 ctgattgccg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     7561 cacggtaaat tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc
     7621 gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat
     7681 acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg
     7741 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt
     7801 gtgtcgcatt tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc
     7861 gtacttgggc atggtttgct gcgcggagcg gggctgggag gccctgtggg cgcagcatta
     7921 aatggggtta aagcggtcag cggcagcgcc tatcgcgtga cgattccagc cgtactgcaa
     7981 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa
     8041 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     8101 gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg atgatgattt
     8161 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     8221 cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat
     8281 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga
     8341 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct
     8401 gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca
     8461 gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac
     8521 tctgaaaggc gcacgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct
     8581 gctggcgatc ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg
     8641 tctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt
     8701 tggtcgcacc gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg
     8761 gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc
     8821 acttctggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     8881 gcaaaaatac catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct
     8941 ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacgccc
     9001 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca
     9061 ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     9121 cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc
     9181 tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca
     9241 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga
     9301 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     9361 aagatttttc cgtttaaaca gtctcgttaa acagaatgat acactgcgaa gggagtgaca
     9421 gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     9481 agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc
     9541 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     9601 atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct
     9661 tcgctctccg ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc
     9721 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     9781 tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga
     9841 gcgtactctt tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc
     9901 gagatcagga acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg
     9961 gtcaggttac agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag
    10021 gtagaagcca gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg
    10081 atcaggcgtt ttgctttcgc aattgacgct tcggtgtcat aggaaagacg cgcatcaact
    10141 tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt
    10201 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
    10261 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
    10321 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
    10381 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
    10441 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
    10501 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
    10561 gcgttgatat cgcccgccat ttttatacaa aacctcatgt atgctacgca gaagttatca
    10621 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
    10681 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
    10741 ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
    10801 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
    10861 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt
    10921 gggtagcggc aacctggccg gcgttgcgct ggcaattacc gccggtggac caggtgccgt
    10981 cttttggatg tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct
    11041 tgcacaactt tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta
    11101 tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat
    11161 cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt
    11221 ttcttttgat tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc
    11281 aatcactcgc ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc
    11341 gataatctgg gtactgacca gcctggtgat ttgcgtaata aatattgggc aacttcccca
    11401 cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc
    11461 gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga
    11521 ggcgggaatg ggatcgacgc caaacgcggc agcggcagcg gcagcggcag cggcagcggc
    11581 agcggcatcc tggcctccgc atccggcagc acaagggatt gtccagatga ttggcatttt
    11641 tatcgacacc ctggtcatct gtacggcaag cgccatgctg atattactgg cgggtaacgg
    11701 cacaacctac atgccgctgg aaggtattca gcttatccag aaggcgatgc gggtgctaat
    11761 gggttcctag ggtgctgaat ttgttaccct cgtggttatt ctgtttgcct tcagctccat
    11821 cgttgccaac tacatttatg ccgaaaacaa tctcttcttt ttacgcctga acaaccctaa
    11881 agcgatctgg tgtttgcgga tctgcacctt cgcaacggtc atcggcggca ccttgctaag
    11941 ccttccgctg atgtggcaac tggcagatat cataatggcc tgcatggcta ttaccaattt
    12001 gaccgccatt ttactgctct cgcctgtggt tcataccatt gccagtgatt atctacgcca
    12061 gcgtaaactc ggcgtgcgcc cggtgtttga tccgttgcgt tatccggata tcggtcgcca
    12121 gctttctccg gacgcgtggg atgatgtttc gcaggagtaa tcacaactat cgatcaactc
    12181 attctctttt tttgctaaag tcggcataaa tttcctgcaa ggactggata tgctgattct
    12241 tatttcacct gcgaaaacgc ttgattacca aagcccgttg accaccacgc gctatacgct
    12301 gccggagctg ttagacaatt cccagcagtt gatccatgaa gcgcggaaac tgacgcctcc
    12361 gcagattagc acgctgatgc gcatcagcga caaactggcg ggtatcaacg ccgctcgctt
    12421 tcatgactgg cagccagatt tcacgccggc gaatgcccgc caggcgattc tggcgtttaa
    12481 aggtgatgtc tacaccggct tgcaggccga aaccttcagc gaagacgatt tcgattttgc
    12541 ccaacagcat ttgcgaatgc tttccggctt gtatggcgta ctgcgcccgc tcgatttaat
    12601 gcagccttat cgtctggaaa tggggatccg tcttgagaat gcccgaggga aagatctgta
    12661 tcaattctgg ggagatatca tcaccaacaa gctgaacgag gcgctcgcag cacaaggcga
    12721 taatgtggtg attaatctgg cgtcagatga gtattttaaa tcggtgaagc cgaagaaatt
    12781 gaatgccgag attatcaagc cggtgttcct cgatgagaag aacggcaaat ttaagatcat
    12841 cagcttctac gctaagaaag cacgcggtct gatgagtcgt ttcattattg aaaatcggct
    12901 gaccaaacca gagcaactga ctggttttaa tagcgaaggt tacttctttg atgaagattc
    12961 ctccagcaat ggcgaactgg tgtttaaacg ctacgagcag cgttaagttt gtagtcaatg
    13021 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca tgcagattca atatattgca
    13081 gaattaacgt aggcctgata agacgcgcca gcgtcgcatc aggcgctgaa tgccggattg
    13141 ttacccggca tttgtcattt agcggtgatg tttgcctgga ccatgaccgc cgtgatgatc
    13201 atgaggagct ttcttatggt ggcgcggcgg tggcggcggt ccgtgtgggt gccagcgatt
    13261 gcctcgccat tcataatgtt gtttccacca gccgtggtcg cgccagtgac ctccatccca
    13321 gtaatagcca cgattatcac gatcgcctat ctgtaatttt actgacggga ctaacgtaat
    13381 ttccgcagcc tgtgctgcca tgggagcgac cagaaccagg gaaagtgcga gtacgataga
    13441 ttgcatcttt ttcacaggtt actcctttta tcattgcccg ttatgggtcg atatccggag
    13501 attacgcgat gagaacgacg gttgttattc tccgcaatcc taatctctaa gttaccgcat
    13561 ttattgataa tttctccttt ttccctgtcg tttttctcca taatttcttc ataaaaaagc
    13621 cgggctgcat aaaagcaaac ccggcctgat tgagataatg aatagatttt actgatgatt
    13681 catcatcaat ttacgcaacg cagaaaaatc ggcgggcaga ttatgtgaaa gcaagggtaa
    13741 atcagcacgt tctgccagct cttttggcag atccaacgtt tcaccgagaa tcgcttccac
    13801 gctctcttta aatttcgccg gatgcgcggt gccgaggaac aagccatatt cgcctggatt
    13861 caactggtca cgcagcgcac gatacgctac ggcagcgtgc ggctccgagg tgtagcccag
    13921 ttcttttaac tcacgcattg tctgttgcgt ggtttcatca tccacggctg cataacccag
    13981 ctctttcagt tgccagattt tgcggcggaa caactcttcc acacgcggcc agttgttcgg
    14041 ctgactcacg tccatcgcgt tggataacgt cgcctgagtc gctttgggtg accactgacc
    14101 gtcgtgcagg aaacgtggca cggtatcgtt cacgttggtc gcagcaataa aacgtttcac
    14161 cggcagaccg aatgacttcg ccagcagacc cgccgtcaaa tcgccgaagt ttccgcttgg
    14221 caccgagaca accagctggt tgcgcgcctc ctgcggcagc tgcgcaacag cttcaaagta
    14281 gtagcaaatc tgcgccagca aacggctgat gttaatcgag ttagccgagt ttaaccccag
    14341 cgccactttc agttcttcat catcaaacgc ctgcttcacc agcgcctgac aggcatcgaa
    14401 atcgccgtcg atggcaaccg tttcgatatt gccgcccaac gtacagaaca gtttttcttg
    14461 cagtggactg attttgcctc gtggatagag gataaccact ttcacattcg gcaaaccgta
    14521 gaaagcatga gccactgcag ctccggtatc accggaggtc gcggtcagaa tggtcactgg
    14581 cttatcatcc gcaatatggg tcagcatttg tgccataaag cgaccgccga aatctttaaa
    14641 tgccagcgtt ggcccgtgga acaattccag acaaccgaca tcgctttcaa cattggcgac
    14701 cggagccggg aaggcaaacg ccgcgcgcac gcgctcttcc aggatttcct gtgggatttc
    14761 atcaccaata aacgccgaga ggatcttcgc actgcgggtg acaaaatcca gcttcagcat
    14821 ctcatcaatt tcagtcaggc tgaattccgg caggtcgtgc ggaaaaaaca gcccctgatt
    14881 tttgcccaac ccctgggtta cggcttgcgc aaagctgacc tgctcgttgt gatctttcag
    14941 attgtagagt ttcatttagt tttccagtac tcgtgcgccc gccgtatcca gccggcaaat
    15001 atgaacaaaa ccttcctgat tttgcaggta gttcttaccc aaccagtcgg caacgcgctg
    15061 ggcggtatcc ggcttgtcac atagagcgaa caaggtcggg ccggagccgg agataccgct
    15121 cgctaccgcg ccgatttccg cgaccgcctg ccgcgcctgc cggaagccag gcagtaaccg
    15181 ttcacggtag ggttcagcga taacatcttt catcagcttc gcggcaagct caggctgacg
    15241 ggaatagcag gcgtgaatga agccagccag atgtcgcccg tgcgcaatgc aatcctggcg
    15301 gcgatactgc gccggtaaaa tagcccgggc ttctgccgtc gagactttaa ttcccggata
    15361 cgccagcacc cacagccact catcaaaccc tggcacttgc tggctgatga tgtcgttttc
    15421 ttcgatcatc aactgcatac caccaagaaa acacggtgcc acgttgtcgt aatgaatgct
    15481 gccggagata cgtccttcca gctcgcccat caaagccagc aaacgagtgt cattaagcgg
    15541 cttgccgcag tgttcattca tcgccatcag cgccgcgacc accgaacagg cgctggagcc
    15601 taagcccgaa ccgatcggca tattcttttc cagagtcatc gccactggaa tttgcttgcc
    15661 aagctcctgg caaaaacgct cccagcactg ataaacgata ttttcccgtg gttctgacgg
    15721 cagcttatcg gcaaagcgtc cgaggttgtt gagactgaat gtctctgccg cctcaaccgt
    15781 gactacatct ccgagcaatg caccatcaac gggtgccacc gccgccccga gcacatcaaa
    15841 cccgacgctc atattggcac tggaagccgg ggcataaact ttaaccatgt cagactccta
    15901 acttccatga gagggtacgt agcagatcgg caaagacacc ggcagcggta acgtcattgc
    15961 ccgcaccata tccgcgcagc accaacggca gcggctgata atagtggcta taaaaggcca
    16021 gggcgttttc gccatttttc actttgaaca gcggatcatt cccatccact tcggcaatct
    16081 tcacgcggca gacaccatct tcatcaatat tgccaacata gcgcaaaact tttccttcat
    16141 cacgggcctt cgccacgcgc gcggcaaaga gatcgtcgag ctgtgacaga ttcgccataa
    16201 aagcggcaac atcaccctcg gcgttaaact ctgcgggcag cacaggttca atttcaatat
    16261 ccgccagctc cagttcacgt cccgtttcac gagcgagaat caatagttta cgcgccacat
    16321 ccataccaga aagatcatct cgcgggtccg gttcggtata acccatttcc cgcgccagcg
    16381 tggtcgcctc ggagaaactc atgccttcgt ctaacttgcc gaagatataa gaaagcgaac
    16441 ctgaaagaat gccggagaac ttcaccaatt catcacctgc attgagcaga ttttgcaggt
    16501 tctcaataac cggtaatcca gccccaacgt tggtgtcata gaggaattta cgccgcgatt
    16561 tttccgccgc ataacgcaac tgatgatagt aatccatcga cgaggtgttg gcctttttgt
    16621 tcggcgtgac aacgtggaaa ccttcgcgca ggaagtcggc atattgatcc gccactgcct
    16681 ggctggaggt gcagtcaaca atgaccgggt tcagcagatg atattctttc acgaggcgaa
    16741 ttaagcgccc gagattaaac ggctctttgg cttgtgcaag ttcttcctgc cagttttcca
    16801 gattaaggcc atgcacacta gtgagcagag ccttcgagtt ggcaacgccg cagacacgta
    16861 agtcgatatg tttattcttc agccagcttt gctgacgctt cagttgctcc agcagcgcac
    16921 cgccaacgcc accgacgcca atcacaaaca cttcgataac ctgatcggta ttgaacagca
    16981 tctgatgagt aacgcgcacg ccagtggtcg catcatcgtt atttaccacg acagagattg
    17041 agcgttcaga agatccctga gcaatggcga caatgttgat attggcgcgg gccagcgcgg
    17101 caaagaattt cgccgagatc ccacgcaagg tgcgcatacc atcacctacc accgagataa
    17161 tggccagccg ttccgtcacc gccagcggct ccagtaagcc ttctttcagt tccaggtaga
    17221 actcttcctg cattgcccgt tcagctcgca cacagtcgct ttgtggaacg cagaaactga
    17281 tactgtattc ggaagatgat tgcgtaatca gcaccacgga aatacgggcg cgtgacattg
    17341 cagcaaagac gcgcgccgcc atgccaacca tccctttcat ccccgggccg gaaacgctga
    17401 acattgccat gttattcaga ttggaaatgc ccttgaccgg taattcgtct tcatcacggc
    17461 tggcaccaat gagcgtacct ggtgcttgag gatttccggt atttttaatc aggcaaggga
    17521 tctggaactg ggcgatgggg gtaatggtgc gggggtgaag aactttagcg ccgaagtagg
    17581 aaagctccat cgcttcctgg taggacatcg acttcaacaa cctcgcatcg ggcacctgac
    17641 gcgggtcgca ggtatagacc ccgtcaacgt ccgtccaaat ctcgcaacaa tcggcgcgta
    17701 aacaggcagc cagcaccgcc gcggagtagt cggaaccgtt acgtccaagc accaccagtt
    17761 cgcctttttc attaccggcg gtgaaacctg ccatcaacac catgtgatca gccggaatgc
    17821 ggcttgccgc aatacggcgg gtggactcag caatatcgac ggtagattcg aggtaatgcc
    17881 ccactgccag cagtttttcg accggatcga taacggtaac gttgtgacca cgcgcttcta
    17941 acacgccggc cataatggcg atcgacattt tctcgccacg gcaaatcagc gcagcgttga
    18001 tgctatccgg gcactgcccc aacaaactaa tgccatgcag gacatgtttt atttgggcaa
    18061 attcctgatc gacgaaagtt ttcaattgcg ccagcgggaa ccccggctgg gcggcggcga
    18121 gtcccgtcaa aagttcggca aaaatacgtt cggcatcgct gatattgggt aaagcatcct
    18181 ggccgctaat ggttttttca atcatcgcca ccaggtggtt ggtgattttg gcgggggcag
    18241 agaggacggt ggccacctgc ccctgcctgg cattgctttc cagaatatcg gcaacacgca
    18301 gaaaacgttc tgcatttgcc actgatgtac cgccgaactt caacactcgc atggttgtta
    18361 cctcgttacc tttggtcgaa aaaaaagccc gcactgtcag gtgcgggctt ttttctgtgt
    18421 ttcctgtacg cgtcagcccg caccgttacc tgtggtaatg gtgatggtgg tggtaatggt
    18481 ggtgctaatg cgtttcatgg atgttgtgta ctctgtaatt tttatctgtc tgtgcgctat
    18541 gcctatattg gttaaagtat ttagtgacct aagtcaataa aattttaatt tactcacggc
    18601 aggtaaccag ttcagaagct gctatcagac actctttttt taatccacac agagacatat
    18661 tgcccgttgc agtcagaatg aaaagctgaa aaatacttac taaggcgttt tttatttggt
    18721 gatatttttt tcaatatcat gcagcaaacg gtgcaacatt gccgtgtctc gttgctctaa
    18781 aagccccagg cgttgctgta accagtcgac cagttttatg tcatctgcca ctgccagagt
    18841 cgtcagcaat gccatgactc gttcgcgtaa agcttgcagt tgatgttggt ctgccgttgt
    18901 gtcacttttc gccggttgtt gtattaatgt tgctaattga tagcaataga ccatcaccgc
    18961 ctgccccaga ttgagcgaag gataatccgc caccatcggc acaccagtaa gcacgtcagc
    19021 caacgctaac tcttcgttag tcagcccgga atcttcgcga ccaaacacca gcgcggcatg
    19081 gctcatccat gaagattttt cctctaacag cggcaccagt tctactggcg tggcgtagta
    19141 atgatatttc gcccgactac gcgcagtggt ggcgacagtg aaatcgacat cgtgtaacga
    19201 ttcagccagt gtcgggaaaa ctttaatatt atcaataata tcactagatc catgtgcgac
    19261 ccagcgggtg gctggctcca ggtgtgcctg actatcgaca atccgcagtt cgctaaaacc
    19321 catcgttttc attgcccgcg ccgctgcccc aatattttct gctctggcgg gtgcgaccag
    19381 gataatcgtt atacgcatat tgccactctt cttgaccaaa taaccgcgaa cagggtgatc
    19441 actgtcaact tattacgcgg tgcgaattta caaattctta acgtaagccg cagaaaaagc
    19501 cctttactta gcttaaaaaa ggctaaacta ttttctgact gcactaacgg tttaattgtt
    19561 aaaaaatgct acatatcctt ctgtttactt aggataattt tataaaaaat aaatctcgac
    19621 aattggattc accacgttta ttagttgtat gatgcaacta gttggattat taaaataatg
    19681 tgacgaaagc tagcatttag atacgatgat ttcatcaaac tgttaacgtg ctacaattga
    19741 acttgatata tgtcaacgaa gcgtagtttt attgggtgtc cggcccctct tagcctgtta
    19801 tgttgctgtt aaaatggtta ggatgacagc cgtttttgac actgtcgggt cctgagggaa
    19861 agtacccacg accaagctaa tgatgttgtt gacgttgatg gaaagtgcat caagaacgca
    19921 attacgtact ttagtcatgt tacgccgatc atgttaattt gcagcatgca tcaggcaggt
    19981 cagggacttt tgtacttcct gtttcgattt agttggcaat ttaggtagca aacatgcaga
    20041 ccccgcacat tcttatcgtt gaagacgagt tggtaacacg caacacgttg aaaagtattt
    20101 tcgaagcgga aggctatgat gttttcgaag cgacagatgg cgcggaaatg catcagatcc
    20161 tctctgaata tgacatcaac ctggtgatca tggatatcaa tctgccgggt aagaacggtc
    20221 ttctgttagc gcgtgaactg cgcgagcagg cgaatgttgc gttgatgttc ctgactggcc
    20281 gtgacaacga agtcgataaa attctcggcc tcgaaatcgg tgcagatgac tacatcacca
    20341 aaccgttcaa cccgcgtgaa ctgacgattc gtgcacgcaa cctgctgtcc cgtaccatga
    20401 atctgggtac tgtcagcgaa gaacgtcgta gcgttgaaag ctacaagttc aatggttggg
    20461 aactggacat caacagccgt tcgttgatcg gccctgatgg cgagcagtac aagctgccgc
    20521 gcagcgagtt ccgcgccatg cttcacttct gtgaaaaccc aggcaaaatt cagtctcgtg
    20581 ctgaactgct gaagaaaatg accggccgtg agctgaaacc gcacgaccgt actgtagacg
    20641 tgacgatccg ccgtattcgt aaacatttcg aatctacgcc ggatacgccg gaaatcatcg
    20701 ccaccattca cggtgaaggt tatcgcttct gcggtgatct ggaagattaa tcggctttac
    20761 caccgtcaaa aaaacggcgc tttttagcgc cgtttttatt tttcaacctt atttccagat
    20821 acgtaattca tcgtccgttg taacttcttt actggctttc attttcggca gtgaaaacgc
    20881 ataccagtcg atattacgag tcacaaacat catgccggcc agcgccacca ccagcacact
    20941 ggttcccaac aacagcgcgc tatcggcaga gttgagcagt ccccacatca ctccatccag
    21001 caacaacagc gcgagggtaa acaacatgct attgcgccag cctttcaata ccgcctgcaa
    21061 ataaataccg ttcattaacg ccccaatcag actggcgatt atccatgcca cggtaaaacc
    21121 gatatgttca gaaagcgcca gcaagagcaa ataaaacatc accaatgaaa gccccaccag
    21181 caaatattgc attgggtgta aacgttgcgc ggtgagcgtt tcaaaaacaa agaacgccat
    21241 aaaagtcagt gcaatcagca gaatggcgta cttagtcgcc cggtccgtta attggtattg
    21301 atcggctggc gtcgtcactg cgacgctaaa cgcggggaag ttttcccagc cggtatcatt
    21361 gcctgaagca aaacgctcac cgagattatt agcaaaccag ctgctttgcc attgcgcctg
    21421 aaaacctgac tcgctagctt cccgtttggc tggtagaaaa tcacctaaaa aactgggatg
    21481 cggccagttg ctggttaagg tcatttcgct attacgcccg gcaggcacca cagaaagatc
    21541 gccggtaccg cttaaattca gtgccatatt cagctgcagg ttctgcttcc gccagtcccc
    21601 ttcgggtaaa gggatatgca cgccctgccc gccttgctct aacccggtac cgggttcaat
    21661 ggtcagtgcc gttccgttaa cttcaggcgc tttcaccaca ccaataccac gcgcatcccc
    21721 gacgctaatc acaataaagg gcttgcctaa gatgatattt ggcgcgttga gctcgctaag
    21781 acgcgaaaca tcgaaatcgg cttttagcgt taaatcactg tgccagacct gaccggtata
    21841 aatccctatc ttgcgttctt ccacgttctg attgccatca accatcaatg actcaggtaa
    21901 ccaaaaatgg ataaaacttc gtttccgctc cacggtttta tcctcttcct gcaccgtata
    21961 aagctcggtc acaggaatag cgataagcgg cccaacgagt ttttgcggcc cgctggtact
    22021 ttggcgaatc gcatcttcca catcgctacg gtaatcggca cgttcgacaa tcacttgccg
    22081 aatcagcatt atcggaatca acaacagcaa tactgcacca aacaaggtgg tcattttcca
    22141 gaacaggggg gatttcaaca tagtcttctc ctttgcaatg gcagcaggat acgaaggcta
    22201 tgtgggaaga atttgaagct atgtgaagtt acggtgaagt cgtagcgagg ccagcacgcc
    22261 accttcctgc acgttgcgca gcgtgacttc gccgttaaac aaacgggcga cctcactgac
    22321 gaacgccaac cccagaccgc tgcttttttg cccatttgca cgcggcaaag agtaaaagcg
    22381 ttcaaaaata cgtgacagcg cgtagtcagg aataccacta ccggtatcca gcaccttaag
    22441 cgtgacgtgt tcctgatcca cttcggcgct tagcgttatg caaccgctct cgggagtaaa
    22501 atcgatggtg ttatccagta aattccccag cgcctgatcc agtaacgccg gttcagcagc
    22561 aacattaacc tcggtgggca taacatgcaa agtgatgttt ttttctgcca actgcacggt
    22621 gcgcgcttcg ctgacgcggc gaaataatgc cgccacatca acagcagtca gaacgacttc
    22681 ctgacgattc tccagtcttg cctggcgtag taacgtttcc accagcgcct gcattcgcgc
    22741 attttgtgtc agaatgttgt cagtaaaacg agccactact tcaggcggcg gaccttcgcg
    22801 taaaatttcc gccacgccac gaatcgccgc cagtgggctt tttagctcat gggttaacgc
    22861 ataaacatac tgctcaatat agtttttccc ttccagcttc acgcgcatac tttccagcgc
    22921 ctgcgcgagt ttacgcagct cgctactacc gagatcggga agaggaacgg gcttattgtc
    22981 agtgacggaa tcagcatagc gagtgagccg ggcaatggag cggttgatcc accaaaccat
    23041 gcctgcgcca atcaccagtg caatccccaa caaaatggcg ctggcccata aaattcgccg
    23101 ctcgctgcgt ttaatgaccg gagccatcgc cgcgttcggt ttgcctacgc tcaaaacgcc
    23161 aataagccgc gagccgccca taatcggcgc ggcaacatac atcaccgaac tttcgggatc
    23221 ggcaggattt tgcaacgtgc tgcgcgcacc atactgacca cgcaacgtta gccagacgtc
    23281 attccagcgc gaataatcct gtccaacggc tttatttgcc gaatcgaaca atactttgcc
    23341 atgcgcatcg gtcatataga catgatattc attgcgcact ttgttaatgc caccaatatt
    23401 ggcgcgaaac gggcgatgtt gtagctgatt aaacgcctgc gccagttgcc catgcgttgg
    23461 gtccccagag agcaaatcgg gacgcgccag ctccgcaagt aacgttgccg tgtcgatcaa
    23521 cgtcccctcc gttgctcttc gcacgcccgg tttaacttct ttgacaaaaa tagccagtac
    23581 gaaccaggcc gccaccgcca ccagtaaaaa atagcccagc agcaaccgca tgccgatacg
    23641 cattacaagc ccctcaggct atatcccatg ccgcgatgag tattaatcgg tgaaagatcg
    23701 gggttgatgg cgcgcagctt ggcacgcagc gttttaatgt ggatatcgac ggtgcgatcg
    23761 taggtgtcct gcgcatcttc ccacacgcta tccattagtt gctggcggga ccagacgcgg
    23821 cctagcgact tgagtaacgt cttcagcaat aaaaactcat accgcgtcag cgttaatggc
    23881 gtgtcaaacc agctgatctg cgccgcgggt tcattcaatt caaaatgacc aatacggatg
    23941 acgggagacg gcgtcgagaa cttcttcacc cgacgcagta aggtgcgcac cctggcgcac
    24001 acttcgcggg gtgaaaacgg tttagccaca tagtcgtcag cgccaatttc cagcccaagc
    24061 aggcgatcga cctcttcact tcgggccgtc aggaacagta caggtaacgc cggatggagc
    24121 gccagtaact ggcggcacaa ttcaaagcca ctgatatcag gcaaaccaac gtcgagaatc
    24181 atgacgtcgg gaacctgctg gcgagcttta tccagcaccg gcaaacctcg ctcaaagacc
    24241 tcgacggcaa aaccttcctg ctgcaacatg tagaccagcg tgtcggctat cccttgctca
    24301 tcttccacta accagaccgt ttcccgttgc ataaatcgcc tctgttattg ccgccacggc
    24361 atgacaggaa ccgcgctaat cgcgtttttc ggcgaacctt ctacaacttt gtcggagtaa
    24421 gccagataag cgagcgcgtt gcgtttggcg tcataaaagc gcacgacctg taacgactta
    24481 aagacaaggg acgtgcgttt tttgaatact acctcgccct gagctttgcc gtttttaata
    24541 cgatccgaca gttcaatcgg cccgacttgc tgacaagaaa tggccgcatc ggaggtatct
    24601 tctgccaggc ccaatccccc tttaatacca ccggttttcg cccggctcac ataacaggtg
    24661 acatttttca catcgggatc gtcaaaggct tccacaacaa ttttgtgatc cgggccgatc
    24721 attttaaata cggtgtcgac cgaaccaatc tcttctgcat gagccaatgg ccccagcatt
    24781 atcaggctta aagacaggat caaatgcttg tatttcatat tgttaccatt tttatctatt
    24841 acacgagcaa ttagttcgtc acggtaaata acatattgat taagaacaaa atctcaattc
    24901 ttttatttcc gtcaggccct aaaacatact ctactaagaa aaaaacactg aatgctaaaa
    24961 cagcaaaaaa tgctattatc caattacctg atgtcaggtg ctcgttgttg aaaggatgag
    25021 gatattttat ggatcaggcc ggcattattc gcgacctttt aatctggctg gaaggtcatc
    25081 tggatcagcc cctgtcgctc gacaatgtag cggcgaaagc aggttattcc aagtggcact
    25141 tacagagaat gtttaaagat gtcactggcc atgctattgg cgcgtatatt cgtgctcgtc
    25201 gtttgtcgaa atcggcggtc gcactacgcc tgactgcgcg tccgattctg gacatcgcgc
    25261 tgcaataccg cttcgactct caacagacat ttacccgcgc attcaagaag cagtttgccc
    25321 agactcctgc actttaccgc cgttctcctg aatggagcgc ctttggtatt cgcccgccgc
    25381 tacgtctggg taaattcaca atgccagagc acaaatttgt caccctggaa gatacgccgc
    25441 tgattggtgt tacccagagc tactcctgtt cgctggagca aatctctgat ttccgccatg
    25501 aaatgcgtta tcagttctgg cacgattttc tcggcaacgc gccgaccatt ccgccggtgc
    25561 tctacggcct gaatgaaacg cgtccgagtc aggataaaga cgacgaacaa gaggtattct
    25621 ataccaccgc gttagcccag gatcaggcag atggctatgt actgacgggg catccggtga
    25681 tgctgcaggg cggcgaatat gtgatgttta cctatgaagg tctgggaacc ggcgtgcagg
    25741 agtttatcct gacggtatac ggaacgtgca tgccaatgct caatctgacg cgccgtaaag
    25801 gtcaggatat tgagcgatac tacccggcag aagacgccaa agcgggagat cgcccaatta
    25861 atctgcgctg tgaactgctg attcctatcc gccgttaacg ctgcagctca tctaatgcag
    25921 gggcgtccag atgcgagatg tcccctgcgg tttcaacgac ccagccagac gccagccaca
    25981 ggctttcctg ataatccacg cgcgaaatag aacagttacg cagacgtaag cggcgctctg
    26041 cccatgctgg taatccgaga atcgtactca ccagacatcc cagtgcaata ccatggctca
    26101 ccagcagcgg tcggcttccc tgcggtaagt cccggcaaga ttccagtgcc gcattgacgc
    26161 gatcgctgag ctcctgcatt gactcgcctt caggaatacg cccgtcaacg gtgccattga
    26221 ccagctgccg acgccagttc tcttcttctt cggtcagaga atcgatatgt cttttttcca
    26281 gcacacccat gtttaattca cgcaggcgag aatcaaagat gatgtcacag ccgcaggcct
    26341 gggcgatgat ttccgccgta cgccgggtgc gtcctaaatc gctactgata atatgcgtaa
    26401 tgccaagctc tttggcacgg gttgccacct gcatcgcctg ttgctcacct ttggcggtca
    26461 gcgggctgtc agactggccc tgaatacgtc gctcggcgtt ccactgcgtt tcaccgtggc
    26521 ggactaggta tacctgtaac atgctttttt tccgtatact gctgtaacgt taattttctg
    26581 agtaatgctg attatgcacc aagttgtctg tgcgaccacc aatcccgcta aaattcaggc
    26641 cattctgcag gcatttcacg agatcttcgg cgaaggatcc tgccatattg catccgttgc
    26701 cgtcgagagc ggtgtaccgg aacaaccctt tggcagtgag gaaacgcgcg ctggcgcacg
    26761 aaatcgggta gcaaatgccc gccgtttact tccagaggct gatttttggg tggcgattga
    26821 agctggcatt gatggcgaca gcactttcag ttgggtggtg attgaaaacg ccagccagcg
    26881 cggcgaggcg cgttcggcaa ctttgccgtt acctgcggtg attctggaaa aagttcgtga
    26941 aggcgaggcg cttggtcccg tgatgtcgcg ttataccggt attgatgaga ttggccgtaa
    27001 ggaaggggcg atcggcgtgt ttaccgccgg gaaactcact cgcgccagcg tctatcacca
    27061 ggcggtgatc ctggcgctga gtccgtttca caatgccgtg tatcaggcat tgtaggacgg
    27121 ataaggcgtt cacgccgcat ccgacattcg gtgcacgatg cctgatgcga cgctgccgcg
    27181 tcttatcagg cctacaaaat caatcgcttt tcagcaacac ctcttccagc cactgacgca
    27241 gctccacggg cgcggctttc aggctgttag atccacgcgt aatcgtcgcg atgcccgcgc
    27301 cgagttcatt ttttaactca cgctgactca tttcgccacg caacagctct tcgacaatac
    27361 gcacgcgagt ccccaacgct tcgcgctcat ctggcgtcag catcaggttt aacaacggta
    27421 aatggagatc gttttggtag gcattcttaa gcaggtcgac aaaacgtaac cactcctggt
    27481 gacgctgttc tgccatcgct gctgaatagg gtgattgttg ggccataata tgtcgccatt
    27541 gttagcgggg gaagcaaaat gactcccccg gttgtactcg ctaaagagta cgatagcata
    27601 tcataaacgt gcggatcagt aacgacgtcc ccattccgtg gcgctcatca acgtcggttt
    27661 atcccccatg aaatagcggt agtaagcatc ataagccagc acgttcttca cataaccgcg
    27721 cgtctcggag aacggaatac tctcgacaaa tgccacagcg tcgatacgcc cggcgctgtt
    27781 gccaagccag gttcgcaccc gtccaggtcc ggcgttataa gctgctgagg agaaaatacg
    27841 gttattgcca aactgctgat aaacatattg caggtaactg gtgccaatgt tgatgttcgt
    27901 ttccggatcc agcaattgcc cagggctgct ataaccagga atagagaaca tcttcaccgt
    27961 atgggtcgct gtaccaggca taatctgcat caggccgctg gcccctaccg gtgatttcac
    28021 tttcggattc caggcgctct cctgacgggc aatcgccatc gcatagcttt gcgggatctc
    28081 tttcccactg gtgtaacgtt tgaaaagatc gttgtaagcc agcgggaatc gctcttccag
    28141 atgatcccaa agcttcccgg cgatcgttgc ctgaacgcta agatcccacc attggttatt
    28201 gaaagcatac cgagccagtt gagcctgctc tgtttttgac ttgctcttca ccagattggc
    28261 ccactcgcta cgcgcggtat tatcgagatt ccagtacatc aactcgcgca cgcgcgccat
    28321 ctccggcccc tgagtcaggg tgctgtcaac attctgcggc gctttatcaa tcttcagctc
    28381 atactcttca ccgatgcgtt gtgctgcaac catcgggtag aaaccacgct gttgcatgag
    28441 ttgatgcaaa atctctttcg cttcagcttc acgtccgcgt tccagcaata aatccgcctg
    28501 ccagtaacgc cattcatctt tctcttttgc ttccatcggc agacgcgcca gccaggtatt
    28561 caggccgcga cgatcgccgg tgccaagcgc cattcgcaca cggcgttcaa tcaacgacgt
    28621 ggattgcgag cgcataatgg cgtcatcgcg ccatttcgcc tgctcatcgg tgacatcatt
    28681 gcccatcaaa cgccaggcga cgatatcgcg cagctcctga atctcatctt cattaagctg
    28741 ctgcgcctgg gcaagcgacg ggatcatcag ccgtgcattt tcggcatcct gccgcgccac
    28801 actggcaaac gccaccgccg ccatttgacg ggtaaaatcg gtcgcgccgg ttgtacgcgc
    28861 gaaggtcagt accgtattag ggttgttcgc cagtgaaatg attgccgagg cgatagtctg
    28921 gtaatcggca ggcatctgcc ctgccagcac tgttaccagg cctgtgttac ccgctttcat
    28981 cgccagacgg atacgctcta aatacgccag cggatcttgt ttacctgacg cacgccagac
    29041 gctaaataac ttgtcacagg cgttaggctg gctcttgccg gttagccaca gctctttcgc
    29101 cccttgccag gcttcttcac tctgcccggt gttccatttc gcatagtagt aattacattg
    29161 cgcttcggta gttccgggct tttccgggct aaaggctaac aagccacgcc agtcttcacg
    29221 ccgcgccagt tcattgacga aacgagattg cagcgtgcga gcgggaggaa gcgtggggtt
    29281 agcgcgaaca aagttagtga ccgtcaccgc cggttgattc atcaggtcat cggtgatctg
    29341 gcggtattcc aggtagggat aaagcggata atccttcagt ccaggcatca tttgttcgac
    29401 cacatccatc tgtcgattat cccaggcctg cttgatttgc gcgtaacggc tacgctgctc
    29461 atccagtgag tcggctcgcg ccacgctgct gaccgtcagc agacagacac cggcagccaa
    29521 cagccgccag gtaacttgtt tggctttttc cacaagcgca tcctctaagt gtaaatacat
    29581 caatgcagcg tcataccgcg taatagattt atgctaacca gtcattgccg tttacgccac
    29641 gttacggaca cttttttact tttactgcga aggtgatcga ccaactggga ttaggcgggg
    29701 aaacaggcta cacttcgcct ttgaaaactc atcatcacga taaacataga ggcgaagtcc
    29761 aacgtggctc aattcgttta taccatgcat cgtgtcggca aagttgttcc gccgaaacgt
    29821 catattttga aaaacatctc tctgagtttc ttccctgggg caaaaattgg tgtcctgggt
    29881 ctgaacggcg cgggtaagtc caccctgctg cgcattatgg cgggcattga taaagacatc
    29941 gaaggtgaag cgcgtccgca gccagacatc aagattggtt atctgccgca ggaaccgcag
    30001 ctgaacccgg aacacaccgt gcgtgagtcc attgaagaag cggtttcaga agtggttaac
    30061 gccctgaaac gcctggatga agtgtatgcg ctgtacgccg atccggatgc cgattttgac
    30121 aagctggccg ctgaacaagg ccgtctggaa gagatcattc aggctcacga cggtcataac
    30181 ctgaacgtac agctggagcg tgcggcggat gcgctgcgtc tgccagattg ggacgcgaaa
    30241 atcgctaacc tctccggtgg tgagcgtcgt cgcgtggcgt tgtgccgcct gctgctggaa
    30301 aaaccagaca tgctgctgct cgacgaaccg accaaccacc tggatgccga atccgtggcg
    30361 tggctggaac gcttcctgca cgacttcgaa ggcaccgtgg tggcgattac tcacgaccgt
    30421 tacttcctcg ataacgttgc aggctggatc ctcgaactgg accgcggtga aggtattccg
    30481 tgggaaggta actactcctc ctggctggag cagaaagatc agcgtctggc gcaggaagct
    30541 tcacaagaag cggcgcgtcg taagtcgatc gagaaagagc tggagtgggt acgtcaggga
    30601 actaaaggcc gccagtcgaa aggtaaagca cgtctggcac gctttgaaga gctgaacagc
    30661 accgaatacc agaaacgtaa cgaaaccaac gaactgttta ttccacctgg accgcgtctg
    30721 ggcgataaag tgctggaagt cagcaacctg cgtaaatcct acggcgatcg cctgctgatt
    30781 gatgacctga gcttctcgat cccgaaaggg gcgatcgtcg ggatcatcgg tccgaacggc
    30841 gcgggtaaat cgaccctgtt ccgtatgatc tccggtcagg aacagccgga cagcggcacc
    30901 atcactttgg gtgaaacggt gaaactggca tcggttgatc agttccgtga ctcaatggat
    30961 aacagcaaaa ccgtttggga agaagtttcc ggcgggctgg atattatgaa gatcggcaac
    31021 accgagatgc caagccgcgc ctacgttggc cgctttaact ttaaaggggt tgatcagggt
    31081 aaacgtgttg gcgaactttc cggtggtgag cgcggtcgtc tgcatctggc gaagctgctg
    31141 caggttggcg gcaacatgct gctgctcgac gaaccaacca acgacctgga tatcgaaacc
    31201 ctgcgcgcgc tggaaaacgc cctgctggag ttcccgggct gtgcgatggt tatctcgcac
    31261 gaccgttggt tcctcgaccg tatcgccacg cacatcctgg actaccagga tgaaggtaaa
    31321 gttgagttct tcgaaggtaa ctttactgag tacgaagagt acaaaaaacg cacgctgggc
    31381 gcagacgcgc tggagccgaa gcgtatcaag tacaagcgta ttgcgaagta atgcgtaaaa
    31441 tgccggatgc ggcgcgaacg ccttatccgg cctacaaaac cagcaatttc aatacgttgt
    31501 aaaactgtag gcctgataag acgcggtaag cgtcgcatca ggcattgagc accgaatgcc
    31561 ggatgcggcg tgaacacctt ttccggtcta caacagcctt agcatcctca atttcgtggc
    31621 gggtcacccg tgcaggtttt ttcaaacctt gtttaatctc gacagcctct tccagagagg
    31681 ttttcaactc atcaaaaaaa ctcattttat acctcgcctt tgagcagttt cgtcacggtt
    31741 atccttgctc ccccatcatc tcccgcacca gctccacgca gcgcaggaaa cggctgtcgt
    31801 aatcgtcctc ttcaacccgc acgaattcga tattgttctc ttccagcatc tccaccagca
    31861 agttctggaa ctctttgcga tccaccgaac tgccgaggct gcgtaaacca tccgccaccc
    31921 acggcgtgtt gttctccagc aggatcacca gatcgaaacg gtattcatca atcagcgcct
    31981 gcacgaacgg atgctcacgc ccttcgtact ttttgcagaa cgcctgggta gtgacaaaat
    32041 cggtgtcaat aaacgccact ttattggcgt atttcactgc aaaatcaatg tattgtgcgt
    32101 ggcccaacgc gattttatcg tagtcggaat actgcaatgc gatctcatca ccgccgaggt
    32161 gtgaaaagac ataatcgcgg ccatattccc acgcactggt ggtgttgaag atattggcaa
    32221 gtttgtttac cagggtggat ttaccgctcg actcgccgcc aaggatcgcc acggtacgca
    32281 caaaaaacgg cttcacttcg gtaggaatat attcccagta gcggaacggg ttttcgcgga
    32341 tctgcgcacc gctgatactc ataaaggtac gtttcggatc gaccagcacc gtctcgatcc
    32401 ccagatgttc catatactgt ggcgcatcgg cttcttccga ggtgtagatc aggtccggct
    32461 ggatcccctt ttcagccata aactttttga tgccgttgct ccacacatcc cagccatgcg
    32521 gatacggctc catgccctct tcgttgaaag catgaatacg gatatttttc tgatatttaa
    32581 aggtttgcaa tagccagcgc agacgatccg gcaccgtggg ctgctgcgac atggcgctgt
    32641 cttcaaacaa cgcgcggtcg cgggtatcgt caaaacccat aatgatatgc agctcgtcaa
    32701 cctggctaca ggcgcgctgg ataaggtaga tatgtccggt atgcagcggg tagaacttac
    32761 cgaatacgac accaatggtt ttcttctgcc ggggaaattc aagccccaaa aaacggtgca
    32821 atgcctccag cttttgcgcg ctggggcttt taattttggc attcagtaac tggcttaaat
    32881 accctttggt cataccgctg gcatcagcta cctgctgtag cgtgcagccc tgttgcttga
    32941 tggcagtttt caggtaatca aatgacgaca tatctccctc cgtatctctc attataagtc
    33001 gtcgaacacg ctaagcgcgt cggagagttt tttaacgcca aaaatctgca tcccttccgg
    33061 cgcttttttc ggtacgttag ccgccggaac aatcgcccgg cgaaaaccgt gtttcgccgc
    33121 ttcagaaatc cgctcctggc cactgggcac cgggcggatc tcccctgcca gcccgacttc
    33181 accaaacacc accagatcct gtggcagcgg tctgtcacgc aggctggaaa ccatcgccag
    33241 cagtaacgct aagtcggcgc tggtttcggt taccttcacg ccgccgacca cgttcacaaa
    33301 cacatcctga tcggccattt gcagaccacc gtgacggtgc aagacagcca gcaggattgc
    33361 cagacggttt tgttccagcc ccactgccac gcggcgcgga ttcgccatta tcgagtgatc
    33421 gaccagcgcc tgaatctcca ccagcaacgg acgcgttcct tcccacacca ccatcacgga
    33481 gctgccggag gtcacttcat ctccgcgact taagaaaatt gccgaagggt tgctgacttc
    33541 acgcagcccc tgttcggtca tcgcgaagac gcccagctca ttcaccgcgc cgaagcggtt
    33601 tttatggctg cgtaaggtgc gaaaacgaga gtcggcatcg ccatccagaa gcaccgaaca
    33661 gtcgatacag tgttccagca ctttcgggcc agccagcgaa ccatctttgg ttacgtgtcc
    33721 aaccatgaca atcgccacac cgcgcgtttt ggcgaaacgc gtcagataag ccgccgtttc
    33781 acgcacctgc gccacgctac caggcgatga ttgtacatcc gccatatgca tcacctggat
    33841 cgagtcaatt accatcagct tcggttgctc ttcttcggca atcaggcaga tctgctcgat
    33901 gctggtttcc gacaacatat tgagattgtc agtcggcagg ccaaggcgat gagcgcgcat
    33961 tgccacctgt tgcagcgact cttcgccggt gacatacagc gttttcatct gctgggccag
    34021 tttgcacagc gtttgcagca acagcgtgga tttccccgca ccagggttac cgccaatcag
    34081 aatggcactt cctggcacca cgccgccgcc tagtacgcgg tcgaactctt taaatccggt
    34141 ggaaaaacgc ggcagctctt caaggctgat atcggagagt ttctggactt ttgccacccc
    34201 ggcgctaccg gcatagccgc tgagacgctc gttacgcgcc accgttggcg acgcagcaag
    34261 acgcacctcg gtgatggtgt tccaggcatg acaggcactg cactgcccct gccagcgcgg
    34321 ataatcggcc ccgcattcat tacaaacaaa ggcgcgtttt ggagcttttg ccacggtgta
    34381 cctcgttaat gctgtgccgc ccgcaggatg acgggcaggc aattacttct gattcaggct
    34441 gccagagagg atgcagaata cccccatcag gtcagcgtga cggatggtga cttccgcctt
    34501 ttcattcact tttggcttgg catggtaggc aatccccagc cctgctgctt tgatcatcgg
    34561 caggtcatta gctccatcgc caatcgccac ggtctgcgcc agcgggattt catactcctg
    34621 cgcgaggcga gtcagagttt tcgctttgta ctgtgcgtct acgatgtcgc cgatcacatt
    34681 gccggtaaat ttaccgtcca tgatctccag ttcattggct accacggctg tcaggcgcag
    34741 cttgtcgcgc aggtattcag caaagaaagt aaagccgccg gaggcaatcg ccactttcca
    34801 gcccagcgtt tccagcttga gcaccagctg cgttaagcct ggcatcagcg gcagattttc
    34861 acgcacctgt tgcagaatat tggcgtcagc gcctttcagc gtcgccacgc ggctgcgcag
    34921 gctggcggta aaatcgagtt cgccgcgcat cgcccgttcg gttacctccg ctaccatctc
    34981 gcccgttccg gccagcttgg caatttcatc aatacattca atctggatgg cggtggaatc
    35041 catgtccatc accagcaaac ccggcgtgcg caggtgcggg attttcccca gcggggcgac
    35101 atccagctgc gcttcgtggg ccaggcgtgt agcccgtgcg gtgagtgaac ctgccagacg
    35161 aatcacctga taatcttcca cgcaccaggc ggcaacaatc accatcgccg cacccagttt
    35221 gctctggtat tgggtcagac gctgtttatc cagcccacga ccatacagca gccagccgct
    35281 acgagctgcg tggtaatcca gtggcatcac ttcatcacca cttaatgaaa gaggcagacc
    35341 cggccataaa gagacatctt caggcaggtc gcaccaggta atgttaggca ttaaggctcc
    35401 tgtaaaatcg ttcgaagcag ggaaaataac gcatgaggct accttgtatc cattgcttct
    35461 ggcaacatta agtctcaaat tttcaaaggg tggaagatgg ctcgcacaaa actgaaattc
    35521 cggctacatc gggcagtgat tgtcctgttc tgtcttgcct tgttagtggc actgatgcag
    35581 ggagcgtcat ggtttagtca aaaccaccag cgacagcgta atccacagct ggaagaactg
    35641 gcccgcaccc tggcgcgtca ggtgacgctg aacgttgcac cgctgatgcg taccgactca
    35701 ccggatgaaa aacgcattca ggcgatcctc gatcagttaa cggatgaaag ccgtatcctc
    35761 gacgcgggtg tgtatgacga acaaggcgat cttatcgcac gttctggcga aagcgtcgaa
    35821 gtgcgcgatc ggctggcgct cgacggtaaa aaagcaggcg gctattttaa ccagcagatt
    35881 gtcgagccaa ttgcgggtaa aaacggaccg ctcggctatc tgcgcctgac actcgacacc
    35941 catacgctcg ccaccgaagc ccaacaggtg gataacacca ctaacatttt acgcctgatg
    36001 ttgctgctct cactggcaat cggcgtagtg ctgacccgca cgctgctaca gggtaaacgc
    36061 acccgctggc agcaatcgcc cttcctgtta accgccagca aaccggtgcc ggaagaggaa
    36121 gaaagcgaga aaaaagagtg acccattact acaagaaagg aaatcgttat gtccacatta
    36181 cgcctgctca tctctgactc ttacgacccg tggtttaacc tggcggtgga agagtgtatt
    36241 tttcgccaaa tgcccgccac gcagcgcgtt ctgtttctct ggcgcaatgc cgacacggta
    36301 gtaattggtc gcgcgcagaa cccgtggaaa gagtgtaata cccggcggat ggaagaagat
    36361 aacgtccgcc tggcgcggcg cagtagcggt ggcggcgcgg tgttccacga tctcggcaat
    36421 acctgcttta cctttatggc tggcaagccg gagtacgata aaactatctc cacgtcgatt
    36481 gtgctcaatg cgctgaacgc gctcggcgtc agcgccgaag cgtccggacg taacgatctg
    36541 gtggtgaaaa ccgtcgaagg cgaccgcaaa gtctcaggct cggcctatcg cgaaaccaaa
    36601 gatcgcggct tccaccacgg caccttgcta ctcaatgccg acctcagccg cctggcaaac
    36661 tatctcaatc cggataaaaa gaaactggcg gcgaaaggca ttacttcagt gcgttcccgc
    36721 gtgaccaacc tcaccgagct gctgccgggg ctcacccatg agcaggtttg cgaggccata
    36781 accgaggcct ttttcgccca ttatggcgag cacgtggaag cggaaatcat ctccccggac
    36841 aaaacgccag acttgccaaa cttcgccgaa acctttgccc gtcagagtag ctgagagtgg
    36901 aacttcggtc aggctccggc attctcgcat ctgctggatg aacgctttag ctggggcggc
    36961 gtggaactgc atttcgacgt tgaaaaaggc catatcaccc gcgcccaggt atttaccgac
    37021 agcctcaacc ccgcgccgct ggaagccctc gccgggcgac tgcaaggctg cctgtaccgc
    37081 gcggatatgc tgcaacaaga gtgcgaagcg ctgttggttg acttcccgga ccaggaaaaa
    37141 gagctacggg agttgtcgac gtggatagcg ggggcggtaa ggtaactcgc tgattaaaat
    37201 atccaaggaa ggaaatatga tcgatctgga aaaccaggaa cgggagatta tcaatttaat
    37261 gttcagccag cgtatttcct ggctagccgc cgtgcgtatc aggcataaat tatcgcttgc
    37321 tgaagtaagt aaaatgttag gtatctcgat taattctcta aaacagatcg aaaagacgga
    37381 acggcttagc tcaaacatca aaagcaaaat ggcagaaatt tatggttgtc caccagagtt
    37441 gctgatatgc ccttcctgga tgacggctga acataaatga atagattcgt gaatagatac
    37501 ttgaatgtat tttattttct atccatatga aaaataatag taaatctata aatcagttgc
    37561 tattgatgaa tagatactaa aaagccggag cagtccccca ctccggctta acacgacaat
    37621 cgcctttcag cgaaacacaa ttactcttta tcgcccagca gaacggattc cagtgcgatt
    37681 ttgatcatgt cgttgaaggt ggtctgacgc tcagcggcag tggtctgctc gtgagtgcgg
    37741 atgtggtcag acacggtgca gatggtcagg gctttcgcgc caaactctgc agccacgccg
    37801 tagataccag ccgcttccat ttccacgccg aggatgccgt atttttccat cacgtcgaac
    37861 atttcgccgt ccggagagta gaacaggtca gcggagaaca ggttacccac gcgagcatca
    37921 acgcccagtg ctttagctgc atctactgcg ttacgcacca tgtcaaagtc agcgatagcg
    37981 gcaaagtcat ggtctttaaa acggatgcgg ttaactttgg aatcggtgca ggcccccata
    38041 ccgataacga cgtcgcgcag ttttacgtgc ggcagaactg cgccacagga accaacgcgg
    38101 ataattttct tcacgccgaa atcggtgatc agttctttgg tgtagatgga gcaggacggg
    38161 atacccatac cgtgacccat tacggaaatt ttgcggcctt tgtaagtacc agtgaagccc
    38221 agcataccgc gaacgttgtt cacttcacgg gcatcttcaa ggaaagtttc agcaatatac
    38281 ttcgcacgca gcgggtcgcc tggcatcaaa actacgtcag cgaaatcgcc catttctgca
    38341 ttaatgtgtg gggtagccat tgttttatcc tttttgtgac ataacaaagg cggagtcaac
    38401 gctccgccca aatccatcag aacatggctt tgccatattc catatcagaa gtaccaaaat
    38461 attttgccag agtctggccg atatccgcga aggtttcacg gtgacccagt gagcccggtt
    38521 ttactttcgg gccatatacc agtaccggaa tgtgttcacg cgtgtggtca gtaccggtcc
    38581 aggtcggatc gcaaccgtgg tcagcggtga ggatcaggat gtcgtcatcg cgcagcagag
    38641 acatcagctc cggcagacgg cggtcgaaca gttccagacc cgcagcataa ccggcgacgt
    38701 cgcgacggtg gccccaggaa gagtcgaagt caacgaagtt ggtgaagacg atggtgttat
    38761 cacccgcttc tttcatctct ttgatggtgg cgtcaaacag cgcgtccaag ccagtcgctt
    38821 tcactttttt ggtgataccg cagttggcgt agatgtctgc aattttaccg acagaaacca
    38881 cctggccgtg tttttcatca accagtttct gcagcacggt cggtgctggc ggctcaacag
    38941 ccaggtcgtg acggttaccg gtacgctgga agttaccagc tttgtcaccg ataaacggac
    39001 gagcgataac acgaccgata ttgtagccgc cgttggtcag ctcttcacgg gcgatttcgc
    39061 acagttcgta gagtttatcc agaccgaaag tttcttcatg gcaggcaatc tggaacacgg
    39121 agtcagcgga ggtatagaaa atcggcttgc cggttttcat gtgctcttcg cccagttgat
    39181 ccagaatgac cgtaccggaa gagtggcagt taccgaggta acccggcaga ttagcgcgtt
    39241 cgaccagttt atccagcagc tcttgcggga agctgttttc gtgatcggag aaatatcccc
    39301 actcaaacag aaccgggaca ccggcgattt cccagtgacc agacggggta tctttaccgg
    39361 atgacatttc gtgcgcccat gcgtacgcgc cgataacttc agcgttgccg tccattcccg
    39421 ccggaatgaa accggtagaa ccttcgtgtg ctttcgccag ccccagacgg atcagatttg
    39481 gcagattgag cggcccttta cgaccattat cagcttcgcc tttggcacaa gcttctgcga
    39541 tatgacccag ggtgtcagcc ccgacgtcac caaagcgttc tgcatcttct gtagcgccga
    39601 tgccgaatga gtccagcacc ataataaatg cacgtttcat atgttctccg tactcagtgc
    39661 ttcaaattta aatgatcaga tcagtatacc gttattcgct gatacggcga tagacagttg
    39721 gtgtgctttc cggtgcgtta tcggcaagtt taattgccgc tttcaccgct ttcgccgctt
    39781 cctgccagct gttttcgtct ttcgcgagga taaccgccag cggacgctga ccgtctacct
    39841 ggtcgcccag acgcgccata tcagtaaagc cgacgctgta atcgatggtg tcagatgcct
    39901 gacgacgacc gccgcccatt gcaaccactg ccatccccag cgcgcgggta tccatttcac
    39961 tgacaaaacc ttcggtatca gcatagactg ctttcgtcag catcgctgtc ggcagatact
    40021 tcgcgtagtt ctcaacgaag tcggtcgggc ctttttgtgc cgctaccata cgaccaaaga
    40081 cttctgccgc tttaccgttg tccagcaccg cctgcaattt cgcgcgcgct tcggcgtcat
    40141 ctttcgccag tttgccggag atcagcatct ccacgcacag caccatcgtg acatcaaaca
    40201 gacgcgggtt acgatactca cccgtcagga actgcaccgc ttcacgaact tcaaccgcgt
    40261 tacctgcact ggaggccagt acctgattca tgtcggtgag cagcgcggtg gtgcgcacgc
    40321 cagcgccgtt agccacgcca acaatcgctt cggcaagggc ttcagagagt tcgtaggtcg
    40381 gcataaacgc gccgctaccc actttcacgt ccatcaccag cgcgtccaga ccttccgcca
    40441 gtttcttcgc caggatcgag gcagtgatca gcgggatgga gtccacggtt gcggtaatat
    40501 cacgggtcgc gtagaaacgt ttatcagccg gagccagtga actggtctga ccgataatcg
    40561 ccacgccgac gtctttaata atttcgcgga aacggttgtc atccgggaaa atgtcgaagc
    40621 cagggatgga ttccagtttg tcgagcgtac cgccagtatg accgaggccg cgaccggaga
    40681 tcatcggaat atagccgccg caggctgcga ccatcggccc caacatcagc gaagtgacat
    40741 cgccgacgcc gccggtcgag tgtttatcaa caatcgggcc atccagatgc aggcttttcc
    40801 agtcgagaac ggttcctgaa tctcgcatcg ccatggtcag cgagacacgc tcaggcattg
    40861 tcatatcgtg gaagaaaatg gtcatcgcga gggcggcaat ctgcccttcg gaaatagtgt
    40921 tgtcgcgaat accgttgata aagaaacgaa tttcttcatc gctcagcgca tgaccatcac
    40981 gttttttacg aataatttct tgtgcgagaa acaaggtaac ctcccgagga aaagagtatg
    41041 aggcggcttt tgccggatgc gtctacgcct tatccggcct acgggtagat acgtctcgta
    41101 ggcctgataa gcgcagcgca tcaggcgtaa agcatcttac ttagtagctg ctggcgctct
    41161 taccgtcgcc gtggcccagc gctttcagca ggcttgccag caggctggaa gcaccaaagc
    41221 ggtagtgacg cgcatcagcc cagtcagcac cgaacagttc atctgcgatg gcgagatatt
    41281 tctgcgcatc ttccgcagta cgcacgccgc ccgccggttt gaaaccaacg gttttttcta
    41341 cgcccatatc acggatcact tccatcatga tgcgagcgct ttccggcgtc gcgttcacag
    41401 ccactttacc ggtagaggtt ttgatgaagt ccgcacccgc tttgatggag atttcagacg
    41461 ctttacggat cagcgcttcg tctttcagtt cgccggtttc gatgatcact ttcagcagta
    41521 cattcgctgc cgcgcaagcc tctttacagg ctttcaccag gtcaaaacca acctgctcgt
    41581 cacccgccat cagcgcgcgg tacgggaata ccacgtcaac ttcatcagca ccgtaggcga
    41641 ttgccgcacg ggtttctgcc agcgcgattt cgatgtcgtc gttaccgtgt gggaagttgg
    41701 ttaccgtagc gatacggatt tccggggtgc cctgctcttt cagagttttg cgagcaatcg
    41761 ggataaaacg aggatagata cagatagcgg cggtattgcc gaccggagtt ttggcctgat
    41821 gacacagagc aattactttc tcgtcggtgt cgtcgtcatt cagggtggtc aggtccatca
    41881 atttcagtgc acgcaggctg cttgctttca gatcagtcat ttcattctcc tggcttgtcg
    41941 ccaataaaat tcaccttgcg agtttgttag tattctaaca tctactccgc aacacacttc
    42001 gatacacatc acaattaagg aaatctactt acaagtttgc atcactgtaa tgcgatctgg
    42061 ttcaaataat tcactttcaa atgaatgcgt cagtggtggc aaacgcatca ggatcaaagt
    42121 gaacatcacg aaacttctta caatggcgca tcaccttcgg cataaaagga aaagaagatg
    42181 cccacttctc atgaaaatgc actgcaacaa cgttgccagc aaattgtcac cagcccagtg
    42241 cttagcccgg agcagaagcg ccattttctg gcactggaag cagaaaacaa tctgccttac
    42301 ccacagcttc ctgccgaagc ccgccgcgcg ctggatgaag gtgtaatctg cgatatgttt
    42361 gaaggtcatg cgccgtacaa accgcgctat gtcttacccg attacgctcg ttttctggcg
    42421 aacggttccg aatggctgga gctggaaggc gcgaaagatc ttgatgacgc actctctctg
    42481 ctgaccattc tttaccacca cgtaccgtcg gtcacatcga tgccggtcta cctggggcaa
    42541 ctggatgcgt tgttgcaacc gtatgttaga attctaacac aaaacgagat cgatgttcga
    42601 ataaaacgtt tctggcgtta cctcgacaga accctgccag atgcctttat gcacgccaat
    42661 atcggcccgt ctgattcgcc cattacccgc gcgatcttac gtgcagacgc gcaggtttca
    42721 ccgaacctga cctttatcta cgatcctgaa atcacccctg atgacctgct gctggaagtg
    42781 gcgaagaaca tctgtgaatg tagcaaaccg cacatcgcca acggtccggt gcatgataaa
    42841 attttcacaa aagggggcta cgggattgtg agctgttaca actcactgcc gctggcgggt
    42901 ggtggcagca cgctggtacg ccttaacctg aaagccattg ccgagcgcag cgaatcgctg
    42961 gatgacttct ttacgcgcac tctaccgcac tactgccagc agcagatcgc catcatcgat
    43021 acgcggtgtg aattcctcta tcaacaatca cacttctttg agaatagctt cctggtgaaa
    43081 gaagggctga ttaaccctga acgttttgtg ccaatgtttg gcatgtatgg gctggcggaa
    43141 gcggttaact tgctgtgtga gaaagaaggg attgccgcgc gctacggtaa agaagccgcc
    43201 gcaaatgaag tgggttatcg catcagcgcg caactggcgg agtttgtcgc caataccccc
    43261 gtgaaatatg gctggcaaaa acgcgccatg ttacacgcac agtcggggat cagttccgat
    43321 atcggcacca cgccgggcgc gcgtttgccg tatggcgatg agccagatcc gatcacccat
    43381 ctgcaaactg tcgccccgca tcatgcttat tattattccg gcatcagcga cattctgacg
    43441 ctcgacgaaa ccatcaaacg taacccgcag gcactggtac agctttgcct cggtgccttt
    43501 aaagccggaa tgcgtgaatt taccgccaat gtcagcggta acgatctggt tcgcgttacc
    43561 ggttatatgg tgcgtttgtc ggatttagaa aaatatcgcg ccgaaggttc acgcaccaac
    43621 accacctggc tgggcgaaga agccgcacgc aacactcgta ttctggaacg ccagccgcgc
    43681 gtgataagcc atgaacagca gatgcgcttt agtcagtaag attatcccct tctcctgcgt
    43741 tgacgggcca ggcagtcgtc tggctctgtt tttgcagggc tgcaatctgc gctgcaaaaa
    43801 ctgtcacaat ccgtggacga tgggacgttg caatgactgt ggggagtgcg tgccacagtg
    43861 tccgcatcag gcgttgcaga ttgttgacgg caaagtggtg tggaacgctg tggtctgcga
    43921 gcaatgtgat acctgcctga agatgtgtcc gcaacatgcc acaccaatgg cgcaatccat
    43981 gagcgtggac gaagtgctta gccatgtgcg caaagcggtg ctgtttattg aagggataac
    44041 ggtaagcggc ggcgaagtca cgacccaact gccgtttgta gtggcgctgt ttactgctat
    44101 caaaaacgat ccgcaactgc gccatctcac ctgtctggtg gacagtaacg gtatgttgag
    44161 cgaaaccggc tgggaaaaat tactcccggt gtgtgacggc gcaatgctcg atctcaaagc
    44221 atggggtagc gaatgtcatc aacaattaac cggacgcgat aatcaacaga ttaagcgcag
    44281 catctatttg ctggcagagc gcggcaagct ggcggaactg cgtttgctga tgatcccagg
    44341 ccaggtggat tatttgcaac acatcgaaga actggcggcg tttatcaagg gacttggaga
    44401 tgttccggtt cgcctgaacg cgtttcatgc ccacggcgtg tatggcgagg cgcaaagctg
    44461 ggcgagcgcc acgccggaag acgttgagcc gttggctgat gcattaaagg tgcgcggggt
    44521 gagccggttg atatttccgg cgctctattt gtgacatgcc ggatacggcc tacggttcgg
    44581 cacagacttg taggcctgat aagacgcgtc aagcgtcgca tcaggcattg tgcgccaact
    44641 gccggatgcg gcgtgaacgc cttatccggc ctacggttcg gcacaaacct gtaggcctga
    44701 taagacgcgt taagcgtcgc atcaggcatt atgcgccgac tgccggatgc tgcctacgaa
    44761 gggcaaacta cccacgaacg ttaaacaccg catacgtgtt attaagcaac acttcggcaa
    44821 tctcatccgc gggttccggg cgtaactcgc aaagcacggc aaacacacgg gcagcctgtt
    44881 ccgggcggtt aggctgcccc tgaaaaccgt tgagcggcat atccggcgca tcggtttcca
    44941 gcaacaacga tgccagcggt aattttgcga tgacatcgcg ggttttactg gcgcgtggat
    45001 aggtgatagt accgcctacg ccgattttgt agcccagctg tacaaaccgt tcagcctgtt
    45061 gctggctgcc agaaaatccg tggacaacac cagcacgcgg caaatcgtgg cgtttaagat
    45121 gcatcgccag tttgtcgtgc gtgcgccgtg aatgcaggat caccggcaga tcgtagcgtt
    45181 tcgccagttt cagttgttcg tcgagtaacc actgctgcct ctcaaattgc ggatcgtcgc
    45241 cgaagagatc cagaccgatc tcccccaccg ccaccacctt cgccggacgc ctttccagcg
    45301 cctgctgtag ctgctcaaga gacacatcgc tatgtttttc caacatacca ggatgcaagc
    45361 ccaatgcggc atacagcggt tgataatttt ccgctaacgc ctgcacgcgg gcaaaatttt
    45421 ccgcctctgt cgccggaaca atgatcttgc ctacgcccgc ttgtgccgcg cgttgcaggc
    45481 tggcctcttc atcgccaata aaaggcggga aatcaaaatg gcagtgagtg tcgataaaac
    45541 ggcaaatcaa gccagatcct cattgttaaa tgtcgggtcg ttcgcctgcg gagcgtcgct
    45601 cacttcggca accagcgtat cgttggcgac tggcgcaggc ggaatgacaa tcgattccgg
    45661 cgtcaccact ggcaccagat ggcgggtaag cggcgctttt tcagttaata gcttgccaac
    45721 cgtggcgagg aaataacgac cgcataaacg cccaagttta tagtcttcac gcagcgccgg
    45781 aatccgactg ccaagcgcga tactatgtaa tggcttcggc ggataaattt cggatatccg
    45841 cagcttgccc ggtggtttct caataaattg ctgaatgtca cgatagctgg tttcatgatg
    45901 ctgcaccaga ttgaccagcg gctgcaggct actgtcaccc agccagcgtt ccatgcgttt
    45961 gaaccactgc ggcgtgtagt acatttgtga cggcacagtg cggatgacga ccaacgtttt
    46021 agcgccctgc cttgccgcct ctttaaccgg aatcgcatca ctgatcccgc catccaggta
    46081 gttaatgcct tccagcgaca ctccgctacg ataaaagcca ggtatcgcac tggaggcgcg
    46141 aatcacatcc agccagtttt gtttggttgg taaaaagtaa ttcggcgcgt agtcatcctg
    46201 acgacaggcg cacatataaa acgatttgcc gctgtcaaac aaccgcgcgg cggtatccat
    46261 ttgcaacggc atctggcttg cggtggcctc caccagccag tcgagatcga taagatttcc
    46321 tccacggaca aagcgcaatg gatcaaaaaa ttcgcgtttt gtggtatagc gcatgatgac
    46381 tttgcgcgcg taaccgggct gattgcagat aaacgccgag aggttctgcg ccccggcaga
    46441 tgtgccgaga taaagatcga aaggattaaa ctgcgcgcgc ataaactcat ccagcacgcc
    46501 agccgtgaaa attccacgct gtccgccgcc ttcgcacacc agagctattc gtccaggctg
    46561 gaacggcctt agcgacaacg gcgcaatatt accaagcgtt acaggtattc tctgccccac
    46621 ttctgccttc ctgtttttgt tgtgaaagaa agacaaagta acgcaattta ttattcgcta
    46681 aaaccgcaaa agccagtccg ctggactggc tttggataaa attttcttaa tggtctgaaa
    46741 tctagggtcg ttttcggccc atgaacaaac tcactaggaa cagaataatc ccgacgacaa
    46801 agacaatttt agctgcgcct gcagcggtac cggccagacc accaaaccca agtgcggcgg
    46861 cgattaacgc gataaccaga aatatgatgc cccaacgaaa cataagcgtc tcctttacca
    46921 tagttaatgt caccgcttga tatgagcgtg aaaatcactc aatgcgatat ttttagtgtg
    46981 gtgcacatcg cgcctcccga caaagttcgg gaggacgaat tacgacgaat tacttagttt
    47041 tcagatcatt tttaacgctt ttcacaccat ctaccgcttt ggcgatactt tcagcacggt
    47101 cactttgtgc ctgagaatct acggtaccgg agagctgaac cacgccatcg gtggtttcaa
    47161 ctttcacttt acgtgaaggg acgatatcgt ccgccagcag tttggctttg atttcactgg
    47221 tggtggcggt gtcacccgcg tagtccttca ccgagccttc tttagcgtcg cgaacgtgca
    47281 gtttgtcgct gacagaggtc acgccttcaa cgcctttcgc cactttcact gcctcttcgg
    47341 cctgggcctg actttcaacg aaaccgctca gggtcacgac tttttgatcg gttttgacag
    47401 agatatcggt gctcttgatg ttgtcatgat ccaccagcgc cgccttcact ttcgcggtga
    47461 tggcgctgtc atccatgaaa ttaccgactt tattcataga gctatcgact ttttgccctg
    47521 cgctttcatt ggtagtctgc gcgttgtttt ccgcgtaggc agagccggtc gcgacggcag
    47581 aggtcaacat tacagccagc agagttttcg aaatcttcag tcttgtcata gtcatcgatt
    47641 tattcctgta tgtttgctcg taatttgagc ctagcaacac gaggttgcat tgctgaatag
    47701 ggagagactt caccctctac agaagtcaat ggtcgccatc acaaaagcga tgggtgatga
    47761 ataacgacca ttacagtctc tgaatcagtt attaatatcg gtagaatgac aatcgacggc
    47821 tttagatact gatatctacg cattgaacgg tatttaacgc catcagaaat gtcatcactt
    47881 tgttaaatat agatcacaat tttgaaaccg ctcgggatat cagcgaaaac ataagcaaaa
    47941 gtgaatgttt taagaacatt ccgtaagcgg ctaataagga agggaaattg acagggcgca
    48001 gcggctaccg cgccctggag gcaagaatta atgctcgcgg gtctggtgga actgaacgtc
    48061 cggataacgt tcctgtgcca ggcgcaggtt gaccatgctg gtagcgatgt aagcgaggtt
    48121 atcgccgcca tcaagcgcca gttggctttc gttcttacgc ttgaactctt cgaatttctt
    48181 cgcgtctgca cattctaccc agcgggcagt ggcaacgttg actgactcat acactgcttc
    48241 aacgttgtat tcgctcttca ggcgcgctac caccacatca aactgcagca caccaaccgc
    48301 accaacgatc agatcgttgt tggagattgg acggaacacc tgcaccgcgc cctcttcgga
    48361 aagctgtacc agccctttga gcagctgttt ttgcttcagc ggatctttca ggcggatacg
    48421 acggaacagt tctggtgcga agttcggaat accggtgaac ttcatcatct caccctgggt
    48481 aaaggtgtcg ccgatctgaa tggtgccgtg gttgtgcagg ccgaggatat cgcccggata
    48541 cgcttcttca acgtgcgaac ggtcacccgc cataaaggtc agcgcgtcgg agatcaccac
    48601 atctttcgca gtgcgcacct ggcgcagttt catgcctttt tcatatttac cggacaccac
    48661 acgcataaac gccacgcggt cgcggtgttt cgggtccatg ttggcctgaa ttttaaatac
    48721 gaagccggta aatttatctt cgctcgcttc tacggtacgg gtatcggtct gacgcggcat
    48781 cggcgcaggc gcccactcca ccaggccatt cagcatatga tcaacgccga agttacccag
    48841 cgcagtaccg aagaataccg gagtgatttc gcccgcaagg aacagctctt tgtcgaactc
    48901 gttagacgcg cctttaacca gttccagttc gtcacgcagc tgctgtgcca gatcttcacc
    48961 aaccgcagca tcgagatccg ggttattcag ccctttaaca atgcggactt cctgaatggt
    49021 gtgcccttta ccgctctgat agagataggt ttcatcttta taaaggtggt aaacgccttt
    49081 aaacagcttg ccgcagccaa ttggccaggt gatcggtgcg cagccaattt tcagctcgtt
    49141 ctcaacttca tcgagcagtt ccatcgggtc gcggatatca cggtcaagtt tgttcataaa
    49201 ggtgaggatc ggcgtgtcgc gcagacgggt aacttccatc agcttacggg tacgatcttc
    49261 aacacctttt gcggcgtcga taaccatcag gcagcagtcc accgccgtca gggtacgata
    49321 ggtatcttcc gagaagtctt cgtgccccgg ggtgtcgagc aggttaacca ggcaatcgtg
    49381 atacggaaac tgcatcacag acgtagtaat ggagatccca cgctgctttt ccatctccat
    49441 ccagtccgac ttagcgtgct ggttggaacc acggcctttt actgtaccgg cggtctgaat
    49501 ggcctgtccg aacagcagca ccttctcggt gatggtagtt ttaccggcgt ccgggtgaga
    49561 aataatggca aaagtgcggc gcttcgccac ctcttgcaaa taaggagaca acgtcataat
    49621 tctttcttct tgagtaagcg gcatcgcgcc gcgcttgttg aaaacgaaaa attgcggcta
    49681 ttttacccat caacgggggg gaggcaatca gtgtttacac aggagctgct ccagttcgtg
    49741 caacgaagaa acggtccagg tgggcgcgat gccttctggt tgctcgcgat tgtgcgcatt
    49801 cagccagcag gtcgcaagcc cggcgttgat gccaccgaga atatcggact cggcagtgtc
    49861 gccaaccatc agcacgcgtg aacggtcagg attgcccgcc tgttccagcg cataatcgaa
    49921 aattttctta ttcggtttgg caacgccaac ttcttcggaa atcaccagca aatcgaagta
    49981 atcacgcagg cccgtccgtt ccagacgcac ttgctgcaag gcgctaaaac cgttggtgat
    50041 gatgccgatt ttagcgttgc cacgaatggc gttaagcaga gaaaccgcgc ccggcaacgg
    50101 cgtacagatt tccgccatcg cattaataaa ggcatcgtta agcaagcctg cttcaacttt
    50161 cagccgttcg gcccagctct cgaaacgccc gtgctgaagc tgtaatgaag tgatcgcgcc
    50221 gttttgataa tccacccaca gtggcttgtt aacggcctga tagtcctgaa aatcttcagc
    50281 ggtaaaggtg acgctgtaat caagaaacat ccgctgcagg ccggtgaatg agtcaaaggt
    50341 aaacagcgtt tcatcggcat caaagaaaat ccagtcccac ttcattattc caccttgtct
    50401 tacatactga ttggcaacgc catgatgatg gcgtcttcgc gaccgtccgt ggtggggtag
    50461 taattgcggc gaatcgtcgc ctcgttaaag cctaaacttt cgtacagggc aatggcggca
    50521 gcatttgagg cacggacttc cagccatagt gtcgccacgc cgcgtttttc cagttcgtcg
    50581 atcagatgtt ccagcagcgc cctacccagc ccctgacgct gatagtcggg atcgaccgca
    50641 atattgaaca atgtagcttc atccagcacc acttgcgtaa tcgcaaacgc cgccattttg
    50701 ccgttttgcg ttaactgaaa gttgagataa cgctcgccct ggttgctggc aaaggttttt
    50761 tcactccacg gaaaggcgtg ggcgcgttgt tcaatgtggt aagccgctgg taaatcagtc
    50821 gtttcgaggg aagaaatcgt gttcatatgt gcaaatttgt tgccataacg cggcgcgtgc
    50881 cgttgggttt gcccgtaatt cggtgagcgc cggtgatgcc acctgagcgc cttccaatga
    50941 tagcggttcg tcagtaccca accgccagct gttgcagcga ctgccttgcg gcagcatcgc
    51001 gattttttct ggcgtcagtt gcagcacttg atcggggctg acggttaatg cgcgcagaac
    51061 atcgctcact aaaggatcag tcagggcggg aagatcgttt gccaccatca ccagacggac
    51121 gtgtgccggg ctcgcaatgg caatctcccc ctgcaacgcg ccagggcgac gcagcgacca
    51181 ctgggtaatg cccagttgct gtaactgcca gtctcgtcgg gatgtcatag cgaatcgctc
    51241 ctgttgtcag gggcgcaaat atagcaaatt cgtcgatacc gcgccaacat atggctataa
    51301 tcgccgccag tatcaattga ggagcattcc atgtctgcat ttaccccggc aagtgaagtc
    51361 ttgctgcgtc acagtgatga tttcgaacaa agccgtattc tgtttgccgg agacttacag
    51421 gatgacctgc ccgcgcgttt agataccgcg gccagccgtg ctcataccca gcaattccac
    51481 cattggcagg tgttaagctg ccagatgggg gataacgccc gttttagtct ggtcgccacg
    51541 gcgaatgatg tcgcagattg cgatacgctg atttactact ggccgaagaa caaaccggaa
    51601 gcccagttcc agttgatgaa tttactttct ctgctgccag tggggacaga tatttttgtc
    51661 gttggcgaga accgcagcgg cgtgcgcagc gctgagcaga tgctggcaga ttatgcgccg
    51721 ttgaataaag tcgacagcgc tcgtcgctgt ggcctctatt ttggtcgtct ggaaaaacag
    51781 ccagtatttg atgcggataa attctggggc gaatacagcg tcgatggcct gacggtcaaa
    51841 acgctgcctg gcgtgtttag ccgcgacggt ctggatgtcg gtagccagtt gctgctctcg
    51901 acgttaactc cgcacacgaa aggtaaagtg ctggatgtcg gctgtggcgc gggtgtactt
    51961 tcagttgcct ttgcgcgcca ttcgccgaaa attcgtctca ccttgtgcga tgtctctgcc
    52021 ccggcggtag aagccagccg cgcaacactt gcagccaacg gtgttgaagg tgaagtcttt
    52081 gccagcaacg tcttttccga ggtgaaaggt tgttttgata tgatcatctc caacccgccg
    52141 ttccacgatg ggatgcaaac cagcctggat gcggcgcaaa cgctgattcg tggcgcggtg
    52201 cgtcatctta atagcggcgg cgagctgcga attgtagcga acgccttcct gccttacccg
    52261 gacgtgctgg atgagacatt tggcttccat gaagttatca cgcaaaccgg gcgcttcaag
    52321 gtatatcgcg ccattatgac ccgccaggcg aagaaaggtt gagtatctga tatctgtcgc
    52381 cgcataccgt gtcggatgcg gcgtgaacgc cttatccgac ctacaaaacc tgcacgttag
    52441 cccttcgtag gccagataag acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct
    52501 accaaacctg cacgctagtt tcctgatgga catttttcca gcaattacac atcaggcgat
    52561 aattaactat tgacgaaaag ctgaaaacca ctagaatgcg cctccgtggt agcaattctt
    52621 tttaagaatt gatggtatgc gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt
    52681 agtgtcctta cggacgtggg ggttcaagtc ccccccttcg caccaaaaac cacgttgata
    52741 ttgctcgcac tgggcgaagg tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt
    52801 tcttacggac gtgggggttc aagtcccccc cctcgcacca aacgaggcga tatcaaaaaa
    52861 agtaagatga ctgtgcgaag gtggcggaat tggtagacgc gctagcttca ggtgttagtg
    52921 tccttacgga cgtgggggtt caagtccccc ccctcgcacc aattatcttt acttcctttc
    52981 ttgtttcttc cctgttttgt catccctgat tcaaattcat tatcatcagc atgatgccag
    53041 caataagcgc caggcacgat ggtagcaaaa tgtaatgccg caactgtttg tgatacagca
    53101 tcaccggcgt caatactaat cccatcagaa tgacaatccg ccactgatcc ggcgataaat
    53161 ccgcatacat cagccctgca atcagcaatc ccggcagcaa cactccccag ccactgccca
    53221 gcgtacgttg caacatggtt tcatctcttt cattgataat gaaaaccaat atcatatgat
    53281 aatttttatc atttgcaagc cagagaaatc ccttgctatc gagtaaacct atcgatagga
    53341 ttagcaatca ttatcattta gattactatc acgattatgg cctatcgttc cgcaccgctc
    53401 tatgaagatg tcatctggcg aacgcatctc cagccgcagg atgctgggct tgcacaagcc
    53461 gtacgggcga tgatcgccaa acatcgtgaa catttgctgg agtttatccg cctggatgaa
    53521 cccgctccgc ttaacgccat gacgctggcg caatggtcat caccgaatgc gctaagttct
    53581 ctactggcgg tctattccga tcatatctat cgcaaccaac caacgatgat ccgcgagaac
    53641 aaaccgctga tctcactatg ggcacaatgg tatatcggcc tgatggtgcc accattaatg
    53701 ctggcgctac tgacgcagga aaaagcatta gatgtgtcgc cggaacattt ccatgctgag
    53761 tttcacgaaa ccggacgcgt cgcctgtttc tgggtcgatg tgtgtgaaga taaaaacgca
    53821 acaccacatt cgccgcagca gcgaatggaa acgttaatca gccaggcgct ggttccggtt
    53881 gtgcaagcac tagaagcgac cggagagatc aacggcaaac ttatctggag taataccggt
    53941 tatttgatca actggtatct cactgagatg aagcaactgc tcggcgaggc tactgttgaa
    54001 tcgctgcgcc atgccctctt ttttgagaaa acgctcacga acggtgaaga taatccactc
    54061 tggcgtaccg tggtactgcg cgacggcctg ctggtgcgcc gcacttgctg ccagcgttat
    54121 cgcttaccgg atgtgcagca atgtggctat tgtacgctga aatgaaagca ctgccgggga
    54181 agtaaacccg gcatcatgcg gattaatagg gatgcaacac attacttgct tcgcaatgcc
    54241 gcatacataa cagaatatct gcggcctcca acaaccctgc atcagaactg acgcccagtt
    54301 tcgacatcac attaaattta tgcgctcgga tcgttttgat attacgttta agctgctcgg
    54361 caatttgtgt cattgagtag ccacgcgaca taaagcgcaa tatttctctc tccgtcgggc
    54421 ttaacgtccg gctttggtta atgtaccact gattgttcag tcggtcggtc gcctgacgta
    54481 caccatttaa tgacaaaaag aattcctgat gaaaaatctc cagcgttgac gctttactta
    54541 atacaccgtc cagcggtgat ggcgacaatg aaccaatcag ccgagcttca atatcatcat
    54601 ccacaataac taaacgccgg gtacgcggaa acttaatcgc cagctcagtc aggcaagata
    54661 atccctctct ccgctcactt ctcatggcag aaaaagaaaa aatgaccgcc gcaaacgaga
    54721 tccgcgacat ggcatcctga aatgactcct gactggtata ggtatggagc ttataagggt
    54781 tgcccgcaaa ctttctgaat aaactctcaa taccgatact actcatgacg catttttcta
    54841 tgagtgcgac atttctcttc ttaattcggc tgtgttccat tctgcggcat ctccataaac
    54901 agaaggcaag ttaagttctc ttgcaccctg cgcgctattt atccatgcat acatttcagc
    54961 gttgctgtga attgccagtc gacgcatcgc actgtttttt tgggcgctga tagttttatt
    55021 acttttctta agcaatgaag ctatctggtt gatgccccat cctttaccta atagccgcag
    55081 taccttgcgc tctgaaagag tgagaatgac ggagcgatag ctaaaatcat gagtttcagt
    55141 cattgccgga gaggtcagca tggcgctgat acgctctgca tgcgtattgc cggaacgtac
    55201 ggtcttgacc agattctcaa tgggttcaac atcagacaat aacgtcgccg tagggcacat
    55261 gagcagttct acagcctggg aataccagga gcgtgacacc atgaaaaccc agtgaatttc
    55321 ccgatattgt gagattaaag aataataatg ttcacaaaca gaacgtggat cttcactttc
    55381 accggctaaa t
//
LOCUS       DAJTXY010000014        53050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTXY010000014 DAJTXY010000000
VERSION     DAJTXY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.014"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(11..322)
                     /locus_tag="OO566_000945"
     CDS             complement(11..322)
                     /locus_tag="OO566_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6604328.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO566_000946"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO566_000946"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO566_000947"
     CDS             701..1054
                     /locus_tag="OO566_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6604329.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO566_000948"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO566_000948"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6604330.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLHQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OO566_000949"
     CDS             complement(2810..3280)
                     /locus_tag="OO566_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6604331.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO566_000950"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO566_000950"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6604332.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO566_000951"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO566_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6604333.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO566_000952"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO566_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6604334.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="OO566_000953"
     CDS             complement(5283..8432)
                     /locus_tag="OO566_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6604335.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO566_000954"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO566_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6604336.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="OO566_000955"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="OO566_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6604337.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="OO566_000956"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="OO566_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6604338.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO566_000957"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO566_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6604339.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO566_000958"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO566_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6604340.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="OO566_000959"
     CDS             14911..15288
                     /locus_tag="OO566_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6604341.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="OO566_000960"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="OO566_000960"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6604342.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="OO566_000961"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="OO566_000961"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6604343.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="OO566_000961"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="OO566_000962"
     CDS             18099..18428
                     /locus_tag="OO566_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6604344.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="OO566_000963"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="OO566_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6604345.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="OO566_000964"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="OO566_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6604346.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="OO566_000965"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="OO566_000965"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6604347.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="OO566_000966"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="OO566_000966"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6604348.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO566_000967"
                     /pseudo
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO566_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="OO566_000968"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="OO566_000968"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6604349.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="OO566_000969"
     CDS             complement(25481..27157)
                     /locus_tag="OO566_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6604350.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGKIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO566_000970"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO566_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6604351.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSQGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="OO566_000971"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="OO566_000971"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6604352.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO566_000972"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO566_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6604353.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="OO566_000973"
     CDS             complement(31205..31999)
                     /locus_tag="OO566_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6604354.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="OO566_000974"
     CDS             32137..32478
                     /locus_tag="OO566_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6604355.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO566_000975"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO566_000975"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6604356.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="OO566_000976"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="OO566_000976"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6604357.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="OO566_000977"
     CDS             36394..37686
                     /locus_tag="OO566_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6604358.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="OO566_000978"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="OO566_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6604359.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="OO566_000979"
     CDS             complement(38219..38677)
                     /locus_tag="OO566_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6604360.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="OO566_000980"
     CDS             complement(38674..39591)
                     /locus_tag="OO566_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6604361.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="OO566_000981"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="OO566_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6604362.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="OO566_000982"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="OO566_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6604363.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO566_000983"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO566_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6604364.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO566_000984"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO566_000984"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6604365.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO566_000985"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO566_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="OO566_000986"
     CDS             43550..44236
                     /locus_tag="OO566_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6604366.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46647
                     /locus_tag="OO566_000987"
     CDS             44233..46647
                     /locus_tag="OO566_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6604367.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47359..48453)
                     /gene="mnmH"
                     /locus_tag="OO566_000988"
     CDS             complement(47359..48453)
                     /gene="mnmH"
                     /locus_tag="OO566_000988"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6604368.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48522..49448)
                     /gene="allS"
                     /locus_tag="OO566_000989"
     CDS             complement(48522..49448)
                     /gene="allS"
                     /locus_tag="OO566_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6604369.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49678..50160
                     /gene="allA"
                     /locus_tag="OO566_000990"
     CDS             49678..50160
                     /gene="allA"
                     /locus_tag="OO566_000990"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6604370.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50238..51053
                     /gene="allR"
                     /locus_tag="OO566_000991"
     CDS             50238..51053
                     /gene="allR"
                     /locus_tag="OO566_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6604371.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51143..52924
                     /gene="gcl"
                     /locus_tag="OO566_000992"
     CDS             51143..52924
                     /gene="gcl"
                     /locus_tag="OO566_000992"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6604372.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALNSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52937..>53050
                     /locus_tag="OO566_000993"
     CDS             52937..>53050
                     /locus_tag="OO566_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6604373.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPY"
BASE COUNT        12778 a        13672 c        13969 g        12631 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggtgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccggg aagggcagga tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctggc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aatcagcgag aatttgctgg cgtaatgtgt gctcttccgc tagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagct ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcat tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc cagccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaaccg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gtttttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgtgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgctactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcatca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caatatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacta ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg cggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaagc
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacgg ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggcgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggcgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca ggcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgac atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaacc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagccg gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctc atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc gccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactcctccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagcaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa
    23461 taacaacaag cagccacaat ttcctctatc gcttgtggaa aacgcgcttc aggtgaaagg
    23521 gtgtaatcaa taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga
    23581 tcgtgggtat cgagattgcc gagaataaaa ccgccttcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaaaattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagat ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 cctggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga tagggtaaac agttcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gattttccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggccggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggatcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaatcatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa ggggccctgg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgccg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    28801 taacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acccggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gtgggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 actacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tactcactac ggtaatcgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tacggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgccgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgaccat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agaaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac aggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata accgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggcgatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaac
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatacg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tacaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct ctggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 ggtcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tatccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcgatctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cggacgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaacgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgagttcgc catgcttggg
    46441 tttgtttccg gcctggtggc cgcaattggt gcggaaacgg cactggcagt gttgcaggcg
    46501 aaagtgtttg atttcccgtg ggagccagac tggcgattgt ggattgttct gccgtgcagc
    46561 ggtgcgctgc tgctgtcgct tttcggcggc tggctgggtg cgcgactggt taagggtaag
    46621 gcgctgttca ggcagtttgt ggggtgatga caggtgttag attgcctgat gcgctacgct
    46681 tatcaggcct acgaagagcg tgcaatatat tgattttgca agattgtgta ggccggataa
    46741 ggcgtttacg ccgcatctgg catgaacagt gtgcattttg gcaataaata tgaaaccaac
    46801 tgtcatgtcg gtttttttaa aggccgaagg aggtaaacct tcggccttag ctacgtttcc
    46861 tcagttattc aataaactga cggatcagtt ccagcagcgc gattagcgcc ttaagaacga
    46921 tgagaacttt atcgagaaaa gttttcatcc gttgctccct taagggagaa agacaatcac
    46981 agcttaccct tacgttgcct gtaactatga ttttcttcat gtgctagatg aggactgcct
    47041 ctctccaaat tagagcacca gaatcagcac cacctgtatc ctgaccagga cttaaaagta
    47101 cttttccgca ctgcggtaat cccttgaggc agtatcacta ctagcgtcgg gattgtatcg
    47161 ccaacatagc tgtttgaata tgtaggcatc ctgacttata attcacttgc tagatgagga
    47221 ctgtttaatt tctgcccgga acaaccaagt cttgggcgga aaaaataaaa aaaccgactg
    47281 tcatgtcggt tttttatatc tgtattctgg tgcaaccatt ttgttctcac gacaatcgcc
    47341 gtttcagaat accgcccatc actgcgcctt aacccatacc gccacttccg cccactcacc
    47401 gcgaaagaca actttttccg cttttttctc aagctgatag cgatacattg ggtcgtaata
    47461 ttctttaagt aacggcacca gccaggccag atgaccgtcg gtgctgccgg tggtgagttg
    47521 cgttgtcagc gccgtatcca gctgggcagc gagttcgtta tagcgctgta accccagccg
    47581 acgcttaatc gccgaaagtc cgtgatgcag gtattcgcaa tattcctgcc agccctgttc
    47641 gtcgccataa gcgtgggtaa aatcatgatg catacgcaag aaatactctt cattcaggcg
    47701 ttcaagacgg atctcaaacg gatcttctac caccgcaatc gccgcctgag tcattcgctc
    47761 gcgcagacat tccggcaggt gattcgaacc gatcatccgg ctttcatctt ccagcaccca
    47821 caggcgcaaa tcctgacggg cgtcggtttt tagcatttcg gcagccagca ggttttcaaa
    47881 actcgcctgg ctaagttgtg gttgtaacgt gcgaccaaac gccgaaccgc gatgacgcgc
    47941 cagcccttcc agatcaacac cgtttggctg ttgttgcact aatagcgttt taccgctgcc
    48001 ggtacaaccg ccaatcagca ctatcggttt ttgtgccagt tcaatggtcg cctgaatcgc
    48061 ggtctggcgc agtgccttat aaccgccttc caccagcgga taatcaatcc ccgcttcatg
    48121 cagccagcgt tgcacaatat gtgaacgttg accgccacgg gcgcagcaga gaataccttg
    48181 cggattttgc aggcacgctg cccgccaggc gtacatacgc tgctgacgaa tttcacccgc
    48241 caccagttta tgccccagcg ccagcgctgc gtctgagcct tgctgtttat agcaggtgcc
    48301 aacggcggcg cgttcatcgt tattcattaa cggcagattg atagcggcgg gcattgcgcc
    48361 ttgctcaaac tcgatagggg cgcgaacatc aataatgggc gtatcagcaa tcagcagggc
    48421 acgatagtcc tgttccgtgt gtctctcttg catagttaaa agtgaacctc aaatcagctt
    48481 gcgcgctatt ttacgcgcca acgcgcaagg aaacttgatt tttaactgcg tgggttgccg
    48541 aaaatttcta aaaatccgct gatttccggc ctgtgttggg taaacaaggt cacaatatct
    48601 tctaccgctt tgccgctgcc aaatttgcgc catgccagac tcaatggcga aggagggcgc
    48661 atcgttggga ttacccggct gaccagttgt tgattatcga tcattgactg gcaaagtgat
    48721 tttggcaaaa aaccaatgcc aacacccgcc agatgggcgg cgattttcgt ttccatatcg
    48781 ggaacaataa tctctttttg ccccggcaat cgccaggcga cgcgtttggt taaggtgcgg
    48841 gcgctgtctt caatattgac cgccggaaag cgacgcaact gcgcttctgt tagcggctct
    48901 tcaacgttcg ccagcggatg atccgccgcc atgacaaatc gccattgcac cgatcctaag
    48961 ggatcaagac taaaggtatt tgccagcgcc tcagttcccg tgaccccgat agccagcgaa
    49021 aaaccttcgt acaatagcga gtcccagacg cccatataga tttgtcggga gatgtgaaac
    49081 tgggtaaagg ggtaacgctc attcagccac gccagcaact gggcgacggc ctgggggtta
    49141 tagagcaggt tgttgatgac aatattcacc tggcgttcca cgccatcatt cacctgttgc
    49201 agctcgctgg gcatactttc cagccagctc agccagtctc tggcctggga aagtagatgc
    49261 tcgccagccg ctgtcaacgt tacgctacga gtcgtacgga aaaacagcgc tactccggta
    49321 ttctcttcca gaagtttaat gcgataactg atcgtcgccg tggttttaca taatcgttct
    49381 gccgcttttg aaaaacttcc tgtttcagca accgcaatga aagtccgcaa ggtttctgga
    49441 tcgaacatct tcaggtatcc ccttttaaat ccgcaagttg cgtgattttc ttatcctctg
    49501 atttatcagt atttttacat gataatcctg ttcaatttgt ggaccaaatc tagttttgga
    49561 aaaacattcc aacttttgta ttgatgttgt tctcttaagg ttttagattg cctgttattg
    49621 aaaccaagct gaccggtcgg cggtggttga acggaattat gttacaagga caaaaagatg
    49681 aaacttcagg tattaccgtt aagtcaggaa gcctttagtg cttatggcga cgtaatcgaa
    49741 acgcagcaac gggatttttt ccatattaac aatggcctgg tggagcgtta ccacgatttg
    49801 gcgctggttg agattcttga gcaagaccgt acgcttatca gcattaaccg cgcgcaaccg
    49861 gcgaatctgc cgctgaccat tcacgaactc gaacgtcatc cgctgggcac tcaggccttt
    49921 atcccaatga aaggtgaagt gtttgtggtg gtcgtggcgt taggcgacga caaaccagac
    49981 ctgtcaacgc tgcgggcgtt tatcaccaac ggtgaacagg gggtgaatta ccatcgtaac
    50041 gtctggcatc acccactttt cgcctggcag cgcgtcaccg attttctgac catcgatcgc
    50101 ggcggcagtg acaactgtga tgttgaaagt attcctgaac aggaactctg ttttgcgtga
    50161 cgcctgcaac cgacttgcat aagataaact aattgttcat tgtttatgct cacttgtagg
    50221 tcggagttaa cgtaggtatg acggaagtta gacggcgcgg caggccagga caggcggagc
    50281 ctgtggcaca gaagggcgca caggcgttag agcggggaat tgcgattctg caatatctgg
    50341 aaaaaagtgg gggaagttcg tcggttagcg atatttctct caatctggat ttgccgctct
    50401 ccacgacctt tcgcttgctg aaggttttac aagcagcgga ttttgtctat caggacagtc
    50461 aattaggctg gtggcatata ggattaggtg tctttaacgt cggtgcggcg tacatccata
    50521 accgcgatgt cctctccgtc gccgggccgt ttatgcgccg cctgatgtta ctttccggcg
    50581 aaacggtcaa tgtcgcgatc cgtaacggca atgaagcggt attaattggt cagttagagt
    50641 gtaaatcgat ggtcaggatg tgtgcgccac tgggcagtcg tctgccgctg catgcttccg
    50701 gtgcgggcaa agcgctgctt tatccgctgg cggaagagga gttgatgagc atcattctgc
    50761 aaaccggttt gcagcagttt acgccaacta cgcttgtgga tatgcccacc ttgctgaagg
    50821 acctggaaca agcgcgtgaa ctgggctata ccgtagataa agaagagcat gttgtaggtc
    50881 tgaattgcat agcttcagca atttacgatg atgtcggtag cgttgttgcc gctatctcca
    50941 tctccgggcc ttcatcaaga ctgacagaag atcgttttgt cagtcagggt gagctggtca
    51001 gagacaccgc ccgcgatatc agcacggcgt tgggactgaa agcacatcca tagtgtctgt
    51061 cgcatcccgc tctgcggagc gggttttttt gacaaaattt aaaagttgga aaatttttcc
    51121 aattaataga ggtaggaata aaatggcaaa aatgagagcc gttgacgcgg caatgtatgt
    51181 gctggagaaa gaaggtatca ccaccgcctt cggcgttccg ggagctgcaa ttaatccgtt
    51241 ctactcagcg atgcgtaagc acggcggtat tcgtcacatt ctggcgcgtc atgtggaagg
    51301 tgtttcgcac atggcggaag gttatacccg cgcaacggca gggaatatcg gcgtatgtct
    51361 ggggacttcc ggtcctgcgg gcacggacat gatcaccgcg ctcaattccg cttctgctga
    51421 ttccattcct attctgtgca ttaccggcca ggcaccgcgc gcccgtctgc ataaagaaga
    51481 ttttcaggcc gtagatattg aagcaattgc taaaccggtc agcaaaatgg cggttacagt
    51541 tcgtgaagcg gcgctggtgc ctcgcgtgct gcaacaggca tttcacctga tgcgttctgg
    51601 tcgtccgggt ccggtactgg tggatttacc gttcgacgtt caggttgcgg aaatcgagtt
    51661 tgatcctgac atgtacgaac cgctgccggt ctacaaacct gctgccagcc gtatgcagat
    51721 cgaaaaagct gtagaaatgt taatccaggc cgaacgtccg gtgattgttg ccgggggcgg
    51781 ggtaatcaat gctgatgcag ctgcactgtt acaacagttt gctgaactga ccagcgttcc
    51841 ggtgatccca acgctgatgg gctggggctg tatcccggac gatcatgaac tgatggccgg
    51901 gatggtcggt ctgcaaaccg cgcatcgtta cggtaacgca acgttgctgg cgtccgacat
    51961 ggtgtttggt atcggtaacc gttttgctaa ccgtcatacc ggttcggtag agaaatacac
    52021 cgaagggcgc aaaatcgttc atattgatat tgagccgacg caaattggtc gcgtgctgtg
    52081 tccggatctc ggcattgtct ctgatgctaa agcggcgctg acactgctgg ttgaagtggc
    52141 gcaggagatg caaaaagcgg gtcgtctgcc gtgtcgtaaa gaatgggtcg ccgactgcca
    52201 gcagcgtaaa cgcactttgc tgcgcaaaac ccacttcgac aacgtgccgg tgaaaccgca
    52261 gcgcgtgtat gaagagatga acaaagcctt tggtcgcgat gtttgttatg tcaccactat
    52321 tggtctgtca caaatcgctg cggcacaaat gctgcatgtc tttaaagacc gccactggat
    52381 caactgtggt caggctggtc cgttaggctg gacgattccg gcggcactgg gggtttgtgc
    52441 cgctgatccg aaacgcaatg tggtggcgat ttctggcgac tttgacttcc agttcctgat
    52501 tgaagagtta gccgttggcg cgcagttcaa cattccgtac atccatgtgc tggtgaataa
    52561 cgcctatctc ggcctgattc gtcagtcaca acgcgctttt gacatggact actgcgtgca
    52621 actcgctttc gagaatatca actccagtga agtgaatggc tacggtgttg accacgtaaa
    52681 agtagcggaa ggtttaggtt gtaaagctat tcgggtcttc aaaccggaag atattgcgcc
    52741 agcctttgaa caggcgaaag ccttaatggc gcaatatcgg gtaccggtag tagtggaagt
    52801 tattctcgag cgtgtgacca atatttcgat gggcagcgaa ctggataacg tcatggaatt
    52861 tgaagatatc gccgataacg cagcggatgc accgactgaa acctgcttca tgcactatga
    52921 ataagggaga taaatgatgt tacgtttctc tgctaattta tcgatgttat ttggtgaata
    52981 tgattttctc gcccgttttg agaaagctgc gcagtgtggt tttcgcggcg ttgaatttat
    53041 gtttccttat
//
LOCUS       DAJTXY010000015        50328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTXY010000015 DAJTXY010000000
VERSION     DAJTXY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.015"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..372
                     /locus_tag="OO566_000994"
     CDS             <1..372
                     /locus_tag="OO566_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6604374.1"
                     /translation="IAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQIL
                     NAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
     gene            483..1406
                     /locus_tag="OO566_000995"
     CDS             483..1406
                     /locus_tag="OO566_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6604375.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1410..2228)
                     /gene="nlpA"
                     /locus_tag="OO566_000996"
     CDS             complement(1410..2228)
                     /gene="nlpA"
                     /locus_tag="OO566_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR6604376.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2450..2743
                     /locus_tag="OO566_000997"
     CDS             2450..2743
                     /locus_tag="OO566_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604377.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2784..3974)
                     /gene="nepI"
                     /locus_tag="OO566_000998"
     CDS             complement(2784..3974)
                     /gene="nepI"
                     /locus_tag="OO566_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6604378.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4185..4637)
                     /locus_tag="OO566_000999"
     CDS             complement(4185..4637)
                     /locus_tag="OO566_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6604379.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4690..6024)
                     /gene="adeQ"
                     /locus_tag="OO566_001000"
                     /gene_synonym="yicO"
     CDS             complement(4690..6024)
                     /gene="adeQ"
                     /locus_tag="OO566_001000"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6604380.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6199..7965
                     /gene="adeD"
                     /locus_tag="OO566_001001"
     CDS             6199..7965
                     /gene="adeD"
                     /locus_tag="OO566_001001"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6604381.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPAVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8011..9402)
                     /gene="uhpT"
                     /locus_tag="OO566_001002"
     CDS             complement(8011..9402)
                     /gene="uhpT"
                     /locus_tag="OO566_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6604382.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9540..10859)
                     /gene="uhpC"
                     /locus_tag="OO566_001003"
     CDS             complement(9540..10859)
                     /gene="uhpC"
                     /locus_tag="OO566_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6604383.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(10869..12371)
                     /gene="uhpB"
                     /locus_tag="OO566_001004"
     CDS             complement(10869..12371)
                     /gene="uhpB"
                     /locus_tag="OO566_001004"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6604384.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12371..12961)
                     /gene="uhpA"
                     /locus_tag="OO566_001005"
     CDS             complement(12371..12961)
                     /gene="uhpA"
                     /locus_tag="OO566_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6604385.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13036..13326)
                     /gene="ilvN"
                     /locus_tag="OO566_001006"
     CDS             complement(13036..13326)
                     /gene="ilvN"
                     /locus_tag="OO566_001006"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6604386.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13330..15018)
                     /gene="ilvB"
                     /locus_tag="OO566_001007"
     CDS             complement(13330..15018)
                     /gene="ilvB"
                     /locus_tag="OO566_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6604387.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(15124..15222)
                     /gene="ivbL"
                     /locus_tag="OO566_001008"
     CDS             complement(15124..15222)
                     /gene="ivbL"
                     /locus_tag="OO566_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6604388.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15787..15876
                     /gene="tisB"
                     /locus_tag="OO566_001009"
     CDS             15787..15876
                     /gene="tisB"
                     /locus_tag="OO566_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6604389.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16156..17340
                     /gene="emrD"
                     /locus_tag="OO566_001010"
     CDS             16156..17340
                     /gene="emrD"
                     /locus_tag="OO566_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6604390.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17348..17845)
                     /locus_tag="OO566_001011"
     CDS             complement(17348..17845)
                     /locus_tag="OO566_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6604391.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17842..18204)
                     /locus_tag="OO566_001012"
     CDS             complement(17842..18204)
                     /locus_tag="OO566_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6604392.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18194..18541)
                     /locus_tag="OO566_001013"
     CDS             complement(18194..18541)
                     /locus_tag="OO566_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6604393.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLTWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18650..19063
                     /locus_tag="OO566_001014"
     CDS             18650..19063
                     /locus_tag="OO566_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604394.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19110..20493)
                     /locus_tag="OO566_001015"
                     /pseudo
     CDS             complement(19110..20493)
                     /locus_tag="OO566_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(20528..22243)
                     /locus_tag="OO566_001016"
                     /pseudo
     CDS             complement(20528..22243)
                     /locus_tag="OO566_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22410..23303
                     /locus_tag="OO566_001017"
     CDS             22410..23303
                     /locus_tag="OO566_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6604395.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23300..24114)
                     /locus_tag="OO566_001018"
                     /pseudo
     CDS             complement(23300..24114)
                     /locus_tag="OO566_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24114..25730)
                     /locus_tag="OO566_001019"
                     /pseudo
     CDS             complement(24114..25730)
                     /locus_tag="OO566_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            26025..26699
                     /locus_tag="OO566_001020"
     CDS             26025..26699
                     /locus_tag="OO566_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6604396.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26738..28399)
                     /locus_tag="OO566_001021"
     CDS             complement(26738..28399)
                     /locus_tag="OO566_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6604397.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28595..29023)
                     /gene="ibpB"
                     /locus_tag="OO566_001022"
     CDS             complement(28595..29023)
                     /gene="ibpB"
                     /locus_tag="OO566_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6604398.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(29135..29548)
                     /gene="ibpA"
                     /locus_tag="OO566_001023"
     CDS             complement(29135..29548)
                     /gene="ibpA"
                     /locus_tag="OO566_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6604399.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(29542..29614)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            29853..30185
                     /locus_tag="OO566_001024"
     CDS             29853..30185
                     /locus_tag="OO566_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6604400.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGSKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(30187..31401)
                     /locus_tag="OO566_001025"
     CDS             complement(30187..31401)
                     /locus_tag="OO566_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6604401.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            31502..32520
                     /locus_tag="OO566_001026"
                     /pseudo
     CDS             31502..32520
                     /locus_tag="OO566_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(32481..33818)
                     /locus_tag="OO566_001027"
     CDS             complement(32481..33818)
                     /locus_tag="OO566_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6604402.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33893..35041)
                     /gene="dgoD"
                     /locus_tag="OO566_001028"
                     /pseudo
     CDS             complement(33893..35041)
                     /gene="dgoD"
                     /locus_tag="OO566_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(35038..35655)
                     /gene="dgoA"
                     /locus_tag="OO566_001029"
     CDS             complement(35038..35655)
                     /gene="dgoA"
                     /locus_tag="OO566_001029"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6604403.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKVVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35639..36517)
                     /gene="dgoK"
                     /locus_tag="OO566_001030"
     CDS             complement(35639..36517)
                     /gene="dgoK"
                     /locus_tag="OO566_001030"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6604404.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLLR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(36514..37203)
                     /gene="dgoR"
                     /locus_tag="OO566_001031"
     CDS             complement(36514..37203)
                     /gene="dgoR"
                     /locus_tag="OO566_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6604405.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37481..38137
                     /locus_tag="OO566_001032"
     CDS             37481..38137
                     /locus_tag="OO566_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604406.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(38183..38995)
                     /gene="yidA"
                     /locus_tag="OO566_001033"
     CDS             complement(38183..38995)
                     /gene="yidA"
                     /locus_tag="OO566_001033"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6604407.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(39109..39507)
                     /locus_tag="OO566_001034"
     CDS             complement(39109..39507)
                     /locus_tag="OO566_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6604408.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(39747..42161)
                     /gene="gyrB"
                     /locus_tag="OO566_001035"
     CDS             complement(39747..42161)
                     /gene="gyrB"
                     /locus_tag="OO566_001035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6604409.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(42190..43263)
                     /gene="recF"
                     /locus_tag="OO566_001036"
     CDS             complement(42190..43263)
                     /gene="recF"
                     /locus_tag="OO566_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6604410.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPRFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43263..44363)
                     /gene="dnaN"
                     /locus_tag="OO566_001037"
     CDS             complement(43263..44363)
                     /gene="dnaN"
                     /locus_tag="OO566_001037"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6604411.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44368..45771)
                     /gene="dnaA"
                     /locus_tag="OO566_001038"
     CDS             complement(44368..45771)
                     /gene="dnaA"
                     /locus_tag="OO566_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6604412.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            46068..46148
                     /locus_tag="OO566_001039"
     CDS             46068..46148
                     /locus_tag="OO566_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604413.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46378..46518
                     /gene="rpmH"
                     /locus_tag="OO566_001040"
     CDS             46378..46518
                     /gene="rpmH"
                     /locus_tag="OO566_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6604414.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46535..46894
                     /gene="rnpA"
                     /locus_tag="OO566_001041"
     CDS             46535..46894
                     /gene="rnpA"
                     /locus_tag="OO566_001041"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6604415.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCLLARGS"
     gene            46858..47115
                     /gene="yidD"
                     /locus_tag="OO566_001042"
     CDS             46858..47115
                     /gene="yidD"
                     /locus_tag="OO566_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6604416.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            47118..48764
                     /gene="yidC"
                     /locus_tag="OO566_001043"
     CDS             47118..48764
                     /gene="yidC"
                     /locus_tag="OO566_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6604417.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48870..50234
                     /gene="mnmE"
                     /locus_tag="OO566_001044"
     CDS             48870..50234
                     /gene="mnmE"
                     /locus_tag="OO566_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6604418.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        12422 a        13534 c        12688 g        11684 t
ORIGIN      
        1 atcgcaggct attacatgaa acgtattggc aagcgactat taatgctcat tgctatcgtg
       61 agtggtatgt gtttttacgc cagcgtactc atggcgacga ctccggcggt tgagctggaa
      121 ttgcaaattc taaatgccat cttccttggt attctctgtg gtatcggcat gctttatttt
      181 caggacttga tgcctgaaaa aataggctct gcgacaacgt tatatgcaaa tacttcacgc
      241 gtcggctgga ttatcgccgg ctctgttgac ggaattatgg ttgaaatctg gagctaccat
      301 gcgttgttct ggctggcgat agggatgttg ggtattgcga tgatttgcct gctgtttatt
      361 aaagatattt agctcaccag tctgggctaa gaaaaatcgg cgtggcgcaa acagagccat
      421 cctcgccatt tttgctatca tgcctgcata cataaacgac aaaacagtat gcagagggaa
      481 aaatgggttc caccagaaag gggatgctga acgttctgat tgccgccgtg ttgtggggaa
      541 gttcaggggt ctgtgcgcaa tacatcatgg agcaaagcca gatgtcgtcg cagtttttga
      601 ctatgacgcg tttgatattc gccggtttga ttctgctgac gctgtcattt gttcatggcg
      661 ataaaatctt ttctattatt aacaatcaca aagatgccat tagtctgctg attttttccg
      721 tggttggcgc gctaaccgta cagctcactt ttttgctaac catcgaaaaa tcgaacgcag
      781 ccacggcaac ggtgctgcaa ttcctctcac cgacgattat cgtcgcctgg ttctcactgg
      841 tacgtaaatc gcgcccgggc attctggttt tctgcgctat tttgacatcg ctggtcggga
      901 cttttttatt ggtgacacac ggtaatccga cgtcattatc gatctctcct gccgcgttat
      961 tctggggcat tgcctcggca tttgctgctg cattctatac cacctatccc tcaacgctga
     1021 ttgcccgcta tggcacgttg cctgtcgtcg gctggagtat gctgatcggc ggtctgattc
     1081 tattgccttt ttatgccaga caagggacaa actttgtcgt taacggcagt ttgattttgg
     1141 cgttttttta tttgatcgtc attggtacgt ccctgacatt tagtctgtac ctgaaaggag
     1201 cacaattaat tggcggtcca aaagccagca ttttgagctg tgcagaacca ttaagtagcg
     1261 cgctactctc tttgctgttg ctggggatca cgttcacatt accggactgg ctgggaacgc
     1321 tgctgattct gtcatcggta attttgattt caatggattc ccgtcgccgc gccagaaaaa
     1381 taaatcgtcc ggcgcgggat gagtgagaat taccagccag gcaccgcgcc accgttaaag
     1441 atggtttctg ccgctttagc gacttcggat gattgataag attgcagaaa ttccttcacg
     1501 ttttctgcat tcttattatc ttcccgtgcc accaaaatat tcacatacgg cgaattttta
     1561 tcttcaataa atacactgtc gtgcaccgga gaaagcccgg tctgctgaat gtaagtggtg
     1621 ctgataatcg ccacatcaac tttgggatca tccagtacgc gcgggagctg cgccccctcg
     1681 agttccataa tctgcaaatg acgaggatta tcggttatat ccagcgcggt aggtaataac
     1741 ccttttccct ctttcagagt aattagcttc tctttttgca acagtaaaag cgcacgacca
     1801 aggttggtcg ggtcgttggg gatcgccact gtcgcgcctt cttttatttg cgccacggtt
     1861 ttgatttttt tggaataacc cgccatgggg aagacaaacg tatttcccac cgctaccagt
     1921 ttataaccat gcgcctgatt atcttgttca agaaagggtc gatgctggaa gacgttcgca
     1981 tcaagttcgc catgatttgt cgcatcgttc ggtagcaacg aaccgctaaa tcctaccagt
     2041 tgaacatcga ggccatattt ctcttttgcc acctttttag cgacttccgc gacatcttgt
     2101 tctgcgccat ttataacgcc aactttaatg tgttttgcat cgctgctact ctggtcgcaa
     2161 cctgccagca gaattccggc cagcaataat gcggcccctg tccgtagatg atgtgttgtc
     2221 agtttcattt ttttatcctt ttattaatga ttacctgact atagcggtaa ggtcgctgcg
     2281 gtttaaaaaa cgaaacgcta tcgataagaa taaaaaggaa taaaagtgga atataaagtc
     2341 tggcacttct tactgaccac gcaagcgcgt ttcgtacagc atgatgagag cgatgaaagc
     2401 aaactacact tatgcttcat tcgatacaca tttgtaaaag gttgaatcca tgaaaccaac
     2461 gacactactg cttattttca cttttttcgc tatgccaggc atcgtatacg ccgagtcgcc
     2521 ttttagctca ctgcagtccg caaaagagaa aaccacggta ttacaagatt tacgtaaaat
     2581 ttgcacgcca caggcgtcat tatcagatga agcgtgggaa aagttaatgt tgtcagatga
     2641 aaacaataaa caacatatac gcgaggccat cgtggcgatg gaacgtaata atcagagcaa
     2701 ttattgggaa gcgttgggta aggtagaatg cccggatatg taagaacagc aacaactact
     2761 gaccaacgct tctttacctg atatcaggat ttcttcattt tcacctttgc agtaaccaac
     2821 aatgcagtca gcaacatcaa tgtgccggac aacatcaacg gcgaagtcag accaatatta
     2881 tccagcgcat aaccgccgat tgccgcgcca caggtattag caagctgaat aaccgccacc
     2941 tgaatagacc cggctttttc tgcctgatcg gccagcgagc gggtgatcca cgttgaccag
     3001 ccgacgggaa ccaatgcaaa agttagcccc cagataatcg ccacgccggt agcaacgatt
     3061 ttatcgcttc cccacaacgt cagtaccaac gcactcacag ccagtattaa cggcgcgcct
     3121 gctaaggcca gttttaccga acgtttaaga atgaacgacg aaagcgacgt accaataaag
     3181 ctggcgatac caaaactcaa cagcaccagc gttaagccat ccacgccgaa tcccgccagg
     3241 ttcatataca ccgggcgaat atacgtgaag aaagcaaact gcccggcgaa agacatgaag
     3301 atggcgatca tccctgccat cacacccgga cgttgtaata agcggaaagt attttgtttc
     3361 tgatgcgagg gttcgcctgg cagtgaaggc aatgatttga taatccagaa aatacacagc
     3421 acgcccatca ccgccgccgc attaaagaca ttgcgccaac cgataagctc gcctaaaaaa
     3481 ctgcccaacg gcgcggcaat caccagcgca atagaaaccg cgccgaagat caccgacagc
     3541 gccttcggca ccgtacgcgg cggtaccaga cgcatggtca gcgacgccga catcgcccag
     3601 aacccgccca gcgccagccc cagacaggca cgaccgatta aaagcaaact gaatgagtta
     3661 gcaaaggaaa ccagcaagca ggagagcgtc agcaaaacgg caaacaaaat aacaacgtag
     3721 cggcggtcag tggcctgaat tgtctgggta ataaacaaac tggcaaacat tgccacaaag
     3781 gcggtcacgg tcaccgattg cccggcaacc ccttccgaaa tgcctaaatc ctgggccatt
     3841 ggcgtcaaca aactgacggg caaaaactca acgataatca gacaggcgac acaaaacgcc
     3901 accgagaaaa cggctgacca gttcggtcgg gtgatggcat ccgcgccgcg gttttcggca
     3961 ataaattcac tcatggtgtt acccgtggca tgtttcaggg gaaaagccga ccactttaac
     4021 attgaaatgt gtgacgcatt taacgttttt gcaactttta cggtgttgct tgagctggag
     4081 ttaagatcga aacggaacag ggcaaagatt accggatgcg gcgtgaacgt cttatccacc
     4141 cgacaggtca ggcgcggtct gtaggctgat aagacgaaac ggtttcaatg caaccagtga
     4201 acgccctcgg caggctgaaa gaacgcgtta tagcgcatct gaaaagcatt aatatcctgc
     4261 atttcaggtt cggtttcacg cagaaaaccc agcgccagcc tgacctgagc atcggtaatc
     4321 ggcgcggaaa ggcgggcttt ttgtaaaatc cggtgccatt gcagcaggtt ctgttcgcta
     4381 tcatcgacaa tgacaatttg ccagataagc aacacctgcg cggcgttttg caggtgcgac
     4441 tcatccgggc gatgcaaata acgtaaaaat tcgtcaccgg ctgactttcc catttgatcg
     4501 accatcgact ccacgggtac gacatcgata ttcagccgat cgctgagacg gcgaatcgct
     4561 tttctggccc cggatgtcag cacccgaaaa ccaaccacaa acactaccgc cagcgtggcg
     4621 agcattatcc agatcatgag ttctcctgcg taatcagaac tcatgatatc tggaaaccct
     4681 cgccagttac taatccacca gaataatctt caatgcgaac agagctgcga ccaccaccac
     4741 acacaggttc agatcgcgcc agcgcccggt gcacactttc atgatgcagt acgacataaa
     4801 gccgagtgca atcccttcgg tgatcgagaa agtaaagggc atcatcaccg tggtaataaa
     4861 cgcaggcacc gattcggtaa aatcatccca gttaacgcgc gccaggctcg aagtcatcag
     4921 cacgccaaca aagattaacg ctccagcggt tgcgtaagga ggaaccatcg ccaccagcgg
     4981 tgagaagaac ataactaaca ggaacataac gccgaccacg acagccgtta gccccgtgcg
     5041 accaccgact gccacaccag aagtactttc aatataggca gtaacagacg aggtaccgat
     5101 aaatgcgcct gccaccgaac tgacgctatc aacatacagc gccttattca tattggggaa
     5161 tttaccgttg ctatctatta agcccgcttt atcagttaca ccaattaatg ttcctgatga
     5221 atcaaatagg ttgatcagca taaaggagaa aatgataccg gcgagttcaa gtgttaacgc
     5281 gccgctcaaa tctacttcac caatgacgcc gctaatatca ggcggaatgg aatagacgcc
     5341 gctaaaatga acatcaccga aaaataatcc acagcaagac gtcaccacaa tagaaaccag
     5401 caccgcggca tgaaaatgac gtgatgacaa cacggttata ataaaaaacc ctaaaatacc
     5461 taacaacacg ccgtgagaac ttaaatcgcc aatcatcacc agcgtgtctt tattggcgac
     5521 aataacacca gtgtttttta atcccattaa ggcaataaat aatccgattc cactggtgat
     5581 accaatacgt aaacttaatg gaatgttgga gatcatccag taccggatac gaaacagcgt
     5641 gagcaaaaat agcccaattg cgccccagaa tatcgcgccc atcccggtct gccaggagat
     5701 ccccatcgcc cccacgacca cgaacgcaaa gaaggcgttc agccccattg ccggagccag
     5761 caccacgggt aagttagcaa atatccccat cgcaatactg ccgataccgg caatcaaaca
     5821 ggtggtaaca aacaccactt tcgggtccat ttgtgccgcg ccgaggattt gcgggttcac
     5881 aaaaacaatg tacaccatgg ttaaaaaagt ggtcatcccc gcaatcaatt ctgtgcggac
     5941 ggtggtccca tgctgaggta gtttaaataa tcgcgaaagc gtccctgatt cattactcac
     6001 gtaatcggta ttgtcattat tcattttttt gtccattgga ggagatttaa tcccttgctt
     6061 ttgtaaaaca aatgacaaca acggttcagt gataattatc acatttcaat tgcacattaa
     6121 tagatattct ttaataatct cgcgacgttt ctttatgata aataataatc aaattgataa
     6181 aatcaaaatg agaaaaatat gaataattct attaaccata aatttcatca cattagccgg
     6241 gctgaatacc aggaattgtt agccgtttcc cgtggcgacg ctgttgccga ttatattatt
     6301 gataatgtct ctattctcga cctgatcaat gccggagaaa tttccggccc aattgtgatt
     6361 aaaggacgtt acattgccgg tgttggcgca gaatacgctg atactccggc tttgcagcgg
     6421 attgatgctc gcggcgcaac ggcggtgcca gggtttattg atgctcacct gcatattgaa
     6481 tccagcatga tgacgccggt cacttttgaa accgctaccc tgccgcgcgg cctgacgacc
     6541 gttatttgcg accctcatga aatcgtcaac gtgatgggag aagccggatt cgcctggttt
     6601 gcccgctgtg ccgaacaggc aaggcaaaac cagtacttac aggtcagctc ttgcgtgccc
     6661 gctctggaag gctgcgatgt taacggtgcc agttttaccc ttgaacagat gctcgcctgg
     6721 cgggaccatc cgcaggttac cggccttgca gaaatgatgg actaccctgc cgtaattaac
     6781 gggcagaatg cgctgctcga taaactcgat gcatttcgct acctgacgct ggacggtcac
     6841 tgtccgggtt tgggtggcaa agaacttaac gcctatattg ctgcgggtat tgaaaactgc
     6901 cacgaaagtt atcagctgga agaaggacgc cggaaattac aactcggcat gtcgttgatg
     6961 atccgcgaag ggtccgctgc ccgcaatctc aacgcactgg caccgttgat caacgaattt
     7021 aacagcccgc aatgcatgct ctgtaccgat gaccgtaacc cgtgggagat cgcccatgaa
     7081 gggcatatcg atgccttaat tcgccgcctg atcgaacaac acaatgtgcc gctgcatgtg
     7141 gcatatcgcg tcgccagctg gtcgacggcg cgccactttg gtctgaatca cctcggctta
     7201 ctggcacccg gcaagcaggc cgatatcgtc ctgttgagcg atgcgcgtaa ggtcacggtg
     7261 cagcaggtac tggtgaaagg cgaaccgatt gatgcgcaaa ccttacaggc ggaagagtcg
     7321 gcgagactgg cgctgtctgc cccgccatat ggcaatacca ttgcccgcca gccagtttcc
     7381 gccagcgact ttgccctgca attcacgccc ggaaaacgct atcgggtcat tgacgtcatc
     7441 cataacgaat tgattacgca ctcccgctcc agcgtctaca gcgaaaatgg ttttgatcgc
     7501 gatgatgtgt gctttattgc cgtacttgag cgttacgggc aacggctggc tccggcctgt
     7561 ggtttgctcg gcggctttgg cctgaatgaa ggcgcgctgg ctgcgacagt cagccatgac
     7621 agccataata ttgtggtgat cggtcgcagt gccgaagaga tggcgctggc ggtcaatcag
     7681 gtgattcagg atggcggcgg gctgtgcgtg gtacgtaacg gccaagtaca aagtcatctg
     7741 ccgttaccca ttgccgggct gatgagcacc gacacggcgc agtcgctggc ggaacaaatt
     7801 gacgccttga aagccgccgc ccgtgaatgc ggtccgttac ccgatgagcc gtttattcag
     7861 atggcgtttc tttctctgcc agtgatcccc gcgctaaaac taaccagtca ggggctattt
     7921 gatggcgaga agtttgcctt cactacgctg gaagtcacgg aataataaaa aaagcccggc
     7981 gtcatgccgg gcaaaagtca ccagttacgt ttatgccact gtcaactgct gaattttttt
     8041 ctcgcggcgg attttgcgtt cttccattac cgccactatc gccatcaggc agatacaacc
     8101 aatcgcggcg atatccagcg cggcgaaggt gcctgcccag ccggtaaggc cgaataccgg
     8161 cgtcccatcg gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa tcaggtaagc
     8221 aaaggtgcct ttaataccat cggcagcgcc aatcgctttt ttaggtacaa agccaacagc
     8281 agccacacca atcaacaatt gcgggccaaa gaccaggaaa cccaacgcaa agagagaagc
     8341 cagatagata tattcgttac tggcatgttg atacacaccg agcgtggcga taatcagcgc
     8401 cagcgcgatg caggccacca ggccacggcg accgttcgcc aggtcagaga gccagcccca
     8461 cagcagcgta ccgaccagcg caccagcttc aaacagcgta aagccctgaa tcgccaccgc
     8521 tttagagagt ttcagttcct ggaacgcgta tacggttgac cactggtcga taccaatacg
     8581 taccacatag aggaaaatgt tggcgaagca cagcagccag atcactttgt ttttcagcac
     8641 atactcaaca aagatctgcc acttggtcat atcggtagat tctgtctctt tgtcctcttc
     8701 gctgacctcc tcgccgaaca gttcttcagc tttgccgagg ccataagatt ccggggagtc
     8761 gctaccgtaa cgcaggccga taaaaccgac aatcagcgcg ataatcgacg ggaagataaa
     8821 catgccgatg acatggccat cgaacaggta atttgccccg aacagcgcca cacctgctgc
     8881 gcctgcaccg ccaaggttgt gagaaatatt ccagaaaccg aggaatgtcc cgcgtttacg
     8941 acgcggcgtc catttggtga tggtggagta actgcacgaa ccgccggtac tctggaaaaa
     9001 gccgcttaag gcgtagaagg caatcatcag gaacaggcta accgagccgc tgcccatact
     9061 ggcactgaag cccagcatac aaatggcaga gaggataagc atgaacggca ggaattgttt
     9121 ggtgtttttg ccgtcggcgt agtatgaaac caacgtttta cccacgccat aagtgatgga
     9181 gaaacccagg ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg aaatcatatc
     9241 gttctgcgcg atgttaaagt tcttgcgaat caggtacatc gtcaggtagc cgataaagac
     9301 caccaggtag gattgcatga acggtttgaa ccacattttg cgccgcactt cgagcggaag
     9361 gtccagggtc ggcttgcgaa cctggtttag gaaagccagc atgggttact cctgaaatga
     9421 atacctgcgt gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc
     9481 aagaaaaacc tggaaaattt cttagttttg ccggatatga agtgaaaagg tgagatgcat
     9541 cacgcttcac gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc ggaaatcccg
     9601 gcggcgatag cgataaccac aaaaaatccg ctccagtgcc aggtatcgag cactttcgcc
     9661 agcggccaac cagcaagcga cgcccccaga taagcaaaca agccgacaaa ccccgtcgcc
     9721 gcccctgccg cctctttgtg ggaacactct gccgccgcca taccaattaa catctgcggg
     9781 ccaaagacaa aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat
     9841 ggcatcagcc acagcgagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc
     9901 ggcccacggt tgccgttaaa caatttgtcc gagccccaac cggctaccag cgcaccgata
     9961 aatccgccca gttcaaacat cgtcactgcc gtattcgccg tgaccagatc gacgcccagc
    10021 gtctcggaca tatacaaatt gccccagtcg ttgatcgccg cccggaccac atagaccagc
    10081 acatagcaaa acgaaagcag ccagatatac ggattcagca acacatattt ggtgaggatc
    10141 tctttacgcg tcaaccctgc cccttcttgt tgttgagcaa tttccagcgc gtcgtgccgc
    10201 cattcaccga ccgccggtaa acctaacgcc tgcgggcgat cgcgtagccg ccagcagaga
    10261 aaaatcccca cgactatcgc catacaacca gcaatcatca tcccggcacg ccagccgtaa
    10321 tgcagcgcgc tcgctgccat cacaatggga atgagtgcgc cgccgacgtt atgcgccgtg
    10381 ttccataatg cccaccaacc gccgcgctcg gtacgtgaat accaggccgt taacagacgc
    10441 gcacacaccg gtgaacccca gccctggaaa aaggcgttca gcacccagag cacggcaaac
    10501 gcccatagcg acgtcgagaa gccaaacaga atgttcatga tgcccgtggc gataagccct
    10561 atccccataa aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac
    10621 acgccgtaag taatgtaaaa cagggtcgct aacaggccga tatcgctacg gctgagcacg
    10681 ccgttagcaa ggatttctgg taccgcggcg ttaaaacttt tccgcgtgaa gtaaaacagc
    10741 gcgtaaccca gccagatggt cagcagaata tgccgacgcc agtagcgata acgggcatca
    10801 atttcatgtt tatcagtcat taatggcgca tcggcaggcg ctttcagaaa cggcaacatc
    10861 atcgaacctt agacataacg ttgaggtaga gaaacgctga cacgcgtgcc gtgcagacag
    10921 gaaatgtgta atgtgccgcc cagcgccgtt acgcgctcgc gcattccggt gaggccaaaa
    10981 ccttgttgcc cggaacccgg cggcaaaccg ctgccatcgt cttcaatcac cagcatcaac
    11041 cgttcatcct gctgccagcc ttgcagcgta accgcgctgg catcggcatg tttcacaatg
    11101 ttgttcagcc cttcctggca gacacgaaac agcgtcacgc gctggttttc gcttaatgct
    11161 gattcatcga tacgccattc gagatggctg acaatgccgc gcccttccag ctccatttcc
    11221 cgcatcagtg agcggatggc ctgctccagg gtgagatcat ccagctggcg cggacgtaac
    11281 cgtcctaaca aacggcgcac cgcgtcgtaa acgcccagcg acagttgttc gatgagctgc
    11341 ccgctctgct tcacactggc gttatctgcc gccagccgct gaacaatgcc cgcctgagta
    11401 cgaatagcag tgatggtctg accgatatcg tcgtgcagct cacgcgccac gtcgcggcgc
    11461 acgctctctt cggtttctaa caaacgttca gccagatgct gattgcgcgc cagttccttt
    11521 tgtagcgact ggttaagttc acgcaaccgc tggatgcctg cgcccagtaa caaccctgtc
    11581 agactttgca ccagcaacga gagtaataaa tccaccagat gatcgcgcca ggtttgactg
    11641 gcgatcagcg cgatggcgtt catcaacgtc gcaatcagcg ctccttgcca gccatagtgc
    11701 caggccagcg cgataatcgg cagcgccaga cagaatggcg taaagcgcga cagttcgtcc
    11761 ggcaatccca actggagcca gagactgata acaaacagca gcaagtacca gaccagatgc
    11821 cgcccgcgcc agttgatggg ctgagaaacc agtgacgggc cgagcggcag ccaggtgtta
    11881 ttggcgagat agtgccagaa caccagacat atcggggcca gcgtcagacc gccagttaaa
    11941 gtcagcaaca gcgcattcca cgactctttg ccgtgccaaa gccagggcag cgactgcaac
    12001 aacgccgccg ccgttaacgc cgcaccctgt aacagcaagg tgcgccagtc acgctgatgg
    12061 cgatagcgcg agatcagcgc tacgggcagt aacgtcagta aactaccgat catcaataac
    12121 ggaaaatggg ttaaaccgac cgcctgcgtt agccagtaaa tcagcagcca ctccgcaccc
    12181 agcaatacgg gccagtatcc gcgcgggcat tgcagcatta gccccagacg cagaccaaac
    12241 ggaaataaca gcaccgccat atcagggcgc tcaaccagat gcaggctgat actccatagg
    12301 caaaaccatg cggcagagaa gataaaaaag caggcaataa cggtaattaa gcgggagaac
    12361 aacgtcttca tcaccagcca tcaaacatgc ggcgcgccag ctctacgtcg ttactgacgc
    12421 ccagtttttc catcagattg gcgcgatgga cgtgtaccgt tttcggtgac aagcccagtt
    12481 cggcggcaat ctctttcacc gccattcctt gcgccagttt ttccgccacc tggcgttcac
    12541 gtttggtcag cgggtcctga cgaccggatg ccagtttaat ggcaatatcc ggcgtcagat
    12601 aacagccgcc cgtggcaacc gtatgcaccg cagcaatgag ttcatccggg ctacagcgtt
    12661 tggaaagaaa gccgcgtgcc cccgcgttaa gcgcctgctc aaccagcgca ggactgtcgt
    12721 gaacggagag cataatcgtc gccatacctt tcggcagctg gcttagcagc tccagaccgg
    12781 agatatcggg catggagata tcgcaaatac acacctgcac accgcgcccc ggcagccccg
    12841 ccagcgcctc gcgccccgaa ccaaactcgg caactacctg caaatcaggt tccagcccca
    12901 gcagctgcgc aaagccggag cggacgatga ggtgatcgtc tataagggca acggtgatca
    12961 tggtcttgtc ctggcgggta aaaaaaaacg cgcttacctt aacgataagc gcgatgttgt
    13021 tcaagccttg agcggttact gaaaaaacac cgcgatcttg ttaaacatcg tcggatcgga
    13081 ctgattacgc tgcactttca cgacatcttc cagcttatcg atttggctta tcatctgctc
    13141 cagacgctgg tcgtcattga ccagtagcca gatatggctt ttgtcgctgt cctgaatcgg
    13201 cagacaaaga atgccttcaa cgttaaaagc gcggcgggca aaaaggccac aaacgtgggt
    13261 cattacgccc ggatggttgc gaacggtgag ctccagaatt acgttgttat gagttgtgtt
    13321 ttgcatggct tattccccca ccatttcagt attcgccgca cctggcggca ccatcgggta
    13381 aactttttct tcggcatcaa tgcgcacatg gatcagcgcc gggccagggc gattgattat
    13441 ttcctgcaat gcagcctgcg gatcggcttc gttattcaaa tcacaggttt cgaggccgaa
    13501 tccggcggca atctgcataa agttgatttt gcccggatag gtggcggcaa aaacgccttg
    13561 ctcgtagaac agactctgtt gctgatgcac cagccccagc gcttcgttgt tcatcagaat
    13621 gattttgaca tccagctgat tttcactggc ggtcgccatc tcctgaatat tcatcatcag
    13681 gctgccgtcg ccggagaaac acaacacttt gcgatccggg ttcgccagcg ccgcgccaat
    13741 cgccgcaggc aggccaaaac ccatcgtgcc cagcccaccg gaggtcagcc actggcgtgg
    13801 gcgattgagc ggataagctt gcgcggtcca catctgatgc tgacccacat cggtggtgat
    13861 aatcgcattg tcatcgacac aggcggcaac ggcgttgatc aggccgtaat ggcttaatgg
    13921 atcgcacgct ttcgggattg gacacgggaa ttcgcgctgc aaatccgcta ccaactggtg
    13981 ccactctgca cgcagttgcg cttccacctg cgggatcaat tgcgccagca cgtcatcaac
    14041 atccgcctga atcgccacat gcggctgctt gattttaccc agctctgcgc gatcgatatc
    14101 gacatgaata attttggcat tcggacagaa ctgctcggtt ttgccaatcg cccggtcatc
    14161 aaaacgcgca ccgagcacaa tcaacaaatc cgcctcttgc aaaatatagt tggtactgcg
    14221 cacgccgtgc atccccagca tacccagcga caacggatgc gcttttggca gcatgcccag
    14281 cgccattaaa gtcatggtgg taggcagttg cgctttctcc gccagttcac gcacccgcgc
    14341 aggcgcattg atcacaccac cacccagata aagcaccggg cgtttggcag cgttaatcat
    14401 tgtagctgcg tcacgaatgc tttcttcgct aaaggagggt gcagcggctt tttctgccac
    14461 cgcgggctga gcttcaatct caaaaaccgc cgtttgtaca tccttaggaa tgtctatcca
    14521 caccgggcta gggcggcctg attgcgcaat gcggaaggca tcgctcatga cctgtgggag
    14581 ctcttcgata tgtctgacca gatagttgtg tttggtgatg gggatagaga tgccgtaggt
    14641 gtccacttcc tggaaggcgt cggtgccgat catcgaggcg ggaacctgac cagtgatgca
    14701 aatcagcggg atggagtcca gccgcgcatc agcaatggcg gtcaccaggt tagtcgcacc
    14761 cggcccgcta caggccatac agaccgccgg tttaccgtcg gtgcgcgcca ttccctgagc
    14821 gataaacccc gcgccctgtt catggcgagc cagaatatgg cggatttgcg tgctttggct
    14881 taaggcgtcg taaacaggca ggatagaacc gcccggaatg cccgtcacaa tcttaatgcc
    14941 ctgctgttcc aggaaatgaa cgataaattc tgcgccggta aagcgcttac gcgtcgatgt
    15001 tgtgcccgaa cttgccatgc tccagtcctt ttcttctggg ccgactttcc gggaggtgct
    15061 taaacgaaaa accccgcccg gtttgcgccg gcggggtttt ggaatcgtgt gttgttccag
    15121 tccctacggc gcattgccga cgaccaccac cacacgcacg acgaccactg cggcggatgg
    15181 cgcagttggt agtagttttg cgttgaacat ggaagtagtc attggggacc ttgttggttt
    15241 tgtgtttaac aatatttata caagcacagc tttacagggg agacaatgga aaatttttca
    15301 gcaagggaaa attgaggggt tgatcacgtt ttgtactgaa ttgcagataa caaaaaaccc
    15361 cgccggagcg aggtttcgtc agccgcctgc ggctggtaac cgcaaagcac actgtattat
    15421 gtcaacactg aaagtatacg tgttccgcgc agaacgcgca atttcggcac gaattttgac
    15481 gtatttagtg catagttgag tatcgttcac agtttgcgtt ttgtccaaat attactgttt
    15541 atttatacag taaacttcta taatatcact gttcgcaatg tgttatgcgg gggccgcatc
    15601 gttacccggc gcactaagtc ctggctgaaa cgggtggtgc cgtcagcgcc ttaaccccgc
    15661 gtgagcacac tgtgttatgt caacaagcac aacgtttctc cttgagatac cgcgtgcaca
    15721 acagctggca acaggcagcg gaaaggtacg tcagctggca gtgctcctga accacaggag
    15781 acgcgtatga gcctggtgga tatcgccatt cttatcctca aactcattgt tgcagcactg
    15841 caactgcttg atgctgttct gaaatacctg aagtaattca gattcaagtc gcaccaaagg
    15901 ggagcgggta accgctcccc ttttatattt agcgtgcggg ttggagtcgg atgcgatgct
    15961 gacgcatctt atccgcccta ccatccctcc cggcaacatt tattgccgct tttgtttaca
    16021 tattctgccg ctaaacaatt ccccattcct ggcgtatatc tggctaacat tcatcaatgt
    16081 gatagattcc tctcccgcat ttatgggaat gcgtagtgac ttattctaat tatttttata
    16141 aaagcatccg tgataatgaa aaggcaaaga aacgtcaatt tgttattgat gttggtatta
    16201 ctcgtggccg tcggtcagat ggcgcaaacc atttatattc cagctattgc cgatatggcg
    16261 cgcgatctca acgtccgtga aggggcggtg cagagcgtaa tgggcgctta tctgctgact
    16321 tacggtgtct cacagctgtt ttatggcccg atatccgacc gtgtcggtcg ccgaccagtg
    16381 atcctcgtcg gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac gacctccagt
    16441 ttgacggtat tgattgccgc cagcgcgatg caggggatgg gcaccggcgt tggcggcgta
    16501 atggcgcgta ctttgccgcg tgatttatat aaacggacac agttgcgcca tgctaacagc
    16561 ctgttaaaca tggggattct cgtcagtccg ttgctcgcac cgctaatcgg cggtctgctg
    16621 gatacgatgt ggaactggcg cgcctgttat ctctttttgt tggttctttg tgctggtgtg
    16681 accttcagta tggcccgctg gatgccggaa acgcgtccgg tcgatgcacc gcgcacgcgc
    16741 ctgcttacca gttataaaac gcttttcggt aacagcggtt ttaactgtta tttgctgatg
    16801 ctgattggcg gtctggccgg gattgctgcc tttgaagcct gctccggcgt gctgatgggc
    16861 gcggtgttag ggctgagcag tatgacggtc agtattttgt ttattctgcc gattccggcg
    16921 gcattttttg gggcatggtt tgccgggcgt cctaataaac gcttctcaac gttgatgtgg
    16981 cagtcggtta tctgctgcct gctggctggc ttgctgatgt ggatccccga ctggtttggc
    17041 gtgatgaatg tctggacgct gctcgttccc gccgcgctgt tctttttcgg tgccgggatg
    17101 ctgtttccgc tggcgaccag cggcgcgatg gagccgttcc ccttcctggc gggcacggct
    17161 ggtgcgctgg tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc gctctctgcg
    17221 atgttgccgc aaaccggtca gggcagcctg gggttgttga tgaccttaat gggattgttg
    17281 atcgtgctgt gctggctgcc gctggcgacg cggatgtcgc atcaggggca gcccgtttaa
    17341 gcgcacgtca ccgcagcatc gtcatcagct ccatgggaga acgatgctgc tttatcagat
    17401 cacgcatcac ccgcatatgc ggtgcggagt aagaataaaa cgcctgatag cccgcacaaa
    17461 gcacgctgtc tggcgtctcc tgccgagcgt gcgggcacag gcgatgccag tcgcacgcct
    17521 ggcagtcacg gcgcggttgc gggttttcgc tgccacacca tttttgtaag gtggcgtcaa
    17581 aaagctcaat cgagatacga ctgatatctg cccgcaccca acactcaaac agggcaatta
    17641 aaaatttccc ccacagctct gctgtcaccg aagcggccgt cagatcgccc cggtcatcaa
    17701 attcaagatg gggaatgagc tgtacggagc ggatgccccg ctgtttgaaa ttgtgataaa
    17761 aatcggcagg ttgcagagca tccttgtact ctacaaccag cttatgcctg tcctcttcag
    17821 catcaatgac ctgacttcct gtcatgcgac tctctcaata aatacgataa gttgatgtat
    17881 atgcgttaca gcaaacagta ttgcgagaga taacaccgcg agggagatca aaaatttgtc
    17941 acggactacg tgaaattggg aaaaatcgct attcgtgaca tccattaaat tgcgattacg
    18001 ggtgtagtgc cagaggatca gcgccacgat ggcgaggatg ccaatcgaaa tccagaataa
    18061 catgcccgcc tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc cgtagcccag
    18121 catggtacga aaccacgcca gtgatgtgcg ctccggctgc agccctgggt cggcaatgcg
    18181 tctggcttta cggctatccg gcatacaaca ccagtcccat aacgatcacc gccacaacca
    18241 tcaatattaa gctgatgatt aataagctgt tggtgtaggg caggtcctct ttcagacgca
    18301 tcgctttttc attgcgtaac cagcgcaggt agccatacat tgctaacccg ccggaaaaca
    18361 gacacaacaa caacgccagc agttcacgaa tgacgggcgt ggcgaaatct ggcgcaagtt
    18421 gatcaagccc aacccccgcc gccagaaatc ctagtgcggt acggatccac gtcagaaaag
    18481 tacgctcatt agccagcgag aagcggtaat cgggcgcttc tccaaggcgg gaaattttca
    18541 ttgttgctcc tttgcaattt cactaacgct ctatctttag cggttttttt taagaatttc
    18601 gtccgcccgt ttcctgatat gcttttgcca tccgctacaa ggaggtcaga tgggtatcat
    18661 agcgcaaaat aaaatcagtt cattgggcat gctgttcggt gccatcgctt taatgatggg
    18721 gattatccac ttttcattcg gccctttttc cgcaccaccg ccaacgcttg aaagcatcgt
    18781 ggcggacaaa acggctgaaa ttaaaagagg tcttctcgcc ggaataaaag gcgaaaaaat
    18841 taccactgtg gagaaaaaag aggacatgga tatcgataaa atcctcgatc aaagcggtat
    18901 cgcactggcc attgctgcgc tactttgcgc atttattggc ggtatgcgta aggaaaatcg
    18961 ctggggcatt cgcggcgcgc tggtttttgg tatcggcatc gtgtgcagca ttttgctgat
    19021 attcctgatt ttttccttcc tgaccggcgg ttcgctggtt taagcagcct gaataatgcc
    19081 cgccgggtga tcatcagcgg gcattatcgt cagaatttct gttttttctc ctccaccttc
    19141 actccttgcg tcggtaggcc ggtgtcatag tcgcgtacca ctggcgaata gccatcttgt
    19201 gggcgtgggc gaaacgcccc catccagcgc ggtcgtgcat ctttacgcca cggacgcaga
    19261 ctccattggt aactgcggaa cggatcgcga attttgtcca tgtaatccaa taaggcgtca
    19321 tgcattttgc tgcgaacgtc tgcaaaacgg atatcatcga tcaggttatg catttcattt
    19381 gggtcattac ggcgatcgta aagttcatca ctggtgaaga ggttgagcac cagtttaaag
    19441 tcatccgtca cccagcaacg caccggaata aaaccgccaa agctgtcatg ctcaatctcg
    19501 tagcggttaa attccaccat cacgccgcgt ggctctttca cggcaaggat attttccccc
    19561 ggcagaatct ctggtttttc aatatctgcc agcgccatca ttgtcggcag taaatcaata
    19621 tgactgactg gcgtatcgac ctgtcgccgc tcctttactg atcagcttat gtgcgcccat
    19681 catttcaccg tgatcggagg tataaatgac ccacgtattt tcacgttgct ctggcgttaa
    19741 ggcattgatg acacgtccga tttggtcatc aacaaagtca ttgcaggcaa aatagagcgg
    19801 atggtgataa agcccgtcat caccgactgg cgatggcatc gcctgcgccc ataagcggtg
    19861 atgttccggt ttgttcgcca ggtcatcctg tgatttctcg ccaagttcgt agtaaaaatc
    19921 agcgtatttc tctaaatact ccaccggaca ggtgaacggg tgattcggct catcatacga
    19981 aaccaccatc aggaagggtt cctcggcgcg cacgggctgt tgcagaaaat ctaccgctcg
    20041 attgctgatg cggtgcgccc aggtgaaggt ttcgtcaata tggttcgcct gtaaatcttc
    20101 gacgctgttt aggccattgc gccacaggct gatctctttt tccgtcagtt cgctaagata
    20161 gttcgcccca tcaaaccagt aatcagcatc ccactccggc ggacactcgc cagtgccgaa
    20221 atagtcatga ccgtcgagat gccatttgcc gatgtaacag gtgtgatagc cggcatcctt
    20281 aaagtagcgc cccatagtgg agatgttttt gcctggcgcg acgttgttgg tccacgggcc
    20341 ggactggtta gcgtagatac cggtgaatag tccggcgcgt gcaggcgtac aaaccggtga
    20401 acaagtgtag gcggaattaa agcgaatacc ttccgccgcc agactatcaa tattttgcgt
    20461 attcagcggt ttaccgctat agcaaccgac catattggtg gcctgggtat cggtcaattg
    20521 gggcgtttca taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat
    20581 gccgccagct gtcaaaaatc aggtagacaa tcactacggc agcgatgaaa taactgatca
    20641 tcgctaacca gcgcgtaccg tagccgccga attcagccag cccgacatag acgccaatca
    20701 tcgcgaacag gataccaatt gacgcgatct tgacgttttt ccacggtttc atgtccaccg
    20761 caaacgcatc tttgaaggtg aacggcgtgg cgcgcggttt gataaaaccg atcaccagca
    20821 tcacgaccac gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact
    20881 tcaccagata gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg
    20941 ccagcgccgg gatgcgcggg aagaaaaagc ccataatgat gatggtaacc agcggcacgt
    21001 tgtaaatgcc gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc
    21061 acggcgctac caaaaccgaa acgatagcga taaagaaacc aaatttgcgc ccgacggtga
    21121 ccagctgctg cggctcggca ttctggttaa tgatgcgacg gtaaataccc atactgaata
    21181 atgtactggc gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg
    21241 cgccgaagaa acccaccagt ggcactggca gaacgttatt gaccagcgtc gggtaggcca
    21301 tgtcggcttt cggtagatcc tgatacagat gaaacgcaat caaccctggc agcaccagta
    21361 ccagcgggtc gagcattttc agcaccgccg ttaacagcgc ccccttttgc ccttccgcca
    21421 ggctttttga cgccagcgtg cgttgcacga tgccctgatt tgtacaccag taaaaggtgt
    21481 tcaccagaat taaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccaccga
    21541 ctgagtttaa tttctcggcg tgaacggtgg taatttgctc aatgccctgc ataaagctgc
    21601 ccttgcccat cgcgatcagg ccaaataccg gcaccatcaa cccgccgata accagcccaa
    21661 taccgttgat ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa
    21721 ttcccgccag accaagcaaa attaccagta gccagatagc cgcaccgtga gaaatctgta
    21781 gcgattcccc gacgtgaaac aggctgttga gcgccaacgc gccggagtag agaacaatcg
    21841 gcagaaagca gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt
    21901 tatcataacg ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg
    21961 gtaaaaagat tagcgcgagg aagatcagcg tcaccgctga agtcacttcc cagcccatca
    22021 ccgacatgcc gcttttgtag gcctgcccgg aaagaccaac cagttgttcc gtggaaaggt
    22081 tggttaacat taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat
    22141 agccttgttg cgatccggta tctgttttgc gaaccttcca ctaggtgatc accgccacca
    22201 gcagcgtaaa accgacaaaa ctcaagattt gtaacgaatt catctcatag cccttattta
    22261 tgtttatgtc ccggacgcat gaatataggg agaggtaaaa tgtgtcgggt caggcagtca
    22321 gattcttaaa atacaaacgt cgtatccctg aacggattgt tttctgttaa gttcaggttg
    22381 tgagcataat tcgccggagg gatattaaaa tgaatggaaa attgcaaagt tcagatgtaa
    22441 aaaacgaaac tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa
    22501 tttctctgat ctcgctctgg catacctacg ccgacgagca ttaccgggtg atctggccgc
    22561 gggacaagaa gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat
    22621 gtgggaaaat tcttttaaag aatggcgaac aaataactct gcatggcaac tgtattatat
    22681 ttttaaagcc aatggatatt cactcttatc actgtgaagg tttagtcttg gaacagtact
    22741 ggatggaatt tacccccacc agtatgatgg atattcccgt tggtcagcaa agcgttattt
    22801 ataatggcga aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag
    22861 aagcaataaa aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt
    22921 gtcttatgtc tgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg
    22981 ccactttaca tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc
    23041 cctgtagcga agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag
    23101 aatattacct cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact
    23161 cggttggcga agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct
    23221 ttaaacataa atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa
    23281 cggatgccag tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca
    23341 actcatccag aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg
    23401 cctgccacag gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg
    23461 ccacttgctg actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt
    23521 caggccgtag tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat
    23581 ccggctttca ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag
    23641 acgacgcgtg gcttctgcga caattgctgc cgggttggag tagttgagca tctaggcgtt
    23701 tggtgaatat ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc
    23761 gtacgctatt ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gccgtgggat
    23821 tttttcatcc agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca taacaaaatc
    23881 aacgtcgctg aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt
    23941 ttctttaagg atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc
    24001 ataaaatttc agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat
    24061 gcctggcgta aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact
    24121 gcctccgtaa tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt
    24181 tcacgcaatt gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag
    24241 acgccgtgcg ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc
    24301 agtgcaatac gtagacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc
    24361 agggcttgca ggataccggc agcctgatcc agctcttttt ttggctctgc agcggtggtt
    24421 tagcctcgcg aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt
    24481 cccggcgtgc acatgttgaa ctgcagaatc agggggcgga aaaccacgaa gtacagcagg
    24541 gtaaagcaca acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc
    24601 gggatccagt tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca
    24661 ccaaagagat acattacggt cgacattgag gccgccagca cggcgtgtac cgcaaacagc
    24721 aacggtgaaa tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg
    24781 gttaaggttg cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg
    24841 aagtacatcg ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca
    24901 aaaccgcctt ccgggaacaa cgatttcagc ggctcggcgc tcagactgaa ctcttgcaga
    24961 tgctgcgccc agtacatctg aatgccgcct tcaacagctg ccggaccaaa gataaacggt
    25021 ccgtagatga agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc
    25081 cacaccccaa gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccatttgt
    25141 acttttggcc agccgagcaa tgtcagccag gcacagggga tcatcaccag gaaggcgata
    25201 atcaccacat aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac
    25261 agacggttat gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta
    25321 tcgagggttt taatcccggc catcattgtc agaccgctac ctgccaccgc gtcctgagtg
    25381 aaatcgacgc cgaagtagct tccccaggtc attcccatcg cattgatgaa atagttccag
    25441 gtcaggaaac tcaccatcac cgccagacaa gcacgtccct gcgcttgctt agcaaggcca
    25501 atgggtaaac cgacagcaaa aatcagcggc atattacgga ataccgtcca accgccctct
    25561 tcaataatgt gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca
    25621 aacatcgggt tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc
    25681 agcactggcg tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc
    25741 tcttttctca attctgtaag taggaagcct gattggttat gcggcagacc tggagcgagg
    25801 ataggaagat gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc
    25861 ttttcgttgg gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg
    25921 tacacagcat cgaacacatc tttaaaaaaa agattttttt caatcgatta agcagaactt
    25981 gtgggcgcat tacccgtgct tgcaggcaaa aaagagagct agagatgatc tacaaaagca
    26041 ttgcggagcg gttaagaatt cgacttaatt ccgcggattt cacgctaaac ggccttcttc
    26101 ccggtgaaaa aaagctggcg gaagagtttg cggtatcgcg gatgactatc cgtaaagcca
    26161 ttgacctgct ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg
    26221 tgcgcaaaga tgtgctgcat caaaccgcca gcctgaccgg actggtggag gtgttaaaac
    26281 ggcagggaaa aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg
    26341 ccattgccag ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc
    26401 gttttgtgga agggaaaccg ctaatgctgg aagacagcta tatgccggta aaactgttcc
    26461 gtaacctttc actgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg
    26521 ggattttgat tggcggtaat tatgaaagcc tgatgccggt gctcgccgat agactgctgg
    26581 cgcggcaaat gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata
    26641 gcgagagcgg ggagtttttg aattattcag tgatgttcag aaatgccagc gaatactagg
    26701 tggagtacca tttacggcga ctccacccgg aaaagagtta accgatactc cagaagagca
    26761 ccgccagtaa ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt
    26821 aagagagcgc cgccgcacca ctggttggat gaagattatt agcaaacgcc agcgccggag
    26881 gatcggtcat ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca
    26941 tccgcgccag aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa
    27001 tccagcttag cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac
    27061 caacgaccga gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg
    27121 gcataaacca gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca
    27181 gcggaccgcc cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa
    27241 tagaacctaa caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt
    27301 gcagtttttg ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc
    27361 ccaccaggtt gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga
    27421 ccccggcacg gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc
    27481 gaatacgttt tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag
    27541 tgcctttcgt agacagcgag gtatcgacct cctgaccaat caccagttgc gcattatgta
    27601 aatccgctgg ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg
    27661 aaggtacttt tagggtttct tctcgtttca gacgcgagca gataattttg tcgccgttga
    27721 gaatcggtac atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga
    27781 tagtcttgat cagcgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct
    27841 cgacattgac gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc
    27901 cgaatggata cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac
    27961 ccaggtcgcg caagatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg
    28021 agaaaatccc cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa
    28081 ccagaccacc gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc
    28141 gcaatgaggc aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca
    28201 ggccaaattc ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa
    28261 caaaatggcc gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc
    28321 gaaatttgac gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg
    28381 ttaatgctat atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt
    28441 atggctgaat cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg
    28501 ccgaaatatc ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc
    28561 cccaccagaa tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc
    28621 tgatagcgat acgctgcgct gcgatgggtt ctggctcatt acgaattaaa tcaatatgca
    28681 gtaaaccgtt tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaaactca
    28741 ggctaaatgg ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct
    28801 ccggcgtgcc ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac
    28861 ggaaacctgc cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt
    28921 acggcgggaa gctctggctt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac
    28981 cgatccattg acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta
    29041 agaagcgagt aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg
    29101 gagatgtcag gccgcgccag gcggccttag ggaattagtt gatttcgata cggcgcggtt
    29161 ttttcgcttc cggaatcacg cgttcgagat cgatatacag caaaccattt accaggttag
    29221 ccccacgaac atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga
    29281 tgccctggta cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca
    29341 gcagattatc ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag
    29401 caatgcggta atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac
    29461 tctggctctg gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt
    29521 aaagcgggga taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta
    29581 agaccttcca caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcga
    29641 ctacgatgta aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg actttttttt
    29701 gcagtttatg gtctattgca tagactgagg gggcagcacg aaacgttaaa gtgcgatagc
    29761 cgccacagtg cgacgtaatg atgatgttat tttcagcaag ggatcgctat aactcatcag
    29821 gccaacaccc acgatcaaca gatgaattta ttatgataag aaatgtgttg ttagcgttca
    29881 tgatatgcag cggaatgaca ttactcggag gatgctccag cgtgatgtcc cataccgggg
    29941 ggaaagaagg aacgtatccc ggcacccgcg ccagcgccac catgattggc gacgatgaaa
    30001 ccaactgggg ctctaagtcg ctggcgattc tcgatatgcc gtttaccgct gtgatggaca
    30061 cgcttttact gccgtgggat gtgttccgca aagacagctc ggtgcgctcg cgcgttgaaa
    30121 aaagcgaagc caacgcgcaa gccaccaatg cggttattcc accggccaga atgcctgata
    30181 actgatttag cgatcggttt cggtgatcca cagctggcca ccttccatcc acgccagcca
    30241 ttgaccatcc ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt ggttttcggt
    30301 taaatactca acctcgccgc tttgcgcatg ggcacaaaca attcgattat ccagcacaaa
    30361 gcccaaccat tctcctgacg gatgccagtt aaatgcagac tgaatatccg ttttgttatg
    30421 ggttaacttg cgcggttcgc cgccctgtgg cgagataagc cacagttgca caatgccgtt
    30481 atcatcacgc attaaaaacg cgatttgcgt accctgcgga ttacagcgca cccagtggcg
    30541 cgggacgttg actaaccccg gataagcccg atgatgggta aaggttaaac gtcgctgcac
    30601 gacgccacgc ggtggcgcgg gtagggttgt ttccgttcca cttaacggcg catcacctgc
    30661 cgatttccag ctagcttcat cttgcggtaa ctcaacgata aatagctccg gcactttctc
    30721 gccctttggc gaaagtgtgt cgccaataaa cgccagcgcg tgatttccta cccatccttc
    30781 ttcataagca cgattgattt catcactgcc aggctgcggc gtgggcgtgg ttttactcac
    30841 cagcacacac cagtggctac cgctgtattc acgcggatgt tgcttttgta cgttgaccgg
    30901 gccaaacggt gcagcaaccc cgacgtttcg caaatccagc gccggatcga gttgatgcat
    30961 tacatggtcg ttataggtaa agctccccct ttcaccgttc gggctaaaga catgcacatg
    31021 gctgccaccg cgcagcgctc ctggcgtata cggcgcggta atatccattg catcgagatt
    31081 gctcaccttg ccgccttcag caatgactcc gcgtcgatga tggaagtcgt aatgccatgt
    31141 ttcatcagga ttttcagggc cgtgaataaa gacatatttc tctgacttcg gatgaacggt
    31201 caccacgccg acgtgtgcgc cctgtgacgc gcgatagata acctcgacct cgcctgtatg
    31261 gatattcaca cgctcaatgg tctcgccggt aaacgacgcc ccagaaggac gcacgtcaaa
    31321 taccagccac tggctgtcgg gcgtccaggt attggtattg gtgagcaggt gattacgggg
    31381 agcaaaggtt atctgtttca tggcgcggtc ctgatacaaa acggttcccg ccatcatact
    31441 tcacaaggac ttcactttca gacaatagtc ttttgccatc gtgatgaatg tgagtgaaaa
    31501 gatggaacat ttcgacgtgg cgattattgg cctcggcccg gcagggtcgg cgttggcacg
    31561 aaagttagcc ggcaaaatgc aggtgatcgc gctggataaa aagcaccagt gcggtactga
    31621 aggtttcagc aaaccttgca gattttcagc gtcaaaactg tcgacgtcgc cgcatcgctc
    31681 acgcgtaact accagcgaag ctatatcaat attaatcgcc atgctttcga cttgtggatg
    31741 aaatcgctga tccccgccag cgttgaggtt tatcacgata gcctgtgccg aaaaatctgg
    31801 cgtgaggatg ataaatggca tgtcattttt cgtgcagacg gctgggagca acatattacc
    31861 gcccgctatc tggtcggtgc cgatggcgca aactcgatgg tgcggcgaca tctctacccg
    31921 gatcatcaaa tccgtaaata tgtcgctatc cagcagtggt tcgcagagaa acatccggtg
    31981 ccgttctact cctgcatctt tgataatgcg ataactgact gttactcatg gagtatcagc
    32041 aaagatggtt attttatctt tggcggtgcc tatccaatga aagacggtca gacgcgtttc
    32101 acgacgctga aagagaaaat gagcgccttt cagttccagt ttggcaaagc ggtgaaaagt
    32161 gagaaatgca cggtgttgtt tccctcacgc tggcaggatt ttgtctgtgg taaggacaac
    32221 gcctttctga ttggtgaagc ggcgggattt atcagcgcca gctcgctgga ggggattagc
    32281 tatgcgctgg atagcgcaga gattctgcgt gcggtgttcc tgaaacagcc ggagaagagc
    32341 aacgccgctt actggcgcgc cacacgcaaa ctgcgtttaa aactcttcgg caagatagta
    32401 aaaagccgat gcctgaccgc accggcttta agaaagtgga ttatgcgcag tggtgtggcg
    32461 catattccac agttgaaaga ttatccaacg cgcttcacat cacccaccag caggatgtaa
    32521 gagagcgcgc caatcaacgc gacggcggag atataaacca gtgcaggcgc gaaaccgtaa
    32581 ccctgcgcca ggtaccccac caccagcgga acggtgatgc cgcccagacc accggcgaag
    32641 ttaaacacgc cgccggttaa accaatcagg cgcatcggtg ccagagaaga gaccagcgac
    32701 caggtaatcg aagcaaaacc gttgccgaag aatgccagcg ccatcaggca cataatcatc
    32761 atcggatcgt tggtgtagtt agcgcccata atgcaggtgg agatcagcaa gccgcagata
    32821 atcggcgttt tacgcgcaaa gcccagtgaa aagcccttac gtaccagcag atccgcgacc
    32881 cagccagaga gcaggacgcc gacaaacgcc gcgaggaatg gcaccgtggt cataaagcct
    32941 gctttcagcg ccgtgattcc tttttcctgg gttaaatagt tcgggaacca ggttaagaaa
    33001 aaccacagtg cagaagccac cgcaaattgc ccaagataaa cgccgatcag tttacgatgg
    33061 aacaccagtt tccagtcttt ggctgttaac ggctgacgcg cctctttctt caccggcgca
    33121 tcaccatcca ccagaccgcc gccatcacga atgtaatcca gttcagcttt gctgatacct
    33181 ttggtcaggc gcggcggctg ataaacctta aaccaaatca gcgaccagat aatgccgata
    33241 ccaccagtga caatgaacac ccagtgccag ctcaacatct cctgaatcca gatcagcagc
    33301 ggagtcaaaa acgccagacc gacaaactga ccagacgtat aaaaaccaac ggcggaagcg
    33361 cgttcatgtt ccggaaacca gctggtcacc atccggttat tggtcgggaa agcgggcgct
    33421 tcgaaaatac cggttatcgc acgcagacca attaatgaca ttaatcccgt ggcaaagccc
    33481 tggaataaag tcgccactga ccaaccaaat atcgcaataa aataagtcac gcgagaacct
    33541 acgcgatcta aaaaccaacc gccggggatc tggcatagcg tataaagcca ggcgaaggcc
    33601 gaaaatacat agcccatttc cgctttcgta atgccgaact cttcctgaat atgggcggaa
    33661 gccacggcca ggttagcgcg gtcaacataa caaatgacca ccgtaataaa gatcatcacc
    33721 agcgtcagat aacgccgacg ccccggcttt gctgcattaa cgggaatatc catagcgagc
    33781 tttctccaga ttttgggcat agcgaagccg ctcaccatgc cctgtaattt acagagggtt
    33841 atatttttgt attgctgttt tagtgcccga tgaggggctt acgtggcagg aattaccact
    33901 ctgctacgct gttatcttca tgacgccaga gcggattacg ccagtccggg gcatttttac
    33961 tgaactcaat aactttagct tcgtcgattt ccacacctaa gcccggtttc gttaacggtt
    34021 taaagaagcc gccaaccatg ctgaagtctt ctttgttttt cacaaagtcg agtaactccg
    34081 cgcctttgtt gtaatgaatg cccatacttt gttactgaag taccgcgtta taggaaacaa
    34141 agtcgatatg caggcaagcc gccagtgcaa tcggtccgaa cggacagtgc ggcgcaaggg
    34201 tcacatcata ggcttctgcc attccagcaa ttttgtagca ttcggtaata ccgcctgcat
    34261 gggagagatc cggttgcaga atcgaaatac cgcctgcctc cagcacgcgt ttaaaatcga
    34321 aacgtgagaa catgcgctca cccgccgcca gtggaatatg cgtttgtgcc gccaatttcg
    34381 ggtagtattc ggcttgttcc gccagcaccg gctcttcaat aaacagcggg cgatacggct
    34441 ccagttcttt aatcagcact ttcgccattg gcgcgctgac gcgaccgtgg aaatcaagac
    34501 caaactcaat ctgattgcca aaagcttcac gaatttgtgc cacggtgttg actgccgcat
    34561 ctaccgcgcg ggagttatca attagcccca gttcttcaca accgttcagt ttgaaggtat
    34621 cgaagccgat ttcgcgcagg gttttaatgc cgtcgataac atccgccgga cgatcgccgc
    34681 cgacccaact gtaggcttta attttgtcgc gaaccaggcc gcccatcagt tgccagactg
    34741 gcgcattcag cactttgcct ttgatatccc ataacgcctg gtcgatcccg gcgatggcgc
    34801 tcatcaggat tggaccgcca cgataaaatc cggcgcgata catcacttgc cataagtcgt
    34861 tgatgcgcga aggatcctga ccaatcaaat agtcacccag ctcgtgaact gccgcttcca
    34921 ccgtacgggc gcggccttca atcacgggct cgccccaacc gaccacgcct tcatcggttt
    34981 caattttcag gaacatccag cggggaggta aacgatacgt ggtaattttg gtgattttca
    35041 ttgcactgcc tctcgatacg ccttaacaaa tgctgctgcc tgctgcgcgg tgcgttctac
    35101 ggactgcccg gcgcgataaa gatcgctgcc taagcccgcc ccaacacaac ctgcgtctat
    35161 ccactgcgcc aggttttctg gcgtcacgcc gccaacggca aagactgcga tgtccggtgg
    35221 taataccact tttaacgctt tgatgtattg cggaccaaaa gccgatgacg gaaatatttt
    35281 cagcgcctgc gcgcccgctt cgagcgcggt aaaggcttcg gtcgccgtcg cgcagccggg
    35341 gcaaacggtc atgccgtagc ccaccgcgcg gcggatcact tcactatgga tattgggcgt
    35401 aacgatgagc tgacagccca tcctggcgag cgcatcgacc tgttcaggtt tcagtaccgt
    35461 acctgcgcca atcaacgcct tgtcgccata cgcatcaacg atggcgggaa tgctctgctc
    35521 ccattgtggg gaattcagcg ggatttcaac cgcgtcgaac ccggcgtcaa tcaccgcgcc
    35581 aacatgcgcc agcgcctcgt cgggcgtaat gccgcgcaaa atggcgatca gcgggagttt
    35641 agtttgccac tgcatgagcg atgctcctta taccagcctg aaatgccgtg tcgcccgcca
    35701 ccgccgccac gtcgcaaccc atcgcctgaa aggcttgctg gtagcgcgcg gtcagcgatg
    35761 ttccggcgac aagggtgatg gcgtgttgat gggtcacata gtcgcgcata ctggcaacct
    35821 ctgcgccaat caacaaacca gagagaaatt cgctgacctg ttcgcggaga agtgttccca
    35881 gcacatgcga ggcgcgaact tcaaaaagct gcggcaatat ggcgggcgca ttaaggccgc
    35941 gctcaaggcc agccgcgaag gcatcggcag agttttcctg cggcggcaaa cctgcgccaa
    36001 tcaatgagtg atttaacagt aaatgatgta attcaccggt catcacggtg cgaaaatcgt
    36061 tgatttgctg gctatcggcc tgcacccatt tgcaatgcgt accgggcata acataaagag
    36121 aggaaggagc cagagctcgc gcgccgatca attgtgtttc ttcgccgcgc atcacattgt
    36181 ggttatcgtc atgagagacg cataatccgg gaataatcca gatattgtcg ccaacagacg
    36241 ttaattgttc gccaatagac gaaaaacggg caggaacaga taaatacgga gcaactttcc
    36301 agccgacatt gctgccgacc attcctgcca tcaccactgg cgtattttct tcacgccagt
    36361 cggtcgtgac ttctgctaac accgcagccg gagattttcc gttcaggcgc gtgacgcctg
    36421 cttctgattg cctgctctcc aggcagtggt cgccctgata aagccaggcg cgcagattgg
    36481 tcgatcccca gtcaattgcg atgtagcgag ctgtcatgtg atttccttta accttcgtgt
    36541 cgagctggca atcatggtaa gcgccgcctg ctctgccgca tcgccgtcct gatgccgtat
    36601 cgcatcgaac agcgccttat gttcctggag cgtttgcggc atgttggcct catcgcccat
    36661 ccaggttcgt tcaaaaaccg cccgctgcag cgaactgatc gcaatgctaa gttgctgtaa
    36721 caccgggtta tgcaccgact gcagcaccgc ctcgtggtag cgaatatccg cttcgttaaa
    36781 cgcttcgcgg tcctgattgt tggcaatcat ctcgttcagc gccgattcaa tctgcgccag
    36841 atcgctggag gtcgcgcgct ctgctgccca acgggcaatc gccggttcca ccagatttcg
    36901 cacttcgctc atggcactga taagccgtgg gtcgtagtcg ttttccagca cccattgcag
    36961 tacgtcagtg tcgaggtaat tccactggtt acgcggtgcc acaaacgccc cgcgataacg
    37021 tttcatttca atcagccgct tcgccatcag cgaacggaac acctcacgga tgatgttgcg
    37081 cgaggttgca aactcttcac agagctccgc ctcagccgga agtggcgagc caggtacgta
    37141 tttgccgtga acgatctgtt tacccagcgt aatgacaatg cgatcggttt tattgagagt
    37201 catggagagt ccttgtgctc tgtatgttct tctctacttt accccgatcg atgcataacg
    37261 cggcaacttt gtagtaccag cgtgatgacg ttcgcgtttg ccgtgcgtgt aatgtagtac
    37321 aaacttatat tgttgtgcta caatttagat caaaaaaaga acaatgtata aaaaaatgac
    37381 atgcgtcggg cagaaatctg aaaagggata tcaggcgcta aacaggaggg aaagaagagt
    37441 atgctttcaa cggcttagct actcgtttaa aagattaatc atgaagttga attttaaggg
    37501 attttttaag gctgccggtt tattcccact ggcgctgatg ctttcaggct gtatctcgta
    37561 tgctctggtt tcccataccg caaagggtag ttcaggaaag tatcaatcgc agtcagacac
    37621 catcactggg ctatcgcagg caaaagatag taatggaaca aaaggctatg tttttgtagg
    37681 ggaatcgctg gattacctta tcactgatgg tgccgatgac atcgttaaga tgctcaatga
    37741 tccagcactt aaccggcaca atattcaggt tgccgatgac gcaagatttg ttttaaatgc
    37801 ggggaaaaag aaatttaccg gcacaatatc gctttactac tactggaata acgaagaaga
    37861 aaaggcactg gcaacgcatt atggttttgc ctgtggtgtt caacactgta ccaggtcact
    37921 ggaaaaccta aaaggcacaa tccatgagaa aaataaaaac atggattact caaaggtgat
    37981 ggcgttctac catccattta aagtgcgatt ttatgaatac tattcaccca gaggcattcc
    38041 ggatggtgtt tccgcagcat tactgccagt gactgttacg ctggacatca ttactgcacc
    38101 gctgcaattt ctggttgtat atgcagtaaa ccaataatca gtaagcgggc aaacgcgttt
    38161 atgctgtttg cccgcccaca gattaattca gcacatactt ctcaatagca aacgccacgc
    38221 catcttcaag gttggatttt gtgacaaagt tcgccatttc tttcaccgaa ggaatagcgt
    38281 tatccatcgc cacaccgacg cctgcatatt caatcattgc gatgtcgttt tcctgatcgc
    38341 caatcgccat gatttcttcc ggtttaatac ctaacacgtc ggccagtgat ttcacacccg
    38401 tacctttgtt aacgcgttta tcgaggattt cgaggaagta cggcgcactt ttcagcacgg
    38461 tatatttctc tttcacttcc tgcggaatac gcgcgatagc ctggtcgagg atggcgggtt
    38521 catcaatcat catcactttc aggaactggg tattggggtc cattttctcc gcttcgcaga
    38581 acaccagcgg aattgtggca acgaaggatt catgcaccgt gtagtagctg atatcgcggt
    38641 tagcggtgta cagcgtggtg cggtccaggg cgtggaaatg agaaccgact tcgcgagaga
    38701 gtttttccag gaaacgatag tcgtcatagc tgagagcggt ttgcgccacg gtgctaccat
    38761 cagcggcctt ctgtaccagc gcgccgttat aagtaatgca gtagtcgccc ggctgttcca
    38821 tatgcagctc tttcaggtag ttgtgcacac ctgcatacgg acgacccgtc gttagcacga
    38881 cattcacgcc acgggcgcga gctgcggcaa tcgcattttt aacggcgggt gaaatggtgt
    38941 gatcgggcag cagaagggtg ccatccatat cgatagcaat gagtttgata gccatgagtt
    39001 cccaaggtag attagttcct gacccatgct aacgcgattc cgctcaaaaa tcagtacaac
    39061 acccgaggga aaaggggatg caacgcgggt tgcatgctcc ccatttgctt agcggaagag
    39121 tttccctttc agcagttcca tgcctgcgga aagcagatcg ttattggctt gtgctgacac
    39181 ttcaccttgc ggtgagagcg catcaataat cttcggcaat tgctctgcca gtaaactgga
    39241 agccgtactg gtatccacgc caagtttttg cccgagatcg gacaccgcat ttgtgccgag
    39301 cgccgattcc agttgctcgc cgctaactga ttgatttctc tgttgattac tcaaccaagt
    39361 tgagagaatg gcccctaagc cgccactttg cagtttttcc agcagcacct gaatgccacc
    39421 ctgctcctca acccaactta aaatcgcctg atatttcccc gcatcgcctt tcagaaaggc
    39481 accgacaact tcatcaaaaa gccccatgat aatcacctgt aaagcgttac gtgttgaccc
    39541 aaaaagtata gatctgcggg atgataattg cggattgcag aaataaaaag ggcggagatg
    39601 atctccgccc ttttcttata gcttcttgcc ggatgcggcg tgaacgcctt atccggccta
    39661 caaaatcatg caaaatcaat atattgcagg agtctcgtag gactaataag cgtagcgcat
    39721 caggcacgct cgcatggtta acgccattaa atatcaatat tcgccgcttt cagggcgttc
    39781 tcttcaataa acgcacgacg cggttctacg gcgtcgccca tcagcgtggt gaacaactgg
    39841 tcagcggcaa tcgcatcctt aacggtaacg cgcagcatac gacggctttc cgggtccatg
    39901 gtggtttccc acagctgttc cgggttcatc tcgcccagac ctttataacg ctggatggag
    39961 aggccacgac gggactcttt caccagccag tccagcgcct gctcgaagct ggctaccggc
    40021 tgacgacgct cgccacgttc gataaacgca tcttcttcca gcaagccacg cagtttctca
    40081 cccagcgtgc agatacgacg atattcgcca ccggtgataa actcgtgatc cagcggatag
    40141 tcagtatcca caccgtgggt acgcacgcga acaatcggct cgaacaggtt ttgttcggca
    40201 ttggtgtgga catcaaactt ccactggctg ccgtgctgtt ctttgtcgtt cagttcgctg
    40261 accagcgcgt tcacccagcg ggtaacggtc tgctcatcag aaaggtcagc ttccgtcaac
    40321 gtcggctgat agataagctc tttcagcatt gctttcggat aacgacgctc catgcggttg
    40381 atcattttct gcgtcgcgtt gtactcagat accagtttct ctaacgcttc gccagccaat
    40441 gccggtgcac tggcgttggt gtgcagcgtt gcgccgtcca gcgcgataga gatctggtac
    40501 tgatccatcg cttcgtcgtc tttaatgtac tgttcctgct tgcctttctt cactttgtac
    40561 agcggcggct gagcgatgta gacgtgaccg cgttcaacga tttccggcat ctgacgatag
    40621 aagaaggtca acagcagcgt acgaatgtgc gagccgtcga cgtccgcatc ggtcatgatg
    40681 atgatgctgt gataacgcag tttgtccggg ttgtactcgt cacgaccgat accacagcca
    40741 agcgcggtga taagcgtcgc cacttcctga gaagagagca tcttatcgaa gcgcgctttc
    40801 tcgacgttaa ggattttacc cttcagcggc agaatcgcct ggttcttgcg gttacgcccc
    40861 tgcttcgcag agccgcccgc ggagtcccct tccaccaggt acagttcgga aagcgccgga
    40921 tcgcgttcct ggcagtctgc cagtttgccc ggcaggccag ctaagtcgag cgcaccttta
    40981 cggcgggtca tttcacgcgc gcgacgcgcc gcttcacggg cacgggcagc atcgataatt
    41041 ttgccaacca cgattttcgc atcagttggg ttttccagca ggtattccgc cagcagttcg
    41101 ttcatctgct gttcaaccgc cgatttcacc tcagaagaaa ccagtttgtc tttggtctgg
    41161 gaggagaatt tcgggtccgg cacttttacg gaaacgaccg caatcaggcc ttcacgcgca
    41221 tcgtcaccgg tggcgctgac tttggctttt ttgctgtagc cttctttgtc catgtaggca
    41281 ttcagggtac gggtcatcgc cgcacggaag cctgccaggt gagtaccgcc gtcacgctgc
    41341 ggaatgttgt tggtaaagca gtagatgttt tcctggaagc catcgttcca ctgcagcgcc
    41401 acttcaacgc caataccgtc tttttcggtg gagaagtaga agatatttgg gtggatcggc
    41461 gttttgttct tgttcagata ttcaacgaac gctttgatgc cgccttcata gtggaagtgg
    41521 tcttctttgc cgtcacgctt gtcgcgcaga cgaatggaaa cgccggagtt gaggaacgac
    41581 aactcacgca gacgtttcgc cagaatttca tattcgaact cggtcacatt ggtgaaggtt
    41641 tcgaggctgg gccagaaacg caccatggtg ccggtttttt cagtctcgcc ggtaaccgcc
    41701 agcggagcct gcggtacacc gtgttcgtag atctgacggt gaattttacc ctcgcgctga
    41761 ataaccagct ccagtttttg cgacagggcg tttactaccg aaacaccaac gccgtgcaga
    41821 ccgccggaca ctttatagga gttatcgtca aatttaccgc ctgcgtgcag aacggtcatg
    41881 atcacttccg ccgccgatac gccctcttcc gggtgaatac cggtcggaat gccgcgcccg
    41941 tcatcctgta cagagacaga gttatcggcg tgaatggtga cgataatttc tttacagtga
    42001 cccgcgagcg cttcgtcgat agcgttatct accacctcga ataccatgtg gtgcagaccg
    42061 gtgccgtcat ccgtgtcgcc gatatacata cccgggcgct tacgcaccgc atccagccct
    42121 ttcaggactt tgatactgga ggagtcataa gaattcgaca tcaacgtttc tcgctcattt
    42181 atacttgggt taatccgtta ttttaccctt ttccacggta aacatcttcg aattttcgtc
    42241 cgacatgtct ataacgtgtt cagcactgat cgcgctgaca aagacctgtg attgcgtcgc
    42301 ttttaagcgg ctggcaagca acccgcgacg ctcatcatca agctcagagg caaaatcatc
    42361 tatcaggtag agacaccgcc gcccgctttc acgagtgagg aactctcctt gcgccagacg
    42421 taaggcgcac atcaacagct taagctgccc acgcgataag gtatcttcca ccggcgcacc
    42481 gtcggcgcga atgcgtaagt ccgctttatg cgggccatgc gcggtgtagg ttagctggcg
    42541 atcgcgttca aaattacgtt ccagcacctc agcgtactct gtctctttct cccagccgcg
    42601 ctggaaagag aaagtcagag aaaactcagg gagaaattgc ttacaggtat catccatatc
    42661 ggccgcgata ccggcgctat actccgcgcg ccaggtgctg atttgctccg ccagcgggat
    42721 cagttcttta tcccacgggc gtagctgttc gtaacgagtc acctggcgca gcgccgcatt
    42781 gcgctgcttg agcaatcgct tgagattgct ccaggcggtg aaaaatctag gttcgttgtg
    42841 aaagcatccc cagtcgagga atgctcttct gtatttgggg ccgccgttga gtaaagtaaa
    42901 cccttctggc gttatcaact gcatcggcat caggtgcgcc agttccgcga ctttatgccc
    42961 gtccgtaccg tcaatacgga ctttgctgtc gccctgtttg tctttggtta agccaatcgc
    43021 cgtctcgcgc tcttcgccct gtaatcgccc gtggagaaca aatgcctcct gctcatggcg
    43081 aatgacgcga ccaatctgca aactgcgaaa cgcccgacca tggccgagcg tatagatggc
    43141 ttccagcacg ctggttttgc cactgccgtt ggcacctacc agaaagttaa agccgggaga
    43201 taaagcgaga tccgcggttt caatattgcg gaaatcgcgg atcaacaagc gggtgaggga
    43261 cattacagtc tcattggcat gacaacatag gcagccgact gtgatgcggc atcttcaatc
    43321 tgcacgctgg aaaccgaatc ggtcagcatc atgcggacgt tttcgcattt cagcgcgttc
    43381 agaacatcca gcacatagct gacgttgaag ccgatttcca tctccgcacc gctataggta
    43441 acgtcgagga tctcttccgc ttcttcctgt tccgggttgt tggcagtaat tttcagctgg
    43501 ttttcgctga catacaggcg cacaccgcgg aatttctcgt tagagagaat cgccgcgcga
    43561 gcaaacgcct gcttgagcag atcgcagcct gcttccagat gtttgtccgg gttcttcggc
    43621 agaacgcgac ggtaatccgg gaagcgacca tccaccagtt tcgaggtgaa aataaagtcg
    43681 ccaacgtggg cacgaatgtt attgctgcca atctgcacac gcagcagatt gtcgccgccg
    43741 tcgagcatac gcatcagttc aatcacgcct ttacgcggta cgatcaccga atggcttggc
    43801 aaagattgac caattggcat tgaacagacc gccagacggt ggccgtcggt tgccacggtg
    43861 cgcagttctt caccttcggt ttcaaacagc ataccgttta agtaatagcg aacatcctga
    43921 tgcgccatcg aaaactgggt tgcttcaatc agacgcttca tcgtcgcctg cggcagggta
    43981 aattcgactt cgctctgcca gtcatcgagg ttcgggaaat ccgccgctgg cagggtagac
    44041 agcgaaaaac ggctacgccc ggagcgcacc agcatccgtt caccttccag ctgcacggca
    44101 atttccgcgc cttcaggcag accacggcag atatcaaaga atttgcgcgc cggaacggtc
    44161 gtcgctcctg gctcgtgtgg ctgaaccagc gcaacgcgtg ccaccatttc catctcgaga
    44221 tcagtaccgg tcagcgacaa cgtaccgtca gcaacctgta acagcagatt accgagaatc
    44281 ggtagcgtag gacgaccacc taacggaccg ctcacctgtt gtagcggttt taataaatgc
    44341 tcacgttcta cggtaaattt cataggttta cgatgacaat gttctgatta aatttgaaaa
    44401 atcttctttg atatcgtggc tctcttcacg caactgctcg atcttacggc aggcatgaag
    44461 caccgtcgtg tggtcacgcc caccaaacgc atcgccaatc tctggcagac tgtggttagt
    44521 cagctctttc gctagcgcca tcgccatctg gcgcggacgc gccaccgagc gggatcgacg
    44581 cttggaaagg agatccgcga ctttgatctt gtagtactcc gccaccgtct tctgaatatt
    44641 gtcgatggtg accagttttt cctgcaatgc cagcaagtcg cgcagcgcct cacgcacgaa
    44701 gtcgatggtg atcgcccgtc cggtaaagtt ggcgttagca ataacgcggt tcagcgcccc
    44761 ttccagctca cgtacgttag atcgtagacg cttggcgata aagaacgcca cttcacccgg
    44821 caaacgaatg tcattttcgt cggccttttt catcaggatc gccacacggg tttccagctc
    44881 tggcggttcg atcgccacag tcagtcccca accgaagcgg gatttcaaac gatcctcaac
    44941 gccgttgatc tctttcggat agcgatccga ggtgagaatg atctgttgat taccttccag
    45001 cagggcgttg aaggtgtgga aaaactcttc ctgagatcgt tctttattag caaaaaactg
    45061 aatatcgtcg atcagcagtg catctacgga acggtagtag cgtttaaact cttcgatcgc
    45121 gttgttttgc agggctttaa ccatgtcctg aacaaagcgc tcggagtgca tataaaccac
    45181 tttggcattc ggcttgcgcg ccataatgcc gttacccacc gcatgcagca ggtgagtttt
    45241 acccagaccc gtgccgccat aaaggaacaa cgggttatag gcaccgccag ggttatccgc
    45301 cacctggcga gccgccgcgc gcgccagttg gttagattta ccttcaacga agttatcaaa
    45361 cgtgtgtttg acgtttacgt tagaacgata ggtcggttct gccggggccg ggacgttatc
    45421 ccaacctgag cgcgtagaag gcgcagcacg ttgcggctgc gtttgcgcca cctgtgcagg
    45481 ggccgcgacg ttgctcgtca ctgccgcttg tggcgtttgc gtcaccggtt tggtgccgac
    45541 ttcaaaacgc agctgtgggg catccgctcc gcagaaactg gttagcagtc cattgatatt
    45601 attaaggtac ttgtcccgta cccaatcgag gacaaaacgg tttggcgcgt acagggacag
    45661 cgtgttatcg ctcagttccg cctgcaatgg gcgtatccac atactgaatt ctgtggctgg
    45721 taactcatcc tgcaatcggg caagacactg ctgccaaagc gaaagtgaca cggcggactc
    45781 cactcgaaca aaagtcgata atgactaagg ctgaaacatt catgattgtt gacgtacgtc
    45841 gaaaagaccc tgcttgaggg tgacgcacga accgctgtct gcggttatat gcccgatcaa
    45901 gatcctgcaa aacgatcggg accgcggatc atagcctaaa ctgcgcaaga gatcttctgt
    45961 ttctcacaga ttttccctat ttatccacag gactttccag aactcgctaa gtgtaaacga
    46021 tcctgccgcg aggcgggcac gatttacgcc gcatattgga aaatttaatg accatagaca
    46081 aaaattggct taatcgatct aataaagatc caggacgatc cttgcgcttt acccatcagc
    46141 ccgtataatc ctccacccgg cgcgccatgc tggtttccac tggtgtgagg ccgtacattt
    46201 tccctgcgaa aaggtgcgga aaagcgcggt aaataaggaa agagaattga ctccggtgtg
    46261 tacaattatt acaatccggc ctctttaatc acccatggct tcggtgtcca tcgtttcatt
    46321 tttcggcgga tatccaataa agccattgaa tttattcaag tttaggtaga aatcgccatg
    46381 aaacgcactt ttcaaccgtc tgtactgaag cgcaaccgtt ctcacggctt ccgtgctcgt
    46441 atggctacta aaaatggtcg tcaggttctg gcacgtcgtc gtgctaaagg ccgcgctcgt
    46501 ctgaccgttt ctaagtaata aagctaaccc ctgagtggtt aagctcgcat ttcccaggga
    46561 gttacgcttg ttaactccca gtcaattcac attcgtcttc cagcagccac aacgggctgg
    46621 cacgccgcaa attaccattc tcggccgcct gaattcgctg gggcatcccc gtatcggtct
    46681 tacagtcgcc aagaaaaacg ttcgacgcgc ccatgaacgc aatcggatta aacgtctgac
    46741 gcgtgaaagc ttccgtctgc gccaacatga actcccggct atggatttcg tggtggtggc
    46801 gaaaaaaggg gttgccgacc tcgataaccg tgctctctcg gaagcgttgg aaaaattatg
    46861 gcgccgccac tgtctcctgg ctcgcgggtc ctgatagccc tcattcgggt ctatcaacgc
    46921 ctgattagtc cgctactcgg gccgcattgt cgtttcactc caacctgttc aagctacgga
    46981 attgaggcat tgcgcaggtt tggagtgata aaaggcagtt ggttgacggt gaaacgcgta
    47041 ttaaaatgcc accctttaca ccctggtggt gacgatcccg tcccgcccgg accatttgat
    47101 accagagaac actaacgatg gattcgcaac gcaatctttt agtcatcgct ttgctgttcg
    47161 tgtctttcat gatctggcaa gcctgggagc aggataaaaa cccgcaacct caggcccaac
    47221 agaccacgca gacaacgacc accgcagcgg gtagcgccgc cgaccagggc gtaccggcca
    47281 gtggccaggg gaaactgatc tcggttaaga ccgacgtgct tgatctgacc atcaacaccc
    47341 gtggtggtga tgttgagcaa gctctgctgc ctgcttaccc gaaagagctg aactctaccc
    47401 agccgttcca gctgttggaa acttcaccgc agtttattta tcaggcacag agcggtctga
    47461 ccggtcgtga tggcccggat aacccggcta acggcccgcg tccgctgtat aacgttgaaa
    47521 aagacgctta tgtgctggct gaaggtcaaa acgaactgca ggtgccgatg acgtataccg
    47581 acgcggcagg caacacgttt accaaaacgt ttgtcctgaa acgtggtgat tacgctgtca
    47641 acgtcaacta caacgtgcag aacgctggcg agaaaccgct ggaaatctcc tcgtttggtc
    47701 agttgaagca atccatcact ctgccaccgc atctcgatac cggaagcagc aacttcgcac
    47761 tgcacacctt ccgtggcgcg gcgtactcca cgcctgacga gaagtatgag aaatacaagt
    47821 tcgataccat tgccgataac gaaaacctga acatctcttc gaaaggtggt tgggtggcga
    47881 tgctgcaaca gtatttcgcg acggcgtgga tcccgcataa cgacggtacc aacaacttct
    47941 ataccgctaa tctgggtaac ggcatcgtcg ctatcggcta taaatctcag ccggtactgg
    48001 ttcagcctgg tcagactggc gcgatgaaca gcaccctgtg ggttggcccg gaaatccagg
    48061 acaaaatggc agctgttgct ccgcacctgg atctgaccgt tgattacggt tggttgtggt
    48121 tcatctctca gccgctgttc aaactgctga aatggatcca tagctttgtg ggtaactggg
    48181 gcttctccat tatcatcatc acctttatcg ttcgtggcat catgtacccg ctgaccaaag
    48241 cgcagtacac ctccatggcg aagatgcgta tgctgcagcc gaagattcag gcaatgcgtg
    48301 agcgtctggg cgatgacaaa cagcgtatca gtcaggaaat gatggcgctg tacaaagctg
    48361 agaaggttaa cccgctgggc ggctgcttcc cgctgctgat ccagatgcca atcttcctgg
    48421 cgttgtacta catgctgatg ggttccgttg aactgcgtca ggcaccgttt gcactgtgga
    48481 tccacgacct gtcggcacag gacccgtact acatcctgcc gatcctgatg ggcgtaacga
    48541 tgttcttcat tcagaagatg tcgccgacca ctgtgaccga cccgatgcag cagaagatca
    48601 tgacctttat gccggtcatc ttcaccgtgt tcttcctgtg gttcccgtca ggtctggtac
    48661 tgtactatat cgtcagcaac ctggtaacca ttattcagca gcagctgatt taccgtggtc
    48721 tggaaaaacg tggcctgcat agccgcgaga agaaaaaatc ctgattcggt gagttttcgc
    48781 taaaataagg gcggtcagtt gaccgccttt tttcttttcg tagggcggat aagcaccgcg
    48841 catccgccac acaaagcaac aggaacatca tgagcgataa tgacactatc gtagcccagg
    48901 ccacacctcc gggacgtggc ggcgttggca tcctgcgcat ctccggcttc aaagcccgtg
    48961 aagttgccga aaccgtgctg ggtaaactgc ctaagccgcg ctacgccgat tatcttccgt
    49021 ttaaagacgc cgacggcagc gtgctcgatc aggggattgc gctatggttc cctggcccga
    49081 actcgttcac cggcgaagat gtgctggaac tgcaaggtca tggcggtccg gtgatcctcg
    49141 acctgctgtt aaaacgcatt ctgaccattc ccggcctgcg gattgctcgc cctggtgagt
    49201 tttccgaacg cgcgtttctt aacgataaac ttgacttagc ccaggccgag gcgattgccg
    49261 atcttatcga cgccagttcg gaacaggcgg cccgttcggc acttaactcg ctgcaaggcg
    49321 cattctccgc acgggttaat catctggtag aagccctcac ccacttgcgc atttacgtcg
    49381 aagcggcaat tgatttccct gatgaagaga tcgatttcct ctccgacggc aaaattgaag
    49441 cccagcttaa tgacgtgatg gccgatctcg atgcagtgcg tgctgaagca cgtcagggta
    49501 gtttgttgcg cgaagggatg aaagtagtga ttgccggacg tcctaatgcc ggtaaatcga
    49561 gcctgttaaa cgcgctggcg ggccgtgaag cggcaatcgt aaccgatatc gccggaacca
    49621 cgcgtgacgt gctgcgtgag catatccaca ttgacggaat gccgctgcat atcatcgaca
    49681 ctgccgggct acgtgaggcc agtgacgaag tggaacgtat tggtatcgag cgcgcgtggc
    49741 aggaaattga acaggccgac cgcgtgctgt ttatggtcga tggcaccacg acagacgccg
    49801 ttgatccggc agagatctgg ccggaattta tcgcccgtct gcccgcgaaa ctgccgatca
    49861 ccgtggtacg caataaagcc gatatcaccg gcgaaacgct gggaatgagt gaagtgaacg
    49921 gtcacgcgtt aattcgtctc tcggcaagga ctggtgaagg cgtggacgtg ctgcgtaacc
    49981 atctcaaaca gagcatgggc tttgacacca atatggaagg cggcttcctg gcgcgccgtc
    50041 gccacttaca ggcgctggaa caggcagcgg agcatttgca gcaaggtaaa gcgcaactgt
    50101 tgggagcctg ggcaggtgaa ctgctggcgg aagagttgcg cctggcacag cagaacttaa
    50161 gcgaaatcac cggggaattt acttcagacg acctgctggg gcggattttc tccagcttct
    50221 gtattggtaa gtaaccgcgc ttacgaagcc gtattctgac tgtcagatgc ggcttcgctt
    50281 cattgttagc actcctgtta tgcctcaacc cttttttaaa cattaaaa
//
LOCUS       DAJTXY010000016        43942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTXY010000016 DAJTXY010000000
VERSION     DAJTXY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.016"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(1144..2826)
                     /gene="glcA"
                     /locus_tag="OO566_001045"
     CDS             complement(1144..2826)
                     /gene="glcA"
                     /locus_tag="OO566_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HCR6604419.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFAGVIGVITLLQAYVFTGMLVS"
     gene            complement(3181..5352)
                     /gene="glcB"
                     /locus_tag="OO566_001046"
     CDS             complement(3181..5352)
                     /gene="glcB"
                     /locus_tag="OO566_001046"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6604420.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGIVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5374..5778)
                     /locus_tag="OO566_001047"
     CDS             complement(5374..5778)
                     /locus_tag="OO566_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HCR6604421.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5783..7006)
                     /gene="glcF"
                     /locus_tag="OO566_001048"
     CDS             complement(5783..7006)
                     /gene="glcF"
                     /locus_tag="OO566_001048"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HCR6604422.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(7017..8069)
                     /gene="glcE"
                     /locus_tag="OO566_001049"
     CDS             complement(7017..8069)
                     /gene="glcE"
                     /locus_tag="OO566_001049"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6604423.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFCYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8069..9568)
                     /gene="glcD"
                     /locus_tag="OO566_001050"
     CDS             complement(8069..9568)
                     /gene="glcD"
                     /locus_tag="OO566_001050"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HCR6604424.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANELGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9814..10584
                     /gene="glcC"
                     /locus_tag="OO566_001051"
     CDS             9814..10584
                     /gene="glcC"
                     /locus_tag="OO566_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR6604425.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10708..11850)
                     /locus_tag="OO566_001052"
                     /pseudo
     CDS             complement(10708..11850)
                     /locus_tag="OO566_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12254..13942
                     /locus_tag="OO566_001053"
                     /pseudo
     CDS             12254..13942
                     /locus_tag="OO566_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            13939..14853
                     /locus_tag="OO566_001054"
     CDS             13939..14853
                     /locus_tag="OO566_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6604426.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14886..15134
                     /locus_tag="OO566_001055"
     CDS             14886..15134
                     /locus_tag="OO566_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6604427.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            15134..16306
                     /locus_tag="OO566_001056"
     CDS             15134..16306
                     /locus_tag="OO566_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6604428.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(16342..17421)
                     /gene="lptG"
                     /locus_tag="OO566_001057"
     CDS             complement(16342..17421)
                     /gene="lptG"
                     /locus_tag="OO566_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6604429.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17418..18488)
                     /gene="lptF"
                     /locus_tag="OO566_001058"
     CDS             complement(17418..18488)
                     /gene="lptF"
                     /locus_tag="OO566_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6604430.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPWQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18519..19079)
                     /locus_tag="OO566_001059"
     CDS             complement(18519..19079)
                     /locus_tag="OO566_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6604431.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLSNE"
     gene            complement(19091..19915)
                     /locus_tag="OO566_001060"
     CDS             complement(19091..19915)
                     /locus_tag="OO566_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604432.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19915..21264)
                     /locus_tag="OO566_001061"
     CDS             complement(19915..21264)
                     /locus_tag="OO566_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6604433.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21310..22068)
                     /locus_tag="OO566_001062"
     CDS             complement(21310..22068)
                     /locus_tag="OO566_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604434.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22100..22813)
                     /locus_tag="OO566_001063"
     CDS             complement(22100..22813)
                     /locus_tag="OO566_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6604435.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22987..23679
                     /locus_tag="OO566_001064"
     CDS             22987..23679
                     /locus_tag="OO566_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6604436.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23728..25227)
                     /gene="pitB"
                     /locus_tag="OO566_001065"
     CDS             complement(23728..25227)
                     /gene="pitB"
                     /locus_tag="OO566_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6604437.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25519..27378)
                     /gene="gss"
                     /locus_tag="OO566_001066"
     CDS             complement(25519..27378)
                     /gene="gss"
                     /locus_tag="OO566_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6604438.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27583..28449
                     /gene="yghU"
                     /locus_tag="OO566_001067"
     CDS             27583..28449
                     /gene="yghU"
                     /locus_tag="OO566_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6604439.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28572..28820)
                     /gene="hybG"
                     /locus_tag="OO566_001068"
     CDS             complement(28572..28820)
                     /gene="hybG"
                     /locus_tag="OO566_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6604440.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28954..29295)
                     /gene="hypA"
                     /locus_tag="OO566_001069"
     CDS             complement(28954..29295)
                     /gene="hypA"
                     /locus_tag="OO566_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6604441.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29288..29776)
                     /gene="hybE"
                     /locus_tag="OO566_001070"
     CDS             complement(29288..29776)
                     /gene="hybE"
                     /locus_tag="OO566_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6604442.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29769..30263)
                     /locus_tag="OO566_001071"
     CDS             complement(29769..30263)
                     /locus_tag="OO566_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6604443.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLSDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30263..31966)
                     /gene="hybC"
                     /locus_tag="OO566_001072"
     CDS             complement(30263..31966)
                     /gene="hybC"
                     /locus_tag="OO566_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6604444.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31963..33141)
                     /gene="hybB"
                     /locus_tag="OO566_001073"
     CDS             complement(31963..33141)
                     /gene="hybB"
                     /locus_tag="OO566_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6604445.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33131..34117)
                     /gene="hybA"
                     /locus_tag="OO566_001074"
     CDS             complement(33131..34117)
                     /gene="hybA"
                     /locus_tag="OO566_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6604446.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34120..35238)
                     /gene="hybO"
                     /locus_tag="OO566_001075"
     CDS             complement(34120..35238)
                     /gene="hybO"
                     /locus_tag="OO566_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6604447.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35427..35714)
                     /locus_tag="OO566_001076"
     CDS             complement(35427..35714)
                     /locus_tag="OO566_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6604448.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35833..36720)
                     /locus_tag="OO566_001077"
     CDS             complement(35833..36720)
                     /locus_tag="OO566_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6604449.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36877..37917
                     /gene="gpr"
                     /locus_tag="OO566_001078"
     CDS             36877..37917
                     /gene="gpr"
                     /locus_tag="OO566_001078"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6604450.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37957..38451)
                     /locus_tag="OO566_001079"
     CDS             complement(37957..38451)
                     /locus_tag="OO566_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6604451.1"
                     /translation="MERFFENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38642..39527
                     /gene="yghA"
                     /locus_tag="OO566_001080"
                     /pseudo
     CDS             38642..39527
                     /gene="yghA"
                     /locus_tag="OO566_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
     gene            complement(39799..40224)
                     /gene="exbD"
                     /locus_tag="OO566_001081"
     CDS             complement(39799..40224)
                     /gene="exbD"
                     /locus_tag="OO566_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6604452.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40231..40965)
                     /gene="exbB"
                     /locus_tag="OO566_001082"
     CDS             complement(40231..40965)
                     /gene="exbB"
                     /locus_tag="OO566_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6604453.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41217..42404
                     /gene="metC"
                     /locus_tag="OO566_001083"
     CDS             41217..42404
                     /gene="metC"
                     /locus_tag="OO566_001083"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6604454.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42544..43203
                     /locus_tag="OO566_001084"
     CDS             42544..43203
                     /locus_tag="OO566_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6604455.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43243..>43942)
                     /gene="yqhC"
                     /locus_tag="OO566_001085"
     CDS             complement(43243..>43942)
                     /gene="yqhC"
                     /locus_tag="OO566_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6604456.1"
                     /translation="FRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQEL
                     LMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREILY
                     YVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFHHN
                     FKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFGVT
                     PGEDAARMRAMQGN"
BASE COUNT        10878 a        11724 c        10822 g        10518 t
ORIGIN      
        1 cggaaaataa gagctttcat taatactgaa ccactttact gttctatttg ttcaggtatt
       61 gcattacggt gtgctgagat ccatcggtgc atccctgtat cccgcgatga gactaaaata
      121 gtcgtgatgt aagctcgtat cagattctta gtgaatacca catttgttat aattaagtga
      181 aaagatacat tgctgattta cattatttgg tttacaaaat gtcatcactt gcagagttat
      241 cgctgacaga tacctttaat ctcttactac tttcgtgtta ttgcaataca accatccgta
      301 aagcagggct atttttagca ggaaatactc aggtgtcaac attcgaactc caactctgaa
      361 atattcatat aaattaatga ttttaatgat tttttcattt cttcgcgcct aataaaagta
      421 ttcaaattca gatgattttt ctgctcaaca gacacaaaca ggcaataata ctctccaaag
      481 cgaacaatat ttggcattac gatttaatgc ttcataatca ccaaattatt taaccataaa
      541 ttttcttatt tctaacaaaa tgattggtgt gttgttggag ttaaaataca tacataatat
      601 taacatctgc caacatttca ctttccgcaa gacggatgat ttcgaatcct catccacgtt
      661 accctatccc aaatatcttt attaataggc agtaacgaac actgttatag caagattaac
      721 ttattaacct aacggcatct tttatgtaac aagtggagat aaattgctta aaattgatcc
      781 tcgacaaatc ttaaaagttc tcattattct gcaaaatcag ctttgcgaat aatcttatgt
      841 ttttgggagt tttgggaatt ttgatctact cattgttcta gtgaaaaata taaataagag
      901 aaaaaacgcc attcgtcatg aaagcaacag atgaagcaat gtaataacat actaaaattg
      961 atctcatgaa agtgggtact ttataaccag cacagaagcc aagttgtata aatattaaac
     1021 aattcgcatc gtgaaacgga gactacgtat aaagaagcca atcgtaaaaa cggttccctg
     1081 gcgagataaa cggcagacaa atggaaagcc gggtgatatt taccttcatc cggctttatt
     1141 gatttacgag actaacatcc cggtaaacac atacgcctgc agcagggtga taacgccgat
     1201 aacgccggca aaaatcagac tgtgcttcac ggtgtagcgg aacagttcag attctcggcc
     1261 caccatgccc gtcgcggcgc aggccacggc gatagattgc ggggagatca tcttgccagt
     1321 tacgccgccg ctggtatttg ctgccaccag cagagtgtca gagacgttga tttgctgcgc
     1381 cgtggtcgat tgcagtgaac caaacagggc gtttgaagaa gtgtccgacc cggtaaggaa
     1441 tacgcccagc cagccgagaa acggtgagaa aaacgggaac atcacgcctg tacctgccag
     1501 taccagcgcc agcgtggtgg acatgccgga atagttggtg acgaaggcga atgccagcac
     1561 catgccaatc gacaatatcg gccacttcag actaattagc gtttcggcaa agacgccaat
     1621 ccctttcttg atgcccacgc cgaggatgaa gatagagata atcgccgcaa taaaaatagc
     1681 ggtgccgcca gccgagagag gttcgaattt aaacaccgca tccattggcg ttggttgggc
     1741 gacaatgggt gccgctttca acacttgttg atgcaaatga gggatctgga aattaatcac
     1801 cagtgaataa aacgcgccgc ccggagcaaa taacgcttta aacggcttca tggtccagat
     1861 ggtgaccagc accgttaaga ttaaaaacgg tgaccacgca cgaatgattt gccccagact
     1921 atattctgaa ggcacgggac cgccagaaga tggcttattt accaccatcg cacctgcgga
     1981 ttgccccatg ctgattgccg tttcggtatt tttcggccgc cagactttaa ggaataaagc
     2041 gagtgagacg atactcacca gcgccgaagt aatatccggc agttccggac caatatagtt
     2101 agaggtaaag aactgagtga cagcgaagct tcccccagca accagcgccg ctggccacgt
     2161 ctctttcacc cctttccagc cgtccatcat tgctaccagc cagaacggca caagaaccga
     2221 caggaacggt aactgacgtc ccgccattgc gccaatgtgg aacggatcga ttcccgttac
     2281 ctgaccggcg accagaatcg gcacgcccaa cgcaccaaac gccaccggcg cagtattggc
     2341 aatcagacac agccccgccg cgtataacgg tttgaagccc aggcccacca gcagcgcacc
     2401 ggtaatcgcc accggcgcac caaagccagc cgctccttcc agcaacgcac caaaggagaa
     2461 accaatcagt aacacctgca aacgctgatc gtcggtgatg gagataaccg agctgcggat
     2521 aatatcgaac tgcccgctgg caacggttaa tttatacagg aacaccgccg cgacaataat
     2581 ccacgcaatt ggccataatc cataaataaa gccatagccc gcagcagcaa atgccatatc
     2641 aatcggcatt ttaaaggcga atatcgcaat caggatagat aatataaggg ttattgctcc
     2701 agcgacatgt cctttcagac gtaataccgc gagtgcaacg aagaagaata ttatcgggat
     2761 cagggcgacc agagcggata gccccagtcc tcccatcggc atatacattt gggtccaggt
     2821 aaccatattg ttcagtctct tattatcttt atatgcttga tatacttaag gttgtaataa
     2881 gcaaaagagg actgaactgt aaaatatagg cgttatactt tacagcaaca gtacgccgct
     2941 aacgcaattg ctacctctgg cataacaagt atatcgggta agggtttctg ttccgcacac
     3001 gcaggcgcag agtatcgtta agatgtccat attgttgttt taggcccgct agtaatgcgc
     3061 tacgggtatt taatattgtt aaaccctgat aatcgctccg gttatttccg ggataaatgt
     3121 actaccgcag ttactatcat agccccgaca ataaaacttg ccggggcttt tttgacgcta
     3181 ttaatgactt tctttttcgc gtaaacgcca ggcgtgtaat aacggttcgg tataaccgtt
     3241 tggctgtttc acgccgagga agattaaatc gctggcagct ttaaaagcac acgagttagc
     3301 gaaattcccc gccatcggac gataagccgg atcgccagca ttttgctgat caaccacttt
     3361 cgccatattc tccagcgacg cctgcacctg ttctttggtc agaataccgt gacgtaacca
     3421 gttggcgata tgctggctgg agatacgcag cgttgcgcgg tcttccatca gcgccacgtt
     3481 gtgaatatcc ggcacttttg aacaaccaat cccctgctcc acccaacgca ccacgtaccc
     3541 cagaatcccc tgcacgttgt tatccagctc ttgttggatc tcttgcgccg accagttagc
     3601 gttttcagcc accggaatag tcagcagatc gtccagcagc ggttcaaatt cagcattgaa
     3661 ctcggtctgg gcaatgttgg cttgcacgct ctgtacgttg gtttggtggt agtgcagcgc
     3721 atggagcgta gcagcggttg gtgacggaac ccaggctgtg tttgccccgg cacgcagttg
     3781 gtcgcccttc tggctgtaca tgtctgccat caggtccggc attgcccaca tgcctttacc
     3841 gatttgcgct ttaccgcgca gcccacagaa cagaccggaa agcacgttat tacgttcgta
     3901 ggctttgatc cacggcgtcg atttcatctg gtttttacgc agcatcgggc cagcttccat
     3961 caccgaatgc atttcatcgc cggtacggtc gaggaaaccg gtattgatga acgccacgcg
     4021 gttgcgcgcc tgagcgatac agctccgcaa gttcagcgag gtccggcgtt cttcatccat
     4081 aatgcccatt ttcagggtat tcggtactat accgagcatt gtctcaatgc gggtaaacag
     4141 tttgttggcg aacgccactt cctgcggacc gtgcattttc ggtttcacaa tatagacgct
     4201 gccagtgcgc gagtttttct gcacttttaa atcatagagg gcaatcgcgc cagtcatgac
     4261 gccatcaaga ataccttccg ggatttcatt gccttcgctg tcccaaatca caggaatggt
     4321 catcaaatga cccacgttgc ggataaacag caacgagcgt ccgtgcagag aaatttcaga
     4381 gccatcggcg gcggtgtact gacgatcgtc attcagttta cgtacgattt gccgaccgtt
     4441 tttctccatt ttctcttgca gagtcccctg catcaggccc agcagattgc ggtacagcag
     4501 gattttatct tccgcatcaa ccgccgcgac cgaatcttcg cagtcgagaa tggtactgat
     4561 agcagcttcg acgataacat cgttgatgtg cgccggatcg tctttgccaa tccgcccgtt
     4621 ggcatcaatt tgcagttcaa tatgcaggcc gttatttttc agcaaaatgc aggtcggcgc
     4681 agcggcatcg ccacggtaac cgacaaactg tgctggagta cgtaacgtgg tttctttacc
     4741 atttttcaac tggatgcgta attgtttatc gaccacctta aacgccacca catcctgata
     4801 gctgccgttt tccagcggta gtgattcatc gaggaaacgt cgaacccagg cgataacctg
     4861 ctcaccgcgc tgcggatcgt agccactgac catcgcccct tcctgcggga tgatgtcgct
     4921 gccgtataac gcatcgtaca gtgagcccca gcgagcgttc gccgcgttca gcgcgtagcg
     4981 ggcgttcatt gccggaacca ccagctgtgg ccccgcctga ctggtgattt cgctgtcaat
     5041 gcccgtggtt tccaccgtca cgcgctccgg ttgcggcacc aggtagccca gttcacgaag
     5101 gaaagattta taggccgctt tatcttttac cggccccgga ttgctgcgat gccactcatc
     5161 aagcgccgcc tgaatgcgat cgcgttctgc cagcaactga cgattttctg gtgccagatc
     5221 gtgaacgatc tcatcaacat tgtgccagaa tgcagcagcg tccagttcca cgcccggcaa
     5281 aacttctcca tccacaaaac gtttaaaatt ggcgtcaatg cgtaaacggc cctgggttat
     5341 ggtttgactc attgtttctc tcctcgtttt cgcttatttc gccaacaccg ctgctgccgc
     5401 tttcgcgacc tgcgcatcct gtgctccggt taaaccagaa acgcccacgg caccaataat
     5461 ttgcccatca acaacaaccg gtacgccgcc ttccagcgac gtcagtaacg gcgcagtcac
     5521 gaacgcggta cgtccgttgt tcaccatctc ttcatagccc ttagtttcac gacgccccag
     5581 cgcggcggta cgcgctttct cctgggagat gtaagccgca atcggcgcgc aatcgtccat
     5641 gcgacttaac gccagcagat gaccgccgtc atcggcaaca gcaatggaaa cagaccagtt
     5701 atttttctgc gcctcttcct gacctgcggc aataattgca ctcgccattt gctggctaag
     5761 aatgacttta gttttcattt ttttattcct tttcaagggc ttgttctaca atttcaatcc
     5821 agtgacgcac agaggtacga ccggcgctcg ccagatgcgt ctggcaacca atgttggcgg
     5881 tgacgatcat ttccggtttg ccgctttcca gcgcattcat tttgttatcc cgcagctggc
     5941 gtgccagatc gggatgcgtt aacgcatatg ttcccgctga accgcagcac agatggctgt
     6001 cgggaacgtc cgttaaggta aatccaagac gaagtaacac gttttccact tcgccgttca
     6061 gcttttgcgc atgttgtagg gtacacgggc agtggaaggc cagtttttta tcgccgcgaa
     6121 ttgccagttt ttccagcggt tcctcgcgca gaagttcgac taaatcgacc gccagttcac
     6181 tgacctgacg cgctttatcg gcatataacg catcgttttt cagcatctgc ccatactctt
     6241 tgacgaacgc gccgcaaccg ctggcggttt gcaaaattgc ctcggcacct gcttcaatcg
     6301 cgggccacca ggcatcaata ttattgcgcg cccgtgccaa ccctttctcc tgcgcattaa
     6361 gatgatagtc caccgcgcca caacagcctg cttcgttagc tgacatgacg ctgatcccca
     6421 gacgatccag cactcgcgca gttgccgcgt tggtgttggg cgaaagcgta ggctgggcgc
     6481 agccttccag cattaaaacc cgacgcttat ggcgcagcgg cggacgcggt ttagctttca
     6541 ccgtttcagc aggcagtttt gctctgacct gttccggtaa aaacggtcgc agcaccagcc
     6601 ctacctgcgt cagcgcacgg aagaccgccg gacgcggcac tacctggcgc aatccttcgc
     6661 gcagcattcg ctccggcagt gggcgtttca ctttctgctc gacaatatca cgcccgatat
     6721 ccagcaaatt gtgatagcgc acaccagaag gacaggtggt ttcacaattg cggcaagtga
     6781 ggcagcgatc gagatgctcc tgtgttttaa gcgtgacttc gttgccttcc agcacctgtt
     6841 taatcagata gatgcgcccg cgcggcccgt ccagttcatc gcccagaagc tgataggttg
     6901 ggcaggttgc ggtacaaaat ccgcagtgga cacaggcgcg caggatgctg tcggcttcca
     6961 gcgcgcgcgc gttctgccgc atctcttcgg ttaatcgggt ttgcatagcc tgctcctcaa
     7021 agttccgcgt acatgcgacc ggggttaaac acgccgcaag ggtcgagctg ctgtttaagc
     7081 tgctggtgat agcagaataa aggagccgat agcggggcaa agccaccatc tccggcacta
     7141 aagcgggtcg catgaccgcc agcgttgcgg gcgatgcgat ggatttgatt gtcatcggct
     7201 gtcgatttca gccagcgtaa cgccccgccc cagtcgatca gttgctcgcc gggtaaatcc
     7261 atcatcggcg catcgctggg taatgaaatg cgccataagg tacctggtaa cgagaagaac
     7321 ggcagttgtt gttcacgcaa ttgctgccag aactgacccg caacctcttc gccacccagc
     7381 agttcacgcg ctgcttttac cgatccttcg ccgccctcaa ggcggatcca caacgcattg
     7441 tcgaagtaac ataagccact aatgggtaat ggctggagtt gccactcggc gatttcactc
     7501 atggcttctt gcaggctgat ttcccgacgc aggctcagag aggcacgcgg tcgcggtaac
     7561 actttcattg agatttcagt gagcacgcca agacaaccgt agcttccggc cattaaccgt
     7621 gagagatcgt atccggcaac gtttttcatc acttcgccac caaaacgcag atgttttcca
     7681 gtgccggtaa tgatgcgcgt gccgaggaca aaatcgcgga ccgaaccgct ccacgggcga
     7741 cgcggccccg ccagcccgca ggcgaccatc ccgccccagg tggcttcttc accataatgc
     7801 ggcggctcac agggaagcat ttgcccggcg ctctccagcg ccgcttcaat cgccaccagc
     7861 ggcgttccgg cacgagcggt tatcaccagc tcggtcgggt cgtaattaac aatgccgcga
     7921 tgacaacgca catccagcgt ttgcccggtg actgggcgac ctaaaaaggc tttgctattg
     7981 ctgccctgaa tcaccagcgg cgttttatcg ctaattgcct gattcacctg ctccagcagc
     8041 gcctgactgt aatcacactc gcgtagcatc aaaagcgctc cagttcagga aaaggtaaat
     8101 gaccgtgatg cacatgcatg gcaccaaatt cagcacagcg gtgtagcgtg ggaatgtttt
     8161 tcccagggtt cagcaaacca tcggggtcaa acgccgcctt gaccgcatgg aaggtcgtga
     8221 tttcatcgct gttgaactgg gcgcacattt gattgatttt ttctcgcccg atgccatgtt
     8281 ccccactgat gctgccgcca acttcaacgc agagttcgag gatcttcccg cccagctctt
     8341 ccgcgcgggc aaattcacca agttcgttgg catcgaaaag gattaacggg tgcatgttgc
     8401 catctccggc atgaaagacg ttggcaacac gtaaatcata ttgctgcgat aaacgggcaa
     8461 tgccttccag tacgccaggc agggcgcgac gcgggatggt gccatccatg cagtagtaat
     8521 ccggggagat acgtcctacc gccgggaacg catttttgcg accggcccag aaacgtacgc
     8581 gctctgcttc gtcctgtgcc aaacggacgt cagtcgcgcc cgctttcaac aagatgtagt
     8641 taacccgctc gcagtcttcc tgtacgtcag actccacgcc gtccagttcg cataacaaaa
     8701 tcgcttcggc gtcgacggga taaccggcat gaataaaatc ttccgccgcg cggatcgaca
     8761 ggttatccat catctccagc ccgccgggga taatgccatt ggcgatgatg tcaccaaccg
     8821 caagtccggc tttttctacc gagtcaaagc tggctaacag aacccgcgcc acgggcggct
     8881 tcggcagcag ttttaccgtc acttcggtgg tcacgccgag cataccttcc gatccggtga
     8941 acagcgccag caggtcaaaa ccaggtgaat ccagcgcgtc cgatcccagc gtcagcgcct
     9001 cgccgtccag cgtttgcact tcaattttca gcaggttatg tacggtcaga ccatatttca
     9061 ggcagtggac gccaccggca ttttccgcca cattgccgcc aattgaacag gcgatttgtg
     9121 aggaaggatc cggcgcgtaa tagagattat gcggcgcaac ggcctgggag atcgccaggt
     9181 tacatacgcc cggctgcacg cgcgcgcggc gaccaacggg gttaatgtcg aggctctctt
     9241 taaagcgcgc catcaccaac aacacacctt tttccagcgg cagcgcgcca ccagaaagcc
     9301 cggtgcctgc accacgggtc accaccggta cacgcaggcg atggcagaca gccagaatcg
     9361 ctgtcacctg ttccatttgc ttaggcagaa caaccagtaa tggacgcgtg cgatacgcgc
     9421 tcaacccgtc acactcgtaa ggaatgatct cctcatcggt atgcaggatc tcaagtccag
     9481 ggacatgctc acgcagtgcc atcagtaccg atgtgcggtc gacatcgggt aaagcgccat
     9541 caagacgctc ttcgtacaag atgctcatga gtaggcttcg ctttgttgtg tggcagctga
     9601 tttttgcgcg ctgcttctgt gaacagttat taagcgggct tttcgttttc gtctatctct
     9661 ttagctaccg gtcagaccat tttttttcca gctctgtgac cttgtcttgg ttaacttaat
     9721 gttaaattga tgtaacataa tcacttgcgt gatgtgcgtg ttttgcgagt taagaacaga
     9781 aaaattggtc ctacctgtgc acgaggtccg ggaatgaaag atgaacgtcg ccctatttgc
     9841 gaagtggttg cagagagtat cgaacggtta attatcgacg gcgtactgaa ggtcggtcag
     9901 ccgcttccct cggaacgtcg actgtgtgaa aagctcggct tctcacgctc cgcactgcgt
     9961 gaagggctga ccgtgctgcg cgggcgcggg attattgaaa cggcgcaggg tcgcgattct
    10021 catgtcgcac ggcttaatcg ggtgcaggac accagcccgc tgatccatct gttcagtacg
    10081 cagccgcgaa cgctgtacga tctgctcgac gttcgcgcat tactggaggg cgaatcggca
    10141 aggctggcgg caacgctggg aacgcaggct gattttgttg tgataacccg ctgttatgaa
    10201 aaaatgctcg ccgccagtga gaaccacaaa gagatttcgc tgatcgaaca tgcgcagttg
    10261 gatcacgctt tccatctcgc catttgtcag gcttctcaca atcaggtgct ggtgtttacg
    10321 ctgcaatcat tgaccgatct gatgtttaat tcagtgtttg ccagcgtaaa taatctctac
    10381 catcgaccac agcaaaaaaa gcagatcgat cgccagcatg cgcggatcta caacgcggtg
    10441 ttgcagcggc tgccgcacgt cgctcagcgc gcagcgcgcg atcatgtacg gaccgtgaaa
    10501 aagaatctcc acgatatcga gctggaaggc caccatttga ttcgctcggc ggtgccgctg
    10561 gagatgaaca aggtgggaat gtgagcagag ttttacccta tccccaaggc gaggggaccg
    10621 cccctgcact taacaggatc tggtgttaaa tctgttcatc tataccctct cccgaatgac
    10681 cgcccctgcg ctaaacagga tctggtatta aatttgcttt tcatacaatc ggtaacgctt
    10741 gtacggctcc gccccaatgc gttccagcat gttattcatg cctgtattgg tttcgaggat
    10801 ccattacatc tccagcgcat cgatcttccg gcgggcaaac ggatcgcgta aggcttcaat
    10861 caataacagc gcaatcaccg ggccgatgcg gctgaactga tactcgtcgc gcacgcccat
    10921 cagcggtact cgcgcagttc gcacaccgct gactttcaaa cgccacagca attttgccca
    10981 gccgaaggga aagagcgatc cgttcagatc ggcaatcgcc tcgttgatgt taggcaagcc
    11041 gacaataaac gcgcagggtg cagaatcaat ctcagcgatg tagatcatat cgtccggcac
    11101 cagatattta agttggtcgc ccatggtcgc gaattcatgt tcggtaaacg gcacaaatcc
    11161 ccagttgtgc tgccagccag agttgaaaat ctcacgcagg atctgcatct cttcggcaaa
    11221 ccgctgacga ttgatgcagc gaatggtcac ctttttgcgc acctgatcca tcagtttttt
    11281 tagcgccgga gagaaagtga gatcggttcg ctgcatccac cacgccagta aatcaatgcc
    11341 tttgtgataa cccagttgtt caatatgcgc ggcataccac ggtttgccgt gtggcatcat
    11401 cgcacagggt ggtgtgtcaa aaccttcaat cagtaatccg ctttcctgat tgatattcag
    11461 gctgaaaggc ccgctgatct tacttgcacc ttgtgacttc aaccacgctt ccgctgcgcc
    11521 aaacaacgcg gcaaaatcct gcggatcatc aatggcgtca atcatgccga aatgaccggt
    11581 atcttcaccg taacgctcac ggtgcaaggt atctatttgc gcggtaatac gcccaactat
    11641 ctgccccgct ttttttgcca cccacgcctg ccagatgata tggtccgtcc ccggattttt
    11701 cgcagacaaa tgctcgttgc gttcaatgaa taaagggggt atccagtttg gatcgtcggg
    11761 ataaagtgat gacggaaaag cgataaatgc cttaaggtca tttttattaa ttactttttc
    11821 aattttaatc atcagcagat cgcattccat atctctgtgg ataacaaata attaatggtg
    11881 aaaaacaggt tgcaacagca acgagcggca tcttaataat caactcaatg aatacaaacg
    11941 acgaataaat agtattaatt aatgattatg ataaggtcag aggctacaca gaaacgcctc
    12001 aatgcctttt gtcgatttaa ggcgaccgga caaggcttct tcttaatata ttcataaatt
    12061 aaatatcatt cataataaaa ataaaaaact caaactcatt atttaaatca atgttcattt
    12121 tcacaccgat gaaaatacta tctcatttat cttttatgcc tatataatcc accgcattat
    12181 tgtcatttta tttttgatat ccaatttttt cgtggtgtat atgtctaata aaatctttac
    12241 gcattcccta cctatgcgct atgccgattt tccaacgctg gttgatgctt tggactacgc
    12301 cgctctgagt agagccggaa tgaattttta tgacagacgt tgccaacttg aagatcaact
    12361 ggaatatcag acattaaaaa cgcgtgccga agctggtgcg aagcggttgt tatcgctgaa
    12421 cctgaaaaaa ggcgaacgcg tggcgctgat tgccgaaacg agtagcgggt ttgtagaggc
    12481 tttttttgcc tgtcagtatg ctggcttagt cgccttcccg ttggcgattc caatgggcgt
    12541 cggtcagcgg gattcctgga gcgccaaact ctagggttta ctggcaagtt gccagcccgc
    12601 agccattatc actggtgatg agtagttgcc actggtcaat gccgcgacgc ataacaaccc
    12661 cgaattacat gttttaagcc acgcttggtt taaggcatta ccggaagccg atgttgcgct
    12721 ccagcgtcca gttccgaacg atatcgccta cctccagtac acctccggca gcacccgttt
    12781 tccccgtggc gtcattatca cccatcgcga agtgatggct aatctacgtg ctataagcca
    12841 cgatggcatt aaattacgcc ctagcgaccg ctgcgtctcc tggcagcctt tctaccatga
    12901 tatgggactg gtcggctttc tcctgccccc cgtcgccacg caactttcag tagattattt
    12961 gcgcactcag gattttgcca tgcgtccttt gcaatggctt aaattgatca gtaaaaatcg
    13021 cggcaccgtt tccgttgcgc cgccgtttgg ctatgaattg tgccaacgcc gcgtgaatga
    13081 aaaagatctc gctgaactgg atctttccta ctggcgcgtc gctggtattg gtgctgagcc
    13141 gatctccgca gaacaactcc atcaattcgc tgaatgtttc cgtcaggtta actttgacaa
    13201 taaaactttc atgccgtgct acggactggc agaaaatgcg ctggctgtca gcttctctga
    13261 tgaagcctcc ggggttgtgg ttaacgaagt ggatcgcgac atcctcgaat atcagggtaa
    13321 agccgtcgcg ccgggtgcag agacacgcgc cgtatcgact ttcgtcaact gcggcaaagc
    13381 gttgccggaa catggtattg aaatccgcaa tgaagcaggt atgccggtcg cggaacgtgt
    13441 ggtaggccat atttgcatct ccggtcccag tctgatgagc ggttactttg gcgaccaggt
    13501 ttcgcaagac gagattgccg cgacgggctg gttagacacc ggcgacctcg gttatctgct
    13561 ggacggttat ctgtatgtca ccggacgcat taaagatctg attattattc gtggccgtaa
    13621 tatctggccg caggatattg aatatatagc ggaacaggaa ccggaaattc attctggcga
    13681 tgcgattgct tttgttaccg cccaggaaaa aatcattttg cagatccagt gtcggatcag
    13741 cgacgaagaa cgtcgcgggc agcttatcca cgcgctggcg gcacggatcc aaagcgaatt
    13801 tggcgtgacc gcggctatcg atctgttgcc gccccacagt attccccgaa cgtcctccgg
    13861 caagcctgcc cgtgcggaag cgaaaaaacg ttatcagaag gcttatgctg ccagtcttaa
    13921 tgtgcaggaa tccctggcat gaatcaaact gtcgcggtga cgggcgctac cgggtttatc
    13981 ggtaaatata ttatcgataa cctgctcgcc cgcggctttc acgttcgcgc attgacgcgt
    14041 actgcccgcg ctcacgtcaa tgataatctc acctgggtgc gcggttcgct ggaagatact
    14101 cattcactta gcgagctggt tgccggagcc agcgtagtgg tccattgcgc cgggcaagtg
    14161 cgcgggcaca aagaagagat tttcacccgc tgtaacgttg acggcagcct gcgcctgatg
    14221 caagcagcaa aagagagcgg cttttgccaa cgttttctgt ttatctcttc gctggcggcg
    14281 cgccatcccg agctctcctg gtatgcaaaa tccaaatacg tcgccgagca acgactggca
    14341 gcaatggcgg acgaaattac gctgggcgtt tttcgcccga cagccgtgta tggtcccggc
    14401 gataaagagt taaaaccgct gtttgactgg atgctacgcg gcctgctgcc acgacttggt
    14461 gcgccagata cacaactctc ttttcttcac gtcaccgatt ttgcgcaagc agtgggtcag
    14521 tggttaagcg ccgaaactgt acagacgcaa acctatgaat tatgcgatgg cgtcgccggt
    14581 ggctatgact ggcaacgcgt acgacagctt gccgccaaag cccgttgtgg ttccgtgcga
    14641 atggttgaca ttcctctgcc ggtactcaca ggccttgcgg atatcagtac cgcgttgagt
    14701 cgcctggcag gtaaagaacc tatgctgacc cgctcgaaaa ttcgtgaatt aacccacgcc
    14761 gactggtcgg caagtaataa ccgtatttct gaagatatca attggtttcc cgggattagc
    14821 ctggaacacg cattacgcaa cgggctattt tgattgtctt gttttaaagg tgtgttaacg
    14881 tttctatggt aaatcgtgaa atagtaatgg attacatcct ttcctgttta caggatttgg
    14941 tcgaaaacgg tgtggaaatt aagcccgaca gcgatctcgt caacgatctt ggcctggagt
    15001 cgatcaaagt gatggatctc ttaatgatgc tggaagacag attcgatatt tccattccta
    15061 ttaatattct gctgaatgtc aaaccccctg cgcaattaat ggaaactcta cttccatggc
    15121 tggagaataa ataatggggc tatacgataa atatgcgcgc cttgctggcg agcgtctgca
    15181 atttagtgac aacgggttaa caccgtttgg cacctgtatt gatgaagtgt attccgccac
    15241 cgaaggccgg atcggcaata aaaaagtgat tctggccggg accaataatt atctcggttt
    15301 aacctttaat catgatgcca tcagcgaagg tcaggccgcg ttagcggccc aaggcaccgg
    15361 caccaccggt tctcgtatgg caaacggcag ttatgcgccg cacctggcgt tagagaaaga
    15421 gattgccgag tttttcaatc gccctaccgc tatcgttttt tcaaccggtt ataccgctaa
    15481 tttaggcgtc atcagcgcac ttgccgacca taacgccgtg gtgttactgg atgccgacag
    15541 ccacgccagt atttatgacg cctgttctct cggtggcgcg gaaattattc gtttccgcca
    15601 taacgatgct aaagatctgg aacggcgcat ggttcgcctc ggtgaacggg cgaaagaggc
    15661 gattatcatc gttgagggca tctacagcat gctcggtgac gttgcgccgc tggcggaaat
    15721 tgttgatatc aaacgtcgtc tcggtggtta tttgattgtc gatgaagcgc attcgtttgt
    15781 cgtgttaggc gcaacggggc gcggactggc tgaagctgtc ggcgttgaag atgatgtgga
    15841 tattatcgtt ggtactttca gtaaaagtct ggcctcgatt ggcggttttg ctgtcggttc
    15901 ggaagcgatg gaagtgctgc gctacggcag ccgcccgtat atttttaccg cctctccttc
    15961 tccgtcctgc attgcgaccg tgcgctcgtc gttaagaact attgccacac aaccagagct
    16021 gcggcaaaag ctgatggata acgccaacca tctgtatgac ggtctgcaaa aactgggtta
    16081 tgagttaagt tcacatatta gcccggtagt gccagtaata atcggttcga aagaggaagg
    16141 tttacgcatc tggcgcgagc ttatctcgcc gggcgtgtat gtgaatctta tcctgccgcc
    16201 tgccgcgcct gcaggcatca cgctgctgcg ctgtagcgta aatgctgccc acagtcgtga
    16261 gcaaatcgat gccatcattc aggcatttgc tactctgaag cagtaatgtg agcccgccgt
    16321 ggtgtttgcg ccacggcggg tttaatgttg tctgtcatac caatacacca ggctcaacgc
    16381 caccaacaca aacgctaccg gtaaaccgag cgcggtaaca ggtgcgctta acgcaaatag
    16441 caatcccaga ttaaccatga tttgatagct gatccaggtg agtaagccca cgatgacgcc
    16501 tacagcgaga cggctaccca tcccaggcga acgcggggca ctaaaggtaa acggcacagc
    16561 cagcaaaatc atcgccaggg tcaggatggg ttgccccagt ttttcccaca gagcaaggcg
    16621 gtattcgctg ctgggttgcc cggtttcctg caaataatgg atgtaatgat taagctgacg
    16681 gacagaaaaa gtattccccg gcattgataa ctcttcaaga ttcatactgg tgaaggccga
    16741 ttgccaggca agattatcta atgtttccag cgtttctttg ccattcagcc attttttatg
    16801 attcacacca tgcaggaccc acgttttgtc gtcttcaatg gtggcactgc gtgcataaat
    16861 gtaggattca agcgagagat cgtcgcgata atgaaatatc tccacgccca ccggctggcc
    16921 ctgctcgttc agcgatttca ctgtcacaaa ttcattacca cgcctggccc acagcatatt
    16981 gccggtaatg tcatcgtcct cccccaacgc ggtggcggtc gatttgattt gcaacgcctg
    17041 ttgctgcaat ggcgacgcca cccactcatc tatcgcgcct aacgaaacag tccacaatat
    17101 cccggcaacc agcgccacca gtgcaatacg gaagatagaa aaccccgtgc tgcgaatggc
    17161 ggtaagctca ctgtttttcg ataactgccc cagtccaaca ataccaccca ataacgcgat
    17221 aaacggcgaa agttcgacca gtgtgcgcgg taaggtcatt agcaccacca gcaccgcctg
    17281 agtccagcga taaccgcccg ggctgacacc atccagttcg ttaatcaggt tgaaggtggt
    17341 aaaaagcggc agcaatagcc ctgcagcggc ggcaaaaccg agaaagagat gacggattaa
    17401 atagcgactg aaaacattca tcgggaaaat ttctgcagca aagagaagtc acgtgcgacc
    17461 agtatcagca ggcccattag catgagtccg ggcactaacc acaaaccagg aatgagggga
    17521 atcgcgccgt tagccaccag tgtacggcag acgtcgccgc cataaaaaat ggcaataaac
    17581 agcaacgtca gtggcaataa cgttgaaaat cgcccttgcc acggttttac ccgacttaat
    17641 gaaatggcca gcagcgccat caacaatgcg gtcaggccac ggctttgtcg ccattgcagt
    17701 tcggcatgat cggcaggaaa ggcggagcgc gccagctccg tgaccgattt cgctttgcgt
    17761 ttgacgttcg ggctttgatc cagcggattc agatgtaatt gcagattacg gtagatctgt
    17821 tcgttgtcgt cacgcccctg atgatcgaga agataggcag tgccgttgtg caacataacg
    17881 gtcggtttct cgggtgatgg gtcaaccaca tcaaccgaac gggcgcggaa aatacgggtt
    17941 cgattggcag tagaagtata aatcagcgcg tcagtcaggc gattattatc ctgatcaacc
    18001 gtctgcgaaa ggatcattcg tccgttatcg ttagtgttaa atttctttgc ccgcaactgg
    18061 cgaacatcca gctccgactg cgactgctgc tccagttgat aaatctgcgc ataagcccac
    18121 ggacgaccat acatcgacag aagggtgaca aaaatactta gcggtatcgc cagataaaga
    18181 acggctttgt acaagcgtcc cggactgccc cccgccgcag agatagcggt aatttccgag
    18241 tcggtataca tctgccctag cgtcacgcca actgacacat acagaccaac aggtaacaac
    18301 atctccagtg caatcagtac tttgtagaaa acgatatcca gcacaacatc aagagctaat
    18361 gtcccatttg ccgcttcggt cagataacgc tgtgcggagt agctggcgaa aataaagatc
    18421 agaaaaccga cgataatcag caccagacgg cgcgtaccac gcatgatgta gtgttcaacc
    18481 agcttcatct cactaacgcc ctcaatcctt tgagtttttt attcattgct cagtttctgc
    18541 gccaacgcaa tgacatgatc tacctcttcc ggcgtcaggc tgccgtcctt tgcccactga
    18601 atttgcccgg ctttattcag caccagaatg gttgaactct gctccggcaa tcgccaggca
    18661 acgcgtccct gtccattgcc gtcaataacg aattgcgccc acggatagcg gcgtttattt
    18721 ttttcgattt tgccaacaac aaaatagcca gtaccaaaga tggcatcatc ggtgttcaca
    18781 atggtggtgg gctgaaaacg atcctgcgga aagtttgccg cttcaacagc tttaattaac
    18841 agtgagttct ttttcttcgc cgacttacgc ccggcgatat actgaataat gcggactttt
    18901 cccgctaatt gtgtgctgtt ccacttttgg tagtgaattt cgtcgtcgct attcagaagc
    18961 agctcgccgc cttcctggat ataaacaggg gtaaccgttt tgccagtgac gaaattgtgt
    19021 gcctgcacac ccggggctaa gagcatgata atcaacgcaa taatcaaacg agatgacatc
    19081 attgctccgg ctatagcgga aaacgcgaaa tcagcagctc taagcgctga caaatatcgc
    19141 tatccagcgc caacggtgca taatacgact ggcggttttc catatcatcc gccaccatgt
    19201 cgtcgtgttt atctcccatc aggccattaa tggcggcgag gaaacgggaa atctgtaacg
    19261 cctctttatt ttcaggatca agcgatctta aggtgtcgta ataagaaacc tgctggctga
    19321 tgatttttcg cacatcatga aattccctgc cggttagtcg cggtttggca ataatctctt
    19381 tcagggtatg catttgcttt tgccgatagg caataaaact gcttacgctt tctaactggc
    19441 tatggcgtaa tgaatagttc accaacgacc agaccggaga gcttaaataa atccgtaaca
    19501 ggttgccgta atacgacaca atatttttct taccgttacg aaagccgtcc tgaaaacgcc
    19561 ccagaaaaac ggtcgttttg ccgaataaaa atccggcctg atgcttcttt aaatacagcc
    19621 gttgcgcaaa acgcaggtgc ttgtagcgcg ctcgcatgta tttaaattgt ttgcgctcat
    19681 cagcagtcag ctcatcgcct ttggcttgtt tgcgaactaa acgcagtaat tcctgacgat
    19741 caacgccatt gagccagaat tgcaggcata acgcgtaatt atcatgaatg atttcggtgg
    19801 tacaacttag tgcaatagtc ggcggcaaac taacctgtgc atcaacgaca tcatcttctt
    19861 caatcgcaac aaatagacgc tcgctcgcct caagcgggaa atattttctc atcatcagga
    19921 tttctctccg gctgcgttat aaagcaagga atggcgctga aaaaatgcct ttaaggttgg
    19981 aattaacgag agcgccacgt ccagacattg cccaccgaag taagcaaacg ctgctcccag
    20041 cacaccaaac agatgacaga acatgaacag caatacgatg tagccgattg aggcgatggt
    20101 ttgcgccacg agaaaagcgc gctgttttcc cgccatcaat agcaatgact cctgcgggaa
    20161 gcccagcatc gaaatcacaa ttgcgcccag catcacctga atcaggtcat acgcttcgag
    20221 atacttaacg ccaaacacca gcgaaatgag cggcttgccg acgatcaaca ccgcgagcgc
    20281 caccagaata ccgattccgc ccgccagtaa accggacttc acacctaaca accacggtct
    20341 ggtggtgcgc ggatctaaac gcatcacctc tgggtaaaaa cttttaccca gcaaacccgc
    20401 tggcgttccg gcggcgtcga agaatgtcat ggcgatttta aataacccag cggcagcggg
    20461 tcctaacacg atccccacca acactgtgct gcacgagtta cgcgccgacc agatggagtg
    20521 ggcaatgttg gttgaccaga caaaactcca cgcgccttta atgtgtcggg cagactcaaa
    20581 cagattcaat ttgaaggcgt tatggatatt tctacggcgt aattcgcgcg cggcaaacca
    20641 ccagtacatg gtgccgccaa ccagattcga cacgtaccag gcaataacaa aacccgcaaa
    20701 accaaagtca aaataccagg ctgcgacgct ccccgctgcg cgcagaaaag gtttcgtcgc
    20761 ctgctgtaca gcaatcaaat caaagcgatc taccgcacgc agaatgccgg tcggcgtgga
    20821 ggaagccatt gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc tttggtcatc
    20881 cagacctaat gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc
    20941 gccgctgacg atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt gctgcggatt
    21001 attgttggtc aaggctggcg ttccgtactg aaccaccagc tgccatgtct gaaacttaat
    21061 aaaatcgctg atcgacttgg cgtacgattg cacaatcacc agtacaccaa acatggcggg
    21121 cgtcatccct ttaccggcac acgagagcgc caacagcccc agcaaggcgc tctcgacatt
    21181 actggagcct aaccaggcgc tattgcgaat aatggtgcga aacgcgccat ctgcaaacca
    21241 atgtttgatg ttaaaaccag ccaattcagc ctgaccttat cgttgatagt aaaaaatatc
    21301 ccgccagcct taagttaaac ttcggcggtc agaaacgatg gcaaccagag aaaccgcctt
    21361 ctgtacctgt tccagcactt cgctgtaggg cgctctggaa tcaatctcaa gaatttttgt
    21421 gccgttatag ccaatcgtcg acatgacgcc gattttgtcc tgcagctcgg catactcatg
    21481 gtcaggcttg cgggagatgg cagtatcaat atcaatcccc aaacgaatga ttaattcagg
    21541 acggtattgc gccatttttt ggtataaact ccgttcgcgc tgcgccagaa acatgctgat
    21601 tttcccggtc gcacgttcga cgccgatccc cggtccgtca taataaaagc ccgaaatttc
    21661 agcctgcggg aagcgatcgc tgaccaccag aacgccactt tgcgccagtc gctgaacctt
    21721 gcgtagattc gccattcttc gcagcgagaa gcagtacata atcaccgccg cccatagcgc
    21781 cggagatttg gttttcatgc tttgggtttt cgaggatttg gccgccagtc gccgttccag
    21841 ccagacgcca accaacggca atcgtttgat tttgtctccg tcttcgccgg agagcagccc
    21901 cagatagcgg cgctcggttt gccagtgttg ttgcagcgat ttcaccaggt cggtggtcag
    21961 cgtggattta ccggtgccat cacacccgac caccgcaatc agccccggaa tgtagttggg
    22021 ctgcggcgcg gttgtactat taacagtttg tgtttgtagt gcatccattc gttaaatcat
    22081 cccctatcca ttttggtgat caggaaagcg cggcgtgaat tgcgcgtagt gacgcttgca
    22141 taatttcatc ggctggttgc cgaccatcca gttcaaggat cttggcgcca ttgaatgtca
    22201 gttgcggcgt aacggcgatt ttttcctgta acgctgccag ttggtggtca ggtttacgcg
    22261 caaacgcggt ttgttcatca atgccaagac gaatcaacaa tacgggtaaa taagacgcca
    22321 tccattggta cagcttcaac tcgcgctgcc ttaacatttt tatccaaccg ttaccgcccg
    22381 tggtttttgc caattgcggg ccatcaaagc gaaaacccgg cacttcaacc tgcgggtagc
    22441 ggtcggtgat gagcagatag ccttgctggc ttttacacaa cattttgcga aacttgtacg
    22501 cccgccagca ggaaagcaga tagataacca gcgcagtaat attgccaggc ggtgttgagg
    22561 gcttttcgtg tacatgtgcc gctttacttc gcagataaca cccacaaggt gcgccaataa
    22621 cagggagctg tgaaatccat tcgccaattc gcccggacga ttgcccgaga taaatgtgtt
    22681 ctgttggcat tcttgctgcc agttcattta ccaggcttgc cgtgagggtc gatttacctg
    22741 aaccatcaca tcctacaatg gcaataacac gcactggcgt ggaattaatt attgacatat
    22801 tacgttgatt cacgaaaaac ccggcagtaa ataatgtatt gaatattacg tggtccgtgc
    22861 tcagaatatc cgttcaacct tattgtgaca atggttaatt gtgacattga cctgaattac
    22921 gctttcataa aaacatatta accaaataaa tatttttaat ggatatttaa attaaaggat
    22981 atattcatgc agtcaataac acctccatta attgccgtta ttggtagcga tggttcaggc
    23041 aagtcaacgg tgtgtgaaca tcttattacc gttgtcgaaa aatatggtgc tgccgaaaga
    23101 gttcatttag gaaaacaggc cggaaatgtc ggtcgtgcag tgacaaaatt acctttgatg
    23161 ggaaaatcct tacataaaac aattgaacga aatcaggtga aaacagcaaa aaaattgcct
    23221 ggaccagttc cggcgctggt aattacagcg tttgtcgccc gtcgcttact gcgctttcgt
    23281 catatgcttg cctgtcgtcg tcgcgggtta attgttctaa ccgaccgtta tcctcaggac
    23341 caaattcctg gcgcttacga tggtacggtg ttcccaccta acgttgaagg tggtcgtttt
    23401 gtctcatggc tggcaagcca ggaacgtaaa gcgtttcact ggatggcgag ccataagcct
    23461 gatctggtca tcaaactcaa tgttgacctt gaagttgcct gtgcacgtaa acccgaccat
    23521 aaacgggaat cgctggcgag gaagattgcc ataacgccac agttaacctt tggtggtgca
    23581 caactggttg atatcgatgc caatcagcct ctggaacagg tgttggttga tgcagaaaaa
    23641 gcgattacgg attttatgac cgcgcgtggt tatcactagt caaaaatgga aatgcccgat
    23701 cgccaggacc gggcattttc aggaaggtta aatcaactgc aatgctatcc agtacagccc
    23761 accagaaaga aaaattgccg ccggtaaagt aaacacccac gccatcagga tgctggttac
    23821 cgttttacgc tgtaacccac cgccgtccac caccatcgtc cctgctaccg cagacgagag
    23881 gacgtgtgtt gtggagacag gcatcccaat ataactggca aggccgatag acactgccgc
    23941 cgtcatttgt gccgccatgc cttgcgcata cgtcatgccg cgcttaccaa tcttctcacc
    24001 gatggtcatc gctacacgac gccagccaat catggtgcca atgccgagcg ccagtgctac
    24061 cgccatgatg atccacaccg gagcgtactc aatggtgctt aacatatcgc tgcgcagttt
    24121 cttcagcagg ttctggtctt ctttactgac gcctggcagt ttcgccagct tcgcggaggt
    24181 atcagagatg cacagcataa tgcggcgcag ctggctgcgc tgactcacgc ttaacggctc
    24241 gtaactttcc atattgcctg gcagcatcgt tttaacgcgc gcaatcgcat caaaggtatt
    24301 tgccggatga cagtgaaact ctgttacttg cgtgccatca gtcgatgctg caggcaatgg
    24361 aggttccatg gcaatcaact tctgcggcag ttcaggatgc tgttgcaggt agtgttcgaa
    24421 gttggtaacg gcatcgcggg tacgggtaat ttcatagccg gacgcattca tattgacgac
    24481 gaagccagca ggggcaatcc ccaccagtac cagcattacc aggccgatcc ctttctgccc
    24541 gtcgttcgcg ccgtgcgaaa acgccacgcc tgcagcggaa acaatcagcg caatacgcgt
    24601 ccagaatggc ggtttacgtt tgcccttttt ctttttgcga tcttccggaa tgcggtgaat
    24661 acggtcacgc tttttcgtcc cactccagaa acggcgcagc aggaaaatca gaccacccgc
    24721 aatgaccagg ccgacgatag gggaaacaat cagcgaggag aaaattttgg ttacttcacg
    24781 caggtttaac gcatccatca ccgatgagcc ggttaacagc gcgttggtta aaccgatgcc
    24841 gataatcgca ccaatcaagg tgtgcgaact ggaggccggt aaaccaaaga accacgttcc
    24901 caggttccag ataatcgccg ccagcagcat ggaaaagacc atcgccagac cgtgagttga
    24961 ccccatattc agcaacaaat cggttggtaa catatggaca atggcatagg caacgctaag
    25021 tccgcccaat aacacgccaa aaaagttaaa aaatgccgcc atcaccacag caagttgtgg
    25081 ctgcatggca cgagtataaa taacggttgc caccgcattc gccgtgtcat gaaaaccatt
    25141 gattgcttcg tagaacaaca caaatgccag agcaagtaat aacaaaagcc ccgtatatat
    25201 atcaaggcca acaaataaat ttagcatata ttagattacg ccattttgaa tttacgaacg
    25261 gacgcattat cagtgacttt aacggcatgg gcaaagtgaa atatcatttt ttaacgataa
    25321 aaagagtgtt atttcttgtg tattagacaa taagcattca ttcaaaacct ttagaaataa
    25381 tcaaaacaca ggtattccac tggttaatat attcacatat gaaatgaata aatatttgaa
    25441 tatataaata ttgaatattt tgattaatcc ccgtactaat tattcttcat aatcagtacg
    25501 gggttgctac aacatgtatt actttttcac cacaattaac ggttcaatat cactctcttt
    25561 tttgatgacc agtgattcat caccgcgcaa acacgtccca ccgtagttgc cgccaacggt
    25621 gaaggtacat acctgaatgt atttaccgtc cactttcggc aaacaccaca gttgctgata
    25681 gatgtttttc tgctcggcaa atttaccgct ggttttgtcc agcacctctt catgatggcc
    25741 gaccaggtcg atattgctac cacagcgacc ggcgatcggt ttcactgcgt aacctgtttt
    25801 caccaattca tcattaacag tgaaatcggt atccagcagg taacgatggt gcgggaacag
    25861 cgaccagagg atcggcagaa tcgctttgtt gccggggatc accgtccaca gcggctcaaa
    25921 gaccagcact tccgggcgca gcaatacgtc gataagacgc acttcgtttt gcggatgacc
    25981 ggtacggatt ggcaccgcag caaactcacg gtcgctaact tcacgaatct gatcaaacgc
    26041 ggtttcccac gcccaggttt tccacacgca gttaaccagt cgcccttccc catcaatcag
    26101 ttgcccggca gcatcccagc ccagttcatc cagcccgcgc aagatacgcg tttcaaagcc
    26161 cgcctggtgc agcgcctgct ccataaactg cgcgtgatag ttttcctcga tatctttgtc
    26221 ctgcatgata tggacaaacg gacgtgcacg actgtgtttc caggcaccag ccagttcgtt
    26281 aatcagccct tccgccggat tgaagccgtt gcctttatag ccctgctccg cccaacgttc
    26341 gaggatcaag cccgcttcgg tatgacagga ggcggagtcg gcgttgtatt cgtaaacctt
    26401 caggccacgc tcatccatgc agaaatccat acgaccagtg atcatatggt gacggcgacg
    26461 ctgccaggag agacgcaaac gtggccagag gattttcgga atgtcgaaca gcgccagcag
    26521 gttgtcatct ttcagcacct tgtcggttgc gtgaagatac atcagatgca gctcgttggt
    26581 ggctttaatt agctcctgct cggcactctc ggtaatggtg tagtaatgat acggatcctg
    26641 attgatcacc tgaccgttgg cctgcacata ggcgttttgt agcggatctt tttcatccag
    26701 ccatttaccg tcaaactggc ctttgttttc cagacgcgct ccactgattt tcagcagctc
    26761 gcctgcaatt tccggctgcg gtaagctgta ttcagtatct tccgtctgga tcatccagcc
    26821 cagaatggtg gtgtcatcaa aagtgtcttt aagggtatag cagccgtttt cgaccaccat
    26881 ctccagctcg cgcgtccact gttgcccttg cggcaacggg gaatgaatca cgttctgttc
    26941 cgcaatacgg actttgttgc catgcaattg ggtaatgatg gcgacatggc cagtgtcttt
    27001 aaattcaccg cctttatccc agataagaag cgcacccgcg accggcgcac gcggcgagcc
    27061 gttaggaaat gcctgcaatg gcaggatgtt gtcattaacc acttctcgca ggaagcgcag
    27121 cgagaaaatc tcccacgcca tacccacgtc agtaaagacc acaccgtaat tcagaaagag
    27181 aaaacggcga gcaaattcaa cgcattgcca cttgtggccc atatattcgt cgtcgatata
    27241 gctacggaat acggcgtcat cttcgtattc ctgcggatcg agagaactgt aatctgaaga
    27301 gtagattgct accccgcctg gggcgtagcc caataatgtc ccgaacgggg catcctggct
    27361 ggtcgttcct ttgctcatca ctttaccttt aacaatacaa cctaagcagt tggcgtaatt
    27421 gttgtgctct gattacctgc tcagcgacat taaccggaca gaggtcagac taatcataca
    27481 cctcatcgca gcggctggcg caaagggtta aaaaattcac attctgtaca gcaagtgacc
    27541 tgctacactg cttcaacact accactcaga aggcaactca ctatgacaga caatacttat
    27601 cagcccgcga aagtctggac gtgggataaa tccgctggcg gcgcgttcgc caatatcaat
    27661 cgcccgtttt ctggtccgac gcatgaaaaa acgctgcccg ttggcaaaca tccattgcaa
    27721 ctttattcgc tgggaacgcc gaacggtcag aaagtaacga ttatgcttga ggagctactg
    27781 gcgctgggcg ttactggtgc agagtacgac gcctggctga ttcgtattgg cgatggcgat
    27841 caattctcca gcggctttgt cgaagtgaac ccaaactcga agatcccggc gctgcgcgat
    27901 catacgcata atccgccgat ccgcgtgttt gaatctggtt cgatcctgct ttatctggcg
    27961 gagaaatttg gctacttcct gccgcaggat ctggcaaaac ggactgaaac gttgaactgg
    28021 ctgttctggt tacagggtgc cgcgccgttc ctcggcggtg gttttggtca cttttaccat
    28081 tacgcgccgg tgaaaattga gtacgccatc aaccgcttta ccatggaagc caagcgcctg
    28141 ctcgacgtgc tggataagca actggcgcag cataagtttg ttgcgggcga tgagtacacc
    28201 attgcggata tggcgatttg gccgtggttt ggcaacgtgg tgctcggcgg cgtgtatgat
    28261 gccgcagagt ttctggatgc gggaagttat aaacatgtgc aacgctgggc gaaagaagtg
    28321 ggcgaacgtc cggcagtgaa acgtgggcgt attgttaacc gcaccaacgg gccgctgaac
    28381 gagcagttgc atgagcgcca tgacgccagt gattttgaga caaatacaga agataagcgt
    28441 caggggtaag ggttggtgtt cgtcgcagca agccatccag gccggataag gcgttcacgc
    28501 cgcatccggc aatcgtgcac aatgcctgat gcgacgctgc cgcgtcttat caggcctaca
    28561 aaagctaatc atcacgcgct ggtaatgtcg taatccattt ggcgcagtgc gtctaatgtg
    28621 gctttggctt catcttcgtc gatgatgctc atggcaaatc cgacgtgtac cagcacccac
    28681 tggcccagca gatcggcagg gttaccttcg caaatcaggg cgatattcac gtcgcgcttg
    28741 ataccacata cttcaacctg cgcaagctgg tgaatatctt caccgacagc cagcacctgg
    28801 cctggaacgc caatacacat tgttaactcc ggttatttcg attgccggac aaacacgaaa
    28861 aagaaccttt agcgggtgtt ccctctcacc ctaaccctct ccccggaggg gcgaggggcc
    28921 cgtccgtgcg ctttttaggc gttgtcctca gtcttattca acttcaatac ttttgacgat
    28981 cagcgaatcg ccggtatcga cacgcaaccg ctcgccgtga cagagcggac actgcgcatc
    29041 gtgctgatga atctccacca cctggctgca atcccagcac caggcctggg cgggtttata
    29101 gacgatatgt aaatcgcacc cttgcgccac cgttccgtgg cagacaattt caaaactaaa
    29161 acggacggcg ctctcctcaa cgcaggagag cgcgccaatt tccagccaca cggcggtgac
    29221 gcgcttaaca tcgtgctgct ccgcctgccg ttggataatt tcaacggcgc tctggcaaag
    29281 cgacaactca tgcattttcg cccctcctgc ggccaaacag cagggcgcga cgccctgcgt
    29341 gtggtacatc cggattggtg actggcaacg aaaggatcat tcgtgcgcag tcatccgtca
    29401 ggcgttggcc ttcttcaatc gacatgctgt gcgaaagcgg cgacatcagc gaacaggaga
    29461 ggtattgcga aacaccgtcc agttcgccaa cggtaaaggt catggtgcca tacggcagtt
    29521 gcagaccaat tttttcactc actttgcgca gcggccagag ttgatccggg ccggggaaga
    29581 taactgcact cagcatccac ggggtgatca cacaccccgt ccactgacct tcgaacagcg
    29641 taaaatcaga aacatacacc ggcattgaag gatgcagaaa agagagatcg tgcatcgaac
    29701 gccgggcaat ttcttcaaac gctgcctgta cttgcgcctt cggggaggtc tggaaacctg
    29761 ctatctcttc agtcatgaat cgcctcccgt gggatggctt ccacgcctga ttcgcgcagc
    29821 gcagccagaa ccagctcaag cgcaggttca atcattgctt caaccgtcgg cgttaagccg
    29881 atgtgtggct ccagcgattc cgggatcacg ccgaccaggg tcagcttttt cggaaactca
    29941 ccggtgaagc gcagggccga caatacgtcg gccaggccaa gctgatgcgg agagattttg
    30001 ttggtaaaca acgccggaac ttcttcatcc cgcaggatca tcatcgttcc cggcgtgttc
    30061 tttttcgaca caatggcatc cgcaataatc agatgatcgc gatttgccat gtcgctaagc
    30121 agctccattc ccgccgtgcc gccatcgagg atctcaacat aatccggcag aatgtatcgt
    30181 tgctctaacg cttcgacaat ccgcacgccg atggcttcat cggtcagcaa aatattgccg
    30241 acccctaaga ctaaaatacg cattacagaa ccttcactga aaccacttcg ttgccgtcgg
    30301 catccactac gtgtaccgca caggccatgc acgggtcgaa ggagtgaatg gtacgcacca
    30361 cttccagcgg tttattcgga tcggcaaccg gtgtacccac cagcgactgc tcgtaaggac
    30421 cgacgtcatc attgaagtta cgcggaccag agttccaggt tgatggaaca accgcctggt
    30481 agttgctgat gataccgtct ttaataacca tccagtgaga gagcataccg cgcggcgctt
    30541 cgaggaagcc aacacctttg aactcacccg ttgccggaat gttcggcttc acaaaggtgg
    30601 tgtgatcgcc tttgccgata ttggtgatca gtgcactgta ttggttttgc aggatatcct
    30661 gcaattcaca gcagtgaacg gtacgaccaa taatacggcc cagcgtggag tgcagctgtg
    30721 ccacttccag cgtgttgcca gtcagtttct gataaatcgc aacgatttca ttcagtttgt
    30781 tctgggtaga ttcgcgacct gccgccagtt tcaccagcat attagccagc ggacccactt
    30841 ctaccgtttt gccgtagaaa gtcggtgatt tcacccagga atatttcccg tcgtcagacc
    30901 aaccatcata agccggaatg gtggtacctt cccacggcgc ctgtggcgct tcgtctttat
    30961 accaggagtg cttcgcgctt tcctgaatgc ctttgatcag gtattcatcg gaatgagaag
    31021 tgatcggacg atacgaggac agatccgcat tctcaatgta gccgcccggg aacaggaagc
    31081 tgccgttttt gctgtcggtc gggaattccg gcacgctcag gtagttcacc gcacctttac
    31141 cgcgcgtcag ccattccggg tagaacgcgg cgataaccgc ggtatcaacc ttataaacct
    31201 gctcaacaaa gtcgctcagt ttgtcgatga aagacttgat gtacatcagg cgctcaaggt
    31261 tcaacacgcc caacccgtcg aggttgattg ggttcgcgac accactcact gccaggttct
    31321 gaatgtgcgg cgttttaccg cccagcagcg ccacgacgcg gttagcgtca cgctggcact
    31381 ccagcgcctg caggtagtgc gctaccgcaa tcaggttcac ttccggcggc agtttcatcg
    31441 ccgggtgacc ccagtagcca ttggcgaaaa tacccaactg accgctggca accagatctt
    31501 tgatcttgtt ctgaacttta gtgaactctt ccggactgtt cagatgccag gtcgaaacgc
    31561 ctttcagcat ttcggaggct ttggttgggt cagcttgcag tgcagaagtg atgtccaccc
    31621 agtccagcgc cgaaagctga tagaaatgaa cgatatggtc atgcgtggtg tgcgcagcca
    31681 gaatgatgtt acggatgtat tgcgcgttaa ccggaacgtc gatattcagc gcactttctg
    31741 ccgcacgaac ggaagacagc gcgtgagtgg tagtacatac gccacagata cgttgcacaa
    31801 tcatccatgc atcgcgcgga tcgcggtttt tcacgatctc ttccatgccg cgccacatgg
    31861 taccggaagc ccatgctttc gaaacgacgc cattttcgat ttcgcaatcg atgcgtaaat
    31921 gcccctcaat acgggttacc ggatcaatag taattctctg gctcatgctt tgctcgcctc
    31981 atgacgatta tgatcgtttt gttttaaagg aggaagtatc ggcagtagac gaatgagtac
    32041 gatgtaagcg caaatctcaa tagccacaaa accaatagaa atcaacagtt cttcccaggt
    32101 cgggaagtag gcgtaaccgc cgcccgggtt gaatgccacc aacgaatagg tcagacgcca
    32161 ggttgcacaa cctaacagtg cgctcagtgc tgacaggaac agcatgcggg aatcattacg
    32221 cagcttcgcc acacgcagaa cgaccagcgg gaagagcatc agcaggactt caatccagaa
    32281 catcacggag tagaagtcac cggcaaacgc taacgacagc ttgtcgcgat agataagctc
    32341 gccaaagcgc agcccgatga aaatcgccag caacacactg atggtgttgg tcagcttgac
    32401 gaacaggctc ttttcatccg gaccgttgcc acgcagaccc gcctgcacca gtgaaccttc
    32461 aaagatgaca atcgagaagc ccatgatgaa cgccgtcagc agcgagaaca gcggcaacat
    32521 ttcatagctc tgccagagcg gatgcacctt gtagcccgcc gagatcatca gcgaccccat
    32581 tgaagactgg tgcatggtcg gcagcagcgc accgagcgcg atgatgaaga acatcacctt
    32641 gttcagacgt tgtagcgaca ccttccagcc cagacgttca aacagtgccg gagcaaactc
    32701 cagagccatc acgccgatgt agatggtcat acagaccgcc gtctcgaaca gtaccgagtt
    32761 cacgttgaag tgacccggaa tgtagaagta cggcaggttc cagtagcgac ccacgtcgat
    32821 agtgatcgac aagccaccca gtgagtaacc aaacagactc gccaacagcg ccggacgcac
    32881 cagcggatgg tattgcccac ggttaaagac gtataccgcc cacgccagcg cccagccgcc
    32941 acaggcaaag ccggtgccaa tcagcaggtc aaacgcgatc cacacgcccc acgggaagcc
    33001 gccgttcagg tcagagacag agcccagacc gaacaccaga cgcttcacaa tcaggagcat
    33061 acagatgacg attaacggtc caaaaatcat gaccggttta ctgatgattt tgccacccag
    33121 cggttgtgga tcatgactca tgatcgtctc ctccgtcgtg atggtcgttt ttggtgttgc
    33181 gacgaaccag cacggttaag cccgccagca cagccagtgg tagcatcatg cctttataca
    33241 gggtgtgttg aatattttcg gaacgcgcac cggtagaaag atcgtccagt ttcggcaggt
    33301 cgagattttc ataaggcaca cccgtcagta ccagaacctg agtaccgccg ccctctttct
    33361 cgccgtacag atgcggataa tatttcggca ccgtatgcag gtaagtgtcg ccagatttca
    33421 gcgtctgacg cggatagtgg tattcgctgc caggcttcag cgccagacgt tttttcgcct
    33481 ccgccatcag ctcttcacgc gtaccgaaaa tcaccgcgcc cgccgggcac acttctacgc
    33541 agccaggcag accgccttta tcgagacgtt ccacaccttt ctggttgcac agctcgcact
    33601 tatgcagcgc accaaacggg ttgttgtagt cgtacttcgg cacgttgtac ggacaggcga
    33661 ccatgcagta acggcaaccg gtgcacacgt ctttgtcgta atggacaatg ccggttttcg
    33721 gatctttttt cagtgcagag accgggcaca cagagacaca gttcggatcg acgcagtgca
    33781 tacactgttt cttaatgtac gcgtagccgt tctcctcctg gtctttgttg acccctgtgc
    33841 cgctggtcca cacctgaatg atgttattgg tatacggcga cagtttgtcg ttgttcgacc
    33901 aggtctgttc cccttgcggg ttacgttcag ggaagttgat atcctgacac ttggtgacgc
    33961 aagcctggca gcctacgcac aaggtcgagt cgtacaacat ccccagcgat cccggaattg
    34021 gcgggcggtt ttcagcagcc gcatgactga cagacggcag cgcgcccgtc agcaatgccc
    34081 cgcaggaggc tgctttaata aaattacgtc tgttcacggt tattctcccc gtgagtcagc
    34141 gttatctttc ttttgctgac gacccagttc acgcaccgcc atcacgctga caccggcaac
    34201 cagcccaacc acaccgccga gcaaaccaat agcgcctgca gagacgttgc cgccctcttt
    34261 agcgttaaca tccggtttct gtgaacgcgg agtctgattt tcgacgttgg caagctgatg
    34321 gatgccttta tggaagccga tacctttttc gttacagcca tagcacgggt gaccaatcgc
    34381 caccggccac acaccgccaa catcgcagaa ttgcagcgtt gagcagttgc cgtaagtttc
    34441 tggcccttta cagccgaggt ggtacaggca ccagccttcg cggtggcctt catcaccgaa
    34501 ctctttggca aaacgaccag catcgaagtg cgggcgacgt tcgcagtgtt cgtgaatcag
    34561 acggccatag gcgaaggtcg gacggttttt gtcatccagt ttcggcggtt tgccgtaagt
    34621 gatgatgtgc gcaacggtcg cgaggaagtt gtgcgggttc ggcgggcagc ccggaatatt
    34681 gataacggtt ttgcctggca gaacttcttg caagctgact gcgccagttg ggttaactcc
    34741 agctgcggca acaccgcccc acgcggagca ggaaccaata gcgataatgg ctgctgcgcc
    34801 ttccgccgct ttgcggatgt gatccacaat cggctcaccg gcaaccatgc aataaatacc
    34861 gttatctttt aatgggatgg aaccatccac cactaacaca tactgccctt tgtacttctc
    34921 gagagcgtta tgtttgttct cttcgacctg atgaccgaag gcggcggaaa gcacttcgtg
    34981 atattccaga gagatagtct ccagcacgag gttttctacc gttggatgcg ttgcacgaag
    35041 cagagattcc gtacaaccgg tgcactcctg cgcgccaatc cagataactg gcggacgctg
    35101 cgggttagta accgattcgg ccatctctgc agcggctttg ctacttaacc ccatggtggc
    35161 ggctaatgct gcacaaagct tcatgaaatc acgacggtta atgccgtgag aatggatgag
    35221 ggtgttatct ccagtcattt atagttattc cgttgcgagg acctggcata tattttgcct
    35281 caatcgcaaa atcaataatg cgatcgatgc gccatttacc acacatttat tatggttatc
    35341 gatatcatga tactgcggcg gggcgctaaa acgaagggaa gaagcattag tgtaattaat
    35401 taaagcaaga taatacgtat gtttgatcaa ttttcgaggc gataaccggc cataaaaaag
    35461 cgcaacgtag aacaggaatt attctcacga aagaaatcca ttaccatctc tttatccagt
    35521 ccatagcggc gcgtcagaat acccgcttcc caggcgctaa gctggcgatc tccggtcttt
    35581 tcgtacatcc ggtgtgagta gcctaataca aaaccgcgtt tataatcggc acagaatttt
    35641 gtcacattaa ccgcactgtc ggcatgcgtt gcttttaatc ccgccattaa gcctttacca
    35701 aaatggttat tcatcgttca ccccattcgt tatatatcgc catatatagc gatattttag
    35761 gcaaagaacg acttttgcgc acgctatttt ttcatacttg tttaagacgt aatcccgtaa
    35821 gagataaacc tatcaggaga gatatttatc gaaccatttc agtgtccttt gccaggcaag
    35881 atcggcggca gatttgtcat aacggggcgt ggaatcatta tggaatccgt gattaacccc
    35941 cggatagata taagcctcat aaaccttatt attgtctttc aacgccgcct cgtaaggagg
    36001 ccagccctcg ttgattcggg tgtccagttc cgcgaagtgg agtagtaaag gcgcttcaat
    36061 cttcgccaca tcggcagtgg gtgcctgacg accataaaac ggcaccgcgc aggccagttc
    36121 cggatacgcg acagccgccg cgttcgatac gccaccgcca tagcaaaatc cggtaatacc
    36181 cactttacct gtcgcttgcg gatagcgttg cataaactca attgcggcaa agaaatcatt
    36241 catcagtttg gttggatcaa cctgctgttg cagctcacga cctttatcat catttcccgg
    36301 ataacctcca acggaactta atccgtcagg tgccagggcg atataccccg ccttcgccac
    36361 tcgccgtgcc acatcttcga tatacggatt cagtccacga ttctcatgca ccacccccac
    36421 ggctggcgtt ttgccgctca tttttgcggg tttcaccaga taaccccgta cctcgccgtg
    36481 accatttggc gaaggatacg tgatgtactc agcaacaatc tccgggtcgg taaactctac
    36541 ctgagtcgcc agcgcataat ttggcttgag caaatcaaac aacgctaatg ccgtcatccc
    36601 gcccaccgca tacttcgccg caaggttgag gaactcacgt ttcgaaattt tcccgtgaac
    36661 gtaatagtcg tagtaatcca acaactcttg tgggaaatct ttggcggtta aacgcggcat
    36721 cgttgcattc ctcaactggt gtttttttaa gcaaagcata agcgcgtgtt tttgcccagt
    36781 ttttcgtcac tctgtgagcc agactacggg atacgcgctg gcgaatcgct aaactagaaa
    36841 cattgtttcg aaattgaacg gtggaaagga gaggtcatgg tctggttagc gaatcccgaa
    36901 cgttacgggc agatgcagta ccgctattgc ggaaaaagtg gtttacgcct gcccgcgtta
    36961 tcgctcggtt tatggcacaa tttcggtcac gttaacgcgc tggaatcaca gcgtgcaatc
    37021 ctgcgtaaag cgtttgattt aggcattacg cactttgatt tagccaacaa ttacgggccg
    37081 cctccaggaa gcgcagaaga gaactttggt cgcctgctgc gcgaggattt tgctgcttat
    37141 cgcgatgaac tgattatctc taccaaggct ggctacgata tgtggcccgg cccgtacggc
    37201 tctggcggtt cacgtaaata cctgctcgcc agcctcgacc aaagcctgaa gcgtatgggg
    37261 cttgagtatg tcgatatctt ttactctcat cgcgtcgatg aaaatacgcc gatggaagaa
    37321 accgcctctg cgctggctca tgcggtacaa agcggtaagg cgctgtatgt cgggatctcc
    37381 tcttactcgc cagagcggac gcaaaaaatg gtcgagttgc tgcacgagtg gaaaattccg
    37441 cttttaattc atcaaccttc gtacaattta ctgaaccgct gggtggataa aagcggcctg
    37501 ctggataccc tgcaaaataa cggcgtgggc tgtattgcct ttactcctct ggctcaggga
    37561 ttgctgaccg gaaaatatct caacggcatt ccgcaagatt cacggatgca tcgtgaaggg
    37621 aataaaattc gtggtctgac gccgaaaatg cttaccgaag ccaacctcaa cagcctgcgc
    37681 ttattgaatg aaatggcaca gcagcgtgga caatcaatgg cgcaaatggc gttaagctgg
    37741 ttgctgaaag atgatcgcgt gacgtcggta ttgattggtg ccagccgcgc ggagcaacta
    37801 gaggagaacg tgcaggcgct gaataatctg acatttagca ccgaggagct ggcgcagatt
    37861 gatcagcata tcgccgatgg cgaactgaat ctgtggcagg cgtcgtccga taaatgacct
    37921 gttaataacg ggcgcgcgaa ccgcgcccga atcagatcag tgattatgac gagtcagtcg
    37981 gtcaagatag cccatcacaa atgcagaaag cacaaacgtc agatggataa tgacgtacca
    38041 catcagtttg ttatccggga catttttcgc atccataaag acgcgcagta agtgaatgga
    38101 agaaattgcc acaatcgacg ctgctacttt gtttttcagc gacgttgcgt ccattttccc
    38161 cagccagttc agcttctctt tgttctcgga gatatccagc tgtgagacga aattctcata
    38221 accggaaaac atcaccatca ccagcaaacc gccaaccagc gtcatatcca ccagcgacag
    38281 caacacgagg atcaaatctg attccgccat cgagaagata ttcggcagta cgtgaataat
    38341 ctcctggaag aacttcagcg ccagggcaac taacgccagc gaaaggccaa agtacacggg
    38401 ggcaagcagc cagcgagaag catacattgc attttcaaaa aaacgttcca tacattcctg
    38461 tcgtgtagtt aaaacgggca acagtatatc gcaattgagt aaacagcacg caacagcaaa
    38521 tcaacatata cgttaaacct ttgtcgcata attctgaggt ttagggatct tcctggttcc
    38581 cttatcaacg catcgagagc gtctaccctt aaaggacgtt gaacctgaag ggagaaaaac
    38641 gatgtctcat ttaaaatgac ccgaccacgc agtattacac tggtgaatat cccaaacaga
    38701 aacaaccgac gccaggcatc caggcgaaga tgacaccgat accggattgc ggcgagaaaa
    38761 cctatgttgg tagcgctcgc ctgaaagatc gtaaagcact ggtgacaggg ggcgattccg
    38821 gaataggtcg cgctgccgcc atcgcttacg cgcgagaagg ggctgacgtg gcgatcagtt
    38881 atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa gaatgcggac
    38941 gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa attcgcccgt tcgctggttc
    39001 acgaagcgca caaggcgtta ggcgggctgg atattatggc gctggtcgcc gggaaacagg
    39061 ttgccattcc ggatattgca gacctcacca gcgaacagtt tcaaaagacc tttgccatta
    39121 acgttttcgc gctgttctgg ctaacccagg aagcgatccc cctgctaccg aaaggtgcaa
    39181 gtatcatcac cacttcgtca atccaggcat accagccaag tccgcattta ctggactatg
    39241 cggctacgaa ggcggcgatt ctgaactaca gccgtggcct ggcaaaacag gtcgcggaga
    39301 aaggtattcg ggtgaatatt gtcgcgccag gccctatctg gacggcactg caaatttccg
    39361 gcggacaaac gcaggataag atcccgcagt ttggtcagaa aacgccgatg aagcgtgcgg
    39421 ggcaaccggc ggaactggcc cctgtatatg tttatctggc aagtcaggag tcgagctacg
    39481 tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa aatgcccggt
    39541 tgtgaaaagc aaccgggcat cattgtgaat tcactcttac ctgatgtggc gaaaacgcct
    39601 tatccgccta caaacgcatg aagattcaac atgttgcaat agattttgta ggcctgataa
    39661 gcgtagcgca tcaggcattg agcacctaat gccggatgcg tcgtgaacgc cttatccggc
    39721 ctacaaacgc acgaagattc aacatgttgc aatagatttt gtaggcctga tgagcgtagc
    39781 gtatcaggca attctacttt acttcgcttt ggcggtttct tcgccgacca gacctatctt
    39841 caggtaaccc gcctgatgca gcgtatccat taccttcatc aacgtctcgt aatcgacggt
    39901 tttgtccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg cattcaacgc
    39961 cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt tgtctgcctt
    40021 caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag aagcaggcaa
    40081 gttcaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa tcagcagaac
    40141 caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt tatcgtcgag
    40201 gttttcgtta agatgcattg ccatcggaca ttatcctgcg cgtaattttt gtgcgacacg
    40261 aaccggatgc gcagcggcgc tggcttccag atccaggtca cggctttgca gcaacaatac
    40321 ctgcgctgca acatcaccca gcatcgcttt aaagccgcca atctggcgtg caaatacgtt
    40381 atagataacg accgcaggaa tcgctgccac gaggccgatt gccgttgcta acagagcttc
    40441 tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga taccaataaa
    40501 gctgttcatg atgccccaga ccgtaccaaa cagaccaacg aacggagaaa tcgcgccgat
    40561 ggttgccaga tagccgttac cgcggcccat ttgacgaccc actgcggcga cccgacgctc
    40621 cagacggaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt ctgacagttc
    40681 cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac caaaatcagc
    40741 ggcgatatcg ttggcctggt ttaaggaacg tgcttcagcc agcagttgct gctcgcgctt
    40801 aaggcgacgc ttctgattga agaactctac gctcttactg aagaagattg cccaggtgac
    40861 tacggaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg cgtgctgata
    40921 cataccccag acggaaaggt ccgtctgcat taaattatta cccactgtgt atctccagga
    40981 cgcaagtcac aaaatctgcg cataataata tcaaaacgac gtcgaattga tagtcgttct
    41041 cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta cgcagtaaaa
    41101 aagtcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt caggatgcgc
    41161 ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat cccgacatgg
    41221 cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa tacactcttg
    41281 gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt atttgagagt atggaagcca
    41341 aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg cgcggaacgt
    41401 taacccattt ttccttacaa caagcgatgt gtgaactgga aggtggcgca ggctgcgcgc
    41461 tatttccctg cggggcggca gcggttgcta attcgattct tgcttttgtc gaacagggcg
    41521 atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt agcaaaatcc
    41581 tcagcaaact gggcgtaacg acatcgtggt ttgatccgct gattggtgcc gatatcgtta
    41641 agcatctgca gccaaacact aaaatcgtgt ttctggaatc gccgggttcc atcactatgg
    41701 aagtccacga cgttccggcg attgttgccg ccgtgcgcag tgtggtgccg gatgccatca
    41761 ttatgatcga caacacctgg gcagccggtg tgctgtttaa ggcgctggat tttggcatcg
    41821 atgtttctat tcaggccgcc accaaatatc tggttgggca ttcagatgcg atgattggca
    41881 ctgccgtgtg caatgcccgt tgctgggagc agctacggga aaatgcctat ctgatgggcc
    41941 agatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcaca ttaggtgtgc
    42001 gtttgcgtca acatcatgaa agcagtctga aagtggccga atggctggca gaacatccgc
    42061 aagttgcgcg agttaaccac cctgccctgc ctggcagtaa aggacacgaa ttctggaaac
    42121 gagactttac aggcagcagc gggctatttt cctttgtgct taagaaaaaa ctcaatgatg
    42181 aagagctggc gaactatctg gataacttca gtttattcag catggcctac tcgtggggcg
    42241 ggtatgaatc gttgatcctg gcaaatcaac cagaacatat cgccgccatt cgcccacaag
    42301 gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa gatgtcgacg
    42361 atctgattgc cgatctggac gccggttttg cacggattgt ataacattgc cacttttgga
    42421 caattttgca gacattttat tgtgaaaagt cttaaattgt tgcgtccggg atcaaggcgt
    42481 cccggacgat tcaggagtac aataggcaga taaaagctta aacgctgttc cacaggaaag
    42541 tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt tgccgcgctg
    42601 gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct gtttttagaa
    42661 aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatact ggcaggcgca
    42721 ttgattgccc aaggggttat ggattttctg cctacgattg cgattctgac cgccgcagca
    42781 agtctgggct gctggctaag ttatattcag gggcgctggt tagggaatac caaaacggtg
    42841 aaaggctggc tggcacagct tcctgctaaa tatcaccagc gcgccacctg catgtttgac
    42901 cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg tacgctgctg
    42961 ccaaccatgg cgggaatttc cggtctgcca aaccgccgct tccagttttt caactggtta
    43021 agtggattgc tgtgggtcag cgtggtaacc agttttggct atgccttaag tatgattccg
    43081 ttcgttaaac gccatgaaga tcaggtaatg acgttcctga tgatcctgcc aattgccttg
    43141 ttaaccgctg gcttgttagg cacgctgttt gtggtgatta aaaaaaaata ctgtaacgcc
    43201 tgacgatttt ccccgttccc ggctgctgta ccgggaacgt atttaattcc cctgcatcgc
    43261 ccgcattctt gccgcatctt cccccggcgt cacaccgaag taacgtttaa actcacggct
    43321 aaattgcgat gcgctttcat agccgacgcg catcgctgct gcgctggcct tcatgccgtc
    43381 atggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact gcaacggcga
    43441 ggtgctggtg acagacttaa aattatggtg gaacgccgat acgctcatgt tggcttctgc
    43501 cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaattcgtt tcagcacgcg
    43561 gctaatcaga ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc cgccgcaagg
    43621 tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa tacgcgcatc
    43681 cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg aataaaatct cttctgataa
    43741 cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat cttcgccaat
    43801 gtccatcaac agttcctgta actgcaaaat atcgacattg agacgcaacc ctgccagcgg
    43861 cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca gcagcaggta
    43921 ttcattggca tcataacgaa ac
//
LOCUS       DAJTXY010000017        43267 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTXY010000017 DAJTXY010000000
VERSION     DAJTXY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.017"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..403
                     /locus_tag="OO566_001086"
     CDS             <1..403
                     /locus_tag="OO566_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6604457.1"
                     /translation="GAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWR
                     PKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQ
                     FSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            428..3027
                     /locus_tag="OO566_001087"
                     /pseudo
     CDS             428..3027
                     /locus_tag="OO566_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3018..4088
                     /locus_tag="OO566_001088"
     CDS             3018..4088
                     /locus_tag="OO566_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6604458.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQVIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4100..4642
                     /locus_tag="OO566_001089"
     CDS             4100..4642
                     /locus_tag="OO566_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6604459.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGAGVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIQLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4650..5165
                     /locus_tag="OO566_001090"
     CDS             4650..5165
                     /locus_tag="OO566_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6604460.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTLDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5131..5868
                     /locus_tag="OO566_001091"
     CDS             5131..5868
                     /locus_tag="OO566_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6604461.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5979..6989
                     /gene="pyrD"
                     /locus_tag="OO566_001092"
     CDS             5979..6989
                     /gene="pyrD"
                     /locus_tag="OO566_001092"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6604462.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7163..7705
                     /gene="zapC"
                     /locus_tag="OO566_001093"
     CDS             7163..7705
                     /gene="zapC"
                     /locus_tag="OO566_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6604463.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7702..8811)
                     /gene="ycbX"
                     /locus_tag="OO566_001094"
     CDS             complement(7702..8811)
                     /gene="ycbX"
                     /locus_tag="OO566_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6604464.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9055..11163
                     /gene="rlmKL"
                     /locus_tag="OO566_001095"
     CDS             9055..11163
                     /gene="rlmKL"
                     /locus_tag="OO566_001095"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6604465.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11175..13082
                     /locus_tag="OO566_001096"
                     /pseudo
     CDS             11175..13082
                     /locus_tag="OO566_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13212..14465
                     /gene="pqiA"
                     /locus_tag="OO566_001097"
     CDS             13212..14465
                     /gene="pqiA"
                     /locus_tag="OO566_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6604466.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAVVIFLASIMVPTLKMSAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14470..16110
                     /gene="pqiB"
                     /locus_tag="OO566_001098"
     CDS             14470..16110
                     /gene="pqiB"
                     /locus_tag="OO566_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6604467.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16107..16670
                     /gene="pqiC"
                     /locus_tag="OO566_001099"
     CDS             16107..16670
                     /gene="pqiC"
                     /locus_tag="OO566_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6604468.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16926..17093
                     /gene="rmf"
                     /locus_tag="OO566_001100"
     CDS             16926..17093
                     /gene="rmf"
                     /locus_tag="OO566_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6604469.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17163..17681)
                     /gene="fabA"
                     /locus_tag="OO566_001101"
     CDS             complement(17163..17681)
                     /gene="fabA"
                     /locus_tag="OO566_001101"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6604470.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKIT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17750..19510)
                     /locus_tag="OO566_001102"
     CDS             complement(17750..19510)
                     /locus_tag="OO566_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6604471.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19695..20147
                     /gene="matP"
                     /locus_tag="OO566_001103"
     CDS             19695..20147
                     /gene="matP"
                     /locus_tag="OO566_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6604472.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20223..21269)
                     /gene="ompA"
                     /locus_tag="OO566_001104"
     CDS             complement(20223..21269)
                     /gene="ompA"
                     /locus_tag="OO566_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6604473.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21625..22134)
                     /gene="sulA"
                     /locus_tag="OO566_001105"
     CDS             complement(21625..22134)
                     /gene="sulA"
                     /locus_tag="OO566_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6604474.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22353..22982
                     /gene="sxy"
                     /locus_tag="OO566_001106"
     CDS             22353..22982
                     /gene="sxy"
                     /locus_tag="OO566_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6604475.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22945..25107)
                     /gene="yccS"
                     /locus_tag="OO566_001107"
     CDS             complement(22945..25107)
                     /gene="yccS"
                     /locus_tag="OO566_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6604476.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25117..25563)
                     /locus_tag="OO566_001108"
     CDS             complement(25117..25563)
                     /locus_tag="OO566_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6604477.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25686..27740
                     /gene="helD"
                     /locus_tag="OO566_001109"
     CDS             25686..27740
                     /gene="helD"
                     /locus_tag="OO566_001109"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6604478.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSDVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27772..28230)
                     /gene="mgsA"
                     /locus_tag="OO566_001110"
     CDS             complement(27772..28230)
                     /gene="mgsA"
                     /locus_tag="OO566_001110"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6604479.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28326..28988)
                     /locus_tag="OO566_001111"
     CDS             complement(28326..28988)
                     /locus_tag="OO566_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6604480.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29161..29574
                     /locus_tag="OO566_001112"
     CDS             29161..29574
                     /locus_tag="OO566_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6604481.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29619..29936)
                     /gene="hspQ"
                     /locus_tag="OO566_001113"
     CDS             complement(29619..29936)
                     /gene="hspQ"
                     /locus_tag="OO566_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6604482.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(29994..31184)
                     /gene="rlmI"
                     /locus_tag="OO566_001114"
     CDS             complement(29994..31184)
                     /gene="rlmI"
                     /locus_tag="OO566_001114"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6604483.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31279..31557
                     /gene="yccX"
                     /locus_tag="OO566_001115"
     CDS             31279..31557
                     /gene="yccX"
                     /locus_tag="OO566_001115"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6604484.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31554..31883)
                     /gene="tusE"
                     /locus_tag="OO566_001116"
     CDS             complement(31554..31883)
                     /gene="tusE"
                     /locus_tag="OO566_001116"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6604485.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31974..32633)
                     /gene="yccA"
                     /locus_tag="OO566_001117"
     CDS             complement(31974..32633)
                     /gene="yccA"
                     /locus_tag="OO566_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6604486.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32840..32927)
                     /locus_tag="OO566_001118"
     tRNA            complement(32840..32927)
                     /locus_tag="OO566_001118"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32891..32893),aa:Ser,seq:tga)
     gene            33353..34471
                     /gene="hyaA"
                     /locus_tag="OO566_001119"
     CDS             33353..34471
                     /gene="hyaA"
                     /locus_tag="OO566_001119"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6604487.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLTKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34468..36226
                     /gene="hyaB"
                     /locus_tag="OO566_001120"
                     /pseudo
     CDS             34468..36226
                     /gene="hyaB"
                     /locus_tag="OO566_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36245..36952
                     /gene="hyaC"
                     /locus_tag="OO566_001121"
     CDS             36245..36952
                     /gene="hyaC"
                     /locus_tag="OO566_001121"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6604488.1"
                     /translation="MQQKSDNIVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36949..37536
                     /locus_tag="OO566_001122"
     CDS             36949..37536
                     /locus_tag="OO566_001122"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6604489.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37533..37931
                     /gene="hyaE"
                     /locus_tag="OO566_001123"
     CDS             37533..37931
                     /gene="hyaE"
                     /locus_tag="OO566_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6604490.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37928..38785
                     /locus_tag="OO566_001124"
     CDS             37928..38785
                     /locus_tag="OO566_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6604491.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38919..40463
                     /gene="appC"
                     /locus_tag="OO566_001125"
     CDS             38919..40463
                     /gene="appC"
                     /locus_tag="OO566_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6604492.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40475..41611
                     /gene="appB"
                     /locus_tag="OO566_001126"
     CDS             40475..41611
                     /gene="appB"
                     /locus_tag="OO566_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6604493.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41623..41715
                     /gene="cbdX"
                     /locus_tag="OO566_001127"
     CDS             41623..41715
                     /gene="cbdX"
                     /locus_tag="OO566_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6604494.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41795..43093
                     /gene="appA"
                     /locus_tag="OO566_001128"
     CDS             41795..43093
                     /gene="appA"
                     /locus_tag="OO566_001128"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6604495.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
BASE COUNT        10767 a        10527 c        11520 g        10453 t
ORIGIN      
        1 tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac gggcagtccc
       61 ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa gcgtcatcaa
      121 acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg cgaaactgcg
      181 ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt tcattaccct
      241 gacagacctg aaaataggtg gaaaagtagt taaaaatcaa atgatttccc cctttgataa
      301 ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc gaaccatcaa
      361 tgactacggg gcggaaacgc cgcaactcaa ctgtaaatcg taagccgtct tcagttaaga
      421 gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg gagtgccatc
      481 gtttattggt gggctggtgg tgtttgtgtc ggcagcgttc aatgcacaag ctgaaacctg
      541 gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt ctcgtttcga
      601 aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga atcagacaat
      661 tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga ttgccgcctg
      721 tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc cggcttttaa
      781 acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg ccagcgtaac
      841 gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca tcaaaagtaa
      901 tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc tattactggg
      961 atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt ctggcgacag
     1021 ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc gcaacaattc
     1081 aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca gctatttgca
     1141 gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata ctgcaggcga
     1201 ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca acatgctgcc
     1261 agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca atgcccagat
     1321 aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg gtgcttttga
     1381 aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa tcaaagaagc
     1441 ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac tccagcgtca
     1501 ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta atgaacagca
     1561 ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa cgacatggta
     1621 tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg gatttaacct
     1681 tggtgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc tggcagagca
     1741 aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca accaattggg
     1801 cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca ccctttccga
     1861 caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg aagatacgcc
     1921 gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc aggtcaatat
     1981 ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc aaacttactg
     2041 gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa ttaaagatct
     2101 ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg ctgatcaggt
     2161 gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta atgatagcta
     2221 taccaggaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa cgaagggcac
     2281 attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct gagctacagc
     2341 gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag catggactac
     2401 aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg cagtcaacaa
     2461 caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat taccctgggg
     2521 caatcgctgg gggaaactaa cgtgctgatt gtagcaccag gcgcagagaa tactcgtgtg
     2581 gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc ctatgccact
     2641 tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa cgtggatctt
     2701 gaaaatgcag tagtcaacgt ggttcccacc aaaggggcgt tggttctggc ggagttcaat
     2761 gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc gctgcgtttt
     2821 ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga tgatgatggc
     2881 tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg gggcgaagct
     2941 cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa ctctgcgatt
     3001 acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta ttactacgac
     3061 tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat atcgggcagt
     3121 gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa actctggatg
     3181 aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac gaatcgagtg
     3241 attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga tgggcattcg
     3301 ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat gactatcttg
     3361 ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct ttcgaaaacg
     3421 tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa cgtttaggtg
     3481 gccagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag ccgttcgttg
     3541 gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac aacatacccg
     3601 caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg tctggtacga
     3661 tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt gatctgggcg
     3721 acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc agaacggcag
     3781 aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac gtcaatctga
     3841 gtctgacggc aacctcagac cccagctatc cccaggtgat taagacgtca cgtcctggcg
     3901 tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt gggacactac
     3961 cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat ccagtcagca
     4021 cgacaggcgt accaccagaa accgggcgat ttgaagccac ggcaacggtg agaataaatt
     4081 ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta ttttgttctt
     4141 tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta tgcatattta
     4201 tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac aaaatattca
     4261 tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca ctgctgctga
     4321 tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcgggcg tcctttttag
     4381 cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag gcggaagcgg
     4441 tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc aagaaatcca
     4501 gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac tcaaatatca
     4561 acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg cggttctcta
     4621 ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg attctgttct
     4681 tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc aacattagcg
     4741 gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc aatgtgcagt
     4801 taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag aaatctttca
     4861 tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc ttttcaggaa
     4921 ccctcgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat gcagccagtg
     4981 ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac caggcgagta
     5041 aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa ttgcgacctg
     5101 tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta cttcattatg
     5161 actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct gctggtggcg
     5221 ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc ggcagacaga
     5281 gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat taacagcaaa
     5341 atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc actattactg
     5401 gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg cttgctgaga
     5461 acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag cattgccagc
     5521 gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc ggtatttaaa
     5581 ctgttctggc gacacgaagg tttgccgggc gacccgctgg aagcttatca acaattacgc
     5641 tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta cattaacctg
     5701 attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc gcctaaaagc
     5761 cagcgtcaga caaactggtg tcaggtaata gcaccgtgtc atgtcgcctg gcggacgata
     5821 aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa caggttctga
     5881 aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact cgcacataat
     5941 acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc ttcgttcgta
     6001 aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag caattacgcc
     6061 gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg aaacctgtta
     6121 actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt gataaagacg
     6181 gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc ggtaccgtca
     6241 cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta gatgccgaag
     6301 gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta gagaacgtaa
     6361 aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa gatacgccag
     6421 tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc tatgcgggat
     6481 atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg caatatggtg
     6541 aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg caagcgatgc
     6601 accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa gacgaattga
     6661 tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca accaatacca
     6721 ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt ggcttaagtg
     6781 gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta gaattaaacg
     6841 gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg cgtgaaaaga
     6901 ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa ggtccgccgc
     6961 tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt tataagataa
     7021 tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt atttagacat
     7081 tttgtacttt tattgttgag gttattaagc gaagcaacaa tggattgtgt tgctgcggtt
     7141 ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc gttggtatta
     7201 cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat ttcgctcacg
     7261 ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt gcgttgatga
     7321 tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat ctaaagacca
     7381 aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac cgcaaatgcc
     7441 gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg gcgatgcggc
     7501 ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg aatctggcga
     7561 aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca
     7621 gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga atgttgacag
     7681 cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg gtacacagct
     7741 acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg tgacttcgcc
     7801 ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat aagggatacg
     7861 aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac gaaatgcctg
     7921 tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg cggtatcatc
     7981 agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca cctcatcgcc
     8041 aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga catcgccgtt
     8101 atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg gatgtttttg
     8161 ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa ccacatcaaa
     8221 caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg acgcgccgga
     8281 aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac gttgttggag
     8341 atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa atgacagagg
     8401 tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc gtaattgcac
     8461 ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa ttcgcgcggt
     8521 aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac
     8581 atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag gtgaaggagt
     8641 aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag gttccgtgat
     8701 cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg taagaccaat
     8761 gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca cggtgcggtc
     8821 ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct ataatgcgca
     8881 acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg ccctgggttg
     8941 tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc catgtccaga
     9001 ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga cgatatgaat
     9061 tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga actggaaaat
     9121 ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg cgacacacgg
     9181 cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt gccgctgggc
     9241 gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat caactggaca
     9301 gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa tgacaccatc
     9361 cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc tttcacgcgg
     9421 aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt taacgtctgg
     9481 ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt acatctgcgt
     9541 ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc cgctattgtg
     9601 atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg ttccggtacg
     9661 ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca ccgtgggcgt
     9721 tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt gaaagcggaa
     9781 gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta cggttcggac
     9841 agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg gattggtgaa
     9901 ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc gaaagggccg
     9961 tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga accggcgctg
    10021 attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg ctggaatctc
    10081 tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc agacaaacag
    10141 tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt tgccgaaagc
    10201 acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct gcgtaagaac
    10261 ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg cctgtatgac
    10321 gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt ggtggtgcag
    10381 gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct gttcgatatt
    10441 atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct gaaaacccgt
    10501 gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga gtttcttgaa
    10561 gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga tactggtctg
    10621 ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg caaagatttc
    10681 ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg cggtgcacgc
    10741 agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg caacctgcgt
    10801 ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct ggcgtggctg
    10861 cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt ctctaactca
    10921 aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat gaaagatttg
    10981 aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg tggcttccgt
    11041 atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac gcaaaaaacg
    11101 ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat taccgcagcc
    11161 tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg ttcagcgacg
    11221 cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt tgtctggtgg
    11281 gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa caagggctgg
    11341 atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa caggatccgc
    11401 cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa gaacaagcgg
    11461 aatatctgaa acgctatcac gatatttcgt gcctggtgat gaacgacccg agcgagaaaa
    11521 atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg tggcagctgg
    11581 aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt gcgctgtcgt
    11641 cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg agcaatccgc
    11701 gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc gactggctgg
    11761 aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac cgttcgttta
    11821 tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg acctatccag
    11881 ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa gaactacaaa
    11941 atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgctag gggatcaaag
    12001 cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt cgcgaacgtg
    12061 gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc agccgctccg
    12121 gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag caactggtga
    12181 aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt ccgaatgggt
    12241 gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcgggc agcgggcgta
    12301 ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg gaactggatc
    12361 ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg gttaatggca
    12421 agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa cgggcgatga
    12481 cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg cgtttgttcc
    12541 tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat gtcgaaacgc
    12601 tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg gttagccacg
    12661 atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc ggcggtaaaa
    12721 ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa tatgtggcac
    12781 tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca gaaactgtaa
    12841 aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag ctaccgcaat
    12901 tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg gatgcttcct
    12961 tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct gctgcagagc
    13021 aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa aatggtggct
    13081 gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg cctgatgaac
    13141 tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata gcgcatcatt
    13201 aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc tcgcagtgtg
    13261 acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt ccccggtgtg
    13321 gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc tatgcgttgg
    13381 ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg aacgttgcag
    13441 gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct gaggactacg
    13501 ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt tgtctgataa
    13561 ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag caactggcac
    13621 gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg ggtgtgctgg
    13681 tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc tttctcccct
    13741 ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt cgctggttat
    13801 gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc gtcacgggga
    13861 tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct gatgaacccg
    13921 tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg cagtggacac
    13981 tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg cccatcatgg
    14041 tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc attctgttat
    14101 ggagcgaagg ctcttatccc gtcgctgtgg tgatctttct ggccagtatt atggtgccaa
    14161 cgttaaagat gagcgccatc gcgtggctgt gttgggatgc caaagggcat ggcaagcgcg
    14221 acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc tggtcgatga
    14281 ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt ttaatgagta
    14341 tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca atgttttctg
    14401 ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag catgaggagt
    14461 cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa gaactggtct
    14521 cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct tttttatcat
    14581 tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg aattgaaggt
    14641 ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag cgccacactg
    14701 gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat ggaaaaattg
    14761 ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga agggattagc
    14821 ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc gaaaggcagc
    14881 aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga tgcgaaaggt
    14941 atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga tccggtgctg
    15001 ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca aaaacgcaat
    15061 atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag caatgttcgc
    15121 ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg tgtggagatg
    15181 ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga aggtctggat
    15241 ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca gaagagcatt
    15301 caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga ttcggtacgc
    15361 ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac cgtaagcaaa
    15421 gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg tattccggta
    15481 ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc ggatgttgtt
    15541 gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac cggaaacctg
    15601 gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc aataaccggt
    15661 attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct ggcgcaaatc
    15721 cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa tccgatgatt
    15781 gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct gcaaacgacg
    15841 ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc gacggatatg
    15901 cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg ctccgcagcc
    15961 tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga actgcaaccg
    16021 gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa ggacaaaaaa
    16081 gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt gcagcactgt
    16141 ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta cctgtggtgc
    16201 agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta gagcaggtca
    16261 ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat gtgaagtatg
    16321 tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc aacaccctgg
    16381 ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct ctgggaagcg
    16441 cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat ggcaaggtca
    16501 ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt ccgttccgtc
    16561 tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg gccggtgtct
    16621 ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa ttataaagat
    16681 ttgtaaatat aaccgtctcc ggtatgccgc ctgaggcggt ttttttgttt ctaacgtgcg
    16741 gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta gctcacaaat
    16801 atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta ttgcttaact
    16861 gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta tgagggaaac
    16921 gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt ggttatcagg
    16981 ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat caaaggtcac
    17041 aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc tgattctgtc
    17101 tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct gtctgatgat
    17161 aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc ggtatagatc
    17221 agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg acgattaaca
    17281 atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac ctgaccagtg
    17341 aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag ccagccgagg
    17401 tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat aaccggatcg
    17461 ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc tgcttcaaca
    17521 taccctttgt cgaagttacc acccgtttcg gttattttga ccacacggtc catcatcagc
    17581 atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg accagaggca
    17641 agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt aagccttatt
    17701 ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat cagttcggaa
    17761 taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc tgttgcgatg
    17821 cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg ccatcccaca
    17881 ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag atggtgaatt
    17941 tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga acgttagcag
    18001 tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata gcaaagaagc
    18061 cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga tcgactgaac
    18121 ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc agggcgcaga
    18181 gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca aatgtcagcg
    18241 atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac gcctgcatga
    18301 tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg atgtcggtga
    18361 attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca aaagcgcgtg
    18421 gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt tcgccttcgg
    18481 tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg aggaaacctt
    18541 cgcgccattc acgctgctgc agcattaaat ttagctgctc gccggagaag gtgtcgccat
    18601 cacacaggga ggcgacctct ttacactggc ggaggatcca atgcgggcta agcggaagtg
    18661 tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa gcatccgctc
    18721 ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac cactggctta
    18781 ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata gcctgctctg
    18841 aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct accagaatga
    18901 ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac tcatcaaacg
    18961 caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac agcagaggtt
    19021 gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga tgcaccagac
    19081 caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt tgctccgctt
    19141 ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct gcactcactg
    19201 cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc agttggcctg
    19261 catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca gactcttccg
    19321 gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc tccagcgcaa
    19381 attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc aaatgtggct
    19441 gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat gcaagtttcg
    19501 taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata ccgtaaccgg
    19561 cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa agctcacagg
    19621 atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag ttacacggta
    19681 acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt tggaaatgga
    19741 agtatctggt aaagaagcat cgcgaagggg agttaattac ccgttacata gaagccagtg
    19801 ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg ctggtaaatg
    19861 gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag accattcggg
    19921 ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa tcgatcgatc
    19981 tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt aaaacgcttt
    20041 ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac gcgaataaaa
    20101 tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg acgaaagtca
    20161 gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta ccagacgata
    20221 acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat ctctacgcga
    20281 cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc acaggtgttg
    20341 ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc cgggatacct
    20401 ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc ctggttgtaa
    20461 gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc tttcggatcc
    20521 aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg tttcagggtt
    20581 gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt ctgtacttcc
    20641 ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg gtaggaaaca
    20701 cccaagctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc accgatgttg
    20761 ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta ctcaacacca
    20821 cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc accaggtacg
    20881 ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat gtccagatcg
    20941 tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt gtatgcgccg
    21001 ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat ttcaaaacca
    21061 acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt ttcgtgggtc
    21121 ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag tttagcacca
    21181 gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc agccagtgcc
    21241 actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc caaaatacgc
    21301 catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc tgctcaatat
    21361 taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa gtctacaacg
    21421 tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt cttgtataag
    21481 gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa accgcttcag
    21541 acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg acaaaaaaag
    21601 ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt ttagcccgga
    21661 aagttgtctc gttgtgtgag aggatgcgct taccggacgc ataataaacc ccatagcgtt
    21721 accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat catctgtcaa
    21781 ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg actccactgt
    21841 gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc cagatgcctg
    21901 aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc gcgattgctg
    21961 accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct gatcttcgcg
    22021 atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac gcgcaatttt
    22081 acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt acataatcaa
    22141 tccagcccct gtgagttact gtatggatgt gcagtacatc cagtgacaac aaagatcaac
    22201 cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac ataaattaat
    22261 cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta atgtgaatca
    22321 ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat aagcggatct
    22381 ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca ttgtttggca
    22441 gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt gagttgtatc
    22501 ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc tggctgacat
    22561 ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa agtctatggc
    22621 gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg ctgaaagaga
    22681 aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct tttcatctgg
    22741 aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata cttggggcaa
    22801 aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag attctgttta
    22861 tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca cgccgccagg
    22921 agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg gagcttgagt
    22981 aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc caacttgttg
    23041 tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc gctgtttcag
    23101 gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta acgcgtcatc
    23161 aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta actgctcccg
    23221 gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca gcaagcgaaa
    23281 cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg ataccacaga
    23341 cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg ccagacggtt
    23401 atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt tggcctctgt
    23461 ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcgggccaga tgtagctcac
    23521 tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta acgctacttc
    23581 aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga acatcgttgc
    23641 atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa ccagcagcac
    23701 cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg cgatacctac
    23761 cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt ttggctggca
    23821 gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta tttgaatgat
    23881 ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga gggcggattc
    23941 cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg ggctgtcatc
    24001 agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg attcaattgt
    24061 tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg ttttgagtaa
    24121 atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat gcgtaaaagc
    24181 gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc gtgacagttg
    24241 ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca cgtcgctgtg
    24301 gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac gctcgtgaat
    24361 atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac cacgatcgcc
    24421 acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca gctgaccgtt
    24481 ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag gatcaaacat
    24541 gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca ggttgtcctg
    24601 cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt accagacggc
    24661 accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg ttcccaacat
    24721 agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt gacccagacc
    24781 gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata gccagggcca
    24841 gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg taatgatgag
    24901 gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg ccaccatccc
    24961 cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg ctgtggttcc
    25021 acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccaag tatagcgttt
    25081 gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac gacgtgcatt
    25141 ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa ccggccatag
    25201 tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga ttgaaataca
    25261 ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga aaatcagcca
    25321 gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag ccgggttcag
    25381 ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag tgatctccca
    25441 gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag tcgccaacag
    25501 ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca aaacggtacg
    25561 cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac gtttttttgg
    25621 gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg tggatctaca
    25681 gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac ccttacgatc
    25741 gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa taccttattc
    25801 ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc gagctggaat
    25861 ttgtactgcc ggacgaaaaa gtggtgcgtc tgcacggcac agaatggggc gagacgcagc
    25921 gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg agcgaaattg
    25981 cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga gaaaataaat
    26041 ggctgacgcg tgagcaaacc tctgacgttc agcaacaaat ccgccaggct ttgtcggcgt
    26101 tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg tggcgtaaat
    26161 gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag gcgtataccg
    26221 aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca ccgctgaatc
    26281 cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg gccggtgcag
    26341 gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg cgtggtgaag
    26401 cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa gagatggacg
    26461 agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt catgcgctgg
    26521 cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa ctggaaaatg
    26581 ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc agcgaaaaga
    26641 aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg tcagtgccag
    26701 aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc ctcgatcgct
    26761 gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc agtgcacccg
    26821 aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta ttaaaagcct
    26881 ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt catcaggcga
    26941 ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg gttgatgaat
    27001 ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag caaaacagtc
    27061 agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc agcggtgcgc
    27121 aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc tgtgatttag
    27181 acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt attcagcaga
    27241 acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa aaagctgtca
    27301 cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt tatgccaaac
    27361 cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc agcctggaaa
    27421 aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat gccagcaaag
    27481 ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtgat ggttttccgg
    27541 ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag gatttcccgg
    27601 acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat cgggtatggg
    27661 cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat ctggatgtgc
    27721 cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat attacttcag
    27781 acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt cgttgaaatg
    27841 cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa tgttccatac
    27901 cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat ttagtggatc
    27961 ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa cctgctggtc
    28021 accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc gggaaatcaa
    28081 gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt gatgccgttc
    28141 cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa tatgtttccg
    28201 cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac tttcctacag
    28261 attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc acaggcacaa
    28321 aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg cgtttgtggg
    28381 tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga ctgcggtgga
    28441 atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat catcgtggca
    28501 aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc gacctcataa
    28561 tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg aatcggcgtc
    28621 gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc ttcccgctca
    28681 ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt aaaactgacc
    28741 accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat cgtcttctca
    28801 acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc accgcgcagc
    28861 agagagctgg aaaccttttt gccgtcgagt accagaagat cgacatcggt tgaaagccgc
    28921 agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt cacgatgccg
    28981 gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca aaatttttct
    29041 ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt cattcgtatt
    29101 agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct ggaggaaata
    29161 atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc gctggtgggg
    29221 gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact cgaccagggg
    29281 tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg ccagcagggg
    29341 tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt ccgtaattca
    29401 gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac cctctggatg
    29461 caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg gttaaatgtg
    29521 gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa ataaaaaatc
    29581 cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag acgcggggcc
    29641 tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc aggatgttca
    29701 tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg taggccgtta
    29761 tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt caccgccagc
    29821 tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac cactccgaga
    29881 taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc aatcatagtc
    29941 acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc aagttacatg
    30001 acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat caccggatga
    30061 tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc aattgcggca
    30121 tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga gaaagtcagg
    30181 agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt ataaccacga
    30241 cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat cacgataacg
    30301 tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc atcacggaca
    30361 aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc aatatccagc
    30421 gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag tgccgatacg
    30481 gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac gtagcggcgg
    30541 gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc gtgctgaata
    30601 tccaccagca gcttcatgcc gtgttcttca atcggcagca gggcaggcgg caactcgccg
    30661 gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac atcgctgcga
    30721 tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc cgcgcgctga
    30781 tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg atcgatagta
    30841 atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag gccatctttt
    30901 tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa aaaagcaatg
    30961 tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga agctggcgaa
    31021 taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt ttcaccgagg
    31081 ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg gcgacgaagt
    31141 aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta cttttctgtc
    31201 gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac gcgtccatta
    31261 aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta cgggcgagtt
    31321 cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg cttaaccggg
    31381 tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga agaagggcag
    31441 gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg tgtagaacgg
    31501 gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat ccgttaaata
    31561 cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg gcctttcggg
    31621 aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt cgccatcgct
    31681 tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc acgtacaaaa
    31741 cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc aatcaccact
    31801 gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc cgtttctatc
    31861 tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc ggcagtgtaa
    31921 caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg agattaatcg
    31981 cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag cgaaacatac
    32041 aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt gctggtttca
    32101 aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag atgcagagca
    32161 ggcagttgca ggaagatatt agcaaccata ccaatcagta ccaccacaat acccgccatc
    32221 agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc agagcagcag
    32281 aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat tccggcagac
    32341 agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa ggcgaatgcg
    32401 gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa accatacata
    32461 cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact ggcagttgcg
    32521 gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg cagcacctta
    32581 tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc cattgagtca
    32641 ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag ggcacccaga
    32701 agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat tatcaggtca
    32761 ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg ttagcattta
    32821 gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa ccctttcggg
    32881 tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga atgacgcgca
    32941 ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct gaaaaacagc
    33001 caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac catgaattcc
    33061 ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta aagaaaaata
    33121 ggtccatttt tatcgctaaa agataaatcc acacagtttg tgttgttttg tgcaaaagtt
    33181 tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa ggatgagctt
    33241 tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg tcgcttgccg
    33301 accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg atatgaataa
    33361 cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca gctttctcaa
    33421 atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac caaagattgc
    33481 ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc tggaatgtac
    33541 ctgctgtacc gaatctttta tccgctccgc tcacccactg acgaaggacg tcatcctttc
    33601 cctgatttcc ctctattacg acgataccct gatggctgcc gccggaaccc aggcggaaga
    33661 agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag tagaaggtaa
    33721 tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt ttattgagaa
    33781 actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct gcgcgtcctg
    33841 gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg acaaagtcat
    33901 caccgacaaa cccattatca aagtacctag ctgcccgccg atcccggatg tgatgagcgc
    33961 catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa tgggtcgtcc
    34021 actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc acttcgacgc
    34081 cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt gcctgtacaa
    34141 aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct ggaatgatgg
    34201 cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa atggtttctg
    34261 ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta ctcattccac
    34321 cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg gtgtgcacgc
    34381 agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag aaaccgaaca
    34441 tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca gggatacacc
    34501 atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga aggccacatg
    34561 cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc ctgcggcacc
    34621 atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc gtgggcgttc
    34681 gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt ttacgccatc
    34741 gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa cattatgctg
    34801 acaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg gatggactgg
    34861 atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact ggcgcaaagt
    34921 ctctcctctt ggccgaaatc atcccctagc tatttcttcg acgtacaaaa tcgcctgaag
    34981 aaattcgttg aaggcgggca gttggggatc ttccgcaatg gctactgggg gcacccgcag
    35041 tacaaattgc cgctagaagc caacctgatg ggctttgccc actatctcga agctctcgat
    35101 ttccaacgtg aaattgtcaa aattcacgcg gtctttggcg gtaaaaaccc gcatccaaac
    35161 tggattgtcg gcgggatgcc ttgcgccatt aacattgacg aaagcggcgc ggtcggggcg
    35221 gtcaatatgg aacgcctgaa cctggtgcag tcgattatca cccgcacggc agacttcatt
    35281 aacaacgtga tgatccccga cgccttagcc atcggtcagt tcaacaaacc gtggagcgaa
    35341 atcggcacgg gtctttctga taaatgtgtc ctcagctacg gcgcattccc ggatattgcc
    35401 aacgactttg gtgagaaaag tctgctgatg cctggcggcg cggtgattaa cggcgacttc
    35461 aacaatgtgc tgccagtgga tttggttgat ccgcagcagg tgcaggagtt tgtcgatcac
    35521 gcctggtatc gttatcccaa cgatcaggtc gggcgtcatc cattcgatgg cattaccgac
    35581 ccgtggtaca accccggcga tgtcaaaggc agcgatacca acattcagca gctgaatgaa
    35641 caggaacgct actcgtggat caaagcgcca cgctggcgcg gtaacgcgat ggaagtgggg
    35701 ccgctggcac gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt tgagtcggtc
    35761 gatcgcatga tgtcggcgtt gaacctgccg ctttccggta tccagtcaac gttgggccgc
    35821 aggtaagttg cagtatttct tcgacaagct gatgaccaac ctgaaaaatg gcaatctcgc
    35881 cactgcttcc actgaaaaat gggaacctgc aacctggccg acagagtgcc gtggcgtcgg
    35941 ctttaccgaa gcgccgcgcg gagcgttagg ccactgggcc gccattcgcg atggcaagat
    36001 tgatctctac cagtgcgtgg tgccgaccac ctggaacgcc agcccgcgcg atcccaaagg
    36061 gcagattggc gcttatgaag cggcgctgat gaacaccaaa atggcgatcc ccgagcaacc
    36121 gctggagatc ctgcgtactc tgcacagctt tgatccgtgc ctcgcctgtt caactcatgt
    36181 gctgggcgac gacggtagcg agctgatctc cgtgcaggtg cgttaacagc gaaggagaat
    36241 catcatgcaa cagaaaagcg acaacattgt cagccactat gtctttgaag caccagttcg
    36301 tatctggcac tggctgacgg tgttatgcat ggcggtgttg atggtcaccg gttactttat
    36361 cggcaagccg ctaccttccg tcagtggcga ggcgacgtat ctgttctata tgggttacat
    36421 caggttaatt cacttcagcg ccgggatgat ttttaccgtg gtcttgctga tgcggatcta
    36481 ctgggctttt gttggcaatc gttactcccg cgagctgttt atcgtgccgg tatggcgtaa
    36541 aagctggtgg cagggcgtgt ggtatgaaat ccgctggtat ctgtttctgg caaaacgtcc
    36601 gagtgccgat ataggccata accccatcgc ccaggcggcg atgttcggct atttcctgat
    36661 gtcggtcttt atgatcatca ccggttttgc gctgtacagc gaacacagcc agtacgctat
    36721 ttttgcgccg ttccgttatg tggtggaatt tttctactgg acgggtggca actcaatgga
    36781 cattcacagc tggcatcggc tggggatgtg gctgattggg gcgtttgtga tcggtcatgt
    36841 ctacatggcg ctgcgtgaag acatcatgtc cgacgacacg gtgatctcca ccatggtcaa
    36901 cggctaccgt agccacaaat ttggcaaaat aagtaacaag gagcgttcat gagcgagcaa
    36961 cgcgtggtgg tcatggggct gggcaacctg ctgtgggccg atgaaggctt cggcgtgcgg
    37021 gtggcggaac ggctgtatgc ccattaccac tggcccgagt atgtggagat tgtcgatggc
    37081 ggtactcagg gactgaactt gctggggtat gtcgaaagcg ccagccatct gttgattctc
    37141 gatgccattg actacgggct ggaacctgga aggctgcgaa cctatgccgg agaacgcatt
    37201 ccggcctatc tcagcgcgaa gaaaatgagc ctgcatcaga acagtttctc cgaagtgctg
    37261 gcgctggcag atatccgcgg acatctgcca gcacatattg ccctcgtcgg gctgcaaccc
    37321 gcgatgctcg acgactacgg cggcagcctg agcgaactgg cacgggagca actgcccgct
    37381 gcggaacaat cggcgctggc gcagttggct gcgtggggaa gtgtgccgca accggctaat
    37441 gaatcgcgtt gtctcaatta tgactgtctg tcgatggaaa attacgaagg cgttcgcttg
    37501 cgccagtacc ggatgacaca ggaggagcag ggatgagcaa cgacacgcca tttgatgcgt
    37561 tgtggcaacg aatgctggcg cgcggctgga cgccggtcag tgaatcccgt cttgacgact
    37621 ggcttacgca agcgccagac ggcgtggtgt tattaagcag tgacccgaaa cgcacgccag
    37681 aggtcagcga taatccggta atgattggcg aattactgca cgagtttccc gactatacat
    37741 ggcaggtggc gattgctgac cttgagcaga gcgaagccat cggcgatcgc tttggcgcct
    37801 ttcgctttcc tgccacttta gtgtttaccg gcggaaacta tcgcggcgtg ctgaatggta
    37861 ttcacccgtg ggcggaactg ataaacctga tgcgcgggct tgtcgaaccg cagcaggagc
    37921 gtgcctcatg agcgaaactt ttttccatct gctggggcca ggaacgcaac cgaacgatga
    37981 cagtttcagc atgaatccac tgccgatcac ctgtcaggtg aatgatgaac cgagtatggc
    38041 ggccttggag caatgtgctc acagcccgca ggtgattgcg ctgttaaacg agttacaaca
    38101 tcaactaagc gaacgccagc cgccgttggg cgaggtgctg gcagtcgatc tgttaaatct
    38161 caacgccgac gatcgtcact ttatcaatac gcttctcggg gaaggggaag tgtcagtgcg
    38221 cattcagcag gctgacgaca gtaaaagtga aatacaggag gcgatcttct gcggattatg
    38281 gcgggtgcgc agacgtcgcg gcgaaaagtt gctggaggac aaactggagg ctggctgcgc
    38341 gccgctggcg ttgtggcagg cggcaacgca aaatctcttg ccgacagatt cgctgttacc
    38401 gccgcccatt gatggcctga tgaatggcct accgttggcg catgagttac tggcacatgt
    38461 acgtaacccc gacgcgcagc cgcacagcat taatctgacg caattaccca tcagcgaggc
    38521 tgatcggctt tttctctcac gtctctgtgg gccgggaaat attcagattc gtaccattgg
    38581 ctatggcgag agctatatca actccacggg gttacgccat gtctggcatt tacgctgtac
    38641 ggacacctta aaaggcccgt tactggaaag ttatgaaatc tgcccaatac cggaagtggt
    38701 gctggcagcg ccagaagatt tggtcgactc tgcgcagcgg cttagcgagg tatgtcagtg
    38761 gctggcggaa gctgcaccga cgtaaaaaga cggtaagtat cgctttcagt cttatgaata
    38821 tcgcaatcgg cgaatacctc tggtcgtaga gtttcaggat aaagagggag atctaacatt
    38881 atcgggttat ttttctctct tcgcctacag gagtgcgcat gtgggatgtc attgatttat
    38941 cgcgctggca gtttgctctg accgcgctgt atcacttttt atttgtaccc cttaccctgg
    39001 ggctgatttt tttgctggct atcatggaaa ccatttacgt ggtcaccggc aaaacaatct
    39061 accgcgatat gacgcgcttc tggggtaagc tcttcggtat caattttgct cttggcgtgg
    39121 ctaccggcct gaccatggag tttcagtttg gtactaactg gtcattctat tccaactatg
    39181 tgggcgatat ttttggcgca ccactggcga tggaagcatt aatggccttc ttcctcgaat
    39241 ccacctttgt cgggctgttc ttcttcggct ggcaacggct gaataaatac cagcacctgc
    39301 tggtgacgtg gctggtggcg ttcggttcaa atctctctgc attgtggata ttgaatgcca
    39361 acggttggat gcaatacccg accggtgcgc attttgatat cgacaccctg cgtatggaga
    39421 tgaccagctt cagcgagctg gtctttaacc cggtcagcca ggtgaaattt gtgcacaccg
    39481 tcatggcggg ctacgtgacc ggggccatgt ttattatggc gatcagcgcc tggtatttac
    39541 tgcgcggacg ggagcgcgat gtcgcattac gctcgtttgc catcggttcc gtcttcggta
    39601 ctctggcgat tatcggtacc ctgcaactcg gagacagttc tgcgtatgaa gtcgcgcaag
    39661 tacaaccggt aaaactggcg gcgatggaag gggagtggca aacggaacct gctcctgcac
    39721 cgttccatgt ggttgcctgg ccggaacagg atcaagagcg taacgccttt gccatcaaaa
    39781 ttcccgcgct gctagggatc ctcgccactc actcattaga taaacccgtg ccgggtctga
    39841 agaatttgat ggctgaaacc tacccacgct tgcaacgcgg acgtatggcc tggctgttaa
    39901 tgcaggaaat atctcaaggc aatcgtgagc cgcatgtgtt gcaggcattc cgggagctgg
    39961 aaggcgacct tggttacggc atgttgctct cccgctatgc gccggatatg aatcatgtca
    40021 cagccgcaca gtaccaggcg gcgatgcgtg gcgcgatacc tcaggttgcg ccggtattct
    40081 ggagtttccg catcatggtg ggctgtggtt ccctgctgct actggtgatg ctgattgcgc
    40141 ttgtccagac gctgcgtggc aaaatcgacc agcatcgctg ggtgctgaaa atggcgctct
    40201 ggagtttgcc gttgccgtgg attgcgattg aagccgggtg gtttatgacc gagtttggtc
    40261 gtcagccgtg ggcgatacag gacatcttac cgacatactc cgcgcactcc gctttaacca
    40321 caggacaact ggctttctca ctgatcatga tcgtaggtct ttacaccctg ttcttaatcg
    40381 ccgaagtcta cctgatgcag aaatatgccc gtctggggcc gagcgcgatg cagagtgaac
    40441 aaccgacgca gcaacagggg taaaggagaa aatcatgttt gattatgaaa cattgcgctt
    40501 catctggtgg ctgctgattg gcgtgatcct ggtggtcttt atgatctccg acggatttga
    40561 catggggatc ggctgtttgc tgccgctggt ggcgcgtaat gatgatgaac gccggatagt
    40621 gattaatagc gttggtgcgc actgggaagg caatcaggtc tggttgatcc tcgcaggtgg
    40681 ggcattattt gccgcctggc ccagagtgta tgcagcggcg ttttccggct tttatgtggc
    40741 gatgatcctg gtgctgtgct cgctgttctt ccgtccgctg gcctttgatt atcgcggaaa
    40801 aattgccgat gcacgctggc gtaaaatgtg ggatgccggt ctggtcatcg gcagtctggt
    40861 gccgccggta gtcttcggta tcgccttcgg caacttgttg ctcggcgtgc cgtttgcctt
    40921 cacaccgcaa ttacgcgtgg agtatctcgg cagcttctgg caactgctga cgccattccc
    40981 tttattgtgt ggtttgctca gccttgggat ggtgattttg caaggtggcg tctggttaca
    41041 actgaaaact gttggtgtga ttcatctgcg ttcacagctg gcgactaaac gcgctgcact
    41101 gttggtgatg ctgtgctttt tgctggcagg ttactggctg tgggtcggta ttgatggctt
    41161 tgtactgctc gcccaggatg ctaacggtcc ttccaatccg ttaatgaaac tggtggcagt
    41221 gctacctggt gcctggatga ataatttcgt cgaatcgccc gttttgtgga tcttcccgct
    41281 gctggggttc ttctgcccat tgctgacggt gatggcgatt tatcgtggtc gcccgggttg
    41341 gggatttttg atggcatcat tgatgcaatt tggcgtgatt ttcacggcag gcatcacgct
    41401 gttccccttt gtcatgccgt caagtgtgag tccaatctcc agcctgacgt tgtgggacag
    41461 cacttccagt cagctgacgc tgagcattat gttggtaatc gtgctgatat ttttgcccat
    41521 tgtgttgctc tacactctct ggagctacta caaaatgtgg ggacgcatga caacagaaac
    41581 tctccgccgt aacgaaaacg agttgtacta aggagcagga acatgtggta tttactttgg
    41641 ttcgtcggca ttttgttgat gtgttcgctc tccacccttg tgttggtatg gctggacctg
    41701 cgtctgaaaa gttaacgaac gtaggcctga tgcggcgcat tagcatcgca tcaggcaatc
    41761 aataatgtta gatatgaaaa gcggaaacat atcgatgaaa gcgatcttaa tcccattttt
    41821 atctcttctg attccgttaa ccccgcaatc tgcattcgct cagagtgagc cggagctgaa
    41881 gctggaaagt atggtgattg tcagtcgtca tggtgtgcgt gctccaacca aggccacgca
    41941 actgatgcag gatgtcaccc cagacgcatg gccaacttgg ccggtaaaac tgggttggct
    42001 gacaccgcgc ggtggtgagc taatcgccta tctcggacat taccaacgcc agcgtctggt
    42061 agccgacgga ttgctggcga aaaagggctg cccgcagtct gctcaggtcg cgattattgc
    42121 tgatgtcgac gagcgtaccc gtaaaacagg cgaagccttc gccgccgggc tggcacctga
    42181 ctgtgcaata accgtacata cccaggcaga tacgtccagt cccgatccgt tatttaatcc
    42241 tctaaaaact ggcgtttgcc aactggataa cgcgaacgtg actgacgcga tcctttgcag
    42301 ggcaggaggg tcaattgctg actttaccgg gcatcggcaa acggcgtttc gcgaactgga
    42361 acgggtgctt aatttcccgc aatcaaactt gtgccttaac cgtgagaaac aggacgaaag
    42421 ctgttcatta acgcaggcat taccatcgga actcaaggtg agcgccgaca atgtctcatt
    42481 aaccggtgcg gtaagcctcg catcaatgct gacggagata tttctcctgc aacaggcaca
    42541 aggaatgccg gagccggggt ggggaaggat caccgattca caccagtgga acaccttgct
    42601 aagtttgcat aacgcgcaat tttatttact acaacgcacg ccggaggttg cccgcagtcg
    42661 cgccaccccg ttattggatt tgatcatggc agcgttgacg ccccatccac cgcaaaaaca
    42721 ggcgtatggt gtgacattac ccacttcagt gctgtttatt gccggacacg atactaatct
    42781 ggcaaatctc ggcggcgcac tggagctcaa ctggacgctt ccaggtcagc cggataacac
    42841 gccgccaggt ggtgaactgg tgtttgaacg ctggcgtcgg ctaagcgata acagccagtg
    42901 gattcaggtt tcgctggtct tccagacttt acagcagatg cgtgataaaa cgccgctgtc
    42961 attaaatacg ccgcccggag aggtgaaact gaccctggca ggatgtgaag agcgaaatgc
    43021 gcagggcatg tgttcgttgg caggttttac gcaaatcgtg aatgaagcac gcataccggc
    43081 gtgcagtttg taatgcataa aaaagagcat tcagttacct gaatgctctg aggctgatga
    43141 caaacgaaga actgtctaat gcgtagaccg gaaaaggcgt tcacgccgca tccggtcact
    43201 ttcagtttta ctctttctcg gagtaactat aaccgtaata gttatagccg taactgtaag
    43261 cggtgct
//
LOCUS       DAJTXY010000018        41017 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTXY010000018 DAJTXY010000000
VERSION     DAJTXY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.018"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..222)
                     /locus_tag="OO566_001129"
     CDS             complement(<1..222)
                     /locus_tag="OO566_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HCR6604496.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGI"
     gene            complement(262..1107)
                     /gene="glpF"
                     /locus_tag="OO566_001130"
                     /pseudo
     CDS             complement(262..1107)
                     /gene="glpF"
                     /locus_tag="OO566_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            1533..1778
                     /gene="zapB"
                     /locus_tag="OO566_001131"
     CDS             1533..1778
                     /gene="zapB"
                     /locus_tag="OO566_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6604497.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(1863..2348)
                     /gene="rraA"
                     /locus_tag="OO566_001132"
     CDS             complement(1863..2348)
                     /gene="rraA"
                     /locus_tag="OO566_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6604498.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(2441..3367)
                     /gene="menA"
                     /locus_tag="OO566_001133"
     CDS             complement(2441..3367)
                     /gene="menA"
                     /locus_tag="OO566_001133"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6604499.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(3434..4765)
                     /gene="hslU"
                     /locus_tag="OO566_001134"
     CDS             complement(3434..4765)
                     /gene="hslU"
                     /locus_tag="OO566_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6604500.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(4775..5305)
                     /gene="hslV"
                     /locus_tag="OO566_001135"
     CDS             complement(4775..5305)
                     /gene="hslV"
                     /locus_tag="OO566_001135"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6604501.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(5398..6357)
                     /gene="ftsN"
                     /locus_tag="OO566_001136"
     CDS             complement(5398..6357)
                     /gene="ftsN"
                     /locus_tag="OO566_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6604502.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(6449..7474)
                     /gene="cytR"
                     /locus_tag="OO566_001137"
     CDS             complement(6449..7474)
                     /gene="cytR"
                     /locus_tag="OO566_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6604503.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(7630..9828)
                     /gene="priA"
                     /locus_tag="OO566_001138"
     CDS             complement(7630..9828)
                     /gene="priA"
                     /locus_tag="OO566_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6604504.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            10031..10243
                     /gene="rpmE"
                     /locus_tag="OO566_001139"
     CDS             10031..10243
                     /gene="rpmE"
                     /locus_tag="OO566_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6604505.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(10304..10912)
                     /locus_tag="OO566_001140"
     CDS             complement(10304..10912)
                     /locus_tag="OO566_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6604506.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(11096..11413)
                     /gene="metJ"
                     /locus_tag="OO566_001141"
     CDS             complement(11096..11413)
                     /gene="metJ"
                     /locus_tag="OO566_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6604507.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            11690..12850
                     /gene="metB"
                     /locus_tag="OO566_001142"
     CDS             11690..12850
                     /gene="metB"
                     /locus_tag="OO566_001142"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6604508.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            12853..15285
                     /gene="metL"
                     /locus_tag="OO566_001143"
     CDS             12853..15285
                     /gene="metL"
                     /locus_tag="OO566_001143"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6604509.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            15634..16524
                     /gene="metF"
                     /locus_tag="OO566_001144"
     CDS             15634..16524
                     /gene="metF"
                     /locus_tag="OO566_001144"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6604510.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            16853..19033
                     /gene="katG"
                     /locus_tag="OO566_001145"
     CDS             16853..19033
                     /gene="katG"
                     /locus_tag="OO566_001145"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6604511.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALLVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            19127..20032
                     /gene="yijE"
                     /locus_tag="OO566_001146"
     CDS             19127..20032
                     /gene="yijE"
                     /locus_tag="OO566_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6604512.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(20059..20676)
                     /locus_tag="OO566_001147"
     CDS             complement(20059..20676)
                     /locus_tag="OO566_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6604513.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20951..22043)
                     /gene="gldA"
                     /locus_tag="OO566_001148"
                     /pseudo
     CDS             complement(20951..22043)
                     /gene="gldA"
                     /locus_tag="OO566_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(22054..22716)
                     /gene="fsa"
                     /locus_tag="OO566_001149"
     CDS             complement(22054..22716)
                     /gene="fsa"
                     /locus_tag="OO566_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6604514.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPCQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(22728..25229)
                     /gene="ptsP"
                     /locus_tag="OO566_001150"
     CDS             complement(22728..25229)
                     /gene="ptsP"
                     /locus_tag="OO566_001150"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6604515.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRVRTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            25538..26617
                     /locus_tag="OO566_001151"
     CDS             25538..26617
                     /locus_tag="OO566_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6604516.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            26632..26952
                     /locus_tag="OO566_001152"
     CDS             26632..26952
                     /locus_tag="OO566_001152"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6604517.1"
                     /translation="MTKIIAVTACPSGVAHTYMVAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            27003..29300
                     /locus_tag="OO566_001153"
     CDS             27003..29300
                     /locus_tag="OO566_001153"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6604518.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            29266..30144
                     /locus_tag="OO566_001154"
     CDS             29266..30144
                     /locus_tag="OO566_001154"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6604519.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            30146..30487
                     /locus_tag="OO566_001155"
     CDS             30146..30487
                     /locus_tag="OO566_001155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6604520.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(30474..31325)
                     /locus_tag="OO566_001156"
     CDS             complement(30474..31325)
                     /locus_tag="OO566_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6604521.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(31474..33207)
                     /locus_tag="OO566_001157"
     CDS             complement(31474..33207)
                     /locus_tag="OO566_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6604522.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(33390..36041)
                     /gene="ppc"
                     /locus_tag="OO566_001158"
     CDS             complement(33390..36041)
                     /gene="ppc"
                     /locus_tag="OO566_001158"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6604523.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLDYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(36393..37544)
                     /gene="argE"
                     /locus_tag="OO566_001159"
     CDS             complement(36393..37544)
                     /gene="argE"
                     /locus_tag="OO566_001159"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6604524.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            37698..38702
                     /gene="argC"
                     /locus_tag="OO566_001160"
     CDS             37698..38702
                     /gene="argC"
                     /locus_tag="OO566_001160"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6604525.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            38710..39486
                     /gene="argB"
                     /locus_tag="OO566_001161"
     CDS             38710..39486
                     /gene="argB"
                     /locus_tag="OO566_001161"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6604526.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            39547..40920
                     /gene="argH"
                     /locus_tag="OO566_001162"
     CDS             39547..40920
                     /gene="argH"
                     /locus_tag="OO566_001162"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6604527.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         9339 a        10528 c        11256 g         9894 t
ORIGIN      
        1 aataccgata gctgcaattt gatcggaact gatatcggct ttcgccagca cttctaccag
       61 cgtggagctt tgggtggccc agatttccat tgggtcgtgt tctacccaac ctggttttgg
      121 gtagatttgc tcaaattcgc gctgcgacac gctaatgata ttggcatcgt gatccattac
      181 gaccgcgcgg gagctggtgg tgccctggtc gagcgcaacg atatattttt ttcagtcatg
      241 tttaattgtc ccgtagtcat attacagcga agctttttgt tctgaaggag ttgtggtttc
      301 cttttcttcc acaacacaga tatcgcaagg caaatggcga ccaatcagtt tgcggtaggc
      361 aaatgcacct acaatcgcgc caacgatagg gccgaaaagc ggcaccagga agtaaggaat
      421 gtctctgccg ccggtaaagg cgacattgcc ccagcccgcc agctaggcaa agactttcgg
      481 accgaagtca cgcgctgggt tcatggcaaa acctgtcaat gggcccatag atgcgccaat
      541 gaccgcaatc agtagaccaa tcagcaaggg agccaaaggg ccgcgtggta caccgttgcc
      601 atcgtccgtt aacgccagga taagccccat cagaatagcg gtaatcacca tctcaactgc
      661 gaaagcctgc acaaaattga tatgaggatt agggtaagta gagaaagtgc cagccagatc
      721 aacactttca acgctgccgc gaacaatgtg atgagtctgc tcgaagtcga aaaataaatt
      781 gtagtaaagc ccgtaaacta aagccgcagc gcagaaagcg ccggcaactt gtgaaacgat
      841 aaaaggaata actttgcgct tgtcgaaaca ggcaaacagc cacaatgcaa tggtaacagc
      901 gggattaaga tgcgcgccgg aaacccctgc ggtcaggtag atggccattg ccacccccag
      961 tccccaaatg acactgattt cccactgacc aaaagacgca ccagcgactt ttagtgctgc
     1021 aacgcaaccc acaccgaaaa aaatcaacaa cccggtaccg aggaattcag caatgcactg
     1081 gcctttcaag gttgatgttt gactcataat cggatcctga agagttaatg tttgttgtat
     1141 gtgtgaaagt cacggacctc cacgatgctt gtaggcatgc tgttaactta tcgttaacga
     1201 gcaaaaacga gaaatatcga acttaaaatg tgtgtgcttc gtcataaaat gagcgttatc
     1261 gcgccattta ttccatctga ttgtacatga tgcatgtttg ttagatcatt cgcgcaacaa
     1321 atttattaac attttcgatt gatgtggatt atgtcactag gagaaggatg aaaagtgtgg
     1381 caaaccgtaa tctgcaaggc gtgccgctgg acagcgatgg cgcggcttca tacaatcgga
     1441 gctaactaaa gtgcgctcgt atttattaag gcgtcaccgg taatcgggac gaggattttt
     1501 tatccatcaa cgccttgcaa ttcaggagag gtatgacaat gtcattagaa gtgtttgaga
     1561 aactggaagc aaaagtacag caggcgattg ataccatcac tctgttgcag atggaaatcg
     1621 aagagctgaa agaaaaaaac aactcactgt cgcaggaagt tcaaaatgcc cagcatcagc
     1681 gcgaagagct ggagcgtgag aacaaccatc tgaaagaaca gcagaacggc tggcaggaac
     1741 gtctgcaggc cctgctgggt cgcatggaag aggtctgatt acgcttcatc tttcgcgtaa
     1801 tttctttact gtgtaggcca acaggtaacg cagaaaaaag gcaccttgcg gtgcctttct
     1861 tatcattcaa tatccagcgg atcttctgaa agaataatcc cggtattgtc ggcataaaga
     1921 tggtcgccgg agaagaaggt gacaccgcca aaattgacgc ggacatcgct ttcgccaatg
     1981 ccttcgccag cggcaccaac cggaattgcc gccatcgcct ggatgccgat atccaactct
     2041 tccaggtcat ctacctgacg caccgcgccg taaatgacca gaccttccca ttcattttgt
     2101 actgccagac gcgccagttc agcatcgacc agtgcgcgac gaacagaacc accgccatcg
     2161 acgacaagaa cacgaccacg gccattctgt tcgagcagat cgtacagcaa cccgttgtcc
     2221 tcgaaacatt ttaccgtgat tatttgtccg ccaaacgacg cccgtccgcc aaagttggag
     2281 aacagcggtt ccacgacgtt aacatcttct tgatagatgt cacaaagctc ggaagtatcg
     2341 tatttcatag gcttaacatt cagttgctgc gagaattttc agtatatcgc gctatgtggg
     2401 ctgttggcaa agtcatcaat tgttaattga tatttgtcag ttatgctgcc cactggctta
     2461 ggaatatccc taaaacaaac agcaggttag tcagtaacgc tcccttgaca gtacgttcca
     2521 gcattggtcg catcgccacc gggtccattt cccgcatcac ataacgggct tgcttcacca
     2581 gtaatggtgc cgccagcagg aacagccagc cccacaggct atgcagcgaa aagagattaa
     2641 acagcgccag acacaccagc gagcccatca gcaggcaggc atgataacga cgcgcgttca
     2701 cttcacctaa gcgcaccacc agcgtgtttt tgccattttc gcggtcgcta ttgatatcac
     2761 gcaggttatt aatattcagt actgccgttg ccagcaggcc gcatgcggtc gccggaagga
     2821 tcagtgccgg aatcaatgta tgagcctgta aataccagct ccccatgaca ctcaaccagc
     2881 caaagaaaac cagtacggaa atatcaccca gaccgatata accataagga cgattgccca
     2941 cggtgtaggt gatagcggca atgatcgaca acccgccaag aatcaggaaa ccgacaaaat
     3001 cggccagcgt atggcatgcc actgcaacca gtgccagccc ggagagacag atgagcacga
     3061 cggtaataat gagcgcccgt ttcatctttt gctgggtaat gacccctttt tgcatgccgc
     3121 gtagcggccc aatgcggtca ggtttatcgc tgccttttac cgcatcgccg taatcattgg
     3181 cgaggttaga aaggatctgt aatagcccgg cggtaattag tgccagcagg gcgaccagcg
     3241 gatcgaagtg accttgccac catgccagcg ccgtcccgac gataattgca gcaaaggcga
     3301 gggggagggt tttaggtcgt aaactttcca gccacgcctg agttcggcta atttgttgtt
     3361 cagtcataat acgcgccaat aaaaatgggg cctttcagcc ccatcaaaca atgatgaaaa
     3421 tgattgaacg cgattatagg ataaaacggc tcagatcttc atctgccacc aacgcatcca
     3481 gatgtttgct cacataatct gcgtcaatag tgatattttg accgcttaaa tcgctggcgt
     3541 cgtaggaaat ctcttccatt aaacgctcca gaacagtgtg taaacgacga gcaccgatgt
     3601 tttcggtaga ttcgttcacc tgccatgccg cttccgcgat gcgtttaata ccggagtcgg
     3661 taaactcgat atttacgcct tcagtcgcca tcagtgcttt gtactgcacg gtgatagagg
     3721 cattcggctc ggtcagaata cgctcgaagt cgctggtggt cagtgcctgc agttcaacgc
     3781 ggattggcag ccgaccttgc agttccggga tcaggtcaga cggtttcgca atctggaacg
     3841 cgccagaagc gataaacaga atgtggtcag ttttgaccat cccgtgtttg gtggaaacgg
     3901 tgcaaccttc taccagcggc agcaggtcac gctgaacgcc ttcacgagaa acatccggac
     3961 cggaagactc gccgcgctta cagattttgt cgatttcgtc gataaacacg atcccgtgct
     4021 gctcaacagc gtcgatagcg tcttgcttca gctcttccgg gttcaccagt ttcgccgctt
     4081 cttcttcaat cagcagcttc atggcgtctt tgattttcag cttacgcgct ttttgcttct
     4141 ggccgcccag gttctggaac atggactgca gctggctggt catctcttcc atgcccggag
     4201 gagccataat ttcaacgccc atcggtgctg cggcaagatc gatctcgatt tctttgtcat
     4261 caagctggcc ttcacgcagt tttttgcgga atgcctgacg agcagcggaa ggttcctgct
     4321 gctgttcagt ctgtccccag ttgtttttag caggtgggat cagcacgtcg agaatacgtt
     4381 cttctgccag ttcttcagcg cgataacggt ttttctcgat agcctggacg cgtaccattt
     4441 tcacggcggc atcggtcaga tcgcgaataa tagaatcaac ttccttaccg acgtagccca
     4501 cttcggtaaa tttggtcgct tcaactttga tgaacggcgc attcgccagc ttagccagac
     4561 gacgggcgat ttcagtttta ccgacaccgg tcgggccgat catcaggata tttttcgggg
     4621 tcacttcatg gcgcagctct tcgttgagct gcatgcgacg ccagcggtta cgcagagcaa
     4681 tcgccacaga acgcttggcg ttgtcctggc cgatgatgtg cttatccagt tcgctgacga
     4741 tttcgcgtgg ggtcatttca gacatgggag atccttacgc tttgtagctt aattcttcga
     4801 tggtgtggaa atggttggta tagatgcaaa tgtcgcctgc aatatccaac gccttttcag
     4861 caatttcacg ggcgctaagt tcagtgtttt ctaacagcgc gcgcgccgca gcctgggcgt
     4921 aagggccgcc ggagccgata gcaataagat cgttttctgg ctgcaccacg tcaccgttac
     4981 cggtgatgat aagcgatgca gtttcatccg cgactgccag cagtgcttca agtttgcgca
     5041 gcatgcgatc ggtacgccag tcttttgcca gctcaacggc ggctttaacc agatggccct
     5101 gatgcatttc cagtttacgt tcaaacagtt cgaacagcgt aaaagcatcc gcagtaccgc
     5161 ccgcaaagcc cgcgatgact ttgtcgttgt acagacggcg gacctttttc acgttgcctt
     5221 tcattacggt attgcccaac gtggcctgac catcaccagc gatgaccaca tggccgttac
     5281 ggcgtacgct tactatagtt gtcacgagct gaccccttgg ttacgaatac agagtacaaa
     5341 ccccgtacaa aagtacgggg cataatgcaa ttatagatgg gggggatttt gagggtttca
     5401 acccccggcg gcgagccgaa tgcagtttgt atgacccgcc atcttcaacc gattgagggt
     5461 gctgtctgcg ttctctttgc ctttcaccgg gccaatgacc acacgattcc agccattgtt
     5521 ggtggtgatt ttcgagtcaa atccttcgaa cgccagctga gcacgtaccg tctctgcctg
     5581 ttccgcgcct ctgaacgaac cgcactgcac catccagcgg cgttcgtctt ttttctccgc
     5641 cgtcggtttt ggcgcgtcag cggcacgagc aaccggcgca gcttgctgcg gcttcgactg
     5701 cgcggtcgtg tgcgcagggg tttgcagcag atcctggtac ggctgctgag tagaagctgg
     5761 tttagactgg cgcggttgcg cctgtaccgg cgcggcttgc gacgtacgcg tctgctgctg
     5821 ccagctttgt tcagtcgtac gcgactgctg agccaggcgt tgctgctccg ctaattgctg
     5881 cgcctgacgc tggcgttgta gcgtttgctg acgctgttcc ggcgtctgtt cattccacgg
     5941 aacctcaacc agctgcgttg gctgctggcg catatcagcc tgcatttgtt caagaagctg
     6001 acgttgttct ggtgtcagtt gctccggcgt tttcacttca ccaccggcag aaggttctgt
     6061 gggcgcacgc acacccggct ggcgactttc cagctcttta atgtagcgcc agcgttcttc
     6121 tggttttggt ggtagtccgt ttccggtcac tttctggctt tgcagcgtct cggactcttc
     6181 tttcttgtga tgcgtaatga agtacagacc accgataaag gtcacaagaa cggcggcagc
     6241 aatagcgacc atagcgggag aaaccgcagg cagatttcgt tgctttttcc gtgaggtgct
     6301 cttttttcgc cgcgaaggtg ccggttggct gcggcgtaca taatctcgtt gtgccactat
     6361 cgtttcgctg tatttattcg ttcgtcagcc cgccatgtta cttaagcggc gggcctttga
     6421 ccagtcacgg cagtcttaaa ggtttacttt aaggtaacgc gcgtgttgat ccccggatga
     6481 taagttcgca gtccattaaa cgagagccac tgccaacgtg ttgcccctgc atttgatcaa
     6541 gcaataacag catagcttcc cgaccgattt cgtaacgcgg ctgcgcgatg gttgtcagcg
     6601 gcggatcaca aaattgcgtc aggtcgatgt tatcaaaacc gattatggaa aggtcttccg
     6661 ggactttcag cccctggcgt tttgcctgag aaagtgcgcc gagcgccatc acatcgctat
     6721 ggcagaagac tgcagtaggc ggttgtggaa gatcaagcag ctgttgcatt gctttgcttc
     6781 cggcttcgaa ggtgaagtcg ccacgggcga tgtattgtgg atcaaccata attccgcagc
     6841 gacgcagcgc ctgaacatag ccttgcaggc ggtagtgaca cagtggcatc tcttcgggac
     6901 cggctataca gccaatccgt ttatgccctt gctcatataa ataatttact gcatcaaatg
     6961 cggcggtcag attgtcgata tgaacggtag gcagctccag ttccggtgca aattcgttcg
     7021 ccatcaccat cggcggcaga ttacgctgtt cctcaatgct ggcatcaaac ggcagccttg
     7081 aacccagcag caacatgcca tcaatttgct tggtgatgat caaatcgata aaggtttttt
     7141 cctgctgatt ttgatgcgca cagtcgccaa tcagcaccag atatccgtga tttgccgccg
     7201 taacttcgat accgcgaata atttcgctaa agaagggatc gcagatatcc gggacaatca
     7261 ccagaatggt gcgggattca ttacgcttga cgttgcgccc cataggctgc ggtaaataac
     7321 ccacttcccg ggccgctttt tcaacccgat tacgggtggc ctgggagact ttatcgggat
     7381 tcattaatgc tcgggagaca gtcgctgtag agacttttgc cttgagggca acgtctttca
     7441 tggtcgcggc agtttcctgc ttcttcgctt tcacactcac tcctcgcctg gcacgtcagg
     7501 cgtactacat ccatgtttac ttcacatcgg caacattttt agcagatagc gcgtgaaaac
     7561 ggttacagaa ttttcatgaa aagtgtgatg aatattgaat ttttcgatcc gcctcgcatc
     7621 gtaagcggtt taaccctcaa tcggatcaac atccagtacc catttcacct tgcgggaatc
     7681 cggtattgta ttgatgagcg ccagcgtacc actaatgatg tgttgcaagc gcacgcggga
     7741 agggtgctgc aacaatatct gccagcgcca gcggccgcca cgtttaggtg ccagtgccgg
     7801 aaccggaccg agaacccaca gtttgtcgtc tgccagcgga ctggagagga tcagattacg
     7861 cagttgttgc aggaacagcg gcgcatgctg attgttatga tcttccgcac gcacaatcac
     7921 atggctggtc catggcggca gttgcatcat ccgtcgctca gccagcgcct gttcggcaaa
     7981 ggcgtcgtag cctttataga gcaacgtttg caacagagga tgttccggat ggtgcgtttg
     8041 cagcaccact tcgccttgtt tacccgcacg cccggcacga ccagcgacct gggtgtaaag
     8101 ctgagcgaaa cgctctgccg agcgaaaatc ggcagaaaac agcgcgccgt ccacatccag
     8161 taacgccacc agcgtcacat ccggaaagtg gtgacctttc gccagcattt gtgtgccgat
     8221 caaaatcctc gcaccgccgc gatgtacttc cgccagttgc tgttccagcg cccctttgcg
     8281 gctggtggta tcgcggtcga tacgagaaat gggcacatca gggaacagcg gcgcaagcgt
     8341 ctgttcaagc tgttcggtgc ccagccccac gggtaccagg tgcgtggaac cgcaggaagg
     8401 gcactggcgc ggcaccggac gctgactgtc acagtggtga cagcgcaaat gctgttgcgc
     8461 ctgatgcagc gtgtagtagt gatcgcaacg tgggcattcg gcaatccagc cgcagtcgtg
     8521 gcacagtagc gcaggcgcaa agccacggcg gttaagaaag aggataacct gattgttggc
     8581 ctgtaaatgc tggcgcatac gggtgattaa cgccggagcc agacctgcct gcaccttctg
     8641 accttttaaa tccagcacgt gttgaattgc cggacgcgca ttccccgccc ggcgcgtcag
     8701 gcgcagcagg cggtattttt tctgctggac gttgcacaac gtttccagcg cgggcgttgc
     8761 ggagccaaga ataatcggga tttgctcgct atgcgcacga tacaccgcca ggtcgcgggc
     8821 atgatagcgc cagccttcct gctgcttgta ggaactgtcg tgctcttcgt caatgacaat
     8881 cacgccgaga tttttaaacg gcgtaaacag cgcggagcgg gtgccgatca caatcgccgc
     8941 ctcgccattt ttcgctttca gccacgccga aagacgctcg ctgtcgttca gaccggaatg
     9001 cagaacttcc acgggcgcat taaaccgttc acgaaaacgg gcgatagttt gcggtgtcag
     9061 gccgatttcc ggcaccatca ccagcgcctg tttgccctga gcgagcacgt tttccagtac
     9121 actgagataa acctccgttt taccggaacc ggtaacgccc gccagcagcc aggcagaaaa
     9181 agtatctgcc gcgctatgaa ttgcgccaac ggcggtggcc tgttcggtat tcaatcgcaa
     9241 ccgctcacca gaaacggcat agttcgttcg ccagtcgcta aactctggtg tttcacttgc
     9301 taaatcacac agaccttttt tgcgcagcgc ctgcaacgcg gcatcattaa attcgagcgt
     9361 ggcaacctgg tcgcgccaga ttttgccttg ccgtaacgct gccagcgcct gttgttgctt
     9421 gggtgagcgt ttcaggctgt tcagatccac cgcctgacct tgttcagtgg caaaccagta
     9481 ccacatcggc gcgttcgccg caggccgccc ctggcgtagt aaaatcggca aggcatgaaa
     9541 cagcacatcg ccaatcggat gatgatagta atctgccgcc catagcagca atcgccagac
     9601 ggagtgagta aacaccggct caccatccag cacttcgact accgctttta gctcagtgag
     9661 cggcagttcg ctggcatcgc taactgatac cacaatcccg atgcgctcct gctgtttgcc
     9721 aaacggcacg cgcacgcgac acccagcttt aaccgtcatg ccttctggca gcagatagtc
     9781 aaaggtacga ggaagcggaa cgggcaaggc aacgtgggca acgggcatag catcatcctg
     9841 acttgaaatt cggtgggtta gtatacacat tgccgtagaa gagtgcggat cagtttgcat
     9901 acgctggtta atttctgtat gatttcgcgc cttcgtacga aatgatcgta ttgaagctat
     9961 acttttaaca tcgcgtggtg tctggcgtta gggctggaag agcgacgcgg ccttaaaccg
    10021 aggttttccc atgaaaaaag atattcaccc gaaatacgaa gaaattactg ctagctgctc
    10081 ttgcggtaac gtaatgaaaa tccgctccac cgttggtcat gacctgaacc tcgacgtgtg
    10141 cagcaagtgc cacccgttct tcactggcaa acagcgtgat gttgctaccg gtggccgtgt
    10201 tgaccgcttc aacaagcgtt tcaacatccc gggcagcaaa taagaccgga tttccgaaaa
    10261 aagcgccgca cggcgctttt tttgtgcctg aattttggtc gtatcaccac gaaaacagcg
    10321 gccactcttt ggcaaccgtg gtgagttgtg gcgcgtcgaa aacggcttgt ggtgaaacta
    10381 ccgtttcctc aagcggaata tttttaccgt agcgttcttt gagcttcgcc tggaccagcg
    10441 ggttgctgag atcaaactct ttcaacttct gctgttcgcc cacgcgcatt cccagcgcgt
    10501 tctggtaaac cttccacacc acttccgaac agtactggcg atcgtcgctc catgagaagc
    10561 tgaaatcata cggtttaccg agataacgtt ttgccgtttg cgtcagtttt tgctgttgtt
    10621 caacactcag tccgccttca acgcggcgaa caacgtagtt gcccttttca ccatgggcaa
    10681 tccactgctt gagcggggtg tatttcaccg ggccgaccgc ttcaaaaacg tagggctttt
    10741 tgttacgcat caccagcata ccggtgtggc tgtaatcgga atgggttgcc agttggatcg
    10801 ctttactttg cgatgagcga gagatttgaa agatgatgtc gccagtttgc ggctgccagg
    10861 caaaggcagg tacagaaacc agcaggctga ggatcagcag cctgtttttc atagttaaac
    10921 gtccatgtat aaaaagcggt gggtcgcaga caacgtgctc gttgtttatg ccggatgcgg
    10981 cgtgaacgcc ttatccggcc tacaagttcg tgcaaattca ataaattgca atatgacgta
    11041 ggcctgataa gcgtagtgca tcaggcgatt ccactccgcg ccgctctttt ccgctttagt
    11101 attcccacgt ctccgggtta atccccatct cacgcatgat ctcttttgcc gcttccggga
    11161 tttcgtcgct gcgctcttta cgcagatcgg catcatccgg caaaggttgc ccggtaaagg
    11221 catgcagaaa cgcttcgcac agcagctcgc tgttggtagc gtgacgcagg ttgttcacct
    11281 gacgacgcgt gcgttcatcg gtgaggattt ttaacacctt aagaggaatg gaaaccgtaa
    11341 tctttttgac ttgttcactc ttcttgccgt gttcagcgta tgggctgata tattcgccgc
    11401 tccattcagc catgagatac ttaatcctct tcgtcaataa attgagacca gaccccacaa
    11461 tttatgtggg tactgaccgt aaacccgcat agtttaccgt acaggcgtta ccgtgacatc
    11521 gtgtaatgca cctgtcggcg tgataatgca tataatttta acggctattt gggatttgct
    11581 caatctatac gcaaagaagt ttagatgtcc agatgtattg acgtccatta acacaatgtt
    11641 tactctggtg cctgacattt caccgacaaa gcccaggaaa cttcatcaca tgacgcgtaa
    11701 acaggccacc atcgcagtgc gtagcgggtt aaatgacgac gaacagtatg gttgcgttgt
    11761 ccctcccatc catctttcca gcacctataa ctttaccgga tttaatgaac cgcgcgcgca
    11821 tgattactcg cgtcgcggca acccaacgcg cgatgtggtt cagcgtgcgc tggcagaact
    11881 ggaaggcggt gctggcgcag tacttactaa taccggcatg tccgcgattc acctggtaac
    11941 gaccgtcttt ttgaaacctg gcgatctgct ggttgcgccg cacgactgct acggcggtag
    12001 ctatcgcctg ttcgacagtc tggcgaaacg cggttgctat cgcgtgttgt ttgttgatca
    12061 aggcgatgaa caggcattac gggcagcgct ggcagaaaaa cccaaactgg tactggtaga
    12121 aagcccaagt aatccattgt tacgcgtcgt ggatattgcg aaaatctgcc atctggcaag
    12181 ggaagtcggg gcggtgagcg tggtggataa caccttctta agcccggcat tacaaaatcc
    12241 gctggcattg ggtgccgatc tggtgttgca ttcatgtacg aaatatctga acggtcactc
    12301 tgacgtggtg gctggcgtgg tgattgctaa agacccggac gttgtcactg aacttgcctg
    12361 gtgggcaaac aatattggcg tgacgggcgg cgcgtttgac agctatctgc tgctacgcgg
    12421 gttgcgaacg ctggtgccgc gtatggagct ggcgcagcgc aacgcgcagg cgattgtgaa
    12481 atatctgcaa acccagccgt tggtgaaaaa actgtatcac ccgtcgctgc cggaaaatca
    12541 ggggcatgaa attgccgcgc gtcagcaaaa aggctttggc gcaatgttga gttttgaact
    12601 ggatggcgat gagcagacgc tgtgtcgttt cctgggcggg ctgtcgttgt ttacgctggc
    12661 ggaatcatta gggggagtgg aaagtttaat ctctcacgcc gcaaccatga cacatgcagg
    12721 catggcacca gaagcgcgtg ctgccgccgg gatctccgag acgctgctgc gtatctccac
    12781 cggtattgaa gatggcgaag atttaattgc cgacctggaa aatggcttcc gggctgcaaa
    12841 caaggggtaa aaatgagtgt gattgcgcag gcaggggcga aaggtcgtca gctgcataaa
    12901 tttggtggca gtagtctggc tgatgtgaag tgttatttgc gtgtcgcggg cattatggcg
    12961 gagttctctc agcctgacga tatgatggtg gtttccgccg ccggtagcac cactaaccag
    13021 ttgattaact ggttgaaact aagccagacc gatcgtctct ctgcgcatca ggttcaacaa
    13081 acgctgcgtc gctatcagtg cgatctgatt agcggtctgc tacccgctga agaagccgat
    13141 agcctcatta gcgcttttgt cagcgatctt gagcgcctgg cggcgttgct cgacagtggt
    13201 attaacgatg cggtgtatgc ggaagtggtg ggccacggtg aagtgtggtc ggcgcgcctg
    13261 atgtctgcgg tacttaatca acaagggctg ccagcggcct ggcttgatgc ccgtgagttt
    13321 ttacgcgctg aacgcgctgc gcaaccgcag gttgatgaag ggctttctta cccgttgctg
    13381 caacagctac tggtacaaca tccgagcaaa cgtctggtgg tgaccgggtt tatcagccgt
    13441 aacaacgccg gtgaaacggt gttgctgggg cgtaacggtt ccgactattc cgcgacacaa
    13501 atcggtgcgc tggcgggtgt ttctcgtgta accatctgga gcgacgtcgc cggggtatac
    13561 agtgccgacc cgcgtaaagt gaaagatgcc tgcctgctgc cgttgctgcg tctggatgag
    13621 gccagcgaac tggcgcgtct ggcggctccc gttcttcacg cccgtacttt acagccagtt
    13681 tctggcagcg aaatcgacct gcagctgcgc tgtagctaca cgccggatca aggttccacg
    13741 cgcattgaac gcgtgctggc ctccggtact ggtgcgcgta ttgtcaccag ccacgatgat
    13801 gtctgtttga ttgagtttca ggtgcccgcc agtcaggatt tcaaactggc gcataaagag
    13861 atcgaccaga tcctgaaacg tgcccaggtg cgtccactgg cggttggcgt gcataacgat
    13921 cgccagttgc tgcaattttg ctacacctca gaagtggccg acagtgcgct gaaaatcctc
    13981 gacgaagcgg gattacctgg agaactgcgc ctgcgtcagg ggctggcgct ggtggcgatg
    14041 gtcggggcgg gtgtcacccg taacccgctg cattgccacc gcttctggca gcaactgaaa
    14101 ggccagccgg tcgagtttac gtggcagtcc gatgacggca tcagtctggt ggcagtactg
    14161 cgcaccggcc cgaccgaaag cctgattcag gggctgcatc agtccgtctt ccgcgcagaa
    14221 aaacgcatcg gcctggtatt gttcggcaaa ggcaatatcg gctctcgctg gctggaactg
    14281 ttcgcccgcg agcagagcac gctttcggcg cgtactggct ttgaatttgt gctggcaggc
    14341 gtggtggaca gccgccgcag tctgttgagt tatgacgggc tggacgccag ccgcgcgtta
    14401 gccttcttca acgatgaagc ggtcgagcag gatgaagagt cgcttttcct gtggatgcgc
    14461 gcccatccgt atgatgattt agtggtgttg gacgttaccg ccagccagca gcttgctgat
    14521 cagtatctcg atttcgccag ccacggtttc cacgtcatca gtgccaacaa actggcggga
    14581 gcaagcgaca gcaataaata tcgccagatc cacgacgcct tcgaaaaaac cgggcgtcac
    14641 tggctataca acgccaccgt tggtgcgggc ttgccgatca accataccgt gcgcgatctg
    14701 atcgacagcg gcgataccat tttgtcgatc agtgggatct tctccggcac gctctcctgg
    14761 ctgttcctgc aattcgacgg tagcgtgccg tttaccgagc tggtggatca ggcgtggcag
    14821 cagggcttaa ccgagcctga tccgcgtgac gacctctccg gcaaagacgt aatgcgcaag
    14881 ctggtgattc tggcgcgtga agcgggttac aacattgaac cggaccaggt acgtgtggaa
    14941 tcgctggtgc ctgctcattg cgaaggtggc agcatcgacc atttctttga aaatggcgat
    15001 gaacttaacg agcagatggt gcaacggctg gaagcggccc gcgaaatggg gctggtgctg
    15061 cgctacgtgg cgcgtttcga tgccaacggc aaagcgcgtg tgggcgtgga ggcggtgcgt
    15121 gaagaccatc cgctggcatc actgctgcca tgcgataacg tctttgccat cgaaagccgc
    15181 tggtaccgcg ataatccgct ggtgatccgt gggcctggcg ctgggcgcga cgtcaccgcc
    15241 ggggcgattc agtcggatat caaccggctg gcacagttgt tgtaatttca gaaatttaat
    15301 aatgcccggt actcatgttt tcgggtctat ggtttctaat gaaatatatt gaattatcat
    15361 agaattaggc cggatgaagc gtttacaacg aatccggcaa gaagcaataa gtacatggtt
    15421 agtttatatt tgcagtccgg tttgctttgc atatcggatt ttctttttct taccatcctg
    15481 aagttttttc atcttccctg atttttcctc accatcattg gtcatttttc ggttgacgcc
    15541 cttcggcttt tccttcatct ttacatctgg acgtctaaat ggatagatgt gcacaacaca
    15601 acatataact acaagcgatt gatgaggtaa ggtatgagct tttttcacgc cagccagcgg
    15661 gatgccctga atcagagcct ggcagaagtc caggggcaga ttaacgtttc gttcgagttt
    15721 ttcccgccgc gtaccagtga aatggagcag accctgtgga actccatcga tcgccttagc
    15781 agcctgaaac cgaagtttgt atcggtgacc tatggcgcga actccggcga gcgcgaccgt
    15841 acgcacagca ttattaaagg cattaaagat cgcactggtc tggaagcggc accgcatctt
    15901 acttgcattg atgcgacgcc cgacgagctg cgcaccattg cacgcgacta ctggaataac
    15961 ggtatttgtc atatcgtggc actgcgtggc gatctgccgc cgggaagtgg taagccagaa
    16021 atgtatgctt ctgacctggt gacgctgtta aaagaagtgg cagatttcga tatatccgtg
    16081 gcggcgtatc cggaagttca cccggaagca aaaagcgctc aggcggattt gcttaatctg
    16141 aaacgcaaag tggatgccgg agccaaccgc gcgattactc agttcttctt cgatgtcgaa
    16201 agctacctgc gttttcgtga ccgctgtgta tcggcgggca ttgatgtgga aatcatcccg
    16261 ggcattttgc cggtatctaa ctttaaacag gcgaagaaat ttgccgacat gaccaacgtg
    16321 cgtattccgg cgtggatggc gcaaatgttt gacgggctgg atgacgatgc cgaaacccgc
    16381 aaactggtgg gcgcgaatat tgccatggat atggtgaaga ttttaagccg tgaaggagtg
    16441 aaagatttcc acttctatac gcttaaccgc gctgaaatga gttacgcgat ttgccatacg
    16501 ctgggtgttc gacctggttt ataaatagtg tggcttttgt gaaaatcaca cagtgatcac
    16561 aaattttaaa cagagcacaa aatgctgcct cgaaatgagg gcgggaaaat aaggttgtca
    16621 gccttgtttt ctccctcatt acttgaagga tatgaagcta aaaccctttt ttataaagca
    16681 tttgtccgaa ttcggacata atcaaaaaag cttaattacg atcaatttga tctacatctc
    16741 tttaaccaac aatatgtaag atctcaacta tcgcatccgt ggattaattc aattataact
    16801 tctctctaac gctgtgtatc gtaacggtaa cactgtagag gggagcacat tgatgagcac
    16861 gtcagacgat atccataaca ccacagccac tggcaaatgc ccgttccatc agggcggtca
    16921 cgaccagagc gcgggggcgg gcacaaccac tcgcgactgg tggccaaatc aactccgtgt
    16981 tgacctgtta aaccaacatt ctaatcgttc taacccgctg ggtgaggact ttgactaccg
    17041 caaagaattc agcaaattag attactacgg cctgaaaaaa gatctgaaag ccctgctgac
    17101 agaatctcag ccgtggtggc cagccgactg gggcagctac gccggtctgt ttattcgtat
    17161 ggcttggcac ggcgcaggta cttaccgttc aatcgatgga cgcggcggcg cgggtcgtgg
    17221 tcagcaacgt tttgcaccgc tgaactcctg gccggataac gtaagcctcg ataaagcgcg
    17281 tcgcctgttg tggccaatca aacagaaata tggtcagaaa atctcctggg ccgacctgtt
    17341 tatcctcgca ggtaacgtgg cgctggaaaa ctccggcttc cgtaccttcg gttttggtgc
    17401 cggtcgtgaa gacgtctggg aaccggatct ggatgttaac tggggtgatg aaaaagcctg
    17461 gctgactcac cgtcatccgg aagcgctggc gaaagcaccg ctgggtgcaa ccgagatggg
    17521 cctgatttac gttaacccgg aaggcccgga tcacagcggc gaaccgcttt ctgcggcagc
    17581 agctatccgc gcgaccttcg gcaacatggg catgaacgac gaagaaaccg tggcgctgat
    17641 tgcgggtggt catacgctgg gtaaaaccca cggtgccggt ccgacatcaa acgtaggtcc
    17701 tgatccagaa gctgcaccga ttgaagaaca aggtttaggc tgggcgagca cttacggcag
    17761 cggcgttggc gcagatgcca ttacctctgg tctggaagta gtctggaccc agacgccgac
    17821 ccagtggagc aactatttct tcgagaatct gttcaagtat gagtgggtac agacccgcag
    17881 tccggctgga gcaatccagt tcgaagcggt agacgcaccg gaaattatcc cggatccgtt
    17941 cgatccgtcg aagaaacgta aaccgacaat gctggtgacc gacctgacgc tgcgttttga
    18001 tcctgagttc gagaagatct ctcgtcgttt cctcaacgat ccgcaggcgt tcaacgaagc
    18061 ctttgcccgt gcctggttca aactgacgca cagggatatg gggccgaaat ctcgctacat
    18121 cgggccggaa gtgccgaaag aagatctgat ctggcaagat ccgctgccgc agccgatcta
    18181 caacccgacc gagcaggaca ttatcgatct gaaattcgcg attgcggatt ctggtctgtc
    18241 tgttagtgag ctggtatcgg tggcctgggc atctgcttct accttccgtg gtggcgacaa
    18301 acgcggtggt gtcaacggtg cgcgtctggc attaatgccg cagcgcgact gggatgtgaa
    18361 cgccgcagcc gttcgtgctc tgcttgttct ggagaaaatc cagaaagagt ctggtaaagc
    18421 ctcgctggcg gatatcatcg tgctggctgg tgtggttggt gttgagaaag ccgcaagcgc
    18481 cgcaggtttg agcattcatg taccgtttgc gccgggtcgc gttgatgcgc gtcaggatca
    18541 gactgacatt gagatgtttg agctgctgga gccaattgct gacggtttcc gtaactatcg
    18601 cgctcgtctg gacgtttcca ccaccgagtc actgctgatc gacaaagcac agcaactgac
    18661 gctgaccgcg ccggaaatga ctgcgctggt gggcgggatg cgtgtactgg gtgccaactt
    18721 cgatggcagc aaaaacggcg tcttcactga ccgcgttggc gtattgagca atgacttctt
    18781 cgtgaacttg ctggatatgc gttacgagtg gaaagcgact gacgaatcga aagagctgtt
    18841 cgaaggacgt gaccgcgaaa ccggcgaagt gaaatacaca gccagccgtg cggatctggt
    18901 atttggttct aactctgtcc tgcgtgcagt agcggaagtt tacgccagca gcgatgccca
    18961 cgagaagttt gttaaagact tcgtagcggc atgggtgaaa gtgatgaacc tcgaccgttt
    19021 cgacctgctg taatctgacc ttcttcagcg actgtctttc aggcagtcgc tgaagtttct
    19081 ttaccggcgt atagtgtcca caggaaaact acacactgga tctctcatgt ctgccgcagg
    19141 aaagagcaac ccactggcaa tcagtggcct ggttgtgctc acacttatct ggagttatag
    19201 ctggattttc atgaagcaag tcaccagtta catcggtgcc ttcgacttta ccgccttacg
    19261 ctgcattttc ggcgctctcg ttttattcat cgtcctttta ttacgtggtc gcggaatgcg
    19321 cccgacgccg tttaaataca ccttagccat tgccctgtta caaacctgcg ggatggttgg
    19381 tctggcgcag tgggcgttgg tcagcggagg tgcggggaag gtggcgatcc tgagctatac
    19441 catgccgttc tgggtggtga ttttcgccgc gttgtttctc ggtgaacgcc tgcgacgtgg
    19501 gcaatatttc gcgattctga ttgccgcttt cggcttattt ttggtgttgc agccgtggca
    19561 actcgatttc tcttcgatga aaagtgccat gctggcaatc ctctccggtg ttagttgggg
    19621 ggcgagcgcg attgttgcta aacgcctata cgcccgtcat ccgcgcgtgg atttattatc
    19681 gttaacatcc tggcagatgc tgtatgcggc gctggtgatg agtgtggtcg ctttactggt
    19741 gccgcaacgt gaaattgact ggcagcccac cgtgttctgg gcgctggcct acagtgcgat
    19801 tctggcgacg gcgctagcgt ggagcttatg gctgtttgtg ctgaaaaact tgcctgccag
    19861 tattgccagc ttaagcacac tggccgttcc cgtttgtggc gtactctttt cctggtggct
    19921 gctcggtgag aatccggggg ccgttgaagg tagcggtatt gtgctgattg tgctggcact
    19981 ggcgctggtg agccgtaaga aaaaagaagc tgtcagtgta aaaagcatct gaattttttc
    20041 ttcatgtggg gcgatctctt atttaacaaa ataacgataa tgccccacca tccgccagct
    20101 aaacagcaca tcttcttcct gcgcgcctgc gccaatgtta tgtatcacca gcggcgtacc
    20161 gtcgcgggcg aagccatctg acgccacccc aatatgcgcc agcccgttat ctaatcgcca
    20221 ggagacaata tcgcccgctt gatagtcacc ggcattttta ctgatggggc gtgttttgtt
    20281 gtggcggcta aaccaggttt ccagattagg cacccgacgg tgatcgatgt tgctgtccgg
    20341 gcgctttaac ttccattttt gcgggtactc agcaaaattc ttcgccatat cttcgtgaac
    20401 cagtttctgc aaatcgacct tctggctgcg caatgcgcgg atcaccacat cggaacatac
    20461 accgcgttct tgcggaacat caccgccagg ataggtaagc tgcatatacg ccggatcgta
    20521 aaatagcgtg ctgccaattt gctgtctggc accgtctgcg atggcaaggt tggtattggc
    20581 ctggatttgt accacggttg gtggaacggc gggagatttt aaggagtggc tggtaaatgc
    20641 cgttagcagg ctgagcagcg ccagtgaagc tttcatctgg ttcgtccgtg aaaataagag
    20701 tcacagattc gaccttccgg gctaacgatt gttctgtgga gaaaatgtcc agaagcggat
    20761 aaaaaactcc tgaacaacgt gggagtttca gattactggc acagagtgcc ggatgcggag
    20821 tgaacgcctt atccggccta caaaagcacg caaattcaat atattgcaga gattgcgtag
    20881 gcctgataag cgtagcgcat caggcaattt tgcgttcaaa ctcccggaca agccgggagt
    20941 ttggagtagg ttattcccac tcttgcagga aacgctgacc gtactggtca gctaccagca
    21001 gtgcggcgta aacctgatct ggcgtcgcgc cgccaggcat gttgtggatg gtttcacctt
    21061 ctgcacaaga tgcttttgca acaatgcgca ttttcgccgg gacatcttct ttaatatcca
    21121 gttgagcgag agttattggc aaacctaccg catggctaag tgcagctacg gtttcgattt
    21181 cctccaccgg cgcgttttcc agtaccagct gcgtcagcgt accaaatgcc actttttcac
    21241 cgtgataata gtgatgcgcg tccgggatag cggtcaggcc gttatgcacc gcgtgagccg
    21301 ccgctttcaa aaccaacgcc gctcaaatag gtgttcgctt caatcacgcg ctccagcgcc
    21361 ggggttacta catgctgttc ggcggcaagc atcgcttttt cgccttcttc cagcagggtg
    21421 ttgtagcaca gttcagccag tgccagcgcc gcctgggtgc acttgccgcc cgccatggtg
    21481 gtcgcgccgc tacgagagca agcacgcgct tcaaaccagg ttgccagcgc atcgccgata
    21541 cccgctgcta acagacgtgc aggtgcgccc gcgacgattt tggtgtcgac aatgaccata
    21601 ttcgggttat ttggcaacag cagatagcgg tcaaactcac cctcatcggt gtagataaca
    21661 gacaatgcgc tgcacggtgc atcggtagag gcgatagtcg gtgcgatcgc taccggaaca
    21721 cccatgaaat gtgccagtgc tttggcagta tcgagggttt ttccgccacc gataccgaga
    21781 attgcgccac actgcgcagt ctccgcgatg ccacgcagac ggtcgatctc attttgcgaa
    21841 cattcaccgc caaacggcgt aatttctact accagtccag catctttaaa gcttttctcg
    21901 acagtggatt gagcaaaacc taaaacaaat ttgtcaccca ccactaacca gcgttctgcc
    21961 agcggcttca ggtattcgcc cagacgatta atcacatcag cgccctggat gtatttaccc
    22021 ggtgattgaa taatgcggtc cataattgct cctttagaga tgagtagtgc caaatgcggc
    22081 attccagtcg tgttcgaact tctctatagc tgactctact gcaggggtgt tgagcatttg
    22141 ttgcgctaca tctaagggca gggtgatgga ttcacatcct gccagtaaac agtccagcgc
    22201 ctgacacggc gttttaaagc tggctgccag caccatgctt tctggcgcgt gcatttctaa
    22261 cagcgtttgc agctcctgaa ccgtacgaat gccgtccccg ccctgggcat ctacgcggtt
    22321 aacatacgga gcaacgtatt ttgcccctgc cagtgcggct aataaccctt ctgcggcgct
    22381 atatacagcg gtgccaagtg tagtaatacc ctcttttttc agtattttaa ttgctgccag
    22441 accttcggaa gtcaccggga ttttcaccac aatacccgga atagcgtcgc gcaggcgctt
    22501 cgcttcttcc accatcccct gcgcgtcgcg gctcatggtc tgagcaaaca gaatgccctc
    22561 atcaccaatc gctttttgca gacgcggcag cacttcccag atggactcct tgctggcagc
    22621 gataatgctc gggttagttg tcaccccggc aatcgggaat atgcgtgcca gacgttcgac
    22681 ttctgcgacg ttagcggtgt ccagatacag ttccatgatg tttcctttta cagttccagt
    22741 tcatgttgca gcaggctggc gatagcgtcg gcagaggcgg cgttaaccag cgcgttacgg
    22801 aattcttcgt gcatgatgcg gcgagcgagg cgcgagaaaa tgcgcatgtg ctgatcgccc
    22861 gcagcgtgtt tgttcagggt taacataatg atgaattgcg cttcatcatc gccccagcgc
    22921 accggcgctt gcagacgcgc cacgctgatg gtggattgct caatgtgttc tgatttgctg
    22981 tgtggaatgg caaaactaaa gcccagaccg gtagagaaaa cggcctcgcg cgcccataag
    23041 tcggcttcca gtttacgcgg atagcggcag cggcccgcca gcagcaggtt atcggtcatg
    23101 cctttgagca cttcttcttt gctgcgccag tcactttcca gcgtgatgca ctcggcggtg
    23161 accagcggtg cgtcctgttg ggtcatgcgg aattgcgcca gcaggtgttc cacttccagc
    23221 gaagtacggc aggccattgc ctggttgagc aacttgcggc actcacggct atcaagttgc
    23281 gccatccgcg ctttcgccgc cggaattgat ggtgcgctca tgctgagttc atccagccct
    23341 aagccgacca gcaacggcag cacggaacct ttcgctccca gctcaccgca cagaccaatc
    23401 catttaccct ggcgatgcac cgcctgcacg gcgtaatcga gcgcccgcaa gaatgccgga
    23461 ttcaggctgt tgtagtgacg agtaacctta gcgttatcgc gatccactgc cagcagatat
    23521 tgcgtcaggt cattactacc aatactaaag aaatcaatct cttcgcagca ttgatcgatg
    23581 atgaacatca ccgacggcac ttccagcata atgccgagct ggatcttctc atcaaacgga
    23641 atgtgttcgt tacgtagttg ctgtttggct tctgccagtt tttctttcac ccataagatc
    23701 tcttccattg aggagatcat cgggatcatg attttcaggc tgccgtgagc ggaggcgcgc
    23761 aggatcgagc gtagttgtgt ggtaaataac gacgcgtatt cttcataaat acgcacggcg
    23821 cgataaccga ggaacgggtt tgcctctgcg ggaatgttta gataatcaac gggtttgtcg
    23881 ccgccaatgt ccatagtgcg cacaataatg ctgcgtccgt tggcagactc cagcgcctga
    23941 caaaaaatgt tgtacagctc gctttcgccc ggtgcactgg tgcgatccat atagagcatt
    24001 tcagtgcgga acaaaccaac gccttccgca ccattgccga atgcggcctg cgcttccacg
    24061 gagtgagcga tgttagcggc aatttcaatg cggataccgt cagcggtacg ggcttgttgg
    24121 gtcagccaga cacgctgttg ctcacgcagg gcgtcctgta cgcgggcttc ttgctgataa
    24181 taacgagcta cggcttctcc tggctcaacc acaatcgccc cggcgttgcc gtcgatataa
    24241 atcgtttgat gctgccacgg agtgagggca tcaatatcca caccaaccag cgtcggaatg
    24301 ttgaacgaac gggcaaggat caccgtatgt gaggtggtgc cgccgctttt cagtaacaat
    24361 cctttgaggt gatttttatc cagttcgagg aactggctgg gggtcagttc atcagccata
    24421 caaatggcgg actgcgtcag tttgcccggt gccgggaagc gttgctcacc gtagatttgc
    24481 tggagtaact ggaagcagac gtcgcgtacg tccagggcac gttcttgcag gtatctgctg
    24541 ctggaacggg cgaactcttc gcaaaagtga ttcgcactgg cgacaatcgc ttcggcgcag
    24601 cttaatccgg cgctgactcc tgccagtaaa tgttcgtgca gggaagtatc gccagccagg
    24661 gatcggtgcg cttccagaat cgcgctggta gcaccgtcgc tatccagcag acgaaactca
    24721 atgtttttca gtaccagcgt caggccgttt tccagtgcgg attgctcggc gtcaacgtct
    24781 ttggctgcgg gaagattact cagcgcattg agatctaaag aagagatcgg cgtcagaatg
    24841 ccgcctgcac taccgctgca cacggtgcgg acgcggataa tttgcggatt cagattggtc
    24901 agtgaaaccg gcagtggttc cagttcgtca gatttaactt ccgccagcgg cgcatcgcag
    24961 tgggggaatt catcgcgcag ccattggctt aaacgctggt gcccttcctg ttcgtcggcc
    25021 ccggaaatca gtagctggca gttatcaccc accagcgtat cggtgccaat cagcgccaga
    25081 gcgcttttgg cgttgccttt acggtcagta cgcaggttat gccactcaat ttgagatgaa
    25141 aaagtattac acagcgtttc aacgtggctt gccggacgcg catgtacgcc gttaggtagc
    25201 tcacaaataa attccacaat cagggccatt gcctttctcc cggtacggtt ttctgtttac
    25261 aggataagag cgcacggtaa cggcctgcca tgtgacaaat ctgccaaaag ctggacaaat
    25321 gtaatgtaac cgtcaatttg cgacgcgtct cacaagacgc cgttttgcgg catgcttccg
    25381 gattatcgca agttatgagg cggatcgcat ttttgtactg atattacaaa agtccagtaa
    25441 atggcctttt tatccactgt ttgctccgcc tgcgattgcc tattgtttcc tccatcaaaa
    25501 atatggacat gggcttttgc aagcccggga gcagcttatg aatgagttgg tgcagatcct
    25561 gaaaaatacc cgtcagcatt taatgacggg cgtttcacac atgattccct tcgtggtatc
    25621 gggcggtatt ttgctggcgg tttccgtcat gttgtatggc aaaggcgcag tgccggatgc
    25681 cgtagccgat ccgaatttga aaaaactgtt tgatattggc gttgcaggtt tgacgctgat
    25741 ggtgcctttc ctcgccgctt acattggtta ctccattgca gagcgttctg cgctggctcc
    25801 gtgcgctatc ggtgcctggg ttggtaacag ctttggtgcg ggcttctttg gtgcgctgat
    25861 cgccgggatt atcggcggca tcgtggtgca ttacctgaag aaaattccgg tgcataaagt
    25921 tctgcgctcg gtgatgccca tcttcattat tcctatcatc ggcacactga ttaccgcagg
    25981 catcatgatg tggggcttgg gcgagcctgt aggggcgttg accaacagcc tgactcagtg
    26041 gcttcagggg atgcagcagg gcagcattgt tatgctggcg gtgatcatgg ggctgatgct
    26101 ggcgttcgat atgggcggtc cggttaacaa agtggcctat gccttcatgc tgatttgcgt
    26161 tgctcagggt gtttataccg tggtggccat tgctgccgtg ggtatttgta tcccgccgct
    26221 ggggatgggg ctggcgacgc tgattggtcg taaaaatttc tccgcagaag agcgcgaaac
    26281 cggcaaagcg gcactggtga tgggctgtgt tggcgttact gaaggggcga ttcctttcgc
    26341 cgctgccgat ccgctgcgtg tcattccttc cattatggtc ggttcagttt gtggtgcagt
    26401 aactgcggcg ctggtcggtg cgcagtgcta tgcaggctgg ggtggtctga ttgtgctgcc
    26461 ggtggttgaa ggtaagttgg gttatatcgc agcagtggct gtcggagcag tggtgacggc
    26521 tgtttgtgtg aacgtgctga aaagtctggc gcgtaaaaat gggtcttcga ctgatgaaaa
    26581 agaagacgac ctggatttgg attttgaaat taattaattg aggaacgagc catgacgaaa
    26641 attattgcag taaccgcatg tccttcaggt gttgcccata cctatatggt agcagaggcg
    26701 ctggaaagtg ccgcgaaagc gaaaggctgg gaagtgaaag tagaaaccca ggggtcaatt
    26761 ggtctggaaa atgaactgac tgcggaagac gtggcgagcg ctgatatggt gattctgacc
    26821 aaagatatcg gcatcaagtt tgaagagcgt tttgctggca agaccatcgt gcgcgtcaac
    26881 atcagcgatg cagtaaaacg tgctgacgcc attatgagca aaattgaagc tcacctggcg
    26941 caaaccgctt aagttcttct cccgctcgca agggcgggtt cgctttctca caggagttcc
    27001 tcatgacgaa tcgtatctct cgcctcaaaa ctgcactgtt tgccaatacc cgtgaaatct
    27061 cgctggagcg ggcgctgctt tataccgcca gccatcggca aaccgaaggc gaaccggtga
    27121 tattgcgccg ggcgaaagca acagcgtata tccttgaaca tgttgaaatt tcgattcgtg
    27181 atgaagaact gattgccggt aaccgcaccg taaaaccgcg cgccgggatt atgtcgccgg
    27241 aaatggaccc ttactggctg ctgaaagagc tggatcaatt cccgacgcgt ccgcaggacc
    27301 gctttgctat cagcgaagaa gataaacgta tctaccgtga agagttgttc ccgtactggg
    27361 aaaaacgttc gatgaaagat ttcatcaacg ggcagatgac agatgaagta aaagccgcga
    27421 ccaacacgca gattttcagc atcaaccaga cggataaagg ccaggggcac attattattg
    27481 attacccacg cctgttgaat cacggtctgg gtgagctggt ggcacagatg cagcaacatt
    27541 gtcagcaaca gccggagaat cacttttatc aggccgcgtt gttactgctg gaagcctcgc
    27601 agaaacacat tttgcgttac gccgaactgg cggaaacgat ggcggcaaac tgcacagatg
    27661 cccagcgtcg cgaagagctg ctgactattg cggagatctc ccgccataac gcgcaacata
    27721 agccgcagac gttctggcag gcgtgccagt tattctggta catgaacatc attctgcaat
    27781 acgaatccaa cgccagttcg ctgtctttag ggcgcttcga ccagtatatg ttgccgttct
    27841 atcaggcatc tttaacccag ggcgaagatc cggcgttcct gaaagaactg ctcgaatctt
    27901 tatgggtgaa atgcaacgac atcgtgctgt tgcgctccac cagtagcgcg cgttatttcg
    27961 ccggtttccc gaccggctat accgcactgc tcggcgggtt aaccgagaac ggacgtagcg
    28021 cggtgaacgt gctttcgttc ctttgccttg acgcctatca aagcgtgcaa ttaccgcaac
    28081 cgaacctcgg cgtgcgcact aacgccttga tcgacacgcc gttcctgatg aaaaccgccg
    28141 aaaccattcg cctcggcacc ggtattccgc aaatctttaa cgatgaagtg gtggtgccag
    28201 cgttcctcaa ccgtggcgtt tcgctggaag atgcgcgcga ctattccgta gtgggctgtg
    28261 tggaattatc tattcccggc agaacctacg gcttgcatga catcgcgatg tttaatctgc
    28321 tgaaagtgat ggaaatctgc ctgcatgaaa atgaaggcaa tgctgcgctg acttatgaag
    28381 gtttactgga gcagatccgc gccaagatca gccactacat caccctgatg gttgagggca
    28441 gcaatatttg tgatatcggc catcgcgact gggcacctgt accgctgctc tcgtctttta
    28501 tcagcgattg tctggaaaaa ggtcgcgata ttaccgatgg cggcgcgcgt tataacttct
    28561 ccggcgtaca gggaattggt atcgccaacc tgagcgattc tctccatgcg ttgaaaggga
    28621 tggtttttga gcaacagcgt ttaagttttg acgaattgct gtcggtatta aaagccaact
    28681 ttgcaacgcc agaaggcgaa aaagtccgcg ctcgcttaat taaccgcttt gagaaatacg
    28741 gtaacgatat cgacgaggtg gataacatta gcgccgaact gttgcgccac tactgcaaag
    28801 aagtggaaaa ataccagaac ccgcgcggcg gttacttcac gccgggatcg tataccgttt
    28861 ctgctcacgt tccgttggga tcggtggttg gcgcgacgcc agacggtcgt tttgccgggg
    28921 aacaactggc agacggcggc ttgtcaccta tgctgggtca ggacgcacaa gggccaacgg
    28981 cggtactgaa gtcagtcagt aagctcgata atacgctgct gtctaacggt acgttgctga
    29041 acgtgaaatt cactccggcg accctggaag gtgaagcggg attacgcaaa ctggccgact
    29101 tcttacgggt gtttacccag cttaagttgc agcatattca gtttaacgtg gtgaacgccg
    29161 acacgttgcg ggaagcgcaa cagcgcccac aagattatgc cgggctggtg gtgcgcgttg
    29221 ctggatacag cgccttcttt gtcgaactgt cgaaggagat ccaggatgac atcatccgcc
    29281 ggacagcgca tcagctgtaa cgttgtggaa acgcgccgcg atgatgtggc gcgcattttc
    29341 aacattcagc gttattcact gaatgacggc gagggcattc gtacggtggt cttttttaaa
    29401 ggctgtccgc atctttgccc gtggtgtgct aatccggagt cgatctccgg caaaatccag
    29461 acggtacgca gagaggcgaa atgtctgcac tgtgcgaaat gtttgcgtga tgcggatgaa
    29521 tgcccctccg gggcgtttga acggattggt cgcgatatca gccttgacgc tctggaacgg
    29581 gaagtgatga aagatgacat tttctttcgc acgtccggcg gcggcgtcac gctttctggc
    29641 ggcgaagtgt taatgcaggc ggagtttgct acccgttttt tacagcgact gcggctgtgg
    29701 ggtgtctcat gcgccattga aactgccgga gacgcgccag caagcaagct gttaccgctg
    29761 gcgaaattgt gcgatgaagt gctgttcgat ttaaaaatta tggacgcgac tcaggcgcgg
    29821 gatgtggtga agatgaacct gccacgcgtg ctggagaatc tgcgtttgct ggtgagtgag
    29881 ggtgtcaacg tgatcccgcg tttaccgttg atccctggtt tcacgctcag ccgggagaat
    29941 atgcagcagg cgctggatgt actgatcccg ctgaaaatca ggcagatcca tctgttaccg
    30001 tttcatcagt acggcgaacc gaaataccgc ctgctgggga aaacatggtc gatgaaagag
    30061 gtgcctgcgc cgtcgtcagc cgatgtgaca acgatgcgcg aaatggcaga acgggccgga
    30121 tttcaggtta ccgtgggagg ttaaaatggc atacctggtg gcagtaaccg cctgcgtaag
    30181 cggcgtggcg catacttata tggcggcgga acggctggaa aagttgtgcc agttagagaa
    30241 gtggggcgtc agcattgaaa ctcagggcgc gctgggaacg gagaatcgtt tagcggacga
    30301 ggatatccgt cgggcggatg ttgctctgtt gattacggat atcgagcttg ccggtgccga
    30361 gcgatttgaa cattgccgct atgtgcaatg cagcatctac gcattcctgc gtgagccgca
    30421 gcgggtaatg agcgcggtgc gcaaagtgct ttctgcgccg cagcaaaccc atcttattct
    30481 ggagtagtcg gtttttctgt cagttggctg tggtactgcc ggcgatattc cgacggcgag
    30541 tgttcggtgt ttttacgaaa cagacggcag aagtagttgc tgtcgacaaa gccgcaggcg
    30601 tgcgccactt cttttacctt caggtcatag cctttcaata acgtcttagc gtgctccagt
    30661 cgcgtgtgat tcaggtattc gttaaaacca atggcccccg ttttttgaaa caggtgcgag
    30721 agatagtttg gtgaaatata gaatgcctgt gcgacagatt cgcgggtaag cgcggaggca
    30781 tagcgttcgt cgatataatc gcgaatagct tcaaatagtg cctggctgcg tgaggcggtc
    30841 tggatttggc tgccgcgcaa atcgcggcag tggctaagca ggctggctac gataagcctt
    30901 gccgtttgct gctcctgcgg ctgcatttgc atttcattga gcgtttgtag cagaaaagaa
    30961 cctatgcgtg ggccgcgtcg ggcgacgtgt tgcttcgcca gattttgata ttgtttgcca
    31021 tcccattgca cgacgctgaa gccgagttgc tgtttgccaa acagcacgct aaaggtggta
    31081 gcgggtgttt tccattgtgg gaaattccag ccaccagcgg caacgtacag cacatcgccg
    31141 ggaactaacg ttgctccagc gccggtatca acaaactcac cttccagcac aatttccagc
    31201 cgtggaaaat cgacctgata cgcaagatcg ggcaccgggc cgttactgct ggcaaagtaa
    31261 atctggcgca gggacagcgg gccgttgatc aggcgggaga gcaaatagct gacgtcgtga
    31321 tacatgttat tcccttaggt atgtagacgg gaaaacagta actggcatta ttaaaaaagg
    31381 ccacctgaaa agtgtggcct gaggggagtt cgatgtaaac attgcggcag tatcgcatcc
    31441 ggcaaatagc gccagacgct atacaaaggt catctactga ttacccacct gatcgccata
    31501 cggcagagtg tcgtaatcga tcagcgcgtt tttcttgtac gggttaccaa tccagcgggt
    31561 agtttctttg aactgcggat tcaccactga acgggttgga tcgtaaccgt cgtaagataa
    31621 gcccgccaaa tctgaccagg tgtggatcag ttccgccagg ctgtatttac gatcgacatc
    31681 ctgtgagaaa tcacggggat gagtcgcttg ccatttttcc gacgtccaca gcaggaacgg
    31741 aatggtgtac atgtggcgcg tcgggttgtc ttcgttacgc ccctgagttt tgtgcggcgg
    31801 cgtgtcgtaa acctcttcac cgtggtcaga gaagtaaacc aggaatccgt tcgggtctgc
    31861 cgctttaaag tctttaatca ggctggcaac cacatgatcg ttatacaggt tagcgttgtc
    31921 ataatcgtta tatgattcca gctcttctgc gcttaatcct ggaggaacat gatcggtatt
    31981 gccatcaaac ttgccctgat tttccgggta gcggtatttg tatttgatat gcgtacccag
    32041 cagatgaacg atgatcagtt tcttcggcgc agggtcattc agcacatcct ggaacggctt
    32101 cagcacgttg gtgtcgtatt cacgcgcact ctgcgtacgt tgctggttca tgtagtactg
    32161 cttgtcggtc tggcgcgaaa ataccgtcag catggtattg cgggcggtca tcgtctgctg
    32221 gttggtgatc cagaaggttt tataacccgc ctgtttcatc atgttcatca gcgacggctg
    32281 cgtcagatac agatccgggt tcttttcatt ggcaaaggtc agcgcctgtt gcaggatttc
    32341 aatggtgtac ggacgagacg taactacgtt attaaacacg gtcagattcg gatcggtttt
    32401 atgcagcgca tccagctccg gcgtggtttc acgcggataa ccgtacagac tcatgcgtcc
    32461 gcgctgggtc gactcgccaa tcaccagcac taaagtgcgc ggttcgttac ccgattcatc
    32521 tttgaaatta gccagtggcg gcaaggcatt attttcattc agtaactttg ttagcgagtt
    32581 tagttgctga cgatactgat aatagccggt caggaattgc cacggtgcgg caggctccat
    32641 acgcgaggct aggttatcca acgttttctc aaacggcttg tttttgataa acgtattcat
    32701 ggcgatcgga tgcagaatca agccataaag cagggcaaaa gagacaacat aacgccacgg
    32761 ctttggaata tagaccgggc gcaggcgtgt ccacagcaga actgccaccg ccgtataggc
    32821 cagcgcgata agcacaattt tcaggctgaa atactggctt aaatactcgc tggcttcgtt
    32881 ggtgttggtt tcgaacatca caaacagaac gctctgcgag aactcctgcc cgtagatgac
    32941 gtagtagcac agcgccgcca gagaggccgt ccatagcacc acgccgatca ctgcggcaat
    33001 aattttaatc cgcttcggaa agaggaatac cgggatcaac cacagcgagc tgaataacag
    33061 cgagtcgcga atgccgttgg tgccactata accactgatg taaataatgg cctgtagcag
    33121 agtagagaaa aaccaaaagt agagcagtgc ccaacccagg gctttccagc tagaaagggg
    33181 tttagcctgg acttctgtgg aatgcatagt aagaacctgt cttgaaaaaa atatcgccga
    33241 atgtaacgac aattccttaa ggatatctga aggtatattc agaatttgaa taaaatgcag
    33301 acagaaatat attgaaaacg agggtgttag aacagaagta tttcagaaaa ccctcgcgca
    33361 aaagcacgag ggtttgcaga agaggaagat tagccggtat tacgcatacc tgccgcaatc
    33421 ccggcaatag tgaccattaa cgcttgttcg acgcgaggat ccggttcctg gccttctttt
    33481 tctgcctggc gggagcggtg cagcaactcg gcctgcaata cgttcagcgg gtcggtgtaa
    33541 atattccgta gctgaataga ctctgcaatc cacggcagat cggccatcag atgggaatcg
    33601 ttggcaatcg ccagcaccac tttgatgtct tcttcttgca ggttgcgtaa ctctttacct
    33661 aacggccaca gtgctttgtc taccagacgt tgatcatagt attccgccaa ccacaggtct
    33721 gctttggcga agaccatctc cagcatgccg agacgcgtcg agaagaatgg ccaatcgcgg
    33781 cacatggctt ccagctcgct ctgtttgcca tcttcgacca ctttttgcag cgccgtacct
    33841 gcacccagcc aggcggggag catcaggcgg ttttgcgtcc aggcaaaaat ccacggtatg
    33901 gcgcgcagcg actcgacgcc gccggttggg cgacgtttcg ccggacgtga acccaacggc
    33961 agtttgccca attcttgttc cggcgtagcg gagcggaagt aaggcacaaa atctttgttt
    34021 tcacgtacgt agccacggta gagatcgcag gagatgactg acagttcatc cataatgcga
    34081 cgccagctct ctttcggctc aggtggtggc agcaggttgg cttccagaat cgccccggtg
    34141 taaagcgaca ggctgctgac ggtgatttct ggcagaccat atttaaagcg gatcatctcg
    34201 ccctgttcag tcacgcgcag gccacctttc aggcttcctg gcggttgtga cagcagcgcc
    34261 gcatgggcag gtgcgccgcc gcgaccaatg gaaccgccgc gaccgtggaa caacgtcagc
    34321 tcaatacccg ctttttcgca ggttttgatt aatgcatcct gtgcctgata ttgcgcccag
    34381 gaagctgcca tcacgcccgc atcttttgct gagtcggaat agccaatcat caccatctgt
    34441 ttgccctgaa tcaggccgcg ataccagtcg atattgagca gctgggtcat gacatcgttg
    34501 gcgttgttca ggtcatcgag ggtttcaaac agcggagcaa ccggcatcgc aaacccgata
    34561 cccgcttctt tcagcagcag gtggacagcc agtacgtcgg acggcgtttt cgccatcgag
    34621 atcacgtagg cggcaatgga gccttgcggc gcttcggcaa tcacctggca ggtatcgagc
    34681 acttcgcgcg tttcggcgct tggttgccag ttgcgcggca gaagcggacg tttggagttc
    34741 agttcgcgga tcaggaacgc ctgtttgtcg gcctctgacc agctttcgta gtcgccgata
    34801 ccgaggtagc gggtcagctc gcccagcgct tcggtatggc gggtgctctc ctgacggata
    34861 tcaatacgga ccagcggtac gccgaaacat ttcacgcggc gcagggtgtc gagcagatcg
    34921 ccgttggcga taatacccat gccacacgcc tgaagtgact ggtagcaagc gtagagcggt
    34981 tcccacagtt cttcgttttg tgtcagcagc ccttctggtt ttggcagttc ttcgcctttc
    35041 aggcgcgctt ccagccatgc ctgtgtcgcc atcaggcgag aacgcaggtt tttcatcaga
    35101 tagcgatacg gttctgcggc accttcttcg ccaaccagcg ccagcagttc aggggtcgct
    35161 tcaaccatcg acagttcaga aaccagaacc tgaatatctt tcaggaacag atcggtggct
    35221 ttccagcggc tgagcagcag gacgtggcgg gtgatatcgg cagtgacgtt cgggttgccg
    35281 tcgcggtcac cgcccatcca cgaggtaaaa cgtaccggaa caaattcgac gggcagtttg
    35341 tagtcgagat tctcttccag ttgttcgttc agttcgcgca ggtaattcgg tacgccttgc
    35401 cacaggctgt tttctactac ggcaaagccc catttggctt catctaccgg gcttggacgc
    35461 agcttacgga tttcatcggt atgccatgac tgggcgatca actggtgcag gcgacgcatc
    35521 agctggttgt gttcgtagtc agcgatatct ttgttatcga gctgttttaa acaggcgttc
    35581 acttccacca ttttgtggat cagtgtacga cgggtaattt cggttgggtg agccgtgagg
    35641 accagttcca gcgacagcga ttccactgct tttttgatgg tgtcttcgct cagttccggc
    35701 tggtttttca gtttacgcag ggtgcgggcg atcacttccg ggttgctggc agcttcgcct
    35761 ttcggcgaaa tgctgtggta ttgctcggcg gtgttggcca ggttcaggaa ctgactaaac
    35821 gcacgcgcaa cgggcagcag ctcgtcgttc gacaaatttt gtaaggtggt gagcaactcc
    35881 tggcggttag catcattgcc agcgcgtgaa gatttcgaca acttacggat agtttctacg
    35941 cgttcaagaa tgtgttctcc caacgcatcc ttgatggttt ctcccagcac tttgccgagc
    36001 atactgacat tactacgcaa tgcggaatat tgttcgttca tattacccca gacaccccat
    36061 cttatcgttt gatagccctg tatccttcac gtcgcattgg cgcgaatatg ctcgggcttt
    36121 gcttttcgtc gtcttttata aagccacgta aaagcggtga cgtcaaatgc tgcgaaatcg
    36181 cttcagcaaa cgaataaata gcaggaattt acgccattaa attcacgacg ctttaaataa
    36241 gcgtaactta tggaaatgtt aaaaaatcgc catacataac accaaaggtg taggtcggat
    36301 aagatgcgca agcatcgcat ccgacattat tgcggcaccg gagtttggca acggtgccgg
    36361 atgcggcgcg agcgccttat ccggcctacg ttttaatgcc agcaaaaatg gtgaattacc
    36421 tggataatca actcacgggt gggtttaata aaccgcgtct ccagatattc atcaggctga
    36481 tgagcttgat tgatggaacc cggaccgagc accagcgtcg ggcataacgt ttgaataaac
    36541 ggcgcttcgg tacagtaatt caccacttcg gtttttgctc cgagcaattt ctcaaccact
    36601 tcaaccagtt gatgattcgg tgggcattca tagccaggga tcggcggatg cagctcgtcg
    36661 accgtcagac gacccggcca gcgttcgctc accggagcca atgcatcgtt gagcaaacca
    36721 ttaagttcat tgagtgtcat gccaggctgc ggacgaatat ccatatgcaa ctcacagcaa
    36781 gcgcaaatac ggttagaagc gtcgccaccg tgaatatgcc cgaggttgag cgtagggtat
    36841 ggcacggtaa atgcttcgta gtgataacgt tctttcaggt tatcgcgcaa ttgcaaaata
    36901 tgcccgatgg cgtcgtgcat tagttcgata gcgttaactc cgcgtgctgg atcgctggag
    36961 tgccccgact ggccctgaat acggatggcg ttagagatat gacctttatg tgcgcgtacc
    37021 ggttgtagtg acgtcggttc gccaatgatg gcgcaatccg ggcgcagggc ggtagtttcg
    37081 gcaaaataac gcgctccggc catactggtt tcttcatcag cagtcgccag aatgtagagc
    37141 ggttttttca gtttcgtgac gtcgacatcg cgtagcgcat caaggataaa cgcaaaaaag
    37201 cctttcatgt cggcggtgcc taagccgtaa agcttgccgt catgctccgt cagtgtaaac
    37261 ggatcgcgcg tccagcgacc gtcatcaaat ggcaccgtat cggtatgccc cgccagcaac
    37321 aagccgccag ccccctgtcc gtaactggcc agcatattaa atttgttgcg agttcctgga
    37381 acaggctgca cttccacatt gaagcccaaa tctttaaacc agtccgccag cagagtgatt
    37441 aaatctgcat tgctttgatc gagtgcctct tccgtagcgc ttattgaagg tgtggcaatc
    37501 agagcgcggt aaatctcgat aaatggcggt aatttgtttt tcattgttga cacacctctg
    37561 gtcatgatag tatcaatatt catgcagtat ttatgaataa aaatacacta acgttgagcg
    37621 taataaaacc caccagccgt aaggtgaatg ttttacgttt aacctggcaa ccagacataa
    37681 gaaggtgaat agccccgatg ttgaatacgc tgattgtggg tgccagcggc tacgctggcg
    37741 cagagctagt gacctatgta aatcgccatc cgcatatgaa cataaccgct ttgactgttt
    37801 cagcgcaaag caatgatgcg ggaaagttaa tctccgattt gcatccgcag ctaaaaggca
    37861 tcgttgagct gccgttgcag ccgatgtcgg atatcagcga gtttagccca ggggtggacg
    37921 tagtgtttct cgccaccgcc catgaagtta gccacgattt agcgccgcag tttcttgaag
    37981 cgggctgcgt ggtgttcgac ctttccggcg cgtttcgtgt taacgacgcc accttctatg
    38041 aaaaatatta cggctttacc catcaatacc cggaactgct ggaacaggca gcctacggtc
    38101 tggcggagtg gtgcggtaat aaattaaaag aagcgaattt gattgcggtg ccaggctgtt
    38161 atccgacggc ggcacagctg gcgctgaaac cgttgattga tgccgatctt cttgacctca
    38221 atcagtggcc ggtgatcaac gccaccagcg gcgtgagcgg tgcaggacgt aaggcagcga
    38281 tttcaaatag tttttgtgaa gttagcctgc aaccgtatgg cgtctttact catcgccatc
    38341 aaccagagat cgccacacac ctcggtgctg acgttatctt caccccacat ctgggcaatt
    38401 tcccgcgcgg cattctcgaa accattacct gccgcctgaa atcgggtgtg acccaggcgc
    38461 aagtcgcgca agcgttacag caggcgtatg cccataaacc gctggtgcgg ctgtatgaca
    38521 aaggcgttcc ggcgctgaaa aatgtcgttg ggctgccatt ttgcgatatc gggtttgccg
    38581 ttcagggcga gcatctgatt attgtggcga ccgaagacaa cttactgaaa ggcgcggcgg
    38641 cacaagcggt acagtgcgcc aatattcgtt tcggctatgc ggaaacgcag tctcttattt
    38701 aagggtgcaa tgatgaatcc attaattatc aaactgggcg gcgtactgct ggatagtgaa
    38761 gaggcgctgg aacgtctgtt tagcgcattg gtgaattatc gtgagtcaca tcagcgtccg
    38821 ctggtgattg tgcacggcgg cggttgcgtg gtggatgagc tgatgaaagg gctgaatctg
    38881 ccggtgaaaa agaaaaacgg cctgcgggtg acgcctgctg atcagataga cattatcacc
    38941 ggagcactgg cgggaacggc aaataaaacc ctgttggcat gggcgaagaa acatcagatt
    39001 gcggccgtag gtttgtttct cggtgacggc gacagcgtca aagtgaccca gcttgatgaa
    39061 gagttaggtc atgttggact ggcgcagcca ggttcgccta agcttatcaa ctccttgctg
    39121 gagaacggtt atctgccggt ggtcagctcc attggcgtaa cagacgaagg gcaactgatg
    39181 aacgtcaatg ccgaccaggc ggcaacggcg ctggcggcaa cgctgggcgc ggatctgatt
    39241 ttgctctccg acgtcagcgg cattctcgac ggcaaagggc aacgcattgc cgaaatgacc
    39301 gccgcgaaag cagaacaact gattgagcag ggcattatta ctgacggcat gatagtgaaa
    39361 gtgaacgcgg cgctggatgc ggcccgcacg ctgggccgtc cggtagatat cgcttcttgg
    39421 cgttatgcgg agcagcttcc ggcactgttt aacggtatgc cgatgggtac gcggatttta
    39481 gcttaagttt tgttagccgg aggagcagct ttccggcatt gaatttcaaa ataaggaaac
    39541 agagttatgg cactttgggg cgggcgtttt acccaggcag cagatcaacg gttcaaacaa
    39601 ttcaacgact cactgcgctt tgattaccgt ctggcggagc aggatattgt tggctctgtg
    39661 gcctggtcca aagccttggt aacggtcggc gtgttaaccg cagaagagca ggcgcaactg
    39721 gaagaggcgc tgaacgtgtt gctggaagat gttcgcgcca ggccacaaca aatccttgaa
    39781 agcgacgccg aagatatcca tagctgggtg gaaggcaaac tgatcgacaa agtgggccag
    39841 ttaggcaaaa agctgcatac cgggcgtagc cgtaatgatc aggtagcgac tgacctgaaa
    39901 ctgtggtgca aagataccgt tagcgagtta ctgacggcta accggcagct gcaatcggcg
    39961 ctggtggaaa ccgcacaaaa caatcaggac gcggtaatgc caggttacac tcacctgcaa
    40021 cgcgcccagc cggtgacgtt cgcgcactgg tgcctggcct atgttgagat gctggcgcgt
    40081 gatgaaagcc gtttgcagga tgcgcttaag cgtctggatg tcagcccatt aggctgtggc
    40141 gcgctggcgg gaacggccta tgaaatcgac cgtgaacagt tagcaggctg gctgggcttt
    40201 gcttcggcga cccgtaacag tctggacagt gtttctgacc gtgaccatgt gttggaactg
    40261 ctttctgctg ccgctatcgg catggtgcat ctgtcgcgtt ttgctgaaga tctgattttc
    40321 tttaacaccg gcgaagcggg gtttgtggag ctttcggacc gcgtgacttc cggttcatca
    40381 ttaatgccgc agaagaaaaa cccggatgcg ctggagctga ttcgcggtaa atgcggccgg
    40441 gtgcagggtg cgttaaccgg catgatgatg acgctgaaag gtttgccgct ggcttacaac
    40501 aaagatatgc aggaagacaa agaaggtctg ttcgacgcgc tcgatacctg gctggactgc
    40561 ctgcatatgg cggcgctggt actggacggc attcaggtga aacgtccgcg ttgtcaggaa
    40621 gcggcgcagc aggggtatgc gaacgccact gaactggcgg attacctggt ggcgaaaggc
    40681 gtacctttcc gcgaggcgca ccatattgtg ggtgaagcgg tggtggaagc cattcgtcag
    40741 ggcaaaccgc tggaagatct gccactcagt gagttgcaga aattcagtca ggtgattggc
    40801 gaagatgtct atccgattct gtcgctacaa tcgtgcctcg aaaagcgtgc ggcaaaaggc
    40861 ggcgtctcac cgcagcaggt ggcgcaggcg attgcttttg cgcaggctcg gttagagtaa
    40921 tttgtttata ttgctcccct gaaaatgggg agtttaccac gcctacaaag tgcgacactt
    40981 cgtaggcgga taaggcgttt acgccgcatc aggcaga
//
LOCUS       DAJTXY010000019        38344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTXY010000019 DAJTXY010000000
VERSION     DAJTXY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.019"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..89)
                     /locus_tag="OO566_001163"
     CDS             complement(<1..89)
                     /locus_tag="OO566_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HCR6604528.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILIT"
     gene            372..422
                     /gene="hisL"
                     /locus_tag="OO566_001164"
     CDS             372..422
                     /gene="hisL"
                     /locus_tag="OO566_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6604529.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    398..521
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            568..1467
                     /gene="hisG"
                     /locus_tag="OO566_001165"
     CDS             568..1467
                     /gene="hisG"
                     /locus_tag="OO566_001165"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6604530.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1473..2777
                     /gene="hisD"
                     /locus_tag="OO566_001166"
     CDS             1473..2777
                     /gene="hisD"
                     /locus_tag="OO566_001166"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6604531.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2774..3844
                     /gene="hisC"
                     /locus_tag="OO566_001167"
     CDS             2774..3844
                     /gene="hisC"
                     /locus_tag="OO566_001167"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6604532.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3844..4911
                     /gene="hisB"
                     /locus_tag="OO566_001168"
     CDS             3844..4911
                     /gene="hisB"
                     /locus_tag="OO566_001168"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6604533.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4911..5501
                     /gene="hisH"
                     /locus_tag="OO566_001169"
     CDS             4911..5501
                     /gene="hisH"
                     /locus_tag="OO566_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6604534.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5501..6238
                     /gene="hisA"
                     /locus_tag="OO566_001170"
     CDS             5501..6238
                     /gene="hisA"
                     /locus_tag="OO566_001170"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6604535.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6220..6996
                     /gene="hisF"
                     /locus_tag="OO566_001171"
     CDS             6220..6996
                     /gene="hisF"
                     /locus_tag="OO566_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6604536.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6990..7601
                     /locus_tag="OO566_001172"
     CDS             6990..7601
                     /locus_tag="OO566_001172"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6604537.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7787..8764)
                     /gene="wzzB"
                     /locus_tag="OO566_001173"
     CDS             complement(7787..8764)
                     /gene="wzzB"
                     /locus_tag="OO566_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6604538.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8910..10076)
                     /gene="ugd"
                     /locus_tag="OO566_001174"
     CDS             complement(8910..10076)
                     /gene="ugd"
                     /locus_tag="OO566_001174"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR6604539.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEDYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10326..11732)
                     /gene="gndA"
                     /locus_tag="OO566_001175"
     CDS             complement(10326..11732)
                     /gene="gndA"
                     /locus_tag="OO566_001175"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6604540.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11841..12200)
                     /locus_tag="OO566_001176"
     CDS             complement(11841..12200)
                     /locus_tag="OO566_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604541.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12850..13413)
                     /locus_tag="OO566_001177"
     CDS             complement(12850..13413)
                     /locus_tag="OO566_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604542.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13621..14559)
                     /locus_tag="OO566_001178"
                     /pseudo
     CDS             complement(13621..14559)
                     /locus_tag="OO566_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14622..14996)
                     /locus_tag="OO566_001179"
     CDS             complement(14622..14996)
                     /locus_tag="OO566_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6604543.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFELLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15000..16148)
                     /gene="wzy"
                     /locus_tag="OO566_001180"
     CDS             complement(15000..16148)
                     /gene="wzy"
                     /locus_tag="OO566_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6604544.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16168..17073)
                     /gene="rfbG"
                     /locus_tag="OO566_001181"
     CDS             complement(16168..17073)
                     /gene="rfbG"
                     /locus_tag="OO566_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6604545.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17066..17956)
                     /locus_tag="OO566_001182"
     CDS             complement(17066..17956)
                     /locus_tag="OO566_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6604546.1"
                     /translation="MNSNIYAVIVTYNPELKNLNELITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17986..19242)
                     /gene="wzx"
                     /locus_tag="OO566_001183"
     CDS             complement(17986..19242)
                     /gene="wzx"
                     /locus_tag="OO566_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6604547.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19258..19803)
                     /gene="rfbC"
                     /locus_tag="OO566_001184"
     CDS             complement(19258..19803)
                     /gene="rfbC"
                     /locus_tag="OO566_001184"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6604548.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19808..20686)
                     /gene="rfbA"
                     /locus_tag="OO566_001185"
     CDS             complement(19808..20686)
                     /gene="rfbA"
                     /locus_tag="OO566_001185"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6604549.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20745..21644)
                     /gene="rfbD"
                     /locus_tag="OO566_001186"
     CDS             complement(20745..21644)
                     /gene="rfbD"
                     /locus_tag="OO566_001186"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6604550.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21644..22729)
                     /gene="rfbB"
                     /locus_tag="OO566_001187"
     CDS             complement(21644..22729)
                     /gene="rfbB"
                     /locus_tag="OO566_001187"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6604551.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23102..23995)
                     /gene="galF"
                     /locus_tag="OO566_001188"
     CDS             complement(23102..23995)
                     /gene="galF"
                     /locus_tag="OO566_001188"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6604552.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24170..25564)
                     /gene="wcaM"
                     /locus_tag="OO566_001189"
     CDS             complement(24170..25564)
                     /gene="wcaM"
                     /locus_tag="OO566_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6604553.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25575..26795)
                     /gene="wcaL"
                     /locus_tag="OO566_001190"
     CDS             complement(25575..26795)
                     /gene="wcaL"
                     /locus_tag="OO566_001190"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6604554.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSYEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26792..28072)
                     /gene="wcaK"
                     /locus_tag="OO566_001191"
     CDS             complement(26792..28072)
                     /gene="wcaK"
                     /locus_tag="OO566_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6604555.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRSYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMIADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28252..29730)
                     /gene="wzxC"
                     /locus_tag="OO566_001192"
     CDS             complement(28252..29730)
                     /gene="wzxC"
                     /locus_tag="OO566_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6604556.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVSVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29732..31126)
                     /gene="wcaJ"
                     /locus_tag="OO566_001193"
     CDS             complement(29732..31126)
                     /gene="wcaJ"
                     /locus_tag="OO566_001193"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6604557.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDTKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31181..32551)
                     /gene="cpsG"
                     /locus_tag="OO566_001194"
                     /gene_synonym="manB"
     CDS             complement(31181..32551)
                     /gene="cpsG"
                     /locus_tag="OO566_001194"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6604558.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32656..34092)
                     /gene="cpsB"
                     /locus_tag="OO566_001195"
     CDS             complement(32656..34092)
                     /gene="cpsB"
                     /locus_tag="OO566_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6604559.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASSEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGHHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34095..35318)
                     /gene="wcaI"
                     /locus_tag="OO566_001196"
     CDS             complement(34095..35318)
                     /gene="wcaI"
                     /locus_tag="OO566_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6604560.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLLFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35315..35794)
                     /gene="gmm"
                     /locus_tag="OO566_001197"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35315..35794)
                     /gene="gmm"
                     /locus_tag="OO566_001197"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6604561.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35794..36762)
                     /gene="fcl"
                     /locus_tag="OO566_001198"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35794..36762)
                     /gene="fcl"
                     /locus_tag="OO566_001198"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6604562.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36765..37886)
                     /gene="gmd"
                     /locus_tag="OO566_001199"
     CDS             complement(36765..37886)
                     /gene="gmd"
                     /locus_tag="OO566_001199"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6604563.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(37912..>38344)
                     /gene="wcaF"
                     /locus_tag="OO566_001200"
     CDS             complement(37912..>38344)
                     /gene="wcaF"
                     /locus_tag="OO566_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6604564.1"
                     /translation="YRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDD
                     VNLYTLGEITIGAHSVISQKSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAP
                     GVTIGDGTVVGARSSVFKSLPANVVCRGNPAVVIRERVETE"
BASE COUNT        10255 a         9585 c         8633 g         9871 t
ORIGIN      
        1 gtaatgagga tcggggcatg atcttcaacg gctttcatca ttgttgccga caaattctga
       61 cgcgcttcgc tgtagctaat tgtacgcatg taaatctcct attttgtaca gttcattgta
      121 caatgatagg tgttaattaa ctatttatta attagtttgt agatcaagac attgccagcg
      181 agacgaaaaa tcagactttg ctctttttgg tgctgtcaag ttagaggaca gtcctcttag
      241 ccccctcctt tccccgctca ttcattaaac aaatccatcg ccataaaata tataaaaaag
      301 cccttgcttt ctaacgtgaa agtggtttag gttaaaagac atcagttgaa taaacattca
      361 cagagatttt tatgacacgc gttcaattta aacaccacca tcatcaccat catcctgact
      421 agtctttcag gcgatgtgtg ctggaagaca ttcagatctt ccagtggtgc atgaacgcat
      481 gagaaagccc ccggaagatc accttccggg ggctttttta ttgcgcggtt gttaacggtt
      541 cagacaggtt taaagaggaa taagaaaatg acagacaaca ctcgtttacg catagctatg
      601 cagaaatccg gccgtttaag tgatgactca cgcgaattac tggcgcgctg tggcattaaa
      661 atcaatcttc acacccagcg cctgatcgcg atggcagaaa acatgccgat tgatattctg
      721 cgcgtgcgtg acgatgacat tcccggtctg gtaatggacg gcgtggtaga ccttgggatt
      781 atcggcgaaa acgtgctgga agaagagctg cttaaccgcc gcgcccaggg tgaagatccg
      841 cgctacttta ccctgcgtcg tctggatttc ggcggctgcc gcctttcgct ggcaacgccg
      901 gttgatgaag cctgggacgg tccgctctcc ttaaacggta aacgtatcgc cacctcttat
      961 cctcacctgc tcaagcgtta tctcgaccag aaaggtatct cttttaaatc ctgcttactg
     1021 aacggttctg tcgaagttgc gccgcgtgcc gggctggcgg atgcgatttg cgatctggtt
     1081 tccaccggtg ccacgctgga agctaacggc ctgcgcgaag tcgaagttat ctaccgctcg
     1141 aaagcctgcc tgatccagcg cgatggcgaa atggaagaat ctaaacagca actgatcgac
     1201 aagctactga cccgtattca gggtgtgatc caggcgcgcg aatcaaaata catcatgatg
     1261 cacgcaccga ccgaacgcct agatgaagtc atcgccctgc tgccaggtgc cgaacgccca
     1321 accattctgc cgctggcggg tgatcaacag cgcgtagcga tgcacatggt gagtagcgaa
     1381 accctgttct gggaaacgat ggaaaaactg aaagcactgg gtgccagttc aatcctggtc
     1441 ctgccgattg agaagatgat ggagtgatcg ccatgagctt taacacaatc attgactgga
     1501 atagctgtac tgcggagcaa caacgccagc tgttaatgcg cccggcgatc tccgcttctg
     1561 aaagcattac ccgcaccgtt aacgatattc tcgataacgt gaaaacgcgt ggcgatgagg
     1621 ccctgcgtga atacagcgcg aagtttgata aaaccacggt taccgcgctg aaggtgtctg
     1681 ctgatgagat cgccgccgcc agcgaacgcc tgagcgacga gctaaaacag gcgatggcgg
     1741 tggcagtaaa gagtattgaa accttccaca ctgcgcaaaa actgccgccg gtagatgtag
     1801 aaacgcagcc aggcgtgcgt tgccagcagg tcacgcgccc ggtggcttca gtgggtttgt
     1861 atattccggg cggctccgcc ccgctcttct caacggtatt aatgctggca actccggcgc
     1921 gtattgcggg ctgtaaaaaa gtggtgctgt gctcaccgcc gccgattgcc gatgaaattc
     1981 tttatgcggc gcagctgtgc gatgtgcagg acgtgtttaa cgtcggcggc gcacaggcca
     2041 ttgccgcgct ggcgtttggt acggaatctg tgccgaaagt ggacaaaatc ttcgggccgg
     2101 gtaacgcctt tgtcaccgaa gcaaaacgcc aggtaagcca gcgtctggac ggtgcggcga
     2161 tcgatatgcc cgcaggcccg tcggaagtgc tggtgattgc tgacagcggc gctacgccgg
     2221 attttgtggc ttctgatttg ctttctcagg ctgaacacgg ccctgactca caggtaattt
     2281 tactgacccc cgatgctgat atggcgcgtc gcgttgccga ggccgtcgaa cgccaactgg
     2341 cagaactgcc gcgtgccgaa accacccgcc aggcactgaa cgccagccgc ctgatcgtga
     2401 ctaaagattt agcgcagtgc gtggagatct ccaaccagta cggcccggag cacctgatca
     2461 ttcagacccg caacgcccgc gaactggtcg atggcatcac cagcgccggt tcggtatttc
     2521 ttggtgactg gtcaccggaa tcggcaggcg actacgcctc cggcaccaac cacgtgctgc
     2581 cgacttacgg ttacaccgcc acctgttcca gcctcgggct ggcagatttc cagaaacgca
     2641 tgaccgtaca ggaactgtcg aaagaaggtt tctccgcgct ggcttcgacc attgaaacac
     2701 tggccgccgc cgagcgcctg accgcccaca aaaatgccgt tactttgcgt gttaacgtcc
     2761 ttaaggagca agcatgagca ccgtgactat tactgattta gcgcgtgaaa acgtccgcaa
     2821 cctgacgccg tatcagtcgg cgcgtcgtct gggcggtaac ggcgacgtct ggctgaacgc
     2881 caacgaatat cccacagccg tggagtttaa gcttactcag caaacgctca accgctaccc
     2941 ggaatgccag ccaaaagcgg tgattgaaaa ttacgcgcaa tatgcaggcg taaaaccgga
     3001 acaggtgctg gtcagccgtg gtgcggacga aggtattgaa ctgctgatcc gcgctttttg
     3061 cgaaccgggt aaagacgcca tcctctactg cccgccaacg tacggcatgt acagcgtcag
     3121 cgctgaaacc attggtgtcg aatgccgcac agtgccgacg ctggaaaact ggcaactgga
     3181 cttacagggc atttccgaca agctggacgg cgtaaaagtg gtctatgttt gcagccccaa
     3241 caaccccacc gggcaactga tcaatccgca ggattttcgc accctgctgg agctaacccg
     3301 cggtaaggcg attgtggttg ccgatgaagc ttatatcgag ttttgcccgc aggcatcgtt
     3361 agctggctgg ctggcggaat atccgcacct ggcgattttg cgtacccttt cgaaagcctt
     3421 cgcgttggcg gggcttcgtt gcggatttac gctggcaaac gaagaagtca tcaacctgct
     3481 gatgaaagtg atccccccct acccgctctc gacgccggtt gccgacattg ccgcacaggc
     3541 gttaagcccg cagggaatcg tcgccatgcg cgaacgggtg gcgcaaatta ttacagaacg
     3601 cgaatacctg attgccgcac tgaaagagat cccctgcgtg gagcaggttt tcgactctga
     3661 aaccaactac attctggcgc gctttaaagc ctccagtgca gtgtttaaat ctttgtggga
     3721 tcagggcatt atcttacgtg atcagaataa acaaccctct ttaagcggct gcctgcgaat
     3781 taccgtcgga acccgtgaag aaagccagcg cgtcattgac gccttacgtg cggagcaagt
     3841 ttgatgagtc agaagtatct ttttatcgat cgcgatggaa ccctgattag cgaaccgccg
     3901 agtgattttc aggtggaccg ttttgacaaa ctcgcctttg aaccgggcgt gatcccggaa
     3961 ttgctgaagc tgcaaaaaga gggctacaag ctggtgatga tcactaatca ggatggtctg
     4021 ggaacacaaa gtttcccgca agcggatttt gatggcccgc acaacctgat gatgcagatc
     4081 ttcacctcgc aaggcgtgca gtttgatgaa gtgctgattt gtccgcacct gcccgccgat
     4141 gagtgcgact gccgtaagcc gaaagtaaaa ctggtggagg gttatctggc tgagcaagcg
     4201 atggatcgtg ccaacagtta tgtaattggc gatcgcgcga ccgacattca actggcggaa
     4261 aacatgggca ttaatggttt acgctacgac cgcgaaaccc tgaactggcc aatgattggc
     4321 gagcaactca ctaaacgaga ccgttacgcc catgtagtgc gcaacaccaa agagacgcag
     4381 attgacgtcc aggtgtggct ggatcgcgaa ggtggcagca agattaacac cggcgttggc
     4441 ttctttgatc acatgctgga tcagatcgcc acccacggcg gtttccgcat ggaaatcaat
     4501 gtcaaaggcg acctctatat cgacgatcac cacaccgtcg aagataccgg cctggcgctg
     4561 ggcgaagcgt taaaaattgc cctcggcgac aaacgcggta tttgccgctt tggttttgtg
     4621 ctgccgatgg acgaatgcct tgcccgctgc gcactggata tctctggtcg cccgcacctg
     4681 gaatataaag ccgagtttac ctaccagcgt gtgggcgatc tcagcactga aatgatcgaa
     4741 cacttcttcc gttcgctctc ttacaccatg ggcgtgacac tacacctgaa aaccaaaggt
     4801 aaaaacgatc atcaccgtgt agaaagcctg ttcaaagcct ttggtcgtac cctgcgccag
     4861 gccatccgcg tcgaaggcga caccctgccc tcgtcgaaag gagtgctgta atgaacgtgg
     4921 tgatccttga tactggctgc gccaacctga actctgtgaa gtctgccatt gcgcgccacg
     4981 gttatgaacc caaagtcagc cgtgacccgg acattgtgtt gctggccgat aaactgtttt
     5041 tacccggcgt cggcaccgca caagcagcga tggatcaggt gcgtgagcgc gagctgtttg
     5101 atctcatcaa agcctgtacc caaccggtgc tgggtatctg cttagggatg caactcctgg
     5161 gacgacgcag cgaagagagc aatggcgtcg acttgctggg catcatcgac gaagacgtgc
     5221 cgaaaatgac cgactttggt ctgccactgc cacatatggg ctggaaccgc gtttatccgc
     5281 aggcaggcaa ccgcctgttt caggggattg aagacggcgc gtacttttac tttgttcaca
     5341 gctacgcaat gccagtcaat ccgtggacca tcgcccagtg taattacggc gaaccgttca
     5401 ccgcggcggt acaaaaagat aacttctacg gcgtgcagtt ccacccggag cgttctggtg
     5461 ccgctggcgc taagttgctg aaaaacttcc tggagatgtg atgattattc cggcattaga
     5521 tttaatcgac ggcaccgtgg tgcgtctcca tcagggcgat tacggcaaac aacgcgatta
     5581 cggtaacgac ccgctgccgc gcttacagga ttacgctgcg caaggtgccg aagtgttgca
     5641 cctggtggat ctgaccgggg caaaagatcc ggctaagcgt caaatcccgc tgattaaaac
     5701 cctggtcgcg ggcgttaacg tcccggtgca ggttggcggc ggtgttcgta gtgaagaaga
     5761 cgtggcggcg ttactggaag ctggcgttgc gcgcgtggtg gtcggttcca ccgcagtgaa
     5821 atcaccggac gtagtgaaag gctggtttga acgtttcggt gccgatgcgc tggtgctggc
     5881 actggatgtc cgtattgacg agcaaggcaa caagcaagtg gcagtcagcg gctggcaaga
     5941 gaactcgggc atttcactgg aacaactggt ggaaacctat ctgcccgtcg gcctgaaaca
     6001 tgtgttgtgt accgatatct cgcgcgacgg cacgctggcg ggatctaacg tctctttata
     6061 tgaagaagtg tgtgccagat atccgcaagt ggcgtttcag tcctccggcg gcattggcga
     6121 cattaacgat gttgctgccc tacgtggcac tggcgtacgc ggcgtaatag ttggtcgtgc
     6181 attactggaa ggtaaattca ccgtgaagga ggccatcgca tgctggcaaa acgcataatc
     6241 ccgtgtctcg acgttcgtga tggtcaggtg gtgaaaggcg tacagtttcg caaccatgaa
     6301 atcattggcg atatcgtgcc actggcaaaa cgctacgccg aagaaggtgc agatgaactg
     6361 gtgttctacg atatcaccgc ttccagcgat ggccgtgtgg tagataaaag ctgggtatct
     6421 cgcgtggcgg aagtgatcga cattccgttt tgtgtggcgg gtgggattaa gtctctggaa
     6481 gatgccgcga aaattctttc ctttggcgcg gataaaattt ccatcaactc ccctgcgctg
     6541 gcagacccaa cattaattac tcgcctggcc gatcgctttg gcgtgcagtg tattgtggtt
     6601 ggtattgata cctggtacga cgccgaaacc ggtaaatatc atgtaaatca atataccggc
     6661 gatgaaagcc gcacccgcgt cactcaatgg gaaacgctcg actgggtaga ggaggtgcaa
     6721 aaacgcggcg ccggagaaat cgtcctcaac atgatgaatc aggacggcgt gtgtaacggt
     6781 tacgacctca agcagctgaa aaaagtgcgt gaagtttgcc acgtcccgct gattgcctcc
     6841 ggtggcgcgg gcaccatgga acacttcctg gaagccttcc gcgatgccga cgttgacggt
     6901 gcgctggcag cttccgtatt ccacaaacaa ataatcaata ttggcgaatt aaaagcgtac
     6961 ctggcaacac agggcgtgga gatcaggata tgttaacaga acaacaacgt cgcgaactgg
     7021 actgggaaaa aaccgacggt ctgatgccgg tgattgtgca acacgcggta tctggcgaag
     7081 tgttaatgct gggctacatg aacccggaag ccttagacaa aaccatcgaa agcggtaaag
     7141 tcaccttctt ctcgcgcact aaacagcgac tgtggaccaa aggcgaaacc tcgggcaatt
     7201 ttctcaatgt ggtaagtatt gccccggact gcgacaacga cacattactg gtgctggcga
     7261 atcccatcgg gccaacctgc cacaaaggca ccagtagctg cttcggcaac accgctcacc
     7321 agtggctgtt cctgtatcaa ctggaacaac tgctcgccga acgtaaatat gccgacccgg
     7381 aaacctccta caccgccaaa ctgtatgcca gcggcactaa acgcattgcg cagaaagtgg
     7441 gcgaagaagg cgtggaaacc gcactggcag caacggtaca tgatcgcttt gagctgacca
     7501 acgaggcatc tgacttgatg tatcacctgc tggtgctgtt gcaggatcag gatctggatt
     7561 taacgacagt aattgagaac ctgcataaac ggcatcagtg agttacgggc tgagcaggtg
     7621 tgatgttgtt gtcacacccg gagccattgc ccgatgcgca aacttatcgt gcctacacat
     7681 tatctcaaca cacgtaggcc ggataagatg cgccagcatc gcatccggca aaaaaaacgg
     7741 gcatggtgtc accaccctgc ccgtttctct taaatgcaca ataatattac ttcgcgttgt
     7801 aattacgcag agcgttacgc cccaacacaa tccccgcgcc aaccatgccg cccagaagca
     7861 ctgccagaat caaggtgatt gcctttttcg gactatcgcg acgaataggt aacgtcggtt
     7921 tcatcacata gcggtaagca tgaatatcaa gatcatcaaa ctttaatttt tcaatatcca
     7981 acaggttttg acgtgtctga tagtagtttg gtgagaacac caacggacga gtcgcttcat
     8041 gcttaatcat cgactccagc gcttcgctcc ctagaaggaa caacgtatct tgcgtcacat
     8101 cttcagtctg ctgaacctgt ggctttgtca cctgcgcctg attcgcatac tgcaacgctt
     8161 cctgaatctg acggatacgc agatctttct gctcctgcgc gaccacttcc tgggttctta
     8221 aagagtcctg caagtttttc cgtcccagag caatgttgtc cttgagatcc ttttctagct
     8281 cttgattcac tttatcatca acttgctgaa tgtattgggc caacttcatt tgtgcgccct
     8341 ctgcagtttg cccaacataa gaaacagtca atggtaattg ttggttctta acagaaggtt
     8401 cgatggtaag tttttctggc tcttcctgat tatccagcgt ttctgctaat gcagagaagg
     8461 cagaactgaa gcgaccaatt aacgtctcct gcaaatccga tactttcggt gcagcctgac
     8521 catagataac attcatggca ttgttatagc cagcaatttg ccccacgtcg ggctgagtga
     8581 taattgctgt tgacgtccat ttctccttcg ctactgccaa ataaccaata gccagggcaa
     8641 tagccacaat gacggaaatg ataattgtca tcttgccacg ccacaactgc actagtaaat
     8701 caatcaaatc aatctgttcc gggtcatggt tttgcccaga aacattatta ttttctactc
     8761 tcatcattac cctaactgac gaaaatttcc aagtccgcat agtttagcga taatccccgt
     8821 gaatgctata ggataagtga taaaaataat gaattattgc ggaggtaaca aaagacaatc
     8881 agggcgtaaa tagccctgat aacaagatgt tagtcgctgc caaagagatc gcgggtgtag
     8941 accttatctg ccacatcctt aagctcttct gccatacggt tggagataat gacgtcggct
     9001 tgttgtttga aggtggcgag atcacgttcc aggcgagagt tgaagaatga gtcttctttc
     9061 atcaccggct cgtagatgat cacttcaacg cctttcgcct tgatacgctt cataatcccc
     9121 tgaatagaag acgtacggaa gttatctgac ccgctcttca taatcagacg ataaataccc
     9181 accacttgcg gtttgcgtga caaaatggca tcggcaataa aatctttacg cgtgcggttt
     9241 gcatcgacaa ttgccgagat caggttattc ggcacagact ggtagttcgc cagtaactgc
     9301 ttggtatctt tcggcagaca ataaccacca taaccaaacg acggattgtt gtaatggttg
     9361 ccaatacgcg gatcgagaca aacgccttcg ataatctggc gagtattcag acctaaactt
     9421 tctgcataac tatccagttc attaaagtat gctacgcgca tcgccagata ggtattagcg
     9481 aaaagtttaa tcgcttctgc ttcagtggag tcggtaaaca gggtcgggat attttgctta
     9541 atcgcgcctt cctgtaacaa cgcagcaaaa cgttctgcgc gttctgaacg ctcaccgatg
     9601 acaatacgtg acggatggag attatcgtaa agggctttac cttcgcggag gaattccgga
     9661 gagaaaataa tattttcagt acgatatttc ttatgcatcg ctgcggtaaa accaacggga
     9721 accgttgatt tgatgaccat aaccgcataa ggatttatct caactacgtc tttaattact
     9781 gattctacac tggatgtatt gaaataatta gttttaggat catagtcggt tggagtggcg
     9841 atgatgacat aatcagcatc ccggtagtct tcatttttat ctaatgtggc attaaagtgt
     9901 attttatctg attgcaaaaa ctgctgaatt tccttatcaa caataggaga tatccgatca
     9961 ttcagcatag caacgcgtga cggtaaaata tctaatgcca caacctcatg attttgtgcg
    10021 attagaagcc cgtttgacaa gcctacatag ccagtaccgg aaatggtgat tttcatttca
    10081 tgctctcaga attaacttaa ctgtgaatca tgatgttttt agcatcgtga taagatgcta
    10141 aaagttttaa cgctacgttg tcagatggtc aacgcaacga aaaacaattc agattttctt
    10201 tataagaata gctgatgttt attaagttaa tattaagcag tgaaatttag tctataagat
    10261 atttggcaaa aaaaagcccg gtgcaatata cgccgggcct caattttatt gttggttaaa
    10321 tcagattaat ccagccattc agtatggaac acaccttctt tatcaatgcg cttataagta
    10381 tgcgccccga aatagtcacg ctgtgcctgg atcaggttcg caggcagaac cgcggcacgg
    10441 tagctatcgt aataggcaac cgcagcggcg aaggtcggaa ccgggatacc gttctgtact
    10501 gcataagcga cgacatcgcg cagcgcctgc tggtagtcat cggcaatttg cttgaagtac
    10561 ggtgccagca gcaggttagc gatctgcgga ttttcggcat aagcatcggt gattttctgc
    10621 aggaactgcg cacggatgat gcagccagca cggaaaatct tcgcgatttc gccgtagttc
    10681 agatcccagt tgtattcttc agacgcagca cgcagctgag agaagccctg agcgtaagaa
    10741 acgattttgc ccagatacag cgcactgcga actttttcga tgaactcagc cttgtcgcct
    10801 gctgactgag cttgcggacc agagagaact ttagatgcgg caacgcgctg atctttcaga
    10861 gaagagatat aacgtgcaaa cacagactcg gtaatcagcg acagcggttc gccgagatcc
    10921 agcgcgctct ggctggtcca tttaccggtg cctttgtttg ctgcttcatc cagaatcaca
    10981 tcaaccaggt agttaccgtc ttcatctttt ttggtgaaga tatctttagt aatgtcgatc
    11041 aggtagctgc taagttcacc gttattccac tcggtgaaag tctgggccag ttcttcatta
    11101 gatagattca ggccaccttt gagcagagaa tacgcttcag caatgagctg catatctccg
    11161 tattcaatac cattgtgaac catcttcaca taatggcctg cgccgtcggc accaatataa
    11221 gtaacgcatg gctcgccgtc ttctgccacg gcagcgattt tagtcaggat aggcgcaacc
    11281 agctcataag cctctttctg cccgccaggc ataatagaag gacctttcag cgcgccctct
    11341 tcaccgccgg aaacaccggt accgatgaag ttaaagccct ctgctgaaag ctcacgatta
    11401 cgacgaatgg tgtcctggaa gaaggtatta ccaccatcaa tgatgatgtc acctttatcg
    11461 aggtatggtt tcagggaatc aatcgcagca tccgtgcctg cacctgcttt caccattaac
    11521 aggatgcgac gaggcgtttc cagagattca acaaactctt tcaccgtata gtaaggagcc
    11581 agtttcttgc ctggattttc agcaatcact tcttccgtct tttcgcggga gcggttgaaa
    11641 atagagacgg tataaccacg actttcgatg ttgagcgcaa ggttgcgccc catcactgcc
    11701 ataccgacta cgccgatctg ttgctttgac attgtttact cctgtcttct atattattaa
    11761 ccgtctatta accgttatat tattaaccgt cgtgattttc atcttctaat caaggaatac
    11821 atatggttac agcatgcttc ttactttata gcgataagta tgccattggg tgtatccata
    11881 agaactttga attttggata cttttctgga ttaaaattag catagcttcg tttagatata
    11941 acaataaacg aactattaaa agatttcatc ttctctatta aatcatcctg tgggattacc
    12001 tcacactcgg gattactaat agtccaatca ctatatcttg ctaatccaaa gacgtcacca
    12061 attgccatcc ctttatagaa gtaaatacca tcctccaagc ctaattggat aagattatct
    12121 ccatataact cactatattc acttgctttc ttcatcagtt catagctata gcgactttct
    12181 aagcgtgaat taaaattcaa gtattcagaa taaaaatatt tgaattcaaa atgcaacgat
    12241 cgccccgaaa aaaaaataat acacacaagg ataaaatatc gtcccaactt gtaatttgta
    12301 atgttgatat cttttattaa acttgcaatt gagatcgcaa ccagcaatac tgacaatggg
    12361 taaatgggtg ataaatacct gtcaccttgc gtaacagaaa accaaaataa gacatatgtt
    12421 aaaacaatcg agaaaaatac ccaaagtatt ttattattcc taagtgtaaa gacagacagt
    12481 atagcataaa aaacaacatc actgccaact ttattaattg cttgttgcaa atctaatgtg
    12541 gcaggactta taccatgtcg agcttgttct gctgtttgaa cttgtagatc aatttcattc
    12601 cataggtagt gaccaaatat atttcctcca gcaggactaa aaggatcacc agagatccaa
    12661 aaactacgga cataccaata aatgccacca agaattgttg ccaagctaaa aataagaaat
    12721 gtctgtatgt ttctcttttt cgctatcaat ataacaaata tgatggctcc atatatggca
    12781 ccaaatagtt ttacgccaca agcaattcca ataaaaaaac cacataaaaa aattaattca
    12841 ttttgtgttt tattgttttc tgagcgaata tatataagaa tactggctgc agcaaaacaa
    12901 agatagcgtt aaatcaatat atgcataacc aagatgatct ttaagtggat tgatagtaaa
    12961 tagtaggatt gtagcaataa tgctaggata ataagttttc gtaatttccc atgaacagcc
    13021 aacaagtcca caggctatta caaaaaaaca gatatttgct aaaaactgag ctaaccttac
    13081 atctcctaac tgtaaaccta aagccattaa aagatctgca ttttgcggga acagaggaaa
    13141 acgcaaatat tgttcaacaa caattgcatg gtgctcaact aatgaacgag ccaaaggtaa
    13201 gtgatacata gtatcatccc aatcaccggg agctcgcagg gacgataaca atgtaaccac
    13261 aaaaatcaaa acaagaaata aaataacaaa cagcgtactg aactcaatat tatttattgc
    13321 cagccatcta aattgctgac gtttaataat gcttaaaacc caacacaccg taaaaattaa
    13381 aaagatagca atcttattaa attgcccgac catgcccaga aagtaaatta tcaaaatatt
    13441 taaagacatc cctatgcata ggcaataaaa aaataaagaa aatttgttaa gtcctgccac
    13501 cccctcctta aaaaaagatg acaatacaat attaccaatg acaaaaaatg tcattccaaa
    13561 agcaaaacaa tacacaacat acaaatacaa cggcaattca atcatagtaa aatactctat
    13621 ttacattatc agtttatagt ttttttcaga aagataaaat aatgccttca tttcacgtct
    13681 agttgttgta tttgaatgca aaataatgcc acatacaaat gccattattg acgataacat
    13741 gatggaggca gttaaaaagg cagtcggaaa tcttctcacc aaacctgtat caatataatc
    13801 aacaatgatg ggcaatccta aaattataga caaagcagca agtaaaacgc cgataatact
    13861 aaaaaagtat agtggccgtt caagcgagta aagtttaatt attgttttca gaatcttgaa
    13921 cccatcactc caagttgata gtttgctcac actcccttca gatcgctcgc catattttgt
    13981 attaacctca ccatacttca tacgtaattc taaagcatga attgtaagct cagtttcgat
    14041 ttcaaatccc cttgatagac atggaaaaga tttaacatag cgtctggaaa atacacgata
    14101 ccccgacaac atatcagtaa aattcccttt aaagatagtc tgaacagtac cagtcaacag
    14161 tttatttcct cagcgatgcc cttttctgta tgtattttga tctccattct cttgacgaca
    14221 tcctacaacc atatccaatt gattttttat gagatggtta atcatttctg gagcagaact
    14281 tgcatcataa gtgccatctc catcaaccat tagataaata tccgcatcta catctgagaa
    14341 catacgtctt acgacattcc ccttgccttt cagaggaaca gagtgaactg ttgctccagc
    14401 atcctctgcc acttgtgctg tactgtcagt cgaattatta tcaaatacat atatagaagc
    14461 agttggaata ttcgttttga aatcatttat gacttttgct attgccttct gctcattata
    14521 acaaggaata attacagcga tattaaaaga gtgatacata agcccctcat ataaactatt
    14581 tagctaatgt gcaagtaatt gtgtttttaa caaatcgttt actaacgaaa aatgaaatat
    14641 tttgatgcgc aatatcctaa aaccaaacta aaaagagaaa aggtgaacaa tgtaaatatt
    14701 ggagataaag ctaagagttc aaataaaacg ccgaataaat aactcatcgc ccccataaaa
    14761 aatataaata taaaatatct agccttggtt gcagatactt taaatgagca tcttgcattt
    14821 gcaaaaaaag aaaaggttgc tgcacatata aaaccaacaa tgttacttcg gccttgacca
    14881 aaagctaaat tattataaca cgcataaaat accccccaat gtaaagcagt attgacaatt
    14941 ccaattaaaa aataactaaa aaaagaactg gtcaataact tccctatttt taacatcctt
    15001 tattttgctc cagaagtgag gttattacta atttggatat tttctataga aaatacccat
    15061 aaaactacaa cgcctataat aaaaattccc atgggtgtaa aaagaaatgg attagttata
    15121 gaaacggtaa tactgaaaaa aagtattaac gacatcatta atgatgtttt tgtactattt
    15181 aaaaaatagc ctttcaaaaa gataagcaac ataggaacta atgatataaa tacaccaata
    15241 acgcctgttt tctgaagtat ctcaagaggt actatttcaa tccttaatcg atctaatata
    15301 aatgaaccaa atcctcttcc aaacaagaac gtcgctaaat caacattttt ataataaaaa
    15361 tataaatcat taaatctaac agagtcagaa tcttcactgc gtgatccgac catgtaaagc
    15421 ataattataa atagaacgct gcccaatatt attataatag tatttaaata tagcttatgt
    15481 aatttaaaac tggctaataa cagtgataat gttgtaaaaa gtaatacacc tcttgattct
    15541 gtcaataata tcgcaagcac tattatgaga cattttaaat attttttgtc gacaaaactg
    15601 ataatcgcac cgacaccaaa aaagtagaag gatttggaaa aaaaagcccc atcgggacga
    15661 aaaaagaact cgctagtcaa tgataagtgt tcataaatta aattaaaatt taacaaaccg
    15721 aaattcaaca aaattatgta tgaaatatat aacagtgatc catataatgc aaatgcatta
    15781 actattttaa taaaaatctc acataaaaat acttttaaag tatatcttag tgtaaataga
    15841 tatataagtg acagcataaa aaggtatggt tgcacatcaa acagtatatc atttatttta
    15901 ttaccagcta ataagccgac aaagaagcca taaaaaggaa ataaaagcaa ccatataaaa
    15961 agactgacac atttttgggt gattgaaaaa ttgaatgtaa ctaaattaaa aattaaaagt
    16021 ataaaactaa atacaaataa taaatatcgc aaagggaata ttcccaatgg ctccagtaat
    16081 cttccaccac caccaataat cagttcaata cataaaaatg ttataaaaat tttatttata
    16141 ttattcatat tcgtaaggtg atgtttttta aaatatgttt aaaatcaaca ttaataaaat
    16201 ataaatttta tcagcctttt tccgccttaa cttccaaaaa cttaaagcca agatcaaatt
    16261 gcctatataa cctttaccca attgattatt cacaaagcta tcatcagcta aggcattagc
    16321 tattttagtt acttcttttc gataccactt gtttctcacc aaaccaagcc gtttgtattt
    16381 tgctttaaaa ctaatgtttg ctcccacttg gttgttttca tgctgtctgt acagcattgt
    16441 tggttttcta tcaatatacc acgaataatt tctcgtccgc gcaaatgaat ataaaaacca
    16501 atcatgcaga cagatatctt gtgcagcatt tttattattt atgatgaact tcttaaattc
    16561 aataagcgtt tcttttttta taacatatgt acaccccgga cctgctgctt caaaaaaata
    16621 atcgaattga gtttgcgact gagccttatc aactaaattt ttttgccctg atggataata
    16681 tgcaattaca ttgctggaat aacaattcgc attattaaat accaagcagt caattgcatt
    16741 ttttatttta tctttatacc aaatatcgtc ctgatcacta aaacagatat aatcatagtt
    16801 ttcaagatcg acatctttaa ttaatctata aaaattttta cctgcccccc caaacttatt
    16861 gacgcttgga agtatattta taattgaggg atatgataat tgaaattctt cacaaatatt
    16921 taatgtatta tcagttgata aatcatctga tataaaaatt gagatgtcta catctttttg
    16981 ttcaataata ctttttaatt gttcctcaat ccagcattcg ccattatacg tagccataag
    17041 tacagctact cttttttgat cagaactatg cattataacg accgccccca gtaattcctc
    17101 ttattccatc cagcgtagcc aaaatatgtt ttttcatata ttcaatttta tttttttgag
    17161 ttgcgataat taaagtcgta tgaattaaag aaaaaacaac ttccctaata gagagtaaaa
    17221 gaggcacatg attttttcta agcaaaagaa atgcatttct tactcgataa taacgacgta
    17281 ctggcgagtg aattggtatg ttaatcccac agatttttac tgacttatcc ccaatagcat
    17341 gaaccatttc tatatcagga atgatatgaa ctaatatgcc atagtttaag catctaagac
    17401 accattctgt atcgacataa tcaataaaaa gttcctcatc catcattcca acaatctcaa
    17461 aaacttcttt tgaaaccatg gttccggatg agattgttac tgaactttta aacggtgtct
    17521 gtccttcagt gacatgaatt ttctctcgaa gaccattttt tttgatatta caaataggat
    17581 aataatgtga cttatctcta tcaataaaaa ctgggccagc tatttttgca ttttcattat
    17641 ccatatagca ggaaagtttt ttgataaact cattgcgaat acggctatct tgatcaaaaa
    17701 aaattatctt ctctgcccct ttttcaagag aatatcgaat cccaatattt tgtgcttttg
    17761 ctataccttc attcctgcca agacaaatta aatgaacttt ttcaatgtct gctaatttat
    17821 ctttcaaggt gaaattagtc ctattatcga ctactacaac gtaacaattt tgctccttta
    17881 gttcagtgat cagttcattc agatttttaa gttcgggatt atatgtcaca atgacagcgt
    17941 aaatattact attcattatc caagtgactc agtaattggt taattttagt tttcatatac
    18001 agaacacatt ctagtataaa gccattgtaa taacagagca tctaaaatta cacgtatgct
    18061 ccaggatatt gcggcaccca gtaacccata tttttcgacc ataaaataca aaagggctaa
    18121 ataaggaacc aattcagcac aatgaatcaa agcagtaatt ttagattttc ctaatgcctg
    18181 gattgcagag aatggtatca ttgccagcgc attaaagaaa aaaccgatca ataacacatt
    18241 taatatatta aaggaattaa tttggtaatt ctcaccaaac catattttca atacaagccc
    18301 tgaatataac aagcctatga ttatcaccgg caaacagatt agaaacatta aaagcaatga
    18361 tttatttaca ttacgtttaa attctttaaa gtctttgatg ttacttaacc ttggaaacac
    18421 tgccctcccg attgctgcag gtattattcc taattttaga ataacctccg ctggtgcaga
    18481 ataaaatgca actttatctg cccccataat atttgacact ataaatcgat caaaatacac
    18541 catgaccgga cttatgatat tacttactgt catccaaccg ccaaagaaaa agagtcgttt
    18601 aaaggtttta taacaaaaat gaacccccga aattttaata tcattcctga cataataggc
    18661 agagactaaa atcgaaataa ctcttgcaaa tattaaacca gcaaccgctg ccgacaatgt
    18721 agcactataa aaaacaaata tggccggaat tcccgcgatg caagagcttg atatagattt
    18781 ttgaatattt acaatgccaa atttttcatc cccctcaaga atggctgacc ataattgatt
    18841 tagaataaat aatggaatgc aaatagccaa cagtttaaat gctagttgta tatcactatg
    18901 ttcaacacca gaaattttta gataattaac aattccatca gagaaaatca ataataaaaa
    18961 agcaccaaag catgaaaata gcaccaaaaa agatgtactt gttgaaatta ccttggttct
    19021 ttcatgatga ttatcacgat gaattgcaat ttctctaata acagagcgag tcaggcctac
    19081 atcaaaaatt ccagcatacc caacaagtgc aattgcaatt gtataaacac cgaaaccttc
    19141 aggccccaaa cctcgagcga gaaatcctag cgaggggata gcaattaaag ttggtattgc
    19201 atagccaaaa aggttccaga cactattttt tattatgctc attattacat ttctttatca
    19261 tataaaataa tcagcatcta caaatttttg accattaata tctttatcag aaagcaatgg
    19321 attttgaacc ggccattcta tatttatact tttgtcgctc cataaaatcc cacgctcttg
    19381 catttttgaa tagaaattat ttgttttgta gacaaattct gcttcatcac taagaacgca
    19441 aaacccatga gcgaatcctt ccggtatcca taattgacgc ttattctccg ccgacaaatt
    19501 tacaccaacc cacttgccaa atgttggtga tgatttccga atatcaacag ctacgtcaaa
    19561 aacttcaccg acaacacagc gtaccagttt tccttgagca taaggttcca gctgatagtg
    19621 cagtccgcgt aacacaccct tagttgattt agaatggtta tcctgaacaa actcaacctt
    19681 tcgtcctaca gcctcttcga aaactttctg attgaagctt tccataaaaa agccccgctc
    19741 atcaccaaat acttttggtt caaaaattag cacatcagga atttcagttt taattacgtt
    19801 cattttatta ataaccttta atcattttca gcagatactg accataagca tttttcttca
    19861 gcggctcggc taataccttc acttgctcag catcaataaa gcctttacgg taagcaattt
    19921 cttccgggca ggaaactttc agcccctgac gctcttcaat tgttgcaatg aagttgcttg
    19981 cttcaataag actttgatgt gtccccgtgt ccagccatgc ataaccacgc cccatcatag
    20041 cgacagacaa acgtccttgg tccatataaa tacggttaat atcggtaatt tccagttcgc
    20101 cacgtgcaga tggtttaagg tttttcgcca tttctacaac gtcattgtca tagaaataaa
    20161 gcccagtaac cgcatagtta ctttttggtt ccagcggttt ttcttccaga ctaattgccg
    20221 taccgttatc atcaaattcc accacaccgt agcgctcagg atcatttacg tgataggcaa
    20281 aaaccgttgc gccactctct ctgttaacag cggtatccat taatttcggc aggtcgtgac
    20341 catagaagat attatcacca agtaccagcg cacaatcatc accacctata aattcttcac
    20401 caatgataaa tgcttgcgcc agaccatccg gactcggttg tactttatat tgcagattaa
    20461 gcccccactg gcttccatca ccaagcaatt gttgaaaacg tggtgtatcc tgcggcgtac
    20521 tgataatcag aatatctcga atacccgcca acataagcgt tgaaagcgga taataaatca
    20581 tcggtttatc ataaatcggt agcagttgtt tgctcacagc cattgtcaca ggataaagac
    20641 gagtgccgga accaccagcc agaataatac ctttacgcgt tttcatttca ctattccttt
    20701 taattcatct cgctctggca tcatgagcga gatgcaataa tttgttaaat tgccgtggtc
    20761 gtaaataatt cgttgagcat acgtttcacg cccacctgcc agtcaggcaa gacaagcgca
    20821 aagttctgct gaaatttttc ggtattgagg cgagaattat ggggacgacg tgctggtgtg
    20881 ggataggccg ttgtcggcac ggcattaagt ttgttaagag caaggttgat ccccgctctg
    20941 cgcgcttctt caaataccag agcggcataa tcgtgccagg ttgttgtacc accagcaacc
    21001 agatggtaca aaccagcaac ttctggttta tttgcggcca cgcgaatggc atgagcggtg
    21061 caatcagcca gtaattcagc ccccgttggt gcgccgaatt gatcgtttat tacagccaac
    21121 tcttcgcgtt cttttgccag gcgcaacatc gttttggcaa agttatttcc tttaccagcg
    21181 tatacccagc tggtacggaa aataagatgc tttgcgcaat gttcttgtaa cgctttttcc
    21241 ccagctagct tggtttcacc gtagacattt agcggtgcgg ttgcatccgt ttccagccat
    21301 ggcgtgtcac cgttgcctgg gaaaacataa tcagtggagt agtgtataac ccaggctccg
    21361 acttcattag ctgcttttgc aatcgcttcg acgcatgtcg cgttaagtaa ttgcgcgaaa
    21421 ttcggttctg attccgcttt gtctactgca gtgtgagcag cagcattaac aataacatcg
    21481 gggcgaattt ttttgacggt ttcagccaca ccttcggggt tgctgaaatc tccacaataa
    21541 tcagtggagt gaacatcgag cgcaatcaag ttacccagag gagccagagc acgctgcagt
    21601 tcccaaccca cctgccctgt tttgccgaaa agcaggatat tcattactga cggccctcat
    21661 agttttgttc aatccacgac tgataagcac cgcttttcac attctcaacc caatttgtat
    21721 tagccagata ccattccacc gttttacgaa tcccgctctc aaacgtttct tgcggtttcc
    21781 agcccaattc gcggctaatt ttatcggcat caatggcgta acggcggtca tgccccgggc
    21841 gatcggcaac ataagtaatt tgctcacgat aagatttctc tttcggtaca atctcatcca
    21901 acaaatcaca aatagtgagc actacgtcga tgtttttctt ttcgttatgt ccaccaatgt
    21961 tataagtttc acccgcttta ccttcggtta cgacggtata taacgcacga gcatgatcct
    22021 ctacatacaa ccagtcgcga atttgatccc ctttaccata aataggtaat gccttacctt
    22081 ccagagcatt cagaataacc aatggaataa gtttttccgg gaagtgataa ggaccataat
    22141 tgttcgagca attagtcaca atggttggta aaccataagt ccgcttccag gcacgtacca
    22201 aatgatcgct ggaagcttta gacgcagaat acgggctact tggcgcgtaa gctgtcgttt
    22261 ccgtaaatag cggtaacgct tcgttattat ttgcttcatc cggatggggt aagtcaccat
    22321 aaacttcatc agtagaaata tgatgaaagc ggaaactttt tttcttttca tcattcagag
    22381 cagaccagta attgcgcgcc gcttctaaaa gaacataagt ccccacaata ttggtttcaa
    22441 taaatgccgc agggcccgtt attgaacggt caacgtggct ctctgccgcc aggtgcatca
    22501 ccgcgtctgg ctggtgctgt gcgaaaatac gcgacatggc tacagcatcg cagatatctg
    22561 catgctcaaa agcataacgt tcagaatcag aaacatcagc gagtgattct aggttcccgg
    22621 cgtatgttaa tttatcgaca ttaacaacac tatcttgcgt attatttatt atgtgacgaa
    22681 caacagcaga accaataaac cctgcgccac cagtaacaag tatcttcact tttttattcc
    22741 atatagctat agagcatgct gtgaaataga ctgctccaga tttgattaat agaggtatta
    22801 atacacgcta ccgcccctgg cttaacagct accagtgcac tgcatgcatc tcaacgatgt
    22861 ggcgagcgtt agccactcgc gccaaatccg aaaaattcaa acgttaattg tcttaccaaa
    22921 cagccgtaga aacaaggaaa atcctggaaa aatttgaata atatgagcta tgtaacttct
    22981 tgtttttata ggctttataa aaacggcaac ggcaggaaaa ttcaatgagc aaaacttatg
    23041 ccacgaatct tcactgccgt tataattttc ttatcaaccg ttacatccgg tcagattttc
    23101 attattcgct taacagcttc tcaatacctt tacggaactt cgccccttct ttcaggttgc
    23161 gtaggccata cttcacaaac gcctgcatat agcccatttt tttgccgcag tcgtaactgt
    23221 cgccggtcat cagcattgca tcaacggatt gttttttcgc cagctcggca atagcatcag
    23281 tcagctgaat acgtccccat gcaccaggct gagtacgttc cagttccggc caaatatcgg
    23341 cagaaagcac atagcgacct acggccatga tgtctgagtc cagcgtctgc ggctgatccg
    23401 gtttttcgat aaattcaaca atacggctga ctttaccctc acggtccagc ggctctttag
    23461 tctggatgac ggagtattca gagaggtcac ccggcatacg ttttgccagc acctggctgc
    23521 ggcccgtttc gttgaaacgt gcaatcatgg cagcaaggtt gtaacgtagc gggtcggcgc
    23581 tggcatcgtc gatcacaacg tctggcagta ccacgacaaa tgggttgtca ccaatggcag
    23641 gtcgcgcaca caaaatggag tggcctaaac ctaaaggttc gccctgacgc acgttcataa
    23701 tggtcacgcc cggcggacag atggactgta cttccgccag cagttgacgc ttcacgcgct
    23761 gctcaaggag tgattctaac tcataagagg tgtcgaagtg gttttcgacc gcgttcttgg
    23821 acgcgtgagt taccaggagg atttctttga tccctgcagc cacaatctcg tcaacaatgt
    23881 actgaatcat tggtttgtcg acgattggta gcatctcttt gggtatcgcc ttagtggcag
    23941 gcaacatatg catcccgaga cccgctacag gaataactgc ttttaaattc gtcattattt
    24001 catccacctg taaaatggtt gctgaattat agcttgttcg atttttttcg ccagcatcaa
    24061 ttaccctgaa ttgattactg aattacttgt gatgttacgc cgcttcgttg tggattgcag
    24121 tagcattgtt cctaagtatg gctccatttt tccaggaatg gtcgcaaatc tactccctcc
    24181 gttccggcaa tctaaagtta atcttttcca cattaacaat atggtgatta atcctgtcga
    24241 tatcgacgga gctttgccct ctttcattca ccgcatgaac atttgcaaga gacagcagtg
    24301 tttctttttt cgccataaag acgccgcgaa cgtctttgcg catgtcgaag ttcatgctca
    24361 atgctggtcc aactgaggat tcctgcatca cattgatatt tcgtataaaa agatgttgcg
    24421 gcttgttgtg taactccagc gaggcacgct tcatctcaat gttagttagc gacacaaagg
    24481 agacggcatt cccggcggag atttggatgc cgcgcaattt ataagcaaga tgggtattat
    24541 ccagttgaat attattcact cggaaatttt gcggtatcga gagatatttg cctttaatta
    24601 ccccatagcc gattaacatc ccggcgctat taatcatttc aatattatca atcacgaaat
    24661 tgtcacaacc gtaaatagcg actgtcgcgt tatcaatgcc cgctttctta ctgaaatccg
    24721 gcgtgatatt gcgggcattg atattacgaa taacaaaatg tttaccattt tcaacatggt
    24781 tcaactgccg acaatccgat cccgtgatat tcgccacgac aaagtttttc actgcctggt
    24841 cttccgggta gttgttatca taagtgcttc ccgcaaggcc tatgccgatg ccccagttga
    24901 ttttgccgtt ggtacagttg atacgctcga tgacatggtc agatatcaaa atatcactgt
    24961 cgttaattgc cacgttccat tcaatggcgt cgccctgtaa gtcgctgaac ttacagttgg
    25021 taatgttggc accgataatc tggttatgaa atccctggcg taagatggcg taattagcgt
    25081 ggctgacagt gaggttatcg atggtcaggt tgcgcatgac ccgtttgttt ttgccgccaa
    25141 tataaatctg cattaccggg ccaaagccgc tcatcgccag ccctttgatg gtgcagtcag
    25201 aaccacgcac atccagggta atgttatgca taccgccgcc ctctccccct gtcacctggc
    25261 tgccgtcctg taagacaaat cgccctctgc cgttgccgcg caggcttcca aggatgtgta
    25321 acgttttgcc gggagggatg aagatgccgg tgttgatatt gtcacaaacg aatccggcag
    25381 gcacgacgac cgtttgccct tcgctgaagg cttgtttaaa tgaggcgatc cagtcatgtg
    25441 ggttgtagtc gttaatgtta acgctttgtc gggcgggaag tgcgcgagcg aaaggggtat
    25501 ggaggaaggc aagcgccgag cttgccgtca ggaaggtgcg tcgggagagt gttttaaatg
    25561 gcatacgttc tcctctataa agcctgcagc aagctggcga gttctcgatt aatcacctgc
    25621 tggttaaaat cgtgttcgac tttttcgcgc gcacgtttga cgaccggagc cagttcgtcg
    25681 gtatccagtt ggctaaacgc cgccagacgt tgtgccagtg cgcgagcatc gttctcaggc
    25741 accagccagc cggatttatc ggcctccacc agttcaggaa ttccgctatg cagagtagaa
    25801 accaccggaa taccgaccgc catcgcttcc attagcgcca ccggaatgcc ttccatatcg
    25861 ccatccgcgc ccgttaccga tggcaacagg aagacatccg cgtcgtcgag catctccttc
    25921 acttcatagc tcggtttaaa gcccggcatc tctaccacat cttccagttg atattgttcg
    25981 atgagggtgc gcaggcgtcg ttcccacagg ccaatgccga ggatgcgata gcgaaatgcc
    26041 acgccctgct ctttcaactg acggcaggct tcaatcgcca catgcaggcc ttttttctcg
    26101 gttaagcgtg cgacagatat gatttccagc ggcgttgcgg gcgctttcac cggacgcggg
    26161 ctaaagcgcg tcatgtccac gcccatacgc gatacggcga ttttttctct ggggcagccc
    26221 attttttgca gccttccggc ccacagatcg cttatcggta acatcaggtc gccacggcga
    26281 aacagttgtt gatattcggg agtgtagtgg ttgagcactt cccgactgga gatatcaata
    26341 ccgtggaaga tggtggcaat tttgccgcga atgataccca gttcgcgtag ttttgctgcg
    26401 gttacccccg ccgggccaaa atgagcgatg aacacatcgg cgcgaaacgg tgttgcgacc
    26461 tgaccacaaa tggcagacaa aatcaggttc cgcgactcgg caccatagcg tttgaggttg
    26521 agcgcatgcc aggtattttt acgatgaatg ccacgtaagg tctggctggc gcggtggcgc
    26581 agtttcgcta ctttgcctgc aggttcgtcc tgtaaccagc gggttctggc gacaaggttg
    26641 tatttcgtcc atgccgcgtg ggtgttctct gtatcgcctt tttgcaaagc gacaatctcc
    26701 acttcaaagc ccatatcaat aaacgcggta atttgattga ggacgaaggt ttccgacgac
    26761 agcgggaatt tcaataagaa gaagccgacc ttcatttcac ctccccgatg cgctcaagca
    26821 cagactgcac catctgcatt cccgtctgac gttcgcgact gacggcttcg ttaaatcgag
    26881 cgttcagcac cggaagctgg cctaaggtat ccgcaatcat cgcttgcagg ctgccgtcca
    26941 gtaaatgacg gatatcaatc gccatctctg gcagtcccag ctgctgcata atcccggcgg
    27001 atttatgttc atagttgatg gcaatcgccg gagtagcaaa gttcattgag ataatggcgg
    27061 agtgcaggcg cgtaccgacg gtgagttcac aggctccgag aattttgccc atttccagat
    27121 cgttgagttc atccatcact acgtggtaac gggcaggatc gctgatgtgc tggcgcagat
    27181 tgagcgccac catgcggtcg tctttgttat agctgtcgat acccgtacag gtggagagcg
    27241 caatcacctg atagccttca tcgagaatgc gattgaccac cccggcaaag gctttttcat
    27301 acgcttgttg agtggtgccg agacgtttgt cgaacggtgc cagttcgcgc agggtaattg
    27361 ccactgtttt ctgttgtgcg gcaacgtcca gccagtgttg aacggcatag ctggcggtga
    27421 agtcttcggt gtggtgatcg accagccacg cggtatcgac gccatgttcc acttttgcgg
    27481 tggtgatatt gctgcgtttc atcaaatcaa ggctgaccga ttcgcgcagg atcagcgcgt
    27541 cgcagtgacc aaagacatag ttcgccagtt ggttaaattg ctcatcctgg aaggggccga
    27601 cgctgtgacc aatcataaac agcggctttt tcgccataaa tgtgcaaagc gcatgttcaa
    27661 actgcggcac gccgtagaga tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt
    27721 aacctgacag caggcgcacg aagtcggtga atccctgggc gatggcgata ttgcgcagct
    27781 tgccagtgtc ggtgacgcgt gagagcaata cctgatgctg gtagctgcga cggaggactt
    27841 ttttaacgcg cccgacaacg cccgccgcgc tattgtgttg tgtcatctgc aggaacagcg
    27901 gatcgcccat taccgggcgg ttgagcagcc aggaagaact gaccggatag cggctcatca
    27961 cgtccacttc ggtatgtgga ttgaggatgt tgatggcatc aagtaagccg cgcaagatgg
    28021 cgctgtcgcc acgattgccg caagtgtggt tgcccagaat aagtaatttc attttttcct
    28081 cataaatttg atgccaggtg aggctgtgtt tgttgtgttc gttgccgttt tttgcctgat
    28141 gcgacgctgg cgcgtcttat caggcctaca ggtcccgagc acagaaccgt aggacggata
    28201 aggcgtttta cgccgcatcc ggcaaccgtt gtcggaaccg aaaacagcaa ttcaccccgc
    28261 ccgtaaaagc attttcattt tttcgctgcg acaaaactga cgcttcactt ccaccaccag
    28321 cggatggcgt gacagcacaa tcatcaccac aaacgccagc acgcccgcgg ctatttgcac
    28381 cgccagcagc atccccagcg ccagttgccc tttcagcaca atgcccagcg cataactgac
    28441 caccagcgtc ggcaacgaga gataaaacgg cagccacaaa ctcaggatgt actggcgata
    28501 actggaacca agaaccggtt taatcatcac gaagtaactc agaatggtgt tgataatctg
    28561 caccagcagg aaaccgagcg tgacgccgat tgcgcccgcc atctgcccac caataacaat
    28621 cgccggaata aacagaaatg ttttgaatac attgaattta aagctgatat cgacccgcgc
    28681 tttcgccatc agcagcgaac caatcgggtt acccacggag cgcagcagac ccacaataca
    28741 cagcaattgc agcaccggaa taatgctgtt ccacttttcg ccgaatacca gcggtacgaa
    28801 attattcgac accaccatca gccccagcag cgccggaaag ttgataatcc ccactatcga
    28861 cagcagcttg tagaagttaa cacgcagctt ttcggtgtcg tcctgaattt tggcaaacgc
    28921 cggaaacaac acgcgggtga tgattgggtt cagcttcatc ggcggtacga cggccacgtt
    28981 gtacgccagg ttgtatcccc ctgccacgcc cgcgccaaga atacgcgcca gcaccagcgt
    29041 tgaaaggttg gtgttgagat agttgatgat gctgtccgcc gtcagccagg caccaaaacg
    29101 taagttcggt gccacagacg ccagcgagaa gtgcagaccg gggcaataaa ttttgcgtcc
    29161 aaagtagcca aacagcagtg ttctcaccgc actattgacc agataaccga ggatcgcggt
    29221 catcgccagc ggccagaaat gggcgctaac taccgtaaaa gtgaagcccg ccagcaccgc
    29281 gctggtttcg atcatgccga ttttgttgaa ctccagctcc ttctgcatca acgcgcggaa
    29341 ctgttgcccg tgggggatca ccacaaacgc cagcgacaat gttttaatca acggtgccag
    29401 gtccgggtta ttcagcacgt cgccgatgac atcactcaac aaaaacaccg acacacatac
    29461 cacaagcccc agcccgacgt tcagccagta cagcgtggtg agttcaaggt gactgatttc
    29521 ttttcgctga ataatcgagt tagcgatacc gaagtcagaa agcgtatctg ccagcgcgat
    29581 aatcaccagc gacacagtca gcaggccgaa ctggtggttg tcgataatcc gcgccagcac
    29641 ggtcatctgc accagcccga ggccgatgat gatcaccgtg gcaatagccg accacttcgc
    29701 gccgctgatg gttttttcac gtaagctcat atcaatatgc cgctttgtta acgaagccct
    29761 tgaataccgt caggaaaacg attttgatat cgaaccagac gctccattcg cggatgtact
    29821 caaggtcgaa ctcgacgcgt ttttccattt tctccagcgt gtcggtttcg ccgcgccagc
    29881 cattaatctg cgcccagccg gtaatgcccg gtttcacttt atggcgcagc atgtagcctt
    29941 caatgagctg gcgatactgt tcgttatgcg ctactgcgtg cggacgtgga ccgacaatcg
    30001 acatcccccc ggtcagcaca ttgataaact gcggcaattc gtccagcgag gtgcggcgca
    30061 gaaagttccc cactttagtg acgcgcggat cgttctgcgt cgcctgggtc accactttgt
    30121 cgttctccat cactttcatg gaacggaact tccacacttt gatcggcttg ccatccatac
    30181 catagcgagt ctggcggaaa ataaccggcc ctggtgaact gagtttcacc gccagcgcaa
    30241 tacagcacag caccggggag atcagcagca gaataagcgt cgccagcaca atgtcttccg
    30301 cacgttttag caggcggtta ccccccgaaa gcggcgtgtc atacagcggc accaccggaa
    30361 cgccgttcat ctcttcgaga cgtgaatgga gaatgttgaa ggtaaagacg tcggggatca
    30421 gcagcaccga acaggtggta tccgccagtt gatgggccag ttttttcact cgtgcgccgt
    30481 cgcacatttg catcgcgata tagacgttat gaatcttgcc cgcttttgca tcctcgacca
    30541 gctgttgcag gttacccgcc cagtcgttag aaacgccgcc cggtttcgtg tcgtggtaaa
    30601 cgcccaccac ttcaaaccct aaccacggct gattacggaa actctccatc agcatttgtc
    30661 cggcggctaa atcccccgct acggcgacca tccgcttgtt gtagccatga ttacgcagcc
    30721 agcccgcccc aatgcgaata catgaacggc aaaccaccag tccgatgctg gtcagcccat
    30781 accacgccag ccagattttc agttgcgtgt cgaaatcatt gttgaacgcc accagtccgg
    30841 cgctgaaaat cacgcttaag gtccagtttt gtagcaacag ggcaaattct gtcgctgccc
    30901 gaacaccgcg ccatgagcga taaaaatcgg tgatgccgcc cagcatctgg aacaccacca
    30961 gcgtaatcag cgccaccaac aggtgcatgt agaggaatga cagtccgctg acttcgcaaa
    31021 ccagccatag cccggcaaac atgatggtga tatctgaaaa gcgttgcacc atagagatta
    31081 acgatgcatt ggttttcgct cgctcgcgct tttttagatt tgtcatcgtt gttcctgtta
    31141 ttagcccctt acccgcagtg gggtaaggga agatccgaca ttactcgttc agcaacgtca
    31201 gcagagttcg cgttcgcgct tccatcagcg gcacatcacc gcgcgattcc acattcaggc
    31261 gcaccaccgg ttcggtattg gaggtgcgca ggttaaagcg ccagtcggca aaggtcatgc
    31321 tgatgccatc gctgcgatcc accgccagcg cctcacggct aaaatgctgt tccacgcggt
    31381 taatcgcctc aacgggttgc gccagtttgc tgttgatctc accgcttgcc ggaaacgccg
    31441 ccatccggtc gcgcaccagt tcgcccagcg tttttccttt caggcacacc agttcggcga
    31501 ccagcagcca cgggatcatg ccgctgtcgc agtaagcgaa atcacggaag taatggtggg
    31561 cgctcatttc gccaccgtag atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt
    31621 gtccggtttt cgacattacc ggcgtgccgc ctgcagcagt caccacatca atagtgttcc
    31681 aggagagacg cggatcatgg atgatcttcg cgccgggatt tttttcgagg aacgcttctg
    31741 ccagcaggcc gacaatgtag tagccctcga taaactgccc tttttcgtca aacagaaaac
    31801 agcggtcaaa atcgccatca aaggcaatgc ccatatccgc gccgtgtttg atgaccgcat
    31861 tacgggtgtc gtcgcgacat tccggcagca gcgggttagg aataccgttg gggaaattgc
    31921 cgtccggtgt gttgtgtact ttgattaatt ccacgggcgc gccgagggct ttaaagcggg
    31981 cttcaatggc gtccaccacc ggacccgctg cgccattccc ggagttgatc accagcttga
    32041 gcggcgtgag atttttgaca ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca
    32101 ggttgatttg ctgatagcga ccgcgtttgg tttcatcgac gggaggaaag tcgttggctt
    32161 cagccagacg ctggacgtcg cgcagtccgg tatcaccgct gatcgggcga gccccctcgc
    32221 gcaccagctt catgccgttg taatccatcg gattatggct ggcagtaact tcaatgccgc
    32281 catccacgcc gagatggaac gtggcgaaat agatctcttc ggtgccggac ataccgatat
    32341 ccagcacatc gacgcccgca tcctgtaaac cttttgccag cgccagtttt aaggtttcgc
    32401 tggtgagacg gacatcaccg cctaacacaa tggttttcgg tttgagaaat tcgccatagg
    32461 cgcgaccaat gcgccaggcg atatcttcat tcagttcttc gcctaatttc ccgcgaatat
    32521 cataggcttt aaagcaggtt aattttttca tgtcgttacc ctttttcagg caatagttgg
    32581 ccctgaccga aacggccaat tttatttttg tcgttattta caccgcggtt tcgcattcat
    32641 tgcctgatgc gacgtttaca cccgtccgta gcgatccgcg aagcgcacca catcatcctc
    32701 ttcgagataa gagccggagc gcacttcaat taaatcgagc ggaattttcc ccgggttttc
    32761 caggcagtgc gtcgccccca gcggaatata aatggactcg ttttcaccaa gcagtttgat
    32821 atcaccatca atggtgactt ttgccgttcc cgcgaccact acccagtgtt ccgcgcggtg
    32881 atggtgcatc tgtaccgaca aaccctcgcc cggtttcacg gtgatgcgtt tcacctggta
    32941 gcggtcgccc gcgtcgatag agtcatattt gccccacgga cgatacactt cgcgatgcac
    33001 ccgatgctca tggtgaccat cggctttgat ctgctcgacc acttttttca catcctgtac
    33061 cgcgttacgg tcggcaatca gcaccgcatc tttggtctgc accactacca gatctttcac
    33121 cccgacggtg gtgactaggc ctgattcggc atacacatag ctgttttcag ttttgtgatt
    33181 aatcacatcg ccgtggcaaa cgttgccctc ggcggtgtgg gcgctgatct cccataatga
    33241 agaccaggaa ccaacatcgc tccagcccgc atccatcggc accacaacag catctgccgt
    33301 acgttccatg accgcgtaat ccaccgactc ttccgggcag gcgagaaacg cttcttcatc
    33361 cacacgaata aaatcgagat ccggatcgac ggcgctcatc gctttttcgc aggcatcgag
    33421 gatatccggg cgatattttt tcagttcttc gagatagcgt ccggcgcgga acaggaacat
    33481 gccgctgttc cagtaatatt cgctgcttgc tacataggcc tgggcggttt ccagattcgg
    33541 tttttcgaca aactgcgcca cttcaaaggc caccgtatcc tgctcacccg cagacacttc
    33601 accgcgacga atatagccat aaccggtttc tggtagatcc ggcacaatgc caaaggtcac
    33661 cagcttgccc gcttcggcat aaggcatggc attacgcacg gcggcacgga acgcgtcttt
    33721 atcggcaatc acatgatccg ccgccaatac cagcattaac gggtcgctct ccgggctatg
    33781 acgttttgcc gccagcgccg ccagtgcaat ggcaggtgca gtgttacgcc ctgccggttc
    33841 gagaataatg ttctcggtga gtttgttcag ttgacgcagc tgttccgcga caataaagcg
    33901 gtgctgctca ttgcaaatca ccaccgggct ttcgcactcc acgccgttca ggcggcagat
    33961 ggtggtttgc agcatggtga gatcgccttt caggcataaa aactgcttgg ggtaaagtac
    34021 gcgggaaagc ggccataaac ggctaccgga gccacctgcc atcacaactg gatagagttt
    34081 cgactgcgcc ataattatcc ccgaatatca tttataaatt gacgtaacac gttctcttta
    34141 tcgagcgtgc gttcggcata ttcacgtgcc accgtgttat gtttgggcag caggagcgcc
    34201 tgacgaatcc ccgccaccag cgcctcgacc gattccggtt ctacgcaaac cgcaataccc
    34261 ggaaaggttt cgcaaagctg ccccagttct gtgtgggcct ctgcggtaat caccgcgtta
    34321 ccgcctaccg ccagaatatt ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt
    34381 ttttgcacca ccagatggca atcgcccatc ttcagcagtg cgggtaaagc gtcatacgat
    34441 tgcagcggga aaaattgcat gttgcgcagt ccacgctgct gcgccatttt ttccagccgc
    34501 gctttgccgc cgccctgccc gacaatggca aaaagcagcg gttcatcgcg caggagatcg
    34561 gcagcttcaa taacgttttc cagcccctgc ttttcaccaa tattgccgga gtaaagaatg
    34621 atttttttgt tatccggcag gccaagctgg ttacgaaggg catcaacatc ggcgtctgca
    34681 acatgctgaa aacgggcaat ttccgaccag ttggggaaga agatgacgtt atccgccgcc
    34741 acgccttttt cgatggcttt attcatcatc gaacgcgaaa tcgtggaaac gttatcgacg
    34801 ttatgcagtc cgctacgttc gaacgccgtt gccagctgtg ccactttgcc gcctttgcct
    34861 tttccggcaa ggcccagccc cagcatggcg tccacttcgt aatcctgaat atgcagcacg
    34921 gtacgcgcac cagagagctt cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc
    34981 actacgccaa taatgcgatc cggcttccag cgacgttgcg ccatcagtgg aaagaaactg
    35041 ctgacggcaa aactgccgag atgcaacaag cgtttcaggg tgctcggctg ttttggcacg
    35101 tacagcgggc agcgccacac cgtggcggcc ccttcttctc gtttgtagcg ccaggcggaa
    35161 tagttctcac ccacctgcca ttgcgggtag taaggcggtg cggtaatgac ccgcacctca
    35221 tgaccttgtg ccgccaacca ttccaccatc tcgccggtgt atttgccgat gccggttaac
    35281 tccggcgagt agttaatgcc gtagaccagt attttcataa tccgggtact ccggcacgct
    35341 tctcagcgag aaaataggcg cggctgttag cgtgaacatc gttactggcg agcagcgcgt
    35401 ccggcgtcag ccagcggtaa tcgtcatgct gctcatccgg cagtaacagc tcttcttccg
    35461 ctactctgaa gcgaaaaccg agcaccacat agtgagtggt gaaatccgtg ccggagaagt
    35521 tatcgtcata aaaatgctgc cagacaccgt aaaactggcc tgctgttatc ggcagacgca
    35581 gccccagttc cgccatcgtc agccgctcaa atgcggcttc cagcgtttcg tctttctgca
    35641 cgcgccctcc cggcacaaac cagtaaccct gcgccgggcg gttggttctt ttgccaagca
    35701 gaaactcgcc gcgactgttc tcgacaataa agtcgagaga gacaagcggt gtggagcgca
    35761 ctacagtggc aaagtcttcc tgacgtaaaa acatcattcc ccccgaaagc ggtcttgatt
    35821 ctcaaggaac cactggtaag tgctggcaag ccccgcttcc agtgagattt cgtgatacca
    35881 gccaagctga tgcaggcgcg tcacatccag cagtttgcgc ggcgtaccat ccggtttgct
    35941 ggcatcaaaa accacccgac ctttgtaacc caccactttg gcgatggttt gcgccagctc
    36001 gcggatagtg cagtcaacgc ccgtgccgac gttaatgtgc gacagcatcg gttgggtatt
    36061 ctccagccag acttcatgcg ccagctccat gacatgaatg ctcgccgccg ccatatcatc
    36121 gacgtgcagg aattcacgca tcggtgtacc gctgccccat accaccacgt ccggcgcatt
    36181 ctgtgccgtc gcctcgtgga agcgacgcag caatgctggt atcacatggg agttactcgg
    36241 gtggaagttg tcatgcggcc cgtacaggtt ggtcggcatg acagagcggt aatcgcgtcc
    36301 atactggcgg ttgtaagatt cgcacagttt gatcccggcg attttggcaa tggcgtaagg
    36361 ctcgttggtc ggctccagcg tgccctgcaa taactcgctt tctgccatcg gctgttttgc
    36421 cagtttcggg tagatgcagg acgatccgag aaacatcagt ttgttcacgt cgttctgatg
    36481 cgcggcgtga atgatgttgc tctcaatcat catgttctgg tagatgaaat ccgccggata
    36541 ggtgttgttg gcaacaatgc cgcccacttt cgccgccgcc agatagacct ggtcaatgcg
    36601 ttcgctggca aagaaatcat gcacggcgcg gctgtccagc aggttcagtt catcgcgggt
    36661 acgtaatacc agctccacat caccgcgctg ttcgagctgc cgcttgatgg cagaaccgac
    36721 catcccacaa tggccagcga taaaaatgcg ttgtttactt atgcttatga ctccagcgcg
    36781 atcgccacgt cgtagccgtg agatttcagc agagagtgtt ttttcgccgc ttcgaggtca
    36841 ttagccacca tttcagacac catctctctg agggtgattt ccggtttcca gcccagtttt
    36901 tcgtgcgctt tggtcgggtc gccgagcagc gtttcaactt ctgccggacg gaagtaacgc
    36961 ggatcaacgg cgataatcac atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg
    37021 gaaaccacaa tgcccttctc ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc
    37081 tgtgctgccg ccatttccac aaactgacgt acggagtact gaacgccggt cgcgataacg
    37141 aaatcttccg gctgttcctg ttgcagcatc atccactgca tttttacgta gtctttggcg
    37201 tggccccagt cacgcaggga atccatattg ccgaggtaca ggcacgactc cagcccctgg
    37261 gcgatgttgg cgattgcgcg ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg
    37321 gattcatggt tgaagagaat tccgttacaa gcgtacatgc cgtaggattc gcggtagtta
    37381 acggtgatcc agtaggcgta cagtttggcg accgcatacg gagatcgcgg gtagaacggc
    37441 gtggtttctt tctgcggaat ttcctgcacc agaccataca gttcagaggt ggaagcctga
    37501 tagaaacgag ttttcttttc cagaccgagg aagcggatcg cctccagcag gcgcagcgta
    37561 cccatcgcgt cgacgtcagc ggtatattct ggtgactcaa aagagaccgc aacgtggctc
    37621 attgcgccca ggttgtacac ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta
    37681 gaggtatcac tcaggtcgcc ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc
    37741 tgataaatgt gatccacgcg ctcggtgttg aatgacgatg cgcgacgctt aataccatgc
    37801 acctcgtaac ctttttccag cagaaactct gccaggtaag aaccgtcttg tccggttaca
    37861 ccggtgatga gagcgacttt tgacatgtct tattcctctg tatttttgaa tttattcagt
    37921 ttcaacgcgt tcgcgtatca ccactgcggg attcccccgg caaaccacat ttgccggaag
    37981 cgatttaaaa acactgcttc gtgcacccac gacggtgccg tcgccgattg tgacgcctgg
    38041 ggcaacaaag acatcggttg ccagccagca tttctcgcca atcacaatag gcgtggcgtt
    38101 aatggtgaaa tgttgacttg catggtcgtg gctaccggtg cataaataac ttttttgcga
    38161 tatcaccgaa tgtgcgccaa tggttatttc accgagggta tataaattga cgtcatcgcc
    38221 aacccacgcg taatcaccta aggttaattt ccacggatag gtaattttta ctgacggacg
    38281 aataactacg ttttttccta tttttgctcc gaataaacgt aataaaaaag cccgccagcg
    38341 atac
//
LOCUS       DAJTXY010000020        37707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTXY010000020 DAJTXY010000000
VERSION     DAJTXY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.020"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..1995
                     /gene="speF"
                     /locus_tag="OO566_001201"
     CDS             <1..1995
                     /gene="speF"
                     /locus_tag="OO566_001201"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR6604565.1"
                     /translation="RVPAEYLPRISGVFEHCESRKEFYGRQLETAASHYETQLRPPFF
                     RALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLCNADVAMGDLL
                     IHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNH
                     HGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRAKEARPFRLA
                     VIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLNEND
                     PGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLF
                     AALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYET
                     AQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPYKLLLTTPGIDARNGEYEAFGVPAT
                     ILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVL
                     PSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHVSMNPQEANYA
                     YLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGCAVLRYFSALEEGINLLP
                     GFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            1992..3311
                     /gene="potE"
                     /locus_tag="OO566_001202"
     CDS             1992..3311
                     /gene="potE"
                     /locus_tag="OO566_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6604566.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSITMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3367..5007)
                     /gene="pgm"
                     /locus_tag="OO566_001203"
     CDS             complement(3367..5007)
                     /gene="pgm"
                     /locus_tag="OO566_001203"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6604567.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5033..5578)
                     /gene="seqA"
                     /locus_tag="OO566_001204"
     CDS             complement(5033..5578)
                     /gene="seqA"
                     /locus_tag="OO566_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6604568.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5763..6527
                     /gene="ybfF"
                     /locus_tag="OO566_001205"
     CDS             5763..6527
                     /gene="ybfF"
                     /locus_tag="OO566_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6604569.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6667..6960
                     /gene="ybfE"
                     /locus_tag="OO566_001206"
     CDS             6667..6960
                     /gene="ybfE"
                     /locus_tag="OO566_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6604570.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7100..7630
                     /gene="fldA"
                     /locus_tag="OO566_001207"
     CDS             7100..7630
                     /gene="fldA"
                     /locus_tag="OO566_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6604571.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7840..7926
                     /gene="uof"
                     /locus_tag="OO566_001208"
     CDS             7840..7926
                     /gene="uof"
                     /locus_tag="OO566_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6604572.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7919..8365
                     /gene="fur"
                     /locus_tag="OO566_001209"
     CDS             7919..8365
                     /gene="fur"
                     /locus_tag="OO566_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6604573.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8449..8775)
                     /locus_tag="OO566_001210"
     CDS             complement(8449..8775)
                     /locus_tag="OO566_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604574.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8812..10218)
                     /gene="chiP"
                     /locus_tag="OO566_001211"
     CDS             complement(8812..10218)
                     /gene="chiP"
                     /locus_tag="OO566_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6604575.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLFHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10797..12461)
                     /gene="glnS"
                     /locus_tag="OO566_001212"
     CDS             complement(10797..12461)
                     /gene="glnS"
                     /locus_tag="OO566_001212"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6604576.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12664..14610)
                     /gene="nagE"
                     /locus_tag="OO566_001213"
     CDS             complement(12664..14610)
                     /gene="nagE"
                     /locus_tag="OO566_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6604577.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14943..15743
                     /gene="nagB"
                     /locus_tag="OO566_001214"
     CDS             14943..15743
                     /gene="nagB"
                     /locus_tag="OO566_001214"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6604578.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15803..16951
                     /gene="nagA"
                     /locus_tag="OO566_001215"
     CDS             15803..16951
                     /gene="nagA"
                     /locus_tag="OO566_001215"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6604579.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            16960..18180
                     /gene="nagC"
                     /locus_tag="OO566_001216"
                     /gene_synonym="nagR"
     CDS             16960..18180
                     /gene="nagC"
                     /locus_tag="OO566_001216"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6604580.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18228..18970
                     /gene="nagD"
                     /locus_tag="OO566_001217"
                     /pseudo
     CDS             18228..18970
                     /gene="nagD"
                     /locus_tag="OO566_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19367..21031
                     /gene="asnB"
                     /locus_tag="OO566_001218"
     CDS             19367..21031
                     /gene="asnB"
                     /locus_tag="OO566_001218"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6604581.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21411..21487
                     /locus_tag="OO566_001219"
     tRNA            21411..21487
                     /locus_tag="OO566_001219"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21445..21447,aa:Met,seq:cat)
     gene            21496..21580
                     /locus_tag="OO566_001220"
     tRNA            21496..21580
                     /locus_tag="OO566_001220"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21530..21532,aa:Leu,seq:tag)
     gene            21604..21678
                     /locus_tag="OO566_001221"
     tRNA            21604..21678
                     /locus_tag="OO566_001221"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21636..21638,aa:Gln,seq:ttg)
     gene            21713..21787
                     /locus_tag="OO566_001222"
     tRNA            21713..21787
                     /locus_tag="OO566_001222"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21745..21747,aa:Gln,seq:ttg)
     gene            21803..21879
                     /locus_tag="OO566_001223"
     tRNA            21803..21879
                     /locus_tag="OO566_001223"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21837..21839,aa:Met,seq:cat)
     gene            21928..22002
                     /locus_tag="OO566_001224"
     tRNA            21928..22002
                     /locus_tag="OO566_001224"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21960..21962,aa:Gln,seq:ctg)
     gene            22040..22114
                     /locus_tag="OO566_001225"
     tRNA            22040..22114
                     /locus_tag="OO566_001225"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22072..22074,aa:Gln,seq:ctg)
     gene            complement(22268..23443)
                     /gene="ubiF"
                     /locus_tag="OO566_001226"
     CDS             complement(22268..23443)
                     /gene="ubiF"
                     /locus_tag="OO566_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6604582.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23589..25013
                     /gene="miaB"
                     /locus_tag="OO566_001227"
     CDS             23589..25013
                     /gene="miaB"
                     /locus_tag="OO566_001227"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6604583.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25166..26206
                     /locus_tag="OO566_001228"
     CDS             25166..26206
                     /locus_tag="OO566_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6604584.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAREEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26203..26670
                     /gene="ybeY"
                     /locus_tag="OO566_001229"
     CDS             26203..26670
                     /gene="ybeY"
                     /locus_tag="OO566_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6604585.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26760..27638
                     /gene="corC"
                     /locus_tag="OO566_001230"
     CDS             26760..27638
                     /gene="corC"
                     /locus_tag="OO566_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6604586.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27663..29201
                     /gene="lnt"
                     /locus_tag="OO566_001231"
     CDS             27663..29201
                     /gene="lnt"
                     /locus_tag="OO566_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6604587.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            29598..30505
                     /gene="gltI"
                     /locus_tag="OO566_001232"
                     /pseudo
     CDS             29598..30505
                     /gene="gltI"
                     /locus_tag="OO566_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            30675..31415
                     /gene="gltJ"
                     /locus_tag="OO566_001233"
                     /pseudo
     CDS             30675..31415
                     /gene="gltJ"
                     /locus_tag="OO566_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015698156.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
     gene            31415..32089
                     /gene="gltK"
                     /locus_tag="OO566_001234"
     CDS             31415..32089
                     /gene="gltK"
                     /locus_tag="OO566_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6604588.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32089..32814
                     /gene="gltL"
                     /locus_tag="OO566_001235"
     CDS             32089..32814
                     /gene="gltL"
                     /locus_tag="OO566_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6604589.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            32932..33867
                     /gene="rihA"
                     /locus_tag="OO566_001236"
     CDS             32932..33867
                     /gene="rihA"
                     /locus_tag="OO566_001236"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6604590.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            33951..35621
                     /gene="hscC"
                     /locus_tag="OO566_001237"
     CDS             33951..35621
                     /gene="hscC"
                     /locus_tag="OO566_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR6604591.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(35681..37132)
                     /gene="djlC"
                     /locus_tag="OO566_001238"
     CDS             complement(35681..37132)
                     /gene="djlC"
                     /locus_tag="OO566_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6604592.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37129..>37707)
                     /locus_tag="OO566_001239"
     CDS             complement(37129..>37707)
                     /locus_tag="OO566_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6604593.1"
                     /translation="SWGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKAL
                     IAGGSERGLIYTQFVAQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQS
                     RVYARAHHYYHSWMHYFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEEL
                     AAGSDRFYATLPWQPLTVQPECPVTLKDVSDL"
BASE COUNT         9190 a         9676 c         9572 g         9269 t
ORIGIN      
        1 cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg cgaatcacgg
       61 aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac gcaactgcgc
      121 ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc ttttgactgc
      181 ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca gttcgtggag
      241 tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc aatgggcgat
      301 ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa agtgtttaat
      361 gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt ggttttaaac
      421 gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa atctaaccac
      481 cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc acgtaacccg
      541 tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct gcgtgagctg
      601 atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct cgctgtgatt
      661 cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga taaaattggt
      721 catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca atttatcccg
      781 atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc gggtattcta
      841 gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca aatccataaa
      901 aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat gaacaacgcc
      961 tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact ggatatcaac
     1021 gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt ggtaaatggc
     1081 attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt cgtacctgaa
     1141 ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt tgatctgcgc
     1201 ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc agagaatcaa
     1261 tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc acgtaacggc
     1321 gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg cgaaaatggc
     1381 gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc ggcagaagat
     1441 atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact gcttgagtcc
     1501 gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga gcgctacgcc
     1561 ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg ccacaacgtg
     1621 aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag catgaatccg
     1681 caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct gccggatgcc
     1741 gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct gtgtgttgtt
     1801 ccgggtgaaa tctggggttg tgctgttttg cgttacttca gcgctctgga agaagggatc
     1861 aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga acatgatggt
     1921 cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac cctgttgaaa
     1981 ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag ttaaccatac
     2041 tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag cttgccgaag
     2101 ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg gcactggcat
     2161 gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc ggttatgccg
     2221 agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc gtctcactgc
     2281 tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa ctgctcggcg
     2341 cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg atttgtaccg
     2401 tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc gtgtgggggg
     2461 tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc ccgacgcttt
     2521 acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt tcttccatca
     2581 ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact gatgtagtgg
     2641 aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt gcggcggtga
     2701 tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag ctggcaaatt
     2761 caacggcacc atttggcctg gccttcgcac agatgttcac accagaagtg ggtaaagtca
     2821 ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag ttcaccattg
     2881 cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc tcccgtgtaa
     2941 ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag agcgggttgc
     3001 cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt aacctggccg
     3061 tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt attcagaagg
     3121 tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt gttggcgcga
     3181 tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac ggttccatcg
     3241 tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa ctgaaaaata
     3301 aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt tttttattaa
     3361 atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt tctcaatctg
     3421 cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt cttccgtgcc
     3481 tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc caatagaagc
     3541 accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca gggtgctggc
     3601 gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag tcgcagctgc
     3661 ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt agtgttcctg
     3721 cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa tgatgccgtc
     3781 cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg cgctctcttc
     3841 gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga aacctaccgg
     3901 tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg cagaagaaac
     3961 cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga acaggtagtt
     4021 gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga cgataccgtg
     4081 acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca gtgccagcag
     4141 acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt tatcgaggtg
     4201 cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga ggttgtaata
     4261 ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa cgcccagcgt
     4321 caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt ccacgaacgg
     4381 ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga gggagatacg
     4441 cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca ccactttggt
     4501 gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac catcttccgg
     4561 cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt ttttattgtg
     4621 aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt cctgcacaat
     4681 gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag gctcggacag
     4741 ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg cacgttcttc
     4801 agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc gcgctgcact
     4861 gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg cttctggttt
     4921 cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct gttgtgcagg
     4981 ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg ttttagatag
     5041 ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg atgtgttcga
     5101 tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc gtgcctggca
     5161 catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct gccgcaaagt
     5221 acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg gcgtcaagag
     5281 aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc tctgcgtatt
     5341 cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt ttgaccggct
     5401 tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga gcagcaggct
     5461 gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc tcgccgatat
     5521 gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc gttttcatct
     5581 taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa taaatcccgg
     5641 cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta tttgttgcag
     5701 ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa aagaactttg
     5761 ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat tctcccatcg
     5821 ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct cgcgatctgg
     5881 taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca ccaagagagc
     5941 cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat gcactgcaga
     6001 tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg gcacttactg
     6061 cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg gtcgactatc
     6121 acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa tcggacgcac
     6181 aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa ggggtgattc
     6241 agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg gtattgtggg
     6301 atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac cctgccctgt
     6361 ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat ttactggcac
     6421 aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc catgctgaaa
     6481 aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc aactgattaa
     6541 aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgagtcat cgcgctgatg
     6601 tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc cgaagtcacc
     6661 aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc gcacgagcgt
     6721 cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg tattaataaa
     6781 cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact gaagctgaac
     6841 gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag ccgtagcgaa
     6901 ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg catcgtttaa
     6961 atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc cgcaagactg
     7021 cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt tcaataagtt
     7081 tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg acaccggtaa
     7141 taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg ccgatgtcca
     7201 tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc tgctgggcat
     7261 cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc cgactctcga
     7321 agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc aggaagatta
     7381 cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac cgcgcggtgc
     7441 aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa aaggtctggc
     7501 agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg aactgaccgc
     7561 tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg acgaaattct
     7621 taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag ataatgccaa
     7681 tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat gtcccagtgt
     7741 tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac aatatttgcc
     7801 agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat tatctcaaga
     7861 gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca gattccgcat
     7921 gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc ctcgtttaaa
     7981 aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag atttatacaa
     8041 acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg tactgaacca
     8101 gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat ccgtatttga
     8161 gctgacacag caacatcacc acgatcacct gatttgcctc gactgcggca aggttatcga
     8221 atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac atggcattcg
     8281 cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt gccgcgaaga
     8341 tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac ctgcgggttg
     8401 gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt atttgttatt
     8461 actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat catttccggt
     8521 ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct ggtcggcaaa
     8581 ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctggcagg cttcaatttt
     8641 ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca gacggctgtc
     8701 ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca tggcgatgag
     8761 aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc atcagaagat
     8821 ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga tgttaccgta
     8881 accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt gcagtttgaa
     8941 catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc tgtaggcaga
     9001 ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc aggtcgcagg
     9061 tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa gattccagtt
     9121 tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt tgaagtagga
     9181 acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg gggtcatacg
     9241 ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga ggcgcaaatc
     9301 cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg tgccgtcata
     9361 aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact ggtagctggt
     9421 ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat attgatcgat
     9481 atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga agtcgtattt
     9541 cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat aaaactcatc
     9601 catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga aactcaaggc
     9661 accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag tacccggcat
     9721 aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac ctgccctcgc
     9781 ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac cgcttttgtc
     9841 gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt cgttcgggtg
     9901 ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat caaggccgaa
     9961 catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg agtgaaaaag
    10021 gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac gctgccagta
    10081 atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag cctccggcat
    10141 tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg tactacgttt
    10201 gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc accaaaggtg
    10261 aacagcgaaa aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg ctgactacag
    10321 gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag aatgtaagaa
    10381 catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt gcggttgaaa
    10441 ggcttgcaga agaataaaaa cgtgaaattc cagcaagatg aaagcggtta cattttattt
    10501 aattatgttt tgtgatttta aatttatttt atggataaat tgcgaagcgg aattaattcg
    10561 cgaagaaaag cagaaaaaac gccgcagagc gcggcgtttg ggattgtttg ctatttcttg
    10621 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca cgcaaattca atatattgca
    10681 gagatcatgt aggcctaata agcgaagcgc atcaggcaat ttagcgtttg aaatttattg
    10741 tctatttatt gccggatgcg acgtaaatgc ctcacccggc ttggtagaac ttaaaattac
    10801 tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac cggtttttcg
    10861 gccgtggaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg gaatgcttta
    10921 cccgctaccg catctttcag cgacggttca gcaaagccct gtttgatcac cagcgattcc
    10981 gggttaatca ccgacaggaa atcatccgca gcacctgggt taggcacgct gaacagacga
    11041 tcatacaaac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg aataacacct
    11101 ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata agtacagaag
    11161 atggtggtga tattaccttc ggcatctttc tccacgcgtt cagccttaat gacgtaagca
    11221 ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt agcttcttcg
    11281 cggaaatcgg cgcgatcgat ccaaatttca ccgctaaacg gcacctgacg gctccccatt
    11341 tccggtttgt tcggatggtt cggcatggtg accatttcgc cttcgccctg atagttttcg
    11401 ataaccagtt tcaccggatc gataactgcc attgcgcgcg gcgcattttc gttgagatct
    11461 tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt ggtcacgccg
    11521 atgcgtttgc agaactcacg aatagaagcc gcagtgtaac cacgacgacg cagaccggaa
    11581 atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac cagcaggttc
    11641 agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc gtactggcgc
    11701 ggatgaacag gaatggtgat gttatccagt acccagtcgt acagacgacg gttgtcctgg
    11761 aactcaagcg tacacagaga gtgcgtaata ccttccagcg catcgctgat gcaatgggtg
    11821 aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc agcaaattta
    11881 atgcggtaca gcaccggatc gcgcattacg ataaatggcg acgccatgtc gattttcgca
    11941 cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa cagcgccaag
    12001 ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggctgcgt cagggtaccg
    12061 cggtattcgc ggatctgttc cggtgtcagt tcatcaacgt acgccaggcc tttattgatc
    12121 agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg gacgttacca
    12181 gaccagtgaa aacctaacca ctctacgtcg tttttgatcg aatcgacata ctcgatatct
    12241 tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata gtcctgggcg
    12301 atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc attcggctcc
    12361 ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc
    12421 tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc ctcaaagcgt
    12481 aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact gttagttttt
    12541 taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa gcggcagaga
    12601 agatctccac cgcttgtcat tgttggatgc gacgctgccg tgtcgcatca ggcataaagc
    12661 agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc cctgagcttt
    12721 aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa tcatcgagcg
    12781 ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg cgcttacctg
    12841 cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc
    12901 catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt ggttggtgtt
    12961 gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg tcggtttcac
    13021 cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct gatccagtgc
    13081 cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt ttggtacagc
    13141 ctgcggtttt gctacaggcg cggcagttgc cggagttgct tcagctgacg cagcggctac
    13201 cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt tcgcgccaac
    13261 aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca gacgtttaca
    13321 catcgtatcg ttgacgcggg cagagtcagc cacggtcagg cgcagacggg taatacaggc
    13381 atcaatcgct ttcaggttgt cagtaccgcc aaccgcagca atatagttgg ttgccagttg
    13441 agtcagacct tcttcagtat tgctgttggc ttcttcagta acgatctcgt cttctttatc
    13501 ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca ccacgaagta
    13561 gatagcgaag aagataacgc ccatcaccag cagcatccag acgttctggc tggcggccgg
    13621 caggttatac atcaacgcgt agtcgatagc acccgcagag aaagagaagc cggcatggat
    13681 acccagcagc gttgccacaa acaggctgat accggtcagc agtgcgtgca ggaggtacag
    13741 cagcggcgca aggaacatga acaggaattc cagcggctca gtcacaccgg tcaggaacgc
    13801 agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt tcggtgctgc
    13861 gaagtacatc gccagcgccg cacccggcag accgaacatc atgatcggga agaagccgga
    13921 catgaacatc cccgcggtgc cgtcaccagc gtagaaacgg ttgatgtcgc cgtggaaaac
    13981 cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt tcagcacctg
    14041 atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg aacccagcgc
    14101 gcccgcagaa acgatccact cgccgcctgc atggatagcg tgctgtaccg gcggccagac
    14161 gtaaccaaaa atggccgcca gcaccagaca gaagaagccg gtggcgatcg gcacaaagcg
    14221 tttgccgccg aagaagctca ggaagtccgg cagtttaata tcggaccaac ggttataggc
    14281 tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa tttctgggtt
    14341 gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc
    14401 acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta atgcgaggtt
    14461 atcaaaaatt gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc
    14521 gaatcgcagc aacagcgccg ccaccggcag caccgcgata gggagctgta acgccctacc
    14581 gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac cctatttggc
    14641 tcgtttaaag ccgtattttt attttgctgc aaattttact gccgatgttc tgtaatcaga
    14701 ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa attaaacgcg
    14761 tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta ctggctaaac
    14821 cagaaaactt attttatcat tcaaaaaatc aagtcggatt gacgcctgtc tgcgcaaatc
    14881 caggttacgc ttaaagatgt ctaatccgcc aacggcttac attttactta ttgaggtgaa
    14941 taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct gctcgccata
    15001 tcgtcaatcg tatcaatgcg ttcaaaccaa ctgccgatcg tccgtttgta ctgggcctgc
    15061 cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat aaagcaggcc
    15121 aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt ctgccgaaag
    15181 agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac gttgatattc
    15241 cagcagaaaa catcaacctt ctcaacggca atgccccgga tatcgatgcc gagtgccgcc
    15301 agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc ggtgtaggta
    15361 acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt actcgtatca
    15421 aaaccctgac tcatgacact cgcgtcgcga actctcgttt ctttgataac gatgtcaatc
    15481 aggtgccaaa atatgccctg actgtcggtg ttggtacact gctggatgcc gaagaagtga
    15541 tgattctggt gctgggtagc cagaaagcac tggcgctgca ggccgccgtt gaaggttgcg
    15601 tgaaccatat gtggaccatc agctgtctgc aactgcatcc gaaagcgatc atggtgtgcg
    15661 atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat gaattagaag
    15721 cagaaaatat caaaggtctg taattgttat ccctgccctg gttccttgct cagggcaata
    15781 ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct ttaccggcca
    15841 cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa gcgtctgtcc
    15901 ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca ttctctcccc
    15961 cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg acaccgctga
    16021 agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat caggctgtac
    16081 taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg gcgtgcgcgt
    16141 tatgcgcgag tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc tggaaggtcc
    16201 gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta agcctgatgc
    16261 cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga ccctggcacc
    16321 ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg tggtttctgc
    16381 cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg ggattacctt
    16441 tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg gcctggcggg
    16501 cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc tgcatgttga
    16561 ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc tggttaccga
    16621 cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta aaacaatata
    16681 ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat ccttaaccat
    16741 gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg aagtgctgcg
    16801 tatggcgacg ctctatccgg cgcgtgcgat tgacgttgaa aaacgtctcg gcacgctcgc
    16861 cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca ccaagaccat
    16921 cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg cggacaagct
    16981 cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta tcgcctgatt
    17041 gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct tgcccccgcc
    17101 agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga agttgatcag
    17161 caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg caatttccac
    17221 gcaatcggcg tacggcttgg tcgtcatgac gccaccatca ctctgtttga tctcagcagc
    17281 aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcagac gctggaacat
    17341 gccctgctga atgccattgc tcagtttatt gatagctacc agcgcaagct gcgcgagcta
    17401 atcgccattt cggtgatcct gccagggctt gttgacccgg acagcggcaa aattcattac
    17461 atgccgcata ttcaggtaga aaactggggg ctggtagaag cactgaaaga acgttttaaa
    17521 gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca ctacttcggt
    17581 gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac cggggccggg
    17641 attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga aattggccat
    17701 attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg cctggaaact
    17761 atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca gggctaccag
    17821 agccgtgtgc cactggacga ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc
    17881 gatagcctgg cctcggaagt gattgagtat gtcggtcgtc atctgggtaa aaccatcgcc
    17941 attgctatca acttatttaa tccgcaaaaa attgttattg ctggtgaaat caccgaagcc
    18001 gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaagcgct gaaggcgttt
    18061 cgtactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat cggcgctttt
    18121 gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct ggaaaattaa
    18181 tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg accattaaaa
    18241 atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag
    18301 cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg accaactatc
    18361 cttcgcagac tgggcaagat ctggcgaacc gctttgccac tgccggtgtc gatgtgccgg
    18421 atagcgtgtt ttatacctct gcgatggcga ccgccgattt cctgcgtcgc caggaaggca
    18481 agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa gccggtttca
    18541 ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacacgttcc tacaactggg
    18601 acatgatgca taaagcagcc tatttcgtcg ctaacggtgc acgctttatc gccaccaacc
    18661 cggacacggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga aaatctccgg
    18721 gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcgg cattaaacaa
    18781 aatgcaggcg cattcggaag aaacagtgat tgtcggcgat aacctgcgta ccgatattct
    18841 ggcgggcttc caggccggtc tggagacgat tctggtgctt tctggtgttt catcgctcga
    18901 cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcagtcg ctgaaatcga
    18961 cgttatctga ataccaccca cgtctgcgga cgtggttggc tctgatgaca aacgccaaac
    19021 tgcctgatgc gctacgctta tcaggcctac gcagctcctg caatatattg aattttcatg
    19081 cttttgtagg ccgtataagg cgttcacgcc gcatccggca tgaacaaatc gcactttatc
    19141 agcaatttgg cccccgtaaa atataaccat tcaataaaac ccgctaaaaa ttatcgattc
    19201 atcaataaat acatcctcac accagaccct ttttcaccaa tcgacaatca ccattacgtt
    19261 ttttattttt gcactgccaa atagcttgcg cacagtgctg ctttgcggca tttttttaaa
    19321 caagcaaaca caacaagcaa caaataccag gttaacggag aaggttatgt gttcaatttt
    19381 tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac tcgagctgtc
    19441 acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg ataacgccat
    19501 tctcgctcac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac ctctctacaa
    19561 ccaacaaaaa acccacgtac tggcggtaaa cggtgaaatc tacaaccacc aggcattgcg
    19621 cgctgaatat ggcgatcgtt atcagttcca gacagggtct gactgtgaag tgatcctcgc
    19681 gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt ttgcctttgc
    19741 cctgtacgac agcgaaaaag atgcctacct gattggtcgc gaccatctgg ggatcatccc
    19801 actgtatatg gggtatgacg aacacggtca gctgtatgtg gcctcagaaa tgaaatcgct
    19861 ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt ggagtcagga
    19921 cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag tgaaagataa
    19981 cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa gccatctgat
    20041 gtctgatgtg ccttacggtg tgctactttc tggtggtctg gattcctcaa ttatttccgc
    20101 tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg
    20161 gccgcagtta cactcctttg ctgtaggtct gccgggttca ccggatctta aagcagccca
    20221 ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg tacaggaagg
    20281 tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga ccactattcg
    20341 cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca ttaaaatggt
    20401 gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc ataaagcgcc
    20461 caacgctaaa gaactgcatg aagagacggt gcgtaaactg ctggccctgc atatgtatga
    20521 ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct
    20581 cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa tgtgcggtaa
    20641 cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc ccgcaagcgt
    20701 ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga tcgacaccct
    20761 gaaagaagtg gcggcgcagc aggtttctga tcagcaactg gaaactgccc gcttccgctt
    20821 cccgtacaac acgccgacct caaaagaagc gtatctgtac cgcgagatct ttgaagaact
    20881 gttcccactt ccgagtgccg ctgagtgcgt gccgggcggt ccttccgtcg cgtgttcttc
    20941 agctaaagcg atcgaatggg atgaagcgtt caaaaaaatg gacgatccgt ctggtcgtgc
    21001 ggttggcgtt caccagtcgg cgtataagta agaaatgcgg tactgccccg gagaaatccg
    21061 gggctgtttt agttattgcg ctgcttgttg attctgatat gcagtaagga aatgttccgg
    21121 gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat agattaattt
    21181 cccaccacta ttgttgaatt ccgaatttca tcctgtacga caaaagaatg gctgtagggt
    21241 ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg ttcgcaacct
    21301 aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct
    21361 atacgcataa tgcgccccgc aacgcagata aggtatcgcg aaaaaaagat ggctacgtag
    21421 ctcagttggt tagagcacat cactcataat gatggggtca caggttcgaa tcccgtcgta
    21481 gccaccatct tttttgcggg agtggcgaaa ttggtagacg caccagattt aggttctggc
    21541 gccgcaaggt gtgcgagttc aagtctcgcc tcccgcacca ttcaccagaa agcgttgtac
    21601 ggatggggta tcgccaagcg gtaaggcacc ggtttttgat accggcattc cctggttcga
    21661 atccaggtac cccagccatc ttcttcgagt aagcggttca ccgcccggtt attggggtat
    21721 cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc
    21781 ccagccatcg aagaaataat ctggctacgt agctcagttg gttagagcac atcactcata
    21841 atgatggggt cacaggttcg aatcccgtcg tagccaccaa attctgaatg tgtcgaatac
    21901 gttcggcaaa ttcaaaaacc aatttgttgg ggtatcgcca agcggtaagg caccggattc
    21961 tgattccggc attccgaggt tcgaatcctc gtaccccagc caatttattc aagacgctta
    22021 catcgtaagt gcacccagtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg
    22081 gcattccgag gttcgaatcc tcgtacccca gccacattaa aaaagctcgc ttcggcgagc
    22141 tttttgcttt tctgcgttca ttcaatgtcg aatgcgatgt tgacacgtct tatccttcaa
    22201 tgtcggatgc gacgctgccg cgtcttatcc gacctacggt tggcacgcat ccggcaatgt
    22261 tgtaaggcta caaccctaac gcatatttca gcgcctgacg tttcaacacg ccagcacgct
    22321 ctgccgccat taacccgaga ttacgcacaa aacgcagcgg cggcagatta ttactgaatc
    22381 cggcataaaa cagatccata ccactttgca taatgaagtt atccgccatg cgccgcatct
    22441 ggtaacgctt gaggacagga taactggccc acgcttcacc gtagctgcgg gcattcacca
    22501 gaacatcaat cagggcatcg acatcacgat aaccaagatt caccccctgc cccgccagcg
    22561 gatggatggt atgcgcggca tcgcccacca gcgcaagccc cggctgcaca tactgcaacg
    22621 catgtcggcg cgtcagcgga aacgcaccag cggcaagcgg tgtaacgtaa cccagacgcg
    22681 acgggaaatg cttcgcgatt tccgcctgga gttgcgccat attcatattc tgcaactggc
    22741 gaatacgcgc cggagagtca taccacacca gcgatgccca gttatcaaac aacggcagaa
    22801 acgcacgcgg tccgtccggg gtaaattgct gccaggtgct gtcgccggga tcgttctcac
    22861 actgtacgct aatcaacatg cacgactgcg catactgcca ggcatgaacg ccaattcccg
    22921 ccatctgccg cacctgagaa tttgcgccgt cggcaccaat caccagtttc gcgcggatcg
    22981 tttcaccgcc cttcagttcc agctcctgaa gatcattatc tcgatgtaat gcaatcagcg
    23041 aggttggcac acgtaacgtt actttcggat gcgcttccag cgcctgccac aacgcctgtt
    23101 gcaggactgt gttttccacc atgtagccaa gcaacggcaa ctttaattcg gctgcgtcaa
    23161 ataccacatg tgccgtttcc cactcccagg tttccagtct gcggtaagga tggcaacgca
    23221 tagcctgtac tgcatcccag acccctaccc ctttaagcaa tgataccgaa gccgcgctga
    23281 tcgccgagat ccgcacgtcc ggttggctgt cagcgacaaa cggtgcaggt tctgcgtgct
    23341 cgatcaccgt taccgaaaat ccgtgctgtg ccagccccag cgccagtgcg ccgccgacca
    23401 ttcctccgcc gacaatggca atttccgttg gttgatttgt catggtcaat catcctgtta
    23461 gcaatatgct ttaagtttac aggattttta cccccaaggg ctgataacgc tcatactggt
    23521 cacaacggca gcaaagcatt acactatgcg ccctgcattc ctggctacta tttcgcaaga
    23581 gcaagtcgat gaccaaaaaa ctccatatta aaacctgggg ctgtcagatg aacgagtacg
    23641 attcatcgaa gatggccgat ctgctggatg ccacccacgg ctatcaactg accgacgtgg
    23701 cggaagaagc ggatgtgcta ctgctgaaca cctgctcaat ccgcgagaag gctcaggaaa
    23761 aagtcttcca tcagttgggt cgctggaaac tgttaaaaga gaagaatcca gacctgatta
    23821 tcggcgtcgg tggctgcgtg gcatcgcaag aaggcgagca cattcgccag cgcgcccact
    23881 atgtcgatat tatttttggg ccgcaaacgc tgcaccgtct gccggagatg atcaactccg
    23941 tgcgcggcga ccgcagcccg gttgtagata tcagcttccc ggaaatcgag aagtttgacc
    24001 gtctgccgga accgcgcgcc gaagggccga ccgcgtttgt ctccatcatg gaaggctgca
    24061 ataaatattg cacctactgc gtggtgcctt acacccgtgg tgaagaggta agccgtccgt
    24121 ctgacgatat tctgtttgag attgcccagc ttgcggctca gggcgtgcgt gaagtcaacc
    24181 tgctcggtca gaatgtgaat gcctggcgtg gtgagaacta cgacggcacc accggatcgt
    24241 ttgccgatct gttgcgtctg gttgctgcga tcgacgggat cgatcgtatt cgctttacca
    24301 ccagccatcc gatcgaattc accgacgata tcatcgaagt gtaccgcgac acgcctgagc
    24361 tggtgagctt cctgcatctg ccggtacaga gcggttccga tcgcattctg aacctgatgg
    24421 gacgtaccca tacggcgctg gagtacaaag cgatcatccg taaactgcgt gcggcgcgtc
    24481 cggatattca gatcagttct gacttcattg ttggcttccc tggcgaaacc accgaagact
    24541 tcgagaaaac gatgaagctg attgccgacg tcaatttcga catgagctac agctttatct
    24601 tctctgcacg tccaggaacg cctgccgccg atatggttga tgatgttccg gaagaagaga
    24661 agaagcagcg tctgtatatt ctgcaagaac gtatcaatca gcaagcgatg gcatggagcc
    24721 gtcggatgct cggcaccacc cagcgtattc tggtagaagg gacttcgcgt aagagcatca
    24781 tggagctttc cgggcgtacc gaaaataacc gcgtggtcaa cttcgagggc acaccggata
    24841 tgatcggtaa attcgtcgat gtagaaatta ccgacgtcta cccgaactct ctgcgcggta
    24901 aagtggtgcg aactgaagat gaaatgggtc tgcgcatggc agaaacaccg gagtcggtga
    24961 ttgcccgcac ccgcaaagaa aacgaccttg gcgtgggtta ttatcagccg taattctcag
    25021 gcctgccgtt ccggtgggcc ttgttttttc tctctttatc cccatcttta tcgcaatgct
    25081 tgcgcctttt tcctgtggcg tgaataattt ccttataggc cggtgaatgt tcagcttccg
    25141 gcggcataca gataagagga acggtttgaa catagacact cgcgaaatca ccctggagcc
    25201 agcagacaat gcgcgtctgt tgagcctgtg cggcccgttt gatgacaaca tcaagcagct
    25261 cgaacgccgt ctcggcatcg agatcaatcg ccgcgataac cactttaaac tgaccggccg
    25321 tccgatttgc gtcaccgctg cggcagacat tctgcgtagc ctgtatgtcg atactgcccc
    25381 gatgcgcggt cagattcagg atatcgaacc ggaacagatc caccttgcga ttaaagaagc
    25441 gcgggtactg gagcaaagcg cggagagcgt gccggagtac ggcaaagcgg tcaatatcaa
    25501 aactaaacgc ggcgtaatta agccgcgcac gccaaaccag gcgcagtaca tcgccaatat
    25561 tctcgaccat gacattacct tcggcgttgg cccggcgggt acgggtaaaa cctacctggc
    25621 agtggctgcg gcagttgatg ccctggagcg tcaggaaatt cgccgtattc tgctgactcg
    25681 tccggcggtc gaagccggtg agaaactggg cttcctgcct ggcgatttaa gccagaaagt
    25741 agacccgtat ctgcgcccac tgtacgacgc gctgtttgaa atgctgggct ttgagaaagt
    25801 cgagaaactg attgagcgca acgttattga agtcgcgccg ctggcctata tgcgtggtcg
    25861 tacgctgaac gacgcgttta tcattctcga tgagagccag aacactacca tcgaacagat
    25921 gaagatgttc ctgacccgta tcggttttaa ctcaaaagcg gttatcaccg gcgacgtcac
    25981 acagatcgac ctgccgcgta atactaaatc aggcttacgt catgccattg aagtgctggc
    26041 cgatgtcgaa gagatcagct ttaacttctt ccacagcgaa gacgtggttc gtcacccggt
    26101 ggtggcgcgt atcgttaacg cttatgaagc cagggaagaa gcagaacaaa aacgtaaagc
    26161 ggcgctggca gcagaacgca agcgcgaaga acaggaacaa aaatgagtca ggtgatcctc
    26221 gatttacaac tggcatgtga agataattcc gggttaccgg aagagagcca gtttcaaaca
    26281 tggctgaatg cggtgatccc gcagtttcag gaagagtcgg aagtgactat tcgcgtggtc
    26341 gataccgccg aaagccacag tctgaatctg acctaccgcg gtaaggataa gccgaccaac
    26401 gtgctctcct tcccgtttga agtgccgcct ggcatggaga tgtcgctact gggcgatctg
    26461 gttatctgcc gtcaggtggt tgagaaggaa gctcaggagc aaggcaaacc actggaggcg
    26521 cactgggcgc atatggtggt gcacggcagt ctgcatttgt taggttacga tcacattgaa
    26581 gatgacgaag cagaagaaat ggaagccctc gaaacagaga ttatgcttgc tctgggctat
    26641 gaggatccgt acattgccga gaaagaataa tttgccaggc atttgactgg cagacgtccc
    26701 cgccgttggt gattaacgac ggcgttttga ttaactaaca tgagaaccca tacgacgcca
    26761 tgagcgacga caattcacac agtagtgaca cgataagcaa caagaaggga tttttctccc
    26821 tgttactcag ccaacttttc cacggtgaac cgaaaaaccg tgacgaactg ctggcgctga
    26881 tccgtgattc cgggcagaac gaccttatcg acgaagatac gcgcgatatg ctcgaagggg
    26941 tgatggacat cgcagaccaa cgcgttcgcg acatcatgat cccccgctcc cagatgatta
    27001 ccctgaaacg caaccagacg ctggacgaat gccttgatgt catcatcgag tccgcccact
    27061 cacgtttccc ggtgattagc gaagacaaag atcacattga agggattctg atggcgaaag
    27121 acttgctgcc gtttatgcgc agcgatgctg aagccttcgg catggacaaa gtgttacgtc
    27181 aggcggttgt cgttcctgaa agtaagcgcg tagaccggat gctgaaagag tttcgctctc
    27241 agcgttacca catggcgatc gttattgacg aattcggtgg ggtttccggt ctggtgacca
    27301 ttgaagacat cctggaactg attgttggtg agattgaaga cgagtatgac gaagaagatg
    27361 atatcgactt ccgtcagctg agtcgtcata cctggaccgt gcgcgcactg gcttccattg
    27421 aagacttcaa cgaagcgttc ggcacccact ttagcgatga agaagtcgac actatcggtg
    27481 gtctggtgat gcaggcgttt gggcatcttc cggcgcgtgg cgaaactatc gacatcgacg
    27541 gttaccagtt caaagtggcg atggccgata gtcggcgtat tattcaggtt catgtcaaaa
    27601 tcccggatga ctcaccccag ccaaagctgg atgaataaaa ccgaaactgg atagataact
    27661 acatggcttt tgcctcatta attgaacgcc agcgcattcg cctgctgctg gcgttattat
    27721 tcggtgcctg cggaacgctg gccttctctc cttacgacgt ctggcctgcg gcgattattt
    27781 cgctgatggg gcttcaggcg ttgaccttta accgtcgccc actccagtct gccgctattg
    27841 gcttttgctg gggatttggc ctctttggca gcggtattaa ctgggtctat gtcagcatcg
    27901 cgacctttgg cgggatgcct ggcccggtta acatcttcct agtggtgctg ctggcggcgt
    27961 atttgtcgct gtataccgga ctgtttgctg gcgtgctgtc gcgtctgtgg ccgaaaacca
    28021 cctggctgcg cgtggcgatt gccgcccctg ctctctggca agtgaccgag tttctgcgcg
    28081 gttgggtgct gactggcttc ccgtggttac agttcggcta tagccagatt gatggcccgt
    28141 taaaagggct ggcaccgcta atgggcgtgg aagccattaa cttcctgctg atgatggtta
    28201 gtggcctgct ggcactggcg ttggtcaaac gcaactggcg tccgctggtg gtggccgtcg
    28261 tgctgtttgc cctacccttc ccgctgcgtt acatccaatg gtttaccccg caaccggaga
    28321 aaaccattca ggtttcgatg gttcagggcg atattccgca atcgctgaaa tgggacgaag
    28381 gccagcttct taatacgcta aagatttact acaacgcaac ggcaccgctg atgggcaaat
    28441 catcgttgat tatctggccg gagtcggcga taaccgatct ggaaattaat cagcaaccgt
    28501 tcctcaaagc actggacggt gagttgcgtg ataaaggtag ctcgctggta accgggattg
    28561 tcgacgcgcg tctcaataag cagaaccgct acgataccta caacaccatc atcacgctgg
    28621 gtaaaggtgc accgtacagc tacgaatcag ccgatcgcta taacaaaaac catctggtgc
    28681 cgtttggcga gtttgtcccg ctggagtcga ttctgcgtcc gttagcaccg ttctttgatc
    28741 tgccgatgtc gtcgttcagc cgtgggccat atatccagcc gccgctgtcg gcaaatggta
    28801 ttgagcttac tgcggctatt tgctacgaga tcattctcgg cgagcaagtg cgcgataact
    28861 tccgcccgga taccgactac ctgctgacta tctccaacga tgcgtggttt ggtaaatcta
    28921 ttggtccatg gcaacacttc cagatggcgc gaatgcgtgc gctggagctg gcgcgcccac
    28981 tgttgcgcag caccaacaac ggcattacgg cggtgattgg cccgcagggt gagattcagg
    29041 cgatgatccc gcagttcacc cgcgaggtgt taaccactaa cgtgacgccg accaccggac
    29101 tcacaccata cgcacgtacc ggcaactggc cgctgtgggt gctgacggca ctgtttggtt
    29161 ttgctgctgt gttgatgagt ctgcgtcagc gacgtaaata atcccttctt atcgtgccgg
    29221 aatacatccc atatttcggc acgctcaccg gatgcggcat cagtgcatta tccggataac
    29281 aatattctcc tatccaactt tggcacatct attgctttgt tatacaaggc aaagcttgaa
    29341 ccagcatcag tacaacccgg ttgcaccaca gcaatccacc ggtagcacca aaagagtgca
    29401 ttttaagaat tttgcttcag aatggtgcgg tgcacctcgc aaaaacaaac aataaaacat
    29461 caatatcttt acatttagcc aaactaaatg ttacctaaca ggcacaacac tgcacaataa
    29521 agttgcagac gataacaaca caaacactca caacgggtat ccatgcgttc ttaacgcaga
    29581 agataaagga gttggatatg caattacgta aacctgccac agcaatcctc gccctggcgc
    29641 tttccgcagg actggcacag gcagatgacg ccgccccggc agcaggcagt acgctggaca
    29701 aaatcgccaa aaacggtgtg attgtcgtcg gtcaccgtga atcttcagta cctttctctt
    29761 attacgacaa tcagcaaaaa gtggtgggtt actcgcagga ttactccaac gccattgttg
    29821 aagcagtgaa aaagaaactc aacaaaccgg acttgcaggt aaaactgatt ccgattacct
    29881 cacaaaccgt attccactgc tgcaaaacgg cactttcgat tttgaatgtg gttctaccac
    29941 caacaacgtc gaacgccaaa aataggcggc tttctctgac actattttcg tggtcggtac
    30001 gcgcctgttg accaaaaagg gcggcgatat caaagatttt gccgacctga aaggcaaagc
    30061 cgtagtcgtc acttccggca ctacctctga agttttgctc aacaaactga atgaagagca
    30121 aaaaatgaat atgcgcatca tcagcgccaa agatcacggt gactctttcc gcaccctgga
    30181 aagcggtcgt gccgttgcct ttatgatgga tgacgctctg ctggccggtg aacgtgcgaa
    30241 agcgaagaaa ccagacaact gggatatcgt cggcaagccg cagtctcagg aagcctacgg
    30301 ttgcatgttg cgtaaagacg atccgcagtt caaaaagctg atggatgaca ctatcgctca
    30361 ggtgcagacc tccggtgaag cggaaaaatg gtttgataaa tggttcaaaa atccaattcc
    30421 gccgaaaaac ctgaacatga atttcgaact gtcagacgaa atgaaagcac tgttcaaaga
    30481 accgaatgac aaggcactga actaattaca agaaccagag gcggaaattc cagccctctc
    30541 gattgttacg tagcacggac agactatacg cctgatggtc gttccccatc gggcctgaaa
    30601 accgcaatac gctgggtaac aatcttcgag ggtagcagtt aacgctgcta ccctcttttt
    30661 tctggagtag atttatgtct atagactgga actggggtat ttttttacaa caagccccgt
    30721 tcggcaatac cacctatctt ggttgggtct ggagtggttt tcaggtgacg atcgctttgt
    30781 cgatctgcgc ctgaattatc gctttcctcg tcggttcatt tttcggcatt ttacgtaccg
    30841 ttccaaaccg ttttctctcc ggtctgggta cgttgtatgt cgaactgttc cgcaacgtgc
    30901 cgttgattgt gcaattcttt acctggtatc tggtgatccc ggagctgctg ccggagaaaa
    30961 tcggcatgtg gtttaaggcc gagctggatc ccaatattca gtttttcctt tcatccatgc
    31021 tctgcctggg gctgtttacc gcctcccgtg tttgcgaaca ggtacgcgcc gctattcagt
    31081 cgctaccgcg tgggcagaag aatgccgcgc tggcgatggg cctgacgcta ccgcagactt
    31141 atcgatacgt actgctgcct aatgcttatc gcgttatcgt cccgccgatg acttccgaga
    31201 tgatgaacct ggtgaaaaac tccgccatcg cctcgaccat cggtctggtg gatatggcgg
    31261 cgcaggcggg taaattgctc gactactcgg ctcacgcctg ggagtcgttt accgccatca
    31321 cgcttacgta tgtgttgatt aacgctttta tcatgctggt gatgacgctg gttgaacgta
    31381 aagttcgcct gcccggcaac atggggggca aataatgtac gagtttgact ggagttccat
    31441 tgttccttcc ctgccatatc tgctcgacgg gctggtgatc accctgaaaa tcaccgtcac
    31501 ggcagtcgtg attggtattt tgtggggtac catgctggcg gtgatgcgct tgtccagctt
    31561 cgcgcctgtt tcgtggtttg ccaaagccta cgttaacgta ttccgctcga ttcctttagt
    31621 catggttttg ctgtggtttt acttgattgt gccgggattt ctgcaaaatg tgctgggatt
    31681 atcgccaaaa aatgatattc gtttaatttc ggcgatggtg gcgttttcga tgtttgaagc
    31741 ggcctactat tcagagatta tccgtgccgg tattcaaagt atttcccgtg gtcagtcgag
    31801 cgccgcgctg gcgttgggga tgactcactg gcagtcgatg aaactgatta ttctgccgca
    31861 ggctttccgc gcgatggtgc cgctgctgct cactcagggc atcgtactgt tccaggatac
    31921 ctcgctggtg tatgtgttat ctctggccga tttcttccgt accgcctcaa ccattggtga
    31981 gcgtgatggt acgcaggtcg agatgatcct gtttgccgga tttgtttatt tcgttattag
    32041 ccttagcgcg tcgttgttgg tcagctactt gaaaagaagg acagcataat gattaccctg
    32101 aaaaatgttt caaaatggta tggtcacttt caggtgctga ccgactgctc aaccgaagtg
    32161 aaaaaaggcg aagtggtggt ggtttgcggc ccgtctggtt ccggcaaatc aacgctgatt
    32221 aaaaccgtca acggcctcga accagtgcag caaggtgaaa tcaccgtcga tggtatcgtg
    32281 gttaacgaca agaaaaccga tctggcaaag ctgcgctccc gcgtcgggat ggtattccag
    32341 catttcgagc tgttccctca tctgtcgatt atcgaaaacc tgaccctggc gcaggtgaaa
    32401 gtgcttaaac gcgataaagc gcccgctcgt gaaaaagcat tgaaattgct ggagcgcgtg
    32461 ggactttccg cacatgccaa taaatttccg gcgcagcttt ccggcggtca gcagcagcgt
    32521 gtggcaatcg ctcgcgcatt gtgtatggct cctattgcga tgctgttcga cgaaccgaca
    32581 tcggcgctgg atccggagat gatcaacgaa gtgctggacg tgatggtgga actggcgaac
    32641 gaaggaatga ccatgatggt ggtgacccac gaaatgggct ttgcccgcaa agtggcgaat
    32701 cgggtgatct ttatggacga gggtaaaatt gtcgaagact cgccgaaaga cgctttcttc
    32761 gatgatccga aatcggaccg cgcaaaagac ttcctcgcga aaatcctgca ttaatcactc
    32821 tggcgcgcat acccaattgc gcgccatctc acgctttatc gtttcgcccc tcgtatctgt
    32881 tacatcctgg cgttaccctt gtcgcaaaga agcacacaac aaggagcaac aatggcactg
    32941 ccaattctgt tagattgcga cccaggtcat gatgacgcta tcgcaatagt tctcgccctc
    33001 gcctcaccag agcttgatgt caaagcaatt acgtcttccg ccggaaacca gacaccagaa
    33061 aaaaccttac gcaatgttct gcgtatgctg accttgctta atcgcaccga tattccggtg
    33121 gcaggcggcg cggtaaaacc gttaatgcgt gagttgatta tcgcggacaa tgtgcacggc
    33181 gaaagcggtc tcgacggccc ggcattaccg gaaccgacgt tcgcaccgca aaactgtacg
    33241 gcggtggagc tgatggcgaa aacgctgcgc gaaagtgcgg aacctgtcac gattgtttct
    33301 accggaccgc aaactaacgt tgccttgctg ctcaatagcc atccggaact gcatagcaaa
    33361 attgcccgta tcgtgattat gggcggcgca atggggcttg gtaactggac gcctgcggct
    33421 gaatttaata tttacgttga cccggaagcg gcagaaattg tcttccagtc agggatcccg
    33481 gtggtgatgg ccggtctgga tgttactcat aaagcgcaaa tccacgttga agacaccgag
    33541 cgtttccgcg cgattggcaa ccctgtttca accattgttg ccgaactgct ggatttcttc
    33601 ctcgaatatc ataaagacga aaaatggggc tttgtcggcg caccgctgca tgacccatgc
    33661 accatcgcct ggctgttgaa acccgagttg tttaccactg ttgagcgctg ggttggcgtg
    33721 gaaacacagg ggaaatatac cctgggtatg acggttgttg attattatta tctgactggc
    33781 aataaaccga atgccactgt aattgtcgat gttgatcgtc agggctttgt tgatttactg
    33841 gctgatcgtc tgaaatttta cgcttaacac tcgtcattga ttaagatcac cacgcgttac
    33901 ctgggtggtc ttaatctatt tatctctttt tctttcaaga tataaatgca atggataatg
    33961 cagaactcgc cataggtatc gatctcggca ctaccaatag tttaattgcc gtctggaaag
    34021 acggtgccgc gcaattaatt ccaaataagt tcggtgaata tttaacacca tccataatta
    34081 gcatggatga aaataatcat attttagtcg gaaaaccggc tgtatcacgg cgtacttcgc
    34141 atccggataa aacggcagcg ttatttaaac gtgcaatggg cagtaatacc aactggcggt
    34201 taggcagcga cacatttaac gcgccagaac tgtcctcttt ggtattacgc tcattaaaag
    34261 aagatgccga agaatttctg caacgtccga ttaaagatgt ggtgatctcc gttccggctt
    34321 atttcagcga taaacaacgc aagcataccc gtttagcagc ggacttagcc gggttaaatg
    34381 cggtacgctt aattaatgaa cccacagcag ctgcgatggc gtatggcctg catacccaac
    34441 aaaatacccg ttcgctggtg tttgatctcg gtggcggcac gtttgacgtt acggtgcttg
    34501 agtacgccac gccggtgatt gaagttcacg cctccgctgg cgacaacttc ctcggtggcg
    34561 aagattttac ccatatgttg gtcgatgacg ttttaaagcg cgcggatgtc acaaggacca
    34621 cgcttaacga gagtgaactg gcagccttgt acgcctgtgt ggaagcggca aaatgtagca
    34681 atcaatcgcc aatgcacatt cgctggcagt accaggatga aacgcgggaa tgcgaatttt
    34741 acgagaacga actggaagat ttgtggttgc cgctgctcaa tcgcttgcga gtgccgattg
    34801 aacaggcgtt gcgcgatgcg cgtctgaagc cgagtcaaat cgacagtctg gtgctggttg
    34861 gcggcgcgtc acaaatgccg ctggtgcagc gaatcgccgt gcgtctgttt ggcaaattac
    34921 cgtatcaaag ttacgatccg agcaccattg tcgcgctggg cgcagcaatc caggccgcct
    34981 gccgcttacg cagtgaagat attgaagaag tgatcctcac tgatatttgc ccttactcgt
    35041 tgggcgttga ggttaaccgc cagggcgttt ccggcatttt ctcgccgatt attgaacgaa
    35101 acaccactgt gcccgtgtcg cgtgtagaaa cttattcaac catgcacccg gaacaggatt
    35161 caattacggt taacgtctat cagggagaaa accacaaagt taaaaacaac attctggtgg
    35221 aatccttcga tgtgccgttg aagaaaaccg gggcttatca gtcgattgat attcgcttta
    35281 gttatgatat caacgggttg cttgaagttg acgtgcttct ggaagacggc agcgttaagt
    35341 ccagagtgat taaccacagc ccggtaacat tgagcgcgca gcagattgaa gagagtcgga
    35401 cgcggttatc cgcattgaaa atttatccgc gcgatatgct catcaatcgc acctttaaag
    35461 ccaaactgga agagttgtgg gcgcgggcgc tgggtgacga gcgagaagag attggccggg
    35521 tgatcaccga ttttgatgcg gcgctgcagt caaacgatat ggcccgcgtc gatgaggtca
    35581 ggcggcgggc gagcgtttat ttagccattg agacctcata atcacggctg ccggatgcgg
    35641 cgtaaatgct gcatccggca tcggcgcaaa aaacacatcg tcaactaaac aacatcgcga
    35701 ttttgccgag agcataacct ataaccagca ccgttaaact gattttgata atgctggtaa
    35761 tgctctgtgg tttttcaccc tcatcatttt tttcttcatg gttttcaggt gttggctcat
    35821 cgacatcttc atcctccggc tcgagtttta ctttcgtcaa cagcatccgg gtgataatgg
    35881 cctctaatgg atgagcatct ccgccttcca gccttgtatt aagacgggcc agcatctctt
    35941 gtttcggaaa cgcttcaagt actacctgtt ccgctacccg atacaaatcc agtaacggtg
    36001 aatcttcttc tgtctcgcta ccgactctcg atttccacca cgcccatgct ttgcgtttga
    36061 agaataacga ctcaataaac gattgaccta acggcgataa ttttccgcta tgtaaaattt
    36121 ccgcccataa cagccgcgtc cgggcgctct caccaggaac atatttcagc ggttgacctt
    36181 gcgcatctac catgctccgc gcttcgataa ccatcactaa taacgcgaaa taatccgggc
    36241 aatgacgctt gctccactgc aacaacaaat tatcagcttg tgtagatggg taactttccc
    36301 agtacgcgcc gagatcagca agcaggcttt ccccctcgcc gcagtacaaa cgttgtgcac
    36361 actggtaata ccgggcatct tcgctttctg gttcttccgc ttgccactgt ttggctacag
    36421 gaatgagttc caccatacct gtttgcaccg agctgtacca gcgcaacagt ttgcgatgta
    36481 actttgcatc atcaggaatg aaccacggtc cttccatcgc caaaaacgca gtgacatagt
    36541 caggatggta acggcagcaa cgttccagcg cgaagtaata ttcaacagtc tggttctgta
    36601 acgcgacggg atattccaga aggctaagga aatcgaaaca atcaccagcc tgaatggcct
    36661 caagaaaagc ctccctttct tcctcatcca ttccttcgct gtcattaaac gaatgccagt
    36721 tcaaacgctc tgccaacaaa gaagcacaat ttaatgacag atatcgcgct tctatagcga
    36781 ttgcacacag cggccagcgt aattgatcga catcctccat gttccaggta tttaattgct
    36841 gaataaattt ctgccaggcg gagggctgaa aacgatcact ttctgaatcc agtaatgtcc
    36901 tgaatgcacg tagtatttca tgttcagcgg tggcatcttt ttcttcatca tctgcttcct
    36961 cgacaggatt taccgccagt cgcagcgcct cttcataggc ctggcgtaat gctttaaacc
    37021 cctgcggatc ggtttcggga tgacacaacg gtaagcgagc aagataagcc tggcggataa
    37081 tgtctatttg cgtcgtgctt tcaatttcga ggatttgcca acatgttttc ataggtcgct
    37141 cacatcctta agcgttaccg ggcattcagg ctggacagtt aatggctgcc agggaagcgt
    37201 ggcatagaaa cggtcgcttc ccgctgccag ctcttcgaac atccgactcc cggcgctatc
    37261 gtatttgtac agggttagcg cctcgcgtag cgaagcgttg tcttcgcact gagaagattg
    37321 ccagtagaga cgccctagtg aataggcaga aaaatagtgc atccagctgt gatagtaatg
    37381 gtgagctcgt gcatagacgc gcgattgcag ccataaactc tcttcttccg tcagccagtt
    37441 gttgagcaca ccggcccgtg acagaaaccc cattcgaaca taatcccagg ctttgattcc
    37501 cccttctcca cagcacatag cggtttgggc aacgaattgg gtataaatca accctctctc
    37561 cgagccacca gcaattaatg ctttccactc ttcaggggaa gagcgttgcc atgtcagata
    37621 taagccagct aaatcattgg catgaccatc atcagtcatc tgataaatag tctgaattaa
    37681 atcatcacga ttatttattc cccagga
//
LOCUS       DAJTXY010000021        37648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTXY010000021 DAJTXY010000000
VERSION     DAJTXY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.021"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..175)
                     /locus_tag="OO566_001240"
     CDS             complement(<1..175)
                     /locus_tag="OO566_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6604594.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO566_001241"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="OO566_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6604595.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO566_001242"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="OO566_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6604596.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO566_001243"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="OO566_001243"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6604597.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO566_001244"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="OO566_001244"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6604598.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO566_001245"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="OO566_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6604599.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="OO566_001246"
     CDS             complement(5179..5421)
                     /locus_tag="OO566_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6604600.1"
                     /translation="MDTSFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO566_001247"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="OO566_001247"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6604601.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHIEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO566_001248"
                     /gene_synonym="fabE"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="OO566_001248"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6604602.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="OO566_001249"
     CDS             complement(8338..9312)
                     /locus_tag="OO566_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6604603.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="OO566_001250"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="OO566_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6604604.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="OO566_001251"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="OO566_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6604605.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="OO566_001252"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="OO566_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6604606.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="OO566_001253"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="OO566_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6604607.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="OO566_001254"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="OO566_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6604608.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="OO566_001255"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="OO566_001255"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6604609.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="OO566_001256"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="OO566_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6604610.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVDGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="OO566_001257"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="OO566_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6604611.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFSYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO566_001258"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="OO566_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6604612.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /locus_tag="OO566_001259"
     CDS             23179..23382
                     /locus_tag="OO566_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6604613.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="OO566_001260"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="OO566_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6604614.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="OO566_001261"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="OO566_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6604615.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="OO566_001262"
     CDS             26387..26659
                     /locus_tag="OO566_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6604616.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTLGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO566_001263"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="OO566_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6604617.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO566_001264"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="OO566_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6604618.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="OO566_001265"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="OO566_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6604619.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO566_001266"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="OO566_001266"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6604620.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31772)
                     /gene="degQ"
                     /locus_tag="OO566_001267"
     CDS             complement(30405..31772)
                     /gene="degQ"
                     /locus_tag="OO566_001267"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR6604621.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDEKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTNTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31926..32324)
                     /locus_tag="OO566_001268"
     CDS             complement(31926..32324)
                     /locus_tag="OO566_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6604622.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32518..33645
                     /gene="zapE"
                     /locus_tag="OO566_001269"
     CDS             32518..33645
                     /gene="zapE"
                     /locus_tag="OO566_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6604623.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33864..34292
                     /gene="rplM"
                     /locus_tag="OO566_001270"
     CDS             33864..34292
                     /gene="rplM"
                     /locus_tag="OO566_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6604624.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34308..34700
                     /gene="rpsI"
                     /locus_tag="OO566_001271"
     CDS             34308..34700
                     /gene="rpsI"
                     /locus_tag="OO566_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6604625.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35095..35733
                     /gene="sspA"
                     /locus_tag="OO566_001272"
     CDS             35095..35733
                     /gene="sspA"
                     /locus_tag="OO566_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6604626.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35739..36236
                     /gene="sspB"
                     /locus_tag="OO566_001273"
     CDS             35739..36236
                     /gene="sspB"
                     /locus_tag="OO566_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6604627.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36279..37646)
                     /gene="dcuC"
                     /locus_tag="OO566_001274"
     CDS             complement(36279..37646)
                     /gene="dcuC"
                     /locus_tag="OO566_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6604628.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9115 a         9415 c         9905 g         9213 t
ORIGIN      
        1 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 accaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgtggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atagatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcaa accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaagggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccctatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagc gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggttaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaaccggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcggttt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gctaggtgca taccgaacat caggattgcg actacaatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagcgtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcatctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     5401 ctgaacaaaa ctagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg atgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taactttgta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgcgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaagataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccttggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtgcttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 caggactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat acggctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctccc cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgactttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatggaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgc gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggaaccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgcc
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcata
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgcc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcactgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttgacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gatatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag agcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc gggcagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttgacgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacatt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaagct cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgtcga gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggatgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcgtaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttagt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg acgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactcta accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gctgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt aatgagattg agagtcagga ggatttttat cgtgacttta gccaaaccct
    26461 tggtctggct aaagataagg tacgcgatct tgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa gcccccgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggc cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagcatacac taattattga
    27061 actaattaat accacgctaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcgaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggcgtg
    30661 atcatttcag cgctggccga cgaagaggtg ttggtatcaa gcgtcacttc tacttccagt
    30721 ggtttgccgt tacgcagcag gccaagcttc actttcgtgc ccggctctgt agtcgcgata
    30781 cgagagcgca actcagcaaa gctattcagc ggtttgccgt tgaggctggt aataatatcg
    30841 cccgctttga cgcccgcttt cgctgagcca gaacttggca acacttcgct gacaaacgcg
    30901 ccacgctgca cgtcaaggtt gaaggctttg gcgatatcgg cactcatctc ggtgcctttg
    30961 atgcctaaca aaccgcgttt gatttcacca aagtcgataa gctgctgcgc cagtgttcgc
    31021 gccatattac tggggatggc aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt
    31081 gcagtgttga tgccaattaa ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg
    31141 cggttaatgg aagcatctgt ctggataaag ttttccagac cttcaagatt caacccgctg
    31201 cggcctaatg cggaaacaat gccagaggtg gcggtttgcc caaggccaaa tgggttaccg
    31261 accgctacgg caaaatcacc gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt
    31321 aatttgctcg ggttttgaat ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc
    31381 agtttttcat caaactcgcg cccatcattg agctgaatac tgattttctg tgcctgatta
    31441 atcacatggt tgttggtcag cacatagcct ttactggcgt tgatgatgac accggaacct
    31501 aaaccttcga agggttgtgc aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat
    31561 tcttccggga ttttctgtcc ctgactggcc gttccttcca cccgtacgct caccactgcc
    31621 ggaagcactt tttccagcat tggagccaga ctggggagag gggcctgatc ggcaacctgg
    31681 cctggaatcg acgcgacggc ctgaaatgac gccgagagag ttaacccggc acttaacgct
    31741 aatgcactca acagctgggt ttgttttttc attattcctg ctctcgtacc tgaatgataa
    31801 gaaaagagat tcaaaacagt ttgatgttat tgaattttca gacggctaac aatgagacac
    31861 cagattaaat gatagtcgtg gagggagaga agaggagggc gcaatggctg cgcccgaaaa
    31921 ataaattagt cgcgcttcgc gccagtacgc agcaggccgg atgcgccttc agaatagtcg
    31981 cgaggcatct gcaccggtgc ctgatcgttg ctggcttcag actctgccag acgattacgg
    32041 aacgggtttg cttcagcaga cagttccggc agcaggctgc tggagctttt tgccatgtgc
    32101 tgatacagct ggcgatagtc gtgcgccatg gtatccagta attccgcgct gcgggcaaag
    32161 tggctaacca gctcttcgcg atactcgtcc agttcagctt tattcttttc cagttcgtac
    32221 tgcaacgcct gttgctggcg tagtttacga ttaccaaaac gcatggccac agcaccaata
    32281 atgatgccga cgactaaccc aattagcgca tattcccagg tcatgaacat ctcccgttgt
    32341 cttttgtttc cgtagggtgt tggcttcagg ctccatgcct gcggctgatt atgccactat
    32401 aaccgttaat tccacagaag tggaatcccg actgcatatc gcgtagtgta gaacggcctt
    32461 tttttcgtca accgtgaaca acggcgcacc gattattcaa ggaataacaa taagatcatg
    32521 caaagcgtta ccccaacatc gcaatacctg aaggcgctca atgaaggcag ccatcaaccc
    32581 gacgacgttc aaaaagaggc cgtcagccgc ctggaaatta tttatcaaga actcatcaat
    32641 agcaggccac cagcccccag gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt
    32701 aaacgcgaag acacaaagca tacgccagtg cgtggcttat atatgtgggg cggtgtagga
    32761 cgcgggaaaa cctggctgat ggaccttttc tatcaaagcc tgccgggaga gcggaaacag
    32821 cgcctgcact ttcaccgttt tatgctgcgg gtgcacgaag agctaactgc cttacagggg
    32881 cagaccgatc cgctggaaat tattgccgat cgctttaaag ccgaaactga cgtgctctgt
    32941 tttgacgaat tttttgtttc tgatattacc gacgccatgc tacttggcgg tctgatgaaa
    33001 gccctgttca cccgcggtat taccctggta gcgacgtcaa atattccgcc ggacgaactt
    33061 tatcgaaatg gcctgcaacg tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat
    33121 tgtgatgtaa tgaacgtgga cgctggtgtt gattatcgac tgcgtacact cactcaggcg
    33181 catctgtggc tttcgccact caacgatgaa acccgggcgc agatggataa actatggttg
    33241 gcgctggcgg gggtgaaacg agaaaattca ccgacgttag aaatcaacca tcggccattg
    33301 gcgacaatgg gcgtcgagaa ccagacgctg gcggtttctt ttactacgct gtgcgtcgac
    33361 gcccgcagtc agcatgacta tattgcgctc tcacgcctgt ttcacacagt aatgttgttt
    33421 gatgtgccag tcatgacgcg gttgatggag agcgaagcgc ggcgctttat tgcgctggtg
    33481 gatgagtttt acgagcgcca tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa
    33541 atttatcagg gtgagcggct gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag
    33601 atgcaaagcg aagagtatct gaagcgcgag catttggcgg gttaaaacct gccacaaatc
    33661 acaaaaaggg gtcgatcttt gaccccgact tctctataat cctgcgaccc cacgttacaa
    33721 gaaagttttt ttcccaaaac tttttgtgtg ctggcatagg ctattcgaag gggtaggttt
    33781 gccggacttt gtcgtgtgaa cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta
    33841 actatttatt gggtaagctt ttaatgaaaa cttttacagc taaaccagaa accgtaaaac
    33901 gcgactggta tgttgttgac gcgaccggta aaactctggg ccgtctggct actgaactgg
    33961 ctcgtcgcct gcgtggtaag cacaaagcgg aatatactcc gcacgtagat accggtgatt
    34021 acatcatcgt tctgaacgct gacaaagttg ctgtaaccgg caacaagcgt actgacaaag
    34081 tgtactatca ccacaccggc cacatcggtg gtatcaaaca agcgaccttt gaagagatga
    34141 ttgctcgccg tcctgagcgt gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc
    34201 cgctgggtcg tgctatgttc cgtaaactga aagtttacgc gggtaacgag cacaaccacg
    34261 cggcacagca accgcaagtt cttgacatct aatcgggatt ataggcaatg gctgaaaatc
    34321 aatactacgg cactggtcgc cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca
    34381 acggtaaaat cgtaatcaac caacgttctc tggaacagta cttcggtcgt gaaactgccc
    34441 gcatggtagt tcgtcagccg ctggaactgg tcgacatggt tgagaaactg gacctgtaca
    34501 tcaccgttaa aggtggtggt atctctggtc aggctggtgc gatccgtcac ggtatcaccc
    34561 gcgctctgat ggaatacgac gagtccctgc gttctgaact gcgtaaagct ggcttcgtta
    34621 ctcgtgacgc tcgtcaggtt gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc
    34681 cgcagttctc caaacgttaa ttggcttctg ctccggcaga aaacaatttg cgaaaaaacc
    34741 cgcttcggcg ggttttttta tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct
    34801 ttttgctgcc gtctgaaaga caggtaaaac aaaaaccaca tcgccaataa gggtctaagt
    34861 caactatttc agactaaagc gcatctcttt ttccccattt ccggcatcca ctcaccacaa
    34921 aggtcgcaaa atctggtaaa ctatcatcca attttctgcc caaatgtcgg gtattgctca
    34981 ttttttgttt gattttcgaa caaagagagt cgttccttat tgggtaacac aacttctgac
    35041 tggccacctg gtggctggta gcagtaaaaa ttctgactat acctggaggt tttcatggct
    35101 gtcgctgcca acaaacgttc ggtaatgacg ctgttttccg gtcctactga catctatagc
    35161 catcaggtcc gcattgtgct ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa
    35221 aaggacaatc cgcctcagga tctgattgac cttaacccga atcagagcgt tccgaccctg
    35281 gtggatcgtg agctgaccct gtgggaatct cgcatcatta tggaatatct ggatgagcgt
    35341 ttcccgcatc cgccactgat gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac
    35401 atgcatcgca tcgaaaaaga ctggtacacg ctgataaaca ctatcatcaa cggttcagct
    35461 tctgaagcag atgccgcacg taagcaactg cgcgaagaac tgctggcgat tgcgccggtc
    35521 ttcggtcaga agccgtactt cctgagcgat gagttcagcc tggtcgattg ctaccttgct
    35581 ccgctgctgt ggcgtctgcc gcaactgggc atcgagttca gcggcccggg tgcgaaagag
    35641 ctgaaaggct atatgacccg cgtctttgag cgtgactctt tccttgcttc tttaactgaa
    35701 gcagaacgtg aaatgcgtct gggccggagt taatctgtat ggatttgtca cagctaacac
    35761 cacgtcgtcc ctatctgctg cgtgcattct atgagtggtt gctggataac cagctcacgc
    35821 cgcacttggt ggtggatgtg acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg
    35881 acgggcaaat cgtactcaac attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg
    35941 atgaggtgcg ctttaacgcg cgctttggtg gcattccgcg tcaggtttct gtgccgctgg
    36001 ctgccgtgct ggctatctac gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag
    36061 ctgcctacga tgaagatacc agcatcatga atgatgaaga ggcatcggca gacaacgaaa
    36121 ccgttatgtc ggttattgat ggcgacaagc cagatcacga tgatgacact catcctgacg
    36181 atgaacctcc gcagccacca cgcggtggtc gaccggcatt acgcgttgtg aagtaataca
    36241 aaacaggccc aggcggcctg ttttgtcttt ttaatgactt aataaaatag cgtgattgtg
    36301 gcaatcatat tcaccacgaa accgactgcc atggggatcg ctgtccgctt caccaccgca
    36361 aaaggcgaaa cgcctgcaat tcctgacgta acgaccacca ccgcagtaat cggcgaaacc
    36421 gcgcgcgcta gcgtcgtggc aaaatgcatc ggcataatca ttacaaccgc tggtacatgt
    36481 agtccggctg cgatattcgg aataagactg gcaaatgaca taaacggcgc attgccagag
    36541 cccatcacaa tggcacaaat ggcaatgact agcgccataa taatcatcac gccaataccg
    36601 cccagaccag aatgctccgc acccctgata accgcatcaa cagtgccaat cgtcgttaag
    36661 cctttcgcaa atatttcacc cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc
    36721 atgccgtcaa aaaacgcctg cacatcgtcc attgtttcgc gcaagttatg cttgcggaag
    36781 aactcaacaa acatcgtcac cgtcaaactt agtaacatca ccaccaccag atgcagttct
    36841 gacttcatca gcccgacgtg ggcgaggaac agcgagccga gcatcaggat taacggcatc
    36901 acaggtaaaa tggcgtaata gagcggcggg acattatcga gagttttttg ctctgcctgt
    36961 tcgtgattga tatttttatc ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc
    37021 gccaccgaga taatgacgca agaggcgacc ggaagctggt agtggaagaa gtatgtggca
    37081 attttcattc ccgcgacctg ggctgcaaaa atggagttcg tttccagaat cccccactca
    37141 atggacatcg tggttgcgat aaccgccacc gcagagaggc gacttactcc cagactcacc
    37201 agcgtcggaa ataaggtgac catcagcaac atacccagac cggaggcgct ggtaataaac
    37261 tgcgccatga tttggccgat gacgtaagtt gccgacagaa taatatacgg cgagcgaatg
    37321 agttttaacg ggcggcttag caagctcacc atcgcgcgac tggctcctat gcgctccatg
    37381 tagcgggcat aaccgcccac cgccataatc gacagcccca atccggcaat gcggttggac
    37441 agcatgcgca ggatttcatt ataaatgtcg acgatcaagt agccgctgct tttggcggga
    37501 tcgagtacac caccgaaccc taaccagact ccgcacatca tcaggaagac acctgcggca
    37561 gccagcacca cctgaggttt gtagtttttc aggatcaaat agcccatcgt aattaatacg
    37621 atgacagata taattatgcc gaacattg
//
LOCUS       DAJTXY010000022        36608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTXY010000022 DAJTXY010000000
VERSION     DAJTXY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.022"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            27..2294
                     /gene="adiA"
                     /locus_tag="OO566_001275"
     CDS             27..2294
                     /gene="adiA"
                     /locus_tag="OO566_001275"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6604629.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2619..3380
                     /gene="adiY"
                     /locus_tag="OO566_001276"
     CDS             2619..3380
                     /gene="adiY"
                     /locus_tag="OO566_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6604630.1"
                     /translation="MRICSDQPCIVLLTEKDVRIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3517..4854
                     /gene="adiC"
                     /locus_tag="OO566_001277"
     CDS             3517..4854
                     /gene="adiC"
                     /locus_tag="OO566_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6604631.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4958..6601
                     /gene="eptA"
                     /locus_tag="OO566_001278"
     CDS             4958..6601
                     /gene="eptA"
                     /locus_tag="OO566_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6604632.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6598..7266
                     /gene="basR"
                     /locus_tag="OO566_001279"
     CDS             6598..7266
                     /gene="basR"
                     /locus_tag="OO566_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6604633.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7267..8367
                     /gene="pmrB"
                     /locus_tag="OO566_001280"
     CDS             7267..8367
                     /gene="pmrB"
                     /locus_tag="OO566_001280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6604634.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="OO566_001281"
     CDS             complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="OO566_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6604635.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8544..10046)
                     /gene="proP"
                     /locus_tag="OO566_001282"
     CDS             complement(8544..10046)
                     /gene="proP"
                     /locus_tag="OO566_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6604636.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10310..11188)
                     /locus_tag="OO566_001283"
     CDS             complement(10310..11188)
                     /locus_tag="OO566_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6604637.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNSGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="OO566_001284"
     CDS             complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="OO566_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6604638.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13815..14150
                     /locus_tag="OO566_001285"
     CDS             13815..14150
                     /locus_tag="OO566_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6604639.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14308..14751
                     /gene="yjdN"
                     /locus_tag="OO566_001286"
     CDS             14308..14751
                     /gene="yjdN"
                     /locus_tag="OO566_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6604640.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14884..15672
                     /gene="phnC"
                     /locus_tag="OO566_001287"
     CDS             14884..15672
                     /gene="phnC"
                     /locus_tag="OO566_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6604641.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15697..16713
                     /gene="phnD"
                     /locus_tag="OO566_001288"
     CDS             15697..16713
                     /gene="phnD"
                     /locus_tag="OO566_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6604642.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSSGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16819..17598
                     /gene="phnE"
                     /locus_tag="OO566_001289"
     CDS             16819..17598
                     /gene="phnE"
                     /locus_tag="OO566_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR6604643.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17619..18344
                     /gene="phnF"
                     /locus_tag="OO566_001290"
     CDS             17619..18344
                     /gene="phnF"
                     /locus_tag="OO566_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR6604644.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18345..18797
                     /gene="phnG"
                     /locus_tag="OO566_001291"
     CDS             18345..18797
                     /gene="phnG"
                     /locus_tag="OO566_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR6604645.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMIRGDNA"
     gene            18794..19378
                     /gene="phnH"
                     /locus_tag="OO566_001292"
     CDS             18794..19378
                     /gene="phnH"
                     /locus_tag="OO566_001292"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR6604646.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19378..20442
                     /gene="phnI"
                     /locus_tag="OO566_001293"
     CDS             19378..20442
                     /gene="phnI"
                     /locus_tag="OO566_001293"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR6604647.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20435..21280
                     /gene="phnJ"
                     /locus_tag="OO566_001294"
     CDS             20435..21280
                     /gene="phnJ"
                     /locus_tag="OO566_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR6604648.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21277..22035
                     /gene="phnK"
                     /locus_tag="OO566_001295"
     CDS             21277..22035
                     /gene="phnK"
                     /locus_tag="OO566_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR6604649.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22146..22826
                     /gene="phnL"
                     /locus_tag="OO566_001296"
     CDS             22146..22826
                     /gene="phnL"
                     /locus_tag="OO566_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR6604650.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22823..23959
                     /gene="phnM"
                     /locus_tag="OO566_001297"
     CDS             22823..23959
                     /gene="phnM"
                     /locus_tag="OO566_001297"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR6604651.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPTSLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            23959..24516
                     /gene="phnN"
                     /locus_tag="OO566_001298"
     CDS             23959..24516
                     /gene="phnN"
                     /locus_tag="OO566_001298"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR6604652.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLLVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24503..24937
                     /gene="phnO"
                     /locus_tag="OO566_001299"
     CDS             24503..24937
                     /gene="phnO"
                     /locus_tag="OO566_001299"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR6604653.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24939..25697
                     /gene="phnP"
                     /locus_tag="OO566_001300"
     CDS             24939..25697
                     /gene="phnP"
                     /locus_tag="OO566_001300"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR6604654.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRAQRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(25844..26173)
                     /locus_tag="OO566_001301"
     CDS             complement(25844..26173)
                     /locus_tag="OO566_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604655.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26242..26874)
                     /locus_tag="OO566_001302"
     CDS             complement(26242..26874)
                     /locus_tag="OO566_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6604656.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGCCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26955..29170)
                     /locus_tag="OO566_001303"
                     /pseudo
     CDS             complement(26955..29170)
                     /locus_tag="OO566_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            29284..30804
                     /locus_tag="OO566_001304"
     CDS             29284..30804
                     /locus_tag="OO566_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6604657.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30801..31775
                     /locus_tag="OO566_001305"
     CDS             30801..31775
                     /locus_tag="OO566_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6604658.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            31806..32747
                     /locus_tag="OO566_001306"
     CDS             31806..32747
                     /locus_tag="OO566_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6604659.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            32802..33485
                     /locus_tag="OO566_001307"
     CDS             32802..33485
                     /locus_tag="OO566_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HCR6604660.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQRLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            33500..34360
                     /locus_tag="OO566_001308"
     CDS             33500..34360
                     /locus_tag="OO566_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HCR6604661.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            34357..35358
                     /locus_tag="OO566_001309"
     CDS             34357..35358
                     /locus_tag="OO566_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6604662.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(35355..36061)
                     /locus_tag="OO566_001310"
                     /pseudo
     CDS             complement(35355..36061)
                     /locus_tag="OO566_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            36352..>36608
                     /locus_tag="OO566_001311"
     CDS             36352..>36608
                     /locus_tag="OO566_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604663.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYR"
BASE COUNT         8529 a        10227 c         9718 g         8134 t
ORIGIN      
        1 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
       61 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
      121 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
      181 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
      241 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
      301 gcgatcggga aaaagccctc gccgcaatgg accgggatct gctggagctt gtcgatgaat
      361 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt gccgcgatga
      421 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagtgaca
      481 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
      541 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gacatgggca
      601 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
      661 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtacttccg
      721 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
      781 gtaactgcca taaatccatc gaacaaggtt tgatgctgac aggcgcgaaa ccggtctata
      841 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
      901 ctgaaacctt gcaaaagaaa atcagtgaaa gcccgctgac caaagacaaa gccgggcaaa
      961 aaccgtctta ctgcgtggta accaactgca cctatgacgg cgtgtgttat aacgctaaag
     1021 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     1081 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     1141 atcacaacgg tcctaccgtt ttcgccaccc actccatcca caaactgctg aatgcgctgt
     1201 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     1261 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     1321 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacag gaagtgatta
     1381 acgaagcggt tgatttccgt caggcgatgg cgcggctata taaagagttc accgctgacg
     1441 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     1501 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     1561 tacatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     1621 acccaattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     1681 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     1741 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     1801 ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     1861 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     1921 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     1981 acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     2041 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     2101 actcagttat cccgtatccg ccaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     2161 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     2221 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     2281 gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     2341 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     2401 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     2461 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     2521 atgcatgttt atattcatca tataattata taaccatttc ccggtatcgc tttgctttag
     2581 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     2641 cttgtattgt tttattgact gaaaaagatg tccggataag ggtgaatggg aaagaaccta
     2701 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     2761 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gactacctcc
     2821 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     2881 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     2941 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     3001 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     3061 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     3121 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagttgaaa agcgaaaaca
     3181 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     3241 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     3301 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     3361 gagaacgcac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     3421 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     3481 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     3541 aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg gtcaggtgtt
     3601 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     3661 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgaccca
     3721 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     3781 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgccatggt ggtcattggc
     3841 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     3901 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     3961 cgcgtgcagg ccgttgccac tgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     4021 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     4081 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     4141 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     4201 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     4261 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     4321 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     4381 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     4441 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     4501 atcgtcggta ttttgatgac catcttccag ctcagcagca tttcaccaaa cgcgaccaaa
     4561 gagttcggtc tggtttcttc cgtctcggtc atctttacac tggtgccata tctttacacc
     4621 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     4681 gctgttacta ccattgcctt cctctactgc atttgggccg tggtggggtc cggggcgaaa
     4741 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     4801 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     4861 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     4921 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     4981 accctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     5041 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     5101 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     5161 aagctctttc ttatggctta atcgaacact ggcctgcctg tttattctgg ttggcgcggc
     5221 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     5281 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     5341 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     5401 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     5461 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     5521 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     5581 tcagcgactg gcaaatctgc cgctggtgcg aattggtgaa gacgcgcacc gcaacccgtt
     5641 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     5701 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     5761 cgtggtctat ttccctaata ccgcatcttg cggtacggca acggcagttt cagtaccgtg
     5821 catgttctcg gatatgccgc gtgagcacta caaagaagag ctggcacagc accaggaagg
     5881 cgtgctggat atcattcagc gagcgggcat caacgtgctg tggaatgaca acgatggcgg
     5941 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctga
     6001 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     6061 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     6121 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     6181 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     6241 cgactatatt gttgataaag cgattaatct gcttaaagaa catcaggata aatttaccac
     6301 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
     6361 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccaatgc tgctgtggct
     6421 gtcggaggat tatcaaaagc ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
     6481 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
     6541 gacgaagtat taccaggccg cggatgatat tctgcaaact tgcaggagag tgagtaaatg
     6601 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
     6661 accgaaggct acgcgtgcga tggcgtgaca accgcgcgga tggcggaaca aagccttgag
     6721 gacggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
     6781 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
     6841 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
     6901 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
     6961 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
     7021 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
     7081 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
     7141 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
     7201 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
     7261 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
     7321 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg gctatggcat
     7381 gaaagtaccg agcagattca actgtttgag caggcgctac gcgacaatcg caacaacgat
     7441 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
     7501 atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac ccgcccgctg
     7561 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
     7621 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
     7681 ctgaccagca cgctggataa cgaaaggttg tttaccgctg acgtcgcgca cgaactgcga
     7741 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
     7801 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
     7861 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
     7921 gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
     7981 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
     8041 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccgcaagg cagcaacatt
     8101 atgattaagc tgcaagaaga tggcggagcg gtcatggcag ttgaagatga aggaccaggt
     8161 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
     8221 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
     8281 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
     8341 gatcagaacg tggcaaacca gatataaaga aagctgctga ccaccagcac gctgaacacg
     8401 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
     8461 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
     8521 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
     8581 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat tatcgatttt ctgctcgata
     8641 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
     8701 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
     8761 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
     8821 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
     8881 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
     8941 ataaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
     9001 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagtgcaac actaccaagt
     9061 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     9121 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     9181 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     9241 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     9301 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     9361 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     9421 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     9481 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     9541 tccagccagc tgcccataaa gccacgttta cggtcggggg agtattccgc aacaaatatc
     9601 gacgccccgg tatattcacc accgaccgag aaaccttgtg ccatcttaca gatcaacagc
     9661 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     9721 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
     9781 aacataccaa agaagagtcc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
     9841 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
     9901 taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc cagtgatgct
     9961 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    10021 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttgaccca tttccgcaaa
    10081 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    10141 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    10201 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    10261 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    10321 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    10381 gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg ggttgtttat
    10441 ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg cccgtcggaa
    10501 cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
    10561 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagcaacaac accaccgctt
    10621 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
    10681 taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa
    10741 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
    10801 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
    10861 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
    10921 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
    10981 gcagactaag cggcgcattt aacatgccgc tgttcgtcca tgtctgaagc tgcacacgcg
    11041 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttga ggatggcggg
    11101 catcatcgcc ctgcgccaga tccactaaaa agcgcggatg aaccgactcc agcacgcgac
    11161 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
    11221 atcgcgtaac agctgagcgt cggtgtaaat ccagctgtga gtcatcacag tctgctgcaa
    11281 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca gactttcgcg
    11341 taaacgcgtc tgtaattctg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
    11401 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaatagag
    11461 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
    11521 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
    11581 aacgcctaaa cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga cttcactgaa
    11641 cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg
    11701 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc
    11761 ggttaacacc cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact
    11821 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct cgcttaacgg
    11881 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc accgcatctt gctgttgacg
    11941 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
    12001 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
    12061 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
    12121 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
    12181 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
    12241 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
    12301 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
    12361 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
    12421 gaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
    12481 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
    12541 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    12601 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    12661 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    12721 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    12781 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    12841 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    12901 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    12961 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    13021 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    13081 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    13141 accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag
    13201 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc
    13261 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    13321 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    13381 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    13441 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
    13501 agaaataatt ttatatttag tgtgttgttt tttatcagaa taaataacgt cttctgatgc
    13561 gtttaaaacg tcagaaagat aaaaatatca tgtaaataaa aaaaagaaca agtagagcat
    13621 taacattatc ttaaataata aatagaggca aaaagattat tttctttttg cgtttccttt
    13681 caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt
    13741 gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt
    13801 ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacttacg
    13861 aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg
    13921 cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg
    13981 acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg ctgaaaattg
    14041 gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg
    14101 acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga ttgtattgtg
    14161 atcggtaagc cgaataaggc gctcgcgccg catccggcaa cgatgccgac tgcctgatgc
    14221 gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac tacacttaac
    14281 tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt
    14341 aactgttccg acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa
    14401 atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg ccgagaactg cccttccgga
    14461 atgcaatttc ccgataccgc catcgctcat gccaacgtgc gcattgccgg aagcgacatc
    14521 atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac gctggtgctc
    14581 gattcgcaac aggtcgaaga aggaaaacgc tggtttgata accttgccgc taacggaaaa
    14641 atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa
    14701 tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata acccgccggg
    14761 aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt
    14821 taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc
    14881 atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaagcg
    14941 ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg tggctctgct tgggccgtcg
    15001 ggttccggca aatccaccct tttacgtcat ttaagcggtt tgattaccgg cgataaatcc
    15061 gccggtagcc atatcgagct actgggccgc actgtccagc gcgaaggccg cctggcccgc
    15121 gatatccgca aaagccgcgc caacaccggc tacatctttc aacaattcaa cctggtgaac
    15181 cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc gttctggcgc
    15241 acctgtttta gctggttcac cggcgagcag aaacagcgcg cgttacaggc gctgacccgc
    15301 gttggcatgg tgcattttgc ccatcagcgc gtttccaccc tctccggcgg ccagcagcaa
    15361 cgtgtggcga ttgcccgtgc gctgatgcag caggcgaaag tgattctggc cgatgaaccc
    15421 atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg acaccctgcg cgacatcaac
    15481 cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc cctgcgctac
    15541 tgcgaacgca tcgtcgccct gcgtcagggg cacgtcttct acgacggcag cagccaactg
    15601 tttgataacg aacgttttga ccatctctac cgcagcatta accgcatcga agagaacgcg
    15661 aaagctgcct gatatcccca tcattgagga aaacgaatga acgctaagat aattgcctcg
    15721 ctggccttca ccagcatgtt cagcctcagc accctgttaa ccccggcaca cgccgaagag
    15781 caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa cctgaaaccg
    15841 caatggacgc cgtttttaca ggatatggag aagaaactgg gcgtgaaggt caacgccttc
    15901 tttgccccgg actacgcggg cattatccag gggatgcgct tcaataaagt ggatattgcc
    15961 tggtacggca acctgtcggc gatggaagcg gtggatcgcg ccaatggcca ggtcttcgcc
    16021 cagacggtcg cggcggatgg atcgtcgggt tactggagcg tgttgatcgt caacaaagac
    16081 agcccgatca acaacctgaa cgatctgctg gcgaagcgga aagatctcac cttcggcaat
    16141 ggcgatccta actccacctc tggcttcctc gtccccggtt actacgtctt cgctaaaaac
    16201 aatatctccg ccagcgactt caagcgcacc gtcaacgccg ggcatgaaac caacgcgctg
    16261 gccgtcgcca acaagcaggt ggatgttgcc accaacaaca ccgaaaacct cgacaagctg
    16321 aaaacctccg cgccggagaa gcttaaagag ctgaaggtga tctggaaatc gccgctgatc
    16381 ctaggcgatc cgatcgtctg gcgcaagaat ctgtccgaaa ctaccaaaga caagatctac
    16441 gacttcttta tgaactacgg aaaaacgccg gaagagaaag cggtgctgga acgcctaggc
    16501 tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca gctcgcactg
    16561 tttaaagaga tacaaagcgt gaaaggcaat aaagggctga atgagcagga caagctggcg
    16621 aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa cgcgttaagc
    16681 gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag acgccccggc
    16741 gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc ttatccggcc
    16801 tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg tagctggttc
    16861 tcgcttctga gctgggccgt tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa
    16921 atggccccgc ttacgctgat taaagacggc ggcaacatgg caaccttcgc tgccgacttc
    16981 ttcccacccg atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa
    17041 atcgccgtct ggggcaccgc gctggcagtg gttctctcca tccccttcgg tctgatgagc
    17101 gccgaaaact tggtgccgtg gtgggtttac cagcccgttc gccgcctgat ggacgcctgc
    17161 cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg cctcggcccg
    17221 ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa gctgctttcc
    17281 gaagcggagg aagcgatcga acccggcccg gtggaaggca ttcgcgccac cggcgccaac
    17341 aaactcgaag agatccttta cggcgtgctg ccgcaggtga tgccgctgct gatctcctac
    17401 tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat ggtcggcgcg
    17461 ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc
    17521 gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc tcaacggttg
    17581 cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga
    17641 ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac
    17701 actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga
    17761 accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc
    17821 gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg
    17881 cgcgttttag ccagaacctg ctggaccagg gcagccatcc caccagcgaa aaactgctgt
    17941 cggtattgcg ccccgcgtcc ggtcacgttg ctgacgcgct ggggattacc gagggcgaga
    18001 acgttatcca tttgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt ttaatcgacc
    18061 actactttgc ggacctcacc ctttggccga cgctgcaacg cttcgacagc ggttcgctgc
    18121 acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg cggatcagcg
    18181 cccgccgcgc ccaggccaaa gagtgccagc gccttgaaat cccgaatatg tcgccgctgc
    18241 tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca
    18301 gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg
    18361 cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa ctggcagccc
    18421 gcctgaacgc gctgaacatc accgccgact atgaagtgat ccgcaccgcc gaaaccggcc
    18481 tggtacagat tcaggcgcga atgggcggca ccggcgaacg cttttttgcc ggcgacgcca
    18541 cgctgacccg cgccgccgta cgcctgactg acggcaccct cggctacagc tgggtgctgg
    18601 ggcgtgataa acagcacacc gaacgctgcg cgctgattga tgcgctgatg cagcaatctc
    18661 gccactttca aaacttatca gaaaccctta ttgccccgct ggacgctgac cgtatggcac
    18721 gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt acgatgattc
    18781 gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg atgcccagca
    18841 cagttttcgt cgcctgttaa aggccataag cgagccgggc gtgattgtcg ccctgcatca
    18901 gctcaagcgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc
    18961 tgataacgac acgccggtgt ggcttgccgc gccattgagt aacgatatcg tcaatcagag
    19021 cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga ccttcgcggt
    19081 gacggatgac ggaatttcca gcaaacagct caacgccctt tccaccggca ccgccgttgc
    19141 gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg ggcgcatgtt
    19201 gcgtctcacc ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc tgccggagtg
    19261 cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct
    19321 gacctgcggc gaacgcctgc tggctattcc gcgaaccact catgtggagg tgtgctgatg
    19381 tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc
    19441 cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccaaattga acagcagctt
    19501 aatctcgcgg tggatcgcgt gatgaccgaa ggcggcgttg ccgaccgcga actggcggcg
    19561 ctggcgctga aacaggccag cggcgataac gttgaagcaa ttttcctgct gcgcgcctac
    19621 cgcaccacgc tggcgaaact ggcggtaagc gagccgctcg acaccaccga gatgcgtctc
    19681 gaacggcgta tttcggcggt ttataaagac attcccggcg gccagctgct tggcccaacc
    19741 tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggtga agcgccgacg
    19801 ctgaccaccg ccgacagcga acaacagccg tcgccgcacg ttttcagcct gctggcgcgt
    19861 caggggctgg cgaagtttga agaagatagc ggcgcacagc cggatgacat cacccgcacg
    19921 ccgccggttt acccctgctc acgctcctcc cgcctgcaac aattaatgcg cggggacgaa
    19981 ggctatttgc tggcgctggc ctactccacc cagcgtggtt acggacgcaa tcacccgttc
    20041 gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga gctgggattt
    20101 gcggtaaacg tcggcgaact gctgatgacc gagtgtgaaa tggtcaacgg ttttatcgac
    20161 ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtattcgg catgagcgag
    20221 cgcaaagcga tggcaatggc gctggttgac cgtgcgttgc aagccccgga atacggcgag
    20281 cacgcgacag gcccggcgca ggatgaagag ttcgtgctgg cgcatgccga caacgtcgaa
    20341 gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca agccgaactg
    20401 gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt
    20461 tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa aagcggtggc
    20521 tatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccct acggctgggg
    20581 aaccggcggc attcagctca ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat
    20641 tgaccagggc gcagacgaca ccactaacgc cgtgtcgatt cgcaacttct tcaagcgcgt
    20701 gaccggggtg aacaccactg aacgtacgga cgatgcgacg cttatccaga cgcgccatcg
    20761 catccccgaa acgccgctga ccgaagatca gatagttatt ttccaggtgc cgatccccga
    20821 accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc actatgcacg cgctggagga
    20881 gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac
    20941 cacctatgct tatccggtga aggtgaacgg gcgctacgtg atggacccgt cgccgatccc
    21001 gaaattcgat aacccgaaaa tggacatgat gcccgccctg cagctgttcg gcgcagggcg
    21061 cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg atttcgacga
    21121 tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat cgacccacag
    21181 ctatctcgac gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac
    21241 cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttccgtc
    21301 aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc ttttgattta
    21361 tggccggggg aagtgctggg catcgtcggg gaatccggct ccgggaagac cacgctgctg
    21421 aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcgctacga gaatcgttcg
    21481 ttgtatgcga tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg
    21541 catcagcatc cactcgacgg cctgcgtcgc caggtctcgg caggcggcaa tatcggcgag
    21601 cggctgatgg cgaccggggc ccgtcattac ggcgacatcc gcgccaccgc gcagaagtgg
    21661 ctggaagagg tggagattcc cgccaaccgg atcgacgacc tgccgaccac cttttccggc
    21721 ggcatgcagc aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt
    21781 atggacgagc caaccggtgg gctggatgtg tcggtgcagg cacgcctgct cgatctactg
    21841 cgcggcctgg tggtggagct gaacctggcg gtggtgattg tcacccatga tttaggcgtc
    21901 gcccgcctgc tagcggaccg tttgctggtg atgaagcagg ggcaagtagt ggagagtggg
    21961 ttaaccgacc gcgtgctcga cgacccgcac catccgtata cccagctgct ggtgtcatcg
    22021 gttttgcaga attgagccgg tgccggatgc ggcgtaaacg ccttatctgg cctacaaatg
    22081 cgctccccgt aggtcggata agacgcatca gcgtcgcatc cgacacccga accacgaggc
    22141 gaaaaatgat taacgtacaa aacgtcagta aaaccttcat cctgcaccag caaaacggcg
    22201 tgcgcctgcc cgtcctcaac cgcgcctcgc tcaccgtcaa cgcgggcgaa tgcgtggtgc
    22261 tccacggcca ctccggcagc ggcaaatcaa ccctgctgcg ctcgctgtac gccaactatc
    22321 tgcccgatga agggcaaatc cagatcaaac acggtgacga gtgggtagac ctggtcaccg
    22381 cgccagcgcg caaagtggtg gagatccgca aaaccaccgt cggctgggtg agccagtttc
    22441 tgcgcgtcat cccgcgtatt tcagcactgg aagtggtgat gcagccgctg ctcgataccg
    22501 gcgtaccgcg tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc ctgaacgtgc
    22561 cggaacgcct gtggcacctg gcgccatcaa ccttctccgg cggcgaacag cagcgcgtca
    22621 acatcgcccg cggctttatc gtcgactacc cgattctgct gctcgacgaa cctaccgcct
    22681 cgctggacgc caaaaacagc gccgcggtgg tggaactgat ccgcgaagcc aaagctcgtg
    22741 gcgcagccat tgtcggcatc ttccatgacg aagccgtacg taatgacgtt gccgaccgcc
    22801 tgcacccaat ggaagcctct tcatgattat caataacgtc aaattggtgc tggaaaacga
    22861 agtggtacac ggttcactgg aggtgcagga cggcgaaatc cgcgcctttg ccgaaagcca
    22921 gagccgcctg ccggaggcga tggacggcga aggcggctgg ctgctgccgg ggctgattga
    22981 gctgcatacc gataatctgg ataaattctt caccccgcgc ccgaaagtcg actggcccgc
    23041 ccattcggcg atgagcagcc acgacgcgct gatggtggca agcggcatca ccaccgtact
    23101 ggacgccgtg gcgattggtg atgtgcgcga cggcggcgat cggttggaga atctggagaa
    23161 gatgatcaac gccatcgaag agacgcagaa acgcggcgtc aaccgcgccg agcaccgtct
    23221 gctcctgcgc tgcgaactgc cgcatcacac cacgctgccg ctgtttgaaa aactggtgca
    23281 gcgcgagccg gtgacgctgg tgtcgctgat ggaccactcg ccgggccagc gccagttcgc
    23341 caaccgcgag aagtatcgcg aatattatca gggcaaatac tcgctcaccg atgcgcagat
    23401 gcagcagtac gaagaagagc aactggcgct tgccgcacgc tggtcgcagc cgaatcgcga
    23461 atccatcgcc gccctgtgcc gcgcgcgaca aattgcgctc gctagccatg atgacgccac
    23521 ccacgcccac gttgccgaat cccacctgct tggcagcgtg atcgccgaat ttcccaccac
    23581 gtttgaagcg gcggaagcct cgcgcaagca tggcatgaac gtactgatgg gcgcgccgaa
    23641 tatcgtgcgc agcggttcgc actccggcaa cgtggcggcc agtgaactgg cgcagcttgg
    23701 cctgctggat atcctctctt ccgactacta ccccaccagc ctgctggatg cggcatttcg
    23761 cgtcgccgat gaccagagca accgctttac gctgccgcag gcggtgaagt tagtgaccca
    23821 aaatccggcg caggcgctta atcttcagga tcgcggggtg attggcgagg gcaaacgcgc
    23881 ggacctggtg ctggcacatc gcaaggacaa tcatattcat atcgaccacg tctggcgtca
    23941 gggtaaaagg gtgttctgat gatgggaaaa ctgatttggt taatggggcc gtccggctcc
    24001 gggaaagaca gcctgctggc ggaaatccgc ctgcgggaac aaactcagtt actggtggcg
    24061 catcgctaca ttacgcgcga cgccagcgcc ggaagtgaaa accatatcgc cctgagcgag
    24121 caggagtttt ttacccgcgc ggggcaaaat ctgttggcct taagctggca cgctaacagt
    24181 ctgtattatg gcgtcggcgt tgagattgat ctctggctgc acgccggatt cgacgtgctg
    24241 gtcaacggct cacgcgccca tctgccgcag gcgctggcgc gctatcaatc ggcgctgctg
    24301 cccatttgct tactggtttc gccggagatc ctgcgccagc gcctcgaaaa ccgtggtcgt
    24361 gaaaatgcca gtgaaattaa cgcccgacta gcacgcgctg cccgctatac tcctcaggat
    24421 tgtcttacgc tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg
    24481 attcatcaga aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtacga
    24541 caccgacgca gtttacgcgc tgatttgtga gctaaaacag gcggagtttg accaccatgc
    24601 gtttcgcgtg ggctttaacg ccaacctgcg cgacccaaac atgcgctacc atctggcgct
    24661 gctggatggc gaagttgtca gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt
    24721 caactggatc ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg gtctgaacgt
    24781 cggcagtaaa ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac
    24841 cgcgctttcg accaacgtga aacgccacga cgcgcaccgt ttctatctgc gagaaggcta
    24901 cgagcagagt cacttccgct tcaccaaggt gctgtaacat gagcctgacc ctcacgctca
    24961 ccggcactgg cggcgcacag ggcgttccgg catggggctg cgagtgcgcg gcctgcgacc
    25021 gagcgcagcg ctcaccgcag tatcgccgcc agccatgcag cggcgtagtg aagtttaacg
    25081 acgcaatcac cctgatcgac gccgggctgc acgatctcgc cgatcgctgg tcgcccggat
    25141 cgttccagca gtttttgctg acgcattatc atatggatca cgcccagggg ctgtttccgc
    25201 tgcgctgggg cgtgggcgat ccgatcccga tctacggccc gccggatgac cagggttgcg
    25261 acgatctgtt taaacatccg ggcctgcttg atttcagcca cacagtggaa ccgtttgtgg
    25321 tgtttgattt gcaggggttg caggtcacgc cgctgccgct taaccactca aaactgacct
    25381 tcggttatct gctggaaacg gcacatagcc gggtggcgtg gctatctgac accgctggcc
    25441 tgccggaaaa aacgctgaaa tttttacgca ataatcagcc acaggtaatg gtgattgatt
    25501 gcagccaccc gccgcgcgcg gatgcgccgc gtaatcactg tgatttaaat accgtgcttg
    25561 cgctgaatca ggttatccgc tcgccacggg tgattctgac ccatatcagc caccagtttg
    25621 atgcgtggct gatagaaaac gcactgccgt cggggtttga ggcggggttt gatgggatgg
    25681 agattggggt ggcgtgatga gagggaatgt gcgcgctggt cccctcaccc taaccctctc
    25741 cccagagggg cgaggggagt gattgtgctc gatattgaat attgcgctgg ttttctccct
    25801 ctccccattg gggtgagggg cgatgcctgc tccataccca acctcatcgc ccatactcat
    25861 cttccattct ccgctcttca tcttctagct gccgacgctc ctgatcaagc tggcgctggc
    25921 gatcgtccag ctgcctgcgg cgatcttcaa actggcagcg gcggtcgtca tattgtctgc
    25981 gccgatcgtc gctcacttca cgctgccagc cgtcgtcgcg cgaatcttca tagtctcgcc
    26041 cacggtcaga gttataagcg tcattaatcg cctgctgaat attaccaata gtatcgtcga
    26101 taatatcggc ctgggccgga acggtggaca gcgtgagcag ggtgaaaaga aaaagcgtgg
    26161 agtaaggttt cataagccag tctctgcgat ggactggctt aacgttagtt gagtgtgaat
    26221 gaccggagta gcggagtaat ctcaaattcg gtaaggcgca gactcttcca ttagctggtt
    26281 agctcgctgt ttattttccg ccaccaatac aatgccatga catttttgat ccggtgaaat
    26341 actccagata cgctcttccg gaatgcagca gccatcgtag atcactttcg tgccatcgta
    26401 gggccaaaat gccccttgtt cagtgtctaa tacaaacacg gaaatctgat cggactcgcg
    26461 cgcgatcttc tgccagagca tcttcccgcc atcatattga tcaaaatcgc tgattagcgt
    26521 gataccgcat tgcaggatta gcgcttcata cagccgagtt gccagaccct tatcgcgctc
    26581 acctggagag acacaaatac cttccacctg taccagatcc gagtcttccc accagcgttc
    26641 aacggcaatg atatcgctgc cgaactcttt tttacgctgc agatcgagag cggcaagtac
    26701 ccgatgacgc ggcacgcctg caaaaacttc aatgtccgct tcaatgatgg ccgctcttgg
    26761 cgtctggttg tcataaatta cctgtaacag acgtccagaa agctcgatct cttccaccac
    26821 gcgaaaagaa cgagcaaccg tcatagggct ataggtggtt ggcatcacga tcatatgtaa
    26881 atcacttcaa ttcagaacag aattgctcac aatagcagca cgtttgcttc gagcctcttg
    26941 ataaattctg aaaatcatcc ctcaatttgc cccagcacct gcgccagctg cgcgcgggta
    27001 aagggtttgc gcagcagcgc cacgtccggc agcgcggggt tatggctggg gcgtaaatcc
    27061 tgaccgctga tgagtagcag cgtcaggtgc gggtaaagtt tgcgggccgc attgaccact
    27121 tctgcgccgc tcatgccgcc agcaggtcca gcgcctgttc gccgctgctc gcttccagtg
    27181 tcaaatagcc cagcaggtgc aactgctcgc agatggtctg gcgcaccgcg gcttcatctt
    27241 ccagcactaa caccagttta tcgccattaa cagctgtctg ttctaccgcc gctggcgata
    27301 ggtttttcgc cgccgtcagc gcgcggggaa gctgtaatct gaccgttgtc ccctgccccg
    27361 gcgcgctttc aatttccacg cgcccgccgg actggcgcac gaaaccatag accatcgaca
    27421 agcccagccc gctgccgctc cccgtctgtt tggtggtaaa gaacggctcg aacacctggc
    27481 ttttcaccgc ctgcgacata ccgctgccat gatcgatgac ctccagtgcc accatgtcct
    27541 gacgctgccc actgctgcgg gtaacacgct gattccaggt acgaattttg ataattcccg
    27601 tctgcccttc catcgcgtcg cgggcgttca tcaccaggtt gatgatggcg ttttccagtt
    27661 ggctgacgtc aatccacgcg gaccacgccg gagactgcgc ttcaatctca atcgtcagcg
    27721 tcgccggtaa ggagtggcgc attaattcgc ttaactcttc cagcagtgtt tttagttcaa
    27781 cggcttgcgg atgtaaggac tgcttgcggg aaaatgccaa caatcgctgg gtcagtagcg
    27841 cgccacgttc ggcggcttgc tgcgcccgct gtattcgcgg cgcgtcgggt gagttcgtgt
    27901 tcaccagttc caggctacca ataattaccg ccagcagatt attaaaatca tgggccagcc
    27961 cgcccgtcag ttgcctgacc gcttttaact tctggctgtg caatagcgcc tcttccagcc
    28021 cctgccgctc ggtttgggca atctccatct gcgtggtttt ctctttcaac agccgtgacg
    28081 ttcgttcaag tgatgcggta ttgcgggcaa aaacgttaaa ggcgcgcgcc agttcaccca
    28141 actcatcgcg ccgctgtaac gccggaaaag tgacgtccgc ttcgccgtgc gccaggcggc
    28201 tcatcgcgcg ggaaatcgcc gtcaggttag agccgagatt gcgatagata taccaacctg
    28261 caaacgcggt gatcaccacc gctaacaggg cgaaaatcat gatcagcctc tcgccggagc
    28321 gcaaatcctg gtaactttgc tccacccgct gaccagagag cgtcgccacc tgttcggcgt
    28381 aacggttgat gtcgccatta aggatcgcca ccagcgcttt gatgtgaaac atataccagc
    28441 tgattgccag atcgctttgc tccagagccg ccgaaagtgg cgcgagttta cgcagttcac
    28501 gattaaaatc agcgagtagc gtattaacca atggcgtatc gctgtgagtt ggcaaggcag
    28561 acatcactcc gaccagttga tgaataatcg cctgcggcgc gggggtggca atggcggcaa
    28621 caatcagtcg gttcatctct ttaagcaaaa tattatcttg cgcgtgagtg actttttgca
    28681 gatgccgcag ataacttaag ttttgataca gcgaacttaa cagcgcgtta cgctccagat
    28741 gccgcctttg cccgcgttcc agcattccgc cgacgctggt ttgcagttcg ttgctacgcg
    28801 tgatgatgcg cgtcaccagc ccggcttcct gctgcgctaa aggcgcgttc gccagttgtg
    28861 caagcgagct ttttaatgcc cgctgtgtct cctgcaaccg ctcggcttcg cctttatatt
    28921 ccagtgctcc caccacctgc gacaatcgca ctgccgccgt tgccacgttt gccgtgtcgc
    28981 gcgccagatt caggctgccg gtcatatcat ctacagtttg ctgcttcacc agctcttgca
    29041 tctggctggc atgacgaaag cccattaccg ccacgccgct gaccatcaac gtcaccgcca
    29101 ccaccagcaa attcaaaagc agcaatcgcc cgcgcgcgct ggcaaaaaag tgacgtccgg
    29161 ggtgcgccat atcacgctcc gtgaagaaaa atcgcaatat gacaaatatg aacggcttct
    29221 gacattttgc atttactttc ctgtgatgga gtgctgatat cgacaacctc gcaggagtcc
    29281 accatgaaca gcacgccggt tcttgagatg cgcaatattg ccaaagcctt cggcaaattt
    29341 tatgcgctca aaggggtgga tttgacggtc taccctggcg agatccatgc cctgatgggg
    29401 gaaaacggcg cgggaaaaag cacgctgatg aaggtgctgg cgggggcgta taccgccacc
    29461 agcggcgaga tcctgataga cggcaagccc tttcacattc gcacgccaaa agatgcctta
    29521 agcgccggta ttacgctgat ttatcaggag atgcagctgg cacccaattt gacggtggca
    29581 gaaaatattt tcctcggcag cgagctttcc cacggcgggc tggtgcagcg aaaagagatg
    29641 ctggtgcagg cgcaaagagt gatcgaccgc ctcggcgcgc agtttaacgc cagcgataag
    29701 gtcatgacgc tgaccattgc cgaacaacag caggtcgaaa tcgcccgcgc gctacatcgc
    29761 aacagccgca ttctggtgat ggacgaaccc accgctgccc tctcctcccg tgaaactcac
    29821 cgtctttttg aactgattat gcggctgcgc gatgaaggga tggcgattat ctacatcagc
    29881 caccgtatgg cggaagtgta tgagctttcc gatcgcgtca gcgtgctgcg cgacgggcaa
    29941 tacgttggca gcctgacccg cgataacctc aatgccgggg agctggtgcg gatgatggtc
    30001 ggcaggccac tgagcgatct gttcaataaa gagcgcgata tcccgctcgg taaagcccgc
    30061 ctgaatgttc accacctgac cgacggcggc aaagtccagc cgagctgcct gcaggtgctt
    30121 tccggcgaaa ttgttggcct cgccgggctg gtgggagccg gacgttccga actggcgcag
    30181 ttaattttcg gcgtgcggaa agcgacaggc ggaatgattg aagtcgatgg cgaaccggtg
    30241 gtgatccact ccccgcgcga agccatcgat cttggcattg gttttctcac cgaaaaccgc
    30301 aaagaacaag gcttattcct tgaaatggcg gcagtcgaaa acatcactat ggcaaccctg
    30361 gagcgcgatg ccagctgggg aatgctcaat cgcaaaaaag cgcaaaccat ttccgatgac
    30421 gccattaagt tgctcaacat tcgcgtgcct catgcccagg tacgcgcggg cgggctttcc
    30481 ggcggcaatc agcaaaaact gttgatctcc cgctgggtgg cgattggccc gcgcatttta
    30541 ctgctcgatg aacccacccg cggcgtggac gttggcgcaa aaagtgagat ctaccggatc
    30601 atgaacgaga tggcgcgcaa gggcgtggcg atcctgatga tctccagcga actgccggag
    30661 atagtgggaa tgagcgatcg cgtccatgtg atgcgcgaag gcagcattgc cggtgagcta
    30721 aatggcaaaa acatcaccca ggaaaacatt atgaccttag cgactggcgt caacgacgcc
    30781 cattcccagg cggtgacctc atgacgacct ctgcaaaaac ctctgccaaa aaaatgctga
    30841 tgagcgatct gatgcaaacg gtcggcattt tgcccatttt gatcctgatc gtagcggtat
    30901 ttggctttat cgcgccaaac ttttttaccg aaagcaacct gctaaatatt acccgccagg
    30961 cgtcgatcaa cattgtgctg gcggcgggga tgaccttcat cattttgacc ggtgggatcg
    31021 atctttctgt aggttcaatc ctcggcacca cggcggtggc ggcaatggtt gtctccctta
    31081 gcccggagtt cgccatgttg tctatcccgg cggcgctgat gctcggcctg ctgctggggc
    31141 tgttcaacgg cgcgctggtg gcctttgccg gattgccgcc gtttatcgtc acgcttggca
    31201 cctatacggc gctgcgcggg gcggcgtatc tgctggctga tggcaccacg gttatcaact
    31261 ccaacattaa tttcgagtgg ataggcaata actatctcgg gccggtcccg tggctggtgg
    31321 tgattgcgct ggcggtgatc gtgctgtgct ggtttattct gcgtcgcacc acgcttggcg
    31381 ttcatatcta cgccgtcggt ggcaatatgc aggcggcgcg tcttaccggc attaaggtct
    31441 ggctggtgct gctgtttgtt tatggcatga gcggcctgct ttcagggctt ggcgggatta
    31501 tgagcacctc gcggctgtac agcgccaacg gcaacttagg tatgggttac gaactggatg
    31561 ccattgcggc ggtgatcctc ggcggcacca gttttgtcgg cggcatcggc accatcaccg
    31621 gcacgctggt gggggcgcta attatcgcca cgctcaacaa tggcatgacc ttaatgggcg
    31681 tctcttactt ctggcagctg gtgatcaaag gggcggtgat catcattgcg gtgcttatcg
    31741 acaaataccg tacccgacat catcaaagtg cataacaaca ataccctaca catgaggaat
    31801 acagcatgag attgaagccc ttagtgaccg cgctctgtgc aggcgcgctg ctggccgcag
    31861 caccgttcgc ccaggcgaaa gatctgaaat cgataggcgt aacggtaggt gacctggcga
    31921 acccgttctt cgtacagatc accaaaggcg cggagctgga agcacgcaaa ctggcgggcg
    31981 ataacgtcaa agtgacgctg gtttccagcg gctacgatct gggtcagcag gtctcgcaga
    32041 tagacaactt tatcgccgct aacgtcgata tgatcatcct taacgctgcc gattcaaaag
    32101 ggatcggccc ggcggtgaaa cgggcaaaag acgccgggat cgtggtggtg gcggttgacg
    32161 tggcggcaga aggtgccgat gccaccatca cttccgataa cacccaagca ggcgaaatgg
    32221 cctgtaaata catcaccgat cgcctgaaag gcaaaggcaa cgtggtgatc atcaacggac
    32281 cgccggtgtc cgccgtgcaa aaccgcgtcg aaggctgcca gaccgaattt aaacgccatc
    32341 ctgacatcaa ggtgctctct gacaacaaga acgccaaagg cagtcgtgag ggcgggctgg
    32401 aggtgatgac cgggctgctg gcggcaaatc cgaagatcga cggcgtattt gcgattaacg
    32461 atccgacagc gatcggtgcc gatctggcag caaaacaggc gcaacgtaac gagttcttta
    32521 ttgtcggcgt ggatggaagc ccggacgccg aagaggcgct caagcgtgag aactcgttgt
    32581 ttgtcgctac tccggcgcaa gatccacagg tgatggcagc gaaagcggtt gagatcagtt
    32641 atgacatttt gcagggcaaa cctgcgccga aagagccggt gctgatcccg gtgacgatga
    32701 tcgataaaaa caacgtcggc aattacaaag gctggacggt gaaataagtc cacacgccgg
    32761 gcggcgatgc tgcccggcca ccgctaagga gaacgctttc gatgaaacgc tccgctataa
    32821 acaacattct cggccataca cggcaattct tttcgcaaca tgacgtacat ctgccgccct
    32881 ttgccagttt ctctcctgct caatggcagc agctcgacac cgccgcgtgg gaggtggttt
    32941 ttgatctcaa actcggctgg gatgttaccg cttttggtgg taacaacttt gcagcacaga
    33001 ggctgacgct gtttaccctg cgtaacggct cagcaaaagg aatgccgtat gtgaaatgtt
    33061 acgccgagaa gatcatgcat gtgcgcgatg cgcaggtcac gccaatgcat tttcactggc
    33121 gtaagcgtga ggacatcatc aatcgcggcg gcggcaattt gattgttgaa ctgtggaatg
    33181 ccgatagcaa cgagcaaaca gttgataccg acgttacggt ggtgatcgac ggctgccgcc
    33241 agactcatgt cgcaggcagt cagttacgcc tgtcccccgg cgaaagtatc tgcctgccgc
    33301 ccggcctgta tcacagcttt tgggcggaaa ccggttttgg cgatgttctg gttggcgaag
    33361 tctcttctgt caatgacgac gaccacgata accacttttt acaaccgctg gaccgctaca
    33421 acctgattga cgaagacgaa ccggcccagc tggtgttgtg taacgaatac cgccagttcc
    33481 tctgaataag gataacgtta tgccgttgat ttctctcgct gaagggctgg cacatgcccg
    33541 cgaacatcac tatgcgttag gtgcgtttaa tgtgctggac tcacactttt tacgcgcctt
    33601 gtttgctgct gccagacaag aacgttcgcc ctttattatc aacattgccg aagtacattt
    33661 taaatatatc tcgctggatt ccctggtcga gacagtgaaa tttgaagcgg cgcgtcaagc
    33721 gattccggtg gtattaaatc tcgatcacgg gctgcatttt gaatcggtgg cgcaagcgtt
    33781 gcggctggga ttcagctcgg taatgtttga tggctccacg ctggggtatg aagagaatat
    33841 ccgccagacg cgggaagtgg tgaaaatgtg ccatgccgtt ggcgtatcgg tagaagctga
    33901 actgggcgcg gtgggtggtg acgaaggcgg tgcgctttac ggtcatgccg atgaatctct
    33961 gtttaccgat ccgcagctgg cgcgtgattt tgtcgaccgt accggcattg acgcgctggc
    34021 cgtggcgatt ggcaatgcgc acggcaaata tcgcggcgaa ccgaaactcg atttcccgcg
    34081 cctcgacgct atccgccagc aggcggctat cccgctggta ttacacggcg gttcagggat
    34141 tagagatgcc gatttccgcc gcgccatcga actgggtatc cacaaaatca atttctacac
    34201 cggcatgtca caagcggcgc tggctgccgt tgaggcccga atggcgcacc gtcagccgat
    34261 gtatgacgag tttgccgagt tactactagc agtggaagcc gccatcagcg atacggttgc
    34321 acaacagatg cgtattttcg gcagtgcggg gcaggcatga tggaacgtaa agggattatc
    34381 gccgcaggca atatgctggt tgaccacgtc caccagatta tccagtggcc agagcgcggc
    34441 tggctggcgg agattaccca cagcgaacgc tccaccggcg gcgcgccgct taacgtcctg
    34501 ctgacactgg cgaaaatgcg caccgggttg ctgttacagg cggtcgggct aatcggtcag
    34561 gacagcgacg gcgattacat tatggcgatg cttgagcaat atcacgtcaa tcgtcagcat
    34621 gtgcagcgca ctacctttgc cccgacgtca atgtcgcagg taatgactga tccaagcggg
    34681 cagcggacgt ttttccattc gccaggtgca aacagacttt tggatcttcc ggcgttcgat
    34741 cagctcgata acaccatgaa gattttccat ctcggctatt tactgttgct cgacagcctg
    34801 gatatgccgg acgatgttta cggcacccgt agcgcacgcc tgctggcgca aatgcgtgat
    34861 actggctttg aaacctcact cgacctggtc tcccgcaagg gcgatccgcg ttatcagccg
    34921 ctggtgttgc ccgcgttgcg ccatctggat tacctggtaa ttaacgaact ggaggccggg
    34981 gagtttagcg ggctggaaat tcggttaccc aacggcgaac cgcatatcgc gcatattgct
    35041 gccgccgcgc gtgaactgtt agatgcgggc gtgcgccagc gtgtggtgat ccactgcccg
    35101 gaaggggcgt ggggagagac cccggaacag tgcggcgtgt ggatcccttc gcaaaagtta
    35161 gaacaacggg aaattatcgg cagcgtcggt gcgggcgatg tattttgtgc cggattttta
    35221 tatggctgcc atgaacgctg gacgcttacc gagagcatca aactggcaca cgcctgcgcg
    35281 cgagcaagcc tgttatgcgc caatgccatc gacggggcga aaacgctgga agagctgcaa
    35341 acagaaatgt cggattaatg cacgtcggca gcgaagacat accccagacc gcgcagagtt
    35401 ttaatcaaca tcggctgatg cgggttgagt tcgattttgc gtcgtaagcg catgattaat
    35461 acgtcgatgg tgcgatcaaa cacctccacg ctgtcgctat gagtgaacgc cagcagctgt
    35521 tcgcggctta atacccgacg ggcattttga gccagggcca gtaacaagcc atattcgccc
    35581 tgagtcagct cgacatgttg ttgtagcgga ctatgcagtt ggcagcgaat ggtatcgaga
    35641 taccagccat tgaatgacag cccggcgttt ttctcctcat taaccgccag cgcgccaacc
    35701 cgtcgcaaca cgctttcagt cgtgcgacca ccacccgtgg ggtgaacggc ttgcagatgt
    35761 aatcatccgc gcccatttcc agcccgacca ccacatcaga ctcactcccc attccggtca
    35821 acatcaccac aggcaatgcc ggacgcagtt tctggatttg ctgcaatacc ctcaggccgt
    35881 tggtatcggg cagcatcata tccagcacca ccagcgcaat gtcaggctcg ccctcaatac
    35941 gcgatatggc ctcctgtccg gtatggctga ctgaaaccgt aaagacatgt tcacttaaca
    36001 cgtcctgcaa cagtgcgcag atagccgtgt catcatccac caccagcacg acgggtttca
    36061 tggttacact ccgcatcatt catctaatta atcattgtgc gtacttcttg tcgaagtgaa
    36121 agcggaaagt aatataaata ataaaaatat aagtcaggtc acattattga tgatagtaat
    36181 aaatatgccg tgcttttccc tcttcagcac aaatgcctaa taaaatggcg cgttccgctc
    36241 acacttccac ttattaaagt cgcaaattcc caattatgat aaccccagct ttaaatgacg
    36301 tattttgcat ggattacata tttacgacac aaatgataaa tggagattta tatgaataac
    36361 tctcggttat tccgtttgag cagaattgtt attgcgttaa ctgccgccag cggcatgatg
    36421 gtaaataccg ctaacgcgaa agaggaagcg aaagccgcca ctcaatatac ccaacaggtt
    36481 aatcagaatt acgccaaatc attaccgttt agcgatcgtc aggattttga cgacgctcag
    36541 cgtggattta tcgctccgct gctggatgaa ggtattctgc gcgatgctaa tggcaaacca
    36601 tattatcg
//
LOCUS       DAJTXY010000023        36520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTXY010000023 DAJTXY010000000
VERSION     DAJTXY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.023"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(156..1391)
                     /locus_tag="OO566_001312"
     CDS             complement(156..1391)
                     /locus_tag="OO566_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6604664.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OO566_001313"
     tRNA            complement(1538..1614)
                     /locus_tag="OO566_001313"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="OO566_001314"
     tRNA            complement(1657..1743)
                     /locus_tag="OO566_001314"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="OO566_001315"
     tRNA            complement(1764..1839)
                     /locus_tag="OO566_001315"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="OO566_001316"
     tRNA            complement(1898..1974)
                     /locus_tag="OO566_001316"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="OO566_001317"
     CDS             complement(2077..3462)
                     /locus_tag="OO566_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6604665.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO566_001318"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO566_001318"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6604666.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO566_001319"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO566_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6604667.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO566_001320"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO566_001320"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6604668.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO566_001321"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO566_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6604669.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRFGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO566_001322"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO566_001322"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6604670.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO566_001323"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO566_001323"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6604671.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO566_001324"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO566_001324"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6604672.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO566_001325"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO566_001325"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6604673.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO566_001326"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO566_001326"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6604674.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO566_001327"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO566_001327"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6604675.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAVNYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO566_001328"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO566_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6604676.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO566_001329"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO566_001329"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6604677.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO566_001330"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO566_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6604678.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO566_001331"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO566_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6604679.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO566_001332"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO566_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6604680.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="OO566_001333"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="OO566_001333"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6604681.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="OO566_001334"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="OO566_001334"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6604682.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO566_001335"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO566_001335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6604683.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAVKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="OO566_001336"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="OO566_001336"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6604684.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO566_001337"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO566_001337"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6604685.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="OO566_001338"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="OO566_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6604686.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO566_001339"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO566_001339"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6604687.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO566_001340"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO566_001340"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6604688.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO566_001341"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO566_001341"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6604689.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO566_001342"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO566_001342"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6604690.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="OO566_001343"
     CDS             complement(31267..32913)
                     /locus_tag="OO566_001343"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6604691.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="OO566_001344"
     CDS             complement(32916..32966)
                     /locus_tag="OO566_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604692.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO566_001345"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO566_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6604693.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="OO566_001346"
     CDS             33502..35022
                     /locus_tag="OO566_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR6604694.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="OO566_001347"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="OO566_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6604695.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="OO566_001348"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="OO566_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6604696.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="OO566_001349"
     tRNA            complement(36439..36514)
                     /locus_tag="OO566_001349"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
BASE COUNT         8920 a         9993 c         9200 g         8407 t
ORIGIN      
        1 gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gccatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttatgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacgcc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccaacccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cacgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcataacggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgccaaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcaac tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag tcaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattca gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaattta ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagatcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctctcga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 gctgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag tcctgtgcaa aaattaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcgttagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttaccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcatgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgggc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatggttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca cgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattct ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtggtgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggagcacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggct attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgct ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggag tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcata gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgttcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgctat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DAJTXY010000024        36497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTXY010000024 DAJTXY010000000
VERSION     DAJTXY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.024"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..170)
                     /gene="ygdR"
                     /locus_tag="OO566_001350"
     CDS             complement(<1..170)
                     /gene="ygdR"
                     /locus_tag="OO566_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6604697.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQG"
     gene            complement(308..1021)
                     /locus_tag="OO566_001351"
     CDS             complement(308..1021)
                     /locus_tag="OO566_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6604698.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            complement(1090..1779)
                     /gene="mutH"
                     /locus_tag="OO566_001352"
     CDS             complement(1090..1779)
                     /gene="mutH"
                     /locus_tag="OO566_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6604699.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            2464..2994
                     /gene="rppH"
                     /locus_tag="OO566_001353"
     CDS             2464..2994
                     /gene="rppH"
                     /locus_tag="OO566_001353"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6604700.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            3007..5253
                     /gene="ptsP"
                     /locus_tag="OO566_001354"
     CDS             3007..5253
                     /gene="ptsP"
                     /locus_tag="OO566_001354"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6604701.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            5404..6279
                     /gene="lgt"
                     /locus_tag="OO566_001355"
     CDS             5404..6279
                     /gene="lgt"
                     /locus_tag="OO566_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6604702.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINSELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            6286..7080
                     /gene="thyA"
                     /locus_tag="OO566_001356"
     CDS             6286..7080
                     /gene="thyA"
                     /locus_tag="OO566_001356"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6604703.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            7264..7734
                     /locus_tag="OO566_001357"
                     /pseudo
     CDS             7264..7734
                     /locus_tag="OO566_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            7725..8288
                     /locus_tag="OO566_001358"
     CDS             7725..8288
                     /locus_tag="OO566_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6604704.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            8285..8692
                     /locus_tag="OO566_001359"
     CDS             8285..8692
                     /locus_tag="OO566_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6604705.1"
                     /translation="MNREKGVSSQALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            8677..9000
                     /locus_tag="OO566_001360"
     CDS             8677..9000
                     /locus_tag="OO566_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6604706.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPLANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            9013..12381
                     /gene="recC"
                     /locus_tag="OO566_001361"
     CDS             9013..12381
                     /gene="recC"
                     /locus_tag="OO566_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6604707.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            12557..15445
                     /gene="ptrA"
                     /locus_tag="OO566_001362"
     CDS             12557..15445
                     /gene="ptrA"
                     /locus_tag="OO566_001362"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6604708.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRMADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            15438..18980
                     /gene="recB"
                     /locus_tag="OO566_001363"
     CDS             15438..18980
                     /gene="recB"
                     /locus_tag="OO566_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6604709.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            18980..20806
                     /gene="recD"
                     /locus_tag="OO566_001364"
     CDS             18980..20806
                     /gene="recD"
                     /locus_tag="OO566_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6604710.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(20868..22199)
                     /gene="argA"
                     /locus_tag="OO566_001365"
     CDS             complement(20868..22199)
                     /gene="argA"
                     /locus_tag="OO566_001365"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6604711.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            22431..23684
                     /gene="amiC"
                     /locus_tag="OO566_001366"
     CDS             22431..23684
                     /gene="amiC"
                     /locus_tag="OO566_001366"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6604712.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(23759..23835)
                     /locus_tag="OO566_001367"
     tRNA            complement(23759..23835)
                     /locus_tag="OO566_001367"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23799..23801),aa:Met,seq:cat)
     gene            24044..25141
                     /gene="mltA"
                     /locus_tag="OO566_001368"
     CDS             24044..25141
                     /gene="mltA"
                     /locus_tag="OO566_001368"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6604713.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            25380..26186
                     /gene="tcdA"
                     /locus_tag="OO566_001369"
     CDS             25380..26186
                     /gene="tcdA"
                     /locus_tag="OO566_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6604714.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(26237..26680)
                     /gene="csdE"
                     /locus_tag="OO566_001370"
     CDS             complement(26237..26680)
                     /gene="csdE"
                     /locus_tag="OO566_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6604715.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(26680..27885)
                     /gene="csdA"
                     /locus_tag="OO566_001371"
     CDS             complement(26680..27885)
                     /gene="csdA"
                     /locus_tag="OO566_001371"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6604716.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            28077..28304
                     /locus_tag="OO566_001372"
     CDS             28077..28304
                     /locus_tag="OO566_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6604717.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            28655..29572
                     /gene="gcvA"
                     /locus_tag="OO566_001373"
     CDS             28655..29572
                     /gene="gcvA"
                     /locus_tag="OO566_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6604718.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            29591..29986
                     /locus_tag="OO566_001374"
     CDS             29591..29986
                     /locus_tag="OO566_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6604719.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            29979..31079
                     /gene="rlmM"
                     /locus_tag="OO566_001375"
     CDS             29979..31079
                     /gene="rlmM"
                     /locus_tag="OO566_001375"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6604720.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(31123..31854)
                     /gene="fucR"
                     /locus_tag="OO566_001376"
     CDS             complement(31123..31854)
                     /gene="fucR"
                     /locus_tag="OO566_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6604721.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSSALFHIRLKSHYAHKADIAREALAWIEERMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(31912..32334)
                     /gene="fucU"
                     /locus_tag="OO566_001377"
     CDS             complement(31912..32334)
                     /gene="fucU"
                     /locus_tag="OO566_001377"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6604722.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(32336..33755)
                     /gene="fucK"
                     /locus_tag="OO566_001378"
                     /pseudo
     CDS             complement(32336..33755)
                     /gene="fucK"
                     /locus_tag="OO566_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(33864..35639)
                     /gene="fucI"
                     /locus_tag="OO566_001379"
     CDS             complement(33864..35639)
                     /gene="fucI"
                     /locus_tag="OO566_001379"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6604723.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(35672..>36497)
                     /gene="fucP"
                     /locus_tag="OO566_001380"
     CDS             complement(35672..>36497)
                     /gene="fucP"
                     /locus_tag="OO566_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6604724.1"
                     /translation="GAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSV
                     QTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLA
                     QFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAP
                     HKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKY
                     GSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         8683 a         9104 c         9894 g         8816 t
ORIGIN      
        1 ttaccttgct gatcgtgata actcaccagc ccggtatcat catcaatttc aggttttcca
       61 tcggtcaaaa tcatacggcc atctttggtc gccatgacgt aatcactgga acacccggaa
      121 acagcaaacg ccagtccgac tgcagaaatt attactgccc attttttcat ccggttatcc
      181 tcatttggcc tacctatgat aatagtctgt ttataaatcg acgttatata tcttgaaagc
      241 aggaaaatct taaactgtga gggtaaaagg agtgagtacg ccctcccgca ggagagcgta
      301 cggagtatca gagcggattc tttttgttgc gaatcaagtt gaggctttca accgcaatag
      361 agaagaacat cgcgaagtag atgtaacctt tcggtacgtg gatgtcgaaa ctttccagaa
      421 tcagggtaaa gcccaccaga atcaggaaag agagcgccag catttttacc gaaggatggc
      481 gttcgacaaa atcaccaatc gagcgcgcgg cgaacatcat cacgcctacg gcaatcacca
      541 cggctgccat cataataaac aggtgatctg acagacccac agcggtaatc accgagtcga
      601 ggctgaagat aatatccagc agcataatct gcacgatagc gccgaggaat gatgaaacgc
      661 gtgttttcag cccttcttct tcaccttcaa tggattcgtg gatttccttg ctggctttcc
      721 agataaggaa caagccaccc agaagcagaa tcaaatcacg ggcagaaatt tcctgactga
      781 atattgtaaa aagcggattt gtcaggcgcg taacccaaga gattgatgcc agcagagcca
      841 gacgcataac catggctccc gccaacccca gacggcgcgc atgagcacgt tgtgctgtgg
      901 gaagctttgc caccaccaga gaaaggaaaa taatattgtc gatcccaaga acgatctcca
      961 gcagcgtcag cgtaccgagc gcaagccagg cgttaggatc ggttatccat gcaaataaca
     1021 tctaaaaagc cctgccaaaa aaatgaagcg gtaattatat gcccggaaag cggcaggtca
     1081 aagcgatggc tactggatca gaaaatgacg ggccagtagt gcactggtga aattcttctt
     1141 caaataaaag ccgcgtggca gcgtcagaat ccgttcgccc cgggcaccaa tggcttcggt
     1201 aagcgctttc gcattcgctg ctttcggtct tatctgtaaa tattccccgt gacgagcggt
     1261 gatccgctca acctgaccga gaacaatcat atccattaat tcttcccagt cttcgcgtag
     1321 ctgtcggtct tcctcttcat tcgggctcca cagcaacggt gatcctacgc gacgctgcgc
     1381 cagcgggatg ctgcgctcgc cttcaaccgg tatccacagt acgcgtttga gcttgtggcg
     1441 cacatggctg gtttcccagg tcaccccgct attacccgtt aacggggcaa cacaaacgaa
     1501 tgttgtttcc agcggacgac caagactatc cacagggata gttttaagtt ccacgcccag
     1561 agcagcaaaa tcttgctcag gtttactccc tgcgctggca cctagccaga tctccagtaa
     1621 cacgccaatc cagcccttat cgcgttttaa attctctggc gtaaccagcc cggcaagtgc
     1681 cgccagttct cccaatgtat aaccagaaag ttgctgtgct tgcgctaaca actgttcttc
     1741 agtttcggga ggagagagca gtgggcgagg ttgggacatg tcatgatgcc ttggttaaaa
     1801 aacaaacagc aattatgtgc cgaatattcg cagtgtaact ttttccacat gtgttgttca
     1861 attctctgat ttatattgct ttttaggctg ccggtaatcg agttgcgcaa cactgcaata
     1921 ggttttccaa atctggtcac tgaaaataaa caggatctta caccatgtta tccacagaaa
     1981 gctgggataa ctgtgaaaaa cctccactac tgttttcatt tacagccttg acgtgcgacg
     2041 aaactccagt ttttagtggt tcagtgctta acttagtggc acattctgtg gataaatacg
     2101 gcattggttg atctttcgcc aacccgtaaa tgagaggtgt gacctgggtc acgaattact
     2161 ctttttcatt actcaaatta catgtaatgc attgactaat aatgaatatt ttaaaatgtc
     2221 tatatcgggc ttattcagaa tgctccgggt tttcccgtag taattccgta gttcttcaca
     2281 actctatcca cagaaaaggt gaataaaaac ggctatccac cccttcctct gtttataact
     2341 ctgcacataa ctgtgagtta ttcaattgtt attagtgtgt aatgccctgc agagagtggt
     2401 ttaccgtctt cctggagtat gaaacaatca ttcgtatata aagctttatt ttgaggtagt
     2461 ccggtgattg atgacgatgg ctaccgccca aacgtaggta tcgtgatttg taatcgccag
     2521 gggcaggtaa tgtgggcccg gcgatttggt cagcactcct ggcaatttcc gcaaggcgga
     2581 atcaaccccg gagaatccgc agagcaggcg atgtaccgtg aattgtttga agaagtagga
     2641 ttaagccgca aagacgttcg aatccttgct tcaacgcgta actggttgcg ctacaaatta
     2701 ccgaaacgtt tggtgcgttg ggacacgaag ccggtttgta tcggccaaaa acaaaaatgg
     2761 tttctcttgc agctggtaag cggcgatgca gaaatcaata tgcaaaccag cagtacacca
     2821 gagtttgacg gctggcgatg ggtaagttac tggtatccgg tcagacaggt ggtgtcattt
     2881 aaacgtgatg tctaccgtag ggtaatgaaa gagttcgcga gtgtggtgat gtcactgcag
     2941 gaaaatacgc caaaaccaca aaacgcatct gcttatcgac gtaaaagagg ttaagtcacg
     3001 ccaattatgc tcactcgcct gcgcgaaata gtcgaaaagg tagccagcgc accacgcctg
     3061 aatgaggcgt taaatattct ggttaccgac atctgtcttg cgatggatac cgaggtctgt
     3121 tcggtctacc tggccgatca tgatcgacgt tgttactacc tgatggcgac ccgggggctg
     3181 aaaaaaccac gcggtcgcac tgtaacgctc gcgtttgatg aagggatcgt cggcctggtt
     3241 ggcaggctgg cggaaccgat aaaccttgca gatgcgcaaa agcaccccag cttcaaatac
     3301 atcccctccg taaaagaaga acgtttccgc gcgtttttag gcgtaccaat tattcaacgt
     3361 cgccagttgc ttggtgtact ggtggtacag caacgagagt tgcgccagta tgacgaaagt
     3421 gaagaatcct tcctggtgac gcttgccacc cagatggcag ctattctttc tcagtcgcag
     3481 ttgactgccc tgtttgggca atatcgccag acgcgaatcc gcgcattacc ggcagcacct
     3541 ggtgtggcga ttgccgaagg ctggcaggat gccacgttac ctttaatgga acaggtgtat
     3601 caggcatcaa cgctggatcc ggctctggaa cgcgaacgac tgaccggggc gctggaagaa
     3661 gcggcaaacg agtttcgccg ctacagcaaa cgctttgccg ccggagcaca aaaagaaacg
     3721 gcggctattt tcgatcttta ctcgcacctg ctttcggaca cccggctgcg tcgcgaattg
     3781 tttgccgagg ttgataaagg ctcggtggca gagtgggcgg taaaaacggt cattgaaaaa
     3841 tttgccgaac agtttgccgc gctaagcgat aactatctca aagagcgggc tggcgattta
     3901 cgtgcgctgg gtcagcgatt gctgtttcat cttgatgacg ctaatcaagg gccgaacgcc
     3961 tggccggaac gtttcattct ggtggcagat gaactgtcag cgacaactct tgctgagctg
     4021 ccccaggatc gcttagtcgg tgttgtcgtg cgagatggcg cagccaactc ccatgctgcg
     4081 atcatggtac gtgcgctggg gatccctacc gtgatgggcg cggatattca gccttcggtg
     4141 ctgcatcgta ggacgctgat cgttgatggc tatcgcggtg aattgctggt cgatccggag
     4201 ccggtactgc tgcaagaata tcagcggcta attagtgaag agattgagct tagccgtctg
     4261 gcggaagatg acgtcaattt acccgcgcag ttaaaaagcg gtgagcgtat aaaagtcatg
     4321 ctcaatgctg gtttaagccc ggaacatgaa gaaaaactgg gcagccgtat tgatggcatc
     4381 ggtctttatc gcactgaaat cccattcatg ctgcaaagtg gtttcccgtc ggaagaagaa
     4441 caggtggcgc agtatcaggg gatgctgcaa atgttcaatg ataaacccgt caccttgcgt
     4501 acgctggatg tcggagcaga taagcagctg ccttacatgc cgatcagcga agagaatcca
     4561 tgcctgggtt ggcgtggtat tcgcattacg ctcgatcagc cggagatctt cttgatccag
     4621 gtgcgggcga tgctgcgtgc taatgccgct acgggcaacc tgaatattct gttgccgatg
     4681 gtcaccagcc tcgatgaagt tgacgaagca cgccgcctga ttgaacgtgc cggacgtgaa
     4741 gtcgaggaga tgatcggtta cgaaattccc aaaccacgta tcggcatcat gctggaagtg
     4801 ccgtcaatgg tatttatgct gccgcatctg gcaaagcggg tcgatttcat ctctgttggc
     4861 accaacgatc tgactcaata cattctggcc gttgatcgca acaatacccg ggtggctaac
     4921 atttacgaca gtcttcatcc tgcaatgtta cgagctctgg cgatgatcgc ccgggaagcg
     4981 gaaatacatg gtatcgatct ccgtttgtgc ggtgaaatgg cgggcgatcc catgtgtgtg
     5041 gcaatcctca ttgggcttgg gtatcgccat ctgtctatga acggacgttc tgtagcgcgg
     5101 gtaaaatacc tgctgcggcg cattgatttt gccgaagcag aaaatcttgc gcagcgtagt
     5161 ctggaagcgc aactggcgac cgaagttcgt catcaggttg cagcctttat ggagcgtcgc
     5221 ggcatgggcg ggctgattcg cggagggtta tagcgcggat catatacata tcttttaacg
     5281 gtatccggca accagccagg tccccttgtg ctattattcg cacctttgga gcgcctgaaa
     5341 cctgcggcgc gcatttcaat cgctgttctc tttcagcgaa ataacaagaa cttgtggtga
     5401 cagatgacca gtagctatct gcattttccg gagtttgatc cggtcatttt ctcaatagga
     5461 cccgtggcgc ttcactggta cggcctgatg tatctggtgg gtttcatttt tgcaatgtgg
     5521 ctggcaacac gtcgggcgaa tcgtccgggc agtggctgga ccaaaaatga agttgaaaac
     5581 ttactctatg cgggcttcct cggcgtcttc ctcgggggac gtattggtta tgttctgttc
     5641 tacaatttcc cgcagtttat ggccgatccg ctgtatctgt tccgtgtctg ggacggcggc
     5701 atgtctttcc acggcggcct gattggcgtt atcgtggtga tgattatctt cgcccgccgt
     5761 actaaacgtt ccttcttcca ggtctctgat tttatcgcac cactcattcc gtttggtctt
     5821 ggtgccgggc gtctgggcaa ctttattaac agtgaattgt ggggccgcgt tgacccgaac
     5881 ttcccgtttg ccatgctgtt cccaggctcc cgtacagaag atattttgct gctgcaaacc
     5941 aacccgcagt ggcaatccat tttcgacact tacggtgtgc tgccgcgcca cccatcacag
     6001 ctttacgaac tgctgctgga aggtgtggtg ctgtttatta tcctcaacct gtatattcgt
     6061 aaaccgcgcc caatgggagc tgtctcaggc ttgttcctga ttggctacgg cgcgtttcgc
     6121 atcattgttg agtttttccg ccagcccgac gcgcagttta ccggtgcctg ggtgcagtac
     6181 atcagcatgg ggcaaattct ttccatcccg atgattgtcg cgggtgtgat catgatggtc
     6241 tgggcatatc gtcgcagccc acagcaacac gtttcctgag gaaccatgaa acagtattta
     6301 gaactgatgc aaaaagtgct cgacgaaggc acacagaaaa acgaccgtac cggaaccgga
     6361 acgctttcca tttttggtca tcagatgcgt tttaacctgc aggatggatt cccgctggtg
     6421 acaaccaaac gttgccacct gcgttccatc atccacgaac tgctgtggtt cctgcagggc
     6481 gatactaaca ttgcttatct acacgaaaac aatgtcacca tctgggacga atgggccgat
     6541 gaaaatggcg acctcgggcc agtgtatggt aaacagtggc gcgcctggcc aacgccagat
     6601 ggtcgtcata ttgaccagat tactacggta ctgaaccagc tgaaaaacga cccggattcg
     6661 cgccgcatta ttgtttcagc gtggaacgta ggcgaactgg ataaaatggc gctggcaccg
     6721 tgccatgcat tcttccagtt ctatgtggca gacggcaaac tctcttgcca gctttatcag
     6781 cgctcctgtg acgtcttcct cggcctgccg ttcaacattg ccagctacgc gttactggtg
     6841 catatgatgg cgcagcagtg cgatctggaa gtgggtgatt ttgtctggac cggtggcgat
     6901 acgcacctgt acagcaacca tatggatcaa actcatctgc aattaagccg cgaaccgcgt
     6961 ccgctgccga agttgattat caaacgtaaa cccgaatcca tcttcgacta ccgtttcgaa
     7021 gactttgaga ttgaaggcta cgatccacat ccgggcatta aagcgccggt ggctatctaa
     7081 ttacgaaaca tactgccaga gccgacgcca gtgtgcgtcg gtttttttac cctccgttaa
     7141 attcttcgag acgccttccc gaaattttgc aacgtcctgc aacggcgtaa atagtccgga
     7201 agatgcgccg aagaaataga aactggccct cgtctttttt tctcctcgct ccatactgcc
     7261 ggcatgaaaa cacaacgtgg ttatacgctg attgaaacgc tggtcgcgat gctgattttg
     7321 gtcatgctaa gcgcaagtgg actctatggc tggcaatact ggcagcagtc gcaacggctt
     7381 tggcaaaccg ccagctaggc gcgggactat ttgctctatt tacgtgaaga tgccaactgg
     7441 cataaccgcg accacagtat cagcgttatc agggagggga cgttatggtg ccttgtgagt
     7501 tccgctgctg gggccaatac ctgtcatggc agttcaccat tggtctttgt gccacgctgg
     7561 cccgaagtcg aaatgagcga cctgacacct tcgcttgctt tctttggcct gcgcaatacc
     7621 gcatgggccg ggcatattcg cttcaaaaac tcaacgggcg agtggtggct ggtggtttcg
     7681 ccgtggggaa gactccggct ttatcagcaa ggagaaacag aaggatgcct gtaaaagagc
     7741 aaggtttttc tctgctggaa gtgttgattg ctatggcgat cagtagcgta ttgttgctgg
     7801 gggctgcacg ctttctgcct gcgttacagc gtgaaagttt aacgagcact cgtaagctgg
     7861 cactggaaga tgaaatctgg ctgcgggtat ttaccgtcgc gaagcatctc cagagggcgg
     7921 gttattgtca tggcagctgt accggcgaag ggctggaaat tgtcggacag ggtgactgta
     7981 tcattgtgca gtgggatgcg aacagtaacg gtatctggga tcgcgaaccg gtaaaagagt
     8041 ccgaccagat tggatttcgt ctgaaggagc atgtgctgga aacgctacgc ggtgcgacat
     8101 cctgtgaagg taagggctgg gataaagtca ctaatccgga tgccatcatt atcgacactt
     8161 ttcaggtcgt acgtcaggat gtcagcggct tctcgccggt gttgacggtt aatatgcgtg
     8221 ctgccagtaa gtctgaaccg caaaccgtgg tggatgccag ctatagcgtg acaggattca
     8281 acctgtgaac cgcgaaaaag gtgtttcgtc acaggctctg gtcctgatgc tgctggtttt
     8341 gggtagcttg ctattgcaag gaatgagtca gcaggatcgc agttttgcct ctcgcgtgag
     8401 catggaaagt cagtcattgc gccgccaggc catcgttcag tcggcgctgg cgtggggaaa
     8461 aatgcactcc tggcagacgc agccagcagt tcagtgctcg cagtacgctg gaaccgatgc
     8521 ccaggtttgt ttgcgtttac tggcagataa tgaagcctta ttgattgccg gttatgaagg
     8581 cgtttcgttg tggcgaacag gcgaagtcat tgatggaaaa attgtttttt cgccacgcgg
     8641 ctggagcgat ttttgtccgc tgaaagaggg ggcattatgt cagcttccct gaagaatcaa
     8701 caaggcttta gcctgccgga ggtaatggtt gcgatggtgc tgatggtgat gattgtcact
     8761 gcgttatcgg gtatccagcg aacattaatg aacagtctcg ccagcagaaa ccagtaccaa
     8821 cagctctggc ggcatggttg gcagcaaacg caactgcgcg cgatttcgcc acttgccaac
     8881 tggcaggtca accgaatgca gacatcgcag gcgggatgtg tcagcatcag cgttacgcta
     8941 gtttcacccg ggggcagaga aggcgagatg acccgcctgc attgcccgaa tcgtcagtag
     9001 tcaggagccg ctatgttaag ggtctaccat tccaatcgtc tggacgtgct ggaagcgttg
     9061 atggagttta ttgtcgaacg cgaacggctg gacgatcctt tcgaaccaga gatgattctg
     9121 gtgcaaagta ccggtatggc gcagtggctg caaatgaccc tgtcgcaaaa gtttggtatt
     9181 gcggcaaaca ttgattttcc gctgccagcg agctttatct gggatatgtt cgtccgggtg
     9241 ttaccggaaa tccccaaaga gagcgccttt aacaaacaga gcatgagctg gaaactgatg
     9301 actctgctgc cgcaattgct ggagcgcgaa gactttaccc tgttgcggca ttatctgact
     9361 gacgatagcg acaagcgaaa actgttccag ctttcctcaa aagcggcgga cctgtttgac
     9421 cagtatctgg tctatcgtcc ggactggctg gcacagtggg aaacaggaca tttggttgaa
     9481 gggctgggag aagcacaggc ctggcaagcc ccgttgtgga aggcgctggt ggaatatacc
     9541 catgaactcg gacaaccgcg ctggcaccgc gcgaatctct atcagcgctt tatcgaaacg
     9601 ctggagtccg cgacgacctg cccgccgggg ttaccttcgc gcgtctttat atgcggtatt
     9661 tccgcgttac cgcctgttta tctccaggcg ctccaggcgc tgggtaaaca tattgaaatc
     9721 catctcctgt ttaccaaccc ctgccgttat tactggggcg atattaaaga tcctgcttat
     9781 ctggcgaaac tgctgactcg tcagcgtcga cacagttttg aagatcgcga attgccgcta
     9841 tttcgcgata gcgaaaatgc cggacagctc tttaacagcg atggtgaaca ggatgtcggc
     9901 aacccgctgc tggcctcatg ggggaagctt gggcgcgact acatttatct cctttctgac
     9961 ctggagagca gccaggagct ggacgctttt gtcgatgtga cgccagataa cctgctgcat
    10021 aacattcagt ctgacattct ggaactggaa aaccgcgccg ttgcgggtgt gaacatcgaa
    10081 gagttttccc gtagcgataa caaacgcccg cttgatccac tggatagcag tatcacattc
    10141 cacgtttgcc atagcccgca gcgtgaagtt gaagttttac acgatcgctt gctggcgatg
    10201 ctggaggaag ccccgacact tactccgcgc gacatcatcg tgatggtggc tgatatcgac
    10261 agctacagtc cgtttattca ggcagtgttt ggtagcgcac ctgcggatcg ttacctgcct
    10321 tacgccattt ccgaccgtcg tgcgcggcag tcacatccgg tactggaagc gtttatcagc
    10381 ctgttatcac tgcctgacag tcgctttgtg tcagaggatg tgctggcgct gctggatgtg
    10441 ccggtgctgg cggcgcggtt tgacatcacc gaagaagggc tgcgttattt acgccagtgg
    10501 gtcaacgaat ccggcattcg ttgggggata gatgacgaca acgttcgcga gctggaactc
    10561 cccgccaccg gacaacacac ctggcgattt ggcctgacgc gtatgttgct gggctacgcg
    10621 atggagagcg cgcagggcga gtggcaatcg gttctacctt atgatgaatc gagcggctta
    10681 attgcagaac tggtggggca tctggcttca ctgctaatgc agctaaacat ctggcgtcgc
    10741 gggctggcac aggagcgtcc gctagaagag tggttgccgg tttgtcgcga tatgctcaac
    10801 gccttcttcc tgccggatgc ggaaaccgaa gcggcgatga cgctgatcga acaacaatgg
    10861 caggcgatta tcgccgaagg tttaggtgcg cagtatggcg acgcggtgcc gctgtcacta
    10921 ttgcgtgatg aactggcaca gcgtctggat caagaacgta tcagccagcg ttttctcgcc
    10981 ggaccggtta atatttgtac tctgatgcca atgcgttcaa ttccgttcaa agtggtttgc
    11041 ctgctgggaa tgaacgacgg cgtttatcca cgtcagcttg cgccattggg ctttgacctg
    11101 atgagccaga aaccgaagcg tggcgaccgt agccgtcgcg atgacgaccg ctacctgttc
    11161 ctggaagcgt taatttccgc gcagcaaaaa ctctatatca gctatatcgg gcgttccatt
    11221 caggataaca gtgaacgttt cccgtcggta ctggtgcagg aactgatcga ctacatcggg
    11281 caaagtcatt atctaccggg cgatgaagcg ctcaactgtg atgaaagcga ggcaagggta
    11341 aaagcgcatc ttacttgcct ccatacccgg atgccgtttg atccgcaaaa ctaccagcca
    11401 ggcgaacgac aaagctatgc tcgtgaatgg ctacctgcgg ccagccaggc tggtaaagca
    11461 cattctgaat ttgttcagcc gctgccgttt accttaccgg aaaccgtgcc gctggaaacg
    11521 ctacaacgat tctgggcaca tccggtgcgg gcgttcttcc agatgcgttt gcaggtgaac
    11581 ttccgtactg aagacagcga aatcccagac accgagccat ttattctgga aggacttagc
    11641 cgttatcaaa tcaatcagca gttattgaat gcactggttg agcaggatga tgccgaacgc
    11701 ttgttccgcc gcttccgggc ggcaggggat ttaccgtatg gcgcttttgg tgaaattttc
    11761 tgggaaacac agtgccagga gatgcagcag cttgccgaca gagtcattgc ctgtcgccag
    11821 ccggggcaga gtatggaaat tgatctcgcc tgcaacggtg tgcagataac tggctggttg
    11881 ccgcaggtgc agccggatgg cctgttgcgc tggcgtccct ctttattaag tgtggcgcag
    11941 ggaatgcaac tttggctgga acaccttgtc tactgtgcca gcggtggtaa tggcgaaagt
    12001 cgcctttttc tacgcaaaga cggtgagtgg cgttttccgc cgcttgcagc cgaacaggct
    12061 ttgcattacc tctcacaact gattgagggg tatcgtgaag gaatgtccgc gccattgctg
    12121 gtgttacctg aaagtggcgg cgcgtggcta aaaacctgtt atgacgcgca aaacgatgcc
    12181 atgctggatg acgattctac gttgcaaaaa gcccgtacga aattccttca ggcttacgaa
    12241 ggcaacatga tggtgcgtgg cgaaggtgat gatatctggt atcaacggct ctggcggcaa
    12301 ttaacaccag agacaatgga ggccatcgtt gaacagtcgc aacgtttcct gttaccgctg
    12361 tttcgcttta atcagtcatg agtgttgtat aaaaattgcg caatctatcc gcttacttta
    12421 tgatgcgcac cagtcacgga ctgatggtta tataaacata ggctgactcg tgcagcacaa
    12481 gattaaattc tggcagatga tttgcgttaa cctgttgaat ctgtccagaa aataaagttg
    12541 attatgaggt ccgtgaatgc cccgcagcac ctggttcaaa gcattattgt tgtttgttgc
    12601 cctctgggca ccattaagtc aggcagaaac gggatggcag ccgattcagg aaaccatccg
    12661 taaaagtgat aaagataacc gccagtatca ggctatacgt ctggataacg gtatggtggt
    12721 cttgctggtt tctgatccgc aggcagttaa atcgctctcg gcgctggtag tgcccgttgg
    12781 gtcgctggaa gatcccgagg cgtaccaggg gctggcacat taccttgaac atatgagtct
    12841 gatggggtcg aaaaagtacc cgcaggctga cagtctggcc gaatatctca aaatgcacgg
    12901 cggtagtcac aatgccagca ctgcgccgta tcgcacggct ttctatctgg aagttgagaa
    12961 cgacgccttg cccggtgcgg tagaccgcat ggccgatgct attgcagaac ccttgctcga
    13021 caagaaatac gccgaacgtg agcgtaatgc agtgaacgct gaattaacca tggcgcgtac
    13081 gcgtgacggg atgcgcatgg cacaggtcag cgcagaaacc attaacccgg cacaccccgg
    13141 ttcaaagttt tctggtggta acctcgaaac tttaagcgac aaacctggta atccggtgca
    13201 gcaggcgctg aaagatttcc acgagaagta ctattccgcc aatttgatga aggcggttat
    13261 ttacagtaat aaaccgctgc cggagttggc aaaaatggcg gcggacacct ttggtcgcgt
    13321 gccgaacaaa gagagcaaaa aaacggaaat caccgtgccg gtagtcaccg acgcgcaaaa
    13381 gggcattatc attcattacg tccctgcgct gccgcgtaaa gtgttgcgcg ttgagtttcg
    13441 catcgataac aactcagcga agttccgtag taaaaccgat gaattgatta cctatctgat
    13501 tggcaatcgc agcccaggta cactttctga ctggctgcaa aagcagggat tagttgaggg
    13561 cattagcgcc aactccgatc ctatcgtcaa cggcaacagc ggcgtattag cgatctctgc
    13621 gtctttaact gataaaggcc tggctaatcg cgatcaggtt gtggcggcaa tttttagcta
    13681 tctcaatctg ttacgtgaaa aaggcattga taaacaatac ttcgatgaac tggcgaatgt
    13741 gctggatatc gacttccgtt atccgtcgat cacccgtgat atggattacg tcgaatggct
    13801 ggcagatacc atgattcgcg ttcctgttga gcatacgctg gatgcagtca atattgccga
    13861 tcggtacgat gctaaagcag taaaggaacg tctggcgatg atgacgccgc agaatgcgcg
    13921 tatctggtat atcagcccga aagagccgca caacaaaacg gcttactttg tcgatgcgcc
    13981 gtatcaggtc gataaaatca gcgcacaaac tttcgccgac tggcagaaaa aagccgccga
    14041 cattgcgctc tctttgccag agcttaaccc ttatattcct gatgatttct cgttgattaa
    14101 gtcagagaag aaatacgacc atccagagct gattgttgat gagtcgaatc tgcgcgtggt
    14161 gtatgcgcca agccgttatt ttgccagcga gcccaaagct gatgtcagcc tgattttgcg
    14221 taatccgaaa gccatggaca gcgcccgcaa tcaggtgatg tttgcgctca atgattatct
    14281 cgcagggctg gcgcttgatc agttaagcaa ccaggcgtcg gttggtggca taagtttttc
    14341 caccaacgct aacaacggcc ttatggttaa tgctaatggt tacacccagc gtctgccgca
    14401 gctgttccag gcattgctcg aggggtactt tagctatacc gctacggaag agcagcttga
    14461 gcaggcgaag tcctggtata accagatgat ggattccgca gaaaagggta aagcgtttga
    14521 gcaggcgatt atgcccgcgc agatgctctc gcaagtgccg tacttctcgc gagatgaacg
    14581 gcgtaaaatt ttgccctcca ttacgttgaa agaggtgctg gcctatcgcg acgccttaaa
    14641 atcaggggct cgaccagagt ttatggttat cggcaacatg accgaggccc aggcaacaac
    14701 gctggcacgc gatgtgcaaa aacagttggg cgctgatggt tcagagtggt gtcgaaacaa
    14761 agatgtagtg gtcgataaaa aacaatccgt catctttgaa aaagccggta acagcaccga
    14821 ctccgcactg gcagcggtat ttgtaccgac tggctacgat gaatacacca gctcagccta
    14881 tagctctctg ttggggcaga tcgtacagcc gtggttctac aatcagttgc gtaccgaaga
    14941 acaattgggc tatgccgtgt ttgcgtttcc aatgagcgtg gggcgtcagt ggggcatggg
    15001 cttccttttg caaagcaatg ataaacagcc ttcattcttg tgggagcgtt acaaggcgtt
    15061 tttcccaacc gcagaggcaa aattgcgagc gatgaagcca gatgagtttg cgcaaatcca
    15121 gcaggcggta attacccaga tgctgcaggc accgcaaacg ctcggcgaag aagcatcgaa
    15181 gttaagtaaa gatttcgatc gcggcaatat gcgcttcgat tcgcgtgata aaatcgtggc
    15241 ccagataaaa ctgctgacgc cgcaaaaact tgctgatttc ttccatcagg cggtggtcga
    15301 gccgcaaggc atggctattc tgtcgcagat ttccggcagc cagaacggga aagccgaata
    15361 tgtgcatcct gaaggctgga aagtgtggga gaacgtcagc gcgttgcagc aaacaatgcc
    15421 cctgatgagt gaaaagaatg agtgatgtcg ccgagacact agatcctttg cgcttgccct
    15481 tacagggcga gcgcctgatt gaagcctctg ccggcacagg caaaaccttt acgattgcgg
    15541 cgctctattt gcgcctgtta cttggactag gcggttccgc cgcctttccc cgcccgctga
    15601 tcgttgaaga actgctggtg gtcaccttta ccgaggctgc cacggcagaa ttgcgcggtc
    15661 gtatccgtag caatatccac gagttgcgca tcgcctgtct gcgtgaaacc accgacaatc
    15721 cactgtacga acgcctgctg gaagagatcg acgataaagc gcaagccgcg cagtggttgt
    15781 tgttagccga acggcagatg gatgaagcgg cagtctttac tattcacggc ttttgccagc
    15841 gcatgctcaa cctgaatgcc tttgaatccg gcatgctgtt tgagcagcag ctgattgaag
    15901 atgagtctct gctacgctac caggcctgcg ccgatttctg gcgtcgccac tgctacccgc
    15961 tgccgcgtga aatagcccag gtcgtctttg aaacctggaa agggccgcag gcgttgctgc
    16021 gcgatattaa tcgttatctg caaggcgaag cgccggttat caaagcaccg ccgcccgatg
    16081 atgaaacgct ggcttcccgc cacgcgcaaa ttgtggcgcg tattgatacg gtaaaacagc
    16141 agtggcgcga cgcagtgggt gaactggatg cgctgatcga atcttctggt attgatcgac
    16201 gcaagtttaa ccgtagcaat caggctaaat ggatcgaaaa gatcagcgct tgggcagaag
    16261 aagagacaaa cagttatcag ttgccggagt cgctggaaaa attctcccag cgtttcttag
    16321 aagatcgcac gaaggccggg ggggaaaccc cgcgacatcc actgtttgag gtgatcgatc
    16381 aactgcttgc agaaccattg tcgatccgcg atctggtgat cacccgagca ttggctgaga
    16441 tccgcgaaac agtagcgcgt gaaaaacgcc gccgtggcga attgggtttt gatgacatgt
    16501 taagtcggct cgattccgcg ctgcgtagcg aaagcggcga ggtgttggca gcggcgatcc
    16561 gtacgcgatt cccggtggca atgatcgatg aatttcagga taccgacccc cagcagtacc
    16621 gaatttttcg ccgtatctgg caccatcagc cggaaaccgc attgttgctg attggcgacc
    16681 cgaagcaggc catatatgca ttccggggtg cggatatctt cacttatatg aaggcgcgta
    16741 gtgaagttca cgcccactac actttagaca ccaactggcg ctccgcacct ggaatggtga
    16801 acagtgtgaa caagcttttc agccagactg atgacgcgtt catgtttcgc gaaataccgt
    16861 ttattccagt gaaatcagcc gggaaaaatc aggcgttacg ttttgtattt aaaggtgaaa
    16921 cgcagcctgc gatgaaaatg tggctgatgg aaggcgaaag ctgcggcgtt ggcgactatc
    16981 aaagtaccat ggcgcaggta tgtgctgcgc aaatccgcga ctggctacaa gccggacagc
    17041 ggggcgaagc gttgctgatg aacgacgacg acgcgcgtcc ggtgcgtgct tcggacatca
    17101 gcgtgctggt gcgcagccgc caggaggccg cccaggtgcg cgatgcctta acgttgctgg
    17161 aaatcccttc cgtttacctt tcgaaccgcg acagtgtttt tgaaactctg gaagcgcagg
    17221 aaatgctttg gttgttgcag gcggtgatga cgcccgaacg tgagaacacc ctgcgcagtg
    17281 cgctggcaac gtcaatgatg gggctgaatg cgctggatat tgaaacgctg aataatgacg
    17341 aacatgcgtg ggatgcggta gtcgaagagt tcgatggtta tcggcaaatc tggcgcaaac
    17401 gtggcgttat gccgatgctg cgggcgctga tgtcggcgcg taacattgca gaaaacttgc
    17461 tggcaacggc aggcggtgag cggcgtctta ccgatatctt gcatatcagc gaactgcttc
    17521 aagaagccgg aacgcagctg gaaagtgaac atgcgctggt acgctggtta tcgcaacata
    17581 tcctcgagcc agacagtaat gcctccagcc aacaaatgcg cctcgaaagt gataaacatc
    17641 tggtgcagat tgtcacgatc cataaatcga aagggctgga atatccgctg gtctggttgc
    17701 catttattac caatttccgc gtccaggatc aggcgtttta tcacgatcgc cactcgtttg
    17761 aggcagtttt ggatcttaat gctgcgccag aaagcgtcga cctcgcggag gtcgaacgtc
    17821 tggcggaaga tctgcgtttg ctttacgtgg cgctgacgcg ttcggtttgg cattgcagtc
    17881 tcggcgttgc accgctggtg cgccgtcgtg gcgataaaaa aggtgacacc gacgtccacc
    17941 aaagtgcgct cgggcgtttg ctgcaaaaag gggaaccgca agatgcggca gggcttcgca
    18001 cctgtattga agcgttatgc gatgatgata ttgcctggca aacggcacaa actggtgata
    18061 accagccctg gcaggttaat gatgctttaa ctgcagaact gaatgcgagg acactacaac
    18121 gattgcccgg cgataactgg cgtgtcacca gctactccgg cttgcagcag cgtggtcacg
    18181 gtatcgctca ggatctgatg ccacggctgg atgttgatgc cgcaggcgtg gtcagcgtcg
    18241 ttgaagaacc gacgttaaca ccgcatcagt tcccgcgcgg tgcgtcaccg gggacattct
    18301 tgcacagttt gtttgaagac ctcgatttta cccagccggt tgacccgaac tgggtacagg
    18361 aaaaactgga actcggcggc tttgaatcgc agtgggaacc ggtgttgacc gagtggatca
    18421 cggctgtcct ccaggcacct ctcaatgaaa cgggtgttag cctgagtcag ctttccgacc
    18481 gcgataaaca ggtggagatg gagttttacc tgccgattag cgaaccgctc atcgccagcc
    18541 agcttgatgc actaattcgc cagtttgacc cgctatccgc tggctgcccg ccgctggagt
    18601 tcatgcaggt acgcggcatg ttaaaaggct ttatcgacct ggtattccgc cacgaagggc
    18661 gttattacct gctcgactat aaatccaact ggctgggtga agacagttcg gcttacaccc
    18721 aacaggctat ggcagcagcg atgcaggcac accgctatga tctgcaatat cagctttata
    18781 ccctggcact gcatcgttat ctgcgccatc gcattgctga ttacgactat gatcttcact
    18841 ttggcggcgt tatctatctg ttcctgcgtg gcgttgataa agaacatccg caacaaggga
    18901 tttacacaac ccgacccaac gccgggttga ttgccctgat ggatgagatg tttgccggta
    18961 tgaccctgga ggaggcgtaa tgaaattgca aaagcaatta ctggaagctg tggagcacaa
    19021 acagctacgc ccgctggatg tgcaatttgc cctgaccgtg gcgggagatg aacatcctgc
    19081 tgtcaccctc gcggcggcac tgttaagtca tgatgccgga gagggacacg tctgtttgcc
    19141 gctttcacga ctggaaaata acgaggcgtc gcatccgctg ttggcgacct gtgtcagtga
    19201 aatcggtgag ctacaaaatt gggaagaatg cttgctggct tctcaagcgg tcagcagggg
    19261 agatgaaccc acgccgatga tcctctgtgg cgatcgtctt tatttgaatc gcatgtggtg
    19321 taacgagcgg acggtagcgc gcttcttcaa tgaggtgaat cataccattg aggttgatga
    19381 agctctactg gcgcaaaccc tggacaaact ttttccagta agcgatgaaa ttaactggca
    19441 aaaagttgca gcggcagtgg cgctgacgcg gcggatctcg gtgatttccg gcggcccagg
    19501 caccggtaaa acgaccaccg tggcgaagtt gctggcagcg ttaattcaaa tggccgacgg
    19561 cgaacgctgc cgtatccgtc tggctgcacc aacgggtaaa gctgccgcgc gcttaaccga
    19621 atctctcggc aaggctttgc gacagttacc gctgaccgat gaacaaaaga aacgcattcc
    19681 ggaagatgcc agcactttgc accgattgct gggcgcgcag ccgggtagcc agcgtttacg
    19741 tcatcatgcc ggtaacccgc tgcatcttga tgtgctggtg gtagatgaag cgtcaatgat
    19801 cgatctgcct atgatgtcga gactgatcga cgccttgcca gatcatgcgc gagtgatctt
    19861 tctcggcgat cgtgatcaac tggcctcggt tgaggctggg gctgtgctgg gcgatatctg
    19921 cgcttatgcc aacgcgggct ttaccgccga gcgtgccagg cagctaagcc gcctgacggg
    19981 gactcacgtt ccggcaggaa ccggcacaga agcggcatct ttgcgcgaca gtctctgcct
    20041 gctgcaaaaa agctatcgtt tcggcagcga ttctggcatt ggtcagttag ctgcggcgat
    20101 caaccgtggt gataaaacgg cagtgaaaac cgtttttcag caggatttta ctgatatcga
    20161 aaaacgactt ttacagagcg gcgaagatta tattgcgatg cttgaggaag ctcttgcggg
    20221 ttacggacgt tatctggatc tgctgcaagc gcgtgccgag ccggatttaa tcattcaggc
    20281 gttcaatgag taccagcttt tgtgcgccct gcgggaaggg ccgtttggcg tggctggact
    20341 gaatgagcga attgagcagt ttatgcaaca gaagcgcaaa attcatcgtc atccgcactc
    20401 tcgttggtac gaaggccgac cggtgatgat tgcccgtaat gacagcgcgc ttgggttgtt
    20461 taatggcgat atcggtattg cgctggatcg cgggcagggg acgcgcgtct ggtttgcgat
    20521 gccggacggc aatattaagt ctgtgcaacc gagtcgcttg ccagagcacg aaactacgtg
    20581 ggcgatgacg gtacataaat cgcagggatc ggagttcgac catgcggcgt tgattttacc
    20641 gagccaacgc acgccggtag taacgcgaga gctggtatac accgcggtga cccgcgcgcg
    20701 tcgtcgtctg tcgctgtatg ccgatgagcg tatattaagt gcggcaatcg ccactcgtac
    20761 tgagcggcgc agtggtctgg cggcgttgtt tagttcgcgg gaataaacgt aattgccgga
    20821 tgcgacgcac gagtgttacg cctgccgcat ccggtgattt tcatcgctta ccctaaatcc
    20881 gccatcaaca ctttggattt acgctggtag ttgtacaact gctttttgct ctcgggcagt
    20941 aaatcaatat ccaccggggt aaatccacgt tcctggaacc agtgaatact gcgcgtggtc
    21001 agcacaaaca atttgcttaa gccgctctgc ttcgcctgag cggcaatgcg ttccagcaga
    21061 acctcgcccc gtgatgaact gcggtaatcc gggtgaactg ccacacaggc catttcccca
    21121 atcttctctt ccgggaacgg atagagcgcg gcgcaggcaa tagtcgtgtt atcgcgctga
    21181 ataatggtga atttgtcgat ttccatctcc agctgctcgc gagaacggcg caccagaata
    21241 ccttgctgct ccagcgggcg aatcaactcc agaataccgc caatatcgtt gattgttgcg
    21301 cgacgaatct gctcggcgct ttccatcaca atctgcgtac cgataccgtc gcgtgagaac
    21361 aactcttgca acagcgcgcc atcttcctga taactgatta aatgacagcg gcgcacgccg
    21421 ctgcggcagg ctttcactgc gccacgcaaa aagcgcaccg taccggagtt gtaatcgcct
    21481 ttctcttcct gggcttctac ccgtgcttgc gcttcgttag ggaaaagttc ggagacaata
    21541 tcaccgtcgt cattagtaac gccctgggaa gagcaaaaac caatcatctt ttcagctttc
    21601 agtttgatgg ctagttgagt ggcaatctct tcagaggtca gattaaagct ctcgccagtg
    21661 actgaaacag cgaccggccc cattagcact attgcaccgc tttccagttg acgatggatc
    21721 gcgtcttcat caatccgccg gatacgcccg ctatggcagt aatccacgcc gtcatcgacg
    21781 cccagcggct gggcaataat aaaattgcca ctaacgacgt tgatatgcgc gccctgcagc
    21841 ggcgtgttat tgagactcat tgacaggcga gcagtaatat ccagttgcaa tgttcccgca
    21901 gcctgcttca ccagttccag tgttttggcg tcggtcacac gtatattctt gtgatacagc
    21961 ggttcgtggt gatgcgcagc cagatttgcg tcgatctgcg gacgtgcgcc atagaccacc
    22021 accagacgga tgccgaggct gtgcaacaac ccgatatcat taacgatact ggagaaattc
    22081 tcatgctcaa tggcttcacc gccgagcatg atgacaaacg tttttccccg gtgggtattg
    22141 atatagggaa ccgaatggcg gaatccctcg accagctcgg ttttacgttc ctttaccacg
    22201 gcacacccct ttgcatgatt atttgaaatt attgtatttt tattctgttt tttcgcaggg
    22261 tgcaagtgta aattttatgc gagagcgact tttttatcag taatgccgtg aatataaaaa
    22321 gaatgtttac cgtttattag atgacagatt atgcgtcttt cgctaaagtt tccggtcaaa
    22381 ttagtcgttt acttgttaca cagcttagat ttcttttgct cgggagaggc atgtcaggat
    22441 ccaacactgc aatcagccgt cgtcgtttac tgcaaggcgc gggcgctatg tggctattga
    22501 gcgtaagtca ggtcagcctg gctgcggtta gccaggtcgt ggcggtgcgc gtctggcctg
    22561 cgtccagcta cacccgcgtg acggtagaat caaatcgtca gctgaaatat aagcagttcg
    22621 cgttgagtaa ccctgaacgc gtggtggtgg atatcgaaga tgtaaacctg aactcggtac
    22681 tcaaggggat ggcggcacaa attcgtgcag acgacccgtt catcaagtcg gcgcgcgtcg
    22741 ggcaatttga cccgcaaacc gtacgcatgg tttttgaatt aaagcaaaac gtaaaaccgc
    22801 agctgtttgc ccttgcgccg gtcgccgggt ttaaagagcg tctggtgatg gacctctatc
    22861 cggccaatgc acaggatatg caggacccgc tgctggcgct gctggaggat tacaacaaag
    22921 gcgacctcga aaagcaggtg ccgccagcgc aaagtggtcc acaaccgggt aaagcagggc
    22981 gcgatcgtcc gattgtcatt atgcttgacc ctggccacgg tggcgaagat tccggtgcgg
    23041 tggggaaata caaaacgcgc gaaaaagatg tggtattgca aatagctcgc cgtttgcgct
    23101 ctctgatcga gaaagagggc aatatgaagg tgtacatgac gcgcaatgaa gacatcttca
    23161 ttccgttgca agtgcgcgta gcaaaagccc agaaacagcg tgctgacctg tttgtctcta
    23221 tccatgccga cgcctttacc agtcgtcagc cgagcggttc ctcggtgttt gcgctctcaa
    23281 ccaaaggcgc aaccagtact gcggcaaaat atctggcaca aacccagaac gcctcggact
    23341 tgattggtgg cgtgagcaaa agcggtgacc gctatgtcga ccacaccatg ttcgatatgg
    23401 tgcagtcgct gactattgct gacagcctta agtttggtaa agcggtactg aataagctcg
    23461 gaaaaatcaa caagctgcat aaaaatcaag ttgaacaggc cgggtttgcc gtactaaagg
    23521 caccagatat tccctccatt ctggtcgaaa cggcgtttat cagtaacgtt gaggaagagc
    23581 gtaaactgaa aacggcgact ttccagcagg aagttgcgga gtctattctt gcggggatta
    23641 aagcgtattt tgccgatggg gcaacgctgg cgagaagggg atgataaaaa ggcgctgaat
    23701 ggcgcttttt ttatgggaga tcagaaacaa aaaaacaccc gttagggtgt tcaataattg
    23761 gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg agctaccagg
    23821 ctgctccacc ccgcgtccgt ggatgcgcac tatactccgc tcgcgttttc atgcaacctt
    23881 ttttttaccc cgatcacgga tttagcatga ttttgaacaa aaacaaacca ctaaagctta
    23941 ttttttgtgc gaaatttgcg tcagggagag gctgctcatt tcattaaacg cggcggtttg
    24001 ttatcttcgt tgcgccttat tttttaacct gaagaagaga acaatgaaag gacgttgggt
    24061 aaagtacctt cttatgggca cggttgtggc aatgcttgcc gcctgctctt ccaaaccaac
    24121 cgatcgcgga cagcaatata aagacgggaa atttacccag cctttctctc tggtgaacca
    24181 gccagatgcc gttggcgcgc cgattaacgc cggtgatttt gccgagcaaa ttaaccatat
    24241 ccgtaattcg tcaccgcgtc tgtatggcaa ccagagtaat gtttataacg cggtgcaaga
    24301 gtggctgcgc gcaggcggtg atacccgcaa tatgcgccag ttcggcattg atgcctggca
    24361 gatggaaggt gccgacaact atggtaacgt gcagtttaca ggctattaca cgccggtaat
    24421 tcaggcgcgc catacccgcc agggcgagtt ccagtatcct atttaccgta tgccgccaaa
    24481 acgtggtcgt ctgccgtctc gtgcggagat ctacgccggg gcattgggcg ataaatacat
    24541 cctcgcttac agcaactctc tgatggataa cttcattatg gatgtgcagg gtagtgggta
    24601 tatcgacttt ggtgatggca gtccgcttaa ctttttcagc tatgcaggga aaaacggtca
    24661 tgcctatcgc agcattggta aggtgctgat cgaccgtggc gaagtgaaaa aagaagatat
    24721 gtcgatgcag gcgattcgtc attggggcaa aacacacagt gaagccgagg ttcgcgagct
    24781 gctggaacag aacccgtctt tcgtcttctt taaaccgcaa tcttttgcac cggtgaaagg
    24841 ggcaagtgcg gtgccgctgg ttggccgtgc ttctgttgcc tccgatcgtt caattattcc
    24901 gccaggtact accttgctgg cagaagtgcc gctgctggat aataacggca aatttaacgg
    24961 tcagtacgaa ttgcgtctga tggtggcgtt ggatgtcggg ggcgcaatca aaggccaaca
    25021 cttcgatatc tatcaaggga tcgggccgga agccggacac cgcgcaggtt ggtacaacca
    25081 ctatggacgg gtttgggtgc tgaaaacggc tccgggcgca ggtaacgtct ttagcggctg
    25141 atgtgatatt ctgtatgccg tttttacgga tatgacaggg tgaggggtaa cctcacatcc
    25201 tgattattga ctaagtgcgc gttgttcata ccggatgcgg cgtgaacgcc ttatccggcc
    25261 tacaaatcat gcaaatccaa aatattgcag gagttatgta ggcctgataa gcgtagcgca
    25321 tcaggcattt atgcgtttag ggtgagggat aacctcgccc tttttaattc tgaggtgcta
    25381 tgtctgtggt aattagtgat gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg
    25441 gtgaaaaagc gttgcaactg tttgctgacg cgcatatttg tgtggttggt atcggtggtg
    25501 tcggttcctg ggcggcggaa gcgctggcgc gcacggggat tggcgcaatc acgcttatcg
    25561 atatggatga tgtgtgcgtc accaatacca atcggcaaat tcacgctctg cgcgataacg
    25621 tcgggctggc aaaagcggaa gtcatggcgg agcgcattcg ccagattaac ccggagtgcc
    25681 gtgtaacggt ggtggatgat ttcgtgacgc cggataacgt agcgcaatat atgagtgccg
    25741 gttattcgta cgtgattgat gccattgata gtgtacggcc caaagcggcg ctgattgctt
    25801 attgtcggcg caataaaatc ccgctggtca cgaccggtgg cgcgggtggg cagattgacc
    25861 cgacgcagat tcaggttacc gatctggcga aaacgattca ggatccgctg gcggcgaagt
    25921 tgcgcgagcg cctgaaaagc gattttggcg tagtgaaaaa cagtaaaggt aagctcggcg
    25981 tggattgcgt gttttctact gaagcgctgg tgtacccgca gtcagacggt acggtgtgtg
    26041 cgatgaaagc cacggcagaa gggccgaagc ggatggattg tgcatctgga tttggcgcgg
    26101 caacgatggt gaccgccacc tttgggtttg ttgcggtttc tcatgcgctg aagaagatga
    26161 tggcgaaagc ggcgcgtcag ggttaaacaa tcaggtcgga tgagacgcga acgtcttatc
    26221 cgacccggtt ctgtgcttaa acctgcttcg tcgcagcgat aatcgcctcg cttaacgcat
    26281 ttaacccctg gctgcgtgag gcgctaagct gcgcacgtaa tcccagctca tcaaacaatg
    26341 ccagcggcga ctgtgcctgt aattcggcgg cggttttccc ctcaacggca gtcaacaaca
    26401 ccgccaacag gccgcgcaca atgcgccctt cgctgtcgcc aaagaagtgc attttgccgt
    26461 tttcagccac ggtatatccc agccagacgc ggttttcgca tccggcaatc tctttagcct
    26521 gcgcttttaa ctcgtctggc aaagccggaa gctgtttccc cagcatgatc aactggcgat
    26581 atttatcttc ccattgcgac aacggtgcga aggtattgcg taacgtttct gcggttacgg
    26641 ttgtgccgaa cggatgtccg gcgaattgcg ggtttgtcat taatccacca ataattccag
    26701 cgcgcggtca acggcattca ccagcgcatc cacatcactc tttgtattat atggcgcaaa
    26761 tgaggcgcgc agtgtgccgg ttacgcctaa ttctgccagt agcggctgag cgcaatgttg
    26821 cccggcccgc aaggcaatac cgtactccgc cagtagcgtc accatatcgc tgtgatgaac
    26881 gccagcaaaa tcaaaggcca gcaggctgga atcctggcag cggaatgaac gaaagcctgg
    26941 acgtttcgcc agcgcttctt ctgcaagcgt tgctaagcta cggctccagc tttcggcctg
    27001 gttgatatcg taatctgcca gccattccag cgccgcgctt aatcctatga cgccagcgac
    27061 atttggcgtt cctgcttcca gtttccacgg cgcagattga gtggtgaaac cgtcaaaact
    27121 cacttcgtga atcattttgc cgccacccag ccagggcgac atcgcttcca gcagttctga
    27181 tttgccatac agcacgccga tacccgtcgg gccatacagt ttgtgaccag agaaagcata
    27241 gaaatcgata tccagttgct gaacatccgc ggggaaatgc accgccccct gagcaccatc
    27301 aaccatcacc accatcccgg ctgaatgagc aaaggtaatc gctcgcgcca gatccgggca
    27361 accgccagtc acgttcgaca tctgacccaa cgccagaatc cgactacggg gagtaatcag
    27421 ttctggcaac aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt
    27481 ggctctagtt tgttgggcga ccatcagcca ggggacgagg ttagcgtggt gttctgccac
    27541 gctgacaata atctcatcgc ccggttgcag acgcggacgc gcatagcatt gtgccaccat
    27601 gttgatggat tcagtggtgc cgcgcgtcca gacgatagtt ttatcatccg gtgcattcag
    27661 taattgcgcc actttctctc gtgcagcttc ataacgcgcg gtcaggcgtt gggcttcggc
    27721 aaactggctg cgatggacgt ttccggcgct caggctgtaa aactgtcgag tggcttcaac
    27781 cacggcttca ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg catcctgtag
    27841 tgcgggaaac tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac tcctcggctt
    27901 gatgcaccga tcgtgacgca aaattcactt tgatacaagg agagtgctaa ccatcagaat
    27961 tatctagata tcctggcgaa gccgcgtaag taacatatgc tgaatagtgt taggcgttaa
    28021 ttttagcctg ttaatcaata ctattaagat ttattaatcg ctttaaggag aagatgatga
    28081 aaaagactgc cgcaattatt tctgcctgta tgctgacttt tgccctgagc gcctgttccg
    28141 gttcgaacta tgtgatgcac accaatgacg gacgtaccat cgtctctgac ggcaaaccac
    28201 agactgataa cgataccggt atgatttcgt ataaagacgc taatggcaac aaacagcaga
    28261 tcaaccgtac tgacgtgaaa gagatggtcg aactggaaca gtaattcgcg atcgcaaggt
    28321 aaaaaaaagc accgcaatta ggcggtgcta cattaatcac tatggacaga cagggtaaat
    28381 gtacaggaag tgaaaaaagg tagctttgct accatggtct gaatcgcaga ccaattgcaa
    28441 acacaacaac acaacatcac aaccgtaagc caaaagttca ccagaatacg cattccgata
    28501 aaacttttcg ttccggctca ggaagtgccg ccactatagg tatttgctgg tagaagctca
    28561 acggacaatt tataatggct cagattaaaa aaactaatag gttacacagt gtgatctaat
    28621 tgttaaattc atttaacatc aaagtttaaa agccatgtct aaacgattac caccgctaaa
    28681 tgccttacga gtttttgatg ccgcagcacg ccatttaagt tttactcgcg cagcagaaga
    28741 gctttttgtg acccaggccg cagtaagtca tcaaatcaag tctcttgagg attttttggg
    28801 gctaaaactg ttccgccgcc gtaatcgttc actcctgctg accgaggaag ggcaaagcta
    28861 tttcctcgat atcaaagaga tattttcgca attaaccgaa gcgacgcgta aactccaggc
    28921 ccgtagcgcc aagggggcgt tgacggtcag tttactcccc agtttcgcca ttcattggtt
    28981 ggttccgcga ctttccagct ttaattcagc ttatccggga attgacgttc gaatccaggc
    29041 ggttgatcgt caggaagata agctggcgga tgatgttgat gtggcgatat tttatggtcg
    29101 gggcaactgg ccggggctac gggtggaaaa actgtacgcc gaatatttat tgccggtgtg
    29161 ttcgccgcta ctgctgacag gcgaaaaacc cttgaagacc ccggaagatc tggctaaaca
    29221 tacgttatta catgatgcgt cacgccgtga ctggcagaca tatacccgac agttggggtt
    29281 aaatcatatc aacgttcagc aagggccaat ttttagtcat agcgccatgg tgctgcaagc
    29341 ggctattcac gggcagggag tggcgctggc aaataacgtg atggcgcaat ctgaaatcga
    29401 ggccggacgt cttgtttgcc cgtttaatga tgttctggtc agtaaaaatg ctttttatct
    29461 ggtttgtcat gacagccagg cagaactggg taaaatagcc gcctttcgcc aatggatcct
    29521 ggcgaaagcc gctgctgaac aagaaaaatt ccgctttcgt tatgaacaat aatttacgta
    29581 gggtacgacc atgaccagcc gttttatgct gattttcgcc gccattagcg gcttcatttt
    29641 tgtggcactg ggcgcttttg gtgcgcatgt gttaagtaaa accatggggg ccgttgagat
    29701 gggctggatc caaaccggtc tcgaatacca ggcgtttcat acgctggcga tcttaggtct
    29761 ggcggtggca atgcagcgtc gcatcagtat ctggttttac tggagtagcg ttttcctcgc
    29821 gttaggcacg gtgctgttca gcggcagcct ttattgcctg gcgctgtccc atctgcgttt
    29881 gtgggcgttt gtcacaccgg ttggcggcgt gagcttcctt gcgggctggg cgttaatgtt
    29941 agttggtgct atccgtttaa agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt
    30001 gccgtccggg ctttgaaaaa gagtgcgccg cagaaattac cgataaagcc ggccagcggg
    30061 aaattttcgg ttttgcccgc gtgaaagaga atgcgggtta tgtcatttat gaatgttatc
    30121 aacctgatga tggcgataag ttaatccgtg agctgccgtt cagttcatta atttttgccc
    30181 gccagtggtt tgtggtgggg gaactcctgc agcatttgcc gccagaagat cgtattaccc
    30241 ccattgtcgg catgttacag ggtgtagtag agaagggcgg tgaactgcgt gttgaagttg
    30301 ccgataccaa cgaaagcaaa gagttgctga aattctgccg taagttcact gttccgctac
    30361 gcgctgcctt gcgcgatgct ggggtgctgg cgaactatga aacgccgaag cgtccggttg
    30421 tgcatgtatt ctttattgca ccaggatgct gctataccgg ttactcatac agcaacaata
    30481 attcgccgtt ctatatgggc attccgcgcc tgaaatttcc ggcagaggcg ccgagtcgtt
    30541 ccacgctcaa actggaagag gcatttcatg tgtttattcc tgcggatgag tgggatgaac
    30601 gcctggcgaa cgggatgtgg gcggtggatt taggcgcttg ccctggcggc tggacctacc
    30661 aactggtgaa gcgcaacatg tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc
    30721 tgatggatac cgggcaggtg acgtggctgc gggaagacgg tttcaaattc cgtccgacac
    30781 gcagcaatat ctcctggatg gtatgcgata tggttgaaaa accggctaaa gttgcggcat
    30841 tgatggcgca gtggctggtt aatggctggt gccgtgaaac cattttcaat ctcaaactgc
    30901 cgatgaaaaa acgctacgaa gaagtatcac acaatctggc gtatattcag gcacagcttg
    30961 atgaacatgg cataaatgct cagattcagg cgcggcagtt gtatcacgat cgcgaagaag
    31021 tgacggtgca cgtccgccgc atctgggctg cggtgggtgg tcgtcgcgac gagcgataac
    31081 aggcaaggcc ggggcgtcgt ctccggcctg ctaccctttc cgtcaggctg ttaccaaaga
    31141 agttgcaacc tggcgctcat cagaaataat gtgcgttacc tcatccagat acccgatgcg
    31201 ggcttcccct gaacgattaa atttactttt atcaatcaat aacaacgatt gcgcggcacg
    31261 ttttaatagc atcgatttgt aatcagcgtt gatcgcattg gagtcccaca gtgcgccgct
    31321 gctatcgatc ccttcacaag aaaaaataaa cagatcgatt tccagcgatt taagttggga
    31381 aatcagcgag ggattgacgt aacagccata tttgcgctca agtgtgccgc cggaactgat
    31441 cagttgaatg cgttcgcgtt taccgagttc atggcaaatc ggatggctat tggtgaagac
    31501 ctgaatgttg atgtcaggca actggcgtgc cagataccag caagttgaac tggcgtctaa
    31561 ggctatcacc atcctttctt caatccacgc gagcgcctcg cgcgcgatat ctgctttatg
    31621 cgcataatgg cttttcagcc tgatgtgaaa gagagcgcta ctgtcttgat tttgacggtg
    31681 gatatattta gcgcgtccat gattgcgcag aattttaccc tgcgtctgta attcattgag
    31741 atcgcgacga atggtttctt tactgacctt tagctgttca gagagagctt ccgtggtcag
    31801 gctggtatgg ttcagcagca ggtcgactat cgcttgctgg cgtgccgctt tcatattttt
    31861 caccccatca ttcgataacc gcccgcgtca tgcgggcgta ccggtatgag attacggtgt
    31921 tacccctttt tttaaaagaa tattcccgta cttcgctcgt tcgcctgtga taacgatcgc
    31981 aaaggctttt tgcgcccgtt cataaaacgc aaaacgattg atgcggatga tgtcaggaca
    32041 cggggcttgt agtgaaagcg cattacggta acgtcgttct acttcaggat cgagagtgtc
    32101 accctctacc gccgccatca tcaccagcgg cggggcataa ctgtccagct caaataacgg
    32161 gataatcgcc tggagcaagt cgctcaccaa caggccatca gcgcggatca cctgcggtcc
    32221 catcgaatgg gcgggaaagt gagcatcgga aaaaataatt tcatctccat gtcccatctc
    32281 tgccagcact ttcaatagtt cgggagaaat taacggcgaa attgttttca gcatttcaca
    32341 cttcctctat aaattcaggt tcagtttgcg ggtagaaata acggtactga taatgaatct
    32401 gtgcacgggc ttcttccggg ctgttaaatt cccctacgcc ataccaaccg aacagcgcag
    32461 ctcctgcgac ggtcgtttcg gcatcgtcga gaacttttac cggaatatca agcatattgg
    32521 ctttaatctg attccacaat gtgttgcgac ttcctccacc gactaacaat aattcatagg
    32581 ctttaaagtg cccgattttt tccagcatct gtagattgcg ctgtaattgc gcagttaacc
    32641 cttccagcgc cgcgcgatag aaatgccccc gcgtggtatt aagcgtcact ccttgccagc
    32701 cagcgttctg acacgacaat aaatcacact gcattttcac accatccgcg ccaggtgcga
    32761 tcagacgagc ttcttcaatc aacatttgcc agggtgtttc agccgtccag aacagttttc
    32821 tcacccattc cagcacgccg gatgccagcc attgcatacc tgggttatac aaccatgcct
    32881 ggctatctag ttcgcaggtg gaaccggcgt actgacttaa cagcgaagta tcaacctagg
    32941 cgctgcgaac cattaaaatt tcccatgtac cggaggagag cacgggttca ttttgttcgg
    33001 caccagcgcc aaaaagggcg aactgggtat cgtgacctgc ggaaatcacc ggtatgccaa
    33061 cgggtaagcc gagcattgct gcggcgctgt tctgtagcgt accaatctgt tcacccgctt
    33121 ccaccagacg agggaagagt cggcgtggaa taccggtggc ttgtaaaatt tgcggactga
    33181 aatcgcgttg ctggatatcc agcatctggc tggttccggc tatcgtgata tcagtagtga
    33241 attcgccggt taaacggtgg ttaatcagcg acgaaatgaa gagccaggcg tgcgcgcgtt
    33301 ccagcagttg tggatgattt tctttcaacc acaccaactt atataacgta ttgaaactaa
    33361 aggctccgac gccagaaata gcctgcaacc gctgtgcgga gattaaccgt tcaatattgt
    33421 ccataaccgc tgctgttcgc ggacatttcc agctaatgat cggatagagc agattgcctt
    33481 gcttatctac cagagcgcca tccacaccaa aggtggtgac ggcgataccg cggatgtggc
    33541 attcagtcag ttcactattg atttgccgac agcaatcagc aaagcgttgc aaaatggcag
    33601 tctaaagacc actggtgcca ggtgttgttt tccatcgcga tatcgctggc attaggcgtt
    33661 gaggcgcggg caacaatttt tccctgccga ttaaccgcga tggccctgac attggtcgcg
    33721 ccacagtcga gtaccaggat aacttcttgt ttcataatcg ctcctgcaat atagccggat
    33781 aacattgttt atccggctaa ccactcttgc ttgttcaggc ccggtagccg gagcgaccgg
    33841 gcatcacatc agggtgtaat gtattaacgc ttgtacaacg gaccgtagtt ctggcaagcg
    33901 cggtaatcct ggccttcaat atccatgccg tgcgcagccc aggcagaagg acgatacact
    33961 ttggtctctt caacgttgtg catacatacc gggatacgca gcatggaggc aagggtgata
    34021 aagtctgcgc caacgtggcc gatggtcaga actccgtggt tggcacccca gttcgccatt
    34081 accgaataca catccgtaaa cggcccttta ccagtgaggc gcggtgcaaa ccaggtggtt
    34141 ggccaggttg agttggtgcg tttgttgagg atgtcatgca catccttcgg caattccacg
    34201 ctccagcctt ccgcgatttg cagtaccggt cccaggcctt tgatgatgtt gacacgagtc
    34261 atggtgaacg ggacgccgcc ttcggtaagg aagcgggaag agtaaccgcc gccacggaag
    34321 tattcgtgga tcgccgggca ccattcggta gcggcgaggc aagcgtcagc ctcttgctga
    34381 gagatttccc agtgcggctt catcgtcggg ttaccttcgc tgtcgcgttg tttacaggaa
    34441 ccgtccagcg cagcagaacc tgagttgatc aaatggatga tgccgtgttc tgccagtccg
    34501 tccagtttat gccccgttac acgctcaatt gcttctggtg accagtaggt acgcacatcg
    34561 gcaaatacct gagcggtgcc ggtgagctgg tgacccatta gcattgccac gccgttaaga
    34621 ctgtcgtttt cggtcgccac gacaaagggt tcgcgcacgc cattccagtc aaatgaactg
    34681 ttgaggatcg cttcggcggt gtcaccattg ggatattgat cggtccagtg acgttgcccc
    34741 tggaagcccg cagcgatggc gttgtagcca agtgattctt ccacgcgacc aatatcggcc
    34801 agtttgctgt tgccttgcat catgtcgcgg atacacatcg ccatcagtaa actttcgcgc
    34861 agaactgcgc ggctttgctc ggcattgcgt tgatactgtt tgttattttc atcttcgcca
    34921 tagcggaagt ttttatcagc ccaggccagt gccatttcca gttcggcttc gtcgtaaatc
    34981 ttctgatcga tacggcgacg cagttcggtc atatccaccg cctggacttt cattcccagc
    35041 caggattcaa agaagttgtg atcaacaata gaaccggcga tacccatcga aacgccgccc
    35101 agcgacagat agcttttacc tttcatgctg gcgacggcca aaccggcgcg ggcaaagcgc
    35161 agcagttttt cttcaacatc ggcaggaatc gatgtgtcat cggcatcctg aacgtcatga
    35221 ccgtaaatgg agaatgccgg gatgcctttc tggctgtgag ctgccagagc cgctgccagg
    35281 taaacagcgc cggggcgttc agtgccgtta aagccccaaa tggctttcgg gcgggttgga
    35341 tccatgtcga tggtttcact gccatagcac cagcaaggcg ttaccgtaat ggtgaggcct
    35401 acattctggc tgctgaattt ttcttcgcaa gcagcggctt cagccatacc cgcgatacag
    35461 gtatcggaaa tgacacactc gacggcagct ccgcaggtat ggcgcagttt ctcggtcagc
    35521 agtgcggccg tagctttcgc catattcatt gtttgttctt caagcgactc acgaacaccc
    35581 atgcgacgac cgtcaataac cgggcggata ccaattttcg gtaagctgat ttttttcatt
    35641 acagattcct cactttattc ggaaaatatg ttcagttagt tgccgtttga gaacggaaac
    35701 gggcaaagat aaagatgacc gcgaagcaga gtgcggggat cagttcagca gtggggatgt
    35761 tgcccgccgc gtcactgaca aaacccatga ccggagtgac aataccgccg ccaataatgg
    35821 tcataacgat gaaggacgaa ccgtatttgg tgtcctggcc gagattctta atgcccagcg
    35881 agaagattgt tgggtactga atcgacataa aggcgctgca taaagtcagg gctattaagc
    35941 ccacatgacc gccagcgaag gctgagatca ggcacagtgc catagcgatt aatgcgtagg
    36001 cggccaggac tttgtgtggt gcgaagcgac tgatgagcca ggtaccggtg atacgaccaa
    36061 taaagaagca caccatggtt ccggttaaat agttagcggc aaagcctgca gtcatacctg
    36121 gaatttcttc tacagcgtag cgaatcaaat agctccagca ggccgtttgt gcgccgacat
    36181 agcagaattg cgctaatacc gcccagcgcc agtgacgaat acgcgccaga cgagaaagcg
    36241 atgcggagaa cgatccttgt ttggcgtcac tgtgattatc actctgcaat gccgggaatt
    36301 tcgtcagcat gatcagcagg gcgaccagta acacgatagc cacgatgatc atataaggtg
    36361 tctgtaccga taatactagg ctgtgtttat acgcactcaa ttgctctgga gacattttat
    36421 cgagaacgtc ttgcgattga tgtggcacgt tagacaaaat aagactttgc ccaaagacaa
    36481 ccgcgataat tgcgcca
//
LOCUS       DAJTXY010000025        35843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTXY010000025 DAJTXY010000000
VERSION     DAJTXY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.025"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..218)
                     /locus_tag="OO566_001381"
     CDS             complement(<1..218)
                     /locus_tag="OO566_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6604725.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAEVY"
     gene            complement(219..305)
                     /locus_tag="OO566_001382"
                     /pseudo
     CDS             complement(219..305)
                     /locus_tag="OO566_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(399..1874)
                     /locus_tag="OO566_001383"
     CDS             complement(399..1874)
                     /locus_tag="OO566_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6604726.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3305)
                     /locus_tag="OO566_001384"
                     /pseudo
     CDS             complement(2021..3305)
                     /locus_tag="OO566_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3418..5352)
                     /gene="yeaG"
                     /locus_tag="OO566_001385"
     CDS             complement(3418..5352)
                     /gene="yeaG"
                     /locus_tag="OO566_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6604727.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGTLCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILPRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5788..6534
                     /gene="mipA"
                     /locus_tag="OO566_001386"
     CDS             5788..6534
                     /gene="mipA"
                     /locus_tag="OO566_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6604728.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6624..7385
                     /locus_tag="OO566_001387"
     CDS             6624..7385
                     /locus_tag="OO566_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6604729.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7436..8320)
                     /locus_tag="OO566_001388"
     CDS             complement(7436..8320)
                     /locus_tag="OO566_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6604730.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8404..9399)
                     /gene="gapA"
                     /locus_tag="OO566_001389"
     CDS             complement(8404..9399)
                     /gene="gapA"
                     /locus_tag="OO566_001389"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6604731.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9741..10154
                     /gene="msrB"
                     /locus_tag="OO566_001390"
     CDS             9741..10154
                     /gene="msrB"
                     /locus_tag="OO566_001390"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6604732.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10196..10468
                     /locus_tag="OO566_001391"
     CDS             10196..10468
                     /locus_tag="OO566_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6604733.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10838..11914
                     /locus_tag="OO566_001392"
     CDS             10838..11914
                     /locus_tag="OO566_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6604734.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11941..13320
                     /locus_tag="OO566_001393"
     CDS             11941..13320
                     /locus_tag="OO566_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6604735.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     SLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            13337..14382
                     /locus_tag="OO566_001394"
                     /pseudo
     CDS             13337..14382
                     /locus_tag="OO566_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14403..15239
                     /locus_tag="OO566_001395"
     CDS             14403..15239
                     /locus_tag="OO566_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6604736.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15244..16192
                     /locus_tag="OO566_001396"
                     /pseudo
     CDS             15244..16192
                     /locus_tag="OO566_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16202..17182
                     /gene="ydjG"
                     /locus_tag="OO566_001397"
     CDS             16202..17182
                     /gene="ydjG"
                     /locus_tag="OO566_001397"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6604737.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17319..18077
                     /locus_tag="OO566_001398"
     CDS             17319..18077
                     /locus_tag="OO566_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6604738.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18195..19553
                     /locus_tag="OO566_001399"
     CDS             18195..19553
                     /locus_tag="OO566_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6604739.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19646..20287)
                     /gene="pncA"
                     /locus_tag="OO566_001400"
     CDS             complement(19646..20287)
                     /gene="pncA"
                     /locus_tag="OO566_001400"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6604740.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20298..21314)
                     /gene="ansA"
                     /locus_tag="OO566_001401"
     CDS             complement(20298..21314)
                     /gene="ansA"
                     /locus_tag="OO566_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6604741.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TLDD"
     gene            complement(21481..23337)
                     /gene="sppA"
                     /locus_tag="OO566_001402"
     CDS             complement(21481..23337)
                     /gene="sppA"
                     /locus_tag="OO566_001402"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6604742.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23498..24049
                     /locus_tag="OO566_001403"
     CDS             23498..24049
                     /locus_tag="OO566_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6604743.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24166..25209
                     /gene="selD"
                     /locus_tag="OO566_001404"
     CDS             24166..25209
                     /gene="selD"
                     /locus_tag="OO566_001404"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6604744.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25214..27175
                     /gene="topB"
                     /locus_tag="OO566_001405"
     CDS             25214..27175
                     /gene="topB"
                     /locus_tag="OO566_001405"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6604745.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            27302..28342
                     /locus_tag="OO566_001406"
     CDS             27302..28342
                     /locus_tag="OO566_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6604746.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQLGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28459..29802)
                     /gene="gdhA"
                     /locus_tag="OO566_001407"
     CDS             complement(28459..29802)
                     /gene="gdhA"
                     /locus_tag="OO566_001407"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6604747.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            30038..30310
                     /locus_tag="OO566_001408"
     CDS             30038..30310
                     /locus_tag="OO566_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6604748.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(30276..30683)
                     /gene="nudG"
                     /locus_tag="OO566_001409"
     CDS             complement(30276..30683)
                     /gene="nudG"
                     /locus_tag="OO566_001409"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6604749.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30770..31453
                     /locus_tag="OO566_001410"
     CDS             30770..31453
                     /locus_tag="OO566_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6604750.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWFAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGY
                     LTLKSLQRQ"
     gene            complement(31462..32769)
                     /gene="ynjE"
                     /locus_tag="OO566_001411"
     CDS             complement(31462..32769)
                     /gene="ynjE"
                     /locus_tag="OO566_001411"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR6604751.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGGWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(32836..33489)
                     /locus_tag="OO566_001412"
     CDS             complement(32836..33489)
                     /locus_tag="OO566_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6604752.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33489..35023)
                     /locus_tag="OO566_001413"
                     /pseudo
     CDS             complement(33489..35023)
                     /locus_tag="OO566_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34996..>35843)
                     /locus_tag="OO566_001414"
     CDS             complement(34996..>35843)
                     /locus_tag="OO566_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6604753.1"
                     /translation="KEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGA
                     QLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPA
                     ALKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTF
                     NPAHAQQKIASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDA
                     QLRKADPVVWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLR
                     RYGTH"
BASE COUNT         9081 a         8483 c         9179 g         9100 t
ORIGIN      
        1 cagtacacct cagcgagagc gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 ctcatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gcgtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtctgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaat atttcattaa catgaaaaga
     1561 aaaatgattg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgattgt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgcaca caatgtctga
     2161 tgtgcacgac gggtaatttc gatatagctg taataacgaa caacaggtaa taattttttc
     2221 gccaggattt catggcaaag cggagagtca tcggcccagt tatcgccgtc cgatgcttgt
     2281 gcagcgtaaa tattccactg tgccgggtta taacgctctt tcactacctc atccatcagt
     2341 ttcagggcgc tggaaacaat ggtgccgcct gtttcctgcg agtagaaaaa ctcatgttca
     2401 tcgacttctt tcgcctgggt atgatggcgg atgtatacga cttccacgtt cttatacgtt
     2461 ctgctgagga acagatacag cagaatataa aaacgcttag ccatatcttt agtggattga
     2521 tccattgaac cggaaacgtc catcaggcaa aacatcactg cctggctgga gggatcgggc
     2581 cgcttctcgt agttcttgta acgtaaatcg aaggtgtcaa taaaagggac gcgttcaatt
     2641 ttggcacgta attctgcaat ttctttgcgc agacgttcct cttccagcag ctgcgcaggt
     2701 tcactgttgc tgatgatggc caaattctct tccagtgcat gaagttcccg ccgcttgcct
     2761 gccgtcatgg ctgtgcgtcg cgccagtgag ttctgcaagg aacgcacaac gctgatattg
     2821 gctggaacgc cgttagcggt ataacccgcc cgatgcgttt tatattcggt cagctggcgt
     2881 tgttggtttt gtttcagatt cggtaaggcc aaatcttcaa agagcagatc aagatactca
     2941 tctttcgaaa tctgaaagac aaattcatcc tgaccttcac catcctggct ggcctggccc
     3001 tgaccactgc cggaacctcc gccgccaccc tggggacgtt caattcggtc gttctgaacg
     3061 aaatggtcat tgcccggatg cacgcggtgg cgcagaccgc cacgcccctg atgaaacatt
     3121 ggttcgctaa tatcttccgt gggaatggat acggattcgc tgctgtcgac gtcagtcacc
     3181 gaacgcttat taatggcctc ggagatcgac tgtttaattt gcgctttata acggcgtaaa
     3241 aaacgctggc gattcaccat gcttttgttt ttgccgttca gacgccggtc aataaaccag
     3301 gtcatatgcc ccccgtacta catttgccaa ctgtatgcta cgcctgtgtt gccgcttctc
     3361 aggcccacgg ttcgctatcg ttgtaggccc ggtagcatgt gtaccgggcc agggttgtta
     3421 agacgattta cgtacgcgca aataccattc gcacagtaaa cgcacctgtt tacgggtgta
     3481 gcctttttcc atcatacggt cgacaaagtc gtcgtgtttc ttctgctcgt cggttgacgt
     3541 tttggcgtta aacgagataa ccggcaacag ctcctcggta ttggagaaca ttttcttctc
     3601 gataaccgtg cgcagttttt catagctggt ccagttcgga ttgcgtccgc tgttattcgc
     3661 tctggcgcgc agtacgaagt taacaatctc gttgcggaaa tcttttggat tactgatccc
     3721 cgccggtttc tcgattttct ccagctcggc gttaagagac tcgcggtcaa acagctgccc
     3781 ggtatccgga tcgcgatact cctgatcctg aatccagaaa tccgcgtagg taacataacg
     3841 gtcgaaaatg ttttgcccat attcggaata ggattcaagg taggccgtct ggatctcttt
     3901 gccgataaac tcggcatatt tcgggatcag ataacctttc aggaactcca gatagcgttc
     3961 tgcctgctct tgcgggaact gctcgcgctc gatctgctgt tccaggacgt agaacagatg
     4021 gaccgggttt gccgccactt ctacatgatc gaagttgaac acgcggggga ggatcttaaa
     4081 cgcaaaacgc gtcgacagac cgttcatccc ttcatcgaca ccggcgtagt cacgatattc
     4141 ctgatacgac ttggctttcg gatcggtgtc tttcagactt tcgccatcat aaacccgcat
     4201 ctttgaataa atgctggagt tttctggctc tttcaggcgc gaaagaatgg aaaaacgtga
     4261 cagtgtttcg agcgtgccag gggcgcatgg ggcgtgagtc aattcactgt gattaagcaa
     4321 tttctcgtag attttgatct cttcggaaat gcgcaagcaa tacggcacct tcacgatgta
     4381 aacacggtcg aggaaggctt cgttgttttt gttattacgg aaagtgaccc attcggactc
     4441 gttcgagtgg gcgagaataa tcccgttgaa cggcagggcg gagatacctt ccgtcccgtt
     4501 gtagttatct tcctgagtgg cggttaacaa gggatgcagc acttttatcg gtgctttaaa
     4561 catctcaacg aattccatga tcccctgatt ggcgcggcac agcgtaccgg aatagccgta
     4621 ggcgtccggg tcattctgcg cgtagtgttc gagtttacga atatcgactt tcccaaccag
     4681 cgcggagatg tcctggttgt tctcatcacc gggttccgtt ttggcgatag caatttgttg
     4741 cagaattgac ggccagacct tcactacccg gaacttagtg atatcgccac caaattcatg
     4801 caggcgtttt gccgcccatg gcgacatgat ggtgccgaga taacggcgag gaatgccata
     4861 ctctttttcc agaatctgag catcttcctg cggattgaaa agacagaacg gatgatcgtt
     4921 gaccgggcta cgctcaccgt tggcgctcaa tacataaatc ggtacgagct gcattaatga
     4981 tttcagtcgc tcagcaagcg atgatttacc cccacccaca ggccccagca gatacaggat
     5041 ttgtttcttc tcttccagcc cctgagccgc gtgtttcaga taagagacaa tctgttcaat
     5101 cgcgtcttcc atgccgtaaa actcttcaaa tgccggataa cgtgcaatga cccggttaga
     5161 aaagagtcga gaaagtctgg gttcctgggc tgtatcgacc atgacaggct caccgatagc
     5221 catcaatagc cgctcagccg cgttggcata agcactgcga tcttgccgac aagtggtaag
     5281 aaactcctgc agtgtgaact cttcgtcctt ggcagcttca tatcgctggc gatagtgatc
     5341 gaatatattc atggtatgcc gtcctttcgt tttttagcac aggttaagag ccgttcatat
     5401 gaatagtaga ggctcccgga agaggtaaac tgaacgacgt ctcttgctaa aacacagcaa
     5461 ctctcatgcc attttatcga cgatgagaac aacgtgcccg gtgatacacc ttcaaaatta
     5521 agcgtagatg gcaaatggaa aacttgcctg cacggctttc ttaatttcaa tggcatatca
     5581 acaactcatc cataaatttt gcataattaa tgtaaagacc aggctcgcca gtaacgctaa
     5641 atccatttgg ctgtaagcgc ggtgtcatcc gcggcaggaa aattaaacag ttactttaaa
     5701 aaatgaaaac gtaaaaaggt tgggtttcga tgtattgacg ggtaaacttt gtcgcccgct
     5761 aaacatttgt ttaaggaatg attaattgtg accaaactca aacttctggc acttggagtg
     5821 cttatcgcaa cgtctgcagg cgtagcgcac gctgaaggta aattttccct gggcgcaggc
     5881 gtaggtgtcg ttgagcaccc atataaagat tacgataccg atgtttaccc agtaccggta
     5941 atcaactatg aaggcgataa cttctggttc cgtggcttag gtggtggtta ctacctgtgg
     6001 aatgacgcaa cggataaact ttcaattacc gcttactggt cgccgcttta cttcaaagcg
     6061 aaagacagtg gcgatcacca aatgcgtcac ctggatgacc gtaagagcac catgatggct
     6121 ggtctgtctt atgctcactt tacccagtac ggttacctgc gtaccaccct ggctggcgat
     6181 accctggata acagcaacgg cattgtctgg gatatggctt ggttgtatcg ttacaccaac
     6241 ggtggcctga ccgtgactcc gggtattggt gtgcagtgga acagcgaaaa ccagaacgaa
     6301 tactattatg gcgtatcgcg caaagagtcc gctcgcagcg gtctgcgtgg gtataaccca
     6361 aatgacagct ggagccctta ccttgagctg agcgccagct acaacttcct cggcgactgg
     6421 agtgtttacg gtaccgcgcg ctacacccgt ctgtctgatg aagttactga cagcccgatg
     6481 gtggataaat cctggactgg cctgatttct accgggatca cctacaaatt ctgataatgc
     6541 atctttccag ggcattttgt gcataaaaat agggcgtcac gccctgtttt gcattacggt
     6601 ggtgcaatac ctgggaggaa acaatgcaac aaaaaatgat tcaatttagt ggcgatgtct
     6661 cactgccagc catagggcag ggaacatggt atatgggcga agatgccagt cagcgcaaaa
     6721 cagaagttgc tgcactacgc gcaggcattg aactcggttt aaccctcatt gataccgccg
     6781 aaatgtatgc cgatggcggt gccgaaaagg tggttgggga agcattaacc ggtctgcgag
     6841 agaaggtctt tctcgtctct aaagtctatc cgtggaatgc tggcgggcaa aaagcgataa
     6901 atgcatgcga agccagttta cgccgtctca atactgatta tctcgatctc tacttattac
     6961 actggtctgg cagtttcgct tttgaagaga ctgttgctgc gatggaaaaa ttgatcgccc
     7021 agggaaaaat ccgtcgctgg ggcgtttcta accttggtta tgccgatatg caggaactct
     7081 ggcagctgcc ggggggaaat cagtgtgcca ctaatcaggt gctttaccat ctcggttcac
     7141 gagggattga gtacgatcta ctcccctggt gccagcaaca gcagatgccg gtgatggctt
     7201 acagtccatt agtccaggcc gggcggttgc gcaatggact gttaaaaaac gcggtagtca
     7261 acgaaattgc acatgtccaa caaaatgcag ctgcgcttga ggtcgaactt tcgtcagcgg
     7321 aattagctat gctggataag gcatatccgg caccaaaagg aaaaactgcg ctggatatgg
     7381 tgtgatgttg atacgtgccg gatgcatcgc acccggcaca caacattaac gcaaattaac
     7441 gtttggcaac gcgaatggat tgcgccagat gtgcaggttt ctctttggtg actttttgcg
     7501 tttctgaagc gtaagccgtt tctacacaaa caaaggtctt gtagccatcg tccggcatgt
     7561 cgcccatgct aactgaaagc gccggtcccg ggttccagcc gacaacgttc agatgatgct
     7621 ggtggcctac ggcgataata cgattcagcg cttcatcatt aatcacgctg caatcttgcg
     7681 gattcagata cacgcgatcg gtacggtcag ggaaggtctg aataccgtcg gtcagtacat
     7741 cttctttcgc gtcattcact ttatcaatga agcgatcgcc cagcccactg acgcttacct
     7801 tagcgatatc acccacgtta aagtaggtat gcagggcaga ggtggtttca aattcgccat
     7861 gtgattcaag atcgatttca caggttttac ccacgcggaa atgcgctaac agcgtaaagt
     7921 cgtgcggcca gaattttttc gtctcttcgc tttgtgtcaa ttcaaaagtc agcgctacgc
     7981 catcagcatc ttcatgatgt gatttcagcg tccacggcag gttgcgggca aaaccgtgcg
     8041 caggcagacc ttgttgtgcc gccggaccaa accacggcca gcaaaccggt acgctaccgc
     8101 gaatagcgac gccatttttg aacggtgtgt tgttgctcaa ccacagaact tcttcttcac
     8161 ccgcaggttt ccacgagaga aggtgtgcgc cctgtaatgc aaaagaggct tttacctggg
     8221 gatgatcgac cacaatgagg tccagttcat ccagtttacg acgggagagg acaggggaga
     8281 tttgttcgat gaccggaagg gcaaaaattt tcttaatcat gacgcagtcc tttaacttca
     8341 ttttatcagg taaaaaaaag agcgaccgaa gtcgctcttt ttagatcaca gtgtcatctc
     8401 aacttatttg gagatgtgag cgatcaggtc cagaactttg ttggagtaac cggtttcgtt
     8461 gtcgtaccaa gataccagtt tcacgaagtt gtcgttcaga gcgataccag ctttagcatc
     8521 gaacacggaa gtgcaaactt cgccgttgaa atcggtagat actacgtcat cttcggtgta
     8581 gcccagaacg cctttcattt cgccttcagc agcagcttta acggcagctt tgatctgctc
     8641 gtaagttgca gctttttcca gacgaacggt caggtcaact acggatacgt tcggggtcgg
     8701 aacgcggaac gccataccag tcagtttgcc attcagttct ggcagtactt tacctacagc
     8761 tttagcagca ccggtagagg acgggatgat gttctgggaa gcgccgcggc cgccgcgcca
     8821 gtctttgtga gacgggccat caacggtttt ctgagtagcg gtagtagcgt gaacggtggt
     8881 catcagacct tcgatgatgc cgaagttatc gttgataact ttagccagcg gagccaggca
     8941 gttggtggtg caggaagcgt tggaaacgat gtcctggcca gcatatttgt cgaagttagc
     9001 gcctttaacg aacatcggag tgttgtcttt agacggacca gtcataacca ctttcttcgc
     9061 accagcggtg atgtgtttac gagcagtttc gtcagtcagg aacagaccag ttgcttcagc
     9121 gacaacgtca acacccactt cgtcccattt caggttagcc ggatcacgtt cagcggtaac
     9181 acggattttt ttaccgttaa cgatcagatg accgtctttc acttcaacgg taccgtcgaa
     9241 acggccgtga gtggagtcat atttcagcat gtatgccatg taatcagcgt ctaacaggtc
     9301 gttgattgca acgatctcga tgtcagaacg tttctgagca gcacggaaaa caatgcgacc
     9361 gatacggcca aaaccgttga tacctacttt gatagtcata tattccacca gctatttgtt
     9421 agtgaataaa aggttgcctg taaaattaca aaaaccttac gcagcgtcaa gcggaatcgt
     9481 gtcaatcatt gcgacagatc aatcctgtgc ctaagcatta cgcgactgac tcgcctcact
     9541 cttcctttgg gcttgagacc acatggggtc ggcgccccga attttaaagg gcaattacga
     9601 taaaaatgtg attttcatca cgatttaggt gcagccattt cgtcgagatc aagtttagga
     9661 caaaattttg ccagttggtg ttaatgtttt gttagaatca gtcacgtaat gtgagcacga
     9721 ttaaagtgag atgtgagtaa atggcgaata aaccttcggc agaagaactg aaaaaaaatt
     9781 tgtccgagat gcagttttac gtgacgcaga atcatgggac agaaccgcca tttacgggtc
     9841 gtttactgca taacaagcgt gacggcgtat atcactgttt gatctgcgat gccccgctgt
     9901 ttcattccga aaccaagtat gattccggct gtggctggcc cagtttctac gaaccggtga
     9961 gtgaagaatc cattcgttat atcaaagact tgtcacatgg aatgcagcgc atagaaattc
    10021 gttgcggtaa ctgtgatgcc catctggggc atgtctttcc cgacgggccg caaccaacag
    10081 gcgaacgtta ttgtgttaac tctgcttctt tacgcttcac tgatggcgaa aacggcgaag
    10141 aaatcaacgg ttgaaaaaac gattcagcaa cttattccac aggagcggaa aaattatgaa
    10201 tcttgatgac attatcaaca gcatgacgcc tgaggtatac cagcgtttgt cgaccgctgt
    10261 tgaactgggg aaatggcctg atggcgttgc gttgaccgag gaacaaaaag agaattgcct
    10321 gcaactggtg atgctgtggc aagcccgcca taataccgaa gcacagcata tgacaattga
    10381 cacaaatggt cagatggtca tgaagagcaa acagcagtta aaagaagatt ttggtatctc
    10441 agcaaagcct attgccatgt ttaagtaagt ggttcatccc ctttttgccg cggctatgaa
    10501 ggggatagcc aacaatgacc ccacgactcg tctcaattat ctcgacccga cctggctaag
    10561 tcccagtccg atctcttgca gtcgcgttca cgcctgaatg acttctcctg aacttaagtc
    10621 tcagacctat ttggccggta atccctctcg aatttgctct ttttgcgtcc cgccttccac
    10681 caacctgtaa cagacccagc cattttctgc gtaattgtga tctaagttgt aaaatgcagg
    10741 tcattatctg tgttgtgcag atttaatgtt gacctcaaaa atcatgtggg taagtatgat
    10801 ggccatctaa gcaaaacaaa acacagagga tgacgcaatg aaagcactgg ctcggtttgg
    10861 caaggccttt ggcggctaca agatgattca tgtcccacaa cccatttgtg gcccggaaga
    10921 cgtagtgatt gaaattaaag ccgcggcaat ctgcggcgca gacatgaagc actacaatgt
    10981 cgatagcggt tctgatgagt ttaactctat ccgcggccat gagttcgcag gttgtattgc
    11041 gcaggttggt gaaaaagtca aagactggaa agtggggcaa cgcgtcgtgt cggataacag
    11101 cggtcacgtt tgcggcgttt gtccggcctg tgaacagggt gattttctgt gttgtacaga
    11161 aaaagtaaac cttggtctgg ataacaatac ctggggcggt ggtttttcca aatattgtct
    11221 ggttcctggt gaaattctca aaattcatcg tcatgcgttg tgggaaatcc ctgatggtgt
    11281 tgattatgag gacgcagccg tacttgaccc tatctgtaat gcctataaat ccatcgcgca
    11341 gcaatcgaaa ttccttcctg gtcaggatgt tgtcgtcatt ggcactggcc cactcgggct
    11401 gttttccgta caaatggcgc ggattatggg ggcggtaaat atcgtcgtcg ttggtctgca
    11461 agaagatgtg gcggtccgct tcccggttgc aaaagaactg ggggcgacgg cagtagtaaa
    11521 tggttctacc gaagatgtgg tggcgcgctg ccagcaaatt tgtggcaaag ataatctggg
    11581 actggtgatt gaatgctccg gtgccaatat cgcactgaaa caagccatcg aaatgctccg
    11641 cccgaatggg gaagtggtac gcgttggaat gggcttcaaa cctcttgatt tctcgattaa
    11701 tgacattacc gcctggaata aaagcatcat tgggcatatg gcctatgact ccacctcatg
    11761 gcgtaacgct atccggctat tagccagcgg cgctatcaaa gtcaaaccga tgatcacgca
    11821 tcgtatcggc ctgtcgcaat ggcgcgaagg gtttgatgcg atggtcgata aaaccgcaat
    11881 caaagtgatc atgacttacg actttgatga ataactactc tcctgaagga acgttatcgc
    11941 atggaacaga taacaaaacc gcattgtggt gcacggctgg atcgcttacc ggattgccgc
    12001 tggcattcgt caatgtttgc catcgtcgcg tttggcttgc ttgtctgctg gagtaatgcc
    12061 gttggtggct tgatcctcgc gcagctgaaa gcgttgggct ggacagataa ttccaccaca
    12121 gccacattct cagcaatcac gaccgccgga atgtttctcg gtgcgctggt gggcggcatc
    12181 attggcgaca aaaccggtcg cagaaatgcg ttcatcctct atgaggccat tcatattgcc
    12241 tcgatggttg tcggtgcttt ctcaccgaat atggattttc ttattgcctg ccgttttgtg
    12301 atggacgttg ggctgggagc tttactggtt acactgtttg ctggtttcac cgaatatatg
    12361 cccggtagaa atcgcggaac gtggtccagt cgggtttcct ttattggcaa ctggtcatat
    12421 tcgctctgct cactgattgc gatgggactc acgccgctga ttagtgcaga gtggaactgg
    12481 cgggtacaac tgcttattcc tgcaatattg tcgcttatcg ctacggcgct ggcctggcgc
    12541 tactttcctg aatccccgcg ctggctggaa tcgcgcggac ggtatcagga agccgagaaa
    12601 gtcatgcgga gtatagaaga aggcgtcata cgccagacgg gtaaaccttt gccgcctgtg
    12661 gttattgctg atgacggtaa agcgccacaa gcggtgccgt attcagcctt actgacagga
    12721 gtattactga aacgcgtgat attaggctct tgtgtgctga ttaccatgaa cgttgtgcag
    12781 tacacactaa ttaactggtt gccaacaata ttcatgaccc aggggattaa tttaaaagac
    12841 tcgattgttt taaataccat gagtatgttt ggcgcgccat ttggtatttt tattgccatg
    12901 ctggtgatgg ataaaattcc gcgtaaaaca atgggtgtgg ggctattaat cctgattgcg
    12961 gtgctcggat atatctattc actgcaaacc agtatgttgc tcataacgct gattggtttc
    13021 ttcctgatta ctttcgtcta tatgtacgtt tgctatgcct cggcagtgta tgtccctgaa
    13081 atctggccga cagaggccaa actccgtggc tccggtctgg cgaatgcggt agggcgaatc
    13141 agtggtattg ccgcacctta tgccgttgca gtgctgctca gtagttatgg tgtaacggga
    13201 gtctttattc ttctgggggc tgtttcaatt attgtcgcaa ttgctatcgc caccattgga
    13261 attgaaacca aaggtgtctc cgttgaaagt ttaagtattg atgcagtagc caataaataa
    13321 tacgggtgga ttcaaaatga aaaattcaaa agcaatattg caggtgccgg gcacaatgaa
    13381 aattatttca gcagaaatac cagtgcctaa agaagatgaa gttttgatta aagtagaata
    13441 tgtcggtatt tgtggttcag atgtacatgg ttttgaatca ggcccgttta ttccgcctaa
    13501 agacccaaat caagaaattg gcctgggtca tgaatgcgcc gggacggttg tggctgtggg
    13561 aagccgcgtg cgcaaattta aaccggggga tcgggtaaat atcgaacctg gcgttccttg
    13621 cggtcactgt cgttactgtc tggaaggcaa atataacatc tgcccggacg ttgattttat
    13681 ggcgacacaa cccaactacc gcggcgcatt aacgcactat ctgtgtcatc cggagagctt
    13741 tacttacaaa ctgcccgaca atatggacac gatggaaggg gcgctggtgg agcctgccgc
    13801 agtcgggatg catgccgcga tgctggcaga tgttaaaccg ggtaagaaga taattattct
    13861 gggagcaggt tgtattggtt tgatgacgtt gcaagcgtgc aaatgcctgg gcgcaacgga
    13921 aattgccgtc gttgatgtac tggaaaaacg tctggcaatg ggggaacagc ttggtgcgac
    13981 agtggttatt aatggcgcaa aagaggacac tattgcacgc tgtcagcaat ttaccgaaga
    14041 catgggcgca gatattgttt tcgaaacagc gggttctgcg gtcaccgtta aacaggcacc
    14101 tttaatctgg taatgcgcgg cggtaaaatt atgattgttg gtactgtacc cggcgattcg
    14161 gcaatcaatt tcctcaaaat caatcgcgaa gtcactatcc agacggtatt ccgctatgcc
    14221 aatcgttatc cggtcacgat tgaagctatt tcttcagggc gattcgatgt gaaatcgatg
    14281 gtgacgcata tttacgatta tcgggatgta caacaggcat ttgaagagtc agttaacaac
    14341 aagcgcgaca ttattaaagg cgttattaaa attagcgatt aattaatacg ggagaacaga
    14401 acatgctcgc agatatcaga tattgggaaa acgatgccac aaataaacat tatgcaattg
    14461 cccattttaa cgtatggaat gcagaaatgt tgatgggcgt taaagacgca gccgaagaag
    14521 cgaaatcccc ggttattatt tcttttggta caggttttgt cggtaacacc tcatttgaag
    14581 atttctctca catgatggta tcaatggcac aaaaagcaac ggtgccggta ataactcatt
    14641 gggatcatgg tcggagtatg gagattattc ataacgcctg gactcatggt atgaattcat
    14701 taatgcgtga tgcttctgca tttgatttcg aagaaaatat tcgtttaacc aaagaggctg
    14761 ttgatttctt tcatccgctg ggtattccgg tagaggcgga attagggcat gtcggtaatg
    14821 aaaccgttta tgaggaagcg ctggcgggct atcactatac cgatcctgac caggcggctg
    14881 aatttgttga acgtacgggc tgtgactcat tggctgtcgc catcggcaac cagcatgggg
    14941 tttatacgtc agagccgcaa ttgaactttg aggtcgtcaa acgcgtacgc gatgctgttt
    15001 ctgttccgct ggttttgcat ggcgcatcgg ggatcagtga tgccgacatt aaaactgcaa
    15061 tttcgttggg tattgcgaaa atcaacattc atacggagct ctgtcaggcc gcaatggtcg
    15121 cagtgaaaga aaaccaggat cagcctttcc tgcatttaga acgtgaagta cgtaaagctg
    15181 taaaagaacg ggcattggaa aaaattgaac tgttcggttc tgatggcaaa gcggaataat
    15241 gatatggata atctcgacgt tatttgtata ggtgccgcta ttgttgatat tccattacaa
    15301 ccggtcagta aaaatatctt tgatgtagat tcttatcctc ttgaaagaat cgcaatgagc
    15361 accggggggc gatgcaataa atgaagcaac aattatttct cgtctggggc atcgtaccgc
    15421 attaatgagc cgcattggta aagatgcctc agggcaattt attctcggcc attgtcgcaa
    15481 agagaatatt gatattcaaa gcctgaaaca ggacgtcagc atagatacat ctattaacgt
    15541 tggactggtg acggaggatg gcgagcggac gtttgtcacg aaccgtaacg gcagtttgtg
    15601 gaaattaaat attgacgatg ttgattttgc gcggtttcct caggcgaaat tattatcact
    15661 ggccagtatt ttcaacagtc ctctattgga tggtaaagcg ctaacagaaa tttttacgca
    15721 agcgaaagcc cggcagatga tcatttgtgc cgatatgatc aaaccgcggt tgaatgaaac
    15781 gctggatgat atttgcgaag cattgagtta tgtcgattat ctgtttccaa attttgccga
    15841 ggcaaaatta ctcacaggga aagagacact ggatgaaatt gctgactgct ttcttgcgtg
    15901 cggcgtaaaa acggtggtga ttaaaacggg taaagacggc tgctttatca agcgtggtga
    15961 catgacgatg aaggtgccgg cggtcgcagg aataaccgcc atcgacacca ttggcgcggg
    16021 cgataacttt gcttcaggtt ttattgcggc actgttagaa ggcaaaaatc tgcgtgaatg
    16081 cgcacgcttt gccaatgcaa cggcggctat ctcggttcta agcgtcggtg ccaccaccgg
    16141 cgtaaaaaac agaaagctgg tcgaacaatt gctggaagaa tacgaaggat aatgaaggca
    16201 aatgaaaaag atacctttag gcacaacgga tataacgctt tcgcgaatgg ggttggggac
    16261 atgggccatt ggcggcggtc ctgcatggaa tggcgatctc gatcggcaaa tatgtattga
    16321 tacgattctt gaagcccatc gttgtggcat taatctgatt gatactgcgc taggatataa
    16381 ctttggcaat agtgaagtta tcgtcggtca ggcgttaaaa aaactgcccc gtgaacaggt
    16441 tgtagtagaa accaaatgcg gcattgtctg ggaacgaaaa ggaagtttat tcaacaaagt
    16501 tggcgatcgg cagttgtata aaaacctttc cccggaatct atccgcgaag aggtagaagc
    16561 cagcttgcaa cgtctgggta ttgattacat cgatatctat atgacgcact ggcagtcggt
    16621 gccgccattt tttacgccga tagctgaaac tgtcgcagtg cttaatgagt taaaggctga
    16681 agggaaaatt cgcgctatag gcgctgctaa cgtcgatgct gaccatatcc gcgagtatct
    16741 gcaatatggt gaactggata ttattcaggc gaaatacagt attctcgacc gggcaatgga
    16801 aaacgaactg ctgccgctat gtcgtgataa tggcattgtg gttcaggttt attccccgct
    16861 agagcaggga ttgttgaccg gcaccatcac tcgtgattac gttccgggcg gcgctcgggc
    16921 aaataaagtc tggttccagc gtgaaaacat gctgaaagtg attgatatgc ttgaacagtg
    16981 gcagccactt tgtgctcgtt atcagtgcac aattcccact ctggcactgg cgtggatatt
    17041 aaaacagagt gatttaatct ccattcttag tggggctact gcaccggaac aggtacgcga
    17101 aaatgtcgcg gcactgaata tcaacttatc ggatgcagac gcaacattga tgagggaaat
    17161 ggcagaggcc ctggagcgtt aaatactcaa cacgattcac ctctgtttct gtgaaaactg
    17221 tgatgaaaat ctgtgattca aataggttat tttgaaagta aacatcggtt atgcgataat
    17281 cgcgctaatg tgatgtaaaa agatacaggg gtgtaatcgt ggcggcaaaa gacaggattc
    17341 aggcaattaa gcaaatggtt gccaacgata aaaaggtgac agtctcaaat ttgagtggga
    17401 tttttcaggt aaccgaagaa accattcgcc gcgatcttga gaagctggaa gatgaaggct
    17461 ttttgaccag aacctatggt ggtgctgttt taaatacagc ggtgttgacg gagaatatcc
    17521 atttttataa gcgcgcttca tcgttttatg aagagaagca gcttattgca cgcaaggcac
    17581 taccctttat cgacaataaa accaccatgg cagccgattc cagtagtacc gtaatggaat
    17641 tgctcaaatt gttacaggac cgtagtgacc tgacgttgct aaccaactcg gcggaagcga
    17701 ttcatgttct ggctcagtca gaaattaaag tcgtttcaac gggtggggaa ctaaacaaaa
    17761 atacactttc cctgcaagga agaattacta aagagatcat cagccgctac catgtcgata
    17821 tcatggtaat gagttgtaaa ggtcttgata ttaacagcgg cgcgctggac tctaatgaag
    17881 cggaagctga aatcaaaaag acaatgatcc gtcaggcgac agaagttgcg ttattggttg
    17941 atcactctaa gtttgatcgt aaagcttttg tccagttagc tgactttagt catattaatt
    18001 atataataac tgataaatca ccgggtgcag agtggatagc attttgcaaa gacaataata
    18061 tccagctggt gtggtaattt aagcacacgt tgtcaatatt atttgttcct gcgcctggaa
    18121 ataaaatgat gataaagatg cggcaagtga atttgccgca attgctacct tttatagcaa
    18181 atccgaggca cccaatggaa caatatgatc aaattggcgc aagactggac cgcttgcctt
    18241 tggcccggtt tcattatcgt atatttggta taataagctt tagtctgtta ttaacggggt
    18301 ttttgagtta ctcaggtaat gtcgtcttag caaagctggt aagcaatgga tggtcaaata
    18361 atttcctcaa tgccgccttt acctcggcat taatgtttgg ttatttcatc ggctcactta
    18421 ctggtgggtt tattggtgac tactttgggc ggcgcagggc gtttcgcata aatcttctca
    18481 tcgtcggtat tgctgcaaca ggggccgctt ttgtccctga tatgtactgg ctcattttct
    18541 tgcgcttcct gatgggaaca ggaatggggg cgctgattat ggttggctat gcctcattta
    18601 cggagtttat ccccgcgacg gtgcgtggaa aatggtccgc gcggctctca tttgttggta
    18661 actggtcgcc catgctgtct gcggcgatag gcgtggtggt tatcgctttt tttagttggc
    18721 gaataatgtt tcttctgggt ggtattggca tactgttagc ctggtttctc tcaggtaaat
    18781 actttatcga gtcgccacga tggctggcag ggaaagggca aatcgccggt gcagaaagcc
    18841 aacttcgtga agtagagcag caaattgaaa gggagaagag tattcgttta ccccagctta
    18901 ctttgaacca gagcaacagc aaggttaagg taatcaaggg taccttcagg ctcctgttta
    18961 aaggggaaat gttacgacgt acattagttg cgataactgt tttaattgca atgaacattt
    19021 cgctttatac cattaccgta tggataccga ccatatttgt taactccggc attgatgtcg
    19081 ataaatcaat attaatgacc gctgttatta tgattggtgc tccggtagga atatttattg
    19141 cggcattaat tattgatcat tttcctcgtc gattatttgg ctccgcctta cttattatta
    19201 ttgccgtgtt aggctatatc tattcaagtc agactacaga gtgggcgatt ttaatctatg
    19261 gtctggtgat gatcttcttt ttatacatgt atgtttgctt cgcgtcggcg gtttatatcc
    19321 cggagctttg gccaacgcat ttacgcctgc gcggttcggg tttcgttaat gccgtcggac
    19381 ggatcgtcgc agttttcacg ccctatggcg ttgcggcatt attaacacat tatgggtcga
    19441 tcacggtgtt tatggtgctt ggtgtcatgt tagtgctctg tgcgctggtt ctctccattt
    19501 ttggcatcga aacgcggaag gtgtcgctgg aagagatttc tgaggtgaat taaatctttc
    19561 gttgatggca tcaattgcca gatgtaccac tgacgtgttt tatttggcct acaatgccag
    19621 tacagaattg taggccagcg taaaattacc cctgcgtctc ttcccagtct gccagcgtat
    19681 atagcgttgc cccagctgct gacatctcca taaacgcgtg tgcactgtcc tggggctgga
    19741 tattcacgcc acgacagcca tcggtaatca cgtttacctt ataacctaac tgtaacgcgt
    19801 ccagcacggt aaacttcacg caatagtcag tagccaggcc cataacgatc aattcatcga
    19861 tttcatgatc gcgcaaccag tcatcaagcg cggttttctg ccgacgtccg ttatcaaaaa
    19921 aggcactgta actgtcaact aaaggatttt cgcctttatg gaacaccgct gcgatctctt
    19981 tttgattcag taacggatgt aattgtgcgc cttcactgtt ctgcacacag tgttcaggcc
    20041 agaaagtttg ccgcaggccg tcgagttggc ctggcgtata aggctctacg ccgtgctggc
    20101 tggcaaagct gccgtgattc gccggatgcc agtcctggct ggcgataacc gcttctccgc
    20161 gcgactggca ccagtcaata aggcggttag caacctccac cgtactgtca ccttccggta
    20221 cggcgagcgc gccaccagca cagaaatcat tttgtaaatc gaccagtaac agggcgcgat
    20281 ggggcattgc agtctcctta atcatccagc gtcagttcgc cgcgcaggtt ttggctcatg
    20341 gccttgcgaa tggtttcagt atccagttcc tggctcagca ggtaatgcag tttggttagc
    20401 gtggcttcta cagtcatatc tgcaccgcca ataacgccgg catgggcgag gacgttaccg
    20461 gtggcataac cactcatatt cactttaccg gacatacatt gtgtcaggtt gaccaccaca
    20521 ataccgcgat cgctggcttc ttgtaattcc tgcaggaagg ctttgttttg tggcgcatta
    20581 cccacgccat aggagcgcag aatcaatgct ttcaccggtt ggcgcagaaa attgcgcacg
    20641 acgtcagcag aaatccctgg ataaatcgtc actacgccaa ttggttgtgg cgtgattgga
    20701 tgaacgatta attccccttc accgtgcggg gcgggtggcg tattcaaacg acgaatatgg
    20761 ataccagctt ccagtaacgg aggaaggttt ggagaggcaa acgcatcaaa accatcggca
    20821 tgggctttgg tagtgcggtt gccgcgatac aatcggttat tgaaaaagag cgttacttcg
    20881 ttgatcggat aattcgccgc aacgtacaac gcattcagca gattaatttg tccgtcagag
    20941 cgtaactcag ccagcgggat ttgtgaccct gtcacaatga ccggtttacc gagattctcg
    21001 agcatgaacg acagcgcaga ggcggtatac gccatcgtgt cggtaccgtg tagaatgaca
    21061 aaaccatcat agtcgtcata gtgcgcttta atatcttcag caatatgctg ccagtcttcc
    21121 ggcgtcatat cagaagaatc catcaacggc gtatattcat gaatggtgaa atctggcatc
    21181 tccaggcgat ggaattccgg catcagcgcc agttggcgtt gtagatgacc tgacaccgga
    21241 atataaccct gctcggaacg ctgcatcccg atggtcccgc ccgtgtaggc gacgtaaatt
    21301 gatttctttt gcatgatatt gatatgaatg atatcgaaag agcaaaagta tatacgtgag
    21361 gcaggaggag cgcacctgat actatgcgga taaggaaata tggggcgaga cagcgggaag
    21421 aggattcgtc gaggtaaaaa ataatgtggc cgacgatctg tcggccactc agtacaagac
    21481 ttaacgcacg ttggcgcagg tcaggcaaaa cgcataacgg ttttgtgggt cgttaaacgc
    21541 ggccagcttg tcgctttcac ttttaacggt agaggctacc gaggcaagcg gtgcaggtaa
    21601 catggcctgg aacgcatctg gcaacattgc ccggacagaa ccagacatgt tgtccatcac
    21661 tttgtcgaag aaggtcggtt catcaacgta gtattccaga tgccactgtt tcacttttgc
    21721 gagctctgct gctttggcaa ccgcatcatc gaaatccccg agactatcga ccagcccgtt
    21781 agcttttgca tcctgaccgg tccagacgtg gccctgggcg attttatcaa tttgctccgg
    21841 cgtcgaatga cgcgcatcag caaccagcgt gataaagcgt ttatagccat tctcaatgct
    21901 taattgcatc atctgctgcg cttccggcgg cagtgccctg gtgatagaaa catccgccag
    21961 cggtgaagtt gagacaccat cagtatgaac accaatcgaa tccagactat tttctacggt
    22021 ggtgatcacg ccgaagatac cgatagaacc ggtcagggtg ctggggttag ccacaatgta
    22081 attagctggc gtggaaatcc agtaaccacc agatgccgcc atgccgccca tcgatacaac
    22141 cacagactta cccgctgccc gggctgctgc cagttcagcg cgaatcactt cagacgcggt
    22201 aacgctgccg cctgggctat taacacgcag gacaatcgct ttcactttcg ggtcaaggcg
    22261 agcgtcgcgg atttgtgccg cagtggtatc accgccaaca ttcccctgag tttcctcgcc
    22321 atccataatt gcgccattag cgaagacgac accgatgctg tcaccggtat ctgccggcgt
    22381 tttcaatgcg taatcgtaat aactgatggc gcgataattt ttatcagtct tactccagcc
    22441 gaactctttg gtcagagctt tttcgatttc ggcactcgat gccagtgcat cgaccagctt
    22501 gttttccagt gcatatttcg cggtatcgcc accggttttg gttaaaccct caagcaaccc
    22561 ttgcgcgcca gggaatacct gctgagcagg gatctgccgg ttagcggcaa cagtattcag
    22621 atagttttgc cacagctcac caatccagcg gctgtcagct tcgcgggctg ccggtgacat
    22681 atcatcacga ataaacggtt caacggcaga tttatacgta cccacgcgga acacatgggt
    22741 ggaaactttc agcttatcca gcaacgattt gtagtacaga ccgttggtag caaaaccgtg
    22801 cagatcaacc acgccttgcg gtgacagcca aattttattg gcgaaactgg cgagataata
    22861 ttgcccctgg ctgtagttct cgccaacggc ataaaccggt ttcccgctgt cacgaaactc
    22921 tttcagggct ttgccgatgt actgcataga cggctggtcg ccgcctgcga agtttttcag
    22981 atccatcacg ataccggtga tattgcggtc gtccttcgcc tggcgaatgg tgttgacgat
    23041 atcaaacagt gagttttcct gcagacgatc ggaactggca ccaagcagct ggcggcttaa
    23101 tttactaaac cgctgagaac tgtctggttt atcgacgatc acaccagaaa tgtccagcag
    23161 cagtgcgcca cgactggccg tttctttcga atcaccacca ctgacctgca tccagatccc
    23221 cacaccaacc agtacgagga aaataaagaa caggttaagt accatttcac ggacgaaatt
    23281 cagcagacgc cacgtccatt taaaaaatcc ggcaataaat cgccaaaggg ttcgcatgta
    23341 ttctcccaac ttaaggtcac acctgtcaca ggcgcaatag atgtggttat cgtaatgacc
    23401 caaccggcac ttgtcagcag gaattgcgga ctgtgctgta acaaaatcca ctctcgtgtt
    23461 aattttgtga ataaatatca cgacaggagt taatcaaatg gatgcactcg aactattgat
    23521 caatcgccgt agcgcctccc gcttggctga acccgcgcca acgggtgaac aactgcaaaa
    23581 catcctgcgt gcgggtatgc gtgcgccgga ccataagtcc atgcaaccgt ggcatttttt
    23641 tgtgattgaa ggggaagggt gcgagcgttt cagcgccgta ctggaacagg gggcgattgc
    23701 tgccggtagt gatgataaag ctatcgacaa agcccgtaat gcgccgttcc gcgcaccgct
    23761 catcatcacg gtggtggcga aatgcaaaga aaatcataaa gtcccgcgat gggaacagga
    23821 aatgtctgcc ggatgcgcgg tcatggcgat gcaaatggca gccgttgccc agggatttgg
    23881 cggcatctgg cgcagtggcg cattaactga aagtccggta gtgcgtgaag cattcggttg
    23941 ccgtgagcag gataaaattg tcggttttct ctacctcggc acgccgcagt tgaaagcatc
    24001 tacgtcgatt aacgtcccgg acccgacgcc gtttgtaact tatttctgat aattgtcgcg
    24061 aaactgtctg gattgtgagc aaaggcgcag gaattcagac actctcactt atcacttcac
    24121 ggaatgaggg ttaccatacc cggattgcat tgacaggaga tgtccatgag cgagaactcg
    24181 attcgtttga cccaatacag ccacggagct ggttgcggct gtaaaatttc cccaaaagtg
    24241 ttggaaacca tcctgcacag tgagcaggcg aagtttgttg atccgaattt gcttgtgggt
    24301 aatgaaaccc gcgacgatgc ggcggtgtac gatctgggca atggcaccag cgttatcagt
    24361 accaccgact tctttatgcc gatcgttgat aatcctttcg attttggccg cattgcggcg
    24421 actaacgcca tcagcgatat cttcgcgatg gggggcaaac cgattatggc gattgcgatc
    24481 ctcggctggc cgattaacaa actttccccg gaaattgccc gcgaagtaac cgaaggtgga
    24541 cgctatgcat gccgtcaggc gggtattgcg ctggctggcg gtcactccat cgatgcgccg
    24601 gagccgattt ttggtctggc ggtaacgggg atcgtaccga ccgagcgggt gaagaaaaac
    24661 agtaccgcac aagccggatg caaactgttc ctgacgaaac cgctggggat cggcgttctt
    24721 accacggctg agaaaaaatc actgttgaaa ccagaacatc agggactggc gacggaagtg
    24781 atgtgccgga tgaacatcgc aggcgcgtcc tttgccaaca tcgaaggcgt aaaagcgatg
    24841 accgacgtta cgggctttgg tctgctgggc cacttgagcg aaatgtgtca gggggctggt
    24901 gtgcaggcac gcgtcgacta tgaagcgatc ccgaaactcc ccggtgttga agagtacatt
    24961 aagttgggcg cagtacctgg cggcactgaa cgtaactttg ccagctacgg tcatctgatg
    25021 ggtgaaatgc cgcgtgaagt gcgcgatctg ctgtgtgatc cgcaaacttc tggcggtttg
    25081 ctgctggcgg tcatgccgga agcagaaaat gaggttaaaa ctacagccgc cgagtttggc
    25141 attgaactga cggcgattgg cgaactggtg ccagcgcgcg gcggtcgtgc catggttgag
    25201 atccgttaat tcaatgcggt tgtttattgc cgaaaaaccg agtctggcgc gcgccattgc
    25261 tgatgtcctg cccaaaccgc accggaaagg cgatggcttt atcgagtgcg gtaatggtca
    25321 ggtggtgact tggtgtatcg gtcacctgct tgagcaggcg cagccagacg cctacgacag
    25381 ccgctatgcg cgctggaatc ttgcggattt gccgattgtc ccggaaaagt ggcaattaca
    25441 gccacgaccc tccgtgacca aacaacttaa cgtcatcaaa cgcttcctgc atgaagccag
    25501 cgaaatcgtt cacgccgggg acccggatcg tgaagggcaa ttgctggtgg atgaagtgct
    25561 ggactatctg caactggcac cggaaaagcg ccagcaggtg cagcgttgct tgataaacga
    25621 cctgaacccg caggcggttg agcgggcgat cgaccgtctt cgttccaaca gtgagtttgt
    25681 gccattgtgc gtttctgcgc tggcgcgagc gcgtgccgac tggctgtacg gcatcaatat
    25741 gacccgtgcg tataccattc tcggtcgcaa tgccggttat cagggcgtac tttccgtggg
    25801 acgcgtgcag acacccgtgc tcgggctggt ggtgcgccgc gatgaagaga ttgaaaactt
    25861 cgtggcgaaa gacttctttg aagtcaaagc acatatcgtg acacctgccg atgagcggtt
    25921 taccgctatc tggcaaccga gcgaagcgtg tgaaccgtac caggatgaag aagggcgctt
    25981 gttacatcgt ccactggcgg agcatgtggt taaccgcatt agtggtcaac cggctattgt
    26041 caccagctat aacgataaac gggaatcaga atccgcgccg ctgccgtttt cgctttcggc
    26101 gttgcagatt gaagcggcaa aacgcttcgg tctgagcgcg cagaacgtgc ttgatatctg
    26161 ccagaagctg tacgaaacgc acaagctaat cacttatccg cgttccgatt gtcgctattt
    26221 gccagaagaa cattttgccg gacgccacgc ggtgatgaat gccatcagcg ttcatgcacc
    26281 ggatctgttg ccgcagccag tggtagatcc agatatacgc aaccgctgtt gggatgacaa
    26341 aaaggtcgat gcgcaccacg ccatcatccc gaccgcacgg agttctgcga tcaacctgac
    26401 ggagaacgaa gcgaaggtct ataacctgat tgcccgtcaa tacctgatgc agttctgccc
    26461 ggatgcggtg ttccgcaagt gtgttatcga actggacatt gccaaaggca aatttgtcgc
    26521 taaagcccgt tttcttgctg aagcaggctg gcgcacgctg ttaggcagca aagagcgcga
    26581 tgaagaaaac gacggcacgc cattgcctgt ggtggcgaaa ggcgatgagt tgctgtgtga
    26641 aaaaggtgaa gtggtagagc ggcaaaccca gccgccgcgc cattttaccg atgcaacact
    26701 gctttcggcg atgaccggga tcgcgcgctt tgtgcaggac aaagatctga aaaaggtcct
    26761 tcgtgcgacc gatggtctgg ggacagaagc aacccgtgcc gggattattg aactgctgtt
    26821 caagcgtggt tttctgacca aaaaagggcg ctatatccac tccaccgacg ccggaaaagc
    26881 gttattccat tcgctgccag aaatggcgac gcgaccggac atgaccgcgc actgggaatc
    26941 ggtgctgacg caaatcagcg aaaagcagtg tcgctatcag gactttatgc agccgctggt
    27001 ggggacgcta tatcagctta ttgatcaagc caaacgtacg ccggtgcggc agtttcgcgg
    27061 cattgtggct ccgggcagtg gtggcagtgc tgataagaaa aaggctgcac cgcgtaaacg
    27121 tagtgcgaaa aaaagtccgc cagcagatga agccggaagc ggggcgatag cgtaagcgag
    27181 cgaatctttc gtgctattcg agtcatattc tgaaatatcc agcggatcaa gaaaattcgt
    27241 tggatatttt tttgcatgga taaaattatc gcctctaaag tatgtaataa cagggaatgt
    27301 gatgaaaaaa gttttattac aaaaccatcc tgggagcgag aagtattctt ttaatggctg
    27361 gggaatattt aatagtaatt ttgaacgggt gattaaagaa aataaggcca tgctgctttg
    27421 taagtggggg ttttatttaa catgtgttgt cgctgtaatg tttgtattcg cagcgataac
    27481 atccaacggt ttgaatgaaa gaggcctgat taccgcggga tgctcttttc tttatctatt
    27541 aattatgatg gggcttattg ttcgggccgg ttttaaagca aaaaaagaac aactacatta
    27601 ttatcaggct aaaggtattg agccgctcag tatcgagaag ttacaggcgc tacaattgat
    27661 cgcaccttat cgattctatc ataagcaatg gtctgaaacg ctagagttct ggccgcgaaa
    27721 gcctgaacct ggcaaagata ccttccaata tcatgtgctt ccttttgact cgatcgatat
    27781 catcagtaaa agacgcgagt ctttagagaa tcaattgggt atcgaagata gcgaaagtta
    27841 ttgtgcctta atggagcatt ttctttctgg cgaccatgga gccaatacct ttaaagcaaa
    27901 catggaggaa gccccagagc aggttatcgc cgtgttgaat aaatttgctg tttttccctc
    27961 agactatatc tctgattgcg ctaataatcg ctccggtaaa tcctcggcga agctaatatg
    28021 ggcggcggaa ttatcatgga tgatctcgat atcaagcaca gcttttcaaa acgggacaat
    28081 tgaagaagaa ctggcctggc attatataat gcttgcttct cgaaaggcgc acgagttgtt
    28141 cgaaagcgaa gaagattatc aaaaaaatag tctaatggga tttctttact ggcatatctg
    28201 ctgctatcgc agaaagttaa cggatgctga acttgaagcg tgttatcgtt acgacaagca
    28261 gttttgggag cactacagta agaaatgccg ttggcccata agaaatgttc cctggggagc
    28321 atcatccgtt aaatactcat aaacgcctga aattttgccg ggggcgctgc gcttgcccgg
    28381 cctacattag agcgtaactg caatgaattg tgcccatctg taggcctgat aagcgtagcg
    28441 ccatcaggca tttacaactt aaatcacacc ctgcgccagc atcgcatcgg caaccttcac
    28501 aaaaccggca atgttcgcgc cctgcacgta gttggtttgc tcaccttcac caccatgctc
    28561 aacacaggca tggtggatat ccagcatgat gtgatgcaaa cgtgcgtcaa ctttctcggc
    28621 tttccagccc aggcgcgcag cgttttgtgc catttccagg cccgacgttg ctacaccgcc
    28681 agcattagcc gctttacccg gtgcaaacag tacgcctgcc tgctggaaca gttcagtcgc
    28741 ttcgatggtg gtcggcatat ttgccccttc agcgacggct ttaacgccat tggcgataag
    28801 ctgatgcgcg gtgtcaacat ccagttcgtt ctgggtggcg caaggcaggg cgatatcaac
    28861 cggtacagac cacggctgtt ggccttcgag atagaccaga ccaaattctt tggcgtaatc
    28921 tgccactcga ccatcgcggc tggctttgat ttcgataaga cgtgccagtt tctctttcgt
    28981 gaatccgctt tcatcaacta cagtgccgct ggagtctgac gcagtgatca cacgagcacc
    29041 aaattccatc gctttttcga tagcgtactg ggcgacgttg ccggagccag aaacggaaac
    29101 gcgcatccct tcaaaaccca taccgtggcg ttttaacatt gcttctgtga aataaaccag
    29161 accgtagccg gtagcttccg ggcgaataag actaccgcca aatgaaaggc ccttaccggt
    29221 gaagacgcag gcggtattgt tggagagctt tttcatcatc cccgccataa agccgacttc
    29281 acgaccacca acgccgatat cacctgccgg aacgtcggta tccgcgccca ggtggcgata
    29341 cagttcagtc atcagcgcct ggcaaaaacg catcacttta ccttcgcttt ttcctttcgg
    29401 atcgaaatcg ctgccgcctt taccaccgcc catcggcagg gtagtcaggg catttttgaa
    29461 ggtttgttca aagccgagga atttgagaat ggaaaggtta actgacggat ggaagcgcat
    29521 accgcctttg tacgggccga tggcagagct gaactgcaca cgccatgcac ggttgacctg
    29581 tatctggttg cgatcatcaa cccataccac gcgaaactgg atcacgcgct ccggttcaac
    29641 cagacgctcc agtaatgaca tctggcgata ttttggattt tgttcaagaa aaggccagag
    29701 tgtggtcatt acttcacgaa cggcttgcgc gaactcggtt tgattcgggt cgcgcttttg
    29761 gacatggttg aggaatgact ccagagaata tgtctgatcc atagatataa aaaccttata
    29821 tattaatacg attgtgctta tgttgtttat gtcatgtgct tttgcagttt tcgactatac
    29881 catcaagaat gtgacggtaa gcaagaaaaa aatgacccag gaaagcaaat taataacgag
    29941 agtaatctca taaccaaatg cgataactca gacgctaaag ttttgccacc cggctaccgt
    30001 tatcagtagc agagacaata acgttaagga gttccctgtg agtcgagcat tgttcgccgt
    30061 agtattagca tttccattaa ttgcactggc taacccgcat tatcgtccgg acgtggaggt
    30121 aaacgtaccg ccagaagtgt tcagttcagg cgggcagtca gcgcagccct gtactcagtg
    30181 ctgcgtttat caggatcaaa attattctga aggggcggta attaaagccg aaggaatact
    30241 gctgcaatgc cagcgtgatg acaaaaccct tagcactaat ccgctgctct ggcggcgcgt
    30301 aaagccataa acgcctctaa taatggaatg tcagcagggg ccagcggata ttgcagcgcc
    30361 tcttcaggtg agcaccagac cagcgcctga tgttcatgtg cctgtaacgt cccgtggaag
    30421 tcgggtacgt gccaggcatg aagatggata atccggcccg aaacttctcg ctgatggctg
    30481 gcaacatact cacccacagt tgcttcgatg tccaattctt cgtttaactc acgcaccagc
    30541 gcctgctgct ggctttcatc cagctcgact ttacctccgg caaactccca taatcccgcc
    30601 tgatcgctct gggcggggcg ttgcgcgagt aaaattttgc catcacgttc aatgatggcg
    30661 gcaacaactt caatcatttt cataaattac acacgggcag ataagcgaaa aatgccataa
    30721 tatccgcact gttgctcaat cagaaaggct ttatatttaa ggagtgcctg tgctagaccg
    30781 ccatcttcat ccccggatta aaccgttgtt gcatcagtgc gtgcgggttc ttgataaatc
    30841 gggcattacg ccagatggtt taacattagt gggatttgcc atcggcgtgc tggctctgcc
    30901 gtttctggcg ctgggctggt atctggcggc gctagtcgtc attttgttga acaggctgct
    30961 tgatggtctg gacggtgcgc tggcgcggcg cagagggctt accgacgcgg gcggctttct
    31021 tgatatttct ctcgattttc tcttttacgc gctggtgccg tttggcttta ttcttgctgc
    31081 accggagcaa aatgcgctgg caggcggctg gttgttgttt gcgtttatcg gcacgggcag
    31141 cagttttctc gcctttgctg cactggcagc aaaacatcag attgataacc ccggttatgc
    31201 acataagtcg ttttactatc tggggggatt aaccgaaggc acagaaacga tcttactgtt
    31261 tgtgctggga tgtttattcc ctgcatggtt tgcgtggttt gcgtggtttg cgtggtttgc
    31321 gtggtttgcg tggtttgcgt ggtttgcgtg gtttgcgtgg tttgcgtgga tcttcggtgc
    31381 gttgtgctgg atgacgacct ttacgcgggt gtggagtggg tatctgacgc tgaagtcgct
    31441 ccagcgtcag taattgcaat gttatttgct actgcccggg ccgcgttcac cggttgcaac
    31501 cggatttttt ggatcgctgc tccattcgta ccagccgccg tcatacacgg aaacattctt
    31561 ccaacccatg gcgcgtgcgt acataaaggt ttcggacgcg cgccagccgg tgccgcagta
    31621 gaatgaaact tgctgatctg gtttgatatt ccatgctttc cacatagcgg taatatcatc
    31681 ggcgctacgc atggtgccat ccgggttatg gaaatcttcc atatgcgtcg agtcgctacc
    31741 agcgtgtccc caacgtgctc cggctatttc accttttggt ttaatatagc tgtaaccgct
    31801 ggtcgtaccg ataaattctg gccacgaacg aatgctcacc agcgatgcat cctggcgatg
    31861 cagcagtcca cgggcctgtt ccatatcgag catcaactgc ggttgtgccg ggatcttcac
    31921 gccgaaatct ggttccgctt tcactttcgg tggcgttcct cgctcaacag gcagtcccgc
    31981 gtcggaccag gtttgccagc cgccatccag cagacgcaca tctttcacgc ctgcgtagag
    32041 cataatttgc gccacacgcg ctccagcgta tacgtcacgc ccgtacagaa tgaccgtggt
    32101 gtcatggcga atgccgtgtt ttgccagcat cgcttttagt tgttcatcag aaactttgtt
    32161 ccacagcggt tcgctttcca cttcgttggt atcgatgtag tcagcgccag gaatatggct
    32221 gataaggtaa agcttaggag cgccccaggc cgcttcaatg actttccagc caccgacagg
    32281 tttcgccgta acctctttac cttgttgcag gtcatgcagc cattgcggat aaaccagctg
    32341 ctcaaagtgc ggcagttttt gtagacggga aggttcgctt agcgcgtcac tcagaatgga
    32401 gatatgcgta aaacctgctt tttgcagtcg cgttttgacg gcgtcgacat ctttgccatt
    32461 accgtacagc gccaccgggg catcggcttt caggttatgt tgcttgatcc acgcgttgag
    32521 ctgttcggtg ctcattttgt cgagccagct agcagagagg tttaaggcgg caggttcatg
    32581 accagaaggg ccatttaagg tttgtggcca gccgttataa aacgcgctgg ggcgagtatc
    32641 tatcgctttg ccattttgtt gctgaagttg gtcaagtgta agaggcttcg ccagttcagc
    32701 ggcccacgaa gaagcgcaag ccagccctaa agccattgcc agcgcggtca tttgagaaac
    32761 acgtttcatc gaaaatcccg acattatagt tttgagtgcg ccattctgaa ctgacgcatt
    32821 gagaaaaact ttgcgttatc gcagtttgtt gtaattttct gaccactgcg ccatatccag
    32881 aacagaacta tcagcaggaa catcctggag atcgtgcgtt acctgaacga cggggatcgc
    32941 cagggcgcga acttcgctga acacccactg gcgaaaatta tcgcgcagag ccacatcaag
    33001 acggctgaat ggctcatcca ggagtaacgc ttttggctgg gcgagaaggg cgcgtagtag
    33061 agcaacgcgc gctcgctgac cgccagacaa agtggcagga tcctgatgga aagctccgtc
    33121 aaggcctgaa cgctcaagtg catcattcac ggcattacgt cgggcattcc ccttaagtgt
    33181 cgccggtagc gccagcagta aattttgccc gacgctgaac tggtcaaata acagtgcatc
    33241 ctgaaaaaga atgccaatct gacgctgtgc ggtgggtagc atgtcaatcc gttgctcatt
    33301 gagccatagc tcacctgtac aagaaaactg tccggccagt gcaccaatca tccatgaaaa
    33361 cagagtggat tttccacagc cagatggccc cattaacgtg acgatgtcac ctttatcaac
    33421 cgtaaagtta acgtttgtca gcaagcggct ttctggtaaa cgtagcgaaa catttttcac
    33481 gcagagcatt agcggattcc ttgtctgaca taacctaccc attttgcgac taacgcggtc
    33541 agggcaaaaa taataagcgg taacaacagt tgccataaag cctgggcggc gagaataccg
    33601 ttgctgccgc cgctgcttaa tgccaccgcc tcagtggtga gcgtcggaaa acgccccgcg
    33661 cccagccaca gcgtcggcat atactgcgca atactgactg aaaatcccac cgcgaaggca
    33721 atcagcacag ggcgcaacat gagtgggcat ttcacgtaaa agaagatttt ggcccgcgac
    33781 cagcccagtg tttgcgcaat caaaattaac cgtgaatcaa tgcgctgcca ggcgggttgc
    33841 aggataaaca gcatccacgg cattacccac agcagatgcc cccagaccac cgccgtccag
    33901 cttccatcca ggttcagcca tagcgccagc gtgtactagc ccgccaccag tggcagagcg
    33961 ggcaatagaa tgggcaacca tagccacaac tggcgacgct gtgcccccca ttccagccac
    34021 agcagtaaca ggagcaaagc gatgaatgtt gctaccagcc ccattgtcag actgttgatg
    34081 agtgcttcac tattgatcgt cgactgatcc gcgaggatcg ccagcagaac cacacacagc
    34141 acaccggtta agggtaaaaa actcgccagc gtagtgcctg gtaataaagg tgtggcaggc
    34201 ttgcgaatgc catctacgcg gggaatagtg cgccgccagc tgcgccatag cagatagctc
    34261 agcaaaacgt aggcggcgag taacagcatc agcagcagac tggcgagtgc gcctttcgtt
    34321 tgttgatccg cgtcgccctg agttaaccac tgccagctaa ttactgccag cgtcggtgga
    34381 ttacctggcc caagaataat cgccacatcc acgaccgaaa gcgaccaggc aacaatcgcc
    34441 agcatcgcca ttgccagcga aggcgcgacg gagggcaaca gcagccagtt caggcattgc
    34501 cagcggctgt agcccagcga atccagcaca atgacctgct gcaacagccg tttttcgctc
    34561 aatactgcca ctaagatcca cagcagaaat gcgctttctt tcactgcaag ggtgaggccc
    34621 agaccgatcc aaatcggtcc attggcggag tgaaatacgg gaaatagtca tatagcagcc
    34681 ctccgtcagc aaagagcagc agggcgctgg tggcaaaagc cacatgggga atggcgagca
    34741 gccacggcag acgggcgcac atacgctgcc atttcggccc aggccatagc gccacaatca
    34801 ccagcagggc aatcaacaat gccccgaccg ccgcgatggt tgtcgacacc agcgttgcca
    34861 gtaatgcctg cggtaactgt ggatcggcaa ataacgcctg ccagcgtgtc aacgacaacg
    34921 caggcgatat cagcgtgaga gctgccggga tcagcggtgc ataaatcacc gccaccatcg
    34981 cccacagcaa aaagattaat gcgtaccgta acggcgtaac cattcttgtt ccagtgcatt
    35041 cacccatcct gcgtgcggtt cagccagtac cggcggcaga tcctgcggca ttcttgattg
    35101 caatatttcg cgctgcccgt caggcagttt ttgcggatcg agaacagaag gatcgcccca
    35161 gacaacggga tctgctttac gcagttgcgc atcgggtgag agcaggaaat tggcaactac
    35221 cttcgccgca gcactggcat tcgcgttggc tggaatggtg acgaaatgca cgttgccaat
    35281 catcccctcg cgaaagccaa aactgtaact gcttgccggc aaatcgccgc tggcgatttt
    35341 ttgctgcgca tgcgcggggt taaaggtcag cgacaggcgc aatgtgccgg ctttcagcag
    35401 agcatccatc cgcgcgggcg aaggcgggaa atcctttcct tcgcgccaca aatacggatg
    35461 cagtgcatca agatattgcc acaagggcgc agtgacacgg gcgaaagtcg catcgtccgg
    35521 cgcttctttt aatgcggcgg gatctggcgt cagcataatc agcaactgtt caagaaacgc
    35581 cgtgccggta aagtccggtg ggcgcggata agtaaccgtg ccgggattgg ctttagcaaa
    35641 ctccagtaag gcttgcggcg tttgtggtgg ctgtggcgta acatcgcggc gggcgataaa
    35701 cgtcagttgt gcgccgcccc agggcgattc cgcaccttgt gtcggcactg aaaaatcttc
    35761 ccgcaccggc agctgtgtgt cgacatagcg ccagttgggc agagtctccg cccagcccgt
    35821 ttgtaataaa ttggcctctt tta
//
LOCUS       DAJTXY010000026        35186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTXY010000026 DAJTXY010000000
VERSION     DAJTXY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.026"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..1047)
                     /gene="setB"
                     /locus_tag="OO566_001415"
     CDS             complement(<1..1047)
                     /gene="setB"
                     /locus_tag="OO566_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6604754.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EIHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTS"
     gene            1415..2545
                     /gene="fruB"
                     /locus_tag="OO566_001416"
     CDS             1415..2545
                     /gene="fruB"
                     /locus_tag="OO566_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6604755.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNCIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2545..3483
                     /gene="fruK"
                     /locus_tag="OO566_001417"
     CDS             2545..3483
                     /gene="fruK"
                     /locus_tag="OO566_001417"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6604756.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3500..5191
                     /gene="fruA"
                     /locus_tag="OO566_001418"
     CDS             3500..5191
                     /gene="fruA"
                     /locus_tag="OO566_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6604757.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5615..6556
                     /locus_tag="OO566_001419"
     CDS             5615..6556
                     /locus_tag="OO566_001419"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6604758.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYVDSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSASKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6544..7482
                     /gene="psuG"
                     /locus_tag="OO566_001420"
     CDS             6544..7482
                     /gene="psuG"
                     /locus_tag="OO566_001420"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6604759.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQIAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7576..8826
                     /locus_tag="OO566_001421"
     CDS             7576..8826
                     /locus_tag="OO566_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6604760.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8897..9595)
                     /gene="yeiL"
                     /locus_tag="OO566_001422"
     CDS             complement(8897..9595)
                     /gene="yeiL"
                     /locus_tag="OO566_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCR6604761.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            9726..10667
                     /gene="rihB"
                     /locus_tag="OO566_001423"
     CDS             9726..10667
                     /gene="rihB"
                     /locus_tag="OO566_001423"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6604762.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMAGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10767..12017
                     /locus_tag="OO566_001424"
     CDS             10767..12017
                     /locus_tag="OO566_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6604763.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLVLNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12073..13161)
                     /locus_tag="OO566_001425"
     CDS             complement(12073..13161)
                     /locus_tag="OO566_001425"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6604764.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLVDCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13164..14020)
                     /gene="nfo"
                     /locus_tag="OO566_001426"
                     /pseudo
     CDS             complement(13164..14020)
                     /gene="nfo"
                     /locus_tag="OO566_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
     gene            complement(14094..15143)
                     /locus_tag="OO566_001427"
     CDS             complement(14094..15143)
                     /locus_tag="OO566_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6604765.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15242..16123
                     /locus_tag="OO566_001428"
     CDS             15242..16123
                     /locus_tag="OO566_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6604766.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16329..17798
                     /gene="lysP"
                     /locus_tag="OO566_001429"
     CDS             16329..17798
                     /gene="lysP"
                     /locus_tag="OO566_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6604767.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18090..20081
                     /gene="cirA"
                     /locus_tag="OO566_001430"
                     /pseudo
     CDS             18090..20081
                     /gene="cirA"
                     /locus_tag="OO566_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20113..20949)
                     /gene="yeiG"
                     /locus_tag="OO566_001431"
     CDS             complement(20113..20949)
                     /gene="yeiG"
                     /locus_tag="OO566_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6604768.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21207..21875
                     /gene="folE"
                     /locus_tag="OO566_001432"
     CDS             21207..21875
                     /gene="folE"
                     /locus_tag="OO566_001432"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6604769.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21892..23049
                     /locus_tag="OO566_001433"
     CDS             21892..23049
                     /locus_tag="OO566_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6604770.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23191..24230
                     /gene="galS"
                     /locus_tag="OO566_001434"
                     /pseudo
     CDS             23191..24230
                     /gene="galS"
                     /locus_tag="OO566_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            24510..25508
                     /gene="mglB"
                     /locus_tag="OO566_001435"
     CDS             24510..25508
                     /gene="mglB"
                     /locus_tag="OO566_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6604771.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25569..27089
                     /gene="mglA"
                     /locus_tag="OO566_001436"
     CDS             25569..27089
                     /gene="mglA"
                     /locus_tag="OO566_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6604772.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27105..28115
                     /gene="mglC"
                     /locus_tag="OO566_001437"
     CDS             27105..28115
                     /gene="mglC"
                     /locus_tag="OO566_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6604773.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28186..29421)
                     /gene="preA"
                     /locus_tag="OO566_001438"
     CDS             complement(28186..29421)
                     /gene="preA"
                     /locus_tag="OO566_001438"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR6604774.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29415..30655)
                     /gene="preT"
                     /locus_tag="OO566_001439"
                     /pseudo
     CDS             complement(29415..30655)
                     /gene="preT"
                     /locus_tag="OO566_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(30849..31088)
                     /locus_tag="OO566_001440"
     CDS             complement(30849..31088)
                     /locus_tag="OO566_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6604775.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31091..31810)
                     /gene="sanA"
                     /locus_tag="OO566_001441"
     CDS             complement(31091..31810)
                     /gene="sanA"
                     /locus_tag="OO566_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6604776.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31960..32844)
                     /gene="cdd"
                     /locus_tag="OO566_001442"
     CDS             complement(31960..32844)
                     /gene="cdd"
                     /locus_tag="OO566_001442"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6604777.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32974..33669)
                     /locus_tag="OO566_001443"
     CDS             complement(32974..33669)
                     /locus_tag="OO566_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6604778.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33666..34064)
                     /locus_tag="OO566_001444"
     CDS             complement(33666..34064)
                     /locus_tag="OO566_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6604779.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34303..>35186
                     /gene="dusC"
                     /locus_tag="OO566_001445"
     CDS             34303..>35186
                     /gene="dusC"
                     /locus_tag="OO566_001445"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6604780.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8635 a         8591 c         9297 g         8663 t
ORIGIN      
        1 cgacgtgttg gtatagagcg tggtggctga acctgcctga ccgggcatca aatcctgaaa
       61 atagagcatc ccgatgccgc ccagaatgcc aataaaaata gcatttagca gctgcaagcc
      121 caacagaatg gcaggtgaat gcgccatcag cattcctgcg taaaaacaga cgccacccac
      181 ggcagcaacg cgcattaaga aacgcttacc cagacgtttg gcgaaatatc cggcaatcaa
      241 catcgtcggg atttccagcc cggcggcggt ccccatcatc acaccggcca gtttctcggg
      301 aagatgcagt tcgttgataa taaatagcgg catgttgatg atgtacaggc tgttcgagcc
      361 ccacatcaat gtacaaatga caaacagcag tagcgtatcg cgacggttac ggcgcggcgc
      421 ttcaaccgtg ccggtcgcca gcggaagctc tttttgcatc gacggtaaaa acagccacac
      481 catcacgccg caaacaataa acgctaccgc tgcgctcaga tacattaccg taaagctgaa
      541 acccatcgct aacgcataag ccagcggtgg gccaatgacc catgccagtg aaacctgagc
      601 gcgtaaaaaa gagctgaaca tcaccgcctc acgtccggtt ttgtcggcat gttcacgggc
      661 aagggcaaac atttgcgggt tagcggtcga gccaaagctg ctaagaaaga cgccaacgaa
      721 tagcaaaaca aagtagttgc gattccaggc aaaaagggtg caggccagca cgcctaacag
      781 gcagcaaaag acaatcagcg atttgcgatc gccgcgctta tcagagcgcc cagcgagaaa
      841 ctgacttacc agaatcccaa tgacagcgct gccggtaaag aagaatccca ccatcgccgg
      901 acgggcatgt atttcatcgg taaggaaaat actgagtgtc ggggtttgca gagcacccgc
      961 aataccggtg agaaaggcaa cgattaaaaa cgccgtcgag gtcaggtcaa acgatttcgc
     1021 gctggagact gcgggggagt tatgcatgct ggtcgatcac tggaagagga agacgcggag
     1081 tttacgctgc ttgtcagaaa ataaacagac agcgaaatca aaagcgccac gaataatcaa
     1141 aatgccattt cactgttgct gattggctgc tgacgctgct gaaatgaaat tgctgataac
     1201 ggattttccc atcagcaatt aggaaaaatg acaaaaaatt gtgcagcaca tcaaactttt
     1261 gctcataact ttacggcttt ccttgcgtgc tgaaacagaa gtattatgct ttcttgaaac
     1321 gtttcagcgc gatcttgtct ttaaccctaa gccaggttgg cgcttttttt ctcatagagg
     1381 ctgaatcgtt tcaattcagc aagagaggag aactatgttc cagttatccg tacaggacat
     1441 ccatccgggc gaaaaggccg gagacaaaga agaggcgatt cgccaggtcg ctgcggcgct
     1501 ggtgcaggcc ggtaatgtag cagaaggcta cgtcaatggc atgctggcgc gcgaacagca
     1561 aacctcaacg ttcctcggca attgtattgc tattccacac ggcactaccg acacccgcga
     1621 tcaggtgctg aaaaccggcg ttcaggtatt tcagttcccg gaaggcgtca cctggggtga
     1681 cggtcaggta gcgtacgtgg caatcggtat tgctgccagc tcggataaac atctgggcct
     1741 gctacgccag ctgacccacg tactgagcga tgattccgtt gctgaacaac tgaagtcagc
     1801 aacaacagca gaagaacttc gcgcattact gatgggcgaa aagcagagtg agcagctgaa
     1861 gctcgacaac gaaatgctga cgctggatat cgtcgccagc gatctgctga ctcttcaggc
     1921 gctgaacgct gcgcgtctga aagaggcgag ggcagttgac gccactttcg tcaccaaagc
     1981 catcaatgaa caaccgctga atctcggaca gggtatctgg ctgagcgata gcgccgaagg
     2041 caatctgcgt agtgcgattg cggtaagccg tgcggcaaat gcttttgatg tggacggcga
     2101 aacggcagcc atgctggtga gtgtggcgat gaatgacgat cagcccatcg cggttcttaa
     2161 gcgtctcgct gatttgttgc tcgacaataa agctgaccgc ttgctgaaag cggatgcggc
     2221 aacgttgctg gcgctgctga ccagcgatga tgcgccgacc gacgacgtgt taagcgcgga
     2281 gtttgtggtg cgcaatgaac acggcctgca tgctcgtcca ggtaccatgc tggtcaatac
     2341 cattaaacaa tttaacagtg atattaccgt gacaaacctt gatggcaccg gcaaaccggc
     2401 aaacggacgt agtctgatga aagttgtggc acttggcgtt aagaaaggtc atcgcctacg
     2461 ctttaccgcc cagggtgcag atgctgaaca ggcgctgaaa gccattggcg acgctatcgc
     2521 tgctggtctt ggggagggcg cataatgagc agacgtgttg ctacgatcac ccttaatccg
     2581 gcttatgacc ttgttggttt ctgcccggaa attgaacgcg gcgaagtgaa cctggtgaaa
     2641 accaccggtc tgcatgcggc gggtaaaggc atcaacgtgg ccaaagtatt aaaagacctg
     2701 ggaattgatg tcaccgttgg cggcttcctg ggtaaagaca atcaggatgg ttttcagcaa
     2761 ctgttcagcg agctgggcat tgccaaccgt ttccaggttg tacaggggcg cacccgaatt
     2821 aacgttaagc tgacggaaaa agacggcgaa gtgaccgact tcaacttctc gggttttgaa
     2881 gtcacccccg ccgactggga acgctttgtg actgattctc tgagctggct cggtcagttc
     2941 gatatggtct gtgtcagcgg aagcttaccg tcaggcgtca gcccggaagc gttcaccgac
     3001 tggatgactc gcctgcgtag tcagtgtcct tgcattatct ttgatagtag ccgtgaagcg
     3061 ttagtagcag gtttgaaagc ggcaccgtgg ctggtgaaac ctaaccgccg cgagttggaa
     3121 atctgggcag gccgtaaact gcctgaaatg aaagatgtga ttgaagctgc acatgcgcta
     3181 cgtgaacaag gtattgcgca tgttgttatt tcactgggtg ccgaaggcgc gctttgggtt
     3241 aatgcctccg gcgaatggat cgctaaacca ccgtcagtcg atgtcgtaag caccgttggc
     3301 gcaggggatt ctatggttgg tggcctgatt tatggcttgc tgatgcgtga atccagtgaa
     3361 cacacactgc gtctggcgac agctgttgca gccctggcgg taagtcaaag caatgtgggt
     3421 attaccgatc gtccgcagtt ggccgcaatg atggcgcgcg tcgacttaca accttttaac
     3481 tgacagcagg agaggcataa tgaaaacgct gctgattatt gacgctaatc tcggtcaggc
     3541 acgcgcctat atggcgaaga ccctgctggg cgcggcggcg cgaaaagcaa aactggaaat
     3601 catcgacaat ccgaacgacg cagaaatggc gattgttctc ggtgattcca tcccgaatga
     3661 cagcgcgctg aacggtaaaa atgtctggct gggtgatatt tcccgggcag ttgcgcaccc
     3721 tgagctgttc ctgagtgaag ccaaaggcca tgcgaaacct tacactgcgc cggtcactgc
     3781 gacagcaccg gttgccgcca gcggtccgaa acgcgtagtt gcggtgactg cttgcccgac
     3841 tggcgtagca cacaccttta tggcggctga agccattgaa accgaagcga aaaaacgtgg
     3901 ctggtgggtg aaagttgaaa cccgtggttc tgttggcgcg ggtaatgcaa tcactcccga
     3961 ggaagtagcc gcagcggatc tggtgattgt ggcggcagat atcgaagtgg atctggcgaa
     4021 atttgctggt aaaccgatgt atcgtacctc taccggtctg gcgctgaaga aaacctcgca
     4081 ggaactggat aaagcggttg ctgaagcaac gccgtatgaa ccggcgggca aagctcaaac
     4141 ggcgaccact gaaggtaaga aagagagtgc aggcgcttat cgtcacttgc taacgggcgt
     4201 ctcttacatg ctgccgatgg tcgttgctgg tggtctgtgt atcgcgcttt cttttgcttt
     4261 tggtatcgaa gcgtttaaag agccgggtac gttggcagcg gcgctgatgc agattggtgg
     4321 tggttcagcc tttgcgctga tggtgccggt actggcaggt tatattgcct tctccattgc
     4381 cgatcgtccg ggcctcacgc cgggtctgat tggcggtatg ctggcggtca gcaccggttc
     4441 tggcttcatt ggcggtatta ttgcgggctt cctggctggt tacattgcga agttaatcag
     4501 tacgcaactg aaactgccgc agagtatgga ggcgctgaaa ccgatcctga tcattccgct
     4561 aatttccagt ctggtggtcg gtctggcgat gatctacctg atcggtaaac cagttgctgg
     4621 cattctcgaa gggctgactc actggctgca gaccatgggg actgcgaatg cggttctgct
     4681 gggggcgatc ctcggtggca tgatgtgtac tgacatgggc ggtccggtaa acaaagcagc
     4741 gtacgcattc ggtgtgggtc tgctgagtac tcaaacctat ggcccgatgg cggcgattat
     4801 ggcggcaggt atggtgccac cgctggcaat gggcctggca acaatggtgg cgcgtcgcaa
     4861 attcgacaaa gcgcagcagg aaggtggcaa agccgctctg gtattggggc tgtgcttcat
     4921 ttcggaaggt gcaattccgt ttgctgctcg tgatccgatg cgtgtgctgc cgtgctgtat
     4981 cgtgggtggg gcgctgactg gcgcaatctc aatggcgatt ggtgcgaaac tgatggcacc
     5041 gcacggtggt ctgtttgttc tgctgatccc tggcgctatt acgccggtat tgggttacct
     5101 ggtagcaatt attgccggta cgctggtggc gggtttggcc tatgccttcc tgaaacgtcc
     5161 ggaagtggac gcagtagcga aagcagcgta ataaaaggtg tgttacaggg cagaaatttc
     5221 ctgccctgta acatttgatg agcgcaaaga gaatgaccag cttgtgaatg taattttatg
     5281 aatttaaatg gttttatctt tcagtagaaa cttcaaacgt tcatcaaaca ctaaggttat
     5341 ttcctcaagg gcagattatt ttctgccctt ttttagtgtt cctgttctgt gtatcccgtc
     5401 acaaattcct cgcttttgta attcaggcat ttaatcgcat tttgtgagcc tgctcaaatc
     5461 tgcccatttt gcctctctgg cggtaattta ctttacgtaa aacatgcata ctatgagcac
     5521 atgtttaaat atgaacacat gtttaaaccg tctttgcagt atctatattc ttgtggattg
     5581 acagggttgg taatttttta ccagaggacg tgctatgcgc gaaaaggatt atgtcgtaat
     5641 tataggttcg gcgaatattg atgtcgccgg atattcacat gaatcattaa attatgtgga
     5701 ttcaaatcca ggtaaaataa aatttacgcc tggtggagta gggcgcaata ttgcacaaaa
     5761 cctggcgttg ctgggtaaca aagcctggct actgagcgcc gtaggcagtg atttttatgg
     5821 tcaatcgctg ctaacgcaaa ccaatcaatc tggtgtttat gtcgataaat gcctgattgt
     5881 gccgggagaa aatacgtcga gttatttatc attactcgat aataccggtg aaatgctggt
     5941 tgctataaat gacatgaata ttagcaacgc tattacagct gaatatctcg cacagcaccg
     6001 tgaatttatt cagagggcaa aggtcattgt cgcggactgt aatatcagtg aagaggcact
     6061 ggcatggatt ctggataatg ccgccaacgt acccgtattt gtcgatccgg tttcagcgtc
     6121 gaaatgtgtc aaagtgcgcg accgtctaaa tcagatccac actctgaagc caaaccgcct
     6181 tgaagcggaa accctgagtg ggattgcgct gtcagggcgt gacgatgtgg caaaagttgc
     6241 tgcctggttc catcaacatg gcctgaaccg actggtattg agcatgggcg gcgacggcgt
     6301 ttattacagc gatatcagcg gtgaaaatgg ctggtctgcg ccgatcaaaa ccaatgttat
     6361 taatgttacc ggagcgggcg atgccatgat ggcgggactt gcttcgtgtt gggtagacgg
     6421 aatgccgttt gccgaatctg ttcgtttcgc acagggatgt tcgtcaatgg cgctctcctg
     6481 tgaatacacc aataaccccg atttatcgat tgccaacgtt atatcgttag tggagaacgc
     6541 agaatgtctg aattaaaaat ttcccctgaa ttattacaaa tttctccgga agtgcaggac
     6601 gctttaaaaa acaaaaaacc ggttgtggcg ctggaatcaa ccattatttc tcacgggatg
     6661 ccgttcccac aaaatgccca gatcgcaatt gaagtagaag aaactattcg taaacagggc
     6721 gcagtacctg ccactatcgc cattattggc ggcgtgatga aagtgggttt aagcaaagaa
     6781 aaaattgaat tactgggtcg tgaagggcat aacgtgacca aagttagtcg tcgcgattta
     6841 ccttttgttg ttgccgccgg aaaaaatggc gcaaccactg tggcttcaac gatgattatt
     6901 gcggcgcttg ccggaattaa agtatttgcc accgggggaa ttggtggtgt gcatcgcggg
     6961 gcggaacata ccttcgatat ttctgccgat ttgcaagaac tggcaaatac taatgtcacc
     7021 gttgtttgtg ccggggcgaa atctattctc gatttaggat taaccactga gtatttagaa
     7081 accttcggtg tgccgttaat tggctatcag actaaagcgc tgcctgcgtt tttctgccgt
     7141 accagctcgt ttgacgtcag cattcgtctc gacagcgcca gtgaaattgc ccgtgcaatg
     7201 gcggtgaaat ggcaaagcgg gctgaacggt ggcctcgtgg tagcgaaccc gatcccggaa
     7261 cagtttgcga tgccggagga atctatcaat gcagccatag atcaagccgt cgccgaagcc
     7321 gaagagcagg gcgttattgg taaagaaagt acaccgttcc tgctggcgcg cgttgctgag
     7381 ctgaccggcg gtgacagcct gaaatccaat atccagctgg tgttcaacaa cgccattctg
     7441 gcgagtgaaa ttgccaaaga ataccagcgt ctcgcgggtt aatttctgac gggcagaaag
     7501 ccgtcgcccg tactcatcaa gcataccaaa acctggcgct gacgccgggt ttactggcat
     7561 gaagggaaaa tcattatgga tataatgaga agtgttgtgg ggatggtggt gttactggca
     7621 atagcatttc tgttgtcagt gaataaaaag agcatcagtt tgcgcacggt tggagccgca
     7681 ctgctgctgc aaatcgctat tggtggcatc atgctctact tcccaccggg aaaatgggca
     7741 gtagaacagg cggcattagg cgttcataaa gtgatgtctt acagtgatgc cggtagcgcc
     7801 ttcatttttg gttcgctggt tgggccgaaa atggatgtcc tgtttgacgg tgcgggtttt
     7861 atcttcgcct ttcgcgtact tccggcgatt attttcgtta ctgcgctcat cagtctgctg
     7921 tactacattg gcgtgatggg gctgctgatt cgcatccttg gcagcatttt ccagaaagcc
     7981 ctcaacatca gcaaaatcga atcttttgtt gcggttacca ctattttcct cgggcaaaat
     8041 gagatcccgg cgatcgttaa accgtttatc gatcgcgtga atcgcaacga gttgtttacc
     8101 gcaatttgta gcgggatggc gtccattgct ggttcgatga tgattggtta tgccggaatg
     8161 ggcgtaccaa ttgactacct gttagcggca tcgctgatgg cgatccccgg cggtattttg
     8221 tttgcacgta ttcttagccc ggcaaccgag ccttcgcagg tcacatttga aaatctgtcg
     8281 ttcagcgaaa cgccgccaaa aagctttatc gaagcggcgg cgagcggtgc gatgaccggg
     8341 ctgaaaatcg ccgctggtgt agcgacggtg gtaatggcgt ttgtcgcaat tattgcgctg
     8401 atcaacggca ttatcggcgg aattggcggc tggtttggtt tcgccaatgc ctctctggaa
     8461 agtatttttg gctatgtgct ggcaccgctg gcgtggatca tgggtgtgga ctggagtgat
     8521 gccaaccttg ctggtagcct gattgggcag aaactggcga ttaacgaatt catcgcttac
     8581 ctgagtttct ccccatacct gcaaacgggc ggcacgctgg aagtgaaaac cgtggcgatt
     8641 atatcctttg cgctttgtgg ttttgctaac tttggttcta tcggtgttgt cgttggcgca
     8701 ttttcggcta tttcgccaaa acgtgctcca gaaatcgccc agcttggttt acgggcgctg
     8761 gcagcagcaa cgctttccaa cctgatgagt gcgactattg ccgggttctt tattggtctg
     8821 gcgtaatgct cgatttgtag accggataaa acgtattaag cgttgcatcc ggcaatcgcg
     8881 ctattgcaaa taatttttac tgcatcatcc cggagaattt attctccggg tccatctcca
     8941 gcgccagtcc tgacaactgc tttctgtttt taattagata tcctttcttg cttttaatta
     9001 ataaaccgtc gtgaatgaat tgcgcgagaa catataaaag atgtcgataa gaaacgccta
     9061 aatactctgc cgcttgcgta tgcttttcgt gataaagatc accttcttgt gagagtaata
     9121 taaatgctgc caggcgattc attaacggaa atgattgatt ctgagttaaa gaaacaatat
     9181 tacgataatt tttatgactt aaggtgacgc aaagttttcg taaaaatagg gtgtcgttta
     9241 ataacagcgg acggtaatgt ttcataggga gcgcaaggca ccaacactct tcaatagcct
     9301 gcaccgcacg cggttcatgg tctttgtcga ttaactcaat ctcgccaata aaacagggag
     9361 cagcaaagaa atcgatcagc gacacgcgac cattagcaag cgtggcgtaa agcctggcgc
     9421 ggcctcgcgt cagataaaac agccatgacg gctgttgccc ttcctgcaca atgtagtcac
     9481 gcgctaaaaa atgaaacagc cgcgtatcgg ctgaaacatc cgttgaaaag caatccttaa
     9541 acgcagattc actcatcaat tgctgcttaa ggtcattatt gtggatttct ttcacgatgt
     9601 ttatccgcta tgagatttct catatttagc atacgtacgc tgttgttaag attcaatcat
     9661 cagcccggtc gaattttctt tgctgtagaa aaaaataacg caactggaaa cagaggaaat
     9721 aaacaatgga aaagagaaaa attattctgg attgtgagcc gggtcatgat gatgctattg
     9781 caatgatgat ggcagcgaaa catccggcaa tagatttatt aggcatcact attgtagcgg
     9841 gtaatcagac gcttgataaa acattaatta atggcctgaa tgtttgccag aaactggaga
     9901 ttaatgttcc ggtttatgcg gggatgccgc aacccattat gcgtaaacaa atcgttgccg
     9961 ataatattca cggtgaaacc ggactggatg gcccggtatt cgagccgctg acccgccagg
    10021 cagaaagtac ccatgcggtg aaatatatca tcgataccct gatggcaagc gatggcgata
    10081 tcactctggt gccggttggt ccgctttcaa atatcgcggt ggcaatgcgt atgcaacccg
    10141 caatcctgcc caaaatccgt gaaattgtgc taatggccgg cgcttacggt acaggcaact
    10201 tcaccccatc tgccgagttc aatatctttg ccgacccgga agccgcccgc gtggtgttca
    10261 cctccggcgt tccattagtg atgatgggcc tcgatcttac caaccagacc gtttgcaccc
    10321 cggatgtaat tgctcggatg gaaagggtag gcggccctgc cggagagctg ttcagcgaca
    10381 tcatgaactt cactctcaaa acgcagttcg aatactacgg cctggctggc ggcccggtgc
    10441 atgacgccac ctgcattggt tatctgatca accctgatgg cattaaaacc caggatatgt
    10501 acgtcgaagt ggacgtcaac agcggccctt gctacgggcg tacggtctgc gacgaactgg
    10561 gcgttcttgg caagcccgct aataccaaag tcggcatcac tattgatacc gactggttct
    10621 ggggcttagt cgaagagtgc gtgcgcggct acatcaaaac ccattaagtg ttgataacaa
    10681 gccgggacag tatcgcaaga tgcaggcccg gctatttctg atgggcagaa agccgtcgcc
    10741 caaaatgaca tgaagagata atcactatgg atgtcatgag aagtgttctg ggaatggtgg
    10801 tactgctggc aattgcgttt ttactgtcag taaacaagaa gaagatcagc ctgcgtaccg
    10861 ttggcgcggc gttagtgtta caggtcgtga ttggcgggat tatgctttgg ttaccgccag
    10921 ggcgttgggt cgcagaaaaa gtcgcttttg gcgtccacaa agtgatggcg tacagcgacg
    10981 caggtagcgc atttatcttc ggttctctgg tcggaccgaa aatggatacc ttatttgatg
    11041 gcgcaggatt tatctttggt ttcagggtat taccggcaat tatcttcgtc actgcactgg
    11101 tgagtattct ctactacatc ggtgtgatgg ggatcttaat tcgcattctc ggcggtatct
    11161 tccagaaagc tttaaatatc agcaagatcg agtcattcgt cgcggtcacc accattttcc
    11221 tcgtgctaaa cgaaattccg gcgatcgtca aaccctttat cgatcgtctg aatcgcaatg
    11281 aattatttac agcgatttgt agtggcatgg cctcgattgc tggttcgaca atgattggtt
    11341 acgccgcact gggcgtacct gtggaatatt tgctggcggc atcgttaatg gcgatccctg
    11401 gcgggatctt gtttgcccgc ctgttaagcc cggctacgga atcttcgcag gtttctttta
    11461 ataacctctc tttcaccgaa acaccgccaa aaagcattat tgaagccgat gcgacagggg
    11521 caatgaccgg gctgaaaatc gccgccggtg tagcgacagt tgttatggca tttgtcgcca
    11581 tcattgcgtt aattaacggt attatcggcg gcgttggcgg ctggtttggt tttgaacatg
    11641 cctcgctgga gtccatttta ggttacctgc tggccccact tgcgtgggta atgggtgttg
    11701 actggagtga tgcaaatctt gccgggagtt tgattggaca gaaactggca ataaatgaat
    11761 ttgtcgctta tctcaatttc tcaccctatc tgcaaacggc tggcactctg gatgctaaaa
    11821 ccgtggcgat tatttccttc gcgttgtgcg gtttcgctaa ctttggttct atcggggtgg
    11881 tggtgggggc gttttctgcg gttgcgccac accgtgcgcc ggaaatcgcc cagcttggtt
    11941 tacgcgcgct ggcggcggcg acactttcta atctgatgag tgccaccatt gccgggttct
    12001 ttattggttt agcgtagtgt gcaatttatg agccggatta gatgcggtca agcgtcgaat
    12061 ccggcaacat tgttacacca ttggcactaa tgacagcgcg ttatcggcag acagggtagg
    12121 gttgtttaag ctgccgctgg cgcgtgaaat tgccgcgcag gccatcgcaa aacgggcgct
    12181 gtcacggaaa ttgtttccct ccagaaagct atataccaga cccgccataa aaccatcgtc
    12241 agcaccaaaa ctgtctaccg tcgtatgcgc tggcacagtc agcaaaaatt gttcaccatc
    12301 cttttcgctg caataaactg actcatcggg caaataaaca aacagttgct gaacaccttg
    12361 ctgatgcaat gcattcactg cggcattacg gtcagcatcg ctggtgatcg cctgtcccca
    12421 taaaatttcc agctccggta aagtgggttt cagggtgtga atatgcgcca gccagtgttt
    12481 gattttgccc gctttgaatt ctgaaacggt atcgacaaac accgggatct catcagcgag
    12541 ggtaaagacc cattccagcg cctcggctgt caggttgcaa tcaaccagta ccacgcccgc
    12601 atgacgaagt aaatcgcgcg acccgttcag tagttgcggt gtcaactgtt ccagcagatg
    12661 ggtatcgtta atcgccagca cggtttgatc gtctcgattg gcaattgcca gatacgtcga
    12721 tgtgctttga ccatgcaaac gaacgcagcc ggagacattc acgccggcac ggcgcgtttc
    12781 ttccaggagc atttcgccat aaaagtcatc gccaatcact gaaagcaaat ggacgtcacg
    12841 gcctaacagc gccagattgt gggcgatatt gcgtccaacg ccgcctgccg aacaatgaat
    12901 tgtaccggga tgagaagccg cttgcgggta atggatatcc gccatcccgc gaatatccat
    12961 attgattgtc cccaccacta cgcagtattc ctgctcggtg agaatgtaac ctttgccttt
    13021 aatccggcct ttgcgcatta aatccataat atgcgccgca acgcgcgaac gactgatttg
    13081 cagcatgtcc gcaatttcgt tctgctgaat cagcgggtta cgccgtaaaa ttgcaaggat
    13141 ctccttttcc cggttattca tcttcaggct accgcttttt cagtttgttg cgctttcagc
    13201 caggcgatct cttctgccca gatatccggg ttgatggttt caaggatcag cggaatgccg
    13261 tcgaaacggt cgttctgcat aatccagcgg aacgcatcat gaccgatatt gccttcaccg
    13321 aggctatgat ggcggtcaac gcggctgcca aaggtacttt tcgcatcgtt aaggtgcatc
    13381 ccgcgcagat acttaaagcc gacaatacgg gcaaaatcag cgaacgtttt ttcgcattcg
    13441 gctggagtac gcaaatcata cccggcagcg aaagcatggc aggtatcaat gcagacgccg
    13501 acgcgggatt tatcttccac gccgtcgata atcgccgcga gatgttcgaa tttaaaccct
    13561 aagttactgc cctgaccggc ggtgttttca atcaccgctg ttacaccttg agttttatcc
    13621 agcgcaatgt tgatggattc ggcaatacgc gcaaggcaat cctcttctga aatctgcatc
    13681 agatggctgc cagggtggaa gttgagcaaa gaaagcccca gtgttcgcaa cgctgcattt
    13741 catctataaa ggcatcgcgc gatttttcca gagcttcagt gaccggatgt ccgaggttga
    13801 tcagataact gtcgtgggga agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg
    13861 ctttgaattc atcgatggtt tgcgtcgtga gcggtgcggc acgccactga cgttggtttt
    13921 tggtgaacaa ggcaaacgcg gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca
    13981 gaccgccagc agcactaacg tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc
    14041 cgctggagga aaacggtaat gatagcgggt taacaggagc aagaagtagt ggtttatgcg
    14101 atgacgcttt gaatcacata gttaatcgca ccaccaccaa caatcagcca ggcaaacagt
    14161 accagtgcca tcagcagagg tttcgcccca gcttttttca gcgcgctgac gtgagtggtc
    14221 agacccagcg ccgccatcgc cattgccagc aggaaggtat ccagcgttac cagcatgttc
    14281 accacgctct gcggtaacag gtggaacgag ttaaagatgg caactacgat gaacaagatg
    14341 gcaaaccacg gaatagtgat tttgcttttc tcgccgctgt tcgccccaga cagctgttta
    14401 acacgcgccg ccagcaggat gaggaacgga gccagcatca tcacgcgcag cattttggaa
    14461 ataactgctg cgttttctgc atccgggctg atggcatgac ctgccgccac cacctgcgcc
    14521 acttcgtgca cagtagaacc aatgtagata ccgaaagttt ccggactaaa ccattgagac
    14581 atcagcggat atatcgccgg gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt
    14641 gcaacagcca cggttacttt actggcttcc gctttcacta ccggctcagt cgccagtacc
    14701 gcggcagcac cacagatact gctaccggca ccgatcaacc aactggtgtg cttatccaga
    14761 ccaaacactt tctgccccag gaagcaagcc agcaggaagg tactggacag cgtcagcacg
    14821 tcaatgatga tcccactgat accgacatcg gcaatttgcg agaacgtcag acggaagcca
    14881 taaagaatga tacccagacg taataaatat tgcttggcaa acagcacacc accgtcacag
    14941 cttttccaga tgtgcggata gatggtgttg cctaaaacca tccccaacaa gattgcgagg
    15001 gtgagggcac taaacccggc acccgcaacc gcgggaatgg aaccacccca cagggcgacc
    15061 ccggtgataa ctgcactcag ggctaacccc ggaataaaat gccacagtgt acgatgttgt
    15121 ttctgtaagg tgatattcgt cataaccctc tcctttaacc gggcataagg ttacgactaa
    15181 ctggtttaaa aataaaattg attatatatt tataattaat ctttataagt ggtaagcgac
    15241 tatgcacatc accctccggc agttggaagt ttttgcagaa gtattgaaaa gtggatcaac
    15301 cacccaggcg tcggtgatgc tggcgttgtc gcaatcagca gtgagcgcag ccttgactga
    15361 cctggaaggg cagcttggcg ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa
    15421 tgaacacggg cggctgctct atccgcgagc gttggcactg cttgaacagg cggttgaaat
    15481 cgaacaactg tttcgcgaag acaacggcgc gattcgtatc tatgccagta gtaccatcgg
    15541 taactacatt ctgcctgcag ttatcgcccg ttatcgccat gattatccgc agttgccgat
    15601 tgaacttagc gttgggaaca gccaggacgt gatgcaagcg gtgctggatt tccgcgttga
    15661 tattggcttt attgaaggac cgtgccacag cactgaaatc atttctgaac cgtggctgga
    15721 agacgagctg gtggttttcg ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt
    15781 agaacagctg gccgctgcgc cgtggatcct gcgtgaacgc ggttccggca cgcgggagat
    15841 tgtcgattat ctgttgctgt cacatttacc gaagtttgag atggcgatgg aattaggtaa
    15901 ctccgaggca atcaaacatg cggtgcgtca tgggttggga attagttgcc tgtcgcgacg
    15961 tgtgattgaa gatcaattgc aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg
    16021 cctgatgcgt acgttgtggc gtatacatca tcggcaaaaa cacctttcca acgcgctacg
    16081 gcgctttctg gactattgcg atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca
    16141 tgctggtgct gtgtcgattt cgtgacgcag cgccttcagc atgcattcgc cagaaaagag
    16201 attggctgct ttacttataa tccctgggcg atcatgaagg tgtcttataa ccgtgtattt
    16261 ctgccggaag gattgccaat cgtctgctac aatcgcgcca tcatttttaa gatggatagc
    16321 atttttgtat gggttccgaa actaaaacta cagaagcgcc gggcttacgc cgtgaattaa
    16381 aggcgcgtca cctgacgatg attgccattg gcggttccat cggtacaggt ctttttgttg
    16441 cctctggcgc aacgatttct caggcaggtc cgggcggggc attgctctcg tatatgctga
    16501 ttggcctgat ggtttacttc ctgatgacca gtctcggtga attggctgca tatatgccgg
    16561 tttccggttc gtttgccact tacggtcaga actatgttga agaaggcttt ggcttcgcgc
    16621 tgggctggaa ctactggtac aactgggcgg tgactattgc cgttgacctg gttgcagcac
    16681 agctggtcat gagctggtgg ttcccggata caccgggctg gatctggagt gcgttgttcc
    16741 tcggcgttat cttcctgttg aactacatct ctgttcgcgg ctttggtgaa gcggaatact
    16801 ggttctcact gatcaaagtc acgacagtta ttgtctttat catcgttggc gtgctgatga
    16861 ttatcggtat cttcaaaggc gcgcagcctg cgggctggag caactggaca atcggcgaag
    16921 cgccgtttgc tggtggtttt gcggcgatga tcggcgtagc tatgattgtc ggcttctctt
    16981 tccagggaac cgagctgatc ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca
    17041 ttccacgcgc ggtacgtcag gtattctggc gaatcctgtt gttctatgtg ttagcgatcc
    17101 tgattatcag cctgattatt ccgtataccg atccgagcct gctgcgtaac gatgttaaag
    17161 acatcagcgt cagtccgttc accctggtgt tccagcacgc gggtctgctc tctgcggcgg
    17221 cggtgatgaa cgcagttatt ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg
    17281 cgtctactcg tatgctgtac accctggcgt gtgacggtaa agcgccgcgc attttcgcta
    17341 aattgtcgcg tggtggcgtg ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt
    17401 tgtgcttcct gacctccatg tttggcaacc agacggtata cctgtggctg ctgaacacct
    17461 ccgggatgac gggttttatc gcctggctgg ggattgccat tagccactat cgcttccgtc
    17521 gcggttacgt attgcaggga cacgacatta acgatctgcc gtaccgttca ggtttcttcc
    17581 cactggggcc gatcttcgca ttcattctgt gtctgattat cactttgggc cagaactacg
    17641 aagcgttcct gaaagatacc attgactggg gcggcgtagc ggcaacgtat attggtatcc
    17701 cgctgttcct gattatttgg ttcggctaca agctgattaa aggaactcac ttcgtacgct
    17761 acagcgaaat gaagttcccg cagaacgata agaaataagt tttctccctt ccttgctaag
    17821 ccctctcaac cgagagggct ttttcaattc catttccctg acaaatcatg cggatataaa
    17881 atttaacatt tggattgata attgttatcg tttgcattat cgttacgacg caatcaaaaa
    17941 aggctgacaa atcagaggct gttccggctt tctgggatga tcacctgcat acaaataagt
    18001 ccaccgcgat gctgccgtac gcaaggggac gtaaagaaga tgtgagcgat acccatttta
    18061 ttttcgtagt tacctcatgg agatatggaa tgtttaggtt gaaccctttc gtacgggtcg
    18121 ggctgtgttt gtccgctatt tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg
    18181 aaacgatggt tgtcactgca tcttccgtgg aacaaaatct taaagatgca cccgccagta
    18241 tcagcgtcat tacccaggaa gacctgcagc gaaaaccggt acagaatctg aaggatgtcc
    18301 tcaaagaagt gcctggcgta caactgacga acgaagggga taaccgtaag ggcgttagta
    18361 ttcgtggtct ggacagcagc tacaccctga ttcttgtcga cggtaaacgc gttaactccc
    18421 gcaatgccgt cttccgccac aatgatttcg atctgaactg gatcccggtc gattccatcg
    18481 aacgtattga agtggttcgt ggcccgatgt cgtcgctata cggctccgat gcgctcggcg
    18541 gtgtagtgaa tatcatcacc aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg
    18601 ataccaccat tcaggaacat cgcgatcgcg gtgataccta taacggtcag ttctttacca
    18661 gtggaccatt aattgatggt gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg
    18721 aaaaggatga cccgcaaaac tcaacgacca ccgataccgg agaaacgccg cgtattgaag
    18781 gattctccag ccgcgacggc aatgtcgaat ttgcctggac accgaatcaa aatcacgatt
    18841 ttactgccgg atacggtttc gaccgtcagg atcgtgattc cgactagctg gacaaaaacc
    18901 gcctggaacg ccagaactac tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg
    18961 aactgaaatc ctacggtgag aaagtcgaga acaaaaaccc tggcaacagc agcccgataa
    19021 cttccgaaag caatacggtc gacggcaaat acacgttgcc gctgacggcg attaatcagt
    19081 ttctcacggt tggcggtgaa tggcgtcacg acaaacttag cgatgcggtg aacctgaccg
    19141 ggggaaccag ctccaaaacg tctgccagcc agtacgcgct gtttgtggaa gatgaatggc
    19201 ggatcttcga gccgctggcg ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg
    19261 gtgaacactg gagtccgcgt gcctacctgg tttataacgc caccgacacc gtaacggtga
    19321 aagggggctg ggcgacggca tttaaagcac cttctctgtt gcaacttagc cctgactgga
    19381 cgagcaattc ctgccgtggc gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa
    19441 ccagcgaaag ttgggagctg gggctttact acatgggtga agaaggctgg ctggaagggg
    19501 gtgaatccag cgttatcgtt ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta
    19561 cgtctgacgt caacgctgca ccgggctacc aaaactttgt tggttttgag acgggcgcta
    19621 acggacggcg cataccggta tttagctact acagcgttaa caaagctcgt attcagggcg
    19681 tggaaaccga actgaaaatt ccgttcaacg atgaatggaa actgtcgatc aactacacct
    19741 acaacgatgg tcgtgatgtc agcaacggcg aaaacaaacc gctatccgat ctgccgttcc
    19801 atactgctaa cggtacgctg gactggaaac cgctggcgct ggaagactgg tcattctatg
    19861 tttctggtca ctataccggg cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg
    19921 gttataccat ctggaatacc ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg
    19981 caggcgtgct gaaccttggc gacaaggatc tcagtcgtga cgactacagc tataacgaag
    20041 acggacgtcg ttactttatg gcagtggatt atcgcttctg atgagaagat gcccggcgaa
    20101 ccgggcggac tttcacttca gtaaatactg cgcatggaag cgcaggtgat cctctataaa
    20161 agaggcgatg aagtagtaac tgtgatcata tcccggctga atacgcagcg tcatcggcca
    20221 cgctttctgg cgcgcggctt ctgccaatac cgcaggttgc aactggtcgg caagaaactg
    20281 atcattatcg ccctgatcga taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat
    20341 cagcgcgcaa ctatcccatt ccagccatgc atttttgtcc tcacctaaat aggtgctaaa
    20401 cgctttgatt ccccacggga cgctgcacgg attcacaatg ggcgcaaagg ccgaaacgct
    20461 ggtgtattta cccggatttt tcagcgccat aatcagcgca ccgtgaccac ccattgagtg
    20521 accgctaatg gcgcagcggt cgctgacatt aaattgcgac tgaaccagcg ccggtaattc
    20581 atcgcgcaga taatcataca tccggtaatg cgtcgcccac ggcggttgcg tggcattaag
    20641 ataaaagcct gcgccctggc ctaaatcgta gccatcgtcg ttggcaacct tttcgccgcg
    20701 cgggctggtg tctggcatca ccagtacaat ccccagttcc gccgctaccc gctgggcacc
    20761 cgccttggtg gtgaagttct cgtcattgca ggttaatccg gaaagccagt acagcactgg
    20821 tggcggggtg tgatcacgag ggggtgggag aaagatactg aacgtcatcg ggcagtttaa
    20881 ggtactggag tcgtgtcgcc agcgttgctg ccagccttca aaacagcggt gctcttcgag
    20941 catttccatg cgtggctcct tgttgtgttg tttgcaattt cttcatcata ccgtttattc
    21001 aatgtgctgt gagtaacttt cacttccgta tttgcataac gatgttttaa catctgctga
    21061 tgaaaggcag cggcaattac aataattatc gctgtgaata ctggattatg tgcgccgcct
    21121 cacgcacaat aatcaagctg taaatcagct taataacttt gcccccacgc agggcggagg
    21181 cgtcacacct gcaggagaaa tcataaatgc catcactcag taaagaagcg gccctggttc
    21241 atgaagcgtt agttgcgaga ggactggaaa caccgctgcg cccgcccgtg catgaaatgg
    21301 ataacgaaac gcgcaaaagc cttattgctg gtcatatgac cgaaatcatg cagctgctga
    21361 atctcgacct ggctgatgac agtttgatgg aaacgccgca tcgcatcgct aaaatgtatg
    21421 tcgatgaaat tttctccggt ctggattacg ccaatttccc gaaaatcacc ctcattgaaa
    21481 acaaaatgaa ggtcgatgaa atggtcaccg tgcgcgatat cactctgacc agtacctgtg
    21541 aacaccattt tgttaccatc gatggcaaag cgacggtggc ttatatcccg aaagattcgg
    21601 tgatcggtct gtcaaaaatt aaccgtatcg tgcagttctt tgcccagcgt ccgcaggtgc
    21661 aggaacgtct gacgcagcaa attcttattg cgctacaaac gctgctgggc accaataacg
    21721 tggctgtctc gatcgacgcg gtgcattact gcgtgaaggc gcgtggcatc cgcgatgcaa
    21781 ccagtgccac gacaacgacc tctcttggtg gattgttcaa atccagtcag aatacgcgcc
    21841 acgagtttct gcgcgctgtg cgccatcaca actgattaaa aggcaggaac catggagcgc
    21901 aacgtcacgc tcgattttgt tcgcggcgtc gccattctgg ggatcctgct attaaacatc
    21961 agcgcctttg ggctaccaaa ggcggcttat ctcaatcccg cctggtacga tgctattacg
    22021 ccgcaggatg catggacctg ggcatttctc gatctcatcg gccaggtgaa attcctcacg
    22081 ctttttgcgc tgctgtttgg tgctggcctg caaatgttgc tgccccgtgg cagacgctgg
    22141 atccagtcgc ggttaacgct gttagtcttg ttgggcttta ttcacggttt attgttctgg
    22201 gacggcgata ttctgctggc ttacgggctg gtgggcttaa tctgctggtg gctggtgcgc
    22261 gatgcgccat cggtaaaaag cctgtttaat accggcgtca tgctttatct ggtggggctt
    22321 ggcgttttgc tgttattggg gctgatttcc gacagccaga ccagccgcgc ctggacgccg
    22381 gatgcatcgg ctattttgta tgaaaaatac tggaagcttc acggcggcgt tgaagcgatc
    22441 agtaatcgtg ccgatggtgt tggcaatagt ttactggcac tgggcgcaca gtatggctgg
    22501 caactggcag ggatgatgct cattggtgcg gcgctgatgc gcagcggctg gctgaaaggg
    22561 cagttcagct tacgtcacta tcgtcgtact ggttttgtgc tggtggcgat tggggtgacc
    22621 attaaccttc ctgccatcgc cctgcaatgg cagctggact gggcgtatcg ctggtgtgcc
    22681 ttcttacttc aaatgccgcg ggaactgagt gcgccgtttc aggcgattgg ctatgcgtcg
    22741 ctgttttatg gcttctggcc gcaattgagc cgctttaagc tggtgcttgc gatcgcctgc
    22801 gtcggacgga tggcgctgac caactatcta ttgcaaacgc tgatttgtac cacgcttttt
    22861 taccacctcg gtttgtttat gcattttgac cgcctggagc tgctggcgtt tgttattccg
    22921 gtatggctgg cgaatatcct cttctctgtt atctggctgc gttacttccg ccaggggccg
    22981 gtggaatggc tctggcgtca gttaactttg cgtgctgccg gactggcaat atctaaaaca
    23041 tcaagataac gatctggatc acattcgtta acaaaacggc tgtaaccgtt tccattgctg
    23101 tgactcgatt cacgaagtcc tgtattcagt gctgacaaaa tagccgccag caagcagtca
    23161 tttactgcaa tctcataaca ggtagtgaat atgatcacca ttcgtgatgt agcgcgtcag
    23221 gctggcgtct ctgtggcaac ggtttcccgg gtgctcaata acagcacgct ggtcagtgcc
    23281 gacacgcgtg aagcagtaat gaaagccgtg agtgagctgg attatcggcc aaacgccaat
    23341 gcccaggcgc tggcaactca ggttagcgac accattggcg tggtggtgat ggacgtttct
    23401 gatgcgtttt tcggcgcgct ggtaaaagcg gtggatctgg tcgcccagca gcatcagaaa
    23461 tacgtgctaa tcggcaatag ctatcatgaa gcggaaaaag agcgtcacgc cattgaggtg
    23521 ttaattcgcc agcgttgtaa tgcgttgatt gttcactcaa aagcattgag tgacgatgaa
    23581 ctggcacaat ttatggataa cattcccggt atggtgttaa tcaaccgcgt tgtgccgggg
    23641 tacgcccatc gttgcgtttg cctggataat ctcagcggtg cccgaatggc gacgcgcatg
    23701 ttgctgaata acggtcatca acgtattggt tatctttctt ccagccacgg cattgaagat
    23761 gacgccatgc gtaaagcagg ctggatgagt gcgttgaaag agcaggatat tattccgccg
    23821 gaaagctgga ttggcactgg tacgccggac atgccgggcg gtgaggcggc gatggttgaa
    23881 ctgctggggc gcaatctaca acttaccgct gtatttgctt ataacgacaa tatggccgct
    23941 ggcgcactga cagcattaaa agataatggc attgcgattc cgttacatct ctcaatcatc
    24001 ggtttcgatg atattcccat cgcccgttac accgacccgc aattaacgac cgtgcgttat
    24061 cccattgctt caatggctaa attagccacc gaactggcct tgcagggggc agcaggcaat
    24121 attgatcctc gtgccgccac tgttttatgc cgacgttagt gcgtcgccat tctgtcgcaa
    24181 cgcgccagaa tgcggcggcg atcactaact caacaaatca ggcgatgtaa ccgctttcaa
    24241 tctgtgagtg atttcacagt atcttaacaa tgtgatagct atgattgcac cgttttaatg
    24301 ttgtaacccg tatgtaacag tgaataatca ctttcaccga ggtaatagcg tcataacaac
    24361 aattaaagcc gttttctgga gcgttaccgg gcatggaaga acgaatttta aaaagtgagc
    24421 ttcggcgttc agtaacactt cattaactct actgccccgc cgagcattta tctcaagcac
    24481 taccctgcat aagaaaaacc ggagatacca tgaataagaa ggtgttaacc ctgtctgctg
    24541 tgatggccag catgttattc ggtgccgctg cacacgctgc tgatactcgc attggtgtaa
    24601 caatctataa gtacgacgat aactttatgt ctgtagtgcg caaggctatt gagcaagatg
    24661 cgaaagccgc gccagatgtt cagctgctga tgaatgattc tcagaatgac cagtccaagc
    24721 agaacgatca gatcgacgta ttgctggcga aaggggtgaa ggcactggca atcaacctgg
    24781 ttgacccggc agctgcgggt acggtgattg agaaagcgcg tgggcaaaac gtgccggtgg
    24841 ttttcttcaa caaagaaccg tctcgtaagg cgctggatag ctacgacaaa gcctactacg
    24901 ttggcactga ctccaaagag tccggcatta ttcagggcga tttgattgct aaacactggg
    24961 cggcgaatca gggttgggat ctgaacaaag acggtcagat tcagttcgta ctgctgaaag
    25021 gtgaaccggg ccatccggat gcagaagcac gtaccactta cgtgattaaa gaattgaacg
    25081 ataaaggcat caaaactgaa cagttacagt tagataccgc aatgtgggac accgctcagg
    25141 cgaaagataa gatggacgcc tggctgtctg gcccgaacgc caacaaaatc gaagtggtta
    25201 tcgccaacaa cgatgcgatg gcaatgggcg cggtagaagc actgaaagca cacaacaagt
    25261 ccagcattcc ggtgtttggc gtcgatgcgc tgccagaagc gctggcgctg gtgaaatccg
    25321 gtgcactggc gggcacagta ctgaacgatg ctaacaacca ggcgaaagcg acctttgatc
    25381 tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg caccaactgg aaaatcgaca
    25441 acaaagtggt ccgcgtacct tatgttggcg tagataaaga caacttggct gaattcagca
    25501 agaaataaga ctgattgggc gcagtcgttt actgcgccct tattgtatgg ccaacaaggt
    25561 aaaattatat ggtcagctca acgactccgt cttccgggga atacttgttg gaaatgagcg
    25621 gtatcaacaa gtcctttcct ggtgttaagg cacttgataa cgttaattta aaagtccggc
    25681 cacattctat ccatgcatta atgggggaaa acggtgcagg aaaatcgaca ttattaaaat
    25741 gcctgtttgg tatttatcaa aaagactccg gcaccatttt attccagggt aaagagatcg
    25801 atttccattc cgccaaagag gcactggaaa atggtatttc gatggtacac caggagttaa
    25861 acctggtatt acaacgttcg gtgatggata acatgtggct ggggcgatat cccaccaaag
    25921 gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa agcgattttt gatgaactgg
    25981 atattgatat cgatccgcgt gcgcgcgtcg gcacattatc cgtttcgcaa atgcagatga
    26041 tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt gattatggat gaaccgactt
    26101 cttcgttaac cgaaaaagag gtcaatcatc tgttcactat tattcgtaaa ttaaaagagc
    26161 gcggctgcgg tattgtttat atctcgcata aaatggaaga aatcttccag ttatgtgatg
    26221 aagttaccgt attgcgcgac ggtcagtgga tcgccaccga accgctggca ggactgacga
    26281 tggacaagat catcggcatg atggttgggc gttctcttaa tcagcgtttt cctgacaaag
    26341 aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct aacgtcactg cgccagccgt
    26401 cgattcgcga tgtctcgttt gatctgcata aaggggagat cctcggtatt gccgggctgg
    26461 tgggggcgaa acgtaccgat attgttgaga cgttatttgg tattcgcgag aaatcggctg
    26521 gcaccattac gttgcacggc aaacagatca ataaccataa tgccaacgaa gccataaacc
    26581 acggatttgc actggtaact gaggagcgcc gctcaacggg aatttatgcc tatctggata
    26641 ttggttttaa ctcgttaatt tccaatattc gcaactacaa aaataaagtt ggtttactgg
    26701 ataactcgcg gatgaaaagc gatacccagt gggtgattga ttcgatgcgg gtaaaaacgc
    26761 cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa tcagcaaaag gtgattattg
    26821 gtcgctggct actaacgcaa ccagaaatat taatgctcga tgaaccgacg cgcggtattg
    26881 atgtcggggc gaagtttgaa atttatcagt taattgccga actggcgaag aaaggcaagg
    26941 ggattattat tatctcctct gaaatgcctg agttgttagg gataacagac cgtattctgg
    27001 tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa aacaacaacg caaaacgaaa
    27061 ttctgcgtct tgcgtctttg cacctttaag attaggggct tcccatgagt gcgttaaata
    27121 agaaaagttt tcttacttac ctgaaagagg gcggtattta cgtcgttctt ttagttttgc
    27181 tggcgattat tattttccag gacccaacat ttttaagtct gttgaactta agtaatattc
    27241 tcacccagtc atcggtgcgt attattatcg cgctcggtgt ggcagggtta attgtcaccc
    27301 aggggaccga tctttctgct ggtcgtcagg tagggctggc ggcagtggtg gctgcgacat
    27361 tattgcagtc catggataac gccaacaaag tgttcccgga aatggcgacg atgccgattg
    27421 cgctggttat tctgattgtc tgtgccattg gtgcggtgat cggtttgatc aacggtctga
    27481 ttatcgctta tctcaacgtg acgccgttca ttaccacgct cggcacgatg atcatcgtct
    27541 atggcatcaa ctcgctctat tacgactttg tcggggcgtc gccaatttct ggttttgaca
    27601 gtggcttctc tacctttgct cagggctttg tcgcgctggg gagtttccgt ctctcttaca
    27661 tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt gttgtggaac aaaacccgct
    27721 tcggtaagaa catttttgcc attggcggta acccggaagc ggcaaaagta tctggtgtca
    27781 acgtcggcct gaacctgctg atgatctacg cgttgtctgg cgtgttctat gcctttggcg
    27841 ggatgttaga agccggacgt atcggctctg ccaccaacaa cctcggcttt atgtatgagc
    27901 tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt cagcggcggt gtggggacgg
    27961 tgattggcgt ggtgaccggg gtaattattt ttaccgtcat caactatggc ctgacgtata
    28021 tcggcgtaaa cccatactgg cagtacatta tcaaaggggc gattattatc ttcgccgtag
    28081 cgctggattc actgaaatac gcgcgtaaga agtgatgtga ttgcggatat tgccggatgc
    28141 gctgcatccg gcaatcggtg ccaaatgccg gatgcgtctc tggttttaca acgttaccgg
    28201 gtgttctttc tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca
    28261 gacgtgacca caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt
    28321 tttctcgctc cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc
    28381 acagccaaca catttgtcaa gattgatacg gggatagaca atataactgc ggtctaaatc
    28441 ttccgccggg acaatattgt tattcgccag acctaccatc tcctgcaacg aatcaaatcc
    28501 ctgatcggcg agataatgcg ataacccgct cgccatatct tctacgatgc gatagccgta
    28561 ctgcataatg ccggtggtga cctgtaacgt tgctgcgccg agcaataaaa actccgccgc
    28621 atcttcccag gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg
    28681 ggtgcgcatt tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc
    28741 ggaaatactc gatttaccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt
    28801 ggtgatggat ttaacggtgt taattgccgc aatgccatct gcgccgccgc gcttcgccgc
    28861 cagcgccact tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt
    28921 ggaaccccgt ttcactgccc gacaatattt ttttaccagc tccgggcttt gcccgacatc
    28981 gctacccatc gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc
    29041 gccagcttct tgcaccaggc gcgccagctc ctcccattgc tgctcatttt cccccatgat
    29101 cgaagcgatc aatactttgt ccgggtaatc ttccttcagc cgacgcaggg cggtcagatt
    29161 ctcttccaac ggatgttcag caatctgctc catatttttg aagccgataa aaccggtatc
    29221 ttctttcacc agatgatcaa aacgcggcga gacttcgttg gcgataaaaa agccgatcgt
    29281 tttaaacacc acaccgcccc aacctgtgtc gtaggctttg gcacacatct catagcagtt
    29341 gcctaccggc gaagaagaga ggcagaacgg gttgggaaag ttcacgccgc aaaaagtaat
    29401 cgaaagatct ttcgttaaca tgagcaagct ccctctaata tagtgatgaa tcgcctcggc
    29461 ggcttctttc ccggttttca cggcatagac cacggttttg tcaccctcaa caatatcgcc
    29521 agcagcaaag acttgcggat cgcgggtctg ataattttgt gttttgatgg tgttacgctg
    29581 ctgctctaac tccgcaaagg catccagtct ggcatgctga ccgacggcga gaataatttt
    29641 atctgccgcc atcgtcagtt cgcctgataa ccgtacatgc ttaaacgtga ctttattgcc
    29701 ttccacggct actggcgtga atccatcgat gatcgaaacg cccagctccc gggcgctggc
    29761 aaactctttt tcgcttgccg gaaactcatc taactcttca cgtgctacgc aagttaccgc
    29821 ctgacagccg agaactttca gcgtgctggc tacgtccatc gcgacatcac cgccgccgat
    29881 aattaatgcg ctttgtggaa tgctgatatc gccttgcggc tgtcgtacac gttgcaggaa
    29941 gtcgacggca atctcaacgg tcgctatgct caaacagcgg tagcccggaa ccgcttgata
    30001 accccacggt gaccagtacc gcgcggtttt ccgctttcag ttgctccagg gtgagtgttt
    30061 taccgatttc gttattgcac ttaatggtca cgcccatttt ttcaatacgg gcgatctctg
    30121 catccagcac tgattgcggt aaccggaatt gcggaatacc gttacgcaac cagccaccgg
    30181 ggtgcgcttc tttctcataa atcgtgacgt cataaccctg gtttgtcagt gtcacactgg
    30241 cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt gccgagcgtt ttagtaccgg
    30301 gctgataaat ttccatcccg gtttgttgtt caaaatcagt aacaaaacgc tgtaagcggc
    30361 cgatatcaat gggagcatca acaccggcac gggtacaacc gctttggcat aatttctccg
    30421 tcgggcacac tctggcacaa acggcaccga gggcattatt ttcgcgaatt gtctcggcag
    30481 cgcctttaaa attacgaaag tagattgagc gaataaattt ccccggatcg gtctgtgccg
    30541 ggcaagcctg actacaggga gcgtcgtgac ataataaaca gcgagaggct tctttaatcg
    30601 ccagtaacgg cgtaaaagcc ggagtgagtt catccagata attttgttgc ggcatgtgat
    30661 cttccttaat gctatgagta attatggcga acgctaaata aatattgcgt gctgatatta
    30721 aggctatgcg cgtggggaaa tttaaactgt gaaaggattc acaaatagct gcggttcaga
    30781 atgttatatc cgcagcttat tgagtgaagt aataattatt ctcaataatt aacagaaaag
    30841 agaaaatatt aacgatagaa aataagataa acaatcatgc cgatagataa aattccgaag
    30901 ccaatatatg ccaccatata agcgaagaag agtccgggat tttttgctcg ccagacatac
    30961 accggttccg gtgcattttt aacccgttta tcaatcgcgc ctgcgcgcca gcctttccat
    31021 gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg caacgacaaa aaactgctgt
    31081 acatccataa ctactttcct tgtttctttt gtaattcaag taactgttcg ggtgtaacgg
    31141 cgggataccc ctgcgcatct tccggcacct ggtgcatagc cggaataggg accagcggcc
    31201 ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc cagcgcaccg aaacgggcgg
    31261 caaattcgcg aatacgtact gacagcatat ctttcggtga cggtacggca taacactgag
    31321 cctgaatccc catatgcagc gcaataaata atgctcgctc acagtggaaa cgttgggtga
    31381 taataatgaa atcattagta tcgaaaactt tgcgtgtacg cacgatggaa tccagcgtac
    31441 gaaagcctgc gtaatcgaga acaatatctg atgggtcgac accagcagcg attaaatctt
    31501 tgcgcatggt catcggctcg ttataacttt gcaatgcgtt atcgccgctc agtaatagat
    31561 aatttacctt accgctgtta taggcattaa tcgctccttg aatgcggtag cgataatact
    31621 gattaataac gccggtacga taatattttg ctgtgccgag caccacgccg acctggcggt
    31681 aggggagatc ctgcaattcg tcgtagatat aaggcgcggt tttccagctc atccagcgat
    31741 cgaggccgag cacagtcaac agcagcaagc cgatcaggac taacaggctg aggaacacgc
    31801 gctttaacat gcaggagact cgagctaagg aaaatttcca tcaggctact ttacccgctg
    31861 gttttgcgca agaaacggca aactgtttgc cgggaatttc gacaccagga gagggctggc
    31921 aagccggaca aggcgttcac gccgcatccg gcaccaggct taagcgagaa gcacgcggtc
    31981 gatactgtga cagccgagag ctttcagcgt tgcggaggtg gcatcccact gaatcaacgg
    32041 cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc gggtaatcat aacccttgag
    32101 attcaacaga attaacgccc cctgcaacgg cggcagagtc gggttgaatg cggcgttttc
    32161 agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc agcgcgacac cgcttggtga
    32221 cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc gccgcctgag aaagcgcatc
    32281 accagtcagc gcatagccgt gatcctgctc gtccatcagc agcgttttaa tctccagatc
    32341 tttcggccca aaggcatccg gcagatagtc acgcagcgcg tgtgcctcgc ggcccggcaa
    32401 atgaatacgc agatccagac cgctgttcag ttcattcata aactgacggc agtgaccaca
    32461 aggcgtatag ttaacggtga tggctgcaag cgctttttca ccactcaacc aggcgtggct
    32521 gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt gcaccaataa attccatatt
    32581 ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt gcgccaacat taaaattcga
    32641 caatggcgta cgcgcacagg ccgccgccag cggaagtagt gcgaatgcca gcgcgtcttc
    32701 gtccagcccc gttgcgctct tcagcgatga gacttgctcc ccggtcaaca aagcggggaa
    32761 gtacttgtct gccagaatag gttccagtgc agattgcaag ttatccgcaa gttgggcaaa
    32821 agcggtttga aaacgtggat gcatggcgtt gcctcataat catgtaatgg gataacagtt
    32881 tacgaacccg ttatggcttt atatgtgatc tgaatctcat taattatgca acacgtgaaa
    32941 cttacatcaa aaatgcgcga cgtatcgcaa attttagccc attactgcca gaataatcgg
    33001 gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat aacaccagcg ctagcgaact
    33061 aaatgcacct tcctgataat ccagctcggc gcagcgcgcc gtaccgaggg cgtgcgaggc
    33121 agtccccatt gccagaccgc gcgcagcttt ggtacgaata cgcatcgcat taagcaatgt
    33181 atggccaaat accgcgccga ggatgccgac gaaaatcacg caaactgcgc tgattgccgg
    33241 aataccgcca atactgccgc caaccgccat tgcaataggc gtggtgactg atttcggcag
    33301 gattgacgcg gcgatttccg gtgaagcgcc catcaacaat gccacggaag tccccgtcac
    33361 catcgcaacc acgctgccga taaaacagat ggtgatgatc gatttccagc gcgcgcggat
    33421 ctggtgtagc tgctcatata aaggataggc cagcgcgacg accgccggtt gcagcagatc
    33481 gtttagcact tcgctacctt taaagtaact gtcgtaagag atgccggtca gcattaaaaa
    33541 aggaatgatc accaccattg ctaccagcaa cgggttaagc aggggaaact tataccgtgc
    33601 cgccagtttg cgggcggcaa aaaagacaat caacgtcagc ggtaatgacc accagatatt
    33661 cgccatcatt cttctgatcc tttctgacct acgactttac gttcaccgtg taccagttgt
    33721 gaactccagc tcaccaccag aaaaaccacc agcgtactga cagcacagga aaccactacc
    33781 gggccaaact gcgcgcggag caaatcaaaa tattgcatga cgcctacgcc aatcggcaca
    33841 aacaatagcg ccatatagcg aatcagtacg tagcaccccg gattgaccca tttcgccgga
    33901 agaatttgca aggccagcag gacaaacagg atcagcatcc cgatgatgct gcccggaatg
    33961 gttaccggta gcagggaagc aatgaaaatg cctgcataca ggcaggcata aatcaggacg
    34021 aaagcgcgta aatattgcca gataatgttc agtgtcttac tcataatcaa caccccagat
    34081 gagatggatt catcatacaa ttaattcgta aaacgtgcta ccgatcacat taagaaatca
    34141 gcgcttattt tttcgctacc cggtattacg caatgagcag cgaactcagg catacatcaa
    34201 gcatagagag ggattgcagt ttgcagcaat ccctctttgc agacctttaa tccgctgacc
    34261 ataggcgcta acatagcgcc tcattttttt gcgggtgatg atatacgtgt gttactggca
    34321 ccgatggagg gagtgcttga ctctctggtg cgtgaattgc tgaccgaagt taacgactac
    34381 gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac tgctgccggt aaaagtcttt
    34441 catcgcattt gccctgagct acaaaacgcc agccggacac catctggtac gctggtgcgc
    34501 gtgcagttgt taggtcagtt cccacaatgg ctggcagaga acgccgcccg tgcggtggag
    34561 ttaggttcct ggggcgtgga tctcaattgc ggctgcccgt cgaaaacggt taacggtagc
    34621 ggcggcgggg cgacgttact caaagatcct gaactcatct accagggggc aaaagcgatg
    34681 cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag tgcgtctggg ctgggacagc
    34741 ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg ctggcgctac ggagctggtg
    34801 gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc atattgactg gcaggcgatt
    34861 ggcgagattc gccagcggct gaatattccg gtgattgcca acggtgaaat ctgggactgg
    34921 cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg cagtgatgat tggtcgcggg
    34981 gcgctcaata ttcccaacct gagccgggtg gtaaaatata acgaaccgcg aatgccgtgg
    35041 ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg aaaagcaggg cgataccggg
    35101 ttatatcacg ttgcgcggat taaacagtgg ttgagttatt tgcgtaaaga atacgatgaa
    35161 gcaacggaat tatttcagca tgttcg
//
LOCUS       DAJTXY010000027        33958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTXY010000027 DAJTXY010000000
VERSION     DAJTXY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.027"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(83..2176)
                     /locus_tag="OO566_001446"
     CDS             complement(83..2176)
                     /locus_tag="OO566_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6604781.1"
                     /translation="MQSYVTVNDLLGVPGMPATAKGIRQALQRFSRALGDVSRRRAGT
                     KAIEYHIDCLPEVTQKALRERYVEQLVATENNVSEVKTVTRTARNPDAVQAVEAYRGS
                     PQLMEERLNALTENQRRVSEARAALAVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAINANAKRGTNRTISRDPLYQWVLKYNRSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQANGRPMSEAYEDFVTEWQRRYADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEIV
                     GILPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRETLRVLNRD
                     LRSAFNALQQDKPLNARQKSAMRELPSWSELIEAIREGVEWYNNRPHSELPMKPNGKH
                     YSPAEFRKKRLAEENTEIEWLSDLELRDMFCPMVERPVRRCEIKWLGNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFPVSADYYKQQQRIKGMHK
                     RAEEKIRDAVDEGIQVIEQKTTEPWLDNVYRPVGNVMTVQQQEYEEEHDEESERNFRR
                     GLQKLIAMQDDDPLA"
     gene            complement(2178..2429)
                     /locus_tag="OO566_001447"
     CDS             complement(2178..2429)
                     /locus_tag="OO566_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6604782.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            2582..3145
                     /locus_tag="OO566_001448"
     CDS             2582..3145
                     /locus_tag="OO566_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604783.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(3421..3843)
                     /locus_tag="OO566_001449"
                     /pseudo
     CDS             complement(3421..3843)
                     /locus_tag="OO566_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(3843..4436)
                     /locus_tag="OO566_001450"
     CDS             complement(3843..4436)
                     /locus_tag="OO566_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6604784.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            4583..4990
                     /locus_tag="OO566_001451"
     CDS             4583..4990
                     /locus_tag="OO566_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6604785.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(5110..6102)
                     /gene="rpoS"
                     /locus_tag="OO566_001452"
     CDS             complement(5110..6102)
                     /gene="rpoS"
                     /locus_tag="OO566_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6604786.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(6165..7304)
                     /gene="nlpD"
                     /locus_tag="OO566_001453"
     CDS             complement(6165..7304)
                     /gene="nlpD"
                     /locus_tag="OO566_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6604787.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(7444..8070)
                     /gene="pcm"
                     /locus_tag="OO566_001454"
     CDS             complement(7444..8070)
                     /gene="pcm"
                     /locus_tag="OO566_001454"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6604788.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8064..8825)
                     /gene="surE"
                     /locus_tag="OO566_001455"
     CDS             complement(8064..8825)
                     /gene="surE"
                     /locus_tag="OO566_001455"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6604789.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(8806..9855)
                     /gene="truD"
                     /locus_tag="OO566_001456"
     CDS             complement(8806..9855)
                     /gene="truD"
                     /locus_tag="OO566_001456"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6604790.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICAKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(9852..10331)
                     /gene="ispF"
                     /locus_tag="OO566_001457"
     CDS             complement(9852..10331)
                     /gene="ispF"
                     /locus_tag="OO566_001457"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6604791.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(10331..11041)
                     /gene="ispD"
                     /locus_tag="OO566_001458"
     CDS             complement(10331..11041)
                     /gene="ispD"
                     /locus_tag="OO566_001458"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6604792.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11060..11371)
                     /gene="ftsB"
                     /locus_tag="OO566_001459"
     CDS             complement(11060..11371)
                     /gene="ftsB"
                     /locus_tag="OO566_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6604793.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(11565..11888)
                     /locus_tag="OO566_001460"
     CDS             complement(11565..11888)
                     /locus_tag="OO566_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6604794.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(11938..12543)
                     /gene="cysC"
                     /locus_tag="OO566_001461"
     CDS             complement(11938..12543)
                     /gene="cysC"
                     /locus_tag="OO566_001461"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6604795.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(12543..13970)
                     /gene="cysN"
                     /locus_tag="OO566_001462"
     CDS             complement(12543..13970)
                     /gene="cysN"
                     /locus_tag="OO566_001462"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6604796.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13972..14880)
                     /gene="cysD"
                     /locus_tag="OO566_001463"
     CDS             complement(13972..14880)
                     /gene="cysD"
                     /locus_tag="OO566_001463"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6604797.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15132..16169
                     /gene="iap"
                     /locus_tag="OO566_001464"
     CDS             15132..16169
                     /gene="iap"
                     /locus_tag="OO566_001464"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6604798.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(16315..17406)
                     /locus_tag="OO566_001465"
                     /pseudo
     CDS             complement(16315..17406)
                     /locus_tag="OO566_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(17600..17752)
                     /locus_tag="OO566_001466"
     CDS             complement(17600..17752)
                     /locus_tag="OO566_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6604799.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(18016..18750)
                     /gene="cysH"
                     /locus_tag="OO566_001467"
     CDS             complement(18016..18750)
                     /gene="cysH"
                     /locus_tag="OO566_001467"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6604800.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMVEEETRFFGLKRECGLHEG"
     gene            complement(18824..20536)
                     /gene="cysI"
                     /locus_tag="OO566_001468"
     CDS             complement(18824..20536)
                     /gene="cysI"
                     /locus_tag="OO566_001468"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6604801.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(20536..22335)
                     /gene="cysJ"
                     /locus_tag="OO566_001469"
     CDS             complement(20536..22335)
                     /gene="cysJ"
                     /locus_tag="OO566_001469"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6604802.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            22654..23016
                     /gene="queD"
                     /locus_tag="OO566_001470"
     CDS             22654..23016
                     /gene="queD"
                     /locus_tag="OO566_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6604803.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            23094..24365
                     /locus_tag="OO566_001471"
     CDS             23094..24365
                     /locus_tag="OO566_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6604804.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            24356..24616
                     /locus_tag="OO566_001472"
     CDS             24356..24616
                     /locus_tag="OO566_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6604805.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            24633..25208
                     /locus_tag="OO566_001473"
     CDS             24633..25208
                     /locus_tag="OO566_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR6604806.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(25356..26216)
                     /locus_tag="OO566_001474"
     CDS             complement(25356..26216)
                     /locus_tag="OO566_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6604807.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIDVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(26213..26992)
                     /locus_tag="OO566_001475"
     CDS             complement(26213..26992)
                     /locus_tag="OO566_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6604808.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTLVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26970..28379)
                     /locus_tag="OO566_001476"
     CDS             complement(26970..28379)
                     /locus_tag="OO566_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6604809.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMSGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SIFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(28401..29855)
                     /locus_tag="OO566_001477"
                     /pseudo
     CDS             complement(28401..29855)
                     /locus_tag="OO566_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(29925..30711)
                     /locus_tag="OO566_001478"
                     /pseudo
     CDS             complement(29925..30711)
                     /locus_tag="OO566_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            31030..32307
                     /locus_tag="OO566_001479"
     CDS             31030..32307
                     /locus_tag="OO566_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6604810.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            32334..33804
                     /locus_tag="OO566_001480"
                     /pseudo
     CDS             32334..33804
                     /locus_tag="OO566_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         8158 a         9001 c         8381 g         8418 t
ORIGIN      
        1 tcggtcatat tgtttcctcg gttatttcaa atcagcttta tttacctgtt taacagtccg
       61 ttgctgcgga ctgttttttt attcaggcca gtgggtcatc gtcctgcatg gcaatgagtt
      121 tctgtaatcc ccgcctgaaa ttgcgttcgg attcttcgtc gtgctcctct tcatattcct
      181 gttgctgaac ggtcatcaca ttgccaacgg gtcgataaac attatccagc cagggttctg
      241 tcgttttttg ttcaataacc tgaatacctt catccacggc atcacggatt ttttcttctg
      301 cgcgtttgtg cattccttta atacgctgct gttgtttgta atagtcggcg ctgaccggga
      361 aggcggcgcg tttattgccg ttccatatcg cctcacaaat aaaactgcca tccttacggc
      421 gcactgtaat tcgctctgca tcatgaatgt catagctgat aagcactttg cgaccatgtt
      481 catcacgcag ctccggcgcg taataaatat tcccaagcca tttgatttca cagcgtctta
      541 ccggacgctc caccatcggg cagaacatgt cgcgcagttc gagatcactc agccattcaa
      601 tttcggtgtt ttcttccgcc aggcgttttt ttctgaactc agccgggctg taatgcttcc
      661 cgtttggttt catcggcagt tcagaatgcg ggcggttgtt gtaccattcc actccttcac
      721 gaatagcctc aatcagttct gaccaggacg gcagctcgcg cattgcagat ttctggcggg
      781 cattcagcgg tttgtcctgt tgcagggcat taaatgctga acgcagatcg cgattgagta
      841 cacgcagtgt ttcccggtct gcccccttgc caaaataggt gcgataccgt ctggctatac
      901 gcatcggcag cgtccggttc agacgttcaa tgatgccgcg tccctgcgga tttccggcaa
      961 taccggtggg gtggttaata cccagacggg gcagaatccc gacgatctct ttatccagca
     1021 tatctgccgt ttcaccggaa ccattatccg agtaatacag gaagggtttt ccgtgattgc
     1081 gaattccgtg ctgtatcgcc ccggcaacag caaacacgtt ctccgcaaga tcaagactcc
     1141 agccaacaac aaaccgggtt ccaccgtcga taacaaacgt gacttccggt gaaaatggac
     1201 gcccgtggat cggatgcgca catttcagct tcataccgtg gccgtcacca atccagacat
     1261 aattcaccgg catagctgtc cagtcgcggc gggtgaatcc ctcaagctgg cggtattcac
     1321 tgccggtaac gcgtcctttt tgtttcacca cttccggcaa ttttttcatg gcataccgga
     1381 caacatcata ggaaggcatc acctccagca tgtacggttc gtccgcatag cgacgttgcc
     1441 attccgtgac aaaatcctcg taggcttccg acataggtct gccgttagcc tgacgatact
     1501 gcgccagaaa ttcaggcagc cagctaattt cttccggttt tatttcgtcg cgttttcctg
     1561 gtgccagtaa caacagtcgt tctgcggcgt tctgcgaccg gttgtatttc agtacccact
     1621 ggtaaagcgg atcacggctg atggtgcgat tagtgccacg ttttgcatta gcgttaatcg
     1681 cagcctgttg cagtcgttcc ggcaactcac ctttacgggc tttttcaacc agaaagttaa
     1741 tggctttcag tcgcccgagg ttaccggcgc tttccagctt cagcacttcc actgccagcg
     1801 ccgctcttgc ttcagacacc cggcgctgat tttcagtcag ggcattcagg cgttcttcca
     1861 ttagttgcgg agaaccccga tatgcttcaa ctgcctgaac agcatcaggg ttacgcgccg
     1921 tcctggtaac ggtcttcact tcggagacgt tgttctctgt agccaccagt tgttccacat
     1981 agcgttcacg cagtgccttt tgggttacct caggcaggca atcgatatgg tactcaatgg
     2041 cttttgtacc tgctcgacgg cgactaacgt caccaagagc acgagaaaaa cgctgtaacg
     2101 cctgtcggat gcctttcgcg gtggctggca ttccaggaac accgagaagg tcatttacgg
     2161 taacgtacga ctgcatatca cgacgccttc ctgataaatc catcgttacg ataccggctg
     2221 ggccagatga tttctggcgg tacacctata gcgtcagaaa ttacgcgttc gtattttttg
     2281 tttggtgtgc gaactaccgc tttcagtgaa tcctgagcat aaccagcatg gagtgaaagc
     2341 tcccggaagg aagaccaccc gttatctttg agcgccagct gaatcttcag cgaaggccaa
     2401 tcgcggtcac tatcttctct ttgtctcatc tttcctttaa cctacaaaat aacccgcgcg
     2461 gatatccgta tggataaaga ttaagatacc aaagattccc tataaagggt tctttggtgc
     2521 ttttatggtt tattttttaa tttcattatt tatcaatgaa ttagatagaa aggaaccaag
     2581 aatgagaaaa gcaccccaag gttcttttcc tgccgatgga aaagaaccga ttatcgaaag
     2641 aatctttagg ttagtagaac gatacccttc aagaaatgcg gctgctcggg catgggggat
     2701 caatgaaggc actttaaaaa attattacaa caggcgggat tacgcaccga taccacgccc
     2761 gcatcagtta aaaaagatag cagaaagcga aaatgttacc ttggaatggc tcatgacggg
     2821 agagggtgat gttgaactaa aagattccaa agaacccaaa aaagagaatg cggataatgt
     2881 tgatgctcaa attctaatgt tcttgtcatt tcttaaacca caagaaaaac aacaagtagc
     2941 tgatgttctt gggagaaaag gagcggagca attactcatt cttctggatg agcacatcca
     3001 agagttgcac gctctagttg gagttcgtag agccatagcg ttaagccttg gtaatttgcc
     3061 agacgaaaag gttagagaga tttacgcgtt gtccgaagct aagggcgatc acttaaatct
     3121 acctcagaaa gcagccagcg cataacccag tgcaagtgcc tagctgtgct aaaattacat
     3181 tggttttggc gcttgcaccc atcagcgcta taataaacac ctgaatcgtt tttgaacggt
     3241 ctttaaaggc tattgtaaga acctgtgagt tatcttaaat gttttattga tatcgaaccg
     3301 tttaacattt ttggactcgc tttatcttga aaaccttgag ttcctaactt gttgattcca
     3361 atctattccc gtctttttta atctcttccc ggttatcttg aataaattac ctagtcatac
     3421 gagatagaaa ccgccatcgc catcattcag gcgaaacagc ggcagaatat cgaacaggtt
     3481 gatctcttcg ccatcgacgg tgttctgcgc ccaggctgga ttggcgcggc gctccggggc
     3541 aatcgggaag ttatcccagc ggcggataaa ctcatcaatc tgctttttga ccggggtgtt
     3601 tggtggcagg ccaagggaaa tcgcgtggtt ctgccaggaa ccgatggtat tcatcgccac
     3661 gcgggcatcg gtaaagccgc gaatattatc aaaccacagc gcgggcgcgc cgtcgccgat
     3721 acgcccggtg gcgttagctg ctgctgccag atccggctca gcgttcactt cttcgctgat
     3781 tttcagtaac tggccgtggt catcaagcgc ctgtaaaaag ctgcgtaaat catcaaatgc
     3841 cattattcat tctcctgaga aaaattccgg gcctgcggca atccttgcca gcgcctggcg
     3901 tggggatgtt cgaggccaaa ttgatccagc acgcgggcta ccacatggtg gacaatgtca
     3961 tctaccgttt cgggatggtt ataaaaggca ggcatcggcg gcaccatcgc cacgcccatg
     4021 cgtgaaagtg cgagcatatt ttcgagatgg atggtgctaa gcggcatttc acgcggcacc
     4081 agcaccagtt tgcggccttc tttgagcacg acgtccgccg cgcgccctac caggccatcg
     4141 gcgtaaccag cgcggatacc ggcgagcgtt ttcatactgc acggaataac gatcatgccg
     4201 tcagtacgaa aagaaccgga tgagatgatc gccgcctgat ccgccgggtt atggctgaag
     4261 tctgcgaggg cagcaacatc gcgagcgctg taaggcgttt ctagttcaat gatggttttc
     4321 gcccacttcg acatcaccag atgagtctcg acattcggca tctcacgcag cgcttgcagt
     4381 aatgccacac caagaggcgc accggtagcc cctgtcatcc cgacgatcag tttcattgct
     4441 tattctctta attgttcgta tacgaacatt attattaaca tactccttca tttccacagc
     4501 ggcaaggatt gaaaagtaac aacatcttct tcgtcactaa aaaaccgcta tgatagcgac
     4561 agagaattag cccggagttt acatggcgtt acgaaataaa gcgtttcatc agttacgaca
     4621 gctttttcag cagcacacgg ctcgctggca gcacgagtta cctgacctga ccaaaccaca
     4681 gtacgcggtg atgcgcgcca ttgctgataa acctggtatt gaacaggtgg cactgataga
     4741 agcagcagtc agcaccaaag caacgctagc agaaatgttg gcaagaatgg aaaatcgtgg
     4801 cttagtcaga cgtgaacatg acgtcgctga caagcggcga cgctttgtct ggctgactgc
     4861 cgaaggagaa aagctacttg ctgcggcgat acctattggt gacagcgtgg atgaggaatt
     4921 tttggggcgt ttgagtggag aagagcagga gctgtttatg cagttggtgc gcaagatgat
     4981 gagcacatag aatgctgatt gccgggtagg acgctgacgt gtcttatcca ggcgacaaaa
     5041 acacaagctt cccaaacaaa agaaaaaggc cagcctcgct tgagactggc ctttctgaca
     5101 gatgcttact tactcgcgga acagcgcttc gatattcagc ccctgcgttt gcaggatttc
     5161 gcgcaaacgg cgcaggcctt caacctgaat ctggcgaaca cgttcacggg tgaggccaat
     5221 ttcacgacct acatcttcca gtgttgccgc ttcgtacccc agcaaaccga atcgacgtgc
     5281 cagtacttca cgctgtttgg cgttcagctc gaacagccat ttgacgatgc tctgcttcat
     5341 atcgtcatct tgcgtggtat cttccggacc gttctctttt tcatcggcca ggatgtccag
     5401 caacgctttt tcggaatcac cacccagcgg ggtgtctacc gaggtaatgc gctcgttaag
     5461 acgaagcata cggctgacgt catcaactgg cttatccagt tgctctgcga tctcttccgc
     5521 acttggttca tggtccagct tatgggacaa ctcacgtgcg gttcgcaggt aaacgttcag
     5581 ctcctttacg atgtgaatcg gcaaacgaat agtacgggtt tggttcataa tcgcccgttc
     5641 aatcgtctgg cgaatccacc aggttgcgta tgttgagaag cggaaaccac gttccgggtc
     5701 aaacttctct accgcgcgga tcagccccaa gttgccctct tcgataaggt ccagcaacgc
     5761 cagaccacga ttgccataac ggcgggcaat ttttaccacc agacgcaagt tactctcgat
     5821 catccggcgg cgagaggcga catctccacg cagtgcgcga cgcgcaaaat aaacttcttc
     5881 ttcggccgtt aacagtggtg aataaccaat ctcaccaagg taaagctgag tcgcgtccaa
     5941 cacacgctgt gtggctccct gcgataacag ttcctcttcg gccaaatcgt tatcactggg
     6001 ttcctcttct actaaggcct tttcgtcaaa aacctcaact ccgttctcat caaattccgc
     6061 atcttcattt aaatcatgaa ctttcagcgt attctgactc ataaggtggc tcctacccgt
     6121 gatcccttga cggaacattc aagcaaaagc ctggttccgc cgatttatcg ctgcggcaaa
     6181 taacgcagcg ggtttacgga tttccccttg taacgaattt caaaatgcaa gcgtgttgaa
     6241 ctggttccgg tgctacccat ggttgctatt ttttgccccg ccttcacttc ttgttgttcc
     6301 cggaccagca ttgtgtcgtt atgggcgtag gcactcaggt aatcatcatt atgtttgatg
     6361 ataatcagat taccgtagcc gcgcagcgcg ttaccagcat aaacaacgcg gccatctgcg
     6421 gtcgcgataa ttgcctgtcc tttgctgcct gcgatatcaa tccccttgtt gcccccctca
     6481 gaagcgccaa aggtttcgat cactttgccc tcagtcggcc agcgccaggt ggagataggc
     6541 gtactggttg atgtactgct gacagtcggc tcggttgtgc ttgctgttgg taccgttaca
     6601 ggcgctgtga ccgtggtcgc agttggcttg ttgttcggca acattttgtt agcactctgt
     6661 tcacccgaag actcagaata cgtaattgtc ggttgcgacg caacagcaac ggtggaattt
     6721 tgtgcaggct tgatcacaac tccttgctct gctgcgtcgg cctgggtaat agcatttccg
     6781 ccagtgattg gcgtaccgga agcattaccc acctgcaagg tctgaccaac gttcagcgcg
     6841 tatggtgcct gaatattgtt gcgctgagca aggtcacgga aatcgttgcc agtaatccag
     6901 gcgatataga aaagtgtgtc gccttttttc acggtatagg tactgccgct ataactgcct
     6961 ttcggaatgt tcccatactg acggttatag acgatgcgtc cgttttccat ctgtaccggc
     7021 tgctgagcta ctggctgcac tggctggatt tgcggttgtt gagtagcctg aatttgtggc
     7081 tgctgtaccg gctgaatttg cggttgctgc gctgtagacg tcgtccctat tttcggcggc
     7141 ggcgtaatca acataccaga attagtattt gcaggcgcat tgccattaac ggagctgacc
     7201 ggtgccggtg gatttgaagt gtcagaacag cctgccagcc atagcgaaac cagtgacaaa
     7261 gccgcaatgc ggcgaacggt gaattttggg cttcccgcgc tcatttatcc cccaggaaaa
     7321 attggttaat aaccagtgac ataattaccg tgcaaggcac cctactgaac actggaaaag
     7381 atgttcacga tacgctgacc tacggcaaaa taaccaggaa aaatccaggt atttcctcac
     7441 gttttaagcc agctcaccct tcactaaagg gacaaagcgc acggcctcca cggtatcgat
     7501 aataaattcg cctccccgac gacgcacccg tttcaaatac tggtgctcct cccctacggg
     7561 taagacgaga atcccgcctt cgtccagctg cgtcattagc gcagttggaa tttccggcgg
     7621 tgccgccgta acaatgatag cgtcaaacgg cgcacgtgcc tgccaacctt gccatccatc
     7681 accatgacgg gttgaaacat tatgtaaatc aagatttttc aggcggcgac gtgcctgcca
     7741 ctgcaagcct ttaatccgtt caaccgagca aacatgctgg acaagatgcg ccaggattgc
     7801 cgtttgatat cccgaaccag tgccaatttc cagcacccgc gactgcggcg tcagctcgag
     7861 taattcggtc attcgcgcca ccatatacgg ctgcgaaatt gtctgcccct gacctatcgg
     7921 caaggcgata ttgtcccagg ctttttgttc aaacgcttca tcaacgaatt tttcacgcgg
     7981 cacggcggca agtgcattca gcacctgctc atcctgaata ccttgcgcac gtaattgatc
     8041 cagaagtgct tgtacgcgtc tgcttaccat tgcgtgccaa ctcccacgct gtttaaccag
     8101 tctgaaacca catcttgcgc gctatgcgca gttaaatcca catgcagcgg cgtgatggag
     8161 acatagccct catctaccgc agcaaaatcg gtccccggac cggcatcaca tttaccgccc
     8221 ggcgggccaa tccagtacag cgtattgccg cgcggatctt gctgcgggat cacctgatct
     8281 gccggatgtc gtgtaccgca gcgcgtcacg cgaatacctt tgatttgatc caagggtaaa
     8341 tccggaacgt taatattaag aatacgtccg gtgcgcagcg gctctttaca cagtgcgcgc
     8401 aaaattgaac aggttaccgc cgcggcagtg tcgtaatgtt tatgcccgtc aagcgagacg
     8461 gccagcgccg gaaaacctaa atgacggcct tccatcgcgg cggctaccgt accggaataa
     8521 ataacatcat cccccagatt cggcccggcg ttaattccgg acacaacaat gtccgggcgc
     8581 ggacgcatca gagcattcac gccaagatag acacaatcgg tcggggttcc catttgcaca
     8641 gcaatatcac cattttcaaa ggtaaacgtg cgcagggagg attccagtgt cagagaattt
     8701 gaagcgctgc tgcggttacg atcgggggcg accacctgaa cgtcagcaaa ctcacgcaag
     8761 gctttcgcca gcgtttgtat accgggtgca tgtaccccgt catcattact cagcaatatg
     8821 cgcataatca cctgttgtgt tgataagttc cctgacaacg ctggttgcaa aactacccgc
     8881 cggaagccag aaacggatct ctacggtgac gtcatcccac cagttccagc ttaattgttg
     8941 cggatacagc agcatcgctc tgcgcgcggc ttcaactttt tcgcgcacca gtaaagcttg
     9001 taattcagtt tctgcggcga cagctgcttg ttcgaatgcc agcgcttcac gctgagttcc
     9061 ccattcgcca ctgcccggca atgcggcggt tatcatcagc tctttatcgt tgacgcgacg
     9121 ctgtaattcc gccagttctt cggtggttgc tacaaaccag ctaccacgtc cggctaattg
     9181 tagcgcatcg ccgtcaacaa cttgattaac gtctgctttt ttgaggcgct cagcaacaat
     9241 ctgattaaac aacgcactgc gggctgccga caaccaaaaa ctccgtttat tgcgatcgcg
     9301 caccggagta ttggtttgcg cccagcgcag cgcgccctgc aagttgctac cgccaatccc
     9361 aaaacgttgg gcaccgaagt agttcggtac accttttgcg caaatatcga tcagacgttg
     9421 ttcaacgtca tcgcgattgc tcacttcgcg cagaaccagg gtaaaggcgt tacctttcag
     9481 cgcccctaaa cgcagcttgc gcttgtgccg cgcatactcc agcacctggc agccttccag
     9541 ttgaaaggcg ctcagatcgg gcatttcctt gcccggcacg cgagcacata accactgttc
     9601 cgttacagca tgtttgtctt tttgcccagc gaagctgact tcacgggaat gaattttcag
     9661 gaatttcgcc agtgcatccg ccacaaaacg ggtattgcag ccgtttttga ggattctaac
     9721 cagaatatgc tcaccttcac catcaggctc aaagcccaaa tcttccacca ccacaaagtc
     9781 ttccggattg gctttcagca gcccggtgcc ttgcggttta ccgtggaggt aagtgagatt
     9841 atcaaactca atcattttgt tgccttaatg agtagcgcca ccgcttcaca ggcaatccct
     9901 tccccacgtc cggtaaatcc aagtttttcc gtagtagtgg ctttcacgtt aacatcatcc
     9961 atatggcagc cgagatcttc ggcaataaac acgcgcattt gtggaatgtg cggcaacatc
    10021 ttcggtgcct gagcgatgat agtgacatcg acgttgccaa gggtataacc cttcgcctga
    10081 atacgacgcc aggcttcgcg tagcagctcg cggctgtcgg cacctttaaa tgccggatcg
    10141 gtatccggga acagtttgcc gatatccccc agcgccgccg cgccaagcaa tgcatcggtc
    10201 aacgcatgaa gcgccacgtc gccatcagaa tgcgccagca atcctttttc gtaaggaatg
    10261 cgtacgccac caatgataat tgggccttca ccgccaaagg cgtgtacgtc aaaaccgtgt
    10321 ccaattcgca ttatgtattc tcctgatgga tggttcgggt gaggtaaaac tcggccagtg
    10381 ccaaatcttc cgggcgcgtg actttaatgt tatccgcgcg gccttcgacc aactgcggat
    10441 gaaatccgca atattccagc gccgaggctt cgtcggtaat ggtagcacct tcatttagag
    10501 cgcgcgtcag acagtcatgt aacagctcac gagggaaaaa ttgcggcgtc agcgcgtgcc
    10561 ataagccgtt gcgatcaacg gtatgagcaa tggcattttt gcccggttcg gcacgtttca
    10621 tagtatcgcg cactggtgcg gcgaggatcc ctcccgtgcg gctggtttcg ctcaacgcca
    10681 acaatcgcgc gaggtcatcc tgatgcaaac aaggacgagc ggcgtcatgc accaataccc
    10741 actgcacatc gccagcggct ttcagacctg ccagcacgga atcggcacgc tcatcaccgc
    10801 catctacaac ggtgatttgc ggatgattcg ccagaggaag ttgtgcaaaa cggctatcgc
    10861 caggacttat ggcaatgacg acacgtgtca cccggggatg cgccagcagc gcatgcaccg
    10921 agtgttcaag aatggtttga ttaccgattg agagatattg cttaggacat tccgtttgca
    10981 ttcgacggcc aaatccggcc gccggaacca cggcgcaaac atccaaatga gtggttgcca
    11041 tgttaattcc cgggctgatt tatcgattgt tttgccccgc agactgtgcg cgcttcgacg
    11101 cgtcaggcac cagacgataa aaagtttcgc ccggcctggt catgctgagt tcattacgcg
    11161 cacgctcttc gagcgcctcc tggccgccat tgagatcgtc aatttcggca aaaagttgat
    11221 cgtttcgcgc tttaagtttc gcgtttgtag cttgcagtgc cgccacatca tcattgacgc
    11281 gggtatagtc atgtataccg ttcttaccga accacagcga atactgtagc cagaccagaa
    11341 tagccagcaa cagcagcgtt agtttaccca tcctgccccc tgaaaaactg catcatcatc
    11401 ccatgcatcc gaagacgact ctacatcctc tgttggggat accgcgacaa cgcgggcaaa
    11461 tgtaccacat ttgtccattg ttacgtatac ccagggcgtg cagaacataa tctcattatt
    11521 agttacggtt tgaattatga acagaggaga caagaaagta caaattagcc cagtagccac
    11581 ataaacagtg cgccaaacat aatgcctact gtcaccaggg tgaaaacaat actgtagcgt
    11641 agctttccgt ccatcaatga atgcagcgca atccccacca ctaccgcgac aggcatcagt
    11701 gccagaaaga aaggccaggt gtagataaag aagaacagcg tgttagagcc ataaatcaac
    11761 atcggcatcg ccagcgcaaa taaccaggag ataaaaccga ccacggcacc aggcagtgac
    11821 catgtagttt cttcatcctc agtaaggctg tcgttatttg ttagtgtaat gttatggcta
    11881 ttacgcatat ttgatcctgt tactttgacg aaccgggcat tgaaacccgg tggtgtctca
    11941 ggatctgata atatcgttct gtctcaacag atctaataat tgctgtacca aatttgttac
    12001 taattgttca ccattgagat gaatttctgc cgattcaggc tcttcgtaaa cggaatctat
    12061 tcccgtaaag ttgcgcagtt caccggcacg cgctttctta tataagcctt ttggatcgcg
    12121 ggcttcgcaa atcgccagcg gcgtatcgac aaacacttca ataaagcgcc cttctcctac
    12181 gcgttcgcga accatctggc gttcggcgcg gtgtggcgag ataaatgcgg tcagcaccac
    12241 cagtccggct gcaaccatca aattcgccac ttcaccgacg cgacggatat tctctttacg
    12301 atcggcatcg ctaaaaccga gatcgctgca taatccgtgg cgaacattgt cgccatccag
    12361 cagatacgta ctgacgccga gtttatgtaa cgcctcctcc agcgccccgg cgaccgttga
    12421 tttaccggac ccggagaggc cggtaaacca cagcactaca ccacgatgac cgtggtgtag
    12481 ttcgcgttgt tgcacagtga ccggatgact atgccagacg acgttttcgt catgcagcgc
    12541 cattatttat cccccagcaa atcgcgcgcg ccccagtgcg gaaagtggcg acgaaccagg
    12601 gcattcaatt ccagttcgaa tgcactgaat tcagatgacg cagctgttgc ctggctaact
    12661 ggctcgtgca ccataccggc acccacggtc acattgctca ggcgatcgat aaaaatcagc
    12721 ccaccggtaa ccggattttg ctgatagcga tctaacacca gtggctcgtc aaaagtgaga
    12781 tccaccaggc cgatgccgtt cagcggcagg ttttcaactt cgcgttgggt aaggttatta
    12841 atatcaacct gataacgaat gccatcaaca cgagcacgcg tcttcttacc ggcaattttg
    12901 atgtcataac tctggcccgg ggaaagcggc tgttccgcca tccataccac atccaccgac
    12961 gcactctgca ccgccggtaa cgcttcgtct gccgccagca gcagatcacc acggctgata
    13021 tcgatctcat ccgtcagcac cagggtgatc gcttctccgg caaagacttc ttcgcgatca
    13081 ccatcaaaag tcacgatccg cgcgacgttt gattccacac cagagggcag cacttttaca
    13141 cgttgcccga cttccacgcg accggatgcc agcattccgg cgtagccacg aaaatcgaga
    13201 tttgggcggt taacgtactg caccgggaag cgcattggct gggcatccac cactcgctgg
    13261 atctccacgg tttccagcac ttcgagcagt gtcagaccgc tgtaccacgg catactttca
    13321 ctttgcgaag ccacgttgtc accttccagt gcggagagcg gcacaaagcg gatatccaga
    13381 ttacccggca gctgcccggc aaaggtcaga taatcttcac gaatacgggt gaacgtctct
    13441 tcactgtaat ccaccagatc cattttgttg atcgccacga ccagatgttt gatccccaac
    13501 agtgtggaga taaaactgtg acgacgggtt tgatcgagca cgcctttacg ggcatcaatc
    13561 agtaagatcg ccagttcaca tgtcgatgcg ccagtcgcca tattgcgagt gtactgctcg
    13621 tgccctgggg tgtcggcgat aataaattta cgcttctcgg tagagaaata gcggtaggcc
    13681 acatcaatag tgatgccctg ttcgcgctca gcttgcaggc cgtccaccag cagagccaga
    13741 tccagctttt cgccctgggt gccgtgacgc ttactgtcgt tatgcagcga tgagagctga
    13801 tcttcgtaga tttggcgggt atcgtgcagc agacgaccaa tcagggtact tttgccgtca
    13861 tcgacgctac cacaggtcag aaaacgcagc aggcttttat gttgttgcgc aatcatccag
    13921 gcttcgacgc cgccttcatt ggcgatttgt tgtgcaagta cggtgttcat cttaaaaata
    13981 cccctgacgt tttttcagct ccatggatcc cgcctggtcg cggtcaatca cgcgaccctg
    14041 acgttcactg gtggtggaaa ccagcatctc ttcgatgatt tccggcagtg tttgtgcatt
    14101 tgactccacc gcaccggtca gcggccagca gcccagcgta cggaaacgca ccatccgttt
    14161 tttgatcact tcgcccggtt gcaggtcgat acggttgtca tcaatcatca tcaacatacc
    14221 gtcgcgttcc agaaccggac gttccgcagc gagatacagc ggcacaatgt cgatattttc
    14281 cagccagatg tattgccaga tatcctgctc ggtccagtta gagagcggga agacgcggat
    14341 gctttcgcct ttgttaattt gcccgttgta gttgtgccac agctccgggc gctgattttt
    14401 cggatcccag cgatggaagc ggtcacggaa agagtagatt cgctctttag cgcgggattt
    14461 ctcttcgtca cggcgcgcac caccgaaggc ggcatcaaaa ccgtatttgt tcagcgcctg
    14521 tttcaggcct tcagttttca taatatcggt atgtttcgcg ctgccgtgca cgaatggatt
    14581 aatccccatc gccacgcctt ccgggttttt atgcaccagc agctcgcagc cgtaggcttt
    14641 cgccgtacga tcgcggaact catacatctc gcggaatttc cagccggtat cgacatgcag
    14701 caacgggaaa ggcagcgtac ctggataaaa cgccttgcgc gccagatgca gcatgacgct
    14761 ggaatcttta ccgatagagt agagcatcac cggatttgag aattctgccg ccacctcgcg
    14821 aataatgtgg atgctttccg cctccagttg ccgcaggtga gtaagtcgtt tttgatccat
    14881 aaccgttcct ttgcaatacc gctattttct tgccatcaga tgtttcgact atagggagcg
    14941 taagagaacg aatgaaatta ccaattagaa tgagtagttc cttaacggaa taacgatttg
    15001 gcaaagctaa tatcaaaaag tgcttaaggc accggatttc gggcgtttag gaagatttga
    15061 aattgtttta gcgcagcggc agtttcatac tatggcggta aaaaaatttg catggtattt
    15121 aaggactcac tatgttttcc gcattgcgcc accgtaccgc tgccctggcg ctcggcgtat
    15181 gctttattct ccccgtacac gcctcgtcac ctaaacctgg cgattttgct aatacccagg
    15241 cacgacatat tgctactttc tttccgggac gcatgaccgg aactccagca gaaatgttat
    15301 ctgccgatta tattcgccaa cagtttcagc aaatgggtta tcgcagtgat attcggacat
    15361 ttaatagtcg gtatatttat accgcccgcg ataatcgcaa gaactggcat aacgtgacag
    15421 gaagtacggt gattgccgct catgaaggca aagcgccgca gcagatcatc attatggcgc
    15481 atctggatac ttacgccccg ctgagcgatg ctgacgcaga tgccaatctc ggcggactga
    15541 cgttacaagg aatagatgat aacgccgcag gtttaggtgt catgctggaa ctggcagaac
    15601 gcctgaaaaa tacgcctacc gagtatggta ttcgatttgt ggcgaccagc ggcgaagagg
    15661 aaggaaaatt aggcgctgag aatttactca agcggatgag tgacaccgaa aagaaaaata
    15721 cgctgctggt gattaatctc gataacttaa ttgttggcga taaattgtat ttcaacagcg
    15781 gtgtaaaaac ccctgaagca gtaaggaaat taacgcgcga cagggcgctg gcaattgcac
    15841 gcagtcacgg aatagccgca acgaccaatc cgggtttgaa taaaaattat ccgaaaggca
    15901 ctgggtgttg taatgacgca gaaatattcg acaaagcggg cattgctgta ctttcggtgg
    15961 aagcgactaa ctggaatctt ggaaataagg atggttatca gcaacgcgca aaaacacctg
    16021 ccttcccggc gggaaatagc tggcatgacg taagactgga taatcaacaa catattgata
    16081 aggcacttcc aggaagaata gaacgtcgct gccgtgacgt tatgcggata atgctaccgt
    16141 tggtgaagga gctggcgaag gcgtcttgat gggtttgaaa atgggagctg ggagttctac
    16201 cgcaggggcg gggaattcta agtgataccc atcatcgcat ccagtgcgtc cggtttactt
    16261 ccgggaaaac cgcaccgtat tgcgccggtt tatctccgct ggagcgggga acacctctgg
    16321 cgaatagaaa gcaagccagg ttttgaccag tcgatcgtac atggcattgg ccgttgccat
    16381 tgttggcagg ccaaaaaaca gcccctgagc gttacccgca gccatcaacc tgtgcgccag
    16441 gataagcgcc gcttctgttt tacctgcgcc ggtcacatct tccagaataa acaactgtgg
    16501 tcctggctga ttgatatcca gatccagcac cttttgctgt aacggtgtcg tattctcaat
    16561 aaaaggaaac agcgtattaa ttccggtgaa aggcgcggtt tctgcttgtg gcggaaagac
    16621 ggttaatgcg ttttgagcct gaactaaagc cttttgccag taatcttcaa tatctattgc
    16681 ctgtgcgacg cgtggaaaaa atcgcgttga cgaccctgtc cagtctgcaa gtacgacggt
    16741 tgcagagata taccaggaaa gttgttttat aagttcaacg ccctcgtcat catcccagaa
    16801 tgtggggatc tctattcgcg gaaacagtgg cttgatttca aggagaaaat ctcgtgctgc
    16861 agctttgtct tcaggcagaa aattatccaa ttcatccata cgatcagggg gccgaccatg
    16921 atgccctgtc gtcacgggca tccacatttc tatcactcgc ctaagtttgc gcggagagag
    16981 cgaagatgaa ggaagtaact cctgacattc acttaaataa tgattccaca accagtagcc
    17041 cagcgttgaa tgagagattt tttcgtaatt ttttctggca ccttcctgaa tcttgaattc
    17101 agggggcagg taaagttgtt gaaaagagcg ggcaaatttt ccaatatcgt gccagcacag
    17161 caaccaggcg aaaaattgag ctgcctgttc cttgtcagaa atccctaatt gacgaaaata
    17221 atcagccagc ccgaagcaat ttcttttaac cattaaatag cccattgcgg ccacatccag
    17281 caaatgccag caaagaaggt gatagtcgtc gctaccctct ttctcgccac gtcggttttt
    17341 tccccagaaa tcaaagaaag tcacaatatt tttatccttc agtaaactta aaggatattt
    17401 acgcatattt taaaaactat ctgtgatata tatcagccaa taaaacattt acattatgaa
    17461 taaattaatg attttcattt gaaattcata ataataaata gagactatat attcacataa
    17521 aaaatataaa ttttaaaaaa gggtgcagga gaccaaacca tcacaattca gtacgcgggg
    17581 ttttaccccc gcgtggtggc tacttcttcg attcgtaagc gagaactgcc ttaaactcca
    17641 taccacgttc tctgatactc aactcacata acgagtcacc taccatcaac gtgaatccca
    17701 gtactgtaca acacagtacg atgattgcca caagggcata ttttgtcagc atgtcttgcc
    17761 tccctgcaaa gaagagacta acatccgaat tgctacgttc gaacgcgagc ctcaggttga
    17821 tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta acgccagaca
    17881 gcctcaagca cccaacgcca ttctatacct gttaattttc cctgcgatgt tcacctttaa
    17941 atgccggaat gctgctcgca aaataaaaac gcccccatca tttctgacag aggcgtttaa
    18001 tttatccggc aatatttacc cttcgtgtaa tccacattcc ctttttaagc caaagaaacg
    18061 cgtttcttct tccaccatgc cgggttccca tttacgggtt gtatgggtat cgcccaccga
    18121 taaatatcct tcgtcccata atgggtgata tttcaggcca tgtttttgca ggtactgata
    18181 aatagttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac gctgaattgc
    18241 cagcaccggt aaattggcac ggctgccgga ttgttcacgc cgcaggccag caaaccacgt
    18301 ttgcgcattc agttctttca gcgcccggtt catcggttcg actttgttga tgtcattgta
    18361 cttttcaatg ccttcaacgc cctgttccca cagtttgccg tagcgtgctt cctgccaggc
    18421 ggcgctttcg gtagcgcggt acactttcag gttgagcttg agtttgtccg ttaactcgtc
    18481 aataaagcgg taggtttccg ggaacagata gccggtgtcg gtgaggatca ccggaatatc
    18541 cgggtgaatc tgattcacca gatgcaggct caccgccgcc tgaatgccaa agctggaaga
    18601 aagtacatat tcaccgggca gattatccag cgcccaggca acgcggcctt cagcgtccag
    18661 tttttccagt tcggcgttag tttctgccag cgccagaatg cgatctacct tcggcagttc
    18721 gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct tgcctgatgc
    18781 gacgctcacg cgtcttatca ggcctacaag accgggctga tggttaatcc cacaaatcgc
    18841 gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg ccgaagcctt
    18901 cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac gccaggattt
    18961 ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaatg cggttgccgc
    19021 caagatgcag gttgtagcga cccggtgctt tacccaccag gcccacttcc gccagcatcg
    19081 cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca tcgctgacac
    19141 catgtttcgc cattaaatta tcgatgttgt cgataaaaga cggcaggaaa cgctccgctt
    19201 ccgccatcgc cagcgggcaa gtcgggaatg acacgcaggc catcgagttt tcacgctgcg
    19261 gcgtgacggc attcattaac ccgctctctt tggcaatttt ctcgatcttc gctttctcgc
    19321 tttccggcac accggcgatg atcagattct ggttcgccgt aatgcggaaa tcacctttgt
    19381 ggatcttcgc gatctccagc aggccagttt tcagcggacg ccccggataa tcaaggatgc
    19441 gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta acccagccaa
    19501 tacgatcgcc gcgtcctgta aattcatacg ggcggatcgg ttcaaatttg atccccgcgc
    19561 gacgctccac ttccgcttta aacgtctcaa cccccacgcg ctccagtgtg tattttgttt
    19621 tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg acggcttccg
    19681 ccaccgctag cgtatgctct agcggcagat agccaaactc actcgccgtg cgggcgtagg
    19741 ttttcttgtt accgtgttcg atagaaagcc caccgcccac cagcaggttg aagcccacca
    19801 gcttgccgtt ttccgcgatc gccacgaagt tcatgtcgtt ggcgtgcaga tcaatatcgt
    19861 tctgcggcgg gatcactacc gtggttttga acttacgcgg caggtaggtc tgaccaagga
    19921 tcggttcttc atcggtggtg gcgacttttt cctgatcgag ccagatctcc gcatacgcgc
    19981 gggtacgcgg cagcagatgt tcagaaatct tcttcgccca ctcgtacgct tctgcgtgca
    20041 gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg ttagcagtcg
    20101 ccagcgcatc aagaccgacc gagtgcagca tctggtgcac cggtttgacg ttctttttca
    20161 gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg aatgctgcca tagatggtgt
    20221 tttcaccggc aaatttatcg atcgcctgcc actgtttggt ggtgataact ccacccggca
    20281 gacggcagcg caacagcatc gcgtggcgcg gctccagctt ctgttcagca cgttcggcgc
    20341 ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg aagttatcgc
    20401 ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc aatggtgccg cgcaggtagt
    20461 tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact aaaggccctg
    20521 gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc gcagctcact
    20581 taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca cttccagaag
    20641 tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg cagacataaa tgtgggcacc
    20701 gtcattgatc cagcgccaca gctccgcgcc ctgttcgcgc agtttatctt gtacgtaaac
    20761 tttttctttt tggtcgcgcg accaggcgag atcgatccgc gtcagcacac cctctttgac
    20821 gtagcgctgc cactccacct gatacaggaa gtcttccgta aaatgcggat tgccaaagaa
    20881 cagccagttt ttacctggcg cttcgtcagc ggcgcgttgc tgcataaagg cgcggaacgg
    20941 cgcaataccg gtgcctgggc caatcataat caccggggtt tccaggttag cgggcaggcg
    21001 gaagttatcg ttatgttcga taaatacgcg gacttcgccc tcttcttcca cgcggtcagc
    21061 gaggaagctg gaggcaccac cggcacgggc gcggccttcc acgtcgtaac gcaccacacc
    21121 aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag gaggcaatgg aatagagacg
    21181 cggggtcagc gggcgcagca gattaattag cgcttcggca tcaagctgtg ccggggagaa
    21241 acgcaccatg tcaacaatca gcgtcgtcgc ggcgtaatgc tgtaacttcg ctttatcgcc
    21301 caccagcggc agcagtgttt cactgcgggt aagcgtggcg taattctcaa caatgttggc
    21361 ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca acgttttgcc
    21421 ctcgacagtg acaggttcat cgcctgtcag ccacagcagt tcgacaagtt ctttcaccag
    21481 tgccggatcg ttctgatacc agacgcccag cgcgtcaccc ggctggtaac gcaggcccga
    21541 gtcgcctaag tcaatttcga tatggcgaac gtctttttca gagttacgcc cagtaatttt
    21601 ctggttaaca gagaggctag ccaccagcgg cgcgtctttg ctgtacgggc tggtgtggat
    21661 ttcatttacc gcgccagtag cgacggattg cgaaggtgcc gcgacaggcg cacgcgattt
    21721 aagcgcatca accacgcggg cgcgccactc gctggcagca gcctggtatt caacatcaga
    21781 atcgacacgg tcgagcaggc gttcaccacc cagttccgcc agcttgctgt caaaatcttt
    21841 cccggactgg cagaaaaatt cataagagct atcgccgagg ctaaacacgg caaacgcggt
    21901 gttttccagc tttggcgctt ttttggagaa caggaactta tgcagcgcga cggcttcttc
    21961 cggcggttcc ccttcccctt gcgttgacgt cactacgatg agcagttttt cgctggcgat
    22021 ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg ctgctaataa
    22081 atcatcgcgt aatgcttcag caacccggcg cgcattgccg gtttgcgaag cagagataat
    22141 cgttataccc ggcatttctg cggcgggcgc tggcgtcact gcaagcgcaa caggctgctg
    22201 gttgagcacg ccccagaaat agccagaaac ccaggcaagc tgggtaggcg ttaaatcggt
    22261 cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac ggaagcaacg cggaaggtgg
    22321 gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa gctgtttctg cgccctgtca
    22381 gcgcccataa aacagaagag aaggtaaggt taacggggtg aacggtgtgg attaaagacg
    22441 ggatagcgat aactaataac caaatcgact aacctgtttt agcaatagtc tttaacaaca
    22501 aaatagatta accaacctaa tgaaaaacaa atgaatttag ccaatcatta agataaatca
    22561 gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa aacagccttt ccggtactct
    22621 acggcggttt tatcgtcctg tagagaaatt atgatgtcca ccacgttatt taaagatttc
    22681 accttcgaag ccgctcaccg cttaccacat gtcccggaag ggcataaatg tggtcgcctg
    22741 cacgggcatt cctttatggt gcgactggaa attaccgggg aagtcgatcc gcatacgggc
    22801 tggattatcg atttcgctga actgaaagcg gcgtttaagc caacttatga gcgcctagat
    22861 caccattatc tcaatgatat tccaggtctg gaaaacccaa ccagcgaagt tttagcaaaa
    22921 tggatttggg atcaggttaa acccgttgtg ccgctgttaa gtgcggtgat ggtaaaagaa
    22981 acctgcaccg cagggtgtat ctatcgcggc gaatgataag agtgtgtcgg cggtcaattt
    23041 ccctttagta acgctatgtt agggtgttgt gttctggata tctggggcat gacatggaag
    23101 acgactgcga cattattatt attggtgccg gtattgcagg caccgcttgc gcgttacgct
    23161 gcgcgcgagc ggatttatcc gttttgttac tcgaacgcgc tgaaatcccc ggcagcaaaa
    23221 atctttccgg caggcggtta tatacccatg cactcgcgga actcctcccg caatttcatc
    23281 tgaccgcgcc tcttgaacga cgcatcactc acgaaagcct ttccctgtta acgccggatg
    23341 gcgcaacgac gttttccagc ttacagcccg gcggtgaatc ctggagtgta ttacgtgcac
    23401 ggttcgatcc gtggctggtt gccgaagccg aaaaagaagg tgtcgaatgc atccccggag
    23461 cgacggtgga tgcactgtat gaagaaaacg gcagagtctg tggcgttatt tgtggtgacg
    23521 atattctccg cgcccgttat gtggtgctgg cagaaggtgc caacagcgtc ctggctgaac
    23581 gtcacgggtt agtgactcgt cctgctggcg aagcgatggc gttggggatc aaagaagtgc
    23641 tgtcgctgga aacatccgct attgaagaac gttttcatct ggagaataac gaaggcgcag
    23701 cgttgctgtt cagcggcgga atctgtgatg acttacccgg cggcgcattt ctttatacta
    23761 atcaacaaac gctctcgtta gggattgttt gcccgctctc ttcccttacg caaagtcgtg
    23821 ttccggcaag cgagctgctg actcgcttta aagcgcatcc ggcagtgcgc acgcttatca
    23881 aaaacacgga atcactggag tatggtgcgc atctggtgcc agaaggtggc ttgcacagta
    23941 tgccggtgca atacgccggt aacggctggc tgctggtggg cgatgcgttg cgcagttgcg
    24001 tcaataccgg aatttccgtg cgcggcatgg atatggcgct gactggcgca caggcggcgg
    24061 cacaaacgct aataagcgcc tgccagcacc gcgagccgca aaatctgttt gcgctttatc
    24121 atcacaacgt agagcgcagc ctgctatggg atgttctaca acgttatcag catgttccgg
    24181 cgcttttgca acgccctggc tggtatcggg cgtggcctgc gttaatgcag gatatttccc
    24241 gcgatttatg ggatcagggt gataaacctg ttccaccgct gcgccagtta ttctggcgtc
    24301 atttacgtcg tcatggcctg tggcatctgg cgggcgatgt tatcaggagt ctgcgatgtc
    24361 tgtagcccgt aatctctggc gcgttgctga tgcgccgcac attgttccgg ctgactccgt
    24421 tgagcgtcag atggtacaac ggttgattaa cgcctgtccg gtaggtcttt tttctctcac
    24481 acaggaaggt ggcttacgta ttgactatcg cggctgcctg gagtgtggca cctgccgttt
    24541 gctgtgcgac gaatccacac tacaacagtg gcgctatccg ccttccggat tcggcatcac
    24601 ctaccgcttt ggataaataa taaggataat ttatgcccct cttacacctg ctccgccaga
    24661 atccggtgat tgctgccgtt aaagataatg ccagcctgca actggccatc gattctgaat
    24721 gccagtttat ttccgtgttg tacggcaata tctgcaccat cagtaatatc gttaaaaaga
    24781 ttaagaacgc cgggaaatat gcttttattc atgttgattt gctggaaggc gcgtcaaata
    24841 aagaagtggt gattcagttt ttgaaactgg tgaccgaggc ggatggaatt atcagcacca
    24901 aagcatcgat gctaaaagcg gcaagagcgg aagttttttt ctgtattcat cgcctgttta
    24961 ttgttgattc gatttcgttt cacaacattg ataagcaagt tgcgcaatcg aatccggatt
    25021 gtattgagat cctgccaggc tgcatgccca aagtgctggg ctgggtgacg gaaaaaatcc
    25081 gccaaccgct gattgctggc gggctggtat gcgatgaaga agatgcgcgt aatgcgatta
    25141 acgcgggtgt cgtggcgctt tccaccacga ataccggagt ctggacgtta gcgaaaaaat
    25201 tgctttgacg ggataatcgt aaccaattga atttgttttg atttgttggc cgcacgccac
    25261 atccgacatt cagcgcctga tgcgacgctt aacgcgtctt atcaggccta caggtcccca
    25321 gtccagaacc gtaggcagga tcatccggca gcacgttatt ggtattctgc gtgaatgttt
    25381 gtcaccagtg cctccagcac cactttccag tcatcaacca cgtcaatatc tgcctgtgaa
    25441 aataccgctg cgctggcgtc atgattaatc gccaccataa acttgctatt gcgtacgccc
    25501 gccattaacg ctgctgcgcc ggaagcgcca acaacaatgc acacctcagg tgccagcaga
    25561 tgtccggaaa taccaatcac tttttcagca tcaacgccac cgttcatcac tcgcgcccga
    25621 ctgtagccca cttcggttcc cagcttttct gccagcataa cgatttcctg gttatctgcc
    25681 tctccgccct gccccaccac cagcacccgt ctggcctcag ctaatgggtc gcggctaaca
    25741 tttttcagat cctctgtgct aaccagccag tcgggaagcg cacctgggac aatgttcagt
    25801 tgctgcattc cagagggcaa cgtggcgttt ttcgctgctc ccgcctgacg cgccagggaa
    25861 aggcataacg ggcgtttttc tgtttgcagc gtcgccgtta acgcatttcc ccagtgggat
    25921 ttgcgcatgc ttacggttgg aatatctaac gaagtcacct gacagatgct tgcaccatgt
    25981 aaacgccagg ccagccgggt cgacagttca tcgccaaacg ttcccggcgg aaagagaaca
    26041 acgtccgcag gcgttcgctg ccactgttcc accatcgcat ccagcacctg ttccgccact
    26101 acgggctgag gttctatttg ccagtgcgcc agcgtacaac cgctgaaatc ctgcgccgcc
    26161 agccagctcg ctattgcggc attctcctga tttattgtca caattgcgat attcatggct
    26221 gcatcctttg gcgtaaatag tcctgccata gcttttgagc tttttctgct accgtttgcc
    26281 catcaatcag cgtcgctccg catcgttgct ctgctcttgc cagttgcaga cactgcatcg
    26341 ctggcatttc ggcagcaaca gttttacgga taatttctgc tttccccgca gccattcgct
    26401 ggcgcattcc cggtactggc aaggccactt caccgcactg acgcacggca atcaccgcag
    26461 gcaagcgaac ccggcaacag cgcaacccat gttcagtacg ctgttcgagg gtgataaaca
    26521 gcgcgtcgag agtgaaacgc tccaccaggg tgaagcaggg ccagcccagc atttccgcca
    26581 gcaaaaatgg cgtttgccca ttctgccctt cgctgctttg gcagccggta attatcagat
    26641 ccagcggatt ctgacgctgc cattcggcaa tatggcgagc gacaaattcc ggcgcaaagc
    26701 gcagatctgc cgccgtctcc agcaatacag cttcctcaaa cccaagagcc atgagatagc
    26761 gcagccagtg caacgcccgt tcatccccca tgctcaacgc ggttaaagac atcggcgtac
    26821 cgttttttct ctgcgctaac agcagcgcgg cagcggcctg ttcatcagca ccgagtaaac
    26881 ttcgcagtag cgaaacatcc ggtccgctat taccctgagc cgccgcctgc cactcttttt
    26941 ccgccagcat tccggcatcc ggttcggctt taaacgctaa cagaatgttc attcgcacct
    27001 cctacatttc ccgccgccac cagcgggagg gctttagttt ctggtgccca tagccaggtc
    27061 acaacaaaac caaccaacaa cactgtcgcc aggagcaata aggtgacttg cattccccac
    27121 tgcgccagca cccacggcag caggccagta cttaccgccg cgccaagtcg actcatggcg
    27181 gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa aactttccgc aggcaaaatg
    27241 cccaccagat tactgactgc cgaaatggtg gtgctgaaga gaacaaaaag cagcagcgtt
    27301 aatgaactgc cggaaggcaa acaggccatt actaccagcg ttgccgccag cagcaaaaaa
    27361 cttcccagca aaaatttgcg atgtgccagc aggtgcgtca gaactaatcc cagcagcgcg
    27421 cccacaatta acaacgcatt aagcatcagg ctggcagtca gcgcatcttc cagaccaata
    27481 gtctgggcga tagttggcag ccaggtataa atcacaaacc atgggattac gaggcagaca
    27541 aagaagatgc tgttaaacgc cgtgcgccgc cagtaacgcg aagagaacaa ggttttgatg
    27601 tgtttatggg tcgccgttac cacttcatcg cccagtaaaa catggggacc aaaatagcga
    27661 tgcacgatag cgtgagcttc tgcaaaacgc ccctggcaca gtagccaacg tggcgattct
    27721 ggcgttcccc agcgtaataa cgtaatcaac aacgcgggca gagctgccga tgccagcaac
    27781 cagcgccagg cctccgggtt ttcggaaata aagtgatgtc cggcaatact tgccaacaca
    27841 tagcctacgg tccacaccac gctgaatgcg cccagcaaaa taccgcgatg gcggcgcggg
    27901 gaaaattcag ccagcaaggt gtgacctact gagtaatcgc ctcccagacc aatgccgatc
    27961 aaaatgcgca gcccaataag atgctctggc gtggtggcaa aaaattgcaa gaacgaagcg
    28021 agcgtaatca gcaaaaagct gaaggtgaag attttttgcc gaccaatatg gtcggagatc
    28081 cacccaagaa ccaggctgcc aagaaacaaa ccaaggagcg ccgagccgcc gctcattccc
    28141 gccataaacg gcgtcagttg catggcgggc gtaagttgaa taatggcgta accaatgacg
    28201 ccgagaacat aaccgtcggt caggtgtgcg ccgaaagtga gcgcagcaat tcgacagtga
    28261 aaacggttaa gcggtaaatc atccattcgc accggtgaag tgttcatctt taccccttca
    28321 ttgtccggta ataagaacgg cttctcgcct gagaagccga aaaggcaacg ccccgtcatc
    28381 gcggaacgct gcctgataca ttatttttca atcggataga tagtacccgt attcataatg
    28441 ccgttaggat cgaactgctt tttcagccct tccagcaacg cccacgcgct gccgtgttcc
    28501 agcttgctcc agtgaacgcg atgtttacca atgccatggt ggtgcaccat cgaaccgccg
    28561 aggcgaatgg tttcttcgca gatgattttg ttgagcggat tgtggtactt gtcgatttcc
    28621 tcttccggct tacagtcaac aacgttgtag tcatagacga agtacatgtt ggtgccgttc
    28681 tgatagctat gagaggagtg accgcccagc atggtgatgt cgtcggcgtg cgggaactca
    28741 gtacgaatac ggttaataac gctttcgtag atttcgtgga tgcagctcca gcagccggac
    28801 acttcggtgg taaagcccat gttgccggtt ttgaggatct gcacacgttc ggcagccact
    28861 ttatccggtc cccagttcag gttgttaaac caggtttcaa tcagtttgct gtccacgcgc
    28921 tggcactgcg ggtagcgggc aacgatttcc gcaatccctt cgcccgtcgc cttcgcaatg
    28981 cgagggttac cttcagccat aaagatcagc acccattttc cgtcggcaaa atgggtgaag
    29041 tgttgggtgc catcttcagc gtcatacaaa cgagcgatcg acggacgata cccttccacc
    29101 atgatttcac gcaggatgtt gaagccggtt ttcatgtctt ccaggatata gccgtagaag
    29161 aggttgtttt ccggggtgta tttaaagatt ttcactgtta cttcagtgat atagcacaat
    29221 gcaccttcgt tgccgatgat gatgtgacga atgtccgggc cagccgcgcg gcgtggcacg
    29281 tttttaatgc gtgtgacggt gccatctgcc aacactgcct ccagaccaac gaccatatct
    29341 tcgattgcgc cgtagagtgt ggagaactgc ccgatactgc gggttgctac caggccgccc
    29401 atctgcgcca gcggctttga ctgcggagaa tgccccgtgg tgtaaccttt ttcacgcaac
    29461 gcgttttcca gcacttccag cggaacacca cattgcgccg tcgcctgcat attctcaata
    29521 tcaatattaa tgatttgatt catggcggag ccgtcgagca ccaccgagtt ttctacaaca
    29581 gtttccagcc caccttcggt ggcggaagca ccggtacgcg gcacaccgtt aattttgtgc
    29641 gcattcataa aattcagcac acgggatact tactctgtgg aaccgagttt tacgaccgct
    29701 gccggaatcg gcaaagtata aatgccatga atatccggaa atttacgaaa acggtcaata
    29761 ctgtttttct ttaatactgt ttcatcggta attacgcgat ctgcaccaac aatttccttt
    29821 agctggtcga caatcgctgc gcgagataaa gacatagtaa ttccttcctg ataataaaaa
    29881 tggaataatc attaataaga gaaaacagaa accaccgtta tggattagcg cactaaataa
    29941 ccgccatcga ccactaataa atgcccgttg acataattcg atgcctgact tgcgagaaac
    30001 acggctgcgc ccattaaatc ctgagtatcg ccccaacggt ttgccggaat atgatcaaga
    30061 acgcgctgat tggtttctgg attactgcgt gtcgccagca taatatgctg ttgcataata
    30121 gccaggggcg ataccattta cctgaatatt atattgacct agttcatcac aataagcttt
    30181 ggtgaacccg gcaagagcat gtttagtggc agaatatgca ggtgaccatt gtccacctaa
    30241 gtaagagaac aatgaacaga tattaataat tttgccgctt ttctgcggga tcataatttt
    30301 tgccgcttca tagcttaact cgaatgcggc ggtcaggttc acatcaatca tcggatccca
    30361 gtcggcacga ccgaagtcca gcaccttatt cagcttacaa ataccggcat tgttaaccag
    30421 aatatcgaca gtaccgaagc gctcacagca ggcagcgata atcttctgcg gtgcgccttc
    30481 tgcggtaata tccacctgca tgaagtccac ttcaacaccc tgtttttcaa tcatttcctt
    30541 tgtttcgccg ttatctttga cgaagctagg aataaaaata tttgcgccag ctttggccaa
    30601 cgccattgca aatgcctgac ctaaaccgct attcccgccg gtaacaattg ctgttttacc
    30661 tttcagggag aaaaaatcca ttgagaacgc attgagagat tcgattgaca tagttagctc
    30721 caatttccgt aaggcaaaaa aaaagaaagg taacttcccc cgcgagaggg aagttacctt
    30781 tctcattatc tctggtaact ggagataatt tttagcataa cggcataact gctaatgtga
    30841 tcgtaatcac agtgtgatat tcgtattgtc aaatagtttt aaaaagcaga agcgtgatta
    30901 ctctcacaca acacctctgc tttccatgtt atttcttatt gcaattacta gagacctgag
    30961 aaaagtaatc cacctgaaag ggcggcttac ttttctcttt tttttgtcta ttacaaatgg
    31021 gaatacgata tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat aattagtttt
    31081 agcggcggcg ttagttttga cctggcttat ttacgttata tttatcaaat tcccatggcg
    31141 aaatttatgg gattcagcaa taccgagata ggtttaataa tgagtacctt tggtattgcg
    31201 gctattattc tttatgcccc cagcggcgtt attgccgata aattttcaca ccgcaaaatg
    31261 attacttccg cgatgatcat taccggatta ctgggtctgt taatggcaac gtatccaccg
    31321 ctgtgggtaa tgctctgtat tcaggtcgcc tttgcgataa cgacgatttt aatgctgtgg
    31381 tcggtgtcga ttaaagccgc ctcgttgctt ggcgatcata gcgagcaagg gaaaattatg
    31441 ggctggatgg aagggctgcg cggcgtcggt gtaatgtcgc tggcggtgtt taccatgtgg
    31501 gtcttttctc gctttgcacc ggatgacagc gccagcctga aaacagtcat tatcatctac
    31561 agtgtggttt acatcttgtt ggggattctg tgctggtttt ttgttagcga taacaacaac
    31621 ctgcgcagtg ccaataacga agaaaaacag tcattccagc ttagcgacat ccttgccgtt
    31681 ttgcgtatca gcaccacctg gtattgcagc atggtgattt ttggtgtctt cacaatctac
    31741 gccattctga gttactccac caactatctg accgaaatgt acggcatgtc gctggtggca
    31801 gcgagctaca tggggattgt gatcaacaaa atcttccgcg cgctgtgcgg cccacttgga
    31861 ggcattatca ccacctacag taaagtgaaa tcccctaccc gcgtgattca aatcctttcc
    31921 atcatcggtt tgctggcgtt gacagccctg ctcgtcacga actctaaccc gcaatcggtc
    31981 gcgatgggga ttggcctgat tttactgctg ggattcacct gttacgcctc acgcgggctg
    32041 tactgggcct gccccggcga agcgagaaca ccgtcttaca ttatgggcac cacggtaggt
    32101 atttgttcgg tgattggatt cctgccggat gtcttcgttt acccgattat cggccactgg
    32161 caagacaccc tgcccgctgc agaagcctac cgcaatatgt ggctgatggg catggcggcg
    32221 cttgccatgg tgatagtctt tacctttttg ctgttccaaa aaattcgtac tgctgatagc
    32281 gcccccgcaa tggctagcag caagtaagcc aggaaaatta caggagaaaa atcatgtcga
    32341 aaaaatacat catagggatt gatggcggaa gtcagagcac aaaagtggtg atatacgatc
    32401 tggaaggtaa tgtggtttgc gaagggaaag gcttattgca gccgatgcac acgccagatg
    32461 ccgatactgc agaacatcct gacgacgatt tatgggcatc attatgtttt gccggtcacg
    32521 atttgatgag tcagtttgcc gggaataaag aagatattgt cggtattggt ctgggatcca
    32581 tccgttgctg ccgtgcgtta ttgaaagccg atggcacggc cgttgattag ctggcaggat
    32641 gcacgcgtaa cgcgtcccta cgaacacact aatcctgacg tggcatatgt cacctctttt
    32701 tcgggttatc tgacgcatcg cttaaccggc gagtttaaag acaatatcgc caactatttt
    32761 ggtcagtggc cggtggatta taagagctgg gcatggagcg aagatgctgc ggtaatggag
    32821 aagtttaata tcccccgtca tatactgttt gatgtgcaaa tgccaggcac tgttcttggg
    32881 catatcacac cacaagccgc gctggcgaca catttcccag ctgggctgcc ggttgtttgt
    32941 accaccagtg ataaaccggt agaagctctg ggggccggat tactggatga tgaaacggcg
    33001 gcgatttcac tcggtactta tatcgcgctg atgatgaacg gcaaagcctt gccgaaagat
    33061 ccggtggcgt actggccgat tatgtcttct attccgcaaa cattgctgta tgaaggttac
    33121 ggtattcgca aaggtatgtg gacggtgagc tggctgcgcg acatgttagg cgagtcgtta
    33181 attcaggatg ccagggcgca ggatctttca ccggaagatt tactcaacaa gaaagcctct
    33241 agtgtgccac ctggctgtaa tgggctgatg acggtgctgg attggctgac caatccgtgg
    33301 gaaccgtaca aacgcgggat tatgatcggc tttgattcca gcatggatta cgcatggata
    33361 tatcgttcga tactggaaag cgtggcgctg acgctgaaga acaattacga caatatgtgt
    33421 aatgaaatga atcactttgc gaagcatgtg atcattactg gcggcggttc gaacagcgat
    33481 ctgtttatgc agatttttgc cgacgtgttc aaccttccgg cacgacgtaa cgccattaac
    33541 ggttgtgcaa gcctgggggc agcgattaat acagcggtag gtctggggct atacccggat
    33601 tacgcaacgg ctgtcgacaa aatggttcgc gtgaaagata tctttatgcc tgttgagagc
    33661 aatgccaaac gctacgacgc gatgaataaa ggtattttca aagacctaac caaacatact
    33721 gatgtgatcc tgaaaaaatc gtatgaagtg atgcatgggg aattggggaa tgcggattcg
    33781 atccagagct ggtcgaatgc gtaagtgagg aagaccgggc gggaaactgc ccggcctgaa
    33841 tatacctgaa tggttatccc cgctgacgcg gggaactcga ataactgacc aacgtctggc
    33901 tctctaaatc cggtttatcc ccgctggcgc ggggaacact ctaaacataa cctattat
//
LOCUS       DAJTXY010000028        33495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTXY010000028 DAJTXY010000000
VERSION     DAJTXY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.028"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            535..672
                     /locus_tag="OO566_001481"
     CDS             535..672
                     /locus_tag="OO566_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604811.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1272..2051
                     /gene="ipaJ"
                     /locus_tag="OO566_001482"
     CDS             1272..2051
                     /gene="ipaJ"
                     /locus_tag="OO566_001482"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6604812.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2999..3928)
                     /gene="virB"
                     /locus_tag="OO566_001483"
     CDS             complement(2999..3928)
                     /gene="virB"
                     /locus_tag="OO566_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6604813.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4145..4381)
                     /locus_tag="OO566_001484"
     CDS             complement(4145..4381)
                     /locus_tag="OO566_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6604814.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="OO566_001485"
     CDS             complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="OO566_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6604815.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTSPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="OO566_001486"
     CDS             complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="OO566_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6604816.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="OO566_001487"
     CDS             complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="OO566_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6604817.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="OO566_001488"
     CDS             complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="OO566_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6604818.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINKTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="OO566_001489"
     CDS             complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="OO566_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6604819.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="OO566_001490"
     CDS             complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="OO566_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6604820.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="OO566_001491"
     CDS             complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="OO566_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6604821.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="OO566_001492"
     CDS             complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="OO566_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6604822.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13569..15185
                     /locus_tag="OO566_001493"
     CDS             13569..15185
                     /locus_tag="OO566_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6604823.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLPQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15195..15557
                     /gene="ipgE"
                     /locus_tag="OO566_001494"
     CDS             15195..15557
                     /gene="ipgE"
                     /locus_tag="OO566_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6604824.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15557..16015
                     /gene="ipgF"
                     /locus_tag="OO566_001495"
     CDS             15557..16015
                     /gene="ipgF"
                     /locus_tag="OO566_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6604825.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16024..17139
                     /gene="mxiG"
                     /locus_tag="OO566_001496"
     CDS             16024..17139
                     /gene="mxiG"
                     /locus_tag="OO566_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6604826.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17147..17398
                     /gene="mxiH"
                     /locus_tag="OO566_001497"
     CDS             17147..17398
                     /gene="mxiH"
                     /locus_tag="OO566_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6604827.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17411..17704
                     /gene="mxiI"
                     /locus_tag="OO566_001498"
     CDS             17411..17704
                     /gene="mxiI"
                     /locus_tag="OO566_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6604828.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17710..18435
                     /gene="mxiJ"
                     /locus_tag="OO566_001499"
     CDS             17710..18435
                     /gene="mxiJ"
                     /locus_tag="OO566_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6604829.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18432..18959
                     /locus_tag="OO566_001500"
     CDS             18432..18959
                     /locus_tag="OO566_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6604830.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18901..19596
                     /gene="mxiN"
                     /locus_tag="OO566_001501"
     CDS             18901..19596
                     /gene="mxiN"
                     /locus_tag="OO566_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6604831.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKR
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19589..19996
                     /gene="mxiM"
                     /locus_tag="OO566_001502"
     CDS             19589..19996
                     /gene="mxiM"
                     /locus_tag="OO566_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6604832.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19980..20408
                     /gene="mxiM"
                     /locus_tag="OO566_001503"
     CDS             19980..20408
                     /gene="mxiM"
                     /locus_tag="OO566_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6604833.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20538..21170
                     /gene="mxiE"
                     /locus_tag="OO566_001504"
     CDS             20538..21170
                     /gene="mxiE"
                     /locus_tag="OO566_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6604834.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21157..22857
                     /gene="mxiD"
                     /locus_tag="OO566_001505"
     CDS             21157..22857
                     /gene="mxiD"
                     /locus_tag="OO566_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6604835.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSRLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22876..23943
                     /gene="mxiC"
                     /locus_tag="OO566_001506"
     CDS             22876..23943
                     /gene="mxiC"
                     /locus_tag="OO566_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6604836.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23956..26016
                     /gene="mxiA"
                     /locus_tag="OO566_001507"
                     /gene_synonym="virH"
     CDS             23956..26016
                     /gene="mxiA"
                     /locus_tag="OO566_001507"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6604837.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26029..26430
                     /gene="spa15"
                     /locus_tag="OO566_001508"
                     /gene_synonym="spaK"
     CDS             26029..26430
                     /gene="spa15"
                     /locus_tag="OO566_001508"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6604838.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26434..27726
                     /gene="spaL"
                     /locus_tag="OO566_001509"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26434..27726
                     /gene="spaL"
                     /locus_tag="OO566_001509"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6604839.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27819..28175
                     /gene="spa13"
                     /locus_tag="OO566_001510"
                     /gene_synonym="spaM"
     CDS             27819..28175
                     /gene="spa13"
                     /locus_tag="OO566_001510"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6604840.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            28144..29022
                     /gene="spa32"
                     /locus_tag="OO566_001511"
     CDS             28144..29022
                     /gene="spa32"
                     /locus_tag="OO566_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6604841.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIAANYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29016..29897
                     /gene="spa33"
                     /locus_tag="OO566_001512"
                     /gene_synonym="spaO"
     CDS             29016..29897
                     /gene="spa33"
                     /locus_tag="OO566_001512"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6604842.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29902..30552
                     /gene="spa24"
                     /locus_tag="OO566_001513"
                     /gene_synonym="spaP"
     CDS             29902..30552
                     /gene="spa24"
                     /locus_tag="OO566_001513"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6604843.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30567..30827
                     /gene="spaQ"
                     /locus_tag="OO566_001514"
     CDS             30567..30827
                     /gene="spaQ"
                     /locus_tag="OO566_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6604844.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30827..31597
                     /gene="spa29"
                     /locus_tag="OO566_001515"
                     /gene_synonym="spaR"
     CDS             30827..31597
                     /gene="spa29"
                     /locus_tag="OO566_001515"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6604845.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31604..32632
                     /gene="spa40"
                     /locus_tag="OO566_001516"
                     /gene_synonym="spaS"
     CDS             31604..32632
                     /gene="spa40"
                     /locus_tag="OO566_001516"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6604846.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSVFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32645..32887
                     /locus_tag="OO566_001517"
     CDS             32645..32887
                     /locus_tag="OO566_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604847.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10463 a         4868 c         6700 g        11464 t
ORIGIN      
        1 tgacacttgt tgactacctg tgcggcatct ggtatcaagg tggcgtgaag ccggttcagg
       61 ttctcatgcg gaacgttgga acctatatcc ccccgatacc aaaggcgaaa tgtaagtgac
      121 aaaatgcaat gaacacgttc tccgttaact gaggagcatg gtgataaata aaggtaaatg
      181 cagaaggtgt agagtctcgc agatttctga catcatagag cctaacatta gatgcataac
      241 gctacagatt gtagaatctc tacaggtgaa atatacactg gttggcgttt atgtcgcaaa
      301 acctgtcatt caggttcatc tcaccaataa ggatatgagt gaagtggagg ataagtgagt
      361 ctgctgtcag agttttctgg tgtatgtcag taactgcgaa ccatgtctgc ttgatatggg
      421 agcttatgga ggctctctgt acaaggttag ggagctaaca aagcagggat aaaaagtccg
      481 cctgatgcag gcacggttcg ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat
      541 gttcaggcta tctttatctt gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca
      601 atagaagaac tgcaggcagt actggtccca catcgtttgc tcagtcaact ggtgaagttc
      661 cgtagcgcgt aaattaatgc tttgcatgaa acgttactgg agtggatgaa agaatacctg
      721 catatatcat tactgcgttg gattaccagt acaacagatt gaatgaactg gacatgttga
      781 tagacggcat tgaaaagcag cttataagca tagtgcggca gagttaattg gagagcactt
      841 cgccttctga gtattaagta agcatggtgg tacctgcgga tactatctat tcactgtgct
      901 cggtcggcag ccttactgac caaggagttg ggggagttga taacaaagtt gaaaaaacag
      961 ctctcaacca aggtggttat tgtaacaata gcaaataagc ttgtgtgacc tgtatcacaa
     1021 taaccgatta tggacgtaaa tacgacaaaa atatgtcagt atcaggcggt attaattgct
     1081 gatgctgatg gacattaact cttaataaaa gggtggatgc tgaaaggttg ttatggtgac
     1141 ggacgtgatg acgacggaaa agagtatgag gttttggatt taaagttcgt tcggggaata
     1201 aggacccggt cagctaattc tcatagtagt tcgcattgct ggatgcaata ctgtgctgca
     1261 attactcgtc gatgtcggaa caacggaagc cttgcaaacg gggatgtatt catacaggtg
     1321 ttatgttata tggggtttta ttgcaggggg ctataccaag agaatatatg atatctcatc
     1381 agactgatgt gagggtaaat gagaatagag taaacgaaca aggttgtttt ttggcgagga
     1441 agcagatgta tgacaattct tgtggagctg ccagtttact ctgtgctgca aaggaattag
     1501 gggttgataa aataccacaa tacaaagggt ctatgtccga aatgacaagg aagagttctc
     1561 ttgatttgga taatcgttgt gagcgggatt tatacttaat aactagtggt aattataatc
     1621 cgagaataca caaagataat atcgcggatg ccggttattc tatgcctgat aagatagtca
     1681 tggctacgag gcttcttgga ttgaatgctt atgtcgttga agaaagtaac attttttcgc
     1741 aagtaattag ttttatttat cccgatgcaa gggacttatt aattggcatg ggatgtaata
     1801 tagtacatca acgagatgtg ttatcttcta accaaagagt tttggaagct gttgctgtat
     1861 cctttattgg agttcctgtc ggcctccact gggtgttgtg tagacctgat ggaagctata
     1921 tggatccagc tgtaggagaa aattattctt gtttttcaac tatggaatta ggtgcaagaa
     1981 gaagtaattc taactttata ggatatacta aaataggtat ttccatagtt ataactaatg
     2041 aggctttgta aattgtcgtt ttttatctgt gaaggagctt gaatattttg gggcgggggg
     2101 ggcataacaa atgatattac tgagtacaaa tttagctata ttgggcggat tagttgtgtg
     2161 ggaagacgaa atgtttctct tgaaataagt gaactccctc aaggaatgat tgtgtgctta
     2221 aatatttaag tgctggtaag tttgagaagg ctgacatatt ttgcagagca aacagtgatt
     2281 gacttggatg atatttttat tttagtcact cgttgtaatt ggagtatgct ttattttatt
     2341 tgaaaataga tgtattttat acccgcaatt taaactgtaa gccctatgag gggtatggat
     2401 attataagtt actctttgac taagcataac ttttggggtt agaaaaataa atctcttgtt
     2461 aataattaat atgattttaa aatcaaactt gtttataatt ataaaataat gtaagtcggt
     2521 cattttatgg gaagattagg agaataaaac agtaattata tttgagaaat ctgctaatat
     2581 attcttaaag cttttttgat actgtgaacg aagtctgtta atggtggatt ttttgtttat
     2641 caccatgtaa aaaaattgag tagtaaaagc gttgactgct aatcctcgct gtttttattg
     2701 aaacgattaa aaatacgctg aatattttcg gccagtcact catcttcaca taaaattttg
     2761 accatcatgc cgcatccctt agttgcacaa ttcattttca atgatatgaa tattactaat
     2821 ttcaccagat aattttctcg tggtcagcag ggtatgagtt ttcaccgtaa ttttaaggaa
     2881 acacagtgcg aggggattga ataacagata gccgacggaa ttaccccacc ggctgaaagt
     2941 caagttcaac gaagattttt attatctgaa ttgggcagtt tacatcagtg ttcgatgttt
     3001 atgaagacga tagatggcga gaaattatat cccgaatagc ttcatctatt tctctctgaa
     3061 ctgttttgtt tattttagat aatgtaaata ttagagttct gtttgtttta ttctcttttc
     3121 tttttatgta agtccgtttg tctttagaaa taaataacga gtcaactttt agcgaagggt
     3181 tttgctttct gtttttgctt ttctttatga tgtttaaaat ttctttttta tatatgtccg
     3241 ggggcaaatt tgtatcaagg tcttttattt cttcctttaa tattggtagt gtagaactaa
     3301 gagattcatt agccttttca aggcctttat aataattgaa taatatcttg tagtcattaa
     3361 agttaagttc tgacgcgatt ggaaatagag atattatttc ctgtggaacg cttgctgcct
     3421 gaaaggcacg agtgactttc gcgcgagaca gattctcttt tttggctatg tctttatagg
     3481 acatccctga tactttcaga aaattaagac caataccaag ttctcggatg ctatgctctt
     3541 ttgctgtttg tatatcgttt gctagttttc tggcatcaag agtagatata tattcttttg
     3601 aatatagaac ttcaagatct gctcctgcat atattgcaga tgctcttcta cgagtgccat
     3661 ccagaatttc aattctacca tcaatctccc ttcctattac agggaagaat tgttgtagct
     3721 ttatagtttt tatgatatcg gctaatgatt cttctgttag ggattcttga tcgcgtacat
     3781 tcgttttttg gttgacgaag gttaaatctt ggatcctttt atgaggaatc ttggctttga
     3841 taaaggaaac tttattacca gaatggagtg taaactcttt cttttggcct tcctttatac
     3901 ttaacaagtc gttgcacaaa tccaccatat cacaccctgt ttattcatat tgatttatat
     3961 tgacaaaagt taaatgcagt gtgagcaaga ttgtatgtaa ctgttgatga tagtcaacgg
     4021 tagtcttaaa aattactatt ttaatagctg gattgatgca atttatcttt attggataaa
     4081 agaataatat tcttgtggag ctctgatgtg agagctccat agatgaaaaa atgcttatca
     4141 attactatat tgattttgta ctatttttga ctacagaaat aagatctcca atagtaataa
     4201 tgtgttctaa tgcagattca tctatacgta gattgaactc aaaactaatc atatccagca
     4261 tttcaatcag agaaagagaa tcggcagaaa gatcttcaat aaatttagtt tcttcagata
     4321 gtttgctgta cgctatacca tagcaaaagg ccactattga taatattttt tcttttatca
     4381 tattttaatc cttattgata ttctttaata cttttgatag ggaagtggtt acatcttttg
     4441 ctgctttata tatattattg ttttcagctg taatatcatt gttatttttt aaatcagata
     4501 tatcatctgt aagtaattct gcagaggtat cgtcaatttg tgacaatacc ttgctgaggg
     4561 cgctagatac ttcttttgcc ttttcataga tcgcatgatt tttatcaatt gtatctgcgt
     4621 tggtttctgt tacgtagttt attttatttg catctgtaac ttttaatttg ttgtttattc
     4681 tgtgttctgt tgtggatttt gtaatgtcct ttgatttttc ctgtaactct cgttcttgtg
     4741 ttcctgattt agttccaatg agagatataa gattacttag ggagtttgtt actttttttg
     4801 aagcttcaaa tatcgtttcc cgtgttaaat atgtgtctcc atttcttgat aaaaaatcag
     4861 tctgaagtac ttttgaatca gtgtttgagt tgaatttatt tatttgcgaa gctactctat
     4921 gagaaagaga agcgctactt ttagttgtat ctgatgagtt atcattactg cttgtcactc
     4981 cttccgggtg gagaggggag gtggttgaag agttctgtat gttcagaggc ctgtgtcccc
     5041 gagaaagaga gttatttgca gatctttctt gactactcgt agttaacagg tctgaaccta
     5101 ttccatccga attatctgga gtatggttga aaaccttttc taaagctgaa ttatttgcaa
     5161 atgtctggac agtatctgat atagagtgtg gtagaacgga agttactttt tgaactagtg
     5221 ctctttgtgg atttagtaat gagtttccgt tacgggataa tagatcgttt gtctgagaag
     5281 taggagactg tgcatcattt tctgggttct cgtgatagct attgtcatat actctgttgt
     5341 caaaaactct attatcgtaa gttctattat cattatttat atgatagtga attacaggtt
     5401 gggatgtttt ctcattggct ccgggggttg gtccgccatc tggcatatct tcgggaggtg
     5461 tagggaggcc atcttggata ggatgcgaat cttttatatc ttctaaaata tcattaagga
     5521 cttttctcaa cagctcagga aacacggcta ctgtatttaa gtctcttgac agtttaccta
     5581 taccaaatcc tttactatca atagtatttt tacgttgatt gcgcaaacta tcaaaggcaa
     5641 gttttgcctg tgcgtcaatt aaatcatcta gtttgagaga tgaaacagtt tcattactta
     5701 actttaattc agcaagaata ttattcttga taaatgatga tacttcagta ttgaggagtt
     5761 tttgtcccaa tgataaagct tcaccagatg tgtgttgttg tgctaatagt gcaagtctgt
     5821 gctcaatgtt ttgtcgtaat tgatggagtt tcttattctt tgccgaccat ggtccaaagg
     5881 cagaatcttg ggataattta tcttttataa ttagttccga atactgtgcg acaatttttt
     5941 gaagtccctt ttcgttttca aaattaagac cattttgatg tactaaggag tacaaaacat
     6001 ttgaaaattc gagaatctcg cttttggcat acgcattcat tgagttggaa taaatgcttc
     6061 ttaacacatc ttctttccct gaggaagaaa ataaaaaatc aagacatttc tgattaaaag
     6121 acgttgcaaa gttttctttt tcagacactg atgctggtgt ttttagagaa gtttgcgaac
     6181 tatggatatc ggatattttg ctttttaact cgctgtattc tgttgatgat ggtgaagttg
     6241 ccttatataa gaatgttggc gcttgagtat tatttacatt atgcatggcg cacctcagaa
     6301 atggagaaaa agtttatctg tatctgtaca tgagcttatt gtactactca aaacctttac
     6361 taaattatca aaaatactat tggcattact gtatttttga actaaagttt gaagattatt
     6421 tttcattgtt tcatcttcgg cagagaatcc ggcattccat gcctgatatt ttgcattatc
     6481 tagcacaacc tcgccatttc cacctagatt atctaagctt tttaacatat tgtctattgg
     6541 ggtcatgttt atactgacaa catatccccc gtttttttga gataccttgc cgattgttcc
     6601 acctaattct gtaagccatt tatttgcttg ttcctgacta acagtattat ttgctggata
     6661 tagcggttta tctttatatt tttccttgag ttcttccaat gcctttttaa gcgagttgac
     6721 ttgtaatttc acggagtttc cgtcgttacc tccgggagag atccagccgg caagactgga
     6781 aagaacagcg ctaaaatctt gatacatttg agtatatgaa ctaacggcat gttcatatac
     6841 tttcagatac tgttcattaa tatcattgat ggagtttgca attttagccc acagttctct
     6901 atgagatatc atttgatctc catcaagctc ggcttctttc ggggctgaat gtaataattc
     6961 tcttgcgtct ttattaattg gatattcgtt cctggaaaga atatccaata gttgagcgga
     7021 tttatttaca tccatgctaa tctgagatga atgtaatgct atttcttcta gcgaagtttt
     7081 agtcaacgtt ttttgtgaaa gctctttctt taatgcctta tttgttgttc tgatattatg
     7141 aagggtgtcg ttaagcatag taagggaact tacaggatga gaactggtcg ttgtttttat
     7201 atcagaatta actgtttcgg ttgatgaacc gttggtattg tttggactga atgatgaggt
     7261 ggaaatacta ttagtcagag ttgttatatt catggttatt tccttacagg aagagccata
     7321 tattattatg gctcttggat atattaagct cgaatgttac cagcaatctg actggctgcc
     7381 gaattccttg attgggtgat gctgtcaatt atattcaata atttttgtat taattgattt
     7441 gtcgcttggg atgcttcttt agatacttgg gatgcttcct ctgcttgttt actgctggcc
     7501 tgactgatta gttgttcttc ttcttcaaga gcagatgcat aacgccctcc tgatgtggat
     7561 atattaccag caacggctga tgtttccatt gttgcacggc caatgttttg tttttgctgc
     7621 gcagaaaggg tattgagctc gtaagttctt ctctgggtgt caattttatc ctgcaaagaa
     7681 atatcgggag aactaagaga agttttatgt tcagttgaca gggatatatt atctggcgcc
     7741 agtttatttt tacctaaaaa ttttgtgcta gagttagttt gtggtgaggt gatatttgta
     7801 tctatttgtt tatttaaccc taatttagaa cctgcaagct ctttttcaag agattgagca
     7861 gtggcaaggt tctttcttaa ggctcctttt tggtcgctaa tccctgaatg cgttttttta
     7921 gcaccgatac ccgttatacc tacttgtgtg actgctccag taatgcttga tgatagggct
     7981 gccaggcctt gccgaacaat gttctctgca gctgattttg tagcatcgaa cgcaatcaat
     8041 gacaattgag agcccaattt attttctgca gtgcggagag cagaaagtag aacggctaca
     8101 ctaataatta aagaaacagc attagaagaa agactggaaa tatccagagt gttctctgga
     8161 gtaagggtgt gactcatctc tgaaagatct ttcatctttt tgtcagtaag gcgagcaatg
     8221 atttctgaat catgctgaac ctgctctgat aactttaaaa gttgatcatc atttaatgtg
     8281 gttgttaata cgggattttt accgacatta agtggaatat gcgctgcaat ctgctgataa
     8341 ttttgtgatt tttgtgtttc ggaagaactt tgagtttgtt ttgtggatat atctgtatat
     8401 aaaatctggg ttggttttgt gttttgaatt tccataacat tctccttatt tgtatcaagc
     8461 agtagtttgt tgcaaaattg cttttgcaac atcagttcta ttagcctgag agttggacat
     8521 tgaggctaat agatctgcaa ttacttcctg caattggccg aatttttcta ttgcttcact
     8581 gatatatttt gacagttgtt caacttgata tttcgataat gtcaggtctg ctagatttgt
     8641 cgacgcgctg ttctggaaaa cagcagaagc gacacttcct cccgcttgtg tggcagagtt
     8701 aacactttct cctaataaaa ctgcttggtt taaatgggtg gaaataattt gtttggatat
     8761 tacttcatca cccgctgcac caagaaattt atttaatctg gcaacagcat tagtgattaa
     8821 atcgtccagt tgagaagaaa aattcttgag aaactttggt ataaggtctg tgagggtttt
     8881 acctattatt ttgccaatat tctctgcaag ttttgctgct gcctgtttac caacagtggc
     8941 tacgagaacg actgctgcaa ctaggacaag agcgcctgcg attgccccca gaatagagcc
     9001 aatcattttg gcttttttcg agtcgacgcc caagccttcg agcatttttg taaatgcatc
     9061 tgaaaggagt ttgattaagg gttcaatgac tgctttcatg atcggattca gggcttgttc
     9121 catgaaggaa ttgccggtcg ctgcttgtac tatagcatcc gtaaccataa gagctaaacc
     9181 aacagctgcc agtgctagag aggctcctcc agaaaaagct gctgcaacaa cactaacgat
     9241 agttaaaagt gccccaagta ttttcccaac acaacccatt actctgttga gttcttctgc
     9301 tttacgtact tcagcagcat actcatcaga ttttctctcc atttcagttt ttcttgattc
     9361 ttggagagac tggaatagag ccagatcatt ttttaaagat tcttcattat tttttccaac
     9421 tagttgaata aaggttgcca tcaattgagt aacactggca agtccggtta atgatttctg
     9481 ctgggttgat agctgttcag cagatgctgt gtttgaaaat gcagaaaaag agtctatttc
     9541 tttttcgagt tgcattgatt tatctgtaag tttgctatga attgacaggg ttttctgctc
     9601 aatcaatgtc ctgtctttaa ctgctgcgtc ttttttgata gtgagttgta tttcttcccg
     9661 gcttaatttt ttcttttctg gtgactctgg gtcgagttcg gataatcttg tttgaatttg
     9721 gttaatttta ttttctaggt cttttatttt agaatctgcg ttttttagtt tattaatttg
     9781 tttttcatag tctctggtta gtccttcagt ttcagataga agagtgttaa ttttatcgga
     9841 gaattctagg tttttttgct gtcttgcctg ttgctgggac ttccaagcag taattttatt
     9901 tgttaatgca gttaaagatt tttcaccgag tatttgaata aggtttccaa ttaaaagcgt
     9961 tagttgggaa cttgcattta atgactttgg tgctttaagt tcagggatta atatatttga
    10021 agttgagtgt gcattagttt tattaatatt ttggttagca gtaagatcag caattgtaag
    10081 agaaaataat ttattagctg catcatttgc agcttggata gtattgtctc caagctcagt
    10141 ggaagccaat attttggcaa gaggaaaacc agtggttgtg gtgcttacat tatgcataat
    10201 aattactcct tgatatcctg aattgcgtcc aagtatgatt gtgcttttat ttttaatttt
    10261 tcatcattgc tgtgttgaat tacgagttcg aagcactctt tagcttttaa gggggctttc
    10321 aaccgaagct ggcattgtcc agtatggaat actggtgtat agtcattttt tcctaatgca
    10381 aaagcgacag cataaaggtc tgctgcttgt tggaactgtt cttttatctg ataaatagct
    10441 gcgagtccca taatgtagtc tacattgtaa aagtcgtata tacataaaaa tctgaagaaa
    10501 acttcagctt cctctattct tcctttgttg taaaagtcat aagcatatga ataaatgtca
    10561 tccatcatat catcaggaat tgcattaata tctttcagtg tagcgccaga gttaattgca
    10621 tcaattactg cagtagagat gctttcattt tcagtgatat ttaaagacat aaggtctcct
    10681 ttttgtctgc tttactttaa taagtataag atttaatata aaagatttaa tttgtattgc
    10741 tttgacggta tacagccttt agaacctctg cgcttatttc tatttgtaaa acttttgttg
    10801 attcattaaa tattttatcg tagacggaac tggatactag atttgtaaga tccttcattt
    10861 gtgtgttaaa caaatccatg tcactgctat agcgttttaa aatctttgct ctgaggccag
    10921 atgaggcaat ctcgtttagt gctgtatgac tgatagatgt ttgtgctgat ttttcatcaa
    10981 gttttatatc cgggaatttt ttttcaatag ctgcaaatat gttttttttg ttatctgagg
    11041 tcaaaacaac tccattgtgt atggccattt catctatact tctgtttatc attgaagaga
    11101 cataagaggt tcgctcttgg ctcatccaac aaaatagttg attccccgaa ttactatctt
    11161 ttattttgtc tatgacgctg tccctgattt tatttatatt caccgggcat ccaaaattct
    11221 tctgaggaaa gcgttgtttt aagttaaaaa cagataatat tttctttaca agcttattgc
    11281 gacttaacga atcaaatgat gggcgaggaa tcgcgaattc aacctgtgga agtattttgt
    11341 ttagaatttg catgataccc cctatatgtt agttcacttc tgaagtgatg tttgcaatgg
    11401 ttagtttaat acaaaataaa aattcataca aagattcaat gagttcatca ggtgaaagat
    11461 attttttgta gtgtttcttg gtcaaaacac attgatgagt atcagggaga agacagatag
    11521 aagaaccata atctctactc aaagagtagt taaggatgag tatgtgttct ggattaataa
    11581 tattttctgt aaaaagttca tgttcattaa gaagtccggt caaaaagatg tattcatcat
    11641 tatctgttat acaaacaggt atttgttctt cgagcaatat aaaggcttga tttttatcgt
    11701 taaaatcaag ctttgcacct gttatttcat aaagtttatc atatagtttg cgacacattt
    11761 ctatggcctt ctatatatta gaatgagagt tattcaataa aagcttattg atctgtaata
    11821 agtcgtgaat gtaaagtttg gttccaagat ctggcgattt aagagaattg taataatcaa
    11881 taatttttat tgatttttcg ataatttctt cttttttatc tgttgcttcg gtttttaatt
    11941 cgtttaatac acgagtcata ttgctgggtg ctgattttac taagtatgag tggcaagcgt
    12001 tgatgaccac atcaaagtct tctgaagata cattttcagt aggatgtttg taaagtgaaa
    12061 ttgataaagg cttgaatgtt tttggtgtgt ggataaggtt aattttattg aaacttttaa
    12121 gatagctgcg tcgtaattga gtcaatctgg aaagatcaaa atcagattca atatacttca
    12181 ttatttcgtt ataagactga ttgagacttt caatgcgttg cctaacttta tttgcatacg
    12241 cgtatacatc gttaggtgta gcaaccaatt taacgtcagg gagtggaaag tatatttcag
    12301 aatttcctgc tttaagaaca ttaagtgcca taccagcaca gttttcagat ttactgatta
    12361 atttataatt tgccaattgt ctgaaagcat ctgctttctt tttgcatata aattttgaca
    12421 tgtttgtttc atcaaggcca aaaaggttat ggctgatttt accatccttt gttttattat
    12481 cacctgataa tggaatgtat actttatcag cactgacccc ccaataacca taggggtctt
    12541 ttttttgatt agctctggga aaaaatattt ttttgttgtt attttgatct tgttttgaat
    12601 ttaataagtt tctaacctct tggcctgcat caagtctttc ggctgtttgg tctgaaatca
    12661 tatttagttt gtcttgctta taggtatcga cactgtaacc taagcgtttt tcaaaaaggt
    12721 agtttatttc tgtatctttt gtaacatccc cgccgggata ccagctgaga taggtatgag
    12781 ttggttcctg tagtaaattg ttttttatac tgatactagt atgccctaaa tgggttatgc
    12841 ctctatttcc ccagcaacga aaaactaaat gtctttcaag atttttggct gtaccgatgc
    12901 catgaaaacg ctctgcggta ttcaacatta atttgatgtc tctacctgtg aaagagtgtt
    12961 gaattgagtt tttagctgct tcataggcgg caaacgcact ttctgcagat ttttcgctag
    13021 ctaatgtatt gaggaaaagc aataaggatt tttgattctc tagtcgataa ttttttagtt
    13081 gttcatttaa tatctgtgaa tttatctttg cattaatagt gcttaatgtt ctgaaaaaga
    13141 gatcctttag gttgaatttc tgtgctatca atataggtcc atgctctgta tcttcaacgc
    13201 gtataggccc ttttgtattg cttgcgtcaa tgaaattgct aattttgagg ctcatacttt
    13261 attaactctc cattacttgg tgattgtggg gatgtaaaat gtatttggca tataatatat
    13321 tcaattaata aatttagaaa cttgagcctg ttaacataat caaattttct tttgctgtac
    13381 ataatatgta cctcgtgagc atatgtagtg ctcgtttcat catgaaatcc cacaagataa
    13441 agtgcctgat gtatcaggct cggagtgtta tagaaaaaga gagaaccctg ttgaataagt
    13501 atcttttttg ttgtaaaacg aggtacaaaa ctgcaatcaa tgccccattt atgactgagg
    13561 ataattaaat gcacataact aatttgggat tgcatcaggt ttcatttcaa agcggagatt
    13621 cctataaagg cgcagaagaa accggaaagc acaaaggtgt aagcgtgatt tcatatcaaa
    13681 gagttaaaaa tggagaaaga aataagggga tcgaagcgct taatcgatta tatttacaga
    13741 accaaacaag cttaacagga aaaagccttt tgtttgcgcg tgacagagct gaagtttttt
    13801 acgaggcaat aaagttggca ggtggtgata cgtcaaaaat taaagccatg atggaacgat
    13861 tagataccta taaacttggt gaagttaata aaagacatat taatgagctt aataaagtaa
    13921 taagtgaaga gataagagca cagctaggca ttaaaaataa aaaggaattg caaacgaaaa
    13981 taaaacaaat atttactgat tatttaaata ataagaattg gggaccggtc gacaagaata
    14041 ttagtcatca tgggaaaaat tatggttttc aattaacacc tgcctctcat atgaagatag
    14101 ggaataaaaa tatatttgtc aaagagtata atggaaaagg aatttgttgt gcttctacga
    14161 gagagagtga gcatatcgcg aatatgtggc tttcaaaagt ggtggatgac gaggggaagg
    14221 aaattttttc gggtatcaga catggagtta tatcagccta tggattgaaa aaaaactcat
    14281 ctgaaagagc tgttgctgct cgtaataaag ctgaggagtt agtaagcgca gcattatata
    14341 gcagaccaga gttattatca caggctttgt ctggtaaaac agtagattta aagattgttt
    14401 cgacttctct tctgacgccg accagtttaa ccgggggaga ggagagtatg ttaaaagatc
    14461 aggtgaacgc attaaaaggg ttaaactcta aaagggggaa gccaacgaaa ttattaattc
    14521 ggaatagtga tggtcttctg aaagaagtaa gcgttaatct aaaagtggtg acatttaact
    14581 ttggtgtaaa tgaattagcg ctaaaaatgg gcttaggctg gaggaatgtt gacaagctta
    14641 atgatgaatc aatatgttct ttgttggggg ataatttcct caaaaatggt gtgattggag
    14701 gttgggctgc agaagcaata gaaaaaaatc caccatgtaa gaatgatgtg atatatctgg
    14761 ctaaccagat aaaagagatt ataaacaaga agttacaaaa aaatgataat ggagagccgt
    14821 ataaattgcc acaaagaatg actctattgg cttatactat aggtgctgta ccttgctgga
    14881 attgtaagag tgggaaggac agaacaggta tgcaggatgc tgaaattaaa agagaaataa
    14941 taagaaaaca tgaaacaggt cagttttctc aattaaatag taaattatcc tcagaagaga
    15001 aaagattatt ttctactatt ctaatgaata gtggtaatat ggaaatccaa gagatgaata
    15061 ctggtgttcc cggaaataaa gtcatgaaaa aattgccgct atcttcgtta gagctatctt
    15121 attctgaaag aataggggac ccaaaaatat ggaatatggt gaaagggtat tcgtcatttg
    15181 tataagagga atatatggaa gatttagcag atgttatttg ccgagccttg ggtatccctt
    15241 taattgatat cgatgatcaa gcaatcatgc ttgatgatga tgtgctaata tatattgaaa
    15301 aagaaggaga ttcaataaat ttattatgcc ctttttgtgc attgcctgaa aatataaatg
    15361 atcttatata tgcgttgagc ctaaattact cagaaaagat atgcttagct actgatgatg
    15421 aaggcggaaa tctaattgcg cgattagatc ttaccgggat aaatgaattc gaggatgtat
    15481 atgttaatac agaatattat atttcgcgtg tgcgttggct gaaggatgaa tttgcgcgaa
    15541 gaatgaaggg gtattaatgt cccgtttcgt atttattttg ctgtgtttta tcccatattt
    15601 agggagagct gattgttggg ataaggctgg tgaaaggtac aatattccat caagtttgtt
    15661 aaaagcgatt gcggaaaaag aatccggatt taataaatct gcagtcaatg ttaataacaa
    15721 tggaagtaaa gattatggta taatgcaaat aaatgacttt cattctaaaa gacttagaga
    15781 aatgggatat tctgaggaaa tgctaattag tcatccatgt ctttctgtac attatgcagc
    15841 aaagttatta aatgagttta tgatgatgta tggaagaggg tgggaggctg ttggagccta
    15901 taatgcagga acgtctccga aaaagaaaaa agaaagatta aagtacgcag aggatatcta
    15961 tagacgctat ttgcggatag cagcagagag caagcagaat aatcgaagga tataagagga
    16021 tttatgtctg aggcaaagaa ctcaaatctt gcaccattca ggttattagt gaaactcaca
    16081 aacggtgtcg gggatgagtt tccgttatat tatgggaata acttaattgt tctcggacgg
    16141 acgatagaaa cacttgagtt tggaaacgac aatttcccag aaaatattat tcctgtaaca
    16201 gatagtaaat cggatggtat aatttatttg accataagca aagataatat ctgtcaattt
    16261 agtgatgaaa aaggtgagca aattgatatc aattcacaat ttaattcatt tgaatatgac
    16321 gggatttctt ttcaccttaa aaatatgagg gaggataaat cgagaggcca tatactaaac
    16381 ggaatgtata agaaccactc ggtgtttttt ttctttgctg ttattgttgt gttaattata
    16441 attttttccc tctctttaaa gaaagatgaa gtaaaagaaa ttgctgaaat tatagatgat
    16501 aagagatatg gaatagtcaa cactggacag tgcaattata ttcttgccga aacacaaaat
    16561 gatgcggtat gggcgtctgt agccttaaat aaaaccggat ttaccaaatg cagatatatt
    16621 ctggtgtcaa acaaagaaat aaatagaatt caacaatata tcaatcaacg attcccattt
    16681 atcaatttgt atgtgttgaa tttagtgagt gataaagcgg aattactggt gtttttaagt
    16741 aaagaaagaa atagttcaaa agataccgaa cttgataaat tgaaaaatgc tctgattgtt
    16801 gaatttccgt atatcaaaaa tattaaattc aactatctta gcgatcataa tgcaaggggg
    16861 gatgctaaag gtattttcac aaaggtaaat gtccagtata aagagatttg tgaaaataat
    16921 aaagttacat attctgtcag agaagaactg acagatgaaa agcttgagct cattaataga
    16981 ctgatttctg aacataaaaa aatatatgga gatcagtata ttgagttttc tgtacttttg
    17041 atagatgatg attttaaagg taaatcatat cttaacagca aagacagtta tgtaatgttg
    17101 aatgataaac actggttttt tttagataaa aataagtgag gataaaatga gtgttacagt
    17161 accgaatgat gattggacat tgagttcatt atctgaaact tttgatgatg gaactcaaac
    17221 attacaaggt gaactaacat tggcactaga taaattagct agaaatcctt cgaatccaca
    17281 gttgctggct gaataccaaa gtaaattatc tgaatataca ttatatagga acgcgcaatc
    17341 caatacagtg aaagtgatta aggatgttga tgctgcaatt attcaaaact tcagataata
    17401 gggagcattc atgaattaca tttatccagt caatcaggtt gatattatca aagccagtga
    17461 ttttcaatct caagagatat caagtctgga agacgtcgtg tcggctaaat atagtgatat
    17521 taagatggat acagatattc aagtatcaca aataatggag atggtaagca atccagaatc
    17581 attaaaccca gaatctttgg ccaagttaca gacgacgctc tcaaattatt caataggagt
    17641 atcattagct ggcacgttag caagaaaaac agtttcggct gttgaaactt tattaaagtc
    17701 ttaatttata tgattaggta taaaggtttt attttattct tgttgctgat gttgattgga
    17761 tgtgagcaac gtgaagagtt aatttctaat ttatctcaaa gacaggcaaa tgaaataata
    17821 tctgtgctag aacgccataa tattactgct agaaaagttg atggaggtaa acaggggatc
    17881 tcggtacaag tcgaaaaggg gacatttgca tcggcagttg atttgatgcg catgtacgat
    17941 ttgccaaatc cggagagagt tgatatctca caaatgtttc ctacagattc attagtgtct
    18001 tctccaagag ctgaaaaggc ccgtttatat agtgctattg agcaacggct ggaacagtct
    18061 ttagtttcta ttggtggtgt tatttcggca aaaatacatg ttagctatga tcttgaagaa
    18121 aaaaatatat cttcaaaacc gatgcatata tcagtaatcg ctatatatga ctcaccgaaa
    18181 gagtctgaac tattagttag taatattaag cgatttttga aaaacacctt ttctgatgtt
    18241 aagtatgaaa atatatctgt catattaact ccgaaagaag aatatgttta tacaaatgta
    18301 caacctgtta aggaagttaa atcggaattt ttaacaaatg aagtaatata tttatttctc
    18361 gggatggctg tactagttgt cattcttttg gtatgggcat tcaaaacagg gtggttcaag
    18421 agaaacaaaa tatgataaga atggatggaa tttataaaaa atatctttca ataatttttg
    18481 atccagcgtt ctatataaat agaaatcggt tgaatttgcc ttctgaactg ttagaaaatg
    18541 gcgtaatcag aagtgagatt aataatctca taattaataa atatgatcta aattgcgata
    18601 ttgaaccttt aagcggggta accgctatgt ttgttgccaa ctggaattta cttccagctg
    18661 ttgcttattt tatcggtagt caagagtctc ggttaattaa tcatagtgag atggttatta
    18721 gttattatgg gggcaaaatc agtaagcaag gggaggccgc tattcgaagt ggtttttggc
    18781 atcttattgc gtggaaggaa aatatatctg ttggtatata tgaaagaata aacttattat
    18841 ttaatccaat tgctttggag ggaaattata cgcctgtaga aagaaatttg tcccgattaa
    18901 atgaaggtat gcaatatgca aaaaggcact ttaccggtat ccagacatca tgcctatgat
    18961 ggtgttgtta taaagagaat tgaaaaggaa ttatgtaaaa ccataaaaga tagagacact
    19021 gagagtaaga ggaaagcaat ttgtgttata aaggaggcta cgaaaaaagc tgaatcactg
    19081 cgtatagatg ctgtatgtga tggttatcaa attggtatac agactgcttt tgagcatatt
    19141 attgactata tttgtgaatg gaagttaaag caaaatgaaa acagaagaaa tattgaggac
    19201 tatataacta gtttgctttc agaaaattta catgatgaga ggatcattag cactttattg
    19261 gagcagtggc ttagttcgtt gcgtaataca gtaacagaat taaaagtggt tttgcccaag
    19321 tgtaatttag cactgagtaa aaagttagaa cttgatctcc ataaatatcg ctcagatgtc
    19381 aagattattt taaaatattc tgaagggaat aattatatat tctgtagtgg taatcaagta
    19441 gtggagttta gtcctcagga tgtaatatca ggtgttaaaa tagaacttgc ggaaaaatta
    19501 acaaaaaatg ataaaaaata tttcaaagag ttagcacata agaaactgag acaaattgca
    19561 gaagacttgt taaaagagaa tcctgttaat gattaatcaa ataaatgcaa gcaatgctct
    19621 gcagcaaaga ttaaatagtg aagaatttgt taatttaaat gagagattat catcttcgca
    19681 atcatttgat gaggatataa tctatgaaat aatgcaatat ttctcacaga gtgaattaaa
    19741 ttccatagat aatgatgaac tgcacaataa gattgaacaa ttattcaatt cacgttttcc
    19801 ttatcttaca gctgcacaaa aaagttcact tcttaataaa ctcatagatg caaatcaata
    19861 tgtcgatttg catgagggat tttatgcatc attgtccata tataacaata ttgatttcta
    19921 tattaagact acaaccttcg attcattaat tagtgtcttt gaagcaggga gagaggcaga
    19981 tgattcgaca tggtagtaat aagttgaaaa tatttatttt aagtatattg ctattaacac
    20041 tgagtgggtg tgctttaaag tcatcatcta attctgaaaa agaatggcat attgttcctg
    20101 taagtaagga ttatttttct attccaaatg atttattatg gtcgtttaat acaaccaata
    20161 aaagtataaa tgtttactct aaatgtatta gtggtaaggc ggtttatagt tttaatgcag
    20221 gtaaattcat gggcaacttt aatgttaagg aagtagatgg gtgcttcatg gatgcacaaa
    20281 agatagctat agataaacta ttttctatgc tgaaagacgg ggttgtttta aaaggtaata
    20341 agataaatga taccatcctt atagagaagg atggggaagt taaattaaaa ttaattcgag
    20401 ggatataatt gtattgtgag taaatataaa ggtctaaata caagtaatat gttttacatt
    20461 tactctagtg gacatgaacc agttaacgtt gagcttgtaa aagataaaga acgtaacata
    20521 attgagctgg ctccagcatg gaagggtttt tttttgtccg aaatcaaaat ataaaattca
    20581 gtgataatgt gaactatcat tatcgcttta acattaatag ttgtgcaaaa tttcttgctt
    20641 tttgggacta tttcagtggt gctctagtag agcatagcca tgctgaaaaa tgtattcatt
    20701 tctatcatga gaatgatctt cgggatagtt gtaataccga atcgatgctt gataagttaa
    20761 tgttaagatt catcttttca tctgaccaga atgtaagtaa tgcactggct atgatacgaa
    20821 tgactgagtc atatcatttg gttctttatc ttctgagaac tattgagaaa gaaaaggagg
    20881 taagaataaa gagtctgaca gagcattatg gtgtgtcgga ggcttatttt agatctttat
    20941 gtagaaaagc tttaggggca aaagtcaaag aacaactgaa cacttggcgg ttagtaaatg
    21001 gtttattaga tgtatttttg cacaatcaaa caatcacttc tgctgccatg aataatgggt
    21061 atgcatcaac atcccatttt tcaaatgaga taaaaacacg actaggcttt tctgcaagag
    21121 aattatcgaa tattactttt ttagtgaaaa aaataaatga aaaaatttaa tattaaatct
    21181 ttgactctct tgattgtatt gttaccattg atagttaatg ctaataatat tgactctcgt
    21241 ttattagaac aaaatgacat agcgaaatat gttgctcaaa gtgatacggt aggatctttt
    21301 tttgaacgtt tttctgcgtt attaaattat cctattgttg ttagcaaaca agctgcaaaa
    21361 aagagaatat ctggggagtt tgatttatct aatccagaag aaatgcttga aaaactgaca
    21421 ttacttgtgg gactcatatg gtataaagat ggaaatgcat tatacatata tgactctgga
    21481 gaattaataa gcaaagttat tttgctggag aatatatcac ttaattatct tattcagtat
    21541 ctgaaggatg ctaaccttta tgatcacaga tatcctataa gaggaaacat tagtgataaa
    21601 acattctata tatctggtcc tccggcattg gttgaactgg tagctaatac agcaactttg
    21661 cttgataagc aggtttcttc tattgggaca gataaagtta attttggggt tataaaatta
    21721 aaaaatacat ttgttagtga cagaacttat aatatgagag gggaggatat tgtcatccca
    21781 ggggtggcta ctgttgtaga acgtttatta aataatggta aggctctgag taacagacaa
    21841 gcccaaaatg atccgatgcc accctttaat attacacaga aagtatctga ggatagtaat
    21901 gacttttcat ttagttcagt taccaatagt tctatattag aggatgtatc acttattgca
    21961 tatccagaaa ccaattctat attagtcaaa ggtaatgatc agcaaataca gattattcgc
    22021 gatattatca ctcagttaga tgtagccaaa cgacatatag aattatcatt atggataatc
    22081 gatattgata aatctgaatt aaataacctt ggtgttaatt ggcaaggaac ggcttcattt
    22141 ggtgattcgt ttggtgcgtc atttaatatg agctcatcag catcgataag cactttggac
    22201 ggcaataagt ttattgcttc agttatggca ttaaatcaga aaaagaaagc aaatgtagtt
    22261 tcaaggccgg tgattttgac acaagagaat attccggcta tatttgataa taacaggact
    22321 ttttatgtgt cattagttgg tgagcgcaat tcttcgttgg agcatgttac gtatggaaca
    22381 ttgataaatg ttatacctcg cttttcatct cggggacaaa ttgagatgtc tttaacgata
    22441 gaggatggaa caggaaatag tcagtcaaat tacaattata ataatgaaaa tacatctgtc
    22501 cttccagaag ttggaaggac caaaatcagt acaatagcga gagttccaca aggcaaaagt
    22561 ttgttgatag gtggttatac gcatgaaacg aactctaacg aaatagtatc tattcctttt
    22621 ctctcctcta ttcctgtaat cggtaatgtg ttcaagtata aaacaagtaa tatcagtaat
    22681 attgtaaggg tttttctgat tcaaccccgt gagataaagg agtcaagtta ttataatact
    22741 gcagaatata aaagcctcat atctgaaaga gaaatacaaa agactacaca aataatccca
    22801 tctgaaacta ctttgctgga agacgaaaaa tcattggttt catacttaaa ttactaacta
    22861 taaagtaggt gatgtatgct tgatgttaaa aatacaggag tttttagctc tgcattcatt
    22921 gataagttga atgcaatgac aaattcagat gatggagatg agactgctga tgcagagctt
    22981 gattctggct tggctaatag caagtatatt gactcatctg atgagatggc ttccgctctt
    23041 tcgtcattta taaacagaag agaccttgag aaactgaaag gaacaaatag tgatagtcag
    23101 gaacgtattt tagatgggga agaagatgaa attaatcaca agatttttga tttaaagaga
    23161 acgttaaaag ataaccttcc tctagatcgg gattttatag acagactaaa gagatatttt
    23221 aaagatccaa gtgatcaagt cttagcatta agggaacttt tgaatgaaaa agatcttact
    23281 gctgaacaag tcgaattatt aactaaaatt attaatgaga taatatcagg tagtgaaaaa
    23341 agtgttaatg ctggaataaa ttcagctata caggctaaat tatttggcaa caaaatgaaa
    23401 cttgaaccac agcttttgcg tgcatgttat cgtggtttta tcatggggaa catatcaaca
    23461 acagatcagt atatagaatg gcttggtaat tttggtttta atcacagaca tacaattgtg
    23521 aattttgtag agcagtcact gattgtagac atggattctg agaaaccgag ctgtaatgct
    23581 tatgagtttg gttttgtgtt atctaaatta attgcaatta agatgattag aacttcagac
    23641 gtaattttta tgaagaaact ggaatcctca agcttgctaa aagatggcag tttaagtgca
    23701 gagcagctat tgctaacttt attatatatt tttcaatatc caagtgaaag tgagcaaatt
    23761 cttacttctg ttatagaagt atcacaagcc agtcatgagg attctgtagt gtatcaaaca
    23821 tatctatctt ctgttaatga aagtcctcat gatatattta aaagtgaaag tgaaagagaa
    23881 attgcgatca atattctacg agagcttgtc acaagtgcat acaagaaaga gctttctaga
    23941 taacaggaga taaaagtgat ccagtctttt cttaagcaag taagtactaa gcctgaatta
    24001 attatattag tgcttatggt tatgatcatt gctatgttga tcataccatt acctacatac
    24061 cttgttgatt ttttaatagg gcttaatatt gtacttgcta ttctcgtttt tatgggatca
    24121 ttttatattg aaagaatatt gagtttttct acattccctt cggttttgtt gataacgaca
    24181 ttatttcgtc tcgctttatc aataagtact agtcgactaa ttttagttga tgcggatgcg
    24241 ggtaaaatta ttactacatt tggccagttt gtcattggtg atagtctggc tgtaggtttt
    24301 gttatttttt caatagtaac tgttgtgcag ttcattgtta taacaaaagg atctgaaagg
    24361 gttgcggagg tagctgctcg cttttctctt gacggtatgc cggggaaaca aatgagcatt
    24421 gatgcggatt taaaggctgg aattattgat gccgcaggag ctaaagaaag acgcagtatt
    24481 ctggaacgtg agagtcaatt gtatggttca tttgatggag caatgaagtt cattaaaggt
    24541 gatgcaattg ctggcatcat tattattttt gtcaatttaa taggaggtat ttctgttggt
    24601 atgagtcaac atggaatgtc cctatctggt gcattatcta cttataccat actaactatt
    24661 ggtgacggat tggtctctca gattcctgct ctgttaattt ctataagtgc aggatttatc
    24721 gttacaagag ttaatggtga tagcgataat atgggacgta acataatgtc ccagatattt
    24781 gggaatcctt ttgttctcat tgttacatca gctcttgcct tggcgatagg gatgttgcca
    24841 ggttttccgt tttttgtttt tttcctgata gcagttactt tgacggcttt attctattat
    24901 aaaaaggtcg tagaaaaaga aaaaagtctg tctgagtctg attctagtgg ctatactggt
    24961 acatttgata ttgataatag ccatgactca tctttggcaa tgatagaaaa tctggatgct
    25021 attagttcag aaaccgttcc tttaatttta ttatttgccg aaaataagat aaatgcaaat
    25081 gatatggaag gtcttattga aaggataaga agtcagttct ttattgatta tggtgtcagg
    25141 cttccaacta ttttatatag aacaagcaat gagcttaagg tcgatgatat tgttttgtta
    25201 ataaatgagg tgcgtgccga tagttttaat atatattttg acaaagtgtg tattacagat
    25261 gaaaatggag atatagatgc tctaggtatt cctgttgttt caacttcata taatgaacgt
    25321 gttatttctt gggtagacgt ttcatataca gaaaatctaa ctaatattga tgctaaaatt
    25381 aaaagtgctc aagatgaatt ttatcaccag ttgtcacaag ctttattaaa caacataaat
    25441 gagatttttg gtatacaaga aacaaaaaat atgttagatc agtttgaaaa tcggtatcct
    25501 gatctattaa aggaagtctt ccgacatgtg actatacaga gaatttctga ggtattacaa
    25561 agattgcttg gagaaaatat ttctgttcgc aatttaaaac ttattatgga gtctttggcg
    25621 ctttgggctc ccagagaaaa agatgtcata acattagttg aacatgtccg tgcatcactt
    25681 tctaggtata tttgtagtaa aatagctgtt tctggtgaga ttaaagttgt gatgctttcc
    25741 ggatatattg aggatgcaat aagaaaaggg ataaggcaaa cctctggtgg ctctttcttg
    25801 aatatggata tagaggtttc ggatgaggta atggaaactt tagcacatgc tttgagagaa
    25861 ttgagaaatg caaaaaaaaa tttcgttctt ttggtatcag tagatatacg taggtttgtt
    25921 aaaagactta tagataacag atttaagagt atactcgtta tatcgtatgc tgagattgat
    25981 gaagcatata ccattaatgt attaaagact atttagtgag gtttaaatat gagtaacatt
    26041 aatttagttc aattagttag agatagtctt ttcacgattg gttgtcctcc atcaattatt
    26101 acagatttag acagccattc agcgattacc atttctttgg atagtatgcc tgctattaat
    26161 atagctcttg tcaatgagca ggttatgtta tgggctaatt ttgatgcacc aagtgatgta
    26221 aaacttcagt catctgctta taacatatta aatttaatgt taatgaactt tagttatagc
    26281 attaatgagc tggtagagct tcatcgttct gatgagtact tacagttaag agttgttatt
    26341 aaagatgact acgttcacga tgggattgtt tttgcagaga ttctgcatga gttttatcag
    26401 aggatggaaa tcttaaatga ggtcttataa tcaatgagct atacaaaatt gctcactcaa
    26461 ttatcttttc ctaatagaat ctcggggcca atcttggaaa caagtcttag cgatgtttcg
    26521 attggtgaga tttgtaacat tcaggctgga attgaaagta atgaaattgt tgcaagagct
    26581 caggttgtag gatttcatga tgaaaaaaca atattaagct tgattggaaa ttctcgtgga
    26641 ctttcacggc aaacgttgat taagcccact gcccagtttc ttcatacgca agtcggccgt
    26701 ggattattgg gagcagtagt caatccttta ggggaggtta ctgataagtt tgctgttacg
    26761 gataacagtg aaattcttta tcgacctgta gataatgctc ctccgctata tagtgaaagg
    26821 gctgcaattg agaagccttt tttaacaggt attaaggtta ttgattcttt actcacgtgt
    26881 ggtgaaggac agcgaatggg gatttttgcg tcagctggtt gtggcaaaac ttttctcatg
    26941 aatatgctca ttgaacatag tggtgctgat atatatgtta ttgggttaat tggtgagcga
    27001 ggtcgagagg ttacagaaac ggttgattat ttgaaaaact ctgagaaaaa aagcaggtgt
    27061 gttttagtat atgcaacttc ggattactct tcggttgatc gttgtaatgc tgcatatata
    27121 gccactgcta tagccgaatt ttttaggact gaaggacata aagtagcgct ttttattgat
    27181 tcattaacaa ggtatgccag agcattacgt gatgtggcct tagccgctgg agaatcacct
    27241 gccagaagag gctatccggt ttcggttttt gatagcttac ccagacttct tgaaaggcca
    27301 ggaaagttaa aggcaggtgg ctctattact gcattttaca ctgttctttt ggaggatgat
    27361 gattttgctg atccattagc tgaagaggta agatccattt tagatggaca tatatatttg
    27421 agcagaaatc tagcccaaaa aggacaattt cctgcaattg attccttaaa aagtataagc
    27481 agggtattta cacaggttgt tgatgaaaaa catcgtatta tggccgctgc atttcgggag
    27541 ctactttctg aaatagaaga gctaaggaca attattgact ttggtgaata caaaccgggg
    27601 gagaatgcct ctcaggataa aatctacaac aaaatatctg ttgttgagag ttttctgaag
    27661 caagattatc gactcggttt tacttatgag cagacaatgg agcttattgg tgaaacaatt
    27721 agataaggtt ttaaaaatca aagataaata tcaacggtct gttaaattaa ttgaagcaca
    27781 catattaaca ctaaaaaaaa aactctttgt atcgagatgt ggaggcatta gataaaagga
    27841 ttatatactt cctgcaactg gagaatgact tagaacctgt aggcgctcag agcgttagtc
    27901 agcttttcaa tacaagacgt aagatcgcta tagtaaaaaa gcatattatc caatatcagt
    27961 ctgaacgaat tcttctaaag ggaagaattg aagaaattca aaaagatatt gatgaggcaa
    28021 atgcttctaa aagaaaactg cttcataaag agagtaaaat atgtaaacgg attggcttga
    28081 ttaaaagaaa taactttgcc aaacagttga tacttgatga gttatcacaa gaggatatga
    28141 agtatggcat tagacaatat aaacctaaat tttagtagtg acaaacagat agaaaaatgt
    28201 gagaaactat cttcaataga taatattgat agtctcgttt tgaagaagaa gaggaaggtt
    28261 gaaattcctg agtactcttt aattgcagct aactatttta ctattgataa gcactttgaa
    28321 cataagcatg ataaaggaga aatttatagt ggcattaaaa atgcgttcga acttagaaac
    28381 gaacgagcga catattctga tattccagaa tcaatggcca ttaaagaaaa tattttgata
    28441 ccagatcaag atatcaaagc aagggaaaaa ataaatatcg gcgatatgag ggggatcttt
    28501 tcatataata agagtggaaa tgcagacaag aacttcgaaa gaagtcatac ttcttctgta
    28561 aaccctgata atctgctaga atctgataat agaaatggtc aaattggttt aaaaaatcat
    28621 agcttgtcta ttgataagaa tattgctgac atcatttctt tactaaatgg aagtgttgct
    28681 aaatcatttg agctccctgt aatgaataaa aatactgcag acataacccc atccatgtca
    28741 ttgcaagaaa aatcaatagt tgaaaatgat aaaaatgttt ttcaaaaaaa tagtgaaatg
    28801 acttaccact ttaaacagtg gggggctgga cattctgtta gtatttcagt ggagtctggt
    28861 tcttttgttc taaaaccgtc agatcaattt gtaggaaata aacttgactt aattttgaaa
    28921 caagatgctg agggtaatta cagatttgat agcagtcaac ataataaggg gaataaaaat
    28981 aatagtacag gatataatga acagagtgaa gaagaatgct aagaattaaa cattttgacg
    29041 ctaacgaaaa actacagatt ttatatgcaa agcaactctg cgagcgtttt tcgattcaga
    29101 cattcaaaaa taaatttaca ggcagtgaaa gtttagtcac tcttacttcc gtgtgtgggg
    29161 attgggtaat tcgtattgat acattatctt ttttgaaaaa aaaatacgag gtattttcag
    29221 gattttctac acaagaatct ttactgcatt tatcaaaatg tgtctttata gagtcgtcat
    29281 ctgtattttc gattccagaa ctgtctgata agattacttt ccggatcacg aatgaaatcc
    29341 agtatgcaac tactgggagt catttatgct gtttttcctc ttctttaggt attatttatt
    29401 ttgacaagat gccggtatta cgtaatcaag tttctcttga cttattgcat catcttttag
    29461 agttttgctt aggttcatct aatgtaaggc tggctacttt aaaaagaatt cgcactggtg
    29521 atataatcat agttcagaaa ctttataatt tattattgtg taatcaagtt attattgggg
    29581 attatattgt gaatgataat aatgaggcaa aaattaatct gtcagaaagt aatggtgagt
    29641 cagaacacac agaagtttct ttggcattat tcaattatga tgatatcaat gtaaaagtgg
    29701 actttattct tttagaaaaa aatatgacaa tcaatgaact aaaaatgtat gtagaaaacg
    29761 aattattcaa gtttcccgat gacatagtta aacatgtaaa tattaaagta aatggttctt
    29821 tggttgggca tggggaactt gtttctattg aggatggtta tggtatcgag attagttctt
    29881 ggatggtaaa ggagtaatct catgctgagt gacatgtccc tcatagctac actttctttt
    29941 tttacgctat taccattttt agttgctgct ggtacctgtt atataaagtt ttctattgtt
    30001 tttgtaatgg tacgaaatgc acttggtttg cagcaggttc catcaaatat gactctcaat
    30061 ggtattgcat tgattatggc tttgttcgtg atgaaaccca ttattgaagc tggttatgaa
    30121 aattatctaa atggccctca aaaatttgat acaatatctg atattgtccg tttttctgat
    30181 tctggcttga tggaatataa gcaatacttg aaaaagcata cagatcttga gttagcacgt
    30241 tttttccaaa ggtctgaaga agaaaatgct gatttaaaga gtgcagagaa taatgattat
    30301 tcattgtttt ccctcttgcc tgcatatgcg ctaagtgaaa tcaaagatgc gttcaaaata
    30361 ggtttttatt tatatttgcc atttgttgtt gttgatcttg ttatttcaag cattttactt
    30421 gcacttggta tgatgatgat gagtccaatc acaatatcag taccaataaa acttgtatta
    30481 tttgttgcgc ttgatgggtg gggtatcctg tctaaggcct tgattgaaca atatatcaat
    30541 attcctgctt agatgatgga ggtaatgtgt ctgatatagt ttatatgggt aataaggctc
    30601 tttatttaat ccttatcttt tccttatggc cagtaggtat agctacggtt attggattat
    30661 ctattggttt gttacagaca gtgactcaac tccaagagca gacacttcct tttggtataa
    30721 agcttatagg tgtctcaata tctttgctac ttctttctgg atggtatggt gaggttttat
    30781 tgtctttttg tcatgaaata atgtttttaa ttaagagtgg ggtttgatgg acatttcaag
    30841 ctggttcgaa agtattcatg tgtttttaat actcctgaac ggcgtttttt ttagattggc
    30901 tccattattt ttctttcttc catttttaaa taacggtata atttctccat ctattagaat
    30961 acctgtgatt tttcttgttg catcaggatt aattacttct ggtaaggtag acataggttc
    31021 ttctgttttt gaacatgttt atttccttat gttcaaggaa ataattgttg gcctccttct
    31081 ctctttttgc ttgtctcttc ccttttggat atttcatgct gttggtagca ttattgacaa
    31141 ccagcgtggg gcaacgctta gtagttcaat tgatcctgcc aatggtgttg atacgtctga
    31201 gttggcaaaa tttttcaatc ttttttctgc agttgtattt ctatacagtg gtggtatggt
    31261 ctttatttta gaatccatac aattgtctta taatatatgc ccgttatttt ctcaatgttc
    31321 tttccgtgtc tcaaatatct taacatttct gactttattg gcaagtcagg ctgttatttt
    31381 agccagtcct gttatgatag tattgttact atcagaagta ttacttggtg tattatcgag
    31441 atttgctccg cagatgaatg ctttttccgt atcattaact attaaaagtt tacttgcaat
    31501 atttattatt ttcatctgtt cttctactat ttacttttct aaagttcaat ttttcctcgg
    31561 tgaacataaa tttttcacaa atctatttgt tagataaaat attatggcaa ataaaacaga
    31621 aaagccgaca cctaaaaaac taaaggatgc cgcaaaaaaa ggacagtcat ttaaatttaa
    31681 ggatttaacg actgttgtta ttattctggt agggacattt actataatat cattcttttc
    31741 cttaagtgat gtaatgctct tatacagata tgtaataatt aatgacttcg aaattaatga
    31801 gggtaaatac ttttttgcag tggttattgt cttttttaag ataattggct tcccactttt
    31861 tttctgtgtt ctttcggctg tgttgccaac attggttcaa acaaagtttg ttcttgcgac
    31921 taaagctatc aagattgatt tttcagtatt aaaccctgtt aaagggttaa aaaaaatatt
    31981 tagtataaag acaataaaag aatttttcaa aagcattctg cttcttatta ttctagcatt
    32041 aacaacctat ttcttttgga ttaatgaccg aaaaataatt ttttctcagg tgttttctag
    32101 tgttgatggc ttatatctta tttgggggag gctgtttaag gatataatat tatttttctt
    32161 ggcattttct gtttttgtta ttattcttga ctttgtgatt gagttcattt tatacatgaa
    32221 agatatgatg atggataaac aggagataaa aagagaatat atagagcaag agggacactt
    32281 tgagacaaag tcgagaaggc gtgagttgca tatcgagatt ctttcagagc agactaaatc
    32341 tgatatacgt aattcaaaat tagtggtaat gaacccgact catattgcaa ttggtattta
    32401 ttttaatcca gaaatagcgc ctgcaccttt tatttctctc attgaaacta accagtgtgc
    32461 cttggctgtc agaaaatatg caaatgaagt tggtataccg actgtgcgtg atgtgaaatt
    32521 agctagaaaa ctatataaaa cacatacaaa atatagtttt gttgattttg aacacttgga
    32581 tgaagtccta cgtcttattg tttggcttga gcaggttgaa aacactcatt aataagtaaa
    32641 ggagatgtgt tatatgggag ttaatttctg taataaaata ggtattgatc agtctgaatt
    32701 tgaaatagaa tcttccatca ttaactccat tgctaatgag gtattgaacc caatatcttt
    32761 ccttagcaat aaggatataa taaatgtttt actcagaaag atttcatctg aatgtgacct
    32821 ggttagaaaa gacatttatc gttgtgctct ggagttggtc gttgaaaaaa cacctgatga
    32881 tttataatca ttcataataa ctcttttgct gatataaata tttttgttga agatatttat
    32941 gtagtcacag gatcaattgt agaaaattgt tatgtttttg ttaggaactg cactaaataa
    33001 tatatgataa gacagcaaaa acgactgacc ataatccttc ttttattagg agttgataag
    33061 agagactatt catcctgtaa tgttaaaact ctgttatata gtattcgcga ttatgcaaag
    33121 tctgtaaatg atcatgagat actaaccgag agtaatcgtt tattatctca ttgcatttca
    33181 gatagtaacg gagctttctt taaaagtagt aaatacgttc cccttaagta cttacgtaaa
    33241 aggagaatag cacgaaagat accaaatgat tagcactttg ctagtaagct tgctggtaaa
    33301 ctatattgac cgttgggttt gttttgagac tgtaatttaa attgtgtaat tgcctgtttt
    33361 tgatatgttt actccaacaa cggagacagg taaattatgg acgaaaagaa gcttaaagcc
    33421 cttgcggctg aactggctaa aggcctcaaa actgaggccg acctcaatca gttttcccgt
    33481 atgctgacga aatat
//
LOCUS       DAJTXY010000029        33207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTXY010000029 DAJTXY010000000
VERSION     DAJTXY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.029"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            78..302
                     /locus_tag="OO566_001518"
                     /pseudo
     CDS             78..302
                     /locus_tag="OO566_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
     gene            476..1245
                     /locus_tag="OO566_001519"
                     /pseudo
     CDS             476..1245
                     /locus_tag="OO566_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1278..2798)
                     /locus_tag="OO566_001520"
     CDS             complement(1278..2798)
                     /locus_tag="OO566_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6604848.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNERAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2889..3608
                     /gene="tonB"
                     /locus_tag="OO566_001521"
     CDS             2889..3608
                     /gene="tonB"
                     /locus_tag="OO566_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6604849.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(3648..4046)
                     /gene="yciA"
                     /locus_tag="OO566_001522"
     CDS             complement(3648..4046)
                     /gene="yciA"
                     /locus_tag="OO566_001522"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6604850.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4151..4690)
                     /locus_tag="OO566_001523"
     CDS             complement(4151..4690)
                     /locus_tag="OO566_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6604851.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4720..5463)
                     /locus_tag="OO566_001524"
     CDS             complement(4720..5463)
                     /locus_tag="OO566_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6604852.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(5491..5580)
                     /locus_tag="OO566_001525"
                     /pseudo
     CDS             complement(5491..5580)
                     /locus_tag="OO566_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5820..6458
                     /gene="ompW"
                     /locus_tag="OO566_001526"
     CDS             5820..6458
                     /gene="ompW"
                     /locus_tag="OO566_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6604853.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(6518..7024)
                     /locus_tag="OO566_001527"
     CDS             complement(6518..7024)
                     /locus_tag="OO566_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6604854.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDCVEAKK"
     gene            complement(7070..7570)
                     /locus_tag="OO566_001528"
     CDS             complement(7070..7570)
                     /locus_tag="OO566_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6604855.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(7656..7835)
                     /locus_tag="OO566_001529"
     CDS             complement(7656..7835)
                     /locus_tag="OO566_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6604856.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(8216..9022)
                     /gene="trpA"
                     /locus_tag="OO566_001530"
     CDS             complement(8216..9022)
                     /gene="trpA"
                     /locus_tag="OO566_001530"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6604857.1"
                     /translation="MERYESLFAQLKERKEGAFIPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(9022..10215)
                     /gene="trpB"
                     /locus_tag="OO566_001531"
     CDS             complement(9022..10215)
                     /gene="trpB"
                     /locus_tag="OO566_001531"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6604858.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(10227..11588)
                     /gene="trpCF"
                     /locus_tag="OO566_001532"
     CDS             complement(10227..11588)
                     /gene="trpCF"
                     /locus_tag="OO566_001532"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6604859.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(11589..13184)
                     /gene="trpD"
                     /locus_tag="OO566_001533"
     CDS             complement(11589..13184)
                     /gene="trpD"
                     /locus_tag="OO566_001533"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6604860.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(13184..14746)
                     /gene="trpE"
                     /locus_tag="OO566_001534"
     CDS             complement(13184..14746)
                     /gene="trpE"
                     /locus_tag="OO566_001534"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6604861.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDNALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(14768..14860)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            15019..15900
                     /gene="yciV"
                     /locus_tag="OO566_001535"
     CDS             15019..15900
                     /gene="yciV"
                     /locus_tag="OO566_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6604862.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            15897..16517
                     /locus_tag="OO566_001536"
     CDS             15897..16517
                     /locus_tag="OO566_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6604863.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            16618..17490
                     /gene="rluB"
                     /locus_tag="OO566_001537"
     CDS             16618..17490
                     /gene="rluB"
                     /locus_tag="OO566_001537"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6604864.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(17530..18120)
                     /gene="cobO"
                     /locus_tag="OO566_001538"
     CDS             complement(17530..18120)
                     /gene="cobO"
                     /locus_tag="OO566_001538"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6604865.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(18117..18875)
                     /locus_tag="OO566_001539"
     CDS             complement(18117..18875)
                     /locus_tag="OO566_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6604866.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            19095..20144
                     /gene="sohB"
                     /locus_tag="OO566_001540"
     CDS             19095..20144
                     /gene="sohB"
                     /locus_tag="OO566_001540"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6604867.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKETKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(20180..20431)
                     /locus_tag="OO566_001541"
     CDS             complement(20180..20431)
                     /locus_tag="OO566_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6604868.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            20811..23408
                     /gene="topA"
                     /locus_tag="OO566_001542"
     CDS             20811..23408
                     /gene="topA"
                     /locus_tag="OO566_001542"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6604869.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPSKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            23618..24592
                     /gene="cysB"
                     /locus_tag="OO566_001543"
     CDS             23618..24592
                     /gene="cysB"
                     /locus_tag="OO566_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6604870.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            24887..25051
                     /locus_tag="OO566_001544"
     CDS             24887..25051
                     /locus_tag="OO566_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6604871.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLTVFLGSALFWVV
                     VALLIWKVWG"
     gene            25054..25221
                     /locus_tag="OO566_001545"
     CDS             25054..25221
                     /locus_tag="OO566_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604872.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            25335..25430
                     /locus_tag="OO566_001546"
     CDS             25335..25430
                     /locus_tag="OO566_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604873.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            25594..28269
                     /gene="acnA"
                     /locus_tag="OO566_001547"
     CDS             25594..28269
                     /gene="acnA"
                     /locus_tag="OO566_001547"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6604874.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(28333..28923)
                     /gene="ribA"
                     /locus_tag="OO566_001548"
     CDS             complement(28333..28923)
                     /gene="ribA"
                     /locus_tag="OO566_001548"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6604875.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYADISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            29093..29857
                     /gene="pgpB"
                     /locus_tag="OO566_001549"
     CDS             29093..29857
                     /gene="pgpB"
                     /locus_tag="OO566_001549"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6604876.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWTLVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            30006..30314
                     /gene="lapA"
                     /locus_tag="OO566_001550"
     CDS             30006..30314
                     /gene="lapA"
                     /locus_tag="OO566_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6604877.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            30321..31490
                     /gene="lapB"
                     /locus_tag="OO566_001551"
     CDS             30321..31490
                     /gene="lapB"
                     /locus_tag="OO566_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6604878.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            31683..32420
                     /gene="pyrF"
                     /locus_tag="OO566_001552"
     CDS             31683..32420
                     /gene="pyrF"
                     /locus_tag="OO566_001552"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6604879.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNAHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            32420..32746
                     /gene="yciH"
                     /locus_tag="OO566_001553"
     CDS             32420..32746
                     /gene="yciH"
                     /locus_tag="OO566_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6604880.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(32872..33090)
                     /gene="osmB"
                     /locus_tag="OO566_001554"
     CDS             complement(32872..33090)
                     /gene="osmB"
                     /locus_tag="OO566_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6604881.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVISHQVGK"
BASE COUNT         8423 a         8024 c         8437 g         8323 t
ORIGIN      
        1 gggttacccc caaacgaatc ggaaaatcga tactggaaaa actctaactc ggtggccagt
       61 ttttgttgac cacttcagtt ccacagtttt cacctgaagc attaaataat ctgaaaggtc
      121 gagtggaaac attcacacaa gatttaaagg ttatcaaacc gtcagtaaat attcagcctc
      181 agacaatcaa tatcacaact cagacggtac ttgatggaaa ggttattgat gaaagaacca
      241 catctcatat taatcgtatg caggaagata ccttgattag ctcagcttac ccagaggaat
      301 aaacaatgtt agaactacta atatttattg ccacacatgg caatctatta atctttatct
      361 agtatgacga ttcaactatg aatagtcgaa tgctacgtga tttcttctga gcagatacca
      421 acatcaaaaa ttagatttca cgtagctcac gcttaacaga ataggaataa caacaatgtt
      481 tgacacaaag caaattagat actcaggtaa agatggtatc tattttcacc ttagagataa
      541 tgtagaatct tttcttgcct taacagctac tgaacagatg gactatgact caccgatgat
      601 ggtgacaacc aggctatgca atcggggcaa actatcactg ataatgttca gagagcacct
      661 aagaccatct ctattagcgg tgttgtagtg gttgattcga cgggttcagt tctattaacc
      721 agacaggggc aacttgtaga gacctttatt tctacggttg aacaatggcg agaccagaag
      781 caaatcatca ctattgtttg taaagatgga atatctatca ctgatagtat cattactcag
      841 tttaaagcga gtaaagagca taccattgct aatggtcttc gtattcagat gaccttccaa
      901 ggtgtagaat ttcgttcaat cgtagggcaa actgatattt cagcagctac tggcaagacc
      961 gctaccacga atgatggtgg tgctaccagt aaaaagaaca ctggcaacac tacaacgagt
     1021 cttggaagcc ctatgctgac ctgtaaagag ctttttagct attcggcaag tgagttatca
     1081 gatgaagcgc taaaagcccg tgtaacgtgc tctaagagcg ttagcgttaa aaatggtgag
     1141 agcaccttca cagcctcagc taatgagcga gcttcaaaag tgcttaagtc gggtaatgct
     1201 ttacggaagt actctgtgaa tccgaataag aagggcactt attgagacat aaaaaggggc
     1261 ttcatagccc ctgaaaatta cagttgataa gcatcaatat ccagtagctc tttatcagca
     1321 atttcaaaga ttggtgaccc atcatttagc ggtagcgggt ctgtaggtat ggtgatttca
     1381 gtgtcgttct gtagcgttat agttactgaa cccttaccca atgtaagccc ttttagatgt
     1441 ttggacaaaa ttaattgaca ctcgacacga cccgctttag ttgtcaggtc aatatcttta
     1501 agctggggta gttccttatc agtgagtgaa gccttacgct tcatagtatc aatcgtggtc
     1561 tgtagttctg tgcgcttctg gttaagttcc ttaagctgaa tttttagttc atcaatatca
     1621 tccatctcaa gaagtagggc ggtagctttc ttgattctgg ctgtgacaat ttcaacctca
     1681 tgctgtaacg ctgcaatctt cctctctgat tctccatcat tccccacgtc aatcatcccc
     1741 agaaaattat caatcatcca tcggtcaaac ggtttacgct tcaacggtgg aacatcacag
     1801 cgaccgtcta agtggttatt gcacctgtag accccagcat acttattctt tgttccggta
     1861 ggatggacac tagccccaca ctgacaccta aacagacctt tgaagatgtt aatcgttagt
     1921 gggttgtcac ttgcaggtgt acggccttta cgattcttat caagaatctc ttgagcttta
     1981 ttgaacgttg cagcatctat agcggcttcg tagtagttcg gtatagcggg gcggtcattg
     2041 cgctgtgatg ctggtagtgt acctatcgtt gtctctgacc ttaagatagt acgcacagaa
     2101 tgaacgctaa atccattagc atctttacgt cgtgagaatg aactaatgtt attagcctgt
     2161 aggtgacgtg caatgttatg cagactttca ccatctagca gcagcttgaa aatcaagcgt
     2221 acagtttcag cacgttcatt gagtttaaaa gtctcagggt caatccatcc gggaacattc
     2281 cttgttaaac gttgttcacc agcaagggca agctttgcac gttgttccca tttctgacta
     2341 acacggaaag acttctgttt actctcctga tgcgctatgt agcttttaac ccgctgaatg
     2401 attgagattc ctaaatcatc ggatagagca tcccttgtgt agattagccc atcctgtaga
     2461 gtgtgaatct cgataccatg agcaataatg cgattcatca aggcttctgt ttcaagatgt
     2521 tcaaggcggc ctatgcggtc gatagcttcg actagtaaaa ttgtcccttc tggaaactcc
     2581 cctgattcaa tgcgggagag catatcccca aattgccctg ataagtgttt acctttgtaa
     2641 ccggatagac cttcatcatt gaaagatgcc actacctgag catcgctatt cttccttaac
     2701 cactgtttga atagctcatt ttgtcgtctg gttgaatcag caccttcagc accttgtgtg
     2761 gctgatgaga atctcaagta tgttattacg gttctcacgg tgataaactc ccatgcaatt
     2821 gattatcatt taaagatgaa cttataaacg ttatggctaa tgctacagtg ccgaatagct
     2881 acaatacaat gccccttgat ttacctcgcc gcttcccctg gccgacgtta ctttcggtct
     2941 gcattcatgg tgctgttgtg gcgggtctgc tctatacctc ggtacatcag gttattgaac
     3001 tacctgcgcc tgcgcaacca atttctgtca cgatggttgc gcctgctgat ctcgaaccgc
     3061 cacaagccgt tcagccgcca ccggagccgg tggtagagcc agaaccggaa cctgagccga
     3121 tccccgaacc gccaaaagaa gcaccggtgg tcattgaaaa gccgaagccg aaacctaagc
     3181 caaaaccgaa gccggtgaaa aaggtacagg agcagccaaa acgcgatgtc aaacccgtag
     3241 agtcgcgtcc ggcatcaccg tttgaaaata cggcacccgc gcgccccaca tcaagtacgg
     3301 caacggctgc aaccagcaag ccggttacca gtgtggcttc aggaccacgc gcattaagcc
     3361 gtaatcagcc gcagtatccg gcacgagcac aggcattgcg cattgaaggg caggttaaag
     3421 ttaaatttga cgtcacgccg gatggtcgcg tggataacgt acaaatcctc tcagccaagc
     3481 ctgcgaacat gtttgagcgt gaggtgaaaa atgcgatgcg cagatggcgt tatgagccgg
     3541 gtaagccagg cagtgggatt gtggtgaata tcctgtttaa aattaacggc accaccgaaa
     3601 ttcagtaagc agaaagtcaa aagcctccga ccggaggctt ttgactatta ctcaacaggt
     3661 aaggcgcgag gttttccttc aggatcaacc gcgacatact taaataatgc ttctgtcgct
     3721 ttatagcgtt gcccgattgg ttcagacgct acttttttca cccacacttc aatattaatg
     3781 ctgaccgatg tcgtcccttt ctggacacag cgtgcatagc agcacaccac atcgccgacc
     3841 gcaaccggcc gtaagaaagt cattccttca acccgcacag tcactacgcg accgtgggca
     3901 atttcttttg ccagaatagc gccgccaata tccatttgtg acattaacca accaccaaag
     3961 atgtcaccat tggcattggt atcggcgggc atggctaaag tacgtaaaac aagatcgccc
     4021 tgagggacgt tatgtgttgt agacatggta aaaccgactt aaaaggaaaa tcacaggcgc
     4081 gatgctacta tgattttcct gaggagaaca gaggagatac agcgccagcc ccgaaggact
     4141 ggcagtctgg ttaggattta tcttcctgcg gcatgtggcg gtagatatag ataccgctta
     4201 acaatgtgaa gattaaggta agggcggtca ggccaaagac tttaaagttg acccaaatat
     4261 tttgcggcag ccagaatgcg atgtagatgt ttgccagacc gcaaaggata aagaaaacag
     4321 cccaggccag atttagcttc gaccataccg gttgcggcag cgtgagttct ttacccagca
     4381 tccgctgaat tagcggcttt ttcatcaccc attggctgac taacagggct accgcaaaca
     4441 gagcataaat gactgtaacc ttccatttaa taaactcatc attgtggaag aacagcgtca
     4501 agccaccgaa gacgaccacc agaacaaaag tgatcagggc catcttctca accttacgaa
     4561 agcgaaccca gctatagata agcacaatcg ccgtggcgac gatcagcgct gcagtagccg
     4621 catagatgtc ataaatcttg taaaacgcga aaaagacaac cagcggtaaa aaatcaagaa
     4681 actgcttcat tttacgattc cgtaatcaaa tgcttaaggt tattggcgaa tcaacatata
     4741 caggcggaac agatagatga gcaatatggc agaaatcagg ttgctcaagg tgttcgccag
     4801 tacggcacca atttccgggg ttaatgcggc aaaagaagag gcaaaaagca gcaacagtgt
     4861 ttttgccagc aaccagctca gtactgcggg tgccaccaga cgcatattcg cccaagtcag
     4921 ccgcatactg ctacgcatcg aggcaaaaat gcccattttg tcctgtacca gcatcaccgg
     4981 tgccagggcc agtaaaatgg ccatgataat tcccggaacg accaccagca tgatgccaat
     5041 ctgtactaaa agggtagtca gaaaaatcag aataaataac ttgggcaata tcggcgcact
     5101 ggcaccaata gctcgcagcg cacagactct ctgacccgca gacaccagct ggataattaa
     5161 tattacgcct ccggcgagaa tggcgttacc gattaattct gaaaaagtgg acgccgctga
     5221 agcctgcagc aaaatttgtt gctgttccgg tgacatattc tgaaccaggt cgaacaaccc
     5281 actactgccg ctaacgggca cgccgtcatt gagctgcgca agctgtgcat cactgggtga
     5341 gaaaacatgc cctaacacca ctgtgataaa cgcacatagc aacgatacca acagaatggt
     5401 cataaattga ttacggaaga aatttccggt gtcacggtat acggactgcg ccgtgataga
     5461 catgcactct ccttgagttt tatgcaggtg ttaattagcg ggcaattgta ccctggttaa
     5521 gcctcgggtg gcagcatcag gtcttgtatg gaaaagcata tctttgtaaa gcggaggtaa
     5581 ttgctggcct tcaggcgtgg gcaaaccgcg gcaagtggcg atcgagttaa tatttgcgta
     5641 gagaaaatat ttaaaaattg atttaaatca cattaaccag gattctcaat gcaacttcta
     5701 aattaatcca gatcaataaa tggtgaatta tcatatgtaa tgtgatctat gtaggatcat
     5761 ttgttactcc aatgtaggta tattcgtcac gtttttataa ccataacgac ggagcggata
     5821 tgaaaaagtt aacagtggcg gctttggcag taacaactct tctttctggc agtgcctttg
     5881 cgcatgaagc aggcgaattt tttatgcgtg caggttctgc aaccgtacgt ccaacagaag
     5941 gtgctggtag tacgttagga agtctgggtg gattcagcgt gaccaataac actcaactgg
     6001 gcttgacgtt tacttatatg gcgaccgaca acattggtgt ggaattactg gcagcgacgc
     6061 cgttccgcca taaaatcggc acccgggcga ccggcgatat tgcaaccgtt caccatctgc
     6121 cgccaacact gatggcgcag tggtattttg gtgatgccag cagcaaattc cgtccttacg
     6181 ttggggccgg tattaactac accaccttct ttgataatgg atttaacgat catggcaaag
     6241 aggcggggct ttccgatctc agtctgaaag attcctgggg agctgccggg caggtggggg
     6301 ttgattatct gattaaccgt gactggttgg ttaacatgtc agtgtggtac atggatatcg
     6361 ataccaccgc caattataag ctaggcggtg cacagcaaca cgatagcgta cgcctcgatc
     6421 cgtgggtgtt tatgttctca gcaggatatc gtttttaatt ccgcacaaaa acgaccccgt
     6481 aatatacggg gtcaataagg acatggtata aagggtatta tttcttcgct tctacgcaat
     6541 cagtttcaga acgaattaaa aatttctcag ttgtttgcgg aatatgctga atcagccagt
     6601 cggccatttg cttttcctca tttaaaatcg cttcgatgat cggaattgag actgtatcac
     6661 cggcattttt tgctgctgct aatagtgagg tgtaacaggc gatttcaaat tgctcgaaga
     6721 catatccgct aatagagcct ttaactattt catcagaagg gaatatacca ccgattgact
     6781 gcccaagcgc agccatttta ctcatggaat ctttaatgac tgaacgtgaa atgtcattac
     6841 gatcaagaat agtttccagt tgaacaatct ggtttttggt ttcactaaga tgttgttcaa
     6901 tacgagcgcg tagttcagga taattatcta tacggctggc catggattca agcatagatt
     6961 cggcttgctt ttccattgcg tgggcgtcac gtaaccagtc atgataatgt tcaatacgat
     7021 tcatgaaaat gtcctcttta taaaatgact aaaagttaaa attcatattt caggctttat
     7081 tttcggcttt cttatttacg ttattaaggg ccagatcagt cagtttgatg tcggtggcct
     7141 tttcttcttc cagggtttct ttcagaagct tcgctgcttt acggtaacct aattgttcag
     7201 ccagcgtcgc taatgtcccg taactggcaa tctcataatg ctcgactttc tgtgctgcgg
     7261 caatcagtgc ggcatcacgc acttcgtttt tctcggtact ttcgatgacc tcattagctt
     7321 cttcaataag accttccatt gccacacatt tcatgcgctt aattttcaga tttgattctg
     7381 attccacaac ttgatcaatt cgttcaatct gtccatgagt ttcctcgagg tgcgcatgaa
     7441 aagcctgact taatttttca tttgatgttg ctcttgcgag ttttgccagt gcccggggta
     7501 attgtttttc tgcgctgtag gtatctgaaa gcagttgaat aaatacatct tcaatggtct
     7561 tcatattcat atttttctcc agtgaaatca ctgcgagcca tacggcccgc agatgcaatt
     7621 atctttatca gctgatatta ataattaacg gtgaatcagg atttgtctga tttattacca
     7681 ccgctttgtt gaccgccttt tttacccgct tcagatgcgc gttgcggatc atttttaaaa
     7741 ttaccgccgc tatgctgacc gcctttacgg cctgcgtcgg atgccttctc acggtcttcg
     7801 gcgaaatttc ctgaaccacc acgatgttcg gccatgttat ttctcccgtt gcgttgcatt
     7861 gtttcattaa tatgagtgtt gtgtgtcgac actcattaaa attagtcgct aatgagaatt
     7921 agtcaaatta agcgcaacga gaagatagag ggaaaatata ttttgaggaa cattctggat
     7981 atattaacaa ttacctgagg aataaatgac ttagagaaaa ttattgattt tactggtgtt
     8041 atgttgcggg attaatttgt ttgattatat tactgttggg cggaaaaatg acgtaagttg
     8101 acgttcgacc ggggtaagcg aaacggtaaa aagataaata ttaaatgaat ttaggatttt
     8161 tccggcttca ttaaagaaag ttaaaatgcc gccagcggaa ctggcggctg tgggattaac
     8221 tgcgcgtcgc cgctttcatc ggttgtacaa aaactttcag tgccgccagc attttctctg
     8281 gctcattaat atgttgctcg atgattttaa caatggccga accagaaatc gcgcccgcag
     8341 ctcctgcatc aatcgctgct tttacctgat ccggggcgga aataccaaac ccctgcaatg
     8401 gaggtgcagc gttgtactct ttcagcttcg caaccagatg attgaggggt aacgcggcgc
     8461 ggttttctgc gccggtcacg cccgctcgtg acagcaaata tgtgtaacca cgaccgtaag
     8521 aggctatctg gcgcagcagg tcgtcatcgg catttggcgg gcagatgaag ataggtgcga
     8581 cattatggcg caacgcggcc tggcggaagg gcgcggactc ttcaactggc acatcggcaa
     8641 ccagcaccga atcgacgccg actttctcgc actgggcata aaactcatca atgcctttgt
     8701 taaacaccag attggcatac atcaacaggc caatgggaat ggtcgggtgt ttctggcgaa
     8761 tcagtgccag catttcaaaa cattgtgccg gagtcacacc tgccgcaaag gcgcgcagag
     8821 tggcgttttg aatggtcggg ccatccgcca gtgggtcgga gaaggggata cctaactcca
     8881 gcgcgtcagc accggcttca attagcgtat cgataatttt caatgactgc tcaatgcccg
     8941 gatcaccgag cgtgacgaaa ggaatgaatg cgccttcttt gcgctccttc aactgggcaa
     9001 acagagattc gtagcgttcc atcagatttc ccctcgtgct ttcaaaatat cgtgaacggt
     9061 gaagatgtct ttatcgccgc gaccggaaag gttaaccacc agtagctgct ctttatccgg
     9121 gttttcgcgc atcattttca gcgcatgggc cagggcgtgg gaggattcca gcgccgggat
     9181 gatcccttcg tgcaggcaca gcgttttgaa ggcttcaagg gcttcatcat cggtaataga
     9241 cacgtaatca gcgcgtccag tgctgttaag atacgcgtgt tgtgggccga cagacgggaa
     9301 atccagtccg gcggagatgg agtaagattc ttcaatctgc ccgtcttcgg tttgcatcat
     9361 cggcgctttc ataccgaaat aaatgcccac gcgaccatgt tttagcggtg cgccgtgctc
     9421 gccagtttcg ataccgtgac caccaggctc cacaccaatc aggccgacgt tggtttcatt
     9481 gatgaaatca gcaaacatgc cgatggcatt cgaaccgccg ccaacacagg cgataacggc
     9541 atccggcagg cgaccttctc tttccagaat ctgcgctttg gtttcttcgc caatcatccg
     9601 ctgaaactca cggacaatgg tcggataagg atgcgggcca gccgcggtgc ccagcatata
     9661 gtgcgcggtt tcataactgc cggaccagtc gcgtagcgcc tcattacagg catctttcag
     9721 cgtggcggaa ccgctatgta ccgggatcac ttccgcaccc attaagcgca tacgaaaaac
     9781 gttaggcgac tggcgttcaa cgtctttggc acccatataa atacggcatt tcaggccgag
     9841 cagggcgctg gcaagggccg acgccacgcc atgctgaccg gccccggttt cggcgatgat
     9901 ttcggtttta cccatccgct tcgccagcaa cgcctgcccc agcacctggt tggttttatg
     9961 tgcgccgccg tgcagcaaat cttcacgctt gagatacagc gtggtgttcg tcccggctgt
    10021 aatgttctgg catttggtca gtgcggttgg acgcccggca tagtttttca gcaggtcgtt
    10081 gaactgggct tgaaattcag gatctttttg cgcgctgaca aaagcttctt ccagctggcg
    10141 cagagcaggc atcaggattt gtggcacgta catgccgcca aactcaccaa aataggggtt
    10201 aagtaatgtt gtcattgttc ctttccttaa tatgcgcgca gcgtctggaa aaccgaggcc
    10261 aaaagacgtg cgtctttgat gcccggttgc gactctacag cagaattaaa atcaagtccg
    10321 gcgcagccgg tttgtgccgc ttccacgcag ttatctgcgc ctaagccccc cgccagcaga
    10381 acgttgccaa gcgattgacc atttaatagt gaccagtcaa aacgttgccc gcttccaccc
    10441 tggccgttgt ctaaaacata tttatcaacg tgctgaaact cgcgggcggg cagggtttca
    10501 ccgacgctta atgctttcca gatggcaaca tgtgctggca gagcttcacg cagcgtatcg
    10561 atatacagct gatcttcatt accatgcagt tgcactgccg ccagcgataa caccttagct
    10621 ttgtccacca catcggcaat atcgtgattg cggaacacgc caacatactg caacggtgcc
    10681 gcagccatca cttcctgcgc ctgttcaacg ttgacgcaac gcggtgatgt cgcaacaaaa
    10741 atcaacccac cgtaaatcgc gcccgcgtca taagctgctt tagcatcttg cccacgcgtc
    10801 aggccacata ctttattctc acccagcaac acccggcgca cggcggcgtg caaatcgtca
    10861 tgggccatca acgccgaacc aatcagaaaa ccgttagcga agtggcttaa ctcgcgcacc
    10921 tgagcgtaag tattgatgcc ggattcgctg attaccgtta cgttgtgccc cagtttcggc
    10981 gcaagctcgc gggtacggtt gagatcaatc gacaaatcac gcagatcgcg gttgttgatg
    11041 ccaacgacct ttgcccctaa tgcaatggcg cgctccagtt cctcttcatt actgacttcg
    11101 gtcagcaccc ccatctccag actgtgagcg acggctgcaa gctggcgata ttggtcgtca
    11161 tccagtactg aaagcattaa taagcaggca tcggcctggt aatagcgcgc cagatagatc
    11221 tggtaagggt cgataatgaa gtctttacat aaaatcggct gcggggcgat ttggctgacg
    11281 atgaggagga aatcaaagct cccctgaaaa tatttctcat cagtcagcac cgaaattgcc
    11341 gaagcgtaat gtttataaat ggcggcaatg cgtgccggat cgaaatcatc acggatcacg
    11401 ccttttgacg gcgacgcttt tttacactcc agaataaacg ccgtgcgtgc accttgtagc
    11461 gcatcataaa aatgtcgtgt gctcggctga acctcattct gaaaactggc cagcggttgc
    11521 tgctgtttgc gggtttctac ccaaatcgcc ttgtctgcga cgattttcgc taaaacggtt
    11581 tgcatcattt accctcgtgc cgccagcgcg gtaactctgt cgtaagcgga accactgcgc
    11641 agtacctcaa gaacggtttg cgcattggct tgcagatctt catggccatg caggcgcatt
    11701 aacatggcga cgttcgcagc gacggctgct tcatgggcgg cgtcgccttt accttgtaac
    11761 aagcgtgtta aaatgtcacg gttttcttca ggtgttccgc ctgccagttg ctcctggtgg
    11821 tagggagtca ggccaaaatc ttcagcggtc aattgatagc tcttaatttt gccgtcatgc
    11881 agctcggcaa cgattgtcgg cgcgtgtaat gaaacttcat ccatcccgcc gctgtgcacc
    11941 accgccgcgc gttgataccc cagcacgcgc aaggtttcgg caatcggcag caccagttcc
    12001 ggactataaa caccaattaa cgccagcggc ggatgcgccg ggttaatcaa tggccccagc
    12061 acattgaaca gggtgcgggt tttcagttgc tggcgaaccg gcatcgcgtg gcggaatccg
    12121 gtgtgatatt tcggcgcaaa gaggaaacat acgcctaact cgtccagcgc ctggcgcgat
    12181 ttattggcgt tcatatcaag attaataccg aatgccgcca gcagatccga cgagccggat
    12241 ttactggaga cgctgcggtt accgtgtttc gccactttca gcccacaggc cgcggcgaca
    12301 aacgcactgg cggtagaaat attgatactg ttgctgccgt caccgccagt accgacgata
    12361 tcagcaaaca gataatccgg gcgcgggaac ggcgctgcgt tttccagtag cgcggttgct
    12421 gccccggcga tctcgttcgg gtgctcacca cgaattttca tgctcaccag cgccgccgcc
    12481 agttgttccg gcttcagctc gccacgcacc accgctgaaa acagctggtg gctttcttgt
    12541 tggctaagcg tctgcgcctg atacagtttt tccagaatcg gttgcagcgt gttggctggc
    12601 tctagtttct gctgcgccca ggccagcgtt tgttccagca ggcgagcgcc ctgggtggtg
    12661 agaatggatt ccggatggaa ctggaatcca caaacgcgat ccgcatcgtg acgtactgcc
    12721 atcaccatgc cattaaaatg ggcgttgatg gttaaaccgg ccggaatgtt actgccaacc
    12781 agcgagtgat aacgcgccac cggcagcggg tttgttaatc cggcaaacat cgcctgaccg
    12841 tcatgttcaa tgctcgacgc tttaccgtga agaatttcgc ccgcctgacc gacatagccc
    12901 ccgtaagctt cgacaattgc ctgatgtccg aggcaaatgc caataatggg cagcttgcca
    12961 cgcaagcggg tgaggagttc cggcatacaa ccggcttcgc tcggcacacc ggggccagga
    13021 gaaagcatca gtaccggatt gctcatcgtc gccaggcgtt caattaaggt ctgcgccgga
    13081 atatggttgc ggtaaatcac cacgttatga ccattgctgc gcaactgatc tgccaggttg
    13141 tacgtaaaag agtcgatatt atcgagcagc agaatgtcag ccatcagaaa gtctcctgtg
    13201 catgatgcgc ggtggcaata gcgcgcagta cagcgcgggc tttgttacgg gtttcgtcgg
    13261 cttccgactg cggaacagaa tcaaggacta caccagcacc cgcttgcacg gtggcgatac
    13321 cgttttccac cagcgccgag cggatcacaa tgcaggtgtc gagatcgcca tgcgcggtga
    13381 aataacctac cgcgccgccg tagctgccgc ggcgacgacc ttccgcctcg gcaattaact
    13441 gcatagcgcg tactttcggc gcaccgctta acgtccccat attcatacag gcgcgataag
    13501 cgtgcagggc gtcaagatcg tgacgcagtt cgccgactac gcgagagacg aggtgcatca
    13561 cataggaata acggtcaact ttggtgagat cggcgacgta gcggctgcca ggggtgcaaa
    13621 tgcgtgccag atcattacgg gcgagatcaa ccagcatcag atgttcagac agctctttat
    13681 ggtcagtacg catttccagt tcaatacggc tgtcgagatc tctgtccagt gaaccatcgg
    13741 cgcgacgacc gcgtgggcgt gttccggcaa tcgggtagat ctcaatctgg cggctggtgg
    13801 catcatactt gagcgagctt tccggcgacg cgccaaatag ggtgaaatca ttatcctgca
    13861 taaaaaacat gtacgggctg ggattactct ttttcagcac gtaataggcc gccagcggtg
    13921 acgggcaggg cagagagaaa cggcgagatg gcaccacctg gaaaatttct ccagcgcgaa
    13981 tcgctttttg caacaaacgc actacgccac cgaactcttc atcgctctga ttacattcac
    14041 aacgcatatg cggcacggaa accactggca gcggcggcgc ggcttcggtc agttgctgac
    14101 gtagttcgtt caggcgagca gtgagacgtt gtttttcttc ttcattcgga gcaaacaggc
    14161 tggcctgaat acgggtgctt tttttctgat ggtcaatcac catcagcgtt tcagcgagat
    14221 aaaaacagaa atcagggcag ttattttccg ctgacagttg cggtaaatct tcaaatcccg
    14281 ccacaaggtc ataagagaac aggccgccga agaacattgc ttctcgttct tccttcggta
    14341 cattcaacag attctgcaat aaacggaaag cgtcaaaaac cgaaagggag cataagcggg
    14401 cgtcttcatc cagcagtgga ctgacagggg ggaagcgcag cacacggcag tttggtgatt
    14461 gttcactttc cacacccgca ggcagggcgt tatccagtag tgccagcagg gcttcgccat
    14521 tgccggaaag tgcctgaatt gtgacggtgt cacctaatgc tgtaatgtgc agcgcattgt
    14581 ctatcagcag caggcttttt aaatcatctt tgctgtcgat atctgcggat tccagcagca
    14641 gcgttgccgg acgattccca cacaactggt gaaaaagcgc agtcgggttg tcgcgataag
    14701 cgccttcgca ggttagcagt tcgagagtcg gtttttgtgt ttgcattgtt attctctaat
    14761 tttgttcaaa aaaaagcccg ctcattaggc gggctgggta tctgattgct ttacgcatgg
    14821 tgaatacact gcccgtttca ggaagtggcc accaaccttt cagtacgaaa attgctttca
    14881 ttgtcgatac catttttacg tgaacttgcg tactagccaa ctggttcgat gattaattgt
    14941 caacagctca tttcagaata tttgccagac ccgttatgat gtcggcgcaa aaaacattat
    15001 ccagaacggg agtgcgcctt gagcgacacg aattatgcag tgatttacga cctgcacagc
    15061 cataccacag cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgag
    15121 atgcgcgtcg gcaccctggc gatcaccgac catgacacca cagctgcaat cgcgccagca
    15181 agagaggaaa tttcacgttc gggactggcg ctgaatctta ttcccggcgt ggaaatttcc
    15241 acggtctggg aaaatcatga aattcatatt gtcgggctga atattgatat tactcatccg
    15301 ctaatgtgtg agttcctcgc gcagcagaca gaacggcgca atcagcgggc gcagcaaatt
    15361 gccgaaagac ttgaaaaagc gcaaatacct ggggcgctgg aaggcgcaca acgactggcg
    15421 cagggcggcg cagtgacgcg cggtcatttt gcgcgtttcc ttgtggagtg cggcaaagcc
    15481 agttcaatgg cagatgtctt taaaaagtat ctggcgcgcg ggaaaaccgg atacgttccg
    15541 ccacagtggt gtacaataga acaagctatt gatgtcattc atcattctgg cggtaaggcg
    15601 gtgctagctc atcctgggcg gtacaatctt agtgctaaat ggctgaaaag attggttgcg
    15661 cattttgccg aacaccacgg cgacgcgatg gaagtcgcgc agtgccagca atcgcccaat
    15721 gaacgcaccc agctggccac acttgcgcgt cagcatcatt tatgggcatc acaaggatct
    15781 gattttcatc agccatgccc gtggatcgaa ctgggtcgta aactctggct gcccgcaggc
    15841 gttgaaggcg tctggcagct atgggaacag ccgcagaaca ccacagagag ggaattatga
    15901 gccagttttt ttatattcat cctgataacc cgcagcaacg tctgatcaac caggcggtgg
    15961 agatcgtgcg taaaggcggg gtgattgtct atccaactga ttccggctat gcgctcggct
    16021 gtaaaattga agataaaaac gccatggagc gtatttgtcg tattcgccag ctgccggacg
    16081 gtcacaactt taccctgatg tgtcgcgatc tttctgaact ctcgacctat tcattcgtgg
    16141 ataacgttgc gtttcgtttg atgaaaaaca acacgccggg caactatacc ttcatcctga
    16201 aggggacgaa ggaagtgcca cgccgtctgt tgcaggagaa acgcaaaacc atcggtatgc
    16261 gtgtgccttc taacccgatt gcgcaggcgt tgcttgaagc actgggcgaa ccgatgcttt
    16321 ccacttcgct aatgctgcca ggcagcgaat ttaccgaatc cgatcccgaa gaaattaaag
    16381 accgtctgga aaaacaggta gatttgatta ttcacggcgg ctatctcggg cagaaaccaa
    16441 caacggttat tgatcttacc gatgatacgc cggtcgtggt gcgtgaaggc gtaggtgatg
    16501 tgaagccttt cttataagtg cgtaactctg tatactacgc ggccttaaaa tggcgctata
    16561 cggcgccatt gattctcctg ttcgacgcct gtgaaggcga caccaaaagg aagctctatg
    16621 agcgaaaaat tacagaaagt gctggcccgc gctggccacg gctcccgccg tgaaatcgaa
    16681 tctattattg aagccggtcg tgtgagtgtt gatggcaaaa ttgctaaact cggcgatcgc
    16741 gttgaagtta cccctgggct gaaaattcgt atcgatggtc atctgatttc ggtacgtgag
    16801 tccgctgaac aaatttgtcg cgttctggcc tattacaaac cggaaggtga gttgtgtacc
    16861 cgtaacgatc cggaagggcg tccgacggtg tttgaccgtc tgccaaagct gcgtggcgca
    16921 cgctggattg ccgtaggtcg tctggacgtt aacacctgtg gtctgctgct gttcaccacc
    16981 gatggtgaac tggcaaaccg tttaatgcat ccaagccgtg aagttgaacg tgaatacgcc
    17041 gtgcgtgtat ttggtcaggt tgacgacgcg aaactgcgtg atttgagtcg tggcgtgcaa
    17101 ctggaagatg gtccggcagc ttttaaaacc atcaagttca gcggcggcga agggatcaac
    17161 cagtggtaca acgtgactct gaccgaaggc cgtaaccgtg aagttcgtcg tctgtgggaa
    17221 gcggttggtg tgcaggtgag ccgcctgatc cgtgttcgtt acggtgatat cccactgcca
    17281 aaaggtctgc cacgcggtgg ctggacggag ctggacctcg cccagactaa ctatctgcgc
    17341 gaactggtgg agcttccgcc agaaaccagc tctaaagtcg ctgtagaaaa agaccgtcgt
    17401 cgcatgaagg ctaatcagat tcgtcgtgcg gtgaaacgtc acagtcaggt gagcggcggt
    17461 cgtcgttctg gcgggcgtaa taacggttaa tcaaagtatg ccggacgtca tatccggcat
    17521 ttttacagat taataatcaa tccctatttg cgccttaaca ccggcatcaa acgcatgtct
    17581 gatggggcgc agctcactta ccgtatccgc cagttcaaga atatcccgat gacagcctcg
    17641 acccgtgata atcaccgtct gttgatgtgg acgttcattt aacgcctgca ccacttcttc
    17701 cagtggcaaa tagtcatacg ccaccatata cgtcagttca tcaagcaaaa ccatatccag
    17761 tgaggaatca gcaagcatcc gctttgcatg ttgccagact tcgcggcagg cggcggtatc
    17821 agactcgcgg ttttgtgtat cccaggtaaa gcccgttgcc atcacctgaa actcaacgcc
    17881 atgtggctcc agcagattgc gttcgccatt aggccaggtg cctttaataa actgcacgac
    17941 gcccactttt tttccgtgac caactgcgcg tgttgccgta ccaaatgccg cggtggtttt
    18001 gccttttcca ttgccggtaa agacgatgat aataccgcgt tcatcctggg cctgggccac
    18061 acgagcatct actttttctt tcactcgctg ctgacgctgt tggtagcgtt catcactcat
    18121 tgggaaattc ctggtttacg gcccggttgg gcgtcaaagg tcatgccggt tttacggcgg
    18181 ctgtcatcgc ccatcagcca gaggtagagc ggcatgatat cagcgggtgt tttaagtttc
    18241 tgtggatctt cggtcgggaa ggcgcttgcg cgcattgcgg tgcgcgtacc gcctgggtta
    18301 atgcagttga cgcgcaggcg ctgctgatat tcatcggcca gtacctgcat catcccttcg
    18361 gtggcaaatt tcgacgctgc atatgcaccc cagttggctc gtccctgacg tccaacgctt
    18421 gatgaagtaa agaccagtga accggcgtcc gatttgagta ataaaggaag cagtgcctgg
    18481 gtgagcataa aggtggcatt aacgttgacc tgcatgacgt cctgccagac ctgcggattt
    18541 tgttcgctca ttgggcaaac atcgccgagc aatccggcat tatgcaaaac accatccaga
    18601 cgcggataat taacgacaat gcgctgtgcc agttgttggc aattttcgga cgtgcaggtc
    18661 agcaaatcga gaataaacca ctgtggctga cgcccagttt cttcgtttat gtggctggct
    18721 acctgacgta atttttcttc attacggccc aacagaatca ctgtcgcacc atagcgtgca
    18781 tacgtcatcg cggcttcacg accaatccca tcgctggctc ccgtcaccag gataatgcga
    18841 tcattgagta aatcttgttt tggctggtaa tgcatggcta ctcctcaacg acgttgtctg
    18901 tcgcgctgcc agtgtacttt atgactttgg ggctttatgc ctgaaacaga agggcatttc
    18961 aatcagcctg gggcgataac accgcaaaat ggatactttg tcatactttc gctgcaataa
    19021 catctctgcg agacggctta acatgcctgt tgtaaactgt gagccaaagc gttgtttaac
    19081 caaggtgggg actcgtggaa ttgttgtctg aatatggttt gtttttggct aaaatcgtta
    19141 ccgttgtgct agcgattgcg gcgattgccg ccattattgt caatgttgct caacgtaata
    19201 aacgccagcg tggcgagtta cgggtcaaca atctcagcga acagtataag gagacgaaag
    19261 aagaactggc cgcggcgctg atggactcac atcagcaaaa acagtggcac aaagcgcaga
    19321 agaaaaagca caagcaagaa gcgaaagcag caaaagcgaa agccaaactg ggcgaggtgg
    19381 caactgacag taaacctcgc gtctgggtgc tggattttaa aggcagcatg gacgcccatg
    19441 aagtgaactc gctacgtgaa gagataacgg cggtactcgc agcattcaaa ccgcaggatc
    19501 aggttgtgct ccgtctggaa agccctggtg gcatggtgca tggttacggg ttggcggctt
    19561 cgcagctgca gcgtctgcgt gataaaaaca ttcctttaac tgttacggta gacaaagtcg
    19621 ctgccagcgg cggttacatg atggcctgtg tggcggacaa aattgtttcc gcaccgtttg
    19681 ctattgtggg ttccattggg gtggtggcgc aaatgcccaa ctttaaccgc ttcctgaaaa
    19741 gcaaagatat tgatatcgaa ctgcacaccg ccgggcagta taagcgcacg ctgaccttgc
    19801 tgggtgaaaa taccgaagaa gggcgggaga aattccgcga agagctgaac gaaacgcatc
    19861 agttatttaa agattttgtg aagcgtatgc gtccgtctct ggatattgaa caggtggcaa
    19921 cgggtgaaca ctggtacgga caacaggcgg tagagaaagg cctggttgat gaaatcaaca
    19981 ccagtgatga agttattctt agcctgatgg aaggccgtga agtggttaat gtacgctata
    20041 tgcagcgtaa acgactcatt gaccgattca ccggcagcgc ggcagagagc gccgatcgat
    20101 tgttgctacg ctggtggcag cggggtcaaa agccattgat gtaaaagaca aacgcgaggc
    20161 taagacctcg cgttttgctt taatcaacca gatgatattt ttctgaaagc acatgggcca
    20221 ggtgtttgaa catattaaac accgcggtgc ttttggctgt tggcaatcct tgttcatcta
    20281 aaaagtagtc gccggtaaat accagcacgc cattacgctg cgtgacgccg gtggcttcaa
    20341 tccctgcgag cgtatcctca tgctcacgaa tgattttgtt agcctctttc aacagcgttt
    20401 cgcggtcgat gggttgtgtc tctttgttca ttatttactc cttaaacaag gacattagtc
    20461 tacgccaggc atggcttgca gacaaatata ccacgctggt ggcaagagcg ccttactggc
    20521 aactttggat tttgcatgct aataaagttg cgtatcggat tttatcaggt acagtgtgac
    20581 gctttcgtca atctggcaat agatttgctt gacattcgac caaaattccg tcgtgctata
    20641 gcgcctgtag gccaagacct gttaactcag tcacctgaat tttcgtgaac agagtcacga
    20701 caagggggtg atatccgcag agagcgagtc catatcggta actcgttgcc agtggaaggt
    20761 ttatcaacgt gcgacgcatt cctggaagaa tcaaattagg taaggtgaat atgggtaaag
    20821 ctcttgtcat cgttgagtcc ccggcaaaag ccaaaacgat caacaagtat ctgggtagtg
    20881 actacgtggt gaaatccagc gtcggtcaca tccgcgattt gccgaccagt ggctcagctg
    20941 ccaaaaagag tgccgactct acctccacca agacggctaa aaagcctaaa aaggatgaac
    21001 gtggcgctct cgtcaaccgt atgggggttg acccgtggca caattgggag gcgcactatg
    21061 aagtgttgcc tagtaaagag aaggtcgtct ctgaactgaa acaactggct gaaaaagccg
    21121 accacatcta tctcgcaacc gaccttgacc gcgaagggga agccattgca tggcacctgc
    21181 gggaagtgat tggtggtgat gatgcgcgct atagccgagt ggtgtttaac gaaattacta
    21241 aaaacgcgat ccgccaggca tttaacaaac cgggtgagct gaatattgat cgtgttaatg
    21301 cccagcaggc gcgtcgcttt atggaccgcg tggtggggta tatggtttcg ccgctgctgt
    21361 ggaaaaagat cgctcgtggt ctgtctgccg gtcgtgtgca gtcggtggcg gtccgcctgg
    21421 tggtagagcg tgagcgtgaa attaaagcgt tcgtaccgga agagttctgg gaagtcgatg
    21481 ccagcacgac tacgccatct ggtgaagcgt tggcgttgca ggtgactcat cagaatgaca
    21541 aaccgttccg tccggtcaac aaagaacaaa ctcaggctgc ggtaagtctg ctggaaaaag
    21601 cgcgctacag cgtgctggaa cgtgaagaca aaccgacaac cagtaaacct ggcgctcctt
    21661 ttattacctc tacgctgcaa caagctgcca gcacccgtct tggttttggc gtgaaaaaaa
    21721 ccatgatgat ggcgcagcgt ttgtatgaag caggctatat cacttacatg cgtaccgact
    21781 ccactaacct gagtcaggac gcggtaaata tggttcgcgg ttatatcagc gataattttg
    21841 gtaagaaata tctgccggaa agtccgaatc agtacgccag caaagaaaac tcacaggaag
    21901 cgcacgaagc gattcgtcct tctgacgtca atgtgatggc ggaatcgctg aaggatatgg
    21961 aagcagatgc gcagaaactg taccagctaa tctggcgtca gttcgttgcc tgccagatga
    22021 ccccagcgaa atatgactcc acgacgctga ccgttggtgc gggcgatttc cgcctgaaag
    22081 cacgcggtcg tattttgcgc tttgatggct ggacaaaagt gatgcctgca ctgcgtaaag
    22141 gcgatgaaga tcgtatctta ccggcagtcg ataaaggcga tgctctgacg ctcgttgaac
    22201 taacaccagc ccagcacttt accaagccgc cagcccgttt cagtgaagca tcgctggtta
    22261 aagaactgga aaaacgtggt atcggtcgtc cgtctaccta tgcgtcgatc atttcgacca
    22321 ttcaggatcg tggctatgtg cgagtagaaa atcgtcgttt ctatgcggaa aaaatgggcg
    22381 aaatcgtcac cgatcgcctt gaagaaaatt tccgcgagtt aatgaactac gactttaccg
    22441 cgcagatgga aaacagcctc gaccaggtgg caaatcacga agcagagtgg aaagctgtac
    22501 tggatcactt cttctcggat ttcacccagc agttagataa agctgaaaaa gatccggaag
    22561 agggtggtat gcgcccgaac cagatggttc tgaccagcat tgactgcccg acttgtggtc
    22621 gcaaaatggg gattcgcaca gcgagcaccg gggtattcct tggctgttct ggctatgcgc
    22681 tgccgccgaa agagcgttgc aaaaccacca ttaacctggt gccggaaaac gaagtgctga
    22741 acgtgctgga aggcgaagat gctgaaacca acgcgctgcg cgcaaaacgt cgttgcccga
    22801 aatgcggcac ggcgatggac agctatctca ttgatccgaa acgtaagttg catgtctgtg
    22861 gtaataaccc aacctgcgac ggttacgaga tcgaagaggg cgaattccgc attaaaggtt
    22921 atgacggccc gatcgttgag tgtgaaaaat gtggctctga aatgcacctg aaaatggggc
    22981 gtttcggtaa atacatggcc tgcaccaacg aagagtgtaa aaacacacgt aagattttac
    23041 gtaacggcga agtggcacca ccgaaagaag atccggtgcc attacctgag ctgccgtgcg
    23101 aaaaatcaga tgcttatttc gtgctgcgtg acggtgctgc cggtgtgttc ctggctgcca
    23161 acactttccc gaaatcgcgt gaaacgcgtg cgccactggt ggaagagctt tatcgcttcc
    23221 gcgaccgtct gccggaaaaa ctgcgttatc tggccgatgc gccacagcag gatccggaag
    23281 gtaataagac catggttcgc tttagccgta aaaccaaaca gcaatatgtc tcttcggaaa
    23341 aagacggaaa ggcgactggc tggtcagcat tttatgttga tggcaaatgg gttgaaggaa
    23401 aaaaataacc tttaattcta tcaggttttt ataaacaaag ggtcgcgaaa gcggcccttt
    23461 tttattgcat attatttttt cttcacacct atacactaag gctataaatg atatagtggt
    23521 tatagttagc acctttttta ttattaaatc gtattagtca cccgccaggt gtgacgaaaa
    23581 aacgatgttc tgatggcgtc taagtggatg gtttaacatg aaattacaac aacttcgcta
    23641 tattgttgag gtggtcaatc ataacctgaa tgtctcatca acagcggaag gactttacac
    23701 atcacaaccc gggatcagta aacaagtcag aatgctggaa gacgagctag gcattcaaat
    23761 tttttcccga agcggcaagc acctgacgca ggtaacgcca gcaggacaag aaataattcg
    23821 tatcgctcgc gaagtcctgt cgaaagtcga tgccataaaa tcggttgccg gtgagcacac
    23881 ctggccggat aaaggctcgc tgtatatcgc caccacgcat acccaggcac gctacgcgtt
    23941 accaaacgtt atcaaaggct ttattgagcg ttatccccgc gtttctttgc atatgcacca
    24001 gggcccgccg acacaaattg ctgatgccgt ctctaaaggc aatgccgatt tcgctatcgc
    24061 cacagaagcg ctgcatctgt atgaagattt agtgatgtta ccgtgctacc actggaatcg
    24121 ggctattgta gtcactccgg atcacccgct ggcaggcaaa aaagccatta ccattgaaga
    24181 actggcgcaa tatccgttgg tgacatatac cttcggcttt accggacgtt cagaactgga
    24241 tactgccttt aaccgcgcag ggttaacgcc gcgtatcgtt ttcacggcaa cggatgctga
    24301 cgtcattaaa acttacgtcc ggttagggct gggggtaggg gtcattgcca gcatggcggt
    24361 ggatccggtc gccgatcccg accttgtgcg cgttgatgct cacgatatct tcagccacag
    24421 tacaaccaaa attggttttc gccgtagtac ttttttgcgc agttatatgt atgatttcat
    24481 tcagcgtttt gcaccgcatt taacgcgtga tgtcgttgat gcggctgtcg cattgcgctc
    24541 caatgaagaa attgaggtca tgtttaaaga tataaaactg ccggaaaaat aatttcttgc
    24601 gttattttcg gcacctttta tgtagcgaag gtgccggaat atattctctt ttgttactta
    24661 tttctatacc gtgcacaata tttttatccc tcatatttat agggtaaatt acctgtaaag
    24721 tagtgtctaa ttacccgcct cgcaaattcc agttttcaaa tatctatttc aggtcaaaag
    24781 attgaattat tcactggaga cgattcgtaa attcgctgga tttttgacta aagtttcttt
    24841 aggatttgtc tcatcgtatg attaattaca ccaatcattc aacgtcttgc tgataagtga
    24901 tggcgattat atgaggttag caatgccttc tggaaatcag gaaccccgcc gtgatcctga
    24961 attaaaacgt aaagcctggc tgacggtttt tcttggttct gcacttttct gggtggttgt
    25021 cgcactgctg atttggaaag tgtggggata actatggtcg gtcaggagca actggagtcg
    25081 tcaccattat gccagcatag tgacaatgaa ccagaaacga aacgggaatg ttccgtcgtt
    25141 attccagacg actggcaact aacatcgcag cagcaagcct ttatagaact gtttgctgaa
    25201 gatgatcagc cgaaacaata attatcatca ttcttattac ccatttttaa tgaattaaag
    25261 ggcttttaat acaccgcagc aataacagct tgagttatct caacacaaaa taataaccgt
    25321 taagggtgta gcctatgatc aacacaaata tgaaatattg gtcctggatg ggcgcgtttt
    25381 ctctgtcgat gctcttctgg gccgaactcc tctggatcat tactcactga tccttgaccc
    25441 cgctgcggcg gggttgtcat ttgctttgcc acaaggtttc tcctctttta tcaatttggg
    25501 ttgttatcaa atcgttacgc gatgtttgtg ttatctttaa tatttaccct gaagagaatc
    25561 agggcttcgc aaccctgtca ttaaggagga gctatgtcgt caaccctacg agaagccagt
    25621 aaggacacgt tgcaggccaa agataaaact taccactact acagcctgcc gcttgctgct
    25681 aaatcactgg gcgatatcac ccgtctaccc aagtcactca aagttttgct cgaaaacctg
    25741 ctgcgctggc aggatggtaa ctcagttacc gaagaggata tccacgcact ggcgggatgg
    25801 ctgaaaaatg cccatgctga ccgtgaaatt gcctaccgcc cggcaagggt gctgatgcag
    25861 gactttaccg gcgtacctgc cgttgttgat ctggcggcaa tgcgcgaagc ggttaaacgc
    25921 ctcggtggcg atactgcaaa ggttaacccg ctctcaccgg tcgacctggt cattgaccac
    25981 tcggtgaccg tcgatcgttt tggtgatgat gaggcatttg aagaaaacgt acgcctggaa
    26041 atggagcgca accacgaacg ttatgtgttc ctgaaatggg gaaagcaagc gttcagtcgg
    26101 tttagcgtcg tgccgccagg cacaggcatt tgccatcagg ttaacctcga atatctcggc
    26161 aaagcagtgt ggagcgaatt gcaggacggt gaatggattg cttatccgga tacactcgtt
    26221 ggtactgact cgcacaccac tatgatcaac ggccttggcg tgctggggtg gggcgtgggt
    26281 gggatcgaag cagaagccgc catgttaggc cagccggttt ccatgcttat cccggacgta
    26341 gtgggcttta aacttaccgg aaaattacgt gaaggtatta ccgccacaga cctggttctc
    26401 actgttaccc aaatgctgcg caaacatggc gtggtgggga aattcgtcga attttatggt
    26461 gatggtctgg attcactacc gttggcggat cgcgccacca ttgccaatat gtcgccagaa
    26521 tatggtgcca cctgtggctt cttcccaatc gatgcagtaa ccctcgatta catgcgttta
    26581 agcgggcgca gcgaagatca ggtcgagttg gtcgaaaaat atgccaaagc gcagggcatg
    26641 tggcgtaacc cgggcgatga accgattttt accagtatgt tagaactgga tatgaatgac
    26701 gttgaagcga gcctggcagg gccaaaacgc ccacaggatc gcgttgcact gcccgatgta
    26761 ccaaaagcat ttgccgccag taacgaactg gaagtgaatg ccacgcataa agatcgcctg
    26821 ccagtcgatt atgttatgaa cggacatcag tatcagttac ctgatggcgc tgtggtcatt
    26881 gctgcgataa cctcgtgcac caacacctct aacccaagtg tgctgatggc cgcaggcttg
    26941 ctggcgaaaa aagccgtaac tcttggcctc aagcggcaac cgtgggtcaa agcgtcgctg
    27001 gcaccgggtt cgaaagtcgt ttctgattat ctggcaaaag cgaaactgac accgtatctc
    27061 gacgaactgg ggtttaacct tgtgggatac ggttgtacca cctgtattgg taactctggg
    27121 ccgctgcccg atcctatcga aacggcaatc aaaaaaggcg atttaaccgt cggtgcggtg
    27181 ctgtccggca accgtaactt tgaaggccgt atccatccgc tggttaaaac taactggctg
    27241 gcctcgccgc cgctggtggt tgcctatgcg ctggcgggaa atatgaatat caacctggct
    27301 tctgagccta tcggccatga tcgcaaaggc gatccggttt atctgaaaga tatctggcca
    27361 tcggcacaag aaattgcccg tgcggtagaa caagtctcca cagaaatgtt ccgcaaagag
    27421 tacgcagaag tttttgaagg cacagcagag tggaaggaaa ttaacgtcac acgatccgat
    27481 acctacggtt ggcaggagga ctcaacctat attcgcttat cgcctttctt tgatgaaatg
    27541 caggcaacac cagcaccagt ggaagatatt cacggtgcgc ggatcctcgc aatgctgggg
    27601 gattcagtca ccactgacca tatctctccg gcgggcagta ttaagcccga cagcccagcg
    27661 ggtcgatatc tacaaggtcg gggtgttgag cgaaaagact ttaactccta cggttcgcgg
    27721 cgtggtaacc atgaagtgat gatgcgcggc accttcgcca atattcgcat ccgtaatgaa
    27781 atggtgcctg gcgttgaagg ggggatgacg cggcatttac ctgacagcga cgtagtctct
    27841 atttatgatg ctgcgatgcg ctataagcag gagcaaacgc cgctggcggt gattgccggg
    27901 aaagagtatg gatcaggctc aagccgtgac tgggcggcaa aaggacctcg tctgcttgga
    27961 attcgtgtgg tgattgccga atcgtttgaa cgaattcacc gttcgaattt aattggcatg
    28021 ggcatcctgc cgctggaatt tccgcaaggc gtgacgcgta aaacgttagg gctaaccggg
    28081 gaagagaaga ttgatattgg cgatctgcaa aatctacaac ctggcgcgac ggttccggtg
    28141 acgcttacgc gcgcggatgg tagccaggaa gtcgtaccct gccgttgtcg tatcgacacc
    28201 gcgacggagt tgacctacta ccagaacgac ggcattttgc attatgtcat ccgtaatatg
    28261 ttgaagtaac aagtatttgc ttgccggtta ttttgcttcc ggcaagcaaa tgaattacac
    28321 aatgcaagag ggttatttgt tcagcaaatg gcccattttc tcggctttgg tatcgagata
    28381 atgttcgtta ttggggttac gacctacaat caatggtacg cgttcaacaa tattaatccc
    28441 tgcttcggtc agaatttcga cttttttcgg gttattggtt aacaagcgga cttcattgac
    28501 gccaaggagt ttgaacatat cagcgcaaag agtgaagtcg cgctcatcag cggcgaagcc
    28561 taactggtgg ttagcctcta cggtatcgta accttgatcc tgcagtgcgt aagcgcggat
    28621 tttattcagc agaccaatgt tacgaccttc ctgacggtga tacagcaaaa taccacggcc
    28681 ttcctcggca atttgcgtca atgccgcttc gagctggaag ccacaatcgc agcgcaagct
    28741 gaacagggcg tcaccggtca gacattcgga atggacgcgc gcaagtaccg gggtatgccc
    28801 ggaaatatcg gcatagacta gcgcgacatg atcgtgtccg gttgccagtt cttcaaatcc
    28861 caccatcagg aaatcgcccc atggggttgg cagtttggct tctgccacac gtttaagctg
    28921 catgaaattc tccagataat gctggttctg tattggctta ttttgccata acgagaaggg
    28981 gttcacctaa tcacgacgcg tcgatcgttc acggaatggc acaaatctgt caatttttct
    29041 tggaactggc attttcagtt atgattgtgg gacttatcaa aaaggagagg ccatgcgttc
    29101 gattgcccga cgtaccgcag tgggagctgc actattgttt gtcatgccag tagccgtatg
    29161 gatttctggc tggcgttggc aacctggaga acaaagttgg cgactaaaag cggctttttg
    29221 ggttactgaa actgtcaccc agccctgggg cgtcattaca catttgattt tgttcggctg
    29281 gtttctctgg tgtctgcgtt ttcgcattaa ggctgccttt gtattatttt ccattctggc
    29341 ggcggcaatc cttgttggac aaggcgttaa atcctggatc aaagacaaag tacaggaacc
    29401 acgacctttt gttatctggt tggaaaaaac acatcatatt ccggttgatg agttctacac
    29461 tttaaagcga gcagaacgcg gaaatctagt aaaagaacag ttggctgaag agaaaaatat
    29521 tccacaatat ttgcgttcac actggcagaa agagacgggg tttgcctttc cttccggtca
    29581 cacgatgttt gctgccagtt gggcactgct ggccgttggt ttgctgtggc cgcgtcggcg
    29641 aacgttaacc attgctatct tgttggtctg ggcaacggga gtcatgggaa gccgcctgct
    29701 actcgggatg cattggccac gcgatctggt ggtagctacg ttgatttcgt ggacgctggt
    29761 ggcggtggca acgtggcttg cgcaacgaat ttgtgggcca ttaacaccac ctgcggaaga
    29821 aaatcgcgaa attgcacaac gagaacaaga aagttaacgc tggttgattt tccgaattta
    29881 ggccttaaat catcaacaat gcgcgtggat gccatttcgc agacggcgcg aaaatggtac
    29941 tttaaagggc tattgcggta agttgaccat aatttattcg ctctaaccac ataacgggaa
    30001 gtaatgtgaa atatttactc attttcttac tggtgttagc gatcttcgtg atttcggtca
    30061 cgttgggtgc gcagaacgat caacaggtga cgtttaatta tctgttagcg caaggggagt
    30121 accgtatttc cacattgctg gcggtattgt ttgctgcggg gtttgctatc ggttggttga
    30181 tttgtggcct gttctggctg cgagttcgtg tttccctggc gcgcgctgaa cgtaaaataa
    30241 agcgactgga aaaccagctt tcacccgcga ctgacgtggc tgtagtgccg cactcgtcag
    30301 cggcgaagga ataactttct atgctggagt tgttgtttct gcttttgcct gtagccgctg
    30361 cctatggctg gtatatgggc cgcagaagtg cgcaacaaaa caagcaagat gaagccaacc
    30421 gcttgtcgcg tgattacgta gcgggggtta acttcctgct tagtaatcaa caggataaag
    30481 cggtagacct gtttctcgat atgcttaaag aggatacagg caccgttgaa gcccacctta
    30541 cgctcggaaa cctgttccgt tcgcgtggcg aagttgatcg cgctattcgc atccatcaga
    30601 ccctaatgga aagcgcctcg ctgacctatg aacagcgtct gttggcgatt caacaactgg
    30661 ggcgtgatta catggccgcc gggttatacg accgcgcgga agacatgttc aatcagctga
    30721 ccgatgaaac tgacttccgc attggcgcgc tgcaacagtt gctacaaatc taccaggcta
    30781 ccagtgagtg gcagaaagca attgatgttg ccgaacgcct ggtgaagctg ggtaaagata
    30841 aacagcgcgt cgaaattgcc catttctact gtgagttagc cctgcagcat atggccagcg
    30901 acgatctcga tcgtgccatg accttgctaa aaaaaggggc ggcggcagat aaaaacagcg
    30961 cccgcgtatc catcatgatg ggacgcgtgt ttatggcgaa aggagaatac gccaaagccg
    31021 tcgaaagttt gcaacgcgtc atatcccagg acagagaact ggtcagcgaa acgctggaaa
    31081 tgttgcaaac ctgctatcag cagttgggta aaactgccga atgggcagaa ttcctgcaac
    31141 gcgcggtgga agagaacacc ggtgccgatg ctgaattgat gcttgctgat atcatcgaag
    31201 cgcgcgacgg tagtgaggct gcacaggtct atattacacg ccagcttcag cgtcatccga
    31261 ccatgcgtgt attccataag ctaatggatt accacttaaa tgaagcggaa gaagggcgcg
    31321 ccaaagagag tctgatggtg ctgcgtgaca tggttggcga gaaggtgcgt agtaagcctc
    31381 gttatagctg ccagaaatgt ggttttaccg catacaccct ctactggcat tgtccgtctt
    31441 gtcgtgcctg gtcaactatt aaaccgattc gcggtcttga tggcctgtaa tttttaaaaa
    31501 aatccgactt tagttacaac atactagtta ttgatgtccc attgtgctcc ggcaacgacg
    31561 gagcagaaaa agcctgctag gggaacaatc gcaactgtta attttttatt tccacgggta
    31621 gaatgctcgc cgtttacctg tttcgcgcca cttccggtgc ccatcatcaa gaaggtctgg
    31681 tcatgacgtt aactgcttca tcttcttccc gcgctgttac gaattctcct gtggttgttg
    31741 cccttgatta tcataattgt gatgacgcgc tgtcctttgt cgacaagatc gacccacgcg
    31801 attgtcgtct gaaggtcggt aaagagatgt ttacattgtt tgggccacag tttgtgcgcg
    31861 aacttcaaca gcgtggtttt gatatctttc ttgacctgaa attccacgat atccccaaca
    31921 ctgcagcgca cgctgtcgct gctgcagctg acttaggcgt gtggatggtg aatgctcatg
    31981 cctccggtgg ggcgcgtatg atgaccgcag cgcgtgaggc actggttccg tttggcaaag
    32041 atgcaccgct tttgattgct gtgacagtgt tgaccagcat ggaagccagc gacctggccg
    32101 atcttggcgt gacactgtca cctgcagatt atgcagaacg tctggcagca ctgacgcaaa
    32161 aatgtggcct tgatggtgtg gtgtgttctg ctcaggaagc tgtgcgcttt aaacaggtat
    32221 tcggtcagga gttcaaactg gttacaccgg gcatccgtcc gcaggggagt gaagctggtg
    32281 accagcgccg cattatgacg ccagaacagg cgttggcggc tggtgttgat tatatggtga
    32341 ttggtcgccc ggtaacgcaa tcggtagatc cagcgcagac actgaaagcg atcaacgcct
    32401 ctttacagcg gagtgcatga tgagtgattc caacagccgt ctggtctact caacggaaac
    32461 cgggcgtatt gatgagccca aagcggcccc agtacgccct aaaggcgatg gagcggtgcg
    32521 tattcagcgt cagaccagtg gacgtaaagg taagggcgtt tgcctgatta ccggtatcga
    32581 tctcgatgat gccgaactga caaaacttgc agcggaactg aagaaaaaat gcggctgcgg
    32641 cggagcagtt aaagatggag ttattgaaat ccagggcgat aagcgtgatt tattaaaatc
    32701 actacttgaa gcgaaaggga tgaaggtaaa actcgcaggc ggttaacata aaaagccacg
    32761 gatatatccg tggctttcga atattttact gtgtgtatta ttcagttatt tcgtactgaa
    32821 cagaatcaag cgtattataa ttgcgagtcg ataatcgaca tttatttacg attatttacc
    32881 gacctggtga ctaataacac caccgacagc tgcaccacct aatgtaccca acgtactgcc
    32941 atcggtcagt actgcaccgc ctaatgcccc tgcacccgcg ccgattgcgg tgttgcggtc
    33001 ccgtttagac cagttagaac aggcactcag agacattgcc aaagtaattg ccagaacagc
    33061 cgcggtcatt tttttgctcg ttacaaacat aatactctct cctgaattta tgattcacgg
    33121 aagtaagctc tctataacta taatagctaa gaataagcct ggtgaaatta ttccgtgaaa
    33181 tctgctcgcg cgcagggtta tatcacg
//
LOCUS       DAJTXY010000030        33150 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTXY010000030 DAJTXY010000000
VERSION     DAJTXY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.030"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO566_001555"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO566_001555"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6604882.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO566_001556"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO566_001556"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6604883.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO566_001557"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO566_001557"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6604884.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO566_001558"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO566_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6604885.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO566_001559"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO566_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6604886.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO566_001560"
                     /pseudo
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO566_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO566_001561"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO566_001561"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6604887.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO566_001562"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO566_001562"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6604888.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO566_001563"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO566_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6604889.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO566_001564"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO566_001564"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6604890.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO566_001565"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO566_001565"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6604891.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO566_001566"
     CDS             8212..9075
                     /locus_tag="OO566_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6604892.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO566_001567"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO566_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6604893.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO566_001568"
     CDS             10411..11028
                     /locus_tag="OO566_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6604894.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12542)
                     /gene="ligB"
                     /locus_tag="OO566_001569"
     CDS             complement(11025..12542)
                     /gene="ligB"
                     /locus_tag="OO566_001569"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6604895.1"
                     /translation="MEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTG
                     VRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQK
                     VSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTL
                     NSLAVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLP
                     FVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVA
                     SLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLGGQGIPRIDDVVWRGAERTK
                     PTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEH
                     IFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASD
                     ERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(12564..12821)
                     /locus_tag="OO566_001570"
     CDS             complement(12564..12821)
                     /locus_tag="OO566_001570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604896.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            12961..13584
                     /gene="gmk"
                     /locus_tag="OO566_001571"
     CDS             12961..13584
                     /gene="gmk"
                     /locus_tag="OO566_001571"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6604897.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13639..13914
                     /gene="rpoZ"
                     /locus_tag="OO566_001572"
     CDS             13639..13914
                     /gene="rpoZ"
                     /locus_tag="OO566_001572"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6604898.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13933..16041
                     /gene="spoT"
                     /locus_tag="OO566_001573"
     CDS             13933..16041
                     /gene="spoT"
                     /locus_tag="OO566_001573"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6604899.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16048..16737
                     /gene="trmH"
                     /locus_tag="OO566_001574"
     CDS             16048..16737
                     /gene="trmH"
                     /locus_tag="OO566_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6604900.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16743..18824
                     /gene="recG"
                     /locus_tag="OO566_001575"
     CDS             16743..18824
                     /gene="recG"
                     /locus_tag="OO566_001575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6604901.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18858..20063)
                     /gene="gltS"
                     /locus_tag="OO566_001576"
     CDS             complement(18858..20063)
                     /gene="gltS"
                     /locus_tag="OO566_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6604902.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20343..21734
                     /gene="xanP"
                     /locus_tag="OO566_001577"
     CDS             20343..21734
                     /gene="xanP"
                     /locus_tag="OO566_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6604903.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21855..23564
                     /locus_tag="OO566_001578"
     CDS             21855..23564
                     /locus_tag="OO566_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6604904.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23617..25935)
                     /gene="yicI"
                     /locus_tag="OO566_001579"
     CDS             complement(23617..25935)
                     /gene="yicI"
                     /locus_tag="OO566_001579"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6604905.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25945..27328)
                     /locus_tag="OO566_001580"
                     /pseudo
     CDS             complement(25945..27328)
                     /locus_tag="OO566_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27621..27715
                     /locus_tag="OO566_001581"
     tRNA            27621..27715
                     /locus_tag="OO566_001581"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27655..27657,aa:Sec,seq:tca)
     gene            28015..29199
                     /locus_tag="OO566_001582"
     CDS             28015..29199
                     /locus_tag="OO566_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6604906.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSALIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29611..30654
                     /locus_tag="OO566_001583"
     CDS             29611..30654
                     /locus_tag="OO566_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6604907.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSRIQYQ"
     gene            31169..31597
                     /locus_tag="OO566_001584"
                     /pseudo
     CDS             31169..31597
                     /locus_tag="OO566_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31627..32778)
                     /locus_tag="OO566_001585"
                     /pseudo
     CDS             complement(31627..32778)
                     /locus_tag="OO566_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         8290 a         8729 c         8201 g         7930 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cagaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagattc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagataca
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttagcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagcagc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttgggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatggttt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgcagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatgcctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggatcgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccg agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgagttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccgcc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactattgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcacta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttccag tagtcatcgt cccactgttt tatttgctgt tgcaggcggg
    12601 aaatttcttc ctgtgctctg gctggcgacc aggccggaca gaccgcccac acagatgatt
    12661 gccagcacaa gatacttatt aatatcgcca tccatacttt catcttcacc tccgctgtag
    12721 tcaggctggc agatatacaa cgtggtgaag gcaaagccga gtggcaaaaa cggagtctgc
    12781 gaggacgctt cctgagaatc gtctttattg cagtaaatca caggcaaatg cggaaacagc
    12841 tacgcaaaac gcaacaactt tgcgcaaaaa gtgtgagcaa gggctacgtc acatggccgc
    12901 gccgtgtata ataagctcgt atgtaggctt tatttcgcta atcacatacg aaagatactc
    12961 atggctcaag gcacgcttta tattgtttct gcccccagtg gcgcgggtaa atccagcctg
    13021 attcaggctt tattaaaaac ccaaccgttg tatgacaccc aggtttctgt ttcacacacc
    13081 acacgccaac cgcgtcctgg tgaagtccac ggtgaacatt atttctttgt taatcatgat
    13141 gaatttaaag aaatgattag cagagatgcg ttcctcgaac acgcagaagt ttttggtaat
    13201 tactatggca cttcgcgtga ggccattgag caagtactgg cgaccggtgt cgatgttttt
    13261 ctcgatatcg actggcaggg cgcgcagcaa attcgccaga agatgccgca cgcgcggagt
    13321 atctttattt taccgccgtc caaaattgaa ctggaccgcc gtctacgcgg tcgcggtcag
    13381 gacagcgaag aggtcattgc aaagcgtatg gcgcaagctg ttgcagaaat gagccattac
    13441 gccgaatatg attatctgat tgtgaatgat gacttcgata ccgcgttgac cgatttgaag
    13501 accattattc gcgccgaacg tctgcgcatg agccgccaaa agcagcgtca tgacgcttta
    13561 atcagcaaat tgttggcaga ctgaacctga tttcagtatc atgcccagtc atttcttcac
    13621 ctgtggagct ttttaagtat ggcacgcgta actgttcagg acgctgtaga gaaaattggt
    13681 aaccgttttg acctggtact ggtcgccgcg cgtcgcgctc gtcagatgca ggtaggcgga
    13741 aaggatccgc tggtaccgga agaaaacgat aaaaccactg taatcgcgct gcgcgaaatc
    13801 gaagaaggtc tgatcaacaa ccagatcctc gacgttcgcg aacgccagga acagcaagag
    13861 caggaagccg ctgaattaca agccgttacc gctattgctg aaggtcgtcg ttaatcacaa
    13921 agcgggtcgc ccttgtatct gtttgaaagc ctgaatcaac tgattcaaac ctacctgccg
    13981 gaagaccaaa tcaagcgtct gcggcaggcg tatctcgttg cacgtgatgc tcacgagggg
    14041 caaacacgtt caagcggtga accctatatc acgcacccgg tagcggttgc ctgcattctg
    14101 gccgagatga aactcgacta tgaaacgctg atggcggcgc tgctgcatga cgtgattgaa
    14161 gatactcccg ccacctacca ggatatggaa cagctttttg gtaaaagcgt cgccgagctg
    14221 gtagaggggg tgtcgaaact tgataaactc aagttccgcg ataagaaaga ggcgcaggcc
    14281 gaaaactttc gcaagatgat tatggcgatg gtgcaggata tccgcgtcat cctcatcaaa
    14341 cttgccgacc gtacccacaa catgcgcacg ctgggctcac ttcgcccgga caaacgtcgc
    14401 cgcatcgccc gtgaaactct cgaaatttac agcccgctgg cgcaccgttt aggtatccac
    14461 cacattaaaa ccgaactcga agagctgggt tttgaggcgc tgtatcccaa ccgttatcgc
    14521 gtaatcaaag aagtggtgaa agccgcgcgc ggcaaccgta aagagatgat ccaaaaaatc
    14581 ctctcggaaa tcgaagggcg tttgcaggaa gcgggaattc cctgccgcgt cagtggtcgc
    14641 gagaagcatc tttattcgat ttactgcaaa atggtgctca aagagcagcg ttttcactca
    14701 atcatggaca tctacgcttt ccgcgtgatc gtcaatgatt ccgacacctg ttatcgcgtg
    14761 ctcgggcaaa tgcatagcct gtacaagccg cgtccgggtc gcgtgaaaga ctacatcgcc
    14821 attccaaaag cgaacggcta tcagtctttg cacacctcga tgatcggccc gcacggtgtg
    14881 ccggttgagg tccagatccg taccgaagat atggatcaga tggcggagat gggtgttgcc
    14941 gcgcactggg cttataaaga gcacggcgaa accagcacca ccgcgcaaat ccgcgcccag
    15001 cgctggatgc aaagcctgct ggagctgcaa cagagcgccg gtagttcgtt tgaatttatc
    15061 gagagcgtta aatccgatct cttcccggat gagatttacg ttttcacacc ggaagggcga
    15121 attgtcgagc tgcctgccgg tgcaacgccc gtcgacttcg cttatgcagt gcataccgat
    15181 atcggtcatg cctgcgtggg cgcacgcgtt gaccgccagc cttacccgct gtcgcagccg
    15241 cttaccagcg gtcaaaccgt tgaaatcatt accgcaccgg gcgctcgccc gaatgccgcg
    15301 tggctgaact ttgtcgttag ctcaaaagcg cgcgccaaaa ttcgtcagtt gctgaaaaac
    15361 ctcaagcgtg atgattctgt aagcctgggc cgtcgtctgc tcaaccatgc tttgggtggt
    15421 agccgtaagc tgaatgaaat cccgcaggaa aatattcagc gcgagctgga tcgcatgaag
    15481 ctggcaacgc ttgacgatct gctggcagaa atcggacttg gtaacgcaat gagcgtggtg
    15541 gtcgcgaaaa atctgcaaca tggggacgcc tccattccac cggcaaccca aagccacgga
    15601 catctgccca ttaaaggtgc cgatggcgtg ctgatcacct ttgcgaaatg ctgccgccct
    15661 attcctggcg acccgattat cgcccacgtc agccccggta aaggtctggt gatccaccat
    15721 gaatcctgcc gtaatatccg tggctaccag aaagagccag agaagtttat ggctgtggaa
    15781 tgggataaag agacggcgca ggagttcatc accgaaatca aggtggagat gttcaatcat
    15841 cagggcgcgc tggcaaacct gacggcggca attaacacca cgacttcgaa tattcaaagt
    15901 ttgaatacgg aagagaaaga tggtcgcgtc tacagcgcct ttattcgtct gaccgctcgt
    15961 gaccgtgtgc atctggcgaa tatcatgcgc aaaatccgcg tgatgccaga cgtgattaaa
    16021 gtcacccgaa accgaaatta atgttttatg aacccaacac gttatgcacg catctgcgaa
    16081 atgctcgcca ggcggcagcc tgatctgacc gtctgcatgg agcaggtcca caaacctcat
    16141 aacgtttctg cgattattcg taccgcagat gccgttggcg tacatgaagt tcacgccgtc
    16201 tggcctggta gccgcatgcg caccatggct tcggcagcgg cgggtagtaa cagctgggta
    16261 caggtgaaaa cacaccgcac cattggcgat gccgtcgctc atctcaaagg ccagggcatg
    16321 cagattctgg caacccatct ttctgataat gctgtcgatt tccgcgaaat tgattacact
    16381 cgcccgacct gcattttgat gggacaggag aaaacgggca tcacgcagga agcattggcc
    16441 ctggcggatc aggacatcat cattccgatg atcggcatgg tgcagtcgct gaatgtttcc
    16501 gttgcctcag ccctcattct ttacgaagcc cagcgtcagc ggcaaaatgc aggcatgtac
    16561 ctgcgtgaaa acagcatgtt gccggaagca gagcaacaac gcctgttgtt tgaaggcggc
    16621 tatccggtgc tggcgaaagt cgcaaaacgc aaaggcctgc cttatcccca cgtcaatcag
    16681 caaggcgaga tcgaagctga tgccgactgg tgggctacta tgcaggctgc agggtaagtg
    16741 ccatgaaagg tcgcctgtta gatgctgtcc cactcagttc cctaacgggc gttggcgcag
    16801 cacttagtaa caaactggcg aaaatcaacc tgcataccgt gcaggatcta ctcttacacc
    16861 ttcccctgcg ctacgaagat cgcacccatc tctaccccat cggagaacta ctgccgggcg
    16921 tttatgccac ggtggaaggc gaagtgctga actgcaatat ctccttcggc ggtcggcgga
    16981 tgatgacctg ccagatcagc gacggttccg gcatcctcac catgcgcttt ttcaatttca
    17041 gcgcggcaat gaaaaatagc ctggcgacgg gccgccgtgt actggcttat ggcgaagcaa
    17101 agcgcggtaa atatggtgcg gagatgatcc acccggaata ccgcgtgcag ggcgatctca
    17161 gcacgccaga attacaggaa acgctcacgc cggtttatcc aacaacggaa ggcgtaaagc
    17221 aggccacgct gcgtaaatta accgaccagg cgctggatct gctcgacacc tgcgccattg
    17281 aagaactcct gccgccggaa ctgtcacaag gaatgatgac gctaccggaa gcgttgcgca
    17341 ctttgcaccg cccgccaccg acgctacagc ttagcgatct ggaaaccggg cagcatccgg
    17401 cgcaacgtcg tctgattctg gaagaactgc tggcgcacaa cctcagcatg ttagccttac
    17461 gtgccggagc acagcgtttt catgcccagc cgctgagcgc caatgacacg ctgaaaaata
    17521 aactcctcgc cgccttaccg ttcaagccaa cgggcgcaca ggcacgcgta gtggcggaga
    17581 tcgagcatga tatggcgctg gatgtgccga tgatgcgcct ggtacagggc gatgtaggtt
    17641 ccggtaaaac gctggtcgcc gccctcgccg cgctgcgtgc gattgcccac ggcaaacagg
    17701 tggcactgat ggcaccaacc gaattactcg ccgagcagca cgccaataac ttccgcaact
    17761 ggtttgcacc gctcggtatc gaagtgggct ggctcgccgg taagcagaaa ggcaaagcgc
    17821 gactgtctca gcaggaagcc atcgccagcg gtcaggtgca gatgattgtc ggtacacacg
    17881 ccatcttcca ggaacaggtg cagtttaacg gcctggcgct ggtgattatc gacgaacagc
    17941 atcgttttgg cgtgcatcag cgtctggcat tgtgggagaa aggccagcag cagggcttcc
    18001 atccgcatca gttgatcatg accgccacgc cgatcccccg cacgctggca atgacggcgt
    18061 atgccgatct cgatacctcg gtgatagatg agctgccgcc aggccgcacg ccagtgacta
    18121 cggtcgctat tcctgatacc cgccgtaccg acatcattga ccgcgtgcgc cacgcctgca
    18181 taactgaagg tcgtcaggcg tactgggttt gtacgttgat tgaagagtca gagttactgg
    18241 aagcgcaggc ggcggaagct acctgggaag agttgaaact ggcgctacca gaattgaatg
    18301 ttggcctggt acacgggcgg atgaaaccag ccgagaaaca ggcggtgatg gcgtcgttta
    18361 aacaaggtga gctacatctg ctggttgcca ctaccgttat tgaagtcggt gttgatgtgc
    18421 ctaacgccag cctgatgatt atcgaaaacc ctgagcgtct gggtctggcg cagttacacc
    18481 agctgcgtgg gcgcgtaggt cgtggcgcgg tggcttctca ctgcgtgctg ctctacaaaa
    18541 cgccgctttc taaaacggcg caaattcgcc tgcaagtgct gcgcgacagt aacgacggtt
    18601 ttgtgattgc gcaaaaagat ctggagattc gcggccctgg cgaattgtta ggcacgcgtc
    18661 agacgggtaa tgctgaattt aaagtggcgg atttactgcg cgatcaggcg atgatcccgg
    18721 aagttcagcg cctggcgcga catattcacg aacgttaccc acaacaggca aaagccttga
    18781 tagaacgctg gatgccggag acagaacgtt actcgaatgc gtaaaaggcg gcggtgagaa
    18841 gaccgccgct tcatcggtta accggcaaaa atcggcaaca ttaaatacag cttaatcacc
    18901 agcgcattga cgatatcgat aaagaacgca ccgaccatcg gcaccaccaa aaacgccatg
    18961 tgcgacgggc caaagcgttc agtgatcgcc tgcatgttgg cgattgccgt tggcgttgca
    19021 ccgaggccaa aaccacagtg acccgcagcc agcactgccg catcgtagtt tttgcccatc
    19081 atgcgccagg taacgaagat ggcatacaac gccatgaaga tggtctgtac caccagaatc
    19141 gccagcatcg gcagtgccag cgaagccagc tcccacagtt tcagccccat caacgccatc
    19201 gccaggaaca acgacaagct tacgttaccc agcacggata ccgcacgctc aaagacgcgg
    19261 taaaagccca ttattgacag accgttgctc agaatcacgc caacaaacag tacacagacg
    19321 aaggtcggca gttcaaaagc agtgccagcc aaaagttgcg caacaatttt ccccaccgtc
    19381 aggcagatag caatcagcgc gatagtttca atcagcacca acgaggtgat catgcgtccc
    19441 acatccggct tttcaaacgc cgtcgggact tcctggtcat ccggaatacc gttcggcgtg
    19501 gtggagtgtt tcaccagata gcgcgccacc ggaccgccaa tcaagccgcc cagcaccaga
    19561 ccgaacgttg cacaggccat agccacttcc gtcgcattgg tgaagccata acgttcaatg
    19621 aacaatttac tccacgcagc gcccgtaccg tgaccgccgg aaagcgtaat agaaccggcc
    19681 aacagcccca tcagcggatc aagccctaac aggctagcca taccaatgcc aatggcattt
    19741 tgcatcacca acagaccaac aaccacaatc aagaagatgc caaccacacg cccaccggca
    19801 cgcaaactgg caatgttggc gttcaggcca atggtggcga agaaagccag cattaacgga
    19861 tcgcgcaggg acatatcaaa gttgacttcc cagcccatgc tttttttcag tactagtagc
    19921 gccagcgcca ccaacaaacc acccgcaaca ggttccggta tggtgtattt cttcaaaaag
    19981 gagacggaat ggaccaactt acgcccgagc agcaacgtca gcgttgcggc aacaagcgtt
    20041 gctaaagtat cgagatgaaa catagttact cctttgtatc cgcatcactc tttcatactc
    20101 gtcatacttc atcgcgcagt ggcaacttca tgaactatga cgggtatcga gttatgcttt
    20161 cctgggtcaa attctcagct gttaatgaac aaatggcatg gattttaagc agaaaaatac
    20221 caaaagttat ataaaaaagt gcatttatct catttaaata tttagatgag gatgtggcaa
    20281 acgtttgctt ttctctttcg gtcagctaaa atgcccgctt tgctaccacg ggattgtttt
    20341 caatgtctgt ttccaccctc gagtcagaaa atgcgcaacc ggttgcgcag actcaaaaca
    20401 gcgaactgat ttaccgtctt gaagatcgtc cgccgcttcc tcaaaccctg tttgccgcct
    20461 gtcagcatct gctggcgatg ttcgttgcgg tgatcacgcc agcgctatta atctgccagg
    20521 cgctgggttt accggcacaa gacacgcaac acattattag tatgtcgctg tttgcctccg
    20581 gtgtggcatc gattattcaa attaaggcct ggggtccggt tggctccggg ctgttgtcta
    20641 ttcagggcac cagcttcaac tttgttgccc cgctgattat gggcggtacc gcgctgaaaa
    20701 ccggtggtgc tgatgttcct accatgatgg cggctttgtt cggcacgttg atgctggcaa
    20761 gttgcaccga gatggtgatc tcccgcgttc tgcatctggc gcgccgcatt attacgccgc
    20821 tggtttctgg cgttgtggtg atgattatcg gcctgtcgct aattcaggtg gggctgacct
    20881 ccattggcgg cggttacgca gccatgagcg ataacacctt cggcgcaccg aaaaatctgc
    20941 tgctggcagg cgtggtttta gccttaatta tcctgcttaa ccgtcaacgt aacccttact
    21001 tacgcgtggc ctcactggta attgcgatgg cggccggata tgcgctggcg tggtttatgg
    21061 gcatgttgcc agaaagcaac gaaccgatga cgcaagaact gattatggtg ccaacgccgc
    21121 tctattacgg tcttggcatt gaatggagtc tgctgctgcc gctgatgctg gtctttatga
    21181 tcacttcgct ggaaaccatt ggcgatatca cggcgacctc tgacgtttcc gaacagccag
    21241 tgtccggtcc gctgtacatg aaacgcctga aaggcggcgt gctggcaaac ggcctgaact
    21301 cgtttgtttc ggcggtgttt aacaccttcc cgaactcctg cttcgggcaa aacaacggag
    21361 tgatccagtt gactggtgtt gccagccgct atgtcggttt tgtcgtcgcg ctgatgttga
    21421 tcgtgctggg tctgttcccg gcagtgagcg attttgtaca acacattcca gaaccggttc
    21481 tgggcggcgc aacgcttgta atgtttggca ccatcgccgc ctccggtgtg cgtatcgttt
    21541 ctcgtgagcc gctgaaccgt cgggcgattc tgattatcgc gttgtcgctg gcggttggtc
    21601 tgggcgtgtc tcagcagccg ctgattttgc agtttgcccc tgaatggctg aaaaacctgc
    21661 tctcctccgg gatcgccgcg ggcggtatta ctgccatcgt gctgaatctg attttcccac
    21721 cagaaaaaca gtaattcact ggcggcggta agataattac cgccgcagtc tcacctcttt
    21781 caccattccc gccttgagga ttgcgcgtaa atcgtgcata actcccttat gtgcatttca
    21841 cgggatggaa gaccatgaaa tttattggga agctgcttct ctacattctc atcgctctgt
    21901 tagtggtgat cgctggcctc tattttcttc tgcaaacccg ctggggagca gaacatatca
    21961 gcgcatgggt ttccgagaat agcgactatc atctggcctt cggggcgatg gatcaccgtt
    22021 tttccgcgcc atctcatatc gtgctggaga atgtcacgtt tggtcgtgat ggtcagcccg
    22081 cgcccctggt ggcaaaaagt gtcgacattg cgctaagcag tcgccaactg accgaaccac
    22141 gccatgtcga taccatcctg ctggaaaacg ggacgctgaa tctcaccgac cagaccgcgc
    22201 cgctaccgtt caaagccgat cgtctgcaac tgcgtgatat ggcgtttaat agcccgaata
    22261 gcgaatggaa actgagcgcg cagcgggtaa atggcggcgt ggttccgtgg tcaccagaag
    22321 ccggtaaagt gctgggtacg aaggcgcaga ttcagtttag tgccggatcg ctttcgctca
    22381 atgatgttcc tgccaccaat gtactgattg aaggcagtat tgataacgat cgcgttacgc
    22441 tgactaacct gggtgccgac atcgcccgcg ggacattaac cggaaacgcg cagcgtaacg
    22501 ccgacggcag ctggcaagtg gaaaatctgc gcatggcgga tatccgtcta caaagcgaaa
    22561 aatcgctaac cgacttcttt gcgccattac gctctgtccc gtcgttgcag attggtcgct
    22621 tggaagtcat cgatgctcgt ctgcaaggtc cggactgggc ggtgaccgac ctcgatctca
    22681 gcttgcgcaa catgaccttc agtaaagatg actggcagac acaggaaggc aaactgtcga
    22741 tgaacgccag cgagttcatt tatggttcgc tgcatttatt tgacccgatt ataaacgcgg
    22801 aattttcccc gcagggcgta gcgctgcgcc agttcaccag ccgctgggaa gggggtatgg
    22861 tcagaacgtc agggaactgg ctgcgtgacg gtaaaacgtt gatccttgat gatgcggcaa
    22921 ttgccgggct ggaatatacc ttgccgaaaa actggcaaca gttgtggatg gaaacgacac
    22981 ccggttggtt aaacagcctg caactgaaga gatttagcgc cagccgcaat ctgatcattg
    23041 atatcgaccc tgacttcccg tggcagctca ccgcgctcga tggttacggt gccaacctga
    23101 cgctggttac cgatcataaa tggggcgtct ggagtggctc ggcgaatctg aatgccgccg
    23161 ccgcgacatt caatcgtgtt gatgttcgtc gcccgtcgct ggcgctgacc gccaacagca
    23221 gcacggtgaa tatcagcgaa ctgagtgcat ttactgaaaa aggcattctg gaagccaccg
    23281 caagtgtttc acaaacgcca caacgtcaga cccatatcag cctgaatgga cgcggtgtgc
    23341 cggtgaatat tttgcaacaa tggggatggc ctgaattaca gttgactggc gacggcaata
    23401 ttcagcttac cgccagtgga gatattcagg ccaatgcccc gctgaaacct acggttagcg
    23461 ggcaattgca tgccgtgaac gccgcaaagc agcaagtgac tcaaaccatg aatgcgggcg
    23521 tcgtttccag cggtgaagtt acatcgacgg agccggtgcg gtaaacatca atctcgccgg
    23581 gtacgactca gacgtacccg gcattccgtc aatagattac aacgtaattg tcagcgcatt
    23641 cccttgaggc gtcaccacca gcccctgctc actttcagcc tgcgaaccgc cttgcagacc
    23701 atttactttc acaacattgc gcaagcacag cgtccagttc ttcgcctcgc ccgcaccagt
    23761 cacagtaatc gtgttgccag tacgtgctgc ttttaaagta aagatcaccg atccgtcagc
    23821 agcaggcact tcacagacgg cttcatgccc gtcttgcaga ttaaagaggt ggaatgccgt
    23881 gccttcgtgc cacacgtaat cgggacgttg atcgttgttg cccagcgcca gtagagtgtt
    23941 atcacgcaca taaacgggca gactcaggaa gccgtgctgc tgtttatgcc agcgactacc
    24001 gtcgagttca tcgttgtgcc acaggtgtgt ccagcgacct tccggcaggt agaactgcac
    24061 atcgcccgct tcagtgaaca ccggcgcaac catcacgttg tcgcctaaca tgtattgacg
    24121 gtcaaggtaa tcacaagccg gatcgtccgg gaactccatc atcatggccc gcatcatcgg
    24181 cgtaccccgc gcgttcgcac gcgcagcttc acgatacaga tacggcatca tgcggcattt
    24241 cagttgcgtg aagaagcgca ccacatcaca ggactcatca tcgtaggccc acggcacacg
    24301 ataagatttg ctaccgtgta aacggctatg gctggagagc aaaccaaacg cgcaccagcg
    24361 tttgtaaacg tgcgccggag cggtattttc aaagccgccg atatcgtggc tccagaagcc
    24421 aaaacctgaa aggccaatag acaaaccacc gcgcaggctt tccgccattg attcgtagtt
    24481 agcgtaacaa tcgccacccc agtgtaccgg gaatttctgc gcaccgacgg aggccgagcg
    24541 ggcaaacaag acagcttctt cctcaccaac ggtgtccttg agcacgttcc acaccagttc
    24601 gttgtagatg tacgcataat ggttatgcat tttctgcgga tcggaaccgt caaaccactg
    24661 aacatcagtt gggatacgtt cgccaaagtc ggtcttaaag caatcaacgc ccatcgcgac
    24721 cagacctttc agtttgtcgg cgtaccattt gcaggcatcc ggattggtaa agtcataaat
    24781 cgccagacct ggctgccatt tatcccactg ccatagcgaa ccgtccgggc gtttgagtaa
    24841 atagcctttc tcttgtaact ctttaaagac gggggatttt tgaccgatat aggggttaat
    24901 ccagacgcag attttcagcc ctttcgcttt caggcggcgg atcatccctt ccgggtcagg
    24961 gaaagtcagc gggtcccact caaaatcgca ccactggaag gctttcatcc agaaacagtc
    25021 aaagtggaaa acatgcagcg gcagattgcg ttccgccata ccatcgataa agctgtttac
    25081 cgtcgcttcg tcgtagttgg tggtaaatga agtggttagc cacaggccga aggaccacgg
    25141 gggcggcagc gccggacgac cagtaaagcg ggtataacga tcaagtaccg ctttcggcgt
    25201 cgggccgtcg ataacaaagt attcgagata ttcactctca acgctgaact gcactttgga
    25261 gactttctcc gatcccactt caaaagagac acactgggga tgattgacca gtaccccata
    25321 accacggtta gtcatgtaga acgggatatt tttatacgcc tgttcagtac ttgtgccgcc
    25381 gtcccggttc caggtctcta ccgtctggcc attgcgcacc agggcagtaa agcgctctcc
    25441 cagaccgtaa actgtttcgc caacgccaag atcaagccgc tcaaacatat aattgcgttg
    25501 attattcgtg tcctgcacgt agccattatt tttcacctga ctaccggtaa tacgttcgcc
    25561 gttgcgcaga aaatccagtg accagaactc acctttgctg acacgcgcgc ttaagttgcc
    25621 acttttaaac tcagcataac gttctgtgtt ttcgattgtg accttcacgt cctgcaaaat
    25681 attgagcgga taatgaggac cgttattcag cgccccctga aaatactcaa tccgcacacc
    25741 gacaatacct tcctgtgggg agaaaaagcg cagcgtaaat aaaggcgtat caagctgcca
    25801 ggtacgttca cgcacatcac ggggggcagc atagaccacc atttcattat cctgctgttc
    25861 aacctcgaat acctgaagcg ggtgaatcaa attgagccca ggttgaatca accagtttcc
    25921 atcgctaatt ttcattgccg ttccttagtt ctgcacttct tgagaggtga attgttgctg
    25981 gcaacggcgt ttaccctgag ccagctgttc cataacggtt ttcagattgt gcgtcgtgag
    26041 tgagtagtag cgtttagcga taatcgcgct cagcaaataa cagatcgccg gaacaatcgt
    26101 gaatagcgca ataatgatgc taatcgtggc gctgttctgc gctttttctg ccgcatcata
    26161 tccgccataa gccagcatcc agccgataag agcgccgccg aaggccaacc ccagtttgag
    26221 cacaaacagc gtgccagcaa aactgatccc ggtcaggcgt ttaccattgc accactcgcc
    26281 gtagtcgacg gtatcgaaca tcattaccca ctggataggt gtcaccagtt gatgcaacac
    26341 accaatcacg aagatgaaga caaacatagt gatgctggcc tgcatgggaa caaagaacat
    26401 cgcgaggcta atcactgcca gcagggcgtt cgtccaccag aagatagtga ctttacattt
    26461 ccagtcggtc agaggttttg ccagtgcgga accaatcagg ttacccacgc aataagtggt
    26521 gagaaaagcg acaaacactt ccggcgtgcc caaaatccat gtgacgtaat acatcatcgc
    26581 cccaccgcgt acgcacaccg ccaggatatt gaaaatggtt agtaaaccga caatccgcca
    26641 ctggtcgttt tgccagatat cacgtaaatc ttcccgcata gacgttgttg taggtggtgc
    26701 ttcaacgcgt tctttagtgg tgaagaaaca aaatgccagc atcattaatg ccaccacgga
    26761 aaggaccacg ataccgccct ggaaaccgag tggtttatta tcaccgccaa ttaaattaac
    26821 cagtggcatc atcagaacag tagaaagcat gcctcccgcg gtcgccagca caaaacgcca
    26881 ggattgcagc gagatacgct gagtcgggtc attggtgatt acaccaccca atgcgcagta
    26941 agggatattg acgacggtat aaagtaaggt aagttagggt gtaagtaatt gctgcataga
    27001 tcattttgcc gttcatactg agatctggcg tgctataggc cagtacacag acgatcccga
    27061 atggcagtgc gccaaacagt acccacggac gaaatttacc ccagcgagag cgcgttcgat
    27121 cggccaacaa ccccatgcaa ggatcggaaa tcgcatccag tgcacgagcg accaaaaaca
    27181 tggttccgac aaatccggca ggaatgccaa aaatatcggt ataaaagaac atcatatata
    27241 acattacgtt atcgaaaata atgtggctgg cggcgtctcc catgccataa ccaattttct
    27301 ctttaacgga caacacttca ctcttcatct gcttttcctt gatgctatag gggtgctgag
    27361 accgataacg cattttgtaa accatccctg tgagagcgaa tattccgata tctggttatt
    27421 aatttatgat tcttgtttta tgtgatcgtg gtagcgttaa ttccgctcat atatcattgt
    27481 aaaatatggg ttttatataa attataatgc tttcgtgata atacgctgcg tttattaggc
    27541 ggaaaaaact gatctggggg atgtagaaac tcaaggaagt agctataatg cgccccgcct
    27601 ccatgtagca atcgaggcgc ggaagatcgt cgtctccggt gaggcggctg gacttcaaat
    27661 ccagttgggg ccgccagcgg tcccgggcag gttcgactcc tgtgatcttc cgccagttta
    27721 cctcttctga actctcccaa tgtcaataaa tctcttctaa tcccttgtat atcagtattt
    27781 atttgtatcc tgaggtcaac ctacttcacc ccgtatcaag tttcttttgg gggtacttta
    27841 gggggtgtta tttcctattg aaatacaagc acccccataa taggaaaaat gtgatacata
    27901 acaacggctt ttaacaatat ctttgttata caaggggtgt attgggggta tcaggttagt
    27961 tactggcaga aatccccctc aggaacatga attcatcaaa agccggagga tgtcatggca
    28021 ttaaccgata tcaaagtgaa aaccgcaaaa cccaaagaca aaccctataa gcttgctgat
    28081 ggcggcggta tgtatctact gattaacact aatggttcta aatactggcg catgaagtac
    28141 cgctttgctg gtaaagaaaa gatgctttct attggcgtat accccgatgt aacgctagcc
    28201 gatgctcgcg agaaacgcag tgaagccagg aaactcctcg ctgctggagg cgatccaggg
    28261 gaggcaaaaa aggaagaaaa aatcgcgcag caaatgagtc tgaaaaatac gttcgaggct
    28321 attgctcgcg aatggcacca gttaaaagct gatcggtggt cattacgcta tcgtgatgaa
    28381 atcattgata cctttgaaaa agacattttt ccctacattg gcaagcgccc tattgccgaa
    28441 atcaaaccaa tggagttact ggaagcactg cgtaaaatgg aaaaacgtgg agctctggag
    28501 aaaatgcgca aggtccgcca gcgctgtggt gaggtgttcc gctatgcaat tgttactggc
    28561 cgggcagact ataacccagc ccccgatctt gccagtgctt tagctacacc gaaaaaagta
    28621 cattttccct ttcttactgc taatgaactt ccccacttcc tcaacgatct ggcgggttat
    28681 accggaagta taatcactaa gacagctact cagatcatta tgttaaccgg cgtacgaaca
    28741 caagaactgc gttttgcacg ctgggaagat atcgattttg agacaaaatt gtgggagatc
    28801 ccggcagaag taatgaagat gaagcgacct catattgtgc cgctctctga gcaggtcatt
    28861 atgctattta agcagcttga accaatctca aaacatcatc ccctagtctt tatcggtaga
    28921 aatgatcccc gcaaaccaat tagtaaggaa agcattaacc aagtaattga attacttgga
    28981 tataagggaa gactcacagg gcacggtttc agacacacca tgagtacaat tctgcatgaa
    29041 caaggcttta attccgcctt gattgaaatg cagttagctc atgtggataa aaattccatc
    29101 aggggtacct ataatcatgc cctgtatctc gatggccgcc gtgaaatgat gcaatggtac
    29161 gctgattaca ttgattcact ctccagccgg gaaagttaag tcagaaatac ttttccctga
    29221 tgttttcctc aattaagtat tttatcctct ggcctacaag gctggaggat ttttgttatt
    29281 atattatttt tttgcgtctt caaacttatt acaaaaacat aattaaaaac aaattgacat
    29341 ttttaaattg gcaattacaa aatacagcct tgcagttatt tcgatacctt tagcaaagga
    29401 aaagttgatt cagatataat ttagcatttc aatcatctga catcattata aaaaatgaga
    29461 ttcacataaa gataaaatta ttgttctcaa ggaaagcatg aaggggcttc gtaagcaata
    29521 ttcattcggt gccaagacag gacttgatac acagaatctc aacttttatg ctctcagact
    29581 atatcaccac atgttttaca ggctcagaaa atgaacgata gattatgctt tgaagtgcac
    29641 gataacaagg gttatttcgt atttccggat acctggtttg gcccattgct gggggagttt
    29701 gaagaagtgc ttgatgcata cgacgctgat gaaatcagcg agacgagtta catcaacaaa
    29761 ctccgacgcc ttgctcaacg ggagccggac tttattgata tacacgctca tctggcatat
    29821 gcatttctgg aacagaatgc cccgcgtaag gcactgaacg ccgctcttaa aggactggcg
    29881 gcaggtaatc gtatcatccc ggaaagtttc tgtggtgaaa taatctggat gcatcccgaa
    29941 aaccggcctt atctgcgcgc gctttatgca gcaattctcg caaatgtgca cttacagcgc
    30001 catcaggatg cggttatgct cactgataaa atactggcat acaatcctga agataatcag
    30061 ggggctcgct ggttgttggg ttcagagctg ttacgcaccg gcgatcatga gcgagccttc
    30121 agtgtactga aagagcatgc tgatgaattt tcaccttact ggtatgaact cggtctcctg
    30181 cattttctga acggggaaca tgttaaggcc gccacagctt ttcgccatgg ctttgccaca
    30241 aacacttaca tcgcagaaat gctgtgcgga aatctgcatc catttccact tgctgtgtgg
    30301 catgacttct ccggctctct ggatacggca gaagattact atgcaacata ctcaccatta
    30361 tggggacaat atccggaagc attgctgttt gtaaactggc tctataacca ttcctccgtg
    30421 ctgcatgaac gatcagaaat cattaaatgc gccgaaatgc tgattcagga agatgatttc
    30481 gaaatatgcg aaagtatttt aagacagcag gagcacctgt ggaaaagaat tgacaagaca
    30541 ctttctgagg aaattgtaca gaaatgcaga aatatgaacg gtgaatatat ctggccctgg
    30601 atattgccct tttccgcagc agggatgaaa cattcaagaa tccagtatca gtagatattg
    30661 cattagtgta actttaaaac aagtaataat ttattatcca ataataacac tgtgttcctt
    30721 ccccaggtga ttaccctggg gttattttct gaaagtttcc tttgcaaaag gatagcgttg
    30781 taaattactt taacgaatta ctccatgaac tcattaaagt tctttcgtaa tactttatcg
    30841 tcaaacacgt tatagctggc atcaaaaacc acagatttac cctgccagcg ggtaatcccc
    30901 tcctctggcg ttttcggttg aactggtatc cccgtccaac acaggcagcc attataaaca
    30961 gtaaaaaatt gtattcgctg ttatgaaatc aatagtcctg acgtactgtt gaatgtgctt
    31021 gtgttctgaa cctcgtacaa ccagtacatt tgatatcgaa ttataaacaa aacattgcat
    31081 ccaacttttt taatatggac aatagttgtc catacggtgg ttttatattc gataaatcaa
    31141 tggatttcat tgagtcacag gactggtaat ggatgaaaat gctttaaggt ttgcctcata
    31201 ctggcgcaac tcgcttgcag atgctgagtc aggaaagggc tgttttgaac ggaaagacgc
    31261 ccaaaatttc actcactggc atgggatagc ggcgggacgt cttgacgaag cgattgtcaa
    31321 taaatttttt aagggagaaa aagacgatgt cgaaactgtc aatgtcatct tacgcccaaa
    31381 agtttatttc cggttactgc agcatggtaa ggaccgttcc gcaggcacgc ctgatattgt
    31441 tacaccgata gtgacgccag ccttgctaag ccgtgagggt tttttatatc cgacgccagc
    31501 gacctccatt cccagagacc tgcttgaacc tttgccaaaa ggagcatttt cgattggtga
    31561 gattgggcag tatgacaaat acaagacgac ccatactgat cctacccacg taatgtggac
    31621 acggccctaa gcgagattat ggttttcaaa ttgttccggg ctgagaccgc cacaggcact
    31681 gtgacgacgc cagcgattgt aatcacactc gatataatta aacaccgttg cccgcattat
    31741 ttcccggctg ctaaagcgtt ccccgtggat acattccacc ttcagcgaat gaaagaagct
    31801 ttccacacag gcattgtcat aacagtagcc tttcgcactc atactgccac gcaggttgtg
    31861 tcgcttcagc attgcctgat aatcccctga acagtattga ccaccacggt ccgtatgaac
    31921 aatgacgctt tccgggcgtc ttctccgcca caacgccatt tgcagtgcat cacaggccag
    31981 ttgtgctgtc attcgcggtg acatcgacca gccaatgacg gcgcgtgacc acaggtcgat
    32041 gactactgcg agatacaacc agccctcatc ggtacgcaag tacgtgatgt cacccgccca
    32101 cttctggttt cgggccactg gcgctgaagt cctgctccag cagattctcc aatacgggca
    32161 ggccatgtgc acggtagctg accgggctga acttccggct ggctttcgcc cgcagcccct
    32221 gacgacgcag gctggcggca atggttttaa tattgaactc cggcagttcg tcagcaaggc
    32281 ggggagcacc gtatcgctgc tttgcctcaa agaatgcctt atggacagcg gcatcgcagg
    32341 tgagccgaaa ctgttggcgc aggctcatct ggtgacgacg cctgagccag acataccagc
    32401 cgctgcgggc aacccgaagt acacgacaca tcgctttgat gctgaactct gcccgatgat
    32461 tttcgataaa gacatacttc atttcaggcg cttcgcgaag tatgtcgcgg ccttttggag
    32521 gatggacagt tcctcagcct gctccgccag ttgtcgttta aggcggacat tttcagcggc
    32581 cagttcgctt tcgcgctctg acgaactcat ttgttgctgc tgtttactgc gccaggcata
    32641 aagctgagat tcatacaggc tgagttcacg ggctgcggcg gccacaccga tgcgttcagc
    32701 gagtttcagg gcttcgttac gaaattcagg cgtatgttgt ttacggggct tcttgctgat
    32761 tgatactggt tttgtcatga gtcacctctg gttgagagtt tactcactta gtcctgtgtc
    32821 cactattggt gggtaagatc accctccatt aaaactaaaa cgggcgcttt ctgatatatc
    32881 atctgtaagc gcagtaatac cattagtagc acctctcggc ggcgcattga ttattgaata
    32941 ttataactgg aatgttatat ttcttgttat gatgctgttc agtattataa taatgttatt
    33001 atcaccaaag ataactcatg caaatgagaa agtaatatgt caaaacagtg catcctttgt
    33061 gaccaaagag tttatctgcg gcactatact tgtctcatta atactcagtt ctctattttg
    33121 ctacatatca atatcaccat caattttcat
//
LOCUS       DAJTXY010000031        33006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTXY010000031 DAJTXY010000000
VERSION     DAJTXY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.031"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            17..4549
                     /locus_tag="OO566_001586"
     CDS             17..4549
                     /locus_tag="OO566_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR6604908.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDVWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSS
                     SDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTP
                     DDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIA
                     DALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDKTANGGAGAYNAS
                     HDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEEN
                     GAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALG
                     SSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEA
                     HDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIG
                     LNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEF
                     SVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVT
                     NIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAE
                     EENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNIT
                     LGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQRMVEMDNKLSKTESKL
                     SGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGST
                     NSQGEYSAALGAGIQW"
     gene            4917..6572
                     /gene="lldP"
                     /locus_tag="OO566_001587"
     CDS             4917..6572
                     /gene="lldP"
                     /locus_tag="OO566_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6604909.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6572..7348
                     /gene="lldR"
                     /locus_tag="OO566_001588"
     CDS             6572..7348
                     /gene="lldR"
                     /locus_tag="OO566_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6604910.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7345..8535
                     /gene="lldD"
                     /locus_tag="OO566_001589"
     CDS             7345..8535
                     /gene="lldD"
                     /locus_tag="OO566_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6604911.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8731..9204
                     /gene="trmL"
                     /locus_tag="OO566_001590"
     CDS             8731..9204
                     /gene="trmL"
                     /locus_tag="OO566_001590"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6604912.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9257..10078)
                     /gene="cysE"
                     /locus_tag="OO566_001591"
     CDS             complement(9257..10078)
                     /gene="cysE"
                     /locus_tag="OO566_001591"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6604913.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10158..11177)
                     /gene="gpsA"
                     /locus_tag="OO566_001592"
     CDS             complement(10158..11177)
                     /gene="gpsA"
                     /locus_tag="OO566_001592"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6604914.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11177..11644)
                     /gene="secB"
                     /locus_tag="OO566_001593"
     CDS             complement(11177..11644)
                     /gene="secB"
                     /locus_tag="OO566_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6604915.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11707..11958)
                     /gene="grxC"
                     /locus_tag="OO566_001594"
     CDS             complement(11707..11958)
                     /gene="grxC"
                     /locus_tag="OO566_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6604916.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12100..12531)
                     /locus_tag="OO566_001595"
     CDS             complement(12100..12531)
                     /locus_tag="OO566_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6604917.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12776..14320
                     /gene="gpmM"
                     /locus_tag="OO566_001596"
                     /gene_synonym="pgmI"
     CDS             12776..14320
                     /gene="gpmM"
                     /locus_tag="OO566_001596"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6604918.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14330..15613
                     /gene="envC"
                     /locus_tag="OO566_001597"
     CDS             14330..15613
                     /gene="envC"
                     /locus_tag="OO566_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6604919.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15617..16576
                     /locus_tag="OO566_001598"
     CDS             15617..16576
                     /locus_tag="OO566_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6604920.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16563..17597)
                     /gene="waaH"
                     /locus_tag="OO566_001599"
     CDS             complement(16563..17597)
                     /gene="waaH"
                     /locus_tag="OO566_001599"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR6604921.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17836..18861)
                     /gene="tdh"
                     /locus_tag="OO566_001600"
     CDS             complement(17836..18861)
                     /gene="tdh"
                     /locus_tag="OO566_001600"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6604922.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18871..20067)
                     /gene="kbl"
                     /locus_tag="OO566_001601"
     CDS             complement(18871..20067)
                     /gene="kbl"
                     /locus_tag="OO566_001601"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6604923.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20342..21199)
                     /gene="yibB"
                     /locus_tag="OO566_001602"
     CDS             complement(20342..21199)
                     /gene="yibB"
                     /locus_tag="OO566_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6604924.1"
                     /translation="MKSSTTIITAYFDIGRGDWTTNKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKSYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21503..22435
                     /gene="rfaD"
                     /locus_tag="OO566_001603"
     CDS             21503..22435
                     /gene="rfaD"
                     /locus_tag="OO566_001603"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6604925.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22445..23491
                     /gene="rfaF"
                     /locus_tag="OO566_001604"
     CDS             22445..23491
                     /gene="rfaF"
                     /locus_tag="OO566_001604"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6604926.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23495..24487
                     /gene="rfaC"
                     /locus_tag="OO566_001605"
     CDS             23495..24487
                     /gene="rfaC"
                     /locus_tag="OO566_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6604927.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24484..25692
                     /gene="rfaL"
                     /locus_tag="OO566_001606"
     CDS             24484..25692
                     /gene="rfaL"
                     /locus_tag="OO566_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6604928.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25728..26870)
                     /locus_tag="OO566_001607"
     CDS             complement(25728..26870)
                     /locus_tag="OO566_001607"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6604929.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26879..27892)
                     /locus_tag="OO566_001608"
     CDS             complement(26879..27892)
                     /locus_tag="OO566_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR6604930.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27917..28624)
                     /gene="rfaY"
                     /locus_tag="OO566_001609"
     CDS             complement(27917..28624)
                     /gene="rfaY"
                     /locus_tag="OO566_001609"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6604931.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28650..29657)
                     /gene="waaO"
                     /locus_tag="OO566_001610"
                     /gene_synonym="rfaI"
     CDS             complement(28650..29657)
                     /gene="waaO"
                     /locus_tag="OO566_001610"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6604932.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29700..30497)
                     /gene="rfaP"
                     /locus_tag="OO566_001611"
     CDS             complement(29700..30497)
                     /gene="rfaP"
                     /locus_tag="OO566_001611"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6604933.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30490..31614)
                     /locus_tag="OO566_001612"
     CDS             complement(30490..31614)
                     /locus_tag="OO566_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6604934.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31611..32669)
                     /gene="rfaQ"
                     /locus_tag="OO566_001613"
     CDS             complement(31611..32669)
                     /gene="rfaQ"
                     /locus_tag="OO566_001613"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6604935.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8732 a         8070 c         7788 g         8416 t
ORIGIN      
        1 atagcgagca tcattaatga acaaaatatt taaagttatc tggaaccctg cgacagggaa
       61 ttatactgtt accagcgaaa cggcaaaaag ccgtggcaag aaatctgggc gcagtaagct
      121 gttaatttct gcgctggttg cgggtggaat gttgtcgtcg tttggggcat tggcgaatgc
      181 cgggaatgac aacggtcagg gtgttgatta cggtagtgga tcagctggcg acgtctgggt
      241 tgctataggc aaaggggcga aagcaaatac ttttatgaac accagtggtt ccagtactgc
      301 tgtgggttat gacgctatag ctgaaggcca atatagctct gccatcgggt caaaaaccca
      361 tgcgattggt ggtgcatcaa tggcctttgg ggttagtgca atatcagaag gcgatagaag
      421 tatagcactg ggtgcctctt cgtattcatt gggccaatac tcaatggccc tggggcgtag
      481 cagtgtagct agcggtactg acagcctcgc atttggcaga caatcacttg ccagcgcagc
      541 gaacgctatt gcgataggtg ctgagaccga agccgctgaa aatgcaactg ctattggcaa
      601 taatgcgaag gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa
      661 agtcaatact atcgcattag gaaatggcag ccaggctctg gcagataatg caatcgccat
      721 aggccagggc aacaaagctg atggcgtgga tgccatcgct ctgggtaatg gtagccagtc
      781 gagaggctta aacaccattg ccttaggcac agccagtaat gcaactggtg ataagagtct
      841 tgcgcttggt agtaatagca gtgccaacgg tattaactct gtcgcgctgg gcgcagattc
      901 cattgcggat ttagacaata ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt
      961 taatgtgaaa aatggcgcga tcaagtctga cagttacgat gccattaatg gttcacagct
     1021 ttatgccatt agcgactcgg tagcaaaaag gcttggagga ggggctgcag tagatgttga
     1081 tgacggtact gttacagcac caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg
     1141 ggctgcgctc gctgtacttg atgaaaacac cctgcaatgg gaccaaacca aaggcaaata
     1201 cagcgctgct catggtacta gtagcccaac tgccagcgta atcaccgatg ttgcggatgg
     1261 cacgatttca gcctccagta aggatgcggt taacggttcc caactgaaag ctaccaatga
     1321 cgatgtcgaa gccaacacca ccaatatcac caacctgacg gattccgttg gtgaccttca
     1381 ggctgatgcc ctgctctgga acgaaactaa aaaggcattc agtgcagctc acggccagga
     1441 taccaccagc aaaatcacca acgttaaaga tgccgacctg acggctgaca gcactgatgc
     1501 tgttaacggc tctcagctga aaaccaccaa cgatgctgtg gcgacgaata ccaccaatat
     1561 cgccaataac acttccaata ttgccactaa caccaccaac atctctaacc tgactgagac
     1621 ggtgactaat cttggtgagg atgcgctgaa atgggataag gacaatggtg tattcacggc
     1681 agctcatggc accgagacca ccagcaaaat aaccaacgtt aaagatggcg acctgacgac
     1741 tggcagcacc gatgccgtta acggctctca gctgaaaacc accaacgatg ccgtggcgac
     1801 gaataccacc aatatcgcca ctaacaccac caacatctct aatctgactg agacggtgac
     1861 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtcttca ctgcagctca
     1921 tggcaacaat accgccagca aaatcaccaa tatcctggac ggcacagtca ctgcaaccag
     1981 ttccgatgcc attaacggta gccagcttta tgacttaagc agcaatatcg ccacctactt
     2041 cggcggcaat gcttctgtga atactgacgg cgtgtttacc ggtccaacct acaaaatcgg
     2101 tgaaacaaat tattataacg tcggcgatgc tctgctttgg gatgccaccg caggtaaatt
     2161 cagtgccaaa cacggtacta atggtgacgc aagcgtgatc actgatgtcg cagatggtga
     2221 aatttcagac tccagttctg acgcagtaaa cggctcacaa ctccacggcg tgagcagtta
     2281 tgttgttgat gcgctggggg gtggtgccga agtcaatgca gacggcacca tcactgcgcc
     2341 gacgtacacc attgctaatg ctgattacga taatgtcggt gatgccctga atgctatcga
     2401 taccactcct gacgacgctc tgctctggga tgcggacgcc ggtgaaaatg gtgcatttag
     2461 cgccgctcac ggaaaagata aaactgccag tgtaatcact aacgtcgcta acggtgcaat
     2521 ctctgctgcc agcagcgacg cgattaacgg ctcacaactc tataccacca ataagtacat
     2581 cgctgatgcg ctgggtggtg acgcagaagt caacgctgac ggcaccatca ccgcaccgac
     2641 ttacaccatt gcgaacgccg agtacaacaa cgtcggtgac gccctggatg cgcttgatga
     2701 taacgccctg ctgtgggata agactgccaa tggcggtgct ggagcctaca atgccagcca
     2761 tgacggtaaa gccagcatca tcactaatgt cgctaatggc agtattagtg aggacagtac
     2821 cgatgcagtg aacggttctc agttgaatgc gacgaatatg atgattgagc agaacaccca
     2881 aattatcaat cagctcgctg gtaacaccga cgcaacctat atcgaagaaa atggcgctgg
     2941 tatcaactac gttcgtacta acgacaacga tttagccttt aacgatgcaa gcgcctctgg
     3001 tgtcggcgct acagctgtag gttataacgc tgtcgcgtct ggtgccagca gcgtagccat
     3061 tggtcagaac agcagcagca ccgttgatac cggtattgcg ctgggtagca gctccgtttc
     3121 cagccgtgtg attgccaaag gttctcgtga cactagcgta acggaaaatg gcttggtgat
     3181 tggttatgac accactgacg gcgaactgct aggtgcattg tcaattggtg atgacggtaa
     3241 ataccgccaa atcatcaacg tagctgatgg ttcagaagcc catgacgccg ttacggttcg
     3301 ccagttgcag aacgctattg gagcggtcgc cactacgcca accaagtact ttcacgccaa
     3361 ctcaacggca gaagactcac tggccgttgg tgaagactcg ctggcaatgg gtgcgaaaac
     3421 tgtcgttaat ggtaatgcag gtattggtat cggtttgaac actctggttc tggctgatgc
     3481 gatcaacggc attgctatcg gcagtaacgc acgcgcaaac catgcaaaca gtatcgcaat
     3541 gggtaacggt tctcagacca cccgtggtgc acagactggc tacaccgcct acaacatgga
     3601 cgcaccgcag aactctgtag gtgagttttc tgtcggtagt gaagacggtc aacgtcagat
     3661 cacaaacgtc gcagctggtt cagcggatac cgatgcggtt aacgtgggtc agttgaaagt
     3721 cactgatgag cgcgtagcgc aaaataccca gagcattact aacctgaaca atcaggtcac
     3781 taatctggat actcgcgtta ctaatatcga aaacggtatt ggcgacattg tcaccaccgg
     3841 tagcaccaag tacttcaaga ccaacaccga tggcgtagat gccaacgccc agggtaaaga
     3901 tagcgttgct attggttctg gttccattgc tgccgctgac aacagcgtcg cactgggtac
     3961 cggttccgtt gcagaggaag aaaatacaat ctctgtaggt tcttctacta accaacgccg
     4021 tatcaccaac gtagctgcca gtgttaatgc caccgatgcg gttaacgtgt cgcagctgaa
     4081 atcttctgaa gcaggcggag tgcgctacga caccaaagct gatggttcta tcgactatag
     4141 caatatcacc ctcggtggtg gcaacggcag tacgactcgt atcagcaacg tctccgctgg
     4201 cgtcaacaac aacgacgcgg tgaactacgc gcagttgaag caaagcgcgc aagaaacgaa
     4261 gcaatacacc gatcagcgga tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa
     4321 gttgagcggt ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttatacacc
     4381 gggtgccagc atggcttcta ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt
     4441 aggtgtatcg atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa
     4501 tagccagggt gaatactccg ccgcactcgg tgccggtatt cagtggtaat gatccattaa
     4561 gttagtgtga ctaagggcaa acgccaaatt gcctgatgcg ttacgcgcat caggcaagat
     4621 aaggcgctcc gggtcaacaa tctaagggca attctctgat gaggattgcc cttttcttta
     4681 ccagacatct ccccccacaa gaattggccc taccaattct tcgcttatct gacctctggt
     4741 tcacaattcc ccaattaaaa ctcacatcaa tattgataat acataacatt tagttaacca
     4801 ttcattgtca ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac
     4861 aaggagatga gcaacagact cattacacga tgtgcgtgga ctctaggaga cctgcaatga
     4921 atctctggca acaaaactac gatcccgccg ggaatatctg gctttccagt ctgatagcat
     4981 cgcttcccat cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg
     5041 ccgcctcatg gacggtagca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg
     5101 tcgctaacgc gctggcctcg gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt
     5161 ggatcattat tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca
     5221 tcattcgctc gtctattctt tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg
     5281 gtttctgttt cggcgctttc cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta
     5341 ccgccgcatt gctggtcggc ctgggtttta aaccgctgta cgccgccgga ctgtgcctga
     5401 ttgttaacac cgcgccagtg gcatttggcg cgatgggcat tccaatcctg gttgccggac
     5461 aagtaacagg catcgacagc tttgagattg gccagatggt ggggcgtcag ctaccgttta
     5521 tgaccattat cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaaag
     5581 agacgtggcc tgcggtcgtg gttgcaggcg gctcgtttgc catcgctcag taccttagct
     5641 ctaacttcat tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc
     5701 tgacgctgtt cctcaaacgc tggcaaccag ttcgcgtatt ccgcttcggt gacttggggg
     5761 cgtcacaggt tgatataacg ctggcccaca ccggttacac cgcgggtcag gtgttacgtg
     5821 cctggacacc gttcctgttc ctgaccgcta ccgtaacact gtggagcatc ccgccgttta
     5881 aagccctgtt cgcatcgggt ggtgcgctgt atgagtgggt gatcaacatt ccggtgccgt
     5941 acctcaataa actggttgcc cgtatgccac cagtggtcag cgaggctaca gcctatgccg
     6001 ccgtgtttaa gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc
     6061 tctcgattgt ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc
     6121 tgaaagaact agctctaccc atctactcca tcggcatggt gctggcgttc gcctttatct
     6181 cgaactattc cggactgtca tcaacactgg cactggcact ggcgcacacc ggtcatgcat
     6241 tcaccttctt ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct
     6301 catctaacgc cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg
     6361 atctgttgct ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc
     6421 aatctatcgc tatcgcctgt gcggcggtag gcctggtggg caaagagtct gatttgttcc
     6481 gctttactgt caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc
     6541 aggcttatgt cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga
     6601 cgaggttgcc gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa
     6661 gttgcccgct gagcgccaac tggcgatgca actcggcgta tcacgtaatt cactacgcga
     6721 ggcgctggca aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt
     6781 tattcgctgg cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct
     6841 gatggccgat gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc
     6901 cagcaccgca tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct
     6961 ttgctttgaa gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt
     7021 tcatctggcg attgccgaag cctcacataa catcgtgctg ctgcaaacca tgcgcggttt
     7081 cttcgatgtc ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc
     7141 ggttttttca caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga
     7201 tgccgacggg gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa
     7261 acgattcgat gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcataa
     7321 tgagcattcg agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca
     7381 gcgcaacgca ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa
     7441 tacacgctgc gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg
     7501 aaaaacatgt ccgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg
     7561 gtagcactgg caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca
     7621 gccaaagcgg cggacgcgca tggcattccg tttactctct cgacggtttc cgtttgcccg
     7681 attgaagaag tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc
     7741 gatcgcggct ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg
     7801 gttttcaccg tggatatgcc gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg
     7861 agcggcccaa atgcggcaat gcgccgctac ttgcaggcgg tgacgcatcc gcaatgggcg
     7921 tgggatgtgg gcctgaacgg tcgtccacat gatttaggta atatctcagc ttatctcggc
     7981 aaaccgaccg gactggaaga ttacatcggc tggctgggga ataacttcga tccgtccatc
     8041 tcatggaaag accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg
     8101 atcctcgatc cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg aattgtggtt
     8161 tctaaccacg gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct
     8221 attgcagatg cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg
     8281 cttgatgtcg tgcgtatgat tgcgctcggt gccgacaccg tactgctggg tcgtgctttc
     8341 ctgtatgcgc tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgaa
     8401 aaagagatga aagtggcgat gacgctgact ggcgcgaaat cgatcagtga aattacgcaa
     8461 gattcgctgg tgcaggggct gggtaaagag ttgcctacgg cactggctcc gatggcgaaa
     8521 gggaatgcgg catagtcgtt tgcgccccct caccctaacc ctctccctca gggagagggg
     8581 accgttcggc gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc
     8641 ctcgcccctc cggggagggc cggggtgagg ggaaaaggcc gcacggttca ccaattttct
     8701 gctatcctgc ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa
     8761 attccgccaa atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctacat
     8821 atcatcgaac cgatgggatt tgcctgggac gacaagcgcc tgcgccgcgc ggggctggac
     8881 tatcacgagt ttaccgccgt tacgcgtcat catgactatc gcgcgttcct cgaagcagaa
     8941 aatccccagc gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaagc
     9001 tatcaggatg gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt
     9061 cttgatgccc tgcccgctga acaaaaaatt cgcattccga tggtgccgga cagccgcagc
     9121 atgaatctgt ccaatgcggt gtcggtagtg gtgtatgaag cctggcggca gctgggatat
     9181 ccgggagcgt tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg
     9241 gcacgatcac aggacattag atcccatccc catactcaaa tgtatggtta ataccgttga
     9301 aatgctggtc catatccatt gatggcttat cgctgtctgg tttaccgaca atacgagccg
     9361 gaacgccagc ggcggttgta tgcggcggca ccggttgcag caccacggaa cctgcgccaa
     9421 tcttcgcgcc gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca
     9481 caccttcacg aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca
     9541 cagattgcag aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg
     9601 tggcgtggtc gagcataatg ccacggccaa tttttgctgc cgggtgaata tcgacctgga
     9661 acgtcacaga aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca
     9721 accagtgacc gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg
     9781 ttgagtattt atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag
     9841 aggcgatcat ttccgggtca gcggcgtagg cttcttcaac cacttcacgg atagcaatag
     9901 caggcataat tggcgatgac agcttgttcg ccagcatgta gctcagtgca ctgccaaggt
     9961 tttcgtgctt gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca
    10021 gcgttctggc ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg
    10081 cttactccac acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta
    10141 acaaaggttc cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg acctaataat
    10201 gtcaatgctg cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt
    10261 attggcattt caacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag
    10321 ccttccacca cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc
    10381 atcatgccaa aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc
    10441 agccccgcca tgcccataaa ggtggcagga tcggcaccca gcgccgcacc aagacgcgac
    10501 atttcagcca gcccacgggt gatcagcgcc gtacgcgcat tcgcaccaaa accgataccg
    10561 tcagacatcc ccgcgccaat ggcaatgacg tttttcaccg cgccgccaag ctgcacgcca
    10621 atgaaatccg ggttgctgta aacgcggaaa cttttgccgc agtgcagcag ctgctggaga
    10681 tcatcggcaa aggtctgatc ggtcgaggcc agcgaaatag ctgtcggtaa acctgccgcc
    10741 agttctttcg caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc
    10801 tcacgcgcca cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt ggtcgcccac
    10861 accagacgcg catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca
    10921 tggctgggta cgacgacgag aatattacga ctggctgcca gcgcagtggc gagatcgctt
    10981 tcaagatgga gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg
    11041 cgttcaagcg ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt
    11101 cttgccaggg taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa
    11161 gcattacgtt ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct
    11221 gcaaatagtt catgaacagc gcatcgaagt taaccggcgc aaggttcagt tgcgggaatg
    11281 taccgcggga taccatgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc
    11341 agtatgcgcc caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac
    11401 cgccctgctg aacttcacac aggaacgcgg tttcttcgcc caaggaggcc gttacggtaa
    11461 cacgcagtac cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa
    11521 gtttaacttc tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag
    11581 agatatcctt ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg
    11641 acatgtgtag aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt
    11701 cacacgttat ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc
    11761 acagccgcca atgtgctgtg cgtcaataaa aatctgggga accgtggtgc gaccgctgcg
    11821 tttgatcatc tcttcacgct tggcggcatt gccatcgatc ggcaactcct ggaaactcac
    11881 gcccttgctg ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata
    11941 gatttcaaca ttggccataa tttagctcct gtttttttac cctgaaattt catgttgcag
    12001 ggcggctgca aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat
    12061 tgcagctaac ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg
    12121 caagttttcg ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc
    12181 agctttcgtc agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat
    12241 aaccgatttg tctttgtgct tctcaagctc accaacattg ttggctttga tttcgctcgg
    12301 caacagattg atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc
    12361 cacaaccaca gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt
    12421 cactttcgag gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca
    12481 ggcgatactc agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa
    12541 caactcccaa cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa
    12601 atacagccag cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa
    12661 atctggcatc ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg
    12721 attcagctgt agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc
    12781 ggtttctaaa aaacctatgg tactggtgat tctggatggc tatggctatc gtgaagaaca
    12841 gcaggataac gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg
    12901 tccgcatacc ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg
    12961 taactccgaa gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac
    13021 tcgtctggac gttgaaatca aagatcgcgc tttctttgct aatccagtgc tggctggcgc
    13081 ggtacataaa gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgcagg
    13141 cggcgtacac agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg
    13201 cgcagaaaaa atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc
    13261 tgaatcctcg ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc
    13321 gtccatcatt ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa
    13381 agcctatgac ctgctgactc tggcgcaggg tgagttccag gccgatactg ccgttgctgg
    13441 tttgcaggca gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg
    13501 tgcagaaggt cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt
    13561 ccgtgctgac cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt
    13621 cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat
    13681 caaaactgcg gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc
    13741 gaaaaacgac aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt
    13801 cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc
    13861 accgaaagtg gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa
    13921 actggttgcg gccatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg
    13981 cgacatggta ggtcacaccg gggtgatgga agcggcggtt aaagcggttg aagcactgga
    14041 tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac
    14101 cgctgaccac ggtaacgctg aacagatgcg cgatccagca acgggtcagg cacacacggc
    14161 acacaccaac ctgccagttc cgctgattta cgttggtgat aagaacgtga aagcggttgc
    14221 aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc
    14281 gcaagagatg actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa
    14341 ggcgattaat accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat
    14401 ctacgccagc gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga
    14461 gcgtgaccaa ctcaaatcta ttcaggccga tatcgccgca aaagagcacg cggtacgcca
    14521 aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc
    14581 tgaagccacc cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat
    14641 tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag
    14701 cctcgccgca caactggatg ccgcattccg tcagggcgag cataccggta ttcagctaat
    14761 tctcagcggt gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa
    14821 ccaggcgcga caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcaccatgca
    14881 gcgtgctgaa ctggaagaga aacaaagcga gcaacaaacg ctgttatatg aacagcgcgc
    14941 ccaacaggcg aaactgactc aggttctgaa cgagcgtaaa aagacgctgg cggggctgga
    15001 atcttccatc cagcaaggtc agcagcagct aagtgaacta cgtgccaacg aatcccgtct
    15061 gcgtaacagc attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gagaagcgcg
    15121 tgaggcccag gcggttcgcg agcgccagaa agaagcgacg cgcaaaggca ccacctacaa
    15181 accgaccgaa agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg cgccgcgtgg
    15241 tcaggcattc tggccggttc gcgggccgac gctgcatcgc tatggcgagc agctacaggg
    15301 tgaattacgc tggaaaggga tggttatcgg tgcctctgaa ggtactgaag ttaaagcaat
    15361 tgccgacggt cgagtgattc tggctgactg gctgcaaggt tacggtctgg tggtggtggt
    15421 tgagcacggt aaaggcgaca tgagtcttta cggctataat cagagcgcac tggtgagcgt
    15481 tggttcgcag gttcgcgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg
    15541 tcggccttca ctctatttcg aaattcgccg ccagggtcag gcggtcaatc cacagccgtg
    15601 gttgggaaga taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt
    15661 ggcgctctcc tcccccgtac ttgctggcaa acttgccatc gtcattgatg attttgggta
    15721 tcgcccgcac aacgaaaacc aggtgctggc gatgccttcc gctatctccg tcgctgtatt
    15781 acccgattca ccgcacgcca gagagatggc gaccaaagcg cataacagcg gtcacgaagt
    15841 gttgattcat ctcccgatgg caccgttgag taaacagccg ctggagaaaa atacgctacg
    15901 cccggagatg agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc
    15961 ctatgccgtg gggatcaaca accacatggg cagcaagatg acctctaacc tgtttggtat
    16021 gcagaaagtg atgcaggcgc tggagcgtta caatctttac ttcctcgaca gcgtaaccat
    16081 cggtaatacc caggcgatgc gcgccgcgca aggtactggc gtgaaggtga tcaaacggaa
    16141 ggtgttcctc gacgattcgc agaacgaagc ggacatccgt gtgcaattta atcgcgcaat
    16201 tgatctggcg cgtcgcaacg gttcaaccat tgccattggg catcctcacc cttcaacggt
    16261 acgcgtgcta caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag
    16321 cagcttgctg aatgaaccgc aggttgatac ttctacaccg ccgaaaaacg ctgtgcctga
    16381 tgcaccgcgt aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc
    16441 tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat
    16501 cgtttacttc cagcatcagt ggcaaggctg gggcaaacag cacgaagcgg cgaagtttaa
    16561 cgctagcgca aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc
    16621 cattgccata agcggaaaga ccacagtaac gcctggtaac cgaccttcac gctgcgcaca
    16681 ttggtaatca ggcgcttata cataccggaa gtaaatatct cggcaatcat ccgttggcgg
    16741 gtaagaatat ccggctcttt gcgcaccgca tggcaaacac gcaatgcttc gtaagttatt
    16801 tgctgatgaa attccggata aatcgtaatt ttgtcggcat aatttcgatt taatttctcc
    16861 agcaggcggg taatcttaat atagtgacgt tgataattaa ggtttttatt cccttgtcta
    16921 tgcaaccgac tcactgacgt attatgcaga taatatttat ataatgattg ctcggtatat
    16981 cgcgcacgca gcgcgttaaa catgaattct gttgtccaga caatatcctg atgatgtaat
    17041 ccggcaataa atttaatgtt atttttaaca ataacatcac ggcgataaac ccccatccag
    17101 acaacgtgag tccaacggcg cgaagaaagc cccatccgca gccagtccgg gccggttaat
    17161 acaccggttg agcgaaggcg atcgctgggg atggattgcc aggtttctcc cgtttcacga
    17221 aaagaccagt cagcgttgca ctgcgccacg tcgaggtcgt cctctaacgc catggtcatc
    17281 agcgtttcgt acatggtggg atagacctca tcgtcagcat cgacaaaagc gacatatttg
    17341 cccgtcgcca cctcgatccc acgattacgc gctaccgatg cgccagcatt cgcctgatgc
    17401 aacaaacgaa cgtgcggata gttttctgcg taatgctttg ctatttcaac agaattatcc
    17461 gttgaaccat cgttaataat aatgatttcc agagcagtcc aggtttgcgt aattaaagat
    17521 tccatacaag tgcggaaatc atcgcccgca ttatataacg gaataataac actaagttta
    17581 ttggtgctgt tcatcataaa ttaccgacga cctctgaagt tatcctgacg ttttacaggc
    17641 tttgtattaa gaaactatta agcctgtgag atattatttt tacaggtgag agagtcactt
    17701 cagggtagag agataatgag aacagctata taaaagatca gcgcaattac gaactcttta
    17761 ttacactgac aggattttac ataattaaca gattatcctc agataaaagg gctggaatac
    17821 cagcccttgt tcgtgttaat cccagctcag aataactttc ccggactggc ccgaacgcat
    17881 agcgtcaaag cccttctgga aatcatcgat agagaaacga tgggtaatga tcggcgagag
    17941 atcgaggcca gactgaatca gcgccgccat cttgtaccaa gtttcaaaca tctcacgacc
    18001 gtaaatgcct ttaatgaaca agcctttaaa gatcactttg gtccagtcga tagacatatc
    18061 agacggtgga atacccagca tcgcaatacg accgccgtgg ttcatggtat cgagcatggt
    18121 acgaaacgct ggcggcgcgc cggacatttc cagaccgaca tcaaagcctt cggtcatgcc
    18181 cagttcagcc atcacatcat taaggttttc tttcgcgacg ttaaccgcac gggtgatacc
    18241 cattttgcgc gccagctcaa ggcggtattc gttaacatcg gtgatcacca cattgcgtgc
    18301 accaacgtgt ttcgccaccg ccgcagccat aatacctatc ggacctgcac cagaaaccag
    18361 cacatcttcg cccaccagat cgaacgacag cgcggtatgc acggcgttac cgaagggatc
    18421 aaaaatggaa gccaggtcgt cggagatatt gtcggggatt ttgaaagcgt tgaacgccgg
    18481 aatcaccaga tattcggcga agcagcccgg acggttaaca ccgacaccaa tggtgttgcg
    18541 gcacagatgg gtacgaccgc cacgacagtt gcgacagtga ccgcaggtga tatggccttc
    18601 gccagaaacg cgatcgccga ttttgaagcc tttcacttcc tgaccaatac ctaccacttc
    18661 accgacatat tcatggccca cgaccatcgg caccggaatg gttttttgcg accactcatc
    18721 ccagttatag atgtgaacgt cagtcccgca gatggctgtt ttacggattt taatcagcag
    18781 atcgttatgc ccgagttccg gtacagggac gtcggtcatc cagatgccct cttccgcttt
    18841 cagtttggat aacgctttca tctcacatcc tcaggcgata acgcccagtt gtttaccaat
    18901 acgcgtgaat gcttctactg cacgcgtaat ttgctcaggg gtatgcgccg cagacatctg
    18961 ggtacgaata cgcgcctgac ctttcggaac gaccggatag aagaaaccgg tgacgtagat
    19021 gccctctttt tgcagctctc gggcaaattt ctgcgccact accgcatcac caagcatgac
    19081 cggaataatg gcgtgatcgg ctcccgccag ggtaaagccc gccgccgaca tttgctcacg
    19141 gaactgacgc gcgttcgccc acagacggtc acgcaactcg ctacccgctt caaccatctc
    19201 cagcactttg atggaagcgg cgacaatcgc tggtgccagc gagttggaga acaggtacgg
    19261 acgagaacgc tggcgcagcc actcaaccac ttctttgcgc gccgcggtat aaccaccaga
    19321 agccccgccc agcgctttac cgagcgtgcc ggtgatgatg tcgacacgcc ccatcacatc
    19381 gcagtattca tgggaaccac gaccattttc accgacaaaa ccgaccgcgt gggagtcgtc
    19441 taccatcacc agggcatcgt atttatctgc cagatcgcaa acgcccttca gattggcaat
    19501 cacgccgtcc atcgagaaca caccatcggt ggcaatcagc acatgacgcg caccggcttc
    19561 acgcgcttct ttcagacgag cttcaagctc ctgcatatcg ttgttggcat agcgatagcg
    19621 tttagcttta cacagacgca caccatcaat aatagacgcg tggttcagtg cgtcggagat
    19681 aatggcgtct tccgcgccca gcagcgtttc aaacaggcca ccgttagcat caaagcagga
    19741 agagtagaga atcgcatctt ccatccccag gaatgccgcc agtttttgtt caagctcttt
    19801 atggctgtcc tgagtgccgc aaataaaacg caccgaagcc atgccgaaac cgtgagaatc
    19861 cattcccgcc tttgctgccg caatcagatc cggatgattc gccagcccga gatagttgtt
    19921 ggcacaaaag ttaatgacgt ggcttccatc agccacggtg atatctgctt gctgcgcaga
    19981 cgtaataatg cgctcttctt taaacaaccc ttccgcccgt gcggtttcca gatcgttggt
    20041 taactgctga taaaattctc cacgcattgc gattctccag acagggcaaa ttccagcaca
    20101 tattacccaa acttataggt cgcgacgaga tgatgcgtta acacttctgc aaaattcagg
    20161 ataaataacg tgtatcttga tgtctctcaa cacctacaga tagtagaaaa actgtacagt
    20221 tttggctgag cataatgatg agtgatttta ctgcattgat ataaaaagaa aaaatgatgc
    20281 caaagcatca tttttcctat ctcagttggc atcaaaacgc tacccactaa tggagggaaa
    20341 cctattttct tgaaaggaaa attctcagcg cctgcatttt tgcccctaaa gtgttactcc
    20401 tgaagcagcg aaacaaatcg aaccattttc ctctccccag atagtttagg ttgaagagat
    20461 cggaacgttt gtaatagcac atgacaaaaa tgccctggtc atcgtccaca atgttattgt
    20521 ttagtgttat tttttgactt tccagaacta atttatagaa ttctttccat ttatgctgtg
    20581 aaccaacaat tgcgccccca ataatatata catgattgcc aatcatgaaa tcaaaagcct
    20641 gttgttgaga tgtaacagta agcccttttt ttatagtgaa aagatgcatc ttattttcat
    20701 caaatgggaa atcccaaatt ttcaatcctc gagttacatt tggcttacgg caatagccaa
    20761 aatctatcca cgcaacaaga ggcgttttca ctaaacccat gttgatagct ttatttacaa
    20821 aataggactt aaggttacat accaaaacat actctggtga ccagtactct gggtttttta
    20881 attgtcgagg ctcaagtcta tttgtaaacg actcatcttt ttgaattttt tcgatacgac
    20941 ttctgatata tctaaatttc ttttttatat cgataacgat aaccgttgtt ggttttccgt
    21001 tgcgaatcgc ttcaatcctt ggtttcaaat cgggagaagt gaaaataatc atctcatttt
    21061 caagtgcggc cagacgctca aagtaactaa aataaacatc aactgaacga gctagttttt
    21121 cacggaaccc tttattagta gtccaatccc ctctaccaat gtcaaaatat gcagtgataa
    21181 ttgtggtgga tgatttcatt actaataaga ccattaatat aaaaaaagaa ttatatcata
    21241 aatattaaca gaatgtatac tgtatattat ccattcaaat gattccgtta tttatcatac
    21301 atcggaatat tgatactaaa gcactattca catgcaaaac caacatccgc catgaaggac
    21361 tagctaaaac ccaaactagt ttgttgcaat tagcatcctt gcacctctat gtaaagggct
    21421 gaagggattc ggatgtgatg gtatgattac agacattcgt gtctgagatt gtctctgact
    21481 ccataattcg aaggttacag ttatgatcat cgttaccggc ggcgcgggct ttatcggcag
    21541 caacatcgtt aaggccctga atgataaagg catcaccgat attctggtgg tggacaacct
    21601 gaaagacggc accaagtttg tgaacctggt ggatctggat atcgcggact atatggataa
    21661 ggaagacttc ctgatccaga ttatggctgg cgaagagttc ggtgatgtcg aagcgatttt
    21721 ccacgaaggc gcatgctctt ccaccaccga gtgggatggc aagtatatga tggataacaa
    21781 ctatcaatac tccaaagagc tgctgcacta ctgtctggag cgcgaaatcc cgttcctgta
    21841 tgcctcttcc gcagccacct acggcggacg cacctccgac tttattgaat cccgcgagta
    21901 cgaaaaaccg ttgaatgtct acggttactc aaaattcctg tttgatgaat atgttcgtca
    21961 aatcctgcca gaagcgaact cgcagattgt tggcttccgc tatttcaacg tttatggacc
    22021 gcgtgaaggc cataaaggca gcatggcgag cgtcgctttc catctcaaca cccagcttaa
    22081 taacggtgaa tcaccgaagc tgttcgaagg tagcgagaac ttcaaacgtg acttcgtcta
    22141 tgtgggcgac gtggcagatg taaacctgtg gttcctggaa aatggcgttt ccggtatctt
    22201 caacctcggc accggtcgtg cagaatcctt ccaggcagta gcagatgcta cgctggctta
    22261 tcacaagaaa ggccagatcg aatacattcc gttcccggat aagctgaaag gccgctacca
    22321 ggcgttcact caggcagatc tgacaaatct gcgcgcggcg ggttacgaca aaccgttcaa
    22381 aaccgttgct gaaggtgtaa cggaatacat ggcctggctg aatcgcgacg cataagagct
    22441 ctgcatgaaa atactggtga tcggcccgtc ttgggttggc gacatgatga tgtcgcaaag
    22501 tctctatcgc acgctccagg cgcgctatcc ccaggcgata atcgatgtga tggcaccggc
    22561 atggtgccgt ccattattat cgcggatgcc ggaagttaac gaagctatcc ctatgcctct
    22621 cggtcacgga gcgctggaaa tcggcgaacg ccgcaaactg ggtcatagcc tgcgtgaaaa
    22681 gcgctacgac cgcgcctacg tcttaccaaa ctccttcaaa tctgcattag tgcctttctt
    22741 cgcgggtatt cctcatcgca ccggctggcg cggcgagatg cgctacggtt tactcaacga
    22801 tgtacgcgtg ctcgataaag aagcctggcc gctaatggtg gaacgctatg tcgcgctggc
    22861 ctatgacaaa ggcattatgc gtaccgcaca agatctgccg cagccattgt tatggccgca
    22921 gttgcaggtg agcgaaggtg aaaaatcata tacctgtaat caattttcgc tttcatcaga
    22981 acgtccgatg attggctttt gcccgggtgc ggagtttggt ccggcaaaac gctggccaca
    23041 ctaccactat gcggaactgg caaagcagct gattgatgaa ggttatcagg tggttctgtt
    23101 tggctctgcg aaagatcatg aagcgggcaa tgagattctt gccgctttga ataccgagca
    23161 gcaggcatgg tgtcggaacc tggcggggga aacacagctt gatcaagcgg ttatcctgat
    23221 tgcagcctgt aaagccattg tcactaacga ttctggcctg atgcacgttg cggcggcgct
    23281 caatcgtccg ctggttgccc tgtatggtcc aagtagcccg gacttcacac cgccgctatc
    23341 ccataaagcg cgcgtgatcc gtctgattac cggctatcac aaagtgcgta aaggtgacgc
    23401 tgcggagggt tatcaccaga gcttgatcga cattactccc cagcgcgtac tggaagaact
    23461 caacgcgcta ttgttacaag aggaagcctg acggatgcgg gttttgatcg ttaaaacatc
    23521 gtcgatgggc gatgttctcc atacgttgcc cgcactcact gatgcccagc aggcaatccc
    23581 agggattaag tttgactggg tggtggaaga agggttcgca cagattcctt cctggcacgc
    23641 tgccgttgag cgagttattc ctgtggcaat acgtcgctgg cgtaaagcct ggttctcggc
    23701 ccccataaaa gctgaacgca aagcgtttcg tgaagcgcta caagcagaga actatgacgc
    23761 agttatcgac gctcaggggc tggtaaaaag cgcggcactg gtgacacgtc tggcgcatgg
    23821 cgtaaagcat ggattggact ggcaaaccgc tcgcgaacct ttagccagcc tgttttacaa
    23881 ttgtaagcat catattgcaa aacagcagca cgccgtagaa cgcacccgcg aactgtttgc
    23941 caaaagtttg ggctatagca aaccgcaaac ccagggcgat tatgctatcg cacagcattt
    24001 tctgacgaac ctgcctacag atgctggcga atatgccgta tttcttcatg cgacgacccg
    24061 tgatgataaa cactggccgg aagaacactg gcgagaattg attggtttac tggctgattc
    24121 aggaatacgg attaaacttc cgtggggcgc gccgcatgag gaagaacggg cgaaacgact
    24181 ggcggaagga tttgcttatg ttgaagtatt gccgaagatg agtctggaag gcgttgcccg
    24241 cgtgctggcc ggggctaaat ttgtagtgtc ggtggatacg gggttaagcc atttaacggc
    24301 ggcactggat agacccaata tcacggttta tggaccaacc gatccgggat taattggtgg
    24361 gtatgggaag aatcaagtag agtgtcgttc tacaagtatg tctcttgcag atttgccagc
    24421 tcaaaccgtt tttcaaaatt taaatcttga aataataacc aataagttga catcggagat
    24481 aagatgacct caacattatt tttctctctc gagaaaaaaa actggatagc gtactggaac
    24541 agagctctcg tattcttatt cattaccacc tattttttgg gtgggattac aaggtataaa
    24601 catcttattg ttattcttat gacaataacg acaatcgtct atctctgcaa acggccaaaa
    24661 cactatctct cactatttaa aacatttctt tttggtagtg ttgccatatt aactattgct
    24721 gcattgctgt cacttcttca atcccctgat gcaggtgcta gcatgaagga aattttcaaa
    24781 gctattattg agaatacttt actatgcaca atagcaatac cggtcatatt gagagacgag
    24841 aaaagagaag atgtcgaaaa aatcgttttc ttctcattta ttagtgcgtt gggcttacgc
    24901 tgtttttctg aattgattac ctattataag gactatcaac aagggataat gccattcgca
    24961 gattatagac accgtagcat ttctgactcg atggtctttt tattccctgc attgttaaat
    25021 ctctggctta tcaaatcagc aaaataccgc atttcttttg tggttctaag cgttattttt
    25081 atttttctga tattaggaac tttatccaga ggggcctggc tttccgtgtt agtcattgga
    25141 ttaatatgga ttctgatgtt taaacaatgg aagttactat tagtaggagt aatggtttcc
    25201 atcattgcat tgtcggttat tttcacacat aaggagatga ccgcaaagct aacgtataaa
    25261 cttcaacaaa ctaatagttc ttatcgctat gcaaatggta ctcaaggcag cgcactcgat
    25321 ctaatattag aaaatcctgt tattggttat ggttacggta acgttgcata taaagatgtc
    25381 tataataaac gtgtcattga ttatccagaa tggaccttta gacaatcaat agggccacat
    25441 aattttgcgc tattcatctg gtttggcact ggtttattag ggctggtaag tcttatgatg
    25501 ctatactgtg caatattgaa agagtgtata aaaaatggcg tcaagaataa atatcgctca
    25561 ccatataatg catattatat aatcttacta tcttttatag gttattttgt tatccgtgga
    25621 aacgtagaac aaattgaacc aaatttatta ggcgtttacg ccggcttatt attagcgatg
    25681 aaaaacaagt aaaaaataaa aaaggctgca taatgcggcc tttttattta ttcaaaccaa
    25741 ttatgaataa cctcttcgaa tctttgagtt acgccatccc aactgtagtg atcaaacaca
    25801 aagtcctgac cttgctttgc aactgctgtt aattcaggat tcgctaacgt ttttaaaata
    25861 tcgctgctta tggagtctgc agtcataggt tcttttaaat ggaatcccgt agtattttct
    25921 ttgacaaatt ccgtcatccc cccacgcgta ctcactaaaa caggcttacc agcacccata
    25981 gcttcaattg ccaccatgca aaatggttcc tggaactggg aaggaatgac gactaaatct
    26041 gccagtgggt aataacaatg catcttttca ggcggtatgc ttcctaacat aatgcaacga
    26101 tccttcaaac gttttgcaat ttccctgaca ttgcgttgat atgcaccttt gtcactttta
    26161 ctcatttctg tataatcacc aatcacaact aattttaaat ttttgtgagc agcggccaat
    26221 ttttcgaatg attgcatcaa cagcaatatg cctttgtcag ggacgatacg cccagcataa
    26281 aaaataattt tctcttcagg ggagatatta agttcgcttc tagtgatcgg ctgaaaatta
    26341 gattgatagg tttctaaatc aattccattt ggtacaatct caatatctgc atttgctaaa
    26401 taagattggt aatacttttt caagtataaa ctagggacta tcattttaac attttgctca
    26461 agcaattttg gttcaaatgc attatgcata tgtataacca ctcttgcctg aggagctcgc
    26521 ttacgaattt gagtgtatag cttcatgcta ttatgaacta caataacact ttctttcgtt
    26581 ataggaaaat catgtgcaat attcaatatt ctctgtgaat atggaagagg atcaagtctt
    26641 gtccattttt gaaataaccg cttatatatg cgactgaatc caaccctatg aatactacaa
    26701 ttatcattaa caaaggtgta atttgagtat cctggatttt tgatacaaac aatacgattt
    26761 ggaaaattgg tacgctgagc aacctgatac atccacgttt caacagcagc cgctccgcga
    26821 ggaggaattg aaaaaatagg agtaaccgta aatattattt tatcaaccat ctcgtatatt
    26881 aacctttcat aacattatat ttaattgctg taaaaacacc atcgagaaat tttttctggt
    26941 aaagcaagtg tttatatttt tctttgtgct catgtttttt aacagctttt ttataaggta
    27001 tatttctcca tggtgatata ttataaatat tacgaaaata atctgcagag gcgtagttcg
    27061 cccaatcatg ccatggctta gttataccag tataatgtat gaagacagtg tcatcattaa
    27121 taaaccgggt gtaatattca ctattttttt cttcaaattc tgactttata gtataaatac
    27181 aattatattt tctcggtaat attttagcat gatgtagaaa cattaagttt aaaatatctt
    27241 gatccgggta ctttaatttt tttataattg actcatctga taatagatca aagaattttt
    27301 ccgttagtct ttgttttaac cattcccgta aattaatata cattacacca gagttaaaat
    27361 agctaccgtt aaaatcctca ttacacaaac gctcagcgct tttactttgc atagcatcta
    27421 catcaattac cactgcccca tattcatctt taaactctaa tgctattaac tcgttcaatg
    27481 aacccttaca gacgatatca gcatcaagat ataataatga atccaatcgc gcagaaaaat
    27541 aatcaaatga aagcaaacga taataaatag atactggcca gatatttgat tgaggtagcg
    27601 ccttcaatgg ctcagaatca atatgatata atttaattac tgtttgatac ttcgttgcta
    27661 attgtgataa taattcaata tactctttgt ctaagtaatc agcgattatg tgaaaggtaa
    27721 atttgttttg tttattcttt tcaagaattg aagcaattga aatcgcagcc ccaacctggt
    27781 agttctcatc aataccccat gagacattaa acgtttcccg ttcatcaagt ttaacggggc
    27841 gcttgtccag ttcaattata tctttaaatt gagtaagatg tttaaaatcc aatttaatta
    27901 cccttcatca taatcattat ttgctcttca atcctagttt gacttttaca tccctgatta
    27961 cttttttaat tttctttttg aatataagat atgtatagcc aaagtctttc aattcatttt
    28021 ttatattata gtgccgttca agatcaatgc gatcttttgc ttttaaaaca gcggttgttt
    28081 ttttacctga aaggtcaatt agtcttaaac ctttttcgga tactataaaa tttcctttat
    28141 gaggatcgcc agacaccatt ccatgctgat gcaactcttt tatcgattct gataattgat
    28201 ccttcagatc ttcagaaatt tccaaatact catttaaccc gacaccttca atatactcaa
    28261 tgagcataat ataataatga gcgaaattta atgttttacg ttcagctagc aaaaaatagt
    28321 cattaattga ttggatgcct tcaccacgta cccgatcggt ttgataaatt aaattttcat
    28381 aataatcttt ttttatgcaa gattttaaga aacgttcggt cattttatgc tttggcgcat
    28441 aaactttaag tacaagaggt ccacgagcag tgtcaataag aatgacttta gtatcatcaa
    28501 tagaacgaaa aacttttaat gttttaaccc tgcaagttaa aaagtcattt agaacttgct
    28561 cataaaaggg atcattatct tttatgaaaa ctttcaaccc atttatcgtt ttattatata
    28621 tcataatgaa ttattttaac cttaaatctt tagaagcatt tttctttata atactttaaa
    28681 taattaaaaa tgcctgccat ataatgcttt tgtttaaatt tatgttttgc acaataccga
    28741 tactgattgc tatttacagg tttaagtaaa tcttctttac tccacggaga agctgctttg
    28801 gcgatcaaaa aactacgtga aaccggataa tcagcccact catgccaggg ttttgtaggt
    28861 cccacatagt gtataaacac ggtgtcatca ttgactggat tatcaacttt gtcttttaat
    28921 tcatagttaa tactatatcg ggtattatat ttctccgaaa taaacttcac tttaccattc
    28981 aataatacat tcagtacatc ttgatcaagg tgggtgattt tacttaccca atctgggtca
    29041 cgcaacattt caatagcttt tgacgaaatg ttatttagat tccactcatc aatatttatc
    29101 agtaaaaaac cagcattgaa atatccagaa gctaactgtg gtgtagttaa taccgaggct
    29161 cgattttgcc accattcaat atctctttca gctacaactg cggcaatttc attagtagaa
    29221 aattgataat ctaagagttc tttaatacta cccttgcaag caatatctgc atcaagatat
    29281 agtatttttt catgtttatg atagaaataa tctgcaatta taaatcgaaa atatgttgcg
    29341 taagtccagt ttttcgtgct tggtaatgac ttcagcttat cacaattgat aacataaata
    29401 ttaattcttg aattatattg ttttgctaaa tcagaaaaat ataatttgtc tttatcactg
    29461 atataatctg tgaaaacatg aaattcacaa gagatttctc tattgtttaa tagaatagat
    29521 gcgatggcta caccacatcc aaaaagaaaa tttctgtcaa taccaaatgc tatatcaaga
    29581 gtatttttag atcttatatt ttgataatga aaattatact caaaaaggat gatgtcacta
    29641 tcattgagtt gagacatatt tattctgtat ccaaaatttt ggcaaaaata agttacgggt
    29701 tacaacgatt ttcgaatcgt tctttccctg atttttgtgg cttttgcttc tgcttgcgac
    29761 agcagtcctt gttcctgctt gagaatgtct ttaagcgggg cggcaaaata caccttcata
    29821 aagcgccaga tatcccgctg agtcaggcca atattcattg aagaaaaata aagccctata
    29881 agatctttat cgcgccaacg acctggaacg cgcgtgcgaa gctgcgcccg gtgtaggtca
    29941 attaccgaaa tttttaactc ctcttcctta ccggaaaaag gcaagtgcag caggaaatga
    30001 cagatataac agtcacgatg gttaatgccc gcagcatgca tatcgcgcac catcgtcgcg
    30061 acacgcttaa taagcatacg ctttacgcga acatctggtg ggttggtcgc ccagtcagca
    30121 cagtaatctt ccagactgat ggttggtgtc agatcttcgg taataataaa cgaggttctg
    30181 gtcagcggat tcatgccttt ttcgccgaat gccaccccat acatagtatc aacgccgaca
    30241 tcccgcagtc gatgaatcgc attccattcg cggtctgcgc ctaatactgg catccgcaat
    30301 gagagtaaat ttttgattat ctctttcagg gtcgtgccgc gatgccattt gagaaaatag
    30361 ctttttcccg ccatttcaaa gcgcagagta cggcgagttt ccagttcacg aaatacctca
    30421 ccctgcaagg ttttaacttc ctcaaaagga tctttgccgc gccataacgt ggcaaacggc
    30481 tctttaagtt caaccatcca gaccacccgt tatgatatcc gccgctttct ctggcagact
    30541 gtataaatct tgtgtatcag cataatgtcg cgcattttcc gcccaagcct ggcgcaatga
    30601 agattgcgtt aacgctttgc gtaaaatctc attcaatgtt tcctggcgga atggctcagc
    30661 aatagcctcg ccgcaattag cgtcgacaat ataatgcgca tagccacaaa cggcagttgt
    30721 tagtaccggt aatcctgcag taatcgcttc cagcagcaca attcccgccg cttcctggta
    30781 ggcaggatgc agtaataaat ccgccgccgc cattaattcc gagacatcgt tgcgccccga
    30841 gaagaagtga acattactgc gcacgccgcg tttttctgcc agtgcctcaa attttcgcgg
    30901 tttatcctgc ccaacaacat atagcaatgt gttgtggcgc agcgaatccg gtaacgaagc
    30961 aagtgcttca atggaacgat cgacaccttt acgcgtgaag tctgaaccga cctgcaacaa
    31021 taaatattgt tgttcggtta ttccattctt cttacggaag atttcacggc tattggctgg
    31081 ctgctggcta tatttacgat caggataaat ccccggtggc agaatatgaa aacgctccgc
    31141 ttcagtctga taatgtttct ggaaatcggc gatttgctta tctgtcagca tcagcagctg
    31201 tgtcggcttg ccctgttcga aggttgcccg ctcaaaggcg gcataatggc gataacgtga
    31261 cgtcaggcga tagaaaaagc ctttttcctg cgctactttc tcggcataac aaacatcagc
    31321 ggcataataa acgtccagcc ccggcatttt gttgaatcca acgactttat cgacgggatg
    31381 ttcgcgtaaa tgtttttgca cccaggcaaa atactccgca ttgcgcccgt gattggtatg
    31441 cgatttaacc ggcactttga tcagttcaaa tacatcaggg cattcgcctt cccacgactg
    31501 ggtataaacc cgaacatgat gacctcgggc ggcgactgtc tgagcaatac gcataaaatc
    31561 gcgctgcaaa ccgccaaagg gaaaatattt atataaacaa aaagcaacga tcataacgac
    31621 gtacctgtcg tggaggaggg cagtaattta tcgacagcag caatgacatc tgccgccgga
    31681 ataaccgaaa gatacatctc atttcggtca cgctgatcgc gcgttggcat ttcccggtaa
    31741 tctcccgccc agaattgaat catgttattt gaccagggac gccagaaaat atgatctgtt
    31801 gcgccaaaca gcgatatcag cggcgtatta actgcagcgg caatatgcgc cggtgcggaa
    31861 tcaacgccaa taaacagctg cgcatgatcg attaacgcac caagttccgg gaaggtcacc
    31921 tttccagcca gcgccgttac tggtggcgtc tggcatccct gcgcaatttc attgacgcag
    31981 gccagatcgt ctttatctgg gccggacgtc agaacgactt cataaccacg agcatgtaag
    32041 gcatcaatca cagcggaaaa cttggcgttg tcccagcatt tgaagatttg ccgcgccgta
    32101 ggttggataa ccacataatt ttgtccaaca ccagcgtgat caagttcgcg acgcatacgt
    32161 ttccagcttg caggcgggta actcatggtt gtctgcttca ccaacgaatc aagtcccaat
    32221 ggtgtcagca cggataagtt actttccacc acatttccac cctgcaacgg caccaaatgg
    32281 gtgaaacttt tacgccaaaa agcagactgc cgatgatgat aatcctggga aattttcaca
    32341 cgagcattta ataagcgaac cagtatagca accatccatt gatcggtaag attgacgata
    32401 aggtcatact tattggcacg taataccttg atgagatgaa aaaagttggc aattttttct
    32461 gaggcttttg cttttttatt ttttatgccg tagagcgcgt taatctctgg attttcagac
    32521 aggatcggga tggtgtcctg ataaagcagc acatcgattt ttgcgtcagg gtaatttttt
    32581 ttcagagaac taatgacggg agtagttaat aacatatccc catgaaaacg catcttaatg
    32641 agcaaaattc ttcgaaatgg cttatccaca agtaactctt tttgttaaga ttatccgatt
    32701 aaggtgaagc agaaaaagca cgctaccgcc ccaggctcaa cagctacctg aatactgata
    32761 ccgcactgat attgacgcgc tagtgtatca cttctttcgc ggcaatccga cccgaatgat
    32821 ttttaagcac attgttgatt ttcaataaag aaatcaatca aataaaatca ttagcaacca
    32881 tattctttca ctgttgctct gcgattcctt cctgcacgta cagcagaaat caatgaatcg
    32941 cataaaaata caaaaaaagt atagatgttc agcaagaatt ggcgggtaaa ttgctattta
    33001 agtcaa
//
LOCUS       DAJTXY010000032        32775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTXY010000032 DAJTXY010000000
VERSION     DAJTXY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.032"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..328)
                     /gene="yfdR"
                     /locus_tag="OO566_001614"
     CDS             complement(<1..328)
                     /gene="yfdR"
                     /locus_tag="OO566_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6604936.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDSVIR
                     EKYGLPP"
     gene            complement(456..1280)
                     /locus_tag="OO566_001615"
     CDS             complement(456..1280)
                     /locus_tag="OO566_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HCR6604937.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(1346..1726)
                     /locus_tag="OO566_001616"
     CDS             complement(1346..1726)
                     /locus_tag="OO566_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604938.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGH"
     gene            complement(1723..2033)
                     /locus_tag="OO566_001617"
                     /pseudo
     CDS             complement(1723..2033)
                     /locus_tag="OO566_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2309..2605)
                     /locus_tag="OO566_001618"
     CDS             complement(2309..2605)
                     /locus_tag="OO566_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604939.1"
                     /translation="MLKQVYEMKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIM
                     AGVVFSLRRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            complement(2778..3452)
                     /locus_tag="OO566_001619"
     CDS             complement(2778..3452)
                     /locus_tag="OO566_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HCR6604940.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            3543..3743
                     /locus_tag="OO566_001620"
     CDS             3543..3743
                     /locus_tag="OO566_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6604941.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            3787..4338
                     /locus_tag="OO566_001621"
     CDS             3787..4338
                     /locus_tag="OO566_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604942.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDEQYLSISKLQEHAALVYKIFCVSE
                     SSDARECAAPGAVACRDCGETNA"
     gene            4514..4693
                     /locus_tag="OO566_001622"
     CDS             4514..4693
                     /locus_tag="OO566_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6604943.1"
                     /translation="MRQVNRWFKEHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            4683..5624
                     /locus_tag="OO566_001623"
     CDS             4683..5624
                     /locus_tag="OO566_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6604944.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            5621..6115
                     /locus_tag="OO566_001624"
     CDS             5621..6115
                     /locus_tag="OO566_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6604945.1"
                     /translation="MSMSLLNDVQKFIEAHRGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            6115..6768
                     /locus_tag="OO566_001625"
     CDS             6115..6768
                     /locus_tag="OO566_001625"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCR6604946.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVLTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            6765..7091
                     /locus_tag="OO566_001626"
     CDS             6765..7091
                     /locus_tag="OO566_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR6604947.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            7088..7477
                     /locus_tag="OO566_001627"
     CDS             7088..7477
                     /locus_tag="OO566_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6604948.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITINKYEKTAGAAA"
     gene            7497..8294
                     /locus_tag="OO566_001628"
     CDS             7497..8294
                     /locus_tag="OO566_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6604949.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSQDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVSRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNISSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            8302..9291
                     /locus_tag="OO566_001629"
     CDS             8302..9291
                     /locus_tag="OO566_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6604950.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            9306..9674
                     /locus_tag="OO566_001630"
     CDS             9306..9674
                     /locus_tag="OO566_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCR6604951.1"
                     /translation="MMRDIQMVLERWGAWAASDSSGVDYSPIAAGFKGLLPYTSKKRL
                     ACSDSDALIIEGCLARLKQKRPYEHSLLVAHYLYRISKRKIAKARGKDEKLIRIEIQL
                     AEGFIDGCLSMLDLTLDLDV"
     gene            complement(9703..11034)
                     /locus_tag="OO566_001631"
     CDS             complement(9703..11034)
                     /locus_tag="OO566_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HCR6604952.1"
                     /translation="MLDTLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTG
                     KTEFSIKLKNLIIEQETESKVVYIDAFKGDHAESPLLLITSAIASILPEEEKQHFIKR
                     SLPAIRFGLKTVLKAGAGWFLRQEASEVSEEFQDAMKKASNAAIDGTIENLLEDHMES
                     EKNINSLKSCIESISESQKIVIIIDELDRCKPSFSTNVIETIKHIFDINNVFFILVTN
                     TEQLKASINHIYGYSINSQKYLDKFIKYTITLPDTCLINGHVVCKTSVIYWNYLVDKT
                     KSLNEINRLSGRFIRDLIQRTNLSLRETQTFSRNLNIFQLLNDDEYKSSDPLINKIFV
                     VAVFIHCFGDKEKLKQEITAESISHLADLLNIKEIPYSYERRSQIPEISIVFFGIIKD
                     SITLNERFAPKSDEELKKFTTVYTDYEHLNFWSTTPRELLIKYINQMSFIQ"
     gene            11331..11657
                     /locus_tag="OO566_001632"
     CDS             11331..11657
                     /locus_tag="OO566_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR6604953.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            11661..12137
                     /locus_tag="OO566_001633"
     CDS             11661..12137
                     /locus_tag="OO566_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR6604954.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLALKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKEAGGVVNEAEL"
     gene            12121..12513
                     /locus_tag="OO566_001634"
     CDS             12121..12513
                     /locus_tag="OO566_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCR6604955.1"
                     /translation="MKLSYKLLIVGFFVTVIGSFIWSANHYYSKYQYEKKRADEAVRN
                     AESATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRAREDIKVAVADDDCASRPVP
                     DVAADRLRKYADSIRAGSNDAVTDEPAR"
     gene            12976..13308
                     /locus_tag="OO566_001635"
     CDS             12976..13308
                     /locus_tag="OO566_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604956.1"
                     /translation="MPVNIDPEKLNDEREQVIAKWLFKDVDLISQQIELGEENVKRFD
                     ELLSIFDCCQSSWFATEHLFDNTELEKVWHEFESNFNKYINGGESKDLLMKMLDKLIS
                     SRFVFESR"
     gene            13359..13709
                     /locus_tag="OO566_001636"
     CDS             13359..13709
                     /locus_tag="OO566_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6604957.1"
                     /translation="MPPRIPKACRVRGCRSTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRVRVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKEKTARERLK"
     gene            13835..14329
                     /locus_tag="OO566_001637"
     CDS             13835..14329
                     /locus_tag="OO566_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6604958.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEELPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            14563..16059
                     /locus_tag="OO566_001638"
                     /pseudo
     CDS             14563..16059
                     /locus_tag="OO566_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            16071..16253
                     /locus_tag="OO566_001639"
     CDS             16071..16253
                     /locus_tag="OO566_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604959.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            16253..17494
                     /locus_tag="OO566_001640"
     CDS             16253..17494
                     /locus_tag="OO566_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6604960.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADDIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPSCVVPKLNSSWEPVYQVTFPDGSADVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            17472..18122
                     /locus_tag="OO566_001641"
     CDS             17472..18122
                     /locus_tag="OO566_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6604961.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRTKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYIALSLRDAEDVGSALNALKNLNF"
     gene            18137..19342
                     /locus_tag="OO566_001642"
     CDS             18137..19342
                     /locus_tag="OO566_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6604962.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            19392..19592
                     /locus_tag="OO566_001643"
     CDS             19392..19592
                     /locus_tag="OO566_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604963.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            19595..19918
                     /locus_tag="OO566_001644"
     CDS             19595..19918
                     /locus_tag="OO566_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR6604964.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            19915..20325
                     /locus_tag="OO566_001645"
     CDS             19915..20325
                     /locus_tag="OO566_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6604965.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRLRVDMPADNFGVEPQYL
                     ITFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECGQSHGGNNDDFLFAR"
     gene            20300..20806
                     /locus_tag="OO566_001646"
     CDS             20300..20806
                     /locus_tag="OO566_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604966.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            20803..21363
                     /locus_tag="OO566_001647"
     CDS             20803..21363
                     /locus_tag="OO566_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604967.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            21372..21539
                     /locus_tag="OO566_001648"
     CDS             21372..21539
                     /locus_tag="OO566_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6604968.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDEL"
     gene            21526..23019
                     /locus_tag="OO566_001649"
     CDS             21526..23019
                     /locus_tag="OO566_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6604969.1"
                     /translation="MTSFNTIPSNTLVPIFYAEMDNSAANTAQDSGASLLIGHANNGA
                     EIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATVT
                     LTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPALPFTASSSAG
                     VVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAAM
                     ADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAGD
                     QFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKGK
                     RFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAYV
                     LRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYEL
                     FKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            23019..23375
                     /locus_tag="OO566_001650"
     CDS             23019..23375
                     /locus_tag="OO566_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6604970.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPASKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            23375..23644
                     /locus_tag="OO566_001651"
     CDS             23375..23644
                     /locus_tag="OO566_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6604971.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            23786..25621
                     /locus_tag="OO566_001652"
     CDS             23786..25621
                     /locus_tag="OO566_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6604972.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETISPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYIPYPAVDLAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            25682..27010
                     /locus_tag="OO566_001653"
     CDS             25682..27010
                     /locus_tag="OO566_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR6604973.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEENAGTGRRVETHEYPNRDKPYTE
                     DLGKVTFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKGEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDVARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVEQATTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            27007..28086
                     /locus_tag="OO566_001654"
     CDS             27007..28086
                     /locus_tag="OO566_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR6604974.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEISRQWPGD
                     GGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRELVQIAQALAAPFGIEVVNNGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEDPGARKKKKARVQEDPF"
     gene            28086..28634
                     /locus_tag="OO566_001655"
     CDS             28086..28634
                     /locus_tag="OO566_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR6604975.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCLTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            28634..29059
                     /locus_tag="OO566_001656"
     CDS             28634..29059
                     /locus_tag="OO566_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6604976.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYASRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            29046..30104
                     /locus_tag="OO566_001657"
     CDS             29046..30104
                     /locus_tag="OO566_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6604977.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDEFWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRLPIACSTAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWAY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGADGEYSHQLLAPVD
                     NISIAKNELAVLGTISWT"
     gene            30095..30679
                     /locus_tag="OO566_001658"
     CDS             30095..30679
                     /locus_tag="OO566_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR6604978.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSSSDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGIQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRQDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNSTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            30683..31333
                     /locus_tag="OO566_001659"
     CDS             30683..31333
                     /locus_tag="OO566_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6604979.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            31305..31739
                     /locus_tag="OO566_001660"
     CDS             31305..31739
                     /locus_tag="OO566_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HCR6604980.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYLTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(32512..>32775)
                     /locus_tag="OO566_001661"
     CDS             complement(32512..>32775)
                     /locus_tag="OO566_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6604981.1"
                     /translation="LIIFNIFIAKIICGNDFIVRAGRNTLNICGMEYITKLFIPMALA
                     IIGFSVTIPNPICAIIYTCIYVYVSDKIGHWLSRTVGGPFLIK"
BASE COUNT         8082 a         7723 c         9075 g         7895 t
ORIGIN      
        1 ctgggggtaa tccgtatttc tcacggatta cggagtctat tttttcttcc atttgtttat
       61 agtcaggaag aaggcgtttc agtggtgcgg gaatgtcctg gcaatacgct tctgttgcat
      121 catgcattaa cgcttcaaaa gcaaattcct gcggcaccag ctggctgcaa agaaccgcat
      181 gttgggcgac gctgtagaag tgcgaaagat gaccggcaaa gcgacagata tttgaaaggg
      241 aaaccgcgat atcgttaata tcgatgtcgt ctttatttat cctgtcataa taaaaatgct
      301 tcccggaaaa agttttaata aatgacattt tgttctccac gtatatgcgc tgcaccgcgc
      361 tgaattctgg taaaaagaat ccctcaccat ccggcgatta ttgagtaaat tacgtttcca
      421 taaatgcccc cgcaggggca tttgcagtaa tgaaatcagg cggtgaaagt accaataaag
      481 gtttctactt tgctgtcctt gaatttctca acaagcagat cacgaaattc gttagccatt
      541 tcttcctgca ccgcctccag ctgaataatg cgcagaacca gtaccggacg atcgccagtg
      601 ataatgctga ggcgtaattt aaacggacgt tctttcagac cttcaaacgg aatgcattta
      661 aattcaaatg ccactggcat aatgtctttg gtcttcgctt cgacagactc catcagggag
      721 cgtttgccgc tgaagtcatt atcttcaaaa tcagcggtct ggtttgcttc aatcgtgatt
      781 ttacggaccg ccgcagccgc ttttgttgcc tgaatggtgt caccattagc atcaaagccc
      841 acaaggtagt cggcccagtc ttcaatccat tctgccagtg atttctggga gttacgctcg
      901 ccattaacag acaacagagc agagaacggt gctgtctttt tcagtttgag agtggcggtg
      961 ttatctgcgt gacctggttc atcaatagta cccaggttaa gcacactgac ggcacgcata
     1021 ttatcagcat cgataaagca gcgggtgcct tcatctgcaa gatctttaga ataacgggta
     1081 aagtcatcga tgctggcagt ggaaagcgca ccacggaaac ggaagcgatt taaattaaat
     1141 ttttccagat catgaatgcg gaaattctca ggcaatgcca cagcatcggc accaatctta
     1201 ctgataattt cattaacacc ctgagcagaa ataagggcat ggatttgatt aattgcggtt
     1261 gcgtctaagt tctgagacat aataagtcct aactatataa agatattcag tgatgagata
     1321 aataatcagt taattaagaa cgatattaat gacctgctgc gcggagtttt ccgtcaggtt
     1381 caccggcaag agtcagtaat tgtccctggt cttcctgcag aatagtcagg cgaccaccgc
     1441 gattgacata catcggcgtt tcggtggtgt cttcttcgga aattttcccg cggttagtcg
     1501 ggcgaacata tgagagtttg tgtttgattt tcacacggtt ctcatcaaat ggttcgattt
     1561 ccaggttgag tgagacctta cctttggttt tcgtgttcat cacaccggaa gcgacttcac
     1621 tgagaactgc gccgattttg gtttcaaata cgccgccgtc cagctccccg ataaatgcct
     1681 gcacatcagt actgcgttcg ctagccattt tgctgctcct catcatatcg accctgcaag
     1741 gccgattagt ttctccacaa aacagagaag aacacctgcg gtagcagccg cccggatgga
     1801 ttgggttatg agcccgtcgt ccggtgatgc tcttctctgt tttgtaaaaa ggacggtacc
     1861 agccggaagc aagggtacaa actggtaccg ccaggactac acacagcata aagttgtggt
     1921 gccgggtgcc tcccggtgcc tggcgaaggt tgcacaccag acgggtgggt atccacagaa
     1981 ggtcgactgt cagcctcaac cttaacccgc gtgcgctgag ccgcattcac cacaactcta
     2041 aggattctct ttggttgaaa atacttagct gttatgtgcc tgtcttttca ccacttcagg
     2101 ctcggtggta tccttttaag cccgtataca taaaaggaaa atcaaatgac ttttgatgaa
     2161 aaagaacttg ataatgcaat taataaaatc atcgtaacgt cgctcttttc ctgtctcagc
     2221 gacactcagc agaaacagtt ctacgaatcg gctttcaaca tgatcgagcg ttgttgtttc
     2281 tgcgatgccg acgagttacc tgaaaaaatc aggaaacagt tggctgatgc tcttcgagtg
     2341 cgactttctg accaattttc tgaaatgtgc tctccgaatt tggacaaata gaaaaaggcc
     2401 atttccattc agggtctgat ggaaatactt cagcctgttc caaagcacgg cgtaaagaga
     2461 acacaactcc agccataatc tgatgtttcc cattggtcca gctatcgccg ctctgatcta
     2521 caggggcggc tatgtcgtat gaccaaacga cttcacagtt attgtttaaa atctggactt
     2581 tcatttcata cacctgcttt aacatgagtg cctagtggca caacatgact caacgaatca
     2641 tcctggactt catatgcccc aggcggctac ttcgtgggcg tcctgcctgt tcgttgtttc
     2701 gcttgggtac attatgtatc ccaagggtac attgtcaagt ataaaaaaac ctgccgaagc
     2761 aggttcataa acattgatta ggctttgatt ttgtatcttc ttggttttcc tgagaaaatc
     2821 actgtaccaa ttatagagca attaccgttg atcttaatgt aaggctcagg ccagtttggg
     2881 tttaacgctt tgagataacg ctgtgtccca tcttctatca accttttgaa ggtggtttcg
     2941 cctgtatcgt gcatcaatgc aataacgtcg tcaccgtggc aggcaggtac ttcaggatcg
     3001 acaaaaatca tgtctcccgg gcggtactca tcaatcattg aatcacctat cacccgcaag
     3061 atataagtca tttccccaca gggtacaggg cagggatacg tttctgctgt gctcaaatca
     3121 acctcagaat atccaacttc tttccatgct ccggcctgta cccatgatat gacagggact
     3181 aatgtgattt gtttattagt gattgaaaca tcaggttttt ttgtgatgtt cgttgtctgg
     3241 tgttcttgat cgagccatcc gacaggcagg tcgaaacatt tttcgatgtg tcgtgccatg
     3301 ctgtcaccga tatttttagt agcaccatct cccataaacc tgctggtctg ggttggctcg
     3361 cgatcaatca tagtggcaaa ggaagaattc ccgccaacac catctctcag ttttctggcg
     3421 ttagaccgcc ggatgtcatg gattgttttc ataacgaaat taaaaccctt gtaccgttaa
     3481 ggtacaagtg tcttggaggt tcatttcaat catgtaacat gtacaccgga ggtacatatt
     3541 gtatgaaagc gtattgggac tctttaacca aagaacagca gggcgagttg gccggaaaag
     3601 ttggctcaac acctggctac ttacggctgg ttttcaatgg ctataaaaaa gccagttttg
     3661 tgctggctaa aaaacttgag caatgcacgt caggtgcaat tacgaaatct gacttaagac
     3721 cggatatcta tccgaaagat tagcagaaca ctttcaattt ttaaccacag aacgatgagg
     3781 ctaaccgtgg gtaagcatca ctggaaaata gaaaaacagc ctgagtggta cgtgaaagct
     3841 gtcagaaaaa ctatcgcggc gttgccgggt ggttacgctg aagcggctga ctggctcgat
     3901 gtaacagaaa acgctttatt caaccgcctt cgtgcagatg gcgatcagat tttcccgctg
     3961 ggatgggcaa tggttttaca gcgtgctggt ggcactcact tcattgctga tgctgtggcg
     4021 cagtctgcaa atggcgtctt tgtgtctctt cctgacgtcg aggatgtgga caacgccgat
     4081 atcaaccaac gcctgctgga agtcattgaa cagatcggca gttattcaaa acagattcgt
     4141 tcagcaattg aagacggtgt agtggaaccg catgagaaga cagcaattaa cgatgagcag
     4201 tacctctcaa tttcgaagct gcaggagcat gcagcactgg tctacaaaat cttttgcgtt
     4261 tcagaaagta gtgacgcccg cgagtgtgca gctccgggcg ccgtggcatg tcgtgactgt
     4321 ggagaaacta acgcatgaac agtttaacaa cacactaccg tcgctcgcaa ctgattgcgc
     4381 ttcctgtacc gggtggaaaa gcgaaggtgg agtattgcta tgcagtaaat gtaccaggtg
     4441 acagggaaat tgtaacccac agctttgcag agtgggctgt gggtgatttc aaccggcaga
     4501 aggagacagt cctttgcgac aagttaaccg ctggttcaaa gagcactacg gagtgcccgt
     4561 cagagtcatt cgttgggagc cggaaacaca acgtgttatc tacctccgcg aaggctatga
     4621 gcatgagtgc ttcagcccgc tcgaacagtt tcgtcgtaaa ttcagggaaa tagaggtcgg
     4681 tcatgagcac taaattaacc ggctatgtat gggatggttg cgctgcatca ggcatgaaat
     4741 tatccagcgt ggcaattatg gcccgcctgg ctgatttcag taatgacgaa ggtgtgtgct
     4801 ggccatcaat tgaaaccatt gcccgccaga ttggcgcggg aatgagtacc gtcagaacgg
     4861 ctatcgcacg gctggaagca gaaggctggt taacgcgtaa ggcgcgtcgc cagggtaacc
     4921 gcaatgcgtc gaatgtttat cagcttaacg ttgcgaagct tcaggcagcg gcattttctc
     4981 aactgtcaga ttctgacccg tcaaaatctg acgcatcaaa atctgacccg tcaaaatttg
     5041 atgcgtcgaa atctggcaaa aaagcgggtt ttcacccgtc agaatctggc ggggatccgt
     5101 cagtaaaatc aaaacatgat ccgtcagata aaaaaacttc tcgtccggac gcttcgcaac
     5161 cggacacgca gacggctgaa caggagtttt taactcgcca tcctgatgcg gttgtattca
     5221 gccctaaaaa gcgccagtgg ggaacgcagg atgatttgac ctgcgcacag tggctctgga
     5281 aaaaaatcat cgccctgtac gagcaggccg ccgaatgtga cggcgaggtg gttcgtccca
     5341 aagaaccgaa ctggacagcc tgggcaaacg aaattcgcct gatgtgtgtg caggatggtc
     5401 gtactcataa acaaatctgc gagatgtaca gccgcgtcag tcgcgatccg ttctggtgcc
     5461 gtaacgtgct cagcccgtcg aagctgcggg aaaaatggga tgagctttcc ctgcgcttat
     5521 cgccgtccgt cagcacgtac accgaaaaac gcgaagaccc gtacttcaaa gctagttacg
     5581 acaacgtgga ctacagccaa atcccggcag gattcagggg gtgagcatga gtcttttgaa
     5641 tgacgttcag aaattcattg aagcccatcg gggctgtact tccggagata ttgcggatgc
     5701 ttttgccggt tactcacggc agcgcgttct gcagtcagca agcaagttac gtcagagtgg
     5761 gcgtgtggct caccgttgtg aaggagatac acgcagacat ttcccgcgcc tgactgagag
     5821 agcgcaggaa ccggaaccac aaccagttcg tgaaaccaga cctgtgcgca atttctatgt
     5881 cggcactaac gatccccgtg tgattttgtg cctgacccgc caggcggaag aactggagtc
     5941 caggggctta taccgtcgtg ctgcaaccgt gtggatggcg gcattccgtg aaagccactc
     6001 ccagccagaa cgaaacaatt ttctggcgcg tcgtgagcgg tgcttacgga aaagcagcaa
     6061 gcgcgctgca tcgggtgaag agtggtatct gtcagggaat tacgtggggg cttaatgagt
     6121 aataaatatt gccaggcgct ggtggaactg cggaacaaac cagcccatga actgaaggaa
     6181 gtgggcgatc agtggcgcac gccggacaac attttctggg gaattaacac cctgtttggc
     6241 ccgtttgttc tggatctgtt cactgacggt gataacgcca aatgtgccgc gtattacacg
     6301 gcggaagaca acgcgctggc gcatgactgg tcagaacgtc ttgcggagct taaaggtgct
     6361 gcctttggta atcccccata cagccgcgcc agtcagcatg aggggcaata catcaccggc
     6421 atgcgttaca tcatgaaaca tgccagtgcc atgcgtgata agggcgggcg ctatgttttc
     6481 ctgatcaaag ctgccaccag cgaagtgtgg tggccggaag atgcagatca tattgctttt
     6541 attcgcgggc gtattggttt tgaactgcct gtctggttta tcccgaaaga cgagaagcag
     6601 gtactgacag gagctttttt cgctggtgct attgctgttt ttgacaagac ctggaaggga
     6661 ccggcaatca gctacatcgg gcgcgatgaa cttgaggcat gtggtgaggc gtttctggcg
     6721 caggttcgcc agcaggcgga aaaactggtc agggagatgg cggcatgacg acgttaactc
     6781 aatgccagca gcaggtgctg gatatgctga tttcttatca gaaagaacgt ggcttcccgc
     6841 caaccaatca ggaggtggca accatgctgg gataccgttc ggtgaatgca gcggtggagc
     6901 atcttcgcgc actggagaaa aaaggcgtca tcacgataaa gcgtggcgtg gcccggggga
     6961 taacgcttca taccgcggtg aaggacgacg acagcgaggc ggtcgggatt atccgcgcac
     7021 tgcttgccgg tgaggaaaac gcaaggctgc gtgcaaccca ctggttacat gagagggacc
     7081 tgaaagtatg aagctgatcc tgccttttcc gcccagcgtg aacacgtact ggcgacaccc
     7141 caacaaaggg gcgtttgctg gtaagagcct gataagcgcg gcggggcgaa aattccagag
     7201 cgcggcgtgc gcagcaatag ttgagcagtt acgtcgtctg ccgaaaccaa cgtcggcacc
     7261 tgcttcagtg gagatcgtgt tgtttcctcc ggataaccgg atccgcgatc tggacaacta
     7321 taacaaggcg ctgtttgacg ccctgaccca cgcgggtgtg tgggaagacg acagtcaggt
     7381 gaaaagaatg ctggtggagt gggggccggt tatcccggaa gggaaggtcg agatcactat
     7441 caataagtac gagaaaacgg cgggtgcagc cgcctgatta agaggagaaa cgaagtatga
     7501 ataatctgat ggtcattgat ggtattgaag ttcgtcgtga tgcttatggg cgttacagcc
     7561 tgaacgatct gcacagggct gccggttctc aggataagca taagcctgca ttctggctcc
     7621 gcaatgagca aaccgaacgt ttaataagcg agttgcagat ttgcaactcg gtcaatatag
     7681 agccagttaa cgttagtcgt ggcggaaata accaggggac gtatgtctgc aaagaactgg
     7741 tgtatgccta tgcaatgtgg atcagcccgt cattccatct gaaggtgatc cgtactttcg
     7801 atatggtaac cagcgcaccg gaaaaattat ccgggcaggc tgctgacaag atgcaggctg
     7861 gcgtgattct gctggacttt atgcggcggg agttaaacct gtctaactca tctgtgcttg
     7921 gggcctgtca gaaactccag gaggctgttg gcttaccgaa tctggcacca cgctatgcca
     7981 ttgatgctcc tgctgacgcg cctgatggct caagtcgccc tacgctgtcg ctgagtgcac
     8041 tgctgaaaca gtatggtatc cgcctgacag ctaatcaggc atatcaccag atggcgaagc
     8101 tggggatcgt tgaacaacgc gaacgataca gccgtaccgc gattaacaac atcaaaaaat
     8161 tctggtcgct gacggcgaaa ggctgcatgt tcggcaagaa cattagcagt cctgcaaatc
     8221 cgcgcgagac gcagccgcat ttcttcgaat cccgattccc tgagctgtta aagctgctcg
     8281 ataccgttca ttgaggtgac cgtgagagca ctactgaccc ctgaaattgc cccgcgtatg
     8341 gggatcgtat tgttcagacc aggttcagag ctgatgcccc tgtttatgca ggggcgtgta
     8401 ctgctggagc ctgagccgga acgttattca tctttcgcca gtggtgccgt tcccgcggca
     8461 tcacaaccgc tggcggatga tcctgctgtt cgggccgtgt tccgcaatga ggcagtgatc
     8521 cgtcgtgctg gtggcgtgga atgtcttgaa agctggctac ttcgtgaaaa aggctgccag
     8581 tggcctcatt ccgactggca cagcgagaac atgaccacaa tgcgacacgc tccgggcgca
     8641 atccgtctgt gctggcactg tgataaccag ctgcgcgatc agttcacgga acggctggaa
     8701 tcaatggcaa cggataactg tgcccgctgg gtgttgtctg ttgtgcgtcg ggatctcggt
     8761 tttgatgata gtcacgttgt gacaatgccg gaactgtgct ggtggctgat tcgtaatgac
     8821 ctggcggatg ccttaccgga aagtgcagcc cgtaaggcac tgagattacc aaagcctgtt
     8881 gtgccgtctg tcacccggga aagtgacctt gtgccttcgg ttcctgccac cagcatcatc
     8941 caagataagg caaaaaaggt gctggcgctg aaagtggatc cggagtcgcc ggagtctttt
     9001 atgttacgcc caaaacgtcg ccgctgggtt aatgagaagt acacgcgctg ggttaagaca
     9061 cagccgtgtg catgttgtgg aaagcccgct gatgatcccc accacctgat aggccacggt
     9121 caggggggaa tgggtacaaa agcgcatgac ctctttgtgt tgcctttgtg cagaaagcat
     9181 cacgacgagc tgcatgcgga taccgtggca tttgaagaga agtatggctc ccagctggag
     9241 ctgatatttc gttttatcga tcgtgcgctg gcaattggcg tgctggcctg atttggtgga
     9301 gaaagttgat gcgtgatatt cagatggttc ttgagcgttg gggagcatgg gcggcgagtg
     9361 atagttcagg cgttgactat tcacctatag ctgctgggtt taagggactt cttccctata
     9421 ccagcaagaa acgcttggct tgttcggata gtgatgcatt aattattgaa ggttgtcttg
     9481 ctcgtctaaa gcaaaaaaga ccttatgagc attcgcttct tgtggcacat tatttataca
     9541 gaatatccaa gcgtaagatt gcaaaggcgc gtggaaagga tgagaaattg atacgtatag
     9601 aaatacagct agccgaagga tttattgatg gctgcctttc catgctagat ctaacattag
     9661 atttggacgt ttaataatac gccccatgca ggggcgtatt atttactgga tgaatgacat
     9721 ttgattaata tattttatca ataactctct gggtgtagta ctccaaaaat ttaggtgttc
     9781 ataatcagta taaacagtgg tgaatttttt aagttcttca tcacttttag gggcaaatcg
     9841 ctcattaaga gtaatactgt ctttaattat tccgaagaat acaatagata tttcaggaat
     9901 ttgcgatctt ctctcataag aataaggtat ttcttttatg ttaagcagat ctgctaaatg
     9961 agatatagat tctgcggtaa tttcttgttt tagtttttcc ttgtcaccaa agcaatgtat
    10021 gaagacagct acgacaaaga tcttatttat taaaggatca ctgcttttgt actcatcgtc
    10081 gtttaatagt tgaaaaatgt taagattgcg tgaaaaagtt tgtgtttcac gtaaggataa
    10141 gttggttcgt tgaattaaat cacggataaa tctaccagat aacctattaa tttcatttaa
    10201 tgattttgtc ttgtctacaa ggtaattcca atatataaca gatgttttac atacgacgtg
    10261 accatttatt aaacatgtgt ctggaagcgt gatggtatat tttataaact tgtcaagata
    10321 cttttgtgag ttaatactat aaccataaat atgatttatc gatgctttta attgttctgt
    10381 gtttgtaact aaaataaaaa agacattatt gatgtcaaaa atgtgtttta ttgtttcaat
    10441 gacatttgtt gaaaaactcg gcttacatcg gtctaattca tcaattataa ttactatctt
    10501 ttgactttct gaaatgcttt cgatgcaaga tttaagtgaa tttatgtttt tctctgattc
    10561 catatggtct tcaagcaaat tttcaatagt cccatctatt gctgcattgc ttgctttctt
    10621 catcgcatct tggaattctt cggaaacttc actagcctcc tgtcgtaaaa accaacctgc
    10681 accagctttt agtaccgttt ttaaaccaaa gcgaattgca ggaagagatc tcttaatgaa
    10741 gtgttgtttt tcttcctcag gtaaaatgct ggcaattgca gaggttatga gaagtaatgg
    10801 agattctgca tgatcccctt taaaggcatc aatataaaca actttagatt cagtttcttg
    10861 ctcaataatg agatttttca gtttgatact aaattctgtt ttccctgttc cccatgcgcc
    10921 gtctattacc agtggtgaaa tgtctgcctc tggttttagc aacttgatga tattttcagc
    10981 gatgtttctg cgttggaact cgtcacgttc agtgaaagat agtgtatcta acataatata
    11041 aacctattaa ctcctacagt aaaaaacgaa taatacgact ttgggattaa aaatcattaa
    11101 cgcggtccgc aaaaattctt gtaatctgtt aagagtggtt acttcgccac acagcttaaa
    11161 cccgccgtcg agcgggtttt gtcgtttttg ttgctgggga ttcgttgggc ctggcctatc
    11221 ccgcagttat ccattggttc ggctctttga cgtttccgct tctgatttgc ggtacatgat
    11281 gttccctcaa tttgcacctg ctgtatcagc gaggtgagag ataactacaa atgcctcata
    11341 acccaaatac ctggttggag ttggtccaga gctggtggcg tggagacaca ccgctgggcg
    11401 cagtgattat gtcgattgtt atggctggct tgcgcattgc atattttggc ggtggtgggg
    11461 gctggaaacg aaaaacgctc gagattttgc tctgtggtgc tctgacgctg acttttgcat
    11521 ccgctcttga gtatgtcgga tggcctaaat ctctttctgt tgccattggt ggcggcgttg
    11581 ggctaatcgg ggtcgatgct attcgtgggg ctgcaatgag agtaatcggt aataagtttg
    11641 gtggctctaa ggagtaattt atgcaggtac taaattccca gcgtaaagct ttcctcgata
    11701 tggtggcatg gtcagaagga acggataacg ggcgacaacc gacacgtaat cacggttatg
    11761 atgttattgt tggcggcgaa ctgtttactg attactctga tcaccctcgc aaacttgtca
    11821 cgctaaaccc caaactcaaa tcaacagccg ccggacgtta tcagcttctt tcacgctggt
    11881 gggatgccta ccgtaagcag cttgctctga aagacttctc ccccaaaagc caggatgctg
    11941 tggcactgca acagattaaa gagcgtggcg ctttaccgat gattgatcgc ggtgatattc
    12001 gtcaggctat cgaccgttgc agcaatatct gggcgtcgtt acccggtgca ggttatggtc
    12061 agtatgaaca taaaatcggt gatttgattg cccggtttaa agaggctggt ggggtggtaa
    12121 atgaagctga gctataagct gcttatcgtt ggtttcttcg ttactgtcat tggttctttc
    12181 atctggtcgg ccaaccacta ctacagcaaa tatcagtacg aaaagaaacg tgctgatgag
    12241 gctgtacgaa atgctgaatc agcaactgcc attaccaata acgtcctgca atcactacaa
    12301 atcgtcaata cagttctgga ggctaaccag catgcaaaac agcagatcgc actggagtca
    12361 cagagagccc gggaagatat caaagtggct gttgcggatg atgattgtgc ttcacgccct
    12421 gtgcctgatg tcgctgctga ccggttgcgg aagtacgcgg acagtatacg tgcaggttcc
    12481 aacgatgccg ttaccgacga acctgctcgc tgaaactcca cagccagtta tacccaatcc
    12541 tctgacttat ggggatagtc ttagtttgaa tgtaagtctg ctatcagcac tggggctatg
    12601 taaccgtgat aagtctgatc ttcgtaggtt aggagagcaa aagtacaatc tgcatttgaa
    12661 taataatatt cattaggtga aatattatta tttgactgtt ctagttatta tgcatttagt
    12721 tacaatactc tcactattaa cagtgaggta aaaatgaacg aaaattatat tgcatatgag
    12781 acacttgtag caaaccgtgc tgctgctgag tgggcttgtt gggcaatgat tgcatcttgg
    12841 gtgagtgctg gagctactat tgttactttg gttttggcgt tcaaggcatt gtttacgtgg
    12901 cgggaacaag agaaaacaaa agtaaaaata gattttagga atgcattaaa gaaattaaag
    12961 acagctctat tatttatgcc tgtcaatatt gaccccgaga aactcaatga tgaacgagag
    13021 caagttattg cgaaatggct atttaaagat gtagatctta ttagtcagca aattgagttg
    13081 ggagaagaga atgttaaaag atttgatgaa cttttgagta ttttcgattg ttgccagtct
    13141 tcatggtttg cgacagagca cttatttgat aatactgagt tagaaaaagt ttggcatgag
    13201 ttcgagtcta actttaataa atatataaat ggtggtgaga gtaaggattt acttatgaaa
    13261 atgcttgata agctcatctc ttctagattt gtatttgagt caagataatt gcctttgagt
    13321 atttttcttt attattttac ttattataaa ttttttatat gccccctaga atcccaaaag
    13381 cctgccgtgt tcgcggctgc cgctctacaa ccacggaccc gtcaggctac tgcgaaagcc
    13441 ataaaagcga aggctggaag caatacaagc caggacaatc tcgtcatcag cgcggttatg
    13501 gttcgaagtg ggatgttatc cgcgtgcgtg tcctgaagcg tgacaaaggc ctgtgtcagc
    13561 tatgtctgcg tgctggtgtg gtgcgtgagg cgaaaactgt tgaccacatc atccctaaag
    13621 cgcatggcgg cacagatgcc gacagcaatc tgcagagcct gtgctggccg tgccataagg
    13681 agaagacggc ccgtgaacgg ctaaagtgat cataattctc aactgtctga ggggaggggc
    13741 gggtcaaatc tctgtgacct gacgtcttcc ggactgcccg ccccatcgtt tttttatacc
    13801 cgcgaaaaat gaaatttaac caggagtgcc gcatatggct ggaacggcgg ggcgttccgg
    13861 gcgtcgccct aagccaacgg cgcgcaaggc gctggccgga aaccccggca agcgagccct
    13921 gaacaaagat gaacctgttt ttacgcccat caaaggtgtt gagccaccgg agtggttcgc
    13981 tgaagaagag cttcctctcg ccacgatcat gtggcaactg acaaccaaag aactctgcgg
    14041 tcagggcctg ctgtgcgtga ctgaccttgc ggtgcttgag cggtggtgtg tggcctatga
    14101 gttctggcgg cgtgccgtga aaaatattgc cagacagggc aacaccatca ccggtgcaat
    14161 gggcggtatg gtcaaaaatc cggagctgac cgccaaaaaa gaacaggagt ccgagatgag
    14221 cagcacgggg gcaatgctcg gactcgaccc cagtagccgc cagcgtctga ttggcctggc
    14281 ggggcagaag aaagccacta acccgtttct gaaaatcatc gagtcatgag ccggaaatct
    14341 taccccaacg taaatgctgc caatcagtat gcccgtgatg tcgtgcgcgg aaagattgtg
    14401 gcctgccagt ttgtgattca ggcctgccag cgccatcttg atgacctgat ggcggaaaaa
    14461 agtaagtcgt ttcgttaccg cttcgacaag gagctggctg aacgggccgc gaaatttatt
    14521 cagctgttgc cacacaccaa gggggagtgg gcattcaaga ggatgcccat cacgctggag
    14581 ccgtggcagc tctttgtgat ctgctgtgcg tttggctggg tcaataaagg gtcccggctg
    14641 cgccgcttcc gggaggtgta taccgaaatc ccccgtaaga acggcaaatc ggcaatctct
    14701 gccggtgttg ccctgtattg ttttgcctgt gataacgagt ttggcgcgga agtgtattcc
    14761 ggtgccacga cagagaaaca gacgtgggaa gtctttcgcc cggcgcgact gatgtgtaaa
    14821 cgcacaccca tgctgacgga agcgttcggg attgaggtta acgcctcaaa catgaaccgt
    14881 ccggaggatg gggcgcggtt tgaaccgctg attggtaacc ccggtgatgg ttcatcaccc
    14941 cactgtgcgg tggtggatga atatcacgag cacgccacag atgcgcttta caccacgatg
    15001 cttaccggga tgggggcgcg acgtcagcca ctgatgtggg ctatcactac cgccgggtac
    15061 aacattgagg ggccgtgcta cgacaaacgg cgggaagtca tcgagatgct caacggttcg
    15121 gtacccaacg atgaactgtt cgggattatc tataccgttg atgaaggtga cgactggacc
    15181 gacccgcagg tgctggaaaa agccaatcca aatattggcg tgtcggttta tcgcgaattt
    15241 ttgttaagtc agcagcagcg tgcgaaaaat aacgcccgtc tggcaaacgt ctttaaaaca
    15301 aaacacctca atatctgggt gtcggcgcgt tcggcgtatt tcaacctggt gagctggcag
    15361 agctgcgagg ataaatcact gacccttgag cagttcgagg ggcagccgtg cattctggcc
    15421 tttgacctgg cgcgtaagct ggatatgaac agcatggcgc gactttatac ccgcgagatt
    15481 gacggtaaaa cgcattacta cagtgtggcc ccgcgtttct gggtaccgta tgacacggtg
    15541 tacagcgtcg agaaaaatga agatcgacgg acagccgaac gctttcagaa atgggtggaa
    15601 atgggcgttc tgaccgttac cgatggtgcg gaggtggatt atcgctacat cctcgaggag
    15661 gccaaagcgg cgaacaaaat cagtccggtc agtgagtcac ccatcgaccc cttcggggcg
    15721 accgggttgt cacatgacct tgctgatgaa gacctgaacc ccatcactat cattcagaac
    15781 tacaccaaca tgtccgaccc gatgaaagag ctggaagcgg caattgaatc ggggcgcttt
    15841 catcatgatg gcaatcccat catgacctag tgtatcggca acgtggtcgg caaaaccatt
    15901 ccgggtaacg atgatgtggt gaagcccgtc aaagagcagg cggaaaacaa aattgacggt
    15961 gcagttgcgc tgattatggc ggttggcaga gccatgctgt acgagaaaga agacacgctg
    16021 tctgaccaca ttgagtccta tgggatccgc tcgctttaac tgaggtaatt atgatcatgc
    16081 tgattctcgc gcctctggtg ggcgtgctgg gggcgctttt gctggcgtat ggtgcctggc
    16141 tgatttatcc cccggcgggg tttgttgttg ccggggcgtt gtgcctgttc tggtcgtggc
    16201 tggtggcgcg atatctcgac cgtacacagt cgtctgtcgg cggaggtaaa tagtgttctt
    16261 ttcgggatta tttcaacgaa aaagtgacgc accggtgacc acgccagcag agctggcgga
    16321 tgatatcggg ttgtcctacg acacctatac cggaaagcag atcagcagcc agcgggccat
    16381 gcgactgacg gcggtttttt cctgtgtcag ggtgctggcg gagtcggtcg ggatgttgcc
    16441 ctgcaacctg tatcacctga acggcagcct gaagcagaga gccactggcg aacgtctgca
    16501 taagctgatc tccacgcatc ccaatggcta tatgacgccg caggagttct gggagctggt
    16561 ggtcacctgt ctgtgcctgc ggggaaactt ttacgcctac aaagtgaaag catttggcga
    16621 agtggctgaa ctgctgcccg tcgatcccag ctgtgtggta ccgaagctta acagtagctg
    16681 ggagccggtc tatcaggtca cattcccgga tggctccgcg gatgtactga gccaggagga
    16741 tatctggcat gtgcgcacgc tgacgctgga cggactggtg gggctgaatc ccatcgccta
    16801 tgcccgcgag gcaatatcgc tggcggcagc gaccgaagag cacggggcca gactgttcag
    16861 caatggcgcg gtgacgtcgg gtgtgttgcg tacagagcag acgctgtcag atcaggctta
    16921 cgagcgcctg aagaaagatt ttgaggagcg tcacaccggg cttggcaatg ctcaccgccc
    16981 gatgatcctt gagatggggc tggactggaa gtcgatggcg ctgaacgccg aggacagcca
    17041 gttcctggaa acccgcaagt ttcagcttga agaaatctgt cgtctgttcc gggtgccgtt
    17101 gcacatggtg cagaacaccg atcgcgccac tttcaacaat attgaagagc tggggctggg
    17161 atttatcaac tattcactgg tgccgtatct gacccgcatc gaacagcgga tcaacaccgg
    17221 actggtacga aaaagtaagc agggcgttta ttacgccaaa tttaacgccg gggcgttact
    17281 gcgcggggat atgaagtccc gttttgaagc ctacgccacc gggattaact ggggaattta
    17341 ctctcccaat gactgccgcg acctggaaga tatgaatccg cgtcccggtg gtgatgtcta
    17401 tctcacaccg atgaacatga ccacgaaacc ctccgatggc agtaaagccg gtaagcagaa
    17461 ggataacgcc aatgcagacg aaacaacgtc ttgatgtacc gctgagtctg aaatctgtca
    17521 gtgactccgg tgagtttgaa gggtatggct ccgtctttgg tgtaaaggac agccacgatg
    17581 atgtggtgat gtccggggca tttgccgctt ccctgcgggc gtggagtgac agaaaagcgt
    17641 tacctgcgct gctctggcag caccgcatgg atgagcccat cggtgtttac accgaaatga
    17701 aggaagacga tgtcgggctt tacgtcaggg ggcggttgct cattgatgat gatcccctgg
    17761 caaaacgcgc acatgcacac atgaaggccg gttcgttaac cggcctttct attgggtacg
    17821 tcctgaagga ctgggaatac gaccggacga aagaagcctt tctgctgaaa gaaatcgacc
    17881 tctgggaagt cagtctggtg acgtttccgt ctaacgacga ggcgcggatc agcgacgtca
    17941 agaacgcgct ggcccgcggg gaaatccccg aacagaaaaa aatcgaaaga gtcctgcgtg
    18001 atgtcggact ctcccgtacc caagccaaag cattcatggc cgggggctat atcgcactgt
    18061 ccctgcgcga cgctgaggat gtgggctctg cactgaatgc actgaaaaat ctgaacttct
    18121 aatcaggaga aatacgatgg cggtagatat taaagatgtc gaacaggtcg cgcaggagct
    18181 gcagcagaag tttgacgact tcaaagcaaa gaacgacaag cgcgtggatg cgattgagca
    18241 ggaaaaaggc aagcttgccg ggcaggtgga aaccctgaac gggaaactca gcgagctgga
    18301 aaatctcaaa agcgatcttg aaaaagagct gcttgagctg aaacgtccgg caggtggcgc
    18361 gcaaaataaa ctggccaccg agcataaaga agcgtttgtg ggctttttgc gtaaaggccg
    18421 tgaagatggt ctgcgcgatc tggagcgtaa ggcattgcag gtgggcaccg atgaagacgg
    18481 tggctatgcc gtgccggaag cgctggatcg caacattctc accctgctga aagatgaagt
    18541 ggtgatgcgc caggaagcca cggtgatcac cgttggcggt tccgactaca aaaaactggt
    18601 gaatctgggc ggcacggctt ccggatgggt gggggaaacg gatacgcgat cccagactgc
    18661 cacctccaga ctggagctga ttgaacctct catgggggaa atctacggca acccgcaggc
    18721 cacccagaaa atgctggacg atgccttctt caacgtggag gcctggatca acagcgagct
    18781 ggcaaccgaa tttgccgaac aggaagaaat tgcctttacc accggcgatg gtaccaagaa
    18841 gccgaaaggg ttcctggcgt atgaatccac tgatgaaacc gataaggtcc gggcgttcgg
    18901 caaacttcag catattgtat ccggcgaagc gacggcggtg accgcagacg ccattatcaa
    18961 actgatttac acgctgcgta aggcacaccg caccggcgcg aagttcatga tgaacaacaa
    19021 cagcctgttt gccatccgtc tgcttaaaga cagcgagggt aactatctgt ggcgtccggg
    19081 gctggaactg gggcagccgt cctctctggc gggttacggt atcgctgaaa acgaacagat
    19141 gccggatatc gccgctgatg cgaaagccat tgcatttggt aacttcaaac ggggttacac
    19201 catcgttgac cgtatcggca cccgcattct gcgtgacccg tacaccaata aaccgtttgt
    19261 cggtttttat accaccaagc gcaccggcgg gatgctggtc gattcgcagg ccatcaaact
    19321 gctgaagatt gcagcggcgt aatcactcag gggcgcggaa ccgcgccccc tgttctgacg
    19381 ggtgaagaat catgatcctg aaacaagatc tgaaatggtc accggacggt atgcgtgttg
    19441 aggtcattcg ggccggtgag tatgacgacg gggcgcttcc tgcccgggtg caggagattg
    19501 cacttcaggc cgggttagca gagcgcggaa ccagtgcaaa aagcagtaaa gcgacaaaag
    19561 agaaaaaagc cacgaccagt aaagagggct gagtatgctt ctgacaatgg aagagattaa
    19621 agcccaactc cggctggatg aggatttcga tgctgatgac cgccatctgc aactgctggc
    19681 ctgtgcggca caaaagcgga cggaaacgta tctgaaccgg aagctctatg caccggatga
    19741 aaccattccg gacagcgatc cggacgggct gcacctgccg gatgatattc gtctggggat
    19801 gctgatgctt atcagccatt tttacgaaaa ccgctcgtcg gttacggaag tggagaaact
    19861 tgacatgccg cagagttttg gctggcttgt cggcccgtac aggtactttc cgcaatgaaa
    19921 attcgtcagg cgcagaccag cgcaacctac attctgccgg accccggtga actgaataaa
    19981 cgcgtcctga tccgcctgcg ggtggatatg cccgcggata actttggcgt ggagcctcaa
    20041 tacctgatta cgttccggac atgggcgaag gttatccaga ccagtgccac cacctggcag
    20101 gaaaccgcgc agaccgggga cgccatcacc cattacatca ccattcgtta ccgccggggg
    20161 attaccgctg attatgaggt ggtctgcggt gacagtgtgt accgggtgaa acgtcagcgc
    20221 gatctgaacg gggcgcggcg ctttctgctg ctggagtgta cggaactggg cgaatgtggg
    20281 cagagtcacg gaggcaacaa tgacgacttc ctttttgcac gttgattttc agcagcccgc
    20341 ggagatgcgc tttaaccgcg cccgtgtccg gcgggcgttt gtcacgattg gtcagcgtca
    20401 tatgcgtgat gcccgtcggc tggtgatgcg ccgtgcgcgg tcggcaccgg gtgaaaaccc
    20461 cggttatcag accggacgcc tggctcgttc gattggttac atggtaccca gagccagtaa
    20521 acatcgccct ggttttatgg cacgtatagc ccctaaccag cgtaatggag agggaaaccg
    20581 ccgtatcacc ggtgattttt atccggcttt tttgttctat ggcgtgaggc gaggggcaaa
    20641 gcgtcgtcgc agccatcatc gtggtgcatc cggtggcagc ggctggcgac tggctccacg
    20701 taataacttt atggtggaaa cgcttgaaaa gaaccgcagc tggacacgct attttctggc
    20761 gcgggaattg cgtaaatcac tgaagccgga gcgacgacac agatgaaact gacgcctgtt
    20821 attgctgcgc tgcgtgcccg ctgcccgtat tttgaaaacc gggtggcagg cgcggcacag
    20881 ttcaaaaatc tgccggaggt cggaaagctg agactcccgg cggcgtatgt ggtaccgggt
    20941 gatgactctc cgggagaaaa caaaagccag accgactact ggcaggagct gaaagagggc
    21001 ttctccgtgg ttgtcatact gagtaacggg cgtgatgagc gcggtcagtt tgcctcgtat
    21061 gatgtggtgg acgatgtccg gcaaatgctc tttaaggccc tgctgggctg gaacccggaa
    21121 gcgtgcggta acccgattac ctatgacggc ggcacgctgc tggatctgaa tcgtcatgag
    21181 ctgatttatc agttcgattt ttcggtcatc agcgagttga ccgaagacga tacccgccag
    21241 caggatgacc tgaacagtct ggatgaactg cgaacgctgg cgattgatgt tgattatcta
    21301 gagcccggta acgggcctga cggcgatatc gaacatcaca ccgaaataac ccttccttcc
    21361 tgagaatctt catgtttgtg aaacctgtta aagggcggtc agttcctgac cctgcccgcg
    21421 gagacctttt gcccgccgag gggcgaaatg ttgatgagaa caactactgg ctgcgccgtg
    21481 aagcagcggg tgatatccgg cgcgtgaatg aaaaggtgaa taccgatgac gagctttaac
    21541 accattccgt caaatacgct ggttccgatt ttttatgcgg aaatggataa ctcggcggcg
    21601 aatactgcac aggacagcgg agcatcgctg ctgattggtc atgccaataa cggtgcagag
    21661 attgttgcca acagtctggt gctgatgcca tcggcagact atgcacgcca gatttgtggt
    21721 gcgggaagtc agctggcgcg tatggtcgag gcttatcgcc agaccgaccc gtttggtgag
    21781 ctgtatgtga ttgccgttcc agaagccaca ggcgcggcgg caacggttac gctgacggtg
    21841 accggggaag caaccgaaag cggcacggtg aatgtctatg tgggacgtac ccgcgtgcag
    21901 gctccggtga ccaacggcga taacgtcacg acgattgcca gcagtatcca ggatgccatc
    21961 aatgccgttc cggccctgcc gtttacggct tcatcttcgg caggcgtggt cacactgacc
    22021 gcgcgtcata aggggctttg cgggaatgaa attcctgtca gcctcaatta ctacggcttt
    22081 ggtgggggcg aagtgctgcc agcgggcgta cagattgccg tggcgacggg taccgccgga
    22141 acgggtgctc cggttctcac cggcgcggtg gctgcaatgg cggatgagcc gtttgattat
    22201 atcggcctgc cgttcaacga cacggcctcc gttaacacgc tggtgaccga gatgaacgat
    22261 accagcggtc gctggagcta tgcgcgtcag ctgtatggtc atgtgtatac ggcaaagatc
    22321 ggcacgctgt cagaactggt gaccgcaggt gaccagttta accagcagca cattaccctg
    22381 gcgggatacg aaaaagagac ccagacgcct gccgacgagc tggcggcaag ccgtaccgcc
    22441 cgcgcagcgg tgtttattcg caacgatccg gcacgtccca cgcagaccgg tgagctggtg
    22501 ggtatgctgc ctgcgccgaa ggggaaacgg ttcacgatga ccgaacaaca gaccctgctg
    22561 tctcatggcg tggcaacggc gtatgtcgaa agcggggtgc tgcgcattca gcgtgatgtc
    22621 accacgtaca ggaaaaacgc ttacggtgtt gcggataaca gctacctcga cagcgagacg
    22681 ctgcatacca gcgcgtatgt gctgcgcaaa ctgaaatccg tcattaccag taagtacggg
    22741 cgtcacaagc ttgccagcga cggtacccgc tttggtcccg gtcaggcgat tgtcacaccg
    22801 gcggtgatca aaggggaact gctggcaacc taccgtcagc ttgagcgtgc ggggatcgtg
    22861 gaaaactacg aactgtttaa gcagtacctg gttgtggagc gtgatgccag cgatccgaac
    22921 cgcctgaaca cgctgttccc gcctgactat gttaaccagt tgcgtgtctt tgccgtggtt
    22981 aaccagttcc gtcttcagta ttcagaggag tctgcataat ggcccgtatc gggggaacct
    23041 gttatttcaa aattgacggt caacagctat cgctgaccgg cggcattgag gtgcccatga
    23101 acaggacggt caatgatgac atcatcggcc tggacggttc agtggaccgc aaggaaactc
    23161 accgtgcgcc ttatgtcaaa gggaccttca aggtgccgaa gaattttccg gcgagcaaaa
    23221 tcacctcgtc tgatgagatg accatcactg ccgagctggc gaacggtcag gtctatgtac
    23281 tgtcgtctgc ctggctgcac ggcgaagcga accataatgc cgaagaaggc acggtcgatc
    23341 ttgagttcca cggtgaagaa ggggattacc agtaatgaaa gagcttgagt taaagaaacc
    23401 gattaccgct catggcgaga cactctccgt actggagttt gatgagccca ccgggaaaga
    23461 tgtccgcgag ctggggtatc cctaccagat gaatcaggat gagtccgtca gacttctggc
    23521 gcatgtggta tcgaaatata ttgtgcggct ggcgaaagtg ccgcaaagct ctgtcgacca
    23581 gatgtctccg gcagacctga atgcagcggc gtggcttgtg gctggttttt tcctccaggc
    23641 ctgacggctg aataccttac tgatcgcttc tttgattgcg ccagctactg gcgcattaat
    23701 cccttcgaat tgctgaatat gccgatcagt gaaattccct tgctggtcag tcaggcaaac
    23761 aggatagagc aggagaaacg cacacatggc tgaatttgag cttaaggcgt tgatcaccgg
    23821 tgtcgacagg ctttctcccg cgctgtcgaa aatgcaaaag aaaatccggg gatttaaacg
    23881 ccaggcggaa gaagcgtcac agggtgggct ggcgcttggt ggcggactgg cagcgggtct
    23941 gacgctttcc ctgaaatctt atgccgatca ggaaaacgcc gccaccgggc tgaaagtcgc
    24001 catgatggat gcgaatggcg aggttggaaa gagctttcag gacatcaata aactggctat
    24061 tggcctgggt aaccagctac ccggtacaac ggctgatttc cagaacatga tgcagatgct
    24121 ggtgcgtcag gggatcccgg cagaaaatat tcttggcggt gtgggtaaag cgacagctta
    24181 tcttgcggta caactgaaaa aaacaccgga agcggctgct gagtttgctg caaagatgca
    24241 ggatgctacc ggaacggcgt cagaagacat gatggggctg ttcgacacta tccagaaggc
    24301 gttttatctg ggcgttgacg ataccaacat gttgtccttc ttcactaaaa ccagttctgt
    24361 tctgaagatg gtgaacaagg acggtcttca ggctgcacag agccttgccc ccatcagcgt
    24421 catgatggat cagatgggga tgaacgggga gtcggcaggt aatgccctgc gaaaagttat
    24481 ccagtccgga ttaagcgtta agaaaatcag ggacgtcaat aaaatcatgg cccgccagaa
    24541 actcggggta cagctcgatt ttactgacgg caaaggaagt tttggcggtc ttgataacat
    24601 gttcaggcaa ctggcaaagc tgcgaaaact gaccgacgtt aagcgaacag gtgtacttaa
    24661 ggcaatattt ggtgatgatg ccgaaaccct tcaggtggtc aatgcactaa tcgataaagg
    24721 aaaggatggc tacgatcaga tccagcagaa gatgaataaa caggccagcc tgaataaacg
    24781 tgttcaggcc cagcttggta cgctgtccaa cctgtgggag gcaatgacgg ggaccgcaac
    24841 taacggcctt gcggctattg gcggcgcatt ttctggtgac gccaaaaata tcacgcaatg
    24901 gctgggggag ttgggggaga aattcacgaa gtttgcggat gaaaatcccc gggttattcg
    24961 cggcgtcgtc gggcttgctg ccggtcttgc gattctgaaa ctgggattga tgggcgtggg
    25021 cagtgccatc agtatcgtca gcaggatcat gtcgatgacg ccgattggca tgattgcgac
    25081 ggcgattgcc ctggctgcgg gattaattat cactaactgg gatgttgtcg gaccttattt
    25141 taagaaactc tgggaaacca ttagtcctta ttttgaggct ggctgggaac ttctgaagaa
    25201 ggtttttgcc tggtcgccgc tggggatggt gatcaataac tggggaccgg ttgttaagtg
    25261 gtttcaggat atgtgggata agctgaagcc aattattgag tggtttaccg acagttcagg
    25321 tgacacggtc gatgccatta actcggcgca gtggggcgcg ggtgcttatg atgcttatgg
    25381 gacgggaata ccggcgcggg gatacatacc ttatccggcg gtggatctgg ctcagtcaaa
    25441 caacgcctcc gatgccacag gcccgaatcc cttcatgatt aacaaagctt ctgcgccaaa
    25501 agttgatggt gagatcaagg tctcttttgt gaattcgcct ccgggtatgc gggttatgga
    25561 aacgcgatcc agcggttttg atgtcagcca tgatgttggc tatacgcgct ttggcaggta
    25621 atgaaaaatt aatctgttaa tgagtcccac tccggtggga ttttttatgt acggagttta
    25681 tatgacgtgg aaagacagac ttcaggacgc gtcatttcgc ggtgtgccgt ttaaggttga
    25741 agaagaaaat gcgggaaccg gtcgtcgtgt ggaaacgcac gaatacccga accgcgacaa
    25801 accctatacc gaagatctgg gaaaagtcac tttccgcccg tccatcacgg cttatgtggt
    25861 gggagatgac tgctttgacc agcgcgatcg cctgattgac gcgctgaata aacccggtcc
    25921 cggcacgctt gtccatccga catacggtga gctgaaagtc tgtgttgacg gggaggttcg
    25981 ggtcagcaca tcgaagggtg aagggcgtat tgtccgcttt gacctgaagt ttgtcgaagc
    26041 gggagaactc tcttacccca catcaggtgc ggcgacggcg cagacgctga tgtcatcctg
    26101 ttctgcactg gatgactgca tcagtgacag tttcagtggt ttcagtatcg atggcgtggc
    26161 agattttgtg cagaacgacg tcgtcggtaa tgccagcaca atgcttgggt atgtttctga
    26221 tgcgatgaaa gtggtggatt ctgccgtatc ggatgtcgcc aggctgttgc agggggatat
    26281 ctcggtactt ctgccgccgc catcgtcagg caaaaatttc gttgagcagg tgcagaaaat
    26341 gtggcgtacc gggaaacgcc tttatggtaa cgccagcgac ctggtcacca tgatcaaaac
    26401 gctttccggt gtcagcctcg gcagcgatct gcaaccgcgc ggcgtctgga aaacggacag
    26461 taaaaccacc gccacggcga cgcagcagcg taacgtggtt gccagcaccc ttcgtacgac
    26521 cgcaatcagc gaagcggcgt atgccgtcac ccgattgcct gcgccaacaa cttccgcggt
    26581 gatgcagaat gccgcagtgg agcaggcaac aacacctgcg cagagcactg gctggccttc
    26641 cgtcacgcat ccggcactga acaatgcacc ggcggtgaaa aacacggttg acctgccgac
    26701 gtgggaagaa ctgactgaca ttcgcgacac actgaatacg gcaattgata aggagttgtc
    26761 ccgtacaacc agtgatgcgc tgtttctggc gctgcgccgg gtgaaagcag atctgaatgc
    26821 ggatatcaac acgcgccttg aacagtctgc acggatcatt cagcgcacac cggatgaggt
    26881 tttacccgcg ctggtgctgg cggcgacctg gtttgataac gcggcgcgtg acgcggacat
    26941 tatccggcgt aatgccatta cgcatcccgg ctttgtgccg gtgatccctc tgaaggtgcc
    27001 agtgcaatga acgataacgt cacgctacgg gtaaatggcc gggagtggaa tggctggaca
    27061 tcggtgcgca tcggtgccgg tattgaacgg ctggcgcggg atttcagtgt ggagatctcc
    27121 cgccagtggc cgggagatgg gggtatcacc acgcttcagc cgcgcattaa aaacggttca
    27181 aaagtggaag tgctgattgg tgatgagctg gtgatcaccg gctgggtgga ggcgacgccc
    27241 gttcgttacg atgcccgttc ggtcagcacc ggtattgccg gacgcagtct gacggctgac
    27301 ctgattgact gtgcagccga accgacacag tttaacggac gagagctggt acagattgcg
    27361 caggcgcttg ctgcgccttt cggcattgag gtggtgaaca acggtgcgcc gtcgggtgtt
    27421 attcctgacg tccagcccga tcacggtgaa acggtgattg aggtaatcaa caaaatactc
    27481 ggtcagcagc aggcactggc ttacgacgac ccgcacggca ggctggtgat tggtggtatt
    27541 ggctcaacgc gggcacatac tgcgctggta ctcggggaaa acatcctttc ctgcgatacg
    27601 gagaagagta tccgggagcg gttttctgtt taccaggtgg cggggcagcg tgccggaaac
    27661 gacgatgatt tcggtgaggc caccaccacc gcgctgcggg cccgcacaga ggacgcattt
    27721 attgcccgtt accgtccgat gtatatcagg cagacagggc aggccacggg ggcaggctgt
    27781 attgcgcgtg ctgactttga agcccggcaa cgggcggcgc ggacggatga aaccacctat
    27841 gtggtgcagg gctggcgaca gggtaacggt acgctgtggc agcccaacca gcgggtgatt
    27901 gtcttcgatc cggtctgtgg ttttgacaat accgaactgc ttgtctcgga agtcacgttt
    27961 actcaggacc agaatggcac tctgacggaa atccgtgtcg gcccgcctga tgcttatctg
    28021 cctgaacccg aagaccccgg cgcgcggaaa aagaaaaaag ccagagtaca ggaggacccg
    28081 ttctgatgag gacgattgaa gccatgcagc gacaactcct cggcctgatt gggcgggccg
    28141 tggtgaaaag catcagtgcc gccacgaaat gtctgaccgt ggatgtgtcc ctgattgccg
    28201 gtgaacccaa agccggggtt gaacatcttg aaccctacgg ttttaccgca agggcaaaca
    28261 gcggtgcgga agcggtggtg ttgtttccgg atggcgaccg ttctcatgcg gtggttgtta
    28321 cggtgtcaga ccggcgctac cgcctgaaag ggctgcagac gggtgaggtg gctgtctatg
    28381 acgatcaggg gcagtccgtg acgctgaccc gggaggggat cgtggtggac ggtgcaggta
    28441 aaacgatcac gtttcgcaat gcacctgaag cacgttttga aatggacctg gaagtaacag
    28501 gacaggtgaa agacctgtgc gactccggcg gcaccaccat gtcagcaatg cggcttgcct
    28561 ataacggtca tcgtcacaga gagaacggtc agggcagtaa caccgataaa cctgataaag
    28621 ctatggaggc atgatggaac tgtggctgac ggtgaacggt aaacgcacct gcgccagcgc
    28681 accgctggat ccgctgaccc gcgccgtggt gatttccctg tttacctggc ggcgggcgga
    28741 gcctgatgac aacgccgacg tcccgatggg atggtggggg gatacctggc ctgcggtaca
    28801 gaatgaccgt tacgcctcca gactgtggct gcttcagcgc agcaaactga ccaatcagct
    28861 ggtgcagacg gtaagggggt atatccgcga atgcctgcaa tggatgactg atgacggcgt
    28921 ggtgtcccgt attgatctgg atatccgccg caccgggatt aatgaactgg gtaacagtat
    28981 cactctctgg cgtcgtgacg gaccggtaat gatttctttt gatgatctgt ggagtgcgat
    29041 aacgcatggc ggacagtgaa tttcagcgcc cgacgctggc agaaaatatc agtatgctcc
    29101 gtaacgattt attcgccagg ctggacgtca gcgacacgct ccggcgcatg gatgaagacg
    29161 tgcgggcaaa ggtgtatgcg gcggcgctgc atacggttta cgggtacatc gattatctgg
    29221 caatgaacat gctgcctgac ctgtgcgatg agttctggct ggcgcgacat gctgcgatga
    29281 aacggtgtcc gcgcaagggg gccacggctg ccagcgggta tatgcgctgg gaaggtgtca
    29341 gcgatggcct gaaggtgacc gccggaagtg ttattcagcg cgatgacctg gttcagtaca
    29401 cggcaactgc cgatgcaacc agctccggtg gtgtcctgcg cctgccgatc gcctgttcaa
    29461 ctgcaggcgc ggtcggtaac gctgacgacg gtacggcatt aatcctggtc acgccggtga
    29521 atggtctgcc gtcttccggt gtggcagata ccctgacagg tggatttgat actgaagatc
    29581 tggaaacgtg gcgcgcccgc gtcattgagc ggtattactg gacgccgcag ggcggggctg
    29641 acggggacta tgtcgtctgg gctaaagaag tgcccggcat tacccgcgca tgggcatacc
    29701 gtcactggat gggaacgggg actgtcggtg tgatgattgc cagtagtgac ctgattaatc
    29761 ccattccgga agaatcaacg gaaacggcgg caagacaaca tatcgggccg ctggccccgg
    29821 tggcaggctc tgatttgtat gtgttcaggc cggtggcgca tacggtggat tttcatatcc
    29881 gtgtgacgcc ggatacaccg gaaatacggg ctgccattac cgcggagttg cgttcgttcc
    29941 tgctgcgtga tggttatccg cagggagaac tgaaggtatc gcgtatcagt gaggcgattt
    30001 ccggtgcgga cggggaatac agccatcagt tgcttgcacc ggtggacaat atctccattg
    30061 cgaaaaacga actggcggta ctggggacga tttcatggac gtgacaaacg atgattacat
    30121 ccgcctgtta tcggcactgt tgccgcccgg tccggcgtgg tcatctagcg atccggcgat
    30181 tgccggtgcg gcaccgtcat taacccgtgt tcatcagcgt gcggatgccc tgatgcggga
    30241 gctggatccg cgcaccacca ctgaactgat aaaccgctgg gagcgtctgt gcggtctgcc
    30301 ggatgaatgt attcccgcag gaatacagac ccttcgccag cgtcagcaac gactggatgc
    30361 gaaggttaac ctggcgggcg gcatcaatga ggatttttac cttgcacagc ttgctgccct
    30421 gggcagacaa gacgccacca tcacgcgata cgacaaaagc acgttcacct gctcatcggc
    30481 ctgtactgac gcggtgaatg cgccggaatg gcggtattac tggcaggtca acatgccagc
    30541 cgccaccaac agcacctgga tgacatgtgg cgatccctgt gattccgcac tgcgtatctg
    30601 gggcgacacc gtcgttgaat gtgtgcttaa caaactctgc ccttcgcata cctacgtaat
    30661 ttttaaatat ccggagtaat ccatgcatcg tatagacacg aaaaccgcgc agaaggataa
    30721 gttcggcgcg ggtaagaacg gttttacccg tggtaacccc cagaccggca cacctgccac
    30781 cgatctggat gatgactact ttgacatgtt gcaggaggag ctttgcagcg tggtggaggc
    30841 atccggtgcc agcctggaga aggggcggca cgatcagttg cttaccgcac ttcgcgcgct
    30901 gctgttaagc cgcaagaatc cgtttggcga tatcaaatcg gatggcacgg tgaaaacggc
    30961 tctcgaaaac cttggtttgg gagaagcagc taaaaggaat gtaggtacag gggcgaatca
    31021 gatacctgat atgagcctgt tcgcgtcaat taataccgta acggctgctg cgcaaaaatt
    31081 tccgtctgga ttaattttac agtgcggtca gttgaatggt gccccgaatg tatcttcaac
    31141 atacgggatg aggttcccga tgacgttctc aagagtcatt gctgtcgtag ttacattgaa
    31201 cgttactggc gcggcagggc agccgactgt atcggcgaca agtgtccaga acactggatt
    31261 taatattacg gtgtcgcccg gttcaggata cggttcatct gctgatgcgt attacattgc
    31321 aatgggatat taacaaaatg tcatattttt attctgcatc gacaaacgga ttttatttga
    31381 ctgaatttca cggcaccaat attcctgatg atgcagtgga aatctcggaa tcagagtgga
    31441 aaacactgat taatgcacag agtgtaacaa aaatgattac ctgtggtgag aacggtcatc
    31501 ctgtcattgt tgaccgtcct tctccaacac cagaacaatt agccttaata aatgatgaaa
    31561 agaaatctgc actgatagca gaggcaacga atgtaatagc tccgcttcag gatgcggttg
    31621 atttaggtat ggcaacagat gatgaaacga aactgttact ggcatggaaa aaatatcggg
    31681 tgctgcttat gcgtgttaat gtagtaaaac ccgagtggcc tatgcatcca aataaataga
    31741 cttgttaatc taggataatt gttggaataa tactcatttt tcagtaggtt caggtagcct
    31801 gggtataatg ctcacttcta caatgatttc aacttatttt atttaattga tattacgaaa
    31861 cttaactcat acataaacaa tatgactcaa tactgtccta aatagctgcg cagaaaagtg
    31921 ctccactttc gctcagccct tgctgggcga agcatcagga aggcgttctc agctaggttg
    31981 tgttatatct ggcttgagtt ttgttgccca tacgcaacac attacccatc tgtaaacgac
    32041 aagtgtgaca gaattaggtg cagctattta gactatatcg acgcagtaac gataatagat
    32101 atcgaaactg tactagatat tagctactta tggttaccgg gggaagtctt ttgagcactg
    32161 gtgttatcaa tcggcttcaa ataattcacc aataaattat attgcgatag ctcatcacct
    32221 tcctggttgt ttatagccag tttatcagcg tcaccaccta ctgtttatgc ttttatttct
    32281 ttgattaatg agtatatgat attttaattc atctggtgta tttctttctc atgtgttggc
    32341 cgactgaata tctgttcagt gaaggggtaa ttttatgttt gcacttcgaa cattcccttc
    32401 agttgccaag aaagaaaacc gtaacatttg cgcattaatt ttaacagtat aatctgagca
    32461 taaatatgcg ctatatatct ataaaaaggc gctattaatt gcgccttttt atcattttat
    32521 caaaaatggc ccaccgactg tcctcgatag ccaatggcca attttatctg atacatatac
    32581 ataaatacag gtatatatga ttgcgcagat aggatttgga atggtcacgc tgaagcctat
    32641 gattgctaaa gccataggga taaataattt ggtgatatac tccattccac atatgttaag
    32701 agtatttctt ccagctctta caataaagtc attcccacaa attatttttg ctataaaaat
    32761 attaaatatt atcaa
//
LOCUS       DAJTXY010000033        32318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTXY010000033 DAJTXY010000000
VERSION     DAJTXY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.033"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO566_001662"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO566_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6604982.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNKFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO566_001663"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO566_001663"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6604983.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO566_001664"
     CDS             complement(3736..4092)
                     /locus_tag="OO566_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6604984.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO566_001665"
     CDS             complement(4096..4512)
                     /locus_tag="OO566_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6604985.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO566_001666"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO566_001666"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6604986.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5960
                     /locus_tag="OO566_001667"
                     /pseudo
     CDS             5738..5960
                     /locus_tag="OO566_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="OO566_001668"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="OO566_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6604987.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="OO566_001669"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="OO566_001669"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6604988.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="OO566_001670"
     CDS             complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="OO566_001670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6604989.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="OO566_001671"
     CDS             10540..11523
                     /locus_tag="OO566_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6604990.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQPAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="OO566_001672"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="OO566_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6604991.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="OO566_001673"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="OO566_001673"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6604992.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13465..14450)
                     /locus_tag="OO566_001674"
                     /pseudo
     CDS             complement(13465..14450)
                     /locus_tag="OO566_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14768..15283
                     /gene="zur"
                     /locus_tag="OO566_001675"
     CDS             14768..15283
                     /gene="zur"
                     /locus_tag="OO566_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6604993.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15325..15534)
                     /locus_tag="OO566_001676"
     CDS             complement(15325..15534)
                     /locus_tag="OO566_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6604994.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15650..16975)
                     /gene="dinF"
                     /locus_tag="OO566_001677"
     CDS             complement(15650..16975)
                     /gene="dinF"
                     /locus_tag="OO566_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6604995.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17048..17656)
                     /gene="lexA"
                     /locus_tag="OO566_001678"
     CDS             complement(17048..17656)
                     /gene="lexA"
                     /locus_tag="OO566_001678"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6604996.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17766..18134)
                     /gene="dgkA"
                     /locus_tag="OO566_001679"
     CDS             complement(17766..18134)
                     /gene="dgkA"
                     /locus_tag="OO566_001679"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6604997.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18305..20728
                     /gene="plsB"
                     /locus_tag="OO566_001680"
     CDS             18305..20728
                     /gene="plsB"
                     /locus_tag="OO566_001680"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6604998.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20883..21755)
                     /gene="ubiA"
                     /locus_tag="OO566_001681"
     CDS             complement(20883..21755)
                     /gene="ubiA"
                     /locus_tag="OO566_001681"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6604999.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21768..22265)
                     /gene="ubiC"
                     /locus_tag="OO566_001682"
     CDS             complement(21768..22265)
                     /gene="ubiC"
                     /locus_tag="OO566_001682"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6605000.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22488..24068)
                     /locus_tag="OO566_001683"
                     /pseudo
     CDS             complement(22488..24068)
                     /locus_tag="OO566_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24297..25217)
                     /gene="malM"
                     /locus_tag="OO566_001684"
     CDS             complement(24297..25217)
                     /gene="malM"
                     /locus_tag="OO566_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6605001.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLSVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25369..26709)
                     /gene="lamB"
                     /locus_tag="OO566_001685"
     CDS             complement(25369..26709)
                     /gene="lamB"
                     /locus_tag="OO566_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6605002.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26781..27896)
                     /gene="malK"
                     /locus_tag="OO566_001686"
     CDS             complement(26781..27896)
                     /gene="malK"
                     /locus_tag="OO566_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6605003.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28261..29451
                     /gene="malE"
                     /locus_tag="OO566_001687"
     CDS             28261..29451
                     /gene="malE"
                     /locus_tag="OO566_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6605004.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29605..31149
                     /gene="malF"
                     /locus_tag="OO566_001688"
     CDS             29605..31149
                     /gene="malF"
                     /locus_tag="OO566_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6605005.1"
                     /translation="MDVIKKKHWWQSDALKWSVPGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITTILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31164..32054
                     /gene="malG"
                     /locus_tag="OO566_001689"
     CDS             31164..32054
                     /gene="malG"
                     /locus_tag="OO566_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6605006.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8224 a         8290 c         7802 g         8002 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaacttatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttatacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacgttgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gacccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaggttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caagctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataattgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgaaataa agaatgggtc gaaactttac
    10501 catatgaagc agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa cctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aatagtgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccct
    12181 ggaacaaacc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggga agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacactaa
    13021 accgaccact ccagtgaacg tcagtttttt caggcattgc gctggtttgg ttgatttttt
    13081 gcatttcaga attaccgtgc attttcaaat gtagagatta ttttatcgat atatcatggg
    13141 gttatgttat tcagcttcac tgttcaggag gctcaatagc ggggtactat accataacaa
    13201 caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc tctcaaggca tgaagctctc
    13261 acgaagtgat ggaaataatc ttattagccg ttaactttgt taaggccaat gataaacaat
    13321 ccaggttcga cgataaataa aaaatcacac attaaactct ggtgatatat ctccctgcta
    13381 atagcattga tagagaaaaa agaacccaat aaatattggg tccttttata taatgccttc
    13441 catactattg aagaacttca catattattt ctccgattta accccgaaca agtcataaat
    13501 taatttagaa gtgtctgtaa gtattttaat ctcttccttt gaggttgggt caaacgactt
    13561 cgcgaagcta attaattgtg gtgcagcatc tcgcattttg cttaaaatat caggatcgag
    13621 cgttccttca ttcaccagat gtgacatctt atccagatag ccatcaaaat tcatttctcc
    13681 cccatctgcc agacgcttca tctctcccag gtacttcttc atatcacgtt gatataattt
    13741 ttcaaactga gctttcaggt agctctcatt attttcatta atatacactg tttctgataa
    13801 atctgcgtag gcactagaat atgaaagtga aattaacagc gaagcaaata attttaagct
    13861 gttgtcatca tgttcctggc aggcattaac aaacgttaaa aaaccagaac ctattgcttg
    13921 aaaatgtata ttcgctaaag gttcattatc cttagattcc tcataaaata gctgaaccgt
    13981 ggagggaagg ccattcttag tttctacatc aaaagtacat tgttcaatgg gcattgcatc
    14041 tttattatcc ccaggattac atatactatt aatcgcgaaa tgaaaaatag cccggtcaac
    14101 aactgatgca ataaagtcac gatcgtttaa atcctcatca gtgcaatcgt cagtatactt
    14161 attgacccac attttgagat catttatttg ctcaattaaa aacacatttt tttacgtgca
    14221 tgccataaat tatgcgtgat tcgttgaata aacaaatcta tttttgcaat aattatatcc
    14281 cgatgacgca gcgctgcata atcaatagat ttaccatata tattatttaa tttacctttg
    14341 attgaacctc tattatccaa tttatctttg gttgcatctt tattttcgaa ttgatttgaa
    14401 ttgagtgttt tattaaagta tccagttgca ccgggtatta tcttcaacat gattcatcta
    14461 ccttttttgt cgtaccgtgt cctccttata attcttttgc catctggtca ctatcaaaaa
    14521 tcgctcataa tttctgaact acaggtatgc gtttaaaccc agttaaataa atagcgagtt
    14581 aacttgaagc aatttattta aaaatgaatt attaattatt atcgtgttga taatggtacg
    14641 acaaggcaac acaacacctt acctacaaaa cagaaaatca acattacggc aacaataagg
    14701 gttctcggtg tttttcctgg aacatggtaa agtaaggaca ttcttaaccc ccactttgag
    14761 gtgcccgatg gaaaagacca caacgcagga gttattagcg caggctgaaa aaatctgcgc
    14821 gcagcgtaat gtgcgcctga ccccacagcg cctggaagtg ttgcgcctga tgagtctgca
    14881 ggatggcgct atcagcgctt atgatctgct tgatttactg cgcgaagctg aaccgcaagc
    14941 caagccgcca acggtttatc gcgcgctgga ttttctgctt gagcaaggtt ttgtgcataa
    15001 ggtggaatcc accaacagtt atgtgctctg tcatctgttc gaccagccca cgcatacgtc
    15061 agccatgttt atttgcgatc gctgcggcgc ggtgaaagaa gagtgtgcag aaggcgtgga
    15121 agacattatg catacgctgg cggcaaaaat ggggtttgcc ctgcggcata atgtgattga
    15181 agcacatggg ctctgtgcgg catgcgtgga agtggaagcg tgtcgtcatc ctgaacagtg
    15241 ccagcatgat cactctgtgc aggtgaaaaa gaaaccgcgt taagagggcg tacatccttg
    15301 tacacgtcgg gcaggaggga ttaattacca gcgatattca ttgcgggttt cccaatccac
    15361 gacctctttt tctgcctgat ctttctgata accataacgt tcctggattt taccgaccag
    15421 ttgatcacgt ttaccttcaa tgatcgtcat atcatcatcg gtcagtttgc cccattgctc
    15481 tttcacttta cctttaaact gtttccagtt accgccggct tcatctttat tcataatcaa
    15541 gacctcatcg ttaggttgtg aatgagagta cgttcacttt tcttctgaac gtgagattaa
    15601 gtgtagtcag caatttggct taggataatt tattcagaat ttttaaccgt cacgttgcgg
    15661 caaaccaggt accgttgcgc cagtgacgcc gccagatagc cgccagagaa agcccgcgca
    15721 acgccagaaa gacggttaat gccagccaca aaccatgatt acccagccac ggcagcgtaa
    15781 ggagcgtcag cgcaaaacct gcggcggcca ccgccatact gttacgcatt tcggcggcac
    15841 gcgttgcgcc tataaacatg ccgtccagca gataacacca gacgccaacc aacggcaaaa
    15901 tcacctgcca gataagatag cggtcagcca gctgctgaat ctgggttaac gacgtcagca
    15961 acgcaatgat gtgttcccct gccagcaaat aaaccaccga aaacagtaac gctacaatcc
    16021 ccgactggcg gcacgctgcc cgccatacat ccagcaactg gctaccgtcg cgcgcaccgt
    16081 atgcctgacc ggagtgcgct tcaaccgcgt aggcaaaacc atcaagcgca taggcggtaa
    16141 aggtgagtag cgtcatcaga accgcgttaa cagcgataat gtcactcccc agtcgcgcac
    16201 caagtacggt gatcgcgccg aaacagagtt gcaacaacag cgaacgcagc atgatatcgc
    16261 ggttaagcgc cagcaagcga cggaagtttc ctcgccaggc agttttcagc atttcgccgg
    16321 aaattccgcg tagtttgagg attttacgca ccattagcag accaatcagc aatgttgcat
    16381 attccgcaat aaccgtcgcc agcgccgcgc cctgcacgtt catatgcagc cccatcacca
    16441 gccagacatc cagcacaatg ttgaggatat tgccgaccac taacaaaatt actggcgcac
    16501 gggcatattg cacgccgagt aaccaaccga gtaataccag attcgccagc gacgccggag
    16561 cgcttaacca gcggatttca agaaagcgcc gcgcctgttc caggaccgct tcactaccgc
    16621 caacaatatg cagcgccaga tcgataatcg gcgtacgcaa cagcgcaatt aacgccccgg
    16681 cccccaacgc caacagcaac ggttgcacca gcgcacgggc taatgcctga ggatttttgg
    16741 caccataagc ctgcgcagtc agcccggtgg tgctcatgcg taaaaacagc aacagcataa
    16801 agagaaagct ggtcgccgtt gcaccaaccg ccacgccgcc caaataaacc ggactatcaa
    16861 gatgaccaat taccgccgta tcgaccagtc ccagcaacgg aacggtgata ttggagaaaa
    16921 tcatgggtaa ggcgagatgc cagagtgctt tatcagatga agtgaggaat gccatgcaga
    16981 caagcctgat gaagagagat gaaaaacaaa ccgcgacgcc aggcggcatc gcggtctcag
    17041 agatatgtta cagccagtcg ccgttgcgaa taaccccaac cgccagccct tcaatggtga
    17101 agctctgctg acgaaggtca acgacaattg gtttaaactc gctattttct ggcaacagtt
    17161 cgactttatt gccctgtttt ttcaggcgct taacggtaac ttcgtcatca atacgtgcga
    17221 caacgacctg accgttacgt acatcctgag ttttatgcac tgccagcaag tcgccatcca
    17281 taatgccgat atctttcatc gacatcccgc tgacgcgcag caggaaatca gcattcggct
    17341 tgaacaagga aggatcgacc tgataatgac cttcaatatg ctgttgcgcc agaagcggtt
    17401 caccggcagc cacacgacct accagcggca acccttcttc ctcttcctgc aacagacgaa
    17461 tcccgcgtga tgcgccggaa acaatttcaa taacgccttt gcgtgccagc gccttcagat
    17521 gttcttcagc cgcgtttggg gaacggaacc ccaaacgctg cgcgatttcc gcacgcgtcg
    17581 gcggcatacc tgtctggctg atgtgatcac ggatgagatc aaacacctct tgttgcctgg
    17641 ccgttaacgc tttcattccg ccccctgggt gtatatacag ttatactgtg agtatataca
    17701 gcaaaaggcg attttggaac cataaactgc acaataaacc agagatttat cgaaatctgg
    17761 aagggttatc caaaatgcga ccataacagg atgcaccagg taatcacggc gacgataatg
    17821 gcaatcagca ccgcggcgga tcccatatct tttgcgcgtc cggaaagctc atggtattca
    17881 gagccaattc ggtcaaccac tgcttcgatg gcgctattga ggatttccac aatcatcacc
    17941 agcatcacgg agctgataag cagcacgcgg gtaattgcgt ccacatccag ccagcaggcg
    18001 atgaccaccg ccaacaatac cgctacgcct tcctgacgga atgccgcttc gttgatccat
    18061 gcagcgcgta aacctttcca ggaatagcca gcagctttga taattcgggt gaatccagtg
    18121 gtattattgg ccattgaaag aaccttttta cattatgagc gtcaatatca gtgtaccgtt
    18181 aaatccgatg attcgggttt gcgtaacgcc gcgcgaaaca tgagcggata ccacagaatt
    18241 tcccatgact ttctgctatc cttgccgcgc atttgcatta ttaaccagag gctttacatc
    18301 gtttatgtcc ggctggccac gaatttacta caaattactg aatttaccat taagcatcct
    18361 ggtaaaaagc aagtctattc cggcagatcc tgccccggaa ctggggctgg atacctctcg
    18421 tccaattatg tacgttttac cgtacaactc gaaagcagat ttgctgacgt tgcgcgccca
    18481 gtgtctggca catgatttgc ctgacccgtt agagccgctg gaaatcgacg gcacgctact
    18541 gccgcgctat gtgttcattc acggcgggcc gcgtgtattc acctattaca cgccgaaaga
    18601 agagtctatt aagcttttcc acgactatct cgatttgcac cgtagcaacc caaatctgga
    18661 tgtgcagatg gtgccagtat cggtgatgtt tggtcgcgcg ccggggcgtg aaaaaggcga
    18721 agtgaacccg ccgctgcgta tgcttaacgg cgtacagaaa tttttcgctg tactgtggct
    18781 cggtcgcgac agttttgtgc gtttctcgcc gtcagtgtcg ctgcgccgta tggcggatga
    18841 acacggcacg gataaaacta tcgctcagaa actggcgcgc gtggcgcgta tgcactttgc
    18901 ccgtcaacgt ctggctgccg taggcccacg tctgcctgct cgtcaggatc tgtttaataa
    18961 gctgctcgcc tcccgcgcca ttgccaaagc ggtagaagat gaagcgcgca gcaaaaaaat
    19021 ctcccatgaa aaagcgcagc agaacgcgat tgcgctgatg gaagagattg cggcgaattt
    19081 ctcttacgag atgatccgcc tgactgaccg cattctgggc ttcacctgga accgatttta
    19141 tcagggcatc aacgtccata acgccgagcg cgttcgccag ctggcccacg acggtcatga
    19201 gctggtgtat gtgccttgcc accgcagtca catggactac ctgctacttt cttacgtgct
    19261 gtaccaccag gggctggtgc cgccgcatat cgccgccggg atcaacctga acttctggcc
    19321 ggccgggccg attttccgcc gtctgggggc gttctttatt cgccgtactt ttaaaggcaa
    19381 taaactttat tccaccgttt tccgtgagta tctcggcgaa ctgttcagcc gtggttattc
    19441 cgtcgagtac ttcgtggaag gcggtcgttc ccgcacgggg cgtttgctgg atccgaaaac
    19501 cggtacgctg tcgatgacca ttcaggcgat gctgcgtggc ggcacgcgtc cgattacgct
    19561 gattccgatc tatatcggtt atgagcacgt catggaagtg ggtacttacg ccaaagaact
    19621 gcgcggcgcg acgaaagaga aagagagcct gccgcagatg ctgcgcggtt taagcaagct
    19681 gcgtaatctc ggtcagggtt acgtcaactt cggtgaacca atgccgttga tgacctacct
    19741 taaccagcac gtaccagact ggcgtgaatc tatcgatccc atcgaagcgg tgcgtccggc
    19801 ctggttaacg ccgacggtca ataatattgc tgccgatctg atggtacgca ttaacaacgc
    19861 aggcgcggca aacgccatga acctgtgctg tactgcgcta ctggcatcac gtcagcgctc
    19921 actcacccgc gagcagttaa ccgagcaact caactgctac ctggatctga tgcgcaacgt
    19981 accttactcc acggactcta ccgttccttc agccagcgcc agcgagctta tcgatcacgc
    20041 gctgcaaatg aacaagtttg aagtcgagaa agacactatc ggcgacatca tcattctgcc
    20101 acgcgagcaa gcggtgctga tgacctacta tcgcaacaac attgcgcata tgttggtgct
    20161 gccttcgctg atggcggcaa ttgtcaccca gcatcgccac atctcccgcg acgtattgat
    20221 ggagcacgtc aatgtgcttt acccaatgct gaaagcggag ctgttcctgc gctgggatcg
    20281 cgacgagttg ccggacgtta ttgatgcgct ggcaaatgag atgcaacgtc aggggctgat
    20341 taccctgcaa gatgatgagt tgcatatcaa cccggcgcat tctcgcaccc tacagctgct
    20401 ggccgcaggc gcgcgcgaaa cgctgcaacg ttatgccatc accttctggt tgttgagtgc
    20461 caacccgtcg atcaaccgcg gtacgctgga gaaagagagc cgcaccgttg cgcaacgtct
    20521 ctccgtgctg cacggcatca acgcgccgga gttcttcgac aaggcggtgt tcagttctct
    20581 ggtgctgacg ctgcgtgatg aagggtatat cagcgatagc ggcgatgccg aaccagcaga
    20641 aacgatgaag gtttatcagt tgctggcgga gttgattaca tcagacgtgc gtttgacgat
    20701 tgagagtgcg acgcagggcg aagggtaatc agagagaatt gccggatgcg gcgaaaacgc
    20761 cttatccggc ctaccatgac ctgcaaattc aataaattgc gattcaccgg gtaggcctga
    20821 gaagcgcagc gcatcaggca acccagaaga aagccggatg atcatccggc ttttttacat
    20881 catcagaaat gccagtaact cattgccagt cctaaaaaca gtaccagacc aacatagtta
    20941 ttattcataa atgctttaaa gcaggcttca cgctcgcggt tggcaatcag tttttgttga
    21001 taaacaaaca gcgcgccagc caccacaatt gaccagtaat atccccagcc taagccattt
    21061 aactcaccga tgatcgccat cagtgccagt acgccaatct gcaaaatacc aataatcaat
    21121 ttatcgtatt ggccgaacag gattgccgtg gatttaatgc caatcttcac atcatcatcg
    21181 cggtcaacca tcgcatactg cgtgtcgtaa gccaccgccc agagaatatt ggcgaggaac
    21241 attaaccagc aactcaatgg caccgactca ctcacagcgg caaaagccat tggaatcgac
    21301 cagccaaacg ccgcgcccag caccacttgc gggagatggg tataccgctt cataaacggg
    21361 tacacccacg ccaatgccag agcggcaatc gacaacagaa tggtcatcgt attcagcgtc
    21421 agaaccagta aaaacgaaat cagtaccagc actacaaaca gcgcgcgcgc ctctttctct
    21481 gttaccgcgc cgctgggaag tggtcggttc gccgtgcgct taacatgacc atcaaacttg
    21541 cgatcagcat aatcattcac cacgcaacca gcagcgcgca tcagccagac acccgcgaca
    21601 aacaccgcca ggatccagag ctgaggaacg cccggtgtcg ccacccacaa cgcccataat
    21661 gttggccaga gcagcaataa cgcgccaatt ggcttatccg tacgcattaa gcgatgaaac
    21721 gccagcagct tattctgcgt cagactccac tccatatttt tttcctctta gtacaacggt
    21781 gacgccggta aaaacagttc tgttagcaac agcggtttac cgcttaatcg caggcgggaa
    21841 cgtcgccccc acagtccggc atcacggcct atctcgataa aatcccgggt tagtgtcgat
    21901 gatgtgaaca gatagcgtcc taacggcgtt ttacccaatt tttgtaacgc tagttccggc
    21961 ccgcttaatg ttgatacagg aacgacggta cgaccggcaa gccacggttc accatcggca
    22021 cataacaaaa tttcacgtaa ccagtaacga gactctttcg gcagtagcgg cagttcttcg
    22081 gggatttcat tctgctcgac aaacccctcg cggatcatcg tcacactcac cgtttttccc
    22141 tgctgttcaa aacgttttgt catggaatcc tccagcaaca gccagtcgaa cagttgcgga
    22201 tccagggcag ggatctcttt aaaatagcgc agcgcacgca gttgcgttaa cgcggggtgt
    22261 gacatgccga actctccgtt acataacgta aaggcattgt atcgcagaaa agtgtttacg
    22321 ggagatgtaa agcaaattaa gtgatctaat gcgcaacaaa aacgcaacaa tcgcatgaag
    22381 tggtaaaacg ctgttgaacc ttacgctttg ccgatgcccg tgctggcgag ggcaaccata
    22441 ggggcatcac cgcccatgat gctaggaacg gtgatgtttt cctttactta aataccttta
    22501 ttctttatac gagtagctcg ccaactcgca gtaaaaattg ggaaaagcgc atatttacca
    22561 tgagatttgg agctgaaaag tgtttcctga gtaagattaa taggcaacat cttatgtctg
    22621 gatttataga tacacgtgga attccaattg gtatcttttg taggattcaa catatcatgt
    22681 aagcgctggg agcttaccac cataacaggc ccatcatcga cagaggagaa caggataaat
    22741 atattatcgt ttcgtgttaa tttattagtg ttaaaggttc ttaacttttc ttcagtttct
    22801 ataaatatag gtgaaacatc atcactacga atccactctt tataaagata ccaggcattt
    22861 gctttgatca tatcatccgt tgatcgtaag gcctgatcga taagttggat aaaaaaattg
    22921 ttatttttat caaccagccc tttattcata ttaaatctgt taataaaatg gatcatctgt
    22981 tgggtatcga aattcacctt actgaatcga tgttgacgca taaaattatc gaacttgtca
    23041 tcttccccag aatgtatata actacgtata attggatctt ttatataagg acaatggcta
    23101 aatgttttct tcatattctc aataaagttt ttattaacaa tattgagaat ttttaattgc
    23161 atcaccagtt ttactgccag aacggatttc aatatcattg cattatctgg caatgtatca
    23221 atcattttta taatgttatt gatataacca tcgagatctt cattcccaaa tatatctgaa
    23281 taatcaatag ttaattccaa ttgttctgta atgcaatctt aaaaattccc tgtcgacatt
    23341 cgcacatgat caaacataac agctgtcaaa ttcgatttaa taaatgatat ttctttggca
    23401 gacagaaact ctgctctggt gaagtgcgaa gcgtaaagat aacaattatc gaagtttgaa
    23461 tttctaatct gacattcgcg aaaattacta tgaccaagtt gactctgcga gaagtcaact
    23521 gtatcaagaa tattttggct aaaatcaaca cattcgccac tcgttttgag aaaaatcaca
    23581 ttgtgtaaac gtcgacattt aaatatgaca ttatgaactg ttgccttagt aaaattgcaa
    23641 ttctcaattt ctttacattc gaaagttaca tgttgtagat ttgatgataa aaaagacacc
    23701 ttagaaataa cgtcaccttt aaaatgacaa tttgttaaat caaaatcaga caggtcgata
    23761 tacccctgcg catctttatc catcagaaaa ttacaagcgc attctatttt ttcatttctg
    23821 tagaatttta cacttatagt atcattacta attgagactt cacgtaacat atttattgca
    23881 tgattttttt cgttccgttg ttttatattt aaaaatatat cactgatata aataaagtta
    23941 tctctgttta aacctgtcac cccattgaaa ttacgtagta aatcaagatc tactatatta
    24001 tttccttgca ccacatcatg tttcatcaaa gagttattgc cgccagaatt tgtgatatga
    24061 cctatcataa aacctctgta atacagaaat aaaataacct ggaaaagtag cctgagcgtc
    24121 cagacactac cggcgaaagg aaaagaatct ctgataatgc attgaaataa tggacattct
    24181 tagtgaagaa gaagtgaagt actataaaaa atcgctcata tcccaggaag gaagcataaa
    24241 aaaaggtgcg ccaggagacg caccagttgt tgcaaaatca gcactgtggg gcgtaattac
    24301 cccttgcctt ttacactgct gataaaggtg gaacgggcag atgtcgatcc caggcgttca
    24361 gcttcatcaa gcagttttaa cgccttatca acatcacctt tcgcgacagc gtttttgatc
    24421 gcggtattaa aataactttc cgtgtcgttg agcatcggct cgcttttctt caccggcacc
    24481 ggagcaggtg cagccacagc tggtgccgcc gtgttgccta ccgtaaccgg agctggagcg
    24541 gaagaaccaa atagtggtcc taccaacacg ctggagctgg agttcgtttt gactttcagt
    24601 ttcagtaagc catcgctggt atgacgagca accggatcgg gaatatccgg gatcgagtta
    24661 ccgacgcctt tggcataggc tttagccggg tcgagcagtt gggtcgtctg ctggagatct
    24721 ttttccatgg taaagaccag aacataaagt ttttgctgcc ccaacgccgg tgtcaggcgc
    24781 ataacgcctt ccagccgatc tgcactcatc acgcctagtt cctggtaggt gaaataactg
    24841 ctggggaaga aggctgatgg ggtcatgttc tgatcaataa tcagcacgtt cggcgcaaaa
    24901 acgctggttt gtttgttcac ttcgctggtc agcgtcaggg tcagttcgcc aatgtttgcc
    24961 gggacgctgt acgcagcaac cggaccactg atgccgggaa cgctcagttg ttggccgccg
    25021 gtcgccagtt gggtggtctg ggttttagat tgatcgaccg gtgtccaggt gagttgttgc
    25081 agcgcggcag atggaatggc tggcgcgtcg ctggtgtttt gcggtacgta gttaacatcg
    25141 gcaaggctaa ttccaggcgc gcttgccagt aaccctgctg ataaacagag ggcgatgaga
    25201 cttttattca ttttcattgt tttcacctca aaatctggag ctcagcggta gccaggcaat
    25261 agcgcgctaa acccgataat cagaggggct ttcgcccctt tcagataatg acaacctgtt
    25321 tttatgccgg atgcggcgta aacgccttat ccggcccagg ttttgctatt accaccagat
    25381 ttccatctgg gcaccgaagg tccactcgtc gctgtcgcca cgaccgaagc tgccgccgtt
    25441 gaaatcagca ggaacggctt tgccgtagtt cgggttaacc ttgctatcgc cgttgtagtc
    25501 gtaaccccat ttctcatccc acttggcgta ggttgcgaag acacgaatag ccgggcgtga
    25561 ccagatgctg tcgccagcct gccattgttg tgcgagggta attttgtact gattgttctt
    25621 gtcgccggtg cgctgggatt cgacgttgtc gtagccgatt tccatcacgg tgctcatgat
    25681 tggcgtccac ttgtacatcg ggcgaatacc aacggtccac cacttggtgc cgttgtcgtt
    25741 atcccagttg atatcctggt acatacccac gtacatcatg tcccagttgt cgcccatgga
    25801 gatcgcaccg tggtcgagga tacgcagcat gtgaccgttg ttgttgatat tgtaggcaaa
    25861 tttttcgtta tcaaacgcga cgccagaacc ctgcgacaga cctttaccct gcgaggtcat
    25921 cgagtcagta gcgtactgaa caacaaactt gttaaagccc ttcaggacac tctgagtatg
    25981 ttcagcagtg aataaccagc cgtctttcga tgcgccatca accagacgat agttatcacg
    26041 caggttggca cgaccgtagt cgacacccag ttctaatgtg ccgcccgggt tgatttccat
    26101 ctgcgctaaa cgcacatcga aaacgtcgtt cgcggtttcg ttggtatagt cataaatatt
    26161 gttgctggca aaagaggaag aaccaccagc ttcagaggag cgggttgctg ccagagagag
    26221 tttaccgaag ccaacatcga tgttttccag accggcacca ggaccagaaa tatcccagta
    26281 gtagaagtcg atcatatgaa cgtcatgacg ttggtagaag cgcttacctg cccagatggt
    26341 ggaacctggc agccattcga tcaggttttt tccctgcacg tttgcttcac ggaaggccgg
    26401 atcggtagct tcccagtcat tctgttgcgc gacggaatag gccacgttag tgtcgaaata
    26461 gaagctctta tcgccctctt tccacacttc ctgacccaat tttaattcag cataagtttc
    26521 acattcgttg ccaagacggt atttactttg agcaccggta gtctggaaac actgttgttc
    26581 accgccgcta cctgtccagc caataccgga acgtgcatag ccgtggaaat caacagccat
    26641 tgcctgagca gacattacgc ccgctgcgac ggcaaccgcc agaggaagtt tgcgcagagt
    26701 aatcatcatt ctatctcctg agtcattgct tttctttttt cacatcacct gtgacaggct
    26761 ttgtgtgttt tgtggggtgc ttaaacaccc ggctccttat gcagtcgacg acatgcagtg
    26821 ccatcctcac ggaacagatg gcaacgctct ggcggcaggc cgatagcgaa tgtggcacct
    26881 tcttctacca acaccacgtc gttctggcgg tacaccaggt tttgacgaat ggaagggatc
    26941 tggatataga tttgggtttc gttgccgagt tgctcgacga cctgaacttc accctcaagg
    27001 atgacgtcag cgatatcact cggcagtaga tgttccgggc gaatacccag cgacatattg
    27061 gctccaacct ggacatcacg gctttcaaca ggcagccaga cttgttgacg atttggcatc
    27121 ggcagctcca cctgcacttg atcgattgcg gtggcggtca cttttaccgg caggaagttc
    27181 atctttggcg aaccgataaa tccggcgaca aaacggtctg ccggatagtg gtacagttcc
    27241 agcggtttcc caacctgtgc cacgcgaccg gcgtccagca ccacgatttt gtcggccagc
    27301 gtcatcgctt cgacctgatc gtgggtgacg taaatcattg tgcggcccag gcgtttatgc
    27361 agacgggaga tttcgatacg catttgcaca cgcagtgcag catcgaggtt ggagagcggt
    27421 tcatcgagca aaaatacgct tggctcggcc accagcgtac ggccaatcgc cacgcgctga
    27481 cgctgaccac cggagagcgc tttcggtttg cgatccagca aatgcgccag ttgcagcact
    27541 tccgccacct ggttgacgcg ttggttaatc acctcttttt ttgcgccagc cagtttcagg
    27601 ccaaatgaca tgttttctgc tactgacagg tggggataga gcgcgtaaga ctgaaacacc
    27661 ataccaacgc cgcgttctgc tggcggagtg tcattcatcc gtttctcacc gatgaacagg
    27721 tcgccgctgg tgatcgtctc aagcccggca atcatgcgca gtaaagtcga tttaccgcag
    27781 ccagacggtc cgacaaacac cacgaattca ccttcatgga tatcgagatt gatatctttc
    27841 gataccacga cctcgcccca ggctttcgtt acattttgca gctgtacgct cgccatgccc
    27901 ttctcccttt gtaacaacct gtcatcgaca gcaacattca tgatgggctg actatgcgtc
    27961 atcaggagat ggcttaaatc ctccaccccc tggctttttt atgggggagg aggcgggagg
    28021 atgagaacgc ggcttctgtg aactaaaccg aggtcatgta aggaatttcg tgatgttgct
    28081 tgcaaaaatc gtggcgattt tatgtgcgca tccccacatt accgccaatt ctgtaacaga
    28141 gatcacacaa agcaacggtg gggcgtaggg gcaaggagga tggaaagagg ttgccgtata
    28201 aagaaactag agtccgttta ggtgttttca cgagcacttc accaacaagg accatagatt
    28261 atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt
    28321 tccgcctcgg ctctcgccaa aatcgaagaa ggtaaactgg taatctggat taacggcgat
    28381 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
    28441 gtcaccgttg agcatccgga taaactggaa gagaaattcc ctcaggttgc ggcaactggc
    28501 gatggccctg acattatctt ctgggcgcac gaccgctttg gtggctacgc tcaatctggc
    28561 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacatgg
    28621 gatgccgtac gttacaacgg caagctgatt gcttacccaa tcgctgttga agcgttatcg
    28681 ctgatttata acaaagacct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
    28741 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
    28801 tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc
    28861 aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc
    28921 ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa
    28981 gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac
    29041 atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca
    29101 tctaaaccgt tcgttggcgt gctgagcacc ggtattaacg ccgccagtcc gaacaaagag
    29161 ctggcgaaag agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat
    29221 aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat
    29281 ccacgtattg ccgccaccat ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg
    29341 cagatgtccg ctttctggta tgccgtacgt actgcggtga tcaacgccgc cagcggtcgt
    29401 cagactgtcg atgaagccct gaaagacgcg cagactcgta tcaccaagta atgctgtgaa
    29461 atgccggatg cggcgtgaac gccttgtccg gcctacaaaa ctgaaacgca tgtaggcctg
    29521 ataagacgca tcagtgtcgc atcaggcagt tgttgtcgga taaggcgtga aagccttatc
    29581 cgtcctggaa tgaggaagaa ccccatggat gtcattaaaa agaaacattg gtggcaaagc
    29641 gacgcgctga aatggtcagt gccaggtctg ctcggcctgc tggtgggtta ccttgttgtt
    29701 ttaatgtacg cacaagggga atacctgttc gccattacca cgctgatatt gagttcagcg
    29761 gggctgtata ttttcgccaa tcgtaaagcc tacgcctggc gctatgttta cccgggaatg
    29821 gctggaatgg gattattcgt cctcttccct ctggtctgca ccatcgccat tgccttcacc
    29881 aactacagca gcactaacca gctgactttt gaacgtgcgc aggaagtgtt gttagatcgc
    29941 tcctggcaag caggcaaaac ctataacttt ggtctttacc cggcgggcga tgagtggcaa
    30001 ctggcgctca gcgacggcga aaccggcaaa aattacctct ccgacgcgtt taaatttggc
    30061 ggcgagcaaa aactgcaact gaaagaaacg accgcccagc ccgaaggcga acgcgcgaat
    30121 ctgcgcgtga ttacccagaa tcgtcaggcg ctgagtgaca ttaccaccat tctgccggat
    30181 ggcaacaaag tgatgatgag ctccctgcgc cagttttctg gcacgcagcc gctctacaca
    30241 ctcgacggtg acggcacgtt gacgaataat cagagtggcg tgaaatatcg tccgaataac
    30301 caaattggct tttaccagtc cattaccgcc gacggcaact ggggggatga aaagctaagc
    30361 cccggttaca ccgtgaccac cggctggaaa aactttaccc gcgtctttac cgacgaaggc
    30421 attcagaaac cgttcctcgc cattttcgtc tggaccgtgg tgttctcgct gatcactgtc
    30481 tttttaacgg tggcggtcgg catggttctg gcatgtctgg tgcagtggga agcgttgcgc
    30541 ggcaaagcgg tctatcgcgt cctgctgatt ctgccctacg cggtgccatc gttcatttca
    30601 atcttgattt tcaaaggatt gtttaaccag agcttcggtg aaatcaacat gatgttgagc
    30661 gcgctgtttg gcgtgaagcc cgcctggttc agcgatccga ccaccgctcg cacgatgctg
    30721 attatcgtca atacctggct gggttatccg tacatgatga tcctctgcat gggcttgctg
    30781 aaagcgattc cggacgattt gtatgaagcc tcagcaatgg atggcgcagg tccgttccag
    30841 aacttcttta agattacgct gccgctgctg attaaaccgc tgacgccgct gatgatcgcc
    30901 agcttcgcct ttaactttaa caacttcgtg ctgattcagc tgttaaccaa cggcggcccg
    30961 gatcgtcttg gcacgaccac gccagccggt tataccgacc tgcttgttaa ctacacctac
    31021 cgtatcgctt ttgaaggcgg cgggggtcag gacttcggtc tggcggcagc aattgccacg
    31081 ctgatcttcc tgctggtggg tgcgctggcg atagtgaacc tgaaagccac gcgaatgaag
    31141 tttgattaag ggagataaca aaaatggcaa tggtccaacc gaaatcgcaa aaagcacgtt
    31201 tgtttattac tcacctgcta ctgctacttt ttatcgcggc gattatgttc ccgctgctga
    31261 tggtcgtcgc tatctcgctg cgtcagggaa acttcgcgac cggtagcctg atcccggagc
    31321 aaatctcctg ggatcactgg aaactggcgt taggttttag cgttgaacag gctgatggtc
    31381 gcattacgcc accgccattc ccggtactgc tgtggctgtg gaactcggta aaggtcgctg
    31441 ggatttccgc gattggcatt gtggcgctct ccaccacctg cgcctacgct ttcgcccgta
    31501 tgcgctttcc aggcaaagcg acgctgctga aaggaatgct gattttccag atgttcccgg
    31561 cagtactttc actggtcgcg ttgtatgcgt tgtttgatcg tctgggtgag tacattccat
    31621 tcattggcct gaatactcac ggcggcgtaa ttttcgcgta tctgggtggg attgcgctgc
    31681 atgtctggac catcaaaggc tatttcgaaa ccatcgacag ttcgctggaa gaagcggctg
    31741 cgctggatgg tgcgacaccc tggcaggcct tccgccttgt cctgttgccg ctgtcagtac
    31801 cgattctggc ggtggtattc atcctgtcgt ttatcgctgc cattactgaa gttccggtcg
    31861 cgtcgctgtt actgcgtgac gtaaacagct acaccctggc cgtggggatg cagcaatacc
    31921 tcaacccgca aaactacctg tggggtgact ttgccgccgc tgccgtgatg tctgcattac
    31981 cgatcaccat cgtcttcttg ctggctcaac gctggctggt caacggcctg acggcaggtg
    32041 gtgtgaaagg ttaaagatgt tgttctgcca atgttatgcc gctgcaccct caacttacgt
    32101 tatcccaact tgtgactatt attcggcgct ccacggagcg ccttttttct ttcgtctgca
    32161 atctgaatcg ttcgccggtt aatattccca tcatagaact tattattttt acgttatttg
    32221 ttttcccact tacgataatt ctctttcgtg ctctgagtca cggcaatagt attgttttca
    32281 tcaatttttg ataattatca caattaagat cacagaaa
//
LOCUS       DAJTXY010000034        32162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTXY010000034 DAJTXY010000000
VERSION     DAJTXY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.034"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..548
                     /locus_tag="OO566_001690"
     CDS             <1..548
                     /locus_tag="OO566_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6605007.1"
                     /translation="TLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGSNYAQDLT
                     MLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGERIDYRVKM
                     YNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVDKFSVMDK
                     QAGYYMGKLSVIFTPSLPTIN"
     gene            complement(636..1934)
                     /gene="exuT"
                     /locus_tag="OO566_001691"
     CDS             complement(636..1934)
                     /gene="exuT"
                     /locus_tag="OO566_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6605008.1"
                     /translation="MSKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            2417..3829
                     /gene="uxaC"
                     /locus_tag="OO566_001692"
     CDS             2417..3829
                     /gene="uxaC"
                     /locus_tag="OO566_001692"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6605009.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHFELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3844..5331
                     /gene="uxaA"
                     /locus_tag="OO566_001693"
     CDS             3844..5331
                     /gene="uxaA"
                     /locus_tag="OO566_001693"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6605010.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            5414..5965
                     /locus_tag="OO566_001694"
     CDS             5414..5965
                     /locus_tag="OO566_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6605011.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(5970..7214)
                     /gene="sstT"
                     /locus_tag="OO566_001695"
     CDS             complement(5970..7214)
                     /gene="sstT"
                     /locus_tag="OO566_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6605012.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(7810..8775)
                     /gene="alx"
                     /locus_tag="OO566_001696"
     CDS             complement(7810..8775)
                     /gene="alx"
                     /locus_tag="OO566_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6605013.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(9046..9246)
                     /locus_tag="OO566_001697"
     CDS             complement(9046..9246)
                     /locus_tag="OO566_001697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6605014.1"
                     /translation="MQDLTQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLL
                     TEIRRQTGVIFPADSVKLYLPK"
     gene            complement(9277..10044)
                     /locus_tag="OO566_001698"
     CDS             complement(9277..10044)
                     /locus_tag="OO566_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6605015.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSEC"
     gene            complement(10123..10815)
                     /locus_tag="OO566_001699"
     CDS             complement(10123..10815)
                     /locus_tag="OO566_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6605016.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(10892..11395)
                     /locus_tag="OO566_001700"
     CDS             complement(10892..11395)
                     /locus_tag="OO566_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6605017.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            11480..12616
                     /gene="rlmG"
                     /locus_tag="OO566_001701"
     CDS             11480..12616
                     /gene="rlmG"
                     /locus_tag="OO566_001701"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6605018.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            12901..13215
                     /gene="higB"
                     /locus_tag="OO566_001702"
     CDS             12901..13215
                     /gene="higB"
                     /locus_tag="OO566_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6605019.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            13212..13628
                     /gene="higA"
                     /locus_tag="OO566_001703"
     CDS             13212..13628
                     /gene="higA"
                     /locus_tag="OO566_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6605020.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13673..15691)
                     /gene="fadH"
                     /locus_tag="OO566_001704"
     CDS             complement(13673..15691)
                     /gene="fadH"
                     /locus_tag="OO566_001704"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6605021.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(15911..18262)
                     /gene="ygjK"
                     /locus_tag="OO566_001705"
                     /pseudo
     CDS             complement(15911..18262)
                     /gene="ygjK"
                     /locus_tag="OO566_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(18279..18698)
                     /locus_tag="OO566_001706"
     CDS             complement(18279..18698)
                     /locus_tag="OO566_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605022.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(18676..19029)
                     /locus_tag="OO566_001707"
     CDS             complement(18676..19029)
                     /locus_tag="OO566_001707"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605023.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(19030..19350)
                     /locus_tag="OO566_001708"
     CDS             complement(19030..19350)
                     /locus_tag="OO566_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605024.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(19484..20917)
                     /locus_tag="OO566_001709"
     CDS             complement(19484..20917)
                     /locus_tag="OO566_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6605025.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(20980..21429)
                     /locus_tag="OO566_001710"
     CDS             complement(20980..21429)
                     /locus_tag="OO566_001710"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6605026.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATHALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(21426..24512)
                     /gene="ebgA"
                     /locus_tag="OO566_001711"
     CDS             complement(21426..24512)
                     /gene="ebgA"
                     /locus_tag="OO566_001711"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6605027.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(24695..25678)
                     /gene="ebgR"
                     /locus_tag="OO566_001712"
     CDS             complement(24695..25678)
                     /gene="ebgR"
                     /locus_tag="OO566_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6605028.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            25897..26228
                     /locus_tag="OO566_001713"
                     /pseudo
     CDS             25897..26228
                     /locus_tag="OO566_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(26270..27649)
                     /gene="ygjG"
                     /locus_tag="OO566_001714"
     CDS             complement(26270..27649)
                     /gene="ygjG"
                     /locus_tag="OO566_001714"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6605029.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            28066..29586
                     /gene="aer"
                     /locus_tag="OO566_001715"
     CDS             28066..29586
                     /gene="aer"
                     /locus_tag="OO566_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR6605030.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(29740..30363)
                     /gene="nfeR"
                     /locus_tag="OO566_001716"
                     /gene_synonym="yqjI"
     CDS             complement(29740..30363)
                     /gene="nfeR"
                     /locus_tag="OO566_001716"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6605031.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            30640..31386
                     /gene="nfeF"
                     /locus_tag="OO566_001717"
                     /pseudo
     CDS             30640..31386
                     /gene="nfeF"
                     /locus_tag="OO566_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(31440..31515)
                     /locus_tag="OO566_001718"
     tRNA            complement(31440..31515)
                     /locus_tag="OO566_001718"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31480..31482),aa:Ile,seq:cat)
     gene            31640..32146
                     /gene="mug"
                     /locus_tag="OO566_001719"
     CDS             31640..32146
                     /gene="mug"
                     /locus_tag="OO566_001719"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6605032.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         7771 a         8400 c         8467 g         7524 t
ORIGIN      
        1 ttaccctgaa tacgctcgac cccaaccata ttgacgtgtt cttcccggag ttcgcccacg
       61 ccacgccaag ggtgcagtta gacttgcatc caacaggaag cgttaacggc agcaactacg
      121 cgcaagatct gaccatgttg gacatgtgcc tgtacgatgg ttttaacggt aatgccatca
      181 gttatgaaat catgctcaaa gatgaagggc gaccagctgc agggcgcaga gacggttact
      241 tctctatcta tcgtcaggga gggaccacca ccgacgaggg agaacgcatt gattaccggg
      301 tcaaaatgta caacccggaa accggtgggc aaattgatgt gcgcaataat gaaaatatgg
      361 tctggaacag cattaacctg aaacgtgtgc gtccggtggt actgcccggt attcgctatg
      421 ccgtgatgtg tgtgccgaca cccttgactc tggcagtcga taaattcagc gtgatggata
      481 aacaggccgg gtactacatg ggcaaattgt cagtaatctt tacgccttcc ttgccaacca
      541 tcaattaacc ctgcaaaccg gacaaacgct tttttctata taaacgacga agccgcctga
      601 taaggcggct tcgtcgttat aacttacgtt aaacgttaat gttgcggtgc gggatcgtta
      661 tgtgtttcct gtgccacctc aatcgccggt ttgttctgca atacggtcca gataaccagc
      721 gcacccagca ggtcaaatac tgccagaact gcgaacagcg ggctgaagcc gatggtgtca
      781 gccagtgcac cgacaaccag cgcaaacagc gtacttgcca gccatgcgga catcccggtt
      841 aaaccgttcg ccgtcgccac ttcgttacga ccgaacacgt cagaagagag cgtaatcagc
      901 gcgccagaca gtgcctggtg ggcaaaacca ccgatacaca gcagcataat tgcgacatac
      961 gggttggtga acagaccgat catacccggg ccaatcatca gcacagcacc cagcgttacg
     1021 accatcttac gggaaacgat caggttcaca ccaaaccaac gctggaacag cggcggcagg
     1081 taaccaccga ggatacaacc gaggtcagca aacagcatcg gcatccaggc gaacatcgcg
     1141 atctctttca ggttaaagcc gtaaacttta aacatgaaca gcgggatcca cgcgttaaaa
     1201 gtaccccagg ccggttctgc taggaaacgc ggcagtgcga taccccagaa ctgacggtta
     1261 cgcaggatct gaccaacgga cattttcttc gccgtgctca cctggtgctg ggcttcctga
     1321 ccattaataa tatagtcgcg ttcttcatcg gtcagatgct tctggtcgcg cggatgttta
     1381 tagaaaatca gccatgccat cgcccagata aagctcaatg caccggagat gataaatgcc
     1441 atctgccagc tgtgcatcac gattgcccat accaccagcg gcggcgcaat catcgcacca
     1501 atcgaagaac ctacgttaaa gtagcctact gcgatggaac gctctttcgc cgggaaccat
     1561 tcggagctgg ctttcagacc cgccgggatc atcgctgctt ccgcggcacc gaccgcacca
     1621 cgagcaacag ccaggccacc ccagctacct gccagcgcgg ttgcaccaca gaacacggcc
     1681 cacagcacag caaacattgc ataaccgatt ttcgtaccca gcacatccag tacataacct
     1741 gctaccggtt gcatgaccgt ataagcagca gaataggctg cgatgatata ggaatactgt
     1801 tgggtggaga tgtttaactc ttccatcaga gttggcgcag ctgccgccac agtgttacgc
     1861 gtcaggtaac caagcacggt gccgagcgtc accagtgcga tcatatacca acgtaaccct
     1921 ttaattttac tcatgtaaaa cctcatcgtt ttgttatatc cgcaggaagc gcggccatca
     1981 tcccgccaga ggctgaagat gttagttacg accgggtaac cgaaaaaaaa ccggcacgcc
     2041 ccgaacgttg ccatgaaagt ttcgtgctaa ttcggcttcc gtaccggtta acccgattca
     2101 tctaacaaat gaatgaatcg gcaatttgca ttccgtcgac ttatgatttg cgacggcaga
     2161 aagataactt gtcatacaac cttaaaaggt gagagccatc acaaatgtgc gaatatttgt
     2221 agggacatta cctgacgaca gcgaggccag tactggcgcg gcctgcagcg agatttacca
     2281 ctttgagagt aattttttta actacgttta ttgatctaac tcacgaaaat atcttcggac
     2341 tctggaaatt ggtgtgataa ctttgtcagc atcgcaccat aagcaagcta gctcactcgt
     2401 tgagaggaag acgaaaatga ctccgtttat gactgaagat ttcctgttag ataccgaatt
     2461 tgcccgccgt ctgtatcacg actacgcaaa agaccagccg attttcgatt accattgcca
     2521 tttgccgccg cagcagattg cggaagacta tcgttttaaa aacctgtatg acatctggtt
     2581 gaagggcgat cactacaaat ggcgcgctat gcgtaccaac ggtgtggctg agcgtctgtg
     2641 tactggtgat gcgtctgacc gtgaaaaatt tgacgcctgg gcggcgactg ttccgcacac
     2701 tatcggcaac ccgttatacc actggacgca cttcgaactg cgtcgtccgt tcggtatcac
     2761 tggcaaattg ctttctccgt caactgccga tgaaatctgg aacgaatgta acgaactgct
     2821 ggcgcaggat aacttctccg cgcgcggcat catgcagcag atgaacgtga aaatggtcgg
     2881 caccaccgat gacccgatcg attctctgga gcatcacgca gagatcgcca aagacggctc
     2941 tttcaccatc aaagtgctgc cgagctggcg tccggacaaa gccttcaata tcgaacaggc
     3001 gacctttaac gactacatgg cgaagctggg cgaagtttcc gataccgaca ttcgccgctt
     3061 tgctgacctg caaactgcct tgaccaaacg tctggatcac ttcgccgctc acggctgtaa
     3121 agtgtctgac catgcgctgg acgtggtgat gtttgctgaa gcgaacgaag cggaactcga
     3181 cagcattctg gcgcgccgtc tggctggcga aaccctgagc gagcacgaag tggcacagtt
     3241 caaaactgcg gtactggtgt tccttggtgc cgaatatgca cgtcgcggct gggtacagca
     3301 gtaccacatt ggcgcgctgc gtaataacaa cctgcgtcag ttcaaactgc tggggccgga
     3361 tgtaggcttt gactccatca acgaccgtcc gatggcggaa gagttgtcta agctgctgag
     3421 caagcagaac gaagaaaacc tgctgccgaa aaccattctc tactgcctga acccgcgcga
     3481 taacgaagtg ctgggcacca tgatcggtaa cttccagggc gaaggtatgc cgggcaaaat
     3541 gcagttcggt tccggctggt ggtttaacga tcagaaagac ggtatggaac gtcagatgac
     3601 ccaactggcg cagctcggtc tgctgagccg ttttgtcggt atgctgactg acagccgtag
     3661 cttcctgtca tacacccgtc acgaatactt ccgccgcatt ctgtgccaga tgatcggtcg
     3721 ctgggtggaa gcgggcgaag caccggcgga catcaacctg ctgggcgaga tggtgaaaaa
     3781 tatttgcttt aacaatgcgc gtgactactt cgccattgaa ctgaactaag gtctggggtt
     3841 gatatgcaat acatcaagat ccatgcgctg gataacgtcg cggtcgcttt agcagatttg
     3901 gctgaaggca cagaagtcag tgtcgataac cagactgtta cgctgcgcca ggatgttgct
     3961 cgtggacata aatttgcgtt aacggatatc gcaaaagggg ccaacgtcat caaatatggc
     4021 ctgccgattg gttatgcatt ggcggatatt gcggcggggg aacacgtcca cgcccacaat
     4081 acgcgcacga atctgagcga tctggatcag tatcgctatc aacctgattt tcaggatctg
     4141 cctgcgcaag cggcagatcc tgaagtgcag atctatcgtc gcgctaacgg cgatgtcggg
     4201 gtgcgtaacg agctgtggat cctgccaacc gtgggctgtg tcaacggcat cgcgcggcaa
     4261 atccagaacc gtttcctgaa agagaccaac aacgccgaag gtaccgacgg cgtgttcctc
     4321 ttcagccaca cctacggctg ctcacaactg ggcgacgatc acatcaacac ccgcaccatg
     4381 ctgcaaaaca tggtgcgcca cccgaacgcg ggcgcagtgc tggtgattgg tctgggctgt
     4441 gaaaacaacc aggttgccgc attccgtgaa acgttgggcg atatcgatcc tgaacgcgtt
     4501 catttcatga tctgccaaca gcaggatgat gagatcgaag ccgggatcga gcatttgcat
     4561 cagctgtata acgtgatgcg caacgataaa cgcgagccag gaaaactcag cgaactgaag
     4621 tttggtctgg agtgcggtgg ttctgacggt ctttctggta ttactgctaa cccgatgctg
     4681 gggcgtttct ctgactacgt gattgctaac ggcggtacta ccgtactgac cgaagtgccg
     4741 gagatgtttg gcgcagagca gttgctgatg gaccattgcc gcgacgaagc aacgtttgaa
     4801 aaactggtca ccatggtcaa cgacttcaaa cagtacttta ttgcccatga tcagccgatc
     4861 tacgaaaacc catcaccggg gaacaaagcg ggcggtatca ccacgctgga agacaaatca
     4921 cttggctgta cccagaaagc gggttccagt gtcgtggttg acgtgctgcg ttacggcgag
     4981 cgtctgaaaa cgccagggct gaacttgtta agtgcgccgg gtaacgatgc cgtagcgacc
     5041 agcgccctgg cgggtgcggg ctgccatatg gtgctgttca gtactggtcg cggcacgcca
     5101 tacggtggat ttgtgccgac ggtgaaaatc gccaccaaca gtgaactggc ggcgaagaaa
     5161 aaacactgga tcgactttga cgcgggtcag ctgatccacg gtaaagcgat gccgcagttg
     5221 ctggaagaat ttatcgacac catcgttgag tttgccaacg gtaagcaaac ctgcaacgag
     5281 cgtaacgact tccgcgaact ggcgatcttc aaaagcggcg taacgctata aagtcgaaat
     5341 gagttgttaa taaaaaacgg cgtttcataa tgtgaagcgc cgtttgcttt tccatacctg
     5401 ctaaggattt atcatgaccg cgtattggct ggcccagggc gtgggtgtca tcgcctttct
     5461 gattggtatc acaacatttt tcaatcgtga cgaacgtcgc ttcaaaaagc agctttcggt
     5521 ctatagcgcc gttattggcg tacatttttt tcttctgggc acctatcccg ctggtgccag
     5581 tgccatcctt aatgccattc gtacattgat taccttacgc acgcgcagct tatgggtaat
     5641 ggcgattttt attgtgctga ctggcggaat tggcctcgcg aagttccatc atcctgtcga
     5701 actattgccg gttatcggta cgattgtcag tacctgggcg ctgttccgct gtaaagggct
     5761 caccatgcgc tgcgtgatgt ggttttcaac gtgttgctgg gtgattcaca acttctgggc
     5821 ggggtcgata ggcggcacga tgattgaggg tagttttctg cttatgaatg gcctgaatat
     5881 cattcgtttc tggcggatgc agaaaagggg aattgatccg tttaaagtag agaaaacccc
     5941 tcccgccata gacgaaaggg gttaaacaat taattacgca gggcgctatt tgccagacga
     6001 tcgtcttccg cctggcaagc tgccgcagtg aacagcacgt cagttgaaga gttcagcgcg
     6061 gtttcgcaag agtcctgcaa tacgccgata ataaagccga cggcaaccac ctgcatggcg
     6121 atatcgttcg aaataccgaa catattacag gccagtggga tcagcagcaa agaacccccc
     6181 gccacgccgg atgcgccaca ggcacacaga gaagccacta cgctcaacag cagcgctgtg
     6241 ggcagatcga ccggaatacc cagcgtatta accgcagcca gcgtcaacac ggtaatggtg
     6301 attgctgcgc ccgccatatt gatggtggct cccagcggaa tagaaactga ataggtatcg
     6361 cgatccagat tcagcttttc acacagcgcc atattcaccg gaatgttcgc tgcagagctg
     6421 cgggtgaaga aggcatacac accgctttcg cgcaggcaca gcagcaccag cgggaacggg
     6481 ttacgacgaa ttttccacca caccagcaat gggttaacca ccagcgccac cagtaacata
     6541 cagccaacca gcacgaccag cagttgcgcg tagccccaca gtgtggagaa accggtggtt
     6601 gccagggtag aagaaaccag cccaaaaata ccgatcggtg cgaagcgaat gaccagtttc
     6661 accataaagg taacggcatt cgacatatca ttgaccaggt ttttggtggt ctcgttaccg
     6721 tgacgcagtg cgaagccgag gccgatcgcc cacaccagaa tcccgatgta gttacctttc
     6781 agcagggcgt cgatggggtt ggaaaccatg ctcattacca gtccgcgcat cacttcgaca
     6841 atgcctgacg gcggcgaaat atcacccgcg ctactggaca agtgcagggt agaagggaag
     6901 gcaaagctga agactactgc ggccagagct gcagagaagg tgcccagcag atagaggaac
     6961 aaaataggac ggatattggt tttctgcccg tgctggtggt tagcaataga tgccattacc
     7021 agcatcaaca ccaggatggg ggcaacagct ttcagtgcgc cgacgaacaa agtacctaac
     7081 agaccaacag cttccgccgc gggttttgag atccatgcca gaagaatccc cagaacaagg
     7141 ccgaccagga tttgttttac caggctgcca tgagccagac gccggaatag ccccggtgaa
     7201 cgttgcgtag tcatttttcg atcctttcat tgtgttgtcg cacatccctg gtgcgttctg
     7261 tcgacgcatc tttcaggatg taacaaagtg tttgcaaggt cgagtataag gaaagttaca
     7321 gcttcgggaa gcgaaaaatg ctggatttta aagagaggtc atgttttgca acttaatggt
     7381 tgcatttatt taacataaac agagggaagg actggctatt aaggctgatt agataacata
     7441 tgggcaaaac tatttggaat atgagaacgg ttgcgctcct ttaacgtgtt atgccatata
     7501 aagtcactgg cgcagggagc gcgcaggggg cggccaatcg ccgccgcccc ctgccccccc
     7561 aggctctggc gaacaaaatc gccgctgcgc ggttccctca gcctatccct tgcggctacc
     7621 gggtcgggcg caaggtaacg tccctgtaag atgcgccctc gccccggcct ccagggaacg
     7681 cttcggcgat tttgacgcca ccaacacccg tgcggtcttt ttgtaaagag ctataagccg
     7741 attacataat taatagctca cctttgtctg gaatgagtta tctctaggaa tggcttaata
     7801 tacagcgaat cattctcccc gctgcttatc atgccgataa ttcacccagg cgttgataat
     7861 aaacgtcatc accagaatgc caaacaccac gcccagcgag acggcgattg gaatatggta
     7921 gaagtcgaca atcagcatct tgataccgat aaacaccaga atcaccgcca ggccatattt
     7981 gagcatcgag aaacgctctg ctacgcccgc cagcaggaaa tacatcgcac gcaggccgag
     8041 gattgcaaac aggtttgagg tcagcacaat gaacgggtca gtggtcacgg cgaagatagc
     8101 cggaatgcta tccacggcaa aaatcacgtc gctcaattcc accagaatca gtaccagcat
     8161 cagcggtgtg gcgtacagca atccattctt acgcacaaag aaatgctcgt tgtcgatggt
     8221 gtcggtcatg cgcaaatgac cgcgtagcca gcgcaccagc ggcttgtcgc caatgcctga
     8281 ttcatcttca tgggcgagcg ccatcttcac gccggtaaac agcaggaagg caccgaagat
     8341 atacaggatc cagtcgaact gcgaaatcag ccagctgcct gtgaagatca tgatggtacg
     8401 cagaacaatc gccccgagca cgccatacac cagcacgcga cgttgtaatg ccgccggaac
     8461 ggagaaatag ctgaacaaca tcagccagac aaagacgtta tcgaccgcca gcgatttctc
     8521 aatcagataa cccgtgagaa aggccagggc ctgtggatcg gcaacggcgc gaccttcggt
     8581 ttgcaccaga taccaccaga aagcggcgtt aaacagtaac gacagcgtca cccagaccag
     8641 cgaccaggcc gcagcctgtt tcatggtcat ggcatgtgcc ccacgacgcc cctgcaacaa
     8701 caggtcgata gccagcataa tggcgacaac aacagcaaat ccgccccata gcaacggcgt
     8761 gccgacagta ttcatagaag ttccttacac ataaaaaaac ggccaacgtc ggaagacgtc
     8821 agccgctgct ttttatgcat agacctcgcc ttccggcaag gtctcactta caacaaaaaa
     8881 ggaatacgtc tgcgtattcc tttcgggttg cccggtgacc ggatgtggtt tttcacacat
     8941 cgtaatgacg atcgaccggc aatgaagtta ctcccctttg caggtaacaa agtatgacag
     9001 accattcagt cagtcaatgg tctgttacat accttttaca ctgttttact ttggcaagta
     9061 tagttttacg ctatctgccg gaaaaatcac cccagtctgg cggcggatct cggtcagcag
     9121 tttggctgtg atgcgactga ccgccagccc tggatgatcc accagatgct catccaccag
     9181 ggtagcgaac agctctgctt cgtagagcat ggtattaata tgctgcggct gggtgagatc
     9241 ctgcatctgg ctgccacgcg gcacaaagca gactttctag cattcagaca gtttttcaat
     9301 caccagcgat cccgcttcgc cctgaatctc gcttcccagg acagaatcac tgacctttga
     9361 gtgctgcaag gtgacgctga aatcaccgta atccatcacc accacgccgt gggcgtcaac
     9421 gccgcttgcc agcaaactcg ccgttgcctg cacgcttttc ggctcaccaa ataacgccac
     9481 cgccgacgcc aggcagtaaa agccgatatc cataattgaa ccgttagaga atgccggatt
     9541 aaaggtgttg ggattctcac cgtccaggta acgttgatac cgcgaggaat actggcaata
     9601 gttgaaaaag actttacgca gtttgcctgc tttcggcagc gcctggcgca acaaatgaaa
     9661 gttcggcagg caggcggttt taaatgcctc aaacagcacc acctgatttt cgcgcgcaca
     9721 ggcaatggcg gcatccactt ccgccagatt cgacgccagc ggtttttcgc aaatcacatg
     9781 aattttatgg ctaaggaaaa gttgtgtctg ggaaaagtgc agggaattcg ggctggcaat
     9841 atacaccgcg tcaatggcat cgctttccgc catcgcttcc agcgacgtaa acagatgctc
     9901 gacagaaaaa tcattggcga agtgctgagc ctgctcaagg ctgcgggaat atacggcggt
     9961 taacttgtat ttaccgctct catgggcggc ctcgacgaac tggcgagtga tccagttcgt
    10021 accaatcaca gcgaaacgta tcataacgcg tggatactcc ataaagggat tctgtcgtca
    10081 atgtagcacg cctttttcat cttcgacgtg cgcagatacg cattattctt tcgccggaca
    10141 gccatgctca ctaaacggcc caatcatgac cgatggcccg agatatttcg gctggcgatg
    10201 gaggattttt gcgtcaatca ccgcgctgac acgagcgagt ttctcccgca gttgagtata
    10261 aaaaccatgc ttcatattga ggtctggcgc gttaaacccg atagcgtcga tgccgtactg
    10321 ttttgccagc cagatggcgc gttggttatg aaattcttgc gagataatgg tgatatggtt
    10381 ttccccaaac acctttttgg cacgcactac cgaatccagc gttgagaatc cggcatagtc
    10441 gcaaaagatc actttcgccg gtacgccttt agcgatcaaa gcctgctgca tacctgatgc
    10501 ttcatcgtaa tttttacgtc cgttatcgcc actcaccagt agccatttca cttttccggc
    10561 gtggtacaac gctgctgcgg tatcaatacg tcgggtaaag tagcgattac ccggcctcgc
    10621 ccccagcaat aaacccacgt tacgcgccgg gacggcgttg acatcgcccc aggtcagctg
    10681 cttgctggcg ttgaccataa cgcgatcggc gataaaaatg gtcgctcctg ccacaagtaa
    10741 cagtaagcag gcaatcttta gagtccggcg agaaaaacaa atacggagaa gaaggcgggc
    10801 aaatgcgcgc agcatggtcg ttatccttaa cgaaaggggg gaggtcgatt ttaggcgcta
    10861 aagaaagaaa agataacgcc tgacatcatt ttcagatttt gtcctgccca agcgacaact
    10921 gcgttagcca cagccgggtg tcgaactcaa gctggtggta ctgcggctcc atatggcagc
    10981 acaattgata gaacgctttg ttatgctctt tctccttcag gtgcgccagc tcgtgcacca
    11041 cgatcatgcg caaaaacggt tccggcgcgt tgcgaaacac ggtagcgacg cggatctctg
    11101 cttttgcttt cagcttgccg ccctgcatac gagaaacagc ggtatgtaac ccgagcgcgt
    11161 tttttagcac atggatctta ttgtcataca tcactttatt gatcggcggg gcattacgca
    11221 gaaactgatt tttcagatcc tgagtatatt gccagagagc tttatcggtg gcgtagtcgt
    11281 gtgttcccgg atagcgtttt gccagcacat cgcccagacg ctgttcgtta atcagtgttc
    11341 gcacctgcga aagcagctgc tcgggataac cctgaagata agtaagattg ctcataaccg
    11401 cccacgaaaa agagaaaaag ggtatactca cgcacccttt tcaggggcaa cgtcgaaatt
    11461 ttatcattca ggagggccga tgagccactt agacaacggt ttccgttcac tgacactaca
    11521 acgttttccg gcgacggatg acgttaaccc gctacaggcg tgggaagcgg cagatgaata
    11581 tttgctgcaa cagttggacg acacagaaat ccccggcccg gtgttgatcc tgaatgatgc
    11641 ctttggtgcg ttaagctgcg cgctggcgga acataagccg tacagcattg gcgactcata
    11701 catcagtgaa ctggcgacgc gcgagaattt acgcctaaac gggattgatg agtcgagcgt
    11761 gaagtttctc gacagcaccg ccgactaccc gcaacagccg ggtgtggtgc tgatcaaagt
    11821 gccgaaaaca ctggcattgc tggaacagca actgcgtgcg ctgcgcaaag tggtcacgtc
    11881 ggatacacgt attattgccg gtgccaaagc ccgtgacatt cacacttcca cgctggaact
    11941 gttcgaaaaa gtgctcggcc cgaccaccac cacgctggca tggaaaaaag cgcgcctgat
    12001 taattgcacg ttcaatgaac cgccgctggc cgatgcgccg cagaccgtta gctggaagct
    12061 ggaaggtact gactggacta tccataacca tgcgaatgtc ttctcccgca ccgggcttga
    12121 tatcggcgcg cgcttcttta tgcaacatct gccagagaat ctcgaagggg agattgtcga
    12181 tctcggttgc ggtaatggcg ttattggtct gacgctgctt gataaaaacc cgcaggcgaa
    12241 agtggtgttt gtcgatgaat cgccgatggc ggttgcttcc agccgtttga acgttgaaac
    12301 caacatgcca gaggcgttgg atcgcagcga gtttatgatc aacaacgcgc tctccggcgt
    12361 ggagcctttc cgctttaatg ctgtgctctg caaccctccg tttcaccaac agcatgcgct
    12421 gaccgataac gtcgcctggg agatgttcca ccacgcccgc cgctgcctga aaatcaacgg
    12481 cgagctgtat atcgttgcca accgccatct ggattacttc cataaactga agaaaatttt
    12541 cggcaactgc accactattg cgacgaataa taaatttgtg gtgctgaaag cggtgaagct
    12601 gggacgtcgt cggtaagatt gggagttacc tgccggatgc ggcgtaaacg ccttatccgg
    12661 cctacgttca gcacgttacc cccaggccgg ataagacgcg gcaagcgtcg catccggcaa
    12721 tgtctgctca gttctggaat cgcgcttcca taaaacaaaa aatccctgtc accatcctct
    12781 acattctctt gtttagcgtt tttctacgtt tattcttccg tcacacagat aaattcatcc
    12841 gttgccaatc tgtcaacgga tgttatcata ttgccaattt gtcaacggac gtgatgaaga
    12901 atgcacctga taactcaaaa agcattgaaa gatgctgcgg aaaaataccc gcaacataaa
    12961 acggagttgg tggctctggg gaacacgatt gctaagggat atttcaaaaa acctgagtca
    13021 ttaaaagcag tattcccatc tctggataac ttcaaatatc tggataagca ttatgttttc
    13081 aatgttgggg gcaatgaatt acgtgttgta gcaatggtct tttttgaatc gcaaaagtgc
    13141 tacatacgtg aagttatgac gcataaagaa tacgatttct ttaccgctgt tcatcgtact
    13201 aaggggaaaa aatgattgcg attgccgaca tcttgcaagc aggagaaaag ctaactgctg
    13261 tggcaccttt tctggcgggt attcagaacg aggaacaata cacccaggcg ctggaactgg
    13321 tagatcatct gctgctcaac gatcctgaaa accccttgct ggatctggtg tgtgccaaaa
    13381 taaccgcgtg ggaagaatca gcgcccgaat ttgcggaatt taatgccatg gctcaagcca
    13441 tgcctggcgg tatcgccgtg attcgtaccc ttatggatca atatggttta accctttccg
    13501 atctgccgga aattggcagt aaatctatgg tgtcacgcgt tttgagcggg aagaggaaat
    13561 taacgctgga acacgctaaa aaattggcaa cgcgattcgg catttctccc gccttgttta
    13621 ttgattaacg cgacgtgccg gatgcgttgc atccggcgct tcaaataacg atttaaatct
    13681 ccagcgccag ccgtgttccc tgggcaattg cccgtcgtgc gtccagctcc atagccacat
    13741 cgcagccgcc aattaaatgc acagttttcc cgctatcaat cagcggttgc gccagcgcgc
    13801 ggtttggctc ttgccctgcg cagatcacca cattgtccac tgctaatacc tgcgtttcgc
    13861 cgttgatcac cacatgcagc ccgtcatcgt caatcttctg ataacttacg cctgggatca
    13921 ttttcacacc ccgcgagagc agggtggtgc gatggatcca gccggtggtt ttgcctaacc
    13981 cctgtcccgg tttgctggct ttgcgctgga gcatcacaat ttgccgtggg ctacgcggga
    14041 tctgcattcc ctgcgggctt aagccaccag cctgttgtag gctgctgtcg atcccccact
    14101 cattacagaa cctggcgata ttctggctgg tggattcgcc cggctgactt aaatacatcg
    14161 ccgtatcaaa accaatcccg ccacaaccga tgatggcaac cttgttgcct accggcgctt
    14221 tgtcgcgcag tacatcgaga taactcaata ccttcggatg atcgatcccg tcgataggcg
    14281 gaacacgcgg cacgatccca ctggcgagga tcgtttcatc gaacgcctgt aactgatccg
    14341 ccgtcacggt gtgattgagt ttcagcgtca cgcccgtcac ttcgatcatc cggcggtagt
    14401 agcgcagcgt ttcgtaaaac tcctctttgc cggggatctg tttggcgata ttaaactgcc
    14461 cgccgatctc gctgtgcgca tcaaacaatg ttacctgatg cccacgcgcc gccgcgttaa
    14521 tggcaaacgc cagcccagca ggtccagcgc cgaccaccgc cagatttttt ttctgcacgg
    14581 cgggaaggat tggcattttg gtttcgtggc aggcgcgagg attcaccagg cacgaggtga
    14641 ctttgccaac gaagatctga tcgaggcagg cctggttgca gccaatacaa gtgttgatct
    14701 catcggctcg ccctgattgc gcttttgaca gcagttccgc atccgcgaga aacggtcgcg
    14761 ccatcgatac tatatcggca tcgccgcgcg agagaatatc gtcggcaacc tgcggatcgt
    14821 taatccggtt ggtggttacc agcggcagcg agacgtggcc tttcagtttg cgcgtgaccc
    14881 agctaaatgc gccgcgcggc acgggcgtgg cgatggtcgg aatccgcgct tcgtgccagc
    14941 ctattccggt gttgataatg gtcgcgcccg ccgcttcaat ggcctgcgcc agttctaccg
    15001 tttcggcaaa agtgccgccg ccttcgacca ggtcgagcat cgacaaccgg taaataataa
    15061 tgaagtcgtt gccgacgcgt tcgcgcaccg tacgcactac ttctacggca aatcgcatcc
    15121 ggttgcggta atcgccgccc cactggtcac tacgctgatt ggtgcgcagc gtcagaaatt
    15181 cgttgatcaa atacccttca gagcccatca cttcgacgcc atcgtatccg gcctcccgcg
    15241 ccagttgcgc gcagcgggcg aaatcgtcga tcagttgcag gatctcttca tgggtgagtt
    15301 cgtgaggaac gaaacggttg atgggggcct gcaatgcgga cggtgcgacc agatgcggtt
    15361 ggtagctgta gcgcccggta tgcaaaattt gcagggcgat tttgccgcct tcctgatgta
    15421 cggcttcggt aatggtgcga tggtgtggga tctggctggc gtcgttgagc attgcgccac
    15481 cttccatgcc aacgcctatt aaatctggtg caatgccgcc gctgacaatc agcgccacgc
    15541 cgtgacgggc gcgttcggca taaaacgcag ccagccgttc agcaccgtcc gggtattcct
    15601 ccagcccggt gtgcattgaa cccatcaaca cgcggttttt taacgtggta aaacctaaat
    15661 ccagcggggc gaacagcgac gggtagctca taaaattgtc cagtatgtaa aataattgtt
    15721 acggatgagt tctaatttag ccgtcgatgg agaaaaggga aaaggggagt gcggggtttg
    15781 tgatgggatt caaaaagaat acgcccggtg gtaacgccgg atgcgacgct tgccgtgttt
    15841 tatccggctt gggggattgc acatgtaggg cggataaggc gttcacgccg catccgccag
    15901 ccgttgcaaa ttactgctta cggaaaaaat cgttatacaa catatacaaa tgagcggcac
    15961 tccaggagaa atttggtgcg ccttgctgcg cgcctgtcag cgggttgtaa ttctcctgaa
    16021 ttgggccatc ggcggttaat cctttggcgt gcctgaagaa cgtatccgcc agcttcaggg
    16081 catcatcacg ataaccgtaa cgctccatcc ctttcagacc aaaccagaac tgatccaccc
    16141 atacgcgccc gcgccagtag atatcagcgc caaaagccgg attggttaac gccgccgttc
    16201 ccagcggaac aaaggtgttg aactctttag gatcgagcat caccttcacc accgcgtcgg
    16261 cattggcctg cgttgccgca ccgttaaaca gcggcgacca gccttccggc cctttaccgc
    16321 gctcaacaat cggtttgccc gcgcagccgt tcgccagtgg tttatcttca atacgtacgt
    16381 catagtagaa ctgggtagtc gggtcgaaca tacaggtgtt gatgtagtcc gcgagctgct
    16441 gtgccaactg gcgatagcgt ttggcctctt ctggcttacc gaggatggtt gccatctccg
    16501 ccagataatg gttatcgctg tacatatagc tggcctgatc caccgactcc tgcaatagcg
    16561 agtagcccag caacgttccg tcctgactgc ggttttcggc gaatttcacc gtctagtcgc
    16621 tacgtttgcc gccattagcg acatatttat ccaactgttc tttgtcgata aaaccaaaga
    16681 cggcggcatc atcacgcccc gattcccacg acgcagcgac ctgtgccgga atttccagac
    16741 tgtcgtactg gcctttctcc accacgcggg cgtagttgtt cagcccagac tgcgtctctt
    16801 ctttatcgcc ttttttcacc gtaaacagca tctcgccgct ctcggtgttg tgggctttgt
    16861 cgcgggtcgc gccatattcc ggcaccccgt tgccgttatg atcgcggtta cgtaaccacc
    16921 agtcgtgata ggccaccagt ttcgggtaca tctctgccaa ccaggcttta tcctgggtga
    16981 cgttgtatac ttccatcacc gaccaggcgg caaggctggg tttggtatta cgttcgttcc
    17041 agttgccgcc atcgccacca tgctcggggc taaggttcca cgctatcagg tcggggacaa
    17101 agcccacatc ctgcggacgc acgctgtcgc caggctggat ctgccaggag aagaccgcgc
    17161 ggatattctc tttggcgata tccggattga aatgcgccat cgcaaacgcc tgcttccagg
    17221 tatcccacgg ccaggtctga ttgccggaga accagcgtcc ggtcaccgac ggcgtgacgg
    17281 tgttataatt caccgcaccg cccggtgagc gccagttacc gttgagcgtt tcgatcgctt
    17341 tcaccgcgac gcgcgtctgt tccggcgtcg catccggatt ggttaaccct ttcttcaggt
    17401 attcttccca gcgttgctgc gaggcggtga gataaaacgc cggacgcgcc agaatatcgc
    17461 ggatctgcat ttgctctttg ctaacttcct gagcggtcag cagatgggaa taggtggtgt
    17521 agagcgtggt cgaaccgttg atatgcgcct tactggtaaa gcgattaccg ttgatttcag
    17581 tctgcaccgg cagggattta tgcacctgat attccgattc gccggaggtc agcagatccc
    17641 aggtggcgcg cactttgcca aaggtgactt tcaggccatc acgggtggcg ctgattttgc
    17701 gctgatagtc agggtattcg ccagcaatgg ttttatcgga aagcggtttc ccttctttcg
    17761 cttccagttt ttccagcagt tcgccatccc acaccagatc cagcggttta ttgctgatga
    17821 ttttagtttc cagtagtgac gtgcgcggcg tggcgaagcg cagagtcatt tcgacctgca
    17881 catcttttgc tgtcagtttt tgcaccagcg cgccgggaat actgtatgcc tccagcgtga
    17941 agttgacttt cttgccgtct tgccagacag tcaggcggtc gaaattgctg gccataaagt
    18001 tgatgtattc ttccgtcagc agcgcaacgc ccggaaagcc gcccatggtg ttcgggccgt
    18061 ctggcaacag atgaccatgc caggctccga gatcgaaaaa cggattaaaa cgctggtgat
    18121 cgtcgtaatc gtaatccttc atgtactgcg gcgcaccagt acggttaatc acgtttttat
    18181 aattgtcggc gttagcggca tgagcggaaa aacttatcag cagagcgcag gttactggcg
    18241 ttaaaatagt tttgattttc atgaaattac agtccttatt accagtagaa atattgcatc
    18301 aggaagattt ggtcgctggg atcgttagag gcatttttca tgtagaagcg gctatcaaaa
    18361 gccgtggcga aacgtccgcc gccatattcg taccagatac caaactgcat gaaagaggtg
    18421 gtttcatcgt cagcgtggtc agggcgatgt ttggcgtagg tgtaagcggt ggagagataa
    18481 acgcccttta acgcttcata catggcgctc accatcatcc cgctttcact tcccggtacg
    18541 tccagcccgt cggcacgtcc ggtatggtgc caggcgcgag cggcaaagtt aggggtaaac
    18601 aggcccgtga gatagagttg tcctgtgccg tcggtaattt ccagaccatt catccaggtg
    18661 atgccttcgg caaggtcata ctggatgtaa ccgttcatcc attgccggat aggtgtattt
    18721 gtcgtcgtag cgatcgtact tgccaaaatg tagccaggct ttgctttcgt catgatgtcc
    18781 ggcaggtgtg gcggtgacgc tataacgaaa gttgccgctt aagttctgta ccttcagcgc
    18841 ggtcataatg tcgcgggtat tgggaatgac gtagcccaaa tcgggtgtga agtcgcccca
    18901 ccattgcagg tcatccagcg aggaatcctg acgcagactg agcatgattt cggtgccatc
    18961 attggtacga taaccgccat agaaacggtt aatcccacct tcgaaaccac cccagctatg
    19021 gtcaggcacc taggcattac cgtcgtggtc ggcctgcacg gtccagcctt cgccgtaaat
    19081 caggccgaac caggagccgt acttcatctc caggccacct tcaatatagg tgccgtcgct
    19141 gtatttatgt ttattctcac cctctaaatc catatatcca ccaagcccta attcgccgta
    19201 aaaacgaaat gaaggagaag atgtcgaggt aattgcgggt gtttttgtgt cgtcatgttc
    19261 tgccggacgt gcttcttctc ctgccgcaga aaaggcgtag caaaacggca gagcaagtaa
    19321 tgctctgcat ggtgcagtga ttaatttcat tgtgttaatc catgagctaa agaaaaagta
    19381 cagcctttta tcgttgtaat tataaaaata taaaggcaga aactattttc cctctcctca
    19441 tccaggaaga gggaaaatgc ctgctcattt tttattagtc tgcttaatca acattgctgg
    19501 ctggcgtcgc ttcaatatgg cgctcttcag ccgttaatcc ctttatatat ttactgtatt
    19561 tccaccacgc aaagccgagg aagataacaa taccgccgac gttataaaaa atgatggtca
    19621 gaatattcgc gccagtcggg aatgtcgaag cgacaaaccc tacggcaaat atcgcaatca
    19681 gcattgaaac aacgataata ccggtgcggc gggagcccat acggaaatcg cgtggcaggt
    19741 gatctaattt ggcgcgtaaa ttcaggtaag ccagcatgat gaataacggc ggaagcatgg
    19801 acgctgcggc ggtcatatta ataatggtat tcatcagatc ctgcacggta ttggaaccga
    19861 gcatcgggat aatcatcagc gggatgacga tcagaaactg gatccatgct gcgcgcgccg
    19921 gaacgccgtt ttcgttaagt tcgacggttt tcttaccaaa gatgccttcc gggatttcgg
    19981 agaagaaaat tttcaccggc gttgcggtcc acatcagcag ggaaccgaac attgcggtaa
    20041 aggacaccag accgacaaag cgattcatca acgcttccgg cagaccaaaa tacgccgcca
    20101 tgccgtggaa tacctgcacc gatccgccgg taaatttcaa ctctttgctg ctgacgaaga
    20161 cgttaatcag caccgaggag acggaataca gtacgccgat aaaaatcccg gcgaggatga
    20221 tcactttaac gaacgatttc gaaccgcctt tgacgtcgtt aacgtacacc gcgacggact
    20281 ccgcgccacc tgcggccata aagatccagg tggtaacgcc gaggaacgcc cagttgaagt
    20341 tcgggatcat cgcgtcaacg gtgatcggat cggcaggctg tacgtcgcca accagtgccg
    20401 tacctgccag taaaatgtag gagagcgtta acagcagcat cagcgttgaa gtgacggagg
    20461 taattggccc cagcattttc gccccgttgg tggaaaccca ggtggagaag gcgaacagca
    20521 ccatactgat aacggtggtg gcaaccggcg tcataatata ttcgtagccg aggaaggcat
    20581 acgaagcata agcaataacg cgcggcaaca gcgaggtgaa aaagaacaga tttacgaacc
    20641 agtaagtgta ggcagtaata aatgcccaac gtccgcccag cgaacttttt acccacgcgt
    20701 agacaccggc ttctgagttt ttatttaacg aaacaaattc tgcgatgatc agacagaagg
    20761 gaataaaata aaaaatcgtc gcgaggaaaa acatcggtgc cgaggccagt ccaagctcaa
    20821 tattattatt gataacgttg ttaaagctgt aaacggcggc aaaagtcagc gagagcaagc
    20881 cgaatttgcc gattgtatta cgtttggtat cagacataac acatctccga aaatgtgtag
    20941 ggtagcggtg gcggcaaact ccttttgccg ccgtagttgt tatttgttat ggaaataacc
    21001 atcttcgatg gtgactttga gaaccacttt tttgaccgcg ttattgcaga taaaccgata
    21061 cgcttcatgg atatcgcaaa taacgatttg cccttcgtgg acctctacgg tttctccgca
    21121 gccttttaaa tattcacggt cagtttcatc acgataatat tccactacct gtaatgtctc
    21181 tttcggcgca tattcaattt tttgctgccc ttgcaggtag taatgcactt caaaatagcg
    21241 tcgatgcccg gtaaatagcg catgggtcgc ggagtcattt tccacacggt aggtcaatga
    21301 atcaccgatg gagtgggcga cgccaggctg aatgttgtcg atattttcaa tcgcttcaac
    21361 gcagcgttgc cacttcttgc cagaggcgta aatctggcgg aactgttcta agttatcgat
    21421 gatcctcatt gcttattctc gctgtgcaaa ttcgtggaaa agaaccctgc gccgaactca
    21481 tacgacgcca ggctttgcgc ggtagcttct ccgccagaaa ccggcagcaa cgtaaagccg
    21541 tagctgaagt cacggaacca gacgcgccag gagtccagca cctcgctgcc ccaggagttg
    21601 gagccgaggc caagtagctg gtggtcgagg ttcagggtga tgtcatcact gcgctgcagc
    21661 tcgttacagt gctgggcagc gtggatgttt tcctgggtat agtgccaggc gctgaagtta
    21721 attgggcgct gcggaaccac cagcagaccg ttgtcgtggc ggttagtcag tgccgtccag
    21781 cggacatgct gacggttgcc gttgttctgc gggaagggat agttctcgaa catggcatcg
    21841 acggtgctgc gccagatatc gatgatgtta gcctgctggc tgtcggcgta gttttcgccc
    21901 ggtccacgac cgtaatacgc cacctgatcg tattcgccgt taatccccat ggtgaaaccg
    21961 atgcacggaa tgatgtgcgg atagtcgccg taacgctcgc cggaaagcgc cacgttaacc
    22021 tggccatcgg cagcgatgcg ccagatgtag gtacagcgca tcccgaagtc aaacaccggc
    22081 ggtgcaataa ctgtgcggct gatgatcaac acttcaccat cgctctgttc tacggcaaag
    22141 tcgcgcagat gttcctgcat gatctgcaaa tgattcggct gccacaaccc ttcgtactcc
    22201 tgcttgtggt tgtcgatcat cggcttgaag aagttgatct ttggctcgcg ggtcagcagc
    22261 gactcgccat ttacctgcca ggatgtcggt ttgccactca tttttgagaa ggtgatcgcg
    22321 aagttgtagc cgcgaacggt gcagctcaaa cgatcgtctt ccagcgtcag cggacgcgca
    22381 ttatttggtg cgaaaggcac tggctgcgcg gtgttttcct tcagcgggaa ctgataagtg
    22441 gcgatagaat gcccggcttc gctgtagcgg gtgcgggaat ctttggtcac cgtaatgttg
    22501 aggaacgctt cgcgggcgtc cagctgcggc agcgtgatct gcaagggggc ttcgctgttc
    22561 ggcgcaacgt cgcgcagttt aatctgctgc gtcgcgagcg tttcaccttc ggcgcgcacc
    22621 tctgcgtgca gggtgtagtc atcaagcgtg gtaaaccaca gtttattttc gactttcaac
    22681 tcgccgcgag tcagcgcgtg gatttttacc ggcgcgataa cctgtttgta ctctttcagg
    22741 cccgatcccg gcgtctgatc ggaatagatc aaaccatcaa gacagaagtt atagttgttg
    22801 ggatagtcgc cgtagtcgcc gccgaattta taccagacat tgccgtggtc gtcctgtgcc
    22861 tggatcccgt ggtcgcacca ctcccagaca taatgaccct gaatgcaatc gtgcttatag
    22921 aagacgttct ggtactccgt cagcccgccc ggtccgttcc ccatcgcatg agcatattca
    22981 cagatgatgc gcggcttcgg atgcgggtat tcaccaaact cattcatcag cggcacgcgg
    23041 gtgtacatgg tggaaataat atcgaccact tcagcatcgc gatcttcttc gtaatgcacc
    23101 agtcgcgtgt catccagcgc tttcgccgca tggtacatcg cgcggatgtt acagccatag
    23161 ccggattcat tgcccagcga ccagatgatg atcgacggat ggtttttctg tgcgtggata
    23221 tggcgaacaa tgcgctcgac gtagactttt tcccactgcg gatcgtcggt aatacggctg
    23281 atatcgccga cattagcaaa gccgtgcgat tcgacgtcgg tttccgccat cacaaacagg
    23341 ccgtagatat cacacaattc gtaaaaacgc ggatcgttcg ggtagtgagc ggtacgcacg
    23401 gagttgatat tgtgctgctt catcaactgg agatctttct cgacgcgatc cattccaacg
    23461 gcgcggcctt tgcgatgatc gttgtcgtga cggttgacgc cgtgcagcat cacataacgg
    23521 ttattgatcc agaacagacc gtcgcgcact ttgatatcac ggaagccaac gcgttgtggc
    23581 accacttcca gaacgttgcc gttggcgtct ttcagcgtca tgaccagatg gtaaagataa
    23641 ggggattctg ctgaccattg ctgcggctgt tcgacagtaa aagcaaagct ggcgctggtc
    23701 agtttttcaa ttgccaaatg atcaatggcg ctgctgtgca ccacgcgttc gccatcaaac
    23761 agggtatatt ccagcgtcgt gacgacaggg gaggcggcga gattttccag caccacttcg
    23821 caggaaagcg tggcatcgca ataggcttcg tcaaagtcgg tacgcacagt gaaatcgtta
    23881 atatgcgtta ggtgttttcc gaccagataa acatcgcgga agatccccgc cgaccaccac
    23941 atatcctggt cttccacgta ggtagagtcc gcccactgca tcacgcgcac acacaacagg
    24001 ttgtcgccgg ttttaaccat cgcgctgatg tcaaactctg cggtcaggcg actgcccttg
    24061 ctgaaaccca catactgacc gttaacgtag acttcaaaat aggtttcgac gccgtcaaat
    24121 ttaatcagcg tctgtttacc ctgccagccg tcgctgaggg tgaaaatacg ttgataggca
    24181 ccggttgggt tatcgctggg gacaaacggc acatcgatgg ggaacggaaa accttcgtcg
    24241 gtatattgca gtttgccgtg accttccatt tgccacatgg cggggacggt aatatgcccc
    24301 cagtcagcca ttaactcaga ggtgaaggct tctggtactt gcagcggatg gtcaaaaaag
    24361 tggaaattcc actgaccgct taagggcaga aacaggctgc tggtttcgcg ggcaaaggta
    24421 cgcgcttgcg caacagaatc atatgaaaaa aagtacgcac gcggcgcaag tcggttttcg
    24481 tgggtgagct gaatgttttc ccagcgattc ataggacctc ccggtagaga gaaatcggaa
    24541 ccattcatgg tgatggtagt ttgtgtaatt gctggggggg ttagtaaaat ttttactaaa
    24601 agtagcaggt aaatgcattt actttaactg gatcacgaaa ataaacggca gcactgtccg
    24661 gcaaaaatga ttgccggaca gtgtgtaagg ggatttaacg ggtcgtgccg cgcagtttta
    24721 atttgctggg aacgaagact agcagcggca gcgcgcgacc atcgcgggct ttttcataca
    24781 ccaggttaac gccctgactt cccatcatct cggaatggat gcgcacggtg gagagcggcg
    24841 gaaaggtaaa tcgcgcggtg gggatatcgt taacgctgat aagcgaaata tcttgtggga
    24901 tgttcaggcc acgttcatga attgcccgca gtacgccgat agcaatggaa tcggaagcaa
    24961 caaacagtgc cttcggatag tcttcccgcg ccagcatttg ttttgccagt tcataacccg
    25021 acgaactgga aaaaccgccg cgccagatat cctcttcgcg taccacttgt ttcagtcggc
    25081 catattccgc aaaggcgacc tcacgaatat ccgccttgcc aggctcatct tcaccgccaa
    25141 taaaaccaat acgattaacg ccctggttga tatagaagtc gatgatttct ttactgatgc
    25201 gtgccagatc gatatccacc gcatcgtaac cgctgccggg ttcgtgaaag tcgataaaac
    25261 agatattgtc ggtcaacgcg ctggcagcgg cgcgcagggc gggcgtgggt ttgccgacaa
    25321 ttaaaatacc ggtgacgttt ttaatgtctg gtaatccgtt gtgttcataa cagttggtca
    25381 gttcgatcgc cagcttttcg cactgagttt caatgccgtg gcggatcgcc agatagtaag
    25441 gatcgttgat ctccagctcc tgctggtagc tgtagatagc cagaatatgg tgttggttga
    25501 ctgcgcctgt ctgtagttta cgggcgctac tggtcttgta ctccagcttt tcggcgatct
    25561 cgagaatgcg atgtttcgtc tcttctttca cattcaatgt cggatcgtca tttaagaccc
    25621 tggatactgt cgccagggat acgccagctt cgattgcgat gtcttttaat gttgccatgt
    25681 ttaccttttt tactttccct gatgtgcgcc cccattgtag aggaagtgct tatcgggtca
    25741 cctgttttca gtaaataaag cgcgtttctt tgacgctgta acggtaacac agcggcttag
    25801 gaatgtgatc acgcctgcat tctccatttt tagcctacag gattgagaaa aacttttgct
    25861 atagtttagt aaaattttac ccgaagggag gaagcaatgg aaaccgtggc ttacgctgat
    25921 tttgcacgtc tggaaatgcg cgtcggaaag attgtggaag tgaaacgcca tgaaaacgcc
    25981 gacaagctgt acatcgtaca ggttgatgtg gggaaaaaac actgcaaacc gtgaccagct
    26041 tggtgccgta ctacagcgaa gaggagctga tggggaaaac ggtggtggtc ttatgcaatc
    26101 tgcaaaaagc gaaaatgcgt ggtgaaacgt cggaatgcat gctgttgtgt gcggaaaccg
    26161 atgacggcag tgaaagcgtg ttgttaacgc cggagcggat gatgcctgcg ggcgtacgcg
    26221 tcgtataaaa agatcggatg gcgacgtcgt atcgccatcc gatttgatat tacgcttctt
    26281 cgacacttac tcgcatggcc gccagcgcct tacgcgccgc tttgatcacc agttcacact
    26341 gttcaatggt cagtgtcagt ggcggttcaa tgcggatcgt tttggcgtta ttgagcgttc
    26401 cggccaccag tacgcgctgg cggaacatct cgctggcaaa gttatagccg atttcgttat
    26461 caacaaactc aatcgccatc aacatccctt taccacgcgc ttcctgtacc agatcgggat
    26521 attcccgcgc cagttgacgg aaaccgtcca gcaacatatc gcctttttgc tcagcctgag
    26581 ccggtaagtt ctgctccagc aacacattga tggtcgccag cgccgccgca caggccagcg
    26641 ggttgccgcc aaaggtggtg gtatgcagga atgggttgtc gaacagaact gaaaacacct
    26701 cttcagtggc gatggtcgcg ccaatcggca tcacgccgcc gccgagggct tttgccagac
    26761 acagaatgtc cggctgaacg ttctcatgtt cgcaggcaaa catcttgccc gtgcgcccca
    26821 tgcccgtttg tacttcatcg aggatcatca gtgcgccgaa ctcatcgcac agcttacgca
    26881 cggcggtgag atagcccggc ggcggcagaa ttacgccacc ttcgccctga atcggttcga
    26941 ggatcaccgc agccacatca tcaccggttt ttttgcactc attaagagcc gtgcgcatgg
    27001 cttcgatatt gccaaacggc acatgacgga accctggcag cagtggcata aacggtttgc
    27061 ggaaggtcga tttcgccgtg gccgacagcg cgccaagtga tttaccgtgg aacgcgccgc
    27121 tggtggcaat aaaagtaaat ttgccgcgcg gcgactgata agccttcgcc agcttcagcg
    27181 ccgcttcaac ggactcggtg ccgctattac agaagaagct gtatttcagt ttaccgggcg
    27241 ttagcgcagc aagggttttc gccagcatag cccgtagcgg atcgagtagc tcctggctgt
    27301 gcagtggttg tttcgcaagt tgattctgta cggcggaaac cacaactgga ttacggtgcc
    27361 ccacgttgaa aattccaaaa cctcccaggc agtcgataaa ctcctgtcct tgggtgtcga
    27421 caagcgtatt taaacctccc gcttgccact ctacggctcc gtaatccccg ccggcggtaa
    27481 cagatttgcg atactctaaa aaccccggat tgacatgctc tttgaagtat tcaatcacct
    27541 ctcggttaag tgctttcatc tcctcatgat ccagcgttcg cttctcaatg agattcaggg
    27601 cgtgggcgct gcacgctaaa gccgatgcgc tcgaaggtaa cctgttcaaa atatgctccg
    27661 gaggctcgcg tatcacatga taccgattta agtattgcag ggattgcgcc actccggctg
    27721 cgatcgcgaa aatcgggata aacacaaatg cggttatcat ttgtgcaata tttaatgcta
    27781 tccgttaaca tttgttgtta ttttgcacgg ttttgtcggg tatggatttg atgattgctg
    27841 cgcaaatgca cgttttcagg gcaataattg cctgattttt gggcaagata attaacgttt
    27901 tcgtatagta acagagtgaa agtattttaa atcaaagaat tagatttgtg ctttaatcgc
    27961 aaattgcgat ctaaatcaaa ttaatcggtt aaagataacc gcagcggggc cgacataaac
    28021 tctgacaaga agttaacaac catataacct gcacaggacg cgaacatgtc ttctcatccg
    28081 tatgtcaccc agcaaaatac gccgctggcg gacgatacca ctctgatgtc cactaccgat
    28141 ctgcaaagct atatcactca tgctaatgac acttttgtgc aggtgagcgg ctttaccttg
    28201 caagagttac aagggcagcc gcacaacatg gtgcgtcacc cggatatgcc aaaagcggcg
    28261 tttgcggata tgtggttcac cctgaaaaaa ggggagccct ggagcggcat cgtgaaaaat
    28321 cgccgcaaaa atggcgacca ttattgggtg cgggccaatg cggtaccgat ggtgcgcgag
    28381 ggaaaaatca gtggctatat gtcgattcgt acccgggcga cggatgaaga gattgcggcg
    28441 gtggagccgc tgtacaaagc gttgaactcc ggacgtacca gtaagcgtat tcataaaggc
    28501 ctggtggtgc gtaaaggctg gctgggtaaa ctgccttcat taccgcttcg ctggcggacg
    28561 cgtggagtga tgaccctgat gtttatcttg ctggcggcca tgctttggtt tgttgctgcc
    28621 ccggtggtga cgtatttcct ctgtgtgtta gtggtattgt tggcaagcgc ttgttttgaa
    28681 tggcagattg tgcgcccgat agaaaatgtc gcccgtcagg cactgaaggt ggcgaccgga
    28741 gagcgtaata gtgttgagca cctgaatcgc agcgatgagc tggggctgac attacgcgcg
    28801 gtagggcagc ttggcctgat gtgccgttgg ctaattaacg atgtctcaag ccaggtgtcc
    28861 agtgtcagaa atggcagtga gacgctggcg aaaggcaccg atgaactgaa cgaacatacc
    28921 cagcagacag ttgataacgt tcagcaaacg gtggcgacca tgaaccaaat ggcggcgtcg
    28981 gtgaaacaga actctgccac ggcgtcggct gccgataaac tttctatcac cgccagtaat
    29041 gcggcagtgc agggtgggga ggcgatgacc acggtgatca agacaatgga cgatatcgcc
    29101 gacagtaccc agcgcattgg caccattact tcgctgatta acgatattgc gtttcagacc
    29161 aatattctgg ccctgaatgc ggcggtggaa gcggcgcgtg ccggcgaaca gggcaaaggt
    29221 tttgcagtgg tggcagggga agtgcgtcat ttagccagcc gcagcgctaa tgctgccaac
    29281 gatattcgca agctgattga tgccagtgct gataaggtgc aatccggttc gcagcaggta
    29341 cacgccgccg gacggacgat ggaagatatt gtggcacagg tgaaaaacgt cacccagttg
    29401 atcgcccaga ttagccattc aacgctggaa caggccgatg ggctttccag cctgacccgt
    29461 gcagtggatg agcttaacct gatcacccag aaaaatgccg agctggtgga agagagtgcg
    29521 caggtgtcgg cgatggtgaa acaccgcgcc agccgactgg aagacgcggt gacggtgctg
    29581 cattaatcgg tatgccggat ccggcggttg gagcacaatg cctgatgcga tgctgacgca
    29641 tcttatcagg cctgcggggt gtgtagcggg tgtaggccgg ataaggcgcg tcaagcgtcg
    29701 catccggcaa ttgcaccgcg ccactggcgg atgcggcgat taatccagtt gcgtaatatc
    29761 aaaagcggcg cggtcgatca ccgcaatgat cttttttatt tgtgcatcac tgatatcgct
    29821 ctggttgacg cgtaaatcca gcaccgcttt aaaattatcc agcgcccgct tcatttgcgg
    29881 gttctggcgc agcgccgcgc caacgcaacg cgctttgatg cgttcttcaa tcatctccac
    29941 ctgttcgcgg ttttcttcca gccactgcgc gccttgttcg gtcagcgcaa tctgcttctt
    30001 accaccttcc tcttcgcgga tggtaatcag cgactgctcc tgcagaaaat ccagcgtcgg
    30061 gtagatgacg cccgggcttg gggtgtaatt cccctgggtt agattctcaa tcgctttaat
    30121 caattcgtaa ccgtggctgt catcgcgcga gagaatatcc agaatcacca gacgcaattc
    30181 accgtgacca aagaaacgtt gccgacgacc gccgccgcga ccgtggcgac caccgcaaca
    30241 ttgaccgtgt tcatgctggt gaccgtgtcc gcagtgctcg tgttctgact tctcaccttt
    30301 gcagcagccc tcatggcgtg gctggccttc atgtttacaa cacccttcgt gatgatggct
    30361 catatcagcc tccttttgct tgattagata tatctaattt atatctgatg tgagcattac
    30421 tgcaagtaca aaaatgattt ttaaacgtgg aagcacctca catctaggcc ggataagacg
    30481 ttcacgccgc atccggcagc attgctatgg cactgaatgc cttcacatac ttcagacata
    30541 tcagttctac aaatcgcttg catttatcat gattaacaat cattatcatt tgcgaatttt
    30601 atttagatat atctgattac catcacgaag gcgataacaa tgaataacac cccccgctac
    30661 ccgcagcgcg ttcgcaatga tctgcgcttc cgtgaactga ctgtgctacg cgttgagcgc
    30721 atcagcgccg gttttcagcg cattgtcctt ggcggcgagg cgttggacgg ttttacatcg
    30781 cgcggctttg acgatcacag caaactcttc tttcctcaac ctgacgctca ctttgtgccg
    30841 ccaacggtaa cggaagaggg catcgtctgg ccggaaggac cacgcccacc gtcgcgtgac
    30901 tatacgccac tgtatgacga actacgccat gaactggcga tcgatttctt tattcacgac
    30961 ggtggggtcg ccagcggctg ggcgataaac ttacggtggc aggtccgcgc ggttcgctgg
    31021 tggtgccgga agattacgcg tatcagctgt atgtctgcga tgaatccgga atgcccgcac
    31081 tgcgccgccg cctggaaacg ttgagcaaac ttgccgttaa accgcaagtt agtgcgctgg
    31141 ttagcgtgcg ggataacgcc tgtcaggatt atctcgcgca ccttgatggt tttaatatcg
    31201 aatggctggc acatgatgag caggcggtag atgcgcgtct ggcgcagatg caaatccctg
    31261 ccagatgatt acttcatctg gataaccggc gaaggtaaag tcgttaagaa tttaagccgc
    31321 cgctttgaag cggaacagta tgacccacag cgggtacgtg cagcggctta ctggcacgca
    31381 aagtaactga tatataaaaa gaaaaaggct gacgatttct cgtcagcctt tgctatatct
    31441 ggtggcccct gctggacttg aaccagcgac caagcgatta tgagtcgcct gctctaacca
    31501 ctgagctaag gggccatggt agcggattat aaagtaactc cgtgtcgcaa tccagccatt
    31561 accgcgcgcc tgctgttttt ataaacaatg tattttcaat ccgttatact tttcctggtg
    31621 atgttagaaa ggagtaaata tggttgagga tattttggct ccagggttac gggtcgtgtt
    31681 ttgcggtatc aaccctgggc tttcatccgc cgggactggt tttccctttg ctcatccggc
    31741 aaatcgcttc tggaaggtga tatatcaggc cgggtttacc gaccgtcagt tgaagccgca
    31801 ggaggcacag catctgctgg attatcgttg tggcgtcacc aaactggtag accgtccaac
    31861 ggtgcaagcc aatgaagttt caaagcagga gctgcacgca ggtgggcgta agctgattga
    31921 aaaaattgaa gattatcagc cgcaggcgtt ggcgattctg ggcaaacaag catatgaaca
    31981 gggattcagc cagcgcggtg cacagtgggg gaaacaaacg ctcaccattg gttcgacgca
    32041 gatttgggtg ctgccaaatc ccagcggttt aagtcgcgtt tcactggaga aactggttga
    32101 agcgtatcgc gagctggacc aggcgctggt agtgcgtggg cgataaaaaa cgccaccgaa
    32161 aa
//
LOCUS       DAJTXY010000035        31805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTXY010000035 DAJTXY010000000
VERSION     DAJTXY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.035"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(133..1044)
                     /gene="gsiD"
                     /locus_tag="OO566_001720"
     CDS             complement(133..1044)
                     /gene="gsiD"
                     /locus_tag="OO566_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6605033.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1047..1967)
                     /gene="gsiC"
                     /locus_tag="OO566_001721"
     CDS             complement(1047..1967)
                     /gene="gsiC"
                     /locus_tag="OO566_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6605034.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(1985..3523)
                     /gene="gsiB"
                     /locus_tag="OO566_001722"
     CDS             complement(1985..3523)
                     /gene="gsiB"
                     /locus_tag="OO566_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6605035.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(3543..5414)
                     /gene="gsiA"
                     /locus_tag="OO566_001723"
     CDS             complement(3543..5414)
                     /gene="gsiA"
                     /locus_tag="OO566_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6605036.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5401..6366)
                     /gene="iaaA"
                     /locus_tag="OO566_001724"
     CDS             complement(5401..6366)
                     /gene="iaaA"
                     /locus_tag="OO566_001724"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6605037.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6570..7805
                     /gene="moeA"
                     /locus_tag="OO566_001725"
     CDS             6570..7805
                     /gene="moeA"
                     /locus_tag="OO566_001725"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6605038.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPISATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7805..8554
                     /gene="moeB"
                     /locus_tag="OO566_001726"
     CDS             7805..8554
                     /gene="moeB"
                     /locus_tag="OO566_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6605039.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8733..9395)
                     /gene="fsa"
                     /locus_tag="OO566_001727"
     CDS             complement(8733..9395)
                     /gene="fsa"
                     /locus_tag="OO566_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6605040.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9526..10425
                     /locus_tag="OO566_001728"
     CDS             9526..10425
                     /locus_tag="OO566_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6605041.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10431..12863
                     /locus_tag="OO566_001729"
     CDS             10431..12863
                     /locus_tag="OO566_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6605042.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13009..13824
                     /gene="ybiV"
                     /locus_tag="OO566_001730"
     CDS             13009..13824
                     /gene="ybiV"
                     /locus_tag="OO566_001730"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6605043.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13976..15241
                     /locus_tag="OO566_001731"
     CDS             13976..15241
                     /locus_tag="OO566_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6605044.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15482..17074)
                     /locus_tag="OO566_001732"
     CDS             complement(15482..17074)
                     /locus_tag="OO566_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6605045.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPECVIDFSGNYEDYLRSKGIE"
     gene            17293..18213
                     /gene="ldtB"
                     /locus_tag="OO566_001733"
     CDS             17293..18213
                     /gene="ldtB"
                     /locus_tag="OO566_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6605046.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18271..19389)
                     /locus_tag="OO566_001734"
     CDS             complement(18271..19389)
                     /locus_tag="OO566_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6605047.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19386..19853)
                     /gene="mntR"
                     /locus_tag="OO566_001735"
     CDS             complement(19386..19853)
                     /gene="mntR"
                     /locus_tag="OO566_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6605048.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20039..20167
                     /gene="mntS"
                     /locus_tag="OO566_001736"
     CDS             20039..20167
                     /gene="mntS"
                     /locus_tag="OO566_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6605049.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20439..22022
                     /gene="opgE"
                     /locus_tag="OO566_001737"
     CDS             20439..22022
                     /gene="opgE"
                     /locus_tag="OO566_001737"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6605050.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22070..22585)
                     /gene="ompX"
                     /locus_tag="OO566_001738"
     CDS             complement(22070..22585)
                     /gene="ompX"
                     /locus_tag="OO566_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6605051.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22638..22703)
                     /locus_tag="OO566_001739"
     CDS             complement(22638..22703)
                     /locus_tag="OO566_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605052.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            22938..23825
                     /gene="rhtA"
                     /locus_tag="OO566_001740"
     CDS             22938..23825
                     /gene="rhtA"
                     /locus_tag="OO566_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6605053.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24124..24627
                     /gene="dps"
                     /locus_tag="OO566_001741"
                     /gene_synonym="pexB"
     CDS             24124..24627
                     /gene="dps"
                     /locus_tag="OO566_001741"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6605054.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25031..25777
                     /gene="glnH"
                     /locus_tag="OO566_001742"
     CDS             25031..25777
                     /gene="glnH"
                     /locus_tag="OO566_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6605055.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            25916..26575
                     /gene="glnP"
                     /locus_tag="OO566_001743"
     CDS             25916..26575
                     /gene="glnP"
                     /locus_tag="OO566_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6605056.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            26572..27294
                     /gene="glnQ"
                     /locus_tag="OO566_001744"
     CDS             26572..27294
                     /gene="glnQ"
                     /locus_tag="OO566_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6605057.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            27555..29779
                     /gene="ybiO"
                     /locus_tag="OO566_001745"
                     /pseudo
     CDS             27555..29779
                     /gene="ybiO"
                     /locus_tag="OO566_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(29776..30702)
                     /gene="rlmF"
                     /locus_tag="OO566_001746"
     CDS             complement(29776..30702)
                     /gene="rlmF"
                     /locus_tag="OO566_001746"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6605058.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            30978..31238
                     /locus_tag="OO566_001747"
     CDS             30978..31238
                     /locus_tag="OO566_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6605059.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            31503..>31805
                     /locus_tag="OO566_001748"
     CDS             31503..>31805
                     /locus_tag="OO566_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6605060.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT         7731 a         8546 c         7945 g         7583 t
ORIGIN      
        1 aaaaatagtc ccttgctgcg gctttttaac caaattaccc gattctacaa aatcagcaga
       61 aagttacaac acttgcttag ggcaataatt tcctgtgagg tgattaccct ttcaagcaat
      121 attcaaacgt aactatcctt taattttcgg atccagcgca tcgcgtaaac catcgcccaa
      181 caaattgaac gccagtacgg tcagaaaaat agccagggcc ggaaaaacag cgacatgcgg
      241 cgcgataacc atatccgctc gtgcctcatt gagcattgct ccccactctg gtgtcggcgg
      301 ctgcgcacct aggccgagaa atgacaggct ggcggcagag ataatcgagg tgccaatgcg
      361 catggtgaaa aacaccacga tagaagagac ggtcccaggc aggatatgac gcaataaaat
      421 ggtcatatcg ctggcaccaa tactgcgtgc tgactcaata aaggtttgct gtttcaacac
      481 cagcgtgttg ccgcgcacca ggcgggcaaa cgcggggatg gaaaaaatgg cgactgcgat
      541 aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca gcgatcgcca gtaaaatacc
      601 cgggaaggca aacagcacat cgcaaatgcg catgatcagc cgatcccacc agccttcata
      661 atatccagcc aacaagccca gcaacgtccc tatcgccaca ccgataaaca cggcaaacac
      721 gcccgccgcc agcgagattt gcgcaccaac caggacacgg ctgaaaatgt cgcgccccag
      781 tgaatcgacg ccaaaccagt gctgcaaaga aggtccgtta ttcagattgt cataatcaaa
      841 ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa atggccacca caatcaataa
      901 aataacgaat aatgcggcgg tcatcgccat atgctgacgg cgaaatcgtc gccagaattc
      961 atgccacggt gtacgtacct ggtcaggttt gaccagtggc atggcgttta acaccgcctg
     1021 acgtcgccag ttaaatagtc gcatccttac ttgtacctga tagccgggtt aatggcggcg
     1081 taaagcacat ccaccactaa gttgataaga ataaattcca gcgagaaaag cagaatttcc
     1141 gcctgaatca ccgggtaatc gcgcatttct acagagtcaa cgagtaagcg tccaagtccg
     1201 ggccagttga aaactttctc caccacgata gaacctccga gcaaaaagcc aaactgtaag
     1261 cccatcatgg tcactaccgg gatcatcgcg ttgcgtagcc cgtgtttgag gacaacccag
     1321 gtttcgctca cccctttcgc cctcgcggta cgcatataat cttcgcttaa aacgtcgaca
     1381 aatgacgcac gggtaaagcg cgccatcacg gcggccactg ccgcgccgag cgtcagggag
     1441 ggtaaaatgt agtgctgcca actgtctgct cccacagtag gcagccagcc cagttcaacg
     1501 gagaatacct gaattaaaaa catccccagc gcaaatgccg gaaacgagat ccccgacacc
     1561 gcaatggtca tactcaatcg atccggccaa cgattacgcc agacggcggc gataattccc
     1621 gccgccatac caaatatcac cgcccagacc atactggtta tggtcagcca cagcgttggc
     1681 ataaagcggc tggcaatctc atcggcaacc ggacgacgcg acaccatcga caggccaaaa
     1741 tccccctgca cagcattgct gatatagtgc cagaactggt gatacagcgg ctgatccaac
     1801 cccagctgct gacgcaccag ttctataacc tgcgcatcag cttcgggccc ggcaatcaat
     1861 cgcgccggat cgccgggcag catgtggaca aataaaaaca ccagcaccga gacgataaac
     1921 agcgtcggaa tcaaccccag taatcgtttg ataacgtaat taagcattcc actccctgcg
     1981 ttgcttattg caaatccgcg tcttcaaagc tgaagccgct gtctggcatg atccaaaaac
     2041 cggtcaggtt tttactgtgt gccgacacca gtttttctac caccagcggg atccacggcg
     2101 attcttgcca gatgatatcc tgcgccgcct tatataagcg ggtcttttcc gccggatcat
     2161 tcgttttcag tgcctgagcc aggaagtcat ccacctgttt attgctgtaa aacgcggtat
     2221 taaacagcgt cggtggccag ttttgcgagg caaacagcgg cgatagtgcc cagtcggctt
     2281 cgccggttga agccgaccag ccagtgtaga acatccgcac gccgctctct ttttgccctt
     2341 taccttcaac ttctgccgcc cgctgtccgg catccatcgc ggtcacctgg gctttaatcc
     2401 cgacctgcgc taactgctgc tgggtaaatt gcagcacttt ctgcgcggtg ctgtggttat
     2461 gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc cgcctctttc agtaattcgc
     2521 gcgctttcac tggatcgtaa ggccacggtt tataactttg cgcgtaggcg atacttggcg
     2581 gtaccacacc agtagctggc gttgcatagc ccgcaaaggc aactttcacc agcgccggac
     2641 ggttaatggc gtaattcagc gcctcacgga ccttcgggtt atcgaacggc ttttgcgtca
     2701 cgttcatact gatataacgc tgcataattg acggactggc catcaactcg atatttttgt
     2761 ttttctccag cagtgtggcc tgctcgtagg gaatggggaa agcaaactgc gcttcaccgg
     2821 tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg acgccaggtt atgctgtcca
     2881 gtttgggcaa tcctggctgc cagtaacccg cgaatttttt caccttcaca aaatcggtct
     2941 gattccaggt atccagttca tacggtccgg ttcccaccgg atgaaaacca atctccttgc
     3001 catatttttc cagcgctgtc ggtgaaatca tcgcggtcgc cggatgggca agaatattaa
     3061 taaacgctga gaacggctgt ttgagggtaa tctttaccgt tgtcggatcg atcgcctccg
     3121 ttttggcaat attcttatac aggttatagc gtttaagatg attcgccgga tcgctggccc
     3181 ggtccagatt cgcttttacc gccactgcgt tgaaatcggt gccatcctgg aatttaattc
     3241 cttcccgcaa tttcacggtg taagtaatgc cgtcatcgga aacggtataa ctctccgcca
     3301 gcacgttttt cagtttcatc tctttatcca gaccgaacag cccctggtaa aacgatttcg
     3361 ctacggcctg agataacgtg tcattcgcgt catacggatc gagcgtggtg aaattcgatc
     3421 ctaccgccac caccacatct ttggcagcga atgcacaaga tgccatcaac gctgtcgcaa
     3481 tgcccagcgc cactaaccca ctacggtgta cagctcttgc cattttattc tccgcctgaa
     3541 tgttatctac gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg ccccggcccg
     3601 acgcattgca acgagacggc tgccacctct tcgccacgca gatgaatatt gctgggaaga
     3661 tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac cggaactgcc
     3721 gccagtaatt tacgcgtata aggatgctgc gggttttcga agaccgcgca ccgtgaacca
     3781 atttcaacaa tttgcccgag atacatcacc gccacacgat gactaatccg ctctaccaca
     3841 gccatatcgt gggagataaa cagatacgca atgccgaaat cacgctggag atcgagcaac
     3901 aagttgataa tctgcccgcg aatagaaaca tccagcgccg aaacggcttc gtcggcaatg
     3961 atcacttttg gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg ctgaccgccg
     4021 gaaaactcat gcgggtaacg ccaggcatgt tcaggtaaca ggcccacgcg ctccagcaac
     4081 cacgcaacgc gtgcagccgc gtctttacct ggcaataaac cgtgtacacg cagcggttcg
     4141 ataatcgaat caccgatggt ctgacgtggg tccagcgaag cgtaagggtc ctgaaaaata
     4201 aactgaatat cccggcgcaa tgcctgaagt ttgccaggtg acaaggtatc gattcgctga
     4261 ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc gcagcaacgc ccgcccggta
     4321 gtggatttac cgctgccaga ctcgcccacc agcgatagcg tttcgccagg ccagagatca
     4381 aaactgactt tctcaacggc atgcacttcc cgcgttacgc gattcaacaa accgctgcgc
     4441 aaagggaaac gggtgacaag attacgcacc cgtaaaacag gttcgccatc caccaccgtt
     4501 ttctgctcga tggggggttc ctgtttcgct ggatgttcaa gcgatatcaa tgggaaacgt
     4561 cggggataat ctaacccttt catcgcacca agttgcggaa cagcagctaa cagcgcacgg
     4621 gtgtaaggat gttgcggtgc atgaaaaatc tgttcgacgg tacccgtttc caccgcctcg
     4681 ccctgataca tcaccagtac ccgatcggca atctctgcca ccacgcccat atcgtgagtg
     4741 ataaagataa cgcccatcga catctctttt tgcaatactt tgattaattg caggatctgc
     4801 gcctgaatag tgacatccag cgcggtggtt ggctcatcgg caatcagcac cgccgggcgg
     4861 catgacagcg ccatcgcaat catcactcgc tggcgcatcc cgccagagag ttgatgcgaa
     4921 taacgtgaaa gaatggtttg tgcctccgga atgcgtacct gatccagcat ccgcttcgcc
     4981 tcgaccatcg cttcttcacg actggcgttc tgatgcagac gaattgattc ggcaatctgt
     5041 tcacccacag taaatactgg gttcagcgat gtcatcggct cctgaaatat catcgccata
     5101 tccgcaccgc gcacatggcg catttgtgca gcgctctgct cgctaagttc aatcacatca
     5161 cgactgcgcc gccgcaacag cattttatcg cactgtacta aaccgcccgc ctgttccaac
     5221 agacgcatca atgccagcgc agtcactgac ttaccggagc cggattcgcc tacaattgcc
     5281 agcgtctcac cgcgttgcag actaaaagag agattgcgga ccgcagctat tttctgctgg
     5341 tcctgcataa aggcaatatt aagattttca accgccagca cattaccggc atcaagttca
     5401 tcactgtgtg gcaacggtgt cccctttttc acggtagata ccggtggttg gcgtatcgcc
     5461 tgcgtagccc caggcgcgat acattccttc ggtgttaaac ggtagcgcga cattcccttc
     5521 atggtcgata gcgattaagc caccgctacc gccaagcgca gggagttttt ccattactac
     5581 ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc attaacgcgg cgatgtcata
     5641 tgccgccagc gcgcggatga agacttcgcc tgtgccggta caagaaaccg ccacactggc
     5701 gttattggcg tagcatccgg cacccactaa gggactatcg ccaactcgtc cgggtaattt
     5761 attggtcatt ccgcctgtgg acgtggctgc cgccagattg ccgtctaaat ccaacgccac
     5821 ggcccccacg gtgcccattt tctgtttttc atccagtggc gcaccgctat ggtcgaggac
     5881 tgttgcccct tcctcgcgcg ctgccagtag ttgttcataa cgcaaaggcg tggagaaaat
     5941 ctccggtgag acgcgctcca tgccatgagc aaacgcaaaa ttttctgccc cttcgccaat
     6001 catcatcaca tgcgggcttt gctccatcac cagccgggcg gcaagaaccg gattacgcag
     6061 atgactaacg cccgccaccg caccggcttt cagggtgtta ccatccatca cacaggcgtc
     6121 cagttcatgg gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa acagtggaca
     6181 ctcttccagc agacgcaccg cttccgtcac cacatccagc gcactttcgc cggcttccag
     6241 cattttctgc ccggtttcaa caatggcaga caacgcctcg atgtagcgta attcctgttg
     6301 cagactcatc tgcgcgcggc taattgcacc tgcgccacca tgaattgcaa tgactgcttt
     6361 gcccatagta ttcgtccatt aagggtggct cgccactttt gctataaata tcagaatttt
     6421 tatgaattac tcattcaatt tttgagtata gaaagatgga tatggcatgt aaaggcagga
     6481 gccaacgcta cgcccgaggt ggcatacagt ctatggcgtt tttctgacat aataggcaaa
     6541 ttcgattttg cctccgcagg agtgttttca tggaatttac caccggattg atgtcgctcg
     6601 acaccgcgct taatgagatg ctttctcgcg ttaccccact gaccacccag gaaacgctgc
     6661 cactggttca gtgttttggt cgtattctgg cgagcgatgt cgtttcgcca ctggatgtcc
     6721 cggggtttga taactccgct atggacggct acgcggtgcg tttagccgat attgcctccg
     6781 ggcaaccgct gcccgttgcc ggtaaatcct ttgccggtca gccataccat ggtgaatggc
     6841 ctgcgggtac ctgcattcgt attatgaccg gtgcgccggt gccggaaggc tgcgaagcgg
     6901 tggtgatgca ggagcagact gaacaaacgg acaatggcgt gcgttttact gctgaagtgc
     6961 gtagcgggca aaatattcgc cgtcgcggtg aagatatctc tgcaggtgcg gttgttttcc
     7021 cggcgggaac tcgcctgact accgcagagc tgccagtaat tgcttcactg gggattgccg
     7081 aagttccggt gattcgtaaa gtgcgtgtag cgcttttttc taccggtgat gaactccagt
     7141 tgcccggtca gccgctgggc gacggccaaa tctacgacac caaccgtctc gccgtacacc
     7201 tgatgttaga acagctgggt tgcgaggtaa tcaacttagg gattatccgc gacgatccac
     7261 atgccctgcg cgccgcattt attgaagccg acagccaggc ggatgtagtg atcagttccg
     7321 gcggtgtttc agtgggtgaa gcggattaca ccaaaacgat tcttgaagag ctgggggaga
     7381 tcgccttctg gaagctggcg attaaaccag gtaaaccgtt cgcattcggt aaactcagca
     7441 atagctggtt ctgcggcctg ccgggcaacc cgatttcagc gacgctgacc ttctatcaac
     7501 tggtacagcc tttgctggca aaactaagcg gcaacaccgc cagcggcctg cccgcgcgcc
     7561 agcgcgtacg cacagcgtcc cgcctgaaaa aaacgccagg acgtcttgat ttccagcgcg
     7621 gcgtgctgca acgcaacgcc gacggtgaac ttgaagtgac gaccaccgga catcagggtt
     7681 cacatatatt tagctccttt agtctcggca actgctttat cgtgctggaa cgcgatcgcg
     7741 gcaatgtgga cgtgggcgaa tgggtggaag tagaaccgtt taacgcgttg ttcggaggcc
     7801 tgtaatggcg gaactcagcg atcaggagat gctgcgctac aaccggcaaa tcattctgcg
     7861 cggttttgat tttgacggtc aggaggcgct aaaagattct cgtgtgctgg tagtcggcct
     7921 cggtggactc ggctgcgcag cttcgcagta tctggcaagc gccggtgtcg gcaacctgac
     7981 gctgctcgac ttcgacacgg tttcgctctc gaatctgcaa cgccagacac tgcacagtga
     8041 tgccacggtc gggcaaccga aggtggaatc cgcccgtgac gccctgacgc ggatcaaccc
     8101 acatatcgcg attacgccag tcaatgcact gctagatgac gcagaacttg cagcattgat
     8161 tgctgaacac gatctggtgc tcgactgtac ggataacgtt gcggtacgta atcaactgaa
     8221 cgcaggctgt tttgccgcga aggtgccgct ggtttccggc gcggcaattc gtatggaagg
     8281 gcaaatcacg gtcttcactt atcaggacgg cgaaccgtgc tatcgctgcc ttagccgttt
     8341 gtttggcgag aacgcgttaa cctgcgtaga agcaggcgtg atggcaccgt tgatcggcgt
     8401 aattggttcg ttgcaggcaa tggaagcgat caaactgctg gcaggttatg gcaaaccggc
     8461 cagcgggaaa atcgtcatgt acgatgcgat gacctgtcag tttcgcgaaa tgaaactgat
     8521 gcgcaatccg gggtgtgagg tgtgcgggca gtaattgcca cctccaggcc tgataagacg
     8581 cgacaagcgt cgcatcaggc atttgctcca gacgccagat gcggcgtgaa cgccttatct
     8641 ggcctacggt tcgggcacag atttccaagc gcggcaggca tcggtgcact ccgccacgtc
     8701 atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc
     8761 tgccagtcct gctcaaactt cgccacagcg gcttccaccg ccggatagct aatcatctgt
     8821 tgtgccacat ccagtggcag agtaattgat tcacatccgg ccagtaagca gtccagcgcc
     8881 tgacgcgggg ttttgaaact cgctgccagc actttcgcct gcggcgcatg cattttcaat
     8941 aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc aatacgatta
     9001 acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcagcccttg tgccgcgcca
     9061 tataccgcgg ttcccagcgt cggaatccct tccgctttta acatcttaat cgctgccagc
     9121 ccctcggctg tcaccggaac tttcaccacg atatccgcaa taatagaacg cagcttaagc
     9181 gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga
     9241 ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt acccgcggcg
     9301 ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgccttcacc
     9361 gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg
     9421 tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc aatctaactt
     9481 tcattcgaaa gtattttaat gtttgaacga aacagagagg caactatgat tttcaatatt
     9541 cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt
     9601 tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgtacgca ggatctgctg
     9661 tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggccgc gccggaagtg
     9721 attgagcgcg cgctgaatgg tttgcttatt catcgggaaa agttaacccc ggagcatctg
     9781 acggcgttaa ccgactgctg tccgacacag gcgttaaccg tgtgtggtga agtgaaaagc
     9841 gttgaggaga tcatggcgac cgttctgcgc gataaaccgt tttacgatcg cagcggcggc
     9901 ggcttaacgc tttcgggtgg tgaacccttc atgcagccgg aaatggcgac ggcgctgctg
     9961 caagcctgcc acgaggcagg cattcatact gcggtagaaa cctgtctgca tgtgccgtgg
    10021 aaatatatcg ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacgtt
    10081 gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccagggtgct ggataacctg
    10141 aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat tcagggcttt
    10201 aatgccgacg aaacctctgt aaaagccatt accgattttg ccgccgacga gctgcacgtt
    10261 ggcgaaattc attttctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat
    10321 ctgccctatg acgccccgga aaaaccgctt gatgcgccag agctgctcga ttttgcccag
    10381 cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc atgaccacac
    10441 tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg
    10501 tgaaaccgcc agtctgtacc gagcgcgcgc agcactatac cgagatgtat caacaacatc
    10561 tcgataagcc gatcccggta cgtcgcgcgc tggcactggc gcatcacctg gcgaatcgca
    10621 ccatctggat caaacactat gagttgatca ttggcaacca ggcaagcgaa gttcgcgccg
    10681 cgccgatctt cccggaatat actgtctcgt ggatcgaaaa agagattgat gatctggcag
    10741 atcgtcccgg tgctggcttt gcggtgagcg aagagaacaa acgtgttctg catgaagtgt
    10801 gcccgtggtg gcgcggtcag acggtacagg atcgctgcta tggcatgttt accgatgagc
    10861 aaaaaggtct gctggcgacc ggcatcatta aagcggaagg caatatgacc tccggcgatg
    10921 cgcacctggc ggtcaatttc ccgctgctgc tggaaaaagg gcttgatggt ctgcgtgaga
    10981 aagtagcgga acgtcgctcg cgcatcaatc tgacggtgct ggaagatttg cacggcgaac
    11041 agttcctgaa agcgattgat atcgtgctgg tggcagtcag tgaacacatt gaacgtttcg
    11101 ctgccctggc gcgtgcaatg gccgcgaccg aaacccgcga aagccgtcgc gatgaactgc
    11161 tggcgatggc agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg
    11221 cgctgcaact gtgttacttc atccagttga ttttgcagat tgaatctaac ggtcactcgg
    11281 tgtcgtttgg tcgtatggat cagtatctct acccgtacta tcgccgcgac gttgaactga
    11341 accagacgct ggatcgcgaa cacgccatcg aattgctgca tagctgctgg ttgaaattgc
    11401 tggaagtgaa caaaatccgc tccggctccc actcaaaagc ctctgcggga agtccgctgt
    11461 atcagaacgt caccattggc ggacaaaatc tggttgatgg tcaaccaatg gacgcggtga
    11521 atccactctc ttacgcgatc ctcgaatcct gcggtcgcct acgttccact cagcctaacc
    11581 tcagcgtgcg ttaccatgca ggaatgagta acgatttcct cgacgcctgc gtacaggtga
    11641 tccgctgcgg cttcgggatg ccggcattca acaacgacga aatcgtgatc ccggaattta
    11701 ttaaactcgg tattgaaccg caggacgctt atgactacgc agcgattggt tgtatagaaa
    11761 ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc
    11821 gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac cagcggcaaa gtgttcctgc
    11881 cacaagaaaa agcgttgtcg gcaggtaact tcaacaactt cgatgaagtg atggccgcgt
    11941 gggatacgca aatccgttac tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca
    12001 ccatgctgga agagaacgtg cacgatattc tctgctcggc gctggtggat gactgtattg
    12061 agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg
    12121 tcggtattgc caacctcggc aacagcctgg cggcagtgaa gaaactggtg tttgaacagg
    12181 gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat ggcctgactc
    12241 atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata
    12301 ccgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg aaacagtacc
    12361 ataacccacg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt acgtcgtcta
    12421 tctccgctaa cgtaccgttt ggcgcgcaaa ctatggcaac gccggacggg cgtaaagccc
    12481 acaccccgct ggcagaaggc gctagtccgg cctctggtac tgaccatctc ggccctactg
    12541 cggtcattgg ctcagtgggt aaactgccta cggcagcgat tctcggcggc gtgttgctca
    12601 accagaaact gaatccggca acgctggaga acgaatctga caagcagaaa ctgatgatcc
    12661 tgctgcgtac cttctttgaa gtgcataaag gctggcatat tcagtacaac atcgtttccc
    12721 gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg gtagtgcgtg
    12781 tcgcgggtta ttccgccttc ttcaccgcgc tctctccaga cgctcaggac gatatcatcg
    12841 cccgtactga acatatgctg taatctcttc ggcccggctg cggcgtcgcc gggctaaatc
    12901 attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc tctgcgtcac
    12961 attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa
    13021 gttatcgtca cagatatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca
    13081 cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttaagttcgt tgtcgccagc
    13141 ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga gatctctttt
    13201 gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca cggcgaactg
    13261 acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt
    13321 gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca
    13381 ctgatggcaa aacactacca tcgcctgaaa cctgtaaaag attatcagga gattgacgac
    13441 gtactgttca agttttcgct caacctgccg gatgaacaaa tcccgttagt gatcgacaaa
    13501 ctacacatag cgctggatgg cattatgaaa cccgtcacca gtggttttgg ctttatcgac
    13561 ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat
    13621 ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa
    13681 atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt
    13741 tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc tgtgctggat
    13801 aacacatccc cttttaacag ctgacctctc taccggagcc atgcgctccg gttttccctt
    13861 ctttttacat cctgtacatc acatcaaatc attaaacttg cattaactta tttataactt
    13921 gtattgtcac atgcaaatca gcatagtttc gaatgaaagt taatgcgagg tggttatggc
    13981 ttctactttt accagcgaca cattgcctgc cgatcacaaa gcagctatcc gtcagatgaa
    14041 gcacgcgctg cgggcgcagc ttggcgatgt ccagcagatc tttaatcagc taagcgatga
    14101 cattgccacg cgagtggctg aaatcaacgc actcaaagca cagggcgatg ccgtctggcc
    14161 ggtgctgtct tatgccgata tcaaagcagg tcatgttact gcagagcagc gcgaacagat
    14221 taaacgtcgc ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag cgctaggctg
    14281 ggatcagtcg atgctggact atctgaaccg caaccgcttt gacgaggtct acaaaggccc
    14341 cggcgataat ttcttcggga cgctcagcgc ttcacgtccc gagatttacc ccatctactg
    14401 gtcgcaggcg caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct
    14461 caatcgtctg tggacatttg aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt
    14521 catctaccct gaccgtatcc gccgccttcc gcccggaacg acctccaaag gtcttggagc
    14581 gcataccgac tccggggcgc tggaacgctg gctgcttcca gcgtatcagc acgttttcgc
    14641 caacgtcttt aatggcaatc tggcgaaata cgatccctgg catgcggcac atcgtacgga
    14701 agttgaagag tacacggtgg acaacaccac caaatgttcc gtgtttcgga cattccaggg
    14761 ctggactgcg ctctcggata tgctgcctgg tcaggggttg ctgcacgtcg tgcccattcc
    14821 tgaagccatg gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg aggatgaact
    14881 gtgcggcgta gcgcccggaa gagtgttgcc ggtatcagag caatggcatc cactgttaat
    14941 tgaggcgtta accagcattc caaaactcga ggccggagac tccgtctggt ggcactgcga
    15001 cgtcatccat tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg tgatgtacat
    15061 tcctgcggca ccgatgtgcg agaaaaatct tgcctacgcg cacaaggtga aggccgcact
    15121 ggaaaaaggc gcatcgccgg gcgacttccc gcgcgaggac tatgaaacaa actgggaagg
    15181 acgctttacg cttgccgacc tcaacattca cggtaagcga gcgttgggca tggatgtttg
    15241 agagttaatg gaagtgaatt gatgtgggat taagaaaatc gattcgacgt tactcagatt
    15301 gctgacaagt gcgctttctt aatgcctgat gcggcgttaa cgccttatcc ggcctacaaa
    15361 gttctgcaga ttcaatatat tacaggtact gcgtaggcct gataagcgta gcgcatcagg
    15421 ctattttgca tttgtcatca gccgtcgtca ccccgacagt atcggggtga cggataatca
    15481 cttactcgat ccctttacta cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc
    15541 attccggggt gatttccaga atgcgggtcg ccagggagct tacgaactca cggtcgtgtg
    15601 aaacaaagat cagcgtgccc tgatacagtt ccagcgccat gttcagtgac tcgatggatt
    15661 ccatatccag gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta
    15721 acttaccaaa cagcatccgc cctttttccc caccggaaag cactttagct ggctttttga
    15781 tgtcgtcctg gctgaacagc aaacgaccga gaatgctgcg taccgcctgc tcgtcatcgc
    15841 cttcctgctt ccactgactc atccattcga acacggtcag atcattttca aactcatatt
    15901 cgtggtcctg ggcgtagtaa ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt
    15961 ccggttgcag atcgcccacc agcgttttca gcagcgttga tttaccgacg ccgttggtac
    16021 ccagtaccgc aagtttttca ccgacctcca gcagcaggtt gagattttta aacagtggac
    16081 cgttatcaaa ccctttggtc agaccttcca cttccagcgc gttacggaac agtttcttat
    16141 cctgttcaaa acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga
    16201 ttttatcaat ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa
    16261 agcggctaac gaaagattgc aactcagcaa tctgcgcttt cttcttggcg ttatcggcca
    16321 gcagacgttc acgcgcctgg gtcgccgccg tcatgtactc atcgtagtta cccggataaa
    16381 cgcgcagctc gccgtaatcc agatccgcca tgtgggtaca gaccatgtta aggaagtgac
    16441 ggtcgtgcga gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc
    16501 gaatggtgtc gatgtcgagg ttgttggtcg gttcgtcgag caggagaata tccggctcag
    16561 caaacagcgc ctgcgccaga agcacacgca gcttccagcc aggagcaact tcactcatcg
    16621 ggccgtagtg ctgttccact ggaattccca cgccaagcaa cagttcaccg gcgcgagctt
    16681 ccgcagagta accgtccatt tcgccgtatt tcacttccag atcggccact ttatagccgt
    16741 cttcttcact catttccggc aaagcataga tgcggtcgcg ctcctgcttc acttcccaca
    16801 actctttatg ccccatgatc accgtatcca gcacagtgaa cccttcaaag gcaaactgat
    16861 cctgacgcag cttaccaatg cgctcgttgg gatcgaggga aacgttaccc agcgtcggct
    16921 caaggtcgcc gccgagaatc ttcataaagg tggatttacc actaccgttc gcgccaatca
    16981 ggccgtaacg gttgccgccg ccgaatttga cggaaatgtt ttcaaacaac ggcttactgc
    17041 cgaactgcat ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac
    17101 aaaccgcgca ttatgccata agtctgaaag aagatcctgc tcctcgctta aggtctacac
    17161 tcaagccaaa ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg
    17221 aacttataat gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa
    17281 atctcgctta acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc
    17341 ggcttttgca gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg
    17401 gttggtcaga atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt
    17461 gccgcggagt accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc
    17521 ttcctgccga aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc
    17581 gttcatgaag gcatcgtcat taacagtgca gagatgcgtc tgtattacta tccgaaaggg
    17641 accaacaccg ttatcgtgct gccgatcggc attggtcagt taggcaaaga tacgcctatc
    17701 aactggacca ccaaagttga gcataagaaa gcaggcccga cctggacgcc gaccgccaaa
    17761 atgcacgcag agtaccgcgc tgcgggcgaa ccgctaccgg ctgtcgttcc ggcaggtccg
    17821 gataacccga tgggtctgta tgcactctac atcggtcgcc tgtatgctat ccatggcacc
    17881 aacgccaact tcggtatcgg cctgcgtgta agtcatggtt gtgtgcgtct acgtaacgaa
    17941 gacatcaaat tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag
    18001 ccggtaaaag cgaccaccga gccagacggc agccgttata ttgaagtcca taacccgctg
    18061 tctaccaccg aagcccagtt tgaaggtcag gaaatcgtgc caattaccct gacgaagagc
    18121 gtgcagacag tgaccggtca gccagatgtt gaccaggttg ttcttgatga agcgatcaaa
    18181 aatcgttccg ggatgccggt tcgcctgaat taatcgtcaa aacgtaaaac aaaggcggac
    18241 tcataatccg ccttttttat ttgccagacc ttaccaggcc gggatcataa ctaacaaaac
    18301 gtatcccacc agcgccgccc acaacagcat cggtattgaa tagagatgga aacgccacca
    18361 gatgcggcga tcgttcgcca tacgtagcgc aatcaaattt gccagcgatc ctggtaataa
    18421 accaaagccc cccacgttta ccgcccatgc cagtaataaa gacggaggta cataattgag
    18481 cagcaaaatg gtactcggca cgttactgat cacctgcgat aaaccgactg ccgttaacca
    18541 taacccgggt tcagataaat gactcacgtt acccaacacg ccttgcaacg ctggaagctg
    18601 ggtcagtaaa tggacgtcga taaacatcgc cataaacacc agcagcaacg tccagtccac
    18661 actcaacacc acgcgacgcg ccagcagcac aaagcccgcc gccacaatca ccagtcccca
    18721 cagctcttgt ttgaactcca gcgccgtcag aaaaacgata tacagcccca gacaattcca
    18781 caccagccgc ggtttccact ccggtgtttg catccccgta tggtattgca gcgcctttcc
    18841 agggaaacaa caccagcaca acagcaggag tgtcagcatc attgcgccag ccagcggtgc
    18901 catttgggca ataaatccgg caaacgaaag accagaacgt ccccagataa gaatattttg
    18961 cgggttgcca attggcgtca gtagcgaacc agcgttgact gccagcgcct caaaaataat
    19021 cagccgatta accgggatct cacacagtct ttttagcgtg atagtcagcg gaacaacaat
    19081 aaacagcgcg acatcgttgg tcagaaaggt agaaagcagc gccgccgcca gcaccataaa
    19141 catcgccagc cgacgctccg tagcaaagcg gcgcaccatt ttgcgcccca gcacatcaaa
    19201 ataaccgctt aactccacac ctttggtcag cagcatcagg ccgcttaagg tgatgatggt
    19261 gtgccagtcg atagcagcag gccaggattt cggtgcaaag ggcacgaaaa agcttaatcc
    19321 gataccaaca agaattaata actgaaaaaa acgatcgcct tgcagcgtgc gtaaaaaagg
    19381 caggctcatt tggcaccgtg tttctgggta aacaaacgaa aagcgtcgag cgtctcttca
    19441 ctaacatggt gctccatgcc ttccgcgtcg cgacgggcga tttccggact gacgcccaac
    19501 accaacaaga aattttcgac tatctgatgt cgctcgcggc tttcctgcgc cagcttctct
    19561 ccttctgccg ttaaaaacac gccacgccag gggatcattt caatcagccc catggttgcc
    19621 agccgcttaa gcattttagc caccgtcggt tgcgaaactc ccagacgagc agccatgtcc
    19681 acctgacgag cttcccccac ttccctgatc aagtcagaaa tcagctcaac gtaatcatca
    19741 ataagctcgc gccgatgcgc ctctctgacc tggcggaacc cttcaacgtg ctcttccacg
    19801 ttcactaact gcgtcacttt ttttgctgtt ggcgtacctg cgcgacgact cattgtgctt
    19861 cctcattatg gtgacgcata catagcgttc tcatttcgtg cgcacattgt aaaccagagt
    19921 tgcgaaggta caaaaaatta acgttttagc aatagctata taatatagcc tgtgctatat
    19981 ctgtatgtaa tgcaatcatc cctcaaggat cgacgggatt agcaagtcag gaggtcttat
    20041 gaatgagttc aagaggtgta tgcgcgtgtt tagtcattct ccctttaaag tacggttaat
    20101 gctgctctct atgttgtgcg atatggtcaa caacaaaccg cagcaagata aaccttcaga
    20161 taaatagcgg cgtcgcggta cgccgcttca ctcctgcttt tatgcaggca taacgcgttt
    20221 tggtctgaaa aaaaccactt tttgtcggat ttgcaatccc cttcgcaaaa gatttgttcg
    20281 tcagtagttg acctgaacgg cggctcgctc tatcttcttg cagccctgcg tatattgcgg
    20341 ctcgcggatg cggacccctt tcccctcttc acgcactctt gcaggtattg acccttgacg
    20401 ccagggtaag cacatggcgt ttgttaccat agtggcatat gaatttaacc ctcaaagaat
    20461 cgcttgttac ccgtagccgg gtatttagcc cgtggactgc gttctacttt ttacagtcgc
    20521 tattaattaa cctcggctta ggttacccct tcagtttgct ctacaccgct gcgtttacgg
    20581 ctattttgct tttgctatgg cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca
    20641 gttcgctggt ggcggcttgt tatttccctt ttgctcaggc ctacggcgcg cctaatttca
    20701 atacattgct ggcattgcac tccaccaata tggaagagtc gaccgaaatc ctgacgattt
    20761 ttccgtggta cagctacctg gtcggcttat ttatttttgc gctcggcgta atagcaatca
    20821 ggcgaaaaaa agagaatgaa aaagcgcgct ggaatacctt cgacagcctg tgtctggtat
    20881 tcagtgtggc gacatttttt gttgctcccg tgcaaaacct ggcctggggt ggcgtattta
    20941 aactgaaaga tactggctat ccggtatttc gttttgctaa ggatgtcatc gtcaataata
    21001 acgaggtgat tgaagagcaa gaacggatgg caaaactttc cggaatgaaa gatacctgga
    21061 cggtcactgc cgttaagccg aagtatcaga cctatgtggt agtgatcggt gaaagcgcgc
    21121 gtcgcgatgc cctcggtgcc tttggcggtc actgggacaa taccccgttt gccagcagcg
    21181 ttaacggttt gatatttgct gactacattg ccgccagtgg ctccacgcag aaatcgcttg
    21241 gcttaacgct caatcgcgta gtcgatggca aaccacagtt tcaggataac tttgtcaccc
    21301 tggcaaatcg cgcgggcttc cagacctggt ggttttccaa ccagggccaa atcggcgaat
    21361 acgatactgc tatcgccagc atcgccaaac gtgcagatga agtgtacttc ctgaaagaag
    21421 gtaattttga agcagataaa aacacgaaag acgaagcgtt actggatatg accgctcaag
    21481 tgctggcgca agagcactcg caaccgcagc tgattgttct gcatctgatg ggctcgcatc
    21541 cgcaggcctg cgacaggaca caaggcaaat acgaaacctt tgtgcaatcg aaagaaacgt
    21601 cgtgctatct ctataccatg acgcaaacgg acgatttact gcgcaagctg tacgatcagt
    21661 tacgcaacag cggcagcagc ttctctctgg tttacttttc tgaccacggt ctggccttta
    21721 aagagcgcgg caaagacgtg caataccttg cccatgatga taaatatcag caaaatttcc
    21781 aggtgccttt tatggtcatt tccagcggcg ataaagcgca tcgggtgatt aaagcccgcc
    21841 gctcagccaa tgacttctta ggctttttct cgcagtggac gggaattaaa gcgaaggaaa
    21901 taaatatcaa atacccgttt atatctgaga agaaagccgg gccgatatac atcaccaact
    21961 tccagttaca gaaggtggat tacaaccatc tcggaaccga tattttcgac ccgaaacctt
    22021 aaaaacaaaa atccgccccg agaggcggat ttttatatca ccaaagtgat tagaagcggt
    22081 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
    22141 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
    22201 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
    22261 ccacacccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
    22321 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
    22381 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
    22441 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
    22501 ttacggtaga agtcgcagct acggaagtgc ctgcggtgaa agccagaact gcggccagtg
    22561 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
    22621 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
    22681 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
    22741 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
    22801 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
    22861 acaaatgctc aaccagcatt gggtatatcc agtacactcc acgctttact taagtctaga
    22921 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaatag
    22981 tcatattact cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
    23041 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
    23101 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
    23161 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
    23221 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
    23281 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
    23341 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
    23401 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
    23461 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
    23521 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
    23581 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
    23641 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
    23701 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
    23761 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
    23821 attaaattta catttctgta tggttatgca taaccatgca gaatttctcg ctacttttcc
    23881 tctacaccgt ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc
    23941 gagcagataa ataagaattg ttcttatcaa tatatctaac tcattgaatc tttattagtt
    24001 ttgtttttca cgcttgttac cactattagt gtgataggaa cagccagaat agcggaatac
    24061 atagccggtg ctatacttag tctcgttaat tactgggaca taacatcaag aggatatgaa
    24121 attatgagta ccgctaaatt agttaaatca aaagcgacca atctgcttta tacccgcaac
    24181 gatgtctccg acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca ggttatccag
    24241 tttattgatc tttctttgat taccaaacaa gcgcactgga acatgcgcgg cgctaacttc
    24301 attgccgtac atgaaatgct ggatggcttc cgcaccgcac tgatcgatca tctggatacc
    24361 atggcagaac gtgccgtgca gctgggcggt gtagctctgg ggaccactca agttatcaac
    24421 agcaaaaccc cgctgaaaag ttacccgctg gacatccaca acgttcagga tcacctgaaa
    24481 gaactggctg accgttacgc aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg
    24541 aaagatgacg acaccgcaga tatcctgacc gccgcgtctc gcgacctgga taaattcctg
    24601 tggtttatcg agtctaacat cgaataaatc catcgctgat ggtgcagaac tttagtaccc
    24661 gataaaagcg gcttcctgac aggaggccgt tttgttttgc agcccacctc aacgcactta
    24721 tttagtgcat ccatctgcta tccccagctg attaagtaaa ttttttgtat ccacatcatc
    24781 acacaaccgt tacataaaga ttgttttttc atcaggtttt acgctaaata atcactgtgt
    24841 tgagtgcaca attttagcgc accagattgg tgccccagaa tggtgcatct tcagggtatt
    24901 gctctataaa tcgtgcatca cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaac
    24961 tggcacgatt ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa
    25021 aggaaatgct atgaagtctg tattaaaagt ttcactggct gcactgaccc tggcttttgc
    25081 ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg acggataccg ccttcgttcc
    25141 gtttgaattt aaacagggcg ataaatatgt gggctttgac gttgatctgt gggctgccat
    25201 cgctaaagag ctgaagctgg attacgaact gaagccgatg gatttcagtg ggatcattcc
    25261 ggcactgcaa accaaaaacg tcgatctggc gctggcgggc attaccatca ccgacgagcg
    25321 taaaaaagcg atcgatttct ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa
    25381 agctaacaat aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa
    25441 gagcggtact ggctccgttg attacgcgaa agcaaacatc aaaactaaag atctgcgtca
    25501 gttcccgaac atcgataacg cctatatgga actgggcacc aaccgcgcag acgccgttct
    25561 gcacgatacg ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc
    25621 ggtaggtgac tctctggaag cgcagcaata cggtattgcg ttcccgaaag gtagcgacga
    25681 gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga
    25741 agtctacaaa aaatggttca gtactgaacc gaaataataa cgctacacct gtaaagcgca
    25801 ctggcagttc cctctccccc ctggggagag gattagggtg aggggcgcaa acccgctccg
    25861 gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac aacatatgca
    25921 gtttgactgg agtgccatct ggcctgccat tccgcttctg attgaaggtg ccaaaatgac
    25981 cctgtggatt tcggtcctcg gtctggctgg cggtctggta atcggattgc tggcaggttt
    26041 tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat
    26101 ccgcggcaca cctatcgtcg tccaggtgat gtttatctat ttcgccctgc cgatggcgtt
    26161 taacgactta cgcatcgacc catttactgc ggcggtggtc accatcatga tcaactccgg
    26221 cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga
    26281 agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca ttttaccgct
    26341 ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca ttaaagacac
    26401 ctcactgttt attgttatcg gcgtggcgga actgacccgt caggggcaag aaattattgc
    26461 cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgttctatc tgattattac
    26521 cctggtgctg agctttattc tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa
    26581 tttaaaaacg tctccaagca ctttggccca acccaggtgc tgcacaatat cgatttgaac
    26641 attgcccagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa atcgaccctg
    26701 ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt cgatggcctg
    26761 aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc
    26821 cagcagtttt acctcttccc gcatctgacg gcgctgggaa acgtcatgtt tggtccgcta
    26881 cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa
    26941 gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg tcaacagcag
    27001 cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg
    27061 acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca ggatctggct
    27121 gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga gaaagtagct
    27181 tcgcggctga tctttatcga caaaggccgg attgcggaag atggcaatcc gcaggtgttg
    27241 atcaagaacc cgccgagcca gcgcttgcag gaatttttgc agcacgtctc ttaataagac
    27301 acattgcctg atgcgctacg cttatcaggc ctacaggata tctggcaact tattaaaatt
    27361 gcatgaactt gtaggacgga taaggcgttc acgccgcatc cggcaaaaaa gcccgcacgt
    27421 tgtcagcaac ctgcttaata tcccttcctc cctttcaccc gaaagggagg cacaccagat
    27481 tcctctcatt taaaatcgcc cctcccccag catctatact tatctttttg ctctgttttc
    27541 tcactggagg agtcatgcgg tggatcctgt tcatcctctt ctgcctgctg ggcgcacctg
    27601 cccacgcggt atccataccc ggcgttacaa ccacaacgac aacggactca acgactgaac
    27661 cggccccgga accggatatc gaacaaaaaa agcggcctat ggcgcactgg cggatgtgct
    27721 ggataacgac acctcgcgta aagagttgat cgaccagttg cgtactgtcg ccgcgacgcc
    27781 ccctgcggaa ccggtcccga agatcgtgcc gccgacgctg gttgaagagc aaaccgtgct
    27841 gcaaaaggtc accgaagtca gccgccatta tggtgaagcc ctttccgccc gcttcgggca
    27901 actttatcgc aatatcaccg gctccccgca taagccattt aatccacaaa ccttcagcaa
    27961 tgcgctgagc catttttcaa tgttagcggt attagtgttt ggtttttact ggctgattcg
    28021 cctgtgcgca ctgccgctat atcgcaaaat gggtcagtgg gcgaggcaaa aaaatcgtga
    28081 gcgcagcaac tggttgcagc ttccggcgat gattattggg gcgtttatta tcgacctgct
    28141 gttactggcc ctgacattat ttgtcggcca ggtattaagc gacaacctga atgcaggtag
    28201 tcgcaccatc gctttccaac aaagtttgtt tctcaacgcc tttgccctga ttgaattttt
    28261 caaagccgta ctacgcctga ttttttgccc aaacgtggcg gagctgcgcc cgttcacgat
    28321 tcaggacgag agcgcccgtt actggagtcg ccgcctgagc tggttaagca gcctgattgg
    28381 ctatggcctg attgtggccg tgccgattat ctctaatcag gtgaatgtac agataggtgc
    28441 gctggcgaac gtcatcatta tgctgtgcat gaccgtctgg gcgttgtacc tgatcttccg
    28501 aaataaaaaa gagattaccc agcatttgct caactttgcg gagcattcgc tggctttttt
    28561 cagcctgttt atccgcgcct ttgcgctggt gtggcactgg ctggcaagcg cctattttat
    28621 cgtgctgttt ttcttttcgt tgttcgatcc gggcaacagt ctgaaattta tgatgggtgc
    28681 aacggtgcgc agcctggcga ttattggtat cgcagcgttt gtttccggta tgttttcccg
    28741 ctggctggcg aaaaccatca ccctctcgcc acatactcag cgtaactatc cggagctaca
    28801 aaaacggctg aatggctggc tttcggcggc gctgaaaacg gcgcgtattc tgaccgtctg
    28861 cgtggcagta atgttgctgt tgagcgcatg gggattgttc gatttctgga actggctgca
    28921 aaacggcgcg gggcagaaaa ccgtagatat cctgatccgc atcgcactca ttcttttctt
    28981 ctcggcggtt ggctggacgg tgctcgccag tttgatcgaa aaccggctgg cttcggatat
    29041 tcatggccgc ccgctaccca gcgcccgtac gcgtaccctg ctgacgctgt tccgtaacgc
    29101 gctggcggtg attatcagta ccatcaccat catgattgtg ttgtcggaaa tcggcgtcaa
    29161 tatcgcgcca ttgctggcag gtgccggggc attaggtctg gctatctcgt ttggttcgca
    29221 aacgctggta aaagatatta tcaccggggt atttattcag tttgaaaacg gcatgaacac
    29281 tggagatttg gtgactatcg ggccgttgac cggcacagtg gaacggatgt cgattcgctc
    29341 cgtgggcgtg cgccaggata ccggggcgta tcacatcatt ccgtggtctt cgataaccac
    29401 ctttgctaac ttcgtccgcg gtattggttc ggtagtggca aactacgatg ttgatcgcca
    29461 tgaagatgct gataaagcca atcaggcact gaaagatgcg gtaacggaat tgatggaaaa
    29521 cgaagaaatt cgcgggctga ttattggtga accgaacttt gccgggattg tcggcttaag
    29581 caataccgcg tttacactgc gtgtttcgtt caccacgctg ccactcaaac agtggacggt
    29641 acgctttgcc ctcgacagcc aggtgaaaaa acatttcgac ctggcgggcg ttcgcgcgcc
    29701 agtgcagact tatcaggtgc tgcctgctcc gggcgcgacc ccggctgaac cgctgccgcc
    29761 gggggagcca acgctttaac gttggcgatt aacaaaacgg cggcgctgct cgtcgttcat
    29821 aaaggtccag gcaataaagc gactctgctt ttgcccctgg gccatctctt tttttaccac
    29881 cttcaccgcg cccacgtcgg tcagagcacg atacaacggc ggtaagtttt caccacgaga
    29941 taccagcgag gtaaaccaca tcacctgctt cgcgaagcct ttgctctctt caatcatctt
    30001 tttgataaag ttgacttcac cgccttcaca ccacaactcc tgttgctgac caccaaagtt
    30061 cagtgcatcg tctttgttca gccccaggtt acggcgttta cgctcactac ctgcccgcgc
    30121 cgcagcggcg gaatcgtgga atggcgggtt acacaaggtc gcgtcgtatt gctcgttttt
    30181 gtggatgatg ccgttaaaaa tcgccccact ctctttttgc cgacgcagac gaatggcgcg
    30241 gttaagcccc ggattagcac tgataatcgc ctgcgcactg cttaacgcct ggctgctggt
    30301 ttcactaccg gtaaaacgcc agccatattc atgtacgcca atcagcggat aaatacagtt
    30361 cgcaccaacg ccgatatcca gaatgctggc attcgctgga attgttccgc ttgcttcagc
    30421 cagtaaatcg gcaaggtgat gaatataatc cgcccggccc ggtactggtg ggcagagaaa
    30481 accgtcgggg atatcccagt tcgctacggc gtaaaaatgg gccagcaacg ccttattgag
    30541 cgccttcacc gccagcggat tggcaaagtc tacgctttgc tcccctgcgg gtgtaagcgt
    30601 gaggaattgc ctgagttcag gattgacctg acaaagcgtg gcgagatcgt agcggctgtg
    30661 atgacggttg cgcggatgca accccggttt ctgggcggac atagcattct cctttaagca
    30721 gcggcgtaag atacccgcat aacgcgacag ggtaaatatc tggctgcgtt tatcattccc
    30781 caaaacctta acatggctat ggtttatggc aattcatcat aaaaatcagc attatgcatg
    30841 tcattgattt gtattatttt gtaaaaatat atcgttcaaa aaataagcat atctctgtca
    30901 attttttgcg tcatttgcat cgcgcaggcg acctacactt atcaggcact acccgttgta
    30961 tttatgagga tgtcattatg aaaaagtgcc tcacactact gattgccacc gtcctgagcg
    31021 gaatctctct cacggcttat gccgcgcaac cgatgagtaa cctggacagc ggtcagctac
    31081 ggcccgccgg taccgtttcg gcaactggcg catcaaacct aagcgatctg gaggataaac
    31141 tggcagaaaa agcgcgcgaa caaggcgcga aaggttatgt cattaattcc gcaggcggaa
    31201 atgaccagat gttcggtact gcaaccatct acaaataacc ccaccaacgt aactgcattc
    31261 gcctctggat gcagttttcg cctgttttgt aatcaaaaaa catcaaatat gaacgcaatg
    31321 taaataaatg tatttctttt tcgcgcaatg ggtgatagaa aatcgcgtca agtgataatg
    31381 cttatcaaaa ttattatcac ttgcacaaac actatcacgg gattaacagt ggcatcgcat
    31441 ccgcagagat gctttctcgt ggcagtgaaa atttcaacat ataagaaaaa gtcacctgca
    31501 aaatggaaaa caatcgcaat ttccctgcca gacaatttca ttcgctcacg ttctttgccg
    31561 gtctttgtat tggtattacg cctgtggcac aggcactcgc cgccgaaggg caagctaacg
    31621 cggatgacac gctggttgtc gaagcatcaa cgccttcgct ttatgcgcca caacaatctg
    31681 ccgatccgaa attctcgcgt ccggtagcgg atactacccg cacgatgacg gtgatttctg
    31741 aacaagtgat taaagatcag ggcgcaacca accttaccga cgcgctcaaa aacgtccccg
    31801 gcgtg
//
LOCUS       DAJTXY010000036        30667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTXY010000036 DAJTXY010000000
VERSION     DAJTXY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.036"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..521
                     /gene="tuf"
                     /locus_tag="OO566_001749"
     CDS             <1..521
                     /gene="tuf"
                     /locus_tag="OO566_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6605061.1"
                     /translation="GRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLL
                     DEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFF
                     KGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGR
                     TVGAGVVAKVLS"
     gene            751..1134
                     /gene="secE"
                     /locus_tag="OO566_001750"
     CDS             751..1134
                     /gene="secE"
                     /locus_tag="OO566_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6605062.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1136..1681
                     /gene="nusG"
                     /locus_tag="OO566_001751"
     CDS             1136..1681
                     /gene="nusG"
                     /locus_tag="OO566_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6605063.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1840..2268
                     /gene="rplK"
                     /locus_tag="OO566_001752"
     CDS             1840..2268
                     /gene="rplK"
                     /locus_tag="OO566_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6605064.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2272..2976
                     /gene="rplA"
                     /locus_tag="OO566_001753"
     CDS             2272..2976
                     /gene="rplA"
                     /locus_tag="OO566_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6605065.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3389..3886
                     /gene="rplJ"
                     /locus_tag="OO566_001754"
     CDS             3389..3886
                     /gene="rplJ"
                     /locus_tag="OO566_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6605066.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3953..4318
                     /gene="rplL"
                     /locus_tag="OO566_001755"
     CDS             3953..4318
                     /gene="rplL"
                     /locus_tag="OO566_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6605067.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4638..8666
                     /gene="rpoB"
                     /locus_tag="OO566_001756"
     CDS             4638..8666
                     /gene="rpoB"
                     /locus_tag="OO566_001756"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6605068.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGELVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8743..12966
                     /gene="rpoC"
                     /locus_tag="OO566_001757"
     CDS             8743..12966
                     /gene="rpoC"
                     /locus_tag="OO566_001757"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6605069.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13208..14341)
                     /gene="thiH"
                     /locus_tag="OO566_001758"
     CDS             complement(13208..14341)
                     /gene="thiH"
                     /locus_tag="OO566_001758"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6605070.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14338..15108)
                     /gene="thiG"
                     /locus_tag="OO566_001759"
     CDS             complement(14338..15108)
                     /gene="thiG"
                     /locus_tag="OO566_001759"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6605071.1"
                     /translation="MLRIVDKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15110..15310)
                     /gene="thiS"
                     /locus_tag="OO566_001760"
     CDS             complement(15110..15310)
                     /gene="thiS"
                     /locus_tag="OO566_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6605072.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15294..16049)
                     /gene="thiF"
                     /locus_tag="OO566_001761"
     CDS             complement(15294..16049)
                     /gene="thiF"
                     /locus_tag="OO566_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6605073.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTADINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16042..16677)
                     /gene="thiE"
                     /locus_tag="OO566_001762"
     CDS             complement(16042..16677)
                     /gene="thiE"
                     /locus_tag="OO566_001762"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6605074.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16677..18572)
                     /gene="thiC"
                     /locus_tag="OO566_001763"
     CDS             complement(16677..18572)
                     /gene="thiC"
                     /locus_tag="OO566_001763"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6605075.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIHRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18653..18753)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18806..19282)
                     /gene="rsd"
                     /locus_tag="OO566_001764"
     CDS             complement(18806..19282)
                     /gene="rsd"
                     /locus_tag="OO566_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6605076.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19377..20150
                     /gene="nudC"
                     /locus_tag="OO566_001765"
     CDS             19377..20150
                     /gene="nudC"
                     /locus_tag="OO566_001765"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6605077.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20190..21254
                     /gene="hemE"
                     /locus_tag="OO566_001766"
     CDS             20190..21254
                     /gene="hemE"
                     /locus_tag="OO566_001766"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6605078.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21411..22082
                     /gene="nfi"
                     /locus_tag="OO566_001767"
     CDS             21411..22082
                     /gene="nfi"
                     /locus_tag="OO566_001767"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6605079.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22125..22715
                     /locus_tag="OO566_001768"
     CDS             22125..22715
                     /locus_tag="OO566_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6605080.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22902..23174
                     /gene="hupA"
                     /locus_tag="OO566_001769"
     CDS             22902..23174
                     /gene="hupA"
                     /locus_tag="OO566_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6605081.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23187..23882
                     /locus_tag="OO566_001770"
     CDS             23187..23882
                     /locus_tag="OO566_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6605082.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRASFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23884..24309)
                     /gene="zraP"
                     /locus_tag="OO566_001771"
     CDS             complement(23884..24309)
                     /gene="zraP"
                     /locus_tag="OO566_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6605083.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24547..25923
                     /gene="zraS"
                     /locus_tag="OO566_001772"
     CDS             24547..25923
                     /gene="zraS"
                     /locus_tag="OO566_001772"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6605084.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25920..27245
                     /gene="zraR"
                     /locus_tag="OO566_001773"
     CDS             25920..27245
                     /gene="zraR"
                     /locus_tag="OO566_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6605085.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27242..28531)
                     /gene="purD"
                     /locus_tag="OO566_001774"
     CDS             complement(27242..28531)
                     /gene="purD"
                     /locus_tag="OO566_001774"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6605086.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28543..30132)
                     /gene="purH"
                     /locus_tag="OO566_001775"
     CDS             complement(28543..30132)
                     /gene="purH"
                     /locus_tag="OO566_001775"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6605087.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7195 a         7975 c         8439 g         7058 t
ORIGIN      
        1 ccggtcgtgg taccgttgtt accggtcgtg tagaacgcgg tatcatcaaa gttggtgaag
       61 aagttgaaat cgttggtatc aaagagactc agaagtctac ctgtactggc gttgaaatgt
      121 tccgcaaact gctggacgaa gggcgtgctg gtgagaacgt aggtgttctg ctgcgtggta
      181 tcaaacgtga agaaatcgaa cgtggtcagg tactggctaa gccgggcacc atcaagccgc
      241 acaccaagtt cgaatctgaa gtgtacattc tgtccaaaga tgaaggcggt cgtcatactc
      301 cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg actggtacca
      361 tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa atggttgtta
      421 ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt gaaggcggcc
      481 gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata acatttgacg
      541 caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt accagaacct
      601 ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt tgagggcgta
      661 taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg gggtgaaaat
      721 gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc tcaaggaagc
      781 gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct ggcggcgatt
      841 gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc cgtagtaatt
      901 ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc taccgttgct
      961 tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg ccaggaaaca
     1021 ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat cctgtgggga
     1081 ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt ctgagatgtc
     1141 tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg aaggccgcgt
     1201 agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt ttggtgaagt
     1261 catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca aaagcgaacg
     1321 taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga gctggcactt
     1381 ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc gtcctgcgcc
     1441 aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg gtgataagcc
     1501 gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg gtccgttcgc
     1561 tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga aagtgtctgt
     1621 ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg aaaaagccta
     1681 acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat tggaatacaa
     1741 tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata tcacggggag
     1801 cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa agtacaagcc
     1861 tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt aggtccggct
     1921 ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc aaaaactgat
     1981 tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga ccgttctttc
     2041 actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc tggtatcaag
     2101 tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc tcagctgcag
     2161 gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc gatgactcgc
     2221 tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga aatggctaaa
     2281 ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca gtacgacatc
     2341 aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga aagcgtggac
     2401 gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg tggtgcaact
     2461 gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca aggtgcaaac
     2521 gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct ggctgaccag
     2581 atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc aatgcgcgtt
     2641 gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc gaaagtgggt
     2701 actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca ggttcgttac
     2761 cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt tgacgctgac
     2821 aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa accgactcag
     2881 gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc aggtgttgca
     2941 gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg cggtgatttt
     3001 gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga gatattcggg
     3061 ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg tgactggtgc
     3121 gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa gatttgttcg
     3181 ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca agaaacttaa
     3241 tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg gcttgtttct
     3301 gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc cagagatttt
     3361 ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca aacaagcgat
     3421 tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg cggattcccg
     3481 tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag ctggcgtata
     3541 catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc cgttcgagtg
     3601 cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac acccgggcgc
     3661 tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg tcaaagccgc
     3721 tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa ctctgccgac
     3781 ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg ctggcaaact
     3841 ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc agttatcttt
     3901 ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt aaatgtctat
     3961 cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg ttgtagaact
     4021 gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag ctgtagctgc
     4081 tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga aagctgctgg
     4141 cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg gtctgaaaga
     4201 agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga gcaaagacga
     4261 cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag ttaaataagc
     4321 caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt ttagtcacca
     4381 gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact gctgtgcctt
     4441 tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt tctgtgtaaa
     4501 tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt tccattttcc
     4561 ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt cgacttgtca
     4621 gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt cgtaaggatt
     4681 ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag cttgactcgt
     4741 ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct gctttccgtt
     4801 ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc agctaccgcc
     4861 ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc tattccgcac
     4921 cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa ggcaccgtaa
     4981 aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca gacaacggta
     5041 cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt agtccgggcg
     5101 tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg tataacgcgc
     5161 gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag gacaacctgt
     5221 tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc gccctgaact
     5281 acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa atccgtgata
     5341 acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca tcttttgaca
     5401 tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg cgccacattc
     5461 gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac atcgcaggta
     5521 aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc gcagcgaaca
     5581 tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag cgtatcgaaa
     5641 cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta cgtgtcgacc
     5701 caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc cctggcgagc
     5761 cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc gaagaccgtt
     5821 atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc gaagaaatcg
     5881 aaggttccgg tattctgagc aaagacgaca tcattgatgt tatgaaaaag ctcatcgata
     5941 tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt cgtatccgtt
     6001 ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta gagcgtgcgg
     6061 tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat atgatcaacg
     6121 ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg tctcagttta
     6181 tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc gcactcggcc
     6241 caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac ccgactcact
     6301 acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg atcaactctc
     6361 tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat cgtaaagtga
     6421 ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa ggcaactacg
     6481 ttatcgccca ggcgaactcc aacctggatg aaggaggcca cttcgtagaa gacctggtaa
     6541 cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac tacatggacg
     6601 tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg gaacacgatg
     6661 acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg actctgcgcg
     6721 ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac tccggtgtaa
     6781 ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt atcgttatca
     6841 aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac aacctgacca
     6901 aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg tctctgggtg
     6961 aactggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc ggtgaactgg
     7021 cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac ttcgaagact
     7081 ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc cacattcagg
     7141 aactggcgtg tgtgtcccgt gacaccaagc tgggtccgga agagatcacc gctgacatcc
     7201 cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt tacattggtg
     7261 cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt gaaactcagc
     7321 tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct gacgttaaag
     7381 actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt caggtcttta
     7441 ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg cagctcaaac
     7501 aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg ttcagccgta
     7561 tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa ctgccgcgcg
     7621 atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg gaacagctgg
     7681 ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg aaacgccgaa
     7741 aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag gtatatctgg
     7801 cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt aacaagggtg
     7861 taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt acgccggtag
     7921 acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag atcctcgaaa
     7981 cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg ctgaaacagc
     8041 agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg ggcgctgacg
     8101 ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt ctggctgaaa
     8161 acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa gaagcagaaa
     8221 ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc ctgtacgatg
     8281 gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac atgctgaaac
     8341 tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac agcctggtta
     8401 ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg gagatggaag
     8461 tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc actgttaagt
     8521 ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc aaccatcaga
     8581 tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt tcgctgggta
     8641 tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct gtcggggtaa
     8701 aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga tttattaaag
     8761 tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc tctggcttcg
     8821 ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac catcaactac
     8881 cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg gccggtaaaa
     8941 gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt catctgtgag
     9001 aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg ccacatcgaa
     9061 ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg tatcggtctg
     9121 ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc ctatgtggtt
     9181 atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga gcagtatctg
     9241 gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga agcaatccag
     9301 gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga agagctgaac
     9361 gaaaccaact ccgaaactaa gcgtaaaaag ctgaccaagc gtatcaaact gctggaagcg
     9421 ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc ggtactgccg
     9481 ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc tgacctgaac
     9541 gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct ggatctggct
     9601 gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt agacgccctg
     9661 ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc tctgaaatct
     9721 ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct cggtaagcgt
     9781 gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct gcatcagtgc
     9841 ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg caagctggaa
     9901 ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg cgaagaagct
     9961 gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct gaaccgtgca
    10021 ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga aggtaaagct
    10081 atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg tgaccagatg
    10141 gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct gatgatgtct
    10201 accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc tcaggacgtt
    10261 gtactgggtc tgtactacat gacccgtgac tgtgttaacg ccaaaggcga aggcatggtg
    10321 ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc tctgcatgcg
    10381 cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt agtagcgaaa
    10441 accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt accgaaaggt
    10501 ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa aatgctgaac
    10561 acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca gatcatgtac
    10621 accggcttcg cctatgcagc gcgttctggt gcatctgttg gcatcgatga catggtcatc
    10681 ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga aattcaggag
    10741 cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat cgatatctgg
    10801 gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac tgaaaccgtt
    10861 attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta catgatggcc
    10921 gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat gcgtggtctg
    10981 atggcaaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt ccgtgaaggt
    11041 ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct ggcggatacc
    11101 gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt ggcgcaggac
    11161 ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac tccggttatc
    11221 gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt aactgctgaa
    11281 gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct gctgcacgaa
    11341 cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg ttctgttgta
    11401 tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct ggcgcgtggc
    11461 cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat cggtgaaccg
    11521 ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg tgcggctgct
    11581 gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt gaagtcggtt
    11641 gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa actgatcgac
    11701 gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact ggcgaaaggc
    11761 gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca caccatgccg
    11821 gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg ccagaccatt
    11881 actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga ttccgcagaa
    11941 cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc tcagggtaac
    12001 gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg taaagcgatt
    12061 gttcagctgg aagatggcgt acagatcagc tctggtgaca ccttggcgcg tattccgcag
    12121 gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga cctgttcgaa
    12181 gctcgtcgtc cgaaagaacc ggcaatcttg gctgaaatca gcggtatcgt ttccttcggt
    12241 aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag cgatccgtac
    12301 gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg tgtagaacgt
    12361 ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct gcgtggtgtt
    12421 catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct gcagggcgta
    12481 aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa agctaccatc
    12541 gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc tcgcgtcaag
    12601 atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc ccgcgatctg
    12661 ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc atcgttccag
    12721 gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga actgcgcggc
    12781 ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta cgcgtaccac
    12841 caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca ggtgactgca
    12901 gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg ttctgataac
    12961 gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt tttatgggtg
    13021 ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga aacgaggaag
    13081 aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa acacaacgct
    13141 ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta cgttcttaca
    13201 ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct ttccataccg
    13261 gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga tcgtcgtgcg
    13321 gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc gttttcgaga
    13381 aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac ggtgattccc
    13441 gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag atggtttgca
    13501 ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac gggcgcaggc
    13561 gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat agcaaatgtt
    13621 cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc gcgccgaggc
    13681 ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc cagaagaagt
    13741 cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat gtctcctgat
    13801 aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc tccgccagcg
    13861 gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga tgacgacgaa
    13921 agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga tgttcaaagc
    13981 ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc gttttgcgct
    14041 tgatgcgatt actcatggaa aatccgcagt acgtgcagtt gttagcgcaa agattggaaa
    14101 gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc agacgctgcg
    14161 cctgttgggc cagttgttcc agatagccac tggcggcagg cgataacagc gccatcatgt
    14221 cgtcgcgggt gagttgcgag gcatttagcg cccgctctac gtcagcagcc gttttgccgt
    14281 tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgattgctg aaggttttca
    14341 tgccgatgcc tccagaaatc cggtcagcgg gctggtggca tgagcaaaat aactgcggct
    14401 gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa atgccttcgc
    14461 catgttgacg ggatcgtccg cgacggcaat cgccgtattc actaacaccg cgtcggcccc
    14521 catttccagc gcctgcgcgg catggctggg aacgccgatg ccagcatcga caaccaccgg
    14581 aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc cctgattcga
    14641 gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca gacgtttaca
    14701 caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta ccagcgtttc
    14761 ggcggctttc agggtttcga tgggatcggg caacagccag cgggcgtcag ggtgaatctc
    14821 caattttagc cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga aaatggcttc
    14881 ttccgctgtt ttcgccccgg aagtattcgg cagcagggcc acacccgccg cgataagcgg
    14941 ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca gtgtcaccag
    15001 ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga atttccctgt
    15061 gccggtaaac agatgtgaat caaacgtttt gtccacaata cgtaacatct caaccccctg
    15121 caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc gtccactgct
    15181 cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt tggtcgagtt
    15241 gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga tcgttaaaca
    15301 ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa cgccaggctg
    15361 cgccactggc tcgacttacc gtcgaacagt cggagttctc ccgcaggcgt ctctataccg
    15421 cttaataact taatggcttc cagtgcctgc aaagtgccca tcaccccgac caccgggcca
    15481 accacgcccg ccgtgcggca gttgcgttct ggctgctggt tatctggcca caggcagcgg
    15541 taacacccct gctcccaggg cggtgtcagt accatcaact gaccgccaaa tccgaccgcg
    15601 ctggcggtga taagcggcgt gttgagtgcc acgcaggcgg cattaatctc ctggcgagtc
    15661 gccatattgt cggtacagtc gagcaccaca tcggcttgtg caaccgcatc tttcaacgcc
    15721 tcacccgtta accgttgttg taatgctgtc agttgaatgt cgggattcaa ctgtgtcagt
    15781 cgctgttggc tgacctgcga tttcgggctg ttgatatctg cagtggtaaa gaggatttgt
    15841 cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt cccgacgcca
    15901 gcccctgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat aatcagcacc
    15961 tggctgtcga gcagtttttg ctgcccgtca agagcgatat cgtcgagcag aatttggcgg
    16021 ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca gcaactgtgc
    16081 cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga tactgccgac
    16141 acccgttgct atcaccgcag gcgcgcgtgc cagactgata ccgccaatcg ccacggtggg
    16201 ataatccgcc aggcgctcaa catgccgtgc caactgttcc agcccctgcg gtgcagaagg
    16261 catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg ggcgtgctgc
    16321 cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca ggcctgccgc
    16381 gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga cgccatacgc
    16441 ctgatgcttg atcgcgagcc gccagtaatc gttgataaac aatcgcgcgt tatagcggcg
    16501 gcccagcgca attgccgcca cgacatcggc ttccacctct tcatcgcgcc gatctttgat
    16561 gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact gtacgctgtc
    16621 caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct gatacatcac
    16681 gcttcctcct tatgcaggta gatttctccg cctctggcac ggaagttctc cgacatatcc
    16741 gccattccca cttcaatggt ttgcgcggcg gcgtaatcgc gtacttcctg gctgattttc
    16801 atcgagcaga attttggccc acacatggag caaaaatggg cgactttgcc ggactcttgg
    16861 ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag attaaactgg
    16921 tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat ttgcgcgccc
    16981 ggatgccctt ttgccaggtc agcagcgtgg gcggcaatct tataagtgat aagcccctgc
    17041 ttaacatctt ctttattggg cagacccaga tgctcttttg gcgttacgta acagagcatc
    17101 gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg gtcatagccc
    17161 ggtgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg gcagtgctct
    17221 aactcctcgg tcatattgcg gtggatcatc tgcatcggca catggcctgg gccttcaatc
    17281 atcacctgta cgtcatattc ccaggcgatt ttggtcagtt cgcccagcgt atgcaattcg
    17341 gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc gtcgcccagc
    17401 gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg ttgatagagg
    17461 aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc gcgagagacg
    17521 ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc cgcatggata
    17581 gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa cgcttcccag
    17641 gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg cactgtaccg
    17701 atcggcaccg gactgttacg caaaatccac tcgcgggttt cgtgaatata gcgaccggtg
    17761 gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt ttccacttct
    17821 tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt taccaggaag
    17881 ttacggccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg gataatcgca
    17941 cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg cagatgtgcg
    18001 ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg ctcgcggccc
    18061 atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg gcgggcgtag
    18121 tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa gccgctaaaa
    18181 cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga actgcgcacg
    18241 gtaagttctt cggtatcggc gcgcgcatcg atccacggct ggcgtagttt tgccagccct
    18301 tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc gtagaccgga
    18361 atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag cgtcgggcta
    18421 agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat ataaatgcgt
    18481 tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc ccgggcgcgt
    18541 tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtt aaggacgtgg
    18601 cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg atagcagatt
    18661 aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc gaataaacgg
    18721 tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg cgttgaatgt
    18781 aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg gcgtcaagca
    18841 ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg ccgatgtcag
    18901 ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc aggctggaat
    18961 cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc ttcgcggcgc
    19021 gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata ctgaaatgtc
    19081 cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt tcgtttagcc
    19141 tcatgtacga ttctttgcca ggcttaatgc caaccagatt gtagtaagcc acgagcagat
    19201 gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg cgctccgtca
    19261 ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa actgctcagt
    19321 gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca agagctatgg
    19381 atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa caaaaattat
    19441 ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt gtgggtcagc
    19501 gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttaatacaa cagcagcggc
    19561 gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg tttcaactgg
    19621 ccggacgtgg cgtgcaactg gcggagtttt accgatcgca taaatactgt ggttactgcg
    19681 ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat tgccgtgagc
    19741 gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat gattcgatcc
    19801 tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc ggattcgtcg
    19861 aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag agcggaatta
    19921 aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag tctttaatga
    19981 ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa gaattgctcg
    20041 aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc accgtagcgc
    20101 gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga tgatacactg
    20161 accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat cgttatctgc
    20221 gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc caggcgggtc
    20281 gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg tcgctgtgca
    20341 aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac ccgctggatg
    20401 cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta gggctctatt
    20461 ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc gacgtcgata
    20521 aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg gtgcgtacca
    20581 ttcgtcacga actgaaaggc gaagtgccgc tgattggttt ttccggcagc ccgtggacgc
    20641 tggcaaccta catggtggaa ggcggcagca gcaaagcctt caccgtgatc aaaaaaatga
    20701 tgtatgccga tccgcaggcg ctgcacgctc tgctcgataa actggcgaaa agcgtcactt
    20761 tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc gacacctggg
    20821 gcggcgtgct taccgggcgc gattatcaac agttctcgct ctattacatg cataaaattg
    20881 ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg tttaccaaag
    20941 gcggcggaca gtggctggaa gccatggctg aaaccggttg cgatgcgctg ggcctcgact
    21001 ggacaacgga catcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg ttgcagggta
    21061 atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa gtagcgacta
    21121 tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac ggcattcatc
    21181 aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga ctgtctgaac
    21241 agtatcaccg ctaagagcct gatccatcag gctattttat ttgccatttt gaggccgggc
    21301 agtgctcaaa atcctcacgt actacgtgta cgctccggtt tttccgcgct gtccggcctc
    21361 aaactgcctt caacaattac gcctgttgaa ccaggctcta aggagcgatt atggatctcg
    21421 cgtcattacg cgctcaacaa atcgaactgg cttcttctgt gatctgcgag gatcgactcg
    21481 ataaagatcc accggatctg atcgccggag ccgatgtcgg gtttgagcag ggcggagaag
    21541 tgacgcgagc ggcgatggtg ctgctgaaat atccctcgct tgagctggtg gagtataaag
    21601 ttgcccgcat cgccaccacc atgccttaca ttccaggttt tctttccttc cgcgaatatc
    21661 ctgcgctgct ggcagcgtgg gagatgctgt cgcaaaagcc ggatttagtg tttgtcgatg
    21721 gtcatgggat ctcgcatcct cgccgtcttg gcgtcgccag ccattttggc ttattggtgg
    21781 atgtgccgac cattggcgtg gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca
    21841 gcaaaccggg cgcgctggcc ccactgatgg ataaaggcga gcagctggcc tgggtctggc
    21901 gcagcaaagc gcgctgtaac ccgttgttta tcgctaccgg ccatcgggtc agcgtggaca
    21961 gcgcgctggc gtgggtacaa cgctgcatga aaggctatcg tctgccggag ccaacgcgct
    22021 gggcggacgc ggtggcctcg gaacgtccgg cgttcgtgcg ctatacagca aatcagccct
    22081 aattcaggta aactgcggat aatttccgta tttgagaact caacatgtta caaaacccaa
    22141 ttcatctgcg tctggagcgc ctggaaagct ggcagcatgt cactttcatg gcttgcttat
    22201 gcgaacgcat gtaccccaat tacgccatgt tctgccagca aaccggtttt ggtgatgggc
    22261 aaatttaccg tcgtattctc gatctcatct gggaaacgct gaccgttaaa gacgcaaaag
    22321 taaatttcga cagccaactg gagaaatttg aagaagcgat tccttcagcc gacgatttcg
    22381 atctgtacgg cgtttatccg gcaatcgatg cctgcgtggc gttaagtgaa ctggtccatt
    22441 cgcgtttgag tggtgaaacg ctcgaacacg cggtggaagt gagtaagacc tccatcacga
    22501 ccgttgcgat gctggaaatg actcaggctg gtcgcgaaat gagcgatgaa gagcttaaag
    22561 aaaacccagc tgtagagcaa gaatgggaca ttcagtggga aatattccga cttttagctg
    22621 agtgcgaaga acgcgatatc gagctgataa aaggccttag ggcagacctg cgtgagtcgg
    22681 gtgagagcaa tattggtata atttttcagc aataagacca gaaaacgtga tttaacgcct
    22741 gatttgtcgt acctggagtc ttccctttcg ccccccgtct ggtctacatt tggggggcga
    22801 aaaaaagtgg ctatcggtgc gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac
    22861 tcgatgctta gcaagcgata aacacattgt aaggataact tatgaacaag actcaactga
    22921 ttgatgtaat tgcagagaaa gcagaactgt ccaaaaccca ggctaaagct gctctggagt
    22981 ccactctggc tgcaattact gagtctctga aagaaggcga tgctgtacaa ctggttggtt
    23041 tcggtacctt caaagtgaac catcgcgctg agcgtactgg ccgcaacccg cagaccggta
    23101 aagaaatcaa aattgccgca gctaacgtac cggcatttgt ttctggcaag gcactgaaag
    23161 acgcagttaa gtaagattgc gtggcagtga acagttttaa cgaaggggtg gtttcacccc
    23221 ttttgtcttt ctggcgtcga tcattgatgc tggcaggtgc gctgtttctc actgcctgta
    23281 gccacaactc ttcacttcct ccctttaccg ccagtggatt tgctgaagac cagggcgcgg
    23341 tacgcatctg gcgaaaagac agcggcgata atgtgcatct gcttgccgtg tttagcccgt
    23401 ggcgcagtgg cgataccacg acgcgagagt atcgctggca gggcgataac ctcacgctca
    23461 tcaatatcaa tgtttacagc aaaccgccgg tgaacattcg cgcgagtttt gacgatcgcg
    23521 gcgatctgag ctttatgcaa cgtgaatccg atggggaaaa gcagcagctt tctaacgacc
    23581 aaatcgattt ataccgttat cgtgctgatc agatccgcca gattagcgat gccttacgtc
    23641 aggggagagt cgtgctgcgc caggggcgct ggcatgcgat ggaacagacc gtgaccacct
    23701 gcgaagggca aaccattaaa cctgatttag attcgcaggc gatagcgcat atcgagcgcc
    23761 gccagagccg ctcttctgtt gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc
    23821 aattactgtt agtggcaaac tctgatttct gtcgctggca acccaacgag aaaacgttct
    23881 gatttaccag tggcccatac ccatatgacc gccaccaccg cagccgccgt agcccatgcc
    23941 cattccggct ccgcgcggaa tacccgcttc agccatcgcg atatctcgtt tcacccgtaa
    24001 ctcatctaac gactgacgca aattctccat ctctttggcg accgcgttaa ttttgctgct
    24061 atccggtggg ttcgcggcta acagggcatt gtattcataa cgcttcgtca ccagttgctg
    24121 ttgcagtgcg ctgctttgag cgtaaaagtc attatggatt ttctgccacg ctgtctgttg
    24181 ttcgctggtc aaaggcgcgg cattttgctg ccacataccg tgtccgccgt gagcaaatgc
    24241 agatgtcgat cccatcgcca ttgctgaaag cgccatcatt accagggcaa ttttcgtgtt
    24301 ccgtttcatg gttaatcctc cagtggttgt cttacttcgg gtattgcatc ttccgtgcca
    24361 acgatgaaac gctgatatga cgggtaatct ggcatgataa acgagtaaaa atgactcgcc
    24421 tgctgcgggt agcgagtcat ttttactcat tgaaacttaa gcctttgtgt tacagcgcag
    24481 ggtaagcgct gataaaagat ggcatgattt ctgctgtcag aaagggatga gcaggcaaag
    24541 aagaagatgc gttttatgca acgttctaaa gactccttag ctaaatggtt aagcgcgatc
    24601 ctccccgtgg tcattgttgg gctggtaggg ttgtttgcgg tgacggtgat tcgcgattat
    24661 gggcgcgaga ctgccgccgc cagacaaacg ctgctggaaa aaggcagtgt acttattcgc
    24721 gctcttgaat ccggctcgcg cgtcggcatg gggatgcgca tgcatcatgc gcagcagcag
    24781 gccttactgg aagaaatggc cgggcagcct ggagtacgtt ggtttgcggt cacggatgaa
    24841 caaggaacaa tcgtgatgca tagcaactcc ggcatggtgg gaaaacagct ttattcccct
    24901 caggaaatgc agcagttaca tccgggagat gaagaagcgt ggcggcggat cgatagcgca
    24961 gacggcgagc ctgttctgga aatttatcgc cagtttcaac cgatgtttgc tgctggaatg
    25021 caccggatgc gccatatgca gcagtatgcc gcgacaccac aagcaatttt catcgctttc
    25081 gacgccagta atattgtgag tgccgaagat cgtgagcaga gaaacaccct gattattctc
    25141 ttcgccctgg cgacggtctt gctggcaagc gtattgtcat tcttctggta tcgccgctat
    25201 ctgcgctcgc gccagcttct acaagatgaa atgaagcgca aagagaagct ggtggcgctg
    25261 gggcatcttg cggcaggcgt tgcccacgaa atccgtaacc cactttcctc gattaaagga
    25321 ctggcgaaat actttgccga gcgcgcgcct gcagggggag aagcgcatca actggcgcag
    25381 gtgatggcga aagaggccga ccgtttaaac cgcgtggtaa gcgagttgct ggaactggtt
    25441 aagccaacgc atctggcttt gcaggcggtg gatctcaaca cgctgattaa ccactcatta
    25501 cagctggtaa gccaggatgc aaacagccgg gagatccagt tacgctttac cgccaacgac
    25561 acattaccgg aaattcaggc cgatccggac aggctgactc aggtcctgtt gaatctctat
    25621 ctcaatgcta ttcaggcgat tggtcagcat ggcgtgatta gcgtgacggc cagcgaaagc
    25681 ggcgcgggcg tgaaaatcag cgttaccgac agcggtaagg gaattgcggc agatcagctt
    25741 gaagccatct tcactccgta cttcaccacc aaagccgaag gcaccggatt ggggctggcg
    25801 gtcgtgcata atattgttga acaacacggt ggtacaattc aggtcgcaag ccaggaggga
    25861 aaaggctcaa cgttcaccct ctggcttccg gtcaatatta cgcgtaagga cccacaagga
    25921 tgacgcacga taatatcgat attctggtgg tggatgatga cattagccac tgcactattt
    25981 tgcaggcttt actgcgcggc tggggctata acgtcgcgct ggcgaacagc gggcgacagg
    26041 cgctggagca ggtgcgggaa caggtttttg atcttgtgct ttgcgatgtg cgaatggcgg
    26101 agatggacgg catcgccacg ctgaaagaga tcaaagcgtt aaacccggca attccggtgc
    26161 tgattatgac tgcgtactcc agcgtcgaga cggcggtaga ggcactgaaa actggggcgc
    26221 tggattatct catcaagccg ctggatttcg ataacctaca ggcgacgctg gaaaaggcgc
    26281 tcgcgcatac gcacagtatt gatgctgaaa cacctgcggt gactgccagc cagttcggta
    26341 tggtcggtaa aagcccggcg atgcaacacc tgctcagtga aatcgccctc gtcgcgccat
    26401 cggaagccac ggtgctgatc cacggcgatt ccggcaccgg taaagagctg gtcgccaggg
    26461 cgattcacgc cagtagcgca cgtagcgaaa aaccgctggt aacgctcaac tgtgcggcac
    26521 tcaacgaatc cttgctggaa tctgaattgt tcggtcacga aaaaggggcg tttactggag
    26581 ccgataaacg ccgggaaggg cgctttgttg aggcggacgg cggcacgctg tttctcgatg
    26641 aaattggcga tatctcgccg atgatgcagg tgcgtctgct gcgtgcgatt caggagcgcg
    26701 aagttcagcg tgtcggtagc aaccagacta tctcggttga tgtccggctg attgcggcga
    26761 ctcatcgcga tcttgccgca gaggtgaatg ccgggcgttt tcgccaggat ctctactatc
    26821 gcctgaatgt ggtggcgatt gaagtaccat cgctgcggca acggcgggaa gatatccctc
    26881 tgctggctgg ccattttctt cagcgctttg ccgagcgtaa tcgcaaggcg gtaaaaggct
    26941 ttacgccaca ggcgatggat ttgctgatcc actacgactg gccgggaaat attcgtgaac
    27001 tggaaaacgc ggtggaacgg gcggtggtgc tgctgaccgg ggaatatatt tccgaacgcg
    27061 agctgccgct ggcgattgcc agtacgccga tcccgctggg gcaaagtcag gatattcagc
    27121 cgctggtgga agtggaaaaa gaggtgattc tggcggcgct ggagaaaacg ggcggcaaca
    27181 aaaccgaagc cgcccgtcag ttagggatca cgcgcaaaac gctattggca aaactgtcgc
    27241 gttagttctg ctcgcgttcg atggcgcgcc aaccgatatc tttccggcag aagcaatcat
    27301 cccagtggat atcggtcatt aaggcgtagg cgcgtttctg cgcttctgcc acggtatgac
    27361 ccagcgcggt gacgcacagt acgcgcccgc cgctggttac cacctgctcg tcatccgcca
    27421 gttttgtgcc cgcgtggaac actttgccgt cagccacttc ttccagcggc aggccgtgga
    27481 tcacatcgcc ggtgcggtaa tcgcccggat atccacccgc agccatcacc acgctgagag
    27541 aagcacgttc atcccactct gacgttttct cgtccagttt gccttcacag gccgccaggc
    27601 agagctcaac cagatcggac ttcatgcgca gcataatcgg ctgggtttcc ggatcgccaa
    27661 agcggcagtt aaattcaata accttcggat tgccctgttt gtcgatcatc aggcccgcat
    27721 agagaaaacc ggtgtaggtg ttgccttccg ccgccatgcc tttcacggtt ggccagatga
    27781 tacgttccat ggtgcgctga tgaacttcat cggttactac cggcgcgggg gagtaagcgc
    27841 ccatcccgcc ggtgtttggt ccgttatctt tatcgcctac gcgtttgtga tcctggctgg
    27901 tagccatcgg cagcacatgc tcgccgtcca ccatcacgat aaagctcgct tcttcgccat
    27961 cgaggaactc ttcgataacg atgcgatgac ccgcgtcgcc aaaagcgttg cccgccagca
    28021 tatcgtgaac agccgcttcc gcttcttcca gcgtcatcgc cacgataacg cctttcccgg
    28081 cagccagacc gtccgcttta atgacgattg gcgcgccttt ctcacgcaga tacaccagcg
    28141 caggttctac ctcggtgaag ttctggtatt ccgccgtagg gatcttatgg cgggccagga
    28201 aatctttggt aaacgctttt gagccttcca gttgggccgc acctgcggtt gggccgaaga
    28261 ttttcagccc ggcggcgcgg aaggtatcga ccacgccttt caccagcggc gcttccgggc
    28321 cgacgatggt cagatcaatc ttttcgtttt gtgcgaaatc caacagcgcc gggatatcgg
    28381 tcacgccgat agcaacgttt tgcagcgcgg gttccagcgc agtgcctgca ttacccggag
    28441 caacaaaaac agtctcaacc agcggcgact gggccgcttt ccaggccagc gcgtgctcgc
    28501 gcccgccgtt accaatcact aatactttca tctattgctc cattaatggc ggaagtggcg
    28561 catgtcggtg aagagcatcg caataccgtg ctcgtcggcg gcggcaatca cttcgtcatc
    28621 acggatggaa ccgccaggct ggattacgca ggtcacgccc gcagcggcgg cggcatcaat
    28681 accgtcgcgg aacgggaaga acgcgtcaga agccatcgag gaacctttca cttccaggcc
    28741 ttcatcggca gctttaatac cggcgatttt cgcggagtac acgcggctca tctggcccgc
    28801 gccaataccg atggtcatat tgtttttggc atagacgata gcattggatt tcacgaattt
    28861 cgccactttc cagcagaaca gcgcatcacg cagttcctgt tcggtcggct gacgttgggt
    28921 gacgacgcgc agttcttccg caccgaccat ccccaggtca cgatcctgaa ccagcagacc
    28981 gccgttcacg cgtttgaaat caagacccgg aacacgctcg ccccactgac cgcaggtcag
    29041 aacgcgtacg ttctgcttgg cggcggtgat tttcagggct tcttcgctgg cggaaggcgc
    29101 aataatcact tcgacaaact gacgagaaat gatggcctgt gcggtttccg catccagctc
    29161 gcggttaaag gcaatgatgc cgccgaatgc ggaggttggg tcggttttgt acgcgcgatc
    29221 gtaagcatca agaatggaat tgccgatagc tacaccgcaa gggttggcgt gcttcacaat
    29281 cacacatgcc ggctcggcga actctttcac gcactccagc gccgcatcgg tatcggcgat
    29341 gttgttataa gagagggctt taccctgaac ctgggttgcg gtagcaacgg aggcttcttt
    29401 cacattctct tctatataga aggcagcctg ctggtggctg ttctcgccgt aacgcatatc
    29461 ctgcttctta atgaagttca ggttcagcgt gcgtgggaag cgaccggcgg cttctttgct
    29521 ttcaccgtgg tacgccggaa ccatgctgcc gaagtagttg gcaatcatgc tgtcgtaggc
    29581 ggcagtgtgt tcgaaggctt tgatggcgag gtcgaaacgg gttgcaagcg tcagcgatcc
    29641 ttcgttgtca tccatctctt taataatggc gtcgtagtcg ctgctcttca ccacgattgc
    29701 gacatcttta tggttcttag cggcggagcg caccatggtc gggccgccga tatcgatgtt
    29761 ctcaaccgca tcttccagcg agcagccttc acgagcaaca gtctgggcga acggatacag
    29821 gttaacaacc accatatcga taggctggat ctgatgttct tccataatgg catcgtcctg
    29881 gccgcgacgg cccagaatgc caccatgtac tttcggatgc agggtcttca cgcgtccatc
    29941 catcatctcc gggaaaccgg tgtaatcgga aacttcggtt accggcagac ctttttctgc
    30001 taacagacgg gcagtgcccc ctgtagacag cagctccaca ccgcgtgcgg aaagtgcctg
    30061 ggcgaattcg acgataccgg ctttgtcaga aacactgagc agagcgcggc ggactggacg
    30121 acgttgttgc atggtaaatc ccttgggttt gactattaca gagagcgtta gctgaatttt
    30181 tcgcgaaaaa ctcagctaac gcccctaacg gggcatcctt atttttcgcc cgcattgtaa
    30241 cgaaaacgtt tgcgcaacgc tcgcgaattt ttctctttca atggtgatca caattttgac
    30301 agtggttacc gtgggaaaaa tacagaaatt acattgatga ttgtggataa ctctgtgcgt
    30361 aaaaaggtat aaagcggggt tttgctgggg aatgcagcag tcagtcattt ttctgcaatt
    30421 tttctattgc ggcctgcgga gaactcccta taatgcgcct tcatcgacac ggcggatgtg
    30481 aatcacttca cacaaacagc cggttcggtt gaagagaaaa atcctgaaat tcagggttga
    30541 ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca gtgagctgaa agccgcgtcg
    30601 caactgctct ttaacaattt atcagacaat ctgtgtgggc actcgaagat acggattctt
    30661 aacgtcg
//
LOCUS       DAJTXY010000037        29923 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTXY010000037 DAJTXY010000000
VERSION     DAJTXY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.037"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            217..1665
                     /gene="uacT"
                     /locus_tag="OO566_001776"
     CDS             217..1665
                     /gene="uacT"
                     /locus_tag="OO566_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6605088.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1667..1792
                     /locus_tag="OO566_001777"
     CDS             1667..1792
                     /locus_tag="OO566_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605089.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1789..1860)
                     /locus_tag="OO566_001778"
     CDS             complement(1789..1860)
                     /locus_tag="OO566_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605090.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            1915..2463
                     /gene="idi"
                     /locus_tag="OO566_001779"
     CDS             1915..2463
                     /gene="idi"
                     /locus_tag="OO566_001779"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6605091.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            complement(2506..4023)
                     /gene="lysS"
                     /locus_tag="OO566_001780"
     CDS             complement(2506..4023)
                     /gene="lysS"
                     /locus_tag="OO566_001780"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6605092.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4033..5131)
                     /gene="prfB"
                     /locus_tag="OO566_001781"
     CDS             complement(join(4033..5055,5057..5131))
                     /gene="prfB"
                     /locus_tag="OO566_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6605093.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5222..6955)
                     /gene="recJ"
                     /locus_tag="OO566_001782"
     CDS             complement(5222..6955)
                     /gene="recJ"
                     /locus_tag="OO566_001782"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6605094.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(6961..7671)
                     /gene="dsbC"
                     /locus_tag="OO566_001783"
     CDS             complement(6961..7671)
                     /gene="dsbC"
                     /locus_tag="OO566_001783"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6605095.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(7696..8592)
                     /gene="xerD"
                     /locus_tag="OO566_001784"
     CDS             complement(7696..8592)
                     /gene="xerD"
                     /locus_tag="OO566_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6605096.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8704..9225
                     /gene="fldB"
                     /locus_tag="OO566_001785"
     CDS             8704..9225
                     /gene="fldB"
                     /locus_tag="OO566_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6605097.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9265..9672)
                     /locus_tag="OO566_001786"
     CDS             complement(9265..9672)
                     /locus_tag="OO566_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6605098.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9653..9919)
                     /gene="sdhE"
                     /locus_tag="OO566_001787"
     CDS             complement(9653..9919)
                     /gene="sdhE"
                     /locus_tag="OO566_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6605099.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            10162..11142
                     /gene="ygfZ"
                     /locus_tag="OO566_001788"
     CDS             10162..11142
                     /gene="ygfZ"
                     /locus_tag="OO566_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6605100.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11338..11997)
                     /locus_tag="OO566_001789"
     CDS             complement(11338..11997)
                     /locus_tag="OO566_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6605101.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12161..12472)
                     /locus_tag="OO566_001790"
     CDS             complement(12161..12472)
                     /locus_tag="OO566_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR6605102.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            12517..13949
                     /gene="bglA"
                     /locus_tag="OO566_001791"
                     /pseudo
     CDS             12517..13949
                     /gene="bglA"
                     /locus_tag="OO566_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
     gene            complement(14006..14749)
                     /locus_tag="OO566_001792"
     CDS             complement(14006..14749)
                     /locus_tag="OO566_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6605103.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15016..17889)
                     /gene="gcvP"
                     /locus_tag="OO566_001793"
     CDS             complement(15016..17889)
                     /gene="gcvP"
                     /locus_tag="OO566_001793"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6605104.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18008..18397)
                     /gene="gcvH"
                     /locus_tag="OO566_001794"
     CDS             complement(18008..18397)
                     /gene="gcvH"
                     /locus_tag="OO566_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6605105.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18421..19515)
                     /gene="gcvT"
                     /locus_tag="OO566_001795"
     CDS             complement(18421..19515)
                     /gene="gcvT"
                     /locus_tag="OO566_001795"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6605106.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLIVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            19806..19889
                     /locus_tag="OO566_001796"
     CDS             19806..19889
                     /locus_tag="OO566_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605107.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            complement(19963..21165)
                     /gene="ubiI"
                     /locus_tag="OO566_001797"
                     /gene_synonym="visC"
     CDS             complement(19963..21165)
                     /gene="ubiI"
                     /locus_tag="OO566_001797"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6605108.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(21188..22366)
                     /gene="ubiH"
                     /locus_tag="OO566_001798"
                     /gene_synonym="visB"
     CDS             complement(21188..22366)
                     /gene="ubiH"
                     /locus_tag="OO566_001798"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6605109.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22363..23688)
                     /gene="pepP"
                     /locus_tag="OO566_001799"
     CDS             complement(22363..23688)
                     /gene="pepP"
                     /locus_tag="OO566_001799"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6605110.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23714..24292)
                     /gene="ygfB"
                     /locus_tag="OO566_001800"
     CDS             complement(23714..24292)
                     /gene="ygfB"
                     /locus_tag="OO566_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6605111.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24460..24789
                     /gene="zapA"
                     /locus_tag="OO566_001801"
     CDS             24460..24789
                     /gene="zapA"
                     /locus_tag="OO566_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6605112.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24831..25014
                     /gene="ssrS"
                     /locus_tag="OO566_001802"
     ncRNA           24831..25014
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO566_001802"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25035..25637
                     /locus_tag="OO566_001803"
     CDS             25035..25637
                     /locus_tag="OO566_001803"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6605113.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25734..25793)
                     /locus_tag="OO566_001804"
     CDS             complement(25734..25793)
                     /locus_tag="OO566_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6605114.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26126..26185)
                     /gene="ibsC"
                     /locus_tag="OO566_001805"
     CDS             complement(26126..26185)
                     /gene="ibsC"
                     /locus_tag="OO566_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6605115.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26414..27646)
                     /gene="serA"
                     /locus_tag="OO566_001806"
     CDS             complement(26414..27646)
                     /gene="serA"
                     /locus_tag="OO566_001806"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6605116.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(27902..28561)
                     /gene="rpiA"
                     /locus_tag="OO566_001807"
     CDS             complement(27902..28561)
                     /gene="rpiA"
                     /locus_tag="OO566_001807"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6605117.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            28944..29837
                     /gene="argP"
                     /locus_tag="OO566_001808"
     CDS             28944..29837
                     /gene="argP"
                     /locus_tag="OO566_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6605118.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
BASE COUNT         7109 a         8061 c         7501 g         7252 t
ORIGIN      
        1 ttaatgaaat atcaatatga gaaaattaca gataattcca ttgtcaggtt ctcttgtcat
       61 tttcgtggtt taagcacgat tatgtgattg cctccgcaat tcctgtctct aactcccctt
      121 cctcgcaaaa actggcactc cacgagcatg tgtttagaca gtttcattaa cgtaaacggt
      181 tgctttttat tctggcgggc gaaaggagaa acactgatga gcgccataga ttcccaactt
      241 ccctcatctt ctgggcaaga ccgcccaact gatgaggttg accgcatatt atcaccagga
      301 aagctgatca tactcggtct gcaacacgtc cttgtcatgt acgcaggtgc agtcgctgtt
      361 cctcttatga ttggtgaccg actgggcctc tcaaaagatg ctattgcgat gctcattagc
      421 tcggatctct tttgctgcgg gatcgtcaca ttattgcaat gtatcggtat cggccgcttt
      481 atggggatcc gcctgccggt gattatgtcg gtgacctttg ctgctgtaac accaatgata
      541 gccattggga tgaacccgga tatcggcctg ctggggatat ttggtgccac tatcgccgcg
      601 ggttttatca ccacattatt agcgccactt attggtcgct tgatgccttt attcccgcca
      661 ctggttaccg gtgtggttat tacttctatt gggcttagca tcattcaggt gggtattgac
      721 tgggccgctg gaggtaaagg gaatccgcaa tatggtaatc ccgtttattt aggtatctcc
      781 tttgccgtct taatttttat cttgctcatt actcgctatg cgaaaggatt tatgtccaac
      841 gtcgccgtat tactggggat tgtatttggc tttttacttt cgtggatgat gaatgaagtc
      901 aatttatccg ggctacatga tgcctcgtgg tttgcgattg tcaccccgat gtcgtttggt
      961 atgccgattt tcgatcccgt ttccattctg accatgactg ccgtgttaat catcgtgttt
     1021 atcgagtcga tggggatgtt cctggcactg ggtgaaatag tcggtcgtaa actctcttca
     1081 cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga caatgatagg cggcacgttt
     1141 aacagcttcc cccacacgtc attttctcaa aacgttggcc tggttagcgt gatgcgcgtt
     1201 catagccgct gggtgtgtat ttcttcggga attatattaa tcctgtttgg catggtgcca
     1261 aaaatggcgg tgctggtagc ctccattccg caatttgtgc tgggcggcgc tggtctagtg
     1321 atgttcggca tggtactggc gacagggatt cgaattctgt cgcgctgtaa ctacaccacc
     1381 aaccgttaca acctctatat tgtggcgatc agtctcggcg ttggcatgac tccgacgctc
     1441 tctcacgatt tcttttctaa gttaccggcc gtactgcaac cgctgctaca tagcggcatt
     1501 atgctcgcaa cccttagcgc cgttgtgctg aacgtcttct ttaatggcta tcagcatcat
     1561 gctgacctgg tgaaggaatc cgtctctgat aaagatttaa aagtcaggac agtacgtatg
     1621 tggcttctga tgcgcaagct gaagaaaaat gagcatggag aataatatga attttttaat
     1681 gcgcgctata ttcagtctgt tgttgctttt tactctctct attcctgtca tttctgactg
     1741 tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg ctactttttt aaatggtttt
     1801 tacctgtcgg catccgctca aaacgggcgg ttgtcgataa acgctctctt ggttaatcat
     1861 ttcactcttc aattatctat aatgatgagt gatcagaatt acatgtgaga aattatgcaa
     1921 acggaacacg tcattttatt gaatgcacag ggagttccca cgggtacgct ggaaaagtat
     1981 gccgcacaca cggcagacac cctcttacat ctcgcgttct ccagttggct gtttaatgcc
     2041 aaggggcaat tattagttac ccgccgcgca ctgagcaaaa aagcgtggcc tggcgtgtgg
     2101 actaactcgg tttgtgggca cccacaactg ggagaaagca gcgaagacgc ggtgatccgc
     2161 cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg aatctatcta tcctgacttt
     2221 cgctaccgcg ccaccgatcc gagtggcatt gtggaaaatg aagtgtgtcc ggtatttgcc
     2281 gcacgcacca ccagtgcgtt acagatcaac gatgatgaag tgatggatta tcaatggtgt
     2341 gatttagctg atgtattacg cggtattgat gccacgccgt gggcgttcag tccgtggatg
     2401 gtgatgcagg cgacaaatcg cgaagccaga atacgattat ctgcatttac ccagcttaaa
     2461 taaaaaaccc cgacatttgc cggggttgtg agcataacgt aatgcttatt tcaccggacg
     2521 catcgccggg aacagaataa cgtcgcggat ggtatggctg ttggtgaaca gcattaccat
     2581 acggtcgatg ccaatcccca gacctgctgt cggcggtaag ccatgttcca gtgcggtaac
     2641 gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg tctttcgcgg caacctgatc
     2701 caggaagcgt tgcgcctgat cttccgcgtc attcagctcg ctaaagccgt taccgatttc
     2761 acgaccaccg atgaagaact caaagcggtc tgtgatttcc gggttaatgt cgttacgacg
     2821 cgccagcgga gaaacttctg ccggatattc agtaatgaag gtcggctgaa tcagatgtgc
     2881 ttctgccact tcttcgaaga tctcggtaac gatacggccc agaccccagc tcttctcaac
     2941 gtggataccg atagattcag caatcgcttt cgcagagtcg aagttatcca gatccgccat
     3001 gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg gtcagttttt cgaacggttt
     3061 accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc ttaccgagaa tatcctgtgc
     3121 cagagtacgg aacagcgatt cggtcagctc gatcagatct ttgtaatctg cgtaagccat
     3181 gtagagttcc atcatggtga actctgggtt atgacgtacg gaaatacctt cgttacggaa
     3241 gttacggttg atttcgaata cacgctcgaa gccaccgacc accagacgct tgaggtacag
     3301 ttccggcgcg atacgcaggt acatgtcgag atccagcgcg ttatggtggg tgataaacgg
     3361 acgcgcagcg gcaccgcctg ggatcacctg catcatcggc gtttcaactt ccataaagcc
     3421 acggttcacc atgaactggc gaataccaga gaggatctgc gagcgtactt taaaggtgtt
     3481 acgggattca tcgttagaga tgagatccag ataacgctga cgatagcgcg cttcctgatc
     3541 ctgcaagccg tggaatttat ccggcagcgg acgcagtgct ttggtcagca gacgcagctc
     3601 ggtacagtgg atagacagtt cgccggtttt ggttttgaac agtttacctt tcgcgccgag
     3661 gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa acgccttccg gcagatcgtc
     3721 acgggcaacg tacagctgaa tgcggccacc cacatcctgc agggtaacga aagacgcttt
     3781 acccataata cggcgggtca tcatgcggcc agcaacggcg acttcgatgt tcagcgcttc
     3841 cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat tggtcagagg tatgatcgcg
     3901 acggaaatcg ttcgggaagg caattccctg ctcacgcagg ttcgccagct tctcacgacg
     3961 cgttttcagt tcattgttaa gatcgactac cgcgtcagcg ccctgtgcgt gttgttcaga
     4021 catgttggtt cctcataacc ctgctttcaa acttgcttcg ataaattgat ccaggctgcc
     4081 gtccagcacg gcctgcgtgt tgcgagtttc taccccggtg cgcagatctt taatgcggga
     4141 gtcatcaagg acataagaac gaatctggct gccccagccg atgtcagatt tgttatcttc
     4201 catcgcctgt ttctcggcat ttttcttctg catctccagt tcataaagct tcgctttcat
     4261 ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg tcgttctggc actgggtcac
     4321 gatcccggtc gggatgtggg taatacgcac cgcagattcg gtacggttaa cgtgctgacc
     4381 gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc gccgggttga tttcgatatc
     4441 aatatcatca tcaacttccg gataaacaaa cgcagagctg aacgacgtgt ggcgacgacc
     4501 gccggagtca aacgggcttt tacgcaccag gcggtgaacg cccgtttctg tacgcagcca
     4561 gccgtaagcg taatcgccgg agattttgat cgtcacggat ttaatacccg ccacttcacc
     4621 ttccgactct tcgatgattt cagttttgaa accacgcgat tctgcccagc gcagatacat
     4681 acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg ccagaccccg cctgaatatc
     4741 gaggtaacag tcggcgctgt catattcgcc agagaacata cggcggaact caagctgcgc
     4801 cagtttttct tccagggcgt cgagttcagc aacggcttcg ttaaaggttt cttcgtcgtc
     4861 agcttctaca gccagttcca gcagaccaga aacatcttcc agcccctgtt tcatttggtc
     4921 gagggtgtcg acaacggctt cgagggagga acgctcttta cccagcgcct gtgcgcgttc
     4981 gggttcgttc cagacatccg gctgttccag ctcggcgttt acttcttcca gacgctcttt
     5041 cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga gcgttccgtg aggtcctgaa
     5101 tgcgattatt taccggatta atttcaaaca tggtctgatt tcttttattg agctagtcaa
     5161 aatgcggtga taagagcggg attgtaccca atccacgctc ttttttatag agaagatgac
     5221 gctaaattgg ccagatattg tcgatgataa tttgcaggct gcggttgccg cgaaactcgt
     5281 tgatatcgag cttataagcc agttgcactt cgcggacgcc gttatccggc cagagagcgg
     5341 tatcgacatt aaaagcaata ccatccagca gtggaccgcc gccgaccggt tcgaccatca
     5401 ccttcaaatg acgttcgccc accagccgct gttgcagcag acggaaatga ccgtcaaaca
     5461 gcggctccgg gaacatctgc ccccacgggc cagcatcgcg cagcagctgc gccacttcca
     5521 tggtcatttc ggccgggctt aacggaccgt ctgacaccac ttcgccttgc aatagcgaag
     5581 gggccagcca ctcagtaacc agttcgccaa accgttgttg aaagagttcg aatttatcct
     5641 cttccagcga caaacccgcc gccatcgcat gaccgccaaa cttgagcatc atgccggggt
     5701 agagtgtgtc taatcgctcc agcgcatcac gcatatgcag cccctgaatg gagcgaccgg
     5761 aacctttcag cgtaccgtca cctgctggcg caaaggcgat aaccggacgg tgaaaacgct
     5821 ctttgatacg cgaagccaga ataccgacaa cgccctgatg ccattcgggg tgatacattg
     5881 ccagcccgcc gggtagcgtg tcacggctgc gctccagttt ctcgcacagg gtcagggctt
     5941 caacctgcat tccttgttcg atctcttttc gcgtctggtt tagcgcatcg agttcatttg
     6001 ccagcacgcg cgcttcgccg atgttgtcgc acagcaacag cgccacacca acggacatat
     6061 catccagtcg cccggcagca ttgagacgcg gtcccagcgc aaaacctaaa tcgctggcgg
     6121 ctagtttttg tgcatcgcga tttgccactt ccagcagcgc tttaatccct ggacggcact
     6181 ttccggcacg gatgcgactc atcccctgcc aggtcagaat gcgattatta gcgtccagcg
     6241 gcacgacgtc cgctactgtc cccagcgcta ccagatccag cagttctgcc aggttaggaa
     6301 ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa aaaggtgcgc agcgccagca
     6361 tcagataaaa cgccacaccc acgcctgcca gtgatttcga cgggaaatta cagtcgcgca
     6421 agttagggtt aatgatcgct tccgctgcgg gtaatgtgtc gcccggcaaa tggtgatcgg
     6481 taacaataac cgggatgccc aacgagcgag cgtgctcaac tcccgcatgg gaggaaatac
     6541 cgttatccac cgtgacaatt aactgcgcgc cacgggcatg ggcctgatcg accacttccg
     6601 ggcttaagcc gtaaccgtct tcgaaacggt ttggtaccag gtagtcgata ttgctgcaac
     6661 caagcgagcg catcgccagc acgcttagag ccgtgctggt cgcgccgtcg gcgtcgaaat
     6721 caccgaccac aataatccgc gttccttcgc gaaaagcgtt gtaaaggatc tcaacggcct
     6781 tttcgacacc gcttagttgc tgccagggca acataccttt aacactgcgt tccagttctt
     6841 gcgcactgcg tactccccgg ctggcgtata aacggcgcag caagggaggc aattcagcgg
     6901 gcaagtctgc cgtttcatcg acttcacggc gacgaagttg tatctgttgt ttcacgcgaa
     6961 ttatttaccg ctggtcattt tttggtgttc gtcgaggaat tctttcatgt ctttcggcgg
     7021 ctggtaaccc ggaacaagtg tgccattgct cagcacaact gccggagtac cgctaacgcc
     7081 aagctggacg ccaagtgcgt aatggtcggc aatatccacg tcgcagctgg ctggtgcgac
     7141 gcttttacct gccatcacat catcaaacgc tttgttttta tctttcgcac accagatagc
     7201 tttcatttct ttctctgcat cgctgtccag cccctggcgc gggaaagcaa gataacgcac
     7261 ggtgatcccc agcgcgttat agtctgccat ttgctcatgc agtttgtggc agtaaccaca
     7321 ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc ggcgctttat aaacgatcat
     7381 ctctttttca agcgcattca actgctttaa cagcatctta ttggtgacat tgaccggagc
     7441 cgtgccacta acgtcataca ttggcccctg aatgatatgt ttaccatcat cggtgatgta
     7501 caacacaccg ctgttagtca gaactgtctt catgccagct acgggcgcgg gctgaatatc
     7561 gctgcttttg atgcccattt tggctaacgt ttgttgaatt gccgcgtcat cagcctgagc
     7621 aaagcctgaa aacgccgcta acaaagtaaa caacataaaa cctttcttca taaatcttcc
     7681 cgttcttttc agacatcacg cccgcgggtg atgctgttga tgaagttgcc gcagacgctc
     7741 ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg ctgtggccca gtagcatctg
     7801 caccacgcgt aaatccgcac catgatttaa taaatgagtg gcaaaagcgt gacgcaacac
     7861 atgcggtgac agcttttcgc tgtcgatacc cgccagcacg gcataatgtt taatgcggtg
     7921 ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg ggaaacaaca cgtcaattga
     7981 cacaccattc agcagccacg gacgcccatg ttccagatag gtttccagcc agtaaaccgc
     8041 ctcttcccct aacggcacca ggcgctcttt gttgccttta ccaatgaccc gcactacgcc
     8101 ctgacgcagg ctgatatcac tcattgtcag cccgaccagt tcagagacac gcagcccggt
     8161 agcatacaac acttcaagca tggctttatc gcgtagctcc agtggctgat caattaatgg
     8221 cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct tttggcaaac gctggggcaa
     8281 tttcggtgaa gcgagatgcg cactgggatc gtcttcacga aacttttcgc gataaagata
     8341 ctggaacaat cggcgcactg cactcagcaa acgcgctgag ctggtggctt tatacccgcc
     8401 ctccagccgt tctgccagta atgcctgcaa atcgtcactt tgcgccgtcg ccagcgtcaa
     8461 cccgcggtga tgcaaccact ccaccatcat tgacagatcg cgacggtaag cgttcaacgt
     8521 attttcagcc agattttttt ccagccacag agcatcaaga aactgctcga tgcgtgccag
     8581 atcctgtttc acttgcgccc cttatggtca ctcatttgat ccattatgcc ttattgtgcc
     8641 gtgactaaag cgattctgat acactagctg caaaagccac agcagaatcg agaagcttac
     8701 gttatgaata tgggtctttt ttacggttcc agcacctgtt acaccgaaat ggcggcagaa
     8761 aaaatccgcg atattatcgg cccagaactg gtgaccttac ataacctcaa ggacgactcc
     8821 ccgaaattaa tggagcagta cgatgtgctc attctgggta tcccgacctg ggattttggt
     8881 gaaatccaag aagactggga agccgtctgg gatcagctcg acgacctgaa ccttgaaggt
     8941 aaaattattg cgctgtatgg gcttggcgat caactgggat acggcgagtg gttcctcgat
     9001 gcgctcggta tgctgcatga caaactctcg accaaaggcg tgaagttcgt cggctactgg
     9061 ccaacggaag gatatgaatt taccagcccg aaaccggtga ttgctgacgg gcaactgttc
     9121 gtgggtctgg cgctggatga aactaaccag tatgacctta gcgacgagcg tattcagagc
     9181 tggtgcgagc aaatcctcaa cgaaatggca gagcattacg cctgatctca ctgacggctt
     9241 agcgcatatg ctttgccgtc atctttatct ttgcgtctct tgttgcaaca aaatccgccg
     9301 taaatcccgc cattcggctt cgtccatgct gtcggctgcc agccataaat gttgccgttt
     9361 accgccatca gaacgtaaac gcagcatcat gccgctctta atcatccacg gtgctttgac
     9421 gatgctccac tcctgccctt gccaacgcaa acgcccgtcc atcaacaagc gaatttcccc
     9481 ctggcgagca ttaatacacc gctggctgcg aacgcaatca aataccacca gcgaaagtaa
     9541 caccatccat aacggggtgt aactgagcgg ccagggcatg agtaaaataa cagcggcaac
     9601 cagcccatga atcagcaagg aaagccactg tgcgcgccag gagacgcgca aatcagattg
     9661 ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg gaccattatt tccagttctg
     9721 catcggccgg tttaccgtga ttcatcagcc agttaaacag gtccggatcg tcacattcca
     9781 gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct gtcgtactca tgttcgaaaa
     9841 acggcatgat tgaaatatcg agttcgcgca taccacggcg gcatgcccaa tgaatgcggg
     9901 ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa tccagtaccc ggctattgta
     9961 acgtgttttt cgacttcttt tacgggaata tcagtaaaca ccatcgcgat cgcgaaatta
    10021 attcacaaca attcaagggc ttcatttttt tggcagtcgc ctcgcagaag gcacagatcg
    10081 cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc attagtcatt aatacgccgt
    10141 taagcaactc aggactctat tatggctttt acaccttttc ctccacgtca gcctacggct
    10201 tctgcccgtt taccgctgac gctgatgacg cttgatgact gggcgcttgc caccattact
    10261 ggcgcggaca gcgaaaaata tatgcagggt caggtgacag cagatgtcag ccagatggca
    10321 gaagatcagc acctgctcgc cgcccattgc gacgccaaag gtaaaatgtg gagcaattta
    10381 cgtctgttcc gcgacggcga tggctttgca tggattgaac ggcgcagcgt gcgtgaaccg
    10441 cagctgactg aactgaaaaa atatgcggta ttctctaaag tgaccatcgc gccagacgac
    10501 gagcgtgtgc tgcttggtgt tgccggtttt caggcgcgcg ccgcgctggc aaatctcttt
    10561 agcgaactgc cttcgaaaga aaaacaggta gtcaaagaag gcgcgaccac tttgctatgg
    10621 tttgaacacc cggcagaacg tttcctgatc gtaaccgatg aagctactgc taatatgctg
    10681 accgataaac tgcgcggtga agcggaactg aacaatagcc aacagtggct tgcattaaac
    10741 attgaagctg gtttcccggt gattgatgcc gccaacagcg ggcagtttat cccacaggcg
    10801 accaatctcc aggcgctggg cggtatcagc tttaagaaag gctgttatac cggacaagag
    10861 atggtggcgc gagcaaaatt ccgtggtgcc aacaaacgcg cgctctggtt gctggcaggt
    10921 agtgccagcc gactgccgga agctggtgaa gacttagagc tgaaaatggg cgagaactgg
    10981 cgtcgtaccg gtacggtgct ggctgcggta aaactggaag atggtcaggt cgtggtacag
    11041 gtcgtcatga ataacgatat ggaaccggac agcatcttcc gcgtacgcga cgatgcgaat
    11101 acactgcata tcgagccgct gccgtattcg ctcgaagagt aaatctctgt atcgcatcag
    11161 gcatttatcg cctgatgcga cgctgacgcg tcttatcatg tctgggattt gatgccttta
    11221 tcacaacagg gacgaggccc gtagatcgga taaggcgttc acgccgcatc cggcggttgc
    11281 gctccgatgc ctgatgcgac gctgacgcgt cttatcaggc ctacaaaggc atacccatta
    11341 cgcctgccca atatacaaat agatcgccag aaagtggcac acactaccgc cgagcacgaa
    11401 gccgtgccag atggcatggt tgtatggaat gcgtttgcag acgtagaaaa tcaccccgag
    11461 cgaataaacc acgccgccta ccgccagtaa ggtaacgctg cccgccgcga gcttaactgc
    11521 catttcataa attaccacca gcgacagcca gcccatcgcc agataagtca ccagagataa
    11581 aattttgaat cggtgcgcga tggtcagttt gaacagaatc cccagcaatg ctaggctcca
    11641 gataacaatc atcaacccgc gcgctaacgg agaatccagc cccaccagca aaaacggcgt
    11701 gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca aatttcttca gccacatttt
    11761 tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg aggaacagca ggatcatact
    11821 gccgccatag aggctgtaac tggttatcgc tgtggcgctg gcattgagat ccaccgcctg
    11881 aaccagtagc aacaccagcc caacgatacc aaacaccagc ccaatgccgt gactgacgct
    11941 gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg aggggcttct gaaccataac
    12001 ttactccaga gaaacgtaca cgcacatgta tacctctcca gcgtaactga gaatagttcc
    12061 agtgaacacc tgttagctaa aataaatttt gatttgagac taatctccta aaaatcatga
    12121 aattaaatgc gaaatttcaa ctaacaggcg tcagttcaat ttaaagacat ttaaattcaa
    12181 tcatataaaa ttgtgtctga ccgggataga tgtcagcaat gacctttttc agttcaggca
    12241 gggtcatatt ttcctgctct gcatgttttt ccgtcagtgt atccagcgtt acggttgagg
    12301 ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc aaaacgtccg acacgaagca
    12361 catcacccgt tttgaagtgc gattcagact cgtcgcggat ggtgatggtt ttacgcccag
    12421 ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc gtttggctgc atggtactat
    12481 ttcctgtaag aattgactga tctggagcct atgatagtga aaaaactcac cttaccgaaa
    12541 gatttcttat ggggtggcgc cgttgccgct catcaggtcg aaggcggctg gaacaaaggc
    12601 ggcaaaggac cgagcatttg tgacgttctg accggtggcg cacacggcgt gccgcgcgaa
    12661 atcaccaaag aagtcttgcc aggaaaatac tatccaaacc atgaagccgt tgatttttat
    12721 ggtcactaca aggaagacat caagctattt gccgaaatgg gcttcaaatg ttttcggact
    12781 tccatcgcct ggacgcgtat ttttccaaaa ggtgatgaag ctcagccaaa cgaagaaggg
    12841 ctgaagtttt acgactctct gttcgatgaa ctgctgaaat acaacatcga accggtgata
    12901 accctctccc actttgaaat gccgctgcat ctggtgcagc aatacggtag ctggaccaac
    12961 cgtaaattcg ttgatttctt tgtccgtttc gcggaagtgg tattcgaacg ctacaagcat
    13021 aaagtcaaat actggatgac cttcaacgaa ataaacaacc agcgtaactg gcgtgcgccg
    13081 ctgtttggtt actgctgctc cggcgtggtg tataccgagc atgacaaccc ggaagagacg
    13141 atgtaccagg tgctgcatca ccagtttgtc gccagcgccc tggcagtgaa agccgcgcgt
    13201 cgcattaacc cggagatgaa agtcggctgt atgctggcga tggtgccgct ctatccctac
    13261 tcatgtaacc cggacgatgt gatgttcgct caggagtcga tgcgcgaacg ctacgtcttt
    13321 accgatgtgc agctatgcgg ctattacccg tcctatgtat tgaacgagtg ggaacgtcgc
    13381 ggatttaata tcaaaatgga agacggcgat ctcgatgtac tgcgcgaagg cacctgcgat
    13441 tatcttggtt tcagctatta catgaccaac gcagtgaagg ccgaaggcgg caccggcgat
    13501 gcgatctctg gttttgaagg cagcgtaccg aacccgtatg ttaaagcgtc tgactggggc
    13561 tgcagattga tccagtaggt ctgcgctatg ccctttgcga actgtatgag cgttatcaga
    13621 ggccgctgtt tattgtcgaa aacggttttg gcgcttacga caaagtggaa gaagatggca
    13681 gcatcaacga cgactaccgc attgactacc tgcgcgccca tattgaagag atgaaaaaag
    13741 cagtgactta cgatggtgtg gatctgatgg gctacacacc gtggggctgc atcgactgcg
    13801 tgtcattcac taccgggcag tacagcaaac gctacggctt tatctatgtg aataaacatg
    13861 acgacggtac tggcgatatg tcgcgttcac gtaagaagag ctttaactgg tacaaagagg
    13921 tgattgccag caacggcgag aagctttaag tcgatgaagt accggatgca atacttgttg
    13981 catccggtca gacaacttcc ctgttttatt tcccgcccgc caaatcgata aaacttcccg
    14041 tgacgtaaga ggctttatca cttagtagcc agacaatggc ctgcgcgacc tcttctgcct
    14101 gtccaccacg ctgcatgggg atgttcgact taacgcgatc gacgcgtcca ggctcgccgc
    14161 cgctggcgtg catttcggta taaataaacc ctggccgcac gcagttaacg cggatcccct
    14221 gcgcggcgac ttccagcgat cgtccggtgg ttaacgtatc aatcgcccct ttcgatgccg
    14281 cgtaatcaac atattcccct ggcgaaccca accgtgaggc caccgaagag acattgacga
    14341 tagcgccgcc actgccacca tttttaagcg ccatgcgttt taccgcctcg cggcagcaga
    14401 gaaaatatcc cgtcacgttg gtggaaagta ctcggttgat tcgctctgcg gtaaggtttt
    14461 caacggtgca ctgggtaaac aagatcccgg cgttattgac cagcgctgct agcggttcat
    14521 cgtgctgatc gattgctgta aacatcgcaa cgacctggtt ttcgtcgctg atatccgcct
    14581 ggagcacgaa tgctttgcca ccggcttgca ttattaagtt catcacttcc tgcgccgcgt
    14641 ggaggttttg ctgataatta accgccaccg tatacccttc ttgcgccaac agtaatgcag
    14701 ttgcccgccc gatgccgcga ctgccaccgg tcacaagtgc tatagccatt tgtttttccg
    14761 agaaaaatta attcgttgac gtatctttga gattgctaac aaactggctc aatcttcaat
    14821 gttggcacgc ttttccccct cgccctttca gggagatggt cggggtgagg gtaaatattc
    14881 gcgccagtgc cggcctgttc ccctcaccct aaccctctcc ccaaaggggc gaggggactg
    14941 tctgagcact tttgtacttt gtcatctgac taaaaaggcg ccgaagcgcc tttagaaaat
    15001 actcgaatca gtgaattact ggtattcgct aatcggtacg caggagcaga acaggttacg
    15061 gtcgccgtaa acatcatcca gacgtttcac cgtcggccag tatttgtctg ccacgcctgc
    15121 cgggaatacc gcaacttcac ggctgtacgg atgcgcccac tcggcgacca gttcgctctg
    15181 aatgtgcggc gcgttcacca gcgggttatc ttccagcggc cagacaccgg ctttcacctg
    15241 gtcaatttct gcgcggatag ccagcatcgc gtcgataaag cgatccagtt ccactttgct
    15301 ttcagattca gtcggttcaa ccatcagcgt acccgccacc gggaacgaca tcgtcggcgc
    15361 gtggaaaccg tagtcgatca ggcgcttggc aatatccagc tcgctgatgc cgatttcttc
    15421 tttcagcggg cgaatatcga gaatacattc gtgcgccacg cgaccgtcgc gaccggtata
    15481 cagcaccggg aaggcatcct gcaggcggct ggcaatatag ttggcgttga ggattgccac
    15541 ctggcttgct tttttcagcc cttctgcgcc catcatgcgg atgtacatcc agctgattgg
    15601 caggatagag gcgctaccga acggtgccgc agaaactgcg ccctgacggg ttaacatgcc
    15661 ttcgatttgc accacgctat gacccggtac aaacggtgcc agatgcgctt tcacaccgat
    15721 cggtcccata cccggaccac caccgccgtg cggaatgcag aaagttttat gcaggttgag
    15781 gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg ccaacctggg cgttcatgtt
    15841 cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg acttcacaca cttcacggat
    15901 cgtttcttca tacacgccgt gggtagaagg ataagtcacc atgatacagg agaggttatc
    15961 gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg atgttgccgt ttttatcaca
    16021 cgccacaacc accacctgca ttcctgccat atgtgcagaa gcggggttag ttccgtgcgc
    16081 agaagccggg atcaggcaga tatcgcgatg cccttcgttg cggctttcat gataatgacg
    16141 aatcgccagc aggcccgcgt attcgccctg tgcgccggag ttcggctgca tacaaacggc
    16201 gtcgtaaccg gtcagtttca ccagccagtc agccagctgc gcaatcatct gctgataacc
    16261 ttcagcctgt tccggcgggc aaaacgggtg cagttcggca aattccgtcc aagtgattgg
    16321 gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa cccagcggga tcatcgcctg
    16381 attcagcgcc agatctttac gctccagcga gtgcatatag cgcatcattt cggtttcgct
    16441 gtgatagcga ttaaacaccg gatgggtgag gatttcgtcg tcgcgcagca tcgcaggctg
    16501 gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg tcgatctcca gcccgtggtt
    16561 atcgcccagc agcacactga aaagctgcat tacgttttca cgcgtggtgg tttcatcaag
    16621 ggtgatcccc acggcgttca ggatatcgct acgcaggttg atttcagccg cttcggcacg
    16681 cgtcagtacg cccgctttgt cggctacttc cacacacaag gtgtcgaaat agtgcgcatg
    16741 gcgcagtttc agaccttttt gttgcaggcc cgccgccaga atatcggtca gacggtgaat
    16801 gcggttagcg atacgtttca ggccaaccgg gccgtgataa acggcataca ggctggcgat
    16861 gtttgccagc agtacctggg aagtacaaat gttggagttc gctttctcac gacgaatatg
    16921 ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg ccagctgcat ctttcgatac
    16981 accgataata cggcccggca ttgagcgttt gtattcatct ttcgccgcaa agaatgccgc
    17041 gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc gccgaaccaa aaacaatatc
    17101 cgcgccctgt ttacccggcg cagttaacag caccagcgcc ataatatcgg cggcaacgct
    17161 gaccacaatt ttgcgtgatt tcagttcgct aataagcgca gtgtagtcgt gaatttcacc
    17221 ggtagtgcct acctgctgta acagcacgcc gaagacgtcc tgatggtcga gcactttttg
    17281 cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca cgagtacgga ccacatccag
    17341 tgtttgcgga tgcacattgg aagccacgaa gaagcggttg gcatttttca gtttgctgac
    17401 gcgtttcgcc atcgccattg cttcggcggc agcggtggcc tcgtccagaa gagaagcaga
    17461 ggccatatcc agtccagtca aatccagcgt tacctgctgg aagttgagca gtgcttcaag
    17521 gcggccctgg gagacttcag gttgatacgg agtgtacgcg gtataccagc ccggattttc
    17581 cagcatgtta cgcaggataa ccggcggtag ctgcacggcg gtgtaaccca tgccgatgta
    17641 agacgtgaag cgtttattgc gactggcaat agccttgagt tctgccagtg cggcgtattc
    17701 ggtcgccggt gcgccaacct gcggtggtgt cgcaagctga atatctttcg gcacaatctg
    17761 gccggtcagc gcgtttaacg attgtgcacc aacggcattc agcatttctt gctgttgcgc
    17821 ggcgtccggt ccgatatggc gttcaataaa agcgccgctg ttttcaagct ggcttaacgt
    17881 ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt gtgaacctct ctccttacga
    17941 agagagtgag ggtgaggcat aaattttcct caccctgatc ctctcccgca gaagaggaat
    18001 aaagccgtta ctcatcttct aacaatgctt cgtatgcggt cgcatccagc agtgattcca
    18061 gttcgctttc atcgctagct ttgattttaa agatccagcc gcctgcatac ggttcgctgt
    18121 tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc cacgatttca ccgcttactg
    18181 gcgcataaat gtctgacgcc gcttttaccg attcggcaac cgcgcagtca tcgcccgcgc
    18241 taaccgttgc gcccacttcc ggcaggtcaa caaacaccat atcgcctaac agctcctgag
    18301 catgttcggt aataccaacg gtgtaagtgc cgtcggcttc tttacgcagc cattcgtgtt
    18361 ctttgctgta tttcagttct gctggtacgt tgctcatcaa tcaatctcca aaaaagtaaa
    18421 tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt gtcactttaa ccggcatttc
    18481 acggttgcga atttgcacaa tcgccgtttc gccaatacct tccggcacgc gcgccagcgc
    18541 aatgctgtaa cccagcgtcg gggagaaagt accgctggtg ataatgcctt catgctggtt
    18601 gccctgcgca tcggtaaagc gtaccggcag ttcattacgc agcacgcctt tttcggtcat
    18661 caccagacca accagttttt ctgtaccatg ctcacgctgc acttccaggg cttcacgacc
    18721 gataaagtca cgatctgtcg gttcccaggc gatagtccag cccatgttgg cggctaaagg
    18781 agaaatagtt tcgtccatct cctgaccata aagattcatg cccgcttcca gacgcagcgt
    18841 gtcacgcgcc cccagaccgc agggcttaac gcccgcttcc accagcgcac gccagaaatc
    18901 ggccgctttt tcattgggca gcgcaatttc atagcccgct tcaccggtat aaccagtggt
    18961 ggcaataaac agatcgccca cctgcacgcc aaagaacggt ttcatccctt ccaccgcctg
    19021 acgctgagca tcattaaaca gtgtggcagc ttttgcctga gcattcggcc cttgcacggc
    19081 aatcatggaa aggtcatcac gaacggtaat ttcgatgccg aaaggttcag cgtgttgggt
    19141 aatccaggag aggtcttttt cgcgggtggc ggagttaaca atgaggcgga agaaatcttc
    19201 agtaaagtag tagacgatga gatcatctat cacaccgcca gaggcattca acatccccga
    19261 gtaaagggct ttgccgcttt tggtgagctt cgccacatcg ttcgccagca gataacgcag
    19321 aaactcccgg gtgcggctgc cgcggagatc gacgatggtc atatgcgaca catcaaacat
    19381 tccggcatcg gtacgtaccg catgatgttc gtcgatttgc gaaccgtaat gcagcggcat
    19441 catccagccg tggaaatcca ccatgcgagc gccgcaaagc gtgtgttgtt cgtacaaagg
    19501 agtctgttgt gccatcttgt cctcattgaa taagcggggc tgacaacttt ttcatggtga
    19561 aattatcacc acaaaaccca gcatcggagc cactcccggt ccccaacgca atcgttctct
    19621 tttgcctgaa cttaccaccg aaacagactg ttaaccataa ggtaaaattg atcatcacat
    19681 tagcttatgg ttaaaaaatg caaaaatcgc gacagaataa aaaaccaaaa aatacaccaa
    19741 tttctataca aagatgatgt gatgagaaag tcaatttgaa taagacaata ttaagagcta
    19801 aaaaaatgtc aaaaaacact aaatcaaaaa atcatggcat tagaaaatat aatgcgaaaa
    19861 cggaggtgaa attagtttat ttcaaatgag gaaaatctcc cggcgaaaaa accgggagat
    19921 gaaagtgtga tgggtatcaa ataaacaaca gaggagaaat ttttaacgca gccattcaga
    19981 caaatcgttt aatcccattg cctggcggat aagttgcggc ttaacgccag gaagcgtgtc
    20041 ggccagtttc aaaccaatat cacgcagcag ttttttcgcc ggattagcgc cggaaaagag
    20101 atcgcgaaaa ccctgcatac cagccagcat cagcgccgcg ctgtgcttgc ggctacgttc
    20161 atagcggcgc aggtaaatgt actgcccgat gtctttaccc tgacgatgca accgtttcag
    20221 ttcagcaatc agctctgcgg cgtccataaa tccgagatta acgccctgcc ccgccagtgg
    20281 gtgaatagta tgcgcggcgt cgcccaccag cgccagacgg tgtgcggcaa actggcgcgc
    20341 ataacgcccc gtcagcggga acacctgacg cgcgctctca accttgcata agccaaggcg
    20401 attatcaaaa gcgatattta acgcgcgatt aaattcgtct tcacttgcct gctgcatccg
    20461 ctgcgcttcc tctggcgaca gtgaccagac aatcgagcaa agatgcggat cgctaagcgg
    20521 taaaaaggcc agaatgcctt cgccatggaa aacctgccgc gccaccgcat catgcggttc
    20581 ttccgtgcga atggtcgcta ccagcgcgtg atgctgataa tcccagaaag tcagcggaat
    20641 atctgctttg ttgcgcaacc aggaattagc accgtccgcg ccaatcacca gacgcgccgt
    20701 taacatgctg ccatctttca gcgtcaggaa ggtttcattt tctccccagg cgacctgctg
    20761 taattctgtg ggggccaaca gagtaatatc tgacgactgc tgcgctttgt tccacagcgc
    20821 gtagtgaatc actgagtttt caacgatgtg cccaagatgg ctatagccca tgctttgatc
    20881 gtcaaacgag atgtgaccaa aactgtcttt gtcccacact tccatgccgt gatagcaact
    20941 ggccctacga gcgagaatgt cctgccagac gccaagacgg gtgagtaatt tttcgctggc
    21001 ggcattgata gccgaaacgc gcagttgtgg tagtgcatct accgccagag gttccggtac
    21061 gcgctgctcc agtacggcaa cgcgtaagcc gctcccctgt aagccgcagg caaccgccag
    21121 ccccaccatg ccgccgccaa caatggctac atcaacactt tgcattgttt attccttaaa
    21181 accgccttca acgcgccacc caaccgaggg tgcgctgcgc cagcacatcg cgtgccgggg
    21241 tgaataattc catcgtcatc agtccgatgt tgcgcccgac aaccagcggt gcccaacggt
    21301 tggcaaaaag atgtaccagg ctgtccgtga cgccgatggt tgcttcgcga tcgctctgtc
    21361 gacgctgctg atagcggcac aatatgccgt aatcaccgat gtcttctccg cgctcatgcg
    21421 cctgagtcag ggtttccgca aggctcatga catctcgcat accgaggtta aacccttgcc
    21481 cggcaatcgg gtgcagagtt tgcgccgcat tgcccaccag cacagtgcga tgggtaatcg
    21541 gtctggcggc gcgggttaat gccagcggat aagcactgcg tttaccggcg tgggtaattt
    21601 gccccagtcg ccagccaaag gcagactgga gttcacggca aaacttctcg tcgctccacg
    21661 acagcacctc ttcgcgccgt tccagtggat gacaccagac cagcgaacag cgtccgtcag
    21721 acatcggcaa catcgccagc gggccatgtt gcgtgaagcg ttcaaaagcg cggccttcgt
    21781 gcgcaacaga agtagcaacg ttggcaatca ctgccagttg ttcgtaaggc tcctgctgcc
    21841 aatcaacgcc gcacgcggtg gctaacgctg aatgggtgcc atcagcggct atcagcacgc
    21901 ggcccgtcag catctcgccg ctctccagcg tcacttcaac gtgactctga gtacgggcaa
    21961 cgttagctac gcgatcgggg caatgcagca ttacgccagg tgcttttcgc agcaatgcaa
    22021 acagccgttg cccaacatta tgcaattcga caacctgccc cagcgccgcc agttggtaat
    22081 cttctgcggc gagggtgacg aatccagcat gaccacgatc gctgacatgc acggtagtga
    22141 tggcggttgc gcaatcagcc agagattgcc agacgccgat gcgcgccagt tgctgacagg
    22201 tacccgccgc cagcgctatc gctcgtccat caaagcccgg atgagcatgt gactctggcg
    22261 cagtcgcttc aatcaaatgt accggcagcg ccccgtgact taaccgggaa atagccagcg
    22321 ccagcgtcgc gcccgccatg ccgccaccga cgatgattac gctcattgct ttctcgcagc
    22381 agccatcaac gcttcgattt cttccggctt tttcaccacg ctggcggtga ggttttcgtt
    22441 gccggtttcg gtaatcacaa tgtcgtcttc aatacgaatg ccgataccgc gatattgttc
    22501 tggcacatcc gcatccggcg cgatatacag accaggctct acggtcagta ccatccccgg
    22561 ttccagaatg cgcgagcgat cctgaccata aacgccaacg tcatggacat ccagtcctaa
    22621 ccagtggcta aggccatgca taaagaaagg acgatgggcg ttctgagcga tcagttcatc
    22681 aacattacct ttcaggatgc cgagttttac caggccgcta accatgatgc gcaccacttc
    22741 accagtgact tccagaatgg aagttcccgg acgatacagg cgcaggctgg tttcgagaga
    22801 ctccagcaca atgtcgtaga tttcacgctg ggcctgggtg aatttgccgt tgaccgggaa
    22861 ggtgcgggta atatcgcccg cgtaaccttt gtattcacaa cccgcgtcaa tcaacaccag
    22921 gtcaccgtcg cgcagttcac tctcgttttc ggtgtagtgc agaatgcagc cgttttcacc
    22981 gctgcctaca atggtgttat aggaaggata gcgcgcaccg tggcggttaa attcgtggtg
    23041 aatttcgcct tccagatggt actcgaacat tcccggacgg catttttcca tcgcccgtgt
    23101 atgtgccatg gcggtgattt ctcccgcgcg gcggagtacg gcaatctctt ctggcgattt
    23161 gaacaggcgc atttcatgaa caacaggacg ccagtcgatc atcgttgccg gtgcggtgag
    23221 attttgccgc gaacctttac gcagtttttc cagcgcactg ttcacgattt catcagcata
    23281 tgcatattcg ccctgggcat ggtaaaccac atccaggccg ttaagtagtt gataaagttg
    23341 ctgattgatt tcgctgaatg ccagtgcgcg gtcaacgccc agtttctctg gcgcggcatc
    23401 ctggcctaag cgacggccaa accagatctc cgccgtcagg tcgcgaacgc ggttaaacag
    23461 aacgctgtgg ttatgagtgt catcgctttt aatcagcacc agcaccgctt ccggttcgtt
    23521 aaagccggtg aagtaccaga agtcactgtt ctgacgatag gggtattcgc tgtcggcgct
    23581 acgtgttact tctggtgcag caaaaatcag cgcggcgctg ccgggttgca tttgctccac
    23641 cagggcctga cggcgacgct gaaactcttg ccgggatatc tcactcataa cactctcctt
    23701 acgttttttg tttttagtgt agagtcggtt tttgtacttc tggcgcggtc ggttgcggat
    23761 gagtaaaggt gtcgtggcat aacagcgcgg caacacgaac gtattcgatg atctcttcaa
    23821 gcgacatttc aagctcttcc tgatcttcgt cttcgtcgta gcccagctgc gcgatgttac
    23881 gcagatcgtc gatggcttca ccggtttcgc cggtcacttt gtccagcttc ggttgcgtaa
    23941 cgccaagacc aagcaggaag tgattgaccc aacctgccag tgcatcagcc cgatcgaaaa
    24001 cgctgacatc atcgccatca ggcagataaa gctgaaaaag gaagccgtca tcctgcaggg
    24061 catcgctggt ggcagagtgc attttacgca gtgcctgtgc cagctcatga ccgaaagcca
    24121 tgccttcgtt cgtcaggtcg tgaagtagcg gcagccatga gctgtcatcg ttaccgccac
    24181 atatcatccc actgattaaa ccatgcatct cggcaggggt cagacccgtc ccttgttggt
    24241 tcagatactg gttcatttcg ttgtaaccag gcatttcgtt ctgtatagac ataagcattc
    24301 gtcgtcaaag ggaggaatat tcatgatatg ctaccacttt gggccctggt ggaccagaaa
    24361 agggcttgtc tcttctcatc agggtagcta tagcgtcgcc ccttcgcaga ccatgggtct
    24421 aaagacgaag gcagcgcagt caatcagcag gaaggtggca tgtctgcaca acccgtcgat
    24481 atccaaattt ttggccgttc actgcgtgtg aactgcccgc ctgaccaaag ggatgcgttg
    24541 aatcaggcag cggacgatct gaaccaacgg ttgcaagatc tgaaagaacg cactagagtc
    24601 acaaatactg aacagttggt cttcattgcc gcattgaata tcagctatga gttagcgcaa
    24661 gaaaaagcaa agactcgtga ctacgcggca agtatggaac agcgtattcg gatgctgcag
    24721 cagaccatag aacaagcgtt acttgaacaa ggtcgcatca ccgaaaaaac taaccaaaac
    24781 tttgaatgac acttttcggt ttactgtggt agagtaaccg tgaagacaaa atttctctga
    24841 gatgttcgca agcgggccag tcccctgagc cgatatttca taccacaaga atgtggcgct
    24901 ccgcggttgg tgagcatgct cggttcgtcc gagaagcctt aaaactgcga cgacacattc
    24961 accttgaacc aagggttcaa gggttacagc ctgcggcggc atctcggaga ttcccttctt
    25021 atctggcacc agccatgacg caactaccag agctcccact gacattatcc cgacaagaaa
    25081 tccgcaaaat gattcggcaa cgtcgtcgtg cgttaacgcc ggaacaacag ctggaaatgg
    25141 gtcaacaagc cgctacccgg atgatgactt atcccccggt ggtgatggcg catacggtcg
    25201 ctgtattcct ctcttttgat ggtgaactcg acacccagcc gctaatagaa caactctggc
    25261 gcgccggtaa gcgcgtatat cttccagttt tgcatccctt tagtgccggt aatttgctgt
    25321 tcctgaatta ccatccgcaa agcgaactgg tgatgaacag gttgaagatc catgagccaa
    25381 aactggatgt gcgtgacgtg ctaccccttt cccgattaga cgtgctgatc acaccgctgg
    25441 tcgcctttga tgagtacggt cagcgcctgg gaatgggcgg tggtttttat gatcggacct
    25501 tacaaaactg gcagcactat aaaacgcaac cggtgggtta tgcgcatgat tgtcagttgg
    25561 tggaaaaact ccccgttgaa gagtgggata tccctcttcc tgcggtggtt acaccgtcga
    25621 aagtctggga gtggtaaggg cgatacacct gcatcgccct gattgacatc gttgattctt
    25681 taacctaatt tagtgagtaa gggtaaggga ggattgctcc tcccctgtga ctgttaataa
    25741 gctgggaaac ttatgagtaa cagtacaatc agtatgatgg caagtcgcat cataaccctt
    25801 ctccttcaag ccctcgcttc ggtgagggct ttaccgttac agccccatgc tgcccagcta
    25861 tcgtaagctc tctttaaata accacaacgc attgatctac ctttaaatct tttcttcgag
    25921 cgtgctcgaa aaaccccatg taaaacagag ataatttttg ggaaaaagga tctattacgg
    25981 cagtaaacgt gggcgatgaa gtggctatca aaaagttcgg ttcagggcga tgcaccccgc
    26041 atcgccctga ttgacatcgt tgattctttg acctaattta gtgagtaagg gtaagggagg
    26101 atgactcctc ccttgagact gactgttaat aagctccgaa acttatcagt aacagtacaa
    26161 tcagtatgat gacaagtcgc atcataaccc ttctccttta agccctcgct tcggtgaggg
    26221 ctttaccgtt acagccccat gctgtcccgc catcgtaaat ccccattaaa taaacacaac
    26281 gcattgatct gactttgatt tattttctgg agcagactcg caaagtagaa tgcgcaacgc
    26341 ggcaacggtg tggagaaggg ataaaaaaac gggcaagtca gtgacctgcc cgttaatttt
    26401 cagagaaggg gaattagtac agcagacggg cgcgaatggt acccggaata gctttcattg
    26461 cctgcagcgc tttttcggca acgtcttcgt cagcttcaat atcaataact acataaccca
    26521 tctgggcgga agtttgtaga tattgcgcgg cgatgttgac gccctgctcg gcgaagattt
    26581 tgttcagcgc agttagcacg cccggacggt tttcgtggat gtgcatcaga cgacgaccac
    26641 catgcagtgg cagcgagact tccgggaagt tcaccgcaga gagcgttgag ccattgtcag
    26701 aatacttgat caatttaccc gcaacttcca agccgatatt ctcctgcgct tcctgagtcg
    26761 aaccgccaat gtgtggcgtc agaaggacgt tgtcgaattc acacagtgga gaggtaaatg
    26821 gatcgctatt ggtcgccggt tccgtcggga atacgtcgat tgccgccccc gccagatgtt
    26881 tgctcgccag cgcatcacac agcgccggaa tatccaccac agtaccgcgc gaggcattaa
    26941 tcagcagcga gccgggcttc attagcgaaa tctctttcgc gcccatcata tttttggtgg
    27001 acggattctc tggtacatgc agactcacca catcgctcat attcagcagg tcagaaagat
    27061 gctgtacctg agtggcgttg cccagcggca gtttgttttc aatatcataa aagtaaacat
    27121 acattcccag cgattcagcc agaatgccca attgcgtacc aatatgaccg tagccgatga
    27181 tacccagctt tttgccgcgc gcttcaaaag aacccgccgc cagtttgttc cacacgccac
    27241 ggtgcgcttt agcattggct tccggcacgc cgcgcaatag cagcagcagt tcgccaatca
    27301 ccagctccgc aacagagcgc gtatttgaga acggtgcgtt aaataccggg atcccgcgct
    27361 ttgccgccgc atccagatca acctggtttg ttccgataca gaaacagcca atagcgacca
    27421 gtttttctgc ggcgttgatc acgtcttcag tcagatgggt acgggatcgc aggccgatga
    27481 agtgggcatc gcggatggat tcttttaatt gttcatcatc cagcgcgcct ttgtgaaatt
    27541 cgatgttggt gtaaccagct gcacgaaggc tttccagcgc cttttggtgc acgccttcta
    27601 ccagcagaaa cttaatcttg tctttctcca gcgatacctt tgccatttac ccaatcctgt
    27661 cttttgaaat gttgtgtgcg gatttgcatc cgcctttcaa catatcaaaa aataatattg
    27721 cggcaatatg aacgtttgcg tcgcgatgtt gaggaaatat cacgcaaagg taattcttgg
    27781 atgaatatgc tggtttagag gatttattta gagcaatcga caattgcctg gtaaaagcgt
    27841 gacacatgtc accaaattta atgaagagaa tttttttaac gggggaggtt cccccgtcag
    27901 atcatttcac aatggttttg acaccgtcag gtgtgccaat cagcgcaacg tccgcgccac
    27961 ggttagcaaa caagccaaca gtcaccacgc caggaatcgc atttatggcg ttttccatcg
    28021 ctatcgggtc aaggatttcc atgccgtgga cgtcgaggat cacgttgcca ttatcggtca
    28081 ccacgccctg acggtattcc ggacgaccgc ccagtttcac cagctggcgc gccactgcac
    28141 tacgtgccat cgggataact tctactggca gcgggaattt acccagaata tcaacctgct
    28201 tggaggcgtc ggcaatacag ataaattttt ccgcaaccga agcaatgatt ttttcacggg
    28261 tcagcgccgc gccgccgcct ttgatcattt gcatgtggcc gttgatttca tctgcgccat
    28321 caacgtagat gccaaggctg tcgacttcgt tgagatcaaa aacgtgaatg ccgaggcttt
    28381 tcagtttttc agtggaagca tctgaactgg aaacggcccc ttcaatctgg cctttcatcg
    28441 tgccgagcgc gtcaataaag tgtgcggcgg tggaacctgt acctacacca acaatggtac
    28501 cgggctgaac atactgaagt gccgcccatc ctactgcttt tttcaattca tcctgcgtca
    28561 tgatcgtttc gcctgtggta tgaaatttca cacgcattat atacaaaaaa agcgattcag
    28621 accccgttgg caagccgggt ggttaactca tccataaaat atcgcgcaat ggcgggcatc
    28681 ccctttcgcc ccgcaaataa agcatacaac ggtctgggta tgccgctcca cggtgcgaac
    28741 agaggcaata acgccacgcc attcaacata accagggcgc gaagtcgcta agcgaaatgc
    28801 aatagcgcaa atccctgatc ttttaagctg cgcgtttaac cattgataca aatgttaccc
    28861 gccgacggct tcggtatatg caacctgaca caaaattgtg tcatagtgca ggaaaaagca
    28921 tttaccagga gcagacaaca gcaatgaaac gcccggacta cagaacatta caggcactgg
    28981 atgcggtgat acgtgaacga ggatttgagc gcgcggcaca aaagctgtgc attacacaat
    29041 cagccgtctc acagcgcatt aagcaactgg aaaatatgtt cgggcagccg ctgttggtgc
    29101 gtaccgtacc gccgcgcccg acggaacaag ggcaaaaact gctggcactg ctgcgccagg
    29161 tggagttgct ggaagaagag tggctgggcg atgaacaaac cggttcgact ccgctgctgc
    29221 tttcactggc ggtcaacgcc gacagtctgg cgacgtggtt gcttccggca ctggctcctg
    29281 tgttggctga ttcgcctatc cgcctcaact tgcaggtaga agatgaaacc cgtactcagg
    29341 aacgtctgcg ccgcggcgaa gtggtcggcg cggtgagtat tcaacatcag gcgctgccga
    29401 gttgtcttgt cgataaactc ggtgcgctcg actatctgtt cgtcagctca aaaccgtttg
    29461 ccgaaaaata ttttcctaac ggcgtaacgc gttcggcatt attgaaagcg ccagtggtcg
    29521 cgtttgacca tcttgacgat atgcaccagg cctttttgca gcaaaacttc gatcttcctc
    29581 ctggcagcgt accctgccat atcgttaatt cttcagaagc gttcgtacaa cttgctcgcc
    29641 agggcaccac ctgctgtatg atcccgcacc tgcaaatcga gaaagagctg gccagcggtg
    29701 aactgattga cttaacgcct gggctatttc aacgacggat gctctactgg caccgctttg
    29761 ctcctgaaag ccgcatgatg cgtaaagtca ctgatgcgtt actcgattat ggtcacaaag
    29821 tccttcgtca ggattaatcc atcaaataat gcctgatagc acatatcagg cgttgtcctc
    29881 acttcttttt gtattccttg aatcacatca caaaatagac aaa
//
LOCUS       DAJTXY010000038        28195 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTXY010000038 DAJTXY010000000
VERSION     DAJTXY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.038"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..1188
                     /gene="pdeC"
                     /locus_tag="OO566_001809"
     CDS             <1..1188
                     /gene="pdeC"
                     /locus_tag="OO566_001809"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR6605119.1"
                     /translation="YRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQWGVY
                     DTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSYQRL
                     ITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQPIID
                     IKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGAYLA
                     THADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMTPII
                     LAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHIIE
                     LAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPARELT
                     RGQ"
     gene            complement(1191..1514)
                     /gene="soxS"
                     /locus_tag="OO566_001810"
     CDS             complement(1191..1514)
                     /gene="soxS"
                     /locus_tag="OO566_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6605120.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1600..2064
                     /gene="soxR"
                     /locus_tag="OO566_001811"
     CDS             1600..2064
                     /gene="soxR"
                     /locus_tag="OO566_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6605121.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2768..4117
                     /gene="ghxP"
                     /locus_tag="OO566_001812"
                     /gene_synonym="yjcD"
     CDS             2768..4117
                     /gene="ghxP"
                     /locus_tag="OO566_001812"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6605122.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4268..5917
                     /locus_tag="OO566_001813"
     CDS             4268..5917
                     /locus_tag="OO566_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6605123.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6071..7363)
                     /locus_tag="OO566_001814"
     CDS             complement(6071..7363)
                     /locus_tag="OO566_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6605124.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKENDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7541..9190)
                     /gene="actP"
                     /locus_tag="OO566_001815"
     CDS             complement(7541..9190)
                     /gene="actP"
                     /locus_tag="OO566_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6605125.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9187..9501)
                     /locus_tag="OO566_001816"
     CDS             complement(9187..9501)
                     /locus_tag="OO566_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6605126.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9701..11659)
                     /gene="acs"
                     /locus_tag="OO566_001817"
     CDS             complement(9701..11659)
                     /gene="acs"
                     /locus_tag="OO566_001817"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR6605127.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12052..13488
                     /gene="nrfA"
                     /locus_tag="OO566_001818"
     CDS             12052..13488
                     /gene="nrfA"
                     /locus_tag="OO566_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6605128.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDKQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13533..14099
                     /gene="nrfB"
                     /locus_tag="OO566_001819"
     CDS             13533..14099
                     /gene="nrfB"
                     /locus_tag="OO566_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6605129.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14096..14767
                     /gene="nrfC"
                     /locus_tag="OO566_001820"
     CDS             14096..14767
                     /gene="nrfC"
                     /locus_tag="OO566_001820"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6605130.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14764..15720
                     /gene="nrfD"
                     /locus_tag="OO566_001821"
     CDS             14764..15720
                     /gene="nrfD"
                     /locus_tag="OO566_001821"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6605131.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            15740..17458
                     /locus_tag="OO566_001822"
     CDS             15740..17458
                     /locus_tag="OO566_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6605132.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSMLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17451..17834
                     /gene="nrfF"
                     /locus_tag="OO566_001823"
     CDS             17451..17834
                     /gene="nrfF"
                     /locus_tag="OO566_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6605133.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17831..18427
                     /gene="nrfG"
                     /locus_tag="OO566_001824"
     CDS             17831..18427
                     /gene="nrfG"
                     /locus_tag="OO566_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6605134.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18769..20082
                     /gene="gltP"
                     /locus_tag="OO566_001825"
     CDS             18769..20082
                     /gene="gltP"
                     /locus_tag="OO566_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6605135.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20160..20849)
                     /locus_tag="OO566_001826"
     CDS             complement(20160..20849)
                     /locus_tag="OO566_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6605136.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20943..23090)
                     /gene="fdhF"
                     /locus_tag="OO566_001827"
                     /pseudo
     CDS             complement(20943..23090)
                     /gene="fdhF"
                     /locus_tag="OO566_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23288..24754)
                     /gene="mdtP"
                     /locus_tag="OO566_001828"
     CDS             complement(23288..24754)
                     /gene="mdtP"
                     /locus_tag="OO566_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6605137.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24751..26802)
                     /gene="mdtO"
                     /locus_tag="OO566_001829"
                     /pseudo
     CDS             complement(24751..26802)
                     /gene="mdtO"
                     /locus_tag="OO566_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26802..27740)
                     /locus_tag="OO566_001830"
     CDS             complement(26802..27740)
                     /locus_tag="OO566_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6605138.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADIALTKLHLEMTTVRAPFDGRIISLKTSVGQFASAMRPIFTLIDTRHWYVIA
                     NFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSI
                     NWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ"
     gene            complement(27759..28034)
                     /locus_tag="OO566_001831"
     CDS             complement(27759..28034)
                     /locus_tag="OO566_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605139.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         6425 a         7134 c         7584 g         7052 t
ORIGIN      
        1 taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg gggaaattat
       61 gtggcggtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc gacattgcaa
      121 tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga ggcctcggca
      181 gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa tggctattta
      241 tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac ttcatatcaa
      301 cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg tattttgggg
      361 agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc tcccaaacgt
      421 aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc aataatcgat
      481 atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg tgagcagggg
      541 caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat gatagaacag
      601 ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct ggcaacacat
      661 gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc acggttgata
      721 gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat taaatttgaa
      781 gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct ggctttccgc
      841 caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa cttgcataac
      901 cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga aacgctgacc
      961 acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca cagcctgggg
     1021 ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct gcgcaaacgc
     1081 ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca ggtgtttatg
     1141 caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa ctacaggcgg
     1201 tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga gaaggtctgc
     1261 tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt ggtgcgcaac
     1321 tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt ctgatgcgtc
     1381 accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt tgcgacgaca
     1441 tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc ctgaataatt
     1501 ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc atctgttggg
     1561 gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa attaccccgc
     1621 attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc ggtatcggcg
     1681 ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg caatcagcgg
     1741 cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca gcgtattggc
     1801 attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca tacgttaagt
     1861 gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg gcgcattcat
     1921 accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg cctttcgcgc
     1981 agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac cggcgcacgc
     2041 ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat cgttaactat
     2101 gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca ccctaaccct
     2161 ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca aacacaaaac
     2221 taaagcgcca caagggcgct ttagtttgtt ttccggtctt tgtctttctc tctatcccgc
     2281 tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg tggtggaggt
     2341 tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca gtgtaaaagt
     2401 atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca atccagaacg
     2461 gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc gcatcaattg
     2521 cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc ggttgaaaaa
     2581 gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta tgcgcggagc
     2641 taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctggagcag gaagataacg
     2701 tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc tcaggggatg
     2761 ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct ggtttaaaat
     2821 ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa cgtttctggc
     2881 gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct tcccgcctgc
     2941 ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga tgggtctgtg
     3001 ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta ccgcattcag
     3061 cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt tcctgatggg
     3121 ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc gcaacttgcc
     3181 gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc tcattgccgc
     3241 taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg cgctgggtga
     3301 tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca tcggcttgga
     3361 aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa ttgtcggttt
     3421 gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat tgagcgatga
     3481 aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc ctgtagtcct
     3541 gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa ctatccgcgc
     3601 tgtcgccggc caggcgaacc tgctggataa agatgggcag atcatcgacg gcgggaaagc
     3661 actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag ctccggcagc
     3721 ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt tgacggctat
     3781 caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt acctcgttcc
     3841 ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga gcaacgtggc
     3901 gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg cggtattcat
     3961 cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc tggtgattgg
     4021 tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta tcgccgtggc
     4081 gctggtgacc ttctatgcgg ggggctgggc tatctaatct ttcatcggat tctgaaaacg
     4141 ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt tgcgatattc
     4201 tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca tcgcaaacag
     4261 ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct cgctgtccgg
     4321 ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa tcgccatcgg
     4381 tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg aactcttttt
     4441 agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga cccgtgaatt
     4501 tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg tcaccgtggt
     4561 cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc ccgcctttgc
     4621 gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg ttggcgaagg
     4681 gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga atgacgcttc
     4741 cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga tcttcactgt
     4801 cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg ccggttttgt
     4861 ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg gtgatgaacc
     4921 cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga ttgccgaaca
     4981 tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca cccgctccgg
     5041 tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct gggcgatgct
     5101 ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc cgggtattct
     5161 ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga tgctgtttac
     5221 cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc tgtggacgat
     5281 gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt cgtggaccac
     5341 acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc tggccggtgt
     5401 gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct atgagctggt
     5461 gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga tgttgcctat
     5521 tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag aacgtattgc
     5581 gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg agcgtctggc
     5641 ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt ctcgcgtcat
     5701 tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg tgcaggaaga
     5761 gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg aactgtacca
     5821 cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc acgatctcga
     5881 tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg taaccaggcc
     5941 cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct cgggcgaaca
     6001 ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt ggctgttctt
     6061 cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata gcgactgcat
     6121 taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct tcaaagaatt
     6181 tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca cggagaatgg
     6241 aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga cgggtattaa
     6301 acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa ttggcattat
     6361 taaaattaac attagccata ttagagccgg tacatataac catttcgagg aatgtatttg
     6421 tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg attgctttgt
     6481 tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt ttagagaaac
     6541 taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga tttacctcag
     6601 ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata ttggctctat
     6661 tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga gttccgtcag
     6721 ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa agtatttttt
     6781 ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa atagcgtttt
     6841 gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc acgataccct
     6901 tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt tcgatagtat
     6961 tattatggac ggcgacatta tttataaaat ataccacctt aatggataca tcattttctt
     7021 tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca tataacgcta
     7081 tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt cctgatggta
     7141 aactatcata aattcttttc attttctgaa tcaagcctat cactttttca tgatcatcta
     7201 ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt ttgctgtaat
     7261 tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta tcgttaatct
     7321 ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat ccttataatt
     7381 aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc aaaaatcgct
     7441 cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat gttggagagg
     7501 aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg cggccttgct
     7561 caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca cgctcgcgcg
     7621 ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat gccacgctga
     7681 tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg aggatctgta
     7741 cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcccc agccagccgc
     7801 ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga atgatcggga
     7861 agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg ttctgattct
     7921 caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg atttttgata
     7981 cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac aagtcatgcg
     8041 aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata gtggcgaaag
     8101 caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc aggtgaacgg
     8161 ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc ggattcgcac
     8221 caacaagcat gatcgcgccg aagccgataa taaaggtcag aatatagaag tagcccataa
     8281 atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact gtaaagaagc
     8341 gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc agagagagcg
     8401 cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct ttcgggtgta
     8461 ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc atcaccataa
     8521 aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt acccaggtgg
     8581 tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc agcaccaccg
     8641 caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct accatctgag
     8701 cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata cgaatcggcc
     8761 cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg ttacgcagac
     8821 gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac gagtagatca
     8881 gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag gcggcggaca
     8941 tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg cctgcggtgt
     9001 agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg agcgtaaaca
     9061 cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc tctacggccc
     9121 cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc gccgtcagaa
     9181 ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt cgaattcgcc
     9241 gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca ctccaacacc
     9301 aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca gccagccggg
     9361 cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa tcgacaggat
     9421 ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt cttctatccg
     9481 ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat tgttttaatt
     9541 cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg taggccggat
     9601 aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc gtcttatcag
     9661 gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc atcgcgatag
     9721 cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc gaggtatcgc
     9781 ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata attttgccgg
     9841 agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc agcgggccaa
     9901 tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt tcctccccgt
     9961 gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga atacctacta
    10021 cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct gccgtcccca
    10081 gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag taatagccat
    10141 cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg gaaaaatagg
    10201 tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc caggagtcgg
    10261 tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta tcgaccagcg
    10321 ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg gtagcgccag
    10381 gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg accaccggac
    10441 atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta attggctcgc
    10501 ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct ttatcgcctt
    10561 ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc tgatgcttgt
    10621 ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca aacatcagcg
    10681 tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta acccagccca
    10741 catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat ttaaaggtca
    10801 gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt tttccggtag
    10861 aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc gcctggtgct
    10921 gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc tgccagtcga
    10981 tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg ttcgggtttt
    11041 ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc acgccttcgt
    11101 cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg gctttcggcg
    11161 agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc agcatcgcaa
    11221 ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt ttaatgccca
    11281 gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta tagctgatat
    11341 gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg ccgttttctt
    11401 gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg taccatttaa
    11461 tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt ttgatccagt
    11521 cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat tgttgataca
    11581 tcgcctcgta ctgctgaggg tttatcaggc aacggtctgc gatgttggca ggaatggtgt
    11641 gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat atgtggcata
    11701 agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag atcacgcaaa
    11761 agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca aaagttgatt
    11821 aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat actaactaac
    11881 tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca cagtgtggtt
    11941 atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta ctgtcgtgaa
    12001 aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc tatgacaagg
    12061 ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt tttcacttct
    12121 gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc gaagaatgaa
    12181 acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc ggaacagtca
    12241 gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc ggggtatccc
    12301 ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga tgtgcgtgaa
    12361 accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat ggcgtgctgg
    12421 agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga tggctacttc
    12481 cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg tgccgactgc
    12541 cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct ttcccgtccg
    12601 tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg acgtttcgac
    12661 aagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg caaaaacaaa
    12721 gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca gtattacgac
    12781 aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct gaaagcgcag
    12841 cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt gacctgtatc
    12901 gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga ccataaaatt
    12961 ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca ggataaagct
    13021 gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa aatcaaggtt
    13081 gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg cgctggatgc aggcgcgacg
    13141 gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg ttgggatctg
    13201 gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg gatgctcggt
    13261 acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct ggcgaccaaa
    13321 ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc ccagcaggcc
    13381 attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa aacggtgatc
    13441 ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc ccgttccgcc
    13501 tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg ttattaactg
    13561 ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct acccctgcgg
    13621 tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat gccgcctgtc
    13681 tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc gttatcaacc
    13741 cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca ccgcaacacc
    13801 gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc ggagagcaga
    13861 acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc tggccgcacg
    13921 atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca cagcaagata
    13981 cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc cacagcgatc
    14041 agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag cagccatgac
    14101 ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca gcggcaccgc
    14161 agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca tggtgcatga
    14221 cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag tgaacaaggt
    14281 gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg aatttcctga
    14341 tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc cgtgcgttga
    14401 cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg atgttaatcc
    14461 tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg tgcgctttat
    14521 ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca atttgcaggc
    14581 cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt ttggcaatct
    14641 ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt accgctacaa
    14701 gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg gggaggtgag
    14761 ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg gccgattgcc
    14821 atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt gctgttacgt
    14881 cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac gctgattgtc
    14941 gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac aagaccgtgg
    15001 accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat gggggtgatg
    15061 ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt tgaatatgat
    15121 ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa ggttctgagc
    15181 cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc ggtgctgctg
    15241 ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct caataacccg
    15301 agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt ggcgctgatc
    15361 gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt tgttcaccgg
    15421 atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt tgtcggtctg
    15481 gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg cggtttctgg
    15541 acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat gttgctcaaa
    15601 ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggcgtgtgg ggccagtctg
    15661 gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac ggtggcgtaa
    15721 gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc tggcgttgtt
    15781 gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag tgcggttgcg
    15841 ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg cgctcctgct
    15901 gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg tcatttatgt
    15961 cgcccaacat agctacagca tgctgtcgtg ggaactcaaa ctggcggcgg tgtggggcgg
    16021 tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg cgctgtttgc
    16081 ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg ttttatctct
    16141 catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg tgcggatatt
    16201 tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg gtcttatctt
    16261 tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga gcgtggcgct
    16321 ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct ggcgctgggc
    16381 gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt gggcctactg
    16441 cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct ctttattacc
    16501 ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc gggggatttt
    16561 ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc tgggcacctt
    16621 aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata acgtccgcgc
    16681 cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc tgtatggctg
    16741 gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg aaatgttaat
    16801 cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg gaacgcttta
    16861 tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc cgtattttaa
    16921 ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg cgacgtttgt
    16981 ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc tgttatttgc
    17041 cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt tacagccggg
    17101 tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc tgcaagccag
    17161 aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc aacgcattgg
    17221 tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga tggaaccgtc
    17281 aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa ccagagcgga
    17341 tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg ggggaggatt
    17401 gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg atgaataaag
    17461 ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt gtagacacct
    17521 ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc cagttacgtt
    17581 gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg gtcagtatgc
    17641 gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc attggctgga
    17701 tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt cagacgctgg
    17761 tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc tggcgagtga
    17821 gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac tcacgatatt
    17881 gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg cggtacgtgc
    17941 ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa ccccggaagc
    18001 gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg aacaatgggc
    18061 gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc tggcgtatcg
    18121 tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg cgacggtgct
    18181 ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg acaaagccct
    18241 cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg cgtttatgca
    18301 ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca actcaccccg
    18361 gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc agcggagatc
    18421 ggattaatca tctgttgatg aagattatgt cattgagctg cataaaaaat aatcgaatga
    18481 acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat aaaaatcttt
    18541 ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg cattaatgca
    18601 gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata ctgatcataa
    18661 gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt atgtcacgtt
    18721 ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat gaaaaatata
    18781 aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct cctgggaagc
    18841 tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct ctctccggcg
    18901 ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat ctccacgctg
    18961 gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg cgcgaaaacc
    19021 attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac tctggcgaac
    19081 gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga tatctcgaaa
    19141 tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg cacgattttg
    19201 tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct gccgatcatc
    19261 tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca tcgtgaaccg
    19321 ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca catggtgatg
    19381 cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa ctttggtttc
    19441 tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat tctgttcttc
    19501 gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg gagcctgatt
    19561 cgtattctga aagatgagct gattctggcg tactccactg ccagctctga aagcgtactg
    19621 ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac cagcttcgtg
    19681 gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag tattgccgct
    19741 attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat cattctggtg
    19801 ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc gtttgtggtg
    19861 ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt tattgctggt
    19921 gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa tgcgctggcg
    19981 gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct ggcttatgag
    20041 cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat tgccggggat
    20101 atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc aggcctacct
    20161 taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc catacagctc
    20221 aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc ctcaccgttt
    20281 aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc agagctgcgt
    20341 ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt ggcgtaaatc
    20401 aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc cgaggcgttt
    20461 tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt taccagaata
    20521 tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc tgcaagaact
    20581 tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc caacagcgcg
    20641 caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc tttgctcaaa
    20701 tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc cccggcctct
    20761 gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa gaatgtcaaa
    20821 aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag ggagtaacca
    20881 gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta gaaaggactg
    20941 tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc gtcaatcagg
    21001 tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca gtatttgtat
    21061 tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca ggcaccaatc
    21121 caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac ctgcgcacgg
    21181 gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc aatacccaga
    21241 cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag cgccgccagt
    21301 gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg caccgttgac
    21361 agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc gcaggtgaag
    21421 aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga agtcccctga
    21481 tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc cattttctcg
    21541 taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat ctcctgggtg
    21601 ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat ttgccagtcc
    21661 gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc acggtcagcc
    21721 gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac atccgccgcc
    21781 gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc ttcaaaggct
    21841 ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat aatgtacgcg
    21901 gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata gccggtatga
    21961 gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc cggatctttc
    22021 acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc accctgcacg
    22081 ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt accggtcagc
    22141 atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg ggttacaccc
    22201 atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc ctgacgaatc
    22261 tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc ttcaacgatt
    22321 ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc gtacagattt
    22381 tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc gtttttcagt
    22441 gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca gacgataatt
    22501 ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg ggaatccgcc
    22561 gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat agcattgctc
    22621 attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg tcagacacga
    22681 gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg cattacatag
    22741 ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc cggaccgtac
    22801 ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc tcaggaaaca
    22861 ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg cggggtcagg
    22921 atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag ggtaccctgg
    22981 ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac gaccaggttg
    23041 attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat cggtctcgct
    23101 ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt tatgccacat
    23161 tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc tgacgaaaaa
    23221 caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc atcacgcggc
    23281 agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc agcgatttca
    23341 tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac atctcttcgg
    23401 caagtactgg caaccgggct tcagtggcct gtaagcggct ggttaagccg cgctgatagg
    23461 cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc tgcatttctc
    23521 gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc accgcgttca
    23581 gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg ccttcgagat
    23641 tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg aagttgaact
    23701 ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg aaaaacgctt
    23761 tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct aatgacgcct
    23821 gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca taagagagtg
    23881 tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc ggcatatcgc
    23941 tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt tgccctttga
    24001 cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg aaaggcactt
    24061 gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa tcaatcacat
    24121 cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg taataaagct
    24181 gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc gcggcattat
    24241 gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca agatccagtc
    24301 cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc agtgcgggcg
    24361 catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg ttaagcatcc
    24421 ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc gattgcgctt
    24481 tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc cgttggatca
    24541 gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc cagccggagc
    24601 tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt tgatgaggcg
    24661 cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg agaatgctgc
    24721 acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg cctgttccgt
    24781 ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct ggcagacgtg
    24841 ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct cttcaagcgt
    24901 tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct ccagggcgtc
    24961 ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg ccgaattcca
    25021 ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt gcgaacgttc
    25081 aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc gttggcacat
    25141 ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat aagtgcgcag
    25201 agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac ccagcgcgcc
    25261 agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg tgtaaatcat
    25321 cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt ccaccaggtc
    25381 gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca ccatctgtgt
    25441 gccgatataa gaagagcgtt cagagctggt ggcaatctat gcgcccaaca ggaaaatcgg
    25501 tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca gtagcgtgaa
    25561 taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca tcttctggta
    25621 cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg tgtgaatgcc
    25681 ttcccagtcc acgccgctgt aaaaggtgta gcagatcaaa caggcgagca atgtttttac
    25741 cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg gcggtttggc
    25801 ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg tctggcagat
    25861 attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc gccagtcgct
    25921 ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg aggctaattt
    25981 ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt agcgattcag
    26041 cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc tttgagttcg
    26101 ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca gcgcctctct
    26161 ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa tggcatcatc
    26221 aagcagatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa accacagcac
    26281 gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc acagcgttaa
    26341 gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt aaatggcgac
    26401 aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa acatgcagcc
    26461 catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg accatttgta
    26521 gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga tcgccacaaa
    26581 tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg ataacgccac
    26641 aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta cccagagttg
    26701 cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa agaacgccag
    26761 cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg ctcaagatta
    26821 gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac catgattttg
    26881 accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt tggcaggccg
    26941 cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc cactttaccc
    27001 tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc tgagcgaata
    27061 tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt gtcgatgagg
    27121 gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag ggaaatgatc
    27181 cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt cagggcaata
    27241 tccgcttctg cggcgcgctg cgccgttctt gcacggtcaa cgtcttctgc tgagacaaaa
    27301 ccctctttca gtaatggctc ggtgcggcgt aatgtatctg tggcctgttt cgcggcggca
    27361 cgggcttttt ctaccgtggc attaaccgag tcggcaccaa actgttgggc gtcaacgcta
    27421 cgctgggtga gcataatttg cttatccagc gccgcgaggg aggcttcggc tttcgccagg
    27481 ttggcttcgt acgggcgcgg gtcgatgcgg aacagcaaat cgccctgttt caccgcctgg
    27541 ttgtcggtga ccgccagttc tacaatgcga ccgctgactt ccggcaccac atcaatggta
    27601 tctgctgacg cgtaagcgtc attagttgat ggcgcactgt ctacgcgcca gataacgaaa
    27661 acaagggcaa ccagcgccaa cgcaaccact aacagagcag ggaatttact gcgaggtgct
    27721 tttttcggcg tactttccat gagcgcgtct caaatagatt agaaaaatgc cagccagaac
    27781 agcatggcgt agagagcaaa aagggcggta taaataattc cgacaaatgc cagattgata
    27841 tttgcccgct gaataactcg cctcgtgatg agcgtcaaaa taaggctggc aatggcgcag
    27901 aaaaaccagc tgggataata agcgccgatc accgggattg ccggagataa tgagcatccc
    27961 gaaaccatga agacgggaat tatccaggct gtttttttga ggtgtatcgc catgcgagag
    28021 agaactgttg gcatattaaa tacatcctgt ctgctgcgaa caatatctgt agtgaatatc
    28081 aatatccatt gaaggcgtca gtatggctat tttctcgatg aataaaaata gctttatcca
    28141 gccaccgcat tcataactta tatattttat ttattaattg gctgtatata ttttt
//
LOCUS       DAJTXY010000039        27700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTXY010000039 DAJTXY010000000
VERSION     DAJTXY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.039"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            42..157
                     /gene="rrf"
                     /locus_tag="OO566_001832"
     rRNA            42..157
                     /gene="rrf"
                     /locus_tag="OO566_001832"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            483..1781
                     /gene="kgtP"
                     /locus_tag="OO566_001833"
     CDS             483..1781
                     /gene="kgtP"
                     /locus_tag="OO566_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6605140.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1778..2101)
                     /locus_tag="OO566_001834"
     CDS             complement(1778..2101)
                     /locus_tag="OO566_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6605141.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2147..3502)
                     /gene="pssA"
                     /locus_tag="OO566_001835"
     CDS             complement(2147..3502)
                     /gene="pssA"
                     /locus_tag="OO566_001835"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6605142.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3616..6276)
                     /gene="pat"
                     /locus_tag="OO566_001836"
     CDS             complement(3616..6276)
                     /gene="pat"
                     /locus_tag="OO566_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6605143.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLHDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6308..7006)
                     /gene="tapT"
                     /locus_tag="OO566_001837"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6308..7006)
                     /gene="tapT"
                     /locus_tag="OO566_001837"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6605144.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7075..7494)
                     /gene="trxC"
                     /locus_tag="OO566_001838"
     CDS             complement(7075..7494)
                     /gene="trxC"
                     /locus_tag="OO566_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6605145.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7701..8738
                     /locus_tag="OO566_001839"
     CDS             7701..8738
                     /locus_tag="OO566_001839"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6605146.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8786..9475)
                     /gene="ung"
                     /locus_tag="OO566_001840"
     CDS             complement(8786..9475)
                     /gene="ung"
                     /locus_tag="OO566_001840"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6605147.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9780..10163
                     /gene="grcA"
                     /locus_tag="OO566_001841"
     CDS             9780..10163
                     /gene="grcA"
                     /locus_tag="OO566_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6605148.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10219..10806)
                     /gene="eamB"
                     /locus_tag="OO566_001842"
     CDS             complement(10219..10806)
                     /gene="eamB"
                     /locus_tag="OO566_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6605149.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10909..11790
                     /locus_tag="OO566_001843"
     CDS             10909..11790
                     /locus_tag="OO566_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6605150.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11825..13159)
                     /gene="srmB"
                     /locus_tag="OO566_001844"
     CDS             complement(11825..13159)
                     /gene="srmB"
                     /locus_tag="OO566_001844"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6605151.1"
                     /translation="MTVTTFSELELDKSLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13291..14028
                     /gene="trmN"
                     /locus_tag="OO566_001845"
     CDS             13291..14028
                     /gene="trmN"
                     /locus_tag="OO566_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6605152.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14013..15635)
                     /gene="nadB"
                     /locus_tag="OO566_001846"
     CDS             complement(14013..15635)
                     /gene="nadB"
                     /locus_tag="OO566_001846"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR6605153.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15891..16046
                     /gene="rseD"
                     /locus_tag="OO566_001847"
     CDS             15891..16046
                     /gene="rseD"
                     /locus_tag="OO566_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6605154.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            16043..16618
                     /gene="rpoE"
                     /locus_tag="OO566_001848"
     CDS             16043..16618
                     /gene="rpoE"
                     /locus_tag="OO566_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6605155.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16651..17301
                     /gene="rseA"
                     /locus_tag="OO566_001849"
     CDS             16651..17301
                     /gene="rseA"
                     /locus_tag="OO566_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6605156.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17301..18257
                     /gene="rseB"
                     /locus_tag="OO566_001850"
     CDS             17301..18257
                     /gene="rseB"
                     /locus_tag="OO566_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6605157.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTLTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18254..18733
                     /gene="rseC"
                     /locus_tag="OO566_001851"
     CDS             18254..18733
                     /gene="rseC"
                     /locus_tag="OO566_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6605158.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18931..20730
                     /gene="lepA"
                     /locus_tag="OO566_001852"
     CDS             18931..20730
                     /gene="lepA"
                     /locus_tag="OO566_001852"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6605159.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20746..21720
                     /gene="lepB"
                     /locus_tag="OO566_001853"
     CDS             20746..21720
                     /gene="lepB"
                     /locus_tag="OO566_001853"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6605160.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21992..22672
                     /gene="rnc"
                     /locus_tag="OO566_001854"
     CDS             21992..22672
                     /gene="rnc"
                     /locus_tag="OO566_001854"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6605161.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22669..23574
                     /gene="era"
                     /locus_tag="OO566_001855"
     CDS             22669..23574
                     /gene="era"
                     /locus_tag="OO566_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6605162.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23586..24314
                     /gene="recO"
                     /locus_tag="OO566_001856"
     CDS             23586..24314
                     /gene="recO"
                     /locus_tag="OO566_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6605163.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24326..25057
                     /gene="pdxJ"
                     /locus_tag="OO566_001857"
     CDS             24326..25057
                     /gene="pdxJ"
                     /locus_tag="OO566_001857"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6605164.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            25057..25437
                     /locus_tag="OO566_001858"
     CDS             25057..25437
                     /locus_tag="OO566_001858"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6605165.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25857..25937
                     /gene="shoB"
                     /locus_tag="OO566_001859"
     CDS             25857..25937
                     /gene="shoB"
                     /locus_tag="OO566_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6605166.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(26131..26391)
                     /gene="yfhL"
                     /locus_tag="OO566_001860"
     CDS             complement(26131..26391)
                     /gene="yfhL"
                     /locus_tag="OO566_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6605167.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26606..>27700
                     /locus_tag="OO566_001861"
     CDS             26606..>27700
                     /locus_tag="OO566_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR6605168.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT         6837 a         6901 c         7270 g         6692 t
ORIGIN      
        1 ttaaatcaga acgcagaagc ggtctgataa aacagaatta gcctggcggc cgtagcgcgg
       61 tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg
      121 tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaactaagcg aaaggccatc
      181 cgtcaggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc
      241 ttatactccc acatatgaca gattcagcaa cggatgcggt ttccccaact tgcctacttc
      301 catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca ttttcctgct
      361 aactaaattc atggtttagg ttgcataatg atacgcaaca aatgtataac attttattta
      421 ccgaaagaaa taaacaaaag cgaccaacaa aagcatcgga ttacggcagg agacataatg
      481 gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat actcgtcgcc
      541 gcatttgggc gattgtgggg gcctcttcag gtaatctggt cgagtggttc gatttctatg
      601 tctactcgtt ctgttcactc tacttcgccc acattttctt cccttccggg aacacgacga
      661 ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc ccaataggcg
      721 gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg ctgttatcgg
      781 tgtgtatgat gtgtttcgga tcgctggtta tcgcctgcct cccaggttat gaaactatag
      841 gtacgtgggc tccggcatta ttgcttctcg ctcgtttatt tcagggatta tccgttggcg
      901 gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg cgcaaaggtt
      961 tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc ctactggttg
     1021 tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga tggcgtattc
     1081 ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt caattagatg
     1141 aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa ggattatggc
     1201 gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc ctttgtttct
     1261 ataccttcac cacttatatg cagaagtatc tggtaaatac tgcgggaatg catgccaacg
     1321 tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa ccactcattg
     1381 gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt tcgctggcag
     1441 ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct tatgccgctt
     1501 ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc agtggaatac
     1561 tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggtctg tcatatgcgg
     1621 tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg aaatcaatag
     1681 gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg tttctggttt
     1741 ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc agttgccaga
     1801 cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag ctccagccgc
     1861 tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca gagaacatca
     1921 atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat tcatttccgg
     1981 cggcggaaag tatcgccgag gcgataaagt gttgagcttt atcctgcccg ctccagctat
     2041 cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa agaatacgca
     2101 aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac aggatgcggc
     2161 taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga accttcaccg
     2221 gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta cgctcgcgga
     2281 tcagctccag ttctttctct cgctgcggcg caagctccag ttgtggatcg tggatcaaaa
     2341 tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg gtgatcaaca
     2401 tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca tctttccata
     2461 accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg aaacgacgca
     2521 gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca tcttccggaa
     2581 tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc ccttcgcgca
     2641 gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc gtacagatgg
     2701 ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac agactcgatt
     2761 tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg ccctggaaat
     2821 gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg atttccgggc
     2881 tttttggccg attaacatca tccagacgat taacgccgcg gccattcata atattctgtg
     2941 taacccattc aaacataatg tctgacatct tacggttacg gatcagatga taacggtcgt
     3001 agcgatattt atcgtgttga tgcaggtaaa catcgttcag gctggcaccg ctataaagta
     3061 cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagagcttca cgggtattga
     3121 ttggaacgcc ataaaccgga acatctacgc ccggattttc ctgcgccatg cggcagtacc
     3181 agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca cgatgccagt
     3241 cgaccagcac ccgcacatcc agttccggac gctgcctttt agcctcatac aacgcgttca
     3301 gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa atgcgctgct
     3361 tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg taaaagaaat
     3421 cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt tgatgtttat
     3481 tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga agggtcctct
     3541 gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa gtggtcaaca
     3601 tttccagtac cttactcatg attcctcgcg ccgggcaaga tttagcgtaa gcccaacgat
     3661 cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg ccaccatgcc
     3721 acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatttc gcgtataggt
     3781 aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg agcgaaccag
     3841 tacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga cgccgaggat
     3901 ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat cgtagtcgat
     3961 ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa agtagcggta
     4021 ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct catcttctgg
     4081 caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt cttccagctg
     4141 atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg gcgagatatc
     4201 cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat gaatatccag
     4261 acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa gctggctcaa
     4321 gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac ttttgatccc
     4381 ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga cgacggcttg
     4441 atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc cgaaaaccgg
     4501 atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca tactttgcac
     4561 caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga aaatagcgtt
     4621 cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct gaacttccga
     4681 tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa tctgttcggc
     4741 aatatgcacc gcttcggtgc tatcgctggc aatccaggtt gggagcgtgt tcatgccata
     4801 cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt cggcaatcgc
     4861 ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg gcaacgccgg
     4921 cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa aagcagtgat
     4981 ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac gtcgtgcctc
     5041 ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc tgcggggatg
     5101 atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag cggcgctggg
     5161 cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt ccagcgtttt
     5221 gacataatgc tcgctgctgg cgtcatcgcg cagatcgagc gggttagaga ctgccacatg
     5281 ttctggcaat gcatcgtgca atttctggca ggtttcttcg cttagcgttg ccagcttgcc
     5341 attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt tgctgataat
     5401 catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg ccgaaaacag
     5461 ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat cccatgccgg
     5521 gtccattcct gccgtggtgt tgagcagtcg ctgtgccgcc gggctacgtc cgcttttaat
     5581 caccagaatc ggtttattac gcgaggcact acgggccgcc gaaacaaagc gtcgcgcgtc
     5641 gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg ccagatagtc
     5701 gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt aggaaaagcc
     5761 catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggcagccg attgcgaaat
     5821 aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca gaccttgcca
     5881 gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc gcagggcgca
     5941 ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg ttttacagcc
     6001 tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac ataaaaccgc
     6061 aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac ccaacaccgc
     6121 tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cctcccgcca gcaggttacg
     6181 catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg ctatcgattt
     6241 tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca gaagtgaccg
     6301 aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt tgcgtttttc
     6361 ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct gccgccccgg
     6421 tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat tggcctgcag
     6481 cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa atgacgggaa
     6541 gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag gtaccatcga
     6601 gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc cgttgctcat
     6661 cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt accagatcca
     6721 gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca ggcaaaatat
     6781 cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac atcagcaaac
     6841 agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat ttctctggca
     6901 aaagacagcg ttggcagcga cgaacgcgat taccgcgggc aagaaaagga cgtgttgcgc
     6961 gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga gagcgtcgaa
     7021 aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcgccccggt aagattaaag
     7081 agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa gcatgtcgac
     7141 aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa agcgactgct
     7201 caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc tacgctcttg
     7261 cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg gtgcccagaa
     7321 gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac cggtcgcatt
     7381 aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg catcttcgat
     7441 ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat tcataactaa
     7501 cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta atatgttaca
     7561 tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta tgatagtcgc
     7621 aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc gcgcttcgcg
     7681 cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc aaggtcaaag
     7741 tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg cgtaccggga
     7801 aagggggcgg gcgtccagga aaatctcgtg ctgatggtgg ccgtcgcccc gcccgcgatg
     7861 acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc acggtttccc
     7921 gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt ggtaaaagtt
     7981 ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc tacggcgaaa
     8041 acgcctgtca ggcactgttc cagagccgtc cggaagcgat tgttcgcgcc tggtttatcc
     8101 agagtgtaac gccgcgtttt aaagaagcct tgcgctggat ggcagcaaac cgcaaagcgt
     8161 accatgtggt ggatgaagcg gaattgacaa aagcgtcagg cacggaacat cacggcggcg
     8221 tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta atccaggcag
     8281 gcgcgcagga ttgcgttctg gcactggaaa acgaatctaa cccgcataac ctgggtggca
     8341 tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat gcggcactgc
     8401 tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt cagccgatta
     8461 ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac accgtagtga
     8521 ccacttccag cgagcagggt aaaccgctgt tcaaaaccag tctgccagcg aaaatggtac
     8581 tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg aacgatctgc
     8641 gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc gcaaccggcg
     8701 ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg gtggcaactc
     8761 tgccatccgg catttccccg caaatttact cactctctgc cggtaatact ggcatccagt
     8821 caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa tggttgcagc
     8881 caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt acatgatggc
     8941 gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac aaaaacacca
     9001 cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc tcccaaccga
     9061 ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg agcagcagaa
     9121 cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag cccggaatag
     9181 tattttccag ctctttatac atattcaata atgacggagg aatggcaatg ccgggacgaa
     9241 cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg ccgagaatca
     9301 ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct ttttgtggtg
     9361 gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta ttaagaaaat
     9421 aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta gccatcttgc
     9481 tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa caaaatcatg
     9541 ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt tcacacccgc
     9601 ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt ggtttttgtt
     9661 gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag caacaatggt
     9721 tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg aggcatcaca
     9781 tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac tctttctggc
     9841 tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt tatgcagaag
     9901 atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt ccagtagaag
     9961 tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt ctgcgtcgtg
    10021 aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc atccgtgtat
    10081 ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac gttatcgctc
    10141 gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga agagggcgac
    10201 ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca gcaacagggc
    10261 aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca gatgccccgc
    10321 cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc tgacgccaac
    10381 tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg taacaccgta
    10441 caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc tgattggttt
    10501 tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca gccagacaat
    10561 atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca ctgccagtga
    10621 aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca tccctgccag
    10681 cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga gaatattgtt
    10741 cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta aaaggatcgg
    10801 tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca ttaaaaaaaa
    10861 tgaatgtttt tatattctaa attcgatatt ttcgataggt ttggggttat ggatctgcgc
    10921 cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc ttcgcaaaaa
    10981 ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca ggagttttca
    11041 gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg aaaaaagttg
    11101 ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc tgccaaaaag
    11161 gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct ctcttaccga
    11221 atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt atcgttgcag
    11281 gcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga tgtcggcgtc
    11341 ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga acaatcgctg
    11401 gtgctggtgg cttcaccaca aattgcagat gtcgatttta ctgagccggg aagacataac
    11461 gcctgtagct ttattatcaa cgaaccgcaa tgtgtcttcc ggcagatatt tgagagcacg
    11521 ctgcgacagc ggcggatcac ggtggaaaac accattgagc ttataagtat cgaaagcatc
    11581 aagcgttgtg tcgcggcgaa tatcggtgtc agttacttgc cccactttgc ggtggaaaaa
    11641 gagcttgaga gcggagaatt aattgaattg ccttttggcg aacagtcgca gaccattacg
    11701 gcaatgtgcg cacatcatgc tggaaaagcg gttagcccgg cgatgcacat ttttattcag
    11761 tgtgttgaag agtgttttgt ggttgcgtaa aaaaacaatg ccgggcttaa acccggcatt
    11821 gagattactc ttctgtcgtt tgcggtggca cgcccgttcc gctcggttta cggcgcttac
    11881 caatattttt ggtgtcgcga tggcgttttt tcacccgcgg cttctctttc tctttagctt
    11941 ttttcttctc ggcgcgtttt gccagtactt tcttcgatgg cttgccggtc tgcttctcgc
    12001 ttggcgcacg cgttttcggg cgtaactcat caataacgcg agctttaatc ggctcttcaa
    12061 tgtaacggcc aaccttgccc agcagcaaat ggtcatgggc ttccaccagc gaaattgcgg
    12121 tgcctttacg accggcgcgc gcggtacgtc cgatacgatg caaataagta tcgccactgc
    12181 gcggcatatc gaagttaaag acgtggctga cgtcaggaat gtcgataccg cgcgcggcaa
    12241 catcggtggc aaccagtacg tttacgcggc cttcggtcag gcgtttaatt gcttcgttac
    12301 gcttgccttg taccatctca ccttcgagat agcagttgtt gatgcccgct tcgcgcagcc
    12361 agtttgccag ctcatgcaca cgttcacgct tacgcacaaa cacgattgag cgagtcgctt
    12421 caggctgttt taacaggtgc accagcaatg cggttttatg ttccaggtca tcggcgcggt
    12481 aataccactg atgaattttt ttgcgctcac gggtggacgg attggcagaa acttccaccg
    12541 gatcttccag cagacgctcg gcaaagtcct gaatcgcatc gccttccagc gttgccgaaa
    12601 agagcagggt ctgtttacgc cagcgagttt cgccagcaat atgttcgata tcctgagcga
    12661 agcccatatc cagcatacgg tctgcttcgt cgaggatcag cgtttcaacc gcgcggcaat
    12721 cgaagttctc ttcttttatg tattgcagca gacgtccggt cgtggcgacc acgatgtcct
    12781 gattttcgct gaacacttcc gcgtggttca tataggctac gccgccggtg atggtggcga
    12841 tatccagatg cgtatgtttc gccagttcgc gggcatgatc ggccacctgc atcgccagct
    12901 cgcgagttgg ggtgaggatc aaaatacgcg gcggaccgga tttcttacgt gggaaatcga
    12961 gcaggtgctg caacgctggc agcagatacg ccgccgtttt accggtgcct gtcggcgcag
    13021 aaccgagtac atcacggcca tcgagcgcag gcggaatggc ggcagcctga atggcggtcg
    13081 ggcgagtgaa acctttatcc tggagggctt ccagcaggct tttgtcgagt tcaagttcgg
    13141 aaaaagtcgt tacagtcatg ttctacctct gtgtggggcg ctgattatag acgttacggc
    13201 tgcaatcttc atctgtttgt atggatatcg cttctcgggt attgttctca cccggtgcta
    13261 tcgccgtttt tcctgcaagg ttttattttc atgtcacagt ctacatccgt gcttcgtcgt
    13321 aatggattta cttttaaaca gttttttgtt gctcacgatc gctgtgcgat gaaagtggga
    13381 acggatggca ttttgctggg cgcatgggca ccggtggctg gggtgaaacg ttgccttgat
    13441 atcggcgcgg gtagcgggtt gctggcactg atgctggcgc agcgaaccag tgacagcgtg
    13501 ataattgatg ccgttgagct ggaaagtgaa gctgccgcgc aggcgcagga aaatatcaac
    13561 cagtccccgt gggcagagcg gattaacgtc catacggcgg atattcagca gtggatcaca
    13621 cagcagacag tacgcttcga tttaatcatc agtaacccac cttactatca gcagggagta
    13681 gagtgcgcga cacctcaacg ggaacaggct cgctatacca ctacgcttga tcacccatcg
    13741 ctgctgacct gtgcggcgga gtgcattacc gaagagggat ttttctgcgt ggtgctgcca
    13801 gagcagattg gtaatggttt tactgaactg gcattaagca tgggctggca tttacgtttg
    13861 cggacggatg tggcggaaaa cgaagcgcga ctgccgcatc gggtgctgct ggcattctcc
    13921 ccgcaggcgg gagaatgctt tagcgatcgc ttagtcattc gtgggccaga ccagaactat
    13981 tccgaagcgt atacggcgct gacccaggct ttttatctgt ttatgtaatg attgccgggg
    14041 gaaaggatcg atggaccgga atgcgtgagc agttccgggt aatccagcgt aaaatgcagc
    14101 ccccggcttt ctttgcgcat catcgcgcag cgaacaatca actcggcaac ctgtaccaga
    14161 ttacgcagct ccagcaaatt atttgagacg cggaaatggg cgtaatattc gtctatttct
    14221 tgttggagca tggttatccg ccgcagggcg cgttccaggc gcttcgttgt gcgcacaatg
    14281 ccaacgtaat cccacataaa cagccgtagc tcgtgccagt tatgctgaat tactacccgt
    14341 tcgtcagggt tctcaacgcg gctttcatcc cacggcggta acgtactgac gccgtgggca
    14401 taaggcatac gtctggtgat atcttctgcc gccgaccagc catagaccag acactccagc
    14461 aatgaattcg aggccatgcg gttagcgccg tgtaagccgg tataactcac ctcgccaatg
    14521 gcatacaagc cctcgacgtc cgtacgccca tgatcatcaa ccattacacc accgcaggta
    14581 tagtgtgcag caggcacaat cggtaccggt tcttgtgtga gatcaatccc cagcccgagc
    14641 aatttttcat aaatcatcgg gaaatgctgg cgaataaaat cggcgggctt atggctgatg
    14701 tcgaggaaca tacaatctgc gccgaggcgt ttcatttcat ggtcaatggc gcgggcgaca
    14761 atatcgcgcg gggccagttc gccgcgctca tcaaaatcgg gcataaaacg cgtgccatcc
    14821 gggcgcttga gataagcgcc ttcgccgcgc agtgcttccg ttaacaggaa attgcgtgcc
    14881 tgtgggtgat acagtgcggt agggtggaac tgattaaatt cgagattggc aacccgacag
    14941 cccgcgcgcc acgccatagc aatgccatcg ccagaagaaa tatccggatt ggtggtgtac
    15001 tgatacacct tcgacgcacc gccggttgcc agtaccactg cttttgcgtg gcaggtttcc
    15061 accgtttctt tatttcggtt ccatacccac gcgcctacaa cccgtcgcgt gcccggcagg
    15121 ccaattttgt cagagacaat cagatcaact gcgttgctgc gctccagcac acgaatattc
    15181 ggatggttct gcgccttgct caccagcgtg gattgtactt ctctaccggt ggcgtcggcg
    15241 gcatgaagaa tacgacggtg actatgtcca ccttcacggg tcagatggta gctttcttcg
    15301 ccattcggtt gaacgtgggt atcaaacaaa accccctggt caattaacca ttgcacacag
    15361 gaacgagcat tgctggcgac aaattcaact gcatggcgat cgcaaatacc agccccggaa
    15421 atcaatgtgt cttccacatg cgagtcaatg ctgtcagttt catcaaacac ggcggcaata
    15481 ccgccctggg cataaaatgt tgaaccttcc gttaccgggc ctttacttag aacgatgacc
    15541 tgatgctggt cagccaggcg tagcgccagt gaaagtccgg ctgcgccgct accaataatc
    15601 aacacgtcac atgaatgttc agggagagta ttcattttct ttgtttaatc tactaaacat
    15661 tgtttggtca gcatagcatc atgttgtgcg aataaacacc tgctatttta atatttgtta
    15721 cagtggctaa acacgttgac gcagggcggc gagaaaaaga gaagttactg gctggtggag
    15781 gattaggtgg tgaaataaaa aggccgttgg gttactcttc aggcagttaa atgggcattt
    15841 ctacacagat aatgcgatgt tcagattctg tagacttata atgatagata atgctccgtc
    15901 tacagcatga caaacaaaaa cagatgcgtt acggaacttt acaaaaacga gacactctaa
    15961 ccctttgctt gctcaaattg cagctaatgg agtggcgttt cgatagcgcg tggaaatttg
    16021 gtttggggag actttacctc ggatgagcga gcagttaacg gaccaggtcc tggttgaacg
    16081 ggtccagaag ggagatcaga aagcctttaa cttactggta gtgcgctatc agcataaagt
    16141 ggcgagtctg gtttcccgct atgtgccgtc gggtgatgtt cccgatgtgg tacaagaagc
    16201 ttttattaaa gcctatcgtg cgctggattc gttccgggga gatagcgctt tttatacatg
    16261 gctgtatcgg attgctgtaa atacagcgaa aaattacctg gttgctcagg ggcgtcgtcc
    16321 accttccagt gatgtggatg ccattgaagc tgaaaacttc gaaagtggcg gcgcgttgaa
    16381 agaaatttcg aaccctgaga acttaatgtt gtcagaagaa ctgagacaga tagttttccg
    16441 aactattgag ttcctcccgg aagatttgcg catggcaata accttgcggg agctggatgg
    16501 cctgagctat gaagagatag ccgctatcat ggattgtccg gtaggtacgg tgcgttcacg
    16561 tatcttccga gcgagggaag ctattgataa caaagttcaa ccgcttatca ggcgttgacg
    16621 atagcgggat actggataag ggtattaggc atgcagaaag aacaactttc cgctttaatg
    16681 gatggcgaaa cgctggatag tgagctgctt aacgaactgg ctcataaccc agaaatgcag
    16741 aaaacctggg aaagctatca cttaatccgt gattcaatgc ggggtgatac tcccgaggtg
    16801 ctccatttcg atatctcttc acgcgtgatg gccgccattg aagaagagcc agtacgtcaa
    16861 tcggcgacat tgatcccgga agcccagcct gcgccgcatc aatggcagaa aatgccattc
    16921 tggcagaaag tacgtccgtg ggcggcacag cttacccaaa tgggcgtagc cgcatgcgta
    16981 tcgcttgcag ttatcgttgg cgtccagcac tataatggac aatctgaaac gtcccagcag
    17041 cccgaaacgc cggtatttaa tacactgccg atgatgggta aagccagccc ggtaagcctg
    17101 ggagtacctt ctgaagcgac cgcaaacaat ggtcaacagc agcaggtaca ggagcagcgt
    17161 cgtcgcatta atgcaatgtt gcaggattac gaactgcaac gccgactcca ctctgaacag
    17221 cttcagtttg agcaggcgca aacccagcaa gccgctgtac aggtgccagg aattcaaact
    17281 ttaggaacgc aatcgcagta atgaagcaac tttggtttgc catgtcatta gtgacaggta
    17341 gcctgttatt ctctgctaac gcctcggcca ctcccgcgtc cggggcgtta ttacagcaga
    17401 tgaacctggc cagtcagtca ctgaattacg agctgtcatt catcagcatc aataaacagg
    17461 gtgttgagtc tctgcgttat cgacatgcac gcctcgataa ccgtcctctt gcacaattgt
    17521 tgcaaatgga tggcccgcgc cgggaagtgg tacagcgcgg caatgaaatc agctattttg
    17581 aaccgggact tgaaccgttc acgcttaatg gcgattacat tgttgattct ctgccatcgc
    17641 ttatctatac cgatttcaaa cgcctttctc cttactacga ctttatctcc gtcgggcgca
    17701 cgcgtattgc tgatcgtctt tgcgaagtca ttcgcgtggt tgcccgagat ggtacacgct
    17761 acagctacat cgtgtggatg gacaccgaat cgaaattacc gatgcgggtt gatctccttg
    17821 atcgcgatgg tgaaacgctg gaacaatttc gcgtgattgc ttttaacgtc aatcaggata
    17881 tcagcagcag tatgcagacg ctggcgaagg caaatttgcc gccgttgctt tctgttcctg
    17941 taggtgaaaa agctaaattc agctggacgc taacttggtt gccacagggt tttagcgaag
    18001 tttccagtag tcgacgtccg ctaccgacga tggacaacat gcctatcgaa tcacgtctct
    18061 attccgacgg attattcagc ttctcggtaa acgttaaccg cgctacgcca tcgagcaccg
    18121 atcagatgtt gcgcaccgga cgcagaaccg tcagtacaag cgtacgtgat aacgccgaaa
    18181 tcaccattgt cggtgaactg ccgccgcaaa cggcgaaacg cattgccgag aatattaagt
    18241 tcggggcagc gcaatgatca aagagtgggc taccgtcgtc tcctggcaaa acgggcaggc
    18301 gctggtcagt tgtgatgtta aagcttcatg cagcagctgc gcttcacgcg ccggttgcgg
    18361 tagccgcgtg ttgaataaac ttggcccgca aaccacgcat accattgtcg taccctgtga
    18421 tgagccgtta gtgccggggc aaaaagtgga attggggatc gccgaaggca gcctgcttag
    18481 ctccgcatta ctggtttata tgtcgccgct ggtgggatta ttcctcatcg cttcgctatt
    18541 tcagctactc tttgcttctg acgtcgcagc attatgtggt gcggtcctcg gcggtattgg
    18601 cgggttcctg attgcgcgcg gctactcgcg taagtttgct gcccgggcag aatggcaacc
    18661 gatcatctta agcgtggcat tgccgcctgg ccttgtgcga tttgaaacat cttcggaaga
    18721 cgcgagccag tgatttcact ggctttgttg cattttgcat aacaaatgcg agggcgtttc
    18781 cagataccac aatccatacg gtatgaacat ttcctcgcct caatgttgta gtgtagaatg
    18841 cggcgtttct attaatacag acgttaagct cagaacagcg acttctaaaa gcctggttaa
    18901 ccgggcatta aggcacaata atcatacttt atgaagaata tacgtaactt ttcgatcata
    18961 gctcacattg accacggtaa atcgacgctg tctgaccgta ttatccagat ctgcggtggc
    19021 ctgtctgacc gtgaaatgga ggcgcaggtt ctcgattcca tggatcttga gcgtgagcgt
    19081 ggcattacca tcaaagcgca aagcgtgacg ctggactaca aagcgtctga cggcgaaacc
    19141 tatcagctta actttatcga caccccgggc cacgtagact tctcctatga agtttcccgt
    19201 tcgctggctg cctgtgaagg tgcattgctg gtggtcgacg ccgggcaggg cgtagaagcg
    19261 caaaccctgg caaactgcta caccgccatg gaaatggatc tcgaagtggt gccggtactg
    19321 aacaagattg acctgccggc agccgatcct gaacgcgtgg cggaagaaat tgaagatatc
    19381 gtcggcatcg acgccaccga tgcagtgcgc tgttcagcga aaaccggcgt tggcgtgcag
    19441 gacgttctcg aacgtctggt acgcgacatt ccgccgccgg aaggcgatcc ggaaggcccg
    19501 ttgcaggcac taattatcga ctcctggttc gacaactacc tgggcgttgt ttcacttatc
    19561 cgtattaaaa acggcaccct gcgtaagggc gacaaagtga aagtcatgag taccgggcag
    19621 acctataacg ccgaccgtct gggcatcttc acgccgaaac aggttgaccg cactgaactg
    19681 aaatgtggcg aagtaggctg gctcgtatgt gcgattaaag atatccacgg cgctccagtc
    19741 ggcgatacct taacgctggc gcgtaatccg gcagaaaagg cgctgcctgg ctttaagaaa
    19801 gtcaaaccgc aggtatacgc cggtctgttc ccggtaagtt ccgacgacta tgaagccttc
    19861 cgtgacgcgc tgggtaaact cagcctgaac gatgcctcac tgttctatga gccggaaagc
    19921 tccagcgcgc tgggctttgg tttccgctgc ggcttccttg gcctgctgca catggagatc
    19981 atccaggagc gtctggaacg tgaatacgat ctggatctga tcaccactgc gccgaccgta
    20041 gtgtatgaag ttgaaaccac gtcaagggaa gttatctacg tcgacagccc atccaagctg
    20101 cctgcggtaa ataacattta cgaactgcgc gagccgattg cagagtgtca catgctgctg
    20161 ccgcaggcat atctcggcaa cgttattacg ctgtgcgtag aaaaacgcgg cgtgcagacc
    20221 aatatggttt accacggtaa tcaggtggcg ctgacgtacg agatcccgat ggcggaagtg
    20281 gtgctcgact tcttcgatcg cctgaaatct acctcgcgtg gttatgcgtc tctggattac
    20341 aacttcaaac gcttccaggc gtccgacatg gtacgtgtag acgtattaat caacggtgaa
    20401 cgtgttgatg cgctggcgtt gatcacccac cgtgataatt cgcaaaaccg cggtcgcgag
    20461 ttggtggaga agatgaaaga tctgatccca cgccagcagt ttgatatcgc cattcaggca
    20521 gcgattggta cgcacattat tgcgcgatcc accgtgaaac agctgcgtaa aaacgtactg
    20581 gctaaatgtt atggcggcga tatcagccgt aagaaaaagc tgctgcagaa gcagaaagaa
    20641 ggtaagaaac gcatgaagca gatcggtaac gttgagctgc cgcaggaagc gttcctcgcc
    20701 attctgcacg tcggcaaaga caacaaataa cccttaggag ttggcatggc gaatatgttt
    20761 gccctgattc tggtgattgc cacactggtg acgggcattt tatggtgcgt ggataaattc
    20821 tttttcgcac ctaaacggcg ggaacgtcag gcagcggcgc aggcggctgc cggtgactca
    20881 ctggataaag caacgttgaa aaaggttgca ccgaagcctg gctggctgga aaccggagct
    20941 tctgtttttc cggtgctggc tatcgtattg attgtacgtt cgtttattta tgaaccgttc
    21001 cagatcccgt caggttcgat gatgccgact ctgttaatcg gtgattttat tctggtagag
    21061 aagtttgctt atggcattaa agatcctatc taccagaaaa cgctgatcga aaccggtcat
    21121 ccgaaacgcg gcgatatcgt ggtctttaaa tatccggaag atccaaagct tgattacatc
    21181 aagcgcgcgg tgggtttacc gggcgataaa gtcacttacg atccggtctc aaaagagctg
    21241 acgattcaac cgggatgcag ttccggccag gcgtgtgaaa acgcgctgcc ggtcacctac
    21301 tcaaacgtgg aaccgagcga tttcgttcag accttctcac gccgtaatgg tggggaagcg
    21361 accagcggat tctttgaagt gccgaaaaac gaaaccaaag aaaatggaat tcgtctttcc
    21421 gagcgtaaag agacactggg tgatgtgacg caccgaattc tgacagtgcc gattgcgcag
    21481 gaccaggtgg ggatgtatta ccagcagcca gggcaacaac tggcaacctg gattgttccg
    21541 ccgggacaat acttcatgat gggcgacaac cgcgacaaca gcgcggacag ccgttactgg
    21601 ggctttgtgc ctgaagcgaa tctggtcggt cgggccacgg ctatctggat gagcttcgat
    21661 aagcaagaag gcgaatggcc gactggtgtg cgcttaagtc gcattggcgg catccattaa
    21721 tagccatctt cgttcacgtt gtcgccgtta tggcgacaac gtgaattatt tatgagataa
    21781 atctcccgtg gctaacgaca tcccccgtcg ttgtgtatag aatattcccc cgaagtttaa
    21841 ggttggcacc tccaggttgc cacggcacac gaaacagcgt tggtccccat ataccgctaa
    21901 actgaaactg cagcgaagca gttagcagag ccatgtatat caggtctgtt tcgtgtgctg
    21961 aattgttgac gcatttattt attggtatcg catgaacccc atcgtaatta atcggcttca
    22021 acggaagctg ggctacactt ttaatcatca ggaactgttg cagcaggcat taactcatcg
    22081 tagtgccagc agcaaacata acgagcgttt agaattttta ggcgactcta ttctgagcta
    22141 cgttatcgcc aatgcgcttt atcaccgttt ccctcgtgtg gatgaaggcg atatgagccg
    22201 gatgcgcgcc acgctggtcc gtggcaatac gctggcggaa ctggcgcgcg aatttgagtt
    22261 aggcgagtgc ttacgtttag ggccaggtga acttaaaagc ggtggatttc gtcgtgagtc
    22321 aattctcgcc gacaccgtcg aagcattaat tggtggcgta ttcctcgaca gtgatattca
    22381 aaccgtcgag aaattaatcc ttaactggta tcaaactcgt ctggacgaaa ttagcccagg
    22441 cgataaacaa aaagatccga aaacgcgctt gcaagaatat ttgcagggtc gccatctgcc
    22501 gctgccgact tatctggtag tccaggtacg tggcgaagcg cacgatcagg aatttactat
    22561 ccactgccag gtcagcggcc tgagtgaacc ggtggttggc acaggttcaa gccgtcgtaa
    22621 ggctgagcag gctgccgccg aacaggcgtt gaaaaaactg gagctggaat gagcatcgat
    22681 aaaagttact gcggatttat tgccatcgtc ggacgtccga acgttggcaa atccacattg
    22741 ttgaacaaac tgctggggca gaaaatctcc atcacctccc gcaaggcgca gacaactcgt
    22801 caccgcattg tggggatcca tactgaaggc gcgtatcagg cgatctacgt cgatacaccg
    22861 ggcctgcata tggaagaaaa acgcgccatt aaccgcctga tgaacaaagc ggcgagcagc
    22921 tctattggcg atgttgagct ggtgattttt gtcgttgaag gcacccgctg gacgccggac
    22981 gacgaaatgg tgctcaacaa actgcgcgaa ggcaaagcgc cggtaatcct cgcggtgaac
    23041 aaagtggaca acgtgcagga gaaagccgat ctgctgccgc acctgcagtt cttggcaagc
    23101 cagatgaact tcctcgatat cgtgccaatc tctgccgaaa ccgggctgaa tgttgacact
    23161 attgcggcaa tcgtgcgtaa gcatctgcct gaagcgactc atcacttccc ggaagattac
    23221 atcaccgatc gctcacagcg ttttatggcg tctgaaatca tccgcgaaaa actgatgcgt
    23281 ttccttggcg ctgaactgcc gtactccgtg accgtggaga tcgaacgttt cgtctctaac
    23341 gaacgcggcg gttatgacat caacggtttg attctcgttg agcgtgaagg gcagaagaag
    23401 atggttattg gcaacaaagg ggccaagatc aaaaccatcg ggattgaagc gcgtaaagac
    23461 atacaggaaa tgttcgaagc gcctgtccac cttgaactgt gggtaaaagt gaaatcgggt
    23521 tgggccgacg acgaacgcgc actgcgcagt ctcggttatg ttgacgatct ttaagagtca
    23581 ctccgatgga aggctggcag cgcgcatttg tcctgcatag tcgcccgtgg agcgaaacca
    23641 gcctgatgct ggacgtcttc acagaggaat cgggtcgcgt gcgtctggtt gccaaaggcg
    23701 cacgctctaa acgctctact ctgaaaggtg cattacagcc tttcacccct ctcttgctac
    23761 gttttggcgg gcgtggcgaa gtcaaaacgc tgcgcagtgc tgaagctgtc tcactggcgc
    23821 tgccattaag cggtatcacg ctttacagcg gtctgtacat caacgaactt ctctcccgcg
    23881 tactggaata cgagacgcgc ttctctgaac tctttttcga ttacttgcac tgcattcagt
    23941 ctcttgcagg tgccactggt acgccagaac ccgcgctgcg tcgctttgaa ctggcactac
    24001 tcgggcatct gggctatggc gtcaatttta cccattgcgc gggtagcggc gagccggtag
    24061 atgacaccat gacgtatcgt tatcgcgaag aaaaagggtt tatcgcaagc gtcgttatcg
    24121 acaataaaac gttcaccgga aggcagttaa aagcgttaaa cgcgcgggaa tttcctgacg
    24181 cagacacact acgcgccgcg aaacgcttta cccgaatggc gcttaagccg tatcttggcg
    24241 gtaaaccatt aaagagcagg gaactgttcc ggcagtttat gcctaagcga acggtgaaaa
    24301 cacattatga atgatgagga ttgtcatggc tgaattactg ttaggcgtca acattgacca
    24361 tattgcaacg ctgcgtaacg cacgcggtac cgcttacccg gatccggtgc aggcggcgtt
    24421 tattgccgag caggcgggag cggacggcat taccgtgcat ttacgtgaag atcgccgcca
    24481 cattactgac cgcgatgtgc gcatcctgcg gcagacgctg gatacccgaa tgaatctgga
    24541 gatggcagtg accgaagaga tgctggcgat cgccgttgag acgaagccac atttttgctg
    24601 cttggtaccg gaaaagcgtc aggaagtaac aaccgaaggc ggcctggacg ttgcagggca
    24661 gcgtgaaaaa atacgcgatg cctgcaaacg tctggcagat gccgggattc aggtttctct
    24721 gtttatcgac gccgatgaag agcagatcaa agctgcggca gaggttggcg caccgtttat
    24781 cgagatccac accggttgct atgctgatgc caaaactgac gccgaacagg cgcaagagct
    24841 ggtgcgtatc gccaaagccg cgacctttgc cgctagtctc ggtctgaaag ttaacgccgg
    24901 acacggtctg acctatcaca acgtgaaagc cattgccgcc atccctgaga tgcatgaact
    24961 gaatatcggt catgccatta ttggccgtgc agtgatgacc ggactgaaag atgcggtggc
    25021 agaaatgaag cgtctgatgc tggaagcgcg tggctaatgg caatattagg tttaggcacg
    25081 gatatcgtag agatcgctcg catagaagca gtgatcgccc gatccggtga gcgcctggca
    25141 cgccgcgtat taagcgataa cgaatgggca atctggaaaa cgcaccacca gccggtgcgt
    25201 tttctggcga agcgttttgc tgtgaaagaa gccgcagcaa aagcgtttgg caccgggatc
    25261 cgcaatggtc tggcgtttaa tcaatttgaa gtattcaatg atgagctcgg caaaccacgg
    25321 ctacgactat ggggtgaggc attaaaactg gcggaaaagc tcggcgttgt aaatatgcat
    25381 gtaacgctgg cagatgaacg ccactatgct tgtgccacgg taattattga aagttaaaat
    25441 aataccgttc tctttaaaaa gtaaaacccc cgaactggta aggaacgggg gttttacttt
    25501 ttaactttaa ttttgtgcag catgcaccct caacaacagc gattatggat cagtgtactt
    25561 atcttttaca agcaacttgc aatctttagc ataaaaactc gagcctttac gaagaaagca
    25621 atattgatgg aaagattaac gtgaccgcca attcgtaagt acattaaaat tggcttcgtt
    25681 attgaagatt ttgctgtgct ttacaccatg ccacagaatt cccccattga aacgagtggt
    25741 gtcgtcaaag ctctggtgtg gagtgcagca tgcaccctca ataactcgca cgttcagttt
    25801 tggggagatg taagggctaa tctgaatggc tgcattcctt gtttaaggaa aaacgaatga
    25861 ctgattgccg atacctgatt aaacgggtca tcaaaatcat cattgctgtt ttacagctga
    25921 tccttctgtt cttataacac aaggaaacgt acttaaggtg cgtccggtga accaatcgga
    25981 cgcgccttta ataactataa ataagtgtct tggcagatac tatataaatt aacttagtga
    26041 atgattatgc taatgtcatc aattaaataa atataatggc gttaaggctt cccagtaata
    26101 taattaatac tctacttcca gagtagaata ttaaatttta tccgcgtggt gcatcagcac
    26161 aaatttatcc cacaactgtt cttctgtctc gacatgcgcc ggatctttca caatagtatt
    26221 ggggatcggg cacaccttct ggcaggttgg tgtctcgtag tgccctacgc attcggtaca
    26281 cttatcgctg ttaatttcgt agatatgatc tcccattgaa atcgcctcat tcgggcattc
    26341 gggttcacac atatcacaat taatacagcg tttagtaatt agtaaagaca tttcaatgga
    26401 ttaccattaa atcattttaa aatcagtaag ttgtatcgag tttgtatgct ttactgtcat
    26461 taacttactg tatgttgatc cagtgtattt caccttgata aactcagtcc agcaacacaa
    26521 aaccgcaaca cattgcattt tgtcccgtag aaaagacttg tatgtgtgag cttgttttct
    26581 gcgcctacgc agataaggat tgagaatgcc gcgcactgta acacataatc cggatagccc
    26641 caataatgac gatgttttag ccgcatctga aaaatgggac gcctgtaaac ccccctatac
    26701 cagcgcacac atgaaaatct gtgttgctgc cgccaaaatc atcctcgctg cttccggagt
    26761 ggctcgccgt tccaaatacg aaaaagagaa ctatctccgt atcgatttca gcaaagccgg
    26821 taaggttaca ttttacgccg agtttccaaa aaagatgggc ctcaaaggta aaaagctcgg
    26881 cgagtggccg gagctcgcta tccagctggc gcgcgaaaaa gcactaggta tggctgacgg
    26941 tggactgcgg gcagagtccg tacatgcagc gctggaaatg taccgggatg acctcaaagc
    27001 caaagtagcc cggcagaagc tgagcccgga cagtttcaca acctacgggg tgcgtatcga
    27061 ccggattaaa gcaacgttcg gcgagcgtga ggtgttcagc gacgtaacat acaatcggct
    27121 ggtggaagtg ctggacgagt ggatcgccac tcgctcgaac aataacgccc tggagttgtt
    27181 tgccgagctc cgtcggttct ggaagttctg cgcacctact ctttgcaacg gccgcaatgt
    27241 tgccgccagt ctgccagatg attatgtttc cttccgcgta cagaaaccta cccccacacg
    27301 gctttttacc gatattgaat caatcgcccg actctggctc aatgttgctg cctgcacctc
    27361 tgtacaccag aagaatgctg ttcgcttcat gatcatcact ggtgttcgtc cgattaatgt
    27421 ccataacctg cgctgggact acgtttacga ggaggctggt gaaattgttt atccggaagg
    27481 ggttatcggc atgcgagggg ctatgaaaac acaaaaggct ttccgcctgc cgataacgcc
    27541 tgagatccgg cggattatcg acgagcagaa agcctggcgt gattcagttc ctgagtgcaa
    27601 cagggattat gtatttttgc agccacgtga tccaatgcag ccattttcaa aacgatcact
    27661 ggataagctg gtgaaaacat acagcccgga cggggctgta
//
LOCUS       DAJTXY010000040        27403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTXY010000040 DAJTXY010000000
VERSION     DAJTXY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.040"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..1090)
                     /gene="ycjM"
                     /locus_tag="OO566_001862"
     CDS             complement(<1..1090)
                     /gene="ycjM"
                     /locus_tag="OO566_001862"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR6605169.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQ"
     gene            complement(1302..1649)
                     /gene="pspE"
                     /locus_tag="OO566_001863"
     CDS             complement(1302..1649)
                     /gene="pspE"
                     /locus_tag="OO566_001863"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6605170.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1692..1913)
                     /gene="pspD"
                     /locus_tag="OO566_001864"
     CDS             complement(1692..1913)
                     /gene="pspD"
                     /locus_tag="OO566_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6605171.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1922..2281)
                     /gene="pspC"
                     /locus_tag="OO566_001865"
     CDS             complement(1922..2281)
                     /gene="pspC"
                     /locus_tag="OO566_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6605172.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2281..2505)
                     /gene="pspB"
                     /locus_tag="OO566_001866"
     CDS             complement(2281..2505)
                     /gene="pspB"
                     /locus_tag="OO566_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6605173.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2559..3227)
                     /gene="pspA"
                     /locus_tag="OO566_001867"
     CDS             complement(2559..3227)
                     /gene="pspA"
                     /locus_tag="OO566_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6605174.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3394..4371
                     /gene="pspF"
                     /locus_tag="OO566_001868"
     CDS             3394..4371
                     /gene="pspF"
                     /locus_tag="OO566_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6605175.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCATLNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4491..5756)
                     /gene="puuE"
                     /locus_tag="OO566_001869"
     CDS             complement(4491..5756)
                     /gene="puuE"
                     /locus_tag="OO566_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR6605176.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHSDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5794..7074)
                     /gene="puuB"
                     /locus_tag="OO566_001870"
     CDS             complement(5794..7074)
                     /gene="puuB"
                     /locus_tag="OO566_001870"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6605177.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7076..8564)
                     /gene="puuC"
                     /locus_tag="OO566_001871"
                     /pseudo
     CDS             complement(7076..8564)
                     /gene="puuC"
                     /locus_tag="OO566_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(8714..9271)
                     /gene="puuR"
                     /locus_tag="OO566_001872"
     CDS             complement(8714..9271)
                     /gene="puuR"
                     /locus_tag="OO566_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6605178.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNVN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9298..10062)
                     /gene="puuD"
                     /locus_tag="OO566_001873"
     CDS             complement(9298..10062)
                     /gene="puuD"
                     /locus_tag="OO566_001873"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6605179.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10274..11692
                     /gene="puuA"
                     /locus_tag="OO566_001874"
     CDS             10274..11692
                     /gene="puuA"
                     /locus_tag="OO566_001874"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6605180.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11841..12005
                     /locus_tag="OO566_001875"
     CDS             11841..12005
                     /locus_tag="OO566_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605181.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11995..13380
                     /gene="puuP"
                     /locus_tag="OO566_001876"
     CDS             11995..13380
                     /gene="puuP"
                     /locus_tag="OO566_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6605182.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13514..13759
                     /locus_tag="OO566_001877"
     CDS             13514..13759
                     /locus_tag="OO566_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6605183.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14072..15715
                     /gene="sapA"
                     /locus_tag="OO566_001878"
     CDS             14072..15715
                     /gene="sapA"
                     /locus_tag="OO566_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6605184.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVDVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15712..16677
                     /gene="sapB"
                     /locus_tag="OO566_001879"
     CDS             15712..16677
                     /gene="sapB"
                     /locus_tag="OO566_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6605185.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16664..17554
                     /gene="sapC"
                     /locus_tag="OO566_001880"
     CDS             16664..17554
                     /gene="sapC"
                     /locus_tag="OO566_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6605186.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17554..18546
                     /gene="sapD"
                     /locus_tag="OO566_001881"
     CDS             17554..18546
                     /gene="sapD"
                     /locus_tag="OO566_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6605187.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18548..19354
                     /gene="sapF"
                     /locus_tag="OO566_001882"
     CDS             18548..19354
                     /gene="sapF"
                     /locus_tag="OO566_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6605188.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19422..19775
                     /locus_tag="OO566_001883"
     CDS             19422..19775
                     /locus_tag="OO566_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6605189.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20143..20931
                     /gene="fabI"
                     /locus_tag="OO566_001884"
     CDS             20143..20931
                     /gene="fabI"
                     /locus_tag="OO566_001884"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6605190.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21077..22204
                     /locus_tag="OO566_001885"
     CDS             21077..22204
                     /locus_tag="OO566_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6605191.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRENNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22272..24206
                     /locus_tag="OO566_001886"
     CDS             22272..24206
                     /locus_tag="OO566_001886"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6605192.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24284..24386)
                     /locus_tag="OO566_001887"
                     /pseudo
     CDS             complement(24284..24386)
                     /locus_tag="OO566_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24440..26414
                     /gene="pdeR"
                     /locus_tag="OO566_001888"
                     /pseudo
     CDS             24440..26414
                     /gene="pdeR"
                     /locus_tag="OO566_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26562..26735
                     /locus_tag="OO566_001889"
     CDS             26562..26735
                     /locus_tag="OO566_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605193.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26825..>27403
                     /locus_tag="OO566_001890"
     CDS             26825..>27403
                     /locus_tag="OO566_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6605194.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEK"
BASE COUNT         6339 a         7118 c         7060 g         6886 t
ORIGIN      
        1 cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag ccacgcagtg
       61 ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt ttactggagg
      121 gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt tgcaccgcat
      181 gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca tcaccgttgc
      241 caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaatgatc actgtacctg
      301 gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt ttttccagat
      361 ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg cggacatact
      421 cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac acttcagggc
      481 tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg cgcgtggaat
      541 tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggacgggtg acggcactga
      601 gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc tgttgtaaat
      661 agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca aacattaaat
      721 gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg gcgacctgat
      781 gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt aataaatgaa
      841 catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg ggtagtggtt
      901 ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca ctttcatccc
      961 agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc gttacaagtt
     1021 tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccaggtaa tccgtaattt
     1081 tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact actccgcaga
     1141 aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt ttttaccagg
     1201 gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata aggtgtgaca
     1261 gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt gaccttcggc
     1321 attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat ctcgctaagg
     1381 atctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac ggtgtcgttt
     1441 ttatccggaa ctgcggtggc aatgcgctct ttcacttcct tcaggggaat attgatggcc
     1501 ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca gtgttcagcg
     1561 gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt taaacatgta
     1621 agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta acaatttttg
     1681 cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg acttaacagc
     1741 ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga ttttatcgcc
     1801 cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag cttgcctgct
     1861 aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt cataactttc
     1921 ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca taacgttcca
     1981 tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc agcaattcgc
     2041 tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca agcgcaaatg
     2101 acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaagaaaatc gacagcacca
     2161 ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg gcgcagacgc
     2221 cgcggaccat gccctgctgt ggaatacgcc ataatttttt attgagatta atgcccgcca
     2281 ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg cctgaatacg
     2341 ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct cactttgcga
     2401 caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg gtaaaacaaa
     2461 cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta ctccttagac
     2521 gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc ttgcttcatt
     2581 ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag ttcggcaaac
     2641 tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc ttccatctgg
     2701 tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt gccactgtcc
     2761 agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat caatgcctgc
     2821 tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt cttcatgcgt
     2881 gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag atcggtcagt
     2941 ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag cagcgccagt
     3001 tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg gcgagtcagc
     3061 tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag tgtatcttcc
     3121 atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc taacagagcg
     3181 ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat gttgtcctct
     3241 tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt gccaattttt
     3301 tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc aggatgaatt
     3361 agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga taatttactt
     3421 ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc accgctggac
     3481 aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc cagccgcctg
     3541 cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc gacgttaaat
     3601 gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac cggtgcgcaa
     3661 aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct tgatgaactc
     3721 gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta cggtgaactg
     3781 gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg cgcgacgaat
     3841 gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct cgaccggctg
     3901 gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat tatgctgatg
     3961 gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt cccggggttt
     4021 acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat tcgtgaattg
     4081 aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc gcttgatgac
     4141 atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt ttcagaaaat
     4201 acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca ggaaaaagag
     4261 ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc tgaattactg
     4321 gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta gcgaagttga
     4381 gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa tcaatacgtc
     4441 gtttgcaggc ctaataagcg cagcgcatca gacattattg cgttgggcta ttaatcgctc
     4501 agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat ggtcagcgga
     4561 tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag cccctgcgcc
     4621 agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt ttgcggatcg
     4681 ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc cggaacgctt
     4741 tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc gcgttcgcag
     4801 agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc cagcgggttc
     4861 ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt cgcgttaccg
     4921 accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat taagtccggc
     4981 ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc gctttgcact
     5041 tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc ggcaaccagc
     5101 tcttttggcg caacgttgaa accgccctcg ccctgcaccg gttcgaaaat aatggccgcg
     5161 acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag ggagtcctgt
     5221 gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc agggaacggg
     5281 ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat atatgtacga
     5341 ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc gcgagcaatt
     5401 ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt agcctgcccg
     5461 ctcactgggg caagggcgtt aattttctcc gccagcgtga cgtagctttc gtacggcaca
     5521 atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc gaccagatca
     5581 gagtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat gtattcgttg
     5641 ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt acacatcacg
     5701 ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt gctcatgttt
     5761 cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat cgcgcaggct
     5821 gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc cggggaacgg
     5881 gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca gcagttcagc
     5941 aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac cctgcatgta
     6001 atagatgttg gtatcaaggc ggccgaactg cggcatgcgc gacaaggtca gcaggaagtt
     6061 gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt ttagtagttt
     6121 cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta cacccccgcc
     6181 gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt tacagtcttc
     6241 cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc gttcggtggt
     6301 gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta ctttatcgcc
     6361 cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat tggcagtccg
     6421 aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat acacgcgccc
     6481 gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt gaatatgccc
     6541 accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc ggcgaatagc
     6601 gttggcgtcc agcagctcca gctgtttatt gccgtagcgt tcccagttct ctttctgctc
     6661 ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg ggcggtagtc
     6721 gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct cgaacatcat
     6781 gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt cgatgtcgcg
     6841 gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc cgatgcgtga
     6901 ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg aggagagccc
     6961 ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca gtgtatcgaa
     7021 tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg tcatttcagg
     7081 cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca tgcagggatt
     7141 tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata tcgccgtcgt
     7201 tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg tgcgcgcggg
     7261 agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg gcaagctgca
     7321 gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa atctcttcgc
     7381 ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg gccgctgcca
     7441 gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca cgaataaagg
     7501 tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt gcgggatcaa
     7561 gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag aattcatcgg
     7621 cgatgctctc ttccagtaac aaacgcgttc cggcgatgca cacctggccc tggttgtaga
     7681 agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg gcgaaaacga
     7741 tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta tcacccgcgt
     7801 ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca tcgatatcgt
     7861 tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg ttcaacacac
     7921 catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg agcggtgatt
     7981 tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg agtttccagc
     8041 aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg accggttcac
     8101 gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac actttgtcga
     8161 tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca cgcagactgt
     8221 gacgaatccg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc ggcgtgggct
     8281 tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga agagagtgac
     8341 cagtcgccgc gttcaaatac gccgcgagct gcgctcaccg cacggtcgat atcgacgctc
     8401 ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt ttcaaaggtt
     8461 tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc aatggcgagg
     8521 cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac tcctgtttca
     8581 cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg ggctgcatgc
     8641 ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt acccggctta
     8701 cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg caaatacctg
     8761 ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt tgccccgcga
     8821 cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt actgtgccta
     8881 tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg tacgtttcaa
     8941 agatcatcgc cagcgtgcga ttcgggttaa cgttatgaac cagcttcatt gacaccccct
     9001 gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca tcaggttttt
     9061 ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg
     9121 tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc
     9181 cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc
     9241 gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca
     9301 gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct cgaacaatat
     9361 acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa
     9421 aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg
     9481 caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc aaaagttgct
     9541 acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt catgcgacgg
     9601 tgcatattgc tgttccaccg gcagttcggg atcttcccga tgctccagca attcaggctg
     9661 ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt gtaaaccccg
     9721 gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg ccatgctcag
     9781 aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg
     9841 cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa gttgttcaag
     9901 taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg cgtggatgat
     9961 ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc tgttcctgca
    10021 cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc tcacaaagtg
    10081 gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat tttttcaatc
    10141 tagcagtgga ttttcatttt tgcaaaccaa atttaacatt tgacaaacat ttggtttgca
    10201 tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga aaacaaacca
    10261 aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga
    10321 agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa
    10381 tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg gaaaacgtat
    10441 ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct cggtatttgc
    10501 aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa tgggcgagcc
    10561 ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg atccagagtt
    10621 tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc
    10681 gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt tcccggttgt
    10741 agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag gggatctgca
    10801 accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt actccgttga
    10861 taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc agttgcagct
    10921 gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa tcaaccttta
    10981 ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac gcctggtgcg
    11041 tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca
    11101 cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg agaatgtgct
    11161 ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg ggatgattga
    11221 cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc gccgcttcca
    11281 gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct
    11341 gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg cgggtgccga
    11401 tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg gccttgataa
    11461 cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag gcttaccctt
    11521 cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc tgcgccgcta
    11581 tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt
    11641 tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt aattcccctt
    11701 gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca
    11761 tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac
    11821 tgtgacaagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggaggctgcg
    11881 tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg ctaaacaggt
    11941 ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc aattatggct
    12001 attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg
    12061 aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac cgtatttgat
    12121 acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta tttgctggcg
    12181 ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggag
    12241 gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg atttatggtc
    12301 ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt gttggcgaaa
    12361 atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc
    12421 gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt caataccctg
    12481 tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt tcagggactg
    12541 cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag cgagaacgcg
    12601 cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct cggtttcgat
    12661 gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc
    12721 ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt tatgcagctg
    12781 ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga aattgcgctg
    12841 tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt gaacacatta
    12901 gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac
    12961 aatgtgtttc cagagcgcgt gtttggctat gtgcacccaa aatggcggac tccggcactc
    13021 aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt agtcaccgcg
    13081 acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt
    13141 aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt ccactatttg
    13201 ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct agagtcaacg
    13261 tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg gtacctgatc
    13321 cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag cgaaacgtaa
    13381 tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca ttaactataa
    13441 aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc
    13501 ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg ccgatgagta
    13561 cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg cccactactt
    13621 ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag cccaacatga
    13681 aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa
    13741 tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg ttgtccggat
    13801 aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct ttaatgggtt
    13861 tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga
    13921 aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac caaccacacg
    13981 gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag
    14041 atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt ctcttttggt
    14101 gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga
    14161 tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc
    14221 caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct
    14281 ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct gggaagtact
    14341 cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga
    14401 ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat
    14461 ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct acttcgacag
    14521 cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata ccgttgagtt
    14581 ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt atgcttcggt
    14641 catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc aactcgaccg
    14701 tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc aatttattcg
    14761 cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg tagtggtgga
    14821 tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat gcgacgttct
    14881 ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct tgcgtttaac
    14941 gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac cgccgctaaa
    15001 taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc tgatgcaatc
    15061 catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct cgtgggccta
    15121 tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac agttgaaggc
    15181 gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa
    15241 ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg ttgacgtaaa
    15301 agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg atatgagcca
    15361 tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt tcttccgtcc
    15421 gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt gcgatccgaa
    15481 attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc gtattgaagc
    15541 ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc tggcgtcgtc
    15601 attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc cgtttggtaa
    15661 cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac catgattatc
    15721 ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc
    15781 ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc
    15841 tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag cattaacggt
    15901 caaccaattg ccgaacagtt aaaagaggtc ttcccggcga cgatagaact gtgcatcctc
    15961 gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg gattacgcgc
    16021 cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc aatcccggtg
    16081 ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg gttgcccgtt
    16141 tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc gttgattgat
    16201 gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat ccgccatatg
    16261 atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt
    16321 atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac ccgcggtttg
    16381 tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc ggttattcct
    16441 cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga aatggttttt
    16501 agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga ttacgcagcc
    16561 atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat
    16621 attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt
    16681 atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga
    16741 cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt gtatttttgg
    16801 cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat tattgccgcc
    16861 atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc tcggacgtga
    16921 tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac
    16981 gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga cacacggcct
    17041 ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt cgctgctgtt
    17101 ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg
    17161 gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg acgaactgga
    17221 aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata ttctctggtt
    17281 tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat
    17341 ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc
    17401 tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt
    17461 catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc ttggcgatgg
    17521 cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg
    17581 accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt aagtatgacc
    17641 ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt
    17701 gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga ccgtatgcgt
    17761 tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat
    17821 aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt
    17881 cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg gcagcgtttt
    17941 ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa agatcacaaa
    18001 gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa agtgatgata
    18061 gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac aaactcaatg
    18121 gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca aaacagcaat
    18181 accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt
    18241 aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt ggtgacgatg
    18301 ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg
    18361 ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga acagttaccg
    18421 attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat tgtgacgcca
    18481 cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa catggagaaa
    18541 gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg
    18601 gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct ttacgctacg
    18661 tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca cactggcaaa
    18721 aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg atcatccgct
    18781 gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc aggatccttc
    18841 gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac tgcgcctgaa
    18901 caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc gtatggttgg
    18961 gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac aaaaacagcg
    19021 tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg atgaagccct
    19081 cgcttcactg gatatgtcga tgcgttcgca gttgattaat ctgatgctgg agttacagga
    19141 aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag
    19201 cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca ccgcggatgt
    19261 gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact ttggcgaagc
    19321 attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct tccagattat
    19381 tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat aaaataaaat
    19441 caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc tggcgatatc
    19501 ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg gggagctaca
    19561 ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt gggcagcatg
    19621 atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag ggctggaccg
    19681 tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg gagattattc
    19741 tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc gaccgagccg
    19801 aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca aggtctgaac
    19861 catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt tgctggggta
    19921 acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg gtaaggggaa
    19981 aagcgccccc cctgccgcag cctgctccgg tcggacctgg caactatagc tactcacagc
    20041 caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga cgaatcgcct
    20101 gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt tctttccggt
    20161 aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg tatcgctcag
    20221 gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa actgaaaggc
    20281 cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg cgatgttgca
    20341 gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc gaaatttgac
    20401 ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga ctatgttaac
    20461 gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag cttcgttgca
    20521 atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac cctttcctac
    20581 cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa agcgtctctg
    20641 gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc
    20701 atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt ccgcaaaatg
    20761 ctggctcatt gcgaagccgt taccccgatt cgccgtaccg ttactattga agatgtgggt
    20821 aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga agtagtccac
    20881 gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata atcgttctgt
    20941 tggtaaagat gggcggcgtt ctgccgcccg cgttatctct gttatacctt tctgatattt
    21001 gttatcgccg atccgtcttt ctccccttcc cgccttgcgt caggataacg atttccttta
    21061 cgaccaagga gcgcccatgg aacaacgcca catcaccggc aaaagccact ggtatcatga
    21121 aacgcaatcc agtactacgg agtatgacgt tctgcctctg gtcccggaag ccgcaaaggt
    21181 cagcgatccc tttctgctcg acgtgatcct tgatgaagag acgctggccc ccttcctttc
    21241 atggctggtc cctgcgcgcg ttcttgcagt ggagttgttc cctgaccagc ttaccgtgac
    21301 ccgttcacaa accttcaccg cttatgaacg cttgtcgacg gccctgacgg ttgctcaggt
    21361 ttgcggcgtc cagcggttat gtaactacta ttcggcgcga cttacgccgc tccccggacc
    21421 tgattcctcc agggaaaata atcatcggtt ggcacaaatc acgcaatatg cccgccaact
    21481 ggctagctcg cctgctatta tcgacaaccg atcgcgccag catctgaatg acgtcggtct
    21541 tactgtctgg gactgtgtga tcattaacca aatcattggt tttattggct ttcaggcgcg
    21601 gacaattgcg acatttcagg cttatctcgg gcatccggta cgctggttac ccgggctggc
    21661 gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa tcaatacgct ggcgaagcag
    21721 ctatgaggtg gaaaaactac ctgaagagca cacaaaaagt tcaactgcag aactttgcca
    21781 actggccgaa acacactctc tccaccctat ttcactttcc cttctcgaaa agttgttaaa
    21841 cagcacacgg gtcaatacac agccggataa tcagcttgcg gcgttgttat gcgcgcgtat
    21901 aaatggcagt cctgcttgtt tttccacctg tatggattca tcaaatgaat ataaaaaaat
    21961 cagcaccctt ctgcgcaagg gcgaaaatga aattaaccga tgggctgacc gtcattctgt
    22021 tgagcgcgct accgttcagg cgatacaatg gctgacccga gcacccgatc gctttagcgc
    22081 cgcccagttc agccctttac tcgaacacga aaaatcatca acgcagatta ttaatctgct
    22141 ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg gtgagacata
    22201 ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca gccgctctaa tggccaccaa
    22261 aatagacaat tatgtttcag gacaacccgc tgctagcgca gcttaaacag caactgcatt
    22321 cccagacgcc acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt ggcttcctgg
    22381 aagtcgacgc acaaaaaagt tatttcattc cgccgccgca gatgaaaaaa gtcatgcatg
    22441 gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg tgaatccgca gagccagaag
    22501 aactggttga accgttcctg actcgtttcg tgggtaaagt tcagggcaaa aatgaccgtc
    22561 tgaccatcgt tcctgatcat ccacttttaa aagacgccat tccttgccgc gcagcccgtg
    22621 gcctgaacca cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc cgtcatccgc
    22681 tgaaaggcga tcgttctttc tatgctgaac tgacacaata catcactttt ggcgacgacc
    22741 actttgtacc gtggtgggtt acccttgcgc gccataatct ggaaaaagaa gcaccagacg
    22801 gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga agatctgacc tcgctggatt
    22861 ttgtcaccat cgacagtgcc agcactgaag atatggatga cgcccttttc gctaaggcgc
    22921 tgccggatga caaacttcag ctgattgtgg cgattgccga tccaaccgcg tggattgctg
    22981 aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat ctgcctggct
    23041 tcaacatccc tatgctgcct cgcgagcttt ctgacgatct ctgctcactg cgcgccaatg
    23101 aagtccgccc ggtactggca tgccgcatga cgctctccgc tgatggcacc attgaagata
    23161 atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat gaccaggttt
    23221 ctgactggct ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt gccgaacaag
    23281 tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac cacgcactgg
    23341 tgtttaaaga tcgcccggat taccgcttta ttctcggtga aaaaggtgaa gtgctggata
    23401 tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg attgccgcta
    23461 acatttgtgc ggcccgcgta ctgcgcgata agctcggttt tggcatctat aacgtgcata
    23521 tgggctttga tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg cacggtctgc
    23581 atgtcgatgc cgaagaagta ctcacgctgg acggtttctg caaactgcgt cgtgaactgg
    23641 atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg cttccagtca tttgctgaaa
    23701 ttagcactga acccggtcct cactttggcc tcggtctgga agcatacgcc acctggactt
    23761 cgccgatccg taaatatggc gacatgatca accaccgtct gctgaaagcg gttatcaaag
    23821 gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca aatggccgaa cgtcgccgtc
    23881 tcaaccggat ggcagaacgt gatgttggcg actggttata cgcacgcttc ctgaaagata
    23941 aagccgggac cggcacccgt ttcgcggcgg aaattgtcga tatcagccgt ggcggcatgc
    24001 gtgtgcgttt ggttgataac ggcgctatcg cctttattcc ggcacctttc ttacacgctg
    24061 tgcgcgatga actggtttgc agccaggaaa acggcactgt acaaattaaa ggtgaaacgg
    24121 tttacaaagt aactgacgtt attgacgtca ccattgccga agtccgcatg gaaacccgca
    24181 gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ccattcctca tggatgggcc
    24241 gtttatttcc ccgctctacc ttcgttatat ccaggcagat tttttaactg actttcgttt
    24301 gaaaactggc gtttttccaa aactggatta caaataaata tataaaaaac aatgcgttta
    24361 tccgccgtta atttcatgta agtcatgttg ttccttttat ttaaccgcgg actccgctgt
    24421 taaccggagg atatgcatca tgaaaaccgt tagggagtcc acaacgttgt acaactttct
    24481 cggatcgcac aatccatact ggcggttgac ggaaagcagc gatgctttgc gcttttctac
    24541 caccaaaacc acagaacctg atcgtacatt gcagttatct gccgaacagg ctgctcgcat
    24601 cagggaaatg acggtcatca cctccagcct gatgatgagt ctgaccgtcg atgaaagcga
    24661 tctttctgtg catctggtag gacgaaaaat caataaacgg gaatgggctg gcaacgcgtc
    24721 tgcatggcat gacacaccgg cggttgctcg tgatttatca cacgggcttt cctttgctga
    24781 gcaggtagtt tctgaagcac attccgcaat agtgattctc gacagccggg ggaatatcca
    24841 acgcttcaat cggttatgtg aagattacac agggttgaaa gaacacgacg tcattgggca
    24901 aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg gcatccaggc gcaataaccg
    24961 tgtatttttt cgaagcggca atgcatatga agtcgaactg tggataccaa cacgtaaagg
    25021 ccagcggctg tttctgtttc gcaataaatt tgtccacagc ggcagtggca aaaacgagat
    25081 ttttttaatc tgttccggta ccgacattac cgaagagcgc cgcactcagg agcgactgcg
    25141 tattctggca aataccgaca gtatcaccgg actgccgaat cgtaacgcaa tgcaggagtt
    25201 aatcgatcac gctattaatc aggcagataa caataaagtt ggggttgtgt atcttgattt
    25261 ggataatttt aaaaaggtca acgacgccta tgggcatttg tttggtgacc agttattacg
    25321 cgacgtgtca ttggctattt taagctgtct cgaacatgac caggtgttgg cgcgtccagg
    25381 tggcgatgag tttctggtac tggcatccaa tacctcacaa agcgcgctgg aagcaatggc
    25441 atcacgaatt ttgacccgct tacgcctccc ctttcgtatt ggtttaattg aagtttatac
    25501 cagctgttca gtaggtattt cgctctctcc cgaacatggt tcggacagcg cgactattat
    25561 tcgtcacgcc gacacagcaa tgtacacagc gaaggaaggc ggacgaggac aattttgcgt
    25621 ttttactcca gaaatgaatc agcgggtatt tgaatatctc tggctggata ccaacttgcg
    25681 taaagcactg gaaaacgatc agttggttat cgaaaatcac ctagcgtggc gaagtgcgca
    25741 gtctggaagc actagtacgt tggcagtcac ctgaacgtgg gttgattcca ccgttggact
    25801 tcatttccta cgccgaagag tcagggctaa ttgtgccttt aggccgttgg gtgattctcg
    25861 atgtcgtacg ccaggtggca aagtggcggg ataaaggcat aaacctgcga gtggcggtaa
    25921 atatttctgc acgtcagctc gccgatcaaa ccattttcac cgccctgaaa caggttctcc
    25981 aggaactcaa ttttgaatac tgccctatag atgttgaact gacagagagt tgtctgattg
    26041 agaatgatga actggcactg tctgttattc aacaatttag ccaactaggt gcgcaagtgc
    26101 atctggatga ttttggtacc ggctactctt cactttcgca actggcgcgc tttccgatcg
    26161 atgccatcaa acttgaccag gtttttgttc gagatattca caaacaacct gtctcgcagt
    26221 cactggtccg ggcgatcgtc gctgtggccc aggcattgaa tcttcaggtg atcgccgaag
    26281 gtgtagagag tgcaaaggaa gatgcttttt taaccaagaa cgggatcaat gagcggcaag
    26341 gatttttgtt tgccaaaccg atgcccgccg tcgccttcga acgctggtat aaacgctatc
    26401 tgaagcgcgc ataagtagat gaatatatat acttagcgct atatcccgtc attatatatc
    26461 gtattcctgg atgataatta gccgttgctg ttaaacactt ctcttttagc ggcaattttt
    26521 gcattattca ttttccccct tttgctataa ggatgtcctt gatgtctgaa tttgatgccc
    26581 agcgcgttgc cgaacgtatt gatattgtgt tagatatcct tgtggcaggc gattaccact
    26641 ctgcgatcca taatctcgag atacttaaag cagaactctt gcgccaggtt gctgaatcta
    26701 cgccagacat cccgaagaca ccgtgggaga tttaacaatc gtgccttcag gttttcctat
    26761 ttgaggatgc ccggcgcgtc agtatttttt tatttagtat tataacgtta taagaattac
    26821 agcgatgaat tcccgacaac aaactattct acagatggtc attgaccagg gtcaggttag
    26881 cgtaaccgat ctggcaaaag ccactggagt ttctgaagtt accattcgcc aggatctcaa
    26941 caccctcgaa aaactgagtt acctccgccg tgcacatggc tttgcagttt cgcttgatag
    27001 tgatgacgtc gaaacccgta tgatgagcaa ctatacgctg aagcgtgaac tcgccgagtt
    27061 tgccgcgtca ctggttcaac cgggcgaaac catctttatc gaaaatggca gcagcaatgc
    27121 cctgctcgcc cggactctgg gggagcagaa gaaaaatgtc actatcatca cggtcagcag
    27181 ctacatcgcg catttgctga aagacgcgcc ttgtgaagtc attttgctcg gtggcgtgta
    27241 ccagaaaaaa agcgaaagta tggttggccc tttgacacgc cagtgcatcc agcaggtaca
    27301 tttcagcaaa gcatttattg gtattgatgg ctggcaacct gaaactggat tcaccggtcg
    27361 cgatatgatg cgcaccgatg tggtcaatgc cgtgctggaa aaa
//
LOCUS       DAJTXY010000041        27299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTXY010000041 DAJTXY010000000
VERSION     DAJTXY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.041"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(99..389)
                     /gene="ubiK"
                     /locus_tag="OO566_001891"
     CDS             complement(99..389)
                     /gene="ubiK"
                     /locus_tag="OO566_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6605195.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
     regulatory      511..659
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            763..1416
                     /gene="ribB"
                     /locus_tag="OO566_001892"
     CDS             763..1416
                     /gene="ribB"
                     /locus_tag="OO566_001892"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6605196.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1677..1847
                     /locus_tag="OO566_001893"
     CDS             1677..1847
                     /locus_tag="OO566_001893"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605197.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1905..2678)
                     /gene="zupT"
                     /locus_tag="OO566_001894"
     CDS             complement(1905..2678)
                     /gene="zupT"
                     /locus_tag="OO566_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6605198.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2821..3609
                     /gene="ygiD"
                     /locus_tag="OO566_001895"
     CDS             2821..3609
                     /gene="ygiD"
                     /locus_tag="OO566_001895"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6605199.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3647..4807)
                     /locus_tag="OO566_001896"
     CDS             complement(3647..4807)
                     /locus_tag="OO566_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6605200.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(4813..5484)
                     /locus_tag="OO566_001897"
     CDS             complement(4813..5484)
                     /locus_tag="OO566_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6605201.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5632..7113)
                     /gene="tolC"
                     /locus_tag="OO566_001898"
     CDS             complement(5632..7113)
                     /gene="tolC"
                     /locus_tag="OO566_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6605202.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQACLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7318..7947
                     /gene="nudF"
                     /locus_tag="OO566_001899"
     CDS             7318..7947
                     /gene="nudF"
                     /locus_tag="OO566_001899"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6605203.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7948..8370
                     /locus_tag="OO566_001900"
                     /pseudo
     CDS             7948..8370
                     /locus_tag="OO566_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
     gene            8395..9222
                     /gene="cpdA"
                     /locus_tag="OO566_001901"
     CDS             8395..9222
                     /gene="cpdA"
                     /locus_tag="OO566_001901"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6605204.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9222..9803
                     /gene="yqiA"
                     /locus_tag="OO566_001902"
     CDS             9222..9803
                     /gene="yqiA"
                     /locus_tag="OO566_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6605205.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9832..11724
                     /gene="parE"
                     /locus_tag="OO566_001903"
     CDS             9832..11724
                     /gene="parE"
                     /locus_tag="OO566_001903"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6605206.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11772..12086)
                     /locus_tag="OO566_001904"
     CDS             complement(11772..12086)
                     /locus_tag="OO566_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6605207.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12117..12698)
                     /gene="mdaB"
                     /locus_tag="OO566_001905"
     CDS             complement(12117..12698)
                     /gene="mdaB"
                     /locus_tag="OO566_001905"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6605208.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            13017..13349
                     /locus_tag="OO566_001906"
     CDS             13017..13349
                     /locus_tag="OO566_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6605209.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(13395..14744)
                     /gene="qseC"
                     /locus_tag="OO566_001907"
     CDS             complement(13395..14744)
                     /gene="qseC"
                     /locus_tag="OO566_001907"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6605210.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14741..15400)
                     /gene="qseB"
                     /locus_tag="OO566_001908"
     CDS             complement(14741..15400)
                     /gene="qseB"
                     /locus_tag="OO566_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6605211.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15552..15944
                     /gene="ygiW"
                     /locus_tag="OO566_001909"
     CDS             15552..15944
                     /gene="ygiW"
                     /locus_tag="OO566_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6605212.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15997..16479
                     /locus_tag="OO566_001910"
     CDS             15997..16479
                     /locus_tag="OO566_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6605213.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            17025..19283
                     /gene="parC"
                     /locus_tag="OO566_001911"
     CDS             17025..19283
                     /gene="parC"
                     /locus_tag="OO566_001911"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6605214.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19517..20254
                     /gene="plsC"
                     /locus_tag="OO566_001912"
                     /gene_synonym="parF"
     CDS             19517..20254
                     /gene="plsC"
                     /locus_tag="OO566_001912"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6605215.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20329..21741
                     /gene="ftsP"
                     /locus_tag="OO566_001913"
     CDS             20329..21741
                     /gene="ftsP"
                     /locus_tag="OO566_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6605216.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21852..24069
                     /locus_tag="OO566_001914"
                     /pseudo
     CDS             21852..24069
                     /locus_tag="OO566_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
     gene            complement(24112..24369)
                     /gene="yqhH"
                     /locus_tag="OO566_001915"
     CDS             complement(24112..24369)
                     /gene="yqhH"
                     /locus_tag="OO566_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6605217.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24420..25346)
                     /locus_tag="OO566_001916"
     CDS             complement(24420..25346)
                     /locus_tag="OO566_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6605218.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNNNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            complement(25546..26373)
                     /gene="dkgA"
                     /locus_tag="OO566_001917"
     CDS             complement(25546..26373)
                     /gene="dkgA"
                     /locus_tag="OO566_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6605219.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            complement(26478..>27299)
                     /locus_tag="OO566_001918"
     CDS             complement(26478..>27299)
                     /locus_tag="OO566_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR6605220.1"
                     /translation="DPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTG
                     DKQAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRF
                     AEGILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELT
                     AMHGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRN
                     FFEQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKAAR"
BASE COUNT         6385 a         6773 c         7270 g         6871 t
ORIGIN      
        1 tgtgatgata ctctgaatgg gattaagcga taatgtaata gaaaacactg taaattagag
       61 gattaattca taatttacag tgcattaaag acaaatgttt acagcgttgg ggggagagtc
      121 tctggatctg gctgcttttt gatctcagta ctgcgatttt ccagttcgct gattctttgt
      181 tcaagcagcg ccagtttctc acgagtccgt aacaggactt gcgtttggac gtcgaactct
      241 tcacggctta caaggtcgag gcgcgtcagc tgagcttgta gggtttggcg gatttttttc
      301 tccacatctt ccccgaactc cctgattcct ttaggcattg attcgtgaac ctggcgagcg
      361 atttgctcaa tttttttcgg gtcaatcatt gtagtttccc tgtactgata ggtgttgagt
      421 accattgtag tgcgataagg gtaagtcata aaccagaatt atgtgaagct atgcgttgct
      481 gccgctaatc attagagtta tagtgaatcc gcttattctc agggcggggc gaaattcccc
      541 accggcggta aatcaactca gttgaaagcc cgcgagcgct ttgggtgcga actcaaagga
      601 cagcagatcc ggtgtaattc cggggccgac ggttagagtc cggatgggag agagtaacga
      661 ttctgtcggg catggacccg ctcacgttat tttggctata tgccgccact cctaagactg
      721 ccctgattct ggtaaccata attttagtga ggttttttta ccatgaatca gacgctactt
      781 tcctcttttg gtacgccttt cgaacgtgtt gaaaatgcac tggctgcact gcgtgaagga
      841 cgcggtgtaa tggtgcttga tgatgaagac cgtgaaaacg aaggcgatat gatcttcccg
      901 gcagaaacca tgaccgttga gcagatggcg ctgaccattc gccacggtag cggtattgtt
      961 tgcctgtgca tcactgaaga tcgccgtaaa caacttgatc tgccaatgat ggtagaaaat
     1021 aacaccagcg cctatggcac cggttttacc gtgaccattg aagcggctga aggtgtgact
     1081 accggtgttt ctgccgctga tcgtattacg accgttcgcg cagcgattgc cgatggcgca
     1141 aaaccttctg atctgaatcg cccgggccac gttttcccac ttcgcgcgca ggcaggtggt
     1201 gtactgacgc gtggcggtca tactgaagcg actattgatt tgatgacgct ggcaggcttt
     1261 aaaccggctg gtgtgctgtg tgagctgact aatgacgatg gcacgatggc gcgtgcacca
     1321 gagtgcattg agtttgccaa taaacacaat atggcgctcg tgactattga agacctggtg
     1381 gcataccgtc aggcacatga gcgtaaagcc agctgaaaac cgctgcttaa ttactgcctt
     1441 aatcaagaaa ccgaagttgt agctggcttc ggtttttatt ttgccctgct atgcaagatg
     1501 ttaattccgc ttctctttct atgagaaaat ttcattaata tcaggcattc tttttcatta
     1561 tgatcaaaat catataattg ttccgttatt atgcgaccat atgaatatat ccgaaaaagg
     1621 atatgtgaaa tttatgcgct aaatgtttag tattgttaaa tattaagggt aaaaggatgt
     1681 ttatcgcctg gtactggatt gtattgattg ctctggttgt ggtgggttat ttcctgcatt
     1741 tgaaacgtta ttgtcgggcg tttcgccagg acagagacgc actgcttgaa gcgcggaaca
     1801 aatacttaaa cagtacgaga gaagagacag ccgaaaaggt agaataaggc tttcgtgaag
     1861 ctggcagaag ttgtccatgt gctggacaac ttctgatgtt gtttttaacc aattcccgcc
     1921 gtttgtagca gcactaaact gaatcccatc actgacattc cacatagtac gccatagctg
     1981 gggttattat tagggtcaat ctctttggcg agcggcatta attcatcaac cgagagcgcc
     2041 accataattc ctgcaaccgc cgccatgatt gccgccatga ccaccgggga aatcatgcta
     2101 ccgaggatta accacgccag cacaccacca agaatttctg ctagtccaga aatccccgcc
     2161 cacagaatcg cggtgcgttt agaccccgtt gccgcataaa ccgggcctgc cactgccaga
     2221 ccttcaggga tattgtgcaa cgcgacggcc agtgcgatgc caaatcccag ctccaggttg
     2281 ctgctcgccg tgacaaaggt ggcaatccct tccgggaagt tatgcagact gatgccgaga
     2341 gtgagcagaa tggctgtgcg cttgatcgat tttggcaaca gctgcaccga tttttgcatt
     2401 aaatcctgcg gatgcgcatg cggcaacatg cggtccaggc caaagtagcc aagcagaccg
     2461 aagataaaca ttccataacc caacaccggc gacattcctt cagctgctag tgcggcagga
     2521 agcatttcca ttaatgagat gagcaacatg atccccgccg caaaacctag cgaaaacgcc
     2581 agtaagcggt tcgagggttt ttgtccgaga acgccgagaa acgcgccaat aaacgtggct
     2641 gcccccgcca gtatggtcag aatgagaggt actgacatcg acaactcctt ataaatcttc
     2701 accttcaggc agatcgtcaa aataactgat gatcatcatc attgttatcc gggttcttac
     2761 tccgccgaac aagcgtattg tgaggatatc aaaatgacac ctttagttaa ggatatcatc
     2821 atgtcttcaa cacgtatgcc agcattgttt ttaggtcacg gtagtccgat gaacgtgctg
     2881 gaagataatt tgtatacccg cagctggcag aagttgggga tgacattgcc acgcccgcaa
     2941 gcgattgtgg tggtttcggc tcactggttt acccgtggaa caggagtgac cgcgatggag
     3001 acgccgccca cgattcatga ctttggtggc ttcccgcagg cgctgtacga tacgcattat
     3061 cctgctccgg gttcgcctgc gctggcacag cgtctggttg agctgttagc gccgatcccg
     3121 gtgacgctgg ataaagaagc ctggggcttt gaccacggct cgtggggcgt gctgattaag
     3181 atgtatcctg acgccgatat ccctatggtg cagttgagta tcgacagtag caaacctgcc
     3241 gcctggcatt tcgaaatggg gcgcaaactc gcggcgttgc gtgatgaagg aataatgttg
     3301 gtcgccagcg gtaacgtggt gcataacctg cgcacagtga agtggcacgg tgatagttca
     3361 ccgtatccgt gggcgacgtc gtttaatgag tatgtgaaag cgaatctgac gtggcaaggg
     3421 ccggtagagc agcatcctct ggtgaattat ctcgaccatg aaggtggcgc gttatcgaac
     3481 ccaacgccag agcactatct gccgttgttg tatgtgttag gtgtgtggga tgggcaggag
     3541 ccaattacca ttccggtcga tggtatagaa atgggcagtt taagtatgct gtcggtgcag
     3601 ataggctaac aattaccgga tggcgagtgc catccggtat cgtggcttat tcaacaaaaa
     3661 tatgtggata aaaccgagac atatcctggg tgatcaacgc acggtcttca cgaatgccaa
     3721 ttccggcagg ttgatcgttc accagccagc taccaatcag catatagctg tcgccgaatt
     3781 tcggtaacgg gtggaattgc tgaacaatca tcccttcttc gccatacgga ccttccgctg
     3841 cttcaatggt tttgccgttc tcaatgatcg acacgtttgc gccttcacgg gagaagatcg
     3901 gtttaaccac atatttttcc atttgcggat gatcatcttc cgcaaaataa gcgggcagca
     3961 ggttcgggtg attcgggaac atctcccaca gtagcggtag aagtgccttg ttggagataa
     4021 tactcttcca cgccggttcc agccagcgta cgccagcatc ttccagcttg gtggagaaca
     4081 tttcgcgcag cataaattcc cacggataaa gcttgaacag gttagaaatc acctgatcct
     4141 gtaaatccgt gaactggcct ttttcgccta aacctatctc gtcgatgtag aggaactcgg
     4201 tggcgatttc agcttcggtt gcgcagtcct gcaaatactg aatggttccg cgatcttcca
     4261 ccgtgtcgcg acagcaggtg agatgcagca actggaagcc acactgttca cgcagctcaa
     4321 cgaagcgatc gatcagtttt tcttgcagac tgttaaactg gtcgctgccc tccggcaagt
     4381 taccggcgtt aagctgatct tccagccaga tccactgaaa gaacgccgcc tcgtatagtg
     4441 acgttggcgt atcggcgtta ttttccagaa gtttaggttc gccagtgcca tcccacgcca
     4501 gatcaagacg cgaataaagc gatggctggt gagtcagcca tgattgacgc acaaaactcc
     4561 aggtgtgttt tggtatgcgg aatttggtca tcagctcatc gctggcgatc actttttcca
     4621 ccactttcag gcacatctgg tgcagttcgg cggtgacttc ttccagcttt tcaacctggg
     4681 cgagggtcaa cttgtagtaa gcatcttcac accagtacgg ctcgccgtac atggtgtgaa
     4741 aattgaaacc gtattcgtgg gctttctcac gccagtccgg gcgctcggta atactgactc
     4801 tttccatcgg tatcagccac ccattgaacg agaagaggtg ccggttgcgc tacgctgcat
     4861 agtgctttgt ttggcaacag attcaccaaa accgccacgg gtaacggtag tggtggtcgc
     4921 cggttttggt gccattgccg tcttcggtac ggtcatggtg cggcctggct gggctgcgcc
     4981 atagttttta cccgtcgcgt cggtatattt accgtaagcc ggactggccg ggtttttcga
     5041 ggagaacagc ggctgctgtg caaatcccgc gccgccgccc atcagacgcc ccatcatgta
     5101 accggccatc agcggcatcc agaaactccc gctggattgc tgagcctgcg cctggttttc
     5161 tggtgccatg ccagcctggg ctggtgcttg ctgacactga ccttcaccaa attcagcaac
     5221 acagtcttca cgggtggcgt atttcggcgc agtacgttcg gcttctttca gcgcattgtt
     5281 gtacgcggtg gtacattcgg cgcttttgcc tgggtttgca gctgaacagt cgtcagcatt
     5341 ttgatagaga gacactgttt catcactctt ttcacagcca gccagcataa aaacagtggc
     5401 aaccgcgaga gcgactggtg tcagatggcg tgcgctccag tttttgcgga acgatgcgtg
     5461 gcgtatggat tttgtccgtt tcatttttgt cttccgggac cagtggtaaa tacccatcag
     5521 aatagaggat ggctggtcga aattgaagcg agaaggggga agaatgcggc agataacccg
     5581 tatctttacg ttgccttacg ttcagacggg gccgaagccc cgtcgtcgtc atcagttacg
     5641 gaaagggtta tgaccgttac tggtggtagt gcgtgcggat gtttgctgaa cgacgggtgc
     5701 cgggctatca ggcgcataac catcagcaat agcattctgt tccggcgttt gcggggcaac
     5761 gttttccgga ttagtggaaa ccggtttgct cagcgcattg ttcagtgcca gcaaatcctg
     5821 ctcgttcaac gtacccaggg ctgacttaat attcagctga ttaatcaggt agttataacg
     5881 cgcattcgcc agctcttgct tggcgttgta cagcgtggtg gtcgcatcca acacatcaac
     5941 aatggtacgc gtaccgaccg agtagcccgc ttccatcgcg tctaatgagc tttgcgcgga
     6001 aactacggct tgtttgtagg cgttaatact actgatagaa gcattaatgt tgttgaagga
     6061 ggaacgtacg gtttgcacga cgctacgatg tgcgctttcc agttgctcac tggcaccaac
     6121 aaagttgtac tgtgcctgtt tcacctgcga gttaaccatt ccgccctgat aaatcggcag
     6181 cgagaagctc aggccaactt tgttctggcc catattgcta tcgtcatact gggtaccagc
     6241 ggcaccacgg gttttcgaac cgctataaga ggtgtcagaa atcccggtag aagccgttaa
     6301 atccagagtc ggtaagtgac catcctgcgc ctggcgaatt tgctcgcgcg ccaggtcctg
     6361 gctcaagcat gcctgtaaca gcgacaggtt gcgtttttcg gcttctttca gcagcgcgtt
     6421 aaccggctgt ggtttgtcgg ttttaaagtt ttcgacattc agcgccgcca gttccggata
     6481 gtagttaccg gtgatctggc gcagctgctc taccgcgtta tcaaggttat tacgtgcggt
     6541 cacttcgttc gccagcacgg tatcgtactg cgcgcgggcg ttctgcacgt cggtgatcgc
     6601 taccaggccc acgttaaaac gttgggtggt ttgatctaat tgacggtaga tcgcttcttt
     6661 ttgtgcctgt gtataggaaa gaacgtcaat agcattcaac acgttgaaat aagcggtcgc
     6721 ggtgttgagg atcaaggttt gctgatcggt ctgatatgtg acgtcctgaa tccctgctgc
     6781 tttttcctgc agcgttaacg cacgccattt cgacatatca aaaatggatt gagttaactg
     6841 cagggacgca ctggtcgcgt tcgagttgat gccgttcgcg tcgcggtagc cgttgctata
     6901 ggtgtaatct gcacctaaac ctagctgtgg cagtaatgga ctgcgcgctt cattaatttt
     6961 ttcaaaggca gcatcacgat cggcggcaga cttacgcaat tccgggttac taaggcgtgc
     7021 ttgctgataa acttgcatca ggttctcggc ctggctcaac gaactgaacc cagaaaggct
     7081 caggccgata agaatgggga gcaatttctt catttgcatt ccttgtggtg aagcagtatt
     7141 tagcgcgatc aaactgtaaa attattgcgg attctagcag aagccgctac cgcaaaaggt
     7201 tggcgttacg tgccataggg cgttaatgtg cctcaattta acatatgact gctcaaacgg
     7261 cagtcaaacg tcgctgaaat tcacatttaa ttcactatta gtgccaggac attaacaatg
     7321 cttaagccag acaacctgcc cgttacattt ggcaaaaacg atgtagaaat tattgcacga
     7381 gaaacacttt atcgcggctt tttttcatta gatctttata gatttcgtca tcgtctattc
     7441 aacgggcaaa tgagtcatga ggtacggcgg gaaatttttg agcgcggtca cgccgcagtc
     7501 ttgctaccct ttgacccagt gcgtgatgaa gttgtgctga ttgagcagat tcggattgcc
     7561 gcgtacgaca ccagcgaaac cccctggcta ctggagatgg ttgccgggat gattgaagag
     7621 ggtgaaagtg tggaagatgt tgcccgtcgc gaagcgattg aagaggcggg actgatagtc
     7681 aaacggacca aaccggtgtt aagtttcctg gcaagcccgg ggggcaccag tgagcgttcg
     7741 tcaattatgg tgggcgaagt ggacgccacg accgcaagcg gtattcatgg tctggctgat
     7801 gaaaacgaag atattcgcgt tcatgtggta agccgggaac aggcatacca gtgggtagaa
     7861 gaggggaaaa tcgacaacgc agcgtcggtc atcgctttgc aatggctgca gctgcatcat
     7921 caagcgttaa aaaatgagtg ggcataaatg aagcgttaca cacctgactt tcctgaaatg
     7981 atgcgcctgt gcgagatgaa cttttcataa ttgcgccgtt tgttaccgcg caattacgca
     8041 cccggcgaaa ctgtaagcta tcaggtggca aacgcacaat atcggctgac gattgtggaa
     8101 tcgacccgat acactaccct ggtgacaata gaacagactg cgcccgcgat cagttactgg
     8161 agccttccgt caatgacggt gcgtctgtat catgacgcga tggtggctga agtgtgttca
     8221 agccagcaga tttttcgctt caaagcgcgg tatgattatc ctaataaaaa gttgcatcaa
     8281 cgcgacgaaa agcatcaaat taaccagttt ttagcggact ggttgcgata ctgtttagca
     8341 catggagcga tggcgattcc ggtttactag cgtcgtgaaa cctaaggaca ccatttggaa
     8401 agcctgttaa cccttcctct ggctggtgag gccagaggaa ggattttaca aattaccgat
     8461 actcacctgt ttgcacaaaa gcacgaagcc ctgttagggg taaacacctg ggaaagctac
     8521 caggcggtgc tggaggcgat tcgtccacac cagcacgaat tcgacctgat tgtcgcgaca
     8581 ggtgatttag cgcaggatca atcctctgcg gcctatcagc atttcgctga aggcatcgca
     8641 agttttcgtg cgccctgcgt ctggctgccg ggcaaccacg atttccagcc cgcgatgtac
     8701 agcgcgttac aggatgcggg tatctccccg gcgaagcggg tgtttattgg tgagcaatgg
     8761 caaatcctgt tgctggatag ccaggtgttt ggcgtgccgc acggtgagct gagcgagttt
     8821 cagcttgagt ggctggaacg taaactggcc gatgcgccag aacgccatac gttgctgctg
     8881 ctgcatcatc atccgctacc tgcgggttgt agttggctcg atcaacacag tctgcgtaac
     8941 gcgggcgaac tggataccgt gctggcgaag tttccgcacg tcaaatactt gctgtgcggt
     9001 catattcatc aggagctgga tctcgactgg aacggtcgcc gcctgctggc aacgccgtcg
     9061 acctgtgtgc agtttaagcc gcactgttcc aactttacgc tggatactat cgcgcccggc
     9121 tggcgtactc tcgagttaca tgctgatggc acgctgacca ccgaggtgca tcgcctagcg
     9181 gacacacgtt tccaacctga taccgcttca gaaggctact gatgtctacg cttctttatt
     9241 tacacggttt caacagctcg ccgcgctctg caaaagcgag cttgttaaaa aactggctgg
     9301 cggaacatca ccctgacgtt gagatgatca ttccgcagtt gccgccgtat ccttccgacg
     9361 cggcagaact gctggaatcc attgtcctgg aacatggcgg cgattcgctg ggtattgtcg
     9421 gttcgtcact ggggggatat tacgccacct ggttgtcgca atgttttatg ctgcccgcag
     9481 tggtggtaaa cccggcggta cgcccgtttg aattgctgac ggactatctc ggtcagaacg
     9541 agaaccccta caccgggcag caatatgtgc tagagtcacg ccatatttac gatctcaaag
     9601 tcatgcagat tgacccgctg gaagcgccgg atttgatctg gctgctgcaa cagacgggag
     9661 atgaagtgct ggattaccgc caggcggtgg cgtactacgc ttcctgccgc cagactgtca
     9721 tagaaggcgg caaccacgca ttcacgggct tcgaagatta tttcaacccg atcgtcgatt
     9781 ttcttggtct gcaccatctc tgacgatcaa ctcgaattgc taacttaaac catgacgcaa
     9841 acttataacg ctgatgccat tgaggtactc accgggcttg agccggttcg ccgccgtccg
     9901 gggatgtata ccgataccac tcgccctaac catttggggc aagaagttat tgataacagt
     9961 gtcgatgaag cactggcggg tcacgcaaaa cgcgtggatg taatcttaca tgctgaccag
    10021 tcgttagaag tgattgacga tgggcgcggg atgccggtgg atattcaccc ggaagaagga
    10081 gtaccggcgg ttgaactgat tctttgccgt ctgcatgcag gcggtaaatt ctctaacaaa
    10141 aattaccagt tctctggcgg cctgcatggc gtggggattt cggtagttaa cgccttgtcg
    10201 aagcgcgtag aagttaacgt gcgccgcgat ggtcaggttt ataacatcgc ctttgaaaat
    10261 ggcgaaaagg tgcaggattt acaggttgtc ggcacttgcg gtaaacgcaa taccggtacc
    10321 agcgtgcact tctggccgga tgaaaccttc tttgacagcc cgcgtttttc tgtttcacgc
    10381 ctgacgcatg tgctgaaggc caaagcagta ctgtgccccg gcgttgagat tacctttaaa
    10441 gatgagatca acaacaccga acagcgctgg tgctatcagg acggtctgaa tgattacctg
    10501 gcggaagcgg taaacggttt accgacgctg ccagaaaaac cgtttatcgg taatttcgct
    10561 ggcgatactg aagctgtgga ctgggcgcta ctgtggctgc cggaaggcgg tgaactgctg
    10621 accgaaagct acgtcaacct tatcccaacg atgcagggcg gtacccatgt taatggtctg
    10681 cgtcagggcc tgttggacgc gatgcgtgag ttctgtgaat accgcaacat tctgccgcgc
    10741 ggcgtgaagc tgtcggcgga agatatctgg gatcgctgcg cctatgttct gtcggttaaa
    10801 atgcaggatc cgcagtttgc cgggcagacc aaagagcgtc tctcttcgcg tcagtgtgcg
    10861 gcattcgttt cgggcgtggt gaaagatgcc ttcatcctgt ggctgaacca gaacgttcag
    10921 gcggcggagt tgctggcaga gatggcgatt tccagcgccc agcgtcgtat gcgtgcggct
    10981 aaaaaagtgg tgcgtaaaaa gctgaccagc ggcccggcgc tgcctggcaa actggctgat
    11041 tgtaccgcgc aggaccttaa ccgtaccgaa ctgttcctcg tggaaggtga ctccgcaggc
    11101 ggatctgcca agcaggcgcg cgatcgtgaa tatcaggcga tcatgccgct gaaaggtaag
    11161 atccttaaca cctgggaagt ttcttccgac gaagtgctgg cctcgcagga agtgcatgat
    11221 atttcggtag cgatcggtat cgatcctgac agcgacgatc tgagccagct tcgttatggc
    11281 aaaatctgta tcctcgcgga tgcggactct gatggtctgc acattgccac gctgctctgc
    11341 gctttgttcg taaaacactt ccgcgcgttg gtgaaacacg gtcacgttta cgtcgcactg
    11401 ccaccgctct accgtattga tctcgggaaa gaggtttatt acgcgctgac ggaagaagag
    11461 aaagagggcg tacttgagca attaaaacgc aagaaaggca agccgaacgt ccagcgtttt
    11521 aaaggtctgg gggaaatgaa cccgatgcaa ttgcgtgaaa ccacgcttga tccgaacact
    11581 cgccgtctgg tgcagttgac tatcgatgat gaagacgatc agcgtactga cgcgatgatg
    11641 gatatgctgc tggcgaagaa acgctcggaa gatcgccgca actggttgca agagaaaggc
    11701 gacatggcgg agattgaggt ttaaaggaaa gaacattgcc cggtcaggat ggccgggcaa
    11761 acaaggcagg attaaatccc tggctgcaga atacggatat tcatctccag cacgtcgcct
    11821 tttacggctt tgctgtacgc cttcatgtgc ggggtttgca gatgcgcttc aaggtgcgcg
    11881 atgctttccc actgctcaat catcacgata gaatccggtg ccatagactg gaaactcacg
    11941 ccagcagcgc aatccaccat tggcgcatag ccgtggcaac cttcttcttt cagtacggtt
    12001 ggaacgattt tagcaaactg atccaatacc gcctgacggt gatgttgacc aggacgagta
    12061 cggatttctg cgattacggt aagcatggtt aactccttct aaagcctgag ctctagttaa
    12121 ccaaaaattt ccacaagatg cttgcgatat tcttcagtat agcggggaac atcaggcatt
    12181 tttatcacgt cattagcgat aaatgtcggc agcggttcca taccgaggaa ttggtttgct
    12241 ttatggaacg gcagatacac accgtcaacg ccaacgccgt ggaagaactg atctttttcg
    12301 gtgaaggctt ccattggtgc gttccaggtt agagaaagca tatatttttt gccctgtacc
    12361 aggccgccgg aaccgtattt tttcgacgga tctttgcggg tacgaccatc gctggcatac
    12421 agagtcccgt gaccttcggt gaatacatca tcaatatatt ttttcactgt ccacggcgcg
    12481 cccatccacc agcctggcat ctgccagatc accacatcag cccagagaaa gttttgtact
    12541 tccgctttga cattgtagtc gctgtcggcg cgaacgatgc ggacatcatg cccgaggtcg
    12601 cgcagtgtgc catccgcgac ttcggtcagg gtgtcgttca gttgaccatt ggagtgggcg
    12661 aattttttcg cgccgttgat aatcaggatg ttgctcattt tttaacctca aggtgagacg
    12721 tttgtcgact atagtacgtc acagagcggt acggtgaaat tagcaaaatg tgcaaagtct
    12781 tttgcgaatt tagcaaaagt ctgatctagc tctgactcac cgatgcagga taaatatttt
    12841 ctgaaaatga caatactgat agccattcct cataattctt actcgtaccg gaataattta
    12901 cacgtttggt tcggtttttt tggggattga aaatatggct ttgaataagt aaaagatgaa
    12961 aatatcaacg tgtcatgttg ataattataa atggattttt atgagcgtac tgatatatgt
    13021 taaagcagaa aattaaaaca atatttgaag cattgctata tataatgctt acttattggt
    13081 taatagattc tttttttgct ttcaataagt atgactggat gcttgagtcg ggaggcaata
    13141 tttgcagtat accttccgtt tcaggcgaag atcgtatatt gcaagcaatg attgcagcat
    13201 tttttcttct tacgccactt attattctta tcttaagaaa actctttatg cgggaaatgt
    13261 tcgagttttg gctatatgta ttctcgctcg ttatttgtct ggtttgtggt tggtggcttt
    13321 tttggggacg gtttatattt tgctattagt aaaatctatg agagtcgttt taacggctct
    13381 catagacaga gaagttacca gcttaccttc gcctcaaatc caccttgttc cgcattccca
    13441 aactcaacat tcatgtcatg cagcttggcg attcgctgga caatcgatag cccaagtcca
    13501 ctgccggtag cggtttgtcc gggtgggcga tagaagcgtt cgccaattcg cgccagtgcc
    13561 tctggtgtca caccggggcc gttatccctc acgatgaaat tatcagcatt cagcgtgacg
    13621 tctatcacgc tgccctgtgg actgtagcgc acggcgttat ccagcaaatt tcgcaccaac
    13681 aaacttagca atagcggttg tccggtgcgt ttgatgccgt gggcattgag tgtcagtcgc
    13741 acgtcaattt tcgcctgctg cgccgtgtgg taaatatcca tcaccgacga ttgtaggaga
    13801 tcttcaagcg ggatctccgc gacgtcctga aggttatcca gtgagtccag ccgcgatagc
    13861 gtgagcagtt gatcaaccag acgagtagcg cgatcgatcc cggaatgtaa ttggagcagt
    13921 gcttttttcc gcgcctgcgg atcatcgtca gagagctgcg caacttcggt ttgcactttc
    13981 agcgctgtta acgggctacg tagttcgtga gctgcatcgg aggtaaagcg tcgttctcga
    14041 accatcatcg catgtgtgcg ggcgaacagt tgatttagcg actcaaccag tggccgcact
    14101 tcgctgggta cgccagtcgc gtttagtggt ttttccgagt cagggtcacg catacgtagt
    14161 gccagcgcca gtttgttcag cggcgcgagt tcacgaccca gtagtaccat catgatgatt
    14221 aacataatcg gcagtgcgac cagccacggg atcaattgcc cggcaacaat cgccagcgcc
    14281 atgtcttcac ggtattccca ttcctggcca acaacgatgc gatatttgcc atcaggtgag
    14341 gtcatccaga caaaacgcca aggatcgtct tcaccgacca gttgcccgtc agcaaaacct
    14401 tcccgttgat agctataggg aatatcttct ccgttatcgc catcattaag gaccattctg
    14461 ccgtcgtggg taaagatggc aaaggtcagc gcatcgtcat caacatgacc gtgttttaat
    14521 ttatttggcg tctgtgccat gcgatccgcc gcgttgattt cgttgagatc gagcgtactt
    14581 aaccgtttgg caaacagcat cagttgggtg tcgaacaatt catcgacgtt atccgttgtt
    14641 tgtttccagg cgacaaagct ggaaagcagc caggtcaccg aggccagaat taaaaagatt
    14701 agcgtcagcc tgacgcgcag actaagacgt tgggtaaatt tcatttctca cctaatgtgt
    14761 agccaatacc atgcacggta cgaataaaat cactaccgag tttgcgtcgc agatgatgca
    14821 catgcacttc aacggcatta ctggtgacct cttcgtccca ggtatacagt ttctcttcaa
    14881 tcagtttgcg cggcagtacc cgaccagcgt tacgcatcag taattccagc agggcaaatt
    14941 cttttggttt cagtgttaag ggttcgccag ccagcgtggc gatacgtttg ccggggtcga
    15001 gcatgacgtt accgtggcgc agctcgttgc tggcctggcc gttggttcgg cgcatcagag
    15061 cctccagcct ggcggcgact tctatcaacg caaaaggttt acacagataa tcgtcagctc
    15121 ccagacgcag cccttctaca cgttccgcta acgcatcacg cgcggtcagg ttcagtaccg
    15181 gctcacgctg acctttctct cgccattcgc gcaaaatatc gcgaccatcc atgcctggta
    15241 aggttaaatc caggatcacc gcatcataag gtgcgctata aagcgcctct tttccctgac
    15301 gaccttgtgt aaaccagtcg acactaaaac ccattttact aaggccagtt ttgatgccgt
    15361 caccaatcag catgtcatct tctatcagta aaattcgcat tttttcatcc ctgcgataac
    15421 cgtatttgct gccagtaaac cgtgttgtaa cgcgctctgt acagcattaa attttatttt
    15481 tttcccttaa gaagttgtta aggactatct tgttaaatgc tcggaaacag acattaaagg
    15541 gagtaataaa catgaaaaaa ttcgcagcag taatcgcagt aatggccctg tgcagtgcac
    15601 cggtgatggc agcagagcag ggcggttttt ctggcccatc ggcaacgcaa agtcaggccg
    15661 gaggatttca ggggccgaac ggcagcgtaa cgactgtaga aagcgtaaaa tccctgcgtg
    15721 acgacacctg ggtaaccctg cgcggcaata tcgttgaacg catctctgac gatctctacg
    15781 tgtttaaaga tgccagcggt actatcaatg ttgatatcga tcacaaacgc tggaacggtg
    15841 tgacggtgac gccgaaagat acggttgaga ttcagggtga agtcgataaa gactggaatt
    15901 ctgttgaaat tgacgtcaaa cagatccgca aagtaaatcc gtaattgtta ccgctcccgg
    15961 gacgcgttcc cgggaataat ttcgcaggga ggcaaaatga caaacctgac actggatgta
    16021 aacattatcg atttcccatc aatccctgtg gcgatgttgc cgcaccgctg tagccctgag
    16081 ttgctcaact acagtgtggc gaaatttatc atgtggcgta aagagacggg gctttctcct
    16141 gttaaccaaa gccagacttt tggcgtcgcc tgggacgacc ctgccaccac cgcaccggaa
    16201 gcgtttcgct ttgatatctg cggcagcgtt agcgaaccga ttcccgataa tcgttatggt
    16261 gtgagcaatg gtgaacttac cggtggacgt tatgccgtgg cccgccacgt tggcgagctg
    16321 gacgatattt cacacacgat atggggcatt attcgccact ggctgcctgc aagcggcgag
    16381 aaaatgcgta aagcaccgat tctgtttcac tacaccaatc ttgccgaagg ggtgacagag
    16441 cagcgactgg aaacggatgt ttatgtgccg ttggcgtgat ggggaatctt taccggagtg
    16501 tgagccaaat cactcgcgat aagtttgagc gaacaatgtc acaggcgtat tgttgttggg
    16561 cttataattt ctccggtaag gaataccatg tatacgcgaa atttattatc cggtgcgccg
    16621 taagccgtct cgggggtatc catgagcaag ccgcacttat ggcaggaggc gcggggcact
    16681 ggtggagaca cagctaaact gggctgaacc gtatacttac agtcgactac tttgatgctt
    16741 taggcttccc tggactcgca taacctcaac ttctcgaatc gcccggagcg gacccccatg
    16801 ccgagtggtg tggcatgggc gcaaccttgt gctgccctct atgccgatca agtctacagc
    16861 aaagagttgt atatcaaggc acattgatgc ctttatcgcc gtcggagcac gtccggcggc
    16921 gctattcacg ctgatctcct gtgactcgac gcggcagata atgtagtatc tccggcaata
    16981 ttgccccttt gaaggctggc gaataagttg aggaatcaga attaatgagc gatatggcag
    17041 agcgccttgc gctacatgaa tttacggaaa acgcctactt aaactactcc atgtacgtga
    17101 tcatggaccg tgcgttgccg tttattggtg atggtctgaa acctgttcag cgccgcattg
    17161 tgtatgcgat gtctgaactg ggcctgaatg ccagagccaa atttaaaaaa tcggcccgta
    17221 ctgtcggtga cgtactgggt aaataccatc cgcacggcga tatcgcctgt tatgaagcga
    17281 tggtcctgat ggcgcagccg ttctcttacc gttatccgct ggttgatggt caggggaact
    17341 ggggcgcgcc ggacgatccg aaatcgttcg cggcaatgcg ttacaccgaa tcccggttgt
    17401 cgaaatattc cgagctgcta ttgagcgagc tggggcaggg gacggctgac tgggtgccaa
    17461 acttcgacgg cactttgcag gagccgaaaa tgctacctgc ccgtctgcca aacattttgc
    17521 ttaacggcac caccggtatt gccgtcggca tggcgaccga tattccaccg cataacctgc
    17581 gtgaagtagc tcaggcggca atcgcattaa tcgaccagcc gaaaaccacg ctcgatcagc
    17641 tgctggatat cgtgcagggg ccggattatc cgactgaagc ggaaattatc acttcgcgcg
    17701 ccgagattcg taaaatctac gagaacggac gtggttcagt gcgtatgcgc gcggtgtgga
    17761 agaaagaaga tggcgcggtg gttatcagcg cattaccgca tcaggtttca ggtgcgcgcg
    17821 tactggagca aattgctgcg caaatgcgca acaaaaagct gccgatggtt gacgatctgc
    17881 gcgatgaatc tgaccacgag aacccgaccc gtctggtgat tgtgccgcgt tccaaccgcg
    17941 tggatatgga tcaggtgatg aaccacctct tcgctaccac cgatctggaa aagagctatc
    18001 gcattaacct caatatgatc ggtctggatg gtcgtccggc ggtgaaaaac ctgctggaaa
    18061 tcctctccga atggctggtg ttccgtcgcg ataccgtgcg ccgccgactg aactatcgtc
    18121 tggagaaagt cctcaagcgc ctgcatatcc tggaaggttt gctggtggcg tttctcaaca
    18181 tcgacgaagt gattgagatc attcgtaacg aagatgaacc gaaaccggcg ctgatgtcgc
    18241 ggtttggcct tacggaaacc caggcggaag cgatcctcga actgaaactg cgtcatcttg
    18301 ccaaactgga agagatgaag attcgcggtg agcagagtga gctggaaaaa gagcgcgacc
    18361 agttgcaggg cattttggct tccgagcgta aaatgaataa cctgctgaag aaagaactgc
    18421 aggcagacgc gcaagcctac ggtgacgatc gtcgttcgcc gttgcaggaa cgcgaagaag
    18481 cgaaagctat gagcgagcac gacatgctgc cgtctgaacc tgtcaccatt gtgctgtcgc
    18541 agatgggctg ggtacgcagc gctaaaggcc atgatatcga cgcgccgggc ctgaattata
    18601 aagcgggtga tagcttcaaa gcggcggtga aaggtaagag taaccaaccg gtagtgtttg
    18661 ttgattccac cggtcgtagc tatgccatcg acccgattac gctgccgtcg gcgcgtggtc
    18721 agggcgaacc actcaccggc aaattaacgt tgccgcctgg ggcgaccgtt gaccatatgc
    18781 tgatggaaag cgacgatcag aaactgctga tggcttccga tgcgggttac ggtttcgtct
    18841 gcacctttaa cgatctggtg gcgcgtaacc gtgcgggtaa ggctttgatc accttaccgg
    18901 aaaatgccca tgttatgccg ccggtggtga ttgaagatgc ttccgatatg ctgctggcaa
    18961 tcactcaggc aggccgtatg ttgatgttcc cggtaagcga tctgccgcag ctgtcgaagg
    19021 gcaaaggcaa caagattatc aacattccat cggcagaagc cgcacgtggc gaggatggtc
    19081 tggcgcaact gtacgttctg ccgccgcaaa gcacgctgac cattcatgtt gggaaacgca
    19141 aaattaaact gcgtccggaa gagctacaga aagtcactgg cgaacgtgga cgccgcggta
    19201 cgttgatgcg cggtttgcag cgtatcgatc gtgttgagat cgactctcct cgccgtgcca
    19261 gcagcggtga tagcgaagag taatatgccg gggagggcgc tgtttcctcc cgctagcaag
    19321 gaacgccgga tgaaacgtta agtcgtcatc cggcagcgta ttcaaccccc ggaaacgata
    19381 aaaattccct tgaaaccgtt gtttattcat gcgttgcgat taacaatacg cttttccaga
    19441 gagcggcttt taacaatgcc ctaacctgat ttcaggtgac gtacaatgcc agtctctcag
    19501 accttcagag ggtgttatgc tatatatctt tcgtcttatt attaccgtga tttacagcat
    19561 cttagtctgt gtattcggct ccatttactg ccttttcagc ccgcgtaacc cgaaacatgt
    19621 ggccaccttt gggcatatgt ttggccgtct tgcgccactg ttcggcctga aagttgagtg
    19681 ccgcaaacct gcagatgctg aaagctacgg caatgctatc tatatcgcta accaccagaa
    19741 caactatgac atggtgacag catcgaacat tgtgcaaccg ccgacggtga cggtaggtaa
    19801 aaagagcttg ctgtggatcc ccttcttcgg gcagttgtac tggttaaccg gcaacttatt
    19861 gatcgacaga aacaatcgca ctaaagctca cggcaccatt gcggaagtcg tgaatcattt
    19921 caaaaaacgc cgtatttcca tctggatgtt cccggaagga acccgcagcc gtggtcgcgg
    19981 cctgctaccg ttcaagactg gagcatttca cgcggcaatt gcggcgggcg tcccgattat
    20041 tcccgtgtgc gtctctacaa cttcgaataa gattaatctt aatcgactgc acaacggtct
    20101 ggtgattgtc gaaatgctgc cgccaattga cgtcagtcag tatggcaaag atcaggttcg
    20161 tgagctggct gcccattgtc gttcgataat ggaacaaaaa atcgccgagc tcgataaaga
    20221 agtcgcagaa cgcgaagccg ccggaaaagt ttaagtcggc aatctgtatt tttgcgggga
    20281 acactttcct gcacggtatt actttagcca gttttacatg gagcaaatat gtcactcagt
    20341 cggcgtcagt tcattcaggc atcggggatt gcactttgtg caggcgctgt tcccctgaag
    20401 gccagcgcag ccgggcaaca gcaaccgcta cccgttccgc cgctacttga atctcgccgt
    20461 gggcaaccgc tgtttatgac tgtacaacgt gcgcactggt catttacgcc agggacacgc
    20521 gcgtcggtct ggggaatcaa tggtcgttac ctggggccga ctatccgcgt ctggaagggc
    20581 gacgatgtta agcttattta cagcaaccgc ctgacagaaa atgtctcaat gacggtggcc
    20641 gggctacagg taccaggccc gctgatgggc ggtccggcac ggatgatgtc gccaaacgct
    20701 gactgggcac ccgtactgcc tattcgccag aacgcagcta ctctgtggta tcacgccaat
    20761 actcccaacc gcacggctca gcaggtctat aacggccttg ccggaatgtg gctggtggaa
    20821 gatgaagtca gcaagtcgct gcctatcccc aaccattatg gtgtggatga ttttccggtc
    20881 attatccagg ataaacggct ggataacttt ggtacgccag aatacaacga accgggaagc
    20941 ggcggctttg ttggtgatac gctgctggtt aacggtgtac aaagcccgta cgttgaagtc
    21001 tcgcgtggct gggtgcgctt gcgactgctg aacgcgtcga actctcgtcg ctatcaacta
    21061 cagatgagcg atggtcgccc gttacatgtg atttctggcg atcagggatt cctgcctgct
    21121 cctgtatcgg tgaagcaact ttcgctggca ccgggcgagc gccgcgagat tctggtggat
    21181 atgagcaacg gcgatgaagt gtcgatcacc tgtggcgaag cggcgagcat tgttgatcgt
    21241 attcgcggct tctttgagcc atccagtatt ctggtttcta ccttggtgct aacgctgcgc
    21301 ccaaccggcc ttctgccgct ggtcacagac agtcttccga tgcgcttgct gccaactgaa
    21361 atcatggctg gttcgccaat tcgcagtcgc gatatcagtc tgggtgatga cccgggtatt
    21421 aatggacagc tgtgggacgt caaccgtatt gatgtcaccg cgcagcaagg aacgtgggaa
    21481 cgctggacgg tacgcgcgga cgagccgcaa gcgttccata ttgaaggcgt aatgttccag
    21541 atccgtaacg tgaatggcgc gatgccgttc ccggaagaca gaggctggaa agataccgtt
    21601 tgggttgacg gacaagtgga gctgcttgtt tatttcggtc agccttcctg ggcgcacttc
    21661 ccgttctact tcaacagtca gacgctggaa atggcggacc gtggctcgat tgggcaactg
    21721 ttggtcaatc cggtaccgta atcttcatac tcgcccgagg agggcgagta tggaatacct
    21781 ttcgatttct ctttcgttcc ggcttataat ccccggcctt tgattatttt tttatcattt
    21841 ctggaagcaa aatgagctct atctccctga tccaaccgga tcgcgacctg ttctcctggc
    21901 cgcagtactg ggccgcctgt tttggaccgg caccgttttt gccgatgtct cgtgaagaga
    21961 tggatcaact tggctgggat agctgcgaca tcattttggt tactggcgac gcgtatgtcg
    22021 atcacccaag cttcgggatg gcgatttgcg gtcgtatgct ggaagcgcag ggctttcgcg
    22081 tcgggatcat cgcccagccg gactggagca gcaaagacga ctttatgcgt ctgggtaaac
    22141 cgaatctgtt ttttggtgtt actgctggca acatggattc gatgatcaac cgctataccg
    22201 ccgatcgccg tttacgtcat gatgatgcct acacgccaga taatgtcgcg ggtaagcgac
    22261 cggatcgcgc cacgctggtt tatacccagc gttgtaaaga ggcgtggaaa gatgtgccgg
    22321 tgatcctcgg cggtattgag gctagcctgc gccgtaccgc gcattatgat tactggtccg
    22381 ataccgtgcg ccgttccgtt ctggtagatt cgaaagccga catgctgatg tttggtaacg
    22441 gtgaacgtcc gctggtggaa gtagcgcatc gtctggcgat gggcgagccg attagtgaaa
    22501 tccgcgatgt gcgtaatacc gcaattatcg taaaagaggc gctgcctggc tggagcggtg
    22561 tggattccac ccgtcttgat accccaggga aaatcgaccc aatcccgcat ccgtatggcg
    22621 aagatttgcc gtgtgcggat aacaaacctg ttgcgccgaa aaagcaggaa gcgaaagctg
    22681 taatcgtgca gccaccgcgc cagaaaccgt gggaaaaaac ctacgtattg ctgccttctt
    22741 tcgagaaagt gaagggcgat aaagtgctgt acgcccatgc ttcgcgcatt ctgcaccacg
    22801 aaaccaaccc aggctgcgcc cgcgcattga tgcaaaaaca cggtgaccgc tatgtgtgga
    22861 tcaacccgcc tgctatcccg ctttctaccg aagagatgga ctgcgttttt gcgctgccgt
    22921 acaagcgcgt gccacatccg gcctatggca atgcccgtat tccggcttac gaaatgatcc
    22981 gtttttcggt caacattatg cgcggctgct ttggcggctg ttctttctgt tctattaccg
    23041 agcacgaagg gcgcattatt cagagccgtt cggaagattc gatcattaat gagatcgaag
    23101 cgatccgcga caccgttcca ggttttacgg gcgtgatttc cgatctcggt gggccaactg
    23161 ccaacatgta tatgttgcgc tgcaaatcgc cacgcgctga acaaacttgt cgccgtttgt
    23221 cgtgcgttta tccggatatt tgtccgcaca tggacactaa ccacgaaccg acgatcaacc
    23281 tctatcgccg tgcgcgtgat ctgaaaggca ttaaaaaaat ccttatcgcc tcaggcgtac
    23341 gttatgacat cgccgtggaa gatccgcgct atatcaaaga gttggcgacc catcacgtcg
    23401 gcggttatct gaagattgct ccggaacata ccgaagaagg gccgttgtcg aagatgatga
    23461 agccgggcat gggcagctat gaccgcttta aagagctgtt cgatacctac tcgaaacagg
    23521 caggtaaaga gcagtatctg atcccgtatt tcatctctgc gcaccccggt acgcgtgatg
    23581 aagatatggt gaatttggcg ctgtggctga aaaagcatcg tttccgtctc gaccaggtac
    23641 agaacttcta cccatcgccg ctggcgaact caaccaccat gtattacacc gggaaaaaac
    23701 cgctggcgaa gattggttat aagagcgaag acgtcttcgt accgaagggc gacaaacagc
    23761 gtcgtttgca taaagcgttg ttgcgttacc acgatccggc aaactggccg ttaatccgcc
    23821 aggcgctgga agcgatgggc aaaaagcatc tgattggcag cccgcgattg cttagtgcct
    23881 gcgccaacca ttgaagagat gcgtgaagct cgtcgccaga accgcaatac ccgtccggcg
    23941 ttgactaaac atacgccgat ggcgactcag cgtcagacgc ctgctacggc aaaaaaagcg
    24001 tcgtctacgc aatctcgtcc ggtgaatgct ggtgcgaaga aacgccctaa agcggcggtt
    24061 ggacgttaaa aaaaatcccc ggcaactgac atgctgccgg ggattttttt atcattctgc
    24121 gtacatacgc aagcagcgca ggcaatcaaa atagtcctgt gcatcaagac gcgtattggc
    24181 tctgttagct tcggatttgg cagcatagat ttgtgggcgt agtgctttca tatcctgctc
    24241 aagccgggcg attttggcat ttaatgtttg tacattgctc gccagttgat tgaccttttg
    24301 ctcattgacg cagccactga gcaagcaggt tgccagaaca acagctccca cggtgaaaat
    24361 cgttttcata tagtgttccc ttttatttat tgttccggct aattgttatt taattcagct
    24421 cattgcttta atctggcggc ggtttttgcc tcaatcagct taagtaggct gccgctgttc
    24481 gggcggataa aatcagcgat ttcccactta ccttcgcgcc gctgtaaaac cagctcttgc
    24541 tcttcattgt tgccattggt cagcgtaaaa cgaacatgaa tgcgggcgga atcggcggtt
    24601 ttctccagga ctcgaatttc atcaacgcgt gaaaatagcg actcttcgcc aaaacagatc
    24661 ggattgtcgt aaataaattg ccgttctatt tgcatatctt catcgggatt attggccgtc
    24721 ttgaaggtgg ttttatagaa ggcattaacc gcctggcggt aagagtcgga aaccacccac
    24781 gtccacggcc agctataatc agcaatatgt tcaaagagtt cagcaacaaa ggattctggc
    24841 tgttctttct gcaacgaagc taaagcggcc agcgtttttt cattctcgct attaactgct
    24901 tgtaagacgc tgccatgatt gctgacaata tcatcaatga cccaacgacc attttccgcc
    24961 accattttca gtgtctgtgt ggtcttttct ttatcatctt taaagatacg aaagcgcaca
    25021 acggcatcgg catgatcgac gtcagtttgg gttatcgcta cgctttctaa caccagatcg
    25081 ccaaaatcct gacaatcaca aaccggatca taatccagcc agccaatatt gcccggtagt
    25141 gtaagattgt tgttcagggt aagcgcctgt tgaatacgcg ctgaagttat cgcccgctct
    25201 ccggtttcac cgaaataagg cgtactggca tctgatttat agttctgata aatctgccgg
    25261 actgtttgtt ctacggtctg tgaaggaggc tgcgcgtgta cggtaaaact tgccagggct
    25321 gctaaaaaca gaagtattat tttcatctgt ttttcacctc tccgttgcgt ttatcccgga
    25381 tatagatgag ttgttctttt aagagcttcc ggctctgcat gatgatgtcc ttatatttgg
    25441 cattcctgaa caaggaagag taacaacggg cgggacgcga ggggaataaa tgatttctga
    25501 aaagtccggt agcggaacat taccgccacc gggagaattt gcacgttagc cgccgaactg
    25561 gtcaggatcg ggaccgagac gcttgccctg atcgagtttt gcaattttgc cgagttcgtc
    25621 tttgtcgaga cggaaatccc agacatcaaa gttttcggca atacgtgaag gtgtgaccga
    25681 tttcgggatc accaccaggc cgctatccag atgccagcgg ataacaatct gcgccggggt
    25741 tttgccgtat ttatctgcca gatcgcgaat gactttctga tcgaaaacgc cttttcctcc
    25801 ttgcgctaat gggctccagg attcggtctg gattttgtgt gtcgcgttcc aggcgtgtag
    25861 ctggcgttgt tgcatcagcg gatgaagttc gatctggttt atcacaggcg tcacgccagt
    25921 ttcatcaatc aggcgttgca ggtgatggat ctggaagttg cacacgccga tgcttttgat
    25981 taatccctct ttttgcaatt cgatcatgcc tttccatgct tcgacataat ggtcgatagc
    26041 gggaacgggc cagtgcatta agtagaggtc gatataatca agctggagtt ttttcaggct
    26101 gtcgagcagg gcttcgcggg ggcacttgtg gtcgtcgttc cacagcttag tggtgatgaa
    26161 cagttcttct ctgttgactg aggcattttt cagggctttg ccgacacctt cttcgttctt
    26221 gtaggccgcg gcggtatcaa tcgagcgata acccacttct aacgcttttt gaatggcggt
    26281 gattacttcc tcattacttg cttgccagac gcccagtccc agctggggca tgacattgcc
    26341 atcctgtagc ttaataacgg ttggattagc catacgttcc tcctttatat gaactcaccg
    26401 gagcaggctc cggtgaggtg ttgtgaactt aagtctggac gaaatgcccg aaaacgaaag
    26461 tttgaggcgt aaaaagctta gcgggcggct ttgtatatac ggcggctgac atccaacgta
    26521 atgtcatgat tttcgcccag ttgggtcatg ccgtgctctt ccagtttttt cagcaaagcc
    26581 gggatggagc tgccgtccag accgtagtcg gagaggtggg tcggcacgcc taattgctca
    26641 aagaaattgc gggttgcggc aatcgcggcg tcaatacgct catcgtcgga accttcagtg
    26701 atgttccaga cgcgttcagc atattgcagc agcttagcgc gcttggtctc gcgtttttca
    26761 ttccacagtg caggcaggac gatagccagt gtttgcgcgt gatccagacc gtgcatcgca
    26821 gtcagttcgt ggcccagcat atgcgttgcc cagtcctgcg gtacgccagc gccaatcaaa
    26881 ccgttcagcg cctgagtcgc cgcccacatg acgttggcgc gcacatcgta gttttctggc
    26941 tctttcaggg ctttcggacc atcttcgatt agcgtcagca aaatgccttc tgcgaaacgg
    27001 tcctgaattt tggcatcaac cggtttggta acatactgtt ccacggtgtg tacaaaggcg
    27061 tccactacgc cgttagccac ctgacgcggc ggcagggtgt aggtataaac cggatcgagc
    27121 acggcaaata ccggctgaac atgtgcagaa tggaacgcct gcttgtcgcc tgtggtttta
    27181 cgggagatca ccgcgcctgc gttggattct gaaccggttg ctggcagcgt cagcacacag
    27241 cccatcggga tggcgctttt aatctcttta ccgcccgttt gcagaatgtg ccacggatc
//
LOCUS       DAJTXY010000042        27231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTXY010000042 DAJTXY010000000
VERSION     DAJTXY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.042"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..453)
                     /locus_tag="OO566_001919"
     CDS             complement(<1..453)
                     /locus_tag="OO566_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6605221.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLA"
     gene            complement(450..1712)
                     /locus_tag="OO566_001920"
                     /pseudo
     CDS             complement(450..1712)
                     /locus_tag="OO566_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(1709..2617)
                     /locus_tag="OO566_001921"
     CDS             complement(1709..2617)
                     /locus_tag="OO566_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6605222.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2813..3580
                     /locus_tag="OO566_001922"
     CDS             2813..3580
                     /locus_tag="OO566_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6605223.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(3631..4283)
                     /gene="pphB"
                     /locus_tag="OO566_001923"
                     /pseudo
     CDS             complement(3631..4283)
                     /gene="pphB"
                     /locus_tag="OO566_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(4389..6950)
                     /gene="mutS"
                     /locus_tag="OO566_001924"
     CDS             complement(4389..6950)
                     /gene="mutS"
                     /locus_tag="OO566_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6605224.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLLRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            7026..7205
                     /locus_tag="OO566_001925"
     CDS             7026..7205
                     /locus_tag="OO566_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605225.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            7237..7590
                     /locus_tag="OO566_001926"
     CDS             7237..7590
                     /locus_tag="OO566_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6605226.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(7627..9705)
                     /gene="flhA"
                     /locus_tag="OO566_001927"
     CDS             complement(7627..9705)
                     /gene="flhA"
                     /locus_tag="OO566_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6605227.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(9779..10789)
                     /gene="hypE"
                     /locus_tag="OO566_001928"
     CDS             complement(9779..10789)
                     /gene="hypE"
                     /locus_tag="OO566_001928"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6605228.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(10786..11907)
                     /gene="hypD"
                     /locus_tag="OO566_001929"
     CDS             complement(10786..11907)
                     /gene="hypD"
                     /locus_tag="OO566_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6605229.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(11907..12179)
                     /gene="hypC"
                     /locus_tag="OO566_001930"
     CDS             complement(11907..12179)
                     /gene="hypC"
                     /locus_tag="OO566_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6605230.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(12170..13042)
                     /gene="hypB"
                     /locus_tag="OO566_001931"
     CDS             complement(12170..13042)
                     /gene="hypB"
                     /locus_tag="OO566_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6605231.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(13046..13396)
                     /gene="hypA"
                     /locus_tag="OO566_001932"
     CDS             complement(13046..13396)
                     /gene="hypA"
                     /locus_tag="OO566_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6605232.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            13608..14069
                     /gene="hycA"
                     /locus_tag="OO566_001933"
     CDS             13608..14069
                     /gene="hycA"
                     /locus_tag="OO566_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6605233.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            14194..14805
                     /gene="hycB"
                     /locus_tag="OO566_001934"
     CDS             14194..14805
                     /gene="hycB"
                     /locus_tag="OO566_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6605234.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPACVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            14802..16654
                     /gene="hycC"
                     /locus_tag="OO566_001935"
                     /pseudo
     CDS             14802..16654
                     /gene="hycC"
                     /locus_tag="OO566_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            16657..17553
                     /gene="hycD"
                     /locus_tag="OO566_001936"
     CDS             16657..17553
                     /gene="hycD"
                     /locus_tag="OO566_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6605235.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            17571..19256
                     /gene="hycE"
                     /locus_tag="OO566_001937"
     CDS             17571..19256
                     /gene="hycE"
                     /locus_tag="OO566_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6605236.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTAFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            19266..19808
                     /gene="hycF"
                     /locus_tag="OO566_001938"
     CDS             19266..19808
                     /gene="hycF"
                     /locus_tag="OO566_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR6605237.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KENFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            19808..20575
                     /gene="hycG"
                     /locus_tag="OO566_001939"
     CDS             19808..20575
                     /gene="hycG"
                     /locus_tag="OO566_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR6605238.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            20572..20982
                     /gene="hycH"
                     /locus_tag="OO566_001940"
     CDS             20572..20982
                     /gene="hycH"
                     /locus_tag="OO566_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR6605239.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            20975..21445
                     /gene="hycI"
                     /locus_tag="OO566_001941"
     CDS             20975..21445
                     /gene="hycI"
                     /locus_tag="OO566_001941"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR6605240.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            21515..22447
                     /locus_tag="OO566_001942"
     CDS             21515..22447
                     /locus_tag="OO566_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605241.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEGGTRTDFLQSGYEDFGGNYIAHKCLHIVNL
                     DLMSYSFRTHKDRTRRCGCLLTIRKLKLKNAHHF"
     gene            22431..22742
                     /locus_tag="OO566_001943"
     CDS             22431..22742
                     /locus_tag="OO566_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR6605242.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            22739..23158
                     /locus_tag="OO566_001944"
     CDS             22739..23158
                     /locus_tag="OO566_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6605243.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(23273..24697)
                     /gene="ascB"
                     /locus_tag="OO566_001945"
     CDS             complement(23273..24697)
                     /gene="ascB"
                     /locus_tag="OO566_001945"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR6605244.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAIGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24706..26154)
                     /gene="ascF"
                     /locus_tag="OO566_001946"
     CDS             complement(24706..26154)
                     /gene="ascF"
                     /locus_tag="OO566_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR6605245.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQPTVAKEVSLN"
     gene            26414..>27231
                     /gene="ascG"
                     /locus_tag="OO566_001947"
     CDS             26414..>27231
                     /gene="ascG"
                     /locus_tag="OO566_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6605246.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSV"
BASE COUNT         6232 a         7209 c         7351 g         6439 t
ORIGIN      
        1 cgccagatcc tgtgcgatgc gtttatcgcc cggtcggtaa tagggcacca gcgggacatt
       61 tcccatccgc atcaccacgt atggtgtgaa cggacgaata acgttgctgc tgtccagccc
      121 ttgcaggcag gaaagcgccg tcgaccatgt gctgtgcaaa tgcaccaccg ctttacagcg
      181 cggattgttg cgatacagcg ccagatgaaa gagcacctct ttcgagggtt tgtcaccact
      241 taaccattcg ccatccgcgg cgactttgga aagccgctgc ggatcgagat tgcccaggca
      301 tgaacctgtc ggtgtcgcca gtaaattccc gtcaggtaaa agcagcgaca gattgccagc
      361 cgaaccggtt gcatagccgc gctgaaagaa tgaactggca atccgcgtca tctcctctcg
      421 caaagactgc tctacttttg cgaaatcgct catgataaaa actctctttg ggctcgtgaa
      481 aaaaaggctt catcaccgaa gttgccagat ttaagggcga gtgagacagg cttatccagt
      541 gcgtttaccc acggcacgcc gggggaaatg gttgggccaa tatgaaaccc ttttatgccc
      601 aggctctgtg tgactacgcc ggaggtctca ccacctgcga caataaagcg tgtcatgcct
      661 tccgctgcta accgcgccgc tagttgagaa aacagagttt ctactgcctg actggctttt
      721 tgtgcaccgt attgctgttg aattgctgcc aatgcgtcag tgctggcggt ggcaaaaacc
      781 agtggagcaa gtacactttc ctggcccaga acccactctg ccagttcgtg agcataagcg
      841 gccagagttt cggttgagag gcagcgtgcc acatcaactt cacgggctgg tgcaatttga
      901 cggtaatgtg ccacctagcg gttggtcatt tgagagcatg aaccggagag cactacgccg
      961 cgcccagcga gcggacgccc tgcttcgcga gcctggttac cgttttcttg cgcccactgc
     1021 cgggccaggc caatcgccag accagaaccg cccgttacca gtggggcatc gcgcaaggct
     1081 tctccctgaa tttccagatg gtgttcggtc agcgcatcaa gcaccgcgta gcggtagccc
     1141 tcttgctgta agcgagccag ctcttgacga acggcatcca caccttgttc gaaaacatgt
     1201 gccgaaacga cgccgcagcg ccctgtggat tgcgcttcaa ccagacgggg aagatagctg
     1261 ttggtcatgg gatttaccgg gtgatggcgc atcccggatt cggccagcag ttgattcatt
     1321 acgaacaaat acccctgata aaccgtacgt ccgttgaccg gcagggccgg agagaagacc
     1381 gtaaacggcg tgtcgagagc atccattaat gcatcggtaa ccgggccaat attacctttc
     1441 gccgtactgt cgaaagtaga gctgtatttg aaatagatct gtttgcaacc ttgctgttgc
     1501 aaccagctca gagccgccag cgattgctgt gtggcttcaa ccaccggaca ggagcgcgtt
     1561 ttcaggctga tcaccagtgc gtcgattgct tccggcattt tacctgttgg aacaccgtta
     1621 atttgtaccg ttggtagacc gttttccacc agaaaactgg cgatatccgt cgcgccggta
     1681 aaatcatcgg cgataacgcc aatcttgatc atgatttcgc tcccggtaga gtgatgccag
     1741 agaaaatctt gataactgcg ctatcgtctt ctttcccgta acccgcgtta ctggcgctgg
     1801 tgaacatatt caatgctgtt gaggccaatg gcagcgggaa gtgcagggct ttggctgtat
     1861 cggcaaccag accaagatcc ttaacaaaaa tatcgacggc tgaatgcggg gtgtaatcgc
     1921 catccaccac atgacgcatc cggttttcga acatccagga atttccggcg gcattggtca
     1981 cgacgtcata catcacatcc agcgggatcc ccgcacgggc tgcaagtgcc atcgcttcgg
     2041 ctccggcagc aatatgtacg cccgctaaca actggtgaat aatttttacg gtcgaaccta
     2101 gtcccggttc tgcacctatg cgataaactt ttccggcaac ggcttccagc acgggtgcca
     2161 gtcgttcaaa ggcaatatcg ctaccggagg ccatgacagt catttcaccg ttagcggctt
     2221 ttactgcacc accagaaact ggcgcatcca gcatttccag atcgaatcca gccagagcgg
     2281 tagcaatttc ttgcgcatca gcactagcga tagtggaaga aaccattact gccgtaccgg
     2341 gtttcagatg ttgtgcaacg cctgtttcac caaacagcac ctgtttaacc tgggccgcat
     2401 tgaccaccag caccagcaga gcgtccagtt tttcggcaaa cgtcgcggcg ttatcagaaa
     2461 ccccgcaagc acctgcctct ttcaacgtag cgcaggcatt gctgttcagg tctgcgcccc
     2521 aggtagaaaa acctgcgcgg acacatgaca gtgctgctcc cattcccatt gaccctaagc
     2581 caacgatacc gacatgaaac tcagatcccg ttttcatctg ctctccttgt taatttaagt
     2641 gatattttgt ttgacattgt gaatataagc gctgggagat aacgatatgg tgagctgatt
     2701 cacataaatt aacattttgt gttattttat gtgaactaag cgttagttgc gccgcgcacg
     2761 tttcgcaggc aaatagcgta gaatgtcagc aggacaaagg aaggagcaaa agttgatacc
     2821 cgtagagcgt cgccaaatca tccttgagat ggtagctgaa aaaggcattg tcagtattgc
     2881 tgaactaacg gacagaatta atgtgtcaca tatgaccatt cgtcgggatt tacaaaaact
     2941 ggagcagcag ggagccgttg tgctggtgtc cggaggcgtc cagtctccgg gacgcgtggc
     3001 gcatgaacct tctcatcagg taaaaactgc gctggcaatg acgcaaaaag cggctattgg
     3061 caagctggcg gcaagtcttg ttcagccggg aagttgtatc tatctggatg cgggaacgac
     3121 cacgttagcg atagcacagc atctgattca catggagtca ctgactgtgg tcacaaacga
     3181 tttcgttatt gcgaactact tgctcgacaa cagtaattgc acaattattc acactggcgg
     3241 tgcagtgtgt cgggaaaacc gttcctgtgt cggggaagcc gctgcgacca tgctgcgcag
     3301 cctgatgatt gatcaggctt ttatttctgc atcgtcatgg agtgtgcggg ggatttctac
     3361 gccagcggaa gataaagtca cggtgaaacg ggcgattgcc agtgctagcc gccagcgagt
     3421 tttggtctgt gatgcgacga aatatggtca ggtggcgaca tggctggcgt taccattaag
     3481 cgagtttgat cagattatta cagacgacgg tttgccggag agtgccagtc gcgcgctggc
     3541 gaagcaggat ctctctttgc tggtagcgaa aaatgaataa tggcctgcaa taacatctgg
     3601 ttactcatgc ttcacagaag aagcatgaga ctaccttatt ttataaaatg acagccgccc
     3661 acttttcggc gatccggtat caatataaat ctggttagcg aacgtctgaa tgttatcaaa
     3721 catcatatgt ccaaatataa aataatcagc gccgtttatt tgttgtaact cgccattaag
     3781 cgagttctgc acacgataaa caggccagag taattcgctc tccgctattt ctttgtcaaa
     3841 gagatattca tcacccggat aatctgcatg tgcgatgaca tattttatta tgtcgttagt
     3901 gatttcaata atatgtggaa ggtgatggaa tttcagcaac agatctattg cctcttgttg
     3961 caatcattta aatcgaaaaa ccagtcacca ccgctggcaa gccacatatt gccatcgccg
     4021 gtcgcgaacg catccagcgc cattgcttcg tggttgcctt taacggagat aaaccagggc
     4081 tggtttagca ggcgcaggac gtcaagactc tccggcccac gatcaatatt atcgccgaca
     4141 gaaataagta agtcggtttc ggggaaaaaa gagagttgat gtaagcggga ttgtaataac
     4201 tgatattcac catgaatatc accaacgacc catatatggc gatagtgatg ggcattgatt
     4261 ttttgatagc gtgtagatgg catggtttta ccctgtaaaa taagctttcc tattatacag
     4321 ggtattttta tttgattcgt cagttgtcgt taatattccc gatagcaaaa gactatcggg
     4381 aattgttatt acaccagact cttcaagcga taaatccact ccagcgcctg acgcggagtc
     4441 agtgaatccg ggtcgagatt ttccagtgct tcgaccgcag gcgaagtttc ttctggcact
     4501 gacagcaaag acatctgcgt accatccact tgcgtcgcgg cggcgttcgg cgaaatgctt
     4561 tccagctcac gcagtttttg ccgtgcgcgc ttaataacct ctttcggtac gcccgccaga
     4621 gcagcaaccg ccagaccgta gcttttgctc gccgcgccgt cctgcacgct gtgcataaag
     4681 gcgatagtgt cgccgtgctc cagcgcatcg agatgtacgt tggcgacgcc ttccattttc
     4741 tccggtagct gggtcagctc gaaatagtgg gtggcaaaca gcgtcaatgc cttaatctta
     4801 ttcgccagat tttctgcgca cgcccacgcc agcgacagac catcgtaggt ggacgttcca
     4861 cgcccgatct catccattaa caccagactg tattcggtgg cgttatgtaa aatattggcg
     4921 gtttcagtca tctccaccat aaaggttgag cgcccggaag ccaggtcatc tgccgcgcct
     4981 acgcgggtaa agatgcgatc gataggtcca atctcgactt tttgtgccgg tacatagctg
     5041 ccgatgtagg ccatcagcgc aatcagtgcg gtctggcgca tataggtact tttaccgccc
     5101 atgttcgggc cggtaataat caacatacgg cgctgcggcg acagattcag cgggttagcg
     5161 ataaatggct cattcagtac ttgttcaact accggatggc gaccttcggt aatgcgaatg
     5221 cccggtttat caatgaaggt cgggcaggtg tagttcaggg tataggcccg ttccgccagg
     5281 ttaaccagca cgtcgagttc cgccagcgcg ctcgcgctct gttgcaacgc ttccagatgc
     5341 ggcaacagca ggtcgaacag ctcttcataa agctgttttt ccagtgccag tgctttgcct
     5401 tttgaggtga gaactttatc ttcgtactct tttagctctg gaatgatgta gcgctcggcg
     5461 tttttcagcg tctggcgacg catgtagttg atgggtgcca gatggctttg cccacggctg
     5521 atttgaatgt agtagccgtg caccgcatta aagccaactt ttagcgtgtc caggccggta
     5581 cgttcacgct cgcggacttc cagacgctcc agataatcgg tcgcgccgtc agccagcgcg
     5641 cgccactcat ccagctcttc gttatagccc gatgcgataa caccaccgtc gcgtaacagc
     5701 accggcggtg tgtcgatgat tgctcgctcc agcagatcgc gcagctcggc aaactctccc
     5761 atcttctcac gtagcgcctg taccggtgca ctatcgacag tttctaactg cgcacgcagc
     5821 tccggcagtt gctggaaagc gtggcgcata cgggccagat cgcgtgggcg agcagttcgt
     5881 aaagccagac gtgccagaat acgttccagg tcgccgacct gacgcagtac cggctgcaac
     5941 tcggcggtga aatcctgcaa tgcgccaata gtttgctggc gctcaagcaa cacgcgagta
     6001 tcgcgcactg gcatatgcag ccagcgtttc agcatacgac tacccatcgg cgtgacggtg
     6061 cagtcgagca cagaagccag cgtattttcc gcaccgcccg ccaggttctg agtaatttcc
     6121 aggttacgac gtgtcgcggc atccataatg atgctgtcct gctcacgttc catggtgata
     6181 gaacgaatat gcggcagggt cgtgcgttgg gtatctttcg catactgcaa cagacaaccg
     6241 gcagcacaaa gtccgcgcgg cgcgttctcg acgccaaaac cgaccagatc gcgggtgcca
     6301 aattgcagat tcaactgctg gcgcgcggtg tcgatttcaa actcccacag cgggcgacgg
     6361 cgcaggccgc gacggccttc aatcagcgac atctcggcga aatcttctgc atacagcagt
     6421 tccgccggat tagtgcgttg cagctctgcc gccatcgttt cgcggtcggc cggttcgctc
     6481 aggcgaaaac gcccggagct gatatccagc gtcgcgtagc cgaaaccttt gctgtcctgc
     6541 cagatagccg ccagcaggtt gtcctgacgc tcctgtaaca gggcttcatc gctgatggtg
     6601 cctggcgtaa cgatacgcac aactttgcgc tcaaccggac ctttgctggt cgccggatcg
     6661 ccaatttgtt cgcagatggc aacggactct ccctgattca ccagtttggc gagatagttt
     6721 tccaccgcat ggtagggaat ccccgccatc gggatcggct ctcccgccga agcaccgcgt
     6781 ttggtcagtg aaatatccag cagttgcgac gcgcgttttg cgtcgtcata aaacagttca
     6841 taaaaatcac ccatccggta aaacagcagg atctcgggat gctgggcttt cagcttgaga
     6901 tactgctgca tcatgggcgt atgggcgtcg aaattttcta ttgcactcat ggggttatgt
     6961 ccggttccct gatattaaat ggggtgtgat ggtttttatt ttcgttgtaa tcacataagg
     7021 cgcacatgat accggagtta atcatttcag gggaagaagc gaacggcgag ttcttgatgc
     7081 tggttccggg aaagatgctg ttttgcagct tttgcgtgat gcttatgaag caagtacgca
     7141 aaattgtatc gattggcgaa cccttcttct ttagagtcat agtgcccttt aagattaaga
     7201 tgtaatataa atgcatctta atgaggtgga ggcagcatgt ccggcaagcg tatctctcgt
     7261 gaaaaactga cgattaaaaa aatgatcgat ctttatcaag cgaaatgccc gcaggcgtca
     7321 gcggagccgg agcattacga ggcgttgttt gtttacgcgc aaaagcggct ggataaatgt
     7381 gtgttcggcg aagagaaacc agcctgtaag cagtgtccgg tgcactgtta ccagcctgca
     7441 aagcgtgaag agatgaaaca gattatgcgt tgggccgggc cgagaatgct gtggcgtcat
     7501 ccgatcttaa ccgtgcgtca tctcatcgat gacaagcgtc cggtgccaga actgccggaa
     7561 aaatatcgtc cgaaaaaacc tcgtgaatag tatgccggac aggcagatct gtccggcaat
     7621 ttgcagttaa atcaatgccg atttatcaat tcccagccgc ttcatccgtg acagcagggt
     7681 cgtgcgtttc agccccagac gttgcgcagc gcctttaggc ccggcaacca cgctgttggt
     7741 ttctttcagc acgcgcacaa tcaactgata ttcatcttcg ccctcctggg cgacaaccgt
     7801 tgcggcaggc ggcgtttcag gctccggtaa agcaatatct ggcaatgaca gctgcagcac
     7861 gttgccgcgt gttagcaata ctgcgcgctc aatgacgttt tccagttcgc gtacgttacc
     7921 cggccactcc atgttgctca aggtgcgcag cgtctcggca ggaatgctgt cgatattgcg
     7981 ccccagacga cgggcaattt tgaaggtaaa ggctttcgcc agcagcggaa tatcttccgg
     8041 acgctcgcgt agtggcggca ggtgaatcgg gaatacgttc aggcggtaat agagatcgct
     8101 acggaactct cggtcggcga ccattttttt cagatcgcgg ttagtcgcgg caattaaacg
     8161 tacgtccgtc tgaatgattt tgttgctgcc gagacgttca aactcctgtt cctgcaatac
     8221 acgcagcaac ttcggctgta actccagtgg catatcgccc acttcgtcga ggaacagaga
     8281 gcttttatcc gccagttcaa aacgaccaat gcgctgggcg ctggcaccgg taaaagcccc
     8341 acgctcatga ccaaacagat cgctttccag caatccggca ggcatcgccg cgcagttcat
     8401 tttgaccatg cggcgattat tacgcccact gagattatgg atcgcacggg caatcagctc
     8461 tttacccgtg ccagtttcac cgaggatcag cacggtactg tcactttgcg ccaccatttc
     8521 aacttgttta agcacgctgt acatggcttc gctgcggcca ataatctcgc caaattcact
     8581 atcaacattg ttgagttgct cggtcagggc gaggttttca tcaaccagcc gttctttcag
     8641 acgatggatt tcctgatagg cgagggcgtt atcgacagcg attgccacac gttcggcaat
     8701 ctggcgcagc aaattcagat tggtagtggt aaacactttc tcttcgcatt gcgccagttt
     8761 cagcacgccc agcatggtgt cgccagacat cagcggtaac aggcacaagg tttgaatctg
     8821 gttgccccag gtgtcgaaca acatgcgttc atagggggct aaatcgtccc gctcgtagag
     8881 attgatcagc agcatctctt tacttttgaa cacgcgttcg gtgagggttc cggcttcatc
     8941 gacttcgctc tgttcgtggg cgggatgttg tttatcaaga tagtgagtgg agtagatgtt
     9001 gagtttgttt ttacggtggc tgcgtaagac aatgctgata tcgtcaatgt cgaaatagta
     9061 atggatttct ttggcgactt cgctgaccag ttcgtccata tccaggcggg aaagcaccgc
     9121 gttggtaatg gcaaccagga tgcggaagtt atcgcgctcc cggcataaca actcatagtc
     9181 gacattgttg acgacgcggc tctggatttg ttcggtgacg acagaaacga tctgcgtaaa
     9241 tgtttgcaga cgattgaact ctttttcgct ccagggacga tcgtcataac gaataaattc
     9301 acagccacca aaaatatgcc ctcccgccgc cagtggcatc aggcaatagt gaccaaattt
     9361 ggggtatagc ccacctgcgg ccagctgcgg ccaggtttca caaaattctt cgtaactgca
     9421 atgcagcgta tcagggcgcg acaaaatgct gcgtaccgga ccgtgtgcca gaacagtttc
     9481 gtcttcatat ttaatggggg tgtctttttc acgcgacgcg taataagacg cacgttgagt
     9541 ctgcgcttgc cacaacacaa tagccgcgtt gtcggcgagc gcagaacgct ttaccagttg
     9601 cgaaagagcc tcacacagcg aggccagatc gggctgctgc aatagtgtcc gagtgatgtc
     9661 gaacaaccct tgctgtccga gatcactcat cggtgtatat gacattttgt tcttccagga
     9721 aagcaccgca aagcggtgca aaaggtcatt gactaactat aggagattta gaattttatt
     9781 agcatatacg cggaagcggt tcggcgtgtg gtaaatcgag ggttcgtttc acgccataca
     9841 gaccggcaag acgaacacct ttacgttcca ccacttcacc aatcagcgcc gcgtctttcc
     9901 ccagtggatg ggaatgtaac gctgccagca cttgctctgc cgcgttgcgt tcaacggcga
     9961 tcaccagttt accttcgttg gcaaagttca gggcgtccag tcccagcaat tcgcaaacgc
    10021 cgcgcacggc aggtttaacc ggcagcgctg attcagaaat ttcaatacca caaccgcagg
    10081 ctgccgcgaa ctcatgaacc accgcgttta caccaccacg ggtggcatca cgcagcgctt
    10141 tcacgccggg aatgtcacgc agcgtctgaa taagcggcgt cagcaccgcg cagtcgctga
    10201 ccagttcgcc atccagcccc agctgctcac gcaggttaag gatagtcgcc ccgtggtcgc
    10261 cgagtgtacc gctaaccagc aatacatcgc ctgcggttag cgtctgtgcg ccccagtgaa
    10321 tattcgccgg aattgcgccc atgccagcgg tgttgataaa cagtttatct gccgcgccgc
    10381 gctgcaccac tttagtatcg ccagtaacga tggcaatgcc tgccgtgcgg gcggtttctg
    10441 ccatgctggt cactgcggct ttcagtgtct ccatcggcaa tccttcttcg aggataaagc
    10501 cacaggagag atagcgcgga atagcgccac tgaccgcaac gtcattcgcg gtgccgcaaa
    10561 tcgccagctt gccgatatta ccgccaggaa agaacagcgg gtcaatgacg taactgtcgg
    10621 tggagaacgc cagacggtcg ccttccgcta ccagctgcgc cagatcaaga cgtgcctgat
    10681 cttcctgctc tgccagccac gggttggcaa aggcttccat aaacaggctg ttgattaatt
    10741 gctgcatcgc ctggccgccg ctgccgtggg cgagttggat attattcacg cttcactctc
    10801 ctgctgacga tactgatacc acgcggcgca cgctccttcg gaggagacca tcagtgcacc
    10861 aaatgcggtt tgaggattac aggtgttacc aaacagcggg cattgatgcg gcttacattt
    10921 gcccgttaat acctcaccac aacgcgcgcg cgggtcatcg cagacctgct gcggtgccgg
    10981 gcggaaatgt gcttcggcat cgaatcgttg ataatccggc gtcaggtgca cgccagaaga
    11041 ttcaatcacg cctaagccgc gccattcgct gtcgccgttg acacagaaca catcggcaat
    11101 cgcctgttgc gccagcaggt taccggcatc cggtaccacc cgacgatact gattctctac
    11161 cttgctgtgg gccgctattt tctgctccac cagcatgacc acgccttgca gtagatcaag
    11221 gggttcgaaa ccagcgacca ccagcggacg atgaaaatcg ctggcgataa aattataggc
    11281 atcagtgccg ataaccatac taacgtggcc tggcgcgagg aacgcgtcga taccgttatc
    11341 cggctgttcc agcaaactgc gcagcgtcgg gataagcgta atgtgctggc agaagaagta
    11401 aaaattctgc acatcacgcg ctttcgcctg ttgcagagtg atggcggtgg tcggcatggt
    11461 ggtttcaaaa cctaagccga agaacaccac tttgcgggtt ggattctcct gcgccagttt
    11521 caacgcatcc atcggcgagt aaacgatgcg cacatcggca ccgcgtgctt ttgcctgcaa
    11581 cagcgatccc tgtttccctg gtacgcgcat ggcgtcgcca aaggtacaga agatgacttc
    11641 cggatggctg gcaatctcca cgcaggtgtc gattctaccc atcggcagta cgcacaccgg
    11701 gcaacccgga ccgtggataa actcaacgtt ttcaggcagt aactggtcga ggccgaattt
    11761 aaaaatggcg tgggtatgac cgccacacac ttccataatc cgcagagggc gttcggcggt
    11821 gtaagagaga tgtgaagcac gttcgcgcag atgctcaatt aactgcatca cctgttccgg
    11881 cgcgcgatat tcatcaacaa aacgcattat ttttcctcgc catacaacag cgcaccgaca
    11941 tccggctcaa cgtcaaacat gttttgtaag gcgtcgagag tgtcgcgtgc ttcggcttca
    12001 ttaattacgc tcatggcaaa gccaacgtga accagtaccc actggcccac gcgcggctga
    12061 ccgttttcat cgcagctgcc gactaacgtt aaatcgacat cgcgctgaat gccgcagacg
    12121 tcgactttcg cctggttgcc gtcaatggtg cggatctggc cgggaacgcc tatgcacatc
    12181 gctgtgtctc cagccagttc agccactggt ccatcccttc gccgctggtg gcggaaataa
    12241 ggatgatttc aatttctgga ttgacttcgc gggcgcaggc gatgcacttc tcaacgtcaa
    12301 agttgagata cggcaacagg tcaactttgt tgagcagcat cagcgaggcg gcggcaaaca
    12361 tatgcggata tttcagcggt ttgtcttcac cttcggtaac ggaaagcacc gctactttgt
    12421 gtttttcacc gagatcgaag ctggccgggc atacgaggtt gccaacgttt tcgataaaca
    12481 gaataccgtt atcgtccagt ggcagacgcg gtgcggcgtc ggcaatcatc tgtgcgtcaa
    12541 gatggcagcc tttaccggtg ttcacctgaa tcgctggtgt accggtagcg cgaatgcgtg
    12601 cggcatcgtt cacggtttgc tggtcgcctt caataaccgc gcacggaacg ctgtctttca
    12661 ggcgcattaa ggtttccgtc agcagggtgg ttttaccgga accagggctg gaaaccaggt
    12721 tgagcaccag ttgcttgcgg gcagcaaagc gcgcgcggtt gcgttcagcc agacggttgt
    12781 ttttgtccag cacgtcaatt tcgacttcca gcatccgacg ctggctcatg cccggtgtgt
    12841 gagtgcccgc ttcgccatga ccgtaatgca ggtcgccttc ttcagtctgg ctgggggtaa
    12901 attcaggcgc tttaatgccg gtgattttca tctgcgggcg tgccgccggg gcaaatggcg
    12961 cgctacgaaa cgcggaatga gggttatgtt catcaccctc gatatacagg ttgccttcac
    13021 cgcaaccgca tgttgtacac atcgctcact cctggtctat ttctatccgc cgaatctgta
    13081 aaccgtcgtc tgccacaatc tgtagcatgt caccatgaca ctgtggacag cggcggacgc
    13141 gctgggtcag tagcgtcaca tactgttggc atgtttcaca ccagcattcg gcctcttgtt
    13201 cttcgaggtg cagtttacaa ccttccgcca cgctgccgcg gcaaaccaga tcaaaacaaa
    13261 aggcaagaga gctggtttcg acacaagaaa atgcgccaat tttgagccag accccagtta
    13321 cgcgttttgc gccgtgtttt gcggcctgct gttcgatcaa ttccagtgcc cgttggcaga
    13381 gggttatttc gtgcatatcg cctcccatta actattgcca gctacaagca ataattgcgc
    13441 cagtgttgat tatccctgcg gtgaataatg tcgatgatgt cgaaatgaca cgtcgacacg
    13501 gcgacgaaat tcatctttag cttaaaaatc tctttaataa caataaatta aaagttggta
    13561 caaaaaatgc ttaaagctgg catctctgtt aaacgagtaa cctgacaatg actatttggg
    13621 aaataagcga gaaagccgat tacatcgcac agcggcatcg tcgcctacag gaccagtggc
    13681 acatctactg caattcgctg gttcagggga tcacgttatc gaaagcgcgc ctgcatcacg
    13741 ccatgagctg cgcgccggac aaagaactct gtttcgtcct ttttgaacat tttcgtattt
    13801 acgtcaccct ggcggatggc tttaacagcc acaccatcga gtattacgtc gaaacaaaag
    13861 atggcgaaga caaacagcgg attgcgcagg cgcaactgag cattgacggc atgattgatg
    13921 gcaaggtcaa catccgcgat cgcgaacagg ttctggaaca ctatctcgaa aaaatcgctg
    13981 gcgtttacga cagcttatac accgccattg aaaacaatgt gccggtgaat ttaagccaac
    14041 tggtaaaggg acaaagcccg gcagcatgag ctgaggcttt gcccgttttg caggcgttac
    14101 gcctgtttgg ggatgggcgt gtcgatgagt gtcgaaaatg acatttcatc ggcatgtttt
    14161 cgtcaaaaat gacaatcacc tgaggaatgc ctggtgaatc gttttgtaat tgccgactcc
    14221 acgctctgta tcggctgcca cacctgtgag gccgcctgtt cagagacgca tcgccagcac
    14281 ggcctgcaat caatgccgcg cctgagagtg atgctcaatg aaaaagaatc tgcgccgcag
    14341 ctctgtcacc actgtgaaga tgcaccctgc gcggtggtct gcccggttaa cgccatcacc
    14401 cgcgttgatg gggccgtgca gttgaatgaa agtctgtgcg taagctgcaa actgtgcggc
    14461 atcgcctgcc cgtttggcgc aattgaattt tccggcagcc gtccgctgga tattccggca
    14521 aacgccaata ccccgaaagc gccaccggca ccgcctgctc cggcgtgtgt cagcacattg
    14581 cttgactggg tgccaggtat tcgcgcgatc gccgtcaaat gtgacctttg tagctttgat
    14641 gaacaaggtc cggcctgtgt gcggatgtgc ccgactaaag ccctgcatct ggtggataac
    14701 accgatatcg cccgcgtcag caaacgtaag cgtgagctga cttttaacac ggactttggc
    14761 gatctcacct tgtttcagca ggctcaaagt ggagaggcta aatgagcgca atttccctga
    14821 tcaatagcgg cgtggcatgg tttgtcgccg ccgctgttct ggcatttctc ttttcttttc
    14881 aaaaagcgct aagtggctgg atagccggaa ttggcggcgc ggttggtagt ctgtatacgg
    14941 cagccgcggg cttcactgta ctgactggct cggttggcgt gagcggtgcg ctgtcgctgg
    15001 taagctacga tgtgcaaatc tccccgctta acgcgatttg gctgattacc ctcggtcttt
    15061 gcggtctgtt tgtcagcctc tataacattg actggcatcg ccacgcgcag gtgaagtgca
    15121 acggcttgca gatcaatatg ttgatggctg ccgccgtctg cgccgtcatc gccagcaacc
    15181 tcggcatgtt cgtggtaatg gccgaagtca tggccttgtg tgcggtgttc ctcaccagca
    15241 acagcaaaga gggcaaactg tggtttgcgc tggggcgtct tggcactctg ctgctggtga
    15301 ttgcttgctg gctgctgtgg cagcgttatg gcacgctgga tctgcgcctg ctggatatgc
    15361 gtatgcaaca gctgccgctc ggttccgata tctggctgct cggttccgat atctggctgc
    15421 tcggtgtgat tggctttggc ctgctggctg ggattattcc gctgcacggc tgggtgccgc
    15481 aggcacatgc taacgcctct gcgccagctg ccgcgttgtt ttctacggta gtcatgaaaa
    15541 ttggcctgct gggcatgtta accctgtcac tgctgggcgg taatgcaccg ctgtggtggg
    15601 ggatcgcgct gctggtgctc ggcatgatca ccgcgttcgt cggtggtctg tatgcgttga
    15661 tggaacacaa catccagcgc ctgctggctt accacaccct ggaaaatatc ggcattatcc
    15721 tgctggggct ggggctgggg ctggcgtaac gggtatcgcg ctcgaacaac cggcgctgat
    15781 tgctcttggc ctggtcggtg gtctgtacca tctgcttaac catagcctgt tcaaaagcgt
    15841 actgttcctc ggggcgggga gcgtctggtt ccgtaccggt catcgcgata tcgaaaaact
    15901 cggtggtatt ggcaagaaaa tgccggttat ctccatcgcc atgttagtcg ggttgatggc
    15961 aatggctgcg ctgccgccac tgaacgggtt tgccggggaa tgggttatct atcaatcctt
    16021 cttcaaactg agcaatagcg gcgcgtttgt tgcccgtctg ctggggccgc tgctcgctgt
    16081 gggtctggca attaccggtg cgctggcggt gatgtgtatg gcgaaagtct atggcgtcac
    16141 gttcctcggc gcgccgcgta ccaaagaagc cgaaaacgcc acctgtgcgc cgctcctgat
    16201 gagcgtaagc gtagtggcac tggcgatttg ctgcgtaatt ggcggtgttg ctgcgccgtg
    16261 gctgctgccg atgctctctg ctgctgtacc tctgcctctg gagcctgcta acaccaccgt
    16321 ttctcaacca atgatcacat tgctgctgat tgcctgcccg ctgctgccat tcatcattat
    16381 ggcgatttgc aaaggcgacc gcctgccgtc acgttcccgc ggtgcggcct gggtgtgcgg
    16441 ctacgaccat gaaaaatcaa tggtgattac cgctcacggt tttgccatgc cggttaaaca
    16501 ggcgtttgcg ccagtgctga aactgcgcaa atggctgaat ccggtgtcgc tggtgccggg
    16561 ctggcagtgc gaaggtagtg cgttgctgtt ccgccggatg gcgctggttg aactggcggt
    16621 actggtggtg attattgttt cacgaggagc ctgagaatga gtgttttata tccgttaatt
    16681 caggcgctgg tgttatttgc cgttgcgccg ctgctctccg gtattacccg cgtggcgcgc
    16741 gcccgtctgc ataaccgtcg cgggccgggc gtgttgcagg agtatcgcga cattatcaaa
    16801 ctgctgggcc gccagagcgt tggcccggat gcctctggct ggatgttccg cctgacgccg
    16861 tatgtgatgg tgggcgtcat gctgactatc ggttctccgc tgccgcaact gggcgatttg
    16921 atcaccttac tgtatctctt cgccatcgcg cgtttcttct ttgccatttc cggtctggat
    16981 accggtagcc cgtttaccgc tatcggtgcg agccgtgaag cgatgcttgg cgtgctggtc
    17041 gaaccgatgt tgctgcttgg tctgtgggtt gccgcacagg ttgccggttc caccaacatc
    17101 agcaacatta ccgacaccgt ttatcactgg ccgctgagcc aaagcatccc gctggtgctg
    17161 gcgctctgcg cctgtgcgtt cgccactttt atcgaaatgg gcaaactgcc gttcgacctg
    17221 gcggaagccg agcaggagtt gcaggaaggt ccgctctccg aatacagcgg tagcggcttt
    17281 ggcgtcatga aatggggtat cagcctgaaa cagctggtgg tgttgcagat gttcgtcggg
    17341 gtgtttattc cgtggggaca aatggaaacc ttcaccgtcg gtggactgct gctggcgctg
    17401 gtgattgcca tcgtcaaact ggtggtcggc gtcctggtta tcgcgctgtt cgaaaacagt
    17461 atggcccgtc tgcgtcttga tattactccg cgcattacct gggctgggtt tggctttgca
    17521 tttttagcgt tcgtctcctt gctggcggcg tgattaaaga gagtttgagc atgtctgaag
    17581 aaaaattagg tcaacattat ctcgccgcgc tgaatgaggc atttccaggc gtcgtgctgg
    17641 accacgcctg gcagaccaaa gatcagctga ctgtcaccgt aaaggtgaac tatctgccgg
    17701 aagtggtgga gtttctctac tacaagcagg gtggctggct gtcggtgctg tttggtaacg
    17761 acgaacgcaa actgaatggt cattacgccg tttattacgt gctgtcgatg gagaagggca
    17821 ccaagtgttg ggtaacggtt cgcgtcgaag ttgacgccaa caaaccggag tatccgtccg
    17881 tgacgccgcg cgttccggcg gcggtgtggg gcgagcgcga agtgcgcgat atgtacggtt
    17941 tgattccggt tggtctgccg gatgactggc cggatgaact ttatccgctg cgtaaagaca
    18001 gcatggatta tcgtcagcgt ccggcaccga ctaccgatgc tgaaacctac gagttcatca
    18061 acgaactggg cgacaagaaa aacaacgtcg tgccgattgg tccgctgcac gtcacttctg
    18121 acgaaccggg tcacttccgt ctgttcgtcg atggcgaaaa cattatcgac gccgactacc
    18181 gtctgttcta cgtccatcgc ggtatggaaa aactggcgga aacccgcatg ggttataacg
    18241 aagtgacctt cctctctgac cgtgtgtgcg ggatctgcgg ctttgcccac agcaccgcct
    18301 acaccacgtc ggtggaaaac gcgatgggta ttcaggtgcc agaacgcgcg cagatgatcc
    18361 gcgccattct gctggaggtg gaacgcctgc actcgcatct gctcaacctc ggcctcgcct
    18421 gtcactttac cgccttcgac tccggcttta tgcagttctt ccgcgtgcgt gaaacctcca
    18481 tgaaaatggc agagatcctt accggtgcgc gtaaaaccta cggcctgaac ctgatcggcg
    18541 ggattcgtca cgatctgctg aaagatgaca tgatccagac ccgccagctg gcgcaacaaa
    18601 tgcgtcgtga agtgcaggag ctggtggatg tgctgctgag tacgccgaac atggaacaac
    18661 gcaccgtcgg cattggtcgt ctggacccgg aaatcgcccg cgatttcagt aacgttggcc
    18721 cgatggttcg cgccagcggt ctcgcccgcg atactcgcgc cgatcacccg tttgtcggtt
    18781 atggcctgct gccaatggaa gtccacagcg agcagggctg cgacgttatt tcccgtctga
    18841 aagtgcgaat caacgaggtc tataccgcgc tgaatatgat cgactacggt ctggataacc
    18901 tgccgggcgg tccgctgatg gtggaaggct ttacctacat tccgcaccgt ttcgcgctgg
    18961 gctttgccga agcgccgcgc ggcgacgata tccactggag catgaccggc gacaaccaga
    19021 agctgtaccg ctggcgctgc cgtgccgcga cctacgcgaa ctggccgacc ctgcgctaca
    19081 tgctgcgtgg caataccgtt tccgatgcgc cgctgattat cggtagcctg gacccttgct
    19141 actcctgtac cgaccgcatg accgtagtcg atgtgcgtaa gaagaagagc aaagtggtgc
    19201 cgtacaaaga actcgagcgt tacagcattg agcgtaaaaa ctcgccgctg aaataaggaa
    19261 tcgccatgtt tacctttatc aaaaaagtca tcaaaaccgg cacggcgacc tcgtcttatc
    19321 cgctggagcc gattgcggtt gataaaaact tccgtggtaa gccagagcag aacccgcagc
    19381 agtgcatcgg ctgcgcggct tgcgtcaatg cctgcccgtc aaacgcctta acggttgaaa
    19441 ctgacctcgc cacaggagag ctggcgtggg agtttaatct cgggcgctgc atcttctgtg
    19501 gacgctgcga agaagtttgc ccgacggcgg cgatcaaact gtcgcaagag tacgaactgg
    19561 cggtgtggaa gaaagaaaac ttcctgcaac agtcccgctt cgcgctgtgc aactgccgcg
    19621 tctgcaatcg tcctttcgcc gtacagaaag agatcgacta cgccattgcg ctgcttaagc
    19681 acaatggtga cagccgcgcg gaaaaccacc gcgaaagctt tgagacttgc ccggaatgta
    19741 agcgccagaa atgcctggtg ccgtccgacc gtattgaact gactcgccat atgaaagagg
    19801 ccatctgatg agcaatttat taggcccccg tgacgccaac ggcattccgg tcccgatgac
    19861 ggtggatgaa tccatcgcca gcatgaaggc gtcgttactg aaaaaaatca aacgttctgc
    19921 ctatgtttac cgcgtggact gcggcggctg caacggctgc gaaatcgaaa ttttcgccac
    19981 gctttctccg ctgttcgatg cagaacgctt cggcattaaa gtcgttcctt caccgcgtca
    20041 tgcggatatt ttactgttta ccggcgcggt cacccgtgca atgcgatccc ctgcgctgcg
    20101 tgcgtggcag tccgcgccgg acccgaaaat ctgtatctca tacggtgcct gcggtaacag
    20161 cggcgggatc ttacacgatc tctactgcgt gtggggcggt acggataaaa ttgtccctgt
    20221 ggatgtttat atccctggct gcccgccaac gcctgccgcc acgctgtacg gctttgcaat
    20281 ggcgctcggc ctgctggagc agaaaattca cgcccgtggg ccgggtgaac tggatgaaca
    20341 accggcggag atcctgcatg gcgatatggt gcagccgctg cgcgtgaaag tggatcgcga
    20401 agcacgtcgc ctggcgggtt atcgttacgg tcgtcagatt gccgatgatt accttacaca
    20461 gttagggcag ggcgaagaac aggttgcacg ctggctggaa gcggaaaacg atccgcgtct
    20521 gaacgagatt gtcagccatc tgaatcatgt tgttgaagag gcgcgtatcc gatgagtgaa
    20581 aaggtggtgt tcagtcaact gagccgtaaa tttattgatg agaacgatgc cacgcccgcc
    20641 gaggcgcagc aggtggtcta ttacagcctg gcgattggtc accaccttgg ggttatcgat
    20701 tgcctggaag cggcgctcac ctgcccatgg gatgaatatc tggcatggat tgccactctg
    20761 gaggcgggca gcgaagcccg ccacaaaatg gaaggcgtac cgaaatatgg cgagatcgtc
    20821 atcgacatta accatgtgcc gatgctggcc aacgcattcg ataaagcccg ggcagcgcaa
    20881 acttcgcagc agcaggaatg gagtacaatg ctgttaagta tgctgcatga tattcatcag
    20941 gaaaacgcca tctatttgat ggtgaggaga ctgcgtgact gacgttttac tctgtgttgg
    21001 caatagcatg atgggcgatg atggcgctgg cccgctgctg gcggaaaagt gcgccgccgc
    21061 gccgaaaggt aactgggtgg tgattgacgg cggtagtgca ccggaaaacg acatcgtcgc
    21121 catccgtgaa ctgcgcccga cacgactgct gattgtcgac gccacggata tggggctaaa
    21181 ccccggcgaa atccgcatca tcgacccgga tgatatcgcc gagatgttta tgatgactac
    21241 ccataacatg ccgttgaatt accttatcga ccagttaaaa gaagacattg gtgaagtgat
    21301 tttcctcggc attcagccgg atatcgtcgg cttttactac ccgatgaccc agccgattaa
    21361 agatgcggta gaaaccgttt atcaacgact ggaaggctgg gaaggaaatg gtggatttgc
    21421 gcagttagcg gtggaagaag aataattctc catcagggaa ttcagggaat tcagggaatt
    21481 cagggaattc agggaattga acatcagaac agggatgtgc gcttttttat tgagcttagt
    21541 gctgcctgca caggccacca gctttaccga atatctgcca atgagtgaca gcgaatacgc
    21601 gcaaaagcgg gcgctgaaac ctctcctgac catgccttat gatgccgagc aaacctggca
    21661 ttttaggaaa gtgggcgttg ctggcgtgac gcttgagaag atgccaaatg aagaagatta
    21721 ctggcaatta accgctaaag accgcgccgg taaatcctgg actgtgcctg tgggtatgct
    21781 ggcaaacata gcgggcaaag ggcaactcta tcgcgccgat cttgatcgta acggcattca
    21841 ggatctggtt atctggttac caagtaccgg taatggtctg gctccttatg cacatttaat
    21901 tcttatgacg ttcacgcgcg aaggccgtcc ctgtgtcttt gaacccaggg gattctacac
    21961 cgcaagcaaa actggcgtag atgatttgct ggatttgcaa ggcaatggtc acacgcaatt
    22021 gctggatatg cagtttaatt ctggttactg gataaccagt ctgtatcagg taaaagacgc
    22081 caaatggcag cgtgttcacg gctggtttga caagttaagc tatcccgcgc tcacgcgttt
    22141 tacttatacc cccaaccgta aactcgtcct caaacccatc gccgggcgcg acccgcaaac
    22201 ggaagatctg gcgcagacgc agcgttgctt gataaagggg gatgtgcttg aggggggaac
    22261 cagaactgac tttttgcaga gtggctatga ggattttggg ggtaattata tcgcgcacaa
    22321 gtgtttacat attgtgaact tggatttgat gagctatagt ttccgaacac ataaggatag
    22381 aactcgcaga tgtggctgtt tacttactat aaggaagtta aaacttaaaa atgcacatca
    22441 tttctaaagc gccatttgaa gagtctgcgc gtaaatatcc taacgacgct ctggctcttc
    22501 aggcattgta tcgggttatt aaggaaacgg atttttctac gccagaagag atgcgaacgg
    22561 cgtttcccaa cctcgacaat tttaaatatc gcaacaaatg gtacgttctg gatgtaggtg
    22621 ggaacaacct aggggtgatt gcctacataa actttgttaa taagcggttc tttgtgaagc
    22681 acataactaa tcacgccgag tatgacaaac tgacacgtta ctacagagag aacaaagaat
    22741 gactgctaat gctgcacacg ctgttaaagc aacaagagaa ctggtcaatg cagtcccctt
    22801 tctgggaggt agtgactcag aagatgatta tcgtgaagca ctggaactgg tcgaatattt
    22861 gattgaagaa gatgacacca atccacttat cgactttctg gcgagccgca ttgcagaata
    22921 tgaaaacaac aaccaagagt ttgcagagtt cgacaaagcc gttgcggcaa tgcctgttgg
    22981 cgtggcgtta cttcgcacgc tgattgatca acataatctg acctatgcgg atttgaagaa
    23041 cgaaatcggc tctaaatccc tggtcagtca gattttgtct ggacaacgat ccctgacgat
    23101 atcccacatt aaggcacttt cagcccgatt tggcgttaag ccggaatggt ttctttgatt
    23161 tttgactaat gccaaatgct aaattgcccg atgcgctgcg cttatcgggc ctacatgtcg
    23221 cacgaactat gtaggccgga taaggcgttc acgccgcatc cggcactgtt acttactcta
    23281 aatcttcccc attactggca atcacttttt tataccacca gaacgatttc ttacgtgtgc
    23341 gcgtcagcgt gccgttgcct gcgtcgtcac ggtctacgta gacaaaaccg tagcgtttgc
    23401 tcatttcacc cgtagaggcg gaaactaaat caatacagcc ccatgtggtg tagcccatca
    23461 gcggaatgcc atctgcaatc gcttcgccta ttgcgcggat atgttcgcgt aaatagctga
    23521 tgcgatagtc atcgttaatc tcgccattgg cagcaagttc atcttttgcg ccgaggccgt
    23581 tttccaccag aaacagcggc ttctgataac gatcgtacat catattcatg gtgatacgca
    23641 gaccgagtgg atcaattccc cagccccagt cgctcacttg cagatacggg ttacgcagcg
    23701 atttcaccac gttcgccgca ctgctgttgt tggcgttcat ctccgccgag gcgcagcgcg
    23761 aggcgtaata gctgaaagag acaaaatcga cggtgttttt caggatctca tcatcgccca
    23821 gcgctttatc tatggttacc cctttttcgc gaaatacgcg ggcagagtaa gccggatacg
    23881 cgccgcgcgc ctgcacatcg ataaaaaaca ggttttcccg atctttctcc agcgccgccc
    23941 agacatcttc cggtttgcag ctgtaagggt agaagttacc gcccgccagc atacagccca
    24001 cctgattttg cgggttaacc tcatgggcga ttttggtggc tagcgcactg gcaaccagct
    24061 ggtgatgcgc ggcctgatat ttcacctgat cctgattttc accttcttca aacaccagac
    24121 ccgcgccgga gaacgggcta tgcaacataa tgttgatttc attgaaggtt agccagtatt
    24181 tcaccagacc atcaaatgct tcaaagcagg ttctggcgta gcggctgaaa aactccacca
    24241 gcttgcggtt acgccaggag ccatattcgg tgaccagatg catcggcaca tcgaagtggc
    24301 acaacgtgac cagcggttcg ataccgtact ttttacactc ttcaaagaca ggacgataaa
    24361 aagcaatgcc ctgctgattg ggcgtgattt catcgtcctg cggaaagaga cggctccagg
    24421 caattgaggt acggaaaacc ttgaatccca tctctgccat cagggcgata tcttctttat
    24481 aacgatgata aaaatccgtc gcctcatggc tgggataaaa ctcgtcatcg cgcaactgaa
    24541 aacgtttttc cagccccagt ttcaccgcca ttcgatgctc gccgtgtggg atcatatcga
    24601 cggtggtcag cccttttccg ccttcacgga acgcaccttc agactggttg gcggcaagcg
    24661 cgccgcccca taaaaaacct tctggaaata ctgacatttt catcctcaat taagacttac
    24721 ttctttggcg acggtcggtt gatgctttcg cgcctgggca gccgcttcct caacaggaat
    24781 atcctcaaag ccgagcaaca gtgtgaggat aaacgacagc accaccgcca gcgccatgac
    24841 cgcgaacacc cagacgatgc tcattggatt cgccggatcg aagaattgca cgctggtaaa
    24901 tagccccggc gctgccattg agtggctggc aagccccgcc ataccggcaa ccgcgccgca
    24961 aataaaaccg ctgataagac tggcgataag cggacgtttc aggcggatcg ccacgccgta
    25021 taacgccggt tcggaaatcc ccgccataat ggctgatgcc gccgcagcca gcgccgtctg
    25081 gcgcagttcc gggtttttcg ttttccacgg caccgccagt gatgaaccgc ccagcgacag
    25141 gttagcgccg atctctgatg gcatgaccat tccttctttg ccggtttcgg caatggtctg
    25201 aatgatggtt ggcgtaaaga cgcggtgcat cccggtcatt accagcagag gccacagcgc
    25261 gcccataatg gcgactgaaa gccagcccag ataaccatga atggtgtaaa ccagcgccga
    25321 aatggcgcta ccgatccaga taccaatcgg gccaatcagc aggattgcca gcggtgcggc
    25381 aatcaacaca atcagcattg gcttgaggaa gtttttggtc accgccggtg taatgcgatc
    25441 cacccagcgt tcgatatatg acaggcacca ggtcatcacc agcgccggga tcaccgtgta
    25501 ggtgtatttc accgcggtga ccgggatcag ggcaaattcg acatgttcac cctgggccgc
    25561 tttcgccatt aattcaataa aactcggatg taccagcaca cccgcgatgg caatcgccag
    25621 cgacatattg gttttaaatt tgatggcggc agatgccgcg accattagcg gcaggaagaa
    25681 gaaagcaccg tcaccaatca cattcagaat ggttaaggtc ggcgatcctt ttgtcagcac
    25741 gccgctcatc tcgaggatca tcgccagcag tttgaccatc gatccgccga taatcgccgg
    25801 gatcagcggt gacatggtgc cgatcagcgc atcgaggatc cccgcaccaa tgcgacgcag
    25861 cgtgagtttg ggtttaccca cgggctgtgc gggctgcata tctcccggca gcaggctgac
    25921 gacttcctga aaggcctgcg aaaccgtatt gccgataatt acctgacact ggttgtcact
    25981 acgtaccacg ccgagcacgc cggggatggt ttttaacgtc gggctgtcga taagctggtc
    26041 atctttgata acaaagcgca aacgcgtcat acagtgcgtg actgccgaga tgttatcaac
    26101 gccgcccagt gccgctatca ccgagcgtgc cagcgccgca taatttttgg ccatcggatt
    26161 ttatcctgtt atcagtaggg tagatgtagt tgttatcagt cgacgtgacg gaaaaatacc
    26221 gatgctgaaa cggaattaag aaaccggttt cacaaaatca tgaatagttg tcactaaata
    26281 agcaagagag ttaattgttc attttttata tttgtgaaac cggtcacctg aatatttttc
    26341 ccgccgattg cgcaaggaca atcggcaaac ggtgcagtac actgcgttga ttgcgtgtaa
    26401 agggatgaaa atgatgacga cgatgctgga agtggcgaag cgcgccgggg tttcaaaagc
    26461 gaccgtttcc cgcgtgcttt caggtaatgg ctacgtcagc caggagacta aagatcgcgt
    26521 gtttcaggcg gtagaagaga gcagttaccg cccaaatttg ctggcccgca atctgtcggc
    26581 gaagagtact cagacgctgg ggctggtagt gaccaacacg ctttatcacg gcatttattt
    26641 tagtgaatta ctgtttcatg ccgcgcgaat ggcggaagag aaagggcgac agttgctatt
    26701 ggcagatggt aaacacagcg ccgaagaaga gcgccaggcg attcagtatc tgctggattt
    26761 gcgctgcgac gccattatga tttacccgcg ttttttaagc gtggatgaga ttgatgacat
    26821 cattgacgcg cacagtcagc cgataatggt gcttaatcgc cgcctgcgca ataatagcag
    26881 ccatagcgtc tggtgcgatc ataaacagac cagctttaac gccgtggcag agttgataaa
    26941 cgccgggcat caggagattg ctttccttac cggctcgatg gattccccca ccagcattga
    27001 acgtcttgcc gggtataaag acgcgctgtc gcagcatggt attgcgctca atgaaaaact
    27061 tatcgctaac ggtaaatgga cgcccgccag cggggccgaa ggggtagaaa cgttgctcga
    27121 acgtggggct aaatttagcg cgttagttgc cagtaacgac gatatggcga taggtgcgat
    27181 caaagcgttg cacgagcgcg gcgtagcggt gccagagcag gtgtcagtta t
//
LOCUS       DAJTXY010000043        27002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTXY010000043 DAJTXY010000000
VERSION     DAJTXY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.043"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..313
                     /locus_tag="OO566_001948"
     CDS             <1..313
                     /locus_tag="OO566_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR6605247.1"
                     /translation="PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTT
                     ERWGIFALIIVETLAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIIS
                     R"
     gene            complement(375..995)
                     /gene="rhtB"
                     /locus_tag="OO566_001949"
     CDS             complement(375..995)
                     /gene="rhtB"
                     /locus_tag="OO566_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6605248.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1106..2128
                     /gene="pldB"
                     /locus_tag="OO566_001950"
     CDS             1106..2128
                     /gene="pldB"
                     /locus_tag="OO566_001950"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6605249.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2136..2936
                     /gene="yigL"
                     /locus_tag="OO566_001951"
     CDS             2136..2936
                     /gene="yigL"
                     /locus_tag="OO566_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6605250.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3012..3911
                     /gene="bioP"
                     /locus_tag="OO566_001952"
     CDS             3012..3911
                     /gene="bioP"
                     /locus_tag="OO566_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6605251.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3799..4752)
                     /gene="metR"
                     /locus_tag="OO566_001953"
     CDS             complement(3799..4752)
                     /gene="metR"
                     /locus_tag="OO566_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6605252.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            4988..7249
                     /gene="metE"
                     /locus_tag="OO566_001954"
     CDS             4988..7249
                     /gene="metE"
                     /locus_tag="OO566_001954"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6605253.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7288..8103)
                     /locus_tag="OO566_001955"
     CDS             complement(7288..8103)
                     /locus_tag="OO566_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6605254.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8365..9126
                     /gene="udp"
                     /locus_tag="OO566_001956"
     CDS             8365..9126
                     /gene="udp"
                     /locus_tag="OO566_001956"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6605255.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9267..10694
                     /gene="rmuC"
                     /locus_tag="OO566_001957"
     CDS             9267..10694
                     /gene="rmuC"
                     /locus_tag="OO566_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6605256.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFCRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSC"
     gene            10789..11544
                     /gene="ubiE"
                     /locus_tag="OO566_001958"
     CDS             10789..11544
                     /gene="ubiE"
                     /locus_tag="OO566_001958"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6605257.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11558..12163
                     /gene="ubiJ"
                     /locus_tag="OO566_001959"
     CDS             11558..12163
                     /gene="ubiJ"
                     /locus_tag="OO566_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6605258.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGIRKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12160..13800
                     /gene="ubiB"
                     /locus_tag="OO566_001960"
     CDS             12160..13800
                     /gene="ubiB"
                     /locus_tag="OO566_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6605259.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            13879..14148
                     /gene="tatA"
                     /locus_tag="OO566_001961"
     CDS             13879..14148
                     /gene="tatA"
                     /locus_tag="OO566_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6605260.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTISDKQADTNQEQAKTEDAKRHDKEQV"
     gene            14152..14667
                     /gene="tatB"
                     /locus_tag="OO566_001962"
     CDS             14152..14667
                     /gene="tatB"
                     /locus_tag="OO566_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6605261.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14670..15446
                     /gene="tatC"
                     /locus_tag="OO566_001963"
     CDS             14670..15446
                     /gene="tatC"
                     /locus_tag="OO566_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6605262.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15488..16270
                     /gene="tatD"
                     /locus_tag="OO566_001964"
     CDS             15488..16270
                     /gene="tatD"
                     /locus_tag="OO566_001964"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6605263.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16267..16755)
                     /gene="rfaH"
                     /locus_tag="OO566_001965"
     CDS             complement(16267..16755)
                     /gene="rfaH"
                     /locus_tag="OO566_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6605264.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            16922..18415
                     /gene="ubiD"
                     /locus_tag="OO566_001966"
     CDS             16922..18415
                     /gene="ubiD"
                     /locus_tag="OO566_001966"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6605265.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18461..19162
                     /gene="fre"
                     /locus_tag="OO566_001967"
     CDS             18461..19162
                     /gene="fre"
                     /locus_tag="OO566_001967"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6605266.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19255..20418)
                     /gene="fadA"
                     /locus_tag="OO566_001968"
     CDS             complement(19255..20418)
                     /gene="fadA"
                     /locus_tag="OO566_001968"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6605267.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20428..22617)
                     /gene="fadB"
                     /locus_tag="OO566_001969"
     CDS             complement(20428..22617)
                     /gene="fadB"
                     /locus_tag="OO566_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6605268.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22807..24138
                     /gene="pepQ"
                     /locus_tag="OO566_001970"
     CDS             22807..24138
                     /gene="pepQ"
                     /locus_tag="OO566_001970"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6605269.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24138..24752
                     /locus_tag="OO566_001971"
     CDS             24138..24752
                     /locus_tag="OO566_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6605270.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24791..26242
                     /gene="trkH"
                     /locus_tag="OO566_001972"
     CDS             24791..26242
                     /gene="trkH"
                     /locus_tag="OO566_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6605271.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26254..26799
                     /gene="hemG"
                     /locus_tag="OO566_001973"
     CDS             26254..26799
                     /gene="hemG"
                     /locus_tag="OO566_001973"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6605272.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6203 a         6877 c         7430 g         6492 t
ORIGIN      
        1 accacaggtt gagctggcga aaagcgggcg cagtttcctg aaaggcttac tgaccaatct
       61 cgccaatccg aaagcgatta tctattttgg ttcggtattc tcattgtttg tcggcgataa
      121 cgttggcact accgagcgct ggggcatttt cgcgttgatt attgtcgaaa cactggcgtg
      181 gtttaccgtc gttgccagtc tgtttgccct gccgcaaatg cgccgtggtt atcaacgtct
      241 ggcgaagtgg attgatggtt ttgccggggc gttatttgcc ggatttggca ttcatttgat
      301 tatttcgcgg tgatgccaga cgcgtcttca gagtaagtcg gataaggcgt ttacgccgca
      361 tccgacatta tttttcacgc atgcctcgcc gatgctaaca gcgctcccac cagcataaac
      421 aacgagccga aaatcttatt cagcgccttc atctgctttg gtcctttaat ccatagagca
      481 atcctttgag caagggtggc gtaaccgatc atcacaataa tatcgaccac aatagtggtg
      541 acgccgagca cgatatactg catcagttgc ggctgttgcg gcatgatgaa ttgcggaaat
      601 agcgccgcca gaaacacaat acttttggga ttggtgagat tcacaaaaac tgcgcgctgg
      661 aacaaatgtc gacgcgattg agtagaggcc agcgatttaa gatcaattgc gccagcggcg
      721 cgccactgct ggattcccag ccaaatcaag taagccgcgc ctgcccactt taacacttca
      781 aacgcaatca ctgagcggga aaatagcgtc cccaacccca cgccaaccag cacaatatga
      841 atcgccagtc cggtctgaag cccagcaata gacgccaccg cgccgcgata accgtggttg
      901 agcgaggtgg tcatagtgtt gattgcacca gagcctggcg acaggcttaa aatgatcgat
      961 gtcagcaggt aggcaaacca ccattctaag gtcatgatga actcccggtg tgtctatttt
     1021 tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca cgcttaaaaa aacgatttta
     1081 cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa aaagactggg aaacaagaga
     1141 aaacgcgttt gctgctttta ccatgggacc gctgactgat ttctggcgtc agcgtgatga
     1201 agcagagttt actggtgtgg atgacattcc ggtgcgcttt gtccgttttc gcgcacagca
     1261 ccatgaccgg gtggtagtca tctgcccggg gcgtattgag agctacgtaa aatatgcgga
     1321 actggcctac gacctgttcc atttggggtt tgatgtctta atcatcgacc atcgcgggca
     1381 gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg catgttaatc gctttaatga
     1441 ttatgttgat gatctggcgg cattctggca gcaggaggtt cagcccggtc cgtggcgtaa
     1501 acgctatata ctggcacatt cgatgggcgg tgcgatctcc acattatttc tgcaacgcca
     1561 tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg tttgggatcg tgattcgtat
     1621 gccgtcattt atggcacggc agatcctcaa ctgggccgaa gcgcatccac gtttccgtga
     1681 tggctatgca ataggcaccg ggcgctggcg cgcgttgccg tttgctatca acgtactgac
     1741 ccacagcaga cagcgatatc gacgtaactt acgcttctat gctgatgacc caacgattcg
     1801 cgtcggtggg ccgacctacc attgggtacg cgaaagtatt ctggctggcg aacaggtgtt
     1861 agccggtgcg ggtgatgacg ccacgccaac gcttctctta caagctgaag aggaacgcgt
     1921 ggtggataac cgcatgcacg accgtttttg tgaactccgc accgccgcgg gccatcctgt
     1981 cgaaggagga cggccgttgg taataaaagg tgcttaccat gagatccttt ttgaaaagga
     2041 cgcaatgcgc tcagtcgcgc tccacgccat cgttgatttt ttcaacaggc ataactcacc
     2101 cagcggaaac cgctctacag aggtttaaat ttcttatgta ccaggttgtt gcgtctgatt
     2161 tagatggcac gttactttct cccgaccata cgttatcccc ttacgccaaa gaaactctga
     2221 agctgctcac cgcgcgcggc atcaactttg tgtttgcgac cggtcgtcac cacgttgatg
     2281 tggggcaaat tcgcgataat ctggagatta agtcttacat gattacctcc aatggtgcgc
     2341 gcgttcacga tctggatggc aatctgattt ttgctcataa cctggatcgc gacattgcca
     2401 gcgatctgtt tggcgtagtc aacgacaatc cggacatcat tactaacgtt tatcgcgacg
     2461 acgaatggtt tatgaatcgc catcgcccgg aagagatgcg cttttttaaa gaagcggtgt
     2521 tcaaatatgc gctgtatgag ccgggattac tggagccgga aggcgtcagc aaagtgtttt
     2581 tcacctgcga ttcccatgaa caactgctgc cgctggagca ggcgattaac gctcgctggg
     2641 gcgatcgcgt caacgtcagt ttctctacct taacctgtct ggaagtgatg gcgggcggcg
     2701 tgtcgaaagg ccatgcgctg gaagcagtgg cgaagaaact gggctacagc ctgaaggatt
     2761 gtattgcgtt tggtgacggg atgaacgacg ccgaaatgct gtcgatggcg gggaaaggct
     2821 gcattatggg cagtgcgcac cagcgtctga aagatctgca tccggagctg gaagttattg
     2881 gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa actctattta tcgtaatcgt
     2941 tctttatttg gtcagttgtc aacctgatac ttcgctacaa tggatactcg ttaatcaaag
     3001 agttttccat tgtggcgcta cttatcatca ccacgattct gtgggccttc tcctttagct
     3061 tttatggcga gtaccttgcg gggcacgtcg atagctattt tgcggtgctg gtgcgcgttg
     3121 gcttggcggc actcgttttt ctgccgtttc tgcgtacccg tagcaatagc ctgaaaacgg
     3181 tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt gatgtatatg ctgagtttcc
     3241 gcgcttatct ctacctgacg gtctccgaat tgttgctgtt caccgtactg acgccgctct
     3301 acatcacgct gatttatgac atcatgagta agcgccgtct gcgctggggc tatgctttta
     3361 gcgccttgct ggcggtgatt ggtgccggga ttattcgcta tgatcaggtc accgaccatt
     3421 tctggactgg cttgttgctg gtgcaactct ccaatatcac ttttgccatc ggcatggtgg
     3481 gttacaaacg cctgatggaa actcgcccga tgccgcagca taacgccttt gcgtggttct
     3541 atcttggcgc gttcctggtg gcggtaattg catggttctt gctgggaaat gcgcagaaaa
     3601 tgccgcaaac cacgttgcaa tggggcattc tggtgtttct tggcgtggtg gcttccggga
     3661 ttggctactt tatgtggaac tacggcgcga cgcaggtgga cgccggaacg ctgggcatta
     3721 tgaataatat gcacgttccg gcagggctgc tggtaaacct ggctatctgg caccaacagc
     3781 cgcactggcc aacgtttatt acaggcgcgc tggtgatcct ggcctcactg tgggtgcatc
     3841 gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga tcgcaggcgt gattgcgcgc
     3901 tgagcgaata aacgcttccg taactggctg acgctgctcg ccatcgcgca cggcggcgta
     3961 cagtcggctc cacaagcctt cgcccagggt tttggtcacc accagaccct ggcgctcaaa
     4021 actctctact acccaatgcg gtagcgcggc aatacccatc cgcgcggcaa ccatttgaat
     4081 caataataag gtgttatcga cgcttttcag tgacgggctg acgcctgccg gctgaagaaa
     4141 atgccgccag acatccagtc gactacgctg caccggataa attaatagcg tctcgctggc
     4201 gagatcttcc ggtgtaatac gcgtttttgc cgccagtgga tggtcaggtg ctaacaccag
     4261 gcgtacttca taatcgaaca tcggcgaata atgcaggcca ctgcgcggca ggatatcgga
     4321 ggtcatcacc agatccagct cgccctgttg caacgcgggc tgcgggtcaa acgtcacgcc
     4381 cgatttaaaa tccatctcta cctgcggcca gttcttatgg aaattttcta aagcgggtgt
     4441 cagccactga atacagctat ggcactcaat ggcaatgcgc agacgcgtct gctgcggttc
     4501 attgcaggct tgcagtgcct ggctgatttg cggcagcacc tggtttgcca gttgcaacag
     4561 gatttctccc tgcggtgtaa agcgtagcgg ctggctctta cgcacaaata gccggaagcc
     4621 aaggcgttgt tccagatcgc taaactggtg agacagggcg gattgcgtct gatgcaacgt
     4681 tgccgcagcg gctgcgagcg agccgcagtt ccgcaacgct tgtagcgttt tcaggtgttt
     4741 tacttcgatc atgaaagtcc ttcacttcgg catgaataat ttgcgcttga ggaatataca
     4801 gtaaccgcca attatggatg tgtaaacatc tggacggcta aaatccttcg tcttttaaat
     4861 ttatggtgcg ttggctgcgt ttctccaccc cggtcactta cttcagtaag ctcccggcga
     4921 tgaataaact tgccgccttc cctaaattca aaatccatag gatttacata taattagagg
     4981 aagaaaaatg acaatattga atcacaccct cggtttccct cgcgttggcc tgcgtcgcga
     5041 gctgaaaaaa gtgcaagaaa gttattgggc ggggaactcc acgcgtgaag aactgctgac
     5101 agtagggcgt gaactgcgtg ctcgtcactg ggatcaacaa aagcaagcgg gtatcgacct
     5161 gctgccggtg ggcgattttg cctggtacga tcatgtactg accaccagtt tgctgctggg
     5221 taatgttccg ccacgtcatc agaacaaaga cggttcggta gatatcgaca ccctgttccg
     5281 tattggtcgt ggacgtgcgc cgactggcga acctgcggcg gcagcggaaa tgaccaaatg
     5341 gtttaacacc aactatcact acatggtgcc ggagttcgtt aaaggccaac agttcaaact
     5401 gacctggacg cagctgctgg aggaagtgga cgaggcgctg gcgctgagcc acaacgttaa
     5461 gcctgtgctg ctggggccgg ttacttacct gtggctgggg aaagtgaaag gtgaacagtt
     5521 tgaccgcctt agcctgctga acgacattct gccggtttat cagcaagtgc tggcagaact
     5581 ggcgaaacgc ggcatcgagt gggtacagat tgatgaaccc gcgctggtac tggaactacc
     5641 acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg ctccagggac aggtgaaact
     5701 gctgctgacc acctattttg aaggcgtaac gccaaatctc gacacgatta ctgcgctgcc
     5761 tgttcagggg ctgcatgtgg accttgtaca tggtaaagat gacgttgttg aactgcacaa
     5821 gcgcctgcct tctgactggc tgctgtctgc gggtctaatc aatggtcgta acgtctggcg
     5881 cgccgatctt actgagaaat atgcgcaaat taaggacatt gtcggcaaac gtgatttgtg
     5941 ggtggcatct tcctgctcgt tgctgcacag ccccatcgac ctgagcgtgg aaacgcgtct
     6001 tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa aaatgccatg aactggcact
     6061 gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg gcagagtgga gcgccccaat
     6121 tcaggcgcgt cgtcactcta ctcgtgtaca taatccggcg gtagaaaagc gtctggcggc
     6181 gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa gtgcgtgctg aagcccagcg
     6241 tgcgcgtttt aaactgccag cgtggccgac caccacgatt ggttccttcc cgcaaaccac
     6301 ggaaattcgt accctgcgtc tggatttcaa aaagggcaat ctcgacgcca acaactaccg
     6361 cacgggcatt gcggaacata tcaagcaggc cattgttgag caggaacgtt tgggactgga
     6421 tgtgctggta catggcgagg ccgagcgtaa tgacatggtg gaatactttg gcgagcacct
     6481 cgacggattt gtctttacgc aaaacggttg ggtacagagc tacggttccc gctgcgtgaa
     6541 gccaccgatt gtcattggtg acattagccg cccggcaccg attaccgtgg agtgggcgaa
     6601 gtatgcgcaa tcgctgaccg acaaaccggt gaaagggatg ctgacggggc cggtgaccat
     6661 actctgctgg tcgttcccgc gtgaagatgt cagccgtgaa accatcgcca aacagattgc
     6721 gctggcgctg cgtgatgaag tggccgatct ggaagccgct ggaattggca tcatccagat
     6781 tgacgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt agcgactggg atgcgtatct
     6841 ccagtggggc gtagaggcct tccgtatcaa cgccgccgtg gcgaaagatg acacacaaat
     6901 ccacactcac atgtgttatt gcgagttcaa cgacatcatg gattcgattg cggcgctgga
     6961 cgcagacgtc atcaccatcg aaacctcgcg ttccgacatg gagttgctgg agtcgtttga
     7021 agagtttgat tatccaaatg aaatcggtcc tggcgtctat gacattcact cgccaaacgt
     7081 accgagcgtg gaatggattg aagccttgct gaagaaagcg gcaaaacgca ttccggcaga
     7141 gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc ggctggccag aaacccgcgc
     7201 ggcactggcg aacatggtgc aggcggcgca gaatttgcgt cgggggtaaa tccaaaccgg
     7261 gtggtaatac cacccggtct tttctcatta cagcgacttc ttcccaccat actgcttaaa
     7321 ccattccagc atacgctgcc agccatcttt cgcagattcg gcatgatagc tcgggcgata
     7381 atcagcgttg aatgcatgcc cggcatccgg gtacacgata atctctgctt tcgcattagc
     7441 agcccgcagc gcctggcgca tcgtttcaac gctctcctgc ggaatgctgt tatcctgacc
     7501 accatataag ccgagaaccg gtgcgttaag atcggttgcg atatcaacag gttgtttcgg
     7561 tgaattcagc gacttgtcgc ccgtcagttt gccgtaccac gccactgcgg cttttagctg
     7621 tggattatgc gcggcataca gccaggtgat acgtccaccc cagcagaatc cggtgatcat
     7681 taaacgatga acatcgccgc cgttgcggga agcccaactg gcgacatgat cgagatcggc
     7741 cagcacctgc gagtcaggca cttttgctac cagaccgcta agcaacgtgg ggatatcggc
     7801 aaaatcattc ggatcgcctt cgcggaagta aagttcaggt gcgatagcca gatacccctc
     7861 cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc acgccaaaaa tttcctgcac
     7921 tacaatgacc actggcagtg ggccatcgct ttgctttggt ctggcatggt aagcaggcat
     7981 gttatcccct tgtgaaggga tagaggtgaa gcccgccaca atcgcgtcgt ccggggtctg
     8041 aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat ccagattgtt gtgttgttgc
     8101 catggtattc tccgtaccct tataaaaatg ttgcgcaatg ttaactatag tcagcatgca
     8161 acaaatcaca ttgcctgaat cggctcatct tttatgcagt cctgcagaat gaagagtgat
     8221 ttatgtgata tgaatcactt ttggtgggta aatttatgca acgcatttgc gtcatggtga
     8281 tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt ctttttgctt cttctgacta
     8341 aaccgattca cagaggagtt gtatatgtcc aagtctgatg tttttcatct cggcctcact
     8401 aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg gcgacccgga tcgtgtggaa
     8461 aagatcgccg cgctgatgga taagccggtt aagctggcat ctcaccgcga attcactacc
     8521 tggcgtgcag agctggatgg taaacctgtt atcgtctgct ctaccggtat cggcggcccg
     8581 tctacctcta ttgctgttga agagctggca cagctgggca ttcgcacctt cctgcgtatc
     8641 ggtacaacgg gcgctattca gccgcatatt aatgtgggtg atgtcctggt taccacggcg
     8701 tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc tggaattccc ggctgtcgct
     8761 gatttcgaat gtacgactgc gctggttgaa gctgcgaaat ctattggcgc gacaactcac
     8821 gttggcgtga cagtttcttc tgataccttc tacccaggtc aggaacgtta cgatacttac
     8881 tctggtcgcg tagttcgtca ctttaaaggt tctatggaag agtggcaggc gatgggcgta
     8941 atgaactatg aaatggaatc tgcaactctg ctgaccatgt gtgcaagtca gggcctgcgt
     9001 gccggtatgg tagcgggtgt tatcgttaac cgcacccagc aagagatccc gaatgctgag
     9061 acgatgaaac aaactgaaag ccatgcggtg aaaatcgtgg tggaagcggc gcgtcgtctg
     9121 ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg ccttttgtat ttttgcgtag
     9181 cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca gcacaattct attttgtgcg
     9241 ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat catggtttac gcagttattg
     9301 cgttggtggg tgtggcaatt ggctggctgt ttgccagtta tcaacatgcg cagcaaaaag
     9361 ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt aagcgcggca aaacaacaaa
     9421 ttacccaaag cgagcactgg cgtgcagagt gtgaattgct caataacgaa gtgcgcagcc
     9481 tgcaaagtat taacacctct ctggaggccg atctgcgtga agtaaccacg cggatggaag
     9541 ccgcacagca acatgctgac gataaaattc gccagatgat taacagcgag cagcgcctca
     9601 gtgagcagtt tgaaaacctc gccaaccgta tttttgagca cagcaatcgc cgggttgatg
     9661 agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct acgtgaacaa ctggacggtt
     9721 tctgccgtca ggttcaggac agcttcggta aagaagcaca agaacgccat accctgaccc
     9781 acgaaattcg caatctccag caactcaacg cgcaaatggc ccaggaagcg atcaacctga
     9841 cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg gggcgaggta gtattgacgc
     9901 gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata tgaaacccag gtcagcatcg
     9961 aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt gcggctgccg cagggaaaag
    10021 atgtggtgat cgacgccaaa atgacgctgg tcgcctatga acgctatttt aacgccgaag
    10081 acgactacac ccgcgaaagc gcgctacagg aacatatcgc gtcggtgcgt aaccatatcc
    10141 gtttgctggg acgcaaagat tatcaacagc tgccggggct gcgaactctg gattacgtgc
    10201 tgatgtttat tcccgttgaa cccgcttttt tactggcgct tgaccgccag ccggagctga
    10261 tcaccgaagc gttgaaaaac aatatcatgc tggttagccc gactacgctg ctggtggcgt
    10321 tgcgtactat tgccaacctg tggcgttatg agcatcaaag ccgtaacgcc cagcaaatcg
    10381 ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt catcgatgac atgtccgcga
    10441 ttggtcaaag tctcgacaaa gcgcaggata actaccgcca ggcaatgaaa aaactctctt
    10501 caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg cggtttagga gtagaaatta
    10561 aacgcgagat taatccggat ttggctgaac aggcggtgag ccaggatgaa gagtatcgac
    10621 ttcggtcggt tccggggcag ccgaatgatg aagcttatca acgcgatgat gaatataatc
    10681 agcagtcgtg ctagcccatt aggggtagtt aagccgggta gaaatctagg gcatcgacgc
    10741 ccaatctgtt acacttctgg aacaattttt tgatgagcag gcattgagat ggtggataag
    10801 tcacaagaaa cgacgcactt tggttttcag accgtcgcga aggaacaaaa agcggatatg
    10861 gtcgcccacg ttttccattc cgtggcatca aaatacgatg tcatgaatga tttgatgtca
    10921 tttggtattc atcgtttgtg gaagcgattc acgattgatt gcagcggcgt acgccgtggg
    10981 cagaccgtgc tggatctggc tggtggcacc ggcgacctga cagcgaaatt ctcccgcctg
    11041 gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg aatccatgct caaaatggga
    11101 cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg agtatgttca ggcgaacgct
    11161 gaggcgctgc cgttcccgga taacaccttt gattgcatca ccatttcgtt tggtctgcgt
    11221 aacgtcaccg acaaagataa agcactgcgt tcaatgtatc gcgtgctgaa acccggcggc
    11281 cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc cgctgagcaa agcctatgat
    11341 gcatactcct tccatgtgct gccgcgtatt ggctcactgg tcgcgaacga cgccgacagc
    11401 taccgttatc tggcagaatc catccgtatg catcccgatc aggataccct gaaagccatg
    11461 atgcaggatg ccggattcga aagtgtcgac tactacaatc tgacggcagg ggttgtggcg
    11521 ctgcatcgtg gttataagtt ctgacaggag accggaaatg ccttttaaac ctttagtgac
    11581 ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc tcacccgcgc tgaaaacagc
    11641 ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta aaaggctttt cgacgtcatt
    11701 gattctggtg ttcagcgaac gccaggttga tgtactgggc gaatgggcag gcgatgctga
    11761 ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt cgcgatcgcc agcagcttac
    11821 cgcactgatt cgcagtggtg agctggaagt gcagggcgat attcaggtgg tgcaaaactt
    11881 cgttgcgctg gcagatctgg cagagttcga ccctgcggaa ctgctggccc cttataccgg
    11941 tgatatcgcc gctgaaggaa tcagaaaagc catgcgcgga ggcgcaaagt tcctgcatca
    12001 cggcattaag cgccagcaac gttatgtggc ggaagccatt actgaagagt ggcgtatggc
    12061 acccggtccg cttgaagtgg cctggtttgc ggaagagacg gctgccgtcg agcgtgctgt
    12121 tgatgccctg accaaacggc tggaaaaact ggaggctaaa tgacgcctgg tgaagtacgg
    12181 cgcctatatt tcatcattcg cactttttta agctacggac ttgatgaact gatccccaaa
    12241 atgcgtatca ccctgccgct acggctatgg cgatactcat tattctggat gccaaatcgg
    12301 cataaagaca aacttttagg tgagcgacta cgactggccc tgcaagaact ggggccggtt
    12361 tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc tttttccacc gcatattgcc
    12421 gatcagctgg cgttattgca ggacaaagtt gctccgtttg atggcaagct ggcgaagcag
    12481 cagattgaag ctgcaatggg cggcttgccg gtagaagcgt ggtttgacga ttttgaaatc
    12541 aagccgctgg cttctgcttc tatcgcccag gttcataccg cgcgattgaa atcgaatggt
    12601 aaagaggtgg tgattaaagt catccgcccg gatattttgc cggtcattaa agcggatctg
    12661 aaacttatct accgtctggc tcgctgggtg ccgcgtttgc tgccggatgg tcgccgtctg
    12721 cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga ttgatgaact gaatttgctg
    12781 cgggaatctg ccaacgccat tcagcttcgg cgcaattttg aagacagccc gatgctctac
    12841 atcccggaag tttaccctga ctattgtagt gaagggatga tggtgatgga gcgcatttac
    12901 ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg gcactaacat gaaattgctg
    12961 gcggaacgcg gcgtgcaggt gttcttcact caggtctttc gcgacagctt tttccatgcc
    13021 gatatgcacc ctggcaacat cttcgtaagc tatgaacacc cggaaaaccc gaaatatatc
    13081 ggcattgatt gcgggattgt tggctcgcta aacaaagaag ataaacgcta tctggcagaa
    13141 aactttatcg ccttctttaa tcgcgactat cgcaaagtgg cagagctaca cgtcgattct
    13201 ggctgggtgc caccagatac caacgttgaa gagttcgaat ttgccattcg tacggtctgt
    13261 gaacctatct ttgagaaacc gctggccgaa atttcgtttg gacatgtact gttaaatctg
    13321 tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc aactggtgtt actccagaaa
    13381 accctgctct atgtcgaagg ggtaggacgc cagctttatc cgcagctcga tttatggaaa
    13441 acagcgaagc ctttcctgga gtcgtggatt aaagatcagg tcggtattcc tgccctggtg
    13501 agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa tgccagaact gcctgaactg
    13561 gtttacgaca gtttgcgcca gggcaagtat ttacagcaca gtgttgataa gattgcccgc
    13621 gagcttcagt caaatcatgt acgtcaggga caatcgcgtt attttctcgg aattggcgct
    13681 acgttagtat taagtggcac attcttgttg gtcagccgac ctgagtgggg gctgatgccc
    13741 ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg gttggcgcaa aacacgctga
    13801 ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc ggctttgttt aatcatcatc
    13861 taccacagag gaacatgtat gggtggtatc agtatttggc agttattgat tattgccgtc
    13921 atcgttgtac tgctttttgg caccaaaaag ctcggctcca tcggttccga tcttggtgcg
    13981 tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac caaagcagga taaaaccagt
    14041 caggatgctg attttactgc gaaaactatc tccgataagc aggcggatac gaatcaggaa
    14101 caggctaaaa cagaagacgc gaagcgccac gataaagagc aggtgtaatc cgtgtttgat
    14161 atcggtttta gcgaactgct attggtgttc atcatcggcc tcgtcgttct ggggccgcaa
    14221 cgactgcctg tggcggtaaa aacggtagcg ggctggattc gcgcgttgcg ttcactggcg
    14281 acaacggtgc agaacgaact gacccaggag ttaaaactcc aggagtttca ggacagtctg
    14341 aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg aactgaaagc gtcgatggat
    14401 gaactacgcc aggccgcgga gtcgatgaag cgttcctacg ttgcaaacga tcctgaaaag
    14461 gcgagcgatg aagcgcacac catccataac ccggtggtga aagataatga agctgcgcat
    14521 gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt cgccggaaca gaagccagaa
    14581 accacgccag agccggtggt aaaacctgct gcggacgctg aaccgaaaac cgctgcacct
    14641 tccccttcgt cgagtgataa accgtaaaca tgtctgtaga agatactcaa ccgcttatca
    14701 cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat tatcgcggtg atcgtgatat
    14761 tcctgtgtct ggtctatttc gccaatgaca tctatcacct ggtatccgcg ccattgatca
    14821 agcagttgcc gcaaggttca acgatgatcg ccaccgacgt ggcctcgccg ttctttacgc
    14881 cgatcaagct gacctttatg gtgtcgctga ttctgtcagc gccggtgatt ctctatcagg
    14941 tgtgggcatt tatcgcccca gcgctgtata agcatgaacg tcgcctggtg gtgccgctgc
    15001 tggtttccag ctctctgctg ttttatatcg gcatggcatt cgcctacttt gtggtctttc
    15061 cgctggcatt tggcttcctt gccaataccg cgccggaagg ggtgcaggta tccaccgaca
    15121 tcgccagcta tttaagcttc gttatggcgc tgtttatggc gtttggtgtc tcctttgaag
    15181 tgccggtggc aattgtgctg ctgtgctgga tggggattac ctcgccagaa gacttacgca
    15241 aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg gatgttgctg acgccgccgg
    15301 atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg tctgtttgaa atcggtgtct
    15361 tcttctcacg cttttacgtt ggtaaagggc gaaatcggga agaggaaaac gacgctgaag
    15421 cagaaagcga aaaaactgaa gaataaattc aaccgcccgt cagggcggtt gtcatatgga
    15481 gtacaggatg tttgatatcg gcgttaattt gaccagttcg caatttgcga aagaccgtga
    15541 tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg ctactcatca ccggcactaa
    15601 cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag tattcgtcct gttggtcaac
    15661 ggcgggcgtt catcctcacg acagcagcca gtggcaagct gcgactgaag aagcgattat
    15721 tgagctggcc gcgcagccag aagtggtggc gattggtgaa tgtggtctcg actttaaccg
    15781 caacttttcg acgccggaag agcaggaacg cgcttttgtt gctcagctac gcattgccgc
    15841 agaattaaac atgccggtat ttatgcactg tcgcgatgcc cacgagcggt ttatgacatt
    15901 gctggagccg tggctggata aactgcctgg tgcggttctt cattgcttta ccggcacacg
    15961 cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc ggcattaccg gttgggtttg
    16021 cgatgaacga cgcgggctgg agctgcggga attgttgccg ttgattccgg cggagaaatt
    16081 gctgatcgaa acagatgcgc cgtatctgct cccgcgcgat ctcacgccaa agccatcatc
    16141 ccggcgcaac gagccagccc atctgcccca tattttgcaa cgtattgcgc actggcgtgg
    16201 agaagatgcc gcatggctgg ctgccaccac ggatgccaat gtcaaaacac tgtttgggat
    16261 tgcgttttag agtttgcgga actcggtgtt cttcacactg tgcttaatct ctttattaat
    16321 aagattaagc aatagcatgg agcgagcctc accatcgggt tcggtgaaaa tggcctgaaa
    16381 gccttcgaac gcgccttcgg taataatcac cttatcaccc ggataagggg gggccggatc
    16441 gacaatgtct ttcggtttat ataccgatag ctgatgaatc accgccgatg ggactatcgc
    16501 tggcgacgcg ccaaagcgca caaagtggct gacgccacgc gtagcgctga tggtcgtggt
    16561 atgaatcact tctggatcaa attccacaaa caggtagtta gggaataacg gttcactgac
    16621 tgcggtacgt ttaccccgta cgattttttc cagggtgatc atcggagcga ggcaattaac
    16681 cgcctgtctc tcaaggtgtt cctgggcacg ttgaagttgc ccgcgcttgc agtacagtaa
    16741 ataccaggat tgcataatga ctcttatccg tttaatcggg gcgcaaggat agcaaaagct
    16801 ttacgctaag ttaattatat tccccggttt gcgttatacc gtcagagttc acgctaattt
    16861 aacaaattta cagcattgca aagatgaacg ccgtataatg ggcgcagatt aagaggctac
    16921 aatggacgcc atgaaatata acgatttacg cgacttcctg acgctgcttg aacagcaggg
    16981 tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg gaaatcactg aaattgctga
    17041 ccgcactctg cgtgctggtg ggcctgcgct gttgttcgaa aaccctaaag gctactcaat
    17101 gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg gcgatgggca tggggcagga
    17161 agatgtttcg gcgctgcgtg aagttggtaa attattggcg tttctgaaag agccggagcc
    17221 gccaaaaggt ttccgcgacc tgtttgataa actgccgcag tttaagcagg tgttaaacat
    17281 gccgacaaag cgactgcgtg gtgcgccctg ccaacaaaaa atcgtctctg gcgatgacgt
    17341 cgatctcaat cgcattccca ttatgacctg ctggccggaa gatgccgcgc cgctgattac
    17401 ctgggggctg accgtaacgc gcggcccgca taaagagcgg cagaatctgg gcatttatcg
    17461 ccagcagctg attggtaaaa acaaactgat tatgcgctgg ctgtcgcatc gcggcggcgc
    17521 gctggattat caggagtggt gtgcggcgca tccgggcgaa cgtttcccgg tttctgtggc
    17581 gctgggtgcc gatcctgcca cgattctcgg tgcagtcacc cccgttccgg atacgctttc
    17641 agagtatgcg tttgccggat tgctacgcgg caccaaaacc gaagtagtaa agtgtatttc
    17701 caatgacctc gaagtgccag ccagtgccga aatcgtcctg gaagggtata tcgatcctgg
    17761 tgagatggcg ccggaagggc cgtatggtga ccacacaggt tactataatg aagtcgatag
    17821 tttcccggtg tttaccgtga cgcatattac ccagcgtgaa gatgcgattt accattccac
    17881 ctataccggg cgtccgccag atgagcccgc ggtactgggc gtggcgttga acgaagtgtt
    17941 tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat ttttacctgc cgccggaagg
    18001 ctgttcttat cgtctggcgg tagtgacgat caaaaaacag tacgccggac acgcgaagcg
    18061 cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg tacactaaat ttgtgatcgt
    18121 ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg atttgggcga ttaccacccg
    18181 tatggacccg gcgcgggata ctgttctggt agaaaatacg cctattgatt atctggattt
    18241 tgcctcgcct gtctctgggc tgggttcaaa aatggggctg gatgccacga ataaatggcc
    18301 gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa gatccagatg ttgtcacgta
    18361 tattgacgcc atctgggatg aactggctat ttttaacaac ggtaaaagcg cctgatgcgc
    18421 gtttgttttg ccctatttat cgatccgaca gagaaagcgc atgacaacct taagctgtaa
    18481 agtgacctcg gtagaagcta tcacggatac cgtatatcgt gtccgcatcg tgccagacgc
    18541 ggccttttct tttcgtgctg gtcagtattt gatggtagtg atggatgagc gcgacaaacg
    18601 tccgttctca atggcctcga cgccggatga aaaagggttt atcgagctgc atattggcgc
    18661 ttctgaaatc aacctttacg cgaaagcagt catggaccgc atcctcaaag atcatcaaat
    18721 cgtggtcgac attccccacg gagaagcgtg gctgcgcgat gatgaagagc gtccgatgat
    18781 tttgattgcg ggcggcaccg ggttctctta tgcccgctcg attttgctga cagcgttggc
    18841 gcgtaaccca aaccgtgata tcaccattta ctggggcggg cgtgaagagc agcatctgta
    18901 tgatctctgc gagcttgagg cgctttcgtt aaagcaccct ggtctgcaag tggtgccggt
    18961 ggttgaacaa ccggaagcag gctggcgtgg gcgtactggt accgtgttaa cggcggtatt
    19021 gcaggatcat ggtacgctgg cagagcatga tatctatatt gccggacgtt ttgagatggc
    19081 gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg cgggaagatc gcctgtttgg
    19141 cgatgcgttt gcatttatct gagatataaa aaaacccgcc cctgacaggc gggaagaacg
    19201 gcaactaaaa ctttattcag tggcatttag atctatgtcg catccggcaa gtggttaaac
    19261 ccgctcaaac accgtcgcaa taccctgacc cagaccgata cacatcgtcg ccagaccaaa
    19321 ctgaacgtct ttgcgttcca tcagattcag cagcgtggtg ctgatacgcg caccggaaca
    19381 acccagcgga tgacccagcg cgatcgcacc accattgagg ttgatcttct cgtcaatctg
    19441 ctccattagc ccgagatctt taatgcacgg caggatctgc gcggcaaagg cttcgttcat
    19501 ctcaaacacg ccgatatcgc tggcagaaag ccccgctttt ttcagcgcca gcttcgaggc
    19561 cggaaccggg ccgtaaccca taatcgatgg gtcacaacca acgaccgcca tcgaacgcac
    19621 acgagcgcgc ggcttaagac ctaattcatg ggcgcggctt tcactcatca ccagcatggc
    19681 agctgcgcca tcggaaagtg cagaagatgt gcccgccgtt accgtaccgt ttactggatc
    19741 aaacgccgga cgcagcgtgg cgagggcttc cacggtggtt tccgggcgaa tcacttcgtc
    19801 gtaattaaac tgcttcagga cgccgtcggc atcgtgacca ccagttggga tgatttcatt
    19861 tttaaatgcg gccgactgcg tggcggccca ggcgcgggcg tgtgaccgcg cggcaaaggc
    19921 atcctgcatt tcacggctga taccgtgtat acgcgccagc atttctgccg ttaagcccat
    19981 catgcccgcc gctttggcta cattgcggct caggccgggg tgaaaatcga cgccgtgact
    20041 catcggcaca tggcccatat gctccacgcc gccaaccaga catgcctgcg catcgccagt
    20101 catgatcatt cgtgctgcgt catgcagtgc ctgcatggat gaaccacaca agcgattgac
    20161 ggtaaccgcc gggacagagt gcggcacttc tgccagcagc gccgcgttac gggcgatgtt
    20221 aaaaccctgc tccagcgtct gttgcacaca accccagtaa atatcgtcga gggccgccgc
    20281 ttccagcgcc gggttacgcg ccagcaggct acgcattaaa tgagcggaga gatcttccgc
    20341 acgcacgtta cgaaaagcac cgcccttcga acggcccatc ggggtgcgaa ttgcatcgac
    20401 aatgacaacc tgttccattg tgattcctta agccgttttc aggtcgccaa ccggacgggc
    20461 tggctcaacc ggaggatagt acggttcgtt atgacgcgct ttattacgca gaccttccgg
    20521 cacttcatac agcgggccga ggtgctgata ttgctgtgcc atatcgaggt attttgcgct
    20581 accgagggtg tccagccagc ggaacgcgcc gccgtggaac ggagggaagc ccaggccgta
    20641 gaccagcgcc atatccgctt ccgccggagt ggcgataatg ccctcttcca gacagcgcac
    20701 cacttcgttg accatcggga tcatcatgcg ggcgataatc tcttcttcgc tgaaatcgcg
    20761 cttcggttgg ctcacttctg ccagcaggtc ttcaacggcg gcgtcttctt ctttcttcgg
    20821 cttacctttg ctgtcttctt tataacgcca gaaaccgagg ccgttcttct gaccaaagcg
    20881 gttggcatca aacagcgcgt cgatggcatc gcggtaatct ttctgcatcc gctgcgggaa
    20941 gcctgctgcc atgacagcct gagcgtgatg cgcggtatca atgcccacaa cgtccagcag
    21001 atatgccggg cccatcggcc agccaaactg tttttccatc actttgtcga tcttgcggaa
    21061 atccgcgccg tcgcgcagca gctggctgaa accggcgaaa tacgggaaca gcacgcggtt
    21121 aacaaagaag ccggggcagt cgttaaccac aatcggcgtc ttgcccatct tgctcgccca
    21181 ggcgacaact ttcgcgatgg tttcgtcgga gcttttctcg ccgcgaataa tttctaccaa
    21241 cggcattcgg tggaccgggt taaagaagtg catcccgcag aagttttccg ggcgttccag
    21301 cgcgttggcc agttcgctga taggaatggt tgaagtgtta gacgccagca cggtatcctg
    21361 gcgtactttt tgttcggttt ctgccagtac ggcttttttc actttcgggt tttcaacaac
    21421 cgcttctacc acaatatcca cgcggtcaaa tccggcgtag tcgagcgttg ggtggatcgt
    21481 ggagatcacg ccagccagtt tcagaccatc gatcttgccg cgctcaagct gcttgttcag
    21541 cagtttcgcg gcttcggtca tgccgagggt taacgacttg tcgttgatat ctttcatgac
    21601 aaccggcacg cctttccacg cggactggta agcgataccg ccacccatga tgcctgcgcc
    21661 cagcacagca gcctgtttcg gggtttcaac gtctttggtg agtttcttcg ctttgccttt
    21721 tacatattga tcgttaagga aaatgccgac cagtgcgcga gcttcgttgg tatgcgccag
    21781 cgggacaaaa cttttgtttt ccagatttaa ggcttcttca cgaccaaaac gggccgcagc
    21841 ttcaatggtt tttactgcgg tgatgggggc cggataatgt ttccccgccg tttgcgcgac
    21901 cattccttta gcgatggtga agctcatggt ggcttcaatc ttgctcagtt ttagcggttc
    21961 cagcttcggc tgacgtttcg ctttccagtc gaggtcgccg ttaatggcct ggcgtaaaat
    22021 cgccatcgcc ccttcaatca gtttttctgc tttgactacg ccatccacca gaccgatttt
    22081 cagcgcctga tccgcgccga catctttacc ggcggcagtg atttccagtg cactgtcagc
    22141 gcccagcata cgtggcatac gtacagaacc gccaaagccc ggcatgatgc ccagtttggt
    22201 ttccggcaga ccgatgcgca gatccggcgt cgccagacga taatcggtcg ccagcacgca
    22261 ttcgcagcca ccacccagcg catagccgtt gacggcagca atggtcggca ccggcagatc
    22321 ttccaggcga ttaaacacgc tattggcaaa gtgcagccac tgacttaact gttcttcagg
    22381 aacgaggaac agggacaaaa attcggtgat atcagcaccg acgataaagg ctgctttgtt
    22441 cgaacgcagc agcagccctt ttagatctga ttgctgttcc agcacgccga tggcctcgcc
    22501 gaggctggcg acggtcgcag tgtcgagttt attaactgaa cctggggcat caaataccag
    22561 ttcggcaatg ccatcttcca gccagtcaag gtacagggtg tcgcctttgt aaagcatgtc
    22621 agtctcctga atccgcaagg tgatctggtc gtaccagatg catcggagtg tgtgttttgt
    22681 gttaaaaata tgcaaataaa agattaaaga aatgccgatc tgatcacgct cggcagaaat
    22741 cacgctctgg atgaacgatg tgctaagatg cggagactta aggtcaaaaa aacagaaggg
    22801 taaaaaatgg aatcactggc ctcgctctat aaaaatcata tagctacctt acaagaacgg
    22861 actcgcgatg cgctggcgcg cttcaagctg gatgcattac ttattcactc cggcgagctg
    22921 ttcaacgttt ttctcgacga tcatccctat ccgtttaaag tgaacccgca attcaaagcg
    22981 tgggtgccgg taactcaggt gccaaactgc tggttgctgg tggatggcgt gaacaagccg
    23041 aaactgtggt tctatctgcc ggttgattac tggcacaacg tcgaaccgct gccgacctcc
    23101 ttctggactg aagatgtgga agtgatcacg ctgccgaaag ccgatggcat tggtagcctg
    23161 ctgcctgctg cgcgcggcaa tatcggttat atcggtccgg tgccggaacg tgcgctgcaa
    23221 ctgggtattg aggccagcaa catcaacccg aaaggggtga tcgactacct gcattattac
    23281 cgctccttca aaaccgagta cgaactggcc tgtatgcgtg aagcgcagaa aatggcggtc
    23341 aatggtcatc gtgcggcaga agaagcgttc cgttctggca tgagcgagtt cgatatcaat
    23401 attgcctatc tgaccgcgac cggtcatcgt gataccgacg taccttacag caacattgtg
    23461 gcgcttaacg aacacgctgc ggtgttgcat tacaccaaac tggaccacca ggcaccggaa
    23521 gagatgcgca gcttcctgct ggatgccggg gccgaatata acggctatgc ggctgacctg
    23581 acccgtacct ggtcggcaaa aagcgacaac gactacgcac agctggtgaa agacgtaaat
    23641 gatgaacaac tggggctgat cgccaccatg aaagcaggcg tcagctatgt ggattaccac
    23701 atccagttcc atcagcgcat cgccaaactg ctgcgtaaac atcaaatcat caccgatatg
    23761 agtgaagaag cgatggtcga aaacgatctc accggaccgt ttatgccgca cggtattggc
    23821 catccgctgg gcctgcaggt gcatgacgtc gccggtttta tgcaggatga tagcggtaca
    23881 cacctcgcgg caccggcaaa atatccgtac ctgcgctgca cccgtattct ccagccgggc
    23941 atggtgttaa ccatcgaacc gggtatctac ttcattgaat cgcttctggc accgtggcgt
    24001 gaagggcagt tcagcaagca cttcaactgg cagaaaattg aagcactgaa accgttcggc
    24061 ggcattcgta tcgaagacaa cgtggtgatc cacgaaaaca acgtggaaaa catgacccgg
    24121 gatctgaaac tggcgtgatg gaaagctggt taattcctgc ggcaccggtc acggtcgttg
    24181 aagagatcaa aaagagccgc ttcataacga tgttggcgca taccgatggc gttgaggcgg
    24241 cgaaagcgtt tgttgaatcg gtgcgggcag aacaccccga tgcccgccac cattgcgtgg
    24301 cgtgggtggc gggtgcgccg gatgattctc aacagctggg tttctctgac gacggggagc
    24361 cggcgggaac ggcaggtaaa ccgatgctcg cccagctaat gggcagcggc gtcggggaaa
    24421 ttaccgctgt ggtagtgcgc tactacggcg gcatattgct tggcaccggt gggttagtga
    24481 aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct aacgacccaa cgcaagacgc
    24541 cattaaccga atatactttg caatgtgaat atagtcagtt aaccggcatt gaagcgttgc
    24601 tggggcagtg tgacggcaaa attatcaaca gtgattatca ggcattcgtt ctgctgcggg
    24661 tggcgcttcc ggcggcgaaa gtggctgaat tttccgcaaa gctggcggat tttagccgtg
    24721 gttcattgca attgttagcg attgaagaat aatccccact tcgttttgca gaactaagga
    24781 agcggcagag atgcattttc gcgccattac ccgaatcgtt ggactactgg tcatcttatt
    24841 ttcagggacc atgattatcc ctgggctggt agcactcatc taccgggatg gagcgggccg
    24901 cgcttttacc cagacctttt ttgtcgccct cgccattggc tctatgctgt ggtggccgaa
    24961 ccgcaaagag aagggcgaac tgaaatcccg tgaggggttt ctgatagtgg tgctgttttg
    25021 gaccgtgctg ggtagtgtcg gtgcgctccc ttttatcttc tcggaaagcc cgaacctcac
    25081 gattaccgat gcgttttttg aatctttctc tggcctgacc accaccgggg ccactacgct
    25141 ggtggggctg gattcgctcc ctcatgccat cctcttttat cgccagatgc tgcaatggtt
    25201 tggcgggatg gggatcatcg tgttagcggt tgcgatactg cctatcctcg gcgtgggtgg
    25261 gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa gataacaaaa tgcgcccgcg
    25321 aattgcggaa acggcgaaaa ctctgtggtt gatttatgtc ttgctgaccg tcgcctgtgc
    25381 gctggcgttg tggtttgccg gaatggatgc ctttgatgcc atcggccata gctttgcgac
    25441 tatcgctatt ggcggcttct cgacacatga tgccagtatc ggttatttcg acagtccggc
    25501 tattaacact atcattgcta tcttcctgct gatctccggc tgtaactacg gtctgcactt
    25561 ttcactgtta agtgggcgta gtctgaaggt ttattggcgc gatccggaat ttcgcatgtt
    25621 tatcggcgta cagtttacgc tggtggttat ttgtacactc gtactgtggt ttcataatgt
    25681 ctacagttcg gcgctgatga caattaacca ggcgtttttc caggtggtat cgatggcgac
    25741 aaccgccggg tttacgactg acagcattgc ccgctggccg ctctttttgc cggtactgct
    25801 tttatgttca gcttttatcg gcggttgcgc cgggtcaacg ggcggtggcc tgaaagtgat
    25861 ccgcatcctg ctgctgttta agcaggggaa ccgtgagctg aaacgactgg tgcatccgaa
    25921 cgccgtctat agcattaagc tggggaatcg cgcactgccg gaacgtatcc tcgaagccgt
    25981 ttggggattt ttctccgcct atgcattggt gtttattgtc agtatgctgg cgattatcgc
    26041 cacgggcgtg gatgattttt ctgcctttgc ctcagttgtt gcgacattga ataacctggg
    26101 gccagggctt ggcgtggttg ctgataactt taccagtatg aacccggtgg ctaaatggat
    26161 cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt acattgctgg tgctctttac
    26221 cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa cattaattct tttctcaaca
    26281 agggacggac aaacgcgcga gattgcctcc tacctggctt cggaactgaa agaactgggg
    26341 atccagacgg atgtcgccaa tgtgcaccgc attgaagaac cacaatggga aaactatgac
    26401 cgtgtggtca ttggtgcttc tattcgctat ggtcactacc actctgcgtt ccaggaattt
    26461 gtcaaaaaac atgcgacgcg actgaattcg atgccgagcg ccttttattc cgtaaacctg
    26521 gtggcgcgca aaccggagaa gcgtactcca cagaccaaca gctacgcgcg gaagtttctg
    26581 atgaactcgc aatggcgtcc cgatcgctgc gcggtcattg ccggggcgct gcgttaccca
    26641 cgttatcgct ggtacgaccg ttttatgctc aagctgatta tgaagatgtc aggcggtgaa
    26701 acggatacgc gcaaagaagt tgtctatacc gattgggagc aggtggcgaa tttcgcccga
    26761 gaaatcgccc atttaaccga caaaccgacg ctgaaataag cataaagaat aaaaaatgcg
    26821 cggtcagaaa attattttaa atttcctctt gtcaggccgg aataactccc tataatgcgc
    26881 caccactgac acggaacaac ggcaaacacg ccgccgggtc agcggggttc tcctgagaat
    26941 tccgacagag aaagcaaaaa taaatgcttg actctgtagc gggaaagcgt attatgcaca
    27001 cc
//
LOCUS       DAJTXY010000044        25979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTXY010000044 DAJTXY010000000
VERSION     DAJTXY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.044"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            96..1079
                     /locus_tag="OO566_001974"
     CDS             96..1079
                     /locus_tag="OO566_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6605273.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1102..2613
                     /locus_tag="OO566_001975"
     CDS             1102..2613
                     /locus_tag="OO566_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6605274.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2638..3636
                     /locus_tag="OO566_001976"
     CDS             2638..3636
                     /locus_tag="OO566_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6605275.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3669..4763
                     /locus_tag="OO566_001977"
     CDS             3669..4763
                     /locus_tag="OO566_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6605276.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGYRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4775..5647
                     /locus_tag="OO566_001978"
     CDS             4775..5647
                     /locus_tag="OO566_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6605277.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5695..6117)
                     /locus_tag="OO566_001979"
     CDS             complement(5695..6117)
                     /locus_tag="OO566_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6605278.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6214..7416)
                     /locus_tag="OO566_001980"
                     /pseudo
     CDS             complement(6214..7416)
                     /locus_tag="OO566_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7426..8238)
                     /gene="hcaB"
                     /locus_tag="OO566_001981"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(7426..8238)
                     /gene="hcaB"
                     /locus_tag="OO566_001981"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6605279.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8235..8555)
                     /locus_tag="OO566_001982"
     CDS             complement(8235..8555)
                     /locus_tag="OO566_001982"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6605280.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8555..9073)
                     /locus_tag="OO566_001983"
     CDS             complement(8555..9073)
                     /locus_tag="OO566_001983"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6605281.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9070..10431)
                     /locus_tag="OO566_001984"
     CDS             complement(9070..10431)
                     /locus_tag="OO566_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6605282.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10567..11457
                     /gene="hcaR"
                     /locus_tag="OO566_001985"
     CDS             10567..11457
                     /gene="hcaR"
                     /locus_tag="OO566_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6605283.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11617..12756
                     /locus_tag="OO566_001986"
     CDS             11617..12756
                     /locus_tag="OO566_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6605284.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12748..14028)
                     /gene="csiE"
                     /locus_tag="OO566_001987"
     CDS             complement(12748..14028)
                     /gene="csiE"
                     /locus_tag="OO566_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6605285.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRITRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTPQQEQIRKMLESA"
     gene            complement(14219..15073)
                     /locus_tag="OO566_001988"
     CDS             complement(14219..15073)
                     /locus_tag="OO566_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6605286.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMLPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15218..16021)
                     /gene="suhB"
                     /locus_tag="OO566_001989"
     CDS             complement(15218..16021)
                     /gene="suhB"
                     /locus_tag="OO566_001989"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6605287.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGDL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16140..16880
                     /gene="trmJ"
                     /locus_tag="OO566_001990"
     CDS             16140..16880
                     /gene="trmJ"
                     /locus_tag="OO566_001990"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6605288.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17150..17638
                     /gene="iscR"
                     /locus_tag="OO566_001991"
     CDS             17150..17638
                     /gene="iscR"
                     /locus_tag="OO566_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6605289.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17750..18964
                     /gene="iscS"
                     /locus_tag="OO566_001992"
     CDS             17750..18964
                     /gene="iscS"
                     /locus_tag="OO566_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6605290.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            18992..19378
                     /gene="iscU"
                     /locus_tag="OO566_001993"
     CDS             18992..19378
                     /gene="iscU"
                     /locus_tag="OO566_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6605291.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19395..19718
                     /gene="iscA"
                     /locus_tag="OO566_001994"
     CDS             19395..19718
                     /gene="iscA"
                     /locus_tag="OO566_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6605292.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19814..20329
                     /gene="hscB"
                     /locus_tag="OO566_001995"
     CDS             19814..20329
                     /gene="hscB"
                     /locus_tag="OO566_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6605293.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20346..22196
                     /gene="hscA"
                     /locus_tag="OO566_001996"
     CDS             20346..22196
                     /gene="hscA"
                     /locus_tag="OO566_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6605294.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22198..22533
                     /gene="fdx"
                     /locus_tag="OO566_001997"
     CDS             22198..22533
                     /gene="fdx"
                     /locus_tag="OO566_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6605295.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22545..22745
                     /gene="iscX"
                     /locus_tag="OO566_001998"
     CDS             22545..22745
                     /gene="iscX"
                     /locus_tag="OO566_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6605296.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22804..24087
                     /gene="pepB"
                     /locus_tag="OO566_001999"
     CDS             22804..24087
                     /gene="pepB"
                     /locus_tag="OO566_001999"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6605297.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKRVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24229..25005
                     /gene="sseB"
                     /locus_tag="OO566_002000"
     CDS             24229..25005
                     /gene="sseB"
                     /locus_tag="OO566_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6605298.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDCALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25500..>25979)
                     /gene="sseA"
                     /locus_tag="OO566_002001"
     CDS             complement(25500..>25979)
                     /gene="sseA"
                     /locus_tag="OO566_002001"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6605299.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT         6142 a         6498 c         7216 g         6123 t
ORIGIN      
        1 ccctattaac tgtaatattt gagcggcacg cgctgccgct catcacaaaa taattagaat
       61 aatccgttgt taataaaaac cctacaggag gcactatgcc taaaaaaatg agaacaaccc
      121 gtaatctatt attaatggct acgctattag gtagcgcact atttgccagg gctgcggata
      181 aagaaatgac cattggtgca atataccttg atacccaggg atattacgct ggagtgcgtc
      241 agggcgttca ggatgcggca aaagattctt cagtacaggt acagttaatt gaaactaacg
      301 cccaggggga tatttcgaaa gaaagtacct ttgttgatac cctcgtggcg cgtaatgtcg
      361 atgccattat tttatcggca gtgtctgaaa atggcagtag ccgtaccgtt cgtcgcgcca
      421 gtgaagcggg tattccggtg atttgctaca acacctgtat taatcaaaag ggtgtcgata
      481 aatatgtctc ggcgtatctg gtcggcgatc cactggaatt tggtaaaaaa ctgggtaacg
      541 ctgccgccga ttattttatt gccaataaaa ttgaccagcc gaaaattgcc gtcatcaatt
      601 gcgaagcctt tgaagtttgt gtgcagcgac gtaaaggatt tgaagaagta ttaaaatccc
      661 gcgttcccgg cgcgcaaatt gtcgctaacc aagaagggac tgttttagat aaagcgattt
      721 ccgttggtga aaaactgatt atctccacgc cggatctcaa cgccattatg ggggagtcgg
      781 gcggtgcgac actcggcgcg gtaaaagcgg tacgtaatca aaatcaggcc ggaaaaattg
      841 ctgttttcgg ttcggatatg acaaccgaaa ttgctcagga gctggaaaac aatcaggtgc
      901 tgaaagcggt agtggatatt tccggtaaga aaatgggcaa tgctgttttc gcgcaaacat
      961 tgaaggttat caataaacaa gccgacggtg aaaaagtgat tcaggtgcca atcgatctct
     1021 ataccaaaac ggaagatggt aaacagtggc tggcaacgca cgttgatggt ctgccctaat
     1081 cgctgattcg cagaggtccc tatgttcacg gcaacagagg cagtcccggt agcaaaagtg
     1141 gtggcaggaa ataagcgtta ccccggcgtc gttgcgctgg ataacgttaa cttcacgctc
     1201 aataaaggcg aagttcgtgc gctgttaggt aaaaacggcg cgggcaaatc gactctcatt
     1261 cgaatgctta ccggcagcga acgcccggat agcggtgata tctggattgg cgagacgcga
     1321 ctggaaggtg acgaagctac gttgactcgc cgtgccgctg aactgggggt tcgtgcggtt
     1381 tatcaggaat taagtctggt ggaagggctg acggtggcag aaaatctctg ccttggtcaa
     1441 tggcctcgcc gcaacggcat gattgattac ctgcaaatgg cgcaggatgc ccaacgttgc
     1501 ttacaggcgc tgggcgttga cgttagtcct gaacaacttg tttcaacgct aagcccggcg
     1561 caaaagcaac tggtggaaat tgcacgggtg atgaagggcg agccgcgcgt ggtcattctt
     1621 gatgaaccca ccagctcgct tgccagtgca gaagttgaac tggtgatcag tgcggtgaaa
     1681 aagatgtcgg cactgggcgt ggcggtgatt tatgtcagcc accggatgga agaaatccgc
     1741 cgcattgcct cctgtgccac cgttatgcgc gatggtcagg tggcgggcga tgtgatgctc
     1801 gaaaacacct ccacgcatca tattgtgtcg ctaatgctag ggcgtgatca cgttgatatt
     1861 gcgccggttg cccctcagga aattgtggat caggccgtac tggaagtccg tgcgttacgc
     1921 cataagccca agctggagga tatcagcttt acgctacgtc gtggcgaagt gcttggcatt
     1981 gctggcctgc tgggggcagg gcgcagtgaa ttgctgaagg cgattgttgg gctggagacg
     2041 tatgaacagg gcgaaattgt tatcaacggc gagaaaatca tgcgccccga ttacggcgac
     2101 atgctgaaac gcggcattgg ctatacgcca gaaaaccgca aagaagcggg gatcattccc
     2161 tggctgggcg ttgacgaaaa tacagtgctg accaatcggc aaaaaatcag caccaacggt
     2221 gtgctgcaat ggtccaccat ccgccgcctg accgaagagg tgatgcagcg gatgacggtc
     2281 aaggctgcca gtagcgaaac acccatcggc acgctttctg gtggcaatca gcaaaaagtg
     2341 gtgatcggtc gttgggtcta tgccgccagc cagattttgt tgctcgacga gccaacgcgc
     2401 ggcgtcgata tcgaagccaa acagcagatt taccgtattg tccgtgagct ggctgccgaa
     2461 ggaaaaagcg tggtgtttat ctccagtgaa gtggaggaat tgccgctggt gtgtgaccgc
     2521 atcctgttat tacagcacgg cacgttctcg caggagtttc actcgccggt caatgtggat
     2581 gagctgatgt ccgccattct gtctgtgcac tgatgaattt taccgaggaa cctgaagatg
     2641 tctgcttcgt cattaccatt gccgcagggc aagagcgtct cgctcaaaca atttgtcagt
     2701 cgccatatta atgagatcgg tttgctggtg gtgattgcca ttctttatct ggtcttctcc
     2761 ctgaacgcgc ctggctttat ctcattgaat aaccagatga acgtgctgcg cgatgccgcc
     2821 accattggga ttgccgcctg ggcgatgacg ctgattatta tctccggtga aattgatgtc
     2881 agcgttgggc cgatggtggc ttttgtctcg gtgtgcctgg catttttgct gcaatttgaa
     2941 gttccgttgg cgattgcttg tctgctggtg ttgctgttag gcgcgctgat gggaacgctc
     3001 gccggggtgc tgcgcggcgt gtttaacgta ccaagtttcg ttgccacact ggggctgtgg
     3061 agcgctctgc gcggaatggg gctgtttatg accaacgcac agccagtgcc gattgacgaa
     3121 aacgaggtgc tggactggct gggcggacaa tttctcggtg tgccagtatc cgcgctgatc
     3181 atgatggtgt tgtttgcgct gtttgtgttc attagccgaa aaaccgcctt cgggcgctcg
     3241 gtttttgccg ttggcggtaa tgccacggcg gcgcagttgt gcggtattaa cgttcgtcga
     3301 gtgcgcattc ttatctttac cctttcggga ttattagcgg cggtgaccgg cattttgttg
     3361 gcggcacgcc tcggttccgg taacgcaggt gccgcaaacg gtctggagtt tgacgtcatc
     3421 gccgcggtcg tggtcggcgg tacggcactt tccggcggtc gcggttccct gttcggtaca
     3481 ttgcttggtg tgctggtgat tacgctaatc ggtaacggtc tggtgctgct cggtattaac
     3541 tcctttttcc agcaggtggt acgcggcgtc atcatcgtgg tggcggtgct ggcgaatatc
     3601 ttgctgaccc agcgaagcag taaagcgaaa cgctaaacct taacgcccct ggcgcgggtg
     3661 cggcagggat gaaaattaat cgtattgtta atgagggatt tatgaaaacg atgctggcag
     3721 cttatttacc aggaaattca accgtcgatc tgcgggaagt tgcggtgccg acgccgggga
     3781 ttaaccaggt actgatcaaa atgaaatcct ccgggatttg cggaagcgat gtccactata
     3841 tctatcatca acaccgtgcc acggcggcgg cacccgataa accgttatac cagggattta
     3901 tcaacggtca tgaaccgtgc gggcagattg tggcgatggg gcaaggctac cgccatttta
     3961 aagagggcga ccgcgtgctg gtgtatcaca tttctggctg tggtttttgc ccgaactgcc
     4021 gccgcggctt tcctatctct tgtactggca aaggaaaagc ggcttacggc tggcagcgtg
     4081 acggcggtca tgccgaatat ctgctggcgg aagaaaaaga tctgatcctc ctgccggatg
     4141 cgctgagcta cgaagatggt gcgtttatca gttgcggcgt tggcacggcc tatgaaggga
     4201 ttttgcgcgg cgaagtttcc ggcagcgata acgtgctggt ggtcggtctg ggggcggtcg
     4261 gcatgatggc gatgatgctg gcgaaaggtc gcggtgcaaa acggatcatc ggcgttgata
     4321 tgctgccgga acgtctggcg atggcaaaac agttaggggg gatggatcac ggctatttag
     4381 caaccaccga aggtctgccg cagataattg ctgaacttac ccacggtggc gcggatgttg
     4441 cgctcgattg ttccggtaat gccgcaggtc gcttgctggc actgcaatcc actgctgact
     4501 gggggcgggt ggtttacatt ggtgaaaccg gaaaagtgga attcgaggtc agcgctgatc
     4561 tgatgcacca tcaacggcgg attatcggct cctgggtgac cagtctgttc catatggaaa
     4621 aatgcgccca cgatttgact gactggaagc tgtggccgcg taacgccatt acccatcgct
     4681 tctcgctaga acaggcaggt gatgcctatg tgctgatggc gagcggcaaa tgcgggaaag
     4741 ttgtgattaa cttcccggat taaggagtta gcggatgacc atctatacct tatcccacgg
     4801 gtcgctaaag ctggacgttt ctgaccaggg cggcgtaatc gaaggcttct ggcgcgatac
     4861 gacgccgctc ttacgtcctg gtaaaaaaag cggtgtggca accgatgcct catgttttcc
     4921 gctggtgcca tttgcaaacc gggtgagcgg caaccgtttt gtctggcagg ggcgtgagta
     4981 tcaactgcaa ccgaatgttg agtgggatgc gcactatcta cacggcgatg gctggctggg
     5041 cgagtggcaa tgtgtctcgc acagcgatga tagtttgtgt ctggtgtatg aacatcgcag
     5101 cggtgtctat cactatcggg taagtcaggc gtttcattta acggcggata cgctgacggt
     5161 gacgctctct gtcaccaatc aaggggcaga gacgctgcca tttggtaccg gctggcatcc
     5221 ttattttccg ttgtcaccac aaacgcggat tcaggcgcag gcgagcggtt actggctgga
     5281 acgggagcag tggctggcgg gtgagttttg cgagcagcta ccgcaggaac tggattttaa
     5341 ccagctcgcg ccgttgccgc gtcagtgggt gaacaatggt tttgccggat ggaatggtca
     5401 ggcccgcatc gagcagccgc aggaaggtta tgccatcatc atggaaacga cgccacctgc
     5461 accgtgttat ttcatctttg tttccgaccc tgcgtttgat aaaggatatg cgtttgattt
     5521 cttctgtctg gaaccgatga gccatgcgcc ggatgatcat catcgcccgg aagggggtga
     5581 tctcattgcg ctagcaccag gggaatcaac aacctcagag atgtcgttgc gggtagagtg
     5641 gctgtaattt ataaaatgcc ggataaggcg ttcacgccgc atccggcatc tttcctaacg
     5701 ccgatcgaga gaaattgccc ccggtccggt aattgccaat agcaagaacg cgccagcgat
     5761 actgacattc ttccagaaat taatcatatt tggcccaacc gcatcgccgg tcatatccca
     5821 gtaatggtga ccaatcaccg ccgtacccag cgtgtagaaa ataaacagca ccgccagcgg
     5881 acgggtgaaa aagccaagca cgattaatat cgcggcgggc acttccataa ctaccgcaat
     5941 aatcgctgcc agcatcggca ttggcgcgcc caacgaggcc atatattgga ccgtaccgtc
     6001 aaagcccatc attttgggaa aaccaaaaat aatgaaaatt aagaccacgg cgatacgggc
     6061 aattaataac aaaacggggc gggcagctcc aaaatcaaaa taacgtagtg tgttcataac
     6121 caatcccttt gcaagataat gtgatttaaa cgtaatacac actcttgcac ctcgccacgc
     6181 cccaggcatt gagatattcg ctaattatcc tggttacagt gatttaagcg cgatgttctc
     6241 atctatcagc agtttcgcat caaacgtttt gccgctctgg atccatttgc gaattgggcg
     6301 aatctcacgc ccctgattca gcgtgaccgc accgataagc acgccgtttt gcagattaaa
     6361 ccaaatcgcc ttctgagttt ctgggttgcc acgacaaagc tagtcatcgc cacgcatatc
     6421 gccaataaac tgtaagttat cactgtactg atcgctccag aaccacggcg gcggcagtag
     6481 cggtagcggt agccctaaca ttgcggcagc ggcaatttgc gcctggttat tggcgttttc
     6541 ccagctttcg cagcggtgta gtgcaccatt atcaagacga gtgattgcca catcgccacc
     6601 ggcaaagatc gcgggatcgc aggtgcggca agcctcatca atgacaatgc cattggcagt
     6661 atcaaggttg gcctcgcgag ccagttgctc gttggcgctg atactaatac cgtaaatcac
     6721 cacatcagcc tgaagcgttt ccccactttg cagcgtcagt tctacttttt caccatcgac
     6781 cacatgttca atggcattat tgagcagaat gcgcacacca gcctgctgat ggcgctgtaa
     6841 aagatagcgt tgcacgggcg gtggtgcatt acggcccatg acggttgccg ccagttcaat
     6901 cactgtcacc ttacatctgc gctgcgtggc gctggcagcc agttccagac caatagttcc
     6961 ggcaccgaca atcacgactg accgttcggg ctgcagaact tctcgcagtc tggcggcatc
     7021 accggcatgg tgtagggtaa agcagcgttc tcccagtgca tcaagcaacg gcagcggtcg
     7081 agctgccgcg ccggttgcta taaaaagctg atcccagtgc cagctttcgc cgttggttaa
     7141 cactaactct cgtgtgtcgc gacccagcgt tttgatggtt acaccggaat gcagatgaac
     7201 attgttttcc tgccaccagt tagcgggtaa cacctgctgt aactgcgggg aatcttccag
     7261 caacatggat ttcgagagtg gaggtcgttc ataaggaaga tgtcgctcat cggaaaacag
     7321 atgcagctca ccggtgaacc cttgctggcg tagcgaggcc gcagccattg ccgccgcttg
     7381 cccgccaccg acaataatga tcgttttttc tttcatcgtg cttccttaaa gatccagccc
     7441 agccgctacg tggcgaatgc cgcgaatcgc taaacccgca tcagcgttga tcatcacacc
     7501 gcttaatgcg cgattattgc gccgcgatgc caacatcaca tacggccccg taaaatccgc
     7561 cggttgcggg aaaaattgca gcggtaaaat ggcggcaatt ttctccggcg tcagagactg
     7621 cattatcgag gtttcacttt gcccgagcgc ctgtgggccg cgcaggtcgc tggccatacc
     7681 acacgggccg acgccattca cccgcacttt cggtgccagt tcataagcca gttggcgaat
     7741 aagtccggtt gcggcatgtt tactggtggt gtacagcggg ccaccgccgc caggatacca
     7801 ggcggcattt gacagtgtga aaatcatgct gccttcactg gcgattaacg ccggagcgca
     7861 ggcttttgcg cccagcaggt aaccgagtac gttgacgtta aacagctcgt ggaagccggt
     7921 ttcgagcgtc tctgcgggag tattaaccag tgaggcattg tgatcccaga tgcctgcatt
     7981 gccgataaaa caatccagct tgccggaacg agtcaggatc tgatcgaccg cgcgttgata
     8041 atcggcataa caggtcacgt taccttccac cgccagaata tgttcgccaa atcgctgacg
     8101 cagactggcg acttttgccg ccgacagttc cagcgcggca acctgcgcgc cttcttcgat
     8161 aaatcgctcg accagcgcca gccctaatcc cgatccgccg ccggtaataa aaatggactc
     8221 gttatgcaga tcgctcatgg ctgcgcctcc ggtaagtcga tgaaaatgtc accaccttca
     8281 acgtgtactg gataagtggt gagcggatcg gtggcgggca ggcataacgc tttccccgtt
     8341 ttcaggcaaa aactggcggc gtgtagcggg cactccaccg tggcgtcatc ttccagatac
     8401 ccttctgaca tcgacgcatt accatggctg caacgatcgt taatggcata aaactcgccg
     8461 cccacgttga acaaggcgat gacgggcgag gtatctatcc ggagagcctc accctccggc
     8521 acatccgcaa cgggacacgc ataaattcga ttcatcagaa cagtacactc aggttatggg
     8581 aagtgatcac cgcttgatcc aggacgatat cccgttccag caagcgccag ttgtcatctt
     8641 ccagacggcg aactttgtcg aaacgcgttc caacatagaa tgtttcatcg cgctcttttt
     8701 gtgcccgata aagtagataa ttcaccctca cggcaaatac gtttgggatg tcggtttcgc
     8761 ttacctggca gttgctgatt aagtgacgag tgcgtgacgg cggctcttct gcccaggcca
     8821 tgcccgtttc cagacgggcg attcgccgct ccagctggtc tttggtgtca ttgaaaatcc
     8881 aggtcgtcgg cggctggacg cctttgcgac ggtcgcgtgt ttgcgcgtta accgtggtgc
     8941 gcatggtgta acgaatctct tcgtcgagct gtgccaacca gtcacgaaat ttccagtcat
     9001 ccagcagact ggcttcgtga aagagaaact ggctaatgcg gtgatgtaac tccagtgaaa
     9061 cttgcgcact catttcatca cctcctgctg gtaagcggcg gttttatcga gcacttcctg
     9121 ccagctttcg ctactcagaa gatcggccca gcgttggtac attccacgag cggcagtttc
     9181 tgaaaagata tagttagtaa tgccaggaat gccgtcgtcg cggcgctttt cctgaccaag
     9241 ccccatttcc agacacagtt tgctgttgcg ggcgcggtgg cctttaagca atttctggat
     9301 ttcacaccag ttctccgagt catcctgctc gagaaaacca gcaggaccaa aagcacgagt
     9361 ggcgctgttt tcaaaagcgg ctttaacttc atcggaggcg gctttgtcag taatacagaa
     9421 cgcccacact tcaacttgat cagggccgcg cggatgccag acgcggagtg tggcagtgcc
     9481 gtttagccat gaaagcgtgg ggaatatatt gttatgaccc gccaggcgca gggcgcgaac
     9541 ttcacctaaa cgttgttctg cttcggcata ggtttcgcgg taatagcttg aaactgcgcc
     9601 atcgacccag acattagcat ccggtttttc agtaaagaag aaaccgctac cgtgaccgtc
     9661 ctgaccaaat tgcagcgcat ctttggcggt ttcccacacc gggcgggcgg tttgtccatc
     9721 accgaggcgc ttatcgctgc catcatcttt cgcccctaat acctgaacgg cagaagcatg
     9781 gctgaacaga gcatgatact ggtcactggc gaactgctct gccgggaatt tccagttaca
     9841 gttgatcacc cacttttgta cgccgccgac aatttcggtg ccgccttcgc gacgatccag
     9901 catgccatcc agataccagg caatgtcacc caggtaatca tgcaggcccg gtgcgctggt
     9961 atcccagttg ccaaaaatca gtcctttata actctccaca caaggaactt cgtttagccc
    10021 ccagtgggat ttacacaacc cttgtgggta ggcgcgaggt tccagcggta catcgatcaa
    10081 ctcgccgtta atgccataag accagccgtg atacgggcag gtaaaggcgc gagtgttgcc
    10141 acaatctgca taactcacac gcatggcccg gtggcggcat tggttaagaa acgccttgat
    10201 gctgccgtct ttctggcgca ccacgacaac tgcatcttct cccatgtagg tgttaaagaa
    10261 atcaccgggt tttgggatct ggctttcgtg ggcgagaaat agccagcaac gaccgaaaat
    10321 acgctcaagc tccagttggt aaatgtccgg gtcggtataa atacgcggag tgacccgacc
    10381 gttttgggtg tcaatcagtt ggtaaatgtt caaatctgag ggtgtggtca tattttttaa
    10441 tcctgaaaga taagatgtat tgaccttcta tttttgtgcc ataaggatgt tgctggttgg
    10501 ttgctaatat gtgaaatagt taatttctac cagctgagac gttttttcga taacaagggg
    10561 aaggtgatgg aactacggca tttacgctat ttcgtcgcag tggcgcaggc actgaacttt
    10621 acccgtgcgg cggaaaaact gcatacctca cagccttcgt taagcagcca gatccgcgat
    10681 cttgaaaact gtgtcggtgt tccgttactg gtgagggata agcgcaaagt cgcgttgacg
    10741 gcggcgggag aatgttttct ccaggacgcg ctggcaatcc tcgaacaagc ggaaaatgcc
    10801 aaattacggg cgcggaaaat tgttcaggaa gacagacaat taaccattgg ctttgtgcca
    10861 tcggcggaag tgaatttatt gccaaaagta ttaccgatgt ttcgtctcag acagccagac
    10921 accttgattg agctggtgag tttaatcacc acgcaacagg aggaaaaaat tcgccgtggt
    10981 gaactcgacg tcggcttaat gcgccatcct gtttatagcc cggagattga ttatctggag
    11041 ctttttgacg aaccattagt ggtggtgtta ccggttgatc accctttagc acatgaaaaa
    11101 gagatcaccg ccgcgcaact ggatggcgtg aatttcgtca gtaccgatcc ggcgtattcc
    11161 ggttcgcttg cgccgatcgt taaagcgtgg tttgcgcaag aaaacagcca gccaaatatc
    11221 gtccaggtgg caacgaatat tctggtgacc atgaatctgg tgggcatggg gctgggcgtc
    11281 actttgatac ccggttatat gaataatttt aataccgggc aggttgtttt tcgtcccatt
    11341 gccggaaatg tgccttccat tgctttactg atggcgtgga agaaagggga gatgaagccg
    11401 gcattacgcg atttcatcgc cattgtgcag gaacgtttgg caagcgtaac ggcataatta
    11461 cgcattattt ttgcaacatg cgccgcggat cgcagttttt tagtgcttgt gaactggcgt
    11521 tacagacatc gggaagcagg ctcgaatatt cttatttttc aacttgcacc aacattcgat
    11581 taaccccaga ctaacgcctc ctgacgggag ggactcatgg ttttgcaatc cacgcgctgg
    11641 ttggcgctcg gctatttcac atactttttt agttacggca tttttttacc tttctggagc
    11701 gtctggctta aagggattgg tttaacgcca gaaaccatcg gcctgttatt gggggcaggt
    11761 ctggttgccc gttttctcgg gagtttgctc atcgcgcccc gcgtcagcga tccttcccgc
    11821 ctgatttccg ccttgcgcgt gctggcactg ctgccacttc tctttgctgt cgccttctgg
    11881 gcgggggcgc acgtagcgtg gctgatgctg gtgatgattg gctttaacct ctttttctca
    11941 ccgctggtac cgttgaccga tgcactggcg aatacgtggc aaaagcagtt cccgcttgat
    12001 tacggcaaag tgcgactgtg gggctcggtg gcgtttgtca ttggctcggc gctgacgggc
    12061 aaactggtca ctatgtttga ttatcgggtg atcctcgtgc tgttgacgtt gggcgtggca
    12121 tccatgctgc tcggctttct catccgtccg acgattcagc cacaaggggc aagccgccag
    12181 caggagagca ccggttggtc tgcgtggttg gcgctggttc gccagaactg gcgctttctg
    12241 gcctgcgttt gtttattgca gggggcacat gcggcctatt acggttttag cgccatttac
    12301 tggcaggcag ctggctactc ggcctcggcg gtggggtatt tgtggtcgct gggcgtggtg
    12361 gcggaagtca ttatctttgc gctgagtaat aaacttttcc gccgttgtag tgctcgcgat
    12421 atgctgttga tctcggcgat ttgcggcgta gtgcgctggg gcattatggg agcaactacg
    12481 gcgttgccgt ggttgatagt ggtgcaaatt ctgcattgcg gcaccttcac ggtctgccac
    12541 ctggccgcca tgcgttatat tgctgctcgc caggttagcg aagtcatccg tttacaggcg
    12601 gtttactctg ccgtcgcgat gggcggcagt atcgctatca tgaccgtttt cgccggtttc
    12661 ctgtatcaat atctgggcca cggtgtgttc tgggtaatgg cgctggtggc gcttccggca
    12721 atgtttttgc gcccgaaagt tgttccctca tgctgattcg agcattttgc ggatctgctc
    12781 ctgttgaggt gttgtcagcg tcaggtccgt atagatcagc ggtggtgaaa agagcggtaa
    12841 cggcatggta taaggcgcaa taatcagtgc cgcgccacgc ggagcgcctg tctgcaaaaa
    12901 tgcctttacc gacatatgct taatgttgag cggcagtaac gttagttcgc gtaactgctg
    12961 ctcaatctgc gcttctcgct cgctatcatt cccggtcagt agaataatct gtttttcatg
    13021 cagatcgttt tcctgcatta gccaggcacc gaaaatcacc gcgaccagac cactttcctc
    13081 atcagaaaga tggacgccgt attcactttc aaatccggcc agcgccgcgc gggtgatgcg
    13141 taccaggcgt ggatacagtc tggcgaactc ttccggcagg gtattatcaa taccgatggc
    13201 aaacaaactg cgatttaacg cctgggcgag gtggatataa agctgatcac ataacccctg
    13261 ttcatcataa aaacgaacat ttcccagctc acgaaaatgg tttaccagac gtttgataga
    13321 ctgacgcaat tgtctgtccc gctgatgcgc atcccgcagt ggatcgggaa cccgcaacat
    13381 ggaaaaaagt aatgccataa acagtggctc atcaggcggt acatgctgga gcgcccgacg
    13441 ctgccagtgg cgtccaattt cctgcgctac ctgaaattca aggcaggatt ccgcccagcg
    13501 acgttggagc ggattaaact gaggcgtaat tccagcgtgg tgctgcagca aacaatattg
    13561 cagatacaga cacaggaaat gaatatcgcg cgattcaaag cgtttttgca gccgtcggga
    13621 gcagagattc actaacgctt gtagattggt atcgtcgtaa aagttacgcg caattccgcg
    13681 ccgcttcagt tcactcttca gggcgggggt gaattggctg gtaataaatg acggacacag
    13741 acggaaacca cgtcgtagcc agtgaaataa acacaaacgt tggttaagca ctgtaccttc
    13801 aacccggtag ctaccgtcat aaccagtcgt cagtgtgagt tgatgatagc gcaggatctc
    13861 ccgtcctgtt tcgctgatat caagactggc aatatcatca tccacaccat taagctcgct
    13921 gaaggttgcc atggtggcgg tcaaccccgg ctgaaagagc gtcagcaaga tctggcagcg
    13981 gcgctgggga gccgaaagga cagatggtgg agcaagcgta ggcatcatct gtagatctcc
    14041 cataagcata attgataaga atagctaaag tttgctcagg gaaatggcga agggaatgct
    14101 aatcatcaga aatgttggca agcgtcacag aatttttctc attttaaact gttctgacca
    14161 gtggattagc atgtcttaag gtggttttgt gaatgattat cgtgctagcg atcatgggtt
    14221 agttctgcgg attcaacgcg ctaaggataa agtccaccat ctgttcgcga taaacattgc
    14281 cgtgacgatc ggaaaaagca tcaatgtgtt cgccatccgg gattaggatc aaccgttttg
    14341 gctcttttgc caggctatac aacttttcgc tgtgctgcca tgggataacg tgatcggctt
    14401 taccgtgaat gagtaaaagc gggatcgggc tgacgctggc gatgtaattt tcgccgctgt
    14461 aactctcatc aagtaagtag ccactgccgg ggagcatctg gttggcgatg gttgcataag
    14521 aggcaaatgt ggagtcgagg atcaccgcac gtatgccttc acgatcaccc tgaccaataa
    14581 cagcaagaat attcgccccg ccaatactct gcccgaacag caccagccgt tgtgggttta
    14641 catcactgcg atggcgcacc acattgatgg cgctttgcgt atcgtccagc aatccggcct
    14701 gggacggagt gccttttgat ttaccaaacc cgcgataatc aaacataaaa acgttgaaat
    14761 tacgctcagg taaccaactg accagcggcc agtgggcgga catatttccg gcattgccgt
    14821 gagcatgaat gatggttgcg atggcgttgt cagcagggcc cgtcgaagaa ggaataaacc
    14881 agccttgcag acgagtaccg tccttagccg taaattcgac ggattccgcc gaccaggggt
    14941 cgggaccgta aattttatcg tcaggataat agaagaagtt gatggcaacg cgcagcacta
    15001 aataaacgca gaaggcaaca acaaagagaa taaacagaat tttgggaacg cgtttgttca
    15061 ctggcagtgc cataaccttt ccttgcgact tctttatcat ccattaatcg gggaaagatg
    15121 gtgggagagt gacgaaagaa agtaaagcgg tgaggaagta tttcggaata gcgcctgaaa
    15181 ggcgagggcg ggtgagtgat atcacccgcc tgagtcatta acgcttcaga gcgtcgctta
    15241 actcgtcacg catgttcgcc agtatggctt tcacaacacg cgggttacca gcaacgatgt
    15301 taccggtcag catatagtta tgaccaccgg tgaagtcgct gacgatgccg cccgcttcac
    15361 gaaccagcag atcgcctgcc gcgaaatccc acggacgcag accgatttca aagaaaccgt
    15421 caacacgacc cgcagcgacg taagccagat ccagcgccgc agaaccggta cgacggaagt
    15481 ctgcacattc gttgaacagc ttgccgacga tgttgatgta ggtagtggcg tactgttttg
    15541 ctttgaacgg gaagccggtc gccagaatgg taccgtcgag atcgcgagcg gtgctaccgc
    15601 gcagacggta gccgttcagc tgtgcgccct gaccgcgagt ggcggtgaac agttcgttac
    15661 gcataggatc gtataccaca gcaacttcgg tgcggccttt gatacgaacg gcgatagata
    15721 ccgcgaagtg cggcagacgt ttgataaagt tggtagtacc atccagtgga tcgataaccc
    15781 attgaacatc ctgatcagta ccttcaagtt caccgctttc ttcggtgatg atggtgtgct
    15841 gtgggtaaga tttacgaatc gtgtcgataa tcaccgcttc ggcagcttta tctacgttgg
    15901 tcacgaaatc gttactgcct ttctggctcg cttctacagc gtccggggtt tcatagtttt
    15961 tggcaattaa attacccgcc ttgcgcgctg cgcgcacggc gatgttcagc atcggatgca
    16021 tcagtctctc tcactggatg ttaaagaacg ggaaaacggc gcagagtata gcagcgagat
    16081 cggcatctgt cttcgtttta tgataagatg cgtgaataat ctttagatga agaaagacaa
    16141 tgctgcaaaa tattcgaatt gtgctggtgg agacgtcaca caccggcaat atgggttctg
    16201 ttgcccgtgc catgaaaaca atgggattaa ccaatctgtg gctggttaat ccactggtga
    16261 aacccgactc ccaggcgatt gccctggctg caggggccag cgatgtgatt ggtaatgctc
    16321 acatcgtcga tacgctcgac gaagcgttag ccggttgtag cctggtggtg ggcaccagcg
    16381 cacgctcccg cacgctgccg tggccgatgc tcgacccgcg cgaatgcggt ctgaaaagcg
    16441 tcgctgaagc ggcaaatacc ccggtggcgc tggtgtttgg tcgtgagcgc gtcggcttga
    16501 ccaatgaaga gttgcagaaa tgccattatc atgtcgcgat tgcggcaaac ccggaataca
    16561 gttcgctgaa cctggcaatg gcggttcagg ttattgccta tgaagtgcgt atggcctggc
    16621 tggcaactca ggaaaatggc gagcaggtcg agcatgaaga gacgccgtat ccgctggtcg
    16681 atgatctgga gcgtttttat ggtcatctgg aacaaacgct gctggcaacc ggttttatcc
    16741 gtgaaaacca tccggggcag gtgatgaata aattgcgccg tctgtttacc cgtgcgcgcc
    16801 cggaaagcca ggaattgaat atcctgcgcg ggattctggc ttctattgag cagcagaata
    16861 aaggtaacaa ggccgaataa cagtggttgc ctgatgcgac gcttaacgcg tcttatcagg
    16921 cctacaatgt acagaatcgt aggtcggata aggtgttcac accgcatccg acaacaggta
    16981 caaacgccac gataaaaaaa tggcactgaa ggttaaatac ccgactaaat cagtcaagta
    17041 aatagttgac caatttactc gggaatgtca gacttgaccc tgctatgcaa tacccccact
    17101 tttacaataa aaaaccccgg gcaggggcga gtttgaggtg aagtaagaca tgagactgac
    17161 atctaaaggg cgctatgccg tgaccgcaat gcttgacgtt gcgctcaact ctgaagcggg
    17221 cccggtaccg ttggctgata tttccgaacg tcagggaatt tccctttctt atctggaaca
    17281 actgttttcc cgtctgcgta aaaatggtct ggtttccagc gtacgtggac caggcggtgg
    17341 ttatctgtta ggcaaagatg ccagcagcat cgccgttggc gaagtgatta gcgccgttga
    17401 cgaatctgta gatgccaccc gttgtcaggg taaaggcggc tgccagggcg gcgataaatg
    17461 cctgacccac gcgctgtggc gtgatttgag cgaccgtctc accggttttc tcaacaacat
    17521 tactttaggc gaactggtta ataaccagga agtgctggat gtgtctggtc gtcagcatac
    17581 tcacgacgcg ccacgcaccc gcacacaaga cgcgatcgac gttaagttac gcgcttaata
    17641 aaaagaattc agaatcaggc cggagtgcta aatactccgt aaacacggtc gtacatccag
    17701 ccggtagcct gattccttgc attgagtgat gtacggagtt tatagagcaa tgaaattacc
    17761 gatttatctc gactactccg caaccacgcc ggtggacccg cgtgttgccg agaaaatgat
    17821 gcagttcatg acgatggacg gaacctttgg taacccggcc tctcgttctc accgtttcgg
    17881 ctggcaggct gaagaagcgg tagatatcgc ccgtaatcag attgccgatc tggtcggcgc
    17941 tgatccgcgt gaaatcgtct ttacctctgg tgcaaccgaa tctgacaacc tggcgatcaa
    18001 aggtgcagcc aacttttatc agaaaaaagg caagcacatc atcaccagca aaaccgaaca
    18061 caaagcggta ctggatactt gccgtcagct ggagcgcgaa ggtttcgaag tcacctacct
    18121 ggcaccgcag cgtaacggta ttatcgacct gaaagaactc gaagcggcga tgcgtgacga
    18181 caccatcctt gtttccatca tgcacgtaaa taacgaaatc ggcgtggtgc aggatatcgc
    18241 ggctatcggc gaaatgtgcc gtgctcgtgg cattatctat cacgttgatg caacccagag
    18301 cgtgggcaaa ctgccaatcg acctgagcca gttgaaagtt gacctgatgt ctttctccgg
    18361 tcacaaaatc tatggcccga aaggtatcgg tgcgctgtat gtgcgtcgta aaccgcgtgt
    18421 acgcatcgaa gcgcaaatgc acggcggcgg tcacgagcgc ggtatgcgtt ccggcactct
    18481 gcctgttcac cagatcgtcg gaatgggcga agcctatcgc atcgcaaaag aagagatggc
    18541 gaccgagatg gaacgtctgc gcggcctgcg taaccgtctg tggaacggca tcaaagatat
    18601 cgaagaagtt tacctgaacg gtgacctgga acacggtgcg ccgaacattc tcaacgtcag
    18661 cttcaactac gttgaaggtg agtcgctgat tatggcgctg aaagacctcg cagtttcttc
    18721 aggttccgcc tgtacgtcag caagcctcga accgtcctac gtgctgcgcg cgctggggct
    18781 gaacgacgag ctggcacata gctctatccg tttctcttta ggtcgtttta ctactgaaga
    18841 agagatcgac tacaccatcg agttagttcg taaatccatc ggtcgtctgc gtgacctttc
    18901 tccgctgtgg gaaatgtaca agcagggcgt ggatctgaac agcatcgaat gggctcatca
    18961 ttaatcggta tcggaatcag gagaatttat aatggcttac agcgaaaaag ttatcgacca
    19021 ttacgagaat ccgcgtaacg tgggttcctt tgataacaac gacgagaacg tcggcagcgg
    19081 catggtgggt gcaccggcct gtggcgacgt aatgaagttg cagattaaag tcaacgatga
    19141 aggtatcatt gaagacgcgc gttttaaaac ttacggctgc ggttccgcta tcgcttccag
    19201 ctccctggtc accgaatggg tgaaagggaa gtctctcgac gaagcgcagg cgatcaaaaa
    19261 caccgatatt gctgaagaac ttgaactgcc gccggtgaaa attcactgtt ctattctggc
    19321 agaagacgcg atcaaagccg ccattgcgga ctataaaagc aaacgtgaag caaaataaga
    19381 gttgaggttt ggttatgtcg attacactga gcgacagtgc agcagcgcga gtaaatacct
    19441 ttctggctaa ccgcggcaaa gggtttggcc tgcgtctggg cgtgagaacc tccggatgtt
    19501 caggtatggc ttatgtactg gaatttgttg acgaaccgac gccggaagac atcgtgtttg
    19561 aagacaaagg cgtgaaagtc gtggtcgatg gcaaaagcct gcaatttctg gacggtacgc
    19621 agctggactt cgtaaaagaa ggcctgaatg aagggtttaa attcaccaac ccgaacgtta
    19681 aagatgagtg tggttgcggc gaaagcttcc acgtttgatg cgcataccga taaccccacc
    19741 gtggtcgcct gcgcgtgggg tttgttttac ctgattcgcc gttatcgcgg cggatcgcag
    19801 ccctgagaat gttatggatt acttcaccct ctttggcttg cctgcccgtt atcaactcga
    19861 tacccaggca ctgagcctgc gttttcagga tctacaacgt cagtatcatc ctgataaatt
    19921 cgccagcgga agcctggcgg aacaactcgc cgccgtacag caatctgcaa ccattaacca
    19981 ggcctggcaa acgctgcgtc atccgttaat gcgcgcggaa tatttgcttt ctttacacgg
    20041 ttttgatctc gccagcgagc agcatactgt gcgcgacacc gcgttcctga tggaacagtt
    20101 ggagctgcgc gaagagctgg acgagatcga acaggcgaaa gatgaagcgc ggctggaaag
    20161 ctttatcaaa cgtgtgaaaa agatgtttga tacccgccat cagttgatgg ttgaacagtt
    20221 agacaacgag gcgtgggacg cggcggcgga tacggtgcgt aagctgcgtt ttctcgataa
    20281 actgcggagc agtgccgaac aactcgaaga aaaactgctc gatttttaat ttctggaagc
    20341 taaacatggc cttattacaa attagtgaac ctggtttgag tgctgcgccg catcagcgtc
    20401 gtctggcggc tggtattgac ctgggcacaa ccaactcgct ggtggcgaca gtgcgcagcg
    20461 gtcaggccga aacgttagcc gaccatgaag gccgtcacct gctgccatct gttgttcact
    20521 atcaacagca agggcattcg gtgggttatg acgcgcgtac taacgcagcg cttgacaccg
    20581 ccaacaccat tagttctgtt aaacgcctga tggggcgctc gctggctgat atccagcaac
    20641 gctatccgca tctgccttat caattccagg ccagcgaaaa cggcctgccg atgattgaaa
    20701 cggcggcggg gctactgaac ccggtgcgcg tttctgcgga catcctcaaa gcactggcgg
    20761 cgcgggcaac tgaagccctg gcaggcgagc tggatggtgt agttatcacc gttccggcgt
    20821 actttgacga tgcccagcgt cagggcacca aagacgcggc gcgtctggcg gggttgcatg
    20881 ttctgcgctt acttaacgaa ccgaccgctg cggctatcgc ctacgggctg gattccggtc
    20941 aggaaggcgt gatcgctgtt tatgacctcg gtggcgggac gtttgatatt tccattctgc
    21001 gcttaagtcg cggcgtgttt gaagtgctgg caaccggcgg tgattccgcg ctcggcggcg
    21061 atgatttcga ccatctgctg gcggattaca ttcgcgagca ggcggacatt cctgatcgta
    21121 gcgataaccg cgttcagcgt gaactgctgg atgccgccat tgcagccaaa atcgcgctga
    21181 gcgatgcgga ctccgtgacc gttaacgttg cgggctggca gggcgaaatc agccgtgaac
    21241 aattcaatga actgatcgcg ccactggtaa aacgaacctt actggcttgt cgtcgcgcgc
    21301 tgaaagacgc gggtgtagaa gctgacgaag tgctggaagt ggtgatggtg ggcggttcta
    21361 ctcgcgtgcc gctggtgcgt gaacgggtag gcgaattttt cggtcgtcca ccgctgactt
    21421 ccatcgaccc ggataaagtc gtcgctattg gcgcggcgat tcaggcggat attctggtgg
    21481 gtaacaagcc agacagcgaa atgctgctgc ttgatgtgat cccactgtcg ctgggcctcg
    21541 aaacgatggg cggcctggtg gagaaagtga ttccgcgtaa taccactatt ccggtggccc
    21601 gcgctcagga tttcaccacc tttaaagatg gtcagacggc gatgtctatc catgtaatgc
    21661 agggtgagcg cgaactggtg caggactgcc gctcactggc gcgttttgcg ctgcgtggta
    21721 ttccggcgct accggctggc ggtgcgcata ttcgcgtgac gttccaggtc gatgccgacg
    21781 gtcttttgag cgtgacggcg atggagaaat ccaccggcgt tgaggcgtct attcaggtca
    21841 aaccgtctta cggtctgacc gatagcgaaa tcgcttcgat gatcaaagac tcaatgagct
    21901 atgccgagca ggacgtaaaa gcccgtatgc tggcagaaca aaaagtagaa gcggcgcgtg
    21961 tgctggaaag tctgcacggc gcgctggctg ctgatgccgc gctgttaagc gccgcagagc
    22021 gtcaggtcat tgacaatgct gccgctcacc tgagtgaagt ggcgcagggc gatgatgttg
    22081 acgccatcga acaagcgatt aaaaacgtag acaaacaaac ccaggatttc gccgctcgcc
    22141 gcatggacca gtcggttcgt cgtgcgctga aaggccattc cgtggacgag gtttaatatg
    22201 ccaaagattg ttattttgcc tcatcaggac ctctgccctg atggcgctgt tctggaagct
    22261 aatagtggtg aaaccattct cgacgcagcg ctgcgtaacg gtatcgagat tgaacacgcc
    22321 tgtgaaaaat cctgtgcttg caccacctgc cactgcatcg ttcgtgaagg ttttgactca
    22381 ctgccggaaa gctcagagca ggaagacgac atgctggaca aagcctgggg actggagccg
    22441 gaaagccgtt taagctgcca ggcgcgcgtc accgacgaag atttagtggt cgaaatcccg
    22501 cgttacacta tcaaccatgc gcgtgagcat taacagaggt tagtatggga cttaagtgga
    22561 ccgatagccg cgaaattggc gaagcactgt acgatgcgta tcccgatctt gatccgaaaa
    22621 cggttcgatt caccgatatg catcagtgga tttgcgatct ggaagatttc gacgacgacc
    22681 cgcaggcatc caacgagaaa atcctcgaag cgattttgtt agtctggctg gacgaagccg
    22741 agtgaatgaa cgggctgcct gggggtagcc cgttttgctg ttaaacgaat aaggataact
    22801 aaaatgacag aagcgatgaa gattaccctc tctacccaac ctgccgacgc gcgctgggga
    22861 gaaaaagcaa cttacagcat taataatgac ggcattaccc tgcatttgaa cggggcagac
    22921 gatctggggc tgatccagcg tgcggcccgc aagattgacg gtctgggcat caagcatgtt
    22981 cagttaagcg gtgaaggctg ggatgcggat cgctgctggg cattctggca aggttataaa
    23041 ggcccgaaag gcacgcgtaa agtggagtgg ccggatctgg acgatgccca gcgccaggaa
    23101 ctggataacc gcctgatgat catcgactgg gtgcgtgaca ccatcaacgc accggcagaa
    23161 gaattgggac catcgcaact ggcacagcgt gctgttgatc tgatcagcaa cgtcgcgggc
    23221 gatcgtgtga cttatcggat caccaaaggc gaagatctgc gtgagcaagg ttatatgggg
    23281 ctgcacactg tcgggcgcgg ttcagaacgt tctccggtat tgctggcgct ggattacaac
    23341 ccaactggcg ataaagaagc accggtgtac gcgtgcctgg taggtaaagg tatcactttt
    23401 gactccggcg gctacagcat caaacagact gcgtttatgg actcgatgaa gtcggacatg
    23461 ggcggcgcgg caacggttac cggggcgctg gcatttgcca ttacgcgcgg actgaacaag
    23521 cgcgtgaagc tgttcctctg ctgtgcggat aacctgatta gcggcaatgc gttcaagctg
    23581 ggcgatatca tcacttatcg caacggtaaa agagttgaag tgatgaacac tgatgccgaa
    23641 gggcgtctgg tgcttgccga tggtctgatt gatgccagtg cgcagaaacc ggaaatgatc
    23701 attgatgcgg cgaccctcac cggggcggcg aaaactgcgc tgggtaatga ttatcacgcg
    23761 ctgttcagtt ttgacgatgc gcttgccggt cgtttactgg cgagtgcctc acaagagaac
    23821 gaaccattct ggcgtctgcc gctggcggaa ttccaccgca gccagctgcc gtctaacttt
    23881 gccgaactga acaataccgg aagcgcggcg tatccggcag gcgcgagcac ggcggcgggc
    23941 ttcctgtcgc actttgttga gaactatcag caaggctggc tgcatatcga ctgctcggcg
    24001 acttaccgta aagcgccggt tgaacagtgg tctgcgggcg ctacgggact tggtgtgcgc
    24061 acgatagcta atctgttaac ggcgtaaact aatatgccgg atgcggcgta aacgccttat
    24121 ccggcctaca aattctgcaa attcaacaaa ttgcgaatcc cttgtaggcc tgataagcgt
    24181 agcgcatcag gcatttgcgc cccgaacgtt gaatgaattg tggttcttat gtccgaaaca
    24241 aaaaacgaac ttgaagacct gctggaaaaa gcagcaaccg aaccggcgca ccgcccggcc
    24301 tttttccgta ctctactgga atccaccgtc tgggtgcctg gtacggcggc gcagggcgag
    24361 gctgtggttg aagattgtgc gcttgattta cagcactggg aaaaagaaga cggcaccagc
    24421 gtcattcctt ttttcacctc gttagaagcg cttcaacagg cggttgaaga cgaacaggca
    24481 tttgtcgtaa tgcccgttcg cacattgttt gagatgaccc ttggcgaaac gctcttcctt
    24541 aatgccaaac tgccgaccgg taaagaattt atgccgcgtg aaatcagttt gttgattggt
    24601 gaagagggaa atccgctgag cagccaggaa atcctggaag gcggtgaatc gctgatatta
    24661 tcggaagtcg cagagccgcc agcacaaatg attgattcac tcaccacctt atttaaaacc
    24721 attaagccgg tgaagcgtgc ttttatttgt tcaattaaag agaacgaaga ggcacagcct
    24781 aatttactta ttggcattga agccgatggt gatatcgaag aaattattca ggcgacggga
    24841 agtgtagcga ccgatacatt acctggcgat gaaccaatcg atatttgtca ggtgaaaaaa
    24901 ggggaaaaag gaattagcca ctttattacc gaacatattg cgccattcta tgaacgtcgc
    24961 tggggtggtt ttttgcgtga ctttaaacaa aaccggataa tctaaaatat gccgccctaa
    25021 agggcggcat tcgggcttac ttgctgctct gaaggttgtt aaccaccagt aaattgtcgc
    25081 cggagagact ccggttatta tttacttggg aaaacagcgc accagaaaca ataagtacga
    25141 tacagaagca ccgtatcttc attgtgctgt cgtgatgcga agaccgcatc acgacagcac
    25201 cactggcgat attgccgcga tagcaagcgc tgagacgcgc ttgctctaac ggtcgcggaa
    25261 catcctttcc ttaagccagc gcccgcttgc gagacggcgg aaagggccgc agtggataat
    25321 acactaacag aacaataata actctctttt gcttgacaaa aagagagtta ctggcgagta
    25381 ttgtctccgc tgagacgcgc ttgcattact acggtaatac aacctgctgg aaaggccgcg
    25441 aaccagacca gcaataaaaa aacgccaaat tcggcggttt tttattgcct gtaaaatact
    25501 tatttcactg gctcaaccgg taaatctgcc cgcgcgcccc attcactcca tgcgccgtcg
    25561 tacagtttca cgtttggcac atccagcgtc gcgagtgcta acaaaaccac ggctgccgtt
    25621 acaccagagc cgcagctgac gataatcggt ttgtcatagc tgacgccgcg accaaaaaat
    25681 atcgcatcca gttcatcggt cgtttttagt tcgccttcgc gcaccagttc cgtccacgga
    25741 acgttcaatg ctccgggaat atgtccgcga cgtaaacctg ggcgaggttc atcaacttct
    25801 gcgttaaaac gtgcagccgg gcgggcatca ataatttgcg ccgtattttc atggcttgcc
    25861 aataacacat cggttacttt caccactgct tcaggattaa acgcggcgtt aaactctcct
    25921 tccggcagct ctactgcacc ttcttctaat agcaggtcat cgcgctgcca gcctgcaag
//
LOCUS       DAJTXY010000045        25889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTXY010000045 DAJTXY010000000
VERSION     DAJTXY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.045"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            347..640
                     /locus_tag="OO566_002002"
     CDS             347..640
                     /locus_tag="OO566_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6605300.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="OO566_002003"
     CDS             712..1371
                     /locus_tag="OO566_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6605301.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1463..1909
                     /locus_tag="OO566_002004"
     CDS             1463..1909
                     /locus_tag="OO566_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6605302.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="OO566_002005"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="OO566_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /locus_tag="OO566_002006"
     CDS             3389..3808
                     /locus_tag="OO566_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6605303.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQRL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5074
                     /gene="umuC"
                     /locus_tag="OO566_002007"
                     /pseudo
     CDS             3808..5074
                     /gene="umuC"
                     /locus_tag="OO566_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(5120..5650)
                     /gene="dsbB"
                     /locus_tag="OO566_002008"
     CDS             complement(5120..5650)
                     /gene="dsbB"
                     /locus_tag="OO566_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6605304.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5796..7337)
                     /gene="nhaB"
                     /locus_tag="OO566_002009"
     CDS             complement(5796..7337)
                     /gene="nhaB"
                     /locus_tag="OO566_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6605305.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7558..8277
                     /gene="fadR"
                     /locus_tag="OO566_002010"
     CDS             7558..8277
                     /gene="fadR"
                     /locus_tag="OO566_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6605306.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8329..9861)
                     /locus_tag="OO566_002011"
     CDS             complement(8329..9861)
                     /locus_tag="OO566_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6605307.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHTCYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10191..11489
                     /gene="dadA"
                     /locus_tag="OO566_002012"
     CDS             10191..11489
                     /gene="dadA"
                     /locus_tag="OO566_002012"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6605308.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11499..12569
                     /gene="dadX"
                     /locus_tag="OO566_002013"
     CDS             11499..12569
                     /gene="dadX"
                     /locus_tag="OO566_002013"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6605309.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12737..14163)
                     /locus_tag="OO566_002014"
                     /pseudo
     CDS             complement(12737..14163)
                     /locus_tag="OO566_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14773..16509)
                     /locus_tag="OO566_002015"
     CDS             complement(14773..16509)
                     /locus_tag="OO566_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6605310.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16604..17518)
                     /gene="ldcA"
                     /locus_tag="OO566_002016"
     CDS             complement(16604..17518)
                     /gene="ldcA"
                     /locus_tag="OO566_002016"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6605311.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17618..18229
                     /gene="emtA"
                     /locus_tag="OO566_002017"
     CDS             17618..18229
                     /gene="emtA"
                     /locus_tag="OO566_002017"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6605312.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18231..18965)
                     /locus_tag="OO566_002018"
     CDS             complement(18231..18965)
                     /locus_tag="OO566_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6605313.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19166..19420
                     /locus_tag="OO566_002019"
     CDS             19166..19420
                     /locus_tag="OO566_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6605314.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19470..20930)
                     /locus_tag="OO566_002020"
     CDS             complement(19470..20930)
                     /locus_tag="OO566_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6605315.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20867..21424)
                     /locus_tag="OO566_002021"
     CDS             complement(20867..21424)
                     /locus_tag="OO566_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6605316.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARVLTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21450..22304)
                     /locus_tag="OO566_002022"
     CDS             complement(21450..22304)
                     /locus_tag="OO566_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6605317.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22304..23113)
                     /locus_tag="OO566_002023"
     CDS             complement(22304..23113)
                     /locus_tag="OO566_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6605318.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23123..23881)
                     /locus_tag="OO566_002024"
     CDS             complement(23123..23881)
                     /locus_tag="OO566_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6605319.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23878..24858)
                     /locus_tag="OO566_002025"
     CDS             complement(23878..24858)
                     /locus_tag="OO566_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6605320.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISP
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24858..25880)
                     /locus_tag="OO566_002026"
     CDS             complement(24858..25880)
                     /locus_tag="OO566_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6605321.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6450 a         6372 c         6727 g         6340 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgtgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcctgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct tcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagact actgtatata
     3361 aaaacagtat agcttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaacggttg atccagcatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta ccggtggttg tgctatcgaa
     3901 taatgacggt tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggcg ttaaaatggg
     3961 cgatccctgg ttcaaacaaa aagatctgtt tcgtcgctgt ggtgtggttt gctttagcag
     4021 caattatgag ctttacgcag acatgagcaa tcgggtgatg tcgacgctgg aagaactatc
     4081 gccccgcgtc gagatttaca gtattgatga agcattctgc gatctgacag gtgtgcgtaa
     4141 ttgtcgcgat ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca
     4201 tcttactgtt ggtgtgggga tcgcccagac caaaacgttg gctaagcttg ccaatcatgc
     4261 ggcaaaaaaa tggcagcggc agacgggtgg ggtggtggat ttatcaaatc tggaacgcca
     4321 gcgtaaatta atgtctgctc tccccgtgga tgacgtctgg gggattggac ggcggatcag
     4381 caaaaaactg gacgcgatgg ggatcaaaac cgttctcgat ttggcggata cagatatccg
     4441 gtttatccgt aaacatttta atgtcatgct cgaaagaacg gtgcgtgaac tgcgcggcga
     4501 accctgtttg caactggaag agtttgcacc gacgaagcag gaaattatct gttcccgctc
     4561 gtttggtgaa cgcatcacgg attatacgtc gatgcggcag gccatttgta gttacgctgc
     4621 ccgggcggcg gaaaaacttc gcagtgagca tcaatattgt cggtttatct ccacatttat
     4681 taagacgtca ccatttgcgc tcaatgaacc ttattacggc aatagcacgt cggtaaaact
     4741 gctgacgccc actcaggaca gcagggatat cattaacgct gctacgcgat ctctggatgc
     4801 catctggcaa gcgggccatc gctaccaaaa agcgggcgtg atgctggggg atttcttcag
     4861 tcagggagtc gcgcagctca atttattcga tgacaatgca ccgcgccccg ggagtgagca
     4921 attgatggcg gtaatggata ctctgaatgc taaagagggc agaggaacac tctattttgc
     4981 cgggcagggg atccaacagc aatggcagat gaagcgagct atgctttcac cacgttatac
     5041 aacgcgaagt tctgatttac tgcgggtcaa ataaatatag cggcaggaaa aaagcgttcc
     5101 cgcaggagcg ccgaatggat tagcgaccga acagatcacg ttttttcgct ttaaacggct
     5161 gggaaatcac caccagcacc gcgacaatca ggtaagcgat aaaaataccg agcagccact
     5221 gcggcatttc catacctaaa aaatcccact gacgctcggc gcaatcgcca gaggcgacaa
     5281 acacttgcgg cacccattta tccagcggca gccattccgg gaaacgaacc ataaaatcac
     5341 aggtggcaaa tggcgaagga tagagctgaa gcatggtgtg ctcgtaagtt aactgcacac
     5401 cgcggaacgc actatacaac cagataacca tcgctacata acgcagcgga gttttcgggg
     5461 cgatcgcgcc aatcagcgca gcacccagaa cgccgaataa cgcgcagcgt tcataaatac
     5521 agagcacgca aggtttcagt aacatcacat gctggaacca cagcgccgtc agttccagtg
     5581 ccagagcagt aaacgccatc aacagccacg cgccccggcc ttgtgaacat tggttcaaaa
     5641 atcgcaacat aatcatttcc ctgcaatatg catagagtgc gcagtttaaa ccaattcatt
     5701 cgctgcgcca ccaggaggca gcaaaaatag gacgcaattg gacaattatc gggcgaaaag
     5761 gcaaaccggg catttatatg cccggtaagt tgttatcaaa gcgttgctat ccagcccatt
     5821 tgcataaacc attcggttac aggggcaagc gtaaactcga cgcagagcaa gccgacgagt
     5881 gtcagtacga gggtgtaagg cagggccatc cacaccatgc ggccataaga gaggcgaatc
     5941 aatggcgcga gtgcagaggt cagcaggaac aggaacgcag cctgaccgtt cggcgtagcg
     6001 acagagggca gattggtacc ggtattaatg gcgaccgcca gcagctcgta ttgcttcaac
     6061 gtgatagcgc cactttccat tgccgctttc gcttcgttga tataaatcgt ccccacgaag
     6121 acgttatccg aaatggatga cagcagaccg ttgaaaatat agaacagcga cagctgagca
     6181 tgttcagatg cctgcaacac aaactgaata atcggcgaaa acagttgttg gtcgataatc
     6241 accgcgacta ccgagaaaaa caccgtcaac agtgcggtga atggcagaga ttcggtgaag
     6301 gctttaccga tagcatgttc atcggtgaca ccggtcaatg atgttgccag aataatgact
     6361 gacaaaccaa tcaagccaac ttccgccaga tgcaacgcca gcgcagtcac cagccagacg
     6421 ccaataatcg cctggacaat cagacggatt ttatcctgac gggtacgctg gtggcggctt
     6481 tgatcgtcaa actgttgcaa cacttcgcgg actttctccg gcagcgtttc accgtagcca
     6541 aaccaacgca gcttctctac cagcaggcag gttaacaggc cacaaatcag aaccggaacg
     6601 gtcaccggcg acatgcgcag gaagaaatcg ccaaaatgcc agccagccgc tttagcgatg
     6661 atcaggttct gtggttcgcc caccatggtc attacgccgc ctaatgcggt gccgacaccg
     6721 gcatgcatca tcaggctacg cagaaagcca cggaactgtt ccagaaccac gttgtaatgc
     6781 ttgtcgatat gactatcgtc ttgcaggtcg gtgtcttcgg tacgggaaga ggctacgcga
     6841 tgataaatac cataaaaacc gactgcaacg ctgatcacca ccgccacgac ggttaaggca
     6901 tcgaggaacg cggagaggaa cgctgccgcc acgcaaaaag agagcgacag cagcattttg
     6961 gagcgaatgc ttaacagcaa acgggtaaat atgaacagca acagctgttt cataaaatag
     7021 atacccgcca ccataaacat caataacagc aagacttcaa gatttgccgc caccccttca
     7081 cggacgtgtt ccgcgctggt catgccgatg aataccgctt cgatagccaa cagaccaccg
     7141 gggagtagcg ggtagcattt cagggccatc gccagagtga aaataaattc cgctaccagc
     7201 aaccagcccg cgacgaaagg gctgatgagg aaaattaacg ggtttacgat taagaaaatg
     7261 atgagggcga gtttgtacca gtcgggggac tggcccaaaa aattgcgcca tagcgcgcgg
     7321 ccccaggaga tctccatgat ggtttccctt accttacaaa taatcaatga tgtttttgtg
     7381 tttaaacgca aagcttaacg gtcaggcagg agtgaggcaa gtcttgatag tcaaggggaa
     7441 agagatgcgg aaaatgaagc cttgatccct ttttcttctt tttgtctgct atcagcgtag
     7501 ttagccctct ggtatgatga gtccaacttt gttttgctgt gttatggaaa tctcactatg
     7561 gtcattaagg cgcaaagccc ggcgggtttc gcggaagagt acattattga aagtatctgg
     7621 aataaccgct tccctcccgg gactattttg cccgcagaac gtgaactttc agaattaatt
     7681 ggcgtaacgc gtactacgtt acgtgaagtg ttacagcgtc tggcacgaga tggctggttg
     7741 accattcaac atggcaagcc gacgaaggtg aataatttct gggaaacttc cggtttaaat
     7801 atccttgaaa cactggcgcg actggatcac gaaagtgtgc cgcagcttat tgataatttg
     7861 ctgtcggtgc gtaccaatat ttccactatt tttattcgca ccgcgtttcg tcagcatccc
     7921 gataaagcgc aggaagtgct ggctaccgct aatgaagtgg ccgatcacgc cgatgccttt
     7981 gccgagctgg attacaacat attccgcggc ctggcgtttg cttccggcaa cccgatttac
     8041 ggtctgatcc ttaacgggat gaaagggctg tatacgcgca ttggtcgtca ctatttcgcc
     8101 aatccggaag cgcgcagtct ggcgctgggc ttctaccaca aactgtcggc gttgtgcagt
     8161 gaaggcgcgc acgatcaggt gtacgaaaca gtgcgtcgct atgggcatga gagtggcgag
     8221 atttggcacc ggatgcagaa aaatctgccg ggtgatttag ccattcaggg gcgataatcc
     8281 cttccgttta aagagcaaac ccctcaaacg aggggttttt tgttgttttt acagatttcc
     8341 cattcttggc gggcaacgtt ccagcaactc gatgctgccg tcttcgtttt gctgttcgag
     8401 catcacatca aatccccaca ggcgatgcac atgcttcagg acttctttgc gcccccgatc
     8461 cagcggtgcg cgattatgtg gaatataacg cagcgtcagc gaacggtcgc cgcgcaaatc
     8521 cacgttccag atctgaatat tcggctccag attacttaag ttatattgcg acgataaccg
     8581 gttacggatc tcccgataac cttcttcatt atgaatagcg gaaatctcca gataattatg
     8641 ccgatcatca tccagcacgg tgaagaagcg gaaatcacgc atcactttcg gtgacagaaa
     8701 ctggctgata aaactctcat ctttgaaatc acgcatcgcg aaatgcagcg tttccagcca
     8761 gtcggaaccg gcgatatccg ggaaccagta tttgtcttct tccgttggcg actgacaaat
     8821 ccgtttaata tcctggaaca tggcgaaccc gagggcatac gggttgatgc cgctgtacca
     8881 cgggctgtta taggggggct ggaagaccac attggtgtgg ctgtgcaaaa actccagcat
     8941 aaaacgttcc gttactttcc cttcatcata cagatggtta aggatggtat agtgccagaa
     9001 ggtcgcacag ccttcgttca taacctgagt ctgtttttgc ggataaaaat actggctcac
     9061 cttacgcaca atacgcagga tttcacgctg ccatgattcc agcagtggcg catttttctc
     9121 cataaaatag agcaggtttt cttgtggttc ggacggatag cggcgcgctt cagcaaccgt
     9181 tttctcttcc tcgcgcttcg gcagggtacg ccagagcata ttgacctgac tttgtagata
     9241 ctcttcgcga cttttctgcc gggctttctc ttcttgcagc gagatttttt gcgggcgttt
     9301 gtaccggtcc acgccgtagt tcatcagcgc gtggcacgag tccagaagcc gttctacttc
     9361 atcaacgcca taacgttctt cgcactcggt aatatattta cgggcaaaaa tcagataatc
     9421 gacaatcgaa ctggcgtcgg tccaactgcg gaataagcaa ttgtttttaa agaaagagtt
     9481 atgcccatag caggtgtgtg ccatcaccag cgcctgcata gtaatggtgt tctcttccat
     9541 caagtaagcg atacacgggt tagagttaat gacgatttca taggccagcc cttgctgacc
     9601 gtgcttatac agccgttcag tctcgataaa ctttttaccg aatgaccagt gcggatagtt
     9661 aattggcatg ccgacgctgg aataggcatc catcatctgt tctgaggtta tcacttcaat
     9721 ctggtgcggg taagtatcca gccggtagag tttcgccacc cggtctatct ctgccagata
     9781 aacatccagc aggtcgaacg tccagtcggg tccatcgctc aaacgtgtgg tgtccttatt
     9841 aatagaatcg atcgtcgcca tacgcgcacc tcattgttgt cggcgctctc tgtgtggagc
     9901 acctcatttc aagcatagaa cacctgttaa aaaccgcgtc gccggagaat ttttttcttt
     9961 gcgatttctt attatcagag tgccactaat ccgcttctga acggaatttt atgctggata
    10021 aaaagggcgt tcagcaggag atactaaaga cgccatattg ccgcagagtc agggagatgt
    10081 gagtcagctc accataaaaa agccgtatgt tgaataatat tttcaactga gttatcaaga
    10141 tgtaattaga ttattattct tttactgtat ctaccgttat cggagtggct atgcgagttg
    10201 tcatactggg aagtggtgtg gtaggcgttg ccagcgcctg gtacttaaat caggcaggac
    10261 atgaggtcac cgtcattgat cgggagccgg gggccgccct ggaaaccagt gccgcgaatg
    10321 ccgggcaaat ctcccccgga tatgctgcac cgtgggcagc accaggtgtg cctttaaaag
    10381 cgattaaatg gatgttccag cgccatgcgc cgctggcggt tcgtctcgac ggtacgcagt
    10441 ttcagttgaa atggatgtgg caaatgttac gtaactgcga caccagccac tacatggaaa
    10501 acaaagggcg gatggtgcgt ctggcggaat acagccgtga ttgcctgaaa gcattgcgcg
    10561 ccgaaaccaa tattcagtat gaagggcgtc agggtgggac gctgcaactg ttccgtaccg
    10621 aacaacagta tgaaaatgcg acccgcgata tcgccgtgct ggaagatgcc ggcgtaccgt
    10681 atcagctgct ggaatccagc cgcctggcgg aagtggatcc cgcgctggca gaagtagcgc
    10741 acaaactgac gggcggcctg cagttaccca atgatgaaac cggagactgc cagctgttta
    10801 cccagaatct ggcgcggatg gcagagcagg cgggggttaa attccgcttt aatacgcccg
    10861 ttgaccaact gctttgcgac ggcgagcaaa tctacggcgt gaagtttggc gatgaagtga
    10921 ttaaggccga tgcgtatgtg atggcgtttg gttcttactc gacggcgatg ctcaaaggca
    10981 ttgttgatat tccggtgtat ccgctgaaag gctactcgct gactattcca attgcgcagg
    11041 aagatggtgc gccagtatcc accattcttg atgaaaccta caaaatcgcc attacccgtt
    11101 tcgataaccg cattcgtgtt ggcggaatgg cggaaattgt tggttttaac accgagctgt
    11161 tgcaaccgcg tcgtgaaacg ctggaaatgg tggttcgcga tctctatcca cgcggcggtc
    11221 atgtcgagca ggcgactttc tggacgggtt tgcgcccgat gacgccagac ggtacgccgg
    11281 ttgtcgggcg tacacgcttt aaaaatctgt ggctgaatac cggtcacggc acgctcggct
    11341 ggacgatggc ttgcggttcc ggtcagttgt taagcgatct gctctctggt cgcacgccag
    11401 cgatcccata tgaggatcta agcgtagcgc gctacagccg tggatttacg ccatcacgtc
    11461 cgggccattt acatggcgca cacagctaag gaaacgagat gacccgtccg atacaggcca
    11521 gcctcgatct gcaggcatta aaacagaatc tgtccattgt ccgccaggcc gcgccgcacg
    11581 cgcgcgtctg gtcggtggta aaagcgaacg cttacgggca tggtattgag cgtatctgga
    11641 gcgcgctcgg ggccaccgat ggctttgcat tacttaacct ggaagaggca ataacgttac
    11701 gtgagcgcgg ctggaagggg ccgatcctga tgctggaagg atttttccat gctcaggatc
    11761 tggagattta tgaccagcac cgcctgacca cctgcgtaca cagcaactgg cagctcaaag
    11821 cactgcaaaa tgcgcggcta aaagcaccgt tggatattta tcttaaagtg aacagtggga
    11881 tgaatcggtt gggcttccag cccgatcgcg tgcttaccgt ctggcagcag ttgcgggcaa
    11941 tggcgaatgt tggcgaaatg accctgatgt cgcattttgc tgaagcggaa catcctgatg
    12001 gaatttccgg cgcgatggcg cgtattgagc aggcggcgga ggggctggag tgtcggcgtt
    12061 cgttgtccaa ttcggcggcg actctgtggc acccggaagc gcattttgac tgggttcggc
    12121 ctggcattat tttgtatggc gcttcgccgt ccggtcagtg gcgtgatatc gccaataccg
    12181 gattacgtcc ggtgatgacg ctaagcagtg agattattgg tgtccagacg ctaaaagcgg
    12241 gcgagcgtgt gggctacggc ggtcgctata ctgcgcgcga tgaacagcga atcggcattg
    12301 tcgccgcagg atacgccgac ggttatccgc gccatgcgcc taccggtact cctgttttag
    12361 tggacggcgt gcgcaccatg acggtgggga ccgtctcgat ggatatgctg gcggttgatt
    12421 taacgccttg cccgcaggcg gggattggta cgccggttga gctgtggggc aaggagatca
    12481 aaattgatga tgtcgccgcc gctgccggaa cggtgggcta tgagttgatg tgcgcgctgg
    12541 cgttacgcgt cccggttgtg acagtgtaac ttgttgtaag ccggatcgga ggcagcgtct
    12601 tctggttgcg aaaaaatcat ccatccggct ggtcagcaac tgtagttgtt aatgtgacag
    12661 agccattgcc catgatagtg tccattaaaa gaatggacac tatttccccg gaacctgaac
    12721 tcgccgcaca ggcgttctac ataaaacgct tacgcttcat tgttgactcg gagtcgactt
    12781 agataaatca cgcttcaccc ttgatgcaga ttcgggttca tgttcagatt gttgattaac
    12841 aggctgcgag tcctgttgtc tttccagact caacaggccc aactgttgat ccaggagctc
    12901 caggtcctcc tgtaccttca cgcgctccag gagttccggg tcatccagga actccagatc
    12961 cataaggtcc tccagctcct cgagtccttc caggtcaacc tagccttcct tgtgcaaaaa
    13021 gtccaaaagg tccttcaggc cctgctgttt tttcaggccc tcgacggctt tgagcagcgt
    13081 cggtaaattg tctttgtccc acgggttacc attacagtca aacaccttca ggctctgggg
    13141 ctgcggcacc tgtaccgttt taaacttgtt ttctccacag tagtaaatta ttagtttggg
    13201 cggcagccgt ggtgaagaga tcaaatcgtt actagaacag tcaaatactt tcaggcgagt
    13261 caagttctcc agcatctcat caggtattga gttaatattg ttgccggcac aggatagttt
    13321 atacaggtac tggggcatcg acggcggtaa aaattgcaag aagttttcag aacagttaat
    13381 cactctcagg ttagtcgaca gctcaggcag ccagataata cagttttcat agcacttcag
    13441 taccatcagt tttgagggct gcaccttcgg taacaagatc aagacgttat ctgtacagtc
    13501 aagcaccagc agccactttg gcatcacctt cggtaacgcg gtcaacacgt ttttactgca
    13561 gtaaaattca atcagatgtg gaggcaaatt ctcagggagt gaagtcaatc ccaggttgct
    13621 aagattgagc atactccgtc ttgtaatgag acattccttc attctgcgca atgcttctgc
    13681 ccggttttca atccggcttg ctggcaggtt ttcttccagg atattttcct tgatttcttc
    13741 cagactttct ttcaggtctt cttggggaat ttctgactgt ttgttttgct ggttttcttc
    13801 caggatttcg tacaggtttt tttgcaggtt ttcttgcaaa ccatcctgct gtatagccca
    13861 ttcgcaccat ttggcgacca tcatgaactc gttatgttgc tgattcaggc gagtccccaa
    13921 cgtgatgatg gtattgttgt ccacctgtat gttcactttg cccgcgatac tgaggtcaat
    13981 gatctgccag ggggcatcac cccagctgaa tttttgcggg ctgagatact tcatgttctg
    14041 caaaatttga ttcttgagtt ccaccgacag gaagagccgc cctggcgggc gtcgtacgtc
    14101 tgggttaacg gaagatgggg gaatggcggg ggcgaatggg cagatgtctt taaagctagc
    14161 cataatgcta cgtttccttg aaataaatat cctctggcag cgccggagac gtgaatgtgg
    14221 tcaccgcaaa ggagccacag gacgtcattt gcgctgatat cactactcat cgttgacttt
    14281 ttccctggtc agaaatgccg tgtcgggtct ggtacatgct gtattacggc ctaggtagct
    14341 ctgacgtatt aaagaaaata cgagagtgaa cgacccggaa ataatcgtta ttaagcaaag
    14401 gaataactat aagaaatcgc tcatgcatcc ggtgctacag gcggatgaaa cgcctattgt
    14461 gtgggctatg tcagtgcggc aggttgcgtg gggaatatgt tcaggcgatg tcaggcaggg
    14521 gcccctgatg gttgaccggt aatgcggggc tggtttttat tacctgttac agtcggaaaa
    14581 agcccttacg ctgactttct tgagccggtg ttttgagttc attagccaga gcctgcaatg
    14641 gtttgtcatc cataatttaa ccttcttctg atgttgggat gagcatatca aaatcaggaa
    14701 atgacaagtt tattacaggc tcaggctgcg ttcagtaaag catcgcatcc ggcgatagat
    14761 gctgcattaa atttaagatt cagcttcctc ttcagccacc cgaacgccaa tcttcaacac
    14821 ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta cctggtcacc
    14881 gacaaccggt gccgcgccta gcaactgctg gacaatttca cccagcgttt gctgtttatc
    14941 acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat atttggcgct
    15001 ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg gcgactggct
    15061 gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca atacatcgcc
    15121 ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa acagggctgc
    15181 aatacgcgtc tcttttggca tatgcaaatc gcgcagtgcc gcacctacac accatttatc
    15241 agcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat ccaggccaac
    15301 gcgtgacacc ggacgtccga cgggcggaac caccactttg gcttttttcg ccgcccacga
    15361 gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga cattaaagaa
    15421 cagtcgcgca ttctccagcc ccgccatcat cgggaacacg gccaggatga ttggcaccgc
    15481 gccgcgtaat cccacccagc tgataaacac gcgctcacgc agattgaaac cgcggaaagg
    15541 gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg ccgacaaaat
    15601 gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc ccagcaccag
    15661 gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg
    15721 gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca gaataccgct
    15781 gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg
    15841 atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc
    15901 aagaccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga acatccaact
    15961 gacactgctt tcatgctgct ggatcattgc aatcagggta atcgtcagaa agaccgccat
    16021 tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt taagcccctt
    16081 accacccagc agagaaaaga ccgctgcagc atcggtggag ccgacgatag cgccgattaa
    16141 taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg ttaaaccaga
    16201 ggtgataagt acgcccagcg tcgccagcga cagcgccggt cctaacgcca cacgaaagga
    16261 actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagcgcca ggttacttac
    16321 catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga ctcccgccag
    16381 catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag aaaatgaact
    16441 aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa ttattgttgt
    16501 ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc taccatatta
    16561 gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt ttaagaacag
    16621 gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg gcatgtgcgc
    16681 ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta atgagtggaa
    16741 tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct gcgtcataat
    16801 cattgggcgt gctgccgcta aagctaccga gaataatcgc cttctgacgt ggcaaaattc
    16861 ccgcatgata gagctgcaac agcatacgtt cgacgcggaa agggtgctcg ttaatatctt
    16921 ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccaatc agtgaaatca
    16981 gcatcgcaag attgcctccc cacaacgtgc cttcggtctg acatgtcggg ccttcccctt
    17041 gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc tcggtaaagg
    17101 cgttcagttc atccgcacca aagtttgcta ccagcatggg gccgctaaag gtgatgacat
    17161 tgccttgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgtccg caaatgagta
    17221 acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc agtaaacgac
    17281 tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtagtc agtctggcaa
    17341 gggaattgag atcttccaga cgctccgttt ccgtacccgc aaaacgctca caacgacggg
    17401 caataacctc gacgttattg acttgatgcc ccgcgtcggt aaggcgctgg ataccacgca
    17461 gcgcggcgtg ctgtttaatg ctgtaacccg atggggcaat taagtgaaac agagacatgg
    17521 caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc
    17581 acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat ggtttgcctt
    17641 tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc cgccgtggaa
    17701 cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga aagccggtgc
    17761 agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat cgggtggtaa
    17821 tccgaacgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag cttcaacctc
    17881 cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca gcgagctgaa
    17941 aaatccagag cgtaatattt caatgggggc ggcttacctg aatattctgg aaaccggccc
    18001 gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt catacgctaa
    18061 cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat
    18121 caacgatttg gatgctgacg agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca
    18181 ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc
    18241 actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg ctgtaattgc
    18301 cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt ggtgatggtt
    18361 tcattcttgc catcaatcac tttgcgctcg ctggtggaga ttaactgggc gtcaaagtga
    18421 aaaacacccc attgccccat gttgacttca atttgagcga agcgcatacc ttcttgtaat
    18481 tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa atcatacaag
    18541 cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg cggatggagt
    18601 ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg aggcggtacg
    18661 gtaataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac agtaaactcg
    18721 actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag cacggcgatg
    18781 ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat tgccagtatt
    18841 ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc tttgtgcaaa
    18901 tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc atggtaatga
    18961 ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca tagttaacaa
    19021 aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc cgacaaacgc
    19081 ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt tcttgcgacc
    19141 gattcatcga aacgagggcc tgaacatggg aattattgcc tggattattt ttggcctgat
    19201 agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat ttttcctgac
    19261 ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca tgtttggcat
    19321 tggcggctcc atcagcggtt ttaatttgca cagcttccta gtggcggtgg tgggcgctat
    19381 cctggtactg ggcgtattcc gcctcctgcg aagagaataa gattttcata aggcggatag
    19441 cgatacagat gccgctatcc gctttcacat cagaacgtat actcgacacc tgcccagaaa
    19501 ttgcgacctt cttcaataaa cccttcactg taggcatatt gggtatcaaa caggttattg
    19561 accgacgcat tgacgctgaa gccatgacct aaggtgtaat cggctcgaat gtgggtcacc
    19621 gcaaaaccgg cggctttttg tgaaccgtca ctgttgctgt agctggagga acgcgcctct
    19681 tccgacagcg ttacgcttaa cggctcccat ggtttgagtg tcatccatgc ggtcattgtc
    19741 tgcgttggaa gatcggttat cttgccgatg tctttgcgtt tggcgtcagc gtggatcaga
    19801 gcgtagctca atcctacatc cagtatattg ctgattttcc ccttaatacc ggcatccaga
    19861 ccgctgtaat ccaccgtgcc gctgttctga ttttgaatgg tatcggcatt gatattgtgc
    19921 gagaggatgg catcactcac ccggttatag taaacgctga cctcaaatcc ccagtcgcgc
    19981 gtgaaggcac cattccaggt taaatccacc ccgcgagcgc gttccggctt gagctgcggg
    20041 ttaacaatcg ctatctggtt atacgcaggt ttggatgtgg tatagcgttc tttcagcgtc
    20101 ggaaagcgtg tgcggtcata gtacgaaagc gccagagtgt cttcattggc aaagtggtat
    20161 ttccccatca cctgccagtt aaaagctgac tgattgttgt cgtcataatg ggtgatgctg
    20221 ccatctttct cgtgtttttt cgcttctacg ctatcgcgcc agtcatagct gattcccgcc
    20281 acgacatcga cattatcagc agcagcccat tgatattcac tggcgagcga ccaggtacga
    20341 tcttcatagc gatcgtaagc ggcgtgcggc gcaccttttt cccggtgtac gtcatctttc
    20401 cagttaacgg caaacgacag cagatcgttt tcacgcacat cggctgccag ttgtaatccg
    20461 gcaccgtcgc tgtaatcgga ataatggctg tagctgcctt ttttattttt caaatcagcc
    20521 agcgagttgt acatcatcag cgtattttca aaggtgtcgc gatacagccg acttttcagg
    20581 gtaaaacggt cgtttagttg ggtcgctccc tgataataaa aactttcttt gtcatactct
    20641 ggccactgcc agtagcgtga tttttgacca caatttccgc tgtatggcgg gttatctttt
    20701 tcaccatcct gcttaatgta agtcagcgtg tattcatcgt tttcgcgcgg tgtaaaaccg
    20761 agcttcacaa tgccgcgttt atcatcagcc gatgaattaa tcatcttgcc gtgtttgcct
    20821 gcaatatcat tatttacacc atgcggcagg ccgagaaaat cctgctttag ctggctaccg
    20881 ctgacttgca aatacccaag atcgctgctg gcggcaaatg aagcatgcat atcgtaggca
    20941 ttgtcctgac tacggctcca tccctggcga tatcccagac ttgcttcttc agggtggtga
    21001 tattaatggc tccgcccatc tgattaggcc catgaagcag cgacgaatac cctttggaaa
    21061 cttcgactgc ccccagattg ttggtcagaa cccgcgccag atcgaggttg ccgtcatagg
    21121 gaacataaat gggcacaccg tcgaaataga ccggcacctg ccgactatca aagccacgaa
    21181 cttttacctg ttcttcgttg cggctgcctg acttttgcag caccacgccg gggacgacac
    21241 ttaatgcctg agcgacattc tgtttatcca aggctttcat ggtgggttga tcgagaacgg
    21301 tggtcgtcgt cgatgatacc ggactggacc atacggtcag cgtgtcgctc tcctgagccg
    21361 ctacggcctg ctgggtaaag gcaagtgata atgctgtgca gagataacgt tttttgagtc
    21421 tcatagaata aattccgttt aacaaagaat taaatagagg ctgtgggttg cagactgact
    21481 ttaatgtcag ctggtgccgc gtaataaggc gcggaggtaa taaaaaggcg aatgccgcag
    21541 tcgaggtaat ttttgagtgt cgtcagatta attccgccgg tcagagccag cgtgcagtga
    21601 ggagccagcg acggcgctat ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg
    21661 agctgaagta cgtctggttg tgcgcgtaac gcggcgatag cctctttcgg cgtgtcggct
    21721 tcaacaacaa ttttcttctc gggtgcgtgg cgacgtaact gagcgattgc gcctgaccag
    21781 ccctgattgt catgaagaaa atggcggtgg ttggcaaaca ataatatggt ttccgcacat
    21841 ccggcacgat gaattagtcc tccggcagcc agaattgcct gcgaagccag taagcgggtg
    21901 cccggaattg cttttcgggt gcaggcgata ttgccgtcag gataacgttc acgaagcaat
    21961 gccagcattt gagcgagata atcagaaaca ccgcaactcc actccagcac attctggact
    22021 gccttccatc cctgatgaag tgctgcggca ttaccctgcg cacggattag ccgttgaccc
    22081 gcgttcgctt gtgaaccgtc gctgaccgcg tcatcaatag ttaatcccag cgtagttaac
    22141 atcttacacg cgacagaaac gccgctgacg cagccgccct gacggtgaaa aaacgctata
    22201 tagccaggct ggtgtccaat atttaatgct cgcgtggtca ggtcacctcc ctggatatct
    22261 tccagcagta acgcatcgat ttgtgcctga gaaaggaaaa tcatcacagt gcctcctgcg
    22321 gtacgctatc ccaccagatg agcgcccagt cgcggctggc aggggcgagg gcacgagcat
    22381 cttgttgttg ataccagtga tataaccgct cacgctcatc gtcgttaagc gcgccaagac
    22441 tccagctgac gttctgctca aaacgttccc aggtgctgtt atcctgttgg caattttgtc
    22501 cacggataaa atcaacgtgg gcataaattc ccatctgata caggacgttg agcgcaaaga
    22561 tatagttggg taattcgatc acttcccgac cagccgcccg ctgaatggct ggagagacga
    22621 acgaggtgct gaccagatga gttgtataga cgcgaagccg tgcctgatta ttcagtttgc
    22681 tcattgcatg gcgcatatca gccaccagag tggagcggga agccacggca atatcgcagc
    22741 gtggcaggtc actccagtcc tcttcccagg cgcgctggat ccagtggaca ttgtcagcct
    22801 tcagtgcggc ggcgcggcgg gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat
    22861 aaaccgtagt gagtttgtca gccagcgcca gcgatacggt gcctggtccg cagcccatat
    22921 cgaataaggt ctgagcgcct tgcaggtcaa ttttagcgat cagttgttgc aggtagctgt
    22981 ccgttggcga ggcgcaattt tccgccattt tctccgcccg ttgatcccag tgatcgggcg
    23041 ttttttctgt gcggtgagcc agtttgagtt gctgcagata aaggtctgca aaatcaatat
    23101 catcaatgag catgattgtt ccttagcggc taattgcaga gaggtgatga tgaatttggt
    23161 cggcagaaac ccgatacagg gccgccagtt tgttcgttgt taactgttcg gtcggtaagc
    23221 cttgtgttac tcgtccatct ggctctacct ggataatgct gtcggcaatg gcgttggcat
    23281 ggagcggatg gtgagtcgac attaacattg tcatgccgtt atttttgagc tgcgccagcg
    23341 tgtccagaag ttggatctga tgaccgaaat cgaggctgga ggcgggctca tccagtaaca
    23401 acaagcgcgg ttgttggacg agtgcacggg cgattaaagc cagctgacgt tcaccgccgc
    23461 taagcgtatt ccagcgtcgt gtcgccagat gcaaaatgcc cagcttttcc agttgctccg
    23521 tggctttcag gcgttcttgc ttaccgggaa cggaaaaagc gcctatggtg ggggccaggc
    23581 ccatcaacac catatccagc acactgaaag caaaaatacc gtcgtgcgcc tgcggcaccc
    23641 aggcaatagc ctgtgcgcgc tggcgatggc tgagtgtctg cacagggagg ctatccagta
    23701 ggatctcgcc ttttaataac ggtataacgc caagaatgga gcgcatgagt gtggttttgc
    23761 cgcaaccatt cgcgccaagc agacaacaaa ttgttccttg cgacagggtg aaggacacgt
    23821 cgcgtagcac tggatggcgg gagtaaccca gagacgcatg acgaacggca agcaaagtca
    23881 ctggcgacct ccgcgtaaca acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag
    23941 aatgccaagt gggatttctg ttgttccaat gctgcgcgcc agcgtatcgg taagcaggag
    24001 cagaatagcg ccagtacaca ttgccatagg caacagctgc tgatggttat gaccggtcaa
    24061 caagcgggca atgtgcggaa cgaccagacc gatccaacca attatgcccg ctattgctac
    24121 cgtgctggcg gtgattagtg tggcgcagac aatcaatcca aagcgcagac gcgtcacgtt
    24181 cagtcccagc gaacgggctt cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag
    24241 aaaaaacagg ggcaaggagc ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa
    24301 ggtcacggtg gacagtcccc ccagcaacca gaaggtaatt gacggtaact gggtgtaagg
    24361 atcggccagc gttttaattg gcgatatccc cgctccgcac aacgttccta acgcaatgcc
    24421 taccagcacc agggttaaga tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac
    24481 gcccgcgacc accatcagct cgccgcaaaa tgcgtacagc tggatgtaaa caatgggtaa
    24541 tccccacagg attgccgcgc aggcaccaag tccggcaccg gcggagacgc ctaaaatatc
    24601 cggtgaaact aagggattac gcaacattcc ctgataggtg gttccggctc ctgccagcgc
    24661 tgcgcccagt aacagcgcag caagaatacg cggcacgcgg atttgccaga agacaatttg
    24721 ttcctgcaca gacacatttt cttgacgaag aattagcgcc agcagttgct gcatatcgag
    24781 atgataagcc ccacttacgg cggcaaaagt gatactgata gctgccagca gcaaaataca
    24841 tccgtttacg attctcatta gctcgccacc aatttttgat agtcggcatc actgagtgaa
    24901 caatgccaga acagttgggc gtaatgctgc atgtcacttt taaactggcg attgatggcg
    24961 ggatccagcc aggcgtgaag tctgcgtaat cccagaagac ggttgattcc cggcggggca
    25021 tcgagccagc caaagggcaa gccacttaaa aataagatgc gttgttccgc aacggctttc
    25081 acgccctgcc agagaggatc acgacgaata aaatctgcag taacggcctc ctgaaccaga
    25141 ataatatccg gctgccagcg caggagattt tccatggaaa cctgtgtcag gccgtggcga
    25201 tcggctattt gcgcgacgtt gtgcaaacct aataattccg ccgcctcggt atgcaacgaa
    25261 ccctgtaagc ccgtttccag ccctcgggga ccgcgcgcag cataaaagcg gaggttagcg
    25321 gcgggtgagg tggcgaatgc ctgagcttca ccaacgaagt ggctggcgag attggcttgt
    25381 tctgcggcgc ggtgctcttc gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat
    25441 tgcgccagtt ttccgttaag caataaccag gggatttgtg tctgttcgct aacctgccgt
    25501 gcctgggaga tcaaggtttc atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac
    25561 gccataagcc cttccagcga tagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt
    25621 aactggcgaa tgtgctctgg caatggaatt aatgcctgac gggcaaaatc aaaagaggaa
    25681 aaaccgacca ttttttctgg cgcgacagcc agcaatagca aatcggcggg cgcgcctgcg
    25741 ctgacgatgc gtgtaatgtt ttgggcttcg ggaagagatt gcgaagcaaa acgattaact
    25801 gtttgtgaaa aggacggtag agactgcagg agcagggtag atgcaagtgc ctgcgcaaac
    25861 gtccgtctgg ttatgggcat ggtgaacat
//
LOCUS       DAJTXY010000046        25660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTXY010000046 DAJTXY010000000
VERSION     DAJTXY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.046"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..110)
                     /locus_tag="OO566_002027"
     CDS             complement(<1..110)
                     /locus_tag="OO566_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HCR6605322.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHA"
     gene            complement(180..1169)
                     /gene="araF"
                     /locus_tag="OO566_002028"
     CDS             complement(180..1169)
                     /gene="araF"
                     /locus_tag="OO566_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR6605323.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1966..2469
                     /locus_tag="OO566_002029"
     CDS             1966..2469
                     /locus_tag="OO566_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6605324.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2549..2800)
                     /locus_tag="OO566_002030"
     CDS             complement(2549..2800)
                     /locus_tag="OO566_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6605325.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2915..3001)
                     /gene="azuC"
                     /locus_tag="OO566_002031"
     CDS             complement(2915..3001)
                     /gene="azuC"
                     /locus_tag="OO566_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6605326.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3264..3587
                     /locus_tag="OO566_002032"
     CDS             3264..3587
                     /locus_tag="OO566_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6605327.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLH
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3758..4255
                     /gene="ftnA"
                     /locus_tag="OO566_002033"
     CDS             3758..4255
                     /gene="ftnA"
                     /locus_tag="OO566_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6605328.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4292..4531)
                     /locus_tag="OO566_002034"
     CDS             complement(4292..4531)
                     /locus_tag="OO566_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6605329.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4721..5932
                     /gene="tyrP"
                     /locus_tag="OO566_002035"
     CDS             4721..5932
                     /gene="tyrP"
                     /locus_tag="OO566_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6605330.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSMPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5994..6659)
                     /gene="yecA"
                     /locus_tag="OO566_002036"
     CDS             complement(5994..6659)
                     /gene="yecA"
                     /locus_tag="OO566_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6605331.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6855..6941)
                     /locus_tag="OO566_002037"
     tRNA            complement(6855..6941)
                     /locus_tag="OO566_002037"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6905..6907),aa:Leu,seq:taa)
     gene            complement(6954..7027)
                     /locus_tag="OO566_002038"
     tRNA            complement(6954..7027)
                     /locus_tag="OO566_002038"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6993..6995),aa:Cys,seq:gca)
     gene            complement(7309..7857)
                     /gene="pgsA"
                     /locus_tag="OO566_002039"
     CDS             complement(7309..7857)
                     /gene="pgsA"
                     /locus_tag="OO566_002039"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6605332.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7914..9746)
                     /gene="uvrC"
                     /locus_tag="OO566_002040"
     CDS             complement(7914..9746)
                     /gene="uvrC"
                     /locus_tag="OO566_002040"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6605333.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9743..10399)
                     /gene="uvrY"
                     /locus_tag="OO566_002041"
     CDS             complement(9743..10399)
                     /gene="uvrY"
                     /locus_tag="OO566_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6605334.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10695..10871
                     /locus_tag="OO566_002042"
     CDS             10695..10871
                     /locus_tag="OO566_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605335.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10858..11082
                     /locus_tag="OO566_002043"
     CDS             10858..11082
                     /locus_tag="OO566_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6605336.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11115..11735)
                     /gene="sdiA"
                     /locus_tag="OO566_002044"
                     /pseudo
     CDS             complement(11115..11735)
                     /gene="sdiA"
                     /locus_tag="OO566_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11965..12717)
                     /gene="yecC"
                     /locus_tag="OO566_002045"
     CDS             complement(11965..12717)
                     /gene="yecC"
                     /locus_tag="OO566_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6605337.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12714..13382)
                     /gene="tcyL"
                     /locus_tag="OO566_002046"
     CDS             complement(12714..13382)
                     /gene="tcyL"
                     /locus_tag="OO566_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6605338.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13397..14383)
                     /gene="dcyD"
                     /locus_tag="OO566_002047"
     CDS             complement(13397..14383)
                     /gene="dcyD"
                     /locus_tag="OO566_002047"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6605339.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14488..15288)
                     /gene="tcyJ"
                     /locus_tag="OO566_002048"
     CDS             complement(14488..15288)
                     /gene="tcyJ"
                     /locus_tag="OO566_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6605340.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15376..15927)
                     /gene="fliZ"
                     /locus_tag="OO566_002049"
     CDS             complement(15376..15927)
                     /gene="fliZ"
                     /locus_tag="OO566_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6605341.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15973..16692)
                     /gene="fliA"
                     /locus_tag="OO566_002050"
     CDS             complement(15973..16692)
                     /gene="fliA"
                     /locus_tag="OO566_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6605342.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVCHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16857..18509)
                     /gene="fliC"
                     /locus_tag="OO566_002051"
     CDS             complement(16857..18509)
                     /gene="fliC"
                     /locus_tag="OO566_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6605343.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTVGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18775..20181
                     /gene="fliD"
                     /locus_tag="OO566_002052"
     CDS             18775..20181
                     /gene="fliD"
                     /locus_tag="OO566_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6605344.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20206..20616
                     /gene="fliS"
                     /locus_tag="OO566_002053"
     CDS             20206..20616
                     /gene="fliS"
                     /locus_tag="OO566_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6605345.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20616..20981
                     /gene="fliT"
                     /locus_tag="OO566_002054"
     CDS             20616..20981
                     /gene="fliT"
                     /locus_tag="OO566_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6605346.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21059..22546
                     /gene="amyA"
                     /locus_tag="OO566_002055"
     CDS             21059..22546
                     /gene="amyA"
                     /locus_tag="OO566_002055"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6605347.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDCIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22580..22993)
                     /locus_tag="OO566_002056"
     CDS             complement(22580..22993)
                     /locus_tag="OO566_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6605348.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23180..24385
                     /gene="yedE"
                     /locus_tag="OO566_002057"
     CDS             23180..24385
                     /gene="yedE"
                     /locus_tag="OO566_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6605349.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24382..24615
                     /gene="yedF"
                     /locus_tag="OO566_002058"
     CDS             24382..24615
                     /gene="yedF"
                     /locus_tag="OO566_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6605350.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24722..25392
                     /locus_tag="OO566_002059"
                     /pseudo
     CDS             24722..25392
                     /locus_tag="OO566_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25432..>25660)
                     /locus_tag="OO566_002060"
     CDS             complement(25432..>25660)
                     /locus_tag="OO566_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6605351.1"
                     /translation="AYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNIL
                     AAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         6378 a         6555 c         6100 g         6627 t
ORIGIN      
        1 gcatgaacct gaccggcata gcagtcaaaa ctaatatccg tcagcgcctt aacgccggga
       61 aacgttttac cgatgccgcg aaatgagaga tacggggtag actgttgcat aacgtctccg
      121 tgaatcactg gtcgttcaca ccccctctgc atcatgcaga ggggaatttt tccggcaaat
      181 tacttaccgc ctaaaccttt tttctccagt tcttctttaa agttgtcacg cgtgatcagt
      241 accacgtctg taacttcggt aaattttggc gtttcaacgt cttttgctac ccagttgtaa
      301 agcatttcgc tggatttata gccatgtacg tccgggcttg gcagcaggga accgtagaag
      361 ccggttgcct gtgctttaga cagttcgctt actgcatcca caccgttaat accaataccg
      421 atgatatcgg ctgctttaaa gccctgacct tcggttgcac gtacgccgcc aagtacggtg
      481 ctgtcgttca taccgacgat cagccaatgt ttaacttccg gatgttgaac cagcattgag
      541 ttggcagcgt caaatgcccc cgggatgtcg ttagatttgg taggtacctg ataaatttgt
      601 ttttccggga atcctgcagc tttcagtgcg tccatagaac ccgttgtacg gcggcgggcg
      661 gtatccagtt cgttggcggt aatcgccatc actgcgcttt ctttgacatc ccagccacgt
      721 ttctgcatct ctttatacag ttcctggccc tgacgttcgc caattttagt cgccgccatc
      781 atcaccagag gaacggtatc cattggctta cctttggcgt taacaaactg gtcatccact
      841 gcaatgactt tcatatcgta gccacgcgct ttcgcgacga tggcagagcc gagtttcggg
      901 tccggagtac aaataacgaa accttttgcg ccactggctg ccaggctgtc gatcgcgttc
      961 aatgtttttt cgccatccgg cacggcaatc ttaataacct caaaccctaa atccttcccg
     1021 gctttatcgg caaacttcca ttcggtctgg aaccacggct cttccggttg cttcaccaga
     1081 aaaccgagct tcaggttctc cgccatagcg gattgtgaca taacggctgc cagaccaatg
     1141 gctgccaggg ctttagtaaa tttgtgcatg gttctctcca gctttagtgt cgttttgtgt
     1201 agggcaaaaa cgaatgacat tcgttaaatt aatcggaaaa caaagcatta ccttttatct
     1261 aaaagataag tgaccgtgtt gacatagttt tagcgagaaa ttaattctcc ataggagagc
     1321 aatatcacat cgcagaatta cagtgagaac gtgcataaat ttagcgggaa aagacataag
     1381 gaaaagccaa tttgtcagac aaattgttga atgcacagca gattaatcca taagattagc
     1441 ctggaaatcc ttgttgtctt cggtacccat gcgggatgtc ttctttttaa ccagtcaata
     1501 ggccgcatta cctggcgttg agtttttgaa atggtgtaat aaccacaact caaagatgtg
     1561 gaaaatgcgc gtcattcatt tcgccattaa ttatcactgt gctcattaat taacagaaca
     1621 cgtataatga gagccatctc gcaaaaatga aaaaacgttt tataaaatca tcacttcatc
     1681 atgaattcaa attcatcgat taatatcaac aagatacaaa aagcactatc attaaaattc
     1741 attgcattta cattgatttc accaatgaaa tgtaaaaata tataaacttg atgatttaag
     1801 cattttctta taaccgttca gacgttattc ttatttcaga tcatcgtcag aattgactcc
     1861 acgatcacat ttcggaccgg cagaaaggaa ttattctgca aacagtaatt atggtgtttt
     1921 gatttatctt gcacctctcc acttctggat ataaggatat taggtatggc aaccgctgga
     1981 atgcttctca aactcaactc tcaaatgaac cgcgagtttt acgcatccaa tctctacctt
     2041 cacctgagta actggtgttc tgaacagagt ctgaacggca ccgccacttt ccttcgcgcc
     2101 caggcacaga gtaatgtgac ccaaatgatg cgcatgttta actttatgaa gagtgtcggc
     2161 gctaccccca tcgttaaagc cattgatgtt ccaggtgaaa aactgaactc tctggaagaa
     2221 ctgttccaaa aaacgatgga agaatacgag caacgttcaa gcacgctggc gcagttagcc
     2281 gatgaagcga aagaactgaa tgatgattca accgtcaatt tcctgcgcga tctggaaaaa
     2341 gaacagcagc atgatggcct gttgctgcaa accattcttg atgaagtgcg cagtgcgaaa
     2401 cttgcgggta tgtgccctgt gcagaccgac caacatgttc tgaatgtcgt gtcacaccag
     2461 ctgcattgat catacatcgg cgctaataca ttgcgccgat gaagtttttg agaaaccgct
     2521 gcctcatctg tttgaagcag cggttttttt aatgggattc accctgtggg gtaaacttga
     2581 gttcaatcag cgcgatggct ttttggattg cccgcatggt gaccgggtct gcggcagcgg
     2641 gatgatgagt aaagtcgata ttcttcagct gactggacat tttttcacga acttcaacgg
     2701 gcgcgattac atcgagaaca tccagaattt gtttgataac caactggcaa gctaccacat
     2761 cagaaaccag ttcctgatcg gcattcagcg gctgggacat cgtaaactcc tgatagcatt
     2821 ttgaaagccg ttatagtagc ggcttcacat cttcagcgat agtcacatcc accgtcatca
     2881 ggacacaaaa aaacctgccg gagcaggttt tttgttatcg gaacatattg cctggcggta
     2941 cgtctttgaa cgtcttgcaa tagttattga acatactttt caggattttg cgcagtttca
     3001 tcgcggcact ccgaccattt gttatacagg tgttattgtc tttgcgctta taatatgaca
     3061 accatcacaa aaatcaatct ttatgtgata caaatcacat aaatacccct ttaatgttat
     3121 aaaaatgata atcaaaaaac agccccccta tttctgacac ctacagatgg caagaaatag
     3181 cgcctgccag gcatcttttc cggccattgt cgcagcgctg taacgcgtaa aatagtgctt
     3241 tctcttactc ttctggctgg accatgagac ttctgattct gactctttca ttaataacgc
     3301 tggcggggtg tacggttact cgccaggccc atgtgagcga agttgacgcg gcaacaggta
     3361 ttgtacggtt ggtttatgac caggcttttt tgcatcatgc ccataccgat cgttatgtca
     3421 gtcgcgggat cgccgatcgc gcatgccagc aggaaggcta tacccacgcg atcccctttg
     3481 gtcagccagt aggcaactgt agcctttttg cgggttcctt atgtttgaat acggaattca
     3541 ctttgtcata tcagtgccat cattccgcct tccctgtctt tctctaaata ttttaatcag
     3601 cgaggggatc ttcgctgatt aaagaaatag cggtaatgcg ttttattccc attcgtattt
     3661 ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa ttatagtgac
     3721 gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag aaatgattga
     3781 aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc agcaaatgag
     3841 cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca
     3901 ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg gcaatttacc
     3961 gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg aattattcca
     4021 ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg ctcatgctgc
     4081 aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt ctgagcagca
     4141 tgaagaagag aaactgttca aatcgattat tgataaatta agcctggcag gcaaaagcgg
     4201 cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa actaatgctc
     4261 tccggcggca ggtttctgcc gccgtttcaa atcagtgacg gcaaatctta ctctgatcgg
     4321 tcgaaaaacc ttcttctgaa ggtataaatt tgccttttgc tgccagaaac gctaccagct
     4381 ctcccgctgt catcccttct gccgagcacg tgtgaaaacg tgcctgttca ccaaaacgcg
     4441 ctttaatcgc agcttccaga ctggcatgcg tatattgctc gcctgattca atcatcatat
     4501 ttaacacttc atgaccgtga atagagtcca tcgttcctcc tcaaaaaaag cctagcgtag
     4561 cgattaccgc ttatgaagac tttgcgccag cgcaggactg aatgctttta ttgtacattt
     4621 atatttacac aatatgtaac gtcgatttga cgaagcagcc gttatgcctt aacctgcgcc
     4681 gcagatatca ctcataaaga tcgtcaggac agaagaaagc gtgaaaaaca gaaccctggg
     4741 aagtgttttt atcgtggcgg gaaccacaat tggcgcaggc atgctggcaa tgccgctggc
     4801 tgcggccggt gttggtttta gcgttacgtt aatcttgttg attgggcttt gggcgttgat
     4861 gtgctacacg gcgctattac tgctggaggt gtaccagcat gttccggcag ataccggtct
     4921 gggcacgctg gcaaaacgct atctgggacg ctacggtcaa tggctgacgg gcttcagtat
     4981 gatgttctta atgtatgctc tgactgcagc atacatcagc ggtgccggtg aattgttggc
     5041 ctccagcatc agcgactgga caggtatttc aatgtcggca accgctggcg tgctgttgtt
     5101 cacttttgtt gccggtggcg tggtttgtgt tggaacatcg ctggtcgatt tatttaaccg
     5161 ttttctgttc agcgccaaaa ttatttttct ggtggtgatg ctggtattgc tgctaccgca
     5221 tattcacaaa gtgaatcttt taaccctgcc gttgcaacag gggctggcac tgtctgcaat
     5281 cccggtgatt tttacgtcgt ttggttttca cggtagcatg ccgagtattg tcagctatat
     5341 ggatggcaac attcgtaagc tacgctgggt gtttataatc ggtagtgcca tccccctggt
     5401 ggcatatatt ttctggcagg tggcgacgct tggcagcatt gattcaacaa cctttatggg
     5461 attgctggct aatcatgctg gattaaacgg gctgttacag gcgttacgcg aaatggtgcc
     5521 ctctccgcat gttgagctgg cggtgcattt atttgctgat ttagccctcg ccacgtcatt
     5581 tctcggcgtt gcgttaggct tatttgatta tctggctgat ttgtttcagc gttcaaatac
     5641 cgttggtgga cgattgcaaa ctggtgcaat tacctttctg ccgccgttgg cgtttgcact
     5701 gttttatcca cgaggatttg tgatggcgct gggttacgcc ggtgtggcgc tggcggtact
     5761 ggcattgatt atcccttcac tattgacctg gcaaagcaga aagcacaatc ctcaggcggg
     5821 ttaccgggtc aaaggtggtc gtccggcgct ggtggtggtg tttctctgtg gtattgctgt
     5881 gattggcgtg caatttttga ttgcggcagg gttgttacca gaagtggggt gatcagatag
     5941 cctcaaattc cttattgggt gccagaatta acgctgacac ccaatgtgac ctcttaatgc
     6001 aggcagcact gcttaaattt cttaccactg ccgcacggac aaggatcgtt acgccccggt
     6061 ttctcttctg ctttgatcgg ttgctgaaca gctttttcct gcggatgcgc catccagtat
     6121 gcatgtaaat caagcgccgc cagtcgaatg gcatctacgc tctcttcaaa cgcttctggc
     6181 gacattttct ctacccgctc gaagttttcc tcagtaccgt gcagcgcaat cgcctccagc
     6241 gctggtttta aagaatcggg caacgttgac cagtcagaaa gtgccacgcc ccgcatatag
     6301 ccaaagcacc actcctcaac aatcgtcagc tcgctgccat caacttctcg caagccgaat
     6361 aacggctcaa actgctccgg gaattcgttc agacgctctg cggtatcggc catatgttga
     6421 aaagccaggt tcataaagcg cgtcatctct ttctctgacg cccaacgcgg cacatagtca
     6481 gccccacccc acacggcaac cagccactgt tctggttcaa tctcttgcgg agaactcaac
     6541 accgccgtca ataaaccgtc cagctccgcc acatcaagga tggcgtggtc agtgttgtat
     6601 ttggtcagaa tatcgtccag ccattccaac tcactttcgt ttaacggtcc cgttttcata
     6661 cgcttttcct tgtggatctc aactcgccag cacctatctt acatgccggt ccgtatcaga
     6721 gatacttttt gagtggcttt gccggtgatt aaaaattaag gagggtgtaa cgacaagttg
     6781 caggcacaaa aaaaccaccc gaaggtggtt tcacgacact gcttattact ttgattttat
     6841 tcttatcttt cccatggtac ccggagcggg acttgaaccc gcacagcgcg aacgccgagg
     6901 gattttaaat cccttgtgtc taccgattcc accatccggg ctcgggaaga aagtggaggc
     6961 gcgttccgga gtcgaaccgg actagacgga tttgcaatcc gctacataac cgctttgtta
     7021 acgcgccaaa ttcttcaggc ctttcagcca gacatccgct tgacgccgat gtcttttaaa
     7081 ctggagcggg aaacgagact cgaactcgag accccgacct tggcaaggtc atgctctacc
     7141 aactgagcta ttcccgtatt catcaagcaa tcagttaatc acttgatttt attatcgtct
     7201 ggcaatcagt gccgccgttc gatgcgttgc attctactta cctggcgcga tgagtcaacg
     7261 atatttttca ccacttttga tcgtttgctg aaaattacgc cgaaacgatc actgatcaag
     7321 caaatctgca cgcgcagcgc tcaaatattg caacattgac cacagagtca gtaccgcagc
     7381 cacaaagaaa agtgcaatac cggcgtactc aacccaaatg ttcggacgcc acagcagcca
     7441 ggccaacgcc accatctggg cagtggtttt cactttccca atccaggaga cggccacgct
     7501 actgcgttta cccaactccg ccatccattc gcgtagcgca gaaataataa tttcacgggc
     7561 gatcatcgtt gccgccggta atgtcaccca ccagctgtga taatgctcgg ttaccagcac
     7621 catggcgata gccacgagaa ctttatctgc cacagggtca aggaaagcac caaaccgggt
     7681 actctggttc cagcggcgtg ccagaaaacc atcgaaccag tcagtcaccg ccgcgacgca
     7741 gaaaatgagc gcggcggcaa acggcgacca ggtgacaggc agataaaaga ccaatacaaa
     7801 gaatgggata aggatgacac ggaacagtgt aagcaacgta gggatattaa attgcataat
     7861 gacgggtaac tatctgttgt cagtaagatt acccctatgt tgctacagag acatcaatgt
     7921 ttcaacgacc agaagatctt ttctgccaga ccttgcgaaa tacccggcac ttttgcaatt
     7981 tcctcgacgc tggcgttacg taaaccttgc aaaccgccca tatatttcaa caacatttgc
     8041 cgacgttttg gcccgacgcc ttcaatggtt tccagggaac tggtattttt gaccttcgcc
     8101 cgttttttac ggtgcccgcc aatcgcgtga tcatgtgatt catcgcgaat atgctggata
     8161 acatgcagcg cgggtgaatc tggcggcaaa ctaaatccct caccttccgg ctcaaagaac
     8221 agcgtttcca gtccagcctt acgatctgct cctttggcaa cgccaagtag cagcggatga
     8281 tttttatccc atgagacatc cagttcggcg aagacatttt tcgcctgcgc aagctggcct
     8341 ttgccgccgt cgataaggat cacatccggg atcttactgt cgtcaatggc tttaccataa
     8401 cgccgacgca gcacctgatt catcgccgca taatcatcgc ccggcgtgat gcctgtaatg
     8461 ttatagcgcc gatactccgc acgcagcggg ccgttagcat caaacaccac acaggaagcg
     8521 acggtttgtt cgcccatggt atggctgatg tcaaagcact ccatccgctt cacttccggc
     8581 aatttcaaca cgctggcaag cgcggtcagt cgctggtgaa cggtagattg ctgcgaaagt
     8641 ttgctggtta aggccgtcgc cgcattggtg cgcgcgagtt tcagataacg cgccctatcg
     8701 ccgcgaggtt tggtttgaac attaatcttg cgtcccgcca gttctgaaag ggaatcggcg
     8761 agcagcgttt tatcgctaag attaaaatcg agcaggatct cacccggtaa ggtgcgcatc
     8821 tggctgcctt gtaaatagaa ctgacctacg aaggtttcta ccacctcgct cagttccgta
     8881 ccgccaggca ctttcgggaa atagctgcgg ctgccgagca ctttgccctg acgaatgaac
     8941 aatacgtgga cacaagccat gcccgcatcg aacgccacac caataacgtc aaggtcgtcg
     9001 ccggtattgg aaacaaactg tttttcggtg acgcgtcgca ccgcctgaat ttggtcgcga
     9061 atacatgccg cttcttcaaa ctccagattc tggctggcag tttccatacg gctaatgagt
     9121 tgcgtaagca cctgatcatc tttgccagac aaaaacaggc gcacatactc gacctgctga
     9181 gcgtattctt cttcactcac cagtccttca acgcacggtc ccagacagcg ccctatctgg
     9241 tattgcagac acggacgcga gcgattgcga taaacactat tttcgcactg gcgaatgggg
     9301 aaaatctttt gcagtagcgc cagtgtttca cgtacggcat agccattcgg gaacgggccg
     9361 aaatattcac ctttggcatg ctttgcacca cgatgcatcg ccagacgcgg gtgggtatcg
     9421 ccactcagga agataaaagg ataagattta tcatcgcgta gcaaaacgtt gtaacgcggc
     9481 tgatagagtt tgatgtagtt gtgttccagc aacagcgctt cggtttctgt atgcgtaacc
     9541 gttacatcaa tttgctggat ctgggcgacc agcgcttcgg ttttgcgcga agcgaggttg
     9601 ctgcggaaat agctggaaag ccgttttttc aggtctttcg ctttgccgac atagataacc
     9661 gtaccaccag catcgtacat gcgataaacg cctggctggc tggttacggt ttttaaaaac
     9721 gcttttgcgt caaactgatc actcactgac ttgataatgt ctccgcatta cacagaccat
     9781 ggcgaattgc caggtgagtc agctcaacat cgccatgaat gtttagttta ctgaacatac
     9841 gatagcggta gctgttcacc gttttcggac tgagattgag ctgttctgag atctcattga
     9901 ccttctggcc cttagtgatc atcagcataa tctgcaattc acgttcagac aaactggcaa
     9961 atgggctttc tgttttttct ggttcgatct ggcttaacgc catttgttga gcgatatcag
    10021 aagcaatgta acgctgccct gaatagacag aacgaatcgc actcacgact tcctgcggag
    10081 ccgcgccttt gctgaggtag ccagcagcac ctgcctgcat gactttcgct ggtaaagggt
    10141 tttctgtatg gaccgtaagc atgatgattt taacatcagc tgtggaacgc gcgattttac
    10201 gcgtcgcctc aagaccgcca atgcccggca tactcatgtc cattagcacc acgtcaacgg
    10261 catttgtccg gcaccactta acggcgtctt caccgcacga tgcctctccg acgactttta
    10321 taccctttat atcttccaga atgcgtcgta tccctgcgcg caccagttcg tggtcatcaa
    10381 caagtagaac gttgatcaaa ggaatatctc cagaaatagg gataacgcta ctgatagtta
    10441 gtcattcgta tattagcggt ttttattgca actttgaaac gttaaaaatg gtgggtattc
    10501 gatttttctc tcgtttttgt caattcgatt gtccacagtt atggtcacgc ccgtcaccaa
    10561 cggcttaccg gatcgtttca cagtaagccc tacgatgaaa tgtgacaaaa attgacttta
    10621 ttcagcaaaa atgaaaatca gcccgctgaa tatgtaacat taattaaccc gaacaatacg
    10681 ggcaaaaaac attcatgata aaaatattta ttggtcatta tattaacgta ttttatagca
    10741 ctgccgatat cacgctcaaa aaacaaccac tgctattttt agcaaagctt atggtatact
    10801 ccgccgcctt aacatttttc accgcaaatt ttcattgcaa catgacgagg aaaataaatg
    10861 agtacgcctg atttttctac tgccgagaat aatcaagaac tggcaaatga agtctcctgc
    10921 ctgaaagcga tgctgacgct gatgctgcag gcgatgggac aagctgacgc gggccgcgtg
    10981 atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc tgcagtattt
    11041 tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt aatataaaac cggctgagag
    11101 cgtgcctttc agccggttat tgacaaaatc atcgctatct ctgctgatgt cttcccttcc
    11161 gccgtccatt tcagaatttc tttttcgcgc ttgctgaaat tcatctctgg cgtcatcact
    11221 atttcatcat ttaaacgcat cagagccatc agactttcgc gcaccagtaa ctgcattttt
    11281 aattgcagtt catcactaag aatgggtatt tcgcgcgtgc tgcaacggga aaaggacaaa
    11341 aagcccagcg cccgatttgg cagcattaaa tactgagtga caccgcggcg taaaccatgt
    11401 gcgtgcgcgg cttcccataa tggctgtgct tcgctgaata agtcatcatt ccacattaaa
    11461 tggtcctgac taaagttttc agggttcagc acaggatcaa ttgcgagaaa attttttgcc
    11521 tgataataac taacccacga ctcagggtaa ttggtgtaga aagccacttt aggtcgcgtg
    11581 aatagtaccg ggtggcgaac acataacgaa tagtaatcgt actccagctg ctgagcctga
    11641 agctctattt catggtagac ctcttctgcg gcctccatcc tctgaaaacg caacagcatc
    11701 gtgcgacgcc agctgaaaaa atccgtatcc tgcatagtaa accgcaacgc ccctgagagt
    11761 gagtatcatt tataatgata attcaaacta tcacataaat aagatttata tataatttat
    11821 attattcagg caatgaatta cttttgcaag ccatcgcatt ctcttatgtt attaatgagt
    11881 tatgctgatt tgttaagcag ttttatcacg cttgaaatgg cgtcccgccc cgacaggtga
    11941 atcgtcgggg ctgatttttt cttattattg cagcagaagc ttctcgagga actggcgggt
    12001 gcgaggctgc tggggatcgg caaataacgc ttttgctgcc ccctgctcga ctatccgccc
    12061 ctggtccata aagatcgccc ggtccgcaac atcccgggca aagctcattt cgtgtgtcac
    12121 aatcaccatc gtgcgctttt cctgcgccag ctggcggatg gtgttcagga cttcgcccac
    12181 cagctctgga tccagcgccg acgttggctc gtcaaacaaa atcacctcag gacgcattgc
    12241 cagtgcacgc gcaatcgcaa cacgctgctg ttgaccgcca gacaaacgac gtggatagct
    12301 ggtttcttta ccggccagcc caactttcgc cagcagctcg cgagcgcgcg ccgtggcctc
    12361 ttctttcggt tcgcctttga cgatcaccgg cccttcaata atgttttcca gcaccgtacg
    12421 atgcggaaac aaattaaagt tctggaagac aaacccgacg tgctgacgca actggcgaat
    12481 cagcgatttt tgctgactta atgaacgtgc agtatcaata gtgatatcgc caacggtgat
    12541 cgttcccgct tccggttgtt ccagcagatt tatgctacgt agcaatgtgg ttttgccgga
    12601 accactcgga ccgataattg ccaccacttc gccaggcttt acctcgaggt cgataccgtg
    12661 cagcaccgtc tgaccgtgga attttttcac caggttctta acttcaatgg cactcatttt
    12721 ggttctctct cctggcgatt aagttgattc tcaaaatggt tctgcaacgt cgataacaca
    12781 gtcgccatga tccagtagat gagcgaagcc gccaggtaca tggtgaacac ctccagcgtg
    12841 cgcgaggtaa tcaactgcgc ctgacggaac agctccggca cctggattgt cgcggccagc
    12901 gacgtatctt ttaccagact gataaagctg ttcgacagcg gcggcaacgc cacccgcgcc
    12961 gcctgtggca aaatcgcgcg acgcatggtc tgccacggcg tcataccaat actggcagcg
    13021 gcttcccact gacctttatc aatagaagaa atggcggcgc gcagcgtttc ggcggcatag
    13081 gcggcagtgt tcagcgacag accaatcatt gctgacggaa tgggatctaa ttcgatacca
    13141 aactgcggca ggccgtaata gatcataaac agttgtgcga tgagcggcgt accacgaaaa
    13201 atagagatat aaaaacgcgc cagccagcgc acgggccaga ttggcgacag gcgcatcagc
    13261 gcgagaataa acccgagtag taagccaaaa aacatgccgc cgatactgag ttgcagggta
    13321 tacccagccc ctttcaacag gaacggcaag gaatcaataa ccagttgtat actttcttgc
    13381 atgagcgttc ccggagttaa acgtggggat gataggcgaa cagcgcaggc gcgccgccgg
    13441 tatgaataaa cagaatcggc ccttcatctt tgaagcgttt ctgactgata ccgtcaatca
    13501 gccccgccat cgcttttccg gtatacacag gatcaagcag aataccttcc agacgcgcca
    13561 gcaatttcac tgcttccatg ccttcgtcgt tcggcacgcc gtagccaggt gcaaaatagt
    13621 catcccagag taaaatttcc gctgatgcgg tcagctccag ttctttcgca atcgcctgtt
    13681 gtaggttaac cactttcggc aattgatcgg caacggaacg cgacacggtc acgccaatca
    13741 gttcgctttc aggcatcagg tgttccagcc caacagccag tccggcgtga gttccggcac
    13801 tgcccgatgc gactaccacc gacgaaatat taaccgcccc ttcacactgt tgcgcgattt
    13861 ccagcgcact ctccacataa cctagcgcgc caagagcatt agaaccgcca accggaatga
    13921 catacgggcg aaagccttgt gcttcgactc gcgtcgccag ctcttccagt tgggcattgg
    13981 gatcggtcag tgcgtcgcac atttcaatct gggtattgaa cagatccagc aacaaacgat
    14041 tgccgttggt taaatagttt tctgcggttg tgccaatagg attttccagc agcgccacgc
    14101 agtgcagacc gagtttcgcc gcgactgcgg cagtctggcg cacatggtta gactggatcg
    14161 cgccggcagt aatcagcgta tcggcacctt cgcgcagggc atctgccgcg agaaattcca
    14221 gcttacgtaa tttattgccg cccattgcca tgggggtgac ctcatcccgt ttgatgaaaa
    14281 tttcccgtcc aagataatca gaaaagcgcg gcagatattc gagcggcgtt ggcgcgccga
    14341 taaactccaa ccgtggaaaa cgggttaaat tatgcagtgg cataacagcc tccgatgcgt
    14401 gttgttgtga ttttcttatt atgcacgctg aaaacgcgta aataaaaaag gcgctagtga
    14461 aagcgccctt ttttgtcatt atgctgatta tttggtcaca tcagcaccaa accatttttc
    14521 ggaaagagct tgcagagtgc cgtctttttg catttccgca attgcatcat tcaccgcttt
    14581 cagcaggtct tcatttcctt tacgcagcgc cacgccagac tcctgacggg agaatgcttc
    14641 accggttact gccagcgtat cgttggtttt cttcaccaga tccagcgccg ccagacgatc
    14701 aacgaggatc gcatcgatac gccctacgcg cagatcctga tatttggtcg ggtcatcatc
    14761 ataggtacgc acatcgacac cctgaacatt ctgccgcagc cactcttcat agttggtgcc
    14821 cagaccgaca cccacttttt tgcctttcag atcagcagcc gttttaatgg tgccttcgtt
    14881 accttttttc accagcgcct gaataccgga aatggtgtaa ggggtagaga aatcgtattt
    14941 tttcttgcgc tcgtcagaaa tggtgacctg attaatcacc acatcaatgc gtttagagtc
    15001 cagcgacgcc agcataccgt cccatttggt cggttttagt gacgcctcaa cgccaagatg
    15061 ttttgccagc tgttgagcaa attccacttc aaaaccggtt aatttgccgt catccccctg
    15121 aaaactgaac ggcggataag tgccttccag ccctaccagc agcgtgccgc gctctttgac
    15181 tttattaagc agaccttcat ctgcaaaact tttaacgctc atgcccgcaa ccagcgccac
    15241 gcccatcaca cccatcaatg cctgacgtcc cagatgtgct aatttcatat tcaccccgaa
    15301 tgttgttatg tctgtttgca gtgtagagcc attgtttgta aacacaaaaa caactccgct
    15361 acatcttatt cttatttaat atatatcaga agcaggcagg ctggaggatt tctgcacaag
    15421 tccggtacag gtttggcgct gatagtgttg atacttccgt aatgcgatgc ggtaattgtt
    15481 ggtgctggtg gtgggcagcc acggggcgag gatttcatca aggaaaccgt cctgcagctg
    15541 atcgagggaa atattttgct catgtagatg attacccaaa cggcgcagac gaacgacata
    15601 ttcacgcacc gtacctgggc tcatttcagt ttgctcaaaa agaaatttct taaagccgat
    15661 aatatcgaaa aaatcactct gcgttttgca atgtaaatca ccgcaaaatc ggcacaatgc
    15721 cgcccatgat tgttgttccg tttgccagcc tttttcatca agcagcgtgt ccaggcggga
    15781 aatctcgatt ttattcacta ttttgccgtc gcgaactaag gtaatgcgat cgagtaattt
    15841 acggcaatgc gcgcaatggg tctggctgtg tttaaagtct ttaagatagc ggcttaatgg
    15901 ccgtcttttc aggtgctgca ccatcattaa gaactcctgg tagtcaaagt taaagtgcgg
    15961 catttactga cgttataact tacccagttt agtgcgtaac cgtttaatgg cctggctgtg
    16021 taactgactg acccgcgatt ccccgacctc cagcaccgcg ccaatctctt tgagattcag
    16081 ctcttcctgg taatagaggg ttaataccag tttttcgcgc tccggcaacg tttcgatggc
    16141 ttccatcacc cgctggcgca gattactgtc cagcagttgt tgtagcgggt tttctcgctg
    16201 atgatcatca gtaaccagtt cgatgctatc gccgtgctct tcacgccact catcgtagga
    16261 gaagagctgg ctgttattgg tgtcgagcag catttgacga taatcggcaa tatcgatccc
    16321 taaacgttcc gctacctcag tttccgtggc gttgcggcca agttcctgct ccagttgccc
    16381 tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg cgcggcaccc agtcacggct
    16441 gcgaagttca tccagcatag cgccacggat acgctgcact gcgtaagttg taaatgccgt
    16501 tccttgtagg gcgtcatagc gttcgacagc attaagtaac ccaatgccgc ccgcctgtag
    16561 cagatcgtca agttccacgc tcgcgggcag tcgaacctgc aggcgcaatg cttcgtgaca
    16621 caccagcggg acataacgct gccacagcga gtgtttatcc attacacctt cagcggtata
    16681 gagtgaattc acgataaaca gccctgcgtt atatgagtta tcggcatgat tatccgtttc
    16741 tgcagggttt ttaatcggac gattagtggg tgaaatgagg ggttatttgg gggttacgca
    16801 aaaagccgaa tgtaaaaaac cccgtcggtg gcggggtttg agaggcattc agacgattaa
    16861 ccctgcagca gagacagaac ctgctgcggt acctggttgg cttttgccag cacggagtta
    16921 ccggcctgca ggataatctg cgctttcgac atgttggaca cttcggtcgc atagtcggcg
    16981 tcctgaatac gggactgcgc ttcagacagg ttggtagtgg tgttgttcag gttggtgact
    17041 gcggaatcca aacggttttg caccgcaccc agggaggagc ggaatttgtc gatggagctg
    17101 atggcttcat ccagagcttt caggggatcg gctgtcgtag ctgcttttgt ttcagcatct
    17161 gtcgtcaatt taccatcagc atctttataa actgccttac cgctgccgtt agtcactgaa
    17221 ccatcatcct gtaggtaaaa atctacagta tcagatgtgg tcaacgcctc gttgttatca
    17281 acataaaccg aatcaacacc ttttgttaca gcgccagttg tgccgttgac gctataacct
    17341 acagtagtga aatcaacatt gtttcccgag gttgatacag ctttatcgaa agctgcctga
    17401 tcaacttttg cacctgttac tgaataagta atagtgtccg gcgtagtact attaccggta
    17461 aaggagatag aggtaccgcc aatgtcaatg gttgtcgctt tggtattatt ggctgcaagt
    17521 gtggacagac tagcctcagt caaagaagca ccagagccgt ttttcgttaa gcgcccagtt
    17581 gtatcaatgt agagtttatc tccattgctt gccgtaattt taccatcact gctgatctga
    17641 acatcctgtg cagaaccatc gatagtaata gtgcccttag cggtatcacc aactcccggg
    17701 gtcaaatatt tctgaacatc ggcagctgaa gccgttgtgg tatcaaaaga gtatgactta
    17761 cttgcactgt catacttgta attcgttgca ctagccgccg ttccgaagcc gttattaatg
    17821 gttgcctgaa cagtatcacc atcactaact ccagccagca aatcagacgc tttagcagca
    17881 tcgtaacccg cactcacagt atatttgttg ccgaccactg ttgcattagc agctaccaag
    17941 tcagctttag atgcagcagt attagccaca gccccgccac cattcacatt aaacccattc
    18001 agccccagcg tatcagagtc aattttcttc agatcaatag tgatagtctg gccgtcattc
    18061 gcaccaacct gaattttcat cgaaccgtct tttgccagta cgttcacgcc gttgaactgg
    18121 gtctgaccgg atacgcggtc aatttcgtcg agacgggatt tgatttcgtc ctgaatggag
    18181 tccagatccg aatcagagtt agtcccggta gaagcctgaa ccgtcagctc acggatacgc
    18241 tgtaagttgt tgttgatttc ggacagcgcg ccttcagtgg tctgtgcaac agaaataccg
    18301 tcgttggcgt tacgtgcagc ctgagtcagg cctttaatgt tagaagtaaa acggttagca
    18361 atcgcctgac ccgcggcgtc atccttcgcg ctgttaatac gcaagccaga agacagacgc
    18421 tcgatagaac tcgacagcgc agactggttc ttgttgatat tattttgagt gatcagcgag
    18481 aggctgttgg tattaatgac ttgtgccatg attcgttatc ctatattgca agtcgttgat
    18541 tacgttttgg gtttccaccc gtcggctcaa tcgccgtcaa ccctgttatc gtctgtcgta
    18601 aaacaacctt tagaattttt ttcacaaaca gccatttttt gttagtcgac gaaatactct
    18661 tttctctgcc ccttattccc gctattaaaa aaaacaatta aacgtaaact ttgcgcaatt
    18721 caggccgata accccggtat tcgttttacg tgtcgaaaga taaaaggaaa tcgcatggca
    18781 agtatttcat cgctgggagt cgggtcaggt ctggatttaa gttcaatcct tgatagcctc
    18841 accgccgcgc aaaaagcgac gctaacccct atttcaaatc agcaatcgtc gtttaccgct
    18901 aaacttagcg cctacggtac gctgaaaagc gcgctgacga ctttccagac cgccaatact
    18961 gcattgtcta aagccgatct tttttccgcc accagcacca ccagcagcac caccgcgttc
    19021 agtgccacca ccgcgggtaa cgccatcgcc gggaaataca ccatcagcgt cacccatctg
    19081 gcgcaggcgc aaaccctgac cacgcgcacc accagagatg atacgaaaac ggcgatcgcc
    19141 accagcgaca gcaaactcac cattcaacaa ggcggcgaca aagatccgat taccattgat
    19201 atcagcgcgg ctaactcatc gttaagcggg atccgtgatg ccatcaacaa cgcaaaagcg
    19261 ggcgtaagtg cgagcatcat taacgtgggt aacggtgaat atcgtctgtc agtcacatca
    19321 aatgacaccg gccttgataa tgcgatgaca ctctcggtca gcggtgatga tgcgctacaa
    19381 agttttatgg gctatgacgc cagtgccagc agcaacggta tggaggtctc ggtcgccgcc
    19441 cagaatgcgc agctgacggt caacaacgtc gccatcgaga acagcagcaa cactatcagc
    19501 gacgcgctgg aaaacatcac cctgaacctg aacgatgtca ccacgggcaa ccagacgcta
    19561 accatcactc aggacacctc caaagcgcaa acggcgatta aagactgggt gaatgcctat
    19621 aactcgctaa tagatacctt cagcagcctg accaaataca ccgccgtaga tgcgggagct
    19681 gatagccaga gttctagcaa tggcgcactg ctcggcgact ccacgctgcg gacgattcag
    19741 acgcagttga agtcgatgct gagtaatacc gtcagttctt ccagctataa aacgctggcg
    19801 cagattggta tcacgaccga tcccagcgat ggcaaactgg aactggatgc cgacaaactc
    19861 accgctgcac tgaaaaaaga tgccagcggc gtaggtgcat tgattgttgg cgatggtaaa
    19921 aaaaccggca tcacaaccac catcggcagc aacctgacca gttggctttc gacaacgggc
    19981 attattaaag ccgctaccga tggcgttagt aaaaccctga ataagttaac taaagactac
    20041 aacgccgcca gcgatcgcat tgatgcgcag gtcgcgcgct acaaagaaca atttacccaa
    20101 ctggacgttt taatgacctc gttaaacagc accagcagct acttaacgca gcagttcgaa
    20161 aacaacagta attccaagta atcattattc atcgggagac aggtcatgta cgcggcaaaa
    20221 ggcacccagg cctatgcgca aattggcgtc gaaagcaccg taatgagcgc cagccaacag
    20281 cagttggtca ccatgctatt tgatggagtg ctgagcgcac tggttagagc gcgactgttt
    20341 atgcaggaca acaatcagca aggcaaaggc gtctctttgt caaaagcgat caacatcatt
    20401 gagaacggac tgcgggtgag tcttgatgaa gagagcaaag acgaattaac ccaaaactta
    20461 attgctcttt atagctatat ggtcaggcgt ttgctgcaag ccaatttacg caacgatgtc
    20521 tccgcagtcg aagaggtgga agcattaatg cgtaatattg ccgatgcctg gaaagagtct
    20581 ttactctccc cttcttcgat tcaggaccca gtctgatgaa caatgcaccg catttgtatt
    20641 tcgcctggca acaactcgtc gaaaaaagcc agctcatgtt acgcctggca acggaagaac
    20701 aatgggacga actcatcgct agcgaaatgg cgtatgtgaa tgcggtgcag gagatcgcac
    20761 acctgactga agaggttgcc ccgtccacta cgatgcagga gcagctccgc ccgatgctgc
    20821 acctgattct cgacaacgaa agcaaggtaa agcagttatt acagattcgg atggatgaac
    20881 tggcgaaact ggtcggtcag tcatcggtgc aaaaatcggt gttaagtgcc tatggcgatc
    20941 agggcggctt tgtgctggct ccgcaggata acctcttttg aatcttaatg agccgatggc
    21001 tcgcgaataa tccgattacg gctacgcttc taatattccc cttgaatgga gtcgaagaat
    21061 gcgtaatccc acgctgttac aatgttttca ctggtattac ccggaaggcg gtaagctctg
    21121 gcctgaactg gccgagcgcg cagacggttt taatgatatt ggtatcaata tggtctggtt
    21181 gccgcccgcc tataaaggcg catcgggcgg ctattcggtc ggctacgact cctatgattt
    21241 atttgatcta ggtgagtttg atcagaaagg cagcatccct actaaatatg gtgataaagc
    21301 acaactgctg gccgccattg acgccctgaa acgtaatgac attgcggtgc tgctggacgt
    21361 ggtagtcaac cacaaaatgg gcgcggatga aaaagaagct attcgcgtgc agcgtgtaaa
    21421 tgctgatgac cgtacgcaaa ttgacgaaga aatcattgag tgtgaaggct ggacgcgtta
    21481 caccttcccc gctcgtgccg ggcaatactc gcagtttatc tgggatttca aatgttttag
    21541 cggtatcgac catatcgaac accctgatga agattgcatt tttaaaattg ttaacgacta
    21601 taccggagaa ggctggaacg atcaggttga tgatgaactc ggtaatttcg attatctgat
    21661 gggtgagaat atcgattttc gcaatcatgc cgtgacggaa gagattaaat actgggcgcg
    21721 ctgggtgatg gaacaaacgc aatgcgacgg ctttcgtctt gatgcggtca aacatattcc
    21781 ggcatggttt tataaagagt ggatcgaaca cgtacaggaa gttgcgccaa agccgctgtt
    21841 tattgtggct gaatactggt cgcatgaagt cgataaactg caaacgtata ttgatcaggt
    21901 ggaaggcaaa accatgctgt ttgatgcgcc gctgcagatg aaattccatg aagcatcgcg
    21961 catgggtcgc gactacgaca tgacgcagat cttcactggg acgctggtgg aagccgatcc
    22021 ttttcacgcc gtaaccctcg ttgccaacca cgacacccaa ccgttgcaag ccctcgaagc
    22081 accggtcgaa ccgtggttta aaccgctggc gtatgcctta attttgttgc gggaaaatgg
    22141 cgttccttcg gtgttctatc cggacctcta cggtgcacat tacgaagatg tcggtggtga
    22201 cgggcaaacc tatccgatag atatgccaat aatcgaacag cttgatgagt taattctcgc
    22261 ccgtcagcgt ttcgcccacg gtgtacagac attatttttc gaccatccga actgcattgc
    22321 ctttagccgc agtggcaccg acgaatatcc cggctgcgtg gtggtcatgt cgaacggaga
    22381 tgatggcgaa aaaaccattc atctgggaga gaattacggc aataaaacct ggcgtgattt
    22441 tctggggaac cggcaagaga gcgtagtgac agacgaaaac ggcgaagcga ccttcttttg
    22501 caacggcggc agcgtcagcg tgtgggttat cgaagaggtg atttaaatcc atccccggcg
    22561 gcaagccggg gagatttcat tacggcagtg gcgtcggtaa cggcgcttta tccagcgcgg
    22621 cggcacactc tgccgtcggg cgatccacac gctccatcgt cataccatcg tactcaatgg
    22681 tgttaccgtc acgctcaacc tcgtacagct cacgttttac ggtaacgtta gtgagatcat
    22741 cagacatcaa tgtcaactta cccggcacgg caatcccccg ctgccactga cggcaatcca
    22801 aggtatcgcc ctctttagtg acaatcaggc tgcctatcgc ttccggactg accagcgcac
    22861 gttgcggccc tttggtctgc cagtagcccg ccagccagtc cggcgcgggg gttttcacga
    22921 cattgttata gttttcgact tcggcacagc ccgccagcaa catgagcgcg cctgcaattg
    22981 cgagtttttt catcattcgt cctgcatgag aagaaaagag gattgtggca ttaaagctct
    23041 gatgtcgcca gcctttccag gttcagagag aaatttgatc taactattat ttttgtgaat
    23101 tattaccccg aaaataaatt tgcatggtgt atgattcgcg gctcttgttt caaaccttct
    23161 gagttcagag gctacattca tgtcatggca gcaattcaaa cacgcctggt tgattaaatt
    23221 ctgggcaccc atccctgcgg tcatcgcggc gggtattctc tccacttact attttggcat
    23281 tactggcacc ttttgggctg tcacgggtga atttacccgt tggggcggtc agctcctgca
    23341 gctgtttggc gtccatgctg aagagtgggg ttattttaaa attatccatc tggaaggatc
    23401 gccattaacc cgcatcgacg gcatgatgat cctcggtatg tttggcggct gctttgctgc
    23461 tgcgctgtgg gccaacaatg tcaaactacg catgccacgc agccgtatcc gcattatgca
    23521 ggccatcatt ggcggcatta tcgccggttt tggcgcgcgt ctggcaatgg gctgtaacct
    23581 ggcggcgttc tttaccggca ttcctcagtt ctcgctgcat gcctggttct ttgccatcgc
    23641 cactgccatt ggttcatggt ttggcgcgcg ctttaccctg ctgcccatct tccgtattcc
    23701 cgtgaaaatg cagaaagttt ctgccgcctc accgctgacg caaaaaccgg atcaggcgcg
    23761 gcgtcgtttt cgtctcggga tgctggtctt tttcggcatg ctgggctggg cgctgctcac
    23821 ggcgatgaac caaccaaaac tggggctggc aatgctgttt ggcgtcggct ttggtttact
    23881 gattgaacgt gcgcaaatct gctttacttc ggcgttccgc gatatgtgga tcaccggacg
    23941 tacccatatg gcgaaagcaa tcattatcgg tatggcggtg agtgccatcg ggatcttcag
    24001 ttacgtacag ttaggcgttg aacctaaaat catgtgggcg ggaccaaatg cggtaattgg
    24061 tggtttactt tttggttttg gcatcgtgct ggcaggcggc tgcgaaaccg gctggatgta
    24121 ccgcgcggta gaaggccagg tgcactactg gtgggtcggt ctgggcaacg tgatcggctc
    24181 aacgattctg gcgtattact gggatgattt cgctccggcg ctggccaccg actgggacaa
    24241 aatcaacctg ctgaaaactt ttggtccgat gggaggcctg ctggtgacat atttgctgct
    24301 gtttgctgcg ctgatgttga ttatcggctg ggaaaaacgc ttcttccgcc gtgcggcacc
    24361 gcagactgct aaggagatcg catgaaaaat atcgttcctg attaccgcct tgatatggtg
    24421 ggtgaaccct gcccttatcc ggcagtcgca acccttgagg cgatgccgca attaaaaaaa
    24481 ggtgagatcc tagaagtggt gagcgactgt ccgcagtcga tcaataatat tccactggat
    24541 gcgcgtaatc acggctacac tgtgctggat attcagcaag acgggccgac cattcgttat
    24601 ttaattcaga agtaatccct cattcccgct gtggcccaca gcgggaacat cgttacctct
    24661 gacacataga ttttacccgc cccctctcct attcttttca gtatgatctc aggaggttgc
    24721 tatgtgtgga cgctgtgccc aatcccaaac gcgtgaggat taccttgcgc ttctcgcgga
    24781 agatattgaa cgcgatattc cctacgatcc cgaacccatt ggcagataca atgtcgcgcc
    24841 gggaaccaaa gttcttctgc tcagtgaacg tgatgaacac cttcatctgg atccggtttt
    24901 ctggggatat gctcccggat ggtgggataa accgccgctg attaacgccc gcgtagaaac
    24961 tgcctcaacc agtcgcatgt ttaaaccgct ctggcaacat ggtcgggcaa tctgttttgc
    25021 cgatggctgg tttgagtgga aaaaagaagg cgacaaaaaa agccttattt tatctatcgc
    25081 gctgacggac aacctgtttt tatggccgcg ataggtagca taccgtttga gcgtggtgac
    25141 gaagccgaag gatttttgat cgtcactgcg gcggcagatc aaggtctggt agatattcat
    25201 gaccgccgcc cgctggttct gtcgccagaa gctgcacggg aatggatgcg gcaagacatt
    25261 ggtggtaaag aagcctcaga aatcgctacc agaagctgtg tgccagcaaa ccagttcacc
    25321 tggcaccctg tgtcgcgcac ggtgggtaat gtcaaaaacc agggcgcgga gttaattcaa
    25381 cctgtttgtt gacatcctcc acgccctgaa tgacgaggcc ccctgctacg ttcaggctgt
    25441 cgcctgaatc atttcggtgg gttcctgctt caacgagcgg cctgactgca ccatctctcc
    25501 acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gcgccattta catcggcgtt
    25561 cgctgtatat ccacatacct ggcatctgaa tttactttgt gacaggcgat tttctttcgc
    25621 tgtatgacca cagcacgcgc aacgctggct tgtgtacgct
//
LOCUS       DAJTXY010000047        25362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTXY010000047 DAJTXY010000000
VERSION     DAJTXY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.047"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..519
                     /locus_tag="OO566_002061"
     CDS             1..519
                     /locus_tag="OO566_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6605352.1"
                     /translation="MVAAITCISQPKKTTLRDKAMVNYAFDYLRSPGSLPLTTAATEL
                     SAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGL
                     GVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYK
                     NAERLLAGLPAH"
     gene            complement(529..1956)
                     /locus_tag="OO566_002062"
     CDS             complement(529..1956)
                     /locus_tag="OO566_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6605353.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1953..2660)
                     /locus_tag="OO566_002063"
     CDS             complement(1953..2660)
                     /locus_tag="OO566_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6605354.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2818..3801
                     /locus_tag="OO566_002064"
     CDS             2818..3801
                     /locus_tag="OO566_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6605355.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3871..4353)
                     /locus_tag="OO566_002065"
     CDS             complement(3871..4353)
                     /locus_tag="OO566_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6605356.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4588..7170
                     /gene="leuS"
                     /locus_tag="OO566_002066"
     CDS             4588..7170
                     /gene="leuS"
                     /locus_tag="OO566_002066"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6605357.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7185..7766
                     /gene="lptE"
                     /locus_tag="OO566_002067"
     CDS             7185..7766
                     /gene="lptE"
                     /locus_tag="OO566_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6605358.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7766..8797
                     /gene="holA"
                     /locus_tag="OO566_002068"
     CDS             7766..8797
                     /gene="holA"
                     /locus_tag="OO566_002068"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6605359.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRTLFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8799..9440
                     /gene="nadD"
                     /locus_tag="OO566_002069"
     CDS             8799..9440
                     /gene="nadD"
                     /locus_tag="OO566_002069"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6605360.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9464..10075
                     /locus_tag="OO566_002070"
     CDS             9464..10075
                     /locus_tag="OO566_002070"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6605361.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10335..10652
                     /gene="rsfS"
                     /locus_tag="OO566_002071"
                     /gene_synonym="rsfA"
     CDS             10335..10652
                     /gene="rsfS"
                     /locus_tag="OO566_002071"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6605362.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10656..11123
                     /gene="rlmH"
                     /locus_tag="OO566_002072"
     CDS             10656..11123
                     /gene="rlmH"
                     /locus_tag="OO566_002072"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6605363.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11154..13055
                     /gene="mrdA"
                     /locus_tag="OO566_002073"
     CDS             11154..13055
                     /gene="mrdA"
                     /locus_tag="OO566_002073"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6605364.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13058..14170
                     /gene="mrdB"
                     /locus_tag="OO566_002074"
                     /gene_synonym="rodA"
     CDS             13058..14170
                     /gene="mrdB"
                     /locus_tag="OO566_002074"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6605365.1"
                     /translation="MTANPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14181..15269
                     /gene="rlpA"
                     /locus_tag="OO566_002075"
     CDS             14181..15269
                     /gene="rlpA"
                     /locus_tag="OO566_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6605366.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15408..16619
                     /gene="dacA"
                     /locus_tag="OO566_002076"
     CDS             15408..16619
                     /gene="dacA"
                     /locus_tag="OO566_002076"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6605367.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16730..16993
                     /locus_tag="OO566_002077"
     CDS             16730..16993
                     /locus_tag="OO566_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6605368.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVIEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17094..17735
                     /gene="lipB"
                     /locus_tag="OO566_002078"
     CDS             17094..17735
                     /gene="lipB"
                     /locus_tag="OO566_002078"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6605369.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17994..18947
                     /locus_tag="OO566_002079"
     CDS             17994..18947
                     /locus_tag="OO566_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6605370.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19156..20121
                     /gene="lipA"
                     /locus_tag="OO566_002080"
     CDS             19156..20121
                     /gene="lipA"
                     /locus_tag="OO566_002080"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6605371.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20222..20425)
                     /gene="tatE"
                     /locus_tag="OO566_002081"
     CDS             complement(20222..20425)
                     /gene="tatE"
                     /locus_tag="OO566_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6605372.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20554..21342)
                     /locus_tag="OO566_002082"
     CDS             complement(20554..21342)
                     /locus_tag="OO566_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6605373.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21435..21818
                     /gene="crcB"
                     /locus_tag="OO566_002083"
     CDS             21435..21818
                     /gene="crcB"
                     /locus_tag="OO566_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6605374.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21872..22081)
                     /gene="cspE"
                     /locus_tag="OO566_002084"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21872..22081)
                     /gene="cspE"
                     /locus_tag="OO566_002084"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6605375.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22256..22816)
                     /gene="pagP"
                     /locus_tag="OO566_002085"
     CDS             complement(22256..22816)
                     /gene="pagP"
                     /locus_tag="OO566_002085"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6605376.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23405..24790
                     /gene="dcuC"
                     /locus_tag="OO566_002086"
     CDS             23405..24790
                     /gene="dcuC"
                     /locus_tag="OO566_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6605377.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24831..25172)
                     /locus_tag="OO566_002087"
                     /pseudo
     CDS             complement(24831..25172)
                     /locus_tag="OO566_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         6484 a         6300 c         6239 g         6339 t
ORIGIN      
        1 atagttgcag caattacctg tattagccaa cccaagaaaa cgacgttgcg tgataaagcc
       61 atggtgaatt atgcctttga ttatttaaga tcaccaggca gtctaccgtt aaccacggca
      121 gccacggagc tttccgcgat tcatggtcac tcaacatcgc aatatcgcct tggcgaattt
      181 tatcttcatg gtagcgacgg taaaccactg gattatacac aggcgagata ctggtatgag
      241 caatcagcag aacaggaaaa tccgcgcgca caaagcaaac tggggtggat ctacctgaaa
      301 ggcctgggag tcaaacccga tacccgtaaa gcaattctct ggtataagga agcagctgaa
      361 caagggtatg ctcatgctca atatactttg ggtttgatct acagaaatgg cactggtatt
      421 aatgttaacc attatgaatc tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa
      481 aatgcggaaa gattacttgc cggacttccc gcacattaac gtcgactgtc acccgaagag
      541 atggagtatt ttccctatca aaccggcaaa gataaaaatg tagaaaataa ttttgatgac
      601 gcccagacag cccagcccgc catcgtcaac gggcattggc tgcggttgcg gctccggctc
      661 gacggtttcc tgaccggtaa aattcgccgc ctgaattaat aatcgacgaa taattaatgc
      721 ttccagtgga caacgatctt ctgcatcaag gcggctcaca atcggcatta atgattcttc
      781 tgtcggttgg ctggcctgca ctaattgttt ggcaactaaa tataatggcc ataagggatg
      841 ttctgtatca cgcgtgagtg agatccgcgg cttacgtcct ttgtgcaaaa ccatctcgac
      901 cagtgaacgc cccagcgcag agagtttttc atcgctgagt atttcgtata aatgaaaacg
      961 cgtacgggcg ctgtcgccgg ggacataaag tagcggtttt ccctgatcgt taaccagatc
     1021 acgcgcttca atcatcatca ccactaatgg gtaataatcg acccgatgct gacggcacca
     1081 ttgcaacatt aaagcatccg cctgggtgga gggatactcg cgccagtagt aacaaagttc
     1141 tgccagcagg ctttcgcctt ctccacaata gacgcgttgt gcgtattcat aataacgcgc
     1201 atcttcatta tcaggttcag cttcccgcca cgaccgggcg taatccagga gttcggggat
     1261 tcccaggcgg cttgcgctat accagcggag taaatttctg tgcagttttg catcatcggg
     1321 gataatcact gcgcgatgtt gcgctaaata caatgtcagc cattctggat gcgatgacca
     1381 aatccgagcg aacatttggt aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg
     1441 cagaatggtc tggaagttaa aaacattccc cttcgcaatg gcatataaaa aggattccag
     1501 ttcttcttcg tctatttccc cgacgttttc ctcctgcaac caccgcaatc tttctgctaa
     1561 taacaccacg cactcgaagg ataaatgggc agtgttcatg gcgattgtgc acagcgacca
     1621 gcgtaattca tcaatctctt ccatcgagca ataatttaat tgctgaatga atcgctgcca
     1681 ggcggagggc agaaaacgtt cgctatcaga ggcaagtaac gcgcgaaagg cgagcagaat
     1741 ttcatgttct gctacctcat attcttctgg ttgccaaaca gatttagcag gcgactgcgc
     1801 aatccgtagc gcctcctcat acgcctgacg aagttgttta aaaccctgcg gatcggtttc
     1861 tggatgaaag gacggtaaca gcgccagata agcgcggcgg ataatatcga cgtcagtcgt
     1921 ttcctctata tcgaggatct tccagcaatt tttcataagt cgctcatatc cttaagcgtt
     1981 tccgggcatg tggggtagtc agccagtggt tgccaggaca aggtagggta atacgcatca
     2041 tcactggcaa ataattgata aaacatatca ttgccggaat cgtcgtattc tttacgcgct
     2101 aagaattcgc gtaataaagg cagatgatca tcttcgggag actgccaata ttgccgacca
     2161 aaggtataac cggcaaagta ttgccgccag ttactataat aacgtagagc ccgtagatga
     2221 atacgcgaca ggatccatag actttcttct tcactcaacc agttattaag tacgcccatt
     2281 cgacttaaaa aacccatccg gacataatcc catgctttaa ttcctccctc accacagcat
     2341 tcggctgtgc tggcgacaaa ttgcgcataa gcctgacctt gctcatttaa ttcagcaaga
     2401 tagtcacgcc attcacatgg agaataacga aaccagcggt gataaaaccc tgccaaccga
     2461 gcggcatgac cgttctcgat cattcggtga attgtgcgca gcaggtcatc acggtcatca
     2521 attccccatg aggaagaaag gtcaaaaaag catgtaccta aatacatttt gggggatgaa
     2581 tgcgaaggta aacagcattc attgcgtatt tccatcggag tggaaagtgc aaacaatatt
     2641 ttttgcgacc ccatgtccat ataatttatc cttaaacatt catttaatga atgcaaacta
     2701 catttcgaga ctcagttatt tatacttttc tgacgcaaca taatacgcat aaaataagga
     2761 ttacgcaaaa atggaacaca taaaaatata atatatttta tctacaacca taaggaaatg
     2821 ataataattt ttacgtcaag ttgctgcgat aatttatcaa tagatgagat tatcgaacgt
     2881 gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc
     2941 gcaattgact ctccggacga cgaaaaggcc ttttactggc tgaagctggc cgctgagcaa
     3001 ggtcattgtg aagcacagta ttccttaggg caaaagtata ccgaggataa aagccgtcat
     3061 aaagataatg agcaagccat cttctggctg aaaaaagctg ccctacaagg ccatactttc
     3121 gcttccaacg cccttggctg gatactggat cgtggagaag accccaacta taaagaagcg
     3181 gttacctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttgga
     3241 tggatgtaca gaaatggcaa cggagtcgca caagactata cgctggcatt tttctggtac
     3301 aaacaagctg cattacaagg ccatagttac gcacaagaca atctggccga tctttataaa
     3361 gacggagaag gcgttgctca aaacaagaca ctcgccgcat tctggtattt gaaaagcgca
     3421 cagcagggta atcggcacgc ccagtttcaa attgcatggg attataacgc tggcgaaggg
     3481 gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc tcaggaaagc
     3541 gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat
     3601 tatcaggctg cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg
     3661 tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct ccgacaggct
     3721 ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt
     3781 gagactgaag ctttactgta gaaataaaaa caagggccgg ggaatttcat tctgcccata
     3841 ttctctttat tgctatgcga aatcagacta ttacggctca aacgggcgtc tctggaactg
     3901 gtcataacca catttcgggc atagcgtcag cacttccggt gtgtagatcg ggagatggaa
     3961 gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt gataaacccc
     4021 atgatgattg aggtcctgga aaacttcgcg ccattcaagc tgcgttttat cggtgatgtc
     4081 tgccagctcc tgccacaagc tttctttaat cacccgcata aagacgctgt cagattcttc
     4141 tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctga cagctcgcgt
     4201 cagctcatcg atctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg
     4261 ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgttca gtgacgcgac
     4321 cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga
     4381 ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat
     4441 taatgtacga ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg
     4501 cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tcaacgctac atttgtagcc
     4561 gtattgaaaa caggaccact ggctgccatg caagagcaat accgcccgga agagatagaa
     4621 tccaaagtac agcttcattg ggatgagaag cgcacatttg aagtaaccga agacgagagc
     4681 aaagagaagt attactgcct gtctatgctt ccctatcctt ctggtcgact acacatgggc
     4741 cacgtacgta actacaccat cggtgacgtg atcgcccgtt accagcgcat gctgggcaaa
     4801 aacgttctgc agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg cgcggcggtg
     4861 aaaaacaaca ccgctccggc accgtggacg tacgacaaca tcgcgtatat gaaaaaccag
     4921 ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc tggcaacctg tacgccggaa
     4981 tactaccgtt gggaacagaa attcttcacc gagctgtata aaaaaggcct ggtatataaa
     5041 aagacttctg cggtcaactg gtgcccgaac gaccagaccg tactggcgaa cgaacaagtt
     5101 atcgacggct gctgctggcg ctgcgatacc aaagttgaac gtaaagagat cccgcagtgg
     5161 tttatcaaaa tcactgctta cgctgacgag ctgctcaacg atctggataa actggatcac
     5221 tggccagaca ccgttaaaac catgcagcgt aactggatcg gtcgttccga aggcgtggag
     5281 atcaccttca acgttaacga ctatgacaac acgctgaccg tttacactac ccgcccggac
     5341 acctttatgg gttgtaccta cctggcggta gctgcgggtc atccgctggc gcagaaagcg
     5401 gcggaaaata atcctgaact ggcggccttt attgacgaat gccgtaacac caaagttgcc
     5461 gaagctgaaa tggcgacgct ggagaaaaaa ggcgtcgata ctggctttaa agctgttcac
     5521 ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat ggagtacggc
     5581 acgggcgcag ttatggcggt tccgggtcac gaccagcgcg actacgagtt tgcctctaaa
     5641 tacggcctga acatcaaacc ggttatcctg gcggctgacg gctctgagcc agatctttct
     5701 cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg gcgagttcaa cggtcttgac
     5761 catgaagcgg ccttcaacgc catcgccgat aaactgactg cgatgggcgt tggcgagcgt
     5821 aaagtgaact accgcctgcg cgactggggt gtttctcgtc agcgttactg gggcgcgccg
     5881 attccgatgg tgacgctgga agacggtacc gtaatgccaa ccccggacga ccagctgccg
     5941 gtgatcctgc cggaagatgt ggtgatggac ggcattacca gcccgattaa agcagatccg
     6001 gagtgggcaa aaactaccgt taacggtatg ccagcgctgc gtgaaaccga cactttcgac
     6061 acctttatgg agtcctcctg gtactatgcg cgctacactt gcccagagta caaagaaggt
     6121 atgctggatt ccgaagcggc taactactgg ctgccggtgg atatctacat tggtggtatc
     6181 gaacacgcca ttatgcacct gctctacttc cgcttcttcc acaaactgat gcgtgatgca
     6241 ggcatggtga actctgacga accagcgaaa cagttgctgt gtcagggtat ggtgctggca
     6301 gatgccttct actatgttgg cgaaaacggc gaacgtaact gggtttcccc ggttgatgct
     6361 atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc aggccatgaa
     6421 ctggtttata ccggcatgag caaaatgtcc aagtcgaaga acaacggtat cgacccgcag
     6481 gtgatggttg aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt tgcttctccg
     6541 gctgatatga ctctcgaatg gcaggaatcc ggtgtggaag gggctaaccg cttcctgaaa
     6601 cgtgtctgga aactggtgta cgagcacaca gcaaaaggtg atgttgcggc actgaacgtt
     6661 gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac tatcgctaaa
     6721 gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg caattgcggc gattatggag
     6781 ctgatgaaca aactggcgaa agcaccgacc gatggcgagc aggatcgcgc tctgatgcag
     6841 gaagcactgc tggccgttgt ccgtatgctc aacccgttca ccccgcacat ctgcttcacg
     6901 ctgtggcagg aactcaaagg cgaaggcgat atcgacaacg cgccgtggcc ggttgctgac
     6961 gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg taaagtccgt
     7021 gccaaaatca ccgttccggt ggacgcaacg gaagaacagg ttcgcgaacg tgctggccag
     7081 gaacatctgg tagcaaaata tcttgatggc gttactgtac gtaaagtgat ttacgtacca
     7141 ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg aagcgtgcga tatctggcaa
     7201 cattgttgtt atctctggcg gtgttaatca ccgccgggtg tggctggcat ctgcgtgaca
     7261 ccacgcaggt tccttccact atgaaggtca tgatcctaga ctcaggcgat ccgaacgggc
     7321 cattaagccg tgcggtgcgt aaccagttac gtctgaatgg tgtcgagttg cttgataaag
     7381 aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa agtgagcatc gcgaaagata
     7441 ccgcatcggt attccgtaac ggtcaaacag cagagtatca gatgatcatg acggtgaatg
     7501 cgaccgtgtt gatccccggc cgtgatatct acccgattag cgccaaagtc ttccgttcat
     7561 tcttcgataa cccgcaaatg gcgttagcga aagataacga acaagacatg atcgtaaaag
     7621 agatgtacga ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc cgtgctgcgg
     7681 atattcgttc cgacgaagaa cagacgtcga ccacaacgga tactccggca acgcctgcac
     7741 gcgtctccac catgctgggt aactgatgat tcggttgtac ccggaacaac tccgcgcgca
     7801 gctcaatgaa gggctgcgcg cggcgtatct tttacttggt aacgatcctc tgttattgca
     7861 ggaaagccag gacgctgttc gccaggtagc tgcggcacaa ggattcgaag aacaccacac
     7921 tttttccatt gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc aggctatgag
     7981 tctgtttgcc agtcgacaaa cgctattgct gttgttacca gaaaacggac cgaatgcggc
     8041 gatcaatgaa caacttctca cactcaccgg acttctgcat gacgacctgc tgttgatcgt
     8101 ccgcggtaat aaattaagca aagcgcaaga aaatgccgcc tggtttactg ctcttgcgaa
     8161 tcgcagcgtg caggtgacct gtcagacacc ggagcaggct cagcttcccc gctgggttgc
     8221 tgtgcgcgca aaacagctca acttagaact ggatgacgcg gcgaatcagg tgctctgcta
     8281 ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg gagcgtttat cgctgctctg
     8341 gccagacggc aaactgacat taccgcgcgt tgaacaggcg gtgaatgatg ccgcgcattt
     8401 cacccctttt cattgggttg atgctttgtt gatgggaaaa agtaagcgcg cgttgcatat
     8461 tcttcagcaa ctgcgtctgg aaggcagcga gccggttatt ttgttgcgca cattacaacg
     8521 tgaactgttg ttactggtta acctgaaacg ccagtctgcc catacgccac tgcgtacgtt
     8581 gtttgataag catcgggtat ggcagaaccg ccggggcatg atgggcgagg cgttaaatcg
     8641 cttaagtcag ccgcagttac gtcaggccgt gcaactcctg acacgaacgg aactcaccct
     8701 caaacaagat tacggtcagt cagtgtgggc agagctggaa gggttatctc ttctgttgtg
     8761 ccataaaccc ctggcggacg tatttatcga cggttgatat gaaatcttta caggctctgt
     8821 ttggcggcac ctttgatccg gtgcactatg gtcatctaaa acccgtggaa acgctggcga
     8881 atttgattgg tctgacgcgg gtcacaatca tccctaataa tgttcctccg catcgtcccc
     8941 agccggaagc gaacagcgtg cagcgtaaac acatgcttga actggcgatt gccgacaagc
     9001 cattatttac tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac actgcacaaa
     9061 cactgaaaga gtggcggcag gaacaaggac cggacgtgcc gctggcgttt attattggtc
     9121 aggattcatt gctgaccttt ccgacctggt acgaatacga aacgatactc gacaatgcac
     9181 atttgatcgt ctgtcggcgt ccaggttacc cacttgaaat ggcgcaaccg caataccagc
     9241 aatggctgga agatcatttg acacataacc cggaagatct tcaccttcag cctgccggta
     9301 aaatttatct ggctgaaacg ccgtggttta acatctcggc gaccatcatc cgcgaacgtt
     9361 tgcaaaacgg tgaatcgtgt gaggatttat tgccggaacc ggtattgact tacattaacc
     9421 aacaaggctt gtatcgctga tacctgtcgt tacggagggc atgatgcgac tgtggttaat
     9481 tcgtcatggc gaaacacaag cgaatgtcga tggtctgtac agcggtcatg cgcccacacc
     9541 cctgaccgca cgcggtattg agcaagcgca aaatctgcat acactgctag atgatgtttc
     9601 ctttgacctg gttttatgca gtgaactgga acgggcacag cataccgcgc gactggttct
     9661 cagtgaccgc cagctccccg ttcatatcat ccctgaactc aacgaaatgt tttttggcga
     9721 ctgggagatg cgacatcatc gcgacctcat gcaagaagat gccgaaaact atagcgcgtg
     9781 gtgcaatgac tggcagcatg ctatccccac gaacggtgaa ggatttcagg cattttcgca
     9841 acgtgtggaa cgctttatcg caaggcttag tgaatatcag cactatcaga atattttaat
     9901 cgtcagccat cagggcgtac tgagtctgtt aattgctcgt ttaattggca tgcctgccga
     9961 gtctatgtgg cattttcgcg ttgaccaggg gtgttggagc gccattgata tcaaccaaaa
    10021 attcgcgacg ctacgcgtcc tcaatagccg tgccatcggg gtcgagaatg catgactttt
    10081 ctgttttttt acgggtaagc cgcaacgacc attgacagcc ccgggcaggc tgatattctc
    10141 cgcagccaga ctttttccgc cagacacgac tttgtagaaa ttgttttaca aaaatggcga
    10201 tgcaatctgc ggcgcggggt gggatgatag cccactttcg aaagccgatt cggcgacaat
    10261 tgtcccgaaa tcgcctctgg ttcaggtata ctgacagacc atttttatct atttgattca
    10321 cccaggggga aaacttgcag ggtaaagcac tccaggattt tgttatcgac aaaattgatg
    10381 acctcaaagg tcaggacatc atcgccttag acgttcaagg caaatccagc atcaccgact
    10441 gcatgatcat ctgtacgggt acgtccagcc gtcatgttat gtccattgct gaccacgttg
    10501 tgcaggagtc tcgcgcagcg ggcctgttac cgctcggcgt agaaggtgaa aacagcgccg
    10561 actggattgt cgtggatttg ggcgatgtga ttgtccatgt catgcaggaa gagagccgtc
    10621 gcctgtatga actggaaaaa ctctggagtt aatgcgtgaa gctgcaactt gtcgccgtgg
    10681 gaacgaaaat gccggactgg gtacaaaccg gttttaccga gtacctgcgt cgttttccga
    10741 aagatatgcc cttcgagctg attgaaattc cggccggcaa acgcggcaag aatgcggaca
    10801 tcaagcgcat actcgacaaa gagggtgagc agatgttggc ggccgcaggc aaaaaccgca
    10861 ttgtcatcct cgatattcca ggcaagccct gggatacgcc gcagttagcc gctgagctgg
    10921 aacgctggaa gctggatggt cgcgacgtaa gtctactgat tggcgggcct gaagggttgt
    10981 cgcctgcctg taaagcggcg gctgagcaga gctggtcgct gtcggcgctt accctccccc
    11041 atccgctggt tcgcgtgctg gtcgcagaga gtctgtaccg ggcgtggagc atcaccacca
    11101 accatcctta tcaccgtgag tgataaggga gctttgagta gaaaacgcag cggatgaaac
    11161 tacagaactc ttttcgcgac tatacggcag agtccgcgct gtttgtgcgc cgggcgctgg
    11221 tcgccttttt ggggattttg ctgctgaccg gcgtgcttat cgccaacctg tataatctgc
    11281 aaattgttcg ctttaccgac taccagaccc gctctaatga aaaccgcatt aagctggtgc
    11341 ctatcgcgcc cagccgcggc attatctacg accgtaacgg tatccctctg gccctcaacc
    11401 gcactatcta ccagatagaa atgatgccgg agaaagtcga taacgtgcag caaacgctgg
    11461 acgctttgcg cagcgtggta gatctgaccg atgacgatat tgctgcattc cgaaaagagc
    11521 gcgcacgttc acaccgtttc acctctattc cggtgaaaac taacctgacc gaagtacaag
    11581 tagctcgctt tgccgtcaat cagtaccgtt ttccgggtgt cgaagttaaa ggctataaac
    11641 gtcgttacta tccttacggt tcggcgttga cccacgtcat cggatatgtg tcgaaaatca
    11701 acgataaaga cgtcgaacgc ctgaataatg acggcaaact ggccaactat gcggcaacgc
    11761 atgatattgg taagctgggc attgagcgtt actatgaaga cgtgctgcac ggtcagaccg
    11821 gttatgaaga ggttgaagtt aacaaccgtg ggcgtgttat ccgccagtta aaagaagtac
    11881 caccgcaagc cggacacgat atttacctga cgctggatct caaactccag caatatattg
    11941 aaacgctgct ggcgggtagc cgcgcagctg tggttgttac cgatccgcgt acaggtgggg
    12001 tgctggcgct ggtttccacg cctagttatg acccaaacct gtttgttgac ggtatctcca
    12061 gcaaagatta ttccgccttg ctgaacgacc cgaatacacc gctggtgaac cgcgccacac
    12121 agggggttta tcctcccgcg tctacagtta aaccctatgt ggcggtttcg gcattaagcg
    12181 ccggggtgat cacgcgcaat acgacgctgt ttgacccagg ctggtggcaa ctaccagggt
    12241 cggaaaaacg ttatcgtgac tggaaaaaat ggggccacgg gcgtctgaat gtcacaagat
    12301 cgctggaaga atctgcggat accttcttct atcaggtggc ctacgatatg gggatcgatc
    12361 gcctctccga atggatgggt aaattcggtt atggtcatta caccggtatc gacctggcgg
    12421 aagaacgttc cggcaacatg cctacccgcg aatggaaaca gaaacgcttt aaaaaaccgt
    12481 ggtatcaggg tgacaccatt ccggttggta tcggccaggg ttactggaca gcgaccccaa
    12541 tccagatgag taaggcactg atgatcctga ttaatgacgg tatcgtgaag gttccgcatt
    12601 tgctgatgag caccgccgaa gacggcaaac aggtgccatg ggtacaaccg catgaaccgc
    12661 ccgtcggcga tattcattcc ggttactggg agctggcgaa agacggtatg tacggtgttg
    12721 ctaaccgccc taatggtacg gcgcataaat actttgccag cgcaccgtac aaaattgcgg
    12781 cgaaatctgg taccgctcag gtcttcggtc tgaaagcgaa cgaaacctat aatgcgcaca
    12841 aaattgccga gcgtttacgt gaccacaaac tgatgaccgc ctttgcgcca tacaacaatc
    12901 cgcaagtggc tgtcgccatg attctggaga acggcggcgc gggtccggcg gttggtacgc
    12961 tgatgcgcca gatcctcgac cacattatgc tgggtgataa caacaccgat ctgcctgcgg
    13021 aaaatccagc ggttgccgca gcggaggacc attaatcatg acggctaatc cgaataaaaa
    13081 aacattctgg gataaagtcc atctcgatcc cacaatgctg ctgattttac tggcattgct
    13141 ggtttacagc gccctggtta tctggagcgc cagcggtcag gatattggca tgatggagcg
    13201 taaaatcggc caaatcgcga tgggtctggt catcatggtg gtgatggcgc aaattcctcc
    13261 acgcgtttat gaaggctggg ccccctatct ctatatcatc tgtattattt tgctggtggc
    13321 ggtagatgct ttcggagcca tctctaaagg cgctcaacgc tggctggacc tcggtattgt
    13381 tcgttttcag ccgtcggaaa ttgccaaaat agccgtacca ctgatggttg cgcgctttat
    13441 caaccgcgac gtttgcccgc catcgttgaa gaacactggc atcgcgctgg tgctgatatt
    13501 tatgcccacg ctgctggtgg ctgcacagcc tgacctggga acatcaatcc tcgttgcgct
    13561 ttccggtctg tttgtactgt tcctctctgg ccttagctgg cgtctgattg gcgtcgcagt
    13621 agtgctggta gcggcgttca ttccgattct atggttcttc ctgatgcatg attaccagcg
    13681 ccagcgcgta atgatgctcc tagacccgga atcagaccca ctcggcgcgg gctatcacat
    13741 tattcagtct aaaattgcta ttggctccgg cggattacgc ggcaaaggct ggctgcacgg
    13801 cactcagtca cagcttgaat ttctccccga acgccatact gactttatct tcgcggtact
    13861 ggcggaagag ctgggattag tgggcattct gattctgctc gctctctaca ttctgctgat
    13921 catgcgcggg ctgtggatag ccgccagagc gcaaaccacc tttggtcgcg tcatggctgg
    13981 tggcttaatg ctgatattat tcgtttatgt cttcgtaaat attggtatgg taagcggtat
    14041 tctgccggtt gtaggggttc cgctcccact ggtcagttat ggaggatcgg cgctaattgt
    14101 gctgatggct gggttcggga ttgtaatgtc aatccacacc cacaggaaaa tgttgtcgaa
    14161 aagcgtgtaa gaggtgcgca atgcgtaagc agtggctcgg gatctgcatc gcggcaggaa
    14221 tgctcgcggc atgtacaagc gatgatggtc agcaacagac ggtaagtgta ccgcagcctg
    14281 cggtatgtaa cggccccata gttgaaatta gcggggcgga cccgcgtttc gaaccactga
    14341 acgcgacggc aaatcaggat taccagcgcg acggtaaaag ctacaaaatc gtgcaggatc
    14401 cgtctcgatt tagccaggcg ggactggcgg caatctatga tgccgaacca ggcagtaacc
    14461 tgacggcctc tggcgaagct ttcgatccga caaagctgac ggcggcccat ccaacgcttc
    14521 cgatccccag ctacgccaga atcactaacc tggctaacgg gcgaatgatc gtggtgcgca
    14581 ttaatgatcg cggcccttac ggcaacgacc gcgttatttc gctttctcgc gcggcagctg
    14641 accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga tccgattatt gtcgcccagg
    14701 atggttcgct ttctggtcct ggtatggctt gtaccacagt cgccaaacag acttatgccc
    14761 tgcctgcacc tcccgattta agcggtggcg cgggaacaag ttcagtgtct ggcccgcagg
    14821 gtgacattct tccggtcagt aattcgacgc taaaaagcga agatccgacc ggcgcgccgg
    14881 taaccagtag cggtttcctc ggcgctcaaa cgaccttagc gcctggtgta ctggaaggca
    14941 gcgaaccgac gcctgcgcca cagcccgttg ttacagcttc gtcgacaacg cctgcaacct
    15001 cgcctgcaat ggtgacaccg caagccgcct cgcaaagcgc cagcggcaac tttatggtgc
    15061 aagtcggggc cgtaagcgat caggctcgtg cgcaacagta ccaacagcaa ctgggacaga
    15121 agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt ctggcggatc cagcttggcc
    15181 cattcgccag caaagccgaa gccagtacct tgcagcaacg tttgcaaacc gaagcccaat
    15241 tacagtcatt tattactacc gcgcagtagc gtaaagcagg catctgatgt gtcaatttct
    15301 gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc tgccggtagt gcatttgcta
    15361 tagtagggca cttttttaat tccatcacgg atgtcgtagt tcagaccatg aataccattt
    15421 tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct
    15481 ctgctgcaca tgccgatgac ctgaatatca aaactatgat cccgggtgta ccgcagatcg
    15541 atgcggagtc ctacatcctg attgactata actccggcaa agtgctcgcc gaacagaacg
    15601 cagatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc
    15661 aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat
    15721 gggccaccgg taacccggtg tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc
    15781 aggttccggt ttctcagctg atccgcggta ttaacctgca atcgggtaac gatgcttgtg
    15841 tcgccatggc cgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct
    15901 acgttaacgc actgggcctg aaaaataccc acttccagac ggtacatggt ctggatgctg
    15961 atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcattg atccgtgacg
    16021 taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc
    16081 tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac
    16141 acactgacaa agcaggttac aaccttgttg cttctgcgac tgaaggccag atgcgcttga
    16201 tttctgcggt gatgggtgga cgtactttta aaggccgtga agccgaaagt aaaaaactgc
    16261 taacctgggg cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt aaagagttcg
    16321 cctcagaacc ggtttggttt agtgattctg atcgcgcttc gttaggggtt gataaagacg
    16381 tgtacctgac cattccgcgt ggtcgcatga aagatctgaa agccagctat gtgctgaaca
    16441 gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc aacttccagc
    16501 ttgatggcaa aacgatcgag caacgcccgc tggttgtgtt gcaagaaatc ccggaaggta
    16561 acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaaa
    16621 aattgaacac ttgaaagtgt aatttccgtc cccatatact aagcatcagt aaaaaaactc
    16681 ccaccttctg gcgggagttg ctatttaatt acgttacgcc ggagctgaca tgaaaaccaa
    16741 acttaacgaa ctgcttgaat tccctactcc ttttacttac aaagttatgg ggcaggcgtt
    16801 acctgagctg gttgatcagg tgattgaagt ggtacagcgc catgcgccag gtgactacac
    16861 cccaacggta aaaccaagca gcaaaggcaa ctaccactcg gtatctatca ctatcaacgc
    16921 cactcatatc gagcaggttg aaacactgta tgaagaactg ggcaaaatcg atattgtccg
    16981 catggttctg taactcgctt ctccgttacc cggctcctgg tcgggtaact ctcctctcct
    17041 cgctgtgata tacttttccc ccacttttac tcattctcca cggagatgcc gttttgtatc
    17101 aggataaaat tcttgtccgc cagctcggtc ttcagcctta cgagccaatc tcccgggcta
    17161 tgcatgaatt caccgatacc cgcgataata gtacccttga tgaaatctgg ctggtcgagc
    17221 actatccggt attcacccaa ggtcaggcag gaaaagcgga gcacatttta atgccgggtg
    17281 atattccggt gatccagagc gatcgcggtg ggcaggtgac ttatcacggg ccgggacaac
    17341 aggtgatgta tgtgttgctt aacctgaaac gccgtaaact cggtgtgcgt gaactggtga
    17401 ccttgcttga gcaaacagtg gtgaataccc tggctgaact gggtatagaa gcgcatcctc
    17461 gggctgacgc gcctggtgtc tatgtcgggg aaaagaaaat ttgctcactg ggtttacgaa
    17521 ttcgacgcgg ttgttcattc cacggtctgg cattaaacgt caatatggat ctttcaccat
    17581 ttttacgtat taatccttgt gggtatgccg gaatggaaat ggctaaaata tcacaatgga
    17641 aacccgaagc gacgactaat aatattgctc cacgtttact ggaaaatatt ttagcgctac
    17701 taaacaatcc ggacttcgaa tatattaccg cttaattcca tacatcaatg gcccaattta
    17761 cactgggtca ttactcataa tacctgctaa tgctttacat ctccttcgcg tttgtatatt
    17821 ttctttatca cttgcagaaa tgcggtgcca cgccttgctc cgtaactcac cccattgatt
    17881 aacctattga aatagttgat tatggtgagc aaaagcgttc cgttccaatt agacataaga
    17941 ttattgtaat tttttaaata ttatttttcc atgaataaaa ttggagtaag tgcgtggata
    18001 gtaataatca aattgaaccc tgtttaagca gaaagtcgtc agaaggtaaa ccacaaatat
    18061 ttacaacctt acgaaatatt gatcttaacc ttctgactat ttttgaagct gtatatgtac
    18121 ataaagggat cgttaatgca gcgaaagtgc ttaatctgac cccctcggca atcagtcagt
    18181 ctattcagaa actgcgcgtt atattccctg acccattgtt tattcgcaaa ggccagggtg
    18241 tcactcctac cgcatttgcg atgcatctac atgagtatat cagtcagggc cttgagtcca
    18301 ttcttggcgc gctggatatc gaaggaagct atgataagca acgaacgata actattgcga
    18361 ccactccctc ggtcggagcc ctggtccttc ctgtcatcta tcgggcgatt aaaactcact
    18421 atccgcagct tttactgcgc aacccgccca tcagcgacgc ggaaaaccaa ctcagtcagt
    18481 ttcaaaccga tctcatcatc gataacatgt tttgcaccaa tcgtacggtg caacatcatg
    18541 ttctgttcac cgacaatatg gtgttaattt gccgcaaggg aaatccacta ctctctttag
    18601 aagatgacag agagactatc gacaacgctg cgcatgtact tctgttaccg gaagggcaaa
    18661 atttcagcgg tctgcggcag agagttcaag agatgtttcc ggaccggcaa atcaatttca
    18721 ccagctacaa cattttgaca atcgctgcac tggttgccaa cagtgacatg ttagcgatta
    18781 ttcccagccg tttttataac ctgtttagtc gttgctggcc gctggaaaaa ctgccttttc
    18841 cgtccttaaa tgaggagcaa atagatttct ctatccacta caacaaattc agcctgcgtg
    18901 atcccatcct gcacggagta atagatgtta tccgtaatgc attttgaaaa agtattgtta
    18961 cagccagatc acagtgcaga acgataaaat ggcacaatcc aacaacaatt ttacattttc
    19021 gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc gttaattcaa
    19081 aaatagttga taattacaac aatctattga attgaaacgc tttccttcgt aattcgcaac
    19141 tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa ataccgcgat
    19201 gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga agccctgctg
    19261 cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat ccagggcatc
    19321 aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc ctgccctaac
    19381 ctggcggaat gcttcaacca cggcacagca acgtttatga tcctcggcgc tatttgtacc
    19441 cgccgttgtc cgttctgtga tgttgcccac ggtcgcccgg tagctcctga tgccaatgaa
    19501 ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt tatcacctcc
    19561 gttgaccgtg atgacctgcg cgatggcggt gcccaacact ttgcggattg tattactgcc
    19621 attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt ccgcggtcga
    19681 atggatcgtg ctctggatat tctgactgca acgcccccag atgtgttcaa ccataacctg
    19741 gaaaacgtac cgcgtattta ccggcaggta cggcctggcg cagattacaa ctggtcgctg
    19801 aagctgctgg aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc tggtctgatg
    19861 gtgggtctgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct gcgccgtcat
    19921 ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa gccgccatca cctgccggtt
    19981 caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct ggcgatgggc
    20041 tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt accacgccga tttgcaggcg
    20101 aaagggatgg aagttaagta atcctgtaat aattacttac atctgtctga taaaaaaccc
    20161 gctttttgaa gcgggttttt tgtatcgaac aagatattga gggagcgtcc tgctcgccac
    20221 gtcactcttt atgagagagc ttttcagcct gaagatcaac gtctgcgcct tttttcgccg
    20281 cagcatcgtc atcattcatc gccttcttga accctttaat ggccgctcca aggtctccgc
    20341 ccagcgtacg taacttctta gtcccaaaca gcagaacgac cagcgccgca actaccagca
    20401 gtttggtaat actaatctca cccatagata ccttcttgac atataaccgg caacagaacg
    20461 cgccttaaaa accagagaat attaacggtc attcgacgcg cgcacacaat agcaatctgt
    20521 aacaaggtga atcaagcgcc gagtaaaaaa acatcataat aattgcggcg gcgcaaagcg
    20581 acggttgttt aagatgggca gttgcgcgcg cacctgacgc acacgttcgg gcgtcacttc
    20641 cgccataatg agtgcaggca tttctgacgc tgccgcaatg gtgacgccaa agggatctat
    20701 aatccggctt tgcccgatat ttttgttccc gcactccccc gccgccacca tataacaggt
    20761 ggtatccagc gcacgagcgg caagcaacgt tgaccagtga tgctctttga gcggcccacg
    20821 aacccaggcg gcaggaagta ccaggatttc agctccctgt aaagcctgtg ccagcgccag
    20881 ctctggaaag cgtaagtcat aacaggtcat cagaccgacc ttcatccctt ccacctccag
    20941 taacggagct atttcattac cagcatcaac atggcgtgat tcctgaatgg caaatgcatc
    21001 atagagatgc agtttggcat aacgggcgac gatgtttcct gcctgaagtg ccaccagcat
    21061 attccatgcg cgccccggcg ttgaaggaag atgaatcgtc agaattgtcg tcatcatgtt
    21121 acgtttactt tctcgccgta aacgtccgag gaattcgcct tccagcagct gtgctgattt
    21181 aaccgataga tctgcatcat gatcatcgcg cgccagcaat gcttccggca ggacaaacag
    21241 cgatacgtcg ttttccgccg cctgcgccat caacgaggca caaatctcag cgttcttttc
    21301 ccacacagat gtaacagcaa actgtcctgc agcaaccagc attgccacct cctgtcagag
    21361 tcgtaaaatt cgccaacgac gattattcgg cgttacactt atcactcata caaatcaaat
    21421 agcaggattt tgcagtgtta caacttcttt tagcagtttt tattggcggt ggtacgggaa
    21481 gcgtagcgag atggctgtta agtatgcgat ttaacccgct gcatcaggcg attccgttgg
    21541 ggacgctggc agcaaacctg attggggcat tcatcatagg aatgggattc gcctggttca
    21601 gcaggatgac gaacattgat ccagtgtgga aagtattaat caccaccgga ttttgtggcg
    21661 gtctaacaac cttctcaaca ttttcggcag aagtggtgtt tttgttacaa gagggccgct
    21721 ttggctgggc attactgaac gttttcgtca accttctggg gtcttttgcc atgaccgcac
    21781 tggcattctg gctgttttcg gcctcaaccg cacactaaag accaaaaaaa acccgctgat
    21841 taagcgggtt ttgaattctt gctgacgtat cttacagagc gattacgttt gcagcagaag
    21901 ggcctttggc accgttagtg atttcgaact ctacgcgctg accttcagca agagttttaa
    21961 aaccattagt ctggattgca gagaagtgta cgaacacgtc tttgctgccg tcttccggag
    22021 taatgaaacc gaatcctttg gactcattaa accacttaac gttaccttta atcttagaca
    22081 tcaaaattac ctttacatga aaaatagaca caaatgctgt gtcggttatc agtacaccaa
    22141 ttgtggtacg ctttgtccag tgcaacttta ctaaaaagtg ataaaagtcg cgttattttt
    22201 gatgttgctt attttagtta ttgattttaa agaagttact aaaacttcat ttgcctcaaa
    22261 actgaaagcg catccaggca aagtacacat tgccattgtt gtaggtaccc ggaatgtagg
    22321 tcatctgaaa agtcactggg ccataaccca cggaggccaa tggcagtaga accgggagag
    22381 ggatgtaatt ccagttatcg cgtgccgtta cgccagcggt gaatcccaga cctaaatgaa
    22441 aattttcatc cgccagcggt cgccaggtac tttcccatcc gtatccggca atcggttccc
    22501 atttgttcca cgagtcctta aatgccatgg catacaggcc atgccagttt cctttttcat
    22561 cccaacgcga caggccaaaa ccgccacccc acggtcgctc gttatagcga tcggtttttt
    22621 ctttgtcgta agcgaaacgt gcatgccaag tgatggcagg aatatataaa tcataatgtt
    22681 caggctgttg ccaggtttgt gcaatatttt ctctaaacgt tgtcatcctc tcatctgcgt
    22741 tagcaaaaac tttaccaacg ctgattaact gaataaaaac aaaggaaaag atagcgacat
    22801 atttactcac gttcatttgt gaccataaaa catttatcaa aaatctacta ctagcatagc
    22861 aaagctacaa ttaacataac cttaatagac ccaacataaa gaataatctg aatagctgct
    22921 ctatttattt ccgcttgttc aagttaaata tttccgcgca tcgattaaag atgatgcgtc
    22981 gttttctgcg atgggaatag tcaaaaaaga aaaaccaagt cttttttgat gacaaaatgc
    23041 aatcaaggaa aaattatttt atttttttaa cgagatacaa caatcatctt aacgaagtat
    23101 ataatatata tcgtcaaccc atgctcatat tagattattt tttgagcgca cagccgcagc
    23161 taacaccatt gcaattaaca aatttgcatc aatccaccat caatttacac acattattat
    23221 gtgataattg ccaaccgcta aatatgcgtt ttgttatcta tgtataaaaa cagcaacttc
    23281 aatgtcttaa tggcagtttt tcttgatttt aatcagcatt catcgccaat ttattgggca
    23341 tattttttcc ttaagcttta ggaagttttt attatttact ttgggcctgg agacaggaaa
    23401 aattatgctg acattcattg aactccttat tggggttgtg gttattgtgg gtgtagctcg
    23461 ctacatcatt aaagggtatt ccgccactgg tgtgttattt gtcggtggcc tgttattgct
    23521 gattatcagt gccattatgg ggcacaaagt gttaccgtcc agccaggctt caacaggcta
    23581 cagcgccacg gatatcgttg aatacgttaa aatattacta atgagccgcg gcggcgacct
    23641 cggcatgatg attatgatgc tgtgtggctt tgccgcttac atgacccata tcggcgcgaa
    23701 tgatatggtg gtcaagctgg cgtcaaaacc attgcagtat attaactccc cttacctgct
    23761 gatgattgcc gcctattttg ttgcttgtct gatgtcactg gccgtctctt ccgcaaccgg
    23821 tctgggtgtt ttgctgatgg caaccctgtt tccggtgatg gtaaacgttg gtatcagtcg
    23881 tggcgcagca gctgccattt gtgcctcccc ggcggcgatt attctcgcac cgacttcagg
    23941 ggatgtggtg ctggcggcgc aggcttccga aatgtcgctg attgacttcg ccttcaaaac
    24001 gacgctgcct atctcaattg ctgcaattat cggcatggcg atcgcccact tcttctggca
    24061 acgttatctg gataaaaaag agcacatctc tcatgaaatg ttagatgtca gtgaaatcac
    24121 caccactgcc cccgcgtttt atgccatttt gccgttcacg ccgatcatcg gtgtactgat
    24181 ttttgacggt aaatggggtc cgcaattaca catcatcact attctggtga tttgtatgct
    24241 gattgcctcc attctggagt tcctccgcag ctttaatacc cagaaagttt tctctggtct
    24301 ggaagtggct tatcgcggga tggcagatgc gtttgctaac gtggtgatgc tgctggttgc
    24361 cgctggggta ttcgctcagg ggcttagcac catcggcttt attcaaagtc tgatttctat
    24421 cgctacctcg tttggttcgg cgagtatcat cctgatgctg gtattggtga ttctgaccat
    24481 gctggcggca gtcacgaccg gttcaggcaa tgcgccgttt tatgcgtttg ttgagatgat
    24541 cccgaaactg gcgcactctt ccggcattaa cccggcgtat ttgactatcc cgatgctgca
    24601 ggcgtcaaac ctgggtcgta ccctatcacc cgtttctggc gtagtcgttg cggttgccgg
    24661 gatggcgaaa atctcaccat ttgaagtcgt aaaacgcacc tcggtgccgg tgcttgttgg
    24721 gctagtgatt gttatcgttg ctacagagct gatggtgcca ggaacggcag cagcggtcac
    24781 aggcaagtaa atagtaatgc cggaggcaag tttcctccgg ctttatcatg tcacccactg
    24841 tggtatatgc gttgtggtct gccaactttg ccgtgaacaa tttcagcaat aatcagatgg
    24901 cggctggcgc aatattcaag ataacgcctg gcagtggtgc ggctgatggt cagtgcctgc
    24961 gccaccgttt ctgccgtatg ttgcacacca ggctctttaa acagttttcg caccgcgttt
    25021 agcgtcaagg ggtcaatgcc ggtcggcagt tcgtctttgg gttcaccgcg agcataggca
    25081 ttaaacatct catcaatttg cttctggctg gcgctatcaa tactttccag catatgttta
    25141 cactggcgga aacgggttag cgtttgcccc agtgtattga ttcacttgaa gtacgaaaaa
    25201 accgggagaa cattggatta ttcgggatct gatgggatta gatttggtgg ggcttgcaag
    25261 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    25321 ttgtttcatt gaaaaaacga aaaacaaaaa cactataaat ca
//
LOCUS       DAJTXY010000048        24806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTXY010000048 DAJTXY010000000
VERSION     DAJTXY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.048"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(56..889)
                     /gene="fdhD"
                     /locus_tag="OO566_002088"
     CDS             complement(56..889)
                     /gene="fdhD"
                     /locus_tag="OO566_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6605378.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1083..4133
                     /gene="fdnG"
                     /locus_tag="OO566_002089"
     CDS             1083..4133
                     /gene="fdnG"
                     /locus_tag="OO566_002089"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1668..1670,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6605379.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMFSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGITLTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4146..5048
                     /gene="fdoH"
                     /locus_tag="OO566_002090"
     CDS             4146..5048
                     /gene="fdoH"
                     /locus_tag="OO566_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6605380.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5045..5680
                     /gene="fdoI"
                     /locus_tag="OO566_002091"
     CDS             5045..5680
                     /gene="fdoI"
                     /locus_tag="OO566_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6605381.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5677..6606
                     /gene="fdhE"
                     /locus_tag="OO566_002092"
     CDS             5677..6606
                     /gene="fdhE"
                     /locus_tag="OO566_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6605382.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6936..7178)
                     /locus_tag="OO566_002093"
     CDS             complement(6936..7178)
                     /locus_tag="OO566_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605383.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7396..7614)
                     /locus_tag="OO566_002094"
     CDS             complement(7396..7614)
                     /locus_tag="OO566_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6605384.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(8293..9426)
                     /locus_tag="OO566_002095"
     CDS             complement(8293..9426)
                     /locus_tag="OO566_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605385.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKP
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATVVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            complement(9596..9934)
                     /locus_tag="OO566_002096"
     CDS             complement(9596..9934)
                     /locus_tag="OO566_002096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605386.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(10524..11465)
                     /gene="fabY"
                     /locus_tag="OO566_002097"
                     /gene_synonym="yiiD"
     CDS             complement(10524..11465)
                     /gene="fabY"
                     /locus_tag="OO566_002097"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6605387.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(11510..11947)
                     /gene="dtd"
                     /locus_tag="OO566_002098"
     CDS             complement(11510..11947)
                     /gene="dtd"
                     /locus_tag="OO566_002098"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6605388.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11944..12816)
                     /locus_tag="OO566_002099"
     CDS             complement(11944..12816)
                     /locus_tag="OO566_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6605389.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12810..13409)
                     /gene="yihX"
                     /locus_tag="OO566_002100"
     CDS             complement(12810..13409)
                     /gene="yihX"
                     /locus_tag="OO566_002100"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6605390.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13508..14292)
                     /locus_tag="OO566_002101"
                     /pseudo
     CDS             complement(13508..14292)
                     /locus_tag="OO566_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(14326..15222)
                     /gene="yihV"
                     /locus_tag="OO566_002102"
     CDS             complement(14326..15222)
                     /gene="yihV"
                     /locus_tag="OO566_002102"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6605391.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGVKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            16155..17045
                     /gene="yihT"
                     /locus_tag="OO566_002103"
     CDS             16155..17045
                     /gene="yihT"
                     /locus_tag="OO566_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6605392.1"
                     /translation="MPLGAASREVYSQARASGGFAMLAVDQREAMRMMFAAAGAPAPV
                     ADSVLTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNG
                     IPVDSVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVS
                     IIEPVVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRL
                     NDHINMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDV
                     CAPKLQQLGDIVDEMMAKRR"
     gene            17062..18303
                     /gene="yihS"
                     /locus_tag="OO566_002104"
     CDS             17062..18303
                     /gene="yihS"
                     /locus_tag="OO566_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6605393.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            18441..19343
                     /locus_tag="OO566_002105"
     CDS             18441..19343
                     /locus_tag="OO566_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR6605394.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            19542..21578
                     /gene="yihQ"
                     /locus_tag="OO566_002106"
                     /pseudo
     CDS             19542..21578
                     /gene="yihQ"
                     /locus_tag="OO566_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            21624..23009
                     /locus_tag="OO566_002107"
     CDS             21624..23009
                     /locus_tag="OO566_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6605395.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            23052..24455
                     /locus_tag="OO566_002108"
     CDS             23052..24455
                     /locus_tag="OO566_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6605396.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            24523..>24806
                     /gene="ompL"
                     /locus_tag="OO566_002109"
     CDS             24523..>24806
                     /gene="ompL"
                     /locus_tag="OO566_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR6605397.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGL"
BASE COUNT         6142 a         6381 c         6091 g         6192 t
ORIGIN      
        1 attcacgaaa tgaataagtc ctgcaattta atatatatcg caggacttat tttaattaat
       61 tgctcaaacg ctgcggatgg gtataaaccg ttgcccgacc cggtttacaa aaacctacca
      121 gtgtcagatt acagcgctca gccacttcta cagcaagcgt ggtcgcggca gacaccgcaa
      181 acaaaatctc tacgccgcac atcgccgact tttgcaccat ttcataactg gcacgactgg
      241 aaaccagtac cgcaccttgc tgccagcttt ccccttcttg tgaccggcgg cctaacagtt
      301 tgtccagcgc cacatggcga cccacgtctt catgcccgcc gaccagttcg ccagatggca
      361 acatccaggc agcggcgtga gtacaaccag tcagttgccc cactggctga aaatcgttga
      421 gatgacgtaa tgcatcatcc agtttgttga gatcaaacgt ctgggtgaac ggtagcggct
      481 gcaccggttt tccgatgtca ttaagttgct ccacgccgca tacgccacag cccgtacgtc
      541 ccgccagcgc ccggcggcgc tccttcaacc ccataaagcg gcggctggaa agctcaattt
      601 gtacttcaag accattacag gaaggaacga catccatgcc gaagatatcg cgcggacttt
      661 ctattatccc ctcggaaagc gaaaaaccga gcgcaaagta ctcaaggtct ttgggcgacg
      721 ccatcatcac cacatgcgaa atgccgttgt agaccagcgc aacgggaact tcttccgcga
      781 cctcatctaa ccgtgggtgt tgtaaatcat cacgccgcca taactcaatt tgacgaacac
      841 ctgtgatatt tgtcacattt tcgatttctt ttcgctgtgt ttttttcact ttctcttaac
      901 cagataagaa cacacacacc aacattatgg tattctgtta caaacccttc ctagatggag
      961 ggaaattgag ccaattctgg acctttgcgg ccccttccgc aaagaaaaat aactcccact
     1021 ccctgcacac gcagcaagcg aatgtaaatg ggacgtgaca atgtcgaaac aaggagcaat
     1081 ccatgcaggt cagcagaagg cagttcttta agatctgcgc tggcggtatg gcaggcacca
     1141 cggcagcggc actgggtttt gcacccagcg tagcactcgc ggaaacccgg cagtataaac
     1201 tgctgcgcac ccgcgaaacc cgtaatacct gcacctattg ttccgtaggc tgtgggctgt
     1261 tgatgttcag cctcggtgac ggagcaaaaa acgccaaagc atctatcttc catatcgaag
     1321 gcgatccgga tcacccggtc aaccgcggtg cactttgtcc gaaaggcgct ggcctggtgg
     1381 atttcatcca ctccgaaagc cgtctgaagt ttccggaata ccgtgcgcca ggttctgata
     1441 aatggcagca aatcagttgg gaagaggcgt ttgatcgcat cgccaaactg atgaaagaag
     1501 accgcgatgc taactacatt gcgcaaaacg ccgaaggcgt gactgttaac cgctggctct
     1561 ccaccgggat gctgtgtgct tccgcgtcga gtaacgaaac cggctattta acgcaaaaat
     1621 tctcccgcgc gctgggtatg ctcgcggtcg acaaccaggc gcgtgtctga cacggaccaa
     1681 cggtagcaag tcttgctcca acatttggtc gcggtgcgat gaccaaccac tgggtcgaca
     1741 tcaagaacgc caacctcgtc gtggtgatgg gcggtaacgc cgctgaagct cacccggtcg
     1801 ggttccgctg ggcgatggaa gccaaaattc acaacggcgc gaagctgatt gtgatcgatc
     1861 ctcgctttac gcgtacggcg gcggtagctg actactatgc ccctattcgt tccggtactg
     1921 acattgcttt cctgtcaggc gtattgctgt acctgctgaa caatgaaaaa ttcaaccacg
     1981 aatacaccga agcctatacc aacgccagcc tgatcgtgcg tgaggattac ggctttgaag
     2041 atggcctgtt caccggctac gacgcggaaa aacgcaagta cgataaatcc tcctggactt
     2101 atgaactgga cgaaaacggc ttcgccaaac gcgataccac gctgcaacat ccgcgctgcg
     2161 tgtggaactt gctgaaacag cacgtttccc gctacacgcc agatgtggtt gaaaacatct
     2221 gtggtacgcc aaaagacgcg ttcctgaaag tctgcgaata catcgcagaa accagtgctc
     2281 acgataaaac tgcctcgttc ctgtatgccc tcggctggac gcaacactcc gttggtgcgc
     2341 aaaacattcg tacgatggcg atgatccagc tgctgctcgg caatatgggg atggccggcg
     2401 gcggcgttaa cgccctgcgc ggtcactcca atattcaggg gctgacggac ctggggctac
     2461 tgtcgcagag tctgccaggt tacatgacgc tgccaagcga gaagcagacc gatctgcaaa
     2521 cctaccttac cgccaacacg ccaaaaccgc tgctggaagg ccaggtaaac tactggggca
     2581 actacccgaa attcttcgtc tctatgatga aggccttctt tggtgataaa gcgacggcgg
     2641 aaaatagctg gggctttgac tggttgccga agtgggataa aggctacgac gtcctgcaat
     2701 acttcgagat gatgaaagag ggcaaggtca atggctatat ctgccagggc tttaaccctg
     2761 ttgcctcatt cccgaacaaa aacaaagtga tcggttgtct gtcgaaactg aagttcctcg
     2821 tcaccatcga cccactgaac actgaaacct ctaacttctg gcagaaccac ggtgagctga
     2881 acgaagttga ctcgtcgaag atccagaccg aagtgttccg tctgccatcg acctgcttcg
     2941 cagaagagaa cggttcaatc gttaactcag gtcgctggtt gcagtggcac tggaaaggtg
     3001 cggacgcccc ggggattacg ctgactgacg gtgagatcct ctccggtatc ttcctgcgct
     3061 tgcgcaagat gtatgccgaa cagggtggcg cgaacccgga tcaggtgctg aacatgacct
     3121 ggaactacgc cattccgcat gagccgaagt cggaagaagt agcgatggag agcaacggta
     3181 aggcgctggc cgatattacc gatccggcaa ccggtgcggt tatcgtcaag aaaggccaac
     3241 aacttagctc gttcgcccaa ttgcgtgatg acggtacaac ctcctgtggc tgctggattt
     3301 tcgccggtag ctggacgccg gaaggcaacc agatggcgcg tcgtgataac gccgatccgt
     3361 ctggcctcgg taacacgctg ggctgggcat gggcatggcc gcttaaccgc cgcattctgt
     3421 ataaccgcgc ctccgcagat ccgcagggta acccgtggga tccgaagcgt cagttgctga
     3481 aatgggacgg cactaagtgg accggctggg atattccgga ctacagcgca gcgcctccgg
     3541 gtagcggcgt cgggccgttt atcatgcagc aggaaggcat ggggcgtctg tttgccctcg
     3601 ataagatggc ggaaggtccg ttcccggaac actacgagcc gtttgaaacg ccgctgggaa
     3661 ctaacccgct gcatccaaac gttatctcga acccggctgc acgtatcttt aaagacgacg
     3721 ccgaagcatt gggtaaagcc gataagttcc cgtatgtcgg aaccacctat cgtctgaccg
     3781 agcacttcca ctactggacc aaacacgcgc tgttgaacgc gattttgcag ccagagcagt
     3841 ttgtggaaat cggggagtcg ctggcgaata aacttggcat tgcccagggc gataccgtga
     3901 aagtctcctc caaccgtggc tatatcaaag ccaaagcggt ggtgaccaaa cgtattcgca
     3961 cgctgaaagc gaacggcaaa gatatcgata ccatcggtat tccgattcac tggggctatg
     4021 aaggcgtagc taaaaaaggc tttattgcca atactttaac gccattcgtt ggtgatgcga
     4081 acacgcagac gccggagttt aagtccttcc ttgtgaatgt ggaaaaggtg taacggagac
     4141 gacatatggc ttatcaatct caagatatca ttcgtcgttc cgcgactaac ggtctgaccc
     4201 ccgcgcctca ggcgcgggac ttccaggaag aagtggcgaa actcatcgac gttaccacct
     4261 gtatcggctg taaagcctgt caggtggcgt gttcagagtg gaacgacatt cgcgataccg
     4321 tcggcaataa cattggggtg tacgacaacc ccaatgattt aagcgccaaa tcgtggacgg
     4381 taatgcgctt ctcggaagtg gagcagaacg acaaactgga atggctgatc cgcaaagacg
     4441 gctgtatgca ctgttccgat ccaggctgcc tgaaagcgtg cccggcggaa ggtgcaatta
     4501 ttcagtatgc caacggtatc gtcgacttcc agtccgagca gtgcattggc tgcggttatt
     4561 gcattgcggg ctgtccgttc gacattccgc gcctcaaccc ggaagacaac cgcgtctaca
     4621 aatgtacgct gtgcgttgac cgcgtggtgg ttgggcaaga accagcctgc gtgaagacct
     4681 gcccaacggg cgcgattcac ttcggtacga aagagtcgat gaaaacgctg gcgagcgagc
     4741 gcgttgctga gctgaaaacc cgcggttacg acaatgcggg tctgtacgat ccggcaggcg
     4801 tcggtggtac acacgtcatg tacgtgctgc accatgctga caagccaaat ctgtatcatg
     4861 gcttgccgga gaacccggaa atcagcgaaa ccgtgaaatt ctggaaaggc atctggaaac
     4921 cgctcgcggc tgttggcttt gcggctacct ttgcggccag tatcttccac tacgtgggtg
     4981 tcggtccgaa ccgtgcggat gaggaagaga ataatctgca cgaagagaaa gacgaggagc
     5041 gcaaatgaaa cgacgtgaca ccatcgtgcg ctacaccgcg ccggaacgta tcaaccactg
     5101 gatcaccgcc ttctgcttca tcctggcggc ggtgagcggg ctgggctttt tgttcccgtc
     5161 cttcaactgg ttgatgcaaa tcatgggcac accgcagctg gcgcgaattc tgcacccgtt
     5221 tgtcggcgtg gttatgtttg cctcgttcat catcatgttt ttccgttact ggcatcaaaa
     5281 cctaatcaat cgggatgata tcttttgggc gaagaatatt cgtaagatcg tcgtcaacga
     5341 ggaagtaggt gacaccgggc gttataactt cggtcagaaa tgcgttttct gggcggcgat
     5401 tattttcctg gttctgctgc tggtgagcgg cgtgattatc tggcgtcctt attttgcgcc
     5461 tgctttctca atcccggtga tccgattcgc gttaatgctg cattcatttg ccgcagtggc
     5521 gttaattgtg gttatcatgg tgcatatcta cgccgccctt tgggtgaaag gcacgattac
     5581 cgccatggtg gaaggatggg ttaccagcgc ctgggcgaag aaacatcacc cgcgctggta
     5641 ccgtgaggtc cgcaagacaa cggaaaagaa agctgaatga gtattcgcat aatcccgcaa
     5701 gatgagctgg gttcgagcga gaaacgtacg gcgaatatga ttccgccgtt attgttccct
     5761 cggctcaaga atttatacaa ccgccgcgcc gagcgtctgc gcgagctggc agaaaataat
     5821 ccgctgggtg attacctgcg ctttgctgcg cttatcgccc acgcccagga agtggtgctg
     5881 tacgaccatc cgctggagat ggatctgacc gcgcgcatta aagaagccag cgcacagggc
     5941 aagcccccgc tggatattca cgttctgccg cgtgataagc actggcaaaa gctgctgatg
     6001 gcgctaattg ctgagctgaa acctgaaatg agcggcccgg cgctggcagt gattgagaat
     6061 ctggagaagg catcgactca ggagctggaa gatatggcca gcgcgctgtt tgcctctgat
     6121 ttctcgtccg tcagcagcga taaagcgccg tttatctggg ctgcgctgtc gctctactgg
     6181 gcgcagatgg ccaatctgat ccccggcaaa gcccgcgctg aatacggcga acaacgtcaa
     6241 tattgcccgg tatgtggctc tatgccggtg tccagcatgg tgcaaattgg caccactcag
     6301 ggtctgcgtt acctgcactg caacctgtgt gaaaccgaat ggcacgtagt gcgcgtaaaa
     6361 tgcagcaact gcgagcagag cggcaaactg cattactggt cgctggatga cgaacaggcc
     6421 gcgattaaag ccgaaagctg cgatgactgc ggcacttacc tgaagattct ttatcaggaa
     6481 aaagaaccga aagttgaagc cgtggcagat gacctcgcct ctctggtact ggacgcgcga
     6541 atggagcaag aaggctacgc ccgtagttcc attaacccgt tcctgtttcc gggtgaagga
     6601 gagtaatctc tgacgttttc cgggtggcgc aggtcgcccg gattacagct catagggtgt
     6661 gacgacacca tctctcgtat tccgcgttac ccattcaaga tcatgccgca attgtctgtt
     6721 tgcctcgttc ttccctccaa cacatcgata aggttatggg tatgtcactg atgacaagcg
     6781 tatgattgcc tgatgcgctg cgcttatcag gcctaccaat aaacttcaat atattgaatt
     6841 tgcatgactt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa caacgcgcac
     6901 gttgtcagca atctgattga catcattttt tcccattacc attcctcgcg cagcttacgc
     6961 tgatattcga caccatcttc ctcacaccct tgccagatgc caggaacact ggttcttgct
     7021 gtttgcgatt gatttatcag gtattgatct accgcttccc ttaataggtc cgcacgagga
     7081 agattacgct gcacctcgag atcatcaagt tgcttaatca cctcattcga taaatcgagt
     7141 aaaattctgc ccatatctat acctgccaga gagttcataa atatctcgcc aatatcattt
     7201 ttgaatctat ggagagaaaa gtacccttgc cgaatcttta aagaaagctc atttacgcat
     7261 cattttttat ttttgcgagg cgttttccag atacgatgta acagttgcag ttattataag
     7321 ttcaagtaaa acagccttgt attaatacca gaataagggc atctgttttt tatattcaag
     7381 aatgaaaaat ttttttcatt ccttatgctc cttacactga tcatcaacac caactacctc
     7441 ccccttttgc ctgcctccct ttgctttacg caccggaaat tgctgccgct ccagatattg
     7501 ctcaaccgct tcgcgtaaaa ttgcagatcg tgaaacctta cgccagcccc gaagcttgtt
     7561 cagccgttta attgcctctt ctgataaatg aagaaaaacc gtattcatcg ccattaccac
     7621 caccttacat atattactca ttaatgtatg tgcgaaaatt acgccgaaaa agcatttaaa
     7681 aaaatgcgga tttttgcatg tcattttgtt tttgcgaggc gttttccaga aataataaga
     7741 gcgttgcagt tgtttcattt attactcaaa aatggtttag ttatcgccca ggatgagtta
     7801 tgggtgctat gaatatatta tattcacgga tatttatttg cataatctga atggtgtagc
     7861 cgaaaaacgt cagcatccta acagcaccag gatttaaggt gaaattaatc tttcatcaca
     7921 gaccggtcag tactgccacc aaccggtctt tacttgctca tcgttataat tttgttggta
     7981 acagttcggg aaacaaggaa ttccttccgg aacacccccg gcatcatccg atagcattga
     8041 cgtcaatcat cagcgcgacg atgagttttt ttaatcgcat catagggtgg cgacgttcag
     8101 cacgattacg ggtcatagca aaactcctga gcaggcggcc actatgccgc tttcactgga
     8161 gttctgaata ctggtacttt ctgtatggct tcgccatgcc cctcgcggct aagtacctgc
     8221 acacatctct ctttgtatgt catttttgca atgcattgtc cgaattctga taatcctaat
     8281 gagttttact cgtcagactc ccatccctac ggtatacgag ttatattcat gaatgaaatc
     8341 gacattttta aaaaacgaat cttgtaaaac atcagtgttg atattacctt gcaatccaac
     8401 attgaatttg ttagagacat gaagaactcg aatatcaaaa aggtttttta acatcattaa
     8461 ttcttcgggt gttaatgaat cgcaactaaa atatccctgt tgatcaaaaa gattttctat
     8521 ttctccacta tcggcgaggc gtttttcaag gagtgtttgc aacatcgagt tgccaaggac
     8581 aatggatatc gtgcgatctt tcgcagattt acaccccatc acaacggcaa cattcgtatc
     8641 cccctcaaga gtttcattta tcgccgtcgc taaaatggtg gtcaaacacg caaacggaac
     8701 tgtatttctt tcgtgattta atttatctac cagttcattc catttatcaa aagccgggct
     8761 atatttgttg cgcacagaaa tatccgactg gttaaacaat tcagccatgc gattttttat
     8821 catctcgata tccggcgaac cagaaagact ttgcgcataa tacggtcctg atgatgttgc
     8881 atacattaat gccaagccca caactgtcgc catgatgggc gcagccacgg ttttggcaag
     8941 ggcgacgctg cgatggctat gcgcttcaca ggctaaagga acatattcca ctgactgcgg
     9001 taattcctta catgcctccc tcatcattgc ggtatgcttt acaaacaacg gtttttcact
     9061 gcccaaaaaa actttctgct taattgcact aagaaaacgc aaatcaaaaa taattgtttt
     9121 tggtttatta ttttttggca catttcctat tatcccgcta agaaccttcc ttgctgcatg
     9181 gagatttttt tgcacagcac ataacacaat tgatgaagcc ttctccactg cattattgat
     9241 tttaatttta aaatccgcat gatatacgtt gacgggcaat gcaagtatat ctggacctac
     9301 atcttttgct gagtatttat tggtgatcga cggacgtgaa cccacgagaa aacttagctg
     9361 caaattatgc tctatattat cagctgtttt tgtggtgtgg ttaaacatag agatgccacc
     9421 tttcattctt tcacacatat cctcaacttt ttcacttcgt tcttgtggca atttggcttt
     9481 aacattaatt tgatgatttt tacccgtgca ttccttaact acaaggggaa tgttatctaa
     9541 agtgccatac aggctaataa cgtcaccagg atttttatca tgtaagtaag tttgattaat
     9601 acttgaagca ttcacaaaga gggtcaatat tataactgtt tataacttca cctacatcaa
     9661 gtgtgtagtg cttactctgg agactgccat ccttatttaa ttgtatatca atcaatatat
     9721 ccccggactt tgtcaccata tctttgccat aaaccatttg catagccatt tcgaggagat
     9781 ttttatctaa ctgattgcgg gcgttgagta agcgattacc aaataattta tatactgttc
     9841 ccgtcattgc cagcgctgcg ctttctggta ctttgcgttt ccacagtgag gtttctctat
     9901 ttttaaacag gtcatattca ttattactcg acaaaccatg cttcagttcg ttaacacaat
     9961 cgcacagttg ctcctcacct tccattttaa ggcaaagtga aaattgattt aatggcgttt
    10021 acttcggtga tttattactc attagttgtt tttctaaatt aacaacagaa taactttctt
    10081 gtttaatgag attaatattg gcaaccatta tagcctcctt cccacggttt aagagcaaac
    10141 atacaacata atgattcata acatacagag cagtaaatga atgatactga caatgtatgc
    10201 cagcccatta tctttgcata atgtttgtga gctatagatt ttaaaaatat ttatgaaaaa
    10261 agtatggcac cagagggggt tttgactatc aaaaatcgct caaacctgaa ctaagatgat
    10321 gagttaagca ttatctgcaa taattgccac tcagctctac aaataatatt taaatgctcc
    10381 tgaacagtga tgtagcaaga cacgtatggt gccaacgaag aataaaaatt tataaagttc
    10441 ttattcaata taaccggaat agtcagggac tgatgtaaat gaccggaatg aatgagagag
    10501 gcagcaccgc ccctctccat aagctactct tcttcgttcc cgccctcttc atacgggcca
    10561 aatggcttcg cgggcagaac gatatacgtg ccttcaaaca ctgcacccgg cgtctcgtcg
    10621 ccaaagattt cgacctgcat ctgcacccgt gcttttcgtc cgcgcgccag acggtcgaga
    10681 tcgccgctta aggcaccaag gtcggctacc gcatggggtt taccgctaat cggtttgctg
    10741 tagcggatat gcgcatccgc cagaataatc gttccgccga ggtggcgttc acgcagcatc
    10801 agccagataa gcccccaacc ggtgagcgtc gccagcgaga ataaactccc ggcaaacagc
    10861 gtatggtgcg gattctgatt gccggtttcc ggcatggtgg tgataaattt ttgcccggta
    10921 tattgttgaa tacgcacgcc cattttttca ctaagcggga tatgttcgta ccacgcctgt
    10981 tgcagctgcg cgcaccagtc gccgcgatgc aaaatatcat ccagagtggc gacgggctta
    11041 atcatcaaaa aatggcgaat cggcgtggtg gttggcgtgg tgatttctcc ctgattaaca
    11101 aaccccagct tggcgaaaaa ctccaccgcg tcttcacggg cgctacaggt cacgcgctta
    11161 acgccttcct gacgcgccac cgactccagg gtcatcgcca tcagcgttcc taaccctttg
    11221 tcctgcacgt ccgggtgaac ggccataaag cgaatggacg cttcattgtc ggcattaata
    11281 tacaatcggc ctaccgccac cagattaccc tgctcgtcga cgaccatctg gtgatgcgcc
    11341 atcgcatccc acgcgtcgcg ttccgaacct tttggttgat gcaggggctt acgcaacatt
    11401 tcccagcgaa actgatagta acgctctaac tcttcttctg tttgtggaac ctgaaggtga
    11461 tacatagcga tactctctct tgtttcccgt gacaaccctg gaagctggct catacctgca
    11521 accagaatgt cacggggcca tcattgacca gcgatacctg catatcggca gcgaagcgtc
    11581 ctgtttgcgt gttcatctct tgctggcggc agcgttcgac gaaatagtca tataacgcct
    11641 ctgcgcgatc cggtgatgcg cctttggaga aacttgggcg catcccccgt tcggtatctg
    11701 cggcgagggt aaactgggaa accaccagta cactgccgcc cgcctgttgc acgttgagat
    11761 tcatcttgcc ttcggcatcg ctaaagatgc ggtagccgag cacacgctcg cacagacggt
    11821 ttgctttctg ttcgtcgtca tccttttcga cacccaataa caccaaaagt cccgcgccaa
    11881 tttcgcccgt cacttctccc tccacggtga cgctggcacg ggttacgcgt tgaattaatg
    11941 caatcatggt tcgtcgtctt cttcttgttc agctgcttgt tttagtttgc ggtattcccc
    12001 gagagtgaca gtaatttccg cgccaagcaa gacgatacac catgtccagt agacccaaac
    12061 aaagagaatg gggatcaccg ccagcacacc gtaaatgagc tgatatgacg ggaacatggt
    12121 gatataaagc gcgaaacctt tctttcctgc ttcgaacagg agtgcggcga caaacgcgcc
    12181 gacaatcgcg tcgcggttag gtacgcggat ggtaggaaca atgctgtaca gcaaccagaa
    12241 ggagatccac gacaacagca acggaaaaat acgcagcacg ttgtcgatga cagtattgag
    12301 atcgctcgcc cagcgcaggg agagcaaata ggaactgatc gccagactgg cccctgccag
    12361 cagcggcccc agcgttaaaa tcatccagta cacggcgaac gagtaaattt ttggtcgcgc
    12421 tcgtttactg cgccagatgg tattcaacgc gctatcgatg gagtacatca acaataacgc
    12481 ggtgacgatc agcccgcacg ccccaacggc ggtcatcttg ttggaattag caacaaattg
    12541 ttcgatatac cgctgaataa catcgccagt agcaggcaga aagttggcaa aaataaagtg
    12601 acgtaactga atgctgacgt cggaaaacat gggaaaagcg gcgaaaagcg caaaaacaac
    12661 ggcaaccagc ggcactaatg agagcaacga cacataggca aggttacctg ccagggttgt
    12721 catgttgtcc tcatcaatac gttgccagag tagttttagc caggcccata gtggacgggt
    12781 acggtgcctg gctttgtcct gaatggtttt tagcataaca ccttcgcgaa atagtccggg
    12841 atggtggttt tatctttcac cagaatactg gtaatgccta gctgattagc tccttctata
    12901 ttatcggcgt tatcgtcgaa aaagaccgta tcgctgggtg aaaaaccttc cgcctgcaaa
    12961 acatgctggt aaattcgtgc ttcaggtttg cgcatcccca gatcttgcga cagatagata
    13021 tggtcagcag catcacgaat ttccgggtat tcttccggcc agaaggtggt atgcaggcgg
    13081 ttggtattgg aaagcaccac cacgcgatgc ccctgctcac gcagtttatg catgatggcg
    13141 atcacttccg ggcgcagcgc aacaaacacc gcctgccagc cgtgagagaa ctgctcgtag
    13201 cttagcggta gagccatctc atgacacagc gcctctgcga acgcttcgtc gctaatttcc
    13261 ccacgctcat gctgatgaaa cgcctccccc atatgaaaac tcttcttaag cgatgccagc
    13321 ggaatacgcg ttaaatcgct ccaggctccc agcacacggt taaagtcgat atcgacaatc
    13381 acattaccta aatcaaagat atagagcatt tttgcctcct ttcgcgccac aagaaattaa
    13441 ctgtagcggg aaaggatggg tttggctatg tggcaaggta tgaagccagg ttaacggtca
    13501 caaaaatcta cgcgtcttcc tggggaagaa tgatttcaat ttggctgtct tgtaagcgtg
    13561 attttagcgc agcgggcggc agctcgtcgg taaagagcgc agtgacctgt gccacgttac
    13621 caatttcaac cgccgccgaa gcatgatact tagtgtgatc ggcgaccagc agaatatttc
    13681 tcgcgtgcgc catcatcgtt ttcaccacgt tagcttcgtt tacatcaaac tccatcaacg
    13741 cgccatcgct ctcaatcgcc ccaacgcttg ttaccagata atcagcacga aaatcagcca
    13801 caaaggacgc cgctgaaggg ccaatgatcc cgctattatg agagcgcaac gtaccgccgg
    13861 gcaccatcac ttcaaagcgc gggttgtggt aaagaatatg cgccacacgc aggctgttgg
    13921 tgattatccg caaatgatta tggttaagta acgcccgggc aacatgctca acagtcgtac
    13981 caatggtgat aaatattgtt gaaccatcag gaatatagtc tgccaccgct tcggcaatcg
    14041 cttttttttc tcggtttgcg aaacctcacg ctgctcgaac gccgtattaa cgacgctgga
    14101 agcccgaccc gcgccaccgt gatggcgcgt aatcaggcct tgctcgctta atttacgaat
    14161 atcccggcgg accgtctgcg tggagacatc cagcagattt gccagctcat caatattcat
    14221 atacccacgc tcggcgatca gcatgaggag ttggtcgtgc cgcgggttac cggttagttc
    14281 ggtaaggctc atgaaaattc ctcgaaaaac catcaccctg gcattttata caaaaagtga
    14341 caaaaaagat cgggtttgat cacagtcagg gatcccggct cgtccaccgg gacgtgtgca
    14401 ttttaacgcc gctacaccgc tggcgaagcg gactgactcc gctaaatccc cacttgttgc
    14461 cagcgccacc gccaaagcgc cgtgaaaaac atcacccgca cctgtggtat ctaccacatc
    14521 aactttgaag gccggttgat gctgacgccc accattttcc agccagtcgc agcctgcgct
    14581 accctgggtc acatagacat gaccatttgt gagcgtttgt gcctgtttta gcgcactggc
    14641 catctctttc acgcccgtta agcgcgccag acccggttct gaaaaggccg cgtgatcgct
    14701 taatgccacc agctcactga tatcctgcgg cgtaatgtcc ccgtccagaa cggtcatcac
    14761 acccgcctga cgggccaggg tgaaggcttt tttaacgccg tcgtgccagc gtacatctgc
    14821 cagcacaaca tcccactgag agaaatcaat ttcctccaac cactctgcgt caggcagcag
    14881 atccgggctg gggtagttaa tgattatccg ctcgcctttg gtatccacca tgatggcgga
    14941 ttgcgaagat ttcgcctggt tataccgttt ggtgtaacgg gtgttaacgc cccaggattc
    15001 cagctctgct agcaggctgt tgcccgcgtc gtcatcacct acgcgaccaa taaaatcgac
    15061 ctgcgccccc agccttgccg ccgcaaccgc tgccgtcgcc gctggcccgc caccaacttc
    15121 cgtataattt ctcgccacgt atttaccgct ctccgtcggt aacccttcca cgtaatagat
    15181 gcgatccatc acggttatac ctacacaagc aacacgaatc atggtcattc cttaaacatt
    15241 ttgggatgcg ttcattttca tttcaccaat tgtttaaaaa gtgacggctg tcaatttttt
    15301 gacgataaat tacaaatgcg atcaaaaaca gacaagcaaa gcatgtcata aatgacaaaa
    15361 agtgacatca ctgtattcag gagagggtta tggcagcaat cgcgtttatc ggtttaggac
    15421 aaatgggttc gccaatggcg agcaatttat tgcagcaagg gcatcaactt cgcgtctttg
    15481 atgtgaacgc cgaagctgtg cggcatctgg tagacaaagg tgcgactccc gccgccaacc
    15541 cggtgcaggc cgctaaagat gccgaattta tcattaccat gttgccgaat ggcgatctgg
    15601 tgcgcaacgt gttgttcggt gaaaacggcg tttgcgaagg cttatctacc gatgcgctgg
    15661 tcattgatat gtccaccatc catccgctgc aaaccgataa attgattgcc gatatgcaag
    15721 ccaaaggctt cagcatgatg gatgttccgg taggccgtac ttctgcaaat gccattaccg
    15781 gtactctgtt actgctggct ggcggcaccg ctgaacaagt tgaacgtgcc acaccgatcc
    15841 tgatggcgat gggcagtgag ttgatcaacg ctggcggtcc gggcatgggg atccgcgtta
    15901 agctcatcaa caactacatg agcatcgcgc tcaatgcgct ttcggcagaa gccgccgttt
    15961 tgtgcgaagc cctgaatctt cccttcgatg ttgccgtcaa agtgatgagc ggtaccgccg
    16021 ccggtaaagg tcacttcacc acttcctggc caaacaaagt cctcagcgga gatctttctc
    16081 ccgccttcat gatcgatctt gcccataagg atcttggcat cgcccttgat gtcgccaacc
    16141 agctgcatgt gccaatgccg ctgggggccg cctcacggga ggtttatagc caggcgcgcg
    16201 catcgggcgg ttttgccatg ctggcggtcg atcagcgcga agccatgcgc atgatgtttg
    16261 ccgcggctgg ggcacccgct ccggtagccg atagcgtttt aactgatttc aaagttaacg
    16321 ctgcaaaggc cctctcgcct tatgcctcgg cgattctggt agatcaacaa ttctgctacc
    16381 gccaggtggt tgagcaaaac gcgattgcca aaagttgcgc catgattgtc gccgccgatg
    16441 agttcattcc tggcaacggt attccggtcg atagcgtggt tattgaccgc aaaatcaatc
    16501 cgctacagat caaacaagac ggtggcaaag ccttaaaact gctggtgctg tggcgtagtg
    16561 atgaagatgc gcagcaacgt ctggatatgg tgaaagagtt caacggactg tgccactcac
    16621 acggtctggt aagcatcatt gagccagtcg tccgcccacc gcgtcgtggc gataaattcg
    16681 atcgcgaaca agcggtcatc gatgccgcca aagagctggg cgacagtggc gctgacctct
    16741 acaaagttga aatgccgctt tatggcaaag gtccgcaaca agagcttctc tgtgcttcac
    16801 aacgtctgaa tgaccatatc aatatgccat gggtgatcct ctcttccagt gtcgacgaaa
    16861 aactgttccc gcgtgccgta cgcgtggcaa tgacggcggg cgcatcggga ttcctggcag
    16921 gccgtgcagt ctgggcatcg gtcgtcggtt taccagacaa cgagctgatg ctccgtgacg
    16981 tttgcgcacc gaaattacaa caacttggcg atatcgtcga cgaaatgatg gctaaacgcc
    17041 gctaagatga ggataaaagg aatgaaatgg tttaacaccc taagccacaa ccgttggctg
    17101 gaacaggaaa ccgaccgcat ctttgatttt ggtaaaaatt ccgtagtgcc gactggtttt
    17161 ggctggttag gcaataaagg gcaaatcaaa gaagagatgg gcacccatct gtggatcaca
    17221 gctcgtatgt tgcacgttta ttccgttgct gcggcgatgg gtcgacctgg cgcttacgcg
    17281 ttggttgatc acggtattaa agccatgaac ggcgcactgc gtgataaaaa atatggcggc
    17341 tggtatgcct gcgtgaatga cgagggcgtg gtggatgcct ccaaacaggg ctatcaacac
    17401 ttctttgctc tgctgggtgc cgccagcgcc gtcacaacgg gtcacccgga agcgcgcaag
    17461 ctgctcgatt acaccattga gattatcgag aaatatttct ggagcgaaga agagcagatg
    17521 tgcctggaat cctgggacga agccttcagc aaaaccgaag agtaccgcgg cggcaatgcc
    17581 aatatgcacg cggtggaagc tttcttgatt gtttatgacg tcactcacga caaaaaatgg
    17641 ctggatcgcg cgattcgcgt ggcttccgtg attatccacg acgtcgccag aaataatcat
    17701 tatcgcgtta acgaacactt cgatacccag tgggatccgc tgccggatta caacaaagat
    17761 aacccggcgc atcgcttccg cgcgttcgga ggtacaccag gccactggat cgaatggggc
    17821 cgtttaatgc tgcacatcca tgctgccctg gaagcccgtt gcgaacaacc accagcatgg
    17881 ctgctagaag atgccaaagg tctgtttaac gccaccgtgc gcgatgcctg ggcacccgat
    17941 ggcgcggacg ggattgttta taccgttgac tgggaaggaa aaccggtggt ccgcgaacgt
    18001 gtacgttggc ctatcgtcga agcaatgggt acggcctacg cgctctacac cgtcactggc
    18061 gatcgccagt atgaaacctg gtatcaaaca tggtgggagt actgcattaa gtacctgatg
    18121 gactatgaaa atggttcctg gtggcaggag ctggatgcgg acaataaggt catcaccaaa
    18181 gtctgggacg gcaaacagga tatttatcac ctgctgcatt gcctggtgat cccgcgtatc
    18241 ccgttagcgc ccggcatggc tccagcggtt gcagcgggtc tgctggatat taatgcgaaa
    18301 taactgatct gaaaataggg tgcgggtttt cccctcaccc taaccctctc cccaaagggg
    18361 cgaggggacc gtccggtgca gtttttgggt ttcacatcag cctcacggga ggctgtatgt
    18421 cgttaattaa ggtgccttgt atgcaaatca ccaatatgca ttgcagtgga cagaccgttt
    18481 cacttgccgc gggggattat cacgcgacca ttgtgaccgt cggtgctggt ctggctgaat
    18541 taacctttca gggttgtcat ttagtcatcc cgcacaaacc cgaagagatg ccgctggcgc
    18601 atctgggtaa agtgcttatt ccgtggccga accggatcgc caacggctgt taccgttatc
    18661 aggggcagga atatcagtta cccattaatg aacatagttc aaaagccgct attcacgggc
    18721 tgcttgcctg gcgagactgg caaattagtg aattaaccac caccagcgtt accctgacag
    18781 cttttctgcc gcccagttac ggctatccgt ttatgctggc ttcacaagtt gtttattcgc
    18841 tcaacgcaca cacgggtctt tccgttgaga tcgcaagcca gaatatcggc actgtcgcag
    18901 cgccttatgg cgtgggtatt catccttatt taacctgcaa cttaacatct gttgatgaat
    18961 atctgttcca gcttccggca aaccaggttt atgccgtcga tgaacacgta aatccgacaa
    19021 cgcttcacca cgttgatgag ctggatttaa atttcacgca ggcgaaaaaa atagccgcga
    19081 caaaaatcga ccacaccttt aaaaccgcta atgatttgtg ggaaatgacc attacccatc
    19141 cacagcaggc gctttcggta agcctttgtt ccgatcaacc ctgggtgcag gtttatagcg
    19201 gtgaaaaatt acagcgtcag ggactggcag ttgaacctat gagttgcccg cctaacgcct
    19261 ttaattcagg tatcgactta ttattactgg aaccaggaaa accgcaccgc ctgtttttta
    19321 atatttacgg acagagaaaa taacttgtat tgattagcag tcaaaatcat tgaagaacat
    19381 ttcactcgct aaaggcaaca ttatgtatct tcaatttatt aagttaaaaa cagctcagct
    19441 tctgtaaagt ttttatatat cagttttatg agaatcattt tacataagcc tgtaataaca
    19501 ggaaggcaac aatctcgttc tcaactctat ggagattagt tatggatacg ccacgtccac
    19561 agttattaga ttttcaattt catcagaata acgacagttt taccctacat tttcaacaac
    19621 gtcttatttt aacccatagc aaagataatc cttgtttatg gattggctca ggtatagcgg
    19681 atatcgatat gttccgcggt aatttcagca ttaaagataa actacaggag aaaattgcgc
    19741 ttaccgacgc catcgtcagc cagtcaccgg atggttggtt aattcatttc agccgtggtt
    19801 ctgacattag cgccacgctg aatatctctg ccgacgatca gggccgttta ttgctggaac
    19861 tacaaaacga caaccttaac cacaaccgta tctggctgcg ccttgccgct caaccagagg
    19921 accatatcta cggctgcggc gaacagtttt cctacttcga tctgcgcggc aaaccgttcc
    19981 cgctatggac cagtgaacaa ggcgttggtc gcaacaaaca aacctatgtc acctggcagg
    20041 ccgactgcaa agaaaatgcg ggcggcgact attactggac tttcttccca cagcctacgt
    20101 ttgtcagcac gcagaagtat tactgccatg ttgataacag ttgctacatg aacttcgact
    20161 ttagcgcccc ggaataccat gaactggcgc tgtgggaaga caaagctacg ctacgttttg
    20221 aatgtgctga cacatacatc tccctgctgg aaaaattaac tgccctgctg ggacgccagc
    20281 cagaactgcc cgactggatt tatgacgggg taacgctcgg cattcatggc gggacggaag
    20341 tgtgccagaa gaaactggac accatgcgta acgcgggcgt gaaggttaac ggcatctggg
    20401 cgcaggactg gtctgggatc cgtataacct cttttggcaa gcgcgtgatg tggaactgga
    20461 agtggaacag cgaaaactac ccgcaactgg attcacgcat taagcagtgg aatcaggagg
    20521 gcgtgcagtt cctggcctat atcaacccgt atgttgccag cgataaagat ctctgcgaag
    20581 aagcggcaca acacggctat ctagcaaaag atgcctctgg cggtgactat ctggtggagt
    20641 ttggcgagtt ttacggcggc gttgtcgatc tcactaatcc agaagcctac gcctggttca
    20701 aggaagtgat caaaaagaac atgattgaac tcggctgcgg cggctggatg gctgacttcg
    20761 gcgagtatct gcccaccgac acgtacctgc ataacggcgt cagtgccgaa attatgcata
    20821 acgcctagcc tgcgctgtgg gcgaagtgta actacgaagc ccttgaagaa acaggcaagc
    20881 tcggcgagat cctcttcttt atgcgcgccg gttctaccgg tagccagaaa tactccacca
    20941 tgatgtgggc gggcgaccag aacgtggact ggagtctcga cgatggcctg gcgtccgtag
    21001 tcccggcggc gctgtcgctg gcaatgaccg gacatggtct gcatcacagc gacattggcg
    21061 gttacaccac cctgtttgag atgaagcgca gcaaagagct gctgctgcgc tggtgcgatt
    21121 tcagcgcctt cacgccgatg atgcgcaccc acgaaggtaa ccgtcctggc gacaactggc
    21181 agtttgacgg cgatgcggaa actatcgccc attttgcccg tatgactacc gttttcacca
    21241 ccctgaaacc ttatctgaaa gaggccgtcg cgctgaatgc gaagtccggt ctgccggtca
    21301 tgcgcccgct gttcctgcat tacgaagacg atgcacatac ctattccctg aaatatcagt
    21361 acctgttagg ccgcgacatt ctggtcgctc cggtacacga agaaggtcgc agcgactgga
    21421 cgctctatct gccggaggat aactgggtac atgcctggac gggtgaagcg ttccggggcg
    21481 gggaagttac cgttaatgcg cccatcggca agccgccggt cttttatcgc gccgatagcg
    21541 aatgggcggc actgttcgcg tcgttaaaca gcatctaatc acatccgccc gtggggaaac
    21601 tcatgggcaa agggagaaca tcgatgagtc acatcacaac ggaagatcca gcaactttac
    21661 gcctgccctt taaagagaaa ctctcttacg gtattggcga cctggcctct aacatcctgc
    21721 tggatatcgg tacgctttat cttttgaagt tttataccga cgttctgggg ctgccaggca
    21781 cctatggcgg cattatcttt ttgatttcaa aattctttac tgcgtttacc gatatgggta
    21841 ccggcattat gttggattcc cgacgcaaga tcggtccaaa aggtaagttc cgtcctttta
    21901 ttctgtatgc gtcattcccg gtcaccttac tggcgatcgc caactttgtc ggcacaccgt
    21961 ttgatgtcac cggtaaaacg gtgatggcca ctattctgtt tatgctttac ggactgtttt
    22021 tcagcatgat gaactgctcc tacggcgcaa tggtcccggc aatcaccaaa aaccccaacg
    22081 aacgcgcctc actggcggca tggcgtcagg gcggcgctac gctgggcctg ctgctgtgca
    22141 cggtgggatt cgtgccagtt atgaatctta tcgaaggtaa tcagaaactt ggctatatct
    22201 tcgccgccac gctgttttca ctgttcggcc tgctgtttat gtggatctgc tactcaggcg
    22261 tgaaagagcg ttatgtcgaa acccaaccaa ccaatccggc gcaaaagcct ggcctgttgc
    22321 aatctttccg cgcaattgcc ggtaaccgcc cactgttcat tctgtgtatt gccaacctct
    22381 gcaccttagg ggcgtttaac gtcaagctcg ccattcaggt ctattacacc cagtacgtac
    22441 ttaacgatcc catcctgttg tcgtatatgg gatttttcag catgggctgt attttcatcg
    22501 gcgtgttcct gatgcctggc gcagtcagac gttttggtaa gaagaaggtc tatatcggcg
    22561 gcctgctgat ttgggtgctg ggcgatctgc tcaactattt cttcggcggc ggctcggtca
    22621 gctttgtggc gttctcctgc ctggcgttct tcggctcagc gtttgttaac agcctgaact
    22681 gggcgctggt ttccgacacc gtcgagtacg gcgagtggcg caccggcgtg cgttcggaag
    22741 gcacggtcta caccggcttc accttctttc gcaaagtgtc tcaggcgctg gctggtttct
    22801 tccccggctg gatgctgacg caaattggct atgtgccgaa cgtcgcacag gctgaccaca
    22861 ccattgaagg gttacgccag ttgatcttca tctacccaag cgcactggcg gtagtcacca
    22921 ttgtggcgat gggttgcttc tacagcctga acgagaagat gtatgtccgc attgtggaag
    22981 agatagaagc ccgtaaacgc acggcgtaat aataataaac gacgccctgc ggggcgttat
    23041 aaggagtgat tatgtctgac cataatccac tgacattaaa actgaatctg cgggaaaaaa
    23101 tcgcctatgg tatgggcgac gtcggttcga atttaatgct ctgcatcggt actctgtatc
    23161 tcctcaaact ttataccgat gaattaggga tgcctgctta ctatggcggg attatctttc
    23221 tggtcgcgaa gttttttacc gcgtttaccg atatgctcac cggattttta ctcgactcgc
    23281 ggaaaaatat tgggcctaaa ggcaaatttc gtccctttat tttatatgca gcagttccgg
    23341 cggcgttaat tgcaacgctc cagtttatcg ccaccacctt ttgtttgccg gttaaaacga
    23401 cgattgccac cgcgctgttt atgatgtttg gactttcata cagtctgatg aactgctcgt
    23461 atggtgcgat gatcccggca attaccaaaa acccgaatga gcgcgcgcag ctcgcggctt
    23521 accgccaggg tggtgcaacc atagggctat tgatttgtac cgtggcgttt attccgttgc
    23581 agtcgctttt ttctgactca accgtcggtt atgcctgtgc ggcacttatg ttctccattg
    23641 gcggctttat ttttatgatg ctgtgctaca gaggcgtcaa agagcattat gtggacacag
    23701 cgccaaccgg acataaagcc agtattctca aatctttttg cgcgatattt cgtaatccac
    23761 cattgctggt tttatgcatt gctaacctgt gtactctggc ggcatttaat atcaaactgg
    23821 cgattcaggt ctattacacc cagtatgtgc tgaatgatat taatttattg tcgtggatgg
    23881 gattcttcag tatgggatgc atcctcgtcg gcgtattact ggtgccagta actgtaaaat
    23941 gttttggtaa aaaacaggtt tatttagctg gcatggtgct atgggcggtg ggtgatatac
    24001 tgaattattt ctggggaagt aactctttca ctttcgtcat gttctcttgt gtcgcctttt
    24061 ttggcacggc ctttgtaaac agcctgaact gggcactggt accagatacc gtagattacg
    24121 gtgaatggaa aaccggtatt cgtgccgaag gttctgttta taccggttat accttctttc
    24181 gtaaaatttc tgccgcactt gctggcttct tgccaggcat tatgctgacg caaattggtt
    24241 atgttcccaa catcgcgcaa agcgatgcga cattacaggg tttgcgtcag ctcattttta
    24301 tctggccttg tgcactggca attattgctg cattgacgat gggattcttt tacacactca
    24361 atgaaaaacg gtttgcattg attattgagg aaatcaacca acgaaaaaat aaagagatgg
    24421 cgacagaaaa aaaaacggct tccgtaacgt tataaatgtc aatagccgct atttccatcc
    24481 tggtgggtgg cggcctccct acgtttaaaa aatggactta ttatgaaaaa gattaatgca
    24541 ataattttat tatcttctct gacctctgcc tcggtatttg ctggcgcata cgtcgaaaac
    24601 agggaggcat ataatcttgc ctccgatcag ggcgaggtta tgctacgtgt gggttataac
    24661 ttcgatatgg gcgcgggtat tatgttaacc aatacctaca cctttcagcg agaagatgaa
    24721 ctaaaacatg gctataatga aattgaaggt tggtatcctt tattcaaacc aaccgataaa
    24781 ttaaccatcc agcccggtgg cttaat
//
LOCUS       DAJTXY010000049        24598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTXY010000049 DAJTXY010000000
VERSION     DAJTXY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.049"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            67..1144
                     /locus_tag="OO566_002110"
                     /pseudo
     CDS             67..1144
                     /locus_tag="OO566_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1537..1878)
                     /locus_tag="OO566_002111"
     CDS             complement(1537..1878)
                     /locus_tag="OO566_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6605398.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1891..2763)
                     /gene="copD"
                     /locus_tag="OO566_002112"
                     /pseudo
     CDS             complement(1891..2763)
                     /gene="copD"
                     /locus_tag="OO566_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(2767..3141)
                     /gene="yobA"
                     /locus_tag="OO566_002113"
     CDS             complement(2767..3141)
                     /gene="yobA"
                     /locus_tag="OO566_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6605399.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3280..3510
                     /gene="holE"
                     /locus_tag="OO566_002114"
     CDS             3280..3510
                     /gene="holE"
                     /locus_tag="OO566_002114"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6605400.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3612..4268
                     /locus_tag="OO566_002115"
     CDS             3612..4268
                     /locus_tag="OO566_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR6605401.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4292..4954
                     /gene="exoX"
                     /locus_tag="OO566_002116"
     CDS             4292..4954
                     /gene="exoX"
                     /locus_tag="OO566_002116"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6605402.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4951..7011)
                     /gene="ptrB"
                     /locus_tag="OO566_002117"
     CDS             complement(4951..7011)
                     /gene="ptrB"
                     /locus_tag="OO566_002117"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6605403.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7220..7879)
                     /locus_tag="OO566_002118"
     CDS             complement(7220..7879)
                     /locus_tag="OO566_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6605404.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8206..8562)
                     /locus_tag="OO566_002119"
     CDS             complement(8206..8562)
                     /locus_tag="OO566_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6605405.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8629..8919)
                     /gene="yebG"
                     /locus_tag="OO566_002120"
                     /pseudo
     CDS             complement(8629..8919)
                     /gene="yebG"
                     /locus_tag="OO566_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9053..10231
                     /gene="purT"
                     /locus_tag="OO566_002121"
     CDS             9053..10231
                     /gene="purT"
                     /locus_tag="OO566_002121"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6605406.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10286..10927)
                     /gene="kdgA"
                     /locus_tag="OO566_002122"
                     /gene_synonym="eda"
     CDS             complement(10286..10927)
                     /gene="kdgA"
                     /locus_tag="OO566_002122"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6605407.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10964..12775)
                     /gene="edd"
                     /locus_tag="OO566_002123"
     CDS             complement(10964..12775)
                     /gene="edd"
                     /locus_tag="OO566_002123"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6605408.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13010..14485)
                     /gene="zwf"
                     /locus_tag="OO566_002124"
     CDS             complement(13010..14485)
                     /gene="zwf"
                     /locus_tag="OO566_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6605409.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14823..15692
                     /gene="hexR"
                     /locus_tag="OO566_002125"
                     /gene_synonym="yebK"
     CDS             14823..15692
                     /gene="hexR"
                     /locus_tag="OO566_002125"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6605410.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15820..17262
                     /gene="pyk"
                     /locus_tag="OO566_002126"
     CDS             15820..17262
                     /gene="pyk"
                     /locus_tag="OO566_002126"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6605411.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17393..18364)
                     /gene="lpxM"
                     /locus_tag="OO566_002127"
                     /gene_synonym="msbB"
     CDS             complement(17393..18364)
                     /gene="lpxM"
                     /locus_tag="OO566_002127"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6605412.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18484..19806)
                     /gene="mepM"
                     /locus_tag="OO566_002128"
     CDS             complement(18484..19806)
                     /gene="mepM"
                     /locus_tag="OO566_002128"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6605413.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLLFD"
     gene            complement(19822..20754)
                     /gene="znuA"
                     /locus_tag="OO566_002129"
     CDS             complement(19822..20754)
                     /gene="znuA"
                     /locus_tag="OO566_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6605414.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            20833..21588
                     /gene="znuC"
                     /locus_tag="OO566_002130"
     CDS             20833..21588
                     /gene="znuC"
                     /locus_tag="OO566_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6605415.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21585..22370
                     /gene="znuB"
                     /locus_tag="OO566_002131"
     CDS             21585..22370
                     /gene="znuB"
                     /locus_tag="OO566_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6605416.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22517..23527)
                     /gene="ruvB"
                     /locus_tag="OO566_002132"
     CDS             complement(22517..23527)
                     /gene="ruvB"
                     /locus_tag="OO566_002132"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6605417.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23536..24147)
                     /gene="ruvA"
                     /locus_tag="OO566_002133"
     CDS             complement(23536..24147)
                     /gene="ruvA"
                     /locus_tag="OO566_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6605418.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(24286..24351)
                     /gene="yobI"
                     /locus_tag="OO566_002134"
     CDS             complement(24286..24351)
                     /gene="yobI"
                     /locus_tag="OO566_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6605419.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24422..>24598
                     /locus_tag="OO566_002135"
     CDS             24422..>24598
                     /locus_tag="OO566_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605420.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLS"
BASE COUNT         6086 a         6233 c         6047 g         6232 t
ORIGIN      
        1 atataacaca ccatcagcct aatgggcgac aagggagaga cagtccgtta atggagaagt
       61 taaaacatgc agcggaaaaa atacgatccc aatttgccta gaaacttaac atatcgaagg
      121 agagacaaag catattactg gcgcaaccct ctgacgaaag aagaatttac actaggtaaa
      181 atttcaagaa gagatgcagt agcgcaggca attgaagcaa atcattatat atacaaaaac
      241 tactctcctg ctgccctaat tgaaaagctt aaaggggtcg actcatttac tatggcagac
      301 tggattgaac gttacaaaac gattcttata aggagaaaag cgtccagaaa tacttataaa
      361 actcgggtaa atcaactggt gacaataaaa gaaaaattag gaggggtttt actgacagaa
      421 ataaccactc gccatatcgc cgagtttctt gatttgtgga ttgaaggagg gaaaaacacg
      481 atggcaggat caatgcgttc tgtgttatct gatatgttcc gcgaagccat tgttgaagga
      541 cgtatatctc aaaatccagt aacgccaaca agagcaccga aaatagtagt tacaagagaa
      601 cggctgaaac taaagacata caactgcatc agggaggcag cagatcaact tccggcatgg
      661 ttcccattag ctatggactt agcccttgta acaggacaac gtcgcgaaga cataacgaat
      721 atgcggttca gtgatattta tgatgatcgt ctccacatca ggcaaattaa gacaggaatg
      781 atgattgcta tccccctgtc actcagcctt cctgtcgctg gtttacgact tggtgcagta
      841 gttgaacagt gccgcatggt aagtaggggg ggattatcta atcagtgccg ggattagaaa
      901 aaacagccct gacggcagca ttcacccgga cggcctgaca aagaaatttg tcgcagccag
      961 aaaattaaca ggtatcctgt tcagtgaaaa cccaccaact tttcacgaaa tcagaagcct
     1021 ggctggacga ttgtacaaag aaatatgtgg agaagaattt gctcagcgtc tacttggcca
     1081 cacatcggag aagacaacaa aaatgtatct tgatgagaga gaaaaaacgt acttactgct
     1141 ctgattttaa cgtaaatgga ttgttaaatg tattttggtt gtgatataac caaaaaagac
     1201 cggaatacag aaattcgagt aaatttcggg gaatttcggg gagacgtttg caactaattg
     1261 attttaaata caattaaaaa aagaccgaat acgattcctg tattcggtcc agggaaatgg
     1321 ctcttgggag agagccgtgc gctaaaagtt ggcattaatg caggcttagt tgccttgccc
     1381 tttaagaata gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt caataaaaag
     1441 cgcggtggtc atcagcttaa atgttaaaaa ccgcccgttc tggtgaaaga actgaggcgg
     1501 tttttttatt ggaaatcaaa aggctatttt aggtaattaa cagagttttt cagctcgttc
     1561 tataaacggt gccagactca ttttttcgcc gggattgtta ggatcatcaa tctgaatcac
     1621 cgaaatgggt tgggcattgg tcttcccact ggcaacttcc ttttgtgcga tatcgtttaa
     1681 aggatactgc acgagggtac ttgggttgat gacatacaaa gcattacccg gtcggcaagt
     1741 cagcattacc tcttcgcgat taaacgccca tttgtcttta cccacttcaa aacgactcac
     1801 ggttatcacc tgtggagccg ccagcgccgc tgcagaactg gtgagtaaca gaaacgccag
     1861 aatacttttt ttcatcattt tcgtaatcca tcaaaaaggt tcgagagtcg caagcaggct
     1921 gatgatcacc agcactaccg cacctatcgc ccattcgagt ttagtcatcc acacaaacca
     1981 gggtgccgca cgatcttcat cctgcctcat gcgcggtaca agaacataac gattagccag
     2041 cgcaatgacc accataatca tcaccaggat ggctttgagc aataagagtt ggccctagta
     2101 agtcgtaagc gtggggggaa atccggtaat caacaatgca ttaagcacgc cgcttgccag
     2161 tacgccgatc accgcaaaat gcccgcacca ggaaaaacgc atcagcgcct gaatagcctg
     2221 gtgtcgccaa cggcctttga tgagttgcat acaccagagc accggcagta atcccccaaa
     2281 ccacgccgcc gcacaaatca ggtgaatcgc gtgattagtc tgatggattt tcgctgttac
     2341 cccttcattc agcgtcgcat gccccactcc cgccagcaga ataaattgcg cggtagtgag
     2401 cataaacaac aaacgtggca tattccgcgg ttgcataagg gcgacgatca acgttacgag
     2461 ggcgagaaca atttgccata accagacgcc accaaactgc gtttgcaaaa cagcctgcca
     2521 aatgtttggc gaaaatacat ctgtccatcc cgttcccatc aacccgccct gaacggcgag
     2581 cattgcagtg gcactgatca aactccagac ggcagcgtgc tgttgtaaac gcagagaacg
     2641 cttcgtcagc aagcgacgaa tcattaaggg ggccagccag gcaccataca ttgcaaagcc
     2701 aaagaccagc atcaacgagg tgaaatggat aaatcgcagc gcgatccagg taaacgccag
     2761 catgatttat ttcacgctaa aggtgtagtg tcctttcgtt ttatgcccgt ccaccgaaac
     2821 aacatgccag tcaacggtgt aggttccggg tttcagcgaa tcggccagtg gaacaatcag
     2881 ttgcttttgg tcctgctcat tccgcttcgc tggtaatgtt ttaatatttt cgttttttgg
     2941 ccccgtgatt tttgcaccac tgaatcctgt ttcaacgcct tccgagaagt ttaaagtgat
     3001 tgcctgcggg gcagctgtca cttgcgcgtt tgccgcagga tactgatgcg ttaaatgcgc
     3061 atgtgcccag acggaaggtg tcaccaacga agtggtcagg attgccagcg cgtagcgaag
     3121 ggagcgtgca gttgaagcca tattatctat tcctttttgt aataactttt ttacagagca
     3181 taaccttgtc taatgtctga gtcgaggatc atcaattccg gcttgccatc caggctcact
     3241 cttagtaact tttgcccgcg aatgatgagg agattaagaa tgctgaagaa tctggctaaa
     3301 ctggatcaaa cagaaatgga taaagtgaat gtcgatttgg cggcggccgg ggtggctttt
     3361 aaagaacgct acaatatgcc ggtgatcgct gaagcggttg aacgtgaaca gccagaacat
     3421 ttgcgcagct ggtttcgcga gcggcttatt gcccaccgtt tggcttcggt caatctgtca
     3481 cgtttacctt acgagcccaa acttaaataa aacttataca gagttacact ttcttacata
     3541 acgcctgcta aattatgagt attttctaaa ccgcccccat aatttgcagt cattttgaaa
     3601 aggaagtcat tatgtcgttc tggaaagttg cagcagcgca atatgagcct cgtaaaacct
     3661 cactcaccga acaggtagct catcatctgg agtttgtgag ggccgccgcc aggcaacaat
     3721 gtcagctgtt agtttttcca tcgctttctt tactggggtg tgattattcg cgacgtgccc
     3781 ttcctgcccc acccgatctt tcactgttag acccgctttg ttatgccgca acgacctggc
     3841 gaatgaccat cattgccggc cttcccgttg aatataacga tcgctttatt cgtggaattg
     3901 cggtgttcgc accgtggcga aaaacgcctg ggaactacca tcagagtcat ggtgcatgtc
     3961 tgggccgtcg ttccagaacc atcacggtgg tcgatgaaca accgcaaggt atggatatgg
     4021 acccgacctg ttcactgttt acgacagggc aatgtctggg agaacccgac ctgctggcgt
     4081 cggcccgtcg cttacaattt ttttcacatc agtactcgat tgccgtgctg atggcaaatg
     4141 cccgtggtaa cagtgcacta tgggatgaat atggtcgtct catcgttcgc gccgatcgtg
     4201 gttcattatt gttagtcggt cagcgttcat cacagggttg gcaaggcgat atcattccat
     4261 tacgctaggc tttttcggcc tggagcatgc catgttgcgc attatcgata cagaaacctg
     4321 cggtttgcag ggagggatcg ttgagattgc ctctgttgat gtcattgacg gaaaaatcgt
     4381 caaccccatg agcctcctgg tgcgccccga tcgtcctatt agtccacaag cgatggcgat
     4441 tcatcgcatc accgaagcca tggtcgccga taaaccgtgg attgaagatg tgatcccaca
     4501 ctattacggt agtgaatggt atgtcgcgca caacgccagc tttgaccgcc gcgtactgcc
     4561 tgagatgccc ggtgagtgga tttgcactat gaaactggcc cgtcgtttgt ggccagggct
     4621 caagtacagc aatatggcgt tatataaaac acgcaagctc aatgtacaga caccgccggg
     4681 cctgcatcat caccgcgcgt tgtatgactg ttatatcacc gccgcgttgc ttatcgatat
     4741 tatgaacacc tccggctgga cggcagaaca gatggccgat atcaccggac gtccgtcgtt
     4801 gatgacgacc ttcacctttg gcaaataccg tggcaaagcg gtttccgacg ttgccgaacg
     4861 cgatccgggc tatctgcgct ggttatttaa taacctggac agcatgagcc cggagctgcg
     4921 tttaacactg aaacattatc tggaaaatac ttaatccgca ggcgtagtgg gtaatgttcc
     4981 ctgcgccagc gcgaccagaa aagcatattc catcgctacg ccttcgtacg atttaaagcg
     5041 gccagattta ccgccatggc ctgagtccat gtcggtacag agcaataaaa gatggtcatc
     5101 ggttttcagc tcgcgcaatt tagcgaccca ttttgccggt tcccaatatt gcacctgaga
     5161 atcgtgtaaa ccggtcgtta ccagtaaatg cggataagcc tgtgcggtga cgttgtcata
     5221 cgggctgtag cttttcatgt actcgtaata ttgcggatcc tgcggatttc cccactcttc
     5281 aaactcacca gtggtaagag gaattgattc atcaagcatc gttgttacaa catcaacaaa
     5341 cggtacctgg gcgataacgc cgtggaataa ttccgggcgt tgattaattg caacgcccat
     5401 caacatgccc cccgcacttc cgcccatcgc ataacagagc gaaggagagc catagcccag
     5461 ttttaacaat gcatcgcagg catcaagata atcattaaac gtatttttct tcttcagaaa
     5521 tttaccgtct tcgtaccatt gttgccccag ctcaccaccg ccgcgaacat ggacaatggc
     5581 gtagacaaag ccacgatcta acaaactcaa gcggctaaaa ctgaaatcgg catcaatact
     5641 tgcgccgtaa gaaccatagc catacaccag caacgggttg tgtcctttgc gaaaatgttt
     5701 gcgatgatag accagcgaaa ccggaacttc gacgccatca cgggcgacta tccacaggtg
     5761 ttcactgcgg taattcgccg catcaaaacc gggcacttct gtttgtttta atacacgacg
     5821 ctcaccggta tccatatcca gttcaaacaa agtgtctggt gtggtcatgg aagaataacc
     5881 ataacgcaat cgcgcggttt caggttctgg attgtaggca atccaggtca cataggccgg
     5941 atcatcaaag gcaatgccaa tgacttcccg ggtcttgcgg ttaatttggc gcaaactggt
     6001 taacccgcgc tgacgctctt caaccaccag ccagtcggta aacagcgtaa acccttccag
     6061 catgatgttt tcacgtggcg gaattaactc ttcccactgt tgctcatcac gcatacgggt
     6121 acggtataag ccaaagtttt tgccgtggcg attggaacgc agataaaaac gatgctggta
     6181 gtgatcaagg ctgtattcgt gatctttgcg gcgcggcaga aaaacaaacg gctcggcatc
     6241 ggccatttcc gcgtccagca ggcgaacttc actggtggtg gcgctggcca gatgaatgac
     6301 gacatagtgc ttcgacgtcg ttttatgcag gctgacataa taggtatcgt ctttttcttc
     6361 gtagatcagt gtatcttgcg atgctggcgt accgatggcg tgacgccaga cctgataagg
     6421 cagcagcgtc accggatgct tgcgaacata gtagaaagtc caggagtcat ttgcccagac
     6481 aaagctgggt tcaacgttat ccagcagttc cgggtaccag ttacctgttt ccagattacg
     6541 aaaacgaatg ccgtactggc gtcgggaaag aaaatcttct gccagcgcca taatggtgtt
     6601 atcgggcgta atcgccattc cgcccatcga ataaaactca ctatgagctg cgcgcttgtt
     6661 ggcatcgagc aatgtttccc actcatccca ctcttcgctg aatgccgatt gacgctggta
     6721 gatagcatat tcacagcctg gttcataaat atgccgatag cggtagccat tttttatgta
     6781 gggcgcagaa acttctcttt gcggaatgcg gtcgatgatt tcctttaaga tgcgatcctg
     6841 caaggcttgt tgtgaggcca tcacccgatg accgtaacta ttttcttgtt gcaggtagtc
     6901 caggacttct ggctgagaac gcgtatcgtc ccgcagccag tagtaattat cgatgcgcgt
     6961 atcgccatga agcgtcatgg cgtggggaat gcgggcggct tttggtagca tgttattgtt
     7021 ctttctggtt gaaacatctt ataagggtgg caaaactcac cggggatgca agcgaaacag
     7081 ggtcgtcatt gcttagatga tgacaggtaa tggcgcggat atcgaatgtt atgcagacag
     7141 agaaaatcag cctgttcaaa tggctgtgcg gttctggata gcccgaaata gtcaacttca
     7201 ggctatccag agagcggaat tattccgcca aagtgcgttt ttgctgttcg agatcgcgtt
     7261 caatgccttc acgaacatcc tgggggattt tcagcgcgtc acccagtgca ttcaggtaac
     7321 tgcgttccat aaaatggtca atatcaatag ccgcgcagct caggaaatag atttccagcg
     7381 cctcttcttc attgcggacc ccggtagcca ggcgttgtgg atccagcggt tgttcgattg
     7441 cctgctcaat gagtacacgc ccctgctctt ccacgccggc ttcacgcaat tgctggtcga
     7501 ttgccgcacg ttcattggca tcaatatgac catcactttt agcggcaaag accagcgcaa
     7561 ggatcaaacg ttccgtacgc tcatccagcg gcgtactttg cgcgccaaac tgcggttcgt
     7621 cctgatgcgc cgcgcgaatt ttatctttgt atttattcca cagcaccgta cccgctaccg
     7681 ctccgccgcc aaccagtaac gcgttggtgc catatttcgt aagaagttta cgtgctgatt
     7741 tatttgcgac cagcagccca gccagtccgc ctaatgcgcc tggcactaac agtttgccca
     7801 atccctgatc cgcagacgag gaggtagaag aactgctttg cccaagcagg gattgcagtt
     7861 gatttaacca gttagccata tttgctcctc aataaccatt tttagtgcct gtagcatagc
     7921 ggatgaggat gtcagaaaat gtatatcgag acaaaagatg cgcaaataag cggggcgcta
     7981 ggccgttatt caaagaaatt cgcgccaggt aaggtattta ctgtatatca ggtgataagg
     8041 gtattttagg tgaaagtagt ttgtcgtttt gagtggcatt gtttgatgct gattgcccga
     8101 tgcgacgctg ttgcgtcttc tcaggcctac gattgtggtg cgttctggga cggacaaggc
     8161 actcgcgccg catccgccaa cggtcaatcg aattatcaga agccattaac gccgctgata
     8221 ttccgccatt cccgctttgc agtccacgct gacctggtaa tgaatatcgg cacttttacc
     8281 acgcacggtt agcggtactg accatttatc atctttaccc tgaatgtcct gcaaactgac
     8341 ccaggccacg ggatcggcct gaccgacaat tttttgatca tctgcccagc gcgccacgcg
     8401 attttgttga taatcacgtt ttacgctcgc ggcaattccg gtagcatcca gaccttcaca
     8461 ctttgggaaa gtgaccgact tgctggtttc attattggca gcgaaaactg atgcgcaggc
     8521 agaaaccaac aaccgcccta aaaacgcccc tctttttttc atgtttttct ccatagcaca
     8581 atgattcagg agaaagcatg gtacaaattg tcaggagcgc aagttgcttc aggccgcgtg
     8641 tgaggcatct tcctcttcgg attcagcacc gacgaccagt ggggacggta atttgccggt
     8701 tttcagaatg gtgctcagca tatctttttg ttctgccagc catagcgaaa gggcttcacg
     8761 ttgctcgtct tccatttgca ctggagaatt tgtcagctag gtgtcaagca gatccgccgt
     8821 atcaagcatt ttgtcataag catcggcttc ctttttgctg gtaaacgaca ttttctcttc
     8881 gccctcacga atgactacgt atttaacttc aaccgccatt tgcagcctct cataataact
     8941 gtgattttat acagtatatt tcttttcggt tgagaaatca acatcagcaa taaagacaca
     9001 cgcaaacgtt ttcgtttata ctgcgcgcgg aattaatcag gggatattcg ttatgacgtt
     9061 attaggcact gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct ccggtgaact
     9121 gggtaaagaa gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg ccgtcgatcg
     9181 ctatgccgac gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta atatgcttga
     9241 tggtgatgca ttacgccgtg tggttgaact ggaaaaacca cattatatcg tgccggagat
     9301 cgaagctatt gccaccgata tgctgatcca acttgaagag gaaggactga atgttgtccc
     9361 ctgcgctcgc gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc tggcggcaga
     9421 agagctgcag ctgcccactt ccacttatcg ttttgccgat agcaaaagcc ttttccgcga
     9481 ggcggttgcc gccattggct atccctgcat tgtaaaaccg gtgatgagct cttccggcaa
     9541 ggggcagacg tttattcgct ctgcagagca acttgctcag gcatgggaat acgctcagca
     9601 aggcggtcgc gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt ttgacttcga
     9661 aattaccctg ctaaccgtca gcgcggtgga tggcgtccat ttctgtgcac cagtaggtca
     9721 tcgccaggaa gatggcgact accgtgaatc ctggcaacca cagcaaatga gcccgcttgc
     9781 ccttgaacgt gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg gttatggtct
     9841 gtttggtgtc gagctgtttg tctgtggtga tgaggtgatt ttcagtgagg tctcccctcg
     9901 tccacatgat accgggatgg tgacgttaat ttctcaagat ctctcagagt ttgccctgca
     9961 tgtacgtgcc ttcctcggac ttcctgttgg cgggatccgt cagtatggtc ctgcagcttc
    10021 tgccgttatt ctgccacaac tgaccagtca gaatgtcacg tttgataatg tgcagaatgc
    10081 cgtaggcgca gatttgcaga ttcgtttatt tggtaagccg gaaattgatg gcagccgtcg
    10141 tctgggggtg gtactggcta cttcagagag tgttgttgac gccattgaac gcgcgaagca
    10201 cgccgccgga caggtaaaag tacagggtta aacccggcaa aaaacgctac aaaaatgccc
    10261 gatcctagat cgggcatttt gacttttaca gcttagcgcc ttctacagct tcacgcgcca
    10321 gcttagtaat gcggtcgtaa tcgcccgctt ccagcgcatc tgccggaacc agccaggaac
    10381 caccgatgca cagcacgctt ttcagtgcca ggtagtcacg gtagttagcc ggagaaatac
    10441 cacccgtcgg gcagaaacgg acctgggaga acggacccgc gatcgcctgc agggctttca
    10501 cgccgccgtt agcttcagcc gggaagaatt tgaactcttt caaaccgtag tccataccca
    10561 gcatcagttc ggaaacagtg ctgatccccg gaatcagagg aatagtccct tcggtagcag
    10621 ctttcagcag cggctcggtc agacccgggc taattgcgaa ctgtgcaccc gcttcagtga
    10681 cttctgccag ctgctgtgga ttcagcaccg taccggcacc cacaatcgct tcaggcactt
    10741 ctttggcgat agcacggata gcgtcaactg cacactcggt acgcagagtc acttccagaa
    10801 cgcgcacccc accagcaacc aacgcttttg ccatcggcac cgcgtgttcc agttttttta
    10861 ccacgataac cggtacaacc gggccggtgg tcaggattga ttctgcactt gttttccagt
    10921 ttttcatcag agttttctct cgcctgatta caaatttgtc gtcttaaaaa gtgatacagg
    10981 ttgcgccctg ttcggcaccg gacagttttt cacgcaaggc gctgaataat tcacgtcctg
    11041 ttctcacgcg tgacgcgctc aggtcaggaa tgtgcggttc gcgagcagcc agttccgctt
    11101 cgtctaccag cagtgtcagt tcgcctgtct gtccattcac acgaatgatg tccccgtcgc
    11161 gcacttttgc cagcagcccg ccatcgtagg cttctggtgt tacgtggata gctgacggca
    11221 ctttacctga agcgccggag agtcgtccat cggtaactaa cgcaattttg aaacaccggt
    11281 ccaataatac accaagtggc ggcatgagtt tatgtaattc tggcattccg ttcgcttttg
    11341 gcccctgatg acggacaaca acgacacaat cgcggtccag caaacccgct tcaaaggccg
    11401 gcataacgtc atgctggctt tcaaaaacaa ccgctggcgc ttcaatcacc tggttctcaa
    11461 ccggcacggc agaggttttc ataaccgcac ggcccaggtt accgcttaac acttttgtcc
    11521 caccatgatg agagaaaggt tgttcgaagg aagcgatcac attgctgtcg agtgattttt
    11581 ccgccccttc ccgccagtcc agttcaccat tattcagcca tggttcaagg gtataacgaa
    11641 acagaccaaa gcctgccacc gtattgacat cttcatgcag caggcctgct ttgagcagtt
    11701 cacgcaccag aaccggtacg ccacctgccg cctggaagtg gttaatatcg gccggaccgt
    11761 tcgggtagag acgtgccatc agcggtacaa catcagaaag gtcagagaag tcatcccagt
    11821 taatctgaat accggccgcg cgcgccatcg ccaccaggtg catggtgtgg ttagtggaac
    11881 caccggtcgc caacagtgca acgataccgt tcaccaccac tttctcatcg atcatcttac
    11941 cgatcggcat ccattcatta ccattaccgg tcatgcgtgt aacctgacgc gcagcggcag
    12001 cggtcaaagc atcgcgcagc ggagaatccg gatgaacaaa agaagagcct ggcaactgca
    12061 tccccataaa ttccaccacc atctggttgg tgttggcagt accgtagaaa gtacatgttc
    12121 ccggcgcatg gtaagacgcg gcttctgact ccagtaaggc catgcggtcc actttacctt
    12181 cggcataaag ctggcgaata cgcacttttt ctttatttgg caaaccgctt gccatcggtc
    12241 cagacggcac aaacaccgca ggcaaatgac caaacgacag ggctgccatc gtcagacccg
    12301 ggacaatctt gtcgcacaca ccgaggaaca gagcaccatc aaacatgtta tgggacagcc
    12361 ccaccgccgc agacatcgct atcacttcgc ggcttagcag cgacaattcc attccatcct
    12421 gcccctgggt gacaccatca cacatcgccg gaacaccgcc cgcaacctga ccaaccgcat
    12481 tcgcttcatg cagggcttta cgaatgattt ctggatagtg ttcataaggc tggtgcgcgg
    12541 agagcatgtc gttataggag gtgatgatgg cgatattgtt acgcaacatg cttttcaaag
    12601 aggctttgtc ttctggctgg caggcagcga aaccgtgtgc caggttaccg catgccaact
    12661 gcgaacgatg aacggtcgac gttttcgctt gttctatccg ggcgagataa gcagagcgag
    12721 tctcgcgcga acgttcaatg attcgatttg ttacgcgtaa caattgtgga ttcataaagg
    12781 ctcctgaaat tgagttgtca gagcaggatg attcacaacg cgtttcattc agaggattta
    12841 tgagtgaaac gcctgtaacc ggagctcata gggcaaaaac gtttcagtca gtgtaataaa
    12901 aaaagcctcg tgggtgaatc cgcacgaggc ctgaaagtgt aaaaattgtt ctacaatctg
    12961 tgcaagatta tgttaccggt aaaataacca taaaggataa gcgctgatat tactcaaact
    13021 cattccagga acgaccatca cgggtaatca tcgccaccga ggcaacgggt ccccaggttc
    13081 cggcctgata cggtttcggc gcatcattgt ccatcgccca cgcctcagta atggagtcta
    13141 cccatttcca ggcttcttcc acttcgtcgc gacgtacaaa cagtgcctga ataccacgca
    13201 tggtttccag cagcaaacgt tcataggcat ccgccagatg cgtctgatta aaggtttctg
    13261 aatagctcag atccagcttg gtgatttgca ggttatgttt gtggtcaagg ccaggaactt
    13321 tattcagtac ctggatatcc acgccttcat caggttgcag acggatagtc agtttattct
    13381 gcggcagatc ctgccacgac tctttaaaca gattcagttc aggtgttttg aaatagacca
    13441 cgacttcaga acatttggtc ggcagacgtt taccagtacg caggtagaat ggcacaccgg
    13501 cccagcgcca gttatcaatg tcgacgcgga tcgccacgaa ggtttctgta ttgctacttt
    13561 tgttcgcgcc ctcttcttcc agatatcccg gcactttttt gccctgggcg aagcccgcag
    13621 tatattgccc gcgtacggtt ttttcgcgta cgttggagcg gtcgatgcgg cgcagggact
    13681 tcagtacttt cactttttca tcgcggatgc tgtctgcgct caggtcagat ggcggagaca
    13741 tcgcaatcat gcagagaatt tgtagcaggt ggttctgaat catgtcacgc atctgaccgg
    13801 ctttatcaaa atagccccag cgcccttcga tccccacttc ttctgccacg gtaatctcaa
    13861 catgatcaat ggtgcgattg tcccagttat tcacaaacag ggagttagca aaacgcagcg
    13921 ccaacaggtt cagcaccgtt tctttaccaa gatagtggtc gatacggtaa acctggcact
    13981 cctcgaagta ttcgccaacc tgatcattga tttcctgcga ggtcgccagc gacgtcccca
    14041 gcggtttctc catgactacg cgtgccggtt tagcattcag ttttgcctcg ccaagccctt
    14101 tgcaaattgc gccaaaagtg ctgggcggca tggcaaagta gttaatagtg atacgatttt
    14161 tttgatccag catcgcgccg agacggttga atgcagcagt gtcattgaca tcgagattac
    14221 aaaaatccag acgtgcactc agggtgtccc ataaaccttc atcaatggtt tctttcatga
    14281 aagtttcgag cgcctcgcgg acaactttgg tatatgccgc tttatcccag tcagcacgcc
    14341 ctacgccgat aatccgggtg tccgggttga gctgaccggc tttttccagt tgatacaggg
    14401 aaggcagcaa tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc aggtcacagg
    14461 cctgggctgt ttgcgttacc gccatgtcat tctccttaag ttaactaacc cggtacttaa
    14521 gccagggtat acttgtaatt ttcttacggt gcactgtact gcttttccga gcttgcgaaa
    14581 actgtaaacg cttatccacc cgtgcgatta cgggaaaagc gcgcaaagtg cggcaaaact
    14641 gataaaaaaa tcatcgtttt ccattcggta aaaatctgac actgatcatg ttatgaaaaa
    14701 aaataacaac ttttttatct gcttttgtca ttaacacggc acacaggcgt aatatctgac
    14761 aaaactgcat ttcgatttct ttcagtgcgg aaatcgtcat tacccgtgag tctctttaca
    14821 tcatgaatat gctggaaaaa atccagtctc agctggaaca tttgagcaaa tcagagcgca
    14881 aagttgccga ggtcattctg gcttcgcccg ataacgcgat ccattcgagt attgctgctc
    14941 tggcgctgga agccaatgtt agcgaaccga cggtgaatcg tttctgtcgc agcatggaca
    15001 cgcgcggttt tcctgatttt aaacttcatc tggcacaaag tctggcgaat ggcactccct
    15061 atgttaatcg caacgtcaat gaagatgaca gcgttgaatc atacacgggg aaaatatttg
    15121 agtccgcaat ggcaacgctc gatcatgtcc gtcattcact ggataaatct gccatcaacc
    15181 gcgccgtcga cttgctcact caggcaaaaa aaatcgcctt tttcggatta ggctcttctg
    15241 ccgccgttgc ccacgatgcg atgaataagt tctttcgttt taatgttccg gtggtgtact
    15301 ccgatgatat cgtgctgcaa cgcatgagtt gtatgaattg tagcgacgga gacgtggtgg
    15361 tgctgatttc tcacacagga agaacaaaaa atctggtcga gctggcgcag ctggcacgcg
    15421 aaaacgacgc catggtgatt gccctcacct ctgcgggtac cccgctcgcc cgggaagcaa
    15481 cgctggcaat taccctcgac gtaccggaag atactgacat ttatatgccc atggtttctc
    15541 gacttgcaca gctgaccgtg atagatgtgc tggcgacagg atttactttg cgacgcggtg
    15601 caaaattcag agataacttg aagcgggtca aggaagcgct gaaggaatcg cgttttgata
    15661 agcagttact taatttaagt gacgatcgct aaaagcgact gtcactgtcc taatcttata
    15721 cgacatccga atgagattaa tttatcgcca tcgcggcgtt atttcattcg gatttcatgt
    15781 tcaagcaaca cctggttgtt tcagtcaacg gagtattaca tgtccaaaag gcttcgcaga
    15841 acaaaaatcg ttaccacgtt aggcccagca acagatcgcg ataataatct tgaaaaagtt
    15901 atcgcggcgg gtgccaacgt tgtacgtatg aacttttctc acggctcgcc tgaagatcac
    15961 aaaatgcacg cggataaagt tcgtgagatt gccgcaaaac tggggcgtca tgtggctatt
    16021 ctgggtgacc tccaggggcc caaaatccgt gtatccacct ttaaagaagg caaagttttc
    16081 ctcaatattg gggataaatt cctgctcgac gccaacctag gtaaaggtga aggcgacaaa
    16141 gaaaaagtcg gtatcgacta caaaggcctg cctgctgacg tcgtgcctgg tgacatcctg
    16201 ctgctggacg atggtcgcgt ccagttaaaa gtactggaag ttcagggcat gaaagtgttc
    16261 accgaagtca ccgtcggtgg tcccctctcc aacaataaag gtatcaacaa acttggcggc
    16321 ggtttgtcgg ctgaagcgct gaccgaaaaa gacaaagcag acattaagac tgcggcgttg
    16381 attggcgtag attacctggc tgtctccttc ccacgctgtg gcgaagatct gaactatgcc
    16441 cgtcgcctgg cacgcgatgc aggatgtgat gcgaaaattg ttgccaaggt tgaacgtgcg
    16501 gaagccgttt gcagccagga tgcaatggat gacatcatcc tcgcctctga cgtggtaatg
    16561 gttgcacgtg gcgacctcgg tgtggaaatt ggcgacccgg aactggtcgg cattcagaaa
    16621 gcgttgatcc gtcgtgcgcg tcagctaaac cgagcggtaa tcacggcgac ccagatgatg
    16681 gagtcaatga ttactaaccc gatgccgacg cgtgcagaag tcatggacgt agcaaacgcc
    16741 gttctggatg gtactgacgc tgtgatgctg tctgcagaaa ctgccgctgg gcagtatccg
    16801 tcagaaactg ttgcagccat ggcgcgcgtt tgcctgggtg cggaaaaaat cccgagcatc
    16861 aacgtttcta aacaccgtct ggacgttcag ttcgacaatg tggaagaagc tattgccatg
    16921 tcggcaatgt acgcagctaa ccacctgaag ggcgttacgg cgatcattac catgaccgaa
    16981 tcgggtcgta ccgcgctgat gacctcccgt atcagctctg gtctaccaat tttcgccatg
    17041 tcgcgccatg aacgtacgct gaacctgact gctctctatc gtggcgttac gccggtgcac
    17101 tttgatagcg ctaatgacgg cgtagcagct gccagcgaag cggttaatct gctgcgcgat
    17161 aaaggttact tgatgtctgg tgacctagtg attgtcaccc agggcgacgt gatgagtacc
    17221 gtgggttcta ctaataccac gcgtatttta acggtagagt aagtacgttg ccggatgcgg
    17281 cgaaaacgcc acatccggcc tatagttcaa ttatagttca acagatttcg aatattctga
    17341 agcaaacttg aacttatcat caggcgaagg cctctcctcg cgagaggctt ttttatttga
    17401 tgggataaag atctttgcgc ttatacggct ggatttcgcc cggtttgcga gttttcagca
    17461 attttagtat ccaggtgtat tgttctggtc gcggaccaac aaaaatctcg acttcttcat
    17521 tcatccgccg ctcaatcgta tgatcatccg cctctaacag atcatccatc ggtgggcgca
    17581 cctgaatcgt cagacgatgc gtcttgccat cataaatcgg aaacagcggt acaacgcgcg
    17641 cacggcatac tttcatcaaa cgaccaatcg cgggcaacgt cgctttatag gtggcaaaga
    17701 aatccacaaa ttcgctgtgc tctgggccat gatcctgatc gggtaaataa tatccccagt
    17761 acccctgacg taccgactgg atgaatggtt taataccgtc atttctcgca tgcagacgac
    17821 cgccaaagcg acgacgcacc gtgttccaga cataatcaaa aaccgggttg ccctgattat
    17881 ggaacatcgc tgccattttc tgcccttgcg aggccatcag catggcagga atatcgacgg
    17941 cccaaccgtg cggcaccaga aagataactt tctcgttatt acgccgcatc tcttcgatga
    18001 tctccagccc ttgccagcca acgcgcggct gaattttctc cggcccgcgt attgccaact
    18061 cagccatcat tgccatcgct tgcggcgcgg tggcaaacat ctcatcaaca atcgcttcgc
    18121 gttcagcttc actacgttct ggaaagcaga gcgacagatt gattaacgca cgacggcgtg
    18181 agctttttcc cagtcgtccg gcaaaacgtc ccagccgtgc cagaatggga tcacggaact
    18241 ttggcggcgt taaagcgata cccgccatcg ctgctacacc cagccatgct ccccagtagc
    18301 gcgggtggcg aaaggattta tcaaactcag gaatgtattc gctattattt tttttcgttt
    18361 ccatgctttt ccagtttcgg ataaggcaaa aatcaatctg gtgatagtgt agcggcgcaa
    18421 cttgccccgc accaaataaa aaaagccggt actgactgcg taccggctgc gaatggatgt
    18481 taattaatca aacagtagct gcggcacaat ctctttggcc tgcgccagga attcgcgacg
    18541 atcggagccg gtcagccctt cggtacgcgg cagttttgcc gtcagcgggt ttacggcctg
    18601 ctggtttatc catacttcat agtgcagatg cggcccagtt gaacgtccgg tattaccgga
    18661 aagcgcgata cggtcgccac gtttcacctt ctgtcccggt ttcaccagaa tcttgcgcaa
    18721 gtgcatataa cgcgtggtgt agctgcgacc atgacgaata gccacataat aacctgctgc
    18781 gccactgcgt ttggcaacca ccacttcacc gtcacccact gaaagcactg gcgtaccttg
    18841 tggcatggcg aaatcaacac ctctgtgtgg tgcaacgcga cctgtcaccg gattagttcg
    18901 acgcgggtta aagttagacg agatacggaa ctgtttcgcc gtcgggaatc gcaagaatcc
    18961 tttcgccaga ccagtaccgt tacgatcgta gaatttgcca tcctcagcgc ggattgcgta
    19021 ataatcttta ccttctgaac gcaaacgtac gcccagcagc tggctttgct cacgtttacc
    19081 atcaagcatt tctcgagaca ttaataccgc aaattcatcg ccttttttca gtttgcggaa
    19141 atccatttgc cactgcatgg ctttaatcac tgcgctcact tcggcgctgg ttaaaccggc
    19201 gtttctggcg ctggcaacaa agctccctcc gacggtacct ttcagcagat tgttaaccca
    19261 ctctccttgc tgcatttcgc tggtcatttt aaaaccgtta gcggcagtac ggtcataggt
    19321 tcgggtttca cgacgagaca cttcccaggt gaggcgctgc agttcgccgt ccgcggttaa
    19381 tgtccaggag agttgttgac cgattttcag gttacgcaat tctttgtcgg cagcagcgag
    19441 ttgggtgata tcacccatat caataccata ctgattgaga atgctgctta gcgtatcgcc
    19501 agtggaaaca acatattcat gcacgcccgc ttcaccggcg attttgtcat ccagttcgtc
    19561 ctggggaatg gcttcatctt cttgtgcagc ttgatcaatc ggctcactgg cttcaggtaa
    19621 gagcgaacga atttcgttct gttccagctc aatggttttg acaattggcg tggcatcacg
    19681 gtgataaaca tagggccgcc agacagcgac ggccagagta agaacggtga gcgaccccaa
    19741 cataacgcgg tgtggtcgcg gtaaattatt aaacgccagg gcgacagagc gggctatctg
    19801 ttgcacgtaa tcacttcctc attaatctcc tttcaggcag ctcgcatact ggttggctaa
    19861 ttgactcagg aattctgaat agcttgtttt acccagtttg atattcgtcc ccaggggatc
    19921 caacgttccc atacgaacgg atgtccctcg tgcgacgctc tcaacgaccg ctggcctgaa
    19981 ctgtggctca gcaaaaacgc aggttgcttt ttgctcaacc aactgtgttc ttatttcatg
    20041 taaacgctgc gcgccaggtt gaatctcagg gttaacggta aaatgaccaa gcggtgtcag
    20101 tccgaactgt ttttcgaaat agccgtaagc atcgtgaaaa acgaaataac ctttcccctt
    20161 gagcggcgcg agctcgttac caacctgcgt ttcggttgag gccaattgtg cctcaaaatc
    20221 cttcaggttg gcgtcaagtt tggctcgact ttgcggcata agttccacta attttccatg
    20281 gattgcaacc gctgtagccc gcgctatctc tggggaaagc caaagatgca tgttgaaatc
    20341 gccgtgatgg tgatcttcgt cacttttttc cgcgtggtcg tgatcatcat catcgccgtg
    20401 aatacttttc atcagtagcg gtttcacatc ttcaagctgc gcaatcgtta cctgcttcgc
    20461 ttctggtaat ttgcttaccg gtttttgcat aaacgcttcc atctccgggc caacccaaac
    20521 gactaagtcc gcgttctgta agcgttttac atccgatgga cgcagtgaat aatcatgttc
    20581 tgaagcgccg tcaggcagta aaacctccgt ttctgttaca ccatcagcaa tggcagaagc
    20641 gatgaaccca acgggtttaa gcgaagcgac aacggcggca tctgcggcct gtgttgcacc
    20701 tccccagaga gcggcggata atgctgcgaa aagaagcgtt tttttatgta acataatgcg
    20761 accaatcatc gtaatgaata tgagaagtgt gatattataa catttcatga ctactgcaag
    20821 actaaaatta acatgacaag tctggtttcc ctggaaaatg tctcggtttc ttttggccaa
    20881 cgccgcgtcc tctctgatgt gtcgctggaa cttaaacctg gaaaaatttt gactttactt
    20941 gggccaaatg gcgcaggtaa gtcgacactg gtacgggtag tgctcgggct ggtaacaccc
    21001 gatgaagggg ttatcaagcg caacggaaaa ctgcgcatcg gctatgtacc gcagaagctg
    21061 tatctcgaca ccacgttgcc actgaccgta aaccgttttt tacgcttacg ccctggtaca
    21121 cataaagaag atattttgcc tgcactgaaa cgtgtccagg ccgggcacct gattaacgca
    21181 ccgatgcaaa agctctctgg tggcgaaacg cagcgtgtac tattagcgcg agcattgtta
    21241 aattgcccgc aattattagt gctggatgaa cccactcaag gcgtggatgt aaatggccaa
    21301 gtggcgttat atgaccttat tgaccagttg cgtcgtgaac tggattgtgg cgttttaatg
    21361 gtttctcacg atctgcatct ggtgatggca aaaaccgatg aagtgctgtg cctgaatcac
    21421 cacatttgtt gttccggcac accggaagtt gtttccctgc atccggagtt tatttcaatg
    21481 tttggtcctc gtggtgctga acaactgggt atctatcgcc atcatcataa tcatcgtcac
    21541 gatttacagg gacgaattgt tttgcgtcgg ggaaatgatc gctcatgatt gaattattat
    21601 ttcccggttg gttagccggg atcatgctcg cctgtgccgc gggtccgctg ggttcgtttg
    21661 tagtctggcg tcgtatgtct tatttcggtg atacgctggc tcatgcctca ttacttggtg
    21721 tcgcgtttgg tttgttgctg gacgtgaatc cattctatgc ggtgattgcc gttacgctgc
    21781 tgctggcggg cggtctggta tggctggaga agcgtccaca gctggcgatc gacacgttat
    21841 tagggattat ggcgcacagt gccctgtcgc tgggcctagt ggtcgttagt ctgatgtcta
    21901 acattcgtgt tgatttgatg gcttacctgt tcggtgattt actggcagtg acgccagaag
    21961 atctcatctc tattgcgatt ggcgtggtca tcgtggtggc tattttgttc tggcaatggc
    22021 gcaatttgct gtcgatgacg attagcccgg atctggcgtt tgttgatggt gtgaaattac
    22081 agcaagtgaa attgttgttg atgctggtga cggcattgac gattggtgta gcgatgaaat
    22141 ttgtcggtgc gttgattatt acttcgttgc tgattattcc tgcagctact gcgcgtcgct
    22201 ttgcccgtac gccggaacag atggctggtg tcgctgtttt ggtggggatg gtggcagtaa
    22261 ctggcggttt aaccttttcc gcgttttacg atacgccggc gggtccgtcg gtggtgctat
    22321 gtgcggcact gttatttatt atcagtatga tgaaaaagca ggccagctaa tctatcgctg
    22381 aacacatttg tcggatgcgg cgcgagcgcc ttatccgacc tacggttcgc tatctctggc
    22441 tggcctgata agacgcgaac agtgtcgcat cagacacact gccagtgtcg gatgcggcgc
    22501 gagcgaccaa tccgacttac ggcatttctg gcggcgttat gccaaagtga ttccacgccc
    22561 gcgtcgtcgc catacgcccg cgcggtgtac gctgcaaaaa gccttgctga atcaaataag
    22621 gttccagtac atcttcaatg gtttcacgtt cttcgccaat ggctgccgcc aggttatcca
    22681 gacctaccgg cccaccaaag aacttatcga ttaccgccag caacaatttg cggtccatat
    22741 aatcgaaacc ttcagcatcg acattcaaca tatccagcgc ctgagcagcg atatctgccg
    22801 agatggtgcc atcgtgcttc acttcggcga aatcacgcac tcgacgcagc agacggttgg
    22861 caatacgtgg cgtaccgcga gcgcgacgag ctacttcctg cgcaccgtca tcactcatct
    22921 caagccccat aaagcgtgca ctgcgactga cgatatattg cagatccggc acctgataaa
    22981 actccagacg ttgcacaata ccaaaacggt cgcgcaacgg tgatgtcagc gaacctgcgc
    23041 gcgtggttgc gccaatcagg gtaaacggcg gcaaatcaat tttaatggag cgtgccgccg
    23101 gaccttcacc aatcatgata tccagttggt agtcttccat tgccgggtac agcacttctt
    23161 caacaactgg cgataaacgg tggatctcat caataaacag cacgtcatgc ggttcaaggt
    23221 tagtgagcat cgcagccaaa tcgcccgcct tttccagcac cggaccagaa gtcgtgcgta
    23281 aattaacgcc catttcattg gcgacaatgt tggcaagcgt agttttaccc aaccccggag
    23341 gaccaaaaat caacaaatga tcgagggcat cgccgcgcag tttcgctgct ttgatgaaaa
    23401 tctccatctg tgaacgaacc tgcggctgac caacatactc ttccagtaat ttagggcgaa
    23461 tggcgcgatc tgctacatct tccggcaaag tggtaccggc agaaatcaga cggtctgctt
    23521 caatcatcct ttacctcata acgcggcgcg tagggcttcg cgaattaatg tttcactgct
    23581 ggcgtcaggg cgagcgattt tgctcaccat gcggcttgct tcttgtggtt tatagcctag
    23641 cgccaccagc gcggcaactg cttcttgttc agcatcgtcg gtcgccgggc tggcaggaga
    23701 cgtgagtacc aggtcggcgg ctggcgtaaa gagatcgcca tgcaaacctt taaatcggtc
    23761 tttcatttca acaatcaagc gttcggcggt ttttttgcca atacccggca gtttcaccag
    23821 tgcccccact tcttcacgct caacggcatt aacgaactgc tgcgctgaca ttccggagag
    23881 gatcgccagg gccaacttcg ggccgacgcc gttggttttg atcaactctt tgaacaacgt
    23941 gcgttcttgt ttattgttaa aaccgtacag cagttgcgcg tcttcacgca ccacaaagtg
    24001 ggtgaaaacg atcgcttcct gacccgcttc agggagttca taaaaacagg tcatcggcat
    24061 atgcacttca tagcctacgc cgcccacttc aattaacacc agcgggggtt gtttttcaat
    24121 gatgatgcct ctgagtctgc ctatcacatg acgctcctgc gtaatgaatc aaagataatg
    24181 ctgtatgata aaaaaatgct ggatagatat ccagcgtagg atgaagaaaa cttgcgaggt
    24241 gtcccgatga tctgaaaaat ggcacagtat aatttattca acaggttata ttggaagcaa
    24301 atatttgaat attacatatt cagtgaagaa atgcgtaata aaaatataca ttgcgactcc
    24361 tgaaaaaata atttttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa
    24421 cgtgaatatt aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg
    24481 tgctgcctta tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat
    24541 cattatcggt cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttca
//
LOCUS       DAJTXY010000050        24460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTXY010000050 DAJTXY010000000
VERSION     DAJTXY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.050"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..487)
                     /locus_tag="OO566_002136"
     CDS             complement(<1..487)
                     /locus_tag="OO566_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6605421.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LL"
     gene            760..948
                     /locus_tag="OO566_002137"
     CDS             760..948
                     /locus_tag="OO566_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6605422.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            960..1670
                     /gene="bcsQ"
                     /locus_tag="OO566_002138"
     CDS             960..1670
                     /gene="bcsQ"
                     /locus_tag="OO566_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6605423.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1667..4280
                     /gene="bcsA"
                     /locus_tag="OO566_002139"
                     /pseudo
     CDS             1667..4280
                     /gene="bcsA"
                     /locus_tag="OO566_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4291..6630
                     /gene="bcsB"
                     /locus_tag="OO566_002140"
     CDS             4291..6630
                     /gene="bcsB"
                     /locus_tag="OO566_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR6605424.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6637..7743
                     /gene="bcsZ"
                     /locus_tag="OO566_002141"
     CDS             6637..7743
                     /gene="bcsZ"
                     /locus_tag="OO566_002141"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6605425.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7802..7978
                     /locus_tag="OO566_002142"
                     /pseudo
     CDS             7802..7978
                     /locus_tag="OO566_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8306..10054
                     /gene="bcsC"
                     /locus_tag="OO566_002143"
                     /pseudo
     CDS             8306..10054
                     /gene="bcsC"
                     /locus_tag="OO566_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10136..12124
                     /gene="hmsP"
                     /locus_tag="OO566_002144"
     CDS             10136..12124
                     /gene="hmsP"
                     /locus_tag="OO566_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6605426.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12308..13594
                     /gene="dctA"
                     /locus_tag="OO566_002145"
     CDS             12308..13594
                     /gene="dctA"
                     /locus_tag="OO566_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6605427.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFSSGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13815..15311
                     /locus_tag="OO566_002146"
     CDS             13815..15311
                     /locus_tag="OO566_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6605428.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15407..16336)
                     /gene="kdgK"
                     /locus_tag="OO566_002147"
     CDS             complement(15407..16336)
                     /gene="kdgK"
                     /locus_tag="OO566_002147"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6605429.1"
                     /translation="MSKKIAVIGECMIELSEKDADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16568..17335
                     /gene="pdeH"
                     /locus_tag="OO566_002148"
     CDS             16568..17335
                     /gene="pdeH"
                     /locus_tag="OO566_002148"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6605430.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17405..19465
                     /locus_tag="OO566_002149"
     CDS             17405..19465
                     /locus_tag="OO566_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6605431.1"
                     /translation="MSKAGKITAAISGVFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19647..20969)
                     /locus_tag="OO566_002150"
     CDS             complement(19647..20969)
                     /locus_tag="OO566_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6605432.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21380..22393)
                     /gene="yhjD"
                     /locus_tag="OO566_002151"
     CDS             complement(21380..22393)
                     /gene="yhjD"
                     /locus_tag="OO566_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6605433.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22442..23341)
                     /locus_tag="OO566_002152"
     CDS             complement(22442..23341)
                     /locus_tag="OO566_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6605434.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23861..>24460
                     /locus_tag="OO566_002153"
     CDS             23861..>24460
                     /locus_tag="OO566_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6605435.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5787 a         6159 c         6733 g         5781 t
ORIGIN      
        1 caagcaacaa tgaaaattgt ttatcgttat tatttccggg attaattacc aaaagcgaac
       61 aatggtttaa cctgctccat ttattcattt tatccaacca tgagcgaagc cgctcggcag
      121 gaatgttttg ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta tggggatcaa
      181 tagaacactg caaatcacgg gccaaatagt atagaccttt ttcatgattt agcattgaaa
      241 ataattttat tttttccggc ccttgagaat catctaattg aaagattttc gccggatcgc
      301 tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt gtttgattcg
      361 ccagactgat agcatcttca tggcgatcga cgttaaacca ccagacgccg cctgctggca
      421 tatgtcgcag ctcatcccat aatgatgaga taccgataga gaatacaggg tccacaatgt
      481 ccctcatgtc accattttgt ctgattatca gtttactagc gaaagctaag aaataaacct
      541 accattgaaa ttaaaactta tcagatttag catgtgaatc aatttagaag gtcacaagac
      601 gtgtgatttt agtcttttcg tttttaactt tttctgttgc gtaacggaaa gtcaaaaagt
      661 gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg ttagaattgt
      721 atcatgaatg gtacggttat ttcataggga tcaagcaaaa tgaataacaa tgaaccagat
      781 actctgcctg atcccgcgat aggctatatc ttccagaatg atattgtggc gttaaagcag
      841 gcattttcac tgcctgatat tgattatgcc gatatttccc aacgcgaaca gttggccgcg
      901 gcattaaaac gctggccgtt gctggcagag tttgcgcaac aaaagtaggg gattggtgaa
      961 tggccgtact gggattgcag ggggtgcggg gaggcgtggg gacaacaacc atcaccgccg
     1021 cattagcctg gtcattacaa atgttgggag aaaatgtcct agtggtcgat gcctgcccgg
     1081 acaacttgtt gcgcctgtca tttaatgttg attttaccca ccgtcagggc tgggccagag
     1141 cgatgctgga tggccaggac tggcgtgacg ctgggttgcg ctacacctcg cagctcgatt
     1201 tgctgccttt tggtcagtta tccattgaag aacaagaaaa tccacagcac tggcaaaccc
     1261 ggctgagcga tatttgctcc ggcttacagc aactaaaagc cagcgggcgt taccagtgga
     1321 ttttaatcga cttaccgcgt gatgcctcgc agataaccca ccagctgctg agtttgtgcg
     1381 agcactcgct ggcaatcgtc aatgtggatg ccaactgcca tatccgactg catcagcaag
     1441 cgctgccgga tggcgcacat attttgatta atgacttccg tattggcagt caggttcagg
     1501 acgatattta ccagctttgg ttgcaaagcc agcgccgatt actgccgatg ctcattcatc
     1561 gtgatgaagc gatggctgaa tgcctggcgg ctgaagagat actgacgctg gcgaactggt
     1621 gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac
     1681 ccggtggttg cttatcccgc cggtcaacgc gcggcttatc gggcgttatc gcgattatcg
     1741 tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt ttctggatga tcctggcctg
     1801 gatttttatt ccgctggagc acccgcgctg gcaacgtatt cgcgcagaac ataaaaacct
     1861 gtatacgcca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     1921 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     1981 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     2041 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     2101 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     2161 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     2221 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     2281 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     2341 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     2401 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     2461 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     2521 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     2581 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     2641 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     2701 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     2761 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     2821 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     2881 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     2941 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     3001 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     3061 gtggctatac ctccgtgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     3121 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtacaa atcttccgtc
     3181 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     3241 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     3301 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     3361 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     3421 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     3481 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     3541 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     3601 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     3661 ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg
     3721 cggtatcggt agaaagcaaa caggtacgcc gatcgcaccg cgtggagatg acgatgcccg
     3781 cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     3841 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     3901 tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg
     3961 gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc
     4021 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     4081 agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     4141 cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     4201 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     4261 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca
     4321 gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     4381 ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg
     4441 caagtgatgt caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg
     4501 cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt
     4561 ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     4621 gcgatgctca acctcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta
     4681 aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt
     4741 aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg
     4801 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     4861 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     4921 aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc
     4981 ttgccgatgg tctttgcggg tgcgccggat gttgggctgc aacaagcatc tgccattgtc
     5041 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     5101 caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg
     5161 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     5221 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa
     5281 ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa
     5341 ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc
     5401 acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca
     5461 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     5521 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     5581 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     5641 cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct
     5701 attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac
     5761 ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg
     5821 gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat tccgatgccg
     5881 gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa
     5941 accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact
     6001 gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat
     6061 ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa
     6121 ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca
     6181 ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa
     6241 acccagtcaa cgctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg
     6301 tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg
     6361 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
     6421 atccgcgagt acggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg
     6481 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
     6541 gcggctatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt
     6601 cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggaatc
     6661 gtgacgatgc tgttgttggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg
     6721 gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg
     6781 cgcaaaatca ccacctccga agggcaaagt tacggtatgt tctttgccct ggcggctaac
     6841 gaccgtgcag ctttcgataa tattctcgac tggacgcaga acaatctcgc tcagggttct
     6901 ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg
     6961 ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg
     7021 gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcgttgct aaaacgtatc
     7081 gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg
     7141 ggttttgctg aggataacag ctggcgtttt aaccccagct acatgccgcc gacgctggcg
     7201 cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta
     7261 ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa
     7321 ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac
     7381 atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt
     7441 aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct
     7501 acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcctttttta
     7561 caaaaccgcg atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt tcccggcagc
     7621 gatacctatt acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc
     7681 cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac
     7741 taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa
     7801 ccatcagcag accctgcgga tggacagcgg caacactaat gccgtgcgcg ggctggcgaa
     7861 tatttaccgc cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
     7921 ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
     7981 ggcagaggta ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg
     8041 actggcgctg gaccccggca gcgtatggat tacttaccga ctttcgcagg atctctggca
     8101 ggccggacaa cgcagccagg ccgatacgtt aatgcgcaat ctggcgcagc agaagccgaa
     8161 caacccggag caggtttacg cttacgggct gtacctctca ggtcacaacc aggacagagc
     8221 ggcgctggcg catatcaaca gcctgccgcg cgcgcagtgg aacagcaata ttcaggagct
     8281 ggttaatcga ctgcaaagcg atcaggtgct ggaaaccgct aatcgcctgc gagaaagcgg
     8341 caaagaggct gaagcggaag cgatgctgcg tcagcaacca ccttccacgc gtattgacct
     8401 cacgctggct gactgggcgt aacaacgacg tgattacacc gccgcccgca ctgcatatca
     8461 gaatgtcctg acgcgggagc caactaacgc cgatgccatt cttggtctga cggaagtgga
     8521 tattgctgcc ggtgacaaag cggcggcacg tagccagctg gcgaaactgc ctgccaccga
     8581 taacacctcg ctgaacactc agcggcgcgt ggcgctggca caggcgcagc ttggagatac
     8641 cgcagcggcg cagcagacgt ttaataagtt gatcccgcag gcaaaatctc agccaccgtc
     8701 gatggaaagc gcgatggtgc tgcgcgacgg tgcgaagttt gaagcgcagg cgggcgatcc
     8761 aaagcaggcg ctggaaacct acaaagacgc catggtcgca tccggtgtga ctacgacgcg
     8821 tccgcaggat aacgacacct ttacccgact gacccgtaac gacgagaaag atgactggct
     8881 gaaacgcggc gtgcgcagcg atgcggcgga cctctatcgc cagcaggatc ttaacgtcac
     8941 ccttgagcac gattactggg gttcgagcgg caccggtggt tactccgatc tgaaagcgca
     9001 cactaccatg ttgcaggtgg atgcgccgta ttctgacggg cggatgttct ttcgcagtga
     9061 tttcgtcaat atgaacgtcg gcagtttctc cactaatgcc gatggcaaat gggatgacaa
     9121 ctggggcacc tgtacattac aggactgtag cggcaaccgc agccagtcgg attccggtgc
     9181 cagcgtggcg gtcggctggc gaaatgacgt ttggagctgg gatatcggta ccacgccgat
     9241 gggcttcaac gtggtggatg tggtcggcgg catcagttac agcgatgata tcgggccgct
     9301 gggttacacc gttaacgccc accgtcggcc catctccagt tctttgctgg cctttggtgg
     9361 gcaaaaagac tccccgagca ataccgggaa aaaatggggt ggcgtacgtg ccgacggtgt
     9421 ggggctaagt ctgagctacg ataaaggtga agtaaacggc gtctgggcat cgcttagtgg
     9481 cgaccagtta accggtaaaa atgtcgaaga taactggcgc gtgcgctgga tgacgggcta
     9541 ttactataag gtcattaacc agaacaatcg ccgcgtcaca atcggcctga acaacatgat
     9601 ctggcattac gacaaagatc tgagtggcta ctcactcggt cagggcggtt actacagtcc
     9661 gcaggaatac ctgtcgtttg ccataccggt gatgtggcgg gagcgcacgg aaaactggtc
     9721 gtgggagctg ggggcgtctg gctcgtggtc gcattcacgc accaaaacca tgccgcgtta
     9781 tccgctgatg aatctgatcc cgaccgactg gcaggaagaa gctgcgcggc aatccaacga
     9841 tggcggcagc agtcagggct tcggctacac ggcgcgggca ttacttgaac gacgtgttac
     9901 ttccaactgg tttgttggca cggcaattga tatccagcag gcgaaagatt acgcacccag
     9961 ccatttcctg ctctacgtac gttattccgc cgccggatgg cagggtgaca tggatttacc
    10021 gccgcagccg ctgatacctt acgccgactg gtaagttttc agatagcgcc tctcttaatg
    10081 ccgctgcgat cgggtatact cgggcggcaa tctgggattt ccggggggag acaatttgcg
    10141 cgtaagtcgc tcgttaacaa tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt
    10201 cgtttttatt ttttgcaccg ttttgctgtt ccatctggtc cagcagaatc gctataacac
    10261 ggctacgcaa ctggaaagca ttgctcgctc tgtccgcgaa cccttatctt cagctatttt
    10321 gaaaggcgat attcccgaag cggaagctat tcttgccagc attaaaccgg caggcgtggt
    10381 cagccgtgcc gatgtagtgc tgcctaacca gttccaggcg ctgcgtaaaa gttttattcc
    10441 agagcgtccg gtgccggtaa tggttactcg cctgtttgag ctaccggttc aaatctcgct
    10501 gggcgtctac tcgctcgaac gtccggcaaa tccgcagcca atagcctatc tggtgctaca
    10561 ggcggattcc ttccgtatgt ataagttcgt gatgagcact ctctcaacgt tagtgaccat
    10621 ttacttactt ttgtcgctta tcctgaccgt cgccatcagc tggtgcatta accgcctgat
    10681 tttgcatccg ttacgcaata ttgctcgcga acttaacgcc atcccagccc aggagcttgt
    10741 tggtcaccaa ctggcattac cgcgtctgca tcaggacgat gaaatcggta tgttggtgcg
    10801 cagttacaac ctcaaccagc aattgctgca gcgccattat gaagaacaga acgaaaatgc
    10861 gatgcgcttc ccggtgtcgg atttgccgaa caaagccttg ctgatggaga tgctggagca
    10921 ggttgtcgcg cgtaaacaaa ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga
    10981 tactgcgggc gtgctgaaag aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa
    11041 actcaaatcg gtactgtcgc cacgtatgat cctcgcgcag attagcggtt atgactttgc
    11101 tgtcattgcc aacggtgtac aggaaccgtg gcacgcaatc actttgggtc agcaagtact
    11161 cactatcatg agcgagcgcc tgccgattga acgtattcaa ctccgtccgc actgtagcat
    11221 tggcgtggcg atgttctacg gcgatctcac cgccgaacag ctttacagtc gcgctatttc
    11281 tgcggcattt accgctcgcc ataaaggcaa gaatcagatt cagttctttg atccgcagca
    11341 gatggaagca gcccagaagc ggttgacgga agagagcgat atccttaatg cactggaaaa
    11401 tcatcagttt gcaatttggt tacagccaca ggtcgagatg accagcggta aactggtcag
    11461 tgcggaagtg ttactgcgta tccagcaacc ggatggcagt tgggacctgc cggatggctt
    11521 aatcgatcgc attgagtgct gtgggctgat ggttaccgtc ggtcactggg tgctggaaga
    11581 gtcctgtcga ttgcttgcag cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa
    11641 cctctctgcg ctgcaactga tgcacccgaa tatggtggcg gatatgctgg aactgttaac
    11701 ccgctatcgc attcagccgg gaacactgat tctggaagtg acagaaagcc gacgtattga
    11761 cgaccctcat gctgcggtgg caatcctccg tccgctgcgc aatgccggag ttcgggtggc
    11821 gctggatgat ttcggcatgg gctacgcagg gctgcgtcag ctgcagcata tgaaatcgtt
    11881 gccaatcgac gtactgaaaa tcgacaaaat gtttgttgaa ggcttgccgg aagatagcag
    11941 catgattgct gcaattatca tgctggcgca gagcctgaac ttacaaatga ttgccgaagg
    12001 cgtggagact gaagcacaac gagactggct ggcaaaagcg ggcgttggta ttgcccaggg
    12061 cttccttttt gctcgcccac tccctattga aatcttcgaa gagagttacc tggaagaaaa
    12121 gtagctaccc caaactgatt acaaaacttt aaaaagtgct ggtttgtgcg agccagctca
    12181 aactttttaa cctttttgtt tcaattatga tccaggtaca tttctgtgat gttgtctggg
    12241 tgttatttta aggccgcagg tacccccata accttacaag acctgtggtt ttactaaagg
    12301 acaccctatg aaaacctctc tgtttaaaag cctttacttt caggtcctga cagcgatagc
    12361 cattggtatt ctccttggcc atttctatcc tgaaataggc gagcaaatga aaccgcttgg
    12421 cgacggcttc gttaagctca ttaagatgat catcgctcct gtcatctttt gtaccgtcgt
    12481 aacgggcatt gcgggcatgg aaagcatgaa ggcggtcggt cgtaccggcg cagtcgcact
    12541 gctttacttt gaaattgtca gtaccatcgc gctgattatt ggtcttatca tcgttaacgt
    12601 cgtgcagcct ggtgccggaa tgaacgtcga tccggcaacg cttgatgcga aagcggtagc
    12661 ggtttacgcc gatcaggcga aagaccaggg cattgtcgcc ttcattatgg atgtcatccc
    12721 ggcgagcgtc attggcgcat tttccagcgg taacattctg caggtgctgc tgtttgccgt
    12781 actgtttggt tttgcgctcc accgtctggg cagcaaaggc caactgattt ttaacgtgat
    12841 cgaaagtttc tcgcaggtca tcttcggcat catcaatatg atcatgcgtc tggcacctat
    12901 tggtgcgttc ggggcaatgg cgtttaccat cggtaaatac ggcgtcggca cactggtgca
    12961 actggggcag ctgattatct gtttctacat tacctgtatc ctgtttgtgg tgctggtatt
    13021 gggttcaatc gctaaagcga ctggtttcag tatcttcaaa tttatccgct acatccgtga
    13081 agaactgctg attgtactgg ggacttcatc ttccgagtcg gcgctgccgc gtatgctcga
    13141 caagatggag aaactcggct gccgtaaatc ggtggtgggg ctggtcatcc cgacaggcta
    13201 ctcgtttaac cttgatggca catcgatata cctgacaatg gcggcggtgt ttatcgccca
    13261 ggccactaac agtcagatgg atatcgtcca ccaaatcacg ctgttaatcg tgttgctgct
    13321 ttcttctaaa ggggcggcag gggtaacggg tagtggcttt atcgtgctgg cggcgacgct
    13381 ttctgcggtg ggccatttgc cggtagcggg tctggcgctg atcctcggta tcgaccgctt
    13441 tatgtcagaa gctcgtgcgc taactaacct ggtcggtaac ggcgtagcga ccattgtcgt
    13501 tgctaagtgg gtgaaagaac tggaccacaa aaaactggac gatgtgctga ataatcgtgc
    13561 gccggatggc aaaacgcacg aattatcctc ttaatctcac cacttatgcc cgcactccct
    13621 tccccgagtg cgggtgtttg cgcataattg acccctgtgc ctgaacttac cacctgggtg
    13681 agttcaggtt tatttcactt cttccgtgac attttcatgt tcttgcggtc taacacgaag
    13741 tgtttttaac gtcatttaga ctgaccgaca acgggcagtt tttttattac caggatagtt
    13801 gatcaggggt tcacatgcag ggcataaaaa ttcgactttt agcgggcggt ttgctgatga
    13861 tggccactgc tggctatgtg caggcagatg cgctccagcc tgatccagca tggcaacagg
    13921 ggacgctttc caacggttta cagtggcaag tgctgaccac cccccagcgt cccagcgatc
    13981 gtgttgaaat tcgcctgctg gttaataccg gttcgctcgc cgaaagtaca caacagagcg
    14041 gttacagtca cgccatccct cgtattgcgc taacgcaaag cggtggcctt gacgcagcac
    14101 aggcgcgttc attgtggcag caggggatcg accctaaacg cccgatgccg ccggtaattg
    14161 tctcttatga caccacgctg tttaatctga gtttgcccaa taaccgtaac gatttgctga
    14221 aagaagcgct ctcttatctg gcaaatgcca ctggcaaatt gaccatcaca ccagaaacca
    14281 tcaaccacgc gctgcaaagt caggacatgg tggcaacctg gcctgccgat actaaagagg
    14341 gctggtggcg ctatcgtctg aaagggtcaa ccttgttagg tcacgatcct gccgatccgc
    14401 tgaaacaacc cgttgaagcg gaaaaaatta aagatttcta tcagaaatgg tacaccccgg
    14461 atgcaatgac gctactggtg gtgggaaacg tggatgcgcg ctcggttgtc gaccaaatca
    14521 acaaaacgtt tggcgaactg aaaggcaaac gtgaaacgcc agctccggtg ccgacgcttt
    14581 ctccgctgcg tgcggaagcg gtgagtatta tgactgacgc ggtgcgtcag gaccggttat
    14641 ctatcatgtg ggatacgccg tggcagccga ttcgtgaatc agccgcactg ctgcgctact
    14701 ggcgtgcgga cctggcccgc gaggcgctgt tctggcatgt tcagcaagcg ttaagcgcca
    14761 gtgacagcaa agacatcggt cttggatttg actgccgtgt gctgtatctg cgtgcgcagt
    14821 gtgccatcaa catcgaatca ccaaacgaca agctgaacag caaccttaat ctggtggcgc
    14881 gtgaactggc gaaggttcgc gataaaggtc tgccggaaga agagttcaat gcgttagtgg
    14941 cgcaaaagaa actggagctg cagaaactgt ttgccgccta tgcacgggct gataccgata
    15001 ttctgatggg ccagcggatg cgttcgttgc aaaatcaggt cgtcgatatc gcgccggagc
    15061 agtatcagaa actgcggcag gatttcctta acagcctgac tgtggagatg ttaaatcagg
    15121 atctgcgtca gcagttgtcg aatgatatgg cgttaatact gctgcagccg aaaggcgagc
    15181 cggaatttaa catgaaagcg ttgcaggcgg cctgggatca aatcatggcc ccatctaccg
    15241 ccgctgcgac cacctctgtc gccacggatg acgtacatcc tgaagtgacg gatattccac
    15301 ctgcacagta atgcctgatg gtgatgagtt agcacaacca tcaggtattt atggatgagc
    15361 tggatagagg ggtaacactt tatccagcct tttgcatatg ctgcgtttac gctggcatcg
    15421 cctcacgcgg gataatcgcg ccgcgatact gaataacggt acttgcggtc aggtgcccgc
    15481 gtttcgccgc gttttccgca ctgccgcccg tcaaacgtac cgccagataa ccggcgctga
    15541 aagagtcgcc agctgcggtg gtatcgatca ctttttcttt cggcagtttc accgccggaa
    15601 catcaactaa cgcttcacca gcaatggaca ccaggcaaga atccgccccg cgtttcacca
    15661 ccacttcttt cacgcccgcg ttatgggtgc gcgcaatgac gtcttccacc ggctgttgac
    15721 cccacagcgc gtcttcgtcg tccagcgtca ggaaggcgat atccgtgcat tccagcattt
    15781 gctggtacac ctgctgtgtc tcttctttgc tggcccacag gcgcggacga tagttattgt
    15841 cgaaaatcac ttttccgcct ttggcgcggc attcgcgcag cagggaaagc agcttttcgc
    15901 ggctggtcgg gcttaagatc gccaggctaa tcccgctcag gtagagataa tcgaaattcg
    15961 ccagctcttc gcaaatcgcc gcagactgct cgctcgccag ccagaatttg gcggcggctt
    16021 cgttacgcca gtagtagaac gtacgttcgc cggtgctgtc ggtttcaatg tagtaaaggc
    16081 ccggaagacg gttttccatc cgttgggtca gggaagtatc aacgttctcg ccgtgccagg
    16141 cgtccagcat ctgctggcta aaactgtccg ttcccagcgc cgttacgtaa tgaacggtta
    16201 atgccgcagg atcgacctga cgggcgatat agacggaagt gttcagggta tcgccgccga
    16261 aaccgcgctt aacgtccgcg tctttctcgg aaagctcaat catgcattcg ccaatcacgg
    16321 caatcttttt ggacatagtc gtgaacctga tctgtaaaaa attagcgtta gtttgcgctg
    16381 tggtggtttg ctggtcaacc atattaaaac agtgttccat tatttttttg agccagaacg
    16441 tattcctgaa agattccgtt gtggagtgag gaaatttacc ccggccaggc ggtaaagttc
    16501 tgcccttacg cgccgataat ctttgtcgag tccgggcagc atcactttta aacacaggac
    16561 atctttgatg ataaggcagg ttatccagcg aataagcaac cctgaagcaa gcatcgagag
    16621 cttgcaggaa cggcgttttt ggttgcagtg tgagcgtgct tacacctggc agccgatcta
    16681 tcaaacatgc gggcggttaa tggccgtgga gctattaacg gtggtcacgc atcccttgaa
    16741 cccttcgcaa cgcctgccgc cggatcgcta ttttactgaa atcaccgtca gccatcggat
    16801 ggaggttgtg aaagagcaga ttgatttgct ggcgcaaaaa gccgacttct ttgtagagca
    16861 cggcctgctg gcatcggtca atattgatgg ccctacgctc atcgccctgc gtcagcaacc
    16921 aaaaatcctg cgccagattg agcgtcttcc ctggctgcgt ttcgaactgg tggagcatat
    16981 ccgtctgccg aaagattcaa cctttgcctc gatgtgtgaa tttggcccgc tgtggctgga
    17041 tgattttggt accgggatgg caaatttctc tgcactaagt gaagtgcgtt atgactacat
    17101 caaaatcgcg cgagaactgt ttgtgatgct gcgtcagtcg ccggaaggcc gcacactctt
    17161 ttctcagctt ttacatctaa tgaatcgcta ttgtcgcggg gtgattgtcg agggcgtaga
    17221 aacaccggaa gagtggcgtg atgttcagaa ctcgcccgca ttcgccgcac aaggctggtt
    17281 tctttcacgc ccggcaccga tggaaacgct gaatacggcg gttctggcgc tataagctgc
    17341 ctcattttcc gccgggctgg actatcttta ggactggcac aggaagaata tgaggaagcg
    17401 aacgatgagc aaggcaggca aaataaccgc tgcgatttca ggggttttct tgttgttgat
    17461 tgtcgtggcg atcattttga ttgcaacttt tgactggaat cgactcaaac cgaccatcaa
    17521 ccagaaagtc tctgcggagt tgaatcgtcc gttcgctatc cgtggcgatc tgggcgtggt
    17581 gtgggagcgg caaaaacaag aaactggctg gcgcagctgg gtgccgtggc ctcatgtaca
    17641 tgcagaagac atcattcttg gtaatccacc ggatattccc gaagtcacga tggtgcattt
    17701 gccgcgagta gaggcaacgc tggcaccgct ggcgctgctg accaaaacgg tctggctgcc
    17761 gtggatcaag ctcgaaaagc ccgacgcgcg cctgattcgc ctctctgaaa agaacaataa
    17821 ctggacgttt aatcttgcca acgatgacaa caaagacgcg aatgcaaagc cgtcggcatg
    17881 gtcgtttcgg ctggataata ttcttttcga tcaagggcgg atcgccattg atgacaaagt
    17941 aagcaaagcg gatctggaaa tttttgttga tccgttaggc aagccgctgc cattcagcga
    18001 agttactgga tcgaaaggta aagcggataa agaaaaggtg ggcgattaca tttttggcct
    18061 gaaggcgcag ggacgttata acggtgaacc gctcacgggt acgggaaaaa taggcggtat
    18121 gctggcgctg cgtggcgaag ggacgccgtt tccggtacag gctgatttcc gttcaggtaa
    18181 tacccgtgtt gcttttgatg gcgtcgtgaa tgacccaatg aagatgggcg gtgtcgattt
    18241 acggcttaaa ttttctggcg attcactggg tgatctctat gaactgacgg gcgttctgct
    18301 gcccgatacc ccgccgtttg aaacggatgg tcggctggta gcgaaaatcg acactgaaaa
    18361 atcgtcggtc tttgattatc gcggttttaa tgggcgaatt ggcgatagcg atatccacgg
    18421 ttctctggtc tacaccaccg gaaagccacg accaaaactg gaaggtgatg tcgagtcgcg
    18481 gcaattgcgg ctggcggacc tgggaccgtt gattggcgtt gattccggga aaggggcaga
    18541 aaagtcgaaa cggtctgaac agaagaaggg cgaaaaaagc gttcagcctg cgggcaaagt
    18601 gctgccttat gaccgcttcg aaaccgataa atgggacgtt atggatgccg atgttcgctt
    18661 caaagggcgg cgcattgagc atggcagtag cctgccgatt agcgatcttt ctactcatat
    18721 catcctcaaa aatgctgacc tgcgcctgca accgctgaaa tttggcatgg cgggcggcag
    18781 cattgcggcg aatattcatc tggaaggcga taaaaagccg atgcaggggc gggcagatat
    18841 tcaggctcgt cgactgaaac tgaaagaact gatgcccgat gtggaactga tgcagaagac
    18901 gctgggggaa atgaacggtg acgcggaact acgcggtagc ggtaactcgg tggcggcgct
    18961 tttaggcaac agtaacggca acctgaaact gttgatgaat gacgggctgg tgagccgcaa
    19021 cctgatggag attgttgggc tgaatgtcgg caactacatt gtcggtgcga tatttggtga
    19081 cgatgaggtg cgggtgaact gcgcggcggc gaatctgcat attgccaacg gcgtggcgcg
    19141 cccgcagatt tttgctttcg atactgagaa cgcgttgatt aatgttaccg gcacggcaag
    19201 ttttgcttcg gaacagctgg atttgactat tgatccggag agtaaaggga ttcggattat
    19261 cacactgcgt tcgccgctgt atgtgcgtgg gacgtttaaa aatccgcagg ctggggtgaa
    19321 agccggaccg ctgattgccc gtggtgctgt tgctgcggca ctggcaacgc tggtaacacc
    19381 ggcggcggcg ttactggcac tgatctcacc ttccgaaggg gaggctaatc agtgtcggac
    19441 gatattgtcg cagatgaaga agtgatgata attgccggat gtctgacatc cggcaattaa
    19501 acagatttag atttttggca aagtgtgctt tgttcatgcc ggatgcggcg tgaacgcctt
    19561 atccggccta caaatccttg tgaattcaat atattgtatg gaaattcagt cctgataagc
    19621 gtagcgcatc aggcttttca cacttattac aacgactgat gtcgcgtctc atgggtcagc
    19681 agcagggcga ttaacgtcaa gccagccatc gccgccagat ataaccccac cgcacctaac
    19741 ccgtagttag cctgcaacca agctgcgata tacggcgcaa cggaagcccc gagaatcgac
    19801 gatacgttgt aagagaacga tgctccggtg taacgcactt ctgtcggaaa cagctctggt
    19861 aacagcgcac ccatcggccc gaaggtcagc cccatcagac ttaaccccag cagcaggaag
    19921 gcaaaaacca gaatcgggtt gccagaaccg agcagcgggt taaaggcgaa cagcgcgaac
    19981 aggatgatca gcgtggtgat gatcaccatg cttttacggc gaccaaaggc atcagccagt
    20041 aatccggcga ctggcaccat cacgccaaaa ccaataactg ccatcatcag catccacaac
    20101 acttcgttac gcggcaggcc aagcccaact ggcgcggcgg cggtactgaa ggtcatagag
    20161 tagaccgtca tgatgtaaaa cagcgtatag gttgccagca taatgaacgt acccagtacg
    20221 gttacgcgaa catgtttggt cagcagcgta cccagcggga tcttcacctg ttttttcgct
    20281 ttagcgactt tctcaaacac cggcgactca tgcagcgaca cgcgaacata caggccgata
    20341 atgaccagca ccgccgagaa gataaatggc acacgccagc cccagctcat aaactgctca
    20401 tcggtcagta gccaggaaag cagcaagaaa gtgccgttgg caaagaagaa gccaatcggt
    20461 gcgcctagct gtgggaagga gccatacagt gcacgtttgc gcggtggggc gttttcagtc
    20521 gccagcagcg ccgcgccgcc ccattcaccg cctaagccca gaccctgacc aaatcgagcc
    20581 agcgccagca gcagtggggc gaaaatacca atcgtggcat agcccggcag cagaccaatc
    20641 accacggtcg aaatccccat cgttagcaac gaggcgacca gcgtcgcttt acgcccaacg
    20701 cgatcgccaa aatgaccaaa aacggcagag ccaatggggc gcgcgacgaa ggcgatggcg
    20761 aaggtggcga gcgactgtag cgttgctgcc gttggatcgc cctgcgggaa gaagatatgc
    20821 ggaaacacaa taacggccgc agtggcgtaa atgtaaaagt cgaagaactc aatggccgtg
    20881 ccaatgagag aggcgacaag gactttatta cgcgagttga tcggcgtgta ttcttgttcg
    20941 tggtcgagtg ttgtggctgt tgcttgcata atcttttctt attttttggc taacgaatag
    21001 ccatattacg cacagcaaaa gtgatatttc aatgtgtaac aaatcgcgga aatggtcaaa
    21061 aaccaggcaa aaaggggata atgtttaggc cagtgatttt tcttctatga aatgcttcac
    21121 ataattttat aaatagtgga taaaactggt ttcaggttaa ataaaagtta ccaactggat
    21181 ttatatgctg gggttatgag ttggagtgaa attgtttgct cccaaataga tggctgaaga
    21241 ggctctgggg aagagtttca agaggttatg tgcgcattat agccggatgc gatgctggcg
    21301 catcttatcc ggcctacggg gagccaggcc gaataaaatc gtatgccgga taaggcgttt
    21361 acgccgcatc cggcctattt taaggctgcg ttttccctgg cattcgcggg tcgtctttat
    21421 attcggcggt ggcaatccac gccgcgcaaa acagcgtcaa acgggcgaag aagtagaaaa
    21481 atgccatcag ccccagcacg gaaccaaatg ctgcgccaga gggggatttc atcagcgatg
    21541 gcagggtgta ggtcatcacg attttaatca cttcaaaacc aatagcagcc agaaatgttc
    21601 cgcgaatcag ggcttttttg cgcggacggt ggcgcggcaa ccgccagaag atccagaaga
    21661 aaagcagata gttggcgaag atggaaatcg ccaggccaat caatcgccac gtcggtttca
    21721 gccactcaat gctgttcagg tgcagggcgc taataatcat ttgctgcgcc gaaccggcaa
    21781 ccgaggtgat cgaaagcgtc acaatcagcg caatcaacaa accaatcagt gaaataaaat
    21841 cacgcagata ttttacccag aacttctcct gatcttgcgg cgagcgttcc cagacatcgc
    21901 gcgactgggc acgaatcgct tcacgcaggt tacccatcca gttgatgccg gaataaagtg
    21961 ccaccgccag gccgacaagc cctacagtcg tacgctgctg aacggcggtg ttgatggtgt
    22021 ttttcaacgt ggcggctagt gtcggatcgc tgatgttttg cagaattttg tcgaagatat
    22081 cctgtagcaa catcggatgg gaggccagca caaagccccc tgcggcaaac gacaccatca
    22141 aaatcgggat catcgacaaa aatgagaaat aggtgatagc cgcaccaaac tggttaccca
    22201 gccgatcgtt aaagcgttct gtcgcgcgaa taaggtgcgc aataaccggt tggcgttgga
    22261 ctttttcggc agtggtggtg acggtttcca gcgcctggct aactttgccg cctttctcgc
    22321 tattatccaa ctgcttaatc ggctcgtgct ccagatcctg ggtgggacgt ttgatctcgt
    22381 tttcctgcgt catccgctct tttgtccttc tgtcgtggtc attcttaaaa agtatagtcg
    22441 gtcagtccag gtgctctttc attacgccag ccagccactg cataaacagg tttacacgcc
    22501 gggaaagctc ccgacgctgc gggtaaacca gcgaaaggga gagcggctcg gcacggtagc
    22561 ctggcaacac ttcaataagc cgcccggcac gcagggcttc gcgcacggca atgcgcggga
    22621 tctgaataat ccccagaccg gcaagaccgg cggcgagata gttttcgcta ctgtttaccg
    22681 tcaacatgcc gccagactta aaccactgga cgccattaac gctggcaacc tcaaaaccta
    22741 acggatgtac acccaggtgc ggtgtataac gcactattgc gtgtgaagtc agatcatcgg
    22801 ggctttgcgg ataaccaaag cgcgtcagat agtgcggact ggcacagttg accatggtta
    22861 gtttgccgag gggacgggcg ataacgccat cttccggtaa cgcgccagtg cgtatcacgc
    22921 aatcaaaacc atcatgaaga atatctaccg gacggttatg gctactcagt tccagctcaa
    22981 ttcccggatg ctgatagaga aattccgaca gacgcggcag taacaggttt ttcgcgattc
    23041 ctgacgggat gtcgatgcgt aatttaccgc tgatactggt ggcatcctgt tgaaacagac
    23101 cgtccagttc gctgagatta ctcaacacat cttttgcccg ttgataatag gtcatgcctt
    23161 ctggcgtgag tttgacccgt cgcgtagtgc gctggagaag ctgggtgcca agttgatgtt
    23221 ccagtgcctg tatctggcgc gaaacacttc cctttggcaa agcaaagaaa tccgccgcgc
    23281 gggaaaaact ttccagctcc gcgactttga tgaacaactg cattgcgtga attttatcca
    23341 tttctttgac cgattgttgt tcacagcgaa acagtaacgc gcatatcgca ttgtttattg
    23401 ttttttctaa catctaatgc cgggtttcac aatgttgtca tgacgaatgt ggcgatttct
    23461 cagcaggaaa agggcggaat agctttatga gaaaggtgaa tccccttaaa cagagcgtat
    23521 ttttcagtga ttccctttaa agtgaataga ggacgtattg gctatttgtg cttatttttg
    23581 tctcattgcc atttgttatt ttggcttgta gtcgcggaat ttcattttta attgcatgcg
    23641 tactaaccac caatataagg aaatcctcaa aattgtttta aatagtgccc tcgacatgta
    23701 atttcaggcg tttagctgta actatttata tcggtaacta ttttctacgc gtcatctctg
    23761 tagaatggcc accgccaaat ggcgaattct tattttttct cgcgggtaat acccttccac
    23821 tacttaagtc tttttaacgt attcaggtgg agattcagtt atgcaaatag tcatgtttga
    23881 caggcagtca atatttattc atggaatgaa aatcagttta cagcagcgta ttccaggagt
    23941 gagtattcag ggggtcagtc aggcagacga gttatggcaa aagctggaaa gttaccctga
    24001 agccttagtt atgctcgatg gcgatcagga tggtgagttt tgctactggt tgctgcaaaa
    24061 aaccgtggtg cagtttcctg aggttaaggt gttaatcacg gcgacggatt gcaataaacg
    24121 gtggttacag gaagttatcc attttaatgt gctggccatt gtgcctcgcg attcaaccgt
    24181 cgagacattt gcgttggcgg taaatagtgc ggcgatgggg atgatgtttc tgcctggaga
    24241 ctggcggacg acgccggaaa aggacattaa agatctcaaa tcgttaagcg cccgccagcg
    24301 ggagatttta accatgttag cagcgggcga atcaaataag gagatcggca gagcgctgaa
    24361 tatcagtacc ggaacggtaa aagcgcatct ggaatccctg tatcgccgtc tggaagtgaa
    24421 aaatcgcacc caggcggcaa tgatgttaaa tatctcctcc
//
LOCUS       DAJTXY010000051        24170 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTXY010000051 DAJTXY010000000
VERSION     DAJTXY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.051"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(12..584)
                     /locus_tag="OO566_002154"
     CDS             complement(12..584)
                     /locus_tag="OO566_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6605436.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            802..1662
                     /gene="tesB"
                     /locus_tag="OO566_002155"
     CDS             802..1662
                     /gene="tesB"
                     /locus_tag="OO566_002155"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6605437.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1711..2997)
                     /gene="amtB"
                     /locus_tag="OO566_002156"
     CDS             complement(1711..2997)
                     /gene="amtB"
                     /locus_tag="OO566_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6605438.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3027..3365)
                     /gene="glnK"
                     /locus_tag="OO566_002157"
     CDS             complement(3027..3365)
                     /gene="glnK"
                     /locus_tag="OO566_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6605439.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3546..5327)
                     /locus_tag="OO566_002158"
     CDS             complement(3546..5327)
                     /locus_tag="OO566_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6605440.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5320..7092)
                     /locus_tag="OO566_002159"
     CDS             complement(5320..7092)
                     /locus_tag="OO566_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6605441.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7122..7580)
                     /gene="decR"
                     /locus_tag="OO566_002160"
                     /pseudo
     CDS             complement(7122..7580)
                     /gene="decR"
                     /locus_tag="OO566_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            complement(7734..8552)
                     /gene="cof"
                     /locus_tag="OO566_002161"
     CDS             complement(7734..8552)
                     /gene="cof"
                     /locus_tag="OO566_002161"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6605442.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8652..10351
                     /locus_tag="OO566_002162"
                     /pseudo
     CDS             8652..10351
                     /locus_tag="OO566_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10416..11111
                     /gene="queC"
                     /locus_tag="OO566_002163"
     CDS             10416..11111
                     /gene="queC"
                     /locus_tag="OO566_002163"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6605443.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11163..11561)
                     /gene="fadM"
                     /locus_tag="OO566_002164"
     CDS             complement(11163..11561)
                     /gene="fadM"
                     /locus_tag="OO566_002164"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6605444.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11655..12026)
                     /locus_tag="OO566_002165"
     CDS             complement(11655..12026)
                     /locus_tag="OO566_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6605445.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNLLVERNLAVLTL"
     gene            complement(12177..14048)
                     /gene="ppiD"
                     /locus_tag="OO566_002166"
     CDS             complement(12177..14048)
                     /gene="ppiD"
                     /locus_tag="OO566_002166"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6605446.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14240..14512)
                     /gene="hupB"
                     /locus_tag="OO566_002167"
     CDS             complement(14240..14512)
                     /gene="hupB"
                     /locus_tag="OO566_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6605447.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14721..17075)
                     /gene="lon"
                     /locus_tag="OO566_002168"
     CDS             complement(14721..17075)
                     /gene="lon"
                     /locus_tag="OO566_002168"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6605448.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17263..18537)
                     /gene="clpX"
                     /locus_tag="OO566_002169"
     CDS             complement(17263..18537)
                     /gene="clpX"
                     /locus_tag="OO566_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6605449.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18663..19286)
                     /gene="clpP"
                     /locus_tag="OO566_002170"
     CDS             complement(18663..19286)
                     /gene="clpP"
                     /locus_tag="OO566_002170"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6605450.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19533..20831)
                     /gene="tig"
                     /locus_tag="OO566_002171"
     CDS             complement(19533..20831)
                     /gene="tig"
                     /locus_tag="OO566_002171"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6605451.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21175..21492)
                     /gene="bolA"
                     /locus_tag="OO566_002172"
     CDS             complement(21175..21492)
                     /gene="bolA"
                     /locus_tag="OO566_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6605452.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21797..22375
                     /locus_tag="OO566_002173"
     CDS             21797..22375
                     /locus_tag="OO566_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6605453.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22419..23894
                     /gene="ampG"
                     /locus_tag="OO566_002174"
     CDS             22419..23894
                     /gene="ampG"
                     /locus_tag="OO566_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6605454.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5664 a         6660 c         5829 g         6017 t
ORIGIN      
        1 gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt cacctgagtt
       61 ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg cacggcggtt
      121 tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat caccggctga
      181 acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact caacagaata
      241 cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa tggtgactgt
      301 ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc ggctaacgac
      361 gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt ctgacggatc
      421 cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc tgaaatagac
      481 gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca agccgccaac
      541 gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt ctccttatta
      601 tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa tagtaacgtc
      661 aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt cagactattg
      721 cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct tgctgcggca
      781 gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac attgttaaat
      841 ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg tttacgccag
      901 gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac cgtcccggaa
      961 gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag taagaaaccg
     1021 attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg ccgggttgct
     1081 gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc accagaagcg
     1141 ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct gccttcggaa
     1201 acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga taaattcatc
     1261 tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa aggtcacgtc
     1321 gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccaga tgacctgcgc
     1381 gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt agctctacag
     1441 ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga ccattccatg
     1501 tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga gagcacctcg
     1561 gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg cgtgctggtt
     1621 gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag ccggaggtga
     1681 aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct cgccgtggct
     1741 gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa ccgtcagatc
     1801 cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa tggcgatgct
     1861 ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc ccacgccgcc
     1921 cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac aaacgccgtg
     1981 cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca tggtaacgcc
     2041 ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa ccccaatgta
     2101 gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg ctcccagcag
     2161 agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg ccgccgccgt
     2221 tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc ccgctgaccc
     2281 ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga ccatcggcag
     2341 gttgtgcggt ttaaacgcct ctttaccgaa gcccacgcgt ttgcctatca gatacgcgcc
     2401 caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga aatccagcgc
     2461 accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa tgtaagagag
     2521 cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt ccgccagcgc
     2581 cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta cgtggatata
     2641 ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca accagttaat
     2701 gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa ccacccagag
     2761 aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca cgtttttgcc
     2821 gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca gcaccagcgc
     2881 agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg cagccattac
     2941 cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta tcttcatttt
     3001 tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc gccggtacga
     3061 atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat ttttccggtg
     3121 taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc aatcgccaca
     3181 tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta cagctcggca
     3241 tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg aataccaatg
     3301 gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac ggtcaccagc
     3361 ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag aaggtattgc
     3421 aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa gattgaaaat
     3481 gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat gaaatattgc
     3541 gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc ttcgcccgca
     3601 agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg ctggtgagtg
     3661 ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc aataattgtt
     3721 gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc cagagcatgt
     3781 tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag gatcaggatt
     3841 tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc gactgagaga
     3901 ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg cgccagctca
     3961 gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc ccgccccagc
     4021 gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac cattgccacg
     4081 ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag gtgaatctca
     4141 ccttccgtta gcgggtaata gcccatcaat aaactggcaa gggtactttt accactgccg
     4201 gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat gttctttagc
     4261 accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt gccactctgt
     4321 aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc aaacacgcgc
     4381 tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc gattaagggt
     4441 tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc aatggtgccg
     4501 ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga aaacagactc
     4561 agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc catatagtgt
     4621 gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa ctgctggata
     4681 acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag ataggcgcgc
     4741 acacggcgga caatcggcgt gttgtaacgc tggtatatca ccattaccac cagcaccacc
     4801 gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat cgccaccagc
     4861 atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac gtagagatcg
     4921 cggatcactt cagtgtcatt agtgacgcgg gagatcacct gcccgacggg ttgggtatca
     4981 aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa ctgttgcact
     5041 acgcctactg ccgcccgatt aaacagcagc gactgagcgt aatgtagccc ggcggcaaac
     5101 agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg caggttattt
     5161 ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac ttctgccgcc
     5221 gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg cgaaccatac
     5281 gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga cggcctcctc
     5341 gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat acatatcgcg
     5401 ataccagccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga tatgtccgtg
     5461 ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg cactgatgat
     5521 taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct ctgtgcgtcc
     5581 gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta ataacgctcg
     5641 agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct cgcccacttc
     5701 tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta accgcgcgac
     5761 atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat tcgccacggt
     5821 gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc agctatcgag
     5881 ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga cgtcgaaatg
     5941 acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc aaatacccag
     6001 catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt cagtctgcgg
     6061 atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca cgagttcgct
     6121 accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt atgcagcact
     6181 accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa tcatcagacc
     6241 taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca ccaccatcca
     6301 actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga tggtcgggtc
     6361 gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt cggcatccgc
     6421 ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc ggatactggt
     6481 gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca gcttaaagcg
     6541 ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca tcggtaacag
     6601 ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca cagcgcagcc
     6661 cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca cgacgcgatc
     6721 gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt aaaactccgg
     6781 gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca gttggtaaga
     6841 cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa cggcaatcag
     6901 caccatggtg gcgatccaca tcaggatcag cccggtagta aagtgttgtt ctgtcacgcc
     6961 atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga taatgacaag
     7021 caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc ttaattgagc
     7081 aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat gggtaaagaa
     7141 gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag ccccggcacg
     7201 ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac ctagacgcgc
     7261 atcagataat cgtattcacc agccatgcgc cagaacccca gcacttctgg catttcggta
     7321 accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt tatcagcaca
     7381 aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt gccgataagg
     7441 ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt cagattaacg
     7501 gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc cagcagctta
     7561 cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt taattctatt
     7621 ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc acaaaatgac
     7681 aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc tcgttattcg
     7741 ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga gacagcctga
     7801 tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg cggcatcgca
     7861 ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt catcgcatca
     7921 ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagcac cgtcaatgca
     7981 gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga aaaacacaaa
     8041 tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag atcgtcgtga
     8101 tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac atcgattatc
     8161 tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc tttcccggta
     8221 aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg ctgatacagc
     8281 accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc ttccagagaa
     8341 tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc cccgagaata
     8401 tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc gcgttcacgc
     8461 agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat caataaagtg
     8521 ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg acacggttaa
     8581 tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa ataatattgt
     8641 tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg tggcaacctt
     8701 cagccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt ttttgcagcg
     8761 aacgccatgt tcgtacgctg ttgcgtcagg cacaggaggc gggatggctg gagtggcagg
     8821 cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgccg gaatcgctac
     8881 gcaatgcgat gatggaacag gcactggaaa ccggaaagca gcaagatgtg ctggagctgg
     8941 cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc ggacaatggc
     9001 aaacgataca cccacgttgc gtattcccta ctatcgcccg ctcgaaccgc tacaaccagg
     9061 ctttttgccc ggccgtgccg agcagcatct cgccgggcag atattttccg gcctgacccg
     9121 cttcgataat aatactcagc gcccgattgg cgatttagcg catcactggg aaacctctac
     9181 tgacgggtta cgctgggact tttatcttcg ttcaacccta cactggcata acggcgatgc
     9241 agtaaaagcc tcacacttac accagcgatt attgatgctg ttacaactgc cagcactgga
     9301 tcaattattt attagcgtga agcgtattga agtcacccat ccgcagtgtc tgaccttctt
     9361 tttacatcgc cccgattact ggcttgcgca ccggctggcg agctattgca gccatctggc
     9421 gcatccgcaa ttcccactga tcggcactgg tccttttcgc ttaacacaat tcacagcgga
     9481 gctggtgcgc ctggagagcc atgattatta ccatttgcgt catccgctgc ttaaagcggt
     9541 tgagtactgg ataactccgc cgcttttcga aaaagatttg ggaaccagtt gtcggcatcc
     9601 cgtgcaaatc accatcggca aaccggagga gctgcaacgg gtcagccagg tcagtagtgg
     9661 catcagttta ggtttttgct atttaacgtt gcgcaaaagt ccccgactct ccctctggca
     9721 ggcgcgaaaa gtgatctcca ttattcatca atccggttta ttacaaacgt tagaagtcgg
     9781 agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc cgcactggca
     9841 agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcacc taccgataga
     9901 acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct gtgaactcac
     9961 aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac acgcagacct
    10021 catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc aatggctacg
    10081 ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc tgcaatcgac
    10141 gctggacgcg gtgcaagtaa tgcctgatga ggaaaaccga tttaatgccc tgaaagcggt
    10201 ttttagccag ttaatggcag acgcgacgct gacgccgctg ttcaactatc actatcgcat
    10261 tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt ttgaatttac
    10321 cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat cgcaggcgtt
    10381 accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc gttgtgttca
    10441 gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat gatgaagtcc
    10501 attgcgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg gcacgcgaac
    10561 tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg ctcaacgagc
    10621 tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa cctgaagccg
    10681 atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg ctggcggcaa
    10741 tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa acggatttct
    10801 ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc gtcagtttgg
    10861 gcatggcgaa agatattcgt tttgaaacgc cgctgatgtg gattgataaa gcggaaacct
    10921 gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg ttgacctgct
    10981 ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta cgcgccaacg
    11041 gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag cagaaaaccg
    11101 ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc accacaaaac
    11161 gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc taatgctttc
    11221 tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac ctgcccttcc
    11281 ggctccagtg taatgacctg gcttaagatg ccgcttttac cgtttaattg ctgcaactga
    11341 ctggtaatag ttaacaggtc gcttaatacc gctgggcgac gatagttaat attgatattg
    11401 accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct attttccaac
    11461 ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac gtgctggtaa
    11521 acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata accttacgtt
    11581 gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca aaaaattgcc
    11641 actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag caaattgccc
    11701 atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc ttcgcgataa
    11761 ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc tagctcttcc
    11821 gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt cgccggtact
    11881 gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt cggtttcgcc
    11941 acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga cagggtaatg
    12001 agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac agtggaatca
    12061 ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa aaggccgcga
    12121 aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct tcaggattat
    12181 tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt actcatcaga
    12241 gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc ttttttctga
    12301 tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac attaccttgc
    12361 atatcagtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag tgcaaacgcc
    12421 gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc aaatttcaga
    12481 ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag cagtttctca
    12541 gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt aacttgttcc
    12601 tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgacgcg cagcacgaat
    12661 gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccgacgcgcc gttttcacct
    12721 accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc ttccggcagg
    12781 ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg ctctgcaccg
    12841 gccagagact cggtttcgtt gcttgccgca tcgctcactt tctgctgcag cgcatagtac
    12901 gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac ttcgtctaac
    12961 gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc gaccgaagat
    13021 ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag ttcgtcgggg
    13081 atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat aatatcggca
    13141 gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc aagtaccgct
    13201 ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg cggctgggtg
    13261 aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac cggttgctgc
    13321 atcgttgcgg caaccagctt gatgtaactc acgcggaatt gttccggcgt catgaaattg
    13381 tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg cttcgccgcc
    13441 agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac cagtgccgcc
    13501 agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt aatcagctgt
    13561 tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat ccccatctgg
    13621 ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa ggctggggtc
    13681 gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc acgagcgtac
    13741 tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag ggttttcata
    13801 tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg catgcgatta
    13861 cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc attcactttt
    13921 gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa tatgaacgac
    13981 acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt gcgtaagctg
    14041 tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc aacgtatcga
    14101 tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac aagacacaga
    14161 aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa cttcgtcaca
    14221 tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct gcacggaagc
    14281 tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg cggccagtac
    14341 gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca tcatcccctt
    14401 ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca gccgcagctt
    14461 tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc actcttctct
    14521 tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc agcagttata
    14581 tcaggccagc catcccctta caacacccct taataagggc aagcccgatc cgctatctaa
    14641 cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct gtttttatta
    14701 gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga cggttcattt
    14761 tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc cagatcggca
    14821 attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat tagcactgtt
    14881 ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat cggcagtacc
    14941 tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg gttaccggtc
    15001 aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc tttcggcgtc
    15061 gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg gttgatcccc
    15121 agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc ctgcatcact
    15181 tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc ggtttcaatg
    15241 gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctgacc gacacggttt
    15301 tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg caggttatcg
    15361 ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac cgctttgcgg
    15421 cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt gtagtaacga
    15481 ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag tgcattacgt
    15541 tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc ttcggtataa
    15601 ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt catggagttc
    15661 gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta gtggtcgctg
    15721 aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg atcgcctcgc
    15781 atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt cacgcccact
    15841 ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg gcggtgacca
    15901 cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt acgcccggtg
    15961 gctttggcaa tggactgacc aagagaggtt ttacctaccc ccggcggccc taccaggcag
    16021 aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc aaggatgcga
    16081 tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc ctgacgcagg
    16141 tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc gatataacca
    16201 cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg caactctgct
    16261 tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg cttcagggct
    16321 tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc tttcatttgc
    16381 tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg gttgcgaatg
    16441 cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag atattccaga
    16501 cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt cagcggcata
    16561 tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag cgacgtcagc
    16621 acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct gattgcagta
    16681 cgcaccagca cttcctgttc ccgctcatca atggtcggcg actccagata ctccgccttc
    16741 gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg taacccctcg
    16801 accagcactt tgacggtgcc gtcaggcagt ttcagcatct gcaatataga ggccacggtc
    16861 ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt ctgcgcgacc
    16921 agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga tttttcccgc
    16981 ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag cggcaatacg
    17041 gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag tttaatttcc
    17101 gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt acgtcagtat
    17161 atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa ggggagataa
    17221 aatcccccct ttttgtttaa ctaattgtat gggaatggtt aattattcac cagatgcctg
    17281 ttgcgcttcc ggcttaccat aaatcagcaa cggtttgctt tgaccatcaa ttaccgactc
    17341 gtcaataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg tatcgagcag
    17401 tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca tcgctttctt
    17461 agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca gattaaacag
    17521 cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa tcagagcttc
    17581 ttcgctcagt tcattcaacg ttgctacaac cggcagacga ccaatgaact cagggataag
    17641 accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt cgcttgcttt
    17701 gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt ctacacggtg
    17761 ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga tcttagaggt
    17821 atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg gaacagcagc
    17881 taccgtacct tcgatcagtt tcaacagtgc ctgctgtact ccttcaccgg aaacgtctcg
    17941 ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga tgtagacaat
    18001 cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct gaatgatgtt
    18061 ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg ccatggtgaa
    18121 cgggacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg aaccggtcgg
    18181 accgatcagc agaatgttac ttttgcccaa ctcgacacca ttgctggtat cgccgttgcg
    18241 cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct gttcctggcc
    18301 gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg cactgcgttc
    18361 acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata aatcaacaca
    18421 ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat gctggctttt
    18481 gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat ctgtcatggg
    18541 tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca ttctcaggcg
    18601 caagccgctt acagcgttgt gccgccctag ataagtatag cggcacagtt gcgcctctgg
    18661 catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc ttcaggggcg
    18721 gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg acccgtatga
    18781 agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgagc atgaatttcg
    18841 atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac gcgcgaattc
    18901 ggcaggcaaa aacgtttacc ttttgcccct gcggtcaaca agaaagcgcc catcgaggcc
    18961 gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt gtcatagata
    19021 gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat atctttttct
    19081 gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat gtggtcttca
    19141 acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat atcaaaagag
    19201 cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg gggtgcaaag
    19261 ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa aaaaacctgc
    19321 tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat gggtacggca
    19381 tcaccatatt tcaagcataa caatcttttg ttgttacgct aacaccgaaa gaggcttttc
    19441 gcacggctcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg tgacgggcct
    19501 ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat cagctcgttg
    19561 aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac agcctgttct
    19621 tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta gaactcgata
    19681 acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt cacgcgctct
    19741 tcgtcagctt tcagctcgtt ggtgcggata acttcgccca gcagcaggcc aacaactacg
    19801 cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg tttttcgttg
    19861 ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc gatcagcgca
    19921 gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga cttaacgcgg
    19981 ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc gcgcagacct
    20041 tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag ttccggcagt
    20101 tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc tttcaggttt
    20161 tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc agctttgtgg
    20221 cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc cagtacgaaa
    20281 tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa gtcgatggtt
    20341 acgcggtctt ctgcttcaac agcgccgtct ttttctttcc aggtcgcctg ctgtttacgc
    20401 agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg tttttcaact
    20461 tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc tacagagtaa
    20521 gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc agccggattg
    20581 attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc cagaacgtcc
    20641 tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt gcctttgcgg
    20701 aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac agcggtctcg
    20761 atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt ggtttcaact
    20821 gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt accccgcaag
    20881 cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat tccctgttac
    20941 cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc acttttatga
    21001 gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg caattttcgt
    21061 ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc aggccgtttt
    21121 tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc gtttttacgc
    21181 gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca acccttccca
    21241 ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga gttcctccgc
    21301 taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa aacgatcgct
    21361 gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac gatagctttc
    21421 atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt ctatccgctc
    21481 acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc cctttaaata
    21541 ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt tcgtcctggt
    21601 ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc ctcgcatcag
    21661 cgtttctttt accaggggtg attatttcgt caacatgatg aatttacatg ccttacccac
    21721 ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac gcttaagagt
    21781 ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc tgtttatgct
    21841 tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta cactgccaca
    21901 gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc agcgtacaga
    21961 tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg cctcccgcga
    22021 tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg gttacatggt
    22081 tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg ctgacgtgtc
    22141 ccagggcaac gtgcgctaca acatcgctac caaagcggat atcgccatca tcgctaccgc
    22201 gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg aaggtgcgtt
    22261 ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg ataccatcgc
    22321 tgatatgtct caggacacca gcattcacga attcatcaag cagaacgcgc gttaatttct
    22381 gccctctggc ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa tatttacgta
    22441 tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc gggctaccgc
    22501 tcgccctgac atccggcacc ttacaggcct ggatgacggt cgaaaatatc gatctcaaaa
    22561 ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc tggtcaccgc
    22621 tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg ctcgccacgc
    22681 aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaaccaggc acccaactcc
    22741 gctggatggc ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag gatattgtct
    22801 tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc gcggcaatca
    22861 gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cctagccctg tggctggcag
    22921 ataaatggtt gggctggcag ggcatgtact ggttgatggc ggcgctgttg atcccctgta
    22981 ttatcgcgac gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc aaaacgctgg
    23041 aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc tggcttattt
    23101 tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg acaaccacgt
    23161 ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac aaaacgcttg
    23221 gcttactggc gaccattgtt ggcgcattgt acggtgggat tttgatgcag cgcctgtcgc
    23281 tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct ggttactggc
    23341 tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt ttcgaaaacc
    23401 tctgtggcgg gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg tgtaataagt
    23461 cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtaggg cgagtttatg
    23521 tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc tatctattct
    23581 ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag acgcttgaat
    23641 atacacgagt aaatgacaac tttatctccc gtaccgaata tccggcaggt tatgcctttg
    23701 ccatgtggac actggcggcg ggcgtcagcc tgttggccgt gtggttactt ctgttgacga
    23761 tggacgcgct ggatttgacg cacttctctt tcctgcctgc tctgctggaa gtcggggttt
    23821 tagtcgccct ttctggcgtc gtgcttggtg gtttgctgga ttatctggcg ctacgaaaaa
    23881 cgcatctgac gtaatctgta aatattattt agctgtagta atcactcgcc gtaattatta
    23941 cggctaaata aacatcagca tcgcttatta atatttgttc ttttgtgcgg cttagcgttt
    24001 ggaaattatt ggcgccattt ataaattcta tttttcttac acgattcagc taatgagtct
    24061 ttattttctc accacccagt tgtcactcta atgataatta tttgttaaat aattgtttta
    24121 tttcgcattg gttataccaa ttgcccgcca agactccttc tgcactcccc
//
LOCUS       DAJTXY010000052        24105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTXY010000052 DAJTXY010000000
VERSION     DAJTXY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.052"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..1260
                     /gene="glpK"
                     /locus_tag="OO566_002175"
     CDS             <1..1260
                     /gene="glpK"
                     /locus_tag="OO566_002175"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6605455.1"
                     /translation="RETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLEDYISS
                     NTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVTDYT
                     NASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPISGI
                     AGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGEVNY
                     ALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLSAPYWDP
                     YARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAVANN
                     FLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFRPGI
                     ETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            1490..2500
                     /gene="glpX"
                     /locus_tag="OO566_002176"
     CDS             1490..2500
                     /gene="glpX"
                     /locus_tag="OO566_002176"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6605456.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLNRKDPEMQVH
                     IL"
     gene            2597..3343
                     /gene="fpr"
                     /locus_tag="OO566_002177"
     CDS             2597..3343
                     /gene="fpr"
                     /locus_tag="OO566_002177"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6605457.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(3348..3776)
                     /gene="uspD"
                     /locus_tag="OO566_002178"
     CDS             complement(3348..3776)
                     /gene="uspD"
                     /locus_tag="OO566_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6605458.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLENKSDNKLYKLTKNIQCPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(3803..4102)
                     /locus_tag="OO566_002179"
     CDS             complement(3803..4102)
                     /locus_tag="OO566_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6605459.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(4314..4754)
                     /locus_tag="OO566_002180"
                     /pseudo
     CDS             complement(4314..4754)
                     /locus_tag="OO566_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            4855..5454
                     /locus_tag="OO566_002181"
     CDS             4855..5454
                     /locus_tag="OO566_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6605460.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            5562..6329
                     /gene="tpiA"
                     /locus_tag="OO566_002182"
     CDS             5562..6329
                     /gene="tpiA"
                     /locus_tag="OO566_002182"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6605461.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(6384..7139)
                     /gene="cdh"
                     /locus_tag="OO566_002183"
     CDS             complement(6384..7139)
                     /gene="cdh"
                     /locus_tag="OO566_002183"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6605462.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKSVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(7246..8235)
                     /gene="sbp"
                     /locus_tag="OO566_002184"
     CDS             complement(7246..8235)
                     /gene="sbp"
                     /locus_tag="OO566_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6605463.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(8555..9517)
                     /gene="pfkA"
                     /locus_tag="OO566_002185"
     CDS             complement(8555..9517)
                     /gene="pfkA"
                     /locus_tag="OO566_002185"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6605464.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(9698..10600)
                     /gene="fieF"
                     /locus_tag="OO566_002186"
     CDS             complement(9698..10600)
                     /gene="fieF"
                     /locus_tag="OO566_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6605465.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(10748..11248)
                     /gene="cpxP"
                     /locus_tag="OO566_002187"
     CDS             complement(10748..11248)
                     /gene="cpxP"
                     /locus_tag="OO566_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6605466.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            11398..12096
                     /gene="cpxR"
                     /locus_tag="OO566_002188"
     CDS             11398..12096
                     /gene="cpxR"
                     /locus_tag="OO566_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6605467.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            12093..13466
                     /gene="cpxA"
                     /locus_tag="OO566_002189"
     CDS             12093..13466
                     /gene="cpxA"
                     /locus_tag="OO566_002189"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6605468.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(13572..14246)
                     /gene="yiiM"
                     /locus_tag="OO566_002190"
     CDS             complement(13572..14246)
                     /gene="yiiM"
                     /locus_tag="OO566_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6605469.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(14395..15378)
                     /gene="kdgT"
                     /locus_tag="OO566_002191"
     CDS             complement(14395..15378)
                     /gene="kdgT"
                     /locus_tag="OO566_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6605470.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(15638..16258)
                     /gene="sodA"
                     /locus_tag="OO566_002192"
     CDS             complement(15638..16258)
                     /gene="sodA"
                     /locus_tag="OO566_002192"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6605471.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            16543..17577
                     /gene="rhaT"
                     /locus_tag="OO566_002193"
                     /pseudo
     CDS             16543..17577
                     /gene="rhaT"
                     /locus_tag="OO566_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(17574..18440)
                     /gene="rhaR"
                     /locus_tag="OO566_002194"
     CDS             complement(17574..18440)
                     /gene="rhaR"
                     /locus_tag="OO566_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6605472.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(18496..19332)
                     /gene="rhaS"
                     /locus_tag="OO566_002195"
     CDS             complement(18496..19332)
                     /gene="rhaS"
                     /locus_tag="OO566_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6605473.1"
                     /translation="MTVLRSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            19620..21089
                     /gene="rhaB"
                     /locus_tag="OO566_002196"
     CDS             19620..21089
                     /gene="rhaB"
                     /locus_tag="OO566_002196"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6605474.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            21086..22345
                     /gene="rhaA"
                     /locus_tag="OO566_002197"
     CDS             21086..22345
                     /gene="rhaA"
                     /locus_tag="OO566_002197"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6605475.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            22587..23411
                     /gene="rhaD"
                     /locus_tag="OO566_002198"
     CDS             22587..23411
                     /gene="rhaD"
                     /locus_tag="OO566_002198"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6605476.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            23421..23735
                     /gene="rhaM"
                     /locus_tag="OO566_002199"
     CDS             23421..23735
                     /gene="rhaM"
                     /locus_tag="OO566_002199"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6605477.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            24036..>24105
                     /locus_tag="OO566_002200"
     CDS             24036..>24105
                     /locus_tag="OO566_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6605478.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLK"
BASE COUNT         5981 a         6294 c         6131 g         5699 t
ORIGIN      
        1 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
       61 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
      121 tatatcagca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
      181 tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga attgctgttt
      241 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
      301 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
      361 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
      421 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
      481 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
      541 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
      601 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
      661 gaaggtgcgg tgtttatggc aggcacatcc attcagtggc tgcgcgatga gatgaagttg
      721 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
      781 tatgtggttc cggcatttac cgggctgagt gcgccgtact gggacccgta tgcgcgcggg
      841 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
      901 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
      961 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
     1021 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
     1081 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
     1141 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
     1201 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
     1261 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
     1321 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
     1381 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
     1441 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
     1501 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
     1561 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
     1621 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
     1681 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
     1741 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
     1801 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
     1861 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
     1921 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
     1981 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
     2041 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
     2101 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
     2161 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
     2221 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
     2281 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
     2341 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
     2401 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
     2461 gaatcgcaaa gacccggaaa tgcaagtgca cattctctga gtgatttgat cgattgagcc
     2521 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
     2581 aaaacaggag aaaaaaatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
     2641 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
     2701 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
     2761 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
     2821 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
     2881 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
     2941 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
     3001 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
     3061 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
     3121 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
     3181 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
     3241 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
     3301 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
     3361 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
     3421 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
     3481 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcgga
     3541 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctc caacaattga
     3601 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
     3661 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
     3721 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
     3781 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
     3841 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
     3901 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
     3961 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
     4021 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
     4081 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
     4141 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
     4201 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
     4261 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
     4321 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
     4381 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
     4441 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
     4501 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
     4561 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
     4621 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
     4681 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
     4741 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
     4801 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
     4861 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
     4921 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
     4981 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
     5041 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
     5101 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
     5161 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
     5221 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
     5281 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
     5341 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
     5401 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
     5461 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
     5521 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
     5581 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
     5641 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
     5701 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
     5761 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
     5821 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
     5881 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
     5941 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
     6001 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttatg
     6061 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
     6121 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
     6181 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
     6241 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
     6301 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
     6361 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
     6421 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
     6481 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
     6541 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
     6601 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
     6661 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
     6721 tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
     6781 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
     6841 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
     6901 gacatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
     6961 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
     7021 tcaaggacac tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
     7081 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
     7141 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
     7201 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
     7261 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
     7321 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
     7381 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
     7441 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
     7501 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
     7561 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
     7621 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
     7681 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
     7741 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
     7801 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
     7861 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
     7921 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
     7981 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
     8041 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
     8101 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
     8161 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
     8221 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
     8281 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
     8341 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
     8401 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
     8461 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
     8521 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
     8581 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
     8641 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
     8701 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
     8761 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
     8821 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
     8881 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
     8941 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
     9001 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
     9061 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
     9121 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
     9181 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
     9241 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
     9301 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
     9361 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
     9421 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
     9481 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
     9541 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
     9601 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
     9661 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
     9721 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
     9781 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
     9841 agtcttccat ttccaaatgg atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
     9901 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
     9961 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    10021 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    10081 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    10141 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    10201 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    10261 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    10321 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    10381 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    10441 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    10501 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    10561 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    10621 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    10681 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    10741 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    10801 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    10861 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    10921 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    10981 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    11041 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    11101 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    11161 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    11221 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    11281 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    11341 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    11401 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    11461 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    11521 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    11581 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    11641 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    11701 tttaatgatc gtgagctggt ggcacgtatt cgcgcgatcc tgcgccgttc gcactggagc
    11761 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    11821 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    11881 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    11941 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    12001 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    12061 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    12121 tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg ttacccaagc
    12181 tcgattcacg ccagatgacc aagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    12241 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    12301 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    12361 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    12421 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    12481 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    12541 gcagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    12601 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    12661 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    12721 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    12781 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    12841 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    12901 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    12961 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    13021 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    13081 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    13141 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    13201 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    13261 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    13321 gcgatcgtga atctggcggt acaggattgg ggctggcaat tgttgaaacc gccattcagc
    13381 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    13441 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    13501 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    13561 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    13621 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    13681 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    13741 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    13801 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    13861 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    13921 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    13981 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    14041 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    14101 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    14161 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    14221 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    14281 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    14341 tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttaaggaagc aggtttattt
    14401 caccgtacct aaaatttcga ttttcgctgc tctggctttg actttacgtg accagataga
    14461 ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc gtcgctacca gcgaagttgc
    14521 tgccggagcc atcggtttaa acgcaggcac catttctgca atcagcacag gtgtcgctac
    14581 cgcggcccct gcggaactgg aagcggcaat tccggcagtg ccatcgccac cgccaatcaa
    14641 tttatcggcg ataatcaacg gaataccggt cacgataatt attgccacac ccaacaggat
    14701 ccccagcaaa ccagtctggg caatcacagt caaatcaatg gtattgccca gcgcgaaggc
    14761 aaagaatgga atcagcgttt gcaccgcttt gctgaaaaat tctcgtaatt cagggtcaag
    14821 attccccagg gcaaagccca ccaggaacgg taatactgcg ccgacgaaaa catgcggttc
    14881 aaacgaggca atcccggcag tgcccagaat aatcatcgtc atgagcggcc cggactccaa
    14941 cgacatcaac acaaatgccc cagcttcttc tttcgtaccg tactgctgca tgatggaagc
    15001 gtaaagtccg ccgttggtca tatccatcgc cgccaccagc gccagcgttg aaagtccggc
    15061 aaagaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg ccgcaaccac
    15121 ccacgcgacg gcaattttag ttaccaccag cgtaccggat ttacgcagta ccgttcccgt
    15181 cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa tgggcaccgt
    15241 accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg gcgagaaggt
    15301 gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga ttttctcaat
    15361 cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct gatgcgtaat
    15421 tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta atcaataaaa
    15481 gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt ataggatcat tgtttttatt
    15541 ttggaattgt tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt aagctgatat
    15601 gcggcctata cgcctcattg cagcaggcgg caaatgatta ttttttcgcc gcaaaacgtg
    15661 ccgctgcttc gtcccagttc accacgttcc agaactcttt gatgtagtcc ggacggcggt
    15721 tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc gggaagccgg
    15781 aagcgccaga aatagcttca cccatcagcg gagaatcctg gttagcagta gaaaccaccg
    15841 ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg gaagctgccg
    15901 ctttttcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg atagccgctt
    15961 tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg ctgtggttag
    16021 cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc tggtccagtt
    16081 tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc agcgccgcgt
    16141 tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg gtctgcttat
    16201 cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta tagctcatat
    16261 tcatctccag tattgtcggg cggccgattg ttaatgccgc gtaagcagtt ggtttattat
    16321 agttaattaa atgatattga aaatgattat caatgccgca cttttcgtaa gggtatggtt
    16381 ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact taatgccgtg attgccagta
    16441 aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag cacgattttt acactcatct
    16501 cgtcggagat gtgaagcgac gaaaaatgat gaggataaga agatgagtaa cgcgattacg
    16561 atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta cgctccgttc
    16621 aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag tcggtgggat tgtttcgtgg
    16681 attattctgc cgtgggccat cagcgccctg ttactaccgg atttctgggc gtattacagc
    16741 tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg ggggatcggt
    16801 aatatcaact atggcctgac catgcgttat ctcggcatgt cgatgggaat tggcatcgcc
    16861 attggcatta cgttgattgt cggtacgctg atgacgccaa ttatcaacgg caatttcgat
    16921 gtgctgatta acaccgaagg cggacgcatg acgttactcg gcgttctggt ggcgctgatt
    16981 ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc gcaagatggg cattaaagcc
    17041 gaagagttca atctgaaaaa agggctggtg ctggcggtga tgtgcggcat tttctctgcc
    17101 gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc cgcacttggc
    17161 gtcgatccac tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg cggcgcgatc
    17221 attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc gctaaaagcc
    17281 gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt tactctcggc actgggcggg
    17341 ttgatgtggt atctgcaatt ctttttctat gcctagggcc acgcccgcat tccggcgcag
    17401 tatgactaca tcagttggat gctgcatatg agtttctatg tattgtgcgg cggtatcgtc
    17461 gggctggtgc tgaaagagtg gaacaataca ggacgccgtc cggtaacggt gttgagcctc
    17521 ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc gaattaatct
    17581 ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc
    17641 accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg
    17701 cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag atattgattg
    17761 atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc
    17821 tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag ccgggtaatc
    17881 agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt gtaacgatgg
    17941 cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacggc
    18001 acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc
    18061 acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg aatcgctccc
    18121 tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg caaaaccaga
    18181 tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag atcgccacgg
    18241 gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca gacaatcacc
    18301 agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg gtcagccaca
    18361 gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc
    18421 accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg
    18481 ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga
    18541 ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg
    18601 ataggcgatg tcagtgacgc tggcctcgct gtggcgtagc agatgtcggg ctttcatcag
    18661 tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg
    18721 tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc
    18781 aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt
    18841 ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact
    18901 ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg
    18961 cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc
    19021 ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag
    19081 cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat gatcgcgtac
    19141 gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt
    19201 gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg
    19261 cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat ccacactacg
    19321 taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc
    19381 aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata
    19441 cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag caaattgtga
    19501 gcatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga
    19561 aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag gatcacatta
    19621 tgacctttcg caattgtgtc gccgtagatc tcggcgcatc cagtgggcgc gtgatgctgg
    19681 cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt tttaacaatg
    19741 ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa agtgccattc
    19801 gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt gggattgata
    19861 cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc ctgcccgttg
    19921 cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa ctcggcaaac
    19981 gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt tatcagttgc
    20041 gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct ctgctgatgc
    20101 cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc aatgccacca
    20161 ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg gcgtggagcg
    20221 gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt gggcactgga
    20281 tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat accgccagcg
    20341 cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct ggcacctggt
    20401 cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg gcagccaaca
    20461 tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt atgggcttat
    20521 ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg cttatcgccg
    20581 cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat cgctttatca
    20641 atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg caaccgatcc
    20701 cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg ctgtatgccg
    20761 atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg catattgtcg
    20821 gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc ggtattcggg
    20881 tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag ttaattacgc
    20941 tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc gcgaatctga
    21001 ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag attcactcta
    21061 cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct gggagctggc
    21121 gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc aacttgatcg
    21181 tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg aaaacccgga
    21241 aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag cgcgtaatgc
    21301 cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc cgaaacggct
    21361 taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc agatcaaacc
    21421 agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc tggattttaa
    21481 cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc atcccgacga
    21541 cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt cggcctattt
    21601 tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg gtatgaaaga
    21661 tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg atgaggtgat
    21721 cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat tgtttggcat
    21781 tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg ccaccagccg
    21841 ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga tttccgacaa
    21901 gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc gtccggttcg
    21961 ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg ccagtgagat
    22021 tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg atgcctctat
    22081 caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc tgctgcgtgc
    22141 gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg
    22201 tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg aaatgtattg
    22261 ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg cttatgagaa
    22321 agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacaagcgc cttatccggc
    22381 ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc ctgaaagggc
    22441 gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg gacagtcccc
    22501 tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg
    22561 caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg tttgtccagg
    22621 gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc aacggcggca
    22681 acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac ttccacccac
    22741 aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg ccgtttattg
    22801 tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct aacttaggcg
    22861 tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta accaacgaag
    22921 ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc attaaagcca
    22981 ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc gccctcacct
    23041 atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc agcaccgaat
    23101 gtctggcagt attcccggat ggcgttggca ttctgccgtg gatggtgccc ggcacggacg
    23161 aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg tggcccttcc
    23221 acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc gacaccgcag
    23281 aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa cagaccatca
    23341 gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc
    23401 tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta aaccccgacg
    23461 cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa gcagtgttga
    23521 aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat ctgctgtttg
    23581 ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact gatgtttgcc
    23641 aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac agcccggtga
    23701 gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg gagatgcctc
    23761 cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc ccggtaagtt
    23821 atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca ttaatgattc
    23881 atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc tttcactctg
    23941 tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc tccaccatta
    24001 caattgatta aaatatattt tatggagtcc gattaatggc agctcttact gcaagctgta
    24061 ttgacctgaa tattcagggc aatggcgctt attccgttct gaagc
//
LOCUS       DAJTXY010000053        23926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTXY010000053 DAJTXY010000000
VERSION     DAJTXY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.053"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..336
                     /locus_tag="OO566_002201"
     CDS             <1..336
                     /locus_tag="OO566_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6605479.1"
                     /translation="KGNGIPANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGKELPQN
                     LPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKA
                     LNELEHQTK"
     gene            389..2119
                     /locus_tag="OO566_002202"
     CDS             389..2119
                     /locus_tag="OO566_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605480.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWMKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(2179..2670)
                     /gene="yajQ"
                     /locus_tag="OO566_002203"
     CDS             complement(2179..2670)
                     /gene="yajQ"
                     /locus_tag="OO566_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6605481.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2838..3749
                     /gene="panE"
                     /locus_tag="OO566_002204"
     CDS             2838..3749
                     /gene="panE"
                     /locus_tag="OO566_002204"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6605482.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3712..4302
                     /gene="yajL"
                     /locus_tag="OO566_002205"
     CDS             3712..4302
                     /gene="yajL"
                     /locus_tag="OO566_002205"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6605483.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4356..5804)
                     /gene="thiI"
                     /locus_tag="OO566_002206"
     CDS             complement(4356..5804)
                     /gene="thiI"
                     /locus_tag="OO566_002206"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6605484.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            6010..6252
                     /gene="xseB"
                     /locus_tag="OO566_002207"
     CDS             6010..6252
                     /gene="xseB"
                     /locus_tag="OO566_002207"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6605485.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            6252..7151
                     /gene="ispA"
                     /locus_tag="OO566_002208"
     CDS             6252..7151
                     /gene="ispA"
                     /locus_tag="OO566_002208"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6605486.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            7176..9038
                     /gene="dxs"
                     /locus_tag="OO566_002209"
     CDS             7176..9038
                     /gene="dxs"
                     /locus_tag="OO566_002209"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6605487.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            9093..10067
                     /gene="yajO"
                     /locus_tag="OO566_002210"
     CDS             9093..10067
                     /gene="yajO"
                     /locus_tag="OO566_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6605488.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEENSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(10121..10639)
                     /gene="pgpA"
                     /locus_tag="OO566_002211"
     CDS             complement(10121..10639)
                     /gene="pgpA"
                     /locus_tag="OO566_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6605489.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10617..11594)
                     /gene="thiL"
                     /locus_tag="OO566_002212"
     CDS             complement(10617..11594)
                     /gene="thiL"
                     /locus_tag="OO566_002212"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6605490.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDVLSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11672..12091)
                     /gene="nusB"
                     /locus_tag="OO566_002213"
     CDS             complement(11672..12091)
                     /gene="nusB"
                     /locus_tag="OO566_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6605491.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(12111..12581)
                     /gene="ribE"
                     /locus_tag="OO566_002214"
     CDS             complement(12111..12581)
                     /gene="ribE"
                     /locus_tag="OO566_002214"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6605492.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGSAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12670..13773)
                     /gene="ribD"
                     /locus_tag="OO566_002215"
     CDS             complement(12670..13773)
                     /gene="ribD"
                     /locus_tag="OO566_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6605493.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13777..14226)
                     /gene="nrdR"
                     /locus_tag="OO566_002216"
     CDS             complement(13777..14226)
                     /gene="nrdR"
                     /locus_tag="OO566_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6605494.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14377..14916
                     /locus_tag="OO566_002217"
     CDS             14377..14916
                     /locus_tag="OO566_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6605495.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15215..16099
                     /gene="tsx"
                     /locus_tag="OO566_002218"
     CDS             15215..16099
                     /gene="tsx"
                     /locus_tag="OO566_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6605496.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16276..16623)
                     /locus_tag="OO566_002219"
     CDS             complement(16276..16623)
                     /locus_tag="OO566_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6605497.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16752..17723)
                     /gene="secF"
                     /locus_tag="OO566_002220"
     CDS             complement(16752..17723)
                     /gene="secF"
                     /locus_tag="OO566_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6605498.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17734..19581)
                     /gene="secD"
                     /locus_tag="OO566_002221"
     CDS             complement(17734..19581)
                     /gene="secD"
                     /locus_tag="OO566_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6605499.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19609..19941)
                     /gene="yajC"
                     /locus_tag="OO566_002222"
     CDS             complement(19609..19941)
                     /gene="yajC"
                     /locus_tag="OO566_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6605500.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19964..21091)
                     /gene="tgt"
                     /locus_tag="OO566_002223"
     CDS             complement(19964..21091)
                     /gene="tgt"
                     /locus_tag="OO566_002223"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6605501.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDMHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(21146..22216)
                     /gene="queA"
                     /locus_tag="OO566_002224"
     CDS             complement(21146..22216)
                     /gene="queA"
                     /locus_tag="OO566_002224"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6605502.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22309..22882
                     /gene="acpH"
                     /locus_tag="OO566_002225"
                     /pseudo
     CDS             22309..22882
                     /gene="acpH"
                     /locus_tag="OO566_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(22887..>23926)
                     /gene="malZ"
                     /locus_tag="OO566_002226"
     CDS             complement(22887..>23926)
                     /gene="malZ"
                     /locus_tag="OO566_002226"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6605503.1"
                     /translation="DRHNLGTGGACHNSESPWRDWYLFSDDGTALDWLGYASLPKLDY
                     QSESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARNNMQHVAGITEAA
                     KETQSEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGFLANTDISYDPQQIDAQ
                     TCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIARLPLAVVWLFTWPGVP
                     CIYYGDEVGLDGKNDPSCRKPFPWQVEKQDTALFALYRRMIVLRKKSQALRRGGCQVL
                     YAEDNVVVFVRVLNQQRVLVAINRGEASEVVLPASPFLNAVQWQCKEGHGQLTDGILA
                     LPAISATVWMN"
BASE COUNT         6083 a         6270 c         5879 g         5694 t
ORIGIN      
        1 aaaggcaatg gtatacctgc aaacaatgaa aaatacctag catatctgca acaagctgca
       61 acactgggca gtagtaatgc ccaggcggaa attggctatt tatatctcat cggaaaagaa
      121 ctaccacaga atcttcctga tgctggtgtc tggttcaaaa aagcagctgc acaaggtaat
      181 tttgccgcac atttctatct gggaaggatg tatcagaatg gtgacggcgt tgagagaaat
      241 atggaaaaag ccaggtttca tctatctaat gcggcagaag gtggaataga acctgcgctt
      301 aaagcattaa atgaacttga acatcaaaca aaataaaacg ctcagggacc acgtaaggtt
      361 catcaaacag aaaaggatat agtttgcgat gaaagcgaaa tctactttgt tattaatgat
      421 tttatctgtt ttttattctc atcaggtttt atccagcgac cccactcagc cggatgttta
      481 ttctgtcgtc gagaaaaaac taaactctgc tttacctctg gaagaaaata ccaccttcag
      541 atcgcaggct gaatggttta atttgcaata tgaattatta gtatctggct accctgaacg
      601 tgcttacaat ttactgagca aacttgagac agaatcgaaa aacgctaaat attatctgga
      661 tttaactgcc tccagagtcg ctgaagaaga agcaccagag acagccttaa cttttttatc
      721 aaaaattggg ttaaataaac cggctactct tacatccttt gagagctata tcaatagctg
      781 gatgaaaagc aaacaacccg aaaaggctat gcagcttctc agcctcgatg agaatgccct
      841 gaattattat ttaccgactg tgttagtcgc atatcagatc gttcctgata aggcaataag
      901 tatttataat tctgtatatg gagataatgt tgttataccc tatcagcaag tcaccatgtt
      961 acttacagtt gcggagggtt atcaaacaaa aggcgatata aaaaacgcca ttcattatgc
     1021 cgataaggcg ctgaatatgt ttgataaagc tattgctgta gatagcagtc gtgaatccca
     1081 ttatagtgat gagtatttga aactcatgaa tatctacgcc gccaatggca acaaagaaaa
     1141 agcggtggtt ttatctcaac gtctacacaa agccattacc gaatctcaat cgaattactt
     1201 aagtactctg gaaggaattt tattgtttta tagaaataat gatatgcagc aagactatca
     1261 aaacagcctt tcaaattaca tagtatttat cgatgaaatt tttgcctttt ccccatcaac
     1321 aagaagtgaa ttaagcctca ttaatttatt aaattcatta aatgaggtgg aattgatgag
     1381 aaaacgtctg gcgtttctga tgacagcacc agaatacgct tgttatgata gtcagtattg
     1441 ttatgaagac aaagtcagcg cattaaaaat cctgtatcgt catcatgaca atgagctaat
     1501 ggacaaatat ttcacgactc ttcttaaaga gatcagtaat caaccattag aagactggga
     1561 tataataata agtaatactg gtgaaaaatt tagtgaagtg ggtctaacca aatatgctca
     1621 acatctcgca tctttcgctg aagaatttta tataaaagag caaaaatcac attctgaaag
     1681 tgaaatgaga ggactatttt catcacttgc cgaattatat agttttggta atgatactgc
     1741 cagcgcaaac cgagtgttcc atcagcatgt ccctgccttc aatacagatc atatgattca
     1801 atattttata aatgccaaag aatggaacaa tgccagggaa ttattgataa aagaggaaat
     1861 gttgggtatg cataatctga tattgttgga gaatatttgt atgcaaaaaa atgcagaatg
     1921 catggcacac attaccttta cgcttaataa attaacaaca caaccagcaa taacaaaaat
     1981 agatagcgtg ggtaatgagc aactttatca gattggcaaa atctaccata agttggaaat
     2041 taaacctgag ccagagcaac aggttttaat tcagtcgctt tatgaccggg catcaggaag
     2101 cgcctctgca actcaataaa tgcatggtca aaagggccac cacggtggcc ctgacaatca
     2161 gtagctatat ccagaaagtt aatcgcggaa gtttttgaac tggaacggct gaccgagatc
     2221 gccaccgcgc accatcgcca tgacagcctg caaatcatca cgagatttgc cagtcacgcg
     2281 gatctcatcg ccctgaattt gcgcctgcac tttcagtttg ctgtctttga tcatcttgac
     2341 gatttttttc tgagtggcgc tctcaatgcc ctgtttcagt ttcgcttcca caaaccaggt
     2401 tttaccgcta tgaacgatat tttccggtac atccagtgaa ctgccttcaa tgccgcgctt
     2461 cagcagcttg gcacgcagaa tatccagcaa ctgattgacc tggaagtcgg actcgctcaa
     2521 cactttgatg gttttgctgg cgtcgttcag ctcaaatgag gcttcaacgt tacggaagtc
     2581 aaaacgggac tccacttcgc gactcgcgtt atcgaccgcg ttacgtgctt cctgaagatc
     2641 aacttcagag acaatatcga aagatggcat cttttcctct cccttcattt ttgatgcgaa
     2701 gcataatacc tgcaaagtta aataaccaac agtcctgcac caggacgtat cccttaattg
     2761 tgcatagtcg caacagcatg cacaattaag ggatagcctt attgaacacc cggagtggtt
     2821 gtgggtgagg aggaacaatg aaaattaccg tattgggatg cggtgcctta gggcaattat
     2881 ggcttacagc actttgtaaa cagggtcatg aagttcaggg ctggctgcgc gtaccgcaac
     2941 cttattgtag tgtgaatctg gttgagacag atggttcgat atttaacgaa tcgctgaccg
     3001 ccaacgatcc cgattttctc gccaccagcg atctgctcct ggtgacgctg aaagcatggc
     3061 aggtttccga tgccgtcaaa agcctcgcat ccacactgcc tgtaactacg ccaatactgt
     3121 taattcacaa cggcatgggc accatcgaag aattgcaaaa cattcagcag ccattactga
     3181 tgggcaccac cacccatgcc gcccgccgcg acggcaatgt cattattcat gtggcaaacg
     3241 gaatcacgca tatcggtccg gcacggcaac aggatggcga ttacagttat ctggcggata
     3301 ttttgcaaac cgtgttgcct gacgttgcct ggcataacaa tattcgcgcc gagctgtggc
     3361 gcaagctggc cgtcaactgc gtgattaatc cactgactgc catctggaat tgcccgaacg
     3421 gtgaattacg tcatcatccg caagaaatta tgcaaatatg cgaagaagtc gcggcagtga
     3481 tcgaacgcga agggcatcat acttcagcgg aagatttgcg tgattacgtg atgcaggtga
     3541 ttgatgccac agcggaaaat atctcgtcga tgttgcagga tatccgcgcg ctgcgccaca
     3601 ccgaaatcga ctatatcaat ggttttctct tacgccgcgc ccgcgcgcat gggattgccg
     3661 taccggaaaa cacccgcctg tttgaaatgg taaaaagaaa ggagagtgaa tatgagcgca
     3721 tcggcactgg tttgcctcgc ccctggtagt gaagagactg aagccgtcac cacgatcgac
     3781 ctcttggttc gcggcggtat caaagtcacc actgccagcg tcgacagcga tggcaacctg
     3841 gcaattacct gctcgcgcgg cgtgaagctg ctggcggatg cgccgctggt cgaagtggct
     3901 gatggcgaat atgacgtgat cgtgctgcct ggtggcatta aaggcacgga gtgttttcgc
     3961 gatagcactc tgctggttga aaccgttaaa cagttccacc gttccgggcg tatcgtcgcg
     4021 gctatttgcg ccgcgccagc caccgtgctg gtgccgcacg atatctttcc gattggcaat
     4081 atgaccggct tcccgacgct gaaagacaaa attcccgctg aacaatggca ggacaagcgc
     4141 gtcgtctggg atgcacgggt aaaattgctg accagccagg ggccgggtac agctatcgac
     4201 tttggtctga aaattatcga cctgctggtt gggcgcgaga aagcccatga agtggcatca
     4261 caactggtga tggcggcagg gatttataat tattacgagt agtgtcggat gcggcaaacg
     4321 tcgcatctga ccagatgcga cgtaaaaact acaaattacg ggcgatatac cttcacattg
     4381 ttaaagcctt gctcgcgcag atagagcgcc tgcagacggc tcatcacccc gcgctcacac
     4441 cacagcagcc aggttttgtt ctggtcgaga tcgccaaatt tggtgctcag tttatagaac
     4501 ggcagagaaa ccacatcaat cccttcgact ttcagtggct tatcttcctg ttcatcgaca
     4561 gaacggatat cgagaatcac gtcgttcggg ccgaagccgt tgacggtttc cacttccacc
     4621 acttcctgct cggtctgctg ggcgatttcg cggatatcaa cgttattcgc ttcctcaacc
     4681 actttatcga gaatactgaa gtcgaacttc tcttcttccg cttcaatctt cgatttaacc
     4741 gctttcaccg tcgggctttt ggagatcacg ccacaatatt ccggcatcgt gcgagcaaag
     4801 tcttcggtgc caatctggcg agccaggttg atgatgtgct ctttgtcgta agaaatcagc
     4861 ggacgcagga tcagcgtgtc agagacgtta tcaatcaggc gcaggttggt cagcgtctga
     4921 ctggacacct ggccgagcgc ttcgccggtg accagcgcct gtacgccgta acgttcagcc
     4981 actttagatg cggcacgcac catcatacgt ttgaggataa cgcccatctg accgtcgtcg
     5041 attttctcga gaatttcccc gacaaccggt tcgaaattaa tggcgacaaa acgcacgcgg
     5101 tgggagctgc caaagcggtt ccacagataa tgcgccacct gacgcacgcc aatttcatgc
     5161 gctgcgccgc cgaggttaaa gaagcagtaa tgcacgcggc agccgcgacg catcaacata
     5221 taactggaaa caccggagtc gaaaccaccg gaaatgagcg acagcacatc ttcctgggtg
     5281 ccaatcggga aaccaccaat accttcgtag cgacctttaa tcagcaggag gcgatcgtct
     5341 tccacttcca gatgaaccgt tacatccggg ttagtcagct tcacgcgcgc ggattcaata
     5401 tgctgattta aaccgccgcc gacgtaacgt tccacatcaa tcgagctaaa atcatgtttg
     5461 ccacggcgct tcacgcgtac gcagaaggtt ttgccttcca gctgatcgcg atactgaacc
     5521 aacgctttct caaaaatatc gtgcatgtcg gtaaacggca cgtcttcgac ttcgagaata
     5581 tggtggatac ccggaatacg ggtcagggcg tcgcgaatag tcagacgctg gttttcatct
     5641 ttcgcgcgaa cttcgatgtt atcccagtgg cggacgacag cgagcgtctc atcatagtgc
     5701 tttaaaacgt tacgaatgtt cccggtaagg atttttataa agcgcaagcg cacagattgg
     5761 cttttgatgg tgatttccgg gaacaattta atgataaact tcatggcggc aatggttcgt
     5821 tggcaagcct taagcgactt gtatagggaa aaatacagca gcccacacct gcggctgcat
     5881 ccaggcgcgg aagtatacca ctaacatcgc tttgctgcgc acatcacctt accattgcgc
     5941 gttatttgct atttgccctg agtccgttac catgacgggg cgaaaaatat tgagagtcag
     6001 acattcatta tgccgaagaa aaatgaggcg cccgccagct ttgaaaaggc gctgagcgag
     6061 ctggaacaga ttgtaacccg tctggaaagt ggcgacctgc cgctggaaga ggcgctgaac
     6121 gagttcgaac gcggcgtgca gctggcacgt caggggcagg ccaaattaca acaagccgaa
     6181 cagcgcgtac aaattctgct gtctgacaat gaagacgcct ctctaacccc ttttacaccg
     6241 gacaatgagt aatggacttt ccgcagcaat tcgaagcctg cgttaagcag gccaaccagg
     6301 cgctgagccg ttttatcgcc ccactgccct ttcagaacac tcccgtggtc gaaaccatgc
     6361 agtatggcgc attattaggt ggtaagcgcc tgcgaccttt cctggtttat gccaccggtc
     6421 atatgtttgg cgttagcaca aacacgctgg acgcacccgc tgctgccgta gagtgtatcc
     6481 acgcttactc attaattcat gatgatttac cggcgatgga tgatgacgat ctgcgtcgcg
     6541 gtttgccgac ctgccatgtg aagtttggcg aagcaaacgc gattctcgct ggcgacgctt
     6601 tacaaacgct ggcgttctcg attctaagcg atgccaatat gccggaagtg tcggatcgcg
     6661 acagaatttc gatgatttct gaactggcga gcgccagcgg tattgccgga atgtgcggtg
     6721 gtcaggcatt agatttagac gcggaaggca aacacgtacc tctggacgcg cttgagcgta
     6781 ttcatcgtca taaaaccggc gcattgattc gcgccgccgt tcgccttggt gcattaagcg
     6841 ccggagataa agggcgtcgt gctctgccag tactcgacaa gtacgcagag agcatcggcc
     6901 ttgccttcca ggttcaggat gacatcctgg atgtggtagg agatactgca acgttgggaa
     6961 aacgccaggg tgccgaccag caacttggta aaagtaccta ccctgcactt ctgggtcttg
     7021 agcaagcccg gaagaaagcc cgggatctga tcgacgatgc ccgtcagtcg ctgaaacaac
     7081 tggctgaaca gtcactcgat acctcggcac tggaagcgct agcggactac atcatccagc
     7141 gtaataaata aacaataagt attaataggc ccctgatgag ttttgatatt gccaaatacc
     7201 cgaccctggc actggtcgac tccacccagg agttacgact gttgccgaaa gagagtttac
     7261 cgaaactctg cgacgaactg cgccgctatt tactcgacag cgtgagccgt tccagcgggc
     7321 acttcgcctc cgggctgggc acggtcgaac tgaccgtggc gctgcactat gtctacaaca
     7381 ccccatttga ccaattgatt tgggatgtgg gacatcaggc ttatccgcat aaaattttga
     7441 ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg cggtctgcac ccgttcccgt
     7501 ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca ttcatcaacc tccatcagtg
     7561 ccggaattgg tattgcggtt gctgccgaga aagaaggcaa aaatcgccgc accgtctgtg
     7621 tgattggcga cggcgcgatt accgctggca tggcgtttga agcgatgaat cacgcgggcg
     7681 atattcgtcc tgatatgctg gtggtcctca acgacaatga aatgtcgatt tccgaaaatg
     7741 ttggcgcgct taacaaccat ctggctcagc tgctttccgg taagctttac tcttcactgc
     7801 gcgaaggcgg gaaaaaagtt ttctctggcg tgccgcccat taaagagctg ctcaaacgta
     7861 ccgaagaaca tattaaaggc atggtggtgc ctggcacgtt gtttgaagag ctgggattta
     7921 actacatcgg cccggtggac ggtcacgatg tgctggggct tatcaccacg ctaaagaaca
     7981 tgcgcgacct gaaaggcccg cagttcctgc atatcatgac caaaaaaggt cgtggttatg
     8041 aaccggcaga aaaagacccg atcactttcc acgccgtgcc taaatttgat ccctccagcg
     8101 gttgtttgcc gaaaagtagc ggcggtttgc cgagctattc aaaaatcttt ggcgactggt
     8161 tgtgcgaaac ggcagcgaaa gacaacaagc tgatggcgat tactccggcg atgcgtgaag
     8221 gttccggcat ggtcgagttt tcacgtaaat tcccggatcg ttacttcgac gtggctattg
     8281 ccgagcaaca cgcagtgacc tttgccgccg gtctggcgat tggtggatac aaacccattg
     8341 tcgcgatcta ctccaccttc ctgcaacgcg cctatgatca ggtgctgcat gacgtggcaa
     8401 ttcaaaagct accggtcctg ttcgccattg accgcgcggg cattgttggt gctgacggtc
     8461 aaactcacca gggcgctttt gatctctctt acctgcgctg cataccagaa atggtcatta
     8521 tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta taccggctat cactataacg
     8581 atggcccgtc cgcggtgcgc tacccgcgcg gcaacgcggt tggcgtggaa ctgacgccgc
     8641 tggaaaaact accaattggc aaaggcattg tgaagcgtcg tggcgagaaa ctggtgatcc
     8701 ttaactttgg tacgctgatg ccagaagcgg ccaaagtcgc cgaatcgctg aacgctacgc
     8761 tggtcgatat gcgttttgtg aaaccacttg atgaagcgtt aattctggaa atggccgcca
     8821 gccatgaagc gctggtcacc gtagaagaaa acgccattat gggcggcgca ggtagcggag
     8881 tgaacgaagt gctgatggcc catcgtaaac cagtacccgt gctgaacatt ggcctgccgg
     8941 acttctttat tccgcaagga actcaggaag aaatgcgcgc cgaactcggc ctcgatgccg
     9001 ccggtatgga agccaaaatc aaggcctggc tggcataatc cctactccac tcctgctatg
     9061 cttaagaaat tattcatggc aggagtggca gcatgcaata caacccctta ggaaaaaccg
     9121 accttcgcgt ttcccgactt tgcctcggct gtatgacctt tggcgagcca gatcgcggta
     9181 atcacgcatg gacactgccg gaagagaaca gccgtcccat aattaaacgc gcgctggaag
     9241 gtggcataaa tttctttgat accgccaata gctattccga tggtagcagc gaagagatcg
     9301 ttggtcgcgc actgcgggat ttcgcccgtc gtgaagacgt agtcgttgcc accaaagtgt
     9361 tccatcgcgt tggtgattta ccggaaggat tatcccgtgc acaaattttg cgctctatca
     9421 acgacagcct gcgacgtctc ggcatggatt atgtcgatat cctgcaaatt catcgcgggg
     9481 attacaacac gccgatcgaa gagacgctgg aagccctgaa cgacgtggta aaagccggga
     9541 aagcgcgtta tatcggcgcg tcatccatgc acgcttcgca gtttgctcag gcgctggaac
     9601 tacaaaaaca gcactgctgg gcgcagtttg tcagtatgca ggatcactat aatctgattt
     9661 atcgcgaaga agagcgcgag atgctgcctc tgtgttatca ggagggcgtg gcggtgattc
     9721 cgtggagccc gatggcgcgg gggcgactga cgcgtccatg gggagaaact accgcacgac
     9781 tggtgtctga tgaggtgggg aaaaatctct ataaagaaag cgatgaaaat gacgcgcaga
     9841 tcgcagagcg gttaacgggc gtcagtgaag aactcggtgc aacacgagca caagttgcgc
     9901 tggcctggtt gttaagtaaa ccgggcattg ccgcaccgat tatcggaact tcgcgggaag
     9961 aacagcttga tgagctgctg aacgcggtgg atatcacttt gaagccagaa cagattgccg
    10021 agctggaaac gccgtataaa ccgcatgcgg tcgtaggatt taaataagag tccaggcctg
    10081 atgagacgtg acaagcgtca catcaggcat cggtgcacaa ctacgacaga atacccagcg
    10141 gccagtgatg accgataaag tacaggatgc ctgcggaaat caccccggcg acaatatcgt
    10201 cgatcatgat ccccatgccg ccatgtacat tgcgatcaaa ccagcggatc ggccacggct
    10261 tccacatatc cagaatacgg aaaatcacaa acccggcggc aacccactgc cagtcattgg
    10321 tcggcagcgc catgagcgtg atccacatac caataaattc gtcccagaca atgctgccgt
    10381 gatcgtgcac gcccatatct ttcgccgttt gatgacaaag atagacgccg atacagatcc
    10441 ccaacatcac caccagcgag tagagctgcc agggtaaaaa ggtcatcaga taccagaaag
    10501 ggatcgccgc cagcgatccc atcgtgccag gaacgattgg gcttaatcca cttccgaatc
    10561 cgacagcaag cagatgccac ggattactca tcttcaggcg acttttcgcg acatctttat
    10621 ggcgtggcaa aatggtcata tcctttccag tcaaacgtga caggttcgcc gtcacgaata
    10681 aaacaaagcc cttcgatatc ggcggtcatt tgcccgatac aggtaaacgg tacgccaagg
    10741 tgtccgagag caacatccag cgcgccacgg ttcagttccg gcaccgtgaa acacaactcg
    10801 taatcttcac cgccagagag cgcccagcgc agcgcctgtt ccggttcaac atggcgagaa
    10861 agcacatcag aaaacggcag caatgccagg tcaatacgtg cgccgcagtc gctggctttc
    10921 acgatatgcc cgagatcgga aatcagaccg tcagagagat cgatagctga atttgccaga
    10981 tcgcgcagtg cctgcccctg taaaatacgc ggcgatggac ggagatgacg tttgatcaag
    11041 tagtcagcat ctttagcatc ggcaacctgc aaacggtttt gcaaaatcgc cagcccggcg
    11101 gcgctatcgc ccggtgtacc ggtcacatag atccagtcac ccggtttcgc cccagagcgc
    11161 gttaaggctc gtcccatcgg aacaaagccg tggataccca acgtcattga taatggccca
    11221 cgcgtggtat cgccgccaat gagttgcata tcgtaataat tgagaagatc aaacaaactg
    11281 tcgctgaagg actcaagcca cgcttcgtct acgtccggta aggttaatgc cagcgtcagc
    11341 caggccggat cggcccccat cgctgccaga tcgcttaggt tcaccgccag tgctttataa
    11401 gccagatcag caggatcgat atcagggagg aaatggttgc ccgccaccag cgtatcagtg
    11461 ctgatcgcca gggtctgttt ctcggggata ttgagaagag cgcaatcgtc gccgatgccc
    11521 agttcgacat caagacgaga acttcttaca cggtcaaaat aacgggcaat cagggagaac
    11581 tcgccacatg ccatacgtta tgcctcagca ggtaaaaaag aaaaggccgg cgatcatgga
    11641 acagtcttcc gtgaatctac cggcctggat attacttttt gttagggcga atcacaggtg
    11701 ctgctttatc gagtacgccg ttgacgaact tatggctgtc ttctgcgccg aacgatttcg
    11761 ccagttcgat cgcttcgtta atggccactt tgtatggcac atcactacgt ttagacagtt
    11821 cgtacagcgc aatgcgcagt actgcttttt ctacctggcc cagttcttcc agcaggcggg
    11881 acaggtaggg cttcatcagt ccgtcgaggt atgcggtgtt agtcgccacc ccggccagca
    11941 gctcacggaa gtacagaacg tcaacgtctt ttacatcctg ttcagccagg aactggtatt
    12001 caacatcagc gatgtcgttc tgggacaact gccaggagta gagcgcctgg acggcacact
    12061 cacgagcgcg gcgacgagca gcaggtttca cggatttccc cttactaatt tcaggccttg
    12121 atggctttca atacattaat catttcaagc gcggtcagtg cagcttctgc acctttgttg
    12181 ccagctttgg tgccagcacg ttcgatcgct tgttcaatgc tttcagtggt cagaacccca
    12241 aaagcaaccg gaatttcgct gtcctgggca acatgcgcca ggccgttgct tgcaccacca
    12301 gcgacatatt caaagtgggc agagccacca cgaataaccg tacccagcgc aatcaccgcg
    12361 tcgtatttac cggttttagc cagtgcaccc gccgccagcg gcagctcata ggcaccaggc
    12421 acccaaacaa cggtaatgtt ttcatctttt acctgaccga tacgtttcag tgcgtcaatt
    12481 gcaccttcca gcaggctgtc attgataaag ttgttgaaac gcgcgatggt gatggcgacg
    12541 cgagcgtccg gggtagcaac gttagcttca ataatgttca ttttcttcct tcgggttcga
    12601 gtatggcccc gcaggggggc ggattttagc ataatatttc gtgcgctgct tccctttcga
    12661 gccgggagat catgccccca ctaaatgcag gcaaacatcc gggcctacat gacgtatctc
    12721 tttgaattta aattgggggg cgtcggctaa tttctcaagc cctggcagcg agcataatcc
    12781 acgggcgtcg ctgcctaata gtttaggtgc gatatagaca atcagttcat cgactaaacc
    12841 cgcctgtagc aatacgccag cgagcgttgg ccccgcttcc acccagatgc tgttaatctg
    12901 ctgtttaccc agttgcatca tcagtacaac cagatccaga tgacctttat gctctggaat
    12961 cagcaaggta cgcaccgttt ccggccactc acgagaatct tcctgcgtac gcgcgaacca
    13021 ggtttcgccg ggctgctgca caatgcgatg ttccggcgtc acgcgatttt ggctatcaat
    13081 cacaatacgt actggctggc ggagattttg ttgcgggtag agcgcctgag tttgttcatc
    13141 cagttcagac caacgcactg ttaaggctgg atcatccgcc agcaccgtgg cgctgctggt
    13201 taaaatggca tgactttgtg cgcgctgtcg ttgtacgtcg cgccgcgcct gaggcgaagt
    13261 gatccactgg ctttcgccgc tcgccatcgc cgtgcggcca tcaagcgatg cgccaagttt
    13321 taactgaata taaggaaagc cggtgcgcat ccgcttgaga aagcctttat tcaattgctc
    13381 ggcttcactc atcatcagcc cgtggctgac gtcaatgcca gcctgttgca gacggtaaag
    13441 tccacgccca gcgacctgcg ggttaggatc ttgcatcgct gcgaccacgc gcgctacgcc
    13501 agcggcgatg agtgcgtcac agcacggtgg cgtacggcca tgatggctac agggttcgag
    13561 tgtcacatac gccgttgcgc ctttggcttt ttcacccgcc atacgcaacg cgtgtacttc
    13621 ggcatgtggt tcacccgctc gttggtggta accttcaccg acaatttcgc catctttgac
    13681 aatgacgcac ccgacattcg ggttggggtg ggtggtaaaa cgtcctcgtt gcgccagctt
    13741 tagcgcccgc gccatgtaat actcgtcctg cacggcttag tcctccaggc gcgcgatctc
    13801 ttcgccaaat tctttgatat cttcgaaact gcggtagaca gaggcaaaac ggatataggc
    13861 gactttatcg agctttttca attgctccat caccagattg ccaatcatct tgctcggtac
    13921 ttcgcgctca ccggtggcgc gcagctgcga tttaatatga ttgattgcca tttcaacgta
    13981 atcggaactc accggacgtt tttccagcgc ccgcagcatt ccgctacgca atttctcttc
    14041 attaaacggt tcacgcacgt cgttgctttt tacaacacgc ggcataacca gctccgccac
    14101 ttcaaaggtg gtgaaacgtt cattacacac cagacactgc cgacggcggc gtacggatga
    14161 accctcgccc acgagacgag agtcaattac cttagtgtcc acggcgaaac agaatgggca
    14221 atgcatacgg cgtcctgacc aggtggttaa cggaaatcta ttttaccctg aactgacgat
    14281 acaacaaagg cgcaacgctt ttgagataaa ggatcgatgc tttcgctgct tttaccctct
    14341 accattaggc taattgcgac gagaaaggga cgacctatga atactaacgt ttttcgactg
    14401 ctcctgctgg gaagcctgtt cagccttagc gcctgtgtgc agcaaagtga agtgcgacag
    14461 atgaaacaca gcgtcagcac gctgaaccag gagatgacgc aactcaatca agaaacagtc
    14521 aaaatcactc agcaaaacag gctgaatgca aaatccagca gtggggttta ccttctgcct
    14581 ggagcgaaaa caccggcaag actggaaagc cagatcggta ctttacgtat gtcgctggtg
    14641 aatattacgc ctgatacaga tggcaccacc cttacactac gtattcaggg tgagtcgaat
    14701 gatcccttgc ccgcattcag cggaactgtt gaatatgggc aaattcaggg aacaatagac
    14761 aactttcagg aaatcaatgt gcagaatcaa ttgattaatg cccctgccag cgtcctcgcc
    14821 cccagcgatg ttgatattcc gttacaatta aaggggattt ctgtggatca gttaggcttt
    14881 gtacgtatcc acgacattca acctgttatg cagtaaacgt attttgggac gattttgtgc
    14941 gtcccgcaac atctttcccc gtcattttgt tactctgctt acatcacttg gattgatagt
    15001 aaaagtttgc aacaagggcg aaagtcagta caatccccgc ccgaatgtgt gtaaacgtga
    15061 acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg tgagcaatga
    15121 tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc gcctttttca
    15181 ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac tggcagccgg
    15241 tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg acaaaccgca
    15301 gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc acacccgttt
    15361 cggaccgcag atccgcaacg atacctacct tgagtacgaa gcattcgcta aaaaagactg
    15421 gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact ccgatgctaa
    15481 aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt tctccatcga
    15541 caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact tcgcgaacaa
    15601 ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt acatgggtct
    15661 gggtaccgat atcgacactg gcctgccgat gagcctgtcc atgaacgtct atgcgaaata
    15721 ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc gtttcaaaat
    15781 taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca tcggcttcac
    15841 caacttcgac tggggttccg atttagggga tgacagcggt aacgcaatca acggtattaa
    15901 gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact acgatcactg
    15961 gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg acgatgcaga
    16021 actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg gttacctggt
    16081 agtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat ttctgattgt
    16141 taacaacatg tgctttgttc atgccggatg cggcgtaaac gctttgtccg gcccacaaaa
    16201 gcacgtaaat tcaatatatt gctgaaataa cgtaggcctg ataagcgtag cgcatcaggc
    16261 aattttacgt tttaatcact tcttcttgtt catcatcgct ttcagatcag cgaatgggtt
    16321 gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg tcgtaccata
    16381 ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac acaacaattc
    16441 ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt gaacggtaag
    16501 ttcacgcagg ttagaataaa caaactcgcg ggaacagcga ccgcagaccc acggatagat
    16561 ttttaatgct ttttcgcgat agccgctttc taaccgtgca tagttttttg ggatgatagc
    16621 catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga tgcggggatt
    16681 ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt tcaacatcaa
    16741 cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc cactttctgc
    16801 tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc tacgtagatg
    16861 gaagatgcag tgccgatgga aacaccgata agcatggtca gcgagaagcc ttccagtacc
    16921 ggaccaccga agagatacag catcaggata accatcaagg tagtaccgga tgtgatcaag
    16981 gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt accgcgacgg
    17041 atcttgcgga agttttcacg aattcggtcc gataccacga tactgtcgtt aagcgagtaa
    17101 ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg gaataacgac
    17161 aaaataccca gcgtaatgat aacgtcgtgc gccagcgcaa taaccacccc tgccgccagt
    17221 cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc catcaacgcc
    17281 atcgcaccgg tttgcgcaag gtctgccccc acgctcggac cgacgaactc aatacgcttc
    17341 actgctgcat tctgattggt ggattcgtta atcaccttca gaacttggct gcccagcacc
    17401 tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg gctgctacca
    17461 aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg cattacgtca
    17521 atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa atccagcccc
    17581 cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag accagagatg
    17641 ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg gtttagttgt
    17701 tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct tgacgcgctt
    17761 gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa acatcgacgt
    17821 cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac ccactgcgta
    17881 caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac tgaatgcgcc
    17941 acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact cttctttaat
    18001 acgttcgttg atcagtacgt tcgcatcgac cgctaccgca agggttaaga cgatacccgc
    18061 gatacctggc atactcagtg ttgcgcctgg caacagcgac ataataccga cgatcaagat
    18121 caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga agatgatcat
    18181 gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgct caatgttctg
    18241 catacccagg gtcgggccaa ttgtacgttc ttcaacaatc tgaatcggcg cgatcaacgc
    18301 accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga tgccggtgat
    18361 acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct cttcctgttt
    18421 caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact ccacaaacag
    18481 ggttgccatc ggtttgccga tgttgtcctt ggtgaagtta gacataatgt tgccaccagc
    18541 gctatcgagc gagatgttaa cctgcggctg gttgtattcg tcctggctgg aagtggagtc
    18601 ggtgatatgg tcaccagtca gaattacgcg tttgtacagc acaaccggct gaccttcgcg
    18661 ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg cctgatcaac
    18721 attggtgttt accagacgga attccagcgt tgccgtcgcg cccagaatct ctttcgcacg
    18781 cgcagtgtcc tgaataccag gcagttcaac aacgatacgg tcagcaccct gacgctgaac
    18841 caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa tgttctgctg
    18901 caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac gcagctggtt
    18961 gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg caatggcttc
    19021 atcacgagct ttagcatcgc ggaaagtgat gctcaggccg tagttgtttt ctttacgaac
    19081 agtggtatac gggatgccct tttcgcgcag gtcactgcgt aggctatcga tattttgttc
    19141 ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa cgccgccacg
    19201 caggtcaagg ccgagcttca tcggctcagc atgaatagct gccagccagc gcggcgttgc
    19261 cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta atgcttcacg
    19321 agcgcgcaac tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct cttccagtgc
    19381 cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga tcagcgtttg
    19441 ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct caccaaacag
    19501 gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga cgtacttcca
    19561 caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt acagcgcctt
    19621 catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta cttcagtggt
    19681 gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc gaccaaccag
    19741 gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct ttttgtgttc
    19801 tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac cgaataccac
    19861 cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg ccgctaccgc
    19921 atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca acgttcaaag
    19981 gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct aatttaccct
    20041 cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg ttatgaatgg
    20101 tgttgagtcg cgcgcctaat atttcgttgc aacggtcgag atgatgcaag taagcgcgtg
    20161 aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta tcgctcttat
    20221 atttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca tttcgggcgt
    20281 tgcgggttgg cattacgcag tcaaacatat cgataccgcg acgtacgcct tcaaccaggt
    20341 cttccggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt tgcggacaca
    20401 catgctccag aatacggtgc atatctgctt tcggctcacc cacagccaga ccgccgacag
    20461 cgtagccatc aaagccgata tctaccagac ctttaacaga tatatcacgt aaatcttcgt
    20521 aaatactgcc ctgaattata ccaaataacg catttttgtt accaagactg tcaaaacgct
    20581 cacggctacg cttcgcccaa cgcagagaca tctccataga gcgttttgcg taatcccagt
    20641 cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca agatcgtact
    20701 gaatctccat cgacttttcc gggtcgagga aaatcgggtc gccgttgatc gggttacgga
    20761 agtgaacgcc ctgttcggtg attttacgaa tatcaccaag gctgaagacc tggaagccgc
    20821 cggagtcggt aagaatcggt cctttccact gcataaaatc gtgcatatcg ccgtgcagtt
    20881 tcatgatttc ctggcccggg cgcagccaca ggtggaaagt gttgccgagg ataatttgcg
    20941 cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag gtgccaacag
    21001 gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca cggcgtgcgc
    21061 gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag aaaaacagtc
    21121 caacgtttaa accagcgccg cggaattact ccccgacgcg ctcattaatt gcctgcggat
    21181 tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg atatttctct tctaccgccg
    21241 ctttataggc attcatggtg tgttgataac cggcaaaggc cgaaaccagc ataatcagcg
    21301 tcgactctgg caagtggaag ttcgtcacca gcgcatcgac cactttgtac tggaagcccg
    21361 gatagataaa gatttgggta tcgtcaaaga acggttcgat aagatcgttt tttgccgcct
    21421 gtgccgcgct ttccagcgaa cgtactgaag tggtgccaac cgcaatcacc cggttaccgc
    21481 gcgctttcgc cgccagtacc gcgtctacca catcctgcgg tacttcagcg tattccgagt
    21541 gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg gaaggtgccc gcaccaacgt
    21601 gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa tttttccagc aaaggctcgt
    21661 caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg tttttcgcta taaacggttt
    21721 gataaagttc gcggtcagcg tcttcgtccg gacgatcgat atacggcggc agcggcatat
    21781 ggccgatgct gttgaggata tccagtaccg agcgttcatc attaaattcg acttcaaaca
    21841 gtgcgccgtg gcgcgcggtc attgttgcgt tgatactttc gtcatcgccc agcaacagtt
    21901 ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc aagaatgcgt ttgtcgtcga
    21961 gcatccgctc aaccagcact tcaatcttac cgccgctggc tttacgccca aacagacgcg
    22021 ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc ggggttgagc ttatcgagta
    22081 aatcggtgaa agtaccgtgc gtcagcgcac ccgtcggccc gtccagcgac agtaaacgac
    22141 agctactgcg ttcaggcatg ggatagtggg caatcaggga ttcgggcaat tcaaaggaga
    22201 aatcggtaac gcgcatgaca ctgactcaga ctaaaataag agtcgggtag tctagtgccg
    22261 gggcgctctc cctgcaacct ttacccccct ttggatagaa tataatcgat gaatttttta
    22321 gctcacctgc atttagccca tctcgcggaa agctcgcttt ctggcaattt actggctgat
    22381 ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg tcgtggctgg cattcatatg
    22441 catcgacgta ttgactgaca atctgccgga agtccgcgaa gcacgggagt ggtttcgtag
    22501 tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact ttctttcccg
    22561 ccactggtcg cagctgtcgc cggactttcc actacaggaa tttgtctgtt atgcccgcga
    22621 gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctga acaattactt
    22681 gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga acgtgttaaa
    22741 cggcatggca agccgccgcc cacgtctgga tgccctgcgt gactcctggt acgatttaga
    22801 cgctcattat gacgccctcg aaacccgctt ctggcagttt tatccgcgga tgatggcgca
    22861 ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga aatggcaggc
    22921 aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca ttgcacggca
    22981 ttgagaaacg gtgacgcggg tagcaccact tcactggcct cgccacggtt gattgccacc
    23041 agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc cgcatacagc
    23101 acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat cattcgccgg
    23161 tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg tttacggcaa
    23221 gacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca cggtacacca
    23281 ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg accgagcagc
    23341 gttttaaatc gcgcagtatc gtggctatcg agctgattaa acatacgtaa ttgttgttga
    23401 tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc gtcaatttgc
    23461 tgcggatcat aagagatgtc ggtattggca agaaatcccc acaacgggaa tgtgaagcca
    23521 cgatagttca tggcagcatc ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa
    23581 tgttcgccga caatatacgc ttccgactgg gtttctttcg ccgcttcggt gatcccggca
    23641 acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac cacatccagc
    23701 cgccagccgt ccatattcca cggcgctttc agccagtggc ggacaatact gtcttccccg
    23761 cgataaattt cattcaccag actttccgac tgataatcca gcttcggcaa gctggcatag
    23821 ccaagccagt cgagcgccgt gccatcatca ctaaacaagt accagtcgcg ccagggcgat
    23881 tcagagttgt gacaagctcc ccccgtgcca agattgtgcc tgtcaa
//
LOCUS       DAJTXY010000054        23684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTXY010000054 DAJTXY010000000
VERSION     DAJTXY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.054"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="OO566_002227"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="OO566_002227"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6605504.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="OO566_002228"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="OO566_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6605505.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="OO566_002229"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="OO566_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6605506.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="OO566_002230"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="OO566_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6605507.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3874..3998
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3880..3924
                     /gene="pheM"
                     /locus_tag="OO566_002231"
     CDS             3880..3924
                     /gene="pheM"
                     /locus_tag="OO566_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6605508.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="OO566_002232"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="OO566_002232"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6605509.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="OO566_002233"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="OO566_002233"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6605510.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRIAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="OO566_002234"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="OO566_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6605511.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="OO566_002235"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="OO566_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6605512.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="OO566_002236"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="OO566_002236"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6605513.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="OO566_002237"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="OO566_002237"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6605514.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="OO566_002238"
     CDS             10418..10882
                     /locus_tag="OO566_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6605515.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="OO566_002239"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="OO566_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13298
                     /locus_tag="OO566_002240"
     CDS             11895..13298
                     /locus_tag="OO566_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6605516.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPD"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="OO566_002241"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="OO566_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6605517.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="OO566_002242"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="OO566_002242"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6605518.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="OO566_002243"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="OO566_002243"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6605519.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="OO566_002244"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="OO566_002244"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6605520.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="OO566_002245"
                     /pseudo
     CDS             complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="OO566_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="OO566_002246"
     CDS             complement(20164..20457)
                     /locus_tag="OO566_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6605521.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="OO566_002247"
     CDS             complement(20454..21743)
                     /locus_tag="OO566_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6605522.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFIYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21799..22737)
                     /locus_tag="OO566_002248"
     CDS             complement(21799..22737)
                     /locus_tag="OO566_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6605523.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIISLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTQGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22757..23521)
                     /locus_tag="OO566_002249"
     CDS             complement(22757..23521)
                     /locus_tag="OO566_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6605524.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5843 a         5779 c         6240 g         5822 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agtatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatagcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtgac
    11401 tacattatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcaacaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgagg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaaccaata cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagataa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 cccctgcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggaaatga tcgccagcgg
    22321 gctgttgatc ttttcctgag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat
    22381 gtgaccatcg acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac
    22441 acttaaccgt gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg
    22501 gtgtttatct ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc
    22561 gtcgttttgc tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg
    22621 gtcggtattt tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag
    22681 ttcagcataa cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt
    22741 ttcttctccg taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg
    22801 cctccggcga atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa
    22861 tgccgaccag ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact
    22921 gatttaccgg ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt
    22981 taatatcgga ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat
    23041 cttcaagcgt gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat
    23101 taatcaccgg aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt
    23161 cgccggaacc ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca
    23221 gcgcttttgc ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca
    23281 ggctgtgcgg cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc
    23341 cgccaacggt cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa
    23401 tggcattgag atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt
    23461 ctggagtgac aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca
    23521 tgatttctcc tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa
    23581 aatagtgtgc gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa
    23641 tcttgcaaaa aaaatgcgat tgctaaaaca aaagtgcaat ttgc
//
LOCUS       DAJTXY010000055        23499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTXY010000055 DAJTXY010000000
VERSION     DAJTXY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.055"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(34..1398)
                     /gene="tdcG"
                     /locus_tag="OO566_002250"
     CDS             complement(34..1398)
                     /gene="tdcG"
                     /locus_tag="OO566_002250"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6605525.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALHRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1470..1859)
                     /locus_tag="OO566_002251"
     CDS             complement(1470..1859)
                     /locus_tag="OO566_002251"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6605526.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1873..4166)
                     /gene="tdcE"
                     /locus_tag="OO566_002252"
                     /pseudo
     CDS             complement(1873..4166)
                     /gene="tdcE"
                     /locus_tag="OO566_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(4200..5408)
                     /gene="tdcD"
                     /locus_tag="OO566_002253"
     CDS             complement(4200..5408)
                     /gene="tdcD"
                     /locus_tag="OO566_002253"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6605527.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5434..6765)
                     /gene="tdcC"
                     /locus_tag="OO566_002254"
     CDS             complement(5434..6765)
                     /gene="tdcC"
                     /locus_tag="OO566_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6605528.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMAGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6787..7776)
                     /gene="tdcB"
                     /locus_tag="OO566_002255"
     CDS             complement(6787..7776)
                     /gene="tdcB"
                     /locus_tag="OO566_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6605529.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAILGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7875..8813)
                     /gene="tdcA"
                     /locus_tag="OO566_002256"
     CDS             complement(7875..8813)
                     /gene="tdcA"
                     /locus_tag="OO566_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6605530.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            9003..9347
                     /gene="tdcR"
                     /locus_tag="OO566_002257"
     CDS             9003..9347
                     /gene="tdcR"
                     /locus_tag="OO566_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6605531.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            9603..10142
                     /locus_tag="OO566_002258"
     CDS             9603..10142
                     /locus_tag="OO566_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605532.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            10164..11340
                     /locus_tag="OO566_002259"
                     /pseudo
     CDS             10164..11340
                     /locus_tag="OO566_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
     gene            complement(11950..12326)
                     /gene="rnpB"
                     /locus_tag="OO566_002260"
     ncRNA           complement(11950..12326)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO566_002260"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(12359..13510)
                     /gene="garK"
                     /locus_tag="OO566_002261"
     CDS             complement(12359..13510)
                     /gene="garK"
                     /locus_tag="OO566_002261"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6605533.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHTWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(13607..14497)
                     /gene="garR"
                     /locus_tag="OO566_002262"
     CDS             complement(13607..14497)
                     /gene="garR"
                     /locus_tag="OO566_002262"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6605534.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(14527..15297)
                     /gene="garL"
                     /locus_tag="OO566_002263"
     CDS             complement(14527..15297)
                     /gene="garL"
                     /locus_tag="OO566_002263"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR6605535.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(15313..16647)
                     /gene="garP"
                     /locus_tag="OO566_002264"
     CDS             complement(15313..16647)
                     /gene="garP"
                     /locus_tag="OO566_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6605536.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            17022..18593
                     /gene="garD"
                     /locus_tag="OO566_002265"
     CDS             17022..18593
                     /gene="garD"
                     /locus_tag="OO566_002265"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6605537.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(18645..19469)
                     /gene="agaR"
                     /locus_tag="OO566_002266"
     CDS             complement(18645..19469)
                     /gene="agaR"
                     /locus_tag="OO566_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6605538.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            19718..20518
                     /locus_tag="OO566_002267"
                     /pseudo
     CDS             19718..20518
                     /locus_tag="OO566_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
     gene            20518..20982
                     /gene="agaV"
                     /locus_tag="OO566_002268"
     CDS             20518..20982
                     /gene="agaV"
                     /locus_tag="OO566_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6605539.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            20993..21772
                     /locus_tag="OO566_002269"
     CDS             20993..21772
                     /locus_tag="OO566_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6605540.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            21762..22571
                     /locus_tag="OO566_002270"
     CDS             21762..22571
                     /locus_tag="OO566_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6605541.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            22658..23092
                     /locus_tag="OO566_002271"
     CDS             22658..23092
                     /locus_tag="OO566_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6605542.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            23089..>23499
                     /locus_tag="OO566_002272"
     CDS             23089..>23499
                     /locus_tag="OO566_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6605543.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAH"
BASE COUNT         6122 a         5993 c         5545 g         5839 t
ORIGIN      
        1 ttgtgcaccc aaggatgaaa gctgacagca atgtcagccg cagaccactt taatggccag
       61 tcctccgcgt gatgtttcgc ggtatttatc gttcatatct ttgccggttt catacatcgt
      121 ctcgatcact ttatcgagtg aaacacgcgg tgccgaggtg cggcgcatcg ccatccgcgc
      181 ggcgtttact gctttcacgg cattaatggc attacgttca atgcacggga tttgtacctg
      241 tccggcaacc ggatcgcagg tcagcccaag gttatgctcc atcgcgattt ccgccgcatt
      301 gcatacctgc gccggactgc cgcccagtag ttcagttaac cctgccgctg ccattgaaca
      361 ggccacgcca atctccccct gacagccgac ctccgcgcca gagatggagg cgttcatttt
      421 atacagcgcg ccaatagccc ccgctgccag aaaatagcgg gcaattgacc gctcgtttac
      481 cggacgacgg aacttatcgt aataagccag cactgccgga ataatgccgc acgcaccgtt
      541 agtcggtgcc gttaccacgc gcccgccagc tgcgttttct tcactaaccg ccagcgcgta
      601 catgttgatc cagtcgatga cattcatcgg atcgttagag atgttatcgc tagaaaccag
      661 ctgacgatgc agcgctacgg cacgacgcgg cacattgagc ggaccaggca gcacgccttc
      721 agtgttcatc ccgcgttcaa taccgtcatg catcacttgc cagatacggg caaaccctgc
      781 gtcaatttcc gctttgctgc gcagcgccag ctcgttgtgc atcatcaggc cagatataga
      841 caggccgttg taatcacaca ttttcagcag ttcacctgct gagtggaaat cgtaaggtac
      901 ggacgtttcg acatcgtgcg acaggccgaa gtgttcttct tcgacaataa acccgccgcc
      961 gacagagtaa taggttttac ttaatagcgc ttcctgtccc ttccaggcag tgatccgcat
     1021 tccgttctca tggcgaggca acatttcggg atggaagata atgttctttg ctacaggaaa
     1081 atcaacaata tgcgcaccag atgccactgg cagccgcccg ctgcgcgtta ctaactctat
     1141 aaatgcaggg atctcatcaa tgacaacatc ctgcggactg tttcctgcca gtcccatgat
     1201 aatggcgaca tccgtggcat ggcctttgcc cgtcagtgac aacgacccgt acagatcgac
     1261 cacaatatgg ctcgtcgcgg ttaataagcc gctactttcc agccgatcaa taaaactttt
     1321 tccggcattc attggcccca cggtatgcga actggaggga ccaatcccaa ttttgaaaat
     1381 atcgaatgca ctaatcatat ccacaccctc ggattgccgt tcagtgaagt ggagcggaac
     1441 gaccttgcga ccgtcccgct cacgaggctt tacgcactac gtactgcgat agcttcaatt
     1501 tccagcttca catctttcgg caaacgcgca acctgcacac agctccgggt cgggtaggtc
     1561 gcctgatgct catcgaagaa ctgcttatag acttcattga tggtggcaaa atcattcaga
     1621 tcggtgatga acacggtcat cttgatgata tcgcccacgc tcagcccggc agcaaccacg
     1681 atcgctttga cgttttcgag gcttaaacgc gcctgatctt gcacatcagc cgggatctca
     1741 ccggtctgcg ggcaaaccgg aatttgcccg gaggtgaaga ccatgctgcc taaatcaacg
     1801 ccctgaacat aagggccgat tgcgcctagg gcacgttgcg tttcgataat ctttttcata
     1861 catcctccgg cgtcagagcg cctgggtaaa agtacgtgaa ataacatcct gttgctgttc
     1921 acgggtcagt gcgttgaagc gcacggcgta gccagagaca cggattgtca ggttaggata
     1981 tttttccggg tgctcgatgg catccagcag catttcccga ttcattacgt tgacgttgag
     2041 gtgttgaccg ccttcgacat ccgcttcgtg gtggaaatac ccatccagca ggccgacaag
     2101 gttggtttta cgtactggat cttctttgcc cagcgccgca ggaacgattg agaaggtgta
     2161 cgagatccca tctttggcgt aggtgaacgg cagtttcgcc accgacgtca atgaggccac
     2221 ggcacctttg cggtcacgac catgcatcgg gttagcgccc ggcgcgaacg gtgttccggc
     2281 gcgacgaccg tccggcgtat taccggtttt ctggccgtac accacgttag aagtgatagt
     2341 cagaatcgac tgggtaggga cggcgttgcg ataggttggc agcgctttaa ttttcttcat
     2401 aaagcgttca accaggtcgc aggcaatgct gtctacgcgc tcgtcgttgt tgccgtactg
     2461 cggatattca ccgtcgattt caaagtccac cgccaggccg ttttcgtcac ggattggttt
     2521 cacgcgggca tatttgatgg cagacaggga gtccgtcgcc accgacaggc ccgcgatgcc
     2581 gcatgccata gtgcgataga catcacgatc gtgcagcgcc atcagcgaag cttcgtagct
     2641 gtacttgtcg tgcatgtagt gaatgatatt cagcgcgctg atgtactgca ccgccagcca
     2701 gtccatgaag tgatcgaggc tgtccatcac tttgtcgtaa tccagcacgt cgtccatcag
     2761 cggtgctgtt ttcggcccga cctgaatctt cagcttctcg tccaccccgc cgttaattgc
     2821 gtagagcagc gttttcgcca ggttagcgcg tgcaccaaag aactgcattt gcttaccaat
     2881 caccattggg ctgacgcagc aggcaatcgc gtaatcgtcg ctgttgaagt cagtacgcat
     2941 cagatcgtca ttttcatact gcaaggaaga ggtgacgatc gacacctgcg cggcatattt
     3001 tttgaaggca atcggtaatt cttccgacca aagaatggtc aggttaggtt ccggtgccgg
     3061 ccccatagtg tgcaggtgtg caaatagcgg aaggagtttt tggtcaccag cgtacgaccg
     3121 tccagcccca tcccgccgat cacttccgtc gcccagattg ggtcgccgga gaacagcgaa
     3181 tcaaattccg gtgtacgcag gaagcgtacc atacggatct tcatgatgaa gtgatcgatc
     3241 agttcctgtg cctgctgctc attgaggacg ccagctttaa agtcgcgctc aatgtagata
     3301 tccaggaacg atgccgtgcg ccccagcgac atcgcgccgc cattttgcga tttcactgcc
     3361 gccagataag cgaagtagag ccactgcacc gcttcctgtg cattctgcgc cgggcgagag
     3421 atatcaaagc catatttcgc cgccatttcc tgaatctgca acagcgcatg acgatgctct
     3481 gccagctcct cacgcagacg gatggtggct tccagatcct cgcctttttc cagacgagac
     3541 tggagatcgg caaattgcag ttcgcgttca cgcaccagat aactgatgcc atacagcgct
     3601 acgcggcgat agtcaccgat aatgcgccca cgaccatagc catctggtaa accggtcagc
     3661 acgccagatt tacggcagcg cagcatatcc ggtgagtaaa catcaaatac gccctggtta
     3721 tgggttttac gcagatcggt aaacagatat tcaaattcac tgtccatttc acggccatag
     3781 gcgtggaatg aacttttaat catattaatg ccaccgaacg ggtgtagcgc acgtttcaac
     3841 ggcgcatccg tttgcaggcc aacaattttt tccagcggct ggttaatata tcccgcatca
     3901 tgagcggtaa ttgtggtggc aatattggta tcgaaatcaa ccggcgcgtg ggttgcgttt
     3961 tcgatacgga tgccttccat tactttttct cacaattccg tggtggcagg cgtcgcttcg
     4021 gcgagaaaag attcatcgcc ttcatacggt gtatagttat gttgaataaa atcgcggaca
     4081 ttaatttcgc ttttccagtc cgtaccttta aagccaagcc atgcgtcagc gtacagctta
     4141 tcgctggtat caatatctac cttcatgaaa aataatctct ctacaatact tcaactaaat
     4201 tatgcaaatt ctgcgggcgc gttaacttta cctaaatgaa tggcatccaa agcaatcatt
     4261 ttttcttcgt tagtcggaat aacgacgcaa atgacatgcg cattttcact ggaaacaatt
     4321 cgctcgccaa aagagttaga tcgattattc atttctgtat caatctctac gcctaataca
     4381 gccaaatgtt ccatgaccag acgacgaatt aaacttgaat tctctcctat tccgccggtg
     4441 aatataattc catccagccg acgtaatgaa gctgcatgtc cggcaatatg acgggcaatt
     4501 cggtgaacaa aggttttaat tgccagttgc gcgcgttcgt gaccttcatg ccaggctttt
     4561 tccagaacac gtaaatccga agaaagaccg gaaataccta ataatcccga ctctttattc
     4621 actacgcgtt ccaggtcacc caggctctgg ttggtttggc tggcgaccca ggacatcgca
     4681 ccaaagtcga catcgccact gcgggtaccc atcatcaagc cttccagcgg cgtcattccc
     4741 attgaggtgt caacactctg accgttgcga actgcgcaga ttgacgcgcc attgccaaga
     4801 tgcgccacaa ccaggccgga gtcatcttcc gccagattca gcagcgaatg tgcgcgctgg
     4861 gaaacatagc ggtgcgacgt gccgtggaaa ccataacggc gtacacctaa ctcttcataa
     4921 tatttccacg gcaggccgta taaataagct tccggagcca tcgtctggtg gaaactggta
     4981 tcaaatacgg ccacctgagt tacgcccgga aataattgct gcgccgattc aataccactt
     5041 aaatttgcgt aattatgcag gggtgccagt ggagaaacgc gacggatatt atcaatgact
     5101 tcatcggtaa taatggcgga ctcggtaaaa atactgccgc cgtgagcgat gcggtggcca
     5161 attaaggcca cgctgtcatt taaattccgt ttttccagtt caaatgcaat tgccttcaat
     5221 gcaccttcgt agctgtggag agccagcggt gctggctctc ccccatttac ggataagaat
     5281 gcattttccg agttaatacc gtcggcaata cctgacatta atacttcaca gtcgctggca
     5341 tcgagtacgg aaaacttaat cgaagacgaa ccacagttaa taaccaaaac aaccggaaat
     5401 tcattcatct cttttctcat cctgagttac ggattaaaac agtttgtata cgatgttcag
     5461 gatggtcagc agaccaatca cggtaacaaa cacgttatcc agacgaccac ggtatttcgc
     5521 cagagacggc gctttacgga tagcatacat cggcaacagg cacagcaggg atgcgataat
     5581 cggtgcgccc atggcttcaa tcaggtcgag gatgttcggg ttggcgtagg caacaaccca
     5641 ggtggagccc atgatgaaga tcatgctgag agtattcagt ttacccagcg acactttggt
     5701 tttgtcacct ttataaccga acttcagaat cagaccattc aagccttcca gcgtccccag
     5761 atagtgaccg aagaaagatt tgaagatagc cacgagtgcg atgatggaag ccgcatattc
     5821 cagtgtaatc gcgaacgttg ttttcgtacc ggccatggac gcaaagtggt tagccagata
     5881 agaaagcact ggaatattct gcactttggc ttccgccatg ttggccggag acagagtaaa
     5941 caggcagcta aaggcaaaga acatcaccac tgcaaccatc agcatgctgg cacgagaaat
     6001 gatttgggaa catttacgtt cggtgaagtc gcgaccgaag tctttctcat actcttcacg
     6061 tttagaaacc acgaaggaag agacgattgg cgagaagtta aaggagaaaa ccatgatgga
     6121 aatccccagc cagacagtga tcaggatacc gtcatgaccg gttaacgaca gcgaaccgag
     6181 gtcaacctgg tcgataactg cagagttcca gtaagggatc agcgacagag aaatcagcac
     6241 caggctggcg ataaacggcc ataccaggta gctcatcact ttaaccatca gatccttacc
     6301 aaaccagatg acgaaagcca tcagcagcaa caggaacagc gccacaaagc cgcgattcag
     6361 cggtgcaaat ccgagctggt tttcccagaa cgtcataaag gtattggtaa tagtaacgcc
     6421 ataaatccac agcagtgggc aaatcgcgaa gaagtacagg aacgtgataa ccacgccgcc
     6481 agttttacca aaatgctctt ccaccgtttc cgtaatgttg ccggaagggt tagagccaga
     6541 aagacacaga cgcgccagcg cccggtggca ataaaatgcg atggggtatg ccaataccaa
     6601 catcagaaga atcgggatca gtccgccaaa acctgcgcgg atagggaaga acagcacccc
     6661 ggcgccgatt gccgtaccaa acaagcctaa cgtccatgtg gtatctgatt tacgccagga
     6721 cgattgtttt gtctggctgg atacaatgct atctgaagta ctcatatcct atcctcaacg
     6781 aattaattaa gcgtcaacaa aaccggtgat ttgagagacg cgagaaagat cgatattgcc
     6841 gccggaaata atactgacgg tttttctgtt ttgaatatat tggtctaatt taccgcttaa
     6901 taatgcagca catgccagag cgcctgcgcc ttcggtgacg actttattgc gctgaattaa
     6961 ggcaatcata ctgtttctga tttcgtcttc gctgaccagc acgatgtcat cgactaattc
     7021 acgaacgatt tcgtaagtta aattacccgg gcgggagaca tcacaaccat ccgccagggt
     7081 gccggtagtt cggtgcgtgg ttatttctcc ggagtggaaa gaagccgcca tgccgtgaac
     7141 gttttctgac tgtacgccaa taacacgaat ggtcgggtta atagatttaa ttgccaccgc
     7201 aataccagca attaaaccac caccaccaat tggcacaatc acgttatcga catcatagag
     7261 atcttccata atttccagac caatcgttcc ctggccagca atcactttcg gatcatcgta
     7321 aggtgggata aaaatacgac cttccatttc gacaatttcg ctcactttag cgatagtgtc
     7381 gttgaagtta tcaccatgca gaacgacttc tgcggagtag tcgcacgttg ccgctacttt
     7441 ggattttggc gcaccttttg gcatcaccac tttaccgtcg atacccagta tcgcgcagga
     7501 gagcgaaacc ccttgcgcat ggttgcccgc agaacaggcc accacgcctt tgcgtttttc
     7561 cgcatcggtc agtgaactta atttattaaa tgcgccacga attttaaatg aacccgtacg
     7621 ctgcatattt tcaaacttca ggaatatttc acctttgcaa cgttcactaa aatagttgga
     7681 gcgaggcatg cctgttttat aaattcgccc agccagtcgt tgtttcgctt caataatatc
     7741 atcaatagca accggcagat cgtatgtaat atgcattata aaacctcttc gcctgaatta
     7801 aatatgtagg taaccgtaac cgacacctgc aaaacagaca ggtggattat ttatgtttaa
     7861 ataaaaacaa ataactaacc aacttctatt aattgccttc gtctacaccc attataagat
     7921 gaatactctt tggctaattc caccaaaacc gatgctgctt ttttaatacg ataatttttc
     7981 gaccataccg cggcatattg tgccacaggt aatgtttctt caaccggaat agtaataaat
     8041 tgattagaac caaaaggtga cgtcatatca caaggaatta cagttaagaa atcagcattg
     8101 agaacaagat tataaattgt cacgactgag tcggttttaa cgatgttttc aatactgatg
     8161 ccatttcttt gtaacgtagt aagcagttcg ctgtagtacc ccatattagt ttgtggcaac
     8221 acccactgtt cgttcttcaa cgactccagc gtggtggtgc cggtgcatgt tcgggactta
     8281 ctggctacca gcacaaactc ggactcgaac agcggctcaa catgtaaatc ctgaagcttc
     8341 atttctgcac ttaacgtacc aatcgcaaaa tccagtcgac cgtcgcggat tgccggtaag
     8401 aacgaagaca gttgcgcttc atacatagaa acctgcgctt tcgggaacac ctctttgaac
     8461 ttgttgatca tccctgacat aaaagtaaaa ccaatcaatg aaggaaaacc aaatgagact
     8521 tccaccaccg cctcagaaga cataccgctt atctcattaa ccatattttt catttcacgg
     8581 gtaatggatt cggaacggga gagtaacaat tgaccggcag gtgttaatgt tacaccggta
     8641 tttttccgca ccactaattc cacaccaaaa taatcttcaa tatcgttaat gattttactg
     8701 acggccggtt gagttaaccc taattctttt gcagccgagc cgatagagcc acttctaatg
     8761 acttcctgaa agactaccag gtgctgcgtt ttcggaagaa gaatagtgct cataacgacc
     8821 tacgttaatt acctcattga cggcatgaag tgtatcaaaa tgaaataaac aggatatgtg
     8881 cgaccactca caaattaact ttcattactt tccaaggtat cgttattaca attaaatacc
     8941 tttaaatatc aacaagttaa agcataaaaa tcagcataaa ccctgatttc tatcaaaaaa
     9001 aaatgacggg gataaccata ttttatggcg ataacatcat tcgttatgtg gttaatacaa
     9061 aaaaggggct gagaccatat ttcaagcaat taccggataa ttaccaggca aaatttgagt
     9121 taaatttaat gagcaagttc tcaaatttta ttataaataa accattttca gcgataaata
     9181 ctgcggcacg tcacattttt tcacgctatt tgttggagaa caaacattta ttttatcaat
     9241 attttaaaat ttcgaataca ggtattgatc atctcgaaca attgattaac gtcaactttt
     9301 tctcttctga caggacgtca ttttgtgaat gcaatcgttt tccataaatt cttctcccct
     9361 cataggcgac gaatagcatt ttgtgttgag gatcacaaaa cgaataattg ctggtcgccg
     9421 cgataaggtc agacgaagtc aacaaggggg attttcactg tgctttacat cagcgtagga
     9481 cacagaatgt tgcttcgttt actgtaacgc cgggtaaatg agcgtttttt gatagtgcgt
     9541 aagtgtcgtg cgaggaaaat aagcatctat tattgtttgt catgacgaca aggcaggcat
     9601 caatgaaagg cttcccaatt gcacatattt ttcacccttc aatcccgcca atgcacgcag
     9661 tggttaacaa tcacaataga aatattgatt attggacggt aaaaagaaag tttgcagaaa
     9721 ttgtctccac caatgacgtt aataaaattt acagtataag taatgaactg cggagagtat
     9781 tatctgcaat tactgcattg aatttctatc aaggcgatgt tccttctgtc atgatccgaa
     9841 tccaaccgga aaatatgagt ccattcatca tagatatttc tacaggagaa catgatgatt
     9901 atatcataca aacattagat gtaggcactt ttgcaccttt tggtgaacaa tgtacttgct
     9961 cagccgtcaa taaaaaagag ctggaatgta ttaaggagac gatttctaag tattgcgcaa
    10021 agttcaccca aaaagaagcc atcttaacac cccctaggca ctttaataaa acctgcatta
    10081 cttcagattg ttggcaaatt ctcttttttt cacccgatca ttttaataat gatttttatt
    10141 gattatttat aaaggataaa ataatgttcc ctgtttcatc aattggtaat gatattagta
    10201 gtgatttagt ccgtcgaaaa atgaatgacc ttcctgaaag ccccatagga aataacctcg
    10261 aagcactagc acccggtata gaaaaactaa aacagacctc tattcaaatg gtcactttac
    10321 ttaacgcgtt acaaccaagt ggaaaatgca ttatcactgg tgattttcaa aaagaattag
    10381 cgtacttaca aaatgtaatt ctttataatg actcgtctct tcgtctggat tttttaggtt
    10441 ataacgccaa aattattcaa cgatcggaca atacctgcga acttaccatt aatgaaccgt
    10501 taaaaaacca ggaaatatct acaggcaata tcaatgttaa ttgcccatta aaagatattt
    10561 acaatgaaat caggaggttg aacgtaatct ttagttgtgg gactggagat atagttgatc
    10621 tatcctctct ggacttacgt aatgtcgatt tagattatta tgatttcaca gataaacata
    10681 tggctaatac tattctaaat cattttaaat tggattcaac aaattttact aatgccaaca
    10741 tgtttcaggt taattttgtt agttcaacac aaaacgccac aatctcctgg gattatttac
    10801 taaaaataac gcctgtttta ataagcatta gcgatatgta ttctgaagaa aaaatcaagt
    10861 ttgtcgaaag ttgtttaaat gagcttggag acattaccga agaacaatta aaaattatga
    10921 gatttgcaat tataaaatct ataccaaggg caactcttac agataaatta gaaaatgaat
    10981 taacaaaaga aatatataaa agctcatcga aaatcaacaa ttgcttgaac agaattaaat
    11041 taccagagat gaaagaattc tcatcaaaaa aatatatgat tacatcgata caatcattga
    11101 agattatgaa aatatcaaag aaaatgctta tctggtcgtc ccccaaatta attatactat
    11161 ggatttaaac atagaagact ctagctcaga agagttaata ccctcgagaa agacgaaaat
    11221 tctccggaca atggctttga ggtcggggaa tataacacat atgaagcata taactcagag
    11281 aatcaatatt ttaccagaga ggactatacg tataattacg accttttaaa tgcaatatag
    11341 acttaataac ttcttacaaa gtctattatt aataccgtag atattcatct taccctgtgc
    11401 accaaaagca aaaacttatc gacgcgggtt tgaaactgat ggcaaccgca aaaatgcctg
    11461 atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg cgtgcttttg
    11521 taggcaggat aaggcgttca cgcagcatcc ggcatgaata aagcgcactc aacaatctga
    11581 aacccgccgg aacggtttat tacgtacatc aggtgaaact gaccgataag ccgctttctt
    11641 ttgggtatag tgtcgtggac agtcattcat ctttctgccc ctccaaaagc aaaaacccgc
    11701 cgaagcgggt ttttacgtat atcaggtgaa actgaccgat aagccgcttt cttttgggta
    11761 tagcgtcgtg gacagtcatt catctttctg cccctccaaa agcaaaaacc cgccgaagcg
    11821 ggtttttacg tacatcaggt gaaactgacc aataagccgc tttcttttgg gtatagcgtc
    11881 gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa gcgggttttt
    11941 acgtaaaaca ggtgaaactg accgataagc cgggttctgt cgtggacagt cattcatcta
    12001 ggccagcaat cgctcactgg ctcaagcagc ctacccgggt tcagtacggg ccgtacctta
    12061 tgaaccccta tttggccttg ctccgggtgg agtttaccgt gccacggact gttaccagcc
    12121 gcgcggtgcg ctcttaccgc accctttcac ccttacctga tcccgcttgc acgggccatc
    12181 ggcggtttgc tctctgttgc actggtcgtg ggtttccccc ccaggcgtta cctggcaccc
    12241 tgccctatgg agcccggact ttcctcccct ccgcccgtct cccccgaaga ggacgacgac
    12301 aaagcggcga ctgtctggtc agcttcggcg cgcagtatag agggtttgcg cgcccttgtc
    12361 accccgtgtt gcgcattcca atcgccagtg tcgcggcaat attacgtgaa gcacggcaga
    12421 tattgtcata agccccgcgg aatgcttcgt ccaacgtacc tatgctggtc aatacgctga
    12481 agaccgcatc aatgccatgc tgatgtacaa cgccaacatc atcggtcagg ctacccgcac
    12541 ccgcaatgcc aataaccggt ttatggtact tcttcgccac gtttgcgacg ccaatcggta
    12601 ccttgccgtg aatactctgg ctgtcaatac gcccttcacc ggtgatcacc agcgtacaat
    12661 cgtgaatatg ttcctccaga ttcagcgccg tagtgacgat ttcaatacca cttttcagtt
    12721 ccgcaccaag aaacgccatc agcgccgcac ccataccgcc cgcagctcct gcgccgggga
    12781 catcttttac atcaacatgc agcgcttttt taatgacatc ggcatagtga gagaggttat
    12841 tgtccagctc aacaatcatc gcttcactgg ctcccttttg tgggccaaag atgcgcgatg
    12901 cgccgttatc gcccaccagc ggattggtga catcacaagc gacgcgaatg acgcaatctt
    12961 ttaagcgcgg atcgaggccg gaaatatcaa tatcattcag agtattaaga ctaccgccgc
    13021 caaaaccaat ttcattgccg ttggcgtcgc ataatttcgc ccccagcgcc tgtaccatgc
    13081 ctgcgccgcc atcatttgta gcgctaccgc caatgccgat aataatgttt gtcgcgccgc
    13141 tctccagcgc ctgcaggatt aactcgcctg tgccgcgtga agtggtcacg agtggatcgc
    13201 gtttttccgc aggtaccagc tccagcccac tggccgccgc catttcaata aacgcggttt
    13261 tgccatcgcc ggagatcccc caactggcat tcactttctc gcccagcggc cctgtaaccc
    13321 aggtgtgacg ttcagaaccc tgggtggctg caatcatcgc ttccaccgtt ccttcgccac
    13381 cgtcggcaac cggaatagaa acgtactgtg catcaggaaa aatttcccga aatccttttt
    13441 ctatcgcctg cgcaacctcg ctggcagata aactttcttt ataagagtct ggggcgatta
    13501 cgattttcat acctatgcct gttaccacat gacgccggag ggcgtttctc ttattcggcc
    13561 tggattccag gcccggattg caatacgcca tccgggcacg acgtcattaa cgagtaactt
    13621 cgactttcgc cagtttttcg tagtagcacg ccagggcgct atgatccgcc gttcctaaac
    13681 catctgctcg cagtgcctgc atcatctcca taaccgcagc tgtgagcggc agttgcgcgc
    13741 cgacgccgtg ggaagtatcc agcgcatttg ccagatcctt aatatgcaga tcaatacgga
    13801 agcccggctt gaagttgcgg tccatcacca tcggcgcttt ggcatccagc acggtactgc
    13861 ccgccagtcc accgcgaatt gcctgataca ccaggtccgg gttaacgccc gctttagttg
    13921 ccagcgttaa cgcttctgac atcgcggcaa tattcagcgc cacaatgacc tgattcgcca
    13981 gtttggtgac gttacctgca ccaattgccc cggtatgcac cacggaaccc gccattgctt
    14041 tcatcaaatc atagtatttg tcgaaaatag ccttgtcgcc gcccaccatc actgacagcg
    14101 taccgtcgat ggctttcggt tcaccgccgc tcaccggagc atccagcata tcaatgcctt
    14161 tcgctttcag cgcttcgctg atttcacggc ttgccagcgg tgcgatagaa ctcatatcga
    14221 tcaataccgt acctggcttc gcgccttcaa taatgccatt ctcacccagc gccacctctt
    14281 tcacatgagg ggagtttggc agcatggtta tgatgacgtc gcactgttca gcgatcgctt
    14341 tagccgtaga ggctgtttct gcacctgcag caatcacgtc agcaatagct tctgggttac
    14401 ggtcagcaac caccagcgag taacctgctt tcagaaggtt tttactcatt ggtttaccca
    14461 taatccctag gccaataaaa ccaactttca tagtcatatc aatcatctct cttgttgcgg
    14521 tggtggttat tttttaaagg tatcagccag tttatgagtg gcagagcgga agacgccgag
    14581 atcgctgccg acagccacaa acgtcgcgcc ccattccaga taacgacgcg catcggcttc
    14641 gaccggcgcg aggataccgc tgggtttgcc gtgcgcgctg gcacggttaa aaatgtgctg
    14701 aattgctttt tgtacatccg ggtgtgatgc attgccgaga tggcctaatg ccgcggccag
    14761 atcgctgggg ccgacgaaga tgccgtctac gccttcggta gcggcaatgg catcgacgtt
    14821 atctacgccc tgctgacttt ctatctggac cagaatagtg atgttcttgt tcgactgagc
    14881 gaaataatcc gccacggtgc caaacatatt ggcgcggtga gaaacggaga cgccgcgaat
    14941 gccttccggt gggtaacggg ttgatgccac cgcctgctct gcttcctctt ttgtttctac
    15001 aaaaggaatc aggaagttat agaaaccgat atccagaaga cgcttaataa ttaccggctc
    15061 gttggtcggc actcgcacta ctggcgcgct ggcgctgcct ttcaaggcca ttaactgcgg
    15121 aataaacgtg gagatatcgt ttggcgcatg ttcgccatcc agcaccagcc agtcaaaccc
    15181 agccaaacca agaacttcag tgctaatcgg gttagagagt gctgaccagc aaccaatttg
    15241 tacctgtttc gcagccagtg cggctttgaa tttattcggg aaaacatcgt tattcatcgc
    15301 ttataccttt gcttatttct gcaattccat acgtttaatg tcgccaacta cgaagaggta
    15361 gcagaccatc gccatcagcg ctgaacatcc cacgaaaacc agtgctgcat tgaaggagtg
    15421 cagttcactt accaggtagc caatcaccag tggagtgaca atggaggcaa cattgccaaa
    15481 gacgttaaag acgccgccac agaggccaac aatctctttc ggcgcggtgt cagaaatcac
    15541 cggccagccc agagcaccaa atcctttgcc aaaaaaagcc agcgccatca gcatgaccac
    15601 cagcgtggtg ttgttggtgt agttacataa gatgatggtg gaagccagca acattcccag
    15661 cacaatcggt agcttacgtg ccagggtcag ggataaaccg cgtttgatca gataatccga
    15721 gaagacacct cccagcacgc cgcccgcaaa accacacagt gccggaatcg aggcgaccag
    15781 acccactttc agaatcgaca tgcctttttc ctgcaccaga taaatcggga accaggtgag
    15841 gaagaaccag gtgatggtgt tgataaaata ttgtccgaaa aatacgccca acatcatgcg
    15901 gttagagagc aattgcttga tgtaatgcag tttgggaccg cttgctgccg cgctgcccgg
    15961 ctttttgtgg tccatatcga ccaccgcgcc attttcagag ataaacttca gctcttcctc
    16021 agacatacgt gggtgatctg tcgggttatg aatcaacttg atccacagcg ccgtcagcac
    16081 aaaaccaatc acccccataa cggtaaagac gtgctcccat ccccaggcga aagtcagcca
    16141 gccaagcaga ggcgaaaaga gcgccagcga gaaatattgt gccgagttaa agatggcgga
    16201 ggcagtacca cgttctttcg tcgggaacca ggcggcgaca atacgggcgt tcgccgggaa
    16261 tgatggagct tccgagaatc ccagcataaa gcgcataaag aacatggaga tccctgccca
    16321 ggccagcggg aacatatcaa caaagccttg caggaaggtg aacagcgacc agaaaaagag
    16381 gctgtaggtg taaacttttt tcgagccaaa cttatcaagc aaccagccgc cggggatttg
    16441 catcagcaag taggcccagc caaaagcgga gaagatgtaa cccatcgaaa ccgcacttaa
    16501 ctgcaactct tttgccactt cggtaccagc aatagacagc gttgcacgat cggcgtagtt
    16561 aacggcggta acaataaaaa taatcagtaa tattaaatag cgggtatgca cacctttcct
    16621 tttttcgtca acggtgtcca gaatcatttt atttacctcg ggtacttatg ctgattttta
    16681 ttattatggg gaaggtgtta tttataagtt tcatttatgc cgtaacgaca atgaactcgg
    16741 gaattagtat aagcagcgcg agaataataa tcattgtgca aatgctaatt taattaatac
    16801 tttttaaata ttattttgag catatgcaca taaggttgcg cgctaaagca cagatttgcg
    16861 ctttaccttg ccgggcggca ctgcaatccc tgaaatgatt gacattgatc acatttctgc
    16921 gtttaaactc ctgacattct tatttcaccc aatgaagtca tttattttta aatgagacca
    16981 ggtcctcatt ttaataaccc ctggctggag aatattgcac aatggccaac atcgaaatca
    17041 gacaagaaac gccaactgcg ttttatataa aagttcacga cacagataat gtggcaatta
    17101 ttgttaatga taatggcctg aaagcaggaa cgcgttttcc ggatgggctg gaattaattg
    17161 aacatattcc ccaggggcat aaagtcgcat tgctggacat tccggctaat ggtgaaatta
    17221 ttcgttatgg cgaagtgatt ggttacgccg tgcgtgcaat cccacgcgga agctggatcg
    17281 acgaatcaat ggttgtacta ccggaagcgc cgccgttaca cacgctgcca ctggcaacca
    17341 aagtcccgga acccttaccg ccgctggaag gatacacctt tgagggctat cgcaatgccg
    17401 atggcagcgt gggcaccaaa aacctgctcg gcatcaccac cagcgtccac tgtgtggcag
    17461 gcgtggtgga ctatgtagta aaaatcattg aacgcgatct gctaccgaaa tacccgaacg
    17521 tcgatggcgt ggtggggctg aatcatttgt acggttgtgg cgtggcgatt aacgcaccgg
    17581 cggcagttgt acctattcgt accattcaca atatttcgct gaaccctaac tttggcggcg
    17641 aagtaatggt gattggcctg ggttgtgaaa agttgcagcc tgagcgcctg ctgactggaa
    17701 cggatgatgt gcaagctatt ccagtagaaa gcgccagcat tgtcagtttg caggatgaaa
    17761 agcatgtcgg ttttcagtcc atggtcgagg atattttgca ggtcgccgaa cgccatctac
    17821 aaaaactgaa tcaacgtcag cgagaaacct gtccggcttc agaactggtt gtcggcatgc
    17881 agtgcggtgg cagcgatgcg ttttctggcg taacggcaaa cccggcggtt ggctatgcgt
    17941 ctgatctact ggtgcgctgc ggcgcaacgg tgatgttttc agaagtcacg gaagtgcgtg
    18001 acgcgatcca tctgctgaca ccacgcgcag tgaacgagga ggtcggcaaa cggctgctgg
    18061 aggagatgga gtggtacgat aactatctca atatgggaaa aaccgaccgc agcgccaacc
    18121 cttcgccggg caacaagaaa ggcggtctgg caaacgtggt agagaaagcg ctcggctcca
    18181 ttgctaaatc gggcaaaagt gcaattgttg aagtgctgtc gcccggtcaa cgcccgacta
    18241 aacgcggatt aatttacgcc gcgacgccag ccagcgattt tgtctgtggc acgcaacagg
    18301 tggcttcggg tatcaccgtg caagtgttta cgaccggtcg tggtacgccg tacggcctga
    18361 tggcggtacc cgtcattaaa atggcaaccc gcaccgagct ggcgaaccgc tggtttgatt
    18421 taatggatat taatgcgggc accatcgcta ccggcgaaga aactattgaa gaggtgggct
    18481 ggaagttgtt ccactttatt ctcgacgtcg ccagcgggaa gaagaaaacc ttctcggatc
    18541 aatggggact gcataaccag ctggcggtgt ttaacccagc accggtgacc tgataattca
    18601 attcgtatta tgcccgatag cacacgttta tcgggcatat tgagtcacag cgaagacgcg
    18661 tgatccccga ccagaatcac ttcaacccca gcctttcgca gtccttccag actatccgca
    18721 ggaatgcctt catcaacaat gatcatgtcg atacgttgag tatcaatgat cttatgtaaa
    18781 ctggaacgat tgaatttact ggaatcggtg accacgatga tccgttccgc aacttcgcac
    18841 atccgacggt ttaaacgggc ttcatcttca ttatgtgtgc tgacgccgcg ctccagatcg
    18901 atcgcatcta caccaagaaa cagcatatcg aagtggtaat tttgcagcga ttgctcagcc
    18961 tgatcgccgt aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat cagcagctca
    19021 acgccttccg cttccagcaa cgcattagcc acgttcatac cgttggtcat cgcaattacg
    19081 tcagtgtact tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga atcgaggatc
    19141 acccgatgac ctggctgaat caactcaacg gcagctttcg caacgctgcg tttcatcgcg
    19201 gtgttcagtg cgcttttatc ttccactgat ggctcgactg acggcgtcgt gctatcgcag
    19261 atcaacgcgc caccataggc acgcacagcg atcccctgct tttccagaaa agccagatca
    19321 ttgcggatcg tcacagtaga tacgccatac aatgccgaca gatcgttaac ctgcacactc
    19381 ccttgctgtc gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc tgtcactcgc
    19441 ttctcacctg aagcgtcggt attactcata gtaagtcctt tcgtaaaact ttcgtttcat
    19501 ttcgttttgc ctattaacgc ctttctatta agcaaatgca agcccatctt gcccattggc
    19561 gcaagctact ctcgtttcag tgactttcat tatgtttctt ttgtgaatca gatcagaaaa
    19621 ctattatatt tcgttttatt tttatctcac catgatgcag tattaattga aacaaaacga
    19681 aagattaata tcgcagtaat ctgaactgga gaggaaagtg aaacatctga cagaaatggt
    19741 gagacagcac aaagcgggca aaacaaatgc aatttatgcc gtttgttccg cacatccgct
    19801 ggtgctggag gctgcaatcc gctacgccag tgcaaaccaa acgccgttac tgattgaagc
    19861 aacctccaat caggtagacc agttcggcgg ttataccgga atgacgcccg ccgattttcg
    19921 cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg caggatgcgc tgattctggg
    19981 tggtgaccat ctggggccaa accgctggca aaacctgcca gccgctcagg caatggccaa
    20041 tgccgatgat ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc accttgattg
    20101 cagcatgtcc tgtcaggacg atccaattcc cttaactgat gacatcgtgg ctgaacgcgc
    20161 cgcccgtctg gcgaaagtgg cggaagaaac ctgtcgcgaa cactttggcg aagccgatct
    20221 ggagtatgtc attggtaccg aagtgccggt acctggcggc gcgcatgaaa ccttaagcga
    20281 gctggcggtc accacgccgg atgccgcccg cgccacgctg gaagcccatc gtcacgcttt
    20341 tgaaaagcaa ggtttgaatg ccatctggcc acgcatcatt gccctggtgg ttcaacccgg
    20401 cgtcgaattc gatcacacca acgttattga ttatcagccc gccaaagcga gcgccttaag
    20461 ccagatggtc gaaaactacg aaacgctgat tttcgaagcg cactctaccg attatcaatt
    20521 gttttaagcc ggattgatga acgcttgatt cacggtcagg tcggcgttca gtgggtcgga
    20581 tttgcggggg caaatctggt gctggtagcc aacgatgagg ttgccgaaga tccggtacaa
    20641 caaaacctga tggaaatggt actggcagaa gggatcgccg tacgtttctg gacgctgcaa
    20701 aaagttatcg acaacattca tcgcgccgcc gatcgacaga aaatcctgct ggtttgtaaa
    20761 acacccgccg atttcctgac gctggtgaaa ggtggcgttc cggtgaatcg cattaacgtt
    20821 ggcaatatgc actacgccaa tggcaaacaa caaatcgcca aaacggtttc tgtggatgcg
    20881 ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg tggaatgctt cgttcagggc
    20941 gtcccgacag agcctgctgt ggacctcttt aaattacttt gagggattca tcatggaaat
    21001 cagcctgttg caggcatttg cgttgggcat tatcgccttt atcgctggcc tagatatgtt
    21061 taacggctta acccatatgc accgcccggt ggtcctcggc ccgttggtcg ggctggtact
    21121 tggcgatctg cataccggaa ttttaaccgg cggtacgctg gaactagtgt ggatggggct
    21181 ggccccgctg gcgggcgcac agccgcctaa cgtgattatc ggtactatcg tcggcacggc
    21241 gtttgccatt actactggcg tgaaacccga tgtcgcggtc ggtgtcgccg tacctttcgc
    21301 tgtcgcagta cagatgggga ttaccttcct gttctcggtg atgtctggcg tgatgtctcg
    21361 ctgcgaccgg atggcggaga acgccgatac tcgcggcatt gaacgcgtga actatctggc
    21421 actgctggca ctcggcactt tctattttct ctgcgctttc ctgcctatct acttcggcac
    21481 ggaacatgca aaaaccatca ttgatgtcct gccgcaacga ttaatcgacg gcctcggcgt
    21541 cgcgggcggc atcatgccag caatcggttt tgccgtgctg ctgaaaatca tgatgaaaaa
    21601 cgtctacata ccctacttca tcctgggctt tgttgccgca gcctggctca agttaccggt
    21661 gctggctatt gctgccgccg cgctggcgat ggcgctgatc gatctgctgc gtaaatctcc
    21721 tgaaccgact caacctgcgg cacagaaaga ggaatttgaa gatggcatct aatcaaacca
    21781 ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac tggcgtcaat gaaaacgtgt
    21841 atgaagatcg gagcattgcc gcggagctga cgaaaaaaga tatcaaccgc gtcgcctggc
    21901 gttccatgct gttacaagcg tcttttaact acgaacgtat gcaggcttct ggctggttgt
    21961 acggtctgct gcctgctctg aaaaagatcc acactaataa acgcgacctg gcgcgcgcca
    22021 tgaaggggca tatgggtttc ttcaataccc atccgtttct ggtgacgttt gttatcggca
    22081 ttatccttgc gatggagcgt tctaagcagg acgttaacag tattcagagc accaaaattg
    22141 ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt ctggctaacg ctactgccga
    22201 tttgtggcgg gataggcgca agcctcgcgc tacaaggctc tattcttggc gcagtcgtct
    22261 ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt tggtctggcg cattatgctt
    22321 accgcatggg cgtggcggcg attcccctta ttaaagcgaa taccaaaaaa gtcggccatg
    22381 ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt ggcaacctat gttcgtttaa
    22441 gcaccacgct ggaaatcacg gcgggcgacg cagtggttaa gttacaggct gatgttatcg
    22501 acaaactgat gcctgccttc ttaccgctgg tctacaccct gaccatgttc tggctggtac
    22561 gccgcggcta gagtccgctg cgcctgattg cggttaccgt ggttctaggg atcgtcggta
    22621 aattctgcca tttcctttaa atacaatgag gtattagatg ttaagtatta ttttgacagg
    22681 gcatggcggg tttgccagtg gcatggaaaa agcaatgaag caaatcctcg gcgatcaatc
    22741 gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc gcgctgctta catcgcagct
    22801 tgaagaggcc atcgcccaac tggattgtga agacggcatt gtttttctga cggatttgct
    22861 gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg caaaaaccgg gctgtgaagt
    22921 gattaccggc accaatttgc aactgctact ggagatggtg ctggagcgcg aagggttaag
    22981 cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac cgagggctga ccagtctggt
    23041 ggacgagtta ggccgctgcc atgaagaata tccggtcgag gaaggaatat gacacacgtt
    23101 ctgcgcgcca gaaggctgct gactgaagag ggatggttcg atgaccatca gttgcgtgtt
    23161 gctgacggtg tcatcgcagc aatcgaaccg attccagtgg gcgtgactga acgcgatgcg
    23221 gaactgctct gccccgctta catcgacacc catgtacacg gtggtgcggg cgttgatgta
    23281 atggatgacg cgccggatgt actcgacaag ctggcaatgc acaaggcacg cgaaggtgtc
    23341 ggcagttggt taccgaccac cgtaaccgcg ccgcttaata ccattcatgc ggcgctgaaa
    23401 cgtattgctc aacgttgcca acgcggcgga cctggtgcgc aagtgctggg gagttatctc
    23461 gaaggaccgt acttcacgcc gcagaataaa ggcgcgcat
//
LOCUS       DAJTXY010000056        23219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTXY010000056 DAJTXY010000000
VERSION     DAJTXY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.056"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="OO566_002273"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="OO566_002273"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6605544.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="OO566_002274"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="OO566_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6605545.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OO566_002275"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OO566_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6605546.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OO566_002276"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OO566_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6605547.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OO566_002277"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OO566_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6605548.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="OO566_002278"
     CDS             5281..5739
                     /locus_tag="OO566_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6605549.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="OO566_002279"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="OO566_002279"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6605550.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="OO566_002280"
     tRNA            6791..6875
                     /locus_tag="OO566_002280"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="OO566_002281"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="OO566_002281"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OO566_002282"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="OO566_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6605551.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OO566_002283"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="OO566_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6605552.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OO566_002284"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="OO566_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6605553.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="OO566_002285"
     CDS             complement(10156..13899)
                     /locus_tag="OO566_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6605554.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OO566_002286"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="OO566_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6605555.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="OO566_002287"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="OO566_002287"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6605556.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="OO566_002288"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="OO566_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6605557.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVIFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OO566_002289"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="OO566_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6605558.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="OO566_002290"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="OO566_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6605559.1"
                     /translation="MKKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OO566_002291"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="OO566_002291"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6605560.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OO566_002292"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="OO566_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6605561.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="OO566_002293"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="OO566_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6605562.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5820 a         6058 c         5588 g         5753 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgtt tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taatgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgcc tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 atagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaagc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacgaagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacggtaa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgaccggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattg
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgctga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat gaccggattt gcgcatactg aatggttggg attgaccgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggtctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggac gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcgc aacgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct ccccgataag ggagcaggct agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact ttcaaaagcc ccggaattct
     6901 caaacgaatc tgcaatcaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttgaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgtttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca gcaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacatac gacgtttcag caaaagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcaacatacc gaagaagtga ccgacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actggacctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 agccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaaca gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagcgcacg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaattccgtt gctgcccagc tcgcccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccatcgc cattttataa accggcgact
     9181 gctgtgcagc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacgtcca gctgacgctg gtagagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacggac
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt atcaaactca ccgcccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgataat
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcaaagattt
     9901 tacccagcag catggctcgg ttacccatgc gcggttgcag tttgccattg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tggcccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagtgc accgttgctg ttgaagactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttt gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccgttaccg attttctcca tcagcgggcc gatagaggta aagcgttcgt aagttgccgg
    11221 ataatcgcgc tctacgacca taatgtgcgg tgcagttttg cctgggatca ggtcgcattc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgctttta gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaagaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagttacag cataacatgc tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcaaatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taacactaat
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcggcatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaagtaat cactacgcag
    14341 gcgatataga aaatcaaaaa tactttcatt gcacctactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatcg cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgcc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gatgaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa accgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtta tgacagctcc agtagccctt
    15661 tcggaggcag atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgga gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt agaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccggctat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatatatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aaggggttta
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gcgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttga ctcgagcagc agaacgagac ggacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgcg ctgatgcgtg cacaaaaccg
    16381 agaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgtattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagc catcgcgatt ttacccaacg
    16621 cgcaaacatc agcgggcgca acgaaatggc gatgcttgga actgcgttga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc ggtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaactgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actggtggat accctggttg aacaacttac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggaag cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgtct ggtgccttca catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaggtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tgatattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggctatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc acagacaatc gccagtgatt gcttgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgca tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tgtcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg tacaccaaag cggtaattaa gattcagccc gaggttattt
    18961 tgattttcgc cactttcccc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc aaagttaaac
    19081 aaatcaacac gatcaccaaa atactgttct aggctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgagc tgtcaggtcg tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgataa tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtcctg caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccattatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg attttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgctacaa gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aatgtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgaaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctctgattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgttta
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccttaaca
    20461 caccatccgg ataattctcc gccagtagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcat
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcatgatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcatcagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccagaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagtagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtttt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccaggcc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc ctgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aacgccagct cgctggagac attgctcgac agcatgaatg cccctgttgc
    22561 ctttactggc ttcctggtgg cactgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg ttcagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aacaatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 gcagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggagagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaa
//
LOCUS       DAJTXY010000057        23154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTXY010000057 DAJTXY010000000
VERSION     DAJTXY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.057"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..391
                     /locus_tag="OO566_002294"
     CDS             <1..391
                     /locus_tag="OO566_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6605563.1"
                     /translation="MSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLW
                     SGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGH
                     AVLACGEMVQSGRSLKQEPTEMIQATA"
     gene            complement(435..5052)
                     /locus_tag="OO566_002295"
                     /pseudo
     CDS             complement(435..5052)
                     /locus_tag="OO566_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(5145..5792)
                     /gene="rnt"
                     /locus_tag="OO566_002296"
     CDS             complement(5145..5792)
                     /gene="rnt"
                     /locus_tag="OO566_002296"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6605564.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5895..6302)
                     /gene="gloA"
                     /locus_tag="OO566_002297"
     CDS             complement(5895..6302)
                     /gene="gloA"
                     /locus_tag="OO566_002297"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6605565.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6383..7480)
                     /gene="nemA"
                     /locus_tag="OO566_002298"
     CDS             complement(6383..7480)
                     /gene="nemA"
                     /locus_tag="OO566_002298"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6605566.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7517..8116)
                     /gene="nemR"
                     /locus_tag="OO566_002299"
     CDS             complement(7517..8116)
                     /gene="nemR"
                     /locus_tag="OO566_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6605567.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8219..8458
                     /locus_tag="OO566_002300"
     CDS             8219..8458
                     /locus_tag="OO566_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6605568.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8507..9403
                     /locus_tag="OO566_002301"
     CDS             8507..9403
                     /locus_tag="OO566_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6605569.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9484..9813
                     /locus_tag="OO566_002302"
                     /pseudo
     CDS             9484..9813
                     /locus_tag="OO566_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
     gene            complement(9784..10167)
                     /locus_tag="OO566_002303"
                     /pseudo
     CDS             complement(9784..10167)
                     /locus_tag="OO566_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(10160..10951)
                     /locus_tag="OO566_002304"
                     /pseudo
     CDS             complement(10160..10951)
                     /locus_tag="OO566_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(10954..11190)
                     /locus_tag="OO566_002305"
     CDS             complement(10954..11190)
                     /locus_tag="OO566_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6605570.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            11391..11825
                     /gene="slyA"
                     /locus_tag="OO566_002306"
     CDS             11391..11825
                     /gene="slyA"
                     /locus_tag="OO566_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6605571.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(11872..12339)
                     /gene="slyB"
                     /locus_tag="OO566_002307"
     CDS             complement(11872..12339)
                     /gene="slyB"
                     /locus_tag="OO566_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6605572.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            12613..13722
                     /gene="anmK"
                     /locus_tag="OO566_002308"
     CDS             12613..13722
                     /gene="anmK"
                     /locus_tag="OO566_002308"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6605573.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            13820..14149
                     /gene="mliC"
                     /locus_tag="OO566_002309"
     CDS             13820..14149
                     /gene="mliC"
                     /locus_tag="OO566_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6605574.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            14208..14864
                     /gene="pdxH"
                     /locus_tag="OO566_002310"
     CDS             14208..14864
                     /gene="pdxH"
                     /locus_tag="OO566_002310"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6605575.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            14993..16267
                     /gene="tyrS"
                     /locus_tag="OO566_002311"
     CDS             14993..16267
                     /gene="tyrS"
                     /locus_tag="OO566_002311"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6605576.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            16326..17195
                     /gene="pdxY"
                     /locus_tag="OO566_002312"
     CDS             16326..17195
                     /gene="pdxY"
                     /locus_tag="OO566_002312"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6605577.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQV
                     KAANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAV
                     NNVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGM
                     RQPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDR
                     IAKPEHYFSATKL"
     gene            complement(17239..17844)
                     /gene="gstA"
                     /locus_tag="OO566_002313"
     CDS             complement(17239..17844)
                     /gene="gstA"
                     /locus_tag="OO566_002313"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6605578.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(17950..19452)
                     /gene="dtpA"
                     /locus_tag="OO566_002314"
     CDS             complement(17950..19452)
                     /gene="dtpA"
                     /locus_tag="OO566_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6605579.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(20063..20698)
                     /gene="nth"
                     /locus_tag="OO566_002315"
     CDS             complement(20063..20698)
                     /gene="nth"
                     /locus_tag="OO566_002315"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6605580.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(20698..21393)
                     /gene="rsxE"
                     /locus_tag="OO566_002316"
     CDS             complement(20698..21393)
                     /gene="rsxE"
                     /locus_tag="OO566_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6605581.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(21397..22017)
                     /gene="rsxG"
                     /locus_tag="OO566_002317"
     CDS             complement(21397..22017)
                     /gene="rsxG"
                     /locus_tag="OO566_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6605582.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(22021..23079)
                     /gene="rsxD"
                     /locus_tag="OO566_002318"
     CDS             complement(22021..23079)
                     /gene="rsxD"
                     /locus_tag="OO566_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6605583.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         5716 a         5982 c         6033 g         5423 t
ORIGIN      
        1 catgtcaaag tcagcagcgg gtacggtcag ccagccgggg cgcaatgtcc gggcaaaatc
       61 aggtttaaac cgttcgatac tggatcaggg ctggtatgaa atgcgccgcc agcttgagta
      121 caagcagctc tggagtggcg gtcaggtgct tgctgttccg ccagcgtaca caagccagcg
      181 ttgcgcgtgc tgtggtcata cagcgaaaga aaaccgcctg tcacaaagta aattcagatg
      241 ccaggtatgt ggatatacag cgaacgccga tgtaaatggc gctcgtaaca ttttagcggc
      301 ggggcacgcc gttcttgcct gtggagagat ggtgcagtca ggccgctcgt tgaagcagga
      361 acccaccgaa atgattcagg cgacagcttg aacgtagcag ggaaccacgt ctttcagggc
      421 gtggaggatg tcaactatcc ccaatccagc ccttgtggcg aacttgaaaa tcccgcctca
      481 cgcagcagcg taaacatcgg cgtttgccgg accggaagat cattcacttc tgttagcgta
      541 aagcgcagcc ttggttcgcg acgcagtgcg gtggtcagcg cctggaaaat ctctggggcg
      601 agtaattcct ccttttcctg ccagaccagc atttttttgc cgccttgcgg cagatagagt
      661 aacaatttgc cgccagaaac gaccaccagc gcacccgccc gacgcgttgg taccagcgct
      721 gccggatgtg caggccaggg cagaagattt ccccacacat tagccggatc gttggcggag
      781 agcgccactg gtgtatagtg gcgtgtttgc gtggcttgtg tcgccagatc gcgcaatcgg
      841 tcaatagtca gacgttcagc gaattgcgcg ccacccagac cttctacaaa acgacctcgc
      901 ataattcgcc cggagtcttc catacttcga caaagtgttt gcatcgatgg aaacccgcca
      961 gggatatttt cggctatcac cgcctgacga ctgatgatgc cgtagcggtc gagcatattt
     1021 tccgccagcg ccagcatcct ttcggtatcg tttagtggct ccacctgcaa taacgaccag
     1081 cgtccagcca gatttggtgt gttgtaagat acccgcggcg agacgggttg tgcatagaca
     1141 ggacgcccac gacgagcccg gtgactgcgg cgagttgagg tgcgtgcgtt ggaactgctg
     1201 cgggtgaggg cgcgtaacgg tgcccaaata tcgctggtaa tgacgccttg ccagaccagc
     1261 gcccataacg cctcttgcag tgccgaggaa tcaaccgatt cgccgatttt atcgcgtatc
     1321 cgttggctga tttgttgtgc aaaccaggct cctccgtcag ccagaacagc gattatcgct
     1381 tgctgcagcg ccgaacgatt cgcctgatcc gcttcggcgg gagtgaacga ttctgcggca
     1441 tatttctgta gatgcagtgc caccaggccg tcatcttcac ccagcttttt tttgtcccga
     1501 ccagataacc gctccggttg ccagtaattc atcgagcatt tctggcgaat agtcgcgtac
     1561 gcgggcaggc aggatctggc tttcccagag tgaggcgggt aaaccgactc cggcaagctg
     1621 ttcgatcacc cgcatcacgc catctacgcc ctcataaacg cctggcgatg ttgaggcaaa
     1681 gagcgccggg ctaccatcgg tggcgggtaa tacgccctga cgttccagca gcaatcgcgc
     1741 ataggtcgtg gctgcaacgg gacgcgtcgc ttctctggcg gcttgcagcg agcgcaaacg
     1801 cagacgacga aatacttcat cgctcaccca gatgtcttgt tgcagattca tcaccagacc
     1861 ctgttcacgc agttgctgaa gctgctcttc gacaatggca ataccgagac taaactcatg
     1921 agccagttgt tcagccgtga ccagagcatg agcccggaga tagcgcagaa agaggtcgcg
     1981 aagcgggtaa ctgactctat gtaaataaat ctctggcaat gactctggta gtcgtacgcc
     2041 gagggcatca cgcagcctgg cggcatcatc catacaggca agacgctcct gtccgctaat
     2101 catcgcaggg aagattcgtt ttactgcaag aagattttcc agatacgacg caacctcttc
     2161 actgctgcct gtatgccgtt gcgccaggtc ttcaacggtc attggcccca gttcgcgcag
     2221 caggtcgaac aatccttctt cacctttcgc tcttctgcca ggagccagtc gttgcaactc
     2281 ttcttccacc tggcgaatga cctgcgggtc gagtaattcc cccggatcga cctgtccgag
     2341 tagattgcgc agtaactcgc tgtccagcga cagtacggat gcccggcgct ctgccagcgg
     2401 ggcgtcgctc tggtacataa attccgcgac atagccgaac aataaacttg tggcgaaagg
     2461 cgagggcgta gtggtcgtta tatcggatat ttgaatttcg ccaccgttca ggcgacgcat
     2521 caaacgttcc agtgcgggaa gatcataaac atcttgcaga cattcgcgta gggtttcgag
     2581 aatgaccgga aaatccggat atccctgagc gatttccagc aactgactgg cgcgcagccg
     2641 ttgttgccaa agcggggtgc gatggcccgg cgtgcggccc ggcattaata atgcccgcgc
     2701 ggcgcattcg cgaaaacggg cggcgaaaag tgccgagctg cctaccgcct cgcggacaat
     2761 ttgcagcaac ttttctggtt caaacaaaaa aatcgcggcg tcgggcaatt taccatcagt
     2821 gtcaggaatg cgcgcaacaa tgccgtcatc actggcgacc acagacgcgt cagcgcccca
     2881 tagggcatgt attcgcccgg caatcgccag cgcccagggt tcatgcaccc gtcttccata
     2941 gggagagtgc aaaataatac gccagtcgcc aatttcatca cggcaccgtt cgaggaccag
     3001 atggcgactc cccggaacga tgcccgtcgc gttgcgttgc tcgtcaatca acccctgaat
     3061 attggcgttc gtattttctt ctgccagcca cgggggaata gtcccggaaa agaacgcgcc
     3121 atccgccagt aaatgaagaa aatcgccgat catctcgcct aattcagccg gacgtccgtt
     3181 accttcacca cgccagaagg ggagccgggc agaacgaccc ggagcaggag tcacaatcac
     3241 ctgatcgcgg gtgatttgct ggatccgcca tgaggtagcg ccgagagtga taatgtcgtt
     3301 cacccgcgac tcatatacca tctcctcatc cagttcaccc acccgccgcg aaccggcctt
     3361 ttcttcacct tcgggtaata acacgctata catgccacga tccggaatgg tgccgccgct
     3421 ggtaaccgcc aacaattgag cgccaggtcg ggcggtcaat atcccggtct ccctgttcca
     3481 gaccagtttg gggcgaaaag cagaaaaatc gccagaggga tagcgcccgg aaagcatatc
     3541 cagcgtggcg tcaaaaacac gtcttggcag atctttccac ggtgcggcac ggcgtacgcg
     3601 ggagtaccat tcgtctacct gtaatgcatc catcgccgcg gcggcaacgg tttgctgcgc
     3661 aaggacgtcg agaggattat gcggtggtgt caggttttcc agcctgcctg cgaacataca
     3721 ctctacaatg actgcggaat cgactaaatc acgccgggta cgggggaaaa acagcccttt
     3781 agatacaccg ccaacctgat gtccggcgcg accaatgcgt tgtaacccac tggcaacaga
     3841 aagcggcgtt gccacctgaa tcaccagatc caccgcgccc atatcaatcc ccagttcaag
     3901 actggaggtt gcgaccacgc agcgtaattc acccgatttc agcgcctgtt cggtgattgc
     3961 tcgttgttct ttagagacgg agccgtggtg tgagcgggca ataaaaacgt cgctactttg
     4021 tacacggtta gaggttgcgc cggaggtcga ctcgaaatgg gccgcatcaa cggcgataga
     4081 cggggaacgc tgtaagcgtg cggcgtaaag ctcatttaat cgtgccgtca gtttttccgc
     4141 cagcccgcgt gaattagtaa agacaatggt cgagcgatgg cgcaacactt catcaaggat
     4201 acccgtttca atatatggcc agatggagcc ttcccggccg gcatggctgt cttcgccggt
     4261 gccgctggcg accgatgaga catcatccat attggcgacc ggtacgacaa ttcgtatctg
     4321 cggatggcgc attgcgggcg ggttgactac cgtaaccggg cgatcgccac caagaaatgc
     4381 tgccacatcg ctggctgagc gcacagtggc agaaaggcca attcgctgtg ctgaggtgtg
     4441 gagcagcgca tcgagccgct ccagacttaa cgccagatgc gcaccacgtt tactgcccgc
     4501 taccgcgtgg acttcatcaa taattaccgt ttcgacgccg tgtagcgttt cgcgcgcgcg
     4561 ggaggtcagc atcagataga gtgattcggg tgtggtgatc agaatatccg gcggattacg
     4621 ggtgagtttg ctgcgttcct gtgcaggcgt atcgccagta cggatcccta cgcgaagatt
     4681 gacttccgtt tcgccgcgcc gccgccgttc atcggcaata cccttcaaca ggatctgcaa
     4741 gttgcgctga acgtcggtgc ccagggcttt tatcggtgaa atatagagga tgcgtgaggt
     4801 tttacgctta tgcgcctcgc gggtatcttc gccgccttcg cggaagagcc gatcgagggc
     4861 gtagagaaat gctgccagcg ttttcccgga gccagtcggt gcaatcacca gcgcatgttc
     4921 gcctcgcgcc gccacatgcc aggtttgcga ctggacagcg gtcggctgtt taaaggcgcg
     4981 aagaaaccag tcgcgggtcg ccggtgaaaa cacgtccggc aggagcgatg aagggtctgg
     5041 attatctgcc attaacgcta ttccccgtga atatcatgct tgatgcgtgg aatagcctca
     5101 ctatagatgc ctgaatcatt gcatgtcatc aggcatcgac tcgattacac ctcttcggcg
     5161 gcaggtagcg gccagcctcc cagacgtttc cagcggttga cgatttcaca aaacagcaca
     5221 gcagtgcgtt cggtgtcgta cagcgcggag tgcgcctggg tgctgtcgaa gtccatgcca
     5281 gcggtctggc aagcctttga caatacggtt tgtccgagtg ccagcccggc cagtgcagca
     5341 gtgtcaaaag tggcgaaagg gtggaacggg ttacgtttca gcgaggcgcg ttctgcggcg
     5401 gccatcataa agctgtgatc aaaattggcg ttgtgcgcca ccataatggc gcggttacag
     5461 ccgctcgctt taataccttt acgtacaact ttaaaaattt cgtgcagcgc ctcgtattcg
     5521 ctgaccgcgc cgcgatcggg atcgttcggg tcaatgccgt tgaaggcgag ggcttctggt
     5581 tgcaaatttg cgccgacaaa tggttcgacg tggaaatgta atgtggtgtc cggcatcagc
     5641 cagccttgtt catccatttt cagggtgatg gcggcaatct caagcagcgc atcggttttg
     5701 gcgttaaatc cggctgtttc aacatcgatc acaacaggat aaaaaccacg aaaacggtcg
     5761 cacagaccgg taagttgagc gttatcggac atctgattct cttaatacgg ataaattgca
     5821 gcgcgcatta tgacaaatat tacttgtcga tgcagtaaag atgcgggcgc gatgagttca
     5881 cgcccggcag gagattagtt gcccagaccg cgaccggcgt ctttctcttc gattaactca
     5941 attttgtaac cgtccggatc ttccacaaac gcgataaccg tagtaccgcc ttttaccgga
     6001 cccgcctcac gggtcacgtt acccccgttt tgacggattt tttcgcacgc ttcagcggcg
     6061 ttatctacgc taagcgcgat gtgaccataa gcagtgccga gttcgtattt atccacgccc
     6121 cagttgtagg tcagttcaat caccgcttct tcggtttccg ggccgtagcc aacaaacgcc
     6181 agtgagtatt tgtattccgg gttttcgctg gtacgcagca gtttcatgcc cagcactttg
     6241 gtataaaaat cgatggagcg ttgcaaatcg ccaacgcgca gcatggtatg aagaagacgc
     6301 atctttttat cctcaaaatg gctaacagat tcaaaatgtt gttttagtat agcggcggct
     6361 ttacgccgct cgcaatgttg gattacaacg tcgggtaatc ggtatagcct tccgcgccgc
     6421 caccgtagaa actttcggca cgctgtgggt taagctcagc tttgagctgc aagcgggcga
     6481 ccagatccgg gttcgcaatc cagtcacgac caaatgccac cgcatcaatt aaccctttgc
     6541 cgatcagcgt ttcagctttt tctactgtgt atgcacctgc gccgataatc ggaccgtgga
     6601 aacgggcgcg tactttttcg cggaacgcat cagtatacgg ttcacccccc gcccaatctg
     6661 gttctgacat atgcagataa gcaatgccgc gtttacccag ttgttcaatc agatacagtg
     6721 catcggcttc ttcattcggg ccgttatctg tgttctggaa agtaccgatt ggcgaaacgc
     6781 gaatgccaat gcgatcggca ccccattctt caatcccggc atcgaccact tccagtacca
     6841 aacgtgcgcg attttccacg ctgccgccgt actgatcggt acgatggttt gaagaagggg
     6901 aaaggaactg atgcagcaaa taaccgtgag cagagtggag ctctaccaga tcaaaaccgg
     6961 cttcacgcgc gttagcaatg gcctgacgga aatcattgac gatacccgga atctcttcca
     7021 gttcaagcgc acgcggcatg gatgtttcaa cgcggatcgc ccgaccattt tcatcgcgca
     7081 gagaagtacg tgttcccgcg ctaagtgctg aaggcgctac cggtgcctga ccgccaggtt
     7141 gcaggctggc gtgagaaatg cgtccggtgt gccacagctg caaggccata tgaccatttt
     7201 cagcatgaac gccagcggtg atttttttcc atgcggcaat ttgctccgga ctatggatgc
     7261 caggcgcacc tgcatatcct tttgcctggg cagaaatttg cgtggcttca ctaataatca
     7321 aaccggcact ggcacgttgg cgatagtatt ccgccatcaa cggggtagga atgtcacccg
     7381 gttcaatact gcgcagacgc gtcagcggtg ccataaaaat acggtttgcc gccgtgatcg
     7441 cgcccacttt cagtggggaa tacagttttt cagatgacat aaaggctccc ggtcgtctgg
     7501 tgataaaaaa tgcctgctaa acggcaggcg tcgcaataat gttttttaca tgggccagcg
     7561 cgttttccag tggctcgaaa ctgcgcgaaa ttttggcctg cagattcgcg ccaagccata
     7621 gtgcgtaaag cacttgtgcc tgttgtagcg gttcgccaca aaaggttaaa caatggttct
     7681 cacggccatt ttccagagcc tgcgagagca gggcgatcac gccgcgagca cctttatcca
     7741 tcgcgctgcg catatcttct gacagatcgc acacttcggc agagagtttt actgtcaggc
     7801 aaccactgat ggttccatgt tggcaaaact ggttcagtgt ttgctggtaa taagccagta
     7861 tgcggtcgcg gtagttacct tcgccggatt gcagcaactc agccagtcgc tggtgatatg
     7921 cggcgtaatg acgctcaagc atggcaacgc caaacgcttc tttagagcga aagtagtgat
     7981 agaaggaccc tttcggcact tcagcggttt ttagtaattc gcttagcccc atcccggtga
     8041 atccacgttg caggcaaagt tgctcgcccg tcgccaagag atgttcgcga gtatcatgtt
     8101 cggtgtgttt gttcatgcgt tggagtgtag tagaccagtc ggtctagtgc aagcaaagtt
     8161 gctactttgg acattaacag cttcaataat ggtaataatc ttatctgagg agggcgaggt
     8221 ggcggagcaa ttagagttct ttcccgtcca gagcccgtgc cggggaattt gccagtctga
     8281 cgaacgcggg ttttgtcgcg gctgttttcg tagtcgggat gagcgtttta actggaataa
     8341 aatgagcgat ggcgaaaaac aagaggtgct gcgcctttgt cggcagcgat taatgcgcaa
     8401 attacgcgca aataagccag cgtcatcaga cgaaccggag caaccatcac tcttttgaac
     8461 ctaatcttgc gtatactcct ctcatatctg ataagaggaa gcgattatgg ttcagcgtat
     8521 tactattgcg ccggaaggcc cggagttttc ccgttttgtg atgggctact ggcgattgat
     8581 ggactggaat atgtccgccc gccagctggt cagttttatt gaagagcatc tggatctcgg
     8641 cgtgaccacc gtggaccatg ctgatattta tggtggctat cagtgcgaag cggcgtttgg
     8701 cgaggcactg aaactggcac ctcacctgcg tgaacggatg gaaatcgtca gtaaatgcgg
     8761 tatcgcgacg accgcgcgtg aagaaaacgt cattggtcat tacatcactg accgcaatca
     8821 catcattaag agcgccgaac agtcgctaat taatctcgcg accgatcatc tggatctgct
     8881 gttaatccac cgaccagacc cgttaatgga tgccgatgaa gtggcggacg cgttcaaaca
     8941 tctgcatcag agcggcaaag tgcgtcattt tggcgtatcg aactttacgc ctgcgcaatt
     9001 tgccctgttg caatcacgtc tgccgtttac ccttgccact aatcaggtgg aaatatcccc
     9061 ggtgcatcag ccgttactgc tggatggcac gctcgaccaa ctacaacaac tgcgtgttcg
     9121 tccgatggcg tggtcctgcc ttggtggtgg tcgtctgttt aatgatgatt atttccagcc
     9181 gctgcgtgat gaactggctg tggtggcgga ggagttaaac gcgggctcga ttgaacaggt
     9241 ggtttacgcc tgggtattac gtttaccgtc gcagccgctg ccaattatcg gctcaggtaa
     9301 aattgagcgc gtacgggcag ctgtcgaagc agaaacactg aaaatgaccc gtcaacaatg
     9361 gtttcgtatc cgtaaagctg cactggggta cgacgtaccg taagcacttt taggaatagc
     9421 cgccgttcaa aaatgtgtca ctggtttaca cttattcagg aatgcacaat gaacggaggt
     9481 cctatgaaac gttttagtct ggccattctg gcgctggttg ttgcgaccgg cgcacaagct
     9541 gccagtgaaa aagtcgagat gaacctcgtc acgtcgcaag gggttgggca gtcaattggt
     9601 agcgtcacca ttactgaaac tgataaaggt ctggagtttt cacccgatct gaaagcatta
     9661 ccccccggtg aacatggctt ccatattcat gccaaaggaa gctgccagcc agccaccaaa
     9721 gatggcaaag ccagcgccgc ggaatccgca ggcgggcatc ttgatccaca aaataccggt
     9781 aaaattaacc atcggaaagg cgatgatggc agccgtgtag cccgccagtt gaaatgcata
     9841 cgcgacgtta ttcgtgaagt gcgcacaggc ctaggtacaa aagccaagcc acgccgacat
     9901 gctcaataga aaaaaccacg gctcattgag cgtatgtccg gcaagaagca gtgccgcaat
     9961 ggctcccagt aaactgccag cgatgcgtcc gaggcttttg ctgatgacac cgccaacggt
    10021 gggaaagcta accactgcag ccgaggtcat cgcccaatag ggttcatcca gattcaaata
    10081 ataggcaacc gtcagcgcca gacacatggc aatggtattg cgtaacgcat aacgccattg
    10141 cgccagcgtg gccctgaacc agcgcaaagc ggacgggaac gcgttgggcg agacgaaccc
    10201 aggggacgtt aggtttcaca tccggaataa ggcttgagtc actttcaacg ctctgatcat
    10261 aaatcgcccg accgatactg gaaacgtgac cctgtaacgt tttgttatca ctatagaggg
    10321 taatttgtgc tggtgcacct tcgcggatat ggcgcaactt ggtttcttca aaataaccaa
    10381 tgacgtaaaa cgagtggctg tcgacaaggg caaacagcgg tttcccggtg tcagcgtaat
    10441 cgccgatgcg ggtagtgagg ttagtcaccc atccactcac cggggcgcga atttccgttt
    10501 gcgccagttg ccattgtgtc tgtttcagcg tcgcctgtgc ggcattaaca ctggcctgca
    10561 tcgctttaac attgaggtta gcggtatcga gttcttcggc aaaaataaaa ttttgtgaga
    10621 gatgacgacg acgattcgct tcgttattgg cttttgcgag gtcagattgc gctttcgcca
    10681 gctgagcctg tgcgttcagc tcggcgatct gaaagggcgt tttatcgatc gtgagcagaa
    10741 gttcccccgc gttaaccagt tggttgtctt ttatattcag ctcaacaata cgtcccgaca
    10801 cctgtggggt gatagaaacc tgttctgcgt gtattttccc gtcgcgtgtc cacggagatt
    10861 gcatgtaata attccacagc caccatccgg caagaaccgc aactatcgct acaatgattg
    10921 ttgagaaata cttaattgtt ttcattgaca tagttaccac gcaatcagta ttgccagagc
    10981 aaggcaaacg caaatcgcaa acagcgataa atccattaac aagggatgcc agatgtcacc
    11041 ggcgtagatc cagccacgaa gcagacggtg tacgacaagc cagatgacga atccaaacgc
    11101 gaatgctttg aaaaacggag gaaaatagac ggacgcaccg aacaccagat cttgtaaggg
    11161 caatcctgtt gcattgagca taaacttcac aaagtagatt cctttacgac cgaatatatt
    11221 gcgttagatt aataaatatt ctttaagtgc gaaaaattta cgcgcaattt ctgaaaaata
    11281 tgtctgacgg taaccaaatg cagcaataca tttgttttag caatacaatt gctgcacact
    11341 attctaaaag ccgcataata tcttagcaag ctaattataa ggagatgaaa ttggaatcgc
    11401 cactaggttc tgatctggca cggttggtgc gcatatggcg tgctctgata gaccatcgcc
    11461 tgaaaccgct ggagttaaca caaacccatt gggttacgtt acacaatatc catcagttac
    11521 ctccagacca gtcgcaaatt caactggcaa aagcgattgg catcgagcag ccatcactgg
    11581 tccgtactct ggaccaactg gaagaaaaag ggttaatttc gcgtcaaact tgtgccagcg
    11641 atcgtcgggc taaacgtatt aaactgacgg aaaaggcaga gccactgatc agcgaaatgg
    11701 aagctgttat taacaaaacc cgcgcggaaa tattacatgg catctccgca gaggaactgg
    11761 agcaactgat tacgctcatc gcaaaacttg agcataatat cattgagtta caggccaaag
    11821 ggtgaaatga agggggcgtg tggtcagtaa cctgaccaca cgcaaactta tttagcgcgg
    11881 agaaacggtc acctgactgc cattgctggc cagtacgaca cgttggcccg gagagaaacg
    11941 agtgttgcct tgtttctgta ccaccatgat ggtattacca tcgtctttac gaatttccag
    12001 ctcgacaccc tgcgttttgt tcattgcact ctgtacgccc tgaccagcta cgccacctgc
    12061 aacagcgcct gctgcagtag ccagagaacg cccggttccg ccaccaacag tattccccag
    12121 gaaaccacca agaacagcac cgccaattgc accgataacg ttggaatcat caccgccctg
    12181 aatctgtacc ggacgtacgt taacgatggt gccatagctg acattctgta cttgtttcgc
    12241 ttcagaagcg gtataaacat cccctgacag ggtgtcgtta ttaacacaac cgacaagaga
    12301 cagacctacc attgaaacaa ccaatacgcg tttaatcact gaaaaatctc ctgttcacca
    12361 tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg gcatatgtga atccatggtt
    12421 acatgttcta ccaaatatga gcaaatcata atgaaagtag gatcaaccaa ttataaacaa
    12481 aacattcatg atgattattg atttgaagat aatgttttac taccaacggt aaaaatcggt
    12541 cattccgcat ggtattcacg aaaagtttat gtttgagtgg tgcgcaaacg tcagattgaa
    12601 aaaggatgaa ctatgaaatc gggccgcttt attggcgtta tgtcaggcac cagccttgat
    12661 ggtgttgatg ttgtgttggc gacaattgat gaacaccggg tcgcacagct ggcaagcttg
    12721 agctggccga tcccggtgtc tctgaaacag gctgtactgg atatttgcca gggccagcag
    12781 cttacacttt cgcagtttgg acagcttgat actcaactcg ggcgactttt tgctgatgcg
    12841 gtcaatgcct tgcttaagga acaaaacctg caggcgagag atatagttgc gatcggctgt
    12901 cacggtcaaa ccgtctggca tgaaccgacg ggcgtggcac cacacacttt acagattggc
    12961 gataacaatc aaattgtggc acgcaccgga attacggttg tcggtgattt tcgccgtcgc
    13021 gatattgcct tgggaggaca aggcgcaccg ctggtacctg cgttccatca tgccttgctg
    13081 gctcacccaa ccgagcgacg aatggtgctc aatattggcg gcatcgccaa tctgtcactg
    13141 ctcattcctg ggcagccggt tggcggctac gataccggtc ctggtaacat gctgatggat
    13201 gcctggatct ggcgtcaggc aggtaaacct tacgataaag atgccgagtg ggcacgggcg
    13261 ggtaaagtta ttctcccact gctgcaaaat atgctcagcg acccgtattt ctcgcaacct
    13321 gcaccgaaaa gcaccggacg cgaatacttt aactacggct ggctggagcg ccatttacgc
    13381 cattttccgg gtgttgatcc ccgtgatgtt caggccacac tggcagaact caccgccgtg
    13441 accatttctg aacaagtttt gttgagcggt ggctgcgaac gattgatggt atgtggtgga
    13501 ggtagtcgta atccgctact catggcgcgt ctggcggtat tactgctagg cacagaagtc
    13561 accaccaccg atgccgttgg cattagtggc gatgacatgg aagcattggc tttcgcctgg
    13621 cttgcctggc ggacgctggc gggattacca ggaaatctgc cttccgtcac tggcgcaagc
    13681 caggagacgg tactgggggc tattttcccc gccaactcgt gatatcagaa ttaactgaat
    13741 tttttcttat cgtgtctgcc gttacacttg gaagattaag gagggtattt gccctccgga
    13801 ccaggaaagt cttcgggata tgactatgaa aaaactgtta attatcattt tgccggtcct
    13861 tctctccggt tgtagcgcct ttaatcaact ggttgagcga atgcaaaccg atacgctgga
    13921 ataccagtgt gatgaaaaac cgttgacggt caaactgaat aatccgcgcc aggaagtcag
    13981 ttttgtttac gataatcaac tactgcatct caaacagggc ctttcagcct ctggcgcgcg
    14041 ttactccgac gggatctatg ttttctggtc gaaaggtgag gaagcgactg tctataaacg
    14101 cgaccgcatc gtcttgaata actgtcagtt acaaaatcca cagcgttgag atttttccag
    14161 gggcggcgca caatagcgtc acccactgac aatccgtaaa gaaaaccatg tctgataacg
    14221 acgaattgca gcaaatcgcg catctgcgcc gtgaatacac caaaggcggg ttacgccgcc
    14281 gcgatcttcc cgccgatcca ttaacccttt ttgaacgttg gctctctcag gcttgtgaag
    14341 ccaaactggc ggaccctacc gcgatggtgg tcgctaccgt ggatgaacat ggtcagcctt
    14401 atcagcgcat cgttttactc aaacattacg acgaaaaagg catggtgttt tacaccaacc
    14461 tcggtagccg taaagcacat caaattgaaa ataatccgcg cgttagcctg ctgttcccgt
    14521 ggcataccct tgaacgccag gttatggtga tcggtaaagc agaacgactt tcgactctcg
    14581 aagtgatgaa atattttcat agccgcccgc gtgatagcca gattggtgca tgggtttcga
    14641 agcagtccag tcgcatttct gcccgcggta tccttgaaag taaattcctg gagctgaagc
    14701 agaagtttca acagggcgaa gtgccattgc cgagcttttg gggcggtttt cgcgtcagcc
    14761 ttgaacagat tgagttctgg cagggcggtg agcatcgcct gcatgaccgc tttttgtacc
    14821 agcgtgaaaa tgatgcgtgg aagattgatc gtcttgcacc ctgaaaagat gcaaaaatct
    14881 tgctttaatc gctggtactc ctgattctgg cactttattc tatgtctctt tcgcatctgg
    14941 cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac atggagattt tgatggcaag
    15001 cagtaacttg attaaacaat tgcaagagcg ggggctggta gcccaggtga cggacgagga
    15061 agcgttagca gagcgactgg cgcaaggccc gatcgcgctc tattgcggct tcgatcctac
    15121 cgctgacagc ttgcatttgg ggcatcttgt tccattgtta tgcctgaaac gcttccagca
    15181 ggcgggccac aagccggttg cgctggtagg cggcgcgacg ggtctgattg gcgacccgag
    15241 cttcaaagct gccgagcgta agctgaacac cgaagaaact gttcaggagt gggtggacaa
    15301 aatccgtaag caggttgccc cgttcctcga tttcgactgt ggagaaaact ctgctatcgc
    15361 ggcgaacaac tatgactggt tcggcaatat gaatgtgctg accttcctgc gcgatattgg
    15421 caaacacttc tccgttaacc agatgatcaa caaagaagcg gttaagcagc gtctcaaccg
    15481 tgaagatcag gggatttcgt tcactgagtt ttcctacaac ctgttgcagg gttatgactt
    15541 cgcctgtctg aacaaacagt acgatgtggt gctgcaaatt ggtggttctg accagtgggg
    15601 taacatcact tctggtatcg acctgacccg tcgtctgcat cagaatcagg tgtttggcct
    15661 gaccgttccg ctgatcacca aagcagacgg caccaaattt ggtaaaactg aaggcggcgc
    15721 agtctggttg gatccgaaga aaaccagccc gtacaaattc taccagttct ggatcaacac
    15781 tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc tttatgagca ttgaagagat
    15841 caacgcccta gaagaagaag ataaaaacag cggtaaagca ccgcgcgccc agtatgtact
    15901 ggcggagcag gtgactcgtc tggttcacgg tgaagaaggt ttacaggcgg caaaacgtat
    15961 taccgaatgc ctgttcagcg gttctttgag tgcgctgagt gaagcggact tcgaacagct
    16021 ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc gcagacctga tgcaggcgct
    16081 ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt aaaactatcg cctccaatgc
    16141 catcaccatt aacggtgaaa aacagtccga tcctgaatac ttctttaaag aagaagatcg
    16201 tctgtttggt cgttttacct tactgcgtcg cggcaaaaag aattactgtc tgatttgctg
    16261 gaaataatgc attaagtgga aagggggagt gagaaatcac tccccctggt ttttatacag
    16321 ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt tgtttatggt catgcgggta
    16381 acagtgcggc agagtttccg atgcgccgcc tgggcgcgaa cgtctggccg ctgaacaccg
    16441 ttcaattttc taatcacacc caatacggca aatggactgg ctgcgtgatg ccgcccagcc
    16501 atttaaccga aattgtgcaa ggcattgcca ttgccgccat tgataaatta cacacctgtg
    16561 atgccgtatt aagtggctat ctgggatcgg cggagcaggg tgaacatatc ctcggtatcg
    16621 tccgtcaggt gaaagcagcg aatccgcagg cgaaatattt ttgcgatccg gtaatgggtc
    16681 atccggaaaa aggctgtatc gttgcaccgg gtgtcgcaga gtttcatgta cggcacggtt
    16741 tgcctgccag cgatatcatt gcgccgaatc tggttgagct ggaaatactc tgtgagcatg
    16801 cggtaaataa cgtcgaagaa gcggttctgg cagcgcgcga actcattgag caagggccac
    16861 aaattgtgtt ggttaaacac ctggcgcgag ctggctacag ccgtgaccgt tttgaaatgc
    16921 tgctggtcac cgccgatgaa gcctggcata tcagccgtcc actggtggat tttggtatgc
    16981 gccagccggt aggtgttggt gatgtgacga gcggtttact gctggtgaaa ctgcttcagg
    17041 gggcaacgct gcaggaggcc ctggaacatg tgaccgctgc agtctacgaa atcatggtga
    17101 ccaccaaagc aatgcaggaa tatgagctgc aagtggtggc tgctcaggat cgtattgcca
    17161 aaccagaaca ttacttcagc gcaacaaagc tctgaaatat ctcatttcgg cccacaacga
    17221 cctgtgggcc gatggctatt actttaagcc ttccgctgac agcgcgtctt gtacttccgg
    17281 acgttcagcc atacgttgca taaatgccgc aatgtgctct aacccttcca gattcagttt
    17341 caccgcgtat gcccagcgca gaaccgtaaa cagataggca tcagcaattg taaatctttg
    17401 cccgcagatc cagtgctcat ccttcagtgc ctcgttcaca tattgcagct tcttatccag
    17461 ctgcgcgcga actgtcggtt tgtactcttc cggtgtatct gggcgaaaca gaggtgtgaa
    17521 acctttatgc agctcggtgg cgatgtaatt cagccattcg atggttttat agcgggaaat
    17581 actgtttacc ggtgccagca actggcggtc ggggacgctg tcggcaagat actgcataat
    17641 cgcaacgcct tccgtcagca aagtaccatc gtccagcagc aatgcaggca cctgcccctt
    17701 agggttaacg gaaaagtaat cgtcaccgtt ttcgagacgt tttttcatta aatccacact
    17761 gacgagcgta aaatcctttc cgctctcacg cagggtaata tgggaagcga gagagcaggc
    17821 gcccggtttg tagaacaatt tcatcggtaa ctccttttgt ctgtggttta aagtatggta
    17881 gtgcgttgct gaacaaaaaa aaagccgcta acgaaaagtt agcggcttgt aaaagagttt
    17941 ccctgaattt tacgctacag tagctttcgc cgctttgtct gcagcgtcat cctgcgtcat
    18001 gcggtgcagt ttaggcgcgg tcagcagcat cagtactgca ataacggcag tagcgacacc
    18061 aatctgcaag aatacgcgac catagacttc cagtgacatc agcgggtcgg taacgttatc
    18121 cggcacagcc atcatacccg caacataacc accaatcagg tttgcaccgg cagtggtcag
    18181 gaaccagcta cccataatga agcccatcag acgctgcgga acgagttgtg caaccattgc
    18241 cagacccaga ccagagatca tcagttcccc gatgctctgc aggccatagc ttgcgaccag
    18301 ccagctgaca gacacgatac ctgcgtcaga cgcgaatttc gcacccagcg gcagaatcag
    18361 gaacgcacca gaacacatca ccatgccgat tgcaaacttg gttggcatcg gcagggtatc
    18421 acccatcttg ttatagatag cggccagaat cggactaccg atgatgatcc agaacgggtt
    18481 cagtgcctga tactgctcag gttctacggc cagacccaga atggagtgct caacgttacg
    18541 aatcgcaaag aagttcagtg acgttggcat ctggctgtac agcacgaaga agatgattgc
    18601 ttcgagcatc aggatgaagg caacgatcat tttacgacgc gcagcacctt tcatcgcgaa
    18661 agcttcttta ccgaagataa ccacaatacc gaaggcaaca acgcccagcg ccatacgcgc
    18721 aacttcctga ttgtgcagta gccaggtggc aacagcgatc agcgccacaa caccaataat
    18781 ggtcagcaac aggttacggt agttgactgg ttcaaagtct ggttttgaac cgtactgttt
    18841 aacccagcgt tggcagaagg cgaagttaac gatagtgatc agcaggccta caacgctcaa
    18901 cgcaaacgca acactccagc cgtatttcgc ggccagccac ggggtggcta tcatagagaa
    18961 gaaagagccg atgttgacgg acatgtagta catggtgaat gcaccgtcca gacgtgggtc
    19021 gtttttctca tagcatgtag aaagcagaga agacgggtta gctttaaaca ggccgttacc
    19081 gaccgcaata gccgccatac ccatataaac aataccggcg tcgtgaccag accaggcaac
    19141 cagagcataa ccaatcgcca gcacaatagc gccgagcata attacgcgtt tagtacccag
    19201 taccttgtca cctaaccagc cgccgatagc gaccagacca taaaccaggg cactaaagga
    19261 agagaaaagg gtgattgaat ccgcttcaga catacccagt tgtttaacca ggtaaacagc
    19321 cataattcct tgtaggccgt aataaccaaa acgttcccat aactcaatcg agaagatgag
    19381 atagaacgcc ttcggttgtt tgaaagcgtt caaactgacg ctttcagttg gtttttggtt
    19441 tgcagtggac acttttacct ctttttttac atcccatatt aacgggggtg ctttctcgcg
    19501 ttatgtctaa aaaacatacg cgcaattgtt atagtgggag gggaaacggc aggtaatgtt
    19561 cactatcttg cactttcaga caagtccttt gtaataatct gttacatata actgaggcgg
    19621 ggaattcaac cattccggga aatgttatcc agcgttaaat ttttcaaaaa acgtaacggc
    19681 agattttttc actgcattga gagggggatg atggctttac ggagatatgt tctgctattt
    19741 agcgtttttc ctagtgagat agtccagttt ctgaaaaata gccagtgtaa tgttttgcag
    19801 gtcaatattt gttgcgtgtg taaatttaaa tcattttaac ctgattcttg caatgttttt
    19861 acattgccac agcatcgtga tcttgatcac gaatgaatag aaaatttcgc tattaaaaaa
    19921 acaattaacc tgcatttttg gttatccgca aacggattac ctgatgataa cgctaagcaa
    19981 tggcatcatt gacgttaaaa agatgtttta aaagaggata aagaaaggtt atcaatgggg
    20041 taatcggtgt tacccctttt cttcagatgt caactttctc tttgtattca caaagatctt
    20101 caataataca agagccacag cggggcttgc gggcaatgca ggtataacgc ccgtgcagga
    20161 tcaaccaatg gtggcagtcg actttaaact ctgccggaac cactttcagt agcttttctt
    20221 ctacctgttc gacgtttttc cccggcgcaa attgagtacg attacaaacg cggaaaatgt
    20281 gcgtgtcgac agcaatagtc ggccagccga atgcagtgtt taatacgacg ttggctgttt
    20341 tacgacctac gccgggcagg gcttcaagcg cagcacgatc ttccggaacc tcgccattat
    20401 gttgttccag taatatacgg caggttttga tgatattttc tgctttgctg ttataaaggc
    20461 caatcgtttt gatataggtt ttcacccctt caacgcccag ttcaagcatc gctgcaggcg
    20521 tattcgccac cgggtagagt ttcgccgtcg ccttattaac actgatatcg gtcgcctgag
    20581 cggaaagcag tacggcaatc agcaattcaa aaggcgaact gaaattaagc tcggtggtgg
    20641 gatgaggatt gttctcacgc aggcgagtga ggatctccag acgttttgct ttgttcatca
    20701 gacattccct gtttcaccgt ttggcaatgc acgttcggca gctgcttcag cacggcgctt
    20761 tttcattctt tcatcaatca ggtattttcc tgccagcatc agtcccaagc caataaatgc
    20821 acctggtggc agcatcgcca gcaggaaagg ggagtcggtg tggaaaatct ccacgcgtaa
    20881 tacttttgcc cagctaccta acagcgcatc tgcaccgtca aacaacgtgc cattgccgat
    20941 aatttcgcgt agtgaaccca gcacgaacat ggcgcaggtt gcgcccgtac caattgaaaa
    21001 gccgtccagt gccgaaagcg ccggaccttt tttggcggcg aaggcttcag cgcggcccac
    21061 aacgatacag ttagtgacaa tcagcggaat aaaaatccct aatgattgat acaggccaaa
    21121 ggcgtaggcg ttgatcagca tctgtacagc gctgaccacc gaggcgatga tcatcacgta
    21181 aatgggaatg cggatctcgg ctggcgtcca gtgacgcagc gtcgaaatgg tcaggttggt
    21241 cagcgtcagt accagcgtag tcgcaagtcc taaacccaga gcgttagtgg cagtggacgt
    21301 gaccgccaat agcggacaaa ggccgagcag ttggaccagt gcagagttgt ttttccacaa
    21361 cccctgaaca ataacgtctt taatttcgct cacgctttat tctccacagg caggaagttg
    21421 agaaagttgt gccggtaacg tctgagcgta caatccggcg cgttttaccg cattaaccac
    21481 cgcgcgggga gtaatcgtcg cgccggtgaa ctggtcgaaa tcaccaccat ctttcttcac
    21541 cgcccagtgc gcatcatccg caccactgat tttttttccc gcaaaatggg tgagccagtc
    21601 agaaaggcgc aattcgattt tatcgccaag ccctggcgtt tcgtggtgct ctgtcacgcg
    21661 cgtgccaagt accgtgccat taaaatcggc tcccaccagc agctgaatcg caccggaata
    21721 gccatctggc gcggttgctt ccagaacggc ggctaccggt ttgtcatcct gtttggcgat
    21781 gtaaacccga tgctcacctt tacctaactc tggcgcagtt accagatagc aactctgtgc
    21841 gagcgcattg ttatagcgtt cggctggcag cacctgacca aataacgcct tttgttgcag
    21901 actggcctgt tcagcaattg tcgttttggt catctggttg atggccgcag ttaaccctgt
    21961 agaacccgct gcaaatagtg ccagtgtaat gccgtgtttt cggatagttt tcagcatggt
    22021 ttaccctttg cgatggccgt agacgcgcgg acgcgtgtag taatcgatca gaggaaccgt
    22081 gatgttcgcc agcaggacgg caaaagccac gccgtcagga tagccgccga aactgcggat
    22141 caaccagact aataagcccg ccagcgcgcc gaacatcagc cgaccacgat tggtcgtaga
    22201 agcagtaact gggtcagtca aaataaagaa tgcgccgagc atagtcgcac cagacagcag
    22261 atgaatttgc ggtgctgcca gtgtttctgg tgagaacaac cagcccaacg ttgcgcataa
    22321 cgccagcgtt actaagaagc tgaggggaat atgccagcga atcgctttct gccatagcaa
    22381 ccacacgcca ccagccagcc aggcgagatt tacccattgc caaccagcgc ccgccagaat
    22441 accgctgtag atcggatatt gcataatctg ttcaaccgaa tgacctgcgc ggacagaggt
    22501 tttgaatgta tccagcggtg tcgcctgact aatgccatca atacctaagc gtagcgtatt
    22561 catatcacca ccactggcgg tatgaccgct gaaaataacc tgtatggcgt cgataaaacc
    22621 agggatgttg accgcaattt catgtggcgg taaccagctg gtcatctgca ccgggaagga
    22681 gatcagtaag accacataac caatcattgc cggattaaac gggttttgcc ccagaccgcc
    22741 atacaactgt ttagcgatga tcaccgcaaa caccgtaccc agtacgacca tccaccatgg
    22801 cgctaggggg ggaatactta ccgccagcaa taagcctgtc agcaatgctg agttatcttt
    22861 caacgttgcg gctaccgact gcttgcgtag tttgagtacg agagcttcgg ctaacagagc
    22921 actgaccgac gccaacagga tctgaacgag agtaccccaa ccaaaaaacc acagttgcgc
    22981 tgcgattcct ggcacggctg cgagcaacac caatagcata atgcgcgatg tctggcgctg
    23041 gttatgggta taaggggagc tagctattct gaataccatt tagtcctcgt ttacaacctt
    23101 ttgctgggcg gcttttttgg cctgaacgcg ggcaatagcc gcggcaactg cggc
//
LOCUS       DAJTXY010000058        22639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTXY010000058 DAJTXY010000000
VERSION     DAJTXY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.058"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..194)
                     /locus_tag="OO566_002319"
     CDS             complement(<1..194)
                     /locus_tag="OO566_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR6605584.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKGY"
     gene            complement(335..2368)
                     /gene="metG"
                     /locus_tag="OO566_002320"
     CDS             complement(335..2368)
                     /gene="metG"
                     /locus_tag="OO566_002320"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6605585.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2500..3609
                     /gene="apbC"
                     /locus_tag="OO566_002321"
     CDS             2500..3609
                     /gene="apbC"
                     /locus_tag="OO566_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6605586.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3871..4152
                     /locus_tag="OO566_002322"
     CDS             3871..4152
                     /locus_tag="OO566_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6605587.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4445..4987
                     /locus_tag="OO566_002323"
     CDS             4445..4987
                     /locus_tag="OO566_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6605588.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5068..5742
                     /locus_tag="OO566_002324"
     CDS             5068..5742
                     /locus_tag="OO566_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6605589.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5758..8238
                     /locus_tag="OO566_002325"
     CDS             5758..8238
                     /locus_tag="OO566_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6605590.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8254..9287
                     /locus_tag="OO566_002326"
                     /pseudo
     CDS             8254..9287
                     /locus_tag="OO566_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9369..9707)
                     /gene="rcnB"
                     /locus_tag="OO566_002327"
     CDS             complement(9369..9707)
                     /gene="rcnB"
                     /locus_tag="OO566_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6605591.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9926..10768)
                     /gene="rcnA"
                     /locus_tag="OO566_002328"
     CDS             complement(9926..10768)
                     /gene="rcnA"
                     /locus_tag="OO566_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR6605592.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHEHHQDHEHHHDQGHHHHHEHGEYQDAHA
                     RAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVS
                     FSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFM
                     GIMR"
     gene            10889..11161
                     /gene="rcnR"
                     /locus_tag="OO566_002329"
     CDS             10889..11161
                     /gene="rcnR"
                     /locus_tag="OO566_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6605593.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11253..11349
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11384..12172
                     /gene="thiM"
                     /locus_tag="OO566_002330"
     CDS             11384..12172
                     /gene="thiM"
                     /locus_tag="OO566_002330"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6605594.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12169..12969
                     /gene="thiD"
                     /locus_tag="OO566_002331"
     CDS             12169..12969
                     /gene="thiD"
                     /locus_tag="OO566_002331"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6605595.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13034..13852
                     /locus_tag="OO566_002332"
     CDS             13034..13852
                     /locus_tag="OO566_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6605596.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13904..14650
                     /locus_tag="OO566_002333"
     CDS             13904..14650
                     /locus_tag="OO566_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6605597.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14624..15589)
                     /locus_tag="OO566_002334"
     CDS             complement(14624..15589)
                     /locus_tag="OO566_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6605598.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15586..16589)
                     /locus_tag="OO566_002335"
                     /pseudo
     CDS             complement(15586..16589)
                     /locus_tag="OO566_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16586..17062)
                     /locus_tag="OO566_002336"
                     /pseudo
     CDS             complement(16586..17062)
                     /locus_tag="OO566_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            17216..18268
                     /gene="fbaB"
                     /locus_tag="OO566_002337"
     CDS             17216..18268
                     /gene="fbaB"
                     /locus_tag="OO566_002337"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6605599.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18576..19430
                     /gene="gatY"
                     /locus_tag="OO566_002338"
     CDS             18576..19430
                     /gene="gatY"
                     /locus_tag="OO566_002338"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6605600.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19459..20726
                     /gene="gatZ"
                     /locus_tag="OO566_002339"
                     /pseudo
     CDS             19459..20726
                     /gene="gatZ"
                     /locus_tag="OO566_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            20736..21188
                     /gene="gatA"
                     /locus_tag="OO566_002340"
     CDS             20736..21188
                     /gene="gatA"
                     /locus_tag="OO566_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6605601.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21219..21503
                     /gene="gatB"
                     /locus_tag="OO566_002341"
     CDS             21219..21503
                     /gene="gatB"
                     /locus_tag="OO566_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6605602.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21507..22639
                     /locus_tag="OO566_002342"
                     /pseudo
     CDS             21507..22639
                     /locus_tag="OO566_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
BASE COUNT         5875 a         5256 c         5714 g         5794 t
ORIGIN      
        1 tcatacccct tcttcacctt ctccgcaatc agcttcagtt ccgctttttc cgccgccgca
       61 gcatctgaaa aactttttat ctgactttgc cctttggtgc caacttttcc ccaactgata
      121 tgcaactcgt ttccctgttg ctccaccgtc cagaatttat gtgatttttc gtcctgatag
      181 ataaagtgtc tcattctgca tccctgtaat tttatccagt tgaatgcaga tgctaccagt
      241 atttatacgg gttagagaga gacaaaatcg caagaaaaca actttaggaa tttataaaga
      301 aaaggcgctg tcgatgcagc gccttgaagg gggattattt cacctgatga cccggtttag
      361 caccggcatc cgggcttagc aggaaaatat ctttcccgcc aggaccggca gccatcacca
      421 tgccttcaga gataccgaag cgcattttac gtggtgccag gttagccacc ataatggtgt
      481 gacgaccaat cagtgcctgc ggatccgggt aagcagaacg aataccggag aagacattgc
      541 gtttttcacc gccgagatcc agcgtcaggc gcagcagttt gtcagaacct tcaacaaact
      601 ctgcgttttc gatcagcgcc acgcgcaggt caactttggc gaagtcgtca aaggtgatgg
      661 tttcctgaat cggatcatct gccagtgggc cagtaactgg cgcggtagtg gctttcactt
      721 cttctttaga ggcttccacc agagcttcaa cctgtttcat atcgatgcgg ttatacagcg
      781 ccttgaacgg attcactttg tggcccagca gcggttgctg gataccatcc caggtcagtt
      841 ccgtattgag gaaggcttct gcacgctcgg tcagtttcgg cagtaccggc ttcaggtaag
      901 tcatcagcac gcggaacagg ttgatgccca ttgagcaaat cgcctgcagg tcagcatcgc
      961 gaccttcctg ttttgcgact acccacggag cctgctcgtc gacatagcgg ttagccaggt
     1021 cagccagtgc catgatttcg cgcactgctt taccaaattc acggctttcc cacgcttcac
     1081 caatcacttc agcggcatcg gtaaaggttt tgtacagctc agggtcagcc agctcgcttg
     1141 ccagcacacc gtcaaaacgc ttgttgataa agcccgcatt acgggaggcc aggttaacca
     1201 ctttgttcac gatatcggca ttcacacgct gaacgaaatc ttccaggttg agatcgatat
     1261 catcaatgcg cgaagagagt ttcgcagtgt agtagtaacg caggctgtca gcgtcaaaat
     1321 gattcagcca ggtgctggct ttaataaagg tgccgcgaga cttggacatc ttcgcgccgt
     1381 tcaccgtcac atagccatga acaaacaggt tggtcggctt gcggaagttg ctgccttcca
     1441 gcatggcagg ccagaacagg ctgtggaagt aaacgatatc tttaccgatg aagtggtaca
     1501 gctcggcggt ggagtctttc ttccagtatt catcgaagct tacgctgtcg ccgcgcttgt
     1561 cgcacagatt cttgaaagaa cccatgtagc caatcggtgc gtccagccag acgtagaaat
     1621 atttgcccgg cgcgttcgga atttcaaaac caaagtatgg cgcgtcgcgg gagatatccc
     1681 actgttgcag gccggattca aaccactcct gcattttgtt tgccacctgc tcctgcaacg
     1741 caccgctgcg ggtccatgcc tgcagcattt cgctgaaaga gggcagatca aagaagaagt
     1801 gttcagaatc acgcatcact ggtgtagcac cagaaaccac cgatttcggc tcgatcaatt
     1861 cagtcgggct gtaggtcgcg ccgcagactt cgcagttatc gccgtattga tccggggatt
     1921 tacatttcgg gcaggtgcct ttcacaaaac ggtccggcag gaacatgcct ttttccggat
     1981 cgtacagctg agagatggtg cggtttttaa taaaaccgtt ttctttcagg cgagagtaga
     2041 taagttctga caactggcgg ttctcttcgc tgtgcgtcga gtgatagttg tcatagctga
     2101 tgttaaagcc tgcgaaatca gtctgatgct cctgactcat ttcgccaatc atctgctccg
     2161 gggtgatacc gagctgctga gctttcagca tgatcggtgt accgtgggca tcgtcggcgc
     2221 agatgaagtt gacctcgtgg ccgcgcattc gctggtaacg gacccagaca tcagcctgga
     2281 tgtgctccag catatggccg aggtggattg agccgttagc gtacggcagt gcgcacgtca
     2341 ccagaatttt cttcgcgact tgagtcatag taggcattac ttcttaatag tgaaaagggg
     2401 ttagatagta ccaaatggga aaatgttaag taagtacttc tctatattct tcaggtaaac
     2461 tatcagctga tggtttatct atacaaaaaa ggagtcggga tgaacgaaca atcccaggcc
     2521 aaatcaccag aagccttgcg cgcaatggtc gccgggacac tggccaattt tcagcaccca
     2581 accctgaagc ataacctcac cacgctgaaa gcgttgcacc atgtcgcctg gatggacgac
     2641 acgttgcatg tggaactggt tatgccgttt gtctggcata gcgcgttcga agagttaaaa
     2701 gagcaatgta gcgccgaact gctgcgcatc accggggcga aagctatcga ctggaaactg
     2761 tcgcataaca tagccactct gaaacgcgtg aagaatcagc cgggaattaa cggtgtgaag
     2821 aacattatcg cggtgagttc cggtaaaggc ggcgtgggta aatcgtccac ggcggtaaac
     2881 ctggcgctgg cactggcggc ggaaggggcg aaagtcggca ttctggacgc cgatatctac
     2941 ggcccatcaa ttcccaccat gctgggcgca gaaaatcagc gtccaacttc gccggacggt
     3001 acccacatgg caccgattat gtctcatggc ctggcaacca attcaattgg ttatctggtc
     3061 actgacgaca atgcgatggt gtggcgtgga ccgatggcga gcaaggcgtt aatgcagatg
     3121 ttgcaggaaa ccttgtggcc ggatctcgac tatctggtgc ttgatatgcc gccaggcacc
     3181 ggtgacattc aattgacgct ggcgcaaaac attccagtaa ctggtgcagt cgtggtcacc
     3241 accccgcagg acatcgcgtt gatcgatgcg aaaaaaggca ttgtgatgtt cgagaaagtc
     3301 gaggttccgg tgctcggtat tgtcgagaac atgagtgtgc atatttgcag taactgtggt
     3361 caccatgaac cgattttcgg taccggtggt gcagagaaac tggctgaaaa ataccataca
     3421 cagctgctgg ggcagatgcc gttacacatc tccttacgtg aggatctcga taaaggcacg
     3481 ccaacggtta tcagccgtcc ggagagcgaa tttaccgcta tctaccgcca gctggctgac
     3541 cgcgttgcag ctcagctcta ctggcagggt gaagtcattc ctggcgagat ttccttccgc
     3601 gcggtctaac gcctattaac cattctggtt attcttcata catcgcagag atgttatatt
     3661 gatgcggtgt atttgttttt gccgcattaa tgaacactta tttaatactg cgaatattct
     3721 gcggaccttc gtccgaaact ctacaaagtc gagtgatcgt acaggcaatg cttcattatc
     3781 atccttcgtg ctgtgtagtg gatgtacagc tatagtttgt tatgcactca ttctatccag
     3841 gcataacctt tactacaggg ataacgttca atgaataagt attggctgtc aggaattatt
     3901 tttcttgcct atggattggc ttctcccgca gtctcttcag agactgccac attaacgatt
     3961 aatggcagga tcagtccccc aacatgcagt atggctatgg ttaatagtca accacaacag
     4021 cattgtggtc agttaacgta taatgttgac acccgtcatc aggtttcttc gcccgtcaaa
     4081 ggtgttacga cagaagtggt tgttgcaggt agcgatagca aacgtcgaat cgtgttaaat
     4141 cgctacgatt aatttggcaa cctgatttta attgcattta gaaagacgaa atcatttata
     4201 taaatgattt cgtctttttt attgatatca acaataccat ttacataaat ctacattaat
     4261 taatagatgt gataaatatt taatattctt aagtatttgt tgtatttata ttgcattggt
     4321 taatttgttt taaatcaata aaacaatatg tttacattgc ggcagctttt ttgatgtgag
     4381 aaatttgttt ccaagagata cagactctta acaaccaaaa ataaatttta ttaaggaaca
     4441 tattatgaaa cgttcaatta ttgctgccgc tgtcttttct tcttttttta tgagcgctgg
     4501 agtatttgct gcagacgttg ataccggaac attaaccatt aaaggaaata ttgcagaatc
     4561 tccgtgtaaa ttcgaagcgg gtggtgattc agtaagtatt aatatgccga ctgtaccaac
     4621 cactgtcttt gaaggtaaag ctaaatattc tacctatgat gatgcagtcg gtgtaaccag
     4681 cagcatgtta aaaattagct gcccgaaaga agttgctggt gtaaaactct cgttgatcac
     4741 aaacgacaaa ataaccggta acgataaggc gatagccagt agcaacgata ccgtgggtta
     4801 ctatctctat ttaggtgata acagcgatgt cctagatgtt tctgcacctt ttaacattga
     4861 gagttataaa acagcagaag gtcaatatgc tattccgttt aaagcaaaat acctgaaact
     4921 gacagataac tcagtgcaat caggtgatgt gttatcttct ctggttatgc gtgtagcgca
     4981 ggattaataa ataaaattgc aagtttctcc aggtaataaa catggccgct atccccatgg
     5041 cggcttttta atttaagagg cattaagatg aaaggattat tatctttact cattttttct
     5101 atggtccttc ctgcacatgc cggaattgtt atctacggga cgcgcattat ttacccggca
     5161 gaaaataaag aagttatggt gcagttgatg aaccagggaa atcgttcttc gctgctgcag
     5221 gcgtggattg atgatggcga tacgtcgtta ccaccagaaa aaattcaggt tcctttcatg
     5281 ttaacgccac cagtggcaaa aataggggca aattcagggc agcaagtaaa aatcaaaatt
     5341 atgccgaata aactgcccac taataaagaa agcatttttt atctgaatgt tctggatatt
     5401 ccaccaaata gtccagagca agaaggcaag aatgcactga agtttgcgat gcaaaacaga
     5461 attaagttgt tttaccggcc agcgggtatt gctccggtaa ataaagcgac ctttaaaaag
     5521 ttactggtaa atcgcagtgg caatggattg gtgataaaaa atgactcagc taattgggtg
     5581 acgatttcgg atgtcaaagc taataatgtt aaagtcaatt atgaaactat tatgattgcc
     5641 cccttagaaa gtcagagtgt taatatcaaa agtaataatg caaataactg gtatctgacc
     5701 attatcaatg accacggcaa ctatattagt gacaaaattt aattgcagga gctgcctatg
     5761 ttgagaatga ccccacttgc atcagcaatc gtagcgttat tgctcggcat tgaagcttat
     5821 gcagctgaag aaacctttga tacccatttt atgataggtg gaatgaaaga ccagcaggtt
     5881 gcaaatattc gtcttgatga taatcaaccc ttaccggggc agtatgacat cgatatttat
     5941 gtcaataagc aatggcgcgg gaaatatgag attattgtta aagacaaccc gcaagaaaca
     6001 tgtttatcaa gagaagttat caagcggtta ggcattaata gcgataactt cgccagcggt
     6061 aagcaatgtt taacatttga gcaacttgtt cagggtggga gctatagctg ggatatcggg
     6121 gtttttcgtc tcgatttcag tgtcccgcag gcttgggtgg aagaactgga aagtggctat
     6181 gttccaccgg aaaactggga gcggggtatt aatgcgtttt atacttctta ttatgtgagt
     6241 cagtattaca gcgactataa agcgtcgggt aacaacaaga gtacatatgt acgttttaac
     6301 agcgggttaa atttactgga gtggcaactg cattctgatg ccagtttcag taaaacaaat
     6361 aacaatccag gggtgtggaa aagcaatacc ctgtatctgg aacgtggatt tgcccaattt
     6421 ctcggcacgc ttcgcgtggg tgatatgtac acatcaagcg atatttttga ttctgttcgc
     6481 ttcagcggtg tgcggttgtt tcgtgatatg cagatgttgc ctaactcgaa acaaaatttt
     6541 acgccacggg tgcaggggat tgctcagagt aacgcgttgg taactattga acagaatggt
     6601 tttgtggttt atcagaaaga ggttcctcct ggcccgtttg cgattacaga tttgcagttg
     6661 gccggtggtg gagcagatct tgatgttagc gtgaaagagg cggacggctc ggtaaccacc
     6721 tatctggtgc cttatgcagc ggtgccaaat atgctgcaac ccggcgtgtc gaaatatgat
     6781 tttgcggcgg gccgtagcca tattgaaggg gcgagcaagc aaagtgattt tgtccaggca
     6841 ggttatcagt atggttttaa taacttattg acgctgtatg gcggcacgat ggttgctaat
     6901 aattactatg cgtttaccct cggaacgggt tggaacacgc gcattggcgc aatttccgtc
     6961 gatgccacga agtcgcatag taaacaagac aacggcgatg tgtttgacgg gcaaagttat
     7021 caaattgcct acaacaaatt tgtgagccaa acgtcgacgc gttttggtct ggcggcctgg
     7081 cgttattcgt cgcgtgatta ccggacattt aacgatcacg tttgggcaaa caataaagat
     7141 aattatcgcc gtgatgaaaa cgatatctat gacattgccg attattacca gaacgatttt
     7201 ggccgtaaaa atagcttttc tgccaatatg agccagtcat tgccagaagg ctggggttct
     7261 gtgtccttaa gtacgttatg gcgagattac tgggggcgta gcggcagcag taaggattat
     7321 cagttgagtt attccaataa ctggcggcgg ataagctata ccctcgcggc aagccaggcg
     7381 tatggcgaga atcatcatga agagaaacgt tttaatattt ttatatcaat tccctgtgat
     7441 tggggtgatg acgttacgac gcctcgtcgg caaatatata tgtctaactc aacgacgttt
     7501 gatgatcagg gttttgcctc aaataatacg ggattatcag gaacagtagg gagtcgggat
     7561 cagttcaatt atggtgtcaa cctgagtcat caacatcagg gaaatgaaac gacagctggg
     7621 gcgaatttga cctggaacgc gccggttgcg acagtgaatg gcagttatag tcagtcgagt
     7681 acttatcgac agactggagc cagtgtttca gggggcattg tcgcctggtc gggtggcgtt
     7741 aatctggcga accgtctttc cgaaacgttt gctgtgatga atgcgccagg aattaaagat
     7801 gcttatgtca atgggcaaaa atatcgcaca acaaaccgta atggagtggt ggtatacgac
     7861 ggaatgacac cttatcggga aaatcacctg atgctggatg tgtcgcaaag cgatagcgaa
     7921 gcagaattac gtggcaaccg gaaaattgcc gccccttatc gcggcgcggt tgtactggtt
     7981 aattttgata ccgatcagcg caagccctgg tttataaaag cgttaagagc ggatggtcaa
     8041 ccattaacgt ttggttatga agtcaatgat atccatggtc ataatattgg cgttgtcggc
     8101 cagggaagcc agttatttat tcgcaccaat gaaataccgc catcggttaa tgtagcaatt
     8161 gataagcaac aaggactttc atgcacaatc accttcggta aagagattga tgaaagtaga
     8221 aattatattt gccagtaata gtcaggtggt tctatggaaa ttcgcataat gctatttata
     8281 ttaatgatga tggttatgcc tgtgagctat gcggcatgtt atagtgagtt atctgttcag
     8341 cacaatttgg ttgttcaggg gaattttgca cttactcaaa cacaaatagc gacatatgag
     8401 cataatttta atgattcgtc atgcgtgagt acaaatacta tcacccctat gagcccatcg
     8461 gatattattg ttggacttta taacgatacc attaaattaa atttacattt tgaatggacc
     8521 aataaaaaca acatcacgtt gtcaaataat cagagcagtt tcaccagtgg ttattcggta
     8581 acggtgacac ctgcggccag taatgcaaaa gtgaatattt ctgcgggggg cggtggttca
     8641 gtgatgatta atggtgttgc gacattatcc agtgcttcat catcgacacg cgggagtgcc
     8701 gcagtacaat ttctactgtg tttattaggt ggcaagtcat gggatgcatg tgtaaatagc
     8761 tacagaaatg cattggcaca aaatgcaggt gtctattcct tcaatctgac attgtcatac
     8821 aacccgataa ccacaacctg caaacaggac gatttattaa ttactttaga cagtattccc
     8881 gtttcacaat taccagccac aggtaacaaa gcaacaataa atagtaaaaa aagggatatt
     8941 attctgcgtt gtaaaaattt attaggtcaa caaaatcaaa catcacggaa aatgcaggtg
     9001 tatttatcaa gttctgactt gttaaccaac agcgacacaa tactgaaagg tgcggaagat
     9061 aatgcgtagg atttattctt gaaagtaatg gttcgccagt cacactttta aatatcacta
     9121 acagcagtaa aggatacaca aatttaaaag aaattgcggc aaagtcaaaa cttacagata
     9181 caacggtttc aattccgata acagccagtt actacgtcta cgatacaaac aaagttaaat
     9241 ctggcgcact ggaggcaacc gcattaatca acgtgaaata cgactaataa caagcaagac
     9301 gagcaagtgg ctaataataa aaaataacaa ggtgtctgca tttccccctc atgatgaggg
     9361 ggcttttttt agcgatgata aaaaatctca ccgtcgtagg ctttaatgat tttaccgtcg
     9421 gtgtcgctga tcaacacgta cgcgccaccc atataggtcc agtgcgtccc ggcatcaggc
     9481 gcgggcaggt tacgtaactg ccactgctta atgttgtact catcggtgcg gtacatctct
     9541 gggacggtat cacctggttt gaaatgctta aagtcagcaa tgaaactact taactcatac
     9601 ttagaaattc ccgaggtatt ggtcgaaccc gcggtggctg gtgcggccca ggcggcactg
     9661 gtaaccagca aaagcacacc cagcaacatc ttatttttaa tggtcataaa ctctccactt
     9721 tggggatctg taatatcagt cgcatatctt tcctgccata caatataact gacaagcgat
     9781 attttgttag tgatcccatg atttcgtaaa aagtgtaaat gaaaggatgc cctaaaagca
     9841 tccttagcga gcagagagtc agcggaaata gctgatttaa gaatcatcct gggctgaaga
     9901 taacatgcaa gcctaaagga ttgagttatc gcattatgcc catgaagcca tgtacgccca
     9961 tatacacacc gactaagcca atcaacagac tggaaaaata gggggcgcgt ttagcgagag
    10021 tgttaaatcc gctccagcgt tttgcgacct gctgaacgct gattgctgcg ccaacgccta
    10081 cggtgacaag cgttaacgcc aggccaatgc tgaaactgac gaccagtgtt gcgcccagtg
    10141 tcagggcttt caactgaatg catatcaaca gcacggtaat tgctgccggg caggggataa
    10201 ggccaccggt taagccaaat aacaaaattt gccagttgat gacctctcta ccatcaaagc
    10261 gtcgtttaat gtcattggca tgggctcgtg catgggcatc ctgatactcg ccatgttcgt
    10321 gatggtgatg atgtccttgg tcgtggtgat gttcgtggtc ttgatgatgt tcgtgatgat
    10381 ggtgatgatg ctcataatca tgcccgtgca tattctccag ccagttgcgt tcgccgcgcc
    10441 aggtacgcca gaacatccag aacacggtgc caataatgat cactgcggaa atcagctgga
    10501 gccacggttc tgctgattga gcagtaaagc gcttactgat caccatcccg ccaaaggcaa
    10561 tcaaccagac caccgcggta tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt
    10621 taatggtgcc tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt
    10681 ccaggccatg aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt
    10741 gctgaagaag agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct
    10801 gattctactc ccccccagta cctgatacta ccccccagta gattaatagt gctatgattt
    10861 ttcatattct tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact
    10921 gaaagcgcgt gccagtaaga ttcagggcca ggtcgtggcg ctcaagaaaa tgctcgacga
    10981 gccgcacgaa tgcgctgcag ttttacaaca gattgctgct atccgtggag cggtaaacgg
    11041 tctgatgcgg gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga
    11101 gctaaaacgt gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata
    11161 atttattaac gcgattgtaa aactgccgtt tttcctcgtt tacaacgggt gcgctggaca
    11221 ttaccatcct cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt
    11281 gaaggctgag aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg
    11341 accaccaggt cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct
    11401 gagttcagcg caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca
    11461 ctgcatgacc aatgatgtgg tgcaaacctt taccgccaat accttgctgg cgctcggtgc
    11521 atcgccagcg atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc
    11581 cttgttgatt aacgttggca cactgacgca gccacgcgca caggcgatgc gtgctgccgt
    11641 tgagcaagca aaaagctctc aaacacgctg gacgcttgat ccagtagcgg ttggtgcgct
    11701 cgattatcgc cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg
    11761 taatgcttcg gaaatcatgg cattagctgg cattgctaat ggcggacggg gagtggatac
    11821 cactgacgcc gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc
    11881 aatcgtcgtg gtcactggcg aggtggatta tgttaccgat ggacatcgta tcattggtat
    11941 tcacggtggc gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt
    12001 tgtcgctgcc tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca
    12061 ctggatgaaa caagccggag aacgcgcagt cgccagaagc gaggggccag gcagttttgt
    12121 tccacatttc cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt
    12181 aacgctctga cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt
    12241 aaaaccttct cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa
    12301 aatacccgtg gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc
    12361 gattcggtgt tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc
    12421 gatattgttg aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc
    12481 gacaccgtta tgctggcaaa aagcggcgac ccgctgcttt caccttcggc ggttgctacg
    12541 ctgcgcagtc gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagctgcc
    12601 gccttgctcg acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg
    12661 ctgttggcga tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa
    12721 agcccggact ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg
    12781 accaaaaaca cccacggcac tggttgtaca ctctctgcgg cgttggctgc actacgcccg
    12841 cgccatacaa actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta
    12901 gcccaggccg acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc
    12961 tggtggtgag caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc
    13021 agggatgaaa ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg
    13081 cgctcttggg ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta
    13141 ttcgacagca gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc
    13201 tttcgctatt cgcattcctg ccagttacac cattcacgga ttagatgttt cgcgctggca
    13261 ggagcgtatc gactggcagc gggtggcaaa aatgcgcgac aacgatatcc gcttacagtt
    13321 tgcttttatt aaggctacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg
    13381 gcaattaagc cgcgaaaatg gcctgctgcg cggggcgtat cattatttct ccccgtcggt
    13441 atccgcttca gttcaggcgc gattatttct gcaaacggtg gatttctcac aaggcgattt
    13501 ccctgccgtg ctggacgtag aagaacgggg aaaattatcg gcaaaagaat tacgcaagcg
    13561 ggtaagtcag tggctaaaaa tggtcgaaaa acgcacggga aaaaagccga ttatttactc
    13621 aggagccgtt ttttatcaca ccaatctggc gggctatttc aatgaatatc cgtggtgggt
    13681 ggctcactat tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca
    13741 ttccgaccgt ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg
    13801 cacggtggat gagttgcagg cattcgttga tgggattaaa gaaacgcctt aaataagaag
    13861 cagtgatata ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac
    13921 ccagctgatt gcccaactga acgagcgtat tttagcggcg gataacacgc cgctttatat
    13981 taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat
    14041 tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa
    14101 ggcgatgcag gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca
    14161 gatcaacaac atcttcgaat actcgttgaa agaagcgaga ggtttttctc agcaggtggt
    14221 attgcgcggg aaaaagcccg atacgctatg ggttaataag cgtgtcgtga aatgtcccga
    14281 agaagttgcg cagcagctgg cggtcgaagc gggaagtgat gtctttttgc ttaagcgtat
    14341 tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat
    14401 tcatgatgtt gatgccatcg gaatttctct ttatgactat ttccgcagcc agcacattta
    14461 cccacaacgt acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca
    14521 tattcagtta gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca
    14581 gcaacggcct attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg
    14641 cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg
    14701 acgccactgc attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg
    14761 caagtacgcc acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa
    14821 atgcccgaat gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac
    14881 gaacgatcaa cggcgcggca aatttctcct gccattgctt gccaagtgtt gttatctctg
    14941 cagataaagc aaaacgttcg gcggcaatct cagcctcttg acgattgagt gacactaaag
    15001 gtcgacaggc catgatccgc gccagtaatg cgtccgggat atcgccaata cgtgggccaa
    15061 aatcgataaa cggcgtcacg tcttgcagct tttccagcca ttccactaac aattcgccgc
    15121 agggcgaggc cagttgataa ccggaaaaat agagcaggtt gccaggcgca acggttaatc
    15181 gtgccaacca ttggcgattc cattgatttt ccacaccgct gaatgacata aaagtgcgtt
    15241 cgccatccgg ctcaaccagt gccagacacc agccgttatc gctttcggcg ttatcgatca
    15301 ggctgattaa cccctctttc gccatccggt tgcgaataat ctccgcccac acgccctgac
    15361 cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca
    15421 gtgcgcagcc gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg
    15481 ggagggcata agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct
    15541 gacgcgtggt taattcaggc agcagtgtgt gtaatcgagc gccgctcata ccgcctcccg
    15601 ttgttgacga tatttctcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc
    15661 atccagctcc gcctttaacg caggatcgac agcgttaatg ccatgcaacg cgccgcaaat
    15721 cgccgtcgcc atagcaccta tggtgtctgt gtcgccgcca agattggcgc acaggacggc
    15781 gcagcggttt gggtcggttt gtgccagttc aactagcgca atggcgcacg gaacggactc
    15841 aatagtgctg gtgcctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc
    15901 gtcggcatta cgcacaattt tcagcgcaat ctccagacgt gccgccagtg aggcgctgaa
    15961 cgtggtggtg cgtttttgct gtgcatgtcg cgcaattgaa ggcaatgaat caacaatcgc
    16021 tgaccagcgt tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc
    16081 aaccgccaga tcggatttat gggtcgggct ggacgccagc gccacatcat caataaagga
    16141 atcaacatca cgcgctggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt
    16201 ggttacgccg ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc
    16261 aatcttcgag gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca acgcccagtc
    16321 gagaatatta cgcccaatca gattcggatc gatcttgcct tcacattcca gtaacgcatc
    16381 cgccagacac aggccatcga ggtatcgtcg gtgaattcgg cgcggttaaa ataacaggcc
    16441 gcgttattct cctttggtcc aggcagaaaa cggtcaatcc agccaaagtg tgctttaacg
    16501 cggctgcgtg gccaaagctc ggagggcatc cccatcgcat cccctaacgc ctgcccataa
    16561 agagcaccga gaatacgttc tgttttcatt taacttcccc ttgtgtcaac gcaatatctc
    16621 gatcatcgac cttgatagcc gtaatttcgt tgtcggattc gcgggaaaaa atcatgaaca
    16681 gcacggcgat aatggcaatc atcaccgcgc cgaaagtcca catcccggac cagttgaaag
    16741 tcagtccgtt taccggttcc tgataagcga acatcttttc cgtcatcaca ccgccaagac
    16801 gatagccgag caaactgccg aagccctggc agcagagcgt gatcagcccc tgcgcagcgg
    16861 tacgcatatg cacgggggct tttttatcga catagatgta agcggtaacg tagtaaaaat
    16921 cgtaacttac gccgtgaagc aaaataccga ggaacagtaa cgcgtaggtg aaattgacag
    16981 gtaatgtaag gtacgcgatg acaataccac ataataatac cttttgaata cctttgcggt
    17041 aaaaacttga ccgcgctaac attttttctg atgaatcgag ccaacagaaa acgctgaaaa
    17101 aacatccaaa agatggaaaa actcgcccgt aacgaccagg attatgattg cccgcaaaag
    17161 gcgggccagg acaatccata gccgatatcc aatcggaatt tacgggagca tagtaatgac
    17221 agatattgcg cagttgcttg gcaaagacgc cgacaacctt ttacagcacc gttgtatgac
    17281 tattccttct gaccagcttt atctccccgg acatgactac gtagaccgcg tgatgattga
    17341 caataatcgc ccgccagcgg tgttacgtaa tatgcagacg ttgtacaaca ccgggcgtct
    17401 ggctggcaca ggatatcttt ctattctgcc ggttgaccag ggcgttgagc actctgccgg
    17461 agcttcattt gctgctaacc cgctctactt tgacccgaaa aacattgttg aactggcgat
    17521 cgaagcgggc tgtaactgtg tggcatcaac ttacggcgtg ttggtgtcgg tatcgcggcg
    17581 ctatgcgcat cgcattccat ttctcgtcaa acttaatcac aacgagacgc taagttaccc
    17641 gaatacctac gatcaaacgc tgtatgccag cgtggagcag gccttcaaca tgggcgcggt
    17701 ggcggttggt gcgactatct attttggctc ggaagagtca cgtcgccaga ttgaagaaat
    17761 ttctgcggcc tttgaacgcg cgcacgagct gggcatggtg gcagtgctgt gggcctattt
    17821 gcgaaactcc gcctttaaga aagatggcgt tgattaccat gtttccgccg acctgactgg
    17881 tcaggcgaac catctggcgg caaccatagg tgcagatatc gtcaaacaaa aaatggcgga
    17941 aaataacggc ggctataaag cgattaatta cggttatacc gacgatcgcg tgtacagcaa
    18001 gttaaccagc gaaaacccga ttgatctggt gcgttatcag ttagctaact gctatatggg
    18061 ccgggctggg ttgataaact ccggcggtgc tgcaggcggt gaaactgacc tcagcgatgc
    18121 agtgcgtact gcggttatca acaaacgcgc aggcggaatg gggctgattc ttggacgtaa
    18181 ggcgttcaag aaatcgatgg ctgacggcgt gaaactgatt aatgccgtgc aggacgttta
    18241 tctcgatagc aaaattacta ttgcctgata cgcctcatac gaacagcacc accccgtagg
    18301 acggataagg cgtttacact gcatccggca acctatgcct gatgcgacgc ggaagcgtct
    18361 tatcatgcct acatagcact gccacgtatg tttacaccgc atccggcata aaaacacgcg
    18421 cactttgcta cggcttccct atcgggaggc cgtttttttg cctttcactc ctcgaacaat
    18481 tttcatattg tcgtttttgt gatcgttatc tcgacattta aaagcaaata atttcattat
    18541 tttttgaaat cgaaagcaaa caacaagata tgaaaatgta cgtggtatcg acaaagcaga
    18601 tgctgaacaa cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg
    18661 aaacgatgca agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg
    18721 gaacgcctgg cacatttact catgctggta cagaaaatct gttggcgctg gtcagcgcga
    18781 tggcgaagca atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg
    18841 atatcgctca gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc
    18901 cttttgcgca aaatatttca cgggtcaaag aggtggtgga tttttgccat cgctttgatg
    18961 tcagcgtcga ggcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg
    19021 aagccgatgc gttgtacacc aaccccgcac aggcgcgtga atttgccgag gcaaccggga
    19081 ttgattccct ggcggtcgcc atcggcacgg ctcacggtat gtatgccagc gcaccggtgc
    19141 ttgatttttc tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg
    19201 gcgcgtcagg attatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa
    19261 tcaacgttgc aacggagctg aaaaatgcct tctcgcagtc gttaaaaaat tacctgaccg
    19321 agcaccccga agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg
    19381 atgtggtgag caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc
    19441 atttaacaag gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc
    19501 ggcatatgtt cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat
    19561 cgcaacagca cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc
    19621 ggttataccg gaatgacact ggcagacttt cgcgaatttg tttttacgat tgccgataaa
    19681 gttgggtttg cacgcgaacg cattattctc ggcggcgatc atctggggcc aaactgctgg
    19741 cagcaagaaa atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt
    19801 cgtgctggct tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata
    19861 ccgttagcac cagaaacggt tgcggaacga gctgctgtgc tttgctttgc tttgctgcgg
    19921 aaagtgtggc gacagattgc cagcgtgagc aactgagcta tgtcattggc actgaagttc
    19981 cggttccggg cggtgaggcc agcgccattc agtcagtaca catcacccat gttgaagatg
    20041 ccgccaatac tttacgtacg catcaaaagg cctttattgc ccgtgggctg acagaggcgt
    20101 taacacgtgt gattgccatc gtggtgcagc cgggtgtgga atttgatcac agcaatatta
    20161 tccattatca gccgcaggaa gcgcaggcgc tggcgcaatg gatagaaaac acccgaatgg
    20221 tttatgaagc acattctacc gattaccaga cccggacggc ttattgggaa ttagtccgcg
    20281 atcactttgc aatattgaaa gtcggtcctg cattaacctt tgctttacgc gaggcgatat
    20341 ttgcactggc acaaattgag caggaactta tcgcccccga aaatcgcagc ggttgcctgg
    20401 cggtaattga agaagtgatg ctcgacgaac cgcaatactg gaaaaaatat tatcgcacgg
    20461 gttttaacga ttcattactg gatattcgtt acagcctgtc ggatcgtatt cgttattact
    20521 ggccgcatag tcggattaaa aatagcgttg aaacgatgat ggtgaatctg caaggcgtgg
    20581 acatcccact gggcatgatt agtcagtatc ttcccaaaca atttgaacgc attcagtccg
    20641 gggaattatc agcaatgccg catcagctta ttatgaataa aatttatgat gttttgcgcg
    20701 cctatcgcta cggttgtgcg gaataaggac ggtatatgac taacctgttt gttcgtagcg
    20761 gaatttcttt tgtcgatcgt agcgaagttt taacctatat cggtaatgag atgctcgcga
    20821 aaggtgtggt tcatgatacc tggccacagg cattaattgc cagagaagca gaattcccta
    20881 ccgggataat gcttgagcag cacgccattg caataccgca ttgtgaggcg attcatgcta
    20941 agtcgtcagc catttatctg ttaaggccaa caaataaagt tcattttcag caagcggatg
    21001 atgataacga cgtggcggta tcgttggtta ttgcgttgat tgtggaaaat ccgcagcagc
    21061 aattgaaact tttacgctgt ttatttggca agttacaaca gcccgatatc gtcgagacac
    21121 taattactct tcctgaaact cagttaaagg aatacttcac aaagtatgtt ttagattcag
    21181 acgaataaat ccctctgtaa caataaaaag gactatttat gaaacgcaag attattgtcg
    21241 cttgcggagg cgcggttgcg acctctacga tggcggcgga agaaattaaa gagttgtgtc
    21301 agagtcataa tattcctgtt gaattaatcc agtgtcgggt taatgaaata gaaacctata
    21361 tggatggtgt gcatttgata tgtaccactg ccagggtgga tcgtagtttt ggcgatattc
    21421 cgttagttca cggcatgcct tttgtttctg gtgtcggtat cgaagcatta caaaataaaa
    21481 ttctgactat cttacagggg tgacctatgt tttcagaagt catgcgttat attctcgacc
    21541 tcggccctac agtgatgctg ccggttgtca tcattatttt ttctaaaata ttaggcatga
    21601 aggcaggcga ttgctttaaa gcgggtctgc atatcgggat tggctttgtt ggcattggcc
    21661 ttgtgattgg cttaatgctg gattccattg gtccggcggc gaaagcgatg gcggaaaatt
    21721 tcgacctgaa tctgcatgtg gtcgatgtcg gctggccggg ctcttcacca atgacctgga
    21781 cgtcgcaaat tgcgctggtg gcgattccga ttgcgatatt ggttaacgtg gcgatgttac
    21841 tgacccgtat gacgcgggtg gtaaatgttg atatctggaa tatctggcat atgaccttca
    21901 ccggcagcgt tgctgcacct ggcaaccggt tcatggatga tagggatggc gggtgtggta
    21961 attcacgcgg cgtttgttta taagctcggc gactggtttg cccgcgatac ccgaaatttc
    22021 tttgagctgg aaggtattgc tattccgcac ggtacgtcgg cgtatatggg gccgattgcg
    22081 gtgctggtcg atgctatcat cgagaaaatc ccaggcgtta accgaattaa atttagcgcc
    22141 gacgatattc agcgcaaatt tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg
    22201 gggctgatta tcggcatcct cgcgggttac gatgtcaaag gtgtattgca gctggcggta
    22261 aaaacggcgg cagttatgct gctgatgcca cggatgatta aacccatcat ggatggttta
    22321 acgcccatcg ctaagcaggc ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc
    22381 ctgattggcc tggatcccgc attactgctg gggcatacgg cggtggtatc ggcaagcctg
    22441 atttttattc cgctcaccat tttaattgct gtttgtgttc cgggtaatca ggtactgccg
    22501 tttggcgatc ttgccactat cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga
    22561 aatctgttcc gcaccttaat ctcgggtgtc atcattatga gcatcaccct gtggatcgcg
    22621 acgcaaacta ttggtttgc
//
LOCUS       DAJTXY010000059        22598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTXY010000059 DAJTXY010000000
VERSION     DAJTXY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.059"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..611
                     /locus_tag="OO566_002343"
     CDS             <1..611
                     /locus_tag="OO566_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6605603.1"
                     /translation="PGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSCLNSALYTA
                     SRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDS
                     SGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVVMLFR
                     PAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            632..1290
                     /gene="csiR"
                     /locus_tag="OO566_002344"
                     /pseudo
     CDS             632..1290
                     /gene="csiR"
                     /locus_tag="OO566_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1291..1740)
                     /gene="kbp"
                     /locus_tag="OO566_002345"
     CDS             complement(1291..1740)
                     /gene="kbp"
                     /locus_tag="OO566_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6605604.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1824..1982)
                     /locus_tag="OO566_002346"
     CDS             complement(1824..1982)
                     /locus_tag="OO566_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6605605.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2165..2464
                     /locus_tag="OO566_002347"
     CDS             2165..2464
                     /locus_tag="OO566_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6605606.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2474..2998
                     /gene="ygaP"
                     /locus_tag="OO566_002348"
     CDS             2474..2998
                     /gene="ygaP"
                     /locus_tag="OO566_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6605607.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3045..3449)
                     /gene="stpA"
                     /locus_tag="OO566_002349"
     CDS             complement(3045..3449)
                     /gene="stpA"
                     /locus_tag="OO566_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6605608.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4117..4554
                     /gene="alaE"
                     /locus_tag="OO566_002350"
     CDS             4117..4554
                     /gene="alaE"
                     /locus_tag="OO566_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6605609.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4591..4941)
                     /locus_tag="OO566_002351"
     CDS             complement(4591..4941)
                     /locus_tag="OO566_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6605610.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
     gene            5093..5422
                     /locus_tag="OO566_002352"
     CDS             5093..5422
                     /locus_tag="OO566_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6605611.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     QLSMRKS"
     gene            5670..5915
                     /gene="nrdH"
                     /locus_tag="OO566_002353"
     CDS             5670..5915
                     /gene="nrdH"
                     /locus_tag="OO566_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6605612.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            5912..6322
                     /gene="nrdI"
                     /locus_tag="OO566_002354"
     CDS             5912..6322
                     /gene="nrdI"
                     /locus_tag="OO566_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6605613.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            6295..8439
                     /gene="nrdE"
                     /locus_tag="OO566_002355"
     CDS             6295..8439
                     /gene="nrdE"
                     /locus_tag="OO566_002355"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6605614.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            8449..9408
                     /gene="nrdF"
                     /locus_tag="OO566_002356"
     CDS             8449..9408
                     /gene="nrdF"
                     /locus_tag="OO566_002356"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6605615.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            9763..10965
                     /gene="proV"
                     /locus_tag="OO566_002357"
     CDS             9763..10965
                     /gene="proV"
                     /locus_tag="OO566_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6605616.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            10958..12022
                     /gene="proW"
                     /locus_tag="OO566_002358"
     CDS             10958..12022
                     /gene="proW"
                     /locus_tag="OO566_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6605617.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            12080..13072
                     /gene="proX"
                     /locus_tag="OO566_002359"
     CDS             12080..13072
                     /gene="proX"
                     /locus_tag="OO566_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6605618.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            13264..14443
                     /locus_tag="OO566_002360"
                     /pseudo
     CDS             13264..14443
                     /locus_tag="OO566_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14567..15304
                     /gene="ygaZ"
                     /locus_tag="OO566_002361"
     CDS             14567..15304
                     /gene="ygaZ"
                     /locus_tag="OO566_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6605619.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            15294..15629
                     /gene="ygaH"
                     /locus_tag="OO566_002362"
     CDS             15294..15629
                     /gene="ygaH"
                     /locus_tag="OO566_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6605620.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            15720..16250
                     /gene="emrR"
                     /locus_tag="OO566_002363"
     CDS             15720..16250
                     /gene="emrR"
                     /locus_tag="OO566_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6605621.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            16377..17549
                     /gene="emrA"
                     /locus_tag="OO566_002364"
                     /pseudo
     CDS             16377..17549
                     /gene="emrA"
                     /locus_tag="OO566_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            17566..19104
                     /gene="emrB"
                     /locus_tag="OO566_002365"
     CDS             17566..19104
                     /gene="emrB"
                     /locus_tag="OO566_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6605622.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(19168..19683)
                     /gene="luxS"
                     /locus_tag="OO566_002366"
     CDS             complement(19168..19683)
                     /gene="luxS"
                     /locus_tag="OO566_002366"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6605623.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(19833..21389)
                     /gene="gshA"
                     /locus_tag="OO566_002367"
     CDS             complement(19833..21389)
                     /gene="gshA"
                     /locus_tag="OO566_002367"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6605624.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(21462..21890)
                     /locus_tag="OO566_002368"
                     /pseudo
     CDS             complement(21462..21890)
                     /locus_tag="OO566_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
     gene            complement(21887..22453)
                     /gene="yqaB"
                     /locus_tag="OO566_002369"
     CDS             complement(21887..22453)
                     /gene="yqaB"
                     /locus_tag="OO566_002369"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6605625.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         5436 a         5758 c         5664 g         5740 t
ORIGIN      
        1 tgccggggct gaaagccgtt ggatcttatc gctcggttct ggaattgctc aatattcccc
       61 atgcgaaatt aatcatggac tgtgtgatat tactttccgt aaccagttgc ctgaactcgg
      121 ccctgtatac cgcctcaagg atgctctact ccttaagccg tcgcggtgat gctcccgcgg
      181 taatgggtaa aatcaaccgc agtaaaaccc cgtacgtggc ggtgttactc tccaccggcg
      241 cagcattctt aacggtggtg gtgaactatt acgcgcctgc gaaggtattt aaatttctga
      301 tcgacagctc cggcgctatc gccctgctgg tttatttagt catcgccgtt tcacagttgc
      361 ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg cttgcgaatg tggctttatc
      421 cctggctcac ctggctggtc atcggcttta ttacctttgt gttggtagtg atgctattcc
      481 gtccggcgca acagttagaa gtgatctcca ccggcttatt agcgataggg attatctgta
      541 ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg gcaaaaaaca cccgttcata
      601 atacgcgctg ataatgatca ggagtcacac catgaccatt acgtctctgg atggctatcg
      661 ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat tacggatgag
      721 tttactgaca tcgcgttatg cacttggcgt tgggccgttg cgggaagcac tttctcaact
      781 ggtggcggaa cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg cgtctatgtc
      841 agagcaggag ctgctctgac atagacgcca cccgatatgg aagcgatgct ggtcagtctg
      901 gcgattgccc gcggtggcga tgaatgggag gcagacgttc tcgcaaaagc gcatctgctc
      961 aataagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga tctgcgtcat
     1021 caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct gcaaatgcgt
     1081 gaacggttgt ttgatctggc ggcgcgttat cgatttatct ggctgcggcg aacggtgctt
     1141 tcggtggaaa tgctggagga taaacacgat cagcaccaga ccctgactgc ggcggtactg
     1201 gcgcgagata cagcgcgcgt cagtgagtta atgcgccagc atttactgac gccaattccc
     1261 attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca acacttgccc
     1321 cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga ttttattgta
     1381 cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc cagacttaac
     1441 ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat caacactggc
     1501 aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct gactcaggcc
     1561 gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat cggcatcagg
     1621 tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg
     1681 acctgtaacc gcatcccaga gtttttctcc ggcatctttc acaaaattga acagacccat
     1741 agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg cggcgcgtta
     1801 acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa
     1861 ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca accgaaccct
     1921 ttaccgagca gcacgccgag cggcggcaga atgatggtga tgacgattct ccagaaaccc
     1981 atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat ctcttaagcg
     2041 tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat ttacgctgct
     2101 gaggctggta tatgttgcat ttttagttta gagtttactt aatttagaaa atacttaaat
     2161 aaatatgact gaactcgcgc aattacaggc cagtaccgaa caggcagcgg ccttattgaa
     2221 agcaatgagc caccctaaac ggttgctgat tctgtgcatg cttagcggtt cccccggcac
     2281 cagcgcggga gagctgacgc gcattaccgg actgagcgcc tctgcgacat cacagcatct
     2341 cgctcgtatg cgggacgaag ggcttatcaa cagccaacgg gatgcccaac gcattctata
     2401 ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg tctattgtcc
     2461 gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag aattaatcgc
     2521 acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg aacatattcc
     2581 tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtcttccac ctaaattacg
     2641 tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata acgccgataa
     2701 attagcagcg attgccgccc ccgcagaaat atttttactc gaagatggga ttgatggctg
     2761 gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt taatgcgcca
     2821 ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt ataccgtaaa
     2881 tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg caggaatcag
     2941 cggtttttgt ggaatggcaa ggttgttaga taagataccg tggaaccaac gagcttgaga
     3001 agcgacgccg gacgcgccct agcagcgaca tccggcctca gtaattagat caggaaatcg
     3061 tcgagagatt taccttctgc cagcgcctga gcaattggct tcggtgtacg gccctgaccg
     3121 gtccaggttt tagtttcacc gttaacatca gtgaatttat atttcgccgg acgcggctgg
     3181 cgttttttac cagcacgtgg tgcagcagca gagctattac ccaataactc ttccgggtta
     3241 attccgtcag ctttcatcag ctccagccag gtgctaattt tttcctgacg ctcagccagt
     3301 tcacgctgct gctgttcttc ttcttcacgt ctttctttag tgacaaccct gaatttttcg
     3361 agcatttctt caagaacgtc aatggagaat tcgcgagcca tcgcacggag ggtgcgaatg
     3421 ttatttaaac tttgtaacat tacggacata ataaagaaaa ccttttaacg ccaaaacaaa
     3481 aaagtatttc gtcgcgtagt gtaagcgatc acttattata tttccacaaa atttaatcta
     3541 tttacagtcc tgcgcgagat tatttcagct gttattttct gaaactctca gaataatttc
     3601 aagattatat attagccatt ttgatgagta ttttaggtta ttggtgaaca gagtggcgct
     3661 ctctgctcgc taactccgta agcctgacaa aaccgaagtg aataaaaatg caatataaca
     3721 gattaaaaga aaacacccct cactggtgct ttctgctgtt aatgtacatt ttctggcatc
     3781 aaaaatgctg taaatgaccc ggtcacgaca aacaccgaat tctctacgct tatagcgtta
     3841 cctccccccc aaactttcat tagtgaaatt aaaggcaagg ccatatccaa ttgatttaat
     3901 tgaaaataaa tcatatcggc atgtaaaaat ccacaatgta caaaaaacga accgttacgg
     3961 aatattttat ctacaaaagc tgactaaata aaaatttttc actaatttat tagtcatagc
     4021 cagcgatata cgctatgcga aaatacagat ggcaatgaga tccactgctt tcatctccat
     4081 taacatccca ttacgctttt attaaggagc attagcatgt tctcaccgca gtcacgcttg
     4141 cgtcatgcag ttgcagatac gttcgcgatg gttgtttact gttctgtcgt gaacatgtgt
     4201 attgaagttt tcctctccgg aatgagcttc gaacagtctt tttattccag attggtagcg
     4261 attccggtga acatcttaat tgcatggcca tacggtatgt accgcgatct gtttatgcgc
     4321 gcggcacgca aaggctggat aaaaaatctg gctgatatcc tggcttatgt gacgttccag
     4381 tcaccggtgt atgtggcgat cttgttagtg gtgggcgcag actggcatca gattatggcg
     4441 gcggtcagtt caaacatcgt tgtttcgatg ttgatggggg cggtttatgg ctacttcctc
     4501 gattattgcc gccgactgtt taaagtcagc cgttaccagc aggtaaaagc ctgactcttc
     4561 ttttcgcgac tggcatcgcc agtcgcgtct ttaactggct tcgccaaata aaccattcaa
     4621 ataacgttca agcgcaacac gcgaactaaa gccatgcggg ataccgtaat gctcgtgtcg
     4681 ctgtcgctcg cctgctaaat agagcggaaa ttgctggtaa tgatcgcaag ttttaatatc
     4741 ggaagcgggt tcagccagtg ttgaactgcg ttcttttaat gttggatgaa ttaccagagc
     4801 ggtacgtccc attcgcgctt cgcgattaac atagacataa ttttccccac ggcggtaacc
     4861 atacgctttc tgggttacca catcgacagt aaaaccgact ttttcaagta cgcgcgccac
     4921 ctcgtctggt cgtaaataca tatttgatcc tcgttatttt caaccgcggg cctaccttac
     4981 ctgattgcgc attcacaacg ctttcagaaa agtccataaa gccgcgatga acctcagtta
     5041 agaaaattat ggtctacact gaaaattaca tcgaattcta atgggggatc atatgtttaa
     5101 ccgaccgaac cgcaacgacg ttgatgatgg cgtgcaggat atccagaatg atgtcaatca
     5161 attagctgac agcctcgaat ctgtattgaa gtcctggggc agcgacgcca aaggggaagc
     5221 tgaagccgca cgcagtaaag ctcaggcgtt gctgaaagaa accagggcgc gaatgcatgg
     5281 tcgtactcgc gtccagcaag ccgcgcgcga tgccgttggc tgcgcggatt cttttgttcg
     5341 tgaaagaccc tggtgtagcg tgggtacagc agctgcggta ggtattttta tcggcgcaca
     5401 gttaagcatg cgcaaatcgt agtgcaaaaa tgataataaa tacgcgtctt tgaccccgaa
     5461 gcctgtcttc ggggtttctt tttgcctggt gaatcacaaa aatccccctc ccccgtcacg
     5521 ctcatatcca gggtaatttc gatcactatt tgctatatat tgtgtggttg aatctttttt
     5581 caactacatc tagtatctct gtatcaacag agagacaacc cgacgcgtat catcgcgccg
     5641 tatcttcatt ttaaacagaa atacgaatca tgcgcataac tatttatact cgtaacgatt
     5701 gcgttcagtg ccacgccacc aaacgggcga tggaaaaccg gggctttgat tttgaaatga
     5761 ttaatgtcga tcgcgttcct gaagcagcgg aagcgttgcg tgctcagggc tttcgtcagt
     5821 tgccggtagt gattgctggc gatcttagct ggtctggttt ccgtccggac atgattaacc
     5881 gtctgcatcc agcgccacac gcggccagtg catgagccag ctcgtctact tctccagcag
     5941 ctccgaaaac acgcagcgtt ttatcgaacg tttaggtctg cccgcggtgc gcatcccgct
     6001 caatgagcgg gaacggattc aggtagacga gccttacatc ctgatcgtgc cctcttacgg
     6061 cggcggcggt acggctggcg cggtgccacg acaggtaatt cgctttttaa acgacgagca
     6121 caaccgggcg ttgcttcgcg gcgttattgc ttctggtaat cgcaactttg gtgaggcgta
     6181 tggccgcgcc ggagatgtga ttgcccggaa atgcggcgtg ccgtggctgt accgttttga
     6241 actcatgggt acgcaaagcg atatcgaaaa cgttcgtaaa ggagtaaccg aattttggca
     6301 acgacaaccg cagaatgcct gacgcaggaa acgatggatt accacgcgct gaatgcgatg
     6361 cttaatcttt acgatagcgc aggtcgcatt cagttcgata aagaccgcca ggccgttgac
     6421 gcctttattg cgacgcatgt gcgtccgaac agtgtgacct tcagtagcca gcagcagcgc
     6481 ctgaactggc tggtcaacga aggttactat gatgaaagcg ttcttaatcg ctactctcgc
     6541 gactttgtca ttacgctgtt tgcccacgca cacaccagcg gttttcgttt ccagacattc
     6601 ctcggggcat ggaagtttta caccagctat acgttgaaga cattcgacgg taaacgttat
     6661 ctggaagatt ttgccgatcg agtcacgatg gtggcgctga cgctggcaca aggcgatgag
     6721 acgctggcgt tgcaactgac cgatgaaatg ctgtcaggac gctttcagcc agccacgcca
     6781 acattcctca actgcggtaa gcagcagcgc ggcgaactgg tttcctgttt tttgctgcgt
     6841 attgaagaca atatggagtc gattggtcgg gcggtaaatt ccgcactgca gctgtcgaaa
     6901 cgcggcggcg gcgtagcatt tttgctgtcg aatctgcggg aagcgggcgc gccaattaaa
     6961 cgtattgaaa atcaatcttc tggcgtaatt ccggtgatga aaatgttgga agacgcattt
     7021 tcctatgcca accaactcgg cgcgcgtcag ggggcaggtg cagtctattt acatgctcat
     7081 catcccgata ttctgcgttt tctcgacacg aaacgggaaa atgccgacga aaaaatccgc
     7141 attaaaacac tgtcgctggg cgtggtgatc ccggatatca ctttccatct ggcaaaagag
     7201 aatgcgcaga tggcgctgtt ttcgccctat gacgttgagc gagtttatgg caagccgttt
     7261 gccgatatcg ccattagcga acactatgac gaactggttg ccgatgaacg catccgcaaa
     7321 aaatacctca acgcccgtga tttcttccag cgactggcag aaatccagtt tgagtccggc
     7381 tatccctaca tcatgtatga agacacagta aaccgtgcta accctatcgc cgggcgcata
     7441 aatatgagta atctctgctc agaaattttg caggttaaca gcgcctcaga gtatgacgag
     7501 aatctcgact atgcccgcac aggccatgat atttcctgca atttaggttc gttgaatatt
     7561 gcgcacacca tggattcccc cgatttttcc cgcacggtag agactgccgt gcgcggttta
     7621 acggcggtgt cagatatgag tcatatccgc agcgtgccgt ccatcgaagc cggaaatgcc
     7681 gcctcgcacg ccatcggact ggggcagatg aatttacatg gttatctggc gagggaaggc
     7741 atcgcttatg gttcgccgga agccctggat ttcaccaatc tctatttcta taccatcacc
     7801 tggcacgcac tgcgtacctc gatgttgctg gcgcgcgaac gcggtgaaac cttcgccggg
     7861 ttcaaacagt cacgctatgc cagtggtgaa tatttcagcc aatatctgca aggtaactgg
     7921 cagccgaaaa cggcgaaagt tggcgaactg tttgcccgca gcggtattac gttacctacc
     7981 cgtgagatgt gggcgcaact gcgcgacgac gtgatgcgct acggcatata caaccagaat
     8041 ctccaggcgg tgccgccaac cgggtctatc tcttatatca accatgctac gtcgagtatt
     8101 catccgattg tggcgaaagt agagatacgc aaagagggca aaacaggacg cgtttactac
     8161 cctgccccgt ttatgactaa cgaaaatctg gcgctgtatc aggacgctta cgaaattggc
     8221 gcagaaaaga tcatcgacac ctacgcggaa gcgactcgcc atgtcgatca ggggctgtcg
     8281 ctgacgctgt ttttccccga taccgccacc actcgcgata tcaacaaagc gcagatttac
     8341 gcctggcgca aaggtatcaa aacgctctat tacatccgcc tgcgtcagat ggcgctggaa
     8401 ggcactgaaa ttgaaggctg cgtctcctgt gcactttaag gaatatctat gaaactctca
     8461 cgtatcagcg ccatcaactg gaacaagata tctgacgata aagatctgga ggtgtggaat
     8521 cgcctgacca gcaatttctg gctaccagaa aaggtgccgc tgtcgaacga tattcccgcc
     8581 tggcagacat taactgtcgt agaacaacaa ctgacgatgc gcgtttttac tggcctgacg
     8641 ctgctcgaca cgctgcaaaa tgttatcggc gcgccttcgc tgatgcccga tgcgctcacg
     8701 cctcatgaag aggcggtatt atcgaatatc agctttatgg aagcggttca tgcccgctct
     8761 tacagttcga ttttctcgac gctgtgccag accaaagatg tcgatgccgc ctacgcctgg
     8821 agtgaagaaa acgcaccgtt gcagcgaaaa gctcagatta ttcagcaaca ttatcgcggt
     8881 gatgatccgc tgaaaaagaa aatcgccagt gtgtttcttg aatctttttt gttctattcc
     8941 ggtttctggc tgccgatgta tttttccagc cgcggaaagc tgaccaatac cgcggacctg
     9001 atccgtctga ttatccgcga tgaagcagtc cacggttact acataggcta taaatatcag
     9061 aaaaacatgg aaaagatatc tctgggacaa cgtgaagagt tgaagagttt cgccttcgat
     9121 ttgttgctgg aactctacga caacgagttg caatacaccg atgagctgta cgccgaaacc
     9181 ccgtgggctg acgatgtgaa agcgtttctc tgttacaaca ccaataaggc tttgatgaat
     9241 ctgggctacg aaccgttatt tcccgcagaa atggcggaag tgaatccggc aatcctcgcc
     9301 gcgctttcgc cgaatgccga tgaaaatcac gatttctttt ccggttcagg ctcctcttat
     9361 gtgatgggga aagcggttga aacagaagat gaagactgga atttctgagg gtgttatttt
     9421 caaaaatatc actacccgca gcagggaaat aattcccgcc aaatagcttt ttatcacgca
     9481 aatattctgt agtgatctac actgatactc tgttgcatta ttcgcctgaa actacaatat
     9541 tcaggcgttt tttcgctatc tatgacaaaa aatatcaact tttctcgatt tgctctcagc
     9601 ccttatatca cggaaaattc cggcgatttg ctcgcatcaa tattcatgcc acatttgccc
     9661 tcaggggttg cctcagattc tcagtatgtt agggtagaaa aaagtgacta ttcccattgg
     9721 gtaatatatc gacataaaca aataaaggaa tcattctatt gcatggccat taaattagaa
     9781 attaaaaatc tttataaaat atttggtgag catccacagc gagcgttcaa atatatcgaa
     9841 caaggacttt caaaagaaca aattctggaa aaaactgggc tatcgcttgg cgtaaaagac
     9901 gccagtctgg ccattgaaga aggcgagata tttgtcatca tgggattatc cggctcgggt
     9961 aaatccacaa tggtacgcct tctcaatcgc ctgattgaac ccacccgcgg gcaagtgctg
    10021 attgatggtg tggatattgc caaaatatcc gacgccgaac tccgtgaggt gcgcagaaaa
    10081 aagattgcga tggtcttcca gtcctttgcc ttaatgccgc atatgaccgt gctggacaat
    10141 actgcgttcg gtatggaatt ggccggaatt aatgccgaag aacgccggga aaaagccctt
    10201 gatgcactgc gtcaggtcgg gctggaaaat tatgcccaca gctacccgga tgaactctct
    10261 ggcgggatgc gtcaacgtgt gggattagcc cgcgcgttag cgattaatcc ggatatatta
    10321 ttaatggacg aagccttctc ggcgctcgat ccattaattc gcaccgagat gcaggatgag
    10381 ctggtaaaat tacaggcgaa acatcagcgc accattgtct ttatttccca cgatcttgat
    10441 gaagccatgc gtattggcga ccgaattgcc attatgcaaa atggtgaagt ggtacaggtc
    10501 ggcacaccgg atgaaattct caataatccg gcgaatgatt atgtccgtac cttcttccgt
    10561 ggcgttgata ttagtcaggt gttcagtgcg aaagatattg cccgccggac accgaatggc
    10621 ttaattcgta aaactcctgg cttcggccca cgttcggcac tgaaattatt gcaggatgaa
    10681 gatcgcgaat atggctacgt tatcgaacgc ggtaataagt ttgtcggcgc agtctccatc
    10741 gattcgctta aaaccgcgtt aacgcagcag caaggtcttg atgcggcgct gattgatgcg
    10801 ccgttagcag tcgatgcaca aacgcctctt agcgagttgc tctctcatgt cggacaggca
    10861 ccctgtgcgg tgcccgtggt cgacgaggac caacagtatg tcggcatcat ttcgaaagga
    10921 atgctgctgc gcgctttaga tcgtgagggg gtaaataatg gctgatcaaa ataatccgtg
    10981 ggataccacg ccagcggcgg acagtgccgc gcaatccgca gacgcctggg ggacaccgac
    11041 gactgcaccg gctgacggcg gtggtgctga ctggctgacc agtacgcctg cgccaaacgt
    11101 cgagcatttt aatattctcg atccgttcca taaaacgctg atcccgctcg acagttgggt
    11161 cactgaaggg atcgactggg ttgttaccca tttccgtccc gtcttccagg gcgtgcgcgt
    11221 tccggttgat tatatcctca acggtttcca gcaattgctg ctgggtatgc cagcgccagt
    11281 ggcgattatc gttttcgctc tcatcgcctg gcagatttcc ggggtcggaa tgggcgtggc
    11341 gacgctggtc tcgctgattg ccatcggcgc aatcggtgcc tgttcgcagg caatggtgac
    11401 tctggcgctg gtgttaactg ccctgctgtt ctgtatcgtc atcggtttgc cgttggggat
    11461 atggctggcg agaagtccgc gagcggcgaa aattattcgt ccactgcttg atgccatgca
    11521 gaccacgcca gcgtttgttt atctggtgcc aatcgtcatg ctatttggta tcggtaacgt
    11581 gccgggcgtg gtggtgacga tcatctttgc gctgccgccg attatccgtc tgaccattct
    11641 ggggattaac caggttccgg cggatctgat tgaagcctcg cgctcattcg gtgccagccc
    11701 gcgccagatg ctgttcaaag ttcagttacc gctggcgatg ccgaccatta tggcgggcgt
    11761 taaccagacg ctgatgctgg ccctttctat ggtggtcatt gcctcgatga ttgccgtcgg
    11821 cgggctgggt cagatggtac ttcgcggtat cggtcgtctg gatatggggc ttgccaccgt
    11881 tggcggcgtc gggattgtga tcctcgccat tatcctcgat cgcctgacgc aggccgttgg
    11941 gcgcgactca cgcagtcgcg gcaaccgtcg ctggtacacc actggccctg ttggtctgct
    12001 gacccgccca ttcattaagt aactctgcac ttgcccggtg acgccgggca ttatcaccct
    12061 gccaaaaaaa ggaataacaa tgcgacatag cgtacttttt gcgacagcgt ttgccacgct
    12121 tatctctaca caaacttttg ctgccgatct gccgggcaaa ggcattactg ttaatccagt
    12181 tcagagcacc atcactgaag aaaccttcca gacgctgctg gtcagtcgtg cgctggagaa
    12241 attaggttat accgtcaaca aacccagcga agtggattac aacgttggct acacctcgct
    12301 tgcttccggc gatgcaacct tcaccgccgt gaactggacg ccactccatg acaacatgta
    12361 cgaagctgcc ggtggcgata agaaatttta tcgtgaaggg gtatttgtta acggcgcagc
    12421 acagggttac ctgatcgata agaaaaccgc cgaccaatac aaaatcacca acatcgcaca
    12481 actgaaagat ccgaagatcg ccaaactgtt cgataccaac ggcgacggta aagcggattt
    12541 aaccggttgt aaccctggct ggggctgcga aggtgcgatc aaccaccagc ttgccgcgta
    12601 tgaactgacc aacaccgtga cgcataatca ggggaactac gcagcgatga tggccgacac
    12661 catcagtcgc tacaaagagg gcaaaccggt gttttattac acctggacgc cgtactgggt
    12721 gagtaatgag ctgaagccag ggaaagatgt ggtctggttg caggtgccgt tctccgcact
    12781 gccgggcgat aaaaatgccg ataccaaact gccgaatggc gcgaattacg gcttcccggt
    12841 cagcaccatg catatcgttg ccaacaaagc ctggaccgag aaaaatccgg cggcagccaa
    12901 actgtttgcc attatgcagt tgccagtggc cgatattaac gcccagaacg ccattatgca
    12961 tgacggcaaa gcctcagaag gcgatattca gggccacgtt gatggctgga tcaaagccca
    13021 ccagcagcag ttcgatggct gggtgaatga ggcgctggca gcgcagaagt aatttttatt
    13081 cgggcggata aggcgttttc gcctcatccg ccgctctgta cacaatgcct gatgcgacgc
    13141 tgacgcgtcc tatcatgcct acaaaccaca tcgcacattt caacaagcta tctttcatcc
    13201 catattcatc aacatccgct attattgatt tccagcttaa tcatcacctg atgaacaaaa
    13261 ataatgacta aacctaatca tgagcttagc ccggcgctga tcgtgctgat gtctatcgcc
    13321 accggtctgg cggtagccag taactattac gcccagccat tgctcgacac catcgcgcgt
    13381 aacttttccc tttccgccag ttcggcaggc tttattgtta ccgccgcgca gttgggctat
    13441 gccgcaggtc tactgtttct tgttcccctc ggtgatatgt ttgaacgccg ccgcctgatt
    13501 gtctcgatga ccttactggc agcgggcggt atgttaatca ctgccagcag tcagtcactg
    13561 gcgatgatga tcctcggtac ggcattaacc ggtttattct cagtcgtggc acaaattctg
    13621 gttccgctgg cagcgacgct ggcttcaccg gataaacgcg gcaaagtggt tggcaccatt
    13681 atgagcggtc tgctgttggg gatattgctg gcacgaaccg ttgccgggtt actggcaaac
    13741 ctcggcggct ggcgcaccgt cttttgggtt gcttcggtgt taatggcact gatggcgctg
    13801 gcattatggc gtggtctgcc acaaatgaaa tcagaaaccc acctcaacta cccacagttg
    13861 ttgggttccg ttttcagcat gttcatcagc gataagatcc tgcgcacccg cgcgttgctg
    13921 ggctgcatga cctttgccaa cttcagcatt ctctggacct caatggcctt tttgcttgcc
    13981 gctccacctt ttaactacag cgatggtgac attggtctgt ttgggcttgc gggagctgcc
    14041 ggagcgttgg gcgctcgtcc ggcgggcggt tttgccgaca agggcaaatc gcaccacaac
    14101 tttcggtctg cttctgctgt tactttcatg gctggcaatc tggtttggtc acacttccgt
    14161 actggcgttg attatcggca ttctggtgct ggacctcacc gtacagggcg tgcatatcac
    14221 taaccagacg gtaatttatc gaatacatcc tgatgcgcgt aatcgcctga ccgcaggtta
    14281 catgaccagc tactttattg gcggtgccgc cggttcgcta atttcagcct cagcctggca
    14341 acatggcggt tgggctggcg tttgtctggc tggcgcgacg attgccctgg ttaacttact
    14401 ggtctggtgg cgaggttttc atcgtcagga agccgcaaat taagcaatca gcaaccgttt
    14461 tcgtggcttt acacttataa gggtgttaag aagcccatca gtctgataag gttaagatat
    14521 tcattcagtc tatttataat attaacaatc gttaagcgta cactctatgg aaagccctac
    14581 tccacagcct gctcctggtt cggcgacctt catggaagga tgcaaagaca gtttaccgat
    14641 tgttattagt tatattccgg tggcctttgc gttcggtctg aatgcgaccc gtctgggatt
    14701 ctctcctctc gaaagcgttt ttttctcctg catcatttat gcaggcgcga gccagttcgt
    14761 cattaccgcg atgctggcag ccgggagtag tttgtgggtt gctgcactga ccgtcatggc
    14821 aatggatgtt cgccatgttt tgtatggccc gtcactgcgt agccgtatta ttcagcgtct
    14881 gcaaaaatcg aaaactgccc tatgggcgtt tggcctgacg gatgaggttt ttgccgccgc
    14941 caccgcaaaa ctggtacgca ataatcgccg ctggagcgag aactggatga tcggcattgc
    15001 cttcagttca tggtcatcgt gggtatttgg tacggtaata ggggcattct ccggcagcgg
    15061 cttgctgcaa ggttatcccg ccgttgaagc tgcattaggt tttatgcttc cggcactctt
    15121 tatgagtttc ctgctcgcct ctttccagcg caaacaatct ctttgcgtta ccgcagcttt
    15181 agttggtgcc cttgcaggcg taacgctatt ttctattccc gtcgccattc tggcaggcat
    15241 tgtctgtggc tgcctcactg cgttaatcca ggcattctgg caaggagcgc ccgatgagct
    15301 atgaggttct gctgcttggg ttactggttg gcgcggcgaa ttattgcttc cgctatttgc
    15361 cgctgcgcct gcgtgtgggt aatgcccgcc caaccaaacg tggcgcggta ggtattttgc
    15421 tcgacaccat tggcatcgcc tcgatatgcg ctctgctggt tgtctctacc gcaccagaag
    15481 tgatgcacga tacacgccgt ttcgtgccca cgctggtcgg cttcgcggta ctgggtgcca
    15541 gtttctataa aacacgcagc attatcatcc caacactgct tagtgcgctg gcctatgggc
    15601 tcgcctggaa agtgatggcg attatataac ccacaagaat catttttcta aaacaataca
    15661 tttactttat ttgtcactgt cgttactata tcggctgaaa ttaatgaggt catacccaaa
    15721 tggatagttc gtttacgccc attgaacaaa tgctaaaatt tcgcgccagc cgccacgaag
    15781 attttcctta tcaggagatc cttctgactc gtctttgcat gcacatgcaa agcaagctgc
    15841 tggagaaccg caataaaatg ctgaaggctc aggggattaa cgagacgttg tttatggcgt
    15901 tgattacgct ggagtctcag gaaaaccaca gtattcagcc ttctgaatta agttgtgctc
    15961 ttggatcatc ccgtaccaac gcgacgcgta ttgccgatga actggaaaaa cgcggttgga
    16021 tcgaacgtcg tgaaagcgat aacgatcgcc gctgcctgca tctgcaatta acggaaaaag
    16081 gtcacgagtt tttgcgcgag gttttaccac cgcagcataa ctgcctgcat caactctggt
    16141 ccgcgctcag cacaacagaa aaagatcagc tcgagcaaat cacccgcaaa ttgctctccc
    16201 gtctcgacca gatggaacaa gacggtgtgg ttctcgaagc gatgagctaa cgcgtcatct
    16261 cgctcaaaaa tccagattta taaaagaaaa aatgaccggc cagcatcgca acatgctggc
    16321 ctttttggca agcaggtcgg ctcagccgat gagttaagaa gatcgtggag aacaatatga
    16381 gcgcaaatgc ggagactcaa accccgcagc aaccggtaaa gaagagcggc aaacgtaagc
    16441 gtctgctcct ccttctcacc ttgctcttta taattattgc cgtagcgata gggatttatt
    16501 ggtttttggt actgcgtcat ttcgaagaaa ccgatgacgc atacgtggca gggaatcaga
    16561 ttcaaattat gtctcaggtg tctggcagcg tgacgaaagt ctgggccgat aacaccgatt
    16621 ttgtaaaaga aggcgatgtt ctggtcactc tcgacccgac agatgctcgc caggcgtttg
    16681 aaaaagccaa aactgcactg gcttccagcg ttcgccaaac ccaccagctg atgattaaca
    16741 gcaaacagtt gcaggcgaat attgaggtgc aaaaaatcgc cctcgcgaaa gcacaaagcg
    16801 actacaaccg ccgtgtgccg ctgggcaatg ccaacctgat tggtcgcgaa gagctgcaac
    16861 acgcccgcga cgccgtcacc agtgcccagg cgcaactgga cgtcgctatt caacaataca
    16921 atgccaatca ggcgatgatt ctggggacta aactggaaga tcagcctgcc gtgcaacagg
    16981 ctgccaccga agtacgtaac gcctagctgg cgctggagcg tacccgtatt gtcagtccga
    17041 tgaccggtta tgtttcccgt cgtgccgtgc aaccgggtgc gcaaattagc ccaacgacgc
    17101 cgctgatggc ggtcgttcca gccaccaata tgtgggtgga tgccaacttt aaagagacgc
    17161 agattgccaa tatgcgcatc ggtcagccgg tcaccatcac caccgatatt tacggcgatg
    17221 atgtgaaata caccggtaaa gtggttggtc tggatatggg cacaggtagc gcgttctcac
    17281 tgcttccggc gcaaaatgcg accggtaact ggatcaaagt cgttcagcgt ctacctgtgc
    17341 gtatcgaact ggaccagaaa cagctggagc aatacccgct gcgcattggt ttgtccacgc
    17401 tggtgagcgt caataccact aaccgtgacg gtcaggtact ggcaaataaa gtacgttcaa
    17461 caccggtcgc ggtaagtacc gcgcgtgaaa tcagcctagc acctgtcaat aaactgatcg
    17521 acgatatcgt aaaagctaac gctggctaat ccagaggtgc gtgtgatgca acagcaaaaa
    17581 ccgctggaag gcgcgcaact ggtcattatg acgattgcgc tgtcactggc gacattcatg
    17641 caggtgctgg actccaccat tgctaacgtg gcgatcccca ctatcgccgg gaatctgggc
    17701 tcatcgctca gccaaggaac gtgggtaatc acttctttcg gggtggcgaa tgccatctcg
    17761 atcccgctta ccggctggct ggcaaagcgc gtcggggaag tgaaactgtt cctttggtcc
    17821 accatcgcct ttgctattgc gtcgtgggcg tgtggtgtct ccagcagcct gaatatgctg
    17881 atcttcttcc gcgtgattca ggggattgtc gccgggccgt tgatcccgct ttcgcaaagt
    17941 ctattgctga ataactaccc gccagccaaa cgctcgatcg cgctggcgtt gtggtcgatg
    18001 acggtgattg tcgcgccaat ttgcggcccg atcctcggcg gttatatcag cgataattac
    18061 cactggggct ggatattctt catcaacgtg ccgattggcg tggcggtggt gttgatgaca
    18121 ctgcaaactc tgcgcggacg tgaaacccgc accgaacggc ggcggattga tgccgtgggg
    18181 ctggcactgc tggttattgg tatcggcagc ctgcagatta tgctcgaccg cggtaaagag
    18241 ctggactgtt tttcatcaca ggaaattatc attcttaccg tggtggcggt ggtggctatc
    18301 tgcttcctga ttgtctggga gctgaccgac gataacccga tagtcgatct gtcgttgttt
    18361 aagtcgcgca acttcaccat cggctgcttg tgtatcagcc tcgcgtatat gctctacttc
    18421 ggcgctattg ttctgctgcc gcagttgttg caggaggtct acggttacac ggcgacctgg
    18481 gcaggtttgg cctctgcgcc ggtagggatt attccggtga ttctgtcgcc gattatcggc
    18541 cgcttcgcgc ataaactgga tatgcggcgg ctggtaacct tcagctttat tatgtatgcc
    18601 gtctgcttct actggcgtgc ctataccttt gaaccaggta tggattttgg cgcgtcggcc
    18661 tggccgcagt ttatccaggg gtttgcggtg gcctgcttct ttatgccgct gaccaccatt
    18721 acgctgtctg gtttgccacc ggaacgactg gcggcggcat cgagcctctc taactttacg
    18781 cgaacgctgg cggggtctat cggcacgtcg ataaccacga ccatgtggac caaccgcgag
    18841 tcgatgcacc atgcgcagtt gactgagtcg gtaaacccgt tcaacccgaa tgcccaggcg
    18901 atgtacagtc aactggaagg gcttgggatg acgcaacagc aggcatcagg ctggattgcc
    18961 cagcagatca ccaatcaggg gctgattatt tccgccaatg agatcttctg gatgtcagcc
    19021 gggatattcc tcgtactgct ggggctggtg tggtttgcta aaccgccatt tggcgcaggt
    19081 ggcggcggag gcggtgcgca ctaagtacaa ctaagccagt tcatttgaac tggctttttt
    19141 caattaattg tgaagatagt ttactgacta gatgtgcagt tcctgcaact tctctttcgg
    19201 cagtgccagt tcttcgttgc tgttgatgcg tacgtcacgt tccagaatgc tacgcgcaat
    19261 atcctgcgct tcctgcaacg agtgcatctg gtaagtgcca cactggtaga cgttcagttc
    19321 cgggatctga ttctgatcct gcactttcag cacgtcttcc attgccgctt tccaggcatc
    19381 agcaacacgc tgctcatctg gcgtaccaat cagactcata taaaaaccgg tgcggcagcc
    19441 cattggcgag atatcgataa tctctacacc attaccgtta agatggttac gcataaaacc
    19501 agcaaacagg tgctccaggg tatggatccc tctttctggc atcacttctt tgttcggcac
    19561 gcagaagcgc agatcgaaca cggtgattgc gtcgccatgc ggggtgttca ttgttttcgc
    19621 cacccgaact gcaggcgctt ccatccgggt atgatcgact gtgaagctat ctaacaacgg
    19681 catttagcca cctccggtaa tttttttaaa aattttctga actctttctt cccaggcgag
    19741 tctgagtata tgaaagacgc gcatttgtta tcatcatccc tgaattcaga gatgaaattt
    19801 tggccactca cgagtggcct ttttcttttc tgtcaggcgt gtttttccag ccacaccgca
    19861 aacggttcgg tatcagcggt ttccatttcc tgctgacggc gttcagatgc ctcgcgctcg
    19921 gctacaaaat cctcttcgcg cagaatttcc agcggctctt cacgcagcag attacggtag
    19981 gcttctgcaa acgctttgcc tgttccgcca ataccagtat caatcataga ccttaagata
    20041 cgggcagaga aagtcagatc gggattatcg aagcaggcaa ccagttcatc acacactttc
    20101 tgatacgctt cgccgccgtt aatgctatcc agcgtttgcg cgacgcgttt cagatcgtgg
    20161 aacagatctt tacccacctg cagtaacggg aactgcgcgg tttcgcagcc gatacccagc
    20221 gtcaggcccg gtttgcgacc ttcaaggatc acccggttcc agttaacgcg cgtacaggca
    20281 agttcgctac tgctcatttc cggtgcatca gccagcgcac accagaccat aaacaggtcg
    20341 aggaatcgca cctgctgttc atctacacca atcggcgaga acgggttgat gtccagcgaa
    20401 cgcacttcaa tatattcaat gccgccacgt aacagcgcat cagaaggtga ctcgccgctg
    20461 cgggtaacgc gttttggacg aatcggcgca tacagttcgt tttcaatctg cagcacgttg
    20521 ctattgattt gcagcctctt accgtctttc tcaataccaa tcttcgcgta ctcttccgat
    20581 ggcgttttga ttgcctgttt aaggcccgct acgtactcgt aaagatcgtt gaaggtaata
    20641 ccaagattgc tttgcgattt attggtatag ccgagatcgc tcaaacgaag agaggtcgca
    20701 tacggcaggt aatacatacc gcactcggtt ttctcaaacg gcagcgacgt tggttttcct
    20761 tgcaggaaag aagaacaaat cgccggagat gcaccaaaca gataaggaat gacccaaccg
    20821 aaacgatagt aattgcggat aacgcggaaa tagcccgcag aaattttctc tttggcatca
    20881 gcgcccgaga tatcaccgca cttcgcttgc cagaatgcca ttggcaaaga gaaattgtag
    20941 tgcacgccgg aaatggtttg catcagcgcg ccgtagcgat ttttcagccc ttcacgatac
    21001 agcgttttaa agcgtccggt gttagaagtg ccgtactgtg ccagttcgat gtcctgacct
    21061 tctgcgatgt agcatggcat acttaacggc cacatccgct catcgcccat attgcgcgcc
    21121 gtataacgat gcagatcgcg cataaaggtc agcatatgtt caatatcacc atccactggt
    21181 gtaatgaatt ccagcaatgc ttccgcaaaa tcggtagtaa tccatttgtg cgtcagtgcg
    21241 gaacctaatg cttcaggatg acctgttgtt gccagtgtgc catcagcatt aacacgcaaa
    21301 gtttcgcgct ccagcccacg ctgtatcccc tttaacgcct gaggatgttt ttccagccag
    21361 gccagcgcct gtgatacgtc cgggatcaaa ttgacctccc gcctgtcaaa atcgttttaa
    21421 ttagcataac tgtaatggtg accatatgtg caggcctaca attagtgcca ccacatcatg
    21481 ccctgaacgg tcgctgctgc aactgcaaca tagcgtaacg ctttaccaag gcacaaaaaa
    21541 aagattactg gcccccagga gatgcgcatc catcccgcta acagacacag taaatcgcca
    21601 accacgggca tccagcttaa taatagcgtg actgcaccat agcgtttcag ccagccggta
    21661 gctttctctt gccagcgcga tgttttacgc aatggaaaga aacgcccaag gataacgtta
    21721 gttaaccctc caaggctatt acccattgtt gctgttaaaa ctaaaaccta gggatgactg
    21781 atcccggaaa gcaacattgc caccagcacg acttcggagt tgccgggtaa tagtgtagcg
    21841 ctgagaaaac tactggcaaa caacgagaaa agcgataacg cttcactcac agcaagcgaa
    21901 catccacagc gtccatgcct gctgcacggg ccgcctgaat accgaaatcg gcatcttcaa
    21961 agaccacaca ctgcgtcggt tgcacgccca tacgctgcgc gcacaacaaa aatgtgtctg
    22021 gcgcgggttt atggtgtttg acgtgatcag cagcgacgac ggcgtcaaaa taacggcgta
    22081 atcccaggtg cgccagcaat gcctctgcga tggcgctttc actccccgtt cctacagcca
    22141 ttgggcgacg accatgccaa cttttcacca cttcaacaag aggaagcggc tcgacactat
    22201 ccagcagcat acttcttact gcttctgttt tttcacgcgc taacgcatgc gggtcgagat
    22261 cggcctgatt cagctcaata attgcctgag caatacgcca ggtgggcgat ccattaagcg
    22321 caatcatcgc ctgaatatcg tactgaagac cgtagtgccc taatacttcg cgccacgctt
    22381 tacggtgcgt aggctccgta tccaggattg tgccatccat atcaaaaatt aaacctgcat
    22441 aacgctcgta catggtcttc tcgcaagtcg aaaaatatga cgctacttta gcgtaattgc
    22501 ttgattttgt cgctgatggc gaaggagaag aaaattcagg aaatggataa agtagttatt
    22561 tagaaatgag atatttttga aggaaatttt ttggagaa
//
LOCUS       DAJTXY010000060        22314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTXY010000060 DAJTXY010000000
VERSION     DAJTXY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.060"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="OO566_002370"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="OO566_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6605626.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            831..971
                     /locus_tag="OO566_002371"
     CDS             831..971
                     /locus_tag="OO566_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605627.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            985..1857
                     /locus_tag="OO566_002372"
     CDS             985..1857
                     /locus_tag="OO566_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR6605628.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKNETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1917..3215)
                     /gene="eno"
                     /locus_tag="OO566_002373"
     CDS             complement(1917..3215)
                     /gene="eno"
                     /locus_tag="OO566_002373"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6605629.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="OO566_002374"
     CDS             complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="OO566_002374"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6605630.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="OO566_002375"
     CDS             complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="OO566_002375"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6605631.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6055..8289)
                     /gene="relA"
                     /locus_tag="OO566_002376"
     CDS             complement(6055..8289)
                     /gene="relA"
                     /locus_tag="OO566_002376"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HCR6605632.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="OO566_002377"
     CDS             complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="OO566_002377"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6605633.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9695..12451
                     /gene="barA"
                     /locus_tag="OO566_002378"
     CDS             9695..12451
                     /gene="barA"
                     /locus_tag="OO566_002378"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6605634.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="OO566_002379"
     CDS             complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="OO566_002379"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6605635.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14044..15384)
                     /locus_tag="OO566_002380"
     CDS             complement(14044..15384)
                     /locus_tag="OO566_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6605636.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15386..16738)
                     /gene="gudP"
                     /locus_tag="OO566_002381"
     CDS             complement(15386..16738)
                     /gene="gudP"
                     /locus_tag="OO566_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6605637.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17172..17621)
                     /locus_tag="OO566_002382"
     CDS             complement(17172..17621)
                     /locus_tag="OO566_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6605638.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17639..18421)
                     /gene="truC"
                     /locus_tag="OO566_002383"
     CDS             complement(17639..18421)
                     /gene="truC"
                     /locus_tag="OO566_002383"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6605639.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTVHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18421..18750)
                     /locus_tag="OO566_002384"
     CDS             complement(18421..18750)
                     /locus_tag="OO566_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6605640.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19372..19917)
                     /gene="syd"
                     /locus_tag="OO566_002385"
     CDS             complement(19372..19917)
                     /gene="syd"
                     /locus_tag="OO566_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6605641.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRMHLASNLAEFLNQLKPLL"
     gene            19985..20833
                     /gene="queF"
                     /locus_tag="OO566_002386"
     CDS             19985..20833
                     /gene="queF"
                     /locus_tag="OO566_002386"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6605642.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20945..22309
                     /gene="ppnN"
                     /locus_tag="OO566_002387"
                     /gene_synonym="ygdH"
     CDS             20945..22309
                     /gene="ppnN"
                     /locus_tag="OO566_002387"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6605643.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5512 a         5912 c         5292 g         5598 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag
      841 aaaataaaga aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt
      901 ctgtattagt ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta
      961 tccagattta atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt
     1021 tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt
     1081 acgagtagct taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc
     1141 aataataccc aagtgcaaat tgctgtatta attgtttcca caaccaaaaa tgagacaatt
     1201 gaacaatatg ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     1261 gggatactga tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     1321 ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc
     1381 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     1441 caactcactt cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc
     1501 actgatcatt attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     1561 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca
     1621 atccatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc
     1681 ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga
     1741 gcatctggta gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc
     1801 ggatttagcg gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc
     1861 caccataaaa aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat
     1921 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     1981 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     2041 atctggcctg cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca
     2101 gaacggtgag agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga
     2161 gtttcggtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     2221 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     2281 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     2341 ccgtcttcga tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat
     2401 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag
     2461 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     2521 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     2581 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccagg
     2641 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     2701 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     2761 ttcatcatcg gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc
     2821 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     2881 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     2941 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     3001 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     3061 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     3121 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     3181 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     3241 aactgaaact ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt
     3301 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     3361 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     3421 gggtgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg
     3481 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     3541 tgacgctcaa caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac
     3601 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     3661 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     3721 tctggcacaa attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     3781 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     3841 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     3901 attgcgtcca gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     3961 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact
     4021 gatttataag catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt
     4081 accggattcg cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag
     4141 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     4201 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     4261 aacaatgcaa tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg
     4321 atgtcaggct ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt
     4381 ttgacttcac cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc
     4441 tcacggccaa tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata
     4501 tcacctactg taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc
     4561 acgcgctctt tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag
     4621 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     4681 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca
     4741 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     4801 acgttgatgt aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     4861 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     4921 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     4981 ccaagacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     5041 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     5101 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt
     5161 aattccctta gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt
     5221 cgaggtcaac acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa
     5281 aacgacgctc gaatttttcg ttcacttttt gcaatgcgat ttctgctttc gtccctaaat
     5341 ggcgagccag attaaccgtg gcaaacagca ggtcccccat ttcctcctcc agtttagcct
     5401 ggtcgacaac agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat
     5461 caaccaccgg gccaagcgtc gtccagtcga agccaacgtt ggcgcaacgt ttctggattt
     5521 tttgcgcccg cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg
     5581 ctttctgcgc gcgctcttcg gttttgattt gctcccaacg ggcaagcact tcactactgt
     5641 tttcggcaga actatcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag
     5701 cagcgcaaat atcattaaag tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca
     5761 ccacctggaa tagcagatcg cccagttcac cgcgaagatc gtcaaaatct tcacgggcga
     5821 tggcgtccag cacttcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct
     5881 gctctttatc ccacgggcag ccgttttccg gatcgcgcag gcgctgcata atagtgagca
     5941 aacggtcgat ttgattcatt gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc
     6001 ttcgagtaaa agatatcgct ggatacggct gacgccatat ccagctatgt aagactaact
     6061 cccgtacaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg
     6121 ccccagcact tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt
     6181 ggtgtcgcta cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt
     6241 gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc
     6301 ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag
     6361 ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa
     6421 gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt
     6481 accaacacct tcaaccacca cacgaccgtt atctttactg cggttttgcg gtgtgtagct
     6541 tttttgctga agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa
     6601 ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc
     6661 cagcaactcg tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt
     6721 caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt
     6781 tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt
     6841 gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat
     6901 aatttcaatc tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat
     6961 ttttgccccg atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg
     7021 cgttgatccc gcaggcaaat caacgacatc acctttcggc gtaaagacat acacccggtc
     7081 gtcaaacacc tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg
     7141 ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc
     7201 agctgccgcg ccctctttat atttccagtg cgcagcaaca cccaactccg catcttcatg
     7261 catctgtttg gtgcggattt ggatctcaac agtttttcca cccggcccca gaaccacagt
     7321 atgaatagac tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg
     7381 caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc
     7441 ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg
     7501 cattttgcgc cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc
     7561 ttcagctttc atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc
     7621 gagacggcgt tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg
     7681 gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg
     7741 cgcgtagata ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt
     7801 tacttcgcgc agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc
     7861 gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt
     7921 gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac
     7981 aaccgactta ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag
     8041 agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc
     8101 caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg
     8161 cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag
     8221 acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac
     8281 cgcaaccatc gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat
     8341 ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc
     8401 atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc
     8461 gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc
     8521 ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc
     8581 gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt
     8641 aagccattaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca
     8701 ccgaccacac tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac
     8761 agatccagta cgcgatcttc aggttgcaca tccagccatt ccaacgcacg cgctaccatt
     8821 ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac
     8881 ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg
     8941 gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc
     9001 gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt
     9061 tcaacatgac caagatggcg catagcttgt aagctgccca gacatgccct gactttgggc
     9121 agcaatgctt caagttgggg cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg
     9181 gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt
     9241 aaacgcgcgc ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt
     9301 ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt
     9361 tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg
     9421 ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca
     9481 gcaacttccg cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt
     9541 cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc
     9601 gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa
     9661 cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg
     9721 catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt
     9781 cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg ctagcattat
     9841 tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg
     9901 tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta
     9961 tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca
    10021 gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat
    10081 gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga
    10141 tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt
    10201 tcgcttgcag caatataaag agatctttat ttcctgcgtg atgatgctgt tttgtatcgg
    10261 tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat
    10321 ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat
    10381 gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc
    10441 ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct
    10501 ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc
    10561 ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa
    10621 tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga
    10681 tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct
    10741 cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat tcccactacg
    10801 cagcacgctg gatgaagtcg ttactctgct ggcacattct tctcacgata aagggttgga
    10861 actgacgctt aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt
    10921 acagcaaatc atcactaacc tagtggggaa tgcaattaaa ttcaccgaga atggcaacat
    10981 tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat
    11041 tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg
    11101 acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac
    11161 acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg
    11221 ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc
    11281 atccatccag tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc
    11341 gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt
    11401 ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg
    11461 cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct
    11521 gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat
    11581 cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt
    11641 ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac
    11701 agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga
    11761 tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcagttgaac gggcgaaaca
    11821 gatgccgttc gatttgatct taatggatat tcaaatgccg gacatggatg gcattcgggc
    11881 ctgcgaactc atccaccaac tcccgcatca gcgacaaacg ccggttatcg cggtaacggc
    11941 gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc
    12001 gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg
    12061 tatttcgtct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac
    12121 cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat
    12181 gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt
    12241 tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta
    12301 tagcggcgta ccacgtatga agaatctctg ccagctgatc gaacaacagc tacgtagcgg
    12361 tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt
    12421 cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt
    12481 tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt
    12541 aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc
    12601 tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaaaccg gctccagata
    12661 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    12721 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    12781 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    12841 tttctggcac ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    12901 taccttcctg ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    12961 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg
    13021 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcata gtccagaaat
    13081 gcggatccgc cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc
    13141 agtcggtggc gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca
    13201 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    13261 aacctttcag gtatttacca attttaattg cttcgttcag cgaccaggca ccgttaggat
    13321 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcttctt
    13381 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg
    13441 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    13501 accagtcgca tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac
    13561 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    13621 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    13681 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccactagcat
    13741 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    13801 tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    13861 tttcccctac gccagtgtga ccggaattat ctttgataat cacaatatta cgcgtgaaga
    13921 acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga
    13981 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    14041 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    14101 ctgcatcgga cctgcgtcgt tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc
    14161 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    14221 ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat
    14281 ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaatat
    14341 cgccagagag atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc
    14401 gcaaagctgc gccacgcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    14461 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    14521 gttagtcgcg acgggcaagc cggtcgcccg tcgaaactcc gccatcactt cacgtccgga
    14581 gaagccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    14641 gcataaagaa atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc
    14701 atccgggaag cttttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac
    14761 cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg
    14821 cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc
    14881 cggcgtattt tccagataag gaagatcggt tttggtccga tcacctatat aaaacagata
    14941 accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac
    15001 attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac
    15061 gcgcaattca aaagtccagg cacctttgcc gaaggtctca aaatcggctg cctgatttcc
    15121 tttatgtacc tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg
    15181 aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt
    15241 atgcccggcg ttatcggtga gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc
    15301 accaatattg agcaacatgc tgtcatgtcc ggcgaccgga atgactttca tatcagtaat
    15361 aacaggactg gattgtgtcg ccatcttatt gccccgcgac aggtttcagc tcgatacgct
    15421 tgatatcgcc caccagcacc aggtagctca gtaccgcgat tagggcatga acaccaacat
    15481 aaatcagcgc cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    15541 tgacgatgcc agaaatgtta ccgaacatgt tgaactggcc accggaaaga ccgctgatct
    15601 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    15661 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    15721 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc
    15781 ccgtgcggcg catcagccag tcggaaataa ttccacccag cacaccgccg ataaaaccgc
    15841 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca
    15901 ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac
    15961 cgatataaac gccgatcatc atccgtgacc cgagcagctg tttgatctgc ccccacttca
    16021 cgctgaacgg cactttaact ttggtgcttt gctgatccat attgatcagc gcgccacccg
    16081 cggcgatata ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    16141 ctttcaacca gataaagctg atgacaatgc ccagaccgcc cataaagaag aagacgtgtg
    16201 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    16261 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    16321 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    16381 gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    16441 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    16501 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    16561 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag
    16621 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    16681 agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg
    16741 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    16801 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    16861 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    16921 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    16981 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga
    17041 gtatgaaatg gatcgcttga ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag
    17101 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    17161 tcgctgacat atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt
    17221 tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc
    17281 tgttcctgca acaaggcatc gaactgtttg ccgccattgc aaaaatttac ataactgctg
    17341 tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt
    17401 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    17461 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    17521 gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa
    17581 tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    17641 aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag
    17701 gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    17761 cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta
    17821 gcattaaccg ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatctccat
    17881 gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt
    17941 tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    18001 tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc
    18061 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    18121 ccaacaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    18181 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    18241 acacaccaga agtgggtcgg tccagacgat gaacagtaaa aacatgctgt cctatctggt
    18301 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagcta cggtgaacca
    18361 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    18421 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc aactcagcca gaataagcgc
    18481 gcgttgcgga tggtccgtcg ctagcgccat ttcataatag ggtgcaacag caaaagcgcc
    18541 cggtaacggc tggttgttat ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa
    18601 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg
    18661 ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    18721 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    18781 aaggatagca ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc
    18841 agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    18901 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    18961 atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    19021 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    19081 ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc
    19141 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    19201 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt
    19261 ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    19321 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttgg attacagaag
    19381 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcatcc gtttacgtgt
    19441 tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    19501 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    19561 atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa
    19621 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    19681 ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    19741 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    19801 cggtgaagga acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    19861 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    19921 tttctctctt cacttaaacc agatacactt gtcgcttagt ttatctggtt tatgacggtg
    19981 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    20041 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    20101 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    20161 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga
    20221 acttgattac accagcgtaa atctgattga gtcgaagagt tttaagctct atctcaacag
    20281 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    20341 aagcacttgc gctcagggta agattagcgt ggcgttatat cgtcttgatg aactggaagg
    20401 ccagccgata ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa
    20461 ctatgaattc actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga
    20521 gacgctcgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg
    20581 ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    20641 ggtctcattc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    20701 cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg
    20761 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    20821 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    20881 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    20941 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    21001 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    21061 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    21121 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc
    21181 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    21241 ggttctgcga gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa
    21301 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    21361 tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat
    21421 taacgaaaac gagtatttgt atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct
    21481 gaatatctgc accggctgtg gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt
    21541 cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    21601 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    21661 aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggcggtgt
    21721 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    21781 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    21841 ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat
    21901 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    21961 tcgccgtgat acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt
    22021 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    22081 acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    22141 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga
    22201 taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat
    22261 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctcc
//
LOCUS       DAJTXY010000061        21999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTXY010000061 DAJTXY010000000
VERSION     DAJTXY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.061"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..156
                     /locus_tag="OO566_002388"
     CDS             <1..156
                     /locus_tag="OO566_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6605644.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
     gene            226..1032
                     /locus_tag="OO566_002389"
     CDS             226..1032
                     /locus_tag="OO566_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6605645.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1042..1449
                     /locus_tag="OO566_002390"
     CDS             1042..1449
                     /locus_tag="OO566_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6605646.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1449..1943
                     /locus_tag="OO566_002391"
     CDS             1449..1943
                     /locus_tag="OO566_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6605647.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2009..2806
                     /locus_tag="OO566_002392"
     CDS             2009..2806
                     /locus_tag="OO566_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6605648.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2817..3605
                     /locus_tag="OO566_002393"
     CDS             2817..3605
                     /locus_tag="OO566_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6605649.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3657..4886
                     /gene="sorE"
                     /locus_tag="OO566_002394"
     CDS             3657..4886
                     /gene="sorE"
                     /locus_tag="OO566_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6605650.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMELSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5268..5957
                     /gene="pepE"
                     /locus_tag="OO566_002395"
     CDS             5268..5957
                     /gene="pepE"
                     /locus_tag="OO566_002395"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6605651.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6048..7679)
                     /locus_tag="OO566_002396"
     CDS             complement(6048..7679)
                     /locus_tag="OO566_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6605652.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7899..11582)
                     /gene="metH"
                     /locus_tag="OO566_002397"
     CDS             complement(7899..11582)
                     /gene="metH"
                     /locus_tag="OO566_002397"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6605653.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISSDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTMPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11782..12606
                     /gene="iclR"
                     /locus_tag="OO566_002398"
     CDS             11782..12606
                     /gene="iclR"
                     /locus_tag="OO566_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6605654.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12923..15107
                     /gene="espL4"
                     /locus_tag="OO566_002399"
                     /pseudo
     CDS             12923..15107
                     /gene="espL4"
                     /locus_tag="OO566_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(15076..16812)
                     /gene="aceK"
                     /locus_tag="OO566_002400"
     CDS             complement(15076..16812)
                     /gene="aceK"
                     /locus_tag="OO566_002400"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR6605655.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16996..18300)
                     /gene="aceA"
                     /locus_tag="OO566_002401"
     CDS             complement(16996..18300)
                     /gene="aceA"
                     /locus_tag="OO566_002401"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR6605656.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGESMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18330..19931)
                     /gene="aceB"
                     /locus_tag="OO566_002402"
     CDS             complement(18330..19931)
                     /gene="aceB"
                     /locus_tag="OO566_002402"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR6605657.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20200..21129)
                     /gene="metA"
                     /locus_tag="OO566_002403"
     CDS             complement(20200..21129)
                     /gene="metA"
                     /locus_tag="OO566_002403"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6605658.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21286..21726
                     /locus_tag="OO566_002404"
                     /pseudo
     CDS             21286..21726
                     /locus_tag="OO566_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
     gene            complement(21843..21958)
                     /gene="rrf"
                     /locus_tag="OO566_002405"
     rRNA            complement(21843..21958)
                     /gene="rrf"
                     /locus_tag="OO566_002405"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5409 a         5641 c         5569 g         5380 t
ORIGIN      
        1 ctctctatcg aaatgaataa attaaagcag gcgcgatatt ccattggcat tgccatgagt
       61 gaagaaaaat acagcggaat tattggtgca ctgcgtggaa aatatattaa ttgtctggta
      121 acgaatagca gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa
      181 taaaaattac ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta
      241 aatttgcagg ataaaatcat tattgtcacc ggcggcgcat ccggtattgg tctggcgatt
      301 gtagaggaat tattagcaca aggcgcgaat gtacagatgg tcgatattca cggtggcgat
      361 ggtcaatatg aaggtcataa aggctatcag ttttggccga ccgatatttc tagcacaaaa
      421 gaggtaaatc atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc
      481 aataacgccg gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag
      541 tatgaactca acgaagctgc attcgaaaaa atggtcaata tcaaccagaa aggtgttttt
      601 ctgatgtcgc aggcggtggc gcgacagatg gtcaaacaac atgatggcgt gattgtgaat
      661 gtttcctcag aaagtgggct ggaaggctca gaaggacaaa gctgttacgc cgctaccaaa
      721 gccgcgctca atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt
      781 gtggtcggta tcgcgcccgg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa
      841 gaggcgctgg cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa
      901 aacgccattc ctattgggcg cgccggaaga ttagcagaag tagctgattt tgtttgttat
      961 ctgttgtctg aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa
     1021 acgcgcggct aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct
     1081 tgcgccatgc tggaatcggt tcagatggtt tacggcaacg ccaatgttga agccgttgaa
     1141 tttgtgcccg gtgaaaacgc cggagacatt gcggtaaagc tggaaaagtt agcaagcatt
     1201 cacaatcaag atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg
     1261 gcagcaatgc ttgcgatgcg caatcctcgt ttgcgggtaa tcagcggcct ttctctgccg
     1321 ctggcgctcg aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac
     1381 ctgacgcaaa tcgcaaagca aacctgcgtt gtctggaaac aactggcaac gacagaggag
     1441 gatttctgat gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca
     1501 ctaccgtctg gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt
     1561 ataacgatga agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta
     1621 acgtggtcaa tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat caggacgaaa
     1681 ccgtttttta tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa
     1741 aaataggcac cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca
     1801 aagcggtttc attagatgat gatgatatta acgcatttca tgagttaaat aatcttggtg
     1861 tcatactgga tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa
     1921 acgaacagtt aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg
     1981 gatgacgaca cttaaaaggt gacaaatcat ggaaataagt accctacaaa tcattgctat
     2041 atttcttttt tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg
     2101 cccgttaatt gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat
     2161 actcggcggt acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc
     2221 tccggattct gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag
     2281 catcgccacc ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccagg tgctgacggt
     2341 gtttgcccgt accatcaccg tagtgttcca gcacgcggcg gataaagcag cagaagaggc
     2401 gcggtttcgc actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt
     2461 cgccattccg gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt
     2521 aaacgccatt cccgaatttg tgacccgagg actacagatt gctggcggtt ttatcgtggt
     2581 ggtcggttac gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt
     2641 cctcggtttc ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg
     2701 cgtgatcatg gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca
     2761 actccaggcc accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg
     2821 aacagagaaa aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac
     2881 aggcgtcctt taactttgaa cgtattcacg ggctgggctt ttgctacgac atgatccccg
     2941 ccatcaagcg actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg
     3001 tgttcttcaa taccacgcca gccgtatgtg gcccggtcat cggcgcggaa attgatgacg
     3061 gtaccatcaa cggcatcaaa gtcggtctga tgggaccatt ggcaggagtt ggcgatccac
     3121 tggtctgggg aacgctgcgc ccgattaccg ccgcgctcgg cgcatctctg gcactttcgg
     3181 gcaacattct cggcccgctg ctgttcttct ttattttcaa cgcggtgcgt ctggcgatga
     3241 agtggtatgg cctacagctc ggctttcgca aaggggtgaa tatcgtcagc gatatgggcg
     3301 ggaatgtgct gcaaaaactc accgaaggcg cgtcgattct cggactgttt gtgatgggcg
     3361 tgctggtgac caaatggacg tcaatcaacg taccgttggt ggtttcacaa acgcatgccg
     3421 ccgatggctc caccgtcacc atgaccgtgc agaacattct cgaccaactt tgccctggtt
     3481 tgctggcgct cggtctgacg ctactaatgg ttcgtctgct caacaaaaaa attaacccgg
     3541 tatggctgat tttcgccctg tttggcttag ggattatcgg caatgctctg ggcttcctgt
     3601 cctgattctt cgccccggca cgactgccga ggccatcgct caacatgagg tggtttatga
     3661 aaacaacagc tctgcgtctt tatggtaaac gtgatttacg cctggaaacc tttgaccttc
     3721 ctgaaatgca ggaggatgaa atcctcgcaa cggtggtcac tgacagcctg tgcctctctt
     3781 cctggaaaga ggccaatctg ggtgaaaacc ataaaaaagt acccgacgat gtggcgacca
     3841 acccaatcat catcggccac gaattttgcg gcgatattct ggccgtgggt aaaaagtggc
     3901 agcacaaatt ccagccgggt cagcgttatg tgattcaggc caacctgcaa ctccccgacc
     3961 gcccggattg ccccggctac tccttcccgt gggtaggcgg cgaagcgacg catgtggtta
     4021 ttcccaacga ggtcatggaa caagattgcc tgctggcata cgacggcgaa acctattttg
     4081 aaggctcact ggttgaaccg ctttcctgcg tgattggcgc gttcaacgcc aactatcatc
     4141 ttcaggaagg tagttataac cacacgatgg ggattcgccc gcaagggcgc acgctgatcc
     4201 tcggcggcac cggaccaatg ggactgttgg cgattgatta tgcgctgcat ggaccagtta
     4261 acccgtcgct gctcgtcatt accgataccg acaacgacaa attgagttat gcgcgcaagc
     4321 actatccatc agaaccgcaa acactgattc attatctcaa tgccaccgat gcagcatttg
     4381 atacgttaat ggagctgagt ggcggtcacg gcttcgatga tattttcgtc tttgtgccta
     4441 atgaaggact ggtgactctc gcctcttcct tgctggcgac agatggttgc ctgaatttct
     4501 tcgccggacc gcaggataaa catttcagcg cgccaattaa tttctacgat gtgcattatg
     4561 catttaccca ctacgtgggc acgtcaggcg gcaataccga cgacatgcgc gcggcggtca
     4621 aactgatcga agagaaaaaa gtgcaggccg caaaagtggt aacacatatt cttgggctga
     4681 atgccgcggg cgaaaccacg cttgaattgc ctgccgtcgg cggcggcaaa aagctggtgt
     4741 ataccgggaa atacctgccg ctgacgtcac tcacgcagat tcaggatcaa gcactggcgg
     4801 cgattctggc gcgtcatcag gggatctggt ccggtgaggc ggagcaatac ctgctcgccc
     4861 atgcagaggc aatttcccat gattaatcgc gctacttaac tctgcatgtc gctggctggt
     4921 cgccccagca acttcgcgac cggatgtgcg gctgattgct gaagcagcac aatttgcacg
     4981 agaaggataa aaaatatcag cgccttaagg ggcgctcaaa ttggtggcaa agtacacatt
     5041 gtttatgccg gatgcggaat gaagccttat tcggtctaca taattttgaa aatccaatat
     5101 attgcagtta tctggtaggc caggtaagcg tagcgcatca ggcaatgctg tgtttgtcat
     5161 cagtctcagc gcccggtgca atacgtgaca tcgtgagcca tgtcagtaag tggttaaatt
     5221 cgcagtggta gcatgccttc cgtgatttct tactaaccgg agaagtaatg gaactgcttt
     5281 tattgagtaa ctcgacgctg ccgggtaaag cctggctgga acatgcactg ccgctaattg
     5341 ctgaacagtt gcagggtcgc cgctcagcgg tgtttatccc tttcgctggc gtaacgcaga
     5401 cctgggatga ttacacagag aaaacggctg cggttctcgc tccgctgggt gtttctgtca
     5461 ccggtattca tagcgttgtc gatcccgttg ccgcgattga aaatgcggaa attgtgattg
     5521 tcggcggcgg taatactttc cagttgctga aacagtgccg cgagcgcggg ctgctggctc
     5581 caattactga cgtggttaaa cgtggcgcgc tgtatattgg ctggagcgca ggcgctaacc
     5641 ttgcttgccc aaccattcgt accaccaacg atatgccgat tgtcgatccg caaggtttcg
     5701 atgcgctaaa tctgttcccg ttgcaaatca acccgcactt caccaacgcg ctgccggaag
     5761 gccataaagg tgaaacccgt gagcagcgta ttcgcgaact gctggtcgtc gcgccagaac
     5821 tgacgattat tggtctgccg gaaggtaact ggatcacagt gagtaaaggt cacgctacgc
     5881 tgggtggccc gaacaccact tatgtgttta aggctggtga agaagcggtt ccgctggaag
     5941 ctggtcaccg tttttaagtg aattgccgga tgcatgatcc ggcatcttgt tcatagtgcc
     6001 tgatgcgacg cttgacgcgt cttatcaggc ctacaggatt cacaatatta atactcgtcc
     6061 cgtccttcat cttcatccgg ctgttccagc acactgtaag ccaccgaaca aaacagcgag
     6121 ttcaggcgct gcatatctcc cagtaagcct aaatgtagcg aactggtttc aatgctctgc
     6181 acgttttgct gatgcaggcg atcgacgtgg gcgtgggaat agcggcgatt aagaatgcga
     6241 aaacgatgtt tgctgcgacg caaacgacga gcgctggtga catcgccaga gaagaacacc
     6301 gacattgcca gctttaaatt actgagcaat tgctcataga gcgcatccag ttccttcaac
     6361 ccatcaagcg aaaatgcccg ccgtgctgcc agcgatttat cggcaatttc gctgcccatg
     6421 cgctcgacga tatcggaggc ctgttcaagg ttgagcgaca tttcgatgat ctccgcccag
     6481 cggcgcgact cttcctccgc caactcttct tttggcatcc gcgccagata cagcttaatg
     6541 gcggtgtaga gaacgttgat atcatctgcc agcttacgca gctctttctc ctgccgtggc
     6601 tcgccgtgca tcactttatt cagcccttcc atcatctgtt ccatggcgtc gccaatgcgc
     6661 agggtttcgc gcgcggcgtt cgccagagca agcgtgggcg tgtccagcgc gctgacatcc
     6721 agatgtttag gccgtagctg ggtatccagt tccggttcat cgcgaataat cgttttgcaa
     6781 aaccgtgcca tcgggtcaac aaatggcagc atgaccaggc aacgtacaag gttgtagaag
     6841 acgtggaaat agatcaccag ttccgctttt ggcaatgaca gcttccccat tgtctctgcc
     6901 agcaaatgga caaacggcag gataatcagg ctacccacca gcttaaacag cagactaccc
     6961 agcgcgacac ggcgggctgc ggcattggcg gcactgttgt tgagcatcgc cagcaggccg
     7021 gaaccgaggt tagctccaat caccagacag agcgccacgg ggaaggagat aatgcctgca
     7081 gcggtcagag tcgcggtcag cagtacagca gcaaggctgg agtagctgat aatggcgaac
     7141 atcgcgccaa tcagcgcatc cagcagaata tcgccggtca gcgaggcaaa gatcacctga
     7201 acgccgtttg cctgggtgat cggcgttacg gcctgcacaa tcaactccag cgccagcaaa
     7261 atcagcccaa gaccaatacc gacgcggccc agttgcccgg cgcgtgactg tttgcgtccg
     7321 aggaaaaaaa tcacgccgat aaaaataagt aacggtgaca gccaggataa gtcgaaggtg
     7381 agaatacgcg ccattagcgc cgtcccgaca tctgccccca gcacaatgac cagagccggt
     7441 gcgagggcta ccagatcctg agcgacgaac gaggtcacca gcatggtggt ggcattactg
     7501 ctctgtacca gtgcggtaac gccgatcccc gcgcaaaagg cgagcggctt tttttcgacg
     7561 ctccggctaa ggacagtacg caaacgcgcg ccgaagacgc gcattacgcc ggttcgaaca
     7621 atatgagtcc cccagaccag cagggcgacg gcagaaagca ggtgaagcag cgttaacacg
     7681 agttcaggtt ctccttatcg ttatatgttc ctgaagcaat tgaatgacca aaggaccgga
     7741 aacacgctga ctggcatctt tactgtgcct tttgacattc gtttttccgg ctaagattat
     7801 tccagcctgt ttccagtata agtgtttcat attaataaag agacagggca ctcattgagc
     7861 gccctgtttg ttgtaaggaa aagtgacaga tttgtgaatc agtccgcgtc ataccccaga
     7921 ttcggtgcca gccagcgctc aacatcggag acgctcatac ctttacggcg ggcatagtct
     7981 tcaacctgat cgcgctgaat ttgcgccacc gcgtagtact tgctgtccgg atgactgaaa
     8041 taccaacccg aaaccgatgc accaggccac atagcgaaag attctgtgag tttcatgcca
     8101 gtatgttttt ccacttccag cagctcccag atggtggctt tttccgtatg ttccgggcag
     8161 gccggatagc ccggtgccgg acggatgccc tggtagtttt cgcggatcag ttcttcgttg
     8221 ctgaggttct cgttcggcgc atagccccag tagactttac gcacacgctc atggagatac
     8281 tccgcaaagg cttcggctaa acggtcggca agcgctttca ccatgatttt gttgtaatca
     8341 tcgtgctgcg cttcaaaggc atcagccagt gcgtcctctt ccagcccgcc agtcacggca
     8401 aatgcgccga tgtaatctgc tttaccagaa agcttcggcg caacgaagtc agcgagacag
     8461 tagttagcga atcccgtttt ttcggtctgt tgacgcagat ggtggctgac gttgatcaca
     8521 tgggtgcgcg tttcgtcacg gtagatttca atgtcatcgc ccacacggtt tgccgggaac
     8581 aggcccacca cgccacgcgg attcagcgtt ttctcggcgc ttaatttatc cagcatgtcg
     8641 ttggcgtctt taaacagccg ctgcgcctca acgcccacca cttcatcttc cagaatgcgc
     8701 ggatacttcc cggccagcga ccaggtcata aagaacggtg tccagtcgat gtaattacgc
     8761 agcgtttcga tgctggcttc cacttcctgc acgccgagac ggtgcgccac cggcggcgtg
     8821 taagcctgcc agtcaaatgc gaaatcgtta tcgcgcgccg cttccagcgt gaccggtggt
     8881 gtgcgtggtt tcttgcgccc gtgctgaata cgcacggttt cgtactcctt gcaggtacga
     8941 gcgacaaaat catcacgctg agtatcggaa agcagcgccg ctaccacacc aacggtgcgc
     9001 gaggcattct gcacatacac cgtagggccg ctgtagttct gctcgatttt caccgccgtg
     9061 tgcgcttttg aggtggtcgc gccgccaatc agtaacggaa tggtgaagcc ctgacgctcc
     9121 atctctttcg ccacgttaac catctcgtcc agcgacggcg tgataagccc cgaaaggcca
     9181 atcaaatcag cattcacttc tttggcgata cggagaattt tttccgcagg caccataacg
     9241 ccgagatcga caatttcgta gttgttacat tgcagcacca caccaacgat atttttaccg
     9301 atgtcgtgga catcgccttt cacggtggcg atcaccatct tgccgttggt tttaccctgc
     9361 tctttgctgg cttcaataaa cggttcgaga tacgccactg cctgtttcat gacgcgcgcc
     9421 gatttgacca cctgtggcag gaacattttg ccttcgccaa acagatcgcc gaccacgttc
     9481 atgccgtcca tcaacgggcc ttcaatcact tcaatcgggc gcgtagcctg ctggcgggct
     9541 tcttcagtat cctgctcgat aaactcggtt atgcctttga ccagcgagta ttccagacgt
     9601 ttattcacgt cccacgagcg ccactccgct tgctgggcgt tagcagtgtc gtccgcttta
     9661 ctgccgcgat atttctcggc aagctccagt aaacgctcag tgccatcgtc acgacgatta
     9721 agaatcacat cttccaccgc gtcgcgcagt tcagcgggta ggtcgtcgta aatcgccagt
     9781 tgcccggcgt tgacgatccc catatccatg ccattgcgaa tagcgtagta gaggaacact
     9841 gcgtgaatgg cttcgcgcac cggatcgttg ccacggaatg agaaagaaac gttagatacg
     9901 ccgccggaaa tcagcgcgtg cggcagttcg cgtttgatgt cttcacacgc gccgataaag
     9961 tcctgcgcgt agttgttgtg ctcttcaata ccggtggcga ccgcgaagat gttcgggtcg
    10021 aagatgatat cttctggcgg gaagccaacc tcttcggtga ggattttgta cgcccgacgg
    10081 caaatctcga ttttccgtgc gcgagtatcg gcctgtccct gttcgtcaaa ggccatcacc
    10141 accactgccg caccgtagcg acgcaacaat ttcgcgtgat ggataaaggc gtcgacgccc
    10201 tctttcatcg agatagagtt aacaatgcct ttgccctgga tacacttcag acctttttca
    10261 atgacatccc attttgagga gtcgatcata atcggcacgc gagcgatatc cggttcaccg
    10321 gcaatcagat tgagaaaacg caccatcgcc gcttcggcat cgagcatccc ttcatccatg
    10381 ttgatatcga taatctgcgc gccgttttcc acctgctgac gcgccacatc cagcgcctcg
    10441 ctgtatttct cttctttgat caggcgcttg aacttagcag agccggtgac gttggtgcgt
    10501 tcacccacgt tcacaaacag gctatcatcg ccaatgttca gcggctccag gccagacaaa
    10561 cggcaggcta cgggaatttc cggcagtttg cgcggcgcta atccttctac tgcacgactc
    10621 atcgctgcaa tatgttgcgg catggtcccg cagcaaccac cgacgatatt gagaaagccc
    10681 gcttgcgccc attcacgtat ctgttttgcc atcgtgtcgg cgtcgagatc gtattcacca
    10741 aaggcgttag gcagcccggc gttcgggtgg gcggtgacgt agcattccgc aatccgtgat
    10801 agttcctgca cgtactggcg cagttcatcg ggccccagcg cacagttcag gccaaaggtc
    10861 agcgcttcgg cgtggcgcaa tgagttgtaa aatgcttcgg tggtctgccc ggagagcgtg
    10921 cgcccggagg cgtcggtgat ggtgccggag atcataatcg gcagctcaac gcccagcgct
    10981 tcaaactccg ttttcaccgc aaagaccgcc gctttggcgt taagggtgtc gaaaacggtt
    11041 tcaatcagga tcagatccgc accaccttcc accagcgctt tggtggattc gcgataggcc
    11101 gccaccagct ggtcaaaagt gatgttgcgg aatgccggat cgttgacgtc cgaagaaata
    11161 gacgccgtgc ggttggttgg gccgagaaca ccggcaacgt agcgcggttt ctccggcgtg
    11221 cgcgcggtcc actcgtcagc acgggcgcgc gccagtttcg ccgccgcaaa gttgatttcc
    11281 gccgacaggg attccatctg gtaatccacc atcgcaatgg tcgtggagtt gaaggtgttg
    11341 gtttcgatga tatccgcgcc cgcttcaaag taggcgttgt ggatagcggc gatcacttcc
    11401 ggtttactga gtaccagcag gtcgttgttg cctttgaggt cgcatggcca gtcggcaaag
    11461 cgttcaccac gaaaatcggc ttcgttcagt cgatagctct ggatcatggt gcccataccg
    11521 ccgtccaaca ccagaatacg ttcatttaac tgcgcacgca gttgttccac tttgctgctc
    11581 acacttgctc ccgacacgct caacccagat ttaataaaaa ttcaacaaac catactggca
    11641 taaacgcatc tgtggtaaaa gcgaccacca cgcaacatga gatttgttca acattaactc
    11701 atcggatcag ttcagcaact attgcattag ctaacaataa aaatgaaaat aatttccatg
    11761 atacagaaaa aggaggctgt catggtcgcc cccattcccg cgaaacgcgg cagaaaaccc
    11821 gccgttgcca ccgcaccagc gactggacag gttcagtctt taacgcgtgg cctgaaatta
    11881 ctggagtgga ttgccgaatc caatggcagc gtggcactca cagagctggc gcaacaagcc
    11941 gggttaccca attccacgac ccaccgcctg ctaaccacga tgcaacagca gggtttcgtg
    12001 cgtcaggtcg gcgaactggg acactgggca atcggcgcac atgcctttat ggtcggcagc
    12061 agctttctcc agagccgtaa tttgttagca attgttcacc ctatcctgcg caatctaatg
    12121 gaagagtctg gcgaaacggt caatatggcg gtgcttgatc aaagcgatca cgaagcgatt
    12181 attatcgacc aggtacagtg tacccacctg atgcgaatgt ccgcgcctat cggcggtaaa
    12241 ttgccgatgc acgcttccgg tgcgggtaaa gcctttttag cccaactgag cgaagaacag
    12301 gtgactaagc tgctgcaccg caaagggtta catgcctata cccacgcaac tctggtgtct
    12361 cctgtgcatt taaaagaaga tctcgcccaa acgcgcaaac ggggttattc atttgacgat
    12421 gaggaacatg cgctggggct acgttgcctt gcagcgtgta ttttcgatga gcaccgcgaa
    12481 ccgtttgccg caatttctat ttccggaccg atttcacgta ttaccgatga tcgcgtgacc
    12541 gagtttggcg cgatggtgat taaagcggcg aaggaagtga cgctggcgta cggtggaatg
    12601 cgctgacttt ttctggcggg cagaggcaat attctgccca tcatacctga gtggcaatag
    12661 aataagggtg tctgttaatc gcattgacgc caaaataact taatgtcata cacttcacac
    12721 acttttatta actcagcatt atttttaaac atcaaaccac ttaattatta acaaaatcgc
    12781 aagatctcat tccaccgaaa cttttacggc tgcattatga gattgagcga tttatgatat
    12841 ttttttagtt gtaatcatat taaatttttt cactgttctg atagttaaaa ttcaagacat
    12901 caataaacaa tgagatattt aaatgattac tcgtattcct cgtagttctt tctctgcaaa
    12961 tattaataat acagcccaga caaatgaaca ccaaaccctg agtgaattgt tttataaaga
    13021 actcgaggat aaattttctg gcaaggagct ggcgacgcct ctattaaaaa gcttctcaga
    13081 gaactgtaga caaaatgttc gtcatatctt tagcaacaag gattttgtca ttaaattttc
    13141 cacgtccgtc ttacaagctg ataagaaaga aattacgata attaataaaa acgaaaacac
    13201 gacactcact caaaccattg ctacaatatt tgaagaatac ctaattgaaa ttttacctca
    13261 acgctcagac actcttgata aacaagaatt aaacctaaaa tcagatagaa aagaaaaaga
    13321 attcccaaga attaaactta atggtcaatg ttattttccg gggcgcccca aaaccgtata
    13381 gtataccgac acattgctgc acaatatatt aatgatattt atcagaatgt tgattacaaa
    13441 ccccatcaag atgattactc ttcagctgaa aaatttctca ctcacttcaa caaaaaatgc
    13501 aaaaaccaga ctttggcgtt gatttccagc cgtcctgagg ggcgttgcgt tgctgcctgc
    13561 ggtgatttcg ggctagttat gaaagcatat tttgacaaga tggaatcaaa tggcatcagt
    13621 gttatggcag ccatattact ggtggataac catgctttga cggtccggct aagaataaag
    13681 aacacaactg aaggatgtac ccattacgtg atttcggttt atgatcctaa tgtaactaac
    13741 gataaaataa gaattatgag cgaaagcaaa gaggatatta aacactattc tctgatggat
    13801 tttatgaatg tagattatag cctcctgaaa tggtcaaatg atcatgttat taaccaatct
    13861 gttgcaataa ttccagcact tccgaaagaa cagctattga tgttaaaagg aactgtggat
    13921 gaaataaccc ctccattatc accagcaacg atgaatttgc taatggcaat tggtcagaat
    13981 caccaactta cgcaactgat gattcagctc cagaaaatgc cagaactaca tagaacagaa
    14041 atgttgactg cctataatag tattaacctc cccggtttat atttggctat aaattatggc
    14101 aatgcggata tcgttgagac tattttcaat tcattgtcag aaacaggata tgaagggtta
    14161 ctctcgaaaa aaaatctcat gcatattctg gaggcaaaag ataaaaatgg tttttctgga
    14221 ttatttttag cgatatcacg taaggataaa aatgttgtaa cctcgattct gaacgtctta
    14281 cctaaactgg ccgcaacaca tcatttagat aatgaacagg tgtataaatt cctgagtgcc
    14341 aaaaatagaa cgtccagcca tgttttatac catgttatgg cgaatggtga tgccgacatg
    14401 ctgaaaattg ttttggtcgc gttacctttg ttaattcgca catgtcattt gactaaagaa
    14461 caggtactcg atctcctgaa ggcaaaggat ttttatggtt gcccaagact atacctggcg
    14521 atgcaaaatg gacatagcta tatcgtgaaa gttattctcg aagcattgcc ctgtctggcc
    14581 caggaaatta acatttcagc ttccgatatt gtagatcttc tgaccgctaa aagtcttgcg
    14641 cgcgacacgg gtttgtttat ggccatgcag cgcggacata tgaacgttat taatactatt
    14701 tttaacgcat tacccactct ttttaatacg tttaaattcg ataaaaaaat atgaagcccc
    14761 tcctcctggc aaataattct aatgaatatc ccggtttgtt ttcagcgata cagcataaac
    14821 aacaaaacgt tgtagagacg gtttatcttg ctttatctga ccatgcacgc ctgtttggat
    14881 ttaccgctga agatattatg gatttttggc aacacaaagc gccacaaaaa tactctgcct
    14941 ttgagttggc ttttgaattt ggtcaccggg ttattgctga attaatcctt aatacattaa
    15001 ataagatggc tgaaagcttt ggctttacgg ataaccctcg atacattgcg gagaaaaatt
    15061 atatggaagc tttactcaaa aaagcatctc cccataccgt acgctaaatc tttgcctgcg
    15121 ccgatacgca taaacatctt ccacatgccc ttcacgtatg cggttttgta gtgcgcgcca
    15181 gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac agcggaccaa tacgcgggtc
    15241 ggcgcatagc cagtggcgaa actcttccgg gaaaacatcg cccggcgaga cgctgtacca
    15301 cggttcgctg gcaagttcgt cttccggata gcgcggcggc gggatgtcgc ggaaattcac
    15361 ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc acacgcccgt gacgggtgac
    15421 accgaagttt ttaaacagca tgtcgccagg gaaaatgtta gcagcggcaa gctggcgaat
    15481 agcgttaccg tattcttcaa tggcgtcgcg caactgctga ccttccactt gttccagcca
    15541 gatattgagc ggcaccatcc gccgctcgat atagagatgg cgaattacaa tctgctcacc
    15601 aagatcggtg attttttccg ctgcttcctg gagcagtaat tccattaata ccggggaaat
    15661 atgccgcttc tccagcacaa agttttcaaa ctcctgggtg tccgccattc ggcccacacg
    15721 gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga gcggcagaca tctctttctg
    15781 cggcgcgaac ttgtctttga tgactttgaa tacccgatca aagcccggca gcgtaaacac
    15841 caacatcacc attccacgaa tacccggcgc ttcaatgaac tgctcattac agccctgtag
    15901 ataaacgaga tattcgcggt agctttcggt tttggcgtgc ttctggcagc cgatagccat
    15961 atacaattca gcagtggttt tacctggcag aatttcccgt agccactcga ccagcgctgc
    16021 gggcagcggt gcataaacca taaaataaga acgcgcaaag ccaaaaacaa tgctcgcttc
    16081 ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg tcgtccgtct ggtggatcgg
    16141 cagtaagaag ggcaacgtgc cggaaggcgt gatcagtttg cccaccagcc aggcggcttt
    16201 attgcggtaa aacagttcgt tcgccacctg taaatgactt tccgcgaggt tgtctgtccc
    16261 cagcgtttcc gtcagatggc gaatgatgta atgaatgtca cggcttttat tctgccagcg
    16321 caggcgcagc ggcaggtcgc tgataacgcg catcagtaga gattcccagc cgtgatcggg
    16381 gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag cggcgctctg gctgagagct
    16441 aaagataaaa agccgctcgg gagtaagcga gcggtggtca aataaccgac agtacacgga
    16501 gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc ggcaacagcc gcgtgtaatg
    16561 ctctttaacg cgcagtaaaa atgccgcgtc cgtactttgg ccgttagtaa tgcagcgcag
    16621 ttgctccacg accagaccaa cgtgatgatc gtaaagatgg atacggtttt tcatcgcctg
    16681 ctggacagca tgccagtcgg cctgttcgaa acgctgctgc gccccggaag tcacttcgag
    16741 gaatcgacca tactgagcgt cgaagccttg caaaatggtt tgagcaatca gtaattccag
    16801 gccacgcggc atttagcgca ctcatcagga gcagagaatt gccggatgcg gcgtaaacac
    16861 cttatccagc ctacgttcgg caacggttgt aggcatgata agacgcgcaa gcgtcgcatc
    16921 aggcatcgtg caccgattgc cggatgcggc gtaaatgcct tatccggcct acagtcagca
    16981 acgtttgtag gttgcttaga actgcgattc ttcagtggag ccagtcagcg cggtgactga
    17041 agacgtgccg ccctgaataa tagtcgtcac tttatcgaag taacctgtac ccacttcctg
    17101 ctggtgagat acgaaggtat agccatcttt cgcggcggca aattccggct gctgcacttt
    17161 ctcaacatag tgcttcatac tttcgccctg cgcataagcg tttgccaggt cgaacatgtt
    17221 aaaccacatg ctgtggatac ctgccagggt gatgaactgg aacttgtagc ccatatccga
    17281 caactgctgc tggaagctgg caatagtttt gtcgtcgagg tttttctgcc agttgaacga
    17341 cggcgagcag ttataagcca gcagtttgcc cggatatttc gcgtggatag cttgtgcaaa
    17401 gcgacgcgcc agttccagat ccggcgtgga ggtttcacac cagaccaggt cagcatatgg
    17461 cgcatacgcc aggccacggc tgatcgcttg ctcaatgccc gcatgagtac ggaagaagcc
    17521 ttcactggta cgctcgccgg taataaattc gctgtcatac gggtcgcaat ctgaggtgat
    17581 cagatccgcc gcatcagcat cggtacgtgc aaccagcagg gttggaacgc ccgtcacgtc
    17641 agctgccaga cgcgccgcga ccagtttctg aatagcttcc tgagttggca ctaaaacttt
    17701 gccgcccatg tgaccgcatt tcttcactga cgccagctga tcttcgaagt gaactgccgc
    17761 agcaccggct tcaatcatcg ctttcatcag ttcaaaggca ttcaggacac cgccaaaacc
    17821 ggcttccgca tcggcaacga tcggcaggaa gtaatcgaca tagcgcggat cgcccggctc
    17881 aatgcccacg gaccattgga tctgatcggc acgacggaag gtgttgttga tccgctccac
    17941 cacagctggc accgagtttg ccggatagag cgactgatcc ggatacatgc tggccgccag
    18001 gttagcgtcc gccgctacct gccatcccga cagatagact gcttcaatac ccgctttcgc
    18061 ctgttgcagc gcctgaccgc cagtcagtgc accgaggctg ttgatgtagc cttttttcga
    18121 ctcaccgtgc agcagacgcc acattttcgc tgcgcccagt tgcgccagcg tgcattcagg
    18181 attgactgaa ccgcgtaatt tcaccacatc ttccgcactg tatgggcgag taatgccttc
    18241 ccaacgcggt tgagtccact ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat
    18301 gtgcagatgc tccatagtta tgtggtggtt tacgctaaca ggcggtagcc tggcagggtc
    18361 aggaaatcaa ttaactcatc ggaagtggtg atctgttcca tcaagcgtgc ggcatcgtca
    18421 aaacggccct gggagaaacg ttcttcgccc agttcgctgg caatgacttt catctcttcg
    18481 cccagcatct ggcggaacaa ggctttggtc accggtttgc cattgctcaa cgttttttga
    18541 tgatggatcc actgccagat cgaggtacgg gaaatttcag ccgtcgccgc atcttccatc
    18601 aggccataaa tcggcacgca gccgttgccg gagatccacg cttcgatgta ctgcacagcc
    18661 acgcgaatgt tggcgcgcat accttcttcg gtgcgttcac catcacaagg tgccagcagc
    18721 tgatcggcag taatcggcgc gtcttgttcg cgcaatactt caagctgatt tttacgggag
    18781 ccgagaatat cgttgaatac cgccattgcc gtgtccgcaa ggcctgggtg agcgatccat
    18841 gtgccatcgt gaccgttatt ggcttccagc gctttatccg cttttacttt gttgagcacc
    18901 tggttattac gctcttcatc tttgctcgga ataaacgctg ccatgccgcc catcgcaaaa
    18961 gcaccgcgtt tatggcaggt tttaatcagc aggcgtgagt aagcattcag gaatggttta
    19021 tccatcgtca cggcctgtct gtctggcagg acacgatcgg gatagttttt caacgttttg
    19081 atatagctga agatgtaatc ccagcgaccg cagttcagac caacaatatg gtcgcgcagc
    19141 gcgtgaagga tttcgtccat ctggaacacc gcgggcagcg tttcaatcag caacgtcgcc
    19201 ttgatggtgc cgcgcggcag attaaagcga tcttctgcat agctgaagac ttcgctccac
    19261 caggccgctt cctgccagga ctgggttttc ggcagataga aatagggacc gcagcccttt
    19321 gccaacagtg cctgatagtt gtggaagaaa tagagcgcaa aatcaaacag gctaccgggg
    19381 attgcctcac cacgccaggt gacatgtttt tccggcaagt gcagaccgcg tacccgacaa
    19441 atcaaaaccg ctggattggg cttgagctga taaattttgc ctgcttcatt ggtatagctg
    19501 atggtaccgt taaccgcatc gcgcaggtta atttgcccgt cgatcacttt gttccagtct
    19561 ggtgccagtg aatcttcgaa atcggccata aagactttca cattggcgtt cagtgcgttg
    19621 atcaccatct tgcgctctac cgggccagtt atctctacgc ggcggtcttc taagtccgca
    19681 ggaatcccgc gaattttcca atcagcatcg cgaatggaag ccgtttccga aataaaatca
    19741 ggcaacgttc cgttatcaat atcttgctgc tgctgaatgc gcgctgccag aagtttattg
    19801 cgttgtggcg taaaatgcgt caccagctca gtcagaaatt ctaccgcttc ggcagtaaga
    19861 atttgcttct cctgctcgcc atacggcctt gtgaaagcca gttcatcggt tgttgttgcc
    19921 tgttcagtca tcgtgcagct cctcgtcatg gatccgaaaa cttcccctct gtgaacgaag
    19981 gaatgttgtg cgcttttcgt tcagcacaac taagactact catttaaaat gcaaaaacaa
    20041 aaacaatttc cattttaatt aaaaatactt gataacttat tgataacaat atgaataaaa
    20101 tttattcact ttctgaggtg cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt
    20161 aaggtgctga atcgcttaac gatcgactat cacagaagat taatccagcg ttggattcat
    20221 gtgccgtaga tcgtatggcg tgatctgata gacgtaatag ttgagccagt tggtaaacag
    20281 caaattaccg tgactacgcc agctcgctcg cggtttgttt tgcggatcgt tgtgcgggaa
    20341 atagttatac ggtacatccg ggtctagtcc ggcttccaca tcgcggaaat attcctgcgc
    20401 cagcgtttgc gcatcatatt cgggatggcc cgtcacaaag gcaatgcgct tatctttgct
    20461 ggcaaacaga tatgcatccc cttcttccgt ctctgccaga atttccagat cggtgtaatc
    20521 acgaatcaac gctgccggaa agtcagcata gcgcgaatgc ggtgccagga atgaatcatc
    20581 aaagccacgc gtcagaagcg catgaggatg gagaatatga tgctcgtaaa cgccagagag
    20641 tttgtcggtg cgagtttgct taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc
    20701 ccagcagaca aacagcgtcg aggtgacgtg atctttcgac cactccagca cctgtttgat
    20761 ctgcggccag taagcgacat cattaaactc caccaggccc agcggcgcac cggttacaat
    20821 cagaccgtca aagttctgtt cctgaatatc ttcaaagtta cagtagaagt tgttcagatg
    20881 ctctgcgggc gtgttgcgcg attcacggga atcgatgcgc aacagctgaa tatcgacctg
    20941 caaaggtgag tttgaaagca ggcgcagaaa ctgattttca gtttcaatct tcttcggcat
    21001 caggttaagg atcagaacct taagtggacg aatttcctga ccagacgcac gagaagttgt
    21061 catcacaaac acgttttctt cacgcaagaa attgacggcg ggtagctcgt ccggcacacg
    21121 aatcggcata acctgattac ctcactacat acgcttatac gtttagacat ccagatagct
    21181 gaagataacc agaaaatttg ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa
    21241 ggaaaaggct acgttagaat ataagaatga cgaaaaggag agaaaatggt tattagtatt
    21301 cgccgctcac ggcatgagga aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat
    21361 gccacccacg attttttatc agcagagtat cgggccgagc tggaagagct ggtgcgttcc
    21421 tttctgccgg aagcaccgtt gtgggtcgcg gttaatgaac gggagcagcc ggttgggtct
    21481 atgttgttaa gtgggcagca tatggatgcg ctgtttatcg atcctgatgt gtgcggctgc
    21541 ggcgtgggtc ggatgctggt ggagcatgcg ctctcgatgg caccggaact gacaaccaac
    21601 gttaatgagc aaaatgagca ggcggttggg ttctataaga aggtgggttt taaggttacg
    21661 ggacgctctg aggtggacga tttggggaaa ccgtatccgt tgctgaatct ggcgtatgtg
    21721 ggggataagc cgcccctgcc gtgcctgccc aagctggaga agacgcgcca gcgtcgcacc
    21781 cggcattttt ttcaccaacg caaaaaggcc atcctgacgg atagcctttc gcttaatttg
    21841 atgcctggca gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc
    21901 gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctacgg ccgccaggct
    21961 aattctgttt tatcagaccg cttctgcgtt ctgatttaa
//
LOCUS       DAJTXY010000062        21508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTXY010000062 DAJTXY010000000
VERSION     DAJTXY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.062"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(9..1007)
                     /gene="fbp"
                     /locus_tag="OO566_002406"
     CDS             complement(9..1007)
                     /gene="fbp"
                     /locus_tag="OO566_002406"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6605659.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1183..2556
                     /gene="mpl"
                     /locus_tag="OO566_002407"
     CDS             1183..2556
                     /gene="mpl"
                     /locus_tag="OO566_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6605660.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLHD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2639..3190)
                     /locus_tag="OO566_002408"
     CDS             complement(2639..3190)
                     /locus_tag="OO566_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6605661.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3284..4636
                     /gene="pmbA"
                     /locus_tag="OO566_002409"
     CDS             3284..4636
                     /gene="pmbA"
                     /locus_tag="OO566_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6605662.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4819..5205
                     /gene="cybC"
                     /locus_tag="OO566_002410"
     CDS             4819..5205
                     /gene="cybC"
                     /locus_tag="OO566_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6605663.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5250..5714)
                     /gene="nrdG"
                     /locus_tag="OO566_002411"
     CDS             complement(5250..5714)
                     /gene="nrdG"
                     /locus_tag="OO566_002411"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6605664.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5872..8010)
                     /gene="nrdD"
                     /locus_tag="OO566_002412"
     CDS             complement(5872..8010)
                     /gene="nrdD"
                     /locus_tag="OO566_002412"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6605665.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8404..10059)
                     /gene="treC"
                     /locus_tag="OO566_002413"
     CDS             complement(8404..10059)
                     /gene="treC"
                     /locus_tag="OO566_002413"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6605666.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10109..11530)
                     /gene="treB"
                     /locus_tag="OO566_002414"
                     /pseudo
     CDS             complement(10109..11530)
                     /gene="treB"
                     /locus_tag="OO566_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11649..12596)
                     /gene="treR"
                     /locus_tag="OO566_002415"
     CDS             complement(11649..12596)
                     /gene="treR"
                     /locus_tag="OO566_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6605667.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12781..12834
                     /gene="mgtL"
                     /locus_tag="OO566_002416"
     CDS             12781..12834
                     /gene="mgtL"
                     /locus_tag="OO566_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6605668.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12975..15671
                     /gene="mgtA"
                     /locus_tag="OO566_002417"
     CDS             12975..15671
                     /gene="mgtA"
                     /locus_tag="OO566_002417"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6605669.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15877..16263)
                     /gene="ridA"
                     /locus_tag="OO566_002418"
     CDS             complement(15877..16263)
                     /gene="ridA"
                     /locus_tag="OO566_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6605670.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16336..16797)
                     /gene="pyrI"
                     /locus_tag="OO566_002419"
     CDS             complement(16336..16797)
                     /gene="pyrI"
                     /locus_tag="OO566_002419"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6605671.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16810..17745)
                     /gene="pyrB"
                     /locus_tag="OO566_002420"
     CDS             complement(16810..17745)
                     /gene="pyrB"
                     /locus_tag="OO566_002420"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6605672.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSGNTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17749..17883)
                     /gene="pyrL"
                     /locus_tag="OO566_002421"
     CDS             complement(17749..17883)
                     /gene="pyrL"
                     /locus_tag="OO566_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6605673.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18164..18559)
                     /locus_tag="OO566_002422"
     CDS             complement(18164..18559)
                     /locus_tag="OO566_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6605674.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18691..19404)
                     /gene="bdcA"
                     /locus_tag="OO566_002423"
     CDS             complement(18691..19404)
                     /gene="bdcA"
                     /locus_tag="OO566_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6605675.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19475..20068
                     /locus_tag="OO566_002424"
     CDS             19475..20068
                     /locus_tag="OO566_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6605676.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20213..20665
                     /gene="tabA"
                     /locus_tag="OO566_002425"
     CDS             20213..20665
                     /gene="tabA"
                     /locus_tag="OO566_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6605677.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20788..>21508
                     /locus_tag="OO566_002426"
     CDS             20788..>21508
                     /locus_tag="OO566_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6605678.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5201 a         5329 c         5656 g         5322 t
ORIGIN      
        1 gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc aaccatatgg
       61 tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat atccagaata
      121 cgctctttgc catcgctcgc tttaccaccc gcttgttccg ccaggaatgc catcgggttg
      181 cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg gtagagataa
      241 ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat ataacgcgag
      301 gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta cttcttcacc
      361 ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc tttctccggg
      421 aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta agtaaaggcg
      481 tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac cacgtaacct
      541 gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg cgtgccaaca
      601 ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac atcgatgttg
      661 gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca gccttcaaag
      721 acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg tgctttcagt
      781 gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc gccctgcacg
      841 ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat atcgcgatgg
      901 ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc ggtagcatga
      961 gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa actttccctg
     1021 caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa tgttgcgctc
     1081 aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac ttgcccgaca
     1141 tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat tcatatttta
     1201 ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt aggccatgaa
     1261 gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga gaagcaaggc
     1321 attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga tctggtgatt
     1381 attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga aaaaaacatc
     1441 ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcacgaccg ctgggtgcta
     1501 gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg gattctggaa
     1561 cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt tgaggtttcg
     1621 gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga ctgcgccttc
     1681 ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct caacaacctt
     1741 gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca gttccaccat
     1801 ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga catcaacctg
     1861 aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga gcagggtcac
     1921 tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct ggatggcgaa
     1981 aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa tggcctgatg
     2041 gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa cgcgctgggt
     2101 tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg cgtcacggta
     2161 tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc gctgcgtggc
     2221 aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa taccatgaaa
     2281 atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga agtcttcctg
     2341 ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg cgttcagcct
     2401 gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac cgctcagcct
     2461 ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca gaaactgctg
     2521 gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct gatatagcat
     2581 tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac aacgaaaatt
     2641 acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg gatttcggcg
     2701 gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt tgctgacgat
     2761 cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga tcgatcagac
     2821 gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga cggtttttca
     2881 gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc ttaccgatga
     2941 gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca atagccgggc
     3001 gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca acaatttccg
     3061 cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg acccagataa
     3121 tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag tcttcgggct
     3181 gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa gatgcgcact
     3241 gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac ttgcaatgaa
     3301 agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt ccactgcgct
     3361 ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga ccaccggcat
     3421 tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg atggcgcgct
     3481 ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg atttaagccc
     3541 gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata cctcgccaga
     3601 tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga atctcgactt
     3661 gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc gcgcagaaca
     3721 ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct ttaacagcca
     3781 ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact gctcaacgcg
     3841 tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc gtgattacgc
     3901 ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg gggccgactg
     3961 tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga aagcgccggt
     4021 catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg cgatagcggg
     4081 tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac aaattctgcc
     4141 ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt cgacgccatt
     4201 cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca tcctgactca
     4261 gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg gacatgcggg
     4321 cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa tgctcaaaga
     4381 gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg ccattaccgg
     4441 tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc agtatcccgt
     4501 tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg tcaccgtcgg
     4561 taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc cggagatgaa
     4621 aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa aaattacaca
     4681 aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg tactcgcagt
     4741 acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat gaagtgtaaa
     4801 taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt ctcctcgttg
     4861 gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac cctcaacgac
     4921 aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc gttaacgaag
     4981 atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga agataaatca
     5041 ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt cggtcagatt
     5101 gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc tgctgcagag
     5161 caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct catttccctg
     5221 ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac ctgattgctg
     5281 ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc gtcgaccagc
     5341 acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag tttatagcct
     5401 gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac cagtttcaga
     5461 atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat cccctggcgt
     5521 ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt ggtaaatggc
     5581 tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc atgaacacac
     5641 ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac aggatagtac
     5701 tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc atccggtaat
     5761 caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc aaatgtaggc
     5821 cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag attaacctat
     5881 ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac cagcgttaaa
     5941 cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg acacacggga
     6001 ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact cgaactcacc
     6061 ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat acggtacatg
     6121 ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa tgtttggata
     6181 ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt caaagtcgat
     6241 cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg tgtagtaacc
     6301 tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc agaagcggtc
     6361 gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt cttctttcca
     6421 ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc gaagcttctc
     6481 gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga tgccgatgta
     6541 acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat cgtcagcatt
     6601 cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg ctttcacgcc
     6661 ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac gttcatccag
     6721 cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg gcaggttcag
     6781 gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt cattttccca
     6841 cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga ctttcactac
     6901 ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca gcgccagctg
     6961 tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa tcgcaaacac
     7021 cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac ggttacgcag
     7081 gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac caaaggttac
     7141 aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca gcgactggaa
     7201 ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt ccgggatgtt
     7261 ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg gcgccagcac
     7321 ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct gagcaataat
     7381 ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct cggcgttccc
     7441 cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt tgaacatcgg
     7501 gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat gtgcctgcac
     7561 cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc cggccagcag
     7621 gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga gtaacgaggc
     7681 gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt tttcacgttc
     7741 aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt atggacccga
     7801 catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt tgcgcccctg
     7861 catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat cgacttccgc
     7921 tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg gcactttgca
     7981 gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag aacaggttat
     8041 ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg tgctttccgc
     8101 aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt agtagcaatt
     8161 atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata tatagtgctt
     8221 tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca gagcgtacgg
     8281 atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg aaaaatcaac
     8341 aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg gcttagggaa
     8401 gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg cacagggttg
     8461 tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca tttgccctgg
     8521 ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt gccccttcca
     8581 ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt aattgcccca
     8641 tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt aaaacacaga
     8701 ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc ctaggccaat
     8761 ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca tgggcgtgcg
     8821 gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat cacgaccatc
     8881 gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag taatgcgcgt
     8941 gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg ttccctgcat
     9001 gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac cttcatcacc
     9061 aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat tccatgtcac
     9121 gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa agtcaggttt
     9181 agccaacgtc catttttcac caccgggata atcgaccttc aggtgatgaa aattaaaggt
     9241 catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa ggctggtgga
     9301 ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt tcatctcgtg
     9361 caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc catccaggtc
     9421 ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca acccgtcgac
     9481 cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg ctggattctc
     9541 ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt cgctttccgt
     9601 atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg gcgtttctgg
     9661 ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca gcgcctcgcg
     9721 aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga tacgaatccc
     9781 gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt aggtgggatc
     9841 aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga cataaaaggg
     9901 ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt ggataacgcc
     9961 acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat aaatctggta
    10021 gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc tgcaaatgcg
    10081 agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc aggcggtatt
    10141 tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata gccattgcca
    10201 gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg ccccctacgc
    10261 cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga ccagaaccaa
    10321 tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt gcaggctcag
    10381 tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca ttgtgcttgc
    10441 ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac agcgcaatca
    10501 gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata gcaagggtgg
    10561 tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat gccgcaccaa
    10621 tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca tcgccaatca
    10681 tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc agcgaacaga
    10741 cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga gtttcaataa
    10801 cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag cccactttgg
    10861 cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc agataagcgt
    10921 tcatcagctg ggaggaaacc agtgtcacgc caagcacgat accaaggatc ggcgtgccgc
    10981 ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa atcgcttcac
    11041 cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc gccagcgtct
    11101 gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc aaaccgccgc
    11161 taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc aactgctcat
    11221 gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc tgcgcctgtc
    11281 cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc accacctgaa
    11341 attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt tctttcggtc
    11401 tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg ctcaccgtcg
    11461 caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt tggtttattt
    11521 tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa tatattgcgc
    11581 ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat ttatcgttca
    11641 ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct gcgcccggtt
    11701 acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc aggatctacg
    11761 gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct cgccagttgc
    11821 aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag cgtgtcggtt
    11881 gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc atagccttgc
    11941 ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt gcagaacgcc
    12001 aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac gccgagataa
    12061 ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat tgccccttcg
    12121 tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac cagcgatgac
    12181 tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac tacgccgtcg
    12241 atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga aaactgattt
    12301 tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt ttgaacggcg
    12361 agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt atcgctttgc
    12421 ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat cactgcttca
    12481 acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg ggaaaccgta
    12541 gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt ttgcatcctg
    12601 ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca ttcagcaatg
    12661 ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc cataaatgct
    12721 acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac cggaggttat
    12781 atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg gtaagcccgt
    12841 cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct gctcagcttt
    12901 attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt tcagacagtg
    12961 cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat ttaccttccc
    13021 gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg gcggtcccgc
    13081 cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa ttgtggaaaa
    13141 cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc cgcgaacaac
    13201 atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat ttatgggtct
    13261 gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct tacgccacgg
    13321 aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg ttgctgaact
    13381 ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg gtcagtaata
    13441 ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg gagatcccga
    13501 tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg atcccggcag
    13561 atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta accggtgagt
    13621 ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat ccgctggagt
    13681 gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa gcaatggtga
    13741 ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt gagcaggaaa
    13801 gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg attcgcttta
    13861 tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc gactggtggg
    13921 aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg ttgccgatga
    13981 ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa gtgatcgtca
    14041 aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact gataaaaccg
    14101 gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc ggtaaaacca
    14161 gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga cttaaaaatc
    14221 tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg ctggccagcc
    14281 gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg tcggtggtag
    14341 tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag gaaatcctca
    14401 atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac accatgctgc
    14461 gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg gttgcggtgg
    14521 cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa tccgacctga
    14581 tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct ccggcactga
    14641 aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt gagttagttg
    14701 cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt ggtagtgata
    14761 ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg ctgtttgccc
    14821 gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa gggcatgtgg
    14881 ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg gatatcggca
    14941 tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc ctgctggaaa
    15001 aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc gccaacatgc
    15061 tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc gtgctggtgg
    15121 cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt cagaacctgc
    15181 tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag caaattcaaa
    15241 agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc ggaccgatca
    15301 gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat gccaacacgc
    15361 cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg tcgcaaacgc
    15421 tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt gcatcgtggc
    15481 cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg ttttcaccgc
    15541 tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg ctggttgcga
    15601 ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc cgtcgttacg
    15661 gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag acaaagtgag
    15721 ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc aataaattgc
    15781 aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt gtcatcagac
    15841 ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag cgattgcttc
    15901 gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac gtgccgggaa
    15961 agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg cgaagtcgtt
    16021 cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc cagcggcttc
    16081 gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt ctgccggtac
    16141 ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat tgcccagatc
    16201 aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga tagttttgct
    16261 catgatttct cctttattac agcggtaaaa agtctggctc attataggga gccagacttt
    16321 tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt tttcacagta
    16381 tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg atgaaaccgg
    16441 ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga tgcgctccgg
    16501 cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa ccgtggcttg
    16561 cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat tttcgatttt
    16621 gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg tgatgcgctg
    16681 atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg cggggatatg
    16741 gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt gtgtcatctc
    16801 tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg cctggcgagc
    16861 gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat ccacatccgt
    16921 cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt tggcgttgtg
    16981 gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg acgggtccag
    17041 acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca cttcttcaat
    17101 agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa tgtattgcgg
    17161 cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact tcgctagcgc
    17221 ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg cgacgtggag
    17281 attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg tttgcgtcgg
    17341 atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa attcggtggc
    17401 caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt aagtgctgat
    17461 aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat tgccgctgtc
    17521 ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag agaggcgggt
    17581 acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca gctctggttg
    17641 cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat cgcgactaag
    17701 gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt atctcctgac
    17761 gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca acgggaagaa
    17821 aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa cacactgaac
    17881 catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg gtgcaagcat
    17941 ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa aatatatgca
    18001 tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat tgcagacatt
    18061 tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc cattcatccg
    18121 acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc tcagggatac
    18181 gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc accgccgtcc
    18241 agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg tcttcaaatt
    18301 gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa gtgcatcccg
    18361 cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc tgaaaatcgg
    18421 gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc aaatcgccgg
    18481 aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca gcagggaaaa
    18541 cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca gaagatttat
    18601 atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag acatcatgga
    18661 taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat caatggtatg
    18721 catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca taccagcgac
    18781 ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat cgcgcattgg
    18841 cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt tgatggtgat
    18901 ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg cagatttact
    18961 ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg agccgatgat
    19021 taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat gataaggagc
    19081 atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca gggcatcgcc
    19141 aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct tacgaacgac
    19201 atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc cagtctcttg
    19261 tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat tggccccatc
    19321 ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga ggatgagaac
    19381 tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac atgcattaca
    19441 aaactgtttt aactttctgt caacaggttt tgtaatggtt actaaaaaac aatctcgcgt
    19501 tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa aagtgctttt
    19561 tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc ttggtattaa
    19621 ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc gtgtactcaa
    19681 tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg atcgtccggt
    19741 aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc aaaacggcgg
    19801 ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag cgcgtgatat
    19861 tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg ccaggcggca
    19921 tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag ggctttctgc
    19981 gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc tggcggggga
    20041 agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc gggaacgcgc
    20101 ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga attgatcatc
    20161 ttttcgtata acgatagaaa tgaaacattg ttttaattaa ggagtggaaa agatgatcat
    20221 cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg cgattgagca
    20281 tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg aaggcaatcg
    20341 tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc gtgcggagta
    20401 ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca tgaccttcag
    20461 cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca tcgcattttt
    20521 gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg ttgtgtttta
    20581 tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg ttcgcaaagc
    20641 agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca ggtttacggc
    20701 gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt aatgcttaga
    20761 cgttcttatt aactcaagga gttcgtcatg agcaaaatat cagatttgaa ttattctcaa
    20821 cacattacat tagccgacaa ttttaaacaa aaaaatgaag ccttagatac ctggtatgtg
    20881 ggaatgaatg attttgcccg aattgccggg gggcagaata gcagaagcaa tattctttct
    20941 cctagagcat ttttggagtt tttggcaaag atatttaccc taggttatgt ggattttagc
    21001 aaacgctcca acgaagcggg tagaaatatg atggctcata ttgagttctc atcttattct
    21061 aaagatactg atggcaatga aaaaatgaag ttttacatga ataatcctga aggggagcga
    21121 gcggatttat ctaaggtgaa gatagaaatt acacttgcct cagcttctac taaagggatt
    21181 cgtgaaggac atacagtcat tatatttaaa caatctgatg gttcgactaa tcgttatgaa
    21241 gggaagtctt ttgaaagaaa agatgatagt tcattacacc tgattactaa caaggttctg
    21301 gcgtgttacc aacgcgaagc taacaagaaa atagcgcggc tattaaataa tcatcagaag
    21361 ttaaataata tacaggagtt aaatgattcg caggagttaa ataattcaca gaagttaaat
    21421 aattcgcagg agttaaataa ctcgcaggtg agttgtaaag gttcagttga ttctacgatt
    21481 acggatttat tagaaaaagc attgaata
//
LOCUS       DAJTXY010000063        21497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTXY010000063 DAJTXY010000000
VERSION     DAJTXY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.063"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(28..2097)
                     /gene="glyS"
                     /locus_tag="OO566_002427"
     CDS             complement(28..2097)
                     /gene="glyS"
                     /locus_tag="OO566_002427"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6605679.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2107..3018)
                     /gene="glyQ"
                     /locus_tag="OO566_002428"
     CDS             complement(2107..3018)
                     /gene="glyQ"
                     /locus_tag="OO566_002428"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6605680.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3113..3412)
                     /locus_tag="OO566_002429"
     CDS             complement(3113..3412)
                     /locus_tag="OO566_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6605681.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3587..4582
                     /gene="wecH"
                     /locus_tag="OO566_002430"
     CDS             3587..4582
                     /gene="wecH"
                     /locus_tag="OO566_002430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6605682.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4624..5061)
                     /locus_tag="OO566_002431"
     CDS             complement(4624..5061)
                     /locus_tag="OO566_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6605683.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5107..5448)
                     /locus_tag="OO566_002432"
     CDS             complement(5107..5448)
                     /locus_tag="OO566_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605684.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5718..7070)
                     /gene="xylB"
                     /locus_tag="OO566_002433"
     CDS             complement(5718..7070)
                     /gene="xylB"
                     /locus_tag="OO566_002433"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6605685.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7142..8464)
                     /gene="xylA"
                     /locus_tag="OO566_002434"
                     /pseudo
     CDS             complement(7142..8464)
                     /gene="xylA"
                     /locus_tag="OO566_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8830..9822
                     /gene="xylF"
                     /locus_tag="OO566_002435"
     CDS             8830..9822
                     /gene="xylF"
                     /locus_tag="OO566_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6605686.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEREL"
     gene            9900..11441
                     /gene="xylG"
                     /locus_tag="OO566_002436"
     CDS             9900..11441
                     /gene="xylG"
                     /locus_tag="OO566_002436"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6605687.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11419..12600
                     /gene="xylH"
                     /locus_tag="OO566_002437"
     CDS             11419..12600
                     /gene="xylH"
                     /locus_tag="OO566_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6605688.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12678..13208
                     /locus_tag="OO566_002438"
     CDS             12678..13208
                     /locus_tag="OO566_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6605689.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13320..13856
                     /locus_tag="OO566_002439"
     CDS             13320..13856
                     /locus_tag="OO566_002439"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6605690.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGTRQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13964..14788)
                     /locus_tag="OO566_002440"
     CDS             complement(13964..14788)
                     /locus_tag="OO566_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6605691.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15108..17138
                     /gene="malS"
                     /locus_tag="OO566_002441"
                     /pseudo
     CDS             15108..17138
                     /gene="malS"
                     /locus_tag="OO566_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17316..18569
                     /gene="avtA"
                     /locus_tag="OO566_002442"
     CDS             17316..18569
                     /gene="avtA"
                     /locus_tag="OO566_002442"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6605692.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGETELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18720..19193)
                     /locus_tag="OO566_002443"
     CDS             complement(18720..19193)
                     /locus_tag="OO566_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6605693.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19295..20143)
                     /gene="yiaJ"
                     /locus_tag="OO566_002444"
     CDS             complement(19295..20143)
                     /gene="yiaJ"
                     /locus_tag="OO566_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6605694.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20344..21342
                     /gene="yiaK"
                     /locus_tag="OO566_002445"
     CDS             20344..21342
                     /gene="yiaK"
                     /locus_tag="OO566_002445"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6605695.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21354..>21497
                     /locus_tag="OO566_002446"
     CDS             21354..>21497
                     /locus_tag="OO566_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6605696.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5404 a         5483 c         5279 g         5331 t
ORIGIN      
        1 atggcaggct ttttaataac ggcgttatta ctgcaacagc gaaatatccg caacgcgcag
       61 gaacagttcg cgcagtttct ccagcatggt cagacggttg agacgcaatt ctttgtcatc
      121 aaccatgacc atcactttat cgaagaaagc atcaaccggc tcacgcagct ctgccagttc
      181 gaccagcgca tcctggtaac gaccttccgc aaagtacggc tccagcttgt cacgtagcac
      241 cacaacctgc atcgccagtt taatttcttc cggctctttc agggtagagg cattcacgcg
      301 atcgctcagc acttcgtcag atttcgccag gatgttagat acacgtttgt tcgccgccgc
      361 cagtgctgca gctgcttcca gggtacggaa gtgcgatacc gctttcatgc gggcatcgaa
      421 atcagccgga cgagtcggac gacgcgccag taccgcctgg atggtgtcaa cggtataacc
      481 ttcgtcctga taccaggcgc ggaagcgacc gagcataaag tcgataacat catcaactac
      541 gttggcatta gtcagcttat cgccatacag acgcaccgct tcttcggtca gcgtttgcag
      601 atcaaggttg aggttcttct caacgataat tcgcagcacg ccaagtgcgg cacgacgcag
      661 cgcaaacggg tctttgtcgc ctttcggatg ctgaccgata ccgaagatac ccgccagagt
      721 atccatctta tcagcaatcg ccagcgcaca agctaccggg ttggacggca ggtcatcacc
      781 ggcaaagcgc ggctgatact gctcattcag cgcgacggct acgtcttccg cttcgccatc
      841 gtgacgcgcg tagtgcatcc ccataacgcc ttgggtgtcg gtgaactcga agaccatgtt
      901 ggtcatcagg tcacacttag acagcaggcc cgcacgggtt gcgtggttaa cgtcagcgcc
      961 aatctgttcg gcgatccagc cagccagcgc ctggatgcgg tcagtcttgt cacgcagcgt
     1021 acccagctgt tgctggaaca gcacggtttg caggcgcggc aggttatctt ccagacgttt
     1081 tttgcggtcg gtgttgaaga agaactcggc atccgccaga cgtggacgaa cgactttctc
     1141 gttaccagag ataatttgct gcggatcttt cgattcgatg ttggcaacga agataaagtt
     1201 cggcagcagt ttgccgtcgt tcgcatacac cgggaagtac ttctggtcac ctttcatggt
     1261 gtaaaccagg gcttcagccg gcaccgcgag gaatttctct tcgaattttg cggtcagaac
     1321 gaccggccac tccaccagcg aagccacttc ttccagcagg ctttcgctta agtcagcgtt
     1381 accgccaatc ttacgcgctg cttcttcggc atcggcttta atcttcgctt tacgttcttc
     1441 gtaatcggcg atgactttcc cgcgctcacg cagaatttcc ggatactgat cggcgttgtc
     1501 gatggtgaat tccggttcgc ccataaagcg gtggccgcga atcacgcgat cggactgaat
     1561 gcccagaatg gttgccggaa tgactttgtc gcccagcagc agggtcacgg tgtgcaccgg
     1621 acgcacgaag tgcacgtcgc ttgcacccca gcgcatcagt ttcgggatcg gcagtttcgc
     1681 cagcgaagtc gcaaccatat tcggcagcag tgcttcggtg ctttcgccct tcacatgggc
     1741 gcgatacagc agccattcgc ctttatcggt agtcagacgc tcagcctggt caacggtaat
     1801 accacaacca cgcgcccaac cttctgccgc tttgctcggt tttccttcag cgtcgaacgc
     1861 ctgggcaatc gccgggccgc gtttttcgat ttcacgatct ggttgtgctt ccgccaggtt
     1921 agctactttc agcgccagac gacgcggagc agcaaaccat tgaacggtgc cgtgtgcgag
     1981 gcctgcgtta tccagctccg cagtaaagtt cgcagcaaag gactctgcca ggctgcgcag
     2041 tgcttttggt ggcagctctt cagtgccgat ttccaccaga aaagttttct cagacatagc
     2101 cgcctcttac ttatctttgt tgcacatcgg gaagccgagg gcttcacggg aagcgtaata
     2161 tgcttctgcc actgctttgg tcagggtgcg aatgcgcaga atgtagcgct gacgctcggt
     2221 gacggagatg gctttacgcg catccagcag gttgaagctg tgggcggctt tcagaatacg
     2281 ctcgtaggct ggcagcggca gcggattttc cagcgccagc agctgctgcg cttctttctc
     2341 gtactgctca aagcaggtga acaggaagtc cacatccgcg tattcgaagt tgtaagtgga
     2401 ctgctccact tcgttctgat ggaacacgtc gccgtaggtg gttttaccca gcgggccgtc
     2461 gctccagact aggtcgtaaa cgctgtctac gccctgaatg tacatggcca gacgttccag
     2521 accgtaggtg atctcgccgg taaccggttt acactccaga ccaccaacct gctggaagta
     2581 agtgaactgc gtcacttcca tgccgtttag ccacacttcc cagcccagtc cccaggcccc
     2641 cagagtcggg ttttcccagt tatcttccac gaaacggatg tcgtgaatag tcgggtccat
     2701 gcccagctct ttcagagaac cgaggtacag ctcctgaata ttgtccggcg atggcttaat
     2761 gaccacctgg aactgatagt agtgctgtaa acggttgggg ttttcgccgt agcgaccatc
     2821 ggtcgggcga cgagaaggct gaacataagc agccgccatc ggttctggcc ccagcgcgcg
     2881 cagacaggtc attgggtgag aggttcccgc gccgacttcc atgtccaatg gttgaacaat
     2941 ggtgcagccc tggcgagccc agtaatcctg taaggtcagg atcaagccct ggaaggtcct
     3001 ggtatcaaac ttttgcatat tatttcgtgc tggatacgtg tggatttaaa ggaagggatc
     3061 agtatacccg ctggatggaa gatatacagt acgaaacggg aaaaagcagg gcttaacgca
     3121 tggaaagatg caaaaaatgg ccatctgcgt caaaagaaca gacaaactgc tctttacgga
     3181 aggtataacc gcgcatttca tagcttccct ggaactgttc gaaggcggtg acgtcgattt
     3241 tttgctcgcc ggtgttataa cgttttgccg cctgatcctt gcataacgct tccatattca
     3301 acgaacgcag aggatcaacc tttacccgtt gtgctttctg cacgggtgac ggtgtagaac
     3361 aacccactag caccataagc gccattgccg gaaagaaagc gttcatcatc attaaattac
     3421 cgtctggttt gcaggtgagt cttattattt atatcggtat agtggctgat atcttacgaa
     3481 tttccttaag cctctatagg ctgtagtgat aaaactcaga ttattccagt gcgccaacca
     3541 aatgatttat cggtcagact ttttttaacc agatacagag acacagatgc agcccaaaat
     3601 ttactggatt gataacctgc gagggatagc gtgtttaatg gtggtgatga ttcacaccac
     3661 tacctggtat gtgaccaatg ctcatagtgt tagccccgtc acatgggata tcgccaatgt
     3721 tctgaattct gcctctcgtg tcagcgtgcc gctatttttc atgatttccg gctatctctt
     3781 ttttggcgaa cgcagcgccc agccgcgcca tttcttgcgt atcggcttat gtctgatttt
     3841 ttatagcgca atcgcactgc tctacattgc gctgtttacc tccatcaata tggagttagc
     3901 gctgaaaaac ctgctgcaaa agccagtgtt ttaccacttg tggtttttct tcgcgattgc
     3961 ggtgatttat ctggtttcac cgctgattca ggtgaagaac gtcggcggaa aaatgttgct
     4021 ggtgctaatg gtggtgattg gcattatcgc taacccaaac acagtgccgc agaaaattga
     4081 cggttttgaa tggctgccaa ttaacttata tatcaatggc gatacttttt actacattct
     4141 gtatggcatg ttgggccgcg ctatagggat gatggacaca cagcataaag cactgtcgtg
     4201 ggtgagcgcc gcgctgtttg cgacgggggt ttttattatc tctcgcggga cattatatga
     4261 attgcagtgg cgcggaaatt ttgccgatac ctggtatctt tactgtgggc cgatggtttt
     4321 tatctgcgca atcgcgctat tgactctggt taaaaacacg ctggatacgc gtaccattcg
     4381 cggacttggc ttaatctccc gccattcatt gggtatatac ggattccacg ccttgattat
     4441 ccatgcgctg cgcacccggg gaattgagct taaaaattgg ccaatactgg atattatttg
     4501 gatcttttgc gcgacgttgg cagcgagttt gttactttct atgctggtac aacgaatcga
     4561 cagaaacaga ttagtgagtt aagtaaaagc ccgttcacat tggactgacc gggcttacgt
     4621 gaattattcg ctgtattctt cctgggtaac ctgtgtttct tttggcgggt ttattccggc
     4681 atcacgaata ttcttctgca ccgctactgc accaaacaag cttaagaaga aagccagtcc
     4741 ataaaaacct ttttcactga gtaataatgt cgcattccac agacctacca tcagtagagc
     4801 aacagagata ataaagacag tcaggcaggt catataataa atggaagtgg tcggtatgcc
     4861 ttcatacttg tcccgaacgg tcttttgata agacgcagca gaaaacagtc ctaacaccag
     4921 tacggcaaaa taataccctt tttcatttaa ctgcatctct gcattccata gccctaacag
     4981 gtaggtaacg ataccaccaa cgagagctat ccatgacaca atactaaagg ccggtgaata
     5041 ggttgatatt ttgttgtcca tcacagtatt cctttcattc ctgaatgtgt aagagcttta
     5101 tgttgctcat accgagcgaa acaaacgcat ttgaccaaac aagcagacaa aaaaggcgat
     5161 ggggtagatc accatttcat aagcattgat aggtgcattt aaaacaccga taaacaagag
     5221 accgatagtg attaacgcaa ctaactggca gatatgggta aaaaaattat ccagttgccc
     5281 gagtttttct gcgcgaagat atgcatagtt accaaaagtt gctgtcatta acacgccaag
     5341 aaaatagcct tttccactta gcaatgggca tgagatccat agcccaacca gataaaccag
     5401 cgccccgaca acaaataata gttttgccac agtttttgag gttttcataa cgatctccat
     5461 atctaccagc gatacattac gagtaaccaa cgaaagacaa aactgaaaaa tgccattaac
     5521 aaatgatttt cagaataaat tcatactaaa tattaattaa ttagtgagat atatagatgt
     5581 gaattatccc ccacccggtc aggcagggga taacgtttac gccattaatg gcagaagttg
     5641 ctgatagagg cgacggaacg tttcacgtcg tggctgataa gcggcataac gctgcgcatc
     5701 tggtagatgc gactgttcta acggtagctg cggcaacaac tcaatgagcg atttctctgg
     5761 attcgccgca atctgcgcca gccttgctgc gcccagtgct ggccccacat cccccccgta
     5821 cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta ctcactacgc
     5881 gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg cacgacatcc
     5941 atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag ttcattgggg
     6001 ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt gtgtggtgta
     6061 cgctcgccgg aaagataagg cagaaaccaa actggctcgg cactttcatc agcctgttga
     6121 gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc ccaatccaga
     6181 cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag cgcatggcaa
     6241 aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc aaaatagacc
     6301 cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac accaactgca
     6361 ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc ccacgctttc
     6421 gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc gggcatctgg
     6481 tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt tgcgacatcc
     6541 agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt catacgcaga
     6601 cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc cggctcatgc
     6661 cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt gccggtaatc
     6721 actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca gcgcccgtcg
     6781 ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt tgctccatgc
     6841 atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc gcccagagct
     6901 ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga ccaaagtgga
     6961 tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa ccacctcacc ctgctcgttg
     7021 agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat atcgatcgtt
     7081 ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc acagttagcc
     7141 gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg cgaccactct
     7201 gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc agtgacattt
     7261 ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc tgcgcgatgc
     7321 gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc agtgccatcg
     7381 tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt tgacgacgta
     7441 ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga atttcataca
     7501 tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag cccagttgcg
     7561 catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg gtggctattt
     7621 catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc agtttaatct
     7681 ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg taatcatatt
     7741 gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctag aagccgattt
     7801 tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc tcctgacgca
     7861 agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca tagttttcac
     7921 cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc cagctgaaga
     7981 cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta aagcagttag
     8041 cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg acatcaacca
     8101 tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa acatccacat
     8161 cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg acatctgctt
     8221 tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta aacgccccca
     8281 caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg gcaaaacgca
     8341 agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga cggaatgcta
     8401 acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca aaataggctt
     8461 gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg taatattggg
     8521 cactcccttt caattgctca attatgttat ttcacactgc tattgagata attcacaagt
     8581 gtgcgctcgc tcgcaaaata aaatgggatg atgaaactgg gtaattcctc gaagataaaa
     8641 atgcaataag tacaattgcg caacaaaagt aagatctcgg tcataaatca agaaataaac
     8701 caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta gttgctaaaa
     8761 attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa ctacagaagg
     8821 ccctacacca tgaaaataaa gaacattcta ctcacccttt gcacctcact tctgcttacc
     8881 aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg cgattgatga tctccgtctt
     8941 gaacgctggc aaaaagatcg agatattttt gtgaaaaagg cagaatctct cggtgcgaaa
     9001 gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat tgaaaacatg
     9061 attaaccggg gtgtcgatgt tcttgtcatt attccgtata acggtcaggt attaagtaac
     9121 gttgtaaaag aagccaaaca agaaggtatt aaagtattag cttacgaccg tatgattaac
     9181 gatgcggata tcgattttta tatttctttc gataacgaaa aagtcggtga actgcaggca
     9241 aaagccttgg tcgatattgt tccgcaaggt aattacttcc tgatgggcgg ctcgccggta
     9301 gataacaacg ccaagctgtt ccgcgccgga caaatgaaag tgttaaaacc ttacgttgat
     9361 tctggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc ggaaaacgca
     9421 ttgaaaatta tggaaaacgc gctaaccgcc aataataaca aaattgatgc tgtagttgcc
     9481 tcaaacgatg ccaccgcagg tggggcaatt caggcattaa gcgcgcaagg tttatcaggg
     9541 aaagtagcaa tttccggcca ggatgcggat ctcgcaggta ttaaacgtat tgctgccggt
     9601 acgcaaacta tgacggtgta taaacctatt acattgctgg caaatactgc cgcagaaatt
     9661 gccgttgaat tgggcaatgg tcaggagcca aaagcagata cctcactgaa taatggcctg
     9721 aaagatgtcc cctcccgcct cctgacaccg atcgatgtga ataaaaacaa catcaaagat
     9781 acggtaatta aagacggatt ccacaaagag agagagctgt aagcgttacg ccccggcgcg
     9841 gagcgggggc gtgatttctc tccatgccgc gtgaatgaat tggcttaggt ggagtcgtta
     9901 tgccttatct acttgaaatg aagaacatta ccaaaacctt cggcagtgtg aaggcgattg
     9961 ataacgtcag cttgcggttg aatgctggcg aaatcgtctc actctgtggg gaaaatgggt
    10021 ctggtaaatc aacgctgatg aaagtgctgt gtggtattta tccccatggc tcctacgaag
    10081 gcgaaattat ttttgcggga gaagagattc aggcgagtca catccgcgat accgaacgca
    10141 aaggtatcgc catcattcac caggaattgg ccctggtgaa agaattgacc gtgctggaaa
    10201 atatcttcct gggtaacgaa ataacccaca atggcattat ggattatgac ctgatgacgc
    10261 tacgctatca gaagctgctc gcacaggtca gtttatccat ttcacctgat acccgcgttg
    10321 gcgatttagg gcttgggcaa caacaactgg ttgaaattgc caaggcactt aataaacagg
    10381 tgcgcttgtt aattctcgat gaaccgacag cctcattaac tgagcaggaa acgtcggttt
    10441 tactggatat tattcgcgat ctacaacagc acggtatcgc ctgtatttat atttcgcaca
    10501 aactcaacga agtcaaagcg atttccgata cgatttgcgt tattcgcgac ggacagcaca
    10561 ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat tatcaccatg atggtcgggc
    10621 gagagttaac cgcgctttac cctaatgaac cacataccac cggagatgaa atattacgta
    10681 ttgaacatct gacggcatgg catccggtca atcgtcatat taaacgagtt aatgatgtct
    10741 cgttttccct gaaacgtggc gaaatactgg gtattgccgg actcgttggt gccggacgta
    10801 ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca atgggaagga aaaatttata
    10861 ttgatggcaa acaggtagat attcgtaact gtcagcaagc catcgcccag gggattgcga
    10921 tggtacccga agacagaaag cgcgacggca tcgttccggt aatggcggtt ggtaaaaata
    10981 ttaccctcgc cgcactcaat aaatttaccg gtggcattag ccagcttgat gacgcggcag
    11041 agcaaaaatg tattctggaa tcaattcagc aactcaaagt taaaacgtcg tcccccgacc
    11101 ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc gatcctcgcc cgctgtctgt
    11161 tacttaaccc gcgcatcctc attcttgatg aacctaccag gggtattgat attggcgcga
    11221 aatatgagat ctacaaatta attaaccaac tcgtccagca gggtattgcc gttattgtca
    11281 tctcttccga attacctgaa gtgctcggcc ttagcgatcg tgtactggtg atgcatgaag
    11341 ggaaactaaa agccaacctg ataaatcata acctgactca ggagcaggtg atggaagccg
    11401 cattgaggag cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt ggccgtaccg
    11461 acatccggtg gcttttctgg gctgaaatca ctgaatttgc aggtcttcgt gatgattgca
    11521 gctatcatcg caatcatgct gttctttacc tggaccaccg atggtgccta cttaagcgcc
    11581 cgtaacgtct ccaacctgtt acgccagacc gcgattaccg gcatcctcgc ggtaggaatg
    11641 gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg gctcaatgat ggggctatta
    11701 ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc ctttgccact taccatcatt
    11761 gtgacgttgg ttctgggact gcttctcggt gcctggaacg gatggtgggt cgcgtaccgc
    11821 aaagtccctt catttattgt caccctcgcg ggcatgttgg catttcgcgg catactcatt
    11881 ggcatcacca acggcacgac tgtatccccc accagcgccg cgatgtcaca aattgggcaa
    11941 agctatctcc ccgccagtac cggcttcatc attggcgcgc ttggcttaat ggcttttgtt
    12001 ggttggcaat ggcgcggaag aatgcgccgt caggctttgg gtttacagtc tccggcctct
    12061 accgcagtag tcggtcgcca agctttaacc gctatcatcg tattaggcgc aatctggctg
    12121 ttgaatgatt accgtggcgt tcccactcct gttctgctgc tgacgttgct gttactcggc
    12181 ggaatgttta tggcaacgcg gacggcattt ggacgacgca tttatgccat cggcggcaat
    12241 ctggaagcag cacgtctctc cgggattaac gttgaacgca ccaaacttgc cgtgttcgcg
    12301 attaacggat taatggtagc catcgccgga ttaatcctta gttctagact tggcgctggt
    12361 tcaccttctg cgggaaatat cgccgaactg gacgcaattg cagcatgcgt gattggcggc
    12421 accagcctgg ctggcggtgt gggaagcgtt gccggagcag taatgggggc atttatcatg
    12481 gcttcactgg ataacggcat gagtatgatg gatgtaccga ccttctggca gtatatcgtt
    12541 aaaggtgcga ttctgttgct ggcagtatgg atggactccg caaccaaacg ccgttcttga
    12601 ttttgataaa aattttctca aagccggtta cgtattaccg gttttgagtt tttgcatgat
    12661 tcagcaggaa aagaaccatg tttactaaac gtcaccgcat cacattactg ttcaatgcca
    12721 ataaagccta tgaccggcag gtagtagaag gcgtagggga atatttacag gcatcacaat
    12781 cagaatggga tattttcatt gaagaagatt tccgcgcccg cattgataaa atcaaggact
    12841 ggttaggaga tgtcgtcatt gccgacttcg acgacaaaca gatcgagcaa gcgctggctg
    12901 atgtcgacgt ccccattgtt ggggttggcg gctcgtatca ccttgcagaa agttacccac
    12961 ccgttcatta cattgccacc gataactatg cgctggttga aagcgcattt ttgcatttaa
    13021 aagagaaagg cgttaaccgc tttgcttttt atggtcttcc ggaatcaagc ggcaaacgtt
    13081 gggccactga acgcgaatat gcatttcgtc agcttgtcgc cgaagaaaag tatcgcggag
    13141 tggtttatca ggggttagaa accgcgccag agaactggca acacgcgcaa aatcggctgg
    13201 cagactgact acaaacgctg ccaccgcaaa ccgggattat tgccgttact gacgcccgag
    13261 cgcggcatat tctgcaagta tgtgaacatc tacatattcc cgtaccggaa aaattatgcg
    13321 tgattggcat cgataacgaa gaactgaccc gctatctgtc gcgtgtcgcc ctttcttcgg
    13381 tcgctcaggg cacgcggcaa atgggctatc aggcggcaaa actgttgcat cgattattag
    13441 ataaagaaga aatgccgcta cagcgaattt tggtcccacc agttcgcgtc attgaacggc
    13501 gctcaacaga ttatcgctcg ctgaccgatc ccgccgttat tcaggccatg cattacattc
    13561 gtaatcacgc ctgtaaaggg attaaagtgg atcaggtact ggatgcggtc gggatctcgc
    13621 gctccaatct tgagaagcgt tttaaagaag aggtgggtga aaccatccat gcaatgattc
    13681 atgccgagaa gctggagaaa gcgcgcagtc tgctgatttc aaccaccttg tcgatcaatg
    13741 agatatcgca aatgtgcggt tatccatcgc tgcaatattt ctactctgtt tttaaaaaag
    13801 catatgacac aacgccaaaa gagtatcgcg atgtaaatag cgaggtcatg ttgtagcgat
    13861 gatgagaatt gtcggcgtca catcagggaa ctcagcacag ccacctgatg cgagcaaaag
    13921 ccttatccga ccgacaaatc agataaggct cgaggaaatg aaattacata tgcgccgcga
    13981 ttaaccgttg gttatcctgg tacattgcga acaggtagtt gttataactc ttccctttgg
    14041 tcgagtagcc cttcagcttg tgaatcatcg ctgtggcagt cacttcctga tccgctttac
    14101 gcagctgtgc acgcgattta cggaacgaag agtaagccgg gtgcgtattc aggttagtga
    14161 cataggcgct caccgattct ttgacagaac taaactgtga gtaccctttc actttacccg
    14221 gcgcattggt acaacgtcct ttcatgcatt tcatgccgaa cagattgtta ttgttgcgcg
    14281 ccagcttcga cgttccccaa ccgctttctg ctgcagccat cgtcgccacc atactggtag
    14341 ggataatgtc tacgcgttca agcaaggtat tccacgggat ttttcgcgta ttaccagacc
    14401 acttcacctt gtagcgtttg gcgatgtctt tcagacgcgc acgctcagca ggtgaccatt
    14461 ggccctgata ctgttttgaa atcagccagt tacgttctgc agtaatggct gcattttggc
    14521 tggtaatgta aggcattacg gtccggagaa acgccttttt ccttggtgtt ccggaagggt
    14581 attttcgcaa atcaggaagt gaactactct ttgcactatt gcgagaatac tcttgtttac
    14641 tgcttacctg tttattacta gctttagtta agtgggactt ttgactcgct gttgttgtgt
    14701 gcgtcttcgc taacacctca ctcgaaaaca ccagagtgag taacataaga atcatcgccc
    14761 catatcgtcg tatgggagtc aaaatcatca ggtctcctgg ccggatttaa tcattccaac
    14821 accttatatt tttcacaaat ttgagagttg aatctcaaat catatcaaaa atagctgtca
    14881 agagcacccc aaggaatagt ccaaatctga aactatgtca cgagttaacg attcagattg
    14941 gcgctaaatc gcagaaaatg tgggggttat cgcaaaattc agccgttttt tgcgcgcgat
    15001 cgctcaccct tgcttctcat cctgtggact taccgctcag ggatgagttt tgtctggctt
    15061 atcgctggca aactgtctga aatcgcagca ataaggactc atccaccatg aaactcgccg
    15121 cctgttttct gacactcctt cctggcttcg ccgttgccgc cagctggact tctccggggt
    15181 tccctgcctt tagcgaacag ggaacgggaa catttgtcag ccacgcgcag ttgcccaaag
    15241 gtacgcgtcc actcacgcta aattttgacc aacagtgctg gcagcctgca gatgcgataa
    15301 aactcaatca gatgctttcc ctgcaacctt gtagcaacac gccgcctcaa tggcgattgt
    15361 tcagggacgg caaatatacg ctgcaaatag acacccgctc cggtacgcca acattgatga
    15421 tttccatcca gaacgccgcc gaaccggtag caaacctggt ccgtgaatgc ccgaaatggg
    15481 atggattacc gctcacgctg gatgtcagcg ccactttccc ggaaggagcc gccgtacggg
    15541 attattacag ccaacaaatt gcgatagtga agaacggtca aataacgtta caacccgctg
    15601 ctaccagcaa cggtttactc ctgctggaac gggcagaaac tgacacatcc gcccctttcg
    15661 actggcataa cgccacggtt tactttgtgc tgacagatcg tttcgaaaac ggcgatccca
    15721 gtaatgacca gagttacgga cgtcataaag acggtatggc ggaaattggc acttttcacg
    15781 gcggcgattt acgcggcctg accaacaaac tggattacct ccagcagttg ggcgttaatg
    15841 ctttatggat aagcgcccca tttgagcaaa ttcacggctg ggtcggcggc ggtacaaaag
    15901 gcgatttccc gcattatgcc taccacggtt attacacaca ggactggacg aatcttgatg
    15961 ccaatatggg caacgaagcc gatctacgga cgctggttga tagcgcacat cagcgcggta
    16021 ttcgtattct ctttgatgtc gtgatgaacc acaccagcta tgccacgctg gcggatatgc
    16081 aggagtatca gtttggcgcg ttatatcttt ctggtgacga agtgaaaaaa acgctgggtg
    16141 aacgctggag cgactggaaa cctgccgccg ggcaaacctg gcatagcttt aacgattaca
    16201 ttaatttcag cgacaaaaca ggctgggata aatggtgggg aaaaaactgg atcagaacgg
    16261 atatcggcga ttacgacaat cctggattcg acgatctcac tatgtcgctg gcctttttgc
    16321 cggatatcaa aaccgaatca actaccgctt ctggtctgcc ggtgttctat aaaaacaaaa
    16381 cggatactca cgctaaagtc atcgaaggct ttacacctcg cgattactta acccactggt
    16441 taagtcagtg ggtccgcgac tatgggattg atggttttcg ggtcgatacc gccaaacatg
    16501 ttgagttgcc cgcctggcag caactgaaaa ccgaagccag ctccgcgctt cgcgaatgga
    16561 aaaaaactaa ccccgacaaa gcattagatg acaaaccttt ctggatgacc ggtgaagcct
    16621 agggccacgg cgtgatgcaa agtgactact atcgccacgg cttcgatgcg atgatcaatt
    16681 tcgattatca ggagcaggcg gcgaaagcag tcgattgtct ggcgcagatg gatacgacct
    16741 ggcagcaaat ggcggagaaa ttgcagggtt tcaacgtgtt gagctacctc tcgtcgcatg
    16801 atacccgtct gttccgtgaa gggggcgaca aagcagcaga gttattacta ttagcgccag
    16861 gcgcggtaca aatcttttat ggcgatgaat cctcgcgtcc gttcggtcct acaggttctg
    16921 atccgctgca aggtacacgt tcggatatga actggcagga tgttagcggt aaatctgccg
    16981 ccaacgtcgc gcactggcag aaaatcagcc agttccgcgc ccgccatccc gcaattggcg
    17041 cgggcaaaca aacgacactt tcgctgaagc agggctacgg ctttgttcgt gagcatggcg
    17101 acgataaagt gctggtcatc tgggctgggc aacagtgact tttccggctt cccgttcgtc
    17161 agtacctcgg gaagccgcca accaggataa aatgtcagcc ctaatcagcg ttgcgggata
    17221 aagcaccgct cactcttcaa cagaccgatt tgcaccccag caaatgtagc gttattgtta
    17281 ccttccttgc tacagagttc gacagatatc ccgctatgac attctccctt tttggtgaca
    17341 aatttacccg ccactccggc attacgctgc tgatggaaga tctgaacgac ggtttacgca
    17401 cgcctggcgc gattatgctc ggcggcggta atccggcgca gatcccggaa atgcaggact
    17461 acttccagac gctactgacc gacatgctgg aaagtggcaa agcgactgat gcactgtgta
    17521 actacgacgg tccacagggg gaaacggagc tactcacact gcttgccgga atgctgcgcg
    17581 agaagttggg ttgggatatc gaaccacaga atattgcact aacaaacggc agccagagcg
    17641 cgtttttcta cttatttaac ctgtttgccg gacgccgtgc cgatggtcgg gtcaaaaaag
    17701 tgctgttccc gctggcaccg gaatacattg gctacgctga cgccggactg gaagaagatc
    17761 tgtttgtctc tgcgcgtccg aatattgaac tgctgccgga aggccagttt aaataccacg
    17821 tcgattttga gcatctgcat attggcgaag aaaccgggat gatttgcgtc tcccggccga
    17881 cgaatccaac aggcaatgtg attactgacg aagagttgct gaagcttgac gcgctggcga
    17941 atcaacacgg cattccgctg gtgattgata acgcttatgg cgtcccgttc ccgggtatca
    18001 tcttcagtga agcgcgcccg ctatggaatc cgaatatcgt actgtgcatg agtctttcca
    18061 agctggggct gcctggctcc cgctgcggca ttatcatcgc caatgaaaaa atcatcactg
    18121 ccatcaccaa tatgaacggc attatcagcc tggcacctgg cggtattggt ccggcgatga
    18181 tgtgtgaaat gattaagcgt aacgatctgc tgcgcctgtc tgaaacagtc atcaaaccgt
    18241 tttactacca gcgtgttcag gaaactatcg ccatcattcg ccgctattta ccggaaaatc
    18301 gctgcctgat tcataaaccg gaaggagcca ttttcctctg gctatggttt aaggatttgc
    18361 ccattacgac cgagcagctc tatcagcgcc tgaaagcacg cggcgtgctg atggtgccgg
    18421 ggcacaactt cttcccggga ctggataaac cgtggccgca tacgcatcaa tgtatgcgca
    18481 tgaactacgt accagagccg gagaaaattg aggcgggggt gaagattctg gcggaagaga
    18541 tagaaagagc ttgggcagaa agtcactaaa aattgcctga tgcgctacgc ttatcaggcc
    18601 tacatgattt ctgcaatata ttgaatttgg aagaatttgt aggccggata aggcgtttac
    18661 gccgcatctg gcataaacaa agcgcactat tccggcgttg agaaacgccg gaaaacgttt
    18721 caaacaggct gctgctgtag ccgatgccgc tgcactttct cgacatccat gcactgcaac
    18781 gcctttgtcg ggcaggcttc aacacaggcc ggccccgtct cccgatgcca gcacagatcg
    18841 cacttaatcg ccctcgcctt tttgcgcgaa gaaacgacct ccatcgcacc aaacgggcaa
    18901 gccagcatac agcttttaca gccaatgcaa cgtgtttgtt caacgaaaat atgcccatgt
    18961 tcgcggctta tcgcgtcaac agggcagaca ttcgcgcacg gtgcatcttc acactgatga
    19021 caggctactg gcgtggtcca gctgtggtct ttaatgacac gaatacggga aataaactcg
    19081 tctggtgaca acgcagcgca atcctgattc tcctgatgcg acactgcgca agccacttca
    19141 caggtacggc aaccgataca tttcgtcgca tccgcaataa taaaccggtt catgatcatt
    19201 cctttgcact aaaacagcag agtgccagag agcgcgtccc tactgctttg atccaacgaa
    19261 ggaaatgtta aagttttggc acttgtccaa agcgttatgt gagcgcgccc aggtcatccc
    19321 tgacagtaaa tcccagttca ttagaaatag cctgcgcggt ttcacgcagt ggtttcagga
    19381 gatttttctc tcccacctgt ttcagacgtg atgtcgaaag cgaaatcgac acggcgtacg
    19441 gcacccgccc atgaatatca aacaccggaa cagcaataca ggagacgccg agttcgtttt
    19501 cttctctgtc catcgccgct ccgctttcac gaatgtgcgc cagttcgtcg aacatcgcgg
    19561 gcagctcggt aatggtattg tgggttaacg gctggatctc atgctgatgg ttttcccagt
    19621 atgacttcac gtagtccggg tgaccaaacg ccatgtagat cttgcccatt gcggaacagt
    19681 agagcggcat atgctggcca atataggcac gggttcgcag catcccggtt gtgggttcca
    19741 gcttataaat caaaatagcg tgatcgtctt cgcggctgga gaagttaatg gtttcaccag
    19801 tggcgatgtt cagtgcctca agatgcggag cggcgatatg aatgatattc agcgaagaca
    19861 gcgccttctg cccgacggca ataaatttgg tagtcaggcg gtaactccct gcggcgggcg
    19921 cggtggtcac atagccgcag gactgtaatc cctgcaataa gcgatggacg gtactcttat
    19981 ttaaaccagc cagctccgaa agatgcgcca acggacaacc gtttggatag ttgctcaaaa
    20041 tctcaatcag catcaacccg cgaaacagac tctggcttcc ggctggacgc tctttttcct
    20101 gcgccatctc gttctctttt tttcccatca cttctttccc cattttgtcg cgtcctgatg
    20161 gtagcgcaaa gtgtgccgta gttcacgatc tcgacagata atttataacc acttgatttt
    20221 tatgtctttt gaaattcatc aatcagattg ctttgttaaa aagtgatcga tatatttgaa
    20281 atctagtttc gcatattgaa attttaagac aaaaaagcga tcaaaaaaac aaggaagcct
    20341 aggatgaaag tgacatttga gcagttaaaa gcagccttta atcgggtctt aatttcacgc
    20401 ggcgttgaca gcgaaacggc tgacgcctgt gcagagatgt tcgcccgcac caccgaatcc
    20461 ggcgtttatt ctcacggcgt taatcgtttc cctcgtttca ttcaacaact ggaaaacggc
    20521 gatatcattc ctgatgccca acccaaacgt ataaccagcc tcggcgcaat tgaacagtgg
    20581 gatgcccagc gttcgatcgg caacctgacg gcgaaaaaga tgatggatcg cgccattgaa
    20641 ctggctgccg atcacggtat tggtctggtg gcacttcgta atgccaacca ctggatgcgc
    20701 ggcggcagct acggctggca ggcggcggaa aaaggctata ttggcatttg ctggaccaac
    20761 tccatcgccg taatgccgcc gtggggcgca aaagagtgtc gcatcggcac taacccgctg
    20821 atcgtcgcca ttccttccac gccgatcacc atggtcgata tgtcgatgtc gatgttctct
    20881 tacggcatgt tagaagttaa ccgcctggca ggccgtcagc tcccggtcga tggtggcttt
    20941 gatgatgagg gcaatttgac caaagaacct ggcgttatcg agaagaatcg ccgcattttg
    21001 ccgatgggct actggaaagg ttctggcatg tcgattgtgc tggatatgat cgctactctc
    21061 ctttccgacg gcgcatcggt tgccgaagtc acccaggaca acagcgacga atacggcatt
    21121 tcacaaattt ttattgccat tgaagtggac aagcttatcg acggtcccac ccgcgatgcc
    21181 aagctgcaac gtatcatgga ttacgttact accgctgaac gcgctgacga aaaccaggcc
    21241 atccgcttac ccggccacga atttactacc ctgctggccg aaaaccgccg taacggcatc
    21301 actgttgatg acagcgtgtg ggccaaaatc caggcgttat gaggagataa gtcatgattt
    21361 ttggacatat cgcgcagccc aatccgtgcc gtttgcccgc agccattgaa aaggcgctcg
    21421 attttctgcg cgctaccaac ttcaacgtcc tggagccggg cgttgtcgaa atcgacggca
    21481 agaatattta tgcgcaa
//
LOCUS       DAJTXY010000064        21241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTXY010000064 DAJTXY010000000
VERSION     DAJTXY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.064"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(34..1014)
                     /locus_tag="OO566_002447"
     CDS             complement(34..1014)
                     /locus_tag="OO566_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6605697.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1022..1732)
                     /locus_tag="OO566_002448"
     CDS             complement(1022..1732)
                     /locus_tag="OO566_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6605698.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1948..3771)
                     /gene="typA"
                     /locus_tag="OO566_002449"
     CDS             complement(1948..3771)
                     /gene="typA"
                     /locus_tag="OO566_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6605699.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4144..5553
                     /gene="glnA"
                     /locus_tag="OO566_002450"
     CDS             4144..5553
                     /gene="glnA"
                     /locus_tag="OO566_002450"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6605700.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5726..6775
                     /gene="glnL"
                     /locus_tag="OO566_002451"
     CDS             5726..6775
                     /gene="glnL"
                     /locus_tag="OO566_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6605701.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6787..8196
                     /gene="glnG"
                     /locus_tag="OO566_002452"
     CDS             6787..8196
                     /gene="glnG"
                     /locus_tag="OO566_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6605702.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8308..8418
                     /locus_tag="OO566_002453"
     CDS             8308..8418
                     /locus_tag="OO566_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6605703.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8647..10020)
                     /gene="hemN"
                     /locus_tag="OO566_002454"
     CDS             complement(8647..10020)
                     /gene="hemN"
                     /locus_tag="OO566_002454"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6605704.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10209..10718)
                     /gene="yihI"
                     /locus_tag="OO566_002455"
     CDS             complement(10209..10718)
                     /gene="yihI"
                     /locus_tag="OO566_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6605705.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11300..11932
                     /locus_tag="OO566_002456"
     CDS             11300..11932
                     /locus_tag="OO566_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6605706.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12314..15100)
                     /gene="polA"
                     /locus_tag="OO566_002457"
     CDS             complement(12314..15100)
                     /gene="polA"
                     /locus_tag="OO566_002457"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6605707.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYTEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15464..16396
                     /locus_tag="OO566_002458"
     CDS             15464..16396
                     /locus_tag="OO566_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6605708.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16437..17867)
                     /locus_tag="OO566_002459"
                     /pseudo
     CDS             complement(16437..17867)
                     /locus_tag="OO566_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
     gene            complement(18022..18648)
                     /gene="dsbA"
                     /locus_tag="OO566_002460"
     CDS             complement(18022..18648)
                     /gene="dsbA"
                     /locus_tag="OO566_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6605709.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18665..19651)
                     /gene="srkA"
                     /locus_tag="OO566_002461"
     CDS             complement(18665..19651)
                     /gene="srkA"
                     /locus_tag="OO566_002461"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6605710.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRQTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19728..19997)
                     /locus_tag="OO566_002462"
     CDS             complement(19728..19997)
                     /locus_tag="OO566_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6605711.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20067..20651
                     /gene="mobA"
                     /locus_tag="OO566_002463"
     CDS             20067..20651
                     /gene="mobA"
                     /locus_tag="OO566_002463"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6605712.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20633..21160
                     /gene="mobB"
                     /locus_tag="OO566_002464"
     CDS             20633..21160
                     /gene="mobB"
                     /locus_tag="OO566_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6605713.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5178 a         5346 c         5121 g         5596 t
ORIGIN      
        1 tggttttatt aactgcgtat gagttaaaac ttattagtgg ttcagaagat ggtttgcctc
       61 ttgtttgatt tgttgtagta cgttacgcac atgatttatc tgattaattg catcttcttt
      121 ttctttacgt aaccgcgcat ccagtaaatg attttctggt aggggtgttt cactcatctg
      181 gcgataaggg atccacggat tatcatcttc gtcttcaaag cggaacgccg gcgcatagta
      241 atcaacctct tcttccaggc catatgccgt cacctgcggc tcatcatcac caaggcagat
      301 aaggtgcgtt aataacgctt tgaatggcat atcaccctga cctgaaatac aggctttatg
      361 acccaggcca ccctgttctt taacgatcaa ggcatcttta atatggacct gggtaatatg
      421 cggtgccatc gtttttaagg cgtcgatggg atgctcattt gcattgatca tgttagcaaa
      481 atcaaacagt aaggaaagag actccatctc actttctttg accagcgaca ccagctcatg
      541 actttttaaa tcttcatgct gctcaatcgt aaaagtcagg ccgctgtcct gatacgtttc
      601 ccgtacatag gcaatgtcgt tagcgataat cgataatacg tcgcgcaaat taccttcata
      661 acgtggataa aaacgtacgg acgttgcccc agttttcaac gcgatggcga cggcttcgtc
      721 aatagatgcc ttatctgaag cgctggtttc aatatgaata tcaaggttca gacggcgggc
      781 tttatcccca aaggcagaga gttctttatc gtccatatta ccaagagaaa aacgctcgcc
      841 atccagaaca tggattttga cgccacgtag attattttca cttgcgatat caagtaaatc
      901 agcaggcaac actctttcca gccgcatatt taaatgaaag gcataagcat gtaaataaag
      961 aggaagagtg tcgacacgtt gtagaatctt tcttgcgtta tttattgtca ccataaaaca
     1021 cctacagatt aatgatgagg tctacggcct caaatcgcca atattcaata tctatctgga
     1081 caatattgcc ttgctggtcg gcgcgatgct tttcaattaa gatcgctggc atacccgcag
     1141 aaccgccaag atacttgctg atatcgcccg gcattctgac gggtttgaaa atcaattttt
     1201 ttactaccgt ctctttttgg tagcactttt cccagactgc agaaaatgag tggttttcaa
     1261 gttgttcaat aaaacccgga gcaacttcag gattaatata tgtttcgtga tagaaaactt
     1321 tatgtccttc cagcgccccc cagccggtga tgcgatagag ttcagttgat ggcgtcactg
     1381 caatcagtgg cgcgagcgtc tcgggaatat cgctggtacg gtttttctcg gtaaaccccc
     1441 aagaaggctc tcgcccttgc tcaattgcgg cccgctgaaa gctcgccgac agctccggac
     1501 tgtaattaaa tcgcggctgg gtcacgaacc accccttacg atccttacgg aagattttgg
     1561 attctgcctc gaggttcaac aacgcctggc gcagcgtcat acgtttaatg cccagcagtt
     1621 ctcccagctc tctttctgaa gggagttttc cacccggcgt ggtgatgcca tctttcaacc
     1681 accgatccag tttctctttt gcattttcta cggtggattg attctccgcc attgtttaat
     1741 ccttttggtt taaaccagat gaagcatctt acgcttacgc caaaaaagaa agcaattaac
     1801 ggtgtatgac tttgcagata attgtgacaa agctctctat tgacgtaatc acagacttat
     1861 aagggagata ctcgcattgg tgtgcgctca cgacaggtaa aaaaaacctg ccatcgctgg
     1921 caggtttttt atgactaaaa aacgaaatta atcgtctttc ggtgcgcggt tggcgcggcg
     1981 acgatcgttt tccgtcaggt gacgtttacg aatacggata gaggtcggag tcacttctac
     2041 cagttcgtca tcatcgatga actccagagc ttgttccaga gtcatgcgga taggcggaac
     2101 cagaacaaca gcttcgtcag taccggaagc acgcatgttg gtcagtttct taccggtcag
     2161 gcagtttaca gtcaggtcgt tagagcggct atgaataccg ataatctgac cttcgtaaac
     2221 ttctgcaccg tgaccgagga acagcttacc gcgatcctgc agaccgaaca gcgcgaacgc
     2281 gacagcttta ccctgaccgt tagagatcag tacaccgttc tgacgctgac ccacttcgcc
     2341 cggacgtacg tcgtcgtagt ggctgaaggt ggagtacagc agaccagtac cggaagtcat
     2401 ggtcatgaac tcagaacgga agccaatcag accacggctt gggatcacgt agtcgagacg
     2461 tacgcggcct ttaccgtctg gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc
     2521 ctgcatcaca gaaccctgat gctgttcttc aacgtccagc gtcacgtttt catacggctc
     2581 ttgtttacga ccgtcgattt cacggaagat tactttcgga cgggataccg ccagttcgaa
     2641 accttcacga cgcatgtttt cgataagaac agagaggtgc agttcaccac gaccagaaac
     2701 gcggaacgca tcggcgtctt cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc
     2761 tttgttcaga cgatcgagga tctgacgaga cgttacgaac ttaccttctt taccgcagaa
     2821 cggcgaggtg ttaacgcaga agaacataga aacggtcggc tcatcaacgg agagtgccgg
     2881 cagcgcttca acgttttgcg tgtcgcaaac ggtgtcagaa atgttcagtt cgcccaggcc
     2941 cgtgatcgcc acgatatcgc cagcttccgc cagatcggtt tcgatacgtt ccagaccgag
     3001 gtggcccagc actttaccga ctttcgcgtt acgggttttg ccttcgctat cgatgatagt
     3061 aacctgctgg ttcggcttca ctttaccacg cttgatgcgg ccaatgccga taacgccaac
     3121 atagctgttg taatcgagct gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc
     3181 cggcgcagga acgtggtcaa caatcgcctg atacagcggg gtcatgtctt ccgccatatc
     3241 ttcgtggtcc agacccgcga taccgttcag cgcagaagcg taaacgatcg ggaagtccag
     3301 ctgctcgtcg gtcgcgtcga ggttaacgaa cagatcgaat acctgatcca caacccaatc
     3361 aggacgcgcg ccagggcggt caactttgtt gataacaaca atcggcttca ggccgtaagc
     3421 aaacgctttt ttggttacga agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac
     3481 cagcagcact gagtctacca tggacattac acgttcaact tcaccaccga agtcggcgtg
     3541 ccccggggta tcaacgatgt tgatacggta atcattccat ttgatagcgg tgtttttcgc
     3601 gaggatggta atcccacgct ctttctccaa atcgttggag tccatcacgc gctcttgggt
     3661 ttcggcacga gagtcgaacg taccggattg ttggagcagc ttgtctacca aggtggtttt
     3721 accatggtct acgtgcgcga tgatggcgat attacgcaat ttttcgatca caactttgcc
     3781 tcaggcatta gaaatagcgc gttattgtac acggattaat cgcactacaa aacaggatca
     3841 caaacatcct ccgcaaacaa gtattgcaga gtccctttgt gatcgctttc acggagcata
     3901 aaaagggtta tccaaaggtc attgcaccaa tatggtgctt aatgtttcca ttgaagcact
     3961 atattggtgc aacattcaca tcatggtgca gcccttttgc acgatgttaa gcatgataac
     4021 gccttttagg ggcaatttaa aagttggcac agatttcgct ttatcttttt tacggcgaca
     4081 cggccaaaat aattgcagat ttcgttacca cgacgacaat gaccaatcca ggagagttaa
     4141 agtatgtccg ctgaacacgt actgacgatg ctgaacgagc acgaagtgaa gtttgttgat
     4201 ttgcgcttca ccgataccaa aggtaaagaa caacacgtca ctatccctgc tcatcaggtg
     4261 aatgctgaat tcttcgaaga aggcaaaatg tttgacggct cctcgattgg cggctggaaa
     4321 ggcattaacg aatccgacat ggtgctgatg ccagacgcat ccaccgcagt gattgacccg
     4381 ttcttcgccg actccaccct gattatccgt tgcgacatcc ttgaacctgg caccctgcaa
     4441 ggctatgacc gtgacccgcg ctctattgcg aagcgcgccg aagattacct gcgttccact
     4501 ggcattgccg acaccgtact gttcgggcca gaacctgaat tcttcctgtt cgatgacatc
     4561 cgtttcggat catctatctc cggttcccac gttgctatcg acgatatcga aggcgcatgg
     4621 aactcctcca cccaatacga aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt
     4681 tacttcccgg ttccgccggt agactctgct caggatattc gttctgaaat gtgtctggtg
     4741 atggaacaga tgggtctggt ggttgaagcc catcaccacg aagtagcgac tgctggtcag
     4801 aacgaagtgg ctacccgctt caataccatg accaaaaaag ctgacgaaat tcagatctac
     4861 aaatatgtcg tgcataacgt agcgcaccgc ttcggtaaaa ccgcgacctt tatgccaaaa
     4921 ccgatgttcg gtgataacgg ctccggtatg cactgccaca tgtctctgtc taaaaacggc
     4981 gttaacctgt tcgcaggcga caaatacgca ggtctgtctg agcaggcgct gtactacatt
     5041 ggcggcgtaa tcaaacacgc taaagcgatt aacgccctgg caaacccgac caccaactct
     5101 tataagcgtc tggtcccggg ctacgaagca ccggtaatgc tggcttactc tgcgcgtaac
     5161 cgttctgcgt ctatccgtat tccggtggtt tcttctccga aagcacgtcg tatcgaagta
     5221 cgtttcccgg atccggcagc taacccgtac ctgtgctttg ctgccctgct gatggccggt
     5281 cttgatggta tcaagaacaa gatccatccg ggcgaagcca tggacaaaaa cctgtatgac
     5341 ctgccgccag aagaagcgaa agagatccca caggttgcag gctctctgga agaagcactg
     5401 aacgaactgg atctggaccg cgagttcctg aaagccggtg gcgtgttcac ggatgaagcg
     5461 atcgatgcgt acatcgctct gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat
     5521 ccggtagagt ttgagctgta ctacagcgtc taatagttga agttgtacta cccggcgcac
     5581 aacgccggga tttagttgcc gtggaaactt tcagcccatc tctgcatggg cttttttctc
     5641 cgtcaattct ctgatgcttc gcgcttttta tccgtaaaaa gctataatgc actaaaatgg
     5701 tgcaacctgt tcaggagact gctttatggc aacaggcacg cagcccgatg ctgggcagat
     5761 cctcaactcg ctgattaaca gtattttgtt aattgatgac aatctggcga tccattacgc
     5821 caaccccgcc gcgcaacaac tgctcgccca aagctcccgc aaattgtttg gtacgccgtt
     5881 accggaactg ttgagctact tctcattaaa tatcgagctg atgcaagaaa gtctggaggc
     5941 ggggcaaggc tttaccgata acgaagtgac gctggtcatc gatggtcgct cgcatatcct
     6001 ttctgtgacg gcccagcgta tgccggacgg catgatcctg ctggagatgg caccgatgga
     6061 taaccagcgc cgcttaagcc aggaacaact acaacatgct caacaagtag ctgcccgtga
     6121 tttagtgcgc ggcctggcac atgagattaa aaatccgctt ggcggtttac gtggcgcggc
     6181 gcagttactc agcaaagcgt tacctgaccc atcactactc gaatatacca aagtgattat
     6241 cgaacaggcg gaccggctgc gaaatttggt cgaccgtctg ttggggccgc agctgcccgg
     6301 tacgcgcgtt accgaaagta ttcacaaagt ggctgaacgc gtggtaacgc tggtgtcgat
     6361 ggaactgccg gacaacgtgc ggttgattcg tgattacgat cccagcctgc cggaactggc
     6421 gcacgacccg gatcaaattg aacaggtctt gctgaatatt gtgcgcaatg cgctacaggc
     6481 gctggggccg gaaggcggtg aaatcattct gcgtacccgc accgcgtttc aactgacctt
     6541 acacggcgag cgctaccggc tggcggcgcg gattgatgtg gaagataacg ggccgggcat
     6601 tccgcctcat ttgcaggata cgctgtttta cccgatggtc agcggccgcg aaggtggcac
     6661 cgggcttggc ttatccatcg cccgtaattt gattgatcag cattcaggca aaattgaatt
     6721 taccagttgg ccaggtcata ccgagttctc ggtttacctg cctatcagga aataaaggtg
     6781 acgtttatgc aacgagggat agtctgggta gtcgatgacg atagttccat ccgttgggtg
     6841 cttgaacgtg cgctcgctgg agcgggttta acctgtacga catttgagaa cggcgcggaa
     6901 gtactggagg cgctggcgag caaaacgccg gatgtgctgc tttcagatat ccgtatgccg
     6961 ggaatggacg ggctggcgct gctcaagcag attaaacagc gccatccaat gcttccggtc
     7021 atcattatga ccgcacattc cgatctggat gctgccgtca gcgcctatca acaaggggcg
     7081 tttgattatc tgcccaaacc gtttgatatc gacgaagcag tggcgctggt tgagcgcgct
     7141 atcagtcatt accaggaaca gcagcagccg cgtaatattc agcttaacgg cccaacgacc
     7201 gatatcatcg gcgaagcgca agccatgcag gacgtgttcc gtattatcgg tcggctttcg
     7261 cgttcttcta ttagcgtgct gattaacggc gaatccggca ccggtaaaga actggtcgct
     7321 catgccctgc atcgccacag tccgcgcacc aaagcgccgt ttatcgcgct gaatatggca
     7381 gctatcccaa aagatttgat cgaatcagaa ctgtttggcc acgagaaagg cgcgtttact
     7441 ggcgcgaata ccattcgtca ggggcgtttt gaacaggccg atggcggcac gttatttctc
     7501 gacgaaattg gagatatgcc gctggatgtg cagacgcgtt tgctgcgcgt gctggcagac
     7561 ggtcagtttt atcgcgttgg cggctatgcg ccggtgaaag tggatgtgcg gattatcgct
     7621 gccactcacc agaatcttga acagcgggtg caggaaggta agttccgtga ggatctgttc
     7681 caccgcctga acgttatccg cgttcatctg ccgccgctgc gcgaacgtcg ggaagatatt
     7741 ccccgtctgg cgcgccattt tttacaggtt gccgcgcgcg aactgggcgt agaagcgaag
     7801 ttgctgcatc cggaaaccga agctgctctg acgcgtctgg cgtggccagg caacgtgcgc
     7861 cagctggaaa acacctgccg ctggctaacg gtgatggccg ccgggcagga agtgttgatt
     7921 caggatttgc ccggcgaact gtttgaatca acggttgcgg agagtacttc gcaaatgcaa
     7981 ccggacagtt gggcgacact tttagcgcag tgggcagaca gagcgctgcg ttccggtcat
     8041 caaaatctgc tttccgaagc gcagccagag ctggagcgga cgttactgac gaccgcgttg
     8101 cgacatacgc aggggcataa acaggaagcg gcgcggctac tcggctgggg ccgcaacact
     8161 ctgacgcgta agttaaaaga gctggggatg gagtgattca cggcttgtgt gtaaagattg
     8221 attattgagc gcaaattgct ggtattttgc gctttactgt tccgataagt tcagtatgat
     8281 cttgcccgga aaacggggag agtcattatg ctggaatcaa taattaatct ggtatcgagt
     8341 ggcgcagttg acagccacac accgcaaact gccgttgctg ccgtgctgtg tgccgcgatg
     8401 attgggctgt ttagctgaag ctcgttgttt atgccggatg cggagaaagt cgtaggcctg
     8461 ataagacgcg accagcgtcg catcaggcat catgctcaga tgcctgatgc ggcgtaaacg
     8521 ccttatccgg cctacaaaag caataatttc aacaggtttt gttgcccagc gcatcaggca
     8581 atcttgttct cagcacattg atggcgcttt cgttttctac tttgtaaacg aagcgccatt
     8641 cactctttaa atcacccgtg agaactgctg catccgcgct ttctggcgca gataggtatc
     8701 aaagcacatg caaatgttgc ggatcagcaa gcgacctttc gccgtcacct gtattccctt
     8761 ctcatccaca tccaccagcc catcttttgc taacggggcg agcagcttga gatcttccgc
     8821 aaagtaatca gcgaagtgca aatcccactg tttctcaaga ggggcgtaat ccagacggaa
     8881 gttgcagatg agcgacttaa tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat
     8941 accacgccac agcgcattgc cttgttcatc cacttgctga tagtactgct tcaactcttt
     9001 ctggttctgc gcgtagcagt cgccaatcat gctgatggcg gaaacgccca tccccagcag
     9061 atcggtatcg ccctgagtgg tgtagccctg gaagttacga tgcagcacgc cttcacgctg
     9121 ggccaccgcc agctcgtcat caggacgggc aaagtgatcc ataccgataa actgatagcc
     9181 cgattgcgtc aggaaggcga tggtttcctg caggatatcg agtttttgct gcggactcgg
     9241 caggtcagca tctttgattt tgcgctgagc agcaaaaatg gtcggcagat gcgcgtagtt
     9301 aaaaacgctc agacgatcgg ggttcagctc cgccacacgt ttcagggtaa aggcgaaact
     9361 ctccggcgtc tgtttcggca ggccgtaaat caggtcgatg ttggtggagg taaaaccaat
     9421 ctcacgcgca tggttaagca gtgcaaagat gaactcttca tcctgctcgc ggttaaccag
     9481 acgttgcact tctttgttga agtcctgcac gcccatgctc aggcgattaa agccttcggc
     9541 gcgtaaatga tcgagtacat ccagttcgat ttcccgcgga tcgacttcga tcgaaatctc
     9601 cgcatcggta ttgaaatgga agttttcgcg cagcagcttc atcagacggc tgatttgcgc
     9661 tttattcaga tacgtcggcg ttccgccgcc ccagtgcaat tggctgacgt gacgcccggc
     9721 gaacagcggt gcacgatgga cgatttcttg ctccagcgcg tccagatact gatcggcctt
     9781 gtgctgctgg cgagtaacaa tcttattgca accgcagaag taacaaagct tatggcagaa
     9841 cgggatatgt acgtagagag ataatggacg ctcaggatag cgcgccacgg cttgtaaaaa
     9901 cgcctgttcg ccgaagtctt ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg
     9961 cccggaatag ttatatttct ggatcagggc cagatcccag tcgatttgct gtacagacat
    10021 gctcactcct tccgatggcg tctctggcgg ctacggcgta ccggcggatg cccgtttcgg
    10081 gtcatcagcc ggttgcgcag ccacttttgt cgccgcgaca accgctgtag tttaacgaat
    10141 aaccacacca gataacatat aaccggaagg gttatcagca gaacgggaag gcccacggcg
    10201 gcaatccgtt agttgccccg cagcagacgc atcatgtctt cttgcttctc gtcttcttcc
    10261 tcttcttcgt catcgtcata agagaggccg agtttctgca tcaactcatc aatacgatcc
    10321 agtttagcat ccacccagga ttgctcttcg gcgctcaggg tttcgcctgc ttccagacgt
    10381 tccagcagcg catccagacg ctcatccgtt tccagtaatt ccaactccgc ctgcggtgaa
    10441 agcataggtt tctcactctt cggtttgtgc tgtttggtga ctttttcagt cactcccaat
    10501 ggaatagggg ttttactgcc aatacgtgga tcttttggtg cgttctggcc tttgctgcct
    10561 gacgtggtgt ttccgcccgc tgcgcggctg cccggcgcat gaccacgacg ttttttctga
    10621 cgcttacggt cacgagcttc ctgatccagc tcctcgcgtg tttttcgacg cgcttttgca
    10681 tgacctttgc tgcgtgagtt tgaagatgat ggtttcataa tggttcgtct tttagccgtt
    10741 ttattcagta tagatttgcg gcggaatcta acagaaagca agcaaagaaa aaaggcgaca
    10801 gattactctg tcgccttttt tcctgactca taacccttaa cgggtcttac aatccttgca
    10861 ggccgccaac acaccctgtt tttccattag ccggaacaat ccgtgttgat tccatttccg
    10921 tttaattacg tctccggacg tttgtcttcc tgacaatcct ctgtcttcgc ctcctggcgc
    10981 tcctgaccct catcctgagt cattgttcct gttagcgtcc tcgctctatg ggtgctactt
    11041 tacgcctttg ctttaaacaa acaatcaacc gccatcagta tttgcacatt ttcagagttt
    11101 tatataaaag caattcactg atttatatag cttatttgac atgcatcgca gaattttctc
    11161 tgctatttcg cataaagact atcggcaatg tctcacaaag cgtatggctt ttacttacaa
    11221 cgctcacgcc gaaaaatccg ccttttatta tggttcaggt ataatcgcca caaagctttg
    11281 actgacggag acgaccgctt tgactaattt gaattatcaa cagacgcatt ttgtgatgag
    11341 tgcgcctgat attcgccacc taccttccga taccggaatt gaagtggctt ttgcaggccg
    11401 ttccaacgca ggtaaatcca gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg
    11461 tacctcaaaa accccagggc gcacccagct tatcaacctg tttgaagtgg ctgacggcaa
    11521 gcgtctggtt gacttgcctg ggtacggtta tgcggaagtc ccggaagaga tgaagcgcaa
    11581 atggcagcgt gcgctcggcg aatacctcga aaaacgtcag agcctgcaag gtctggtggt
    11641 gctaatggat attcgccatc cgctgaaaga tttggatcag cagatgattg agtgggcggt
    11701 agacagcaat atcgccgttc tggtgctgct gaccaaagcg gacaaactgg caagcggcgc
    11761 acgtaaagcg ctattgaata tggtgcgtga agctgtactg gcgtttaacg gtgatgtgca
    11821 ggttgaaacg ttttcttcgt tgaagaaaca aggcgtagac aagctgcggc agaaactgga
    11881 tacctggttt agcgagatgc agcctgtaga agaaacgcag gacggcgaat aattttcttg
    11941 ccttaatgct tgtgccggat gtggcgtatc cggcccgtaa attcaataga ttacaattct
    12001 accgttggcc tgataaacat agcgcatcag gcattacgga tcttttcttt cgcccaataa
    12061 aaaacgcccc agtcattact gactggggcg gctaaaatat tcagccaaat ccgattacgt
    12121 gaagtaaaag gtctgaaaga tagaacatct tacctctgta ccctacgcga ctaactttac
    12181 tcttttttgt tcagttcaga aagcactttt tgtaattttt tttcagttgt tgcataggaa
    12241 tttctaatag ccatcacaaa acgtgacagc ttatgttgct tacttacgaa aaaagggcat
    12301 gttcaggcga atcttagtgc gcctgatccc agttttcgcc actccccact tccaccagca
    12361 acggcacatc cagacgggta cagttttcca tcagttgatg aatctgcttc gcgacggcat
    12421 caacatcatc tttatgaact tcaaatacca gttcatcgtg tacctgcatg atcatacgta
    12481 cacgcggttg ctcagcctgt aaccacgcat caacggcaat catcgcccgt ttgataatgt
    12541 cggcggcggt tccctgcatt ggcgcgttaa tggctgcacg ttcagccgct gcacgacgag
    12601 caccattgct ggatttgata tccggcagat acagacggcg tccgtccagc gtttcaacgt
    12661 agcccagctc tttcgcctga gcacgggtgc gttccatata ctccagcacg ccagggtagc
    12721 gttcgaagta aaggtccatg tacttctgcg cttctttacg tggaatgttc aattgccgcg
    12781 ccagaccgaa agcactcatg ccataaatca gaccaaagtt gatcgctttc gcgctacggc
    12841 gttgctcgct ggtgacggtt tccagtggca aaccaaacac ttctgccgcc gttgcccggt
    12901 ggatatcttt tccttccgcg aatgcggtca gcaagccttt atcacgcgag agatgcgcca
    12961 taatgcgcag ttcaatctgc gagtagtccg ctgagacaat cacataatcc tctggcgcaa
    13021 taaacgcctg gcggatacga cgaccttctt cgttacgcac cggaatgttt tgcaggttag
    13081 gatcggttga cgataaacgt cccgttgcag ttactgcctg gtgataagag gtatgcacac
    13141 gcccggtttt cgggttgatc atcagcggca gcttgtcggt gtaagtcgac ttcagcttcg
    13201 ccagaccacg atactccaga atcacttttg gcaacggata gtccagcgcc agttcttcca
    13261 gtacctcttc cgacgttgac ggcgcgccac ccggcgtttt cttcagcggt ttaataccct
    13321 gtttttcaaa cagaatggtt tgcaactgct tggtggaaga aaggttaaat tcttcacctg
    13381 caatttcatg cgctttcttt tccagctcag ccagacgcaa ggtgagctct tcagaatgat
    13441 tgtgcagcac tttcggatcg atcttcacac cgttacgttc aatgcgtgaa agcaccggca
    13501 ccagcggcat ttcgatattc tcgaagacgt tcaacggccc tttgtgtttt tgcagatccg
    13561 gccacatttt cagatgcaac tgcaaggtga catctgcatc ttcggcggcg taacgtccgg
    13621 cctcttcgag ggcaatctgg ttaaaggtca gttgattttt gcctttaccc gcaatctctt
    13681 caaaagtgat ggttttgtgc ttcaaccaac gttccgcgag gctgtccata tcgtgacgcc
    13741 cggcaacgct attgagaatg taggactcca gcatggtatc aaacgcaatc ccacgcagtt
    13801 caatgccgta attcgccaga ataccgcgat cgtatttcag gttttgcccg accttcagcg
    13861 ccttttcatc ttccagcagc ggttttagca actcgagtgc acgctcgtga gagatttgat
    13921 cgggcgcatc aagataatca tgagcaaccg gaatatatgc cgctacgcct ggctcgatag
    13981 caaaagaaag cccgaccagg ttagcagaga tgttatcaag gctgtcggtt tcggtatcaa
    14041 atgcaaatac cggcgctttt tccagcttcg caatccacgc tttcagtgtt tcttcatcaa
    14101 ggatagtgac gtagttgtca taagaaatca ccgttgccgt cacttctggc gcttcgtctg
    14161 caacactggt ttcctgcggc ttcgcggctg gttttgcccc tttggcctgt aaccatttgc
    14221 ccgcttcgac atcagcagtc cagcgtttga actcatactt tttgaacagc cccaacaact
    14281 cttccgctgc cggttgctgc acttccagtt gttcacaggt cagctccagt tcaacatcgg
    14341 ttttaatcgt cgccaactgg tatgagagat aagcaacttc tttgttttgc tcgagcttcg
    14401 ctgccattgt tttcgcgcca cggaagctca acccagcaat tttttctggc tcggtataca
    14461 gcgtatccag cccgccaaga ccttgcagca atgcctgcgc ggttttttca ccgacgcccg
    14521 gtacgccagg aatgttatca gaggagtcac ccatcagcgc caggaagtcg atgatcaatt
    14581 ctggcggcac gccgtactta ttcaccacct cttccggtcc gaggatggta ttcgtcatgg
    14641 tattgataag cgtaatattt ggcgtcacca gctgcgccat atctttatcg ccagtgctaa
    14701 tcagcaccgg acgcccggct ttttcggctt cgcgcgccag agtaccgata acgtcgtccg
    14761 cttctacgcc agaaaccgcc agcagcggca gtcccatcgc tttaaccatc gcgtgcagag
    14821 gttcgatttg tgctcgcaga tcgtccggca ttggcgggcg atgtgatttg taatgttcaa
    14881 acagttcatc acgaaaggtt tttcccttgg cgtcaaagac caccgctgca tgcgtcggtt
    14941 tatattgcat gatcagactg cgcagcatgt tgaggacacc atacatcgca ccggtcggct
    15001 cgcctgcgct gttagtcagc gggggaaacg cgtgatatgc gcgataaaga taagatgaac
    15061 catctacaag gataagtgga ttttggggga tctgaaccat aatgtccgtg cctgtttatc
    15121 agattatggg taaaggatgc cacagacagg atgaaaacat gagtttttca ccgcttttcg
    15181 tgaaaatctt ttgtagatct gctggatcgc tcgcaaatcg tcatgtggat aactttgtgc
    15241 ataaattatt tacatcgctt ttaatttatt atcatgcttt ttacagcatg atgtttgtct
    15301 ttatttttca tgcagttaaa aataaatcca tccctaataa ttgagataat gggatttttt
    15361 ctaatgtgga tagatatgaa ttatttttct ccttaaggat catccgttat ttgggtcatt
    15421 ttctgctaaa atctgcgcct tgcgcttctt tagtgcactg tctatggcta accttttgaa
    15481 taaattcatt atgacgagaa tactcgctgc gataaccctt ttgctgagta tcgtattgac
    15541 cattttggtc actatttttt gttccgtccc gatcatcatt gccgggattg taaaactatt
    15601 gctacctgtg ccagtcattt ggcgaaaggt ttcgcgtttt tgtgatttta tgatgtattg
    15661 ctggtgtgaa ggtctggcgg tattactgca tctgaaccca cacctgcaat gggaagttca
    15721 cgggctggaa gggttaagta agaagaactg gtatctgctt atttgtaacc accgtagttg
    15781 ggcagatatt gtcgtactgt gcgtgctgtt tcgtaagcac attccgatga ataaatattt
    15841 cctcaagcaa cagctggcct gggtgccatt tcttggcctg gcgtgctggg cgctggatat
    15901 gccgtttatg aagcgttatt cccgcgctta tttattacgt catccggagc gacgtggtca
    15961 agatgttgaa accactcggc gttcttgtga aaagtttcgc ctgcatccca ccaccattgt
    16021 caatttcgtt gaaggctccc gtttcacgca agaaaaacat cagcaaaccc actccacttt
    16081 tcaaaacttg ttgccaccaa aggcggcagg cattgcgatg gcgctaaatg tactgggtaa
    16141 acaattcgat aaactgctta atgttacgct gtgttatccg gacaataacc gtcagccgtt
    16201 cttcgatatg ttaagcggta aactgacgcg gattgtcgta catgtggatt tacagcctat
    16261 tgccgatgag ttacatggcg actacatcaa tgacaaaagc tttaagcgcc attttcagca
    16321 atggctgaac tcattgtggc aggaaaaaga ccggcttctg acgtctctca tatcatcaca
    16381 gcgtcagaat aagtaagttt tactcgcctc tgaatgagca gaggcgagtg agtattttaa
    16441 tgaacaaaac gtccggcacg agacataaat tcttcttcgc tcatctcctg tccgttaaaa
    16501 acaactttac ccggtgtata acgcagctgc agcgaggcgg tattgttgtc aatcgtgatt
    16561 aactgaaaca tctgacctaa tgtcatcatc ccgctgattt gtttatcagc ctgcttttgc
    16621 gctttttccg catccattcc ttcagataaa ttaagctgtt ttgcggttta tgtgacgaca
    16681 ttaagcggta acttcacatc aaaattgagc gatttgatat ctttgtttgt ggatgatgaa
    16741 gatttggctg ggtcggcaat actgatatcc agattggcat ttagttcgcc gagtgcgttc
    16801 ttccatctta ccggttgttt aatggtcggc tcactttttt gtaataacgg taaatactct
    16861 ttgaacaatg cagcattcac ttcttgcaga gcaacttcat cgtttgctaa ctcagggtgt
    16921 gcagcaagct gcttctgcat cgcaatgtta tactggatga taaattggcg cactgctgag
    16981 ggatcaatag attcagcaat caatgaccac tcaccactac cgagttgctg attccctttt
    17041 gttaacttat caagttcgta ggtcagattg acattgatat aatctttgtc tttaacgcga
    17101 tccagcctgg tttttgcaac gaaagcatcg atttgggcaa cgtcttcccc gtgattgata
    17161 atgttaatct gattaaattt cgactcgctt tcaccaaccg gaaatttctt ttcttctaat
    17221 cgcgccagag aatcaatggt aaaacttttt gccgttactt tgttcattgg tgataattgc
    17281 agaacaatat cttcgacttt gccttcaata tccaggttag acaatgaact accatcacca
    17341 ttaaaagtaa actggccctc accaaaagaa aactttccat actctgcagg ggcaacatct
    17401 aatgtcgtag agaatgagcc accaaacgcg aatcgcaatg tcgcttccac gggcgattta
    17461 tttttcgcgg cgataaatag cggttgtgtc agttcattat tcactacgtt cacttttgcc
    17521 gctgccaatg ctgggatgac attaccatgc attaacattg tgatgggcaa cggaccgtgc
    17581 tcaacatcca catcaaatac aactttctgg cctggcttga tattgagatc gggtgcaccg
    17641 ttatcgaatg tcatttgcat ttgaatacgg gaattaaaaa agcctttgtc aaagttttta
    17701 taacttaacg tcatatcgta ggcatgttca cctttctttt tcgcatcgtt aaaatcttta
    17761 ataaactttt cgataccagg ctgaatttgc gtacctgtgt accatgtgcc accaccccag
    17821 attacggcta acgcaacaat aacacctgta gctgacttac gtatcatata aatattccct
    17881 gataaaacag caaaactttg aatatccact tatgctgaaa taatgtccag cggcaggatg
    17941 cattatcaga atgtatttat atttattaaa taataaaaaa agcccgtgaa tattcacggg
    18001 ctttatgtaa tttacattga attatttttt ctcggacaga tatttcaccg tatcagcata
    18061 ctgctgaaca aaaacatcca tattgctggt atccataccc tgcggattca gctgatattt
    18121 accgttaaca aacatcgccg gaacaccacg caattgcaca tcagctgcgg ctttttcctg
    18181 ctgagcgacc agagatttca ccacgaagct gttccacgcc gcgtcgtact cttcaccttt
    18241 aatacctgca ttgataaata catcgcggat atcagatgct gaacgaatgg tctgggtttt
    18301 ctgtacgcct tcaaacagcg gaacagtcac tttgtcttcc acgcccagcg ccatcgccac
    18361 agcccatgcc tgagtcagat ctttgcccag gtcaccaccc atgaagttga cgtggtattt
    18421 agtcatcttc acgccttccg gcagtttttt cttcacatta tcagaaatat gcagaacttc
    18481 ttcaaactga tagcagtgcg ggcagaagaa agagaaaaac tccagcactt gcggcgcgcc
    18541 agctaccggt ttttccaggg tagtgtactg tttaccatct tcatactgcg ccgccgatgc
    18601 gctaaacgct aaaactaaac cagccagcgc cagccaaatc tttttcatga tctactctct
    18661 ccgattaata cataggtgtt aattgcaaag ggggttcttg tagaactttt gcctgttcta
    18721 taaaagtcgc cgtctgtcgt agccagtaat cttccccggt taaccacggg aaatttttcg
    18781 ggaacgcggg atcagcccaa tgccgcatta gccaggcaag ataataaacc aaacgcatgg
    18841 cgcgtaaagg ttcaatcagt ccgatttcag cggtgtcgaa ctcgctaaat tcttcataag
    18901 cttcaataat agtttccagt tgcatccgct gctgggcttt atcgccattg agcaacatcc
    18961 acaaatcctg aatagctgga ccattacgtg catcatccag atcaacaaac attggaccat
    19021 cgcgccagag aatattcccg gcgtggcagt ctccatgtag ccgcagaacg gtgaaatctt
    19081 cccgccagtg tgctgtaacg gcggcgatca gctcatctgt cgctttcaga aatgccgctt
    19141 tcaacccgga aggtatcagt gtagcgtcct caaacagctt gcgaggctca atgagatatt
    19201 cattcagacc gatggtctgg cgatgaataa aaagctgttt gcgccccgtc tggtgcatac
    19261 gccctaaata acgcccaacc gcttccatct gatcgatatt atcagcttcg aactggcgac
    19321 caccgacgct tggaaaaaca gcgaaataaa atccctgatg attcaataaa gtctgaccgt
    19381 taaaggccac aggtgctgcg accggaactt catcatttac cagctgcaac gcaaattgat
    19441 gttcttcgag aatttgatcg gctgtccaac gttcagggcg ataaaatttg acgacaaaac
    19501 gtcgacgctc ttcgtcctga aattgataga cacggttttc atagctgtta agcggggtaa
    19561 gaccggaatc cacccggatc ccttgctcaa acagagcgtc catgatggta tccgggtgta
    19621 gtgtctggaa agtaaaagcg ctgttgttca tcccatcatc cggaaaacat tacgaatgat
    19681 gaaggatatc atttcacgat gattttggtg gcgccgctta caagctttta ctctttaatt
    19741 acgccgcgag cgcgtaacag cgccgttttg aagtcttctt cataatcttt ctgcaaaccc
    19801 gggatcaccg catctttcgc ggaatcacgc attttcaggt gatagatcag aatgtcatcc
    19861 gtcaaatccg ccagttcgcc gtcaaaacct gactctttcg ccagtttctg caaaaattgc
    19921 aacaagttaa agtctggctc tttttgccag gctggctgga ggagttcaat aacttcattc
    19981 agacgtttac atttcatagt aatgctcctt actcttgaga cagacacgtt agcagggtca
    20041 atcccacaat aaaagaggcg atatcggtga atctgatgac gacgataaca ggcgttgtgc
    20101 tggcaggcgg taaagccaga cgaatgggcg gcgtagataa aggattgctg gacttaaatg
    20161 gcaaaccgct gtggcaacat gtcgctgacg cgcttaagac gcagctctct cacgtcgtgg
    20221 ttaatgctaa tcgtcatcag gaaatctatc aggcaggcgg tctgaaagtg attgaagatt
    20281 cactggcgga ttacccaggc cctctggcag gaatgctttc agtaatgcag caggaagctg
    20341 gtgagtggtt tttgttttgt ccgtgcgata cgccttacat tccccatgat ttagccgccc
    20401 ggcttaatca tcagcgcaaa gatgcgcctg tcgtgtgggt ccatgacggt gaacgcgatc
    20461 acccgactat tgctctggta aaccgcgcta ttgagccttt attactggaa tatctgcaag
    20521 caggagaacg ccgggtaatg gcatttatgc gtctggctgg cggtcatgcg gttgatttca
    20581 gcgatcataa agatgcattt gttaacgtga atacgccaga ggagcttgcc cgatggcagg
    20641 aaaaacgatg ataccgttac tcgcctttgc cgcgtggagt ggtaccggaa aaactacgct
    20701 tttgaaaaaa ctgatcccgg cattatgcgc cagagggatc cgtccggggc tgattaagca
    20761 tacgcaccat gatatggatg ttgataagcc aggcaaagat agctatgagc tgcgcaaggc
    20821 tggcgcggca caaaccatcg ttgccagcca gcaacgatgg gccttgatga cggaaacacc
    20881 agacgaagaa gagctggatc tacattttct cgcaagtcga atggatacct caaagctgga
    20941 tttgattctg gtcgaagggt ttaagcatga agagatcgca aagattgtgc tgtttcgcga
    21001 tgcagccgga catcgacctg aagaattagt gatagacagg catgttattg ctgtagccag
    21061 tgatgtgccg cttaatcttg atgtcgcatt actggatatt aatgatgttg aggggctggc
    21121 tgattttgtg gtggagtgga tgcaaaagca gaacggataa gcaagagtag gctggatcag
    21181 acgcagccag tgtcgcatcc gacctttttc tgcaccagcg caaaaaggcc cgactgggcc
    21241 t
//
LOCUS       DAJTXY010000065        21113 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTXY010000065 DAJTXY010000000
VERSION     DAJTXY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.065"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..848
                     /locus_tag="OO566_002465"
     CDS             <1..848
                     /locus_tag="OO566_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin"
                     /protein_id="HCR6605714.1"
                     /translation="QEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAER
                     QIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEA
                     DRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQ
                     RALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAA
                     GDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPV
                     AENVE"
     gene            1087..1146
                     /locus_tag="OO566_002466"
     CDS             1087..1146
                     /locus_tag="OO566_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6605715.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1463..1522
                     /gene="ibsE"
                     /locus_tag="OO566_002467"
     CDS             1463..1522
                     /gene="ibsE"
                     /locus_tag="OO566_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR6605716.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1642..3075)
                     /gene="hldE"
                     /locus_tag="OO566_002468"
     CDS             complement(1642..3075)
                     /gene="hldE"
                     /locus_tag="OO566_002468"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6605717.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3123..5963)
                     /gene="glnE"
                     /locus_tag="OO566_002469"
     CDS             complement(3123..5963)
                     /gene="glnE"
                     /locus_tag="OO566_002469"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6605718.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(5986..7287)
                     /gene="ygiF"
                     /locus_tag="OO566_002470"
     CDS             complement(5986..7287)
                     /gene="ygiF"
                     /locus_tag="OO566_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6605719.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIVSAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7529..8149
                     /locus_tag="OO566_002471"
     CDS             7529..8149
                     /locus_tag="OO566_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6605720.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8213..9451
                     /gene="cca"
                     /locus_tag="OO566_002472"
     CDS             8213..9451
                     /gene="cca"
                     /locus_tag="OO566_002472"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6605721.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9614..10435)
                     /gene="bacA"
                     /locus_tag="OO566_002473"
     CDS             complement(9614..10435)
                     /gene="bacA"
                     /locus_tag="OO566_002473"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6605722.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10526..10894)
                     /gene="folB"
                     /locus_tag="OO566_002474"
     CDS             complement(10526..10894)
                     /gene="folB"
                     /locus_tag="OO566_002474"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6605723.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            10999..11616
                     /gene="plsY"
                     /locus_tag="OO566_002475"
     CDS             10999..11616
                     /gene="plsY"
                     /locus_tag="OO566_002475"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6605724.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11629..12560)
                     /gene="ttdR"
                     /locus_tag="OO566_002476"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11629..12560)
                     /gene="ttdR"
                     /locus_tag="OO566_002476"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12767..13610
                     /gene="ttdA"
                     /locus_tag="OO566_002477"
                     /pseudo
     CDS             12767..13610
                     /gene="ttdA"
                     /locus_tag="OO566_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13607..14212
                     /gene="ttdB"
                     /locus_tag="OO566_002478"
     CDS             13607..14212
                     /gene="ttdB"
                     /locus_tag="OO566_002478"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6605725.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14260..15657
                     /gene="ttdT"
                     /locus_tag="OO566_002479"
     CDS             14260..15657
                     /gene="ttdT"
                     /locus_tag="OO566_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6605726.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALMLLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNN
                     TGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRYFFASATAYTSALAPMMIAAALA
                     MPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLV
                     LLVITGLLWMPVVLL"
     gene            complement(15700..16713)
                     /gene="tsaD"
                     /locus_tag="OO566_002480"
     CDS             complement(15700..16713)
                     /gene="tsaD"
                     /locus_tag="OO566_002480"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6605727.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            16951..17166
                     /gene="rpsU"
                     /locus_tag="OO566_002481"
     CDS             16951..17166
                     /gene="rpsU"
                     /locus_tag="OO566_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6605728.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17277..19022
                     /gene="dnaG"
                     /locus_tag="OO566_002482"
     CDS             17277..19022
                     /gene="dnaG"
                     /locus_tag="OO566_002482"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6605729.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19217..21058
                     /gene="rpoD"
                     /locus_tag="OO566_002483"
     CDS             19217..21058
                     /gene="rpoD"
                     /locus_tag="OO566_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6605730.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5088 a         5841 c         5420 g         4764 t
ORIGIN      
        1 aacaagaagc gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac
       61 agaatgcacg tattgcggct tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa
      121 ttctggctga acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa
      181 gaaaggttga ggctgaacgt gaagttcgta ttaaagagat cgaacagcag caggtcaccg
      241 aaatcgccaa ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg
      301 cagaagccga tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca
      361 aagcggttga actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg
      421 cggctatcgt tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac
      481 aacgtgcgct gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca
      541 aactggcctt gttgcaggcg ctacctgcgg taatagaaaa atccgttgag ccgatgaaat
      601 ctattgacgg tatcaagatt attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg
      661 atgcaaacac gggtaatgtg gggggcggaa acctagcgga acaagcatta tcagccgctc
      721 tctcttaccg cacacaggct ccgctgattg actccttgct caatgaaatt ggcgtttcag
      781 gcggttcact ggcggcattg acttcaccct taacctcaac aactcccgtc gccgaaaacg
      841 tagaataaat tttcagctat gaacgtccct ggcacaaaaa gccggggatt ttttatatct
      901 gcgttccgct aaaaactgca aatgatcagg tcgttgcaac gttttgcgtg accgctcggg
      961 gaaggcaaaa ctgcctctgg gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg
     1021 gagtcagcaa agggctgaaa cgggaaagcc cctcccgagg aaggggcctt acagaaggaa
     1081 agggttatga tgaagctcgt catcatactg attgtgttgt tactcgtaag tttcgcagct
     1141 tattaacagc caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg
     1201 ttgaaaaaaa atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg
     1261 gggatttttt atatctgcgt tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt
     1321 tgcgtgaccg ctcggggaag gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggcg
     1381 ctcatcaaca atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg
     1441 ggccttatag aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact
     1501 gttaagtttc ccgacttact aacaactgat cagagggggg agaaatcctc ccttaccctt
     1561 gttcctttac tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcga
     1621 gatctgtgaa ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt
     1681 agtggtcgag caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac
     1741 ttctttactc ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg
     1801 caggatcccg gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac
     1861 cgcttccagt gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgggaatc
     1921 ccctttcagc cgtttggtgg aggcatcgct gttcacagcg acaatcaagc ggtcacccag
     1981 tttgcgggca tttgccagat agaagacgtg tccggcgtgc aggatgtcaa agacgccatt
     2041 ggtcatcacc actttttcac cgcgtttacg cgctgccgct acggccagct tcagttcctc
     2101 ttcggtcatc acgccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat
     2161 cggcgaaacc gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa
     2221 gaagcaggct tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac
     2281 cgtgtcgccc gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg
     2341 cgctttaccc ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga
     2401 gagttcgtaa tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca
     2461 tttaccgaca acagcttcaa attccgagag attcggtgtc agcagcgtag cgccgcggta
     2521 gcgctcaaaa tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag
     2581 ttggatcatc tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc
     2641 gccaatcgaa ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacaccttc
     2701 gaaaccttct tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt
     2761 ggtgatggtc ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag
     2821 agatttactc agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt
     2881 agcaccgaga gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat
     2941 ggtattcact ttaaccacgg gcaccggcgc ttccggcgag atacgactgg tggggccgta
     3001 ccagtaacga tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg
     3061 cagcgttact ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata
     3121 cttcattctt ccaccagcca cttctgccag cttgcccgca ccagatcacg ctctgcggtg
     3181 aagcaatcct ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg
     3241 cgcagcgtcg tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt
     3301 tgcgccagca gctccagaat acgcacgttg tctgaccagc gcgttaactt cggtttttca
     3361 tgagcgtagc gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca
     3421 tcggctttga tatcaaagcg attacgatgt ttattgccga gatgagcgcg cattttctcg
     3481 cgcatttccc gcacttccgt ttgcagagtt ttaccttctc gcggcagtgt cataatctca
     3541 cgacgcaccg cgtcaaagtg cgcagtgagc tgcggatcgc cgtacactac acgcgcacgc
     3601 accagcgcct gatgttccca cgtccaggcc tcgtttttct gataatcggc aaatgctgct
     3661 gcggatgtca ccagcattcc cgccgccccg gacggacgca gtcgagcatc cacttcatac
     3721 aaaatgccgg aagaggtacg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga
     3781 taaaactgcc gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca
     3841 tggaggaaga taaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg
     3901 tagccgacca ccgcaaaacc gcgcccttca cgttcgttga ggtgatttgg cttaccgtag
     3961 cgggccacca tttgacccca cgcctgctga acgacggcat ctatcatagc ttctgccagc
     4021 caggttaagt gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca
     4081 atgcgtaaca gctgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca
     4141 tcttccggca cgcgcagcaa atactggcgc agttcatcac ggtaggcatc ggtcgccgtc
     4201 ggctggtaaa gggtatttgg atcgagcaat tcatccagca ataatggata acgcgctagc
     4261 tggctggcaa tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcaggg
     4321 aattcactga gcaattctaa ataggttgtg cgggtaacaa tccccaccag caaggcggta
     4381 atgcgcgaca gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgtggc
     4441 atcagatggt cgagcacctg acgtcctcgc ggcccgatag tacgcttatc cagctctttg
     4501 cggaaatcag caatcagcgt taggacctgt ttgcgatcat cctcgctaag atgcgccagc
     4561 actggcgtag tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac
     4621 agcgactctt cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc
     4681 acattggtca tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc
     4741 gcccacgcca gccgcgcacg gttaagctca tcagaaggaa gcgtttgggt ttgttcgtcg
     4801 ttaatgcttt gcagcaggtt ttccagacgc cgcaggaaca gatatgccac tcgcaattgt
     4861 tcagcatcgt tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa
     4921 gagcgcgact gcagcaacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata
     4981 aattcaattt cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga
     5041 cgcacttcac gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg
     5101 atgtaacgac ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct
     5161 tcgctatcgc ccataatccg cgctttgacc atcgcgtaac gctcccagtc gcgtccctgc
     5221 tcctggtaat aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac
     5281 ggacgcagtc gcatatccac gcgatagacg aagccatcct gcgttggttg atccagcact
     5341 ttaatcagcc gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg
     5401 ccctgcgtac aaccatgttc cggccaggca aaaatcagat cgatatcaga ggagaaattc
     5461 agttccccgc cgcccagctt acccatgcct aagatcagca gcggttgcgc ttcgccctgc
     5521 gcattgcacg gcgttcccca ctcgcggcag caggcgtcat acagccagtc acgcgccgca
     5581 acaatcagcg tctccgccag ataactgagc tgctgcaata tgctctcttc agtaaccagt
     5641 gccagcgttt gcgcccatgc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg
     5701 cgcattaacc cggcttcgtc gctcacatta ctgagcgcct cctgcaacca cacgctgtaa
     5761 tgttgccatt cgtcagcctg tggcggttgg ctttccagtt ccgtcagcca ctctggatgc
     5821 gcaatcacgc tgtcctgcac aaaatcactg aaagtaagta ctgacttcgc ctgtgcacta
     5881 agtgattcct cggctaaaag ctctggcagc cgctcaacaa cggtctgcca gtactgctgt
     5941 aacggtgaag agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct
     6001 gtgcaaccag aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg
     6061 ttgcccggtc gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac
     6121 gacaggatca tagtaacccg ccagcaacag tattgagtca atatcacgca tcaggcgtgg
     6181 caactggtca cggtagcgat cgcctaacgg ctggcagaaa acgcttttca gttcagcggc
     6241 atggcgggaa agatggatat cggcaaagcg tttgaaggag tcgctaattt tgccctgcgc
     6301 tttggcatct aaaaacggct gccatgcttt gcttaccagc cattcggtca acgccagctt
     6361 cgccattgcc gtttcggtag agtagaccgc cgtcacggca gaaaccgcag aaacgatggt
     6421 cgcttcgcat tgagtcagca aatcacgtaa gtgagtgctc gctttacgcg gcacaatgcc
     6481 accgaacagc atcagtgtat gacggactag gctaatggct gccagcacct gctctttcgc
     6541 cgcatcgttg ccgcgtaccc acagttcttc atgatactgc cattgcgcta acaccagctc
     6601 gagcgccgct tccagcccct gttccacatc ggcttttgcc gcaacatgca aaatggtggt
     6661 cggtttgatt tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct
     6721 caggctgccc tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc
     6781 gcgcgtgtcg ccgctaagca gctccagttc cagctcgcag ataggctcag caaattcacc
     6841 cgctttcact tccccctggt cgagagcgat ttcaattcgg ctgccatcga tctctactag
     6901 ccatttttcg cgataaaaat cggtgctaaa cagcggctgc acgcgggaag cgagatcggc
     6961 aggcaattcg ccgttcggcc agatttccgt cggtaactgc gccaggtcga gcgtcggttc
     7021 gctcaacgcc acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac
     7081 tttcatggtc atctcatagc gaccgttttc gccacgaata cgtaagccca tatcgtgcct
     7141 acgcagccag ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg
     7201 ctcgccgccc agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat
     7261 aaactttaat tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga
     7321 cgatgaacgg cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt
     7381 gccatgcaac gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca
     7441 gaggattccg attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc
     7501 cactttccat gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt
     7561 actcgcactt agcgcgactg ccgtctcaca cgctgaagaa acgcgctatg tttctgacga
     7621 actgaatacc tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa
     7681 cgccggcgag gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa
     7741 agacagctct ggccgtaccg cctggatccc gttgaaacaa cttagcactg agccaagcct
     7801 gcgctcccgt gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa
     7861 tatcgataac acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga
     7921 cagcgtgatc aacgggttaa aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc
     7981 gcagaaaaag gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat
     8041 gcaatggttt atgtatggcg gtggcgtgct ggggcttggc ttgctgctcg gtctggtact
     8101 gccgcacctg atcccaagcc gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt
     8161 cgttgcactg tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat
     8221 ttatctggtc ggtggtgctg ttcgggatgc attgttaggg ctatcggtca aagacagaga
     8281 ttgggtggtg gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg
     8341 ccgcgatttt cctgtgtttc tgcatccgca aacgcatgaa gagtatgcgc tggcacgtac
     8401 cgaacggaaa tccggttccg gttacaccgg ttttacttgc tatgccgcac cggatgtcac
     8461 gctggaagat gatcttaagc gtcgcgatct gaccattaat gcgctggccc aggacgataa
     8521 cggcgagatt atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca
     8581 tgtttccccc gcttttggcg aagatccgtt acgcgtactg cgcgtggcgc gttttgctgc
     8641 gcgttatgcc cacctcggtt ttcgtattgc cgatgaaact ctgacgctga tgcgcgagat
     8701 gacccatgcg ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaag
     8761 cgccctcacc acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggcgcact
     8821 tcgcgtttta ttcccggaaa ttgacgcact gtttggcgtt ccggcacctg ccaagtggca
     8881 tccggaaatc gacacgggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag
     8941 tccgcaggtt gatgtccgtt tcgcaacttt atgccacgat ctcggtaaag gactgacgcc
     9001 gccagaactt tggccgcgtc atcatgggca tggcccggcg ggtgttaagt tagtggaaca
     9061 actatgccag cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga
     9121 gtttcacgat ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaattatt
     9181 tgattccatc gatgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga
     9241 ggctgacgtg cgcggcagaa ccggttttga atcagcggac tacccgcaag gccgttggtt
     9301 gcgcgaaggc tgggaagtgg cgcagtcagt gccgacaaaa gcggtcgttg aagcgggatt
     9361 taaaggcgtg gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagctg
     9421 gaaggaacaa cgttgcccaa agcctgactg agtataaaac taaacgttag cacgaaccgt
     9481 cccctcgccc cttcggggag agggttaggg tgaggggaac agaccagcac cggtgcgaac
     9541 aatcaccccc accccggccc tctccctaca gagcgaggga gaaaacccgg cctgctcccg
     9601 aaccttttct caatcaaaag aacacgacat acaccgcagc cgccacaata aagcgataaa
     9661 tggcgaacgg gataaacgaa atgcgcttaa tcaattgcag gaaggtttta atcgctatca
     9721 gcgccaccac aaaagcggtg ataaacccaa cggcaaacat cgggatatcg ccggttgtca
     9781 ggaagcccca gcttttgtag agatcgagcg ccgttgcgcc catcatcatc ggtaccgcca
     9841 gcaggaacga gaactcggaa gcagcgtaac ggctcacgcc catcagcatc ccacctgaaa
     9901 tggtcgcccc ggaacgggag aaacccggcc acagcgccag acactggaaa cagccaatca
     9961 taaacgcctg acgataggtc atatcatcaa gacccggcgc acgcggctct ttcggcttca
    10021 ggcattcggc ggcaatcagc aacaaaccgc caacgaccag cgcatacatc acatttatcg
    10081 ggttaaacaa tgacttaatc gtgtcgtgga ataatagccc cagtaccacc gccggaatca
    10141 tccccagcaa aatgtggatc agcgttaaac gacctttgct ttcaccttcg tgctgcaacg
    10201 ggcggccaaa gtggatgcca atcaggccaa acagacgccg ccagaacatc actactaccg
    10261 ccagaattga tcctaactgg atcacaactt caaaggtttt cgccgtgtcg ccctcaaacc
    10321 ccaacaagtg accgacaata atcatatggc ccgtgctgga taccggcaga aattctgtca
    10381 atccttcgac cacacccaat attgccgcta tcagcagcga gtgcatatcg ctcatcaata
    10441 aacccctaaa ttattaaaat gtaccgcttg tccgaactac tgcagtatga ccaggttata
    10501 accgtttggt ttaacagctg taaaattaat tattttcttt cagattattg ccacgctcaa
    10561 tgattacgcc aacattcgcc gcccgcgcca ctgcgcctgg cttgctgagt ttgatacgca
    10621 cccacggcga gttgaagcgt gctaacagca gctccgccac ctcttcagcc acgcgttcca
    10681 ccagcgcaaa acgcgccccc tcgacgtggc tgaccaccgt ttctgcaatg tcagcgtaac
    10741 tgaggcaatc cgccacatca tcacttttcg ccgctttacg gttatcccat gccatttcga
    10801 tatcgaacac taacttctgt tcgatggtct gttcccagtc gtaaacacca atagtggtga
    10861 ttaccgaaag ttgctctata aatacaatat ccatcacgtc ctgcctgctt tttggctaac
    10921 cggataccac ttccggcgaa atgtgcgtat tatccacaga ttcatcgttg aacacgaatt
    10981 ttcaaaacgg aacagcttat gagtgcaatc gcgcctggaa tgatcctcat cgcgtacctc
    11041 tgcggctcca tttccagtgc cattctggtt tgccgcttgt gtgggctgcc cgatccgcga
    11101 accagcggct ccggcaatcc aggcgcaacc aatgtgttac gtatcggtgg caagggagca
    11161 gccgtagcag tactgatttt cgacgttctg aaaggaatgt tgcctgtctg gggcgcgtat
    11221 gaattaggtg tcagcccctt ctggctaggc ttaattgcca tcgccgcctg tcttggacac
    11281 atctggcccg ttttcttcgg ttttaaagga ggaaaaggcg ttgctaccgc ttttggagca
    11341 atcgcaccaa ttggctggga tctcaccgga gtaatggcgg gaacctggtt actgaccgtg
    11401 ctattgagcg gatactcgtc gctgggagcg attgtcagtg cactgattgc tccgttttat
    11461 gtctggtggt ttaagccaca attcaccttc ccggtttcga tgctctcttg cctgatcctg
    11521 ctgcgtcatc atgacaacat ccagcgtctg tggcgtcgtc aggagacaaa aatctggacg
    11581 aaattcaaaa gaaagcgcga aaaggatccc gagtgatttc tggtggatct acatgacctg
    11641 atagccttca tcgggcttgc ccagccgttg ctggcaccat gccgccagaa attccacgca
    11701 gacacgaatt tcatgctgcg atagagcggc tcccggtaaa cagcccagat attggcgctt
    11761 tgtgcatact ccggcaatac ttgcaccagt ttgccactct ccagaaacgg caacacatcc
    11821 cactcggaac gcagcataat ccctttgccc tccagcgccc attgcaacac tatctcgccg
    11881 ctattggagg aaagatgccc gctaaccttc accgattttt tctcctgacc gttccccaac
    11941 tcccatattc cgtgggtcat atcgcgctct ttggtcacca gacagtcatg acgacttaat
    12001 tcctgtaatg attgtggctg cggatatttt tgcagatact caggtgctgc gcataatatt
    12061 cttttatttt tcgttaacag atgcgcaata taataatcag gaatttcgtc attaatacga
    12121 atatccagat caatattatc ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatgc
    12181 acctgtaact caggataatt gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc
    12241 ccaaaaccaa aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg
    12301 acgtcatcga ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt
    12361 tggccacttt cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc
    12421 gtattttcaa ggatttggat ccgcttggtg acaaatgcag gcgtttgccc aagcgtcgcc
    12481 gctgcagcac taaaactccc gctgtggacg atttctacca gtacctgcaa gtctttggct
    12541 aaaggccagc tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg
    12601 caaaacattg ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac
    12661 gaactgttaa ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt
    12721 ttgtggcggt gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag
    12781 taataagcaa caggcagtga ataagttgac agagattgtc gctaacttta ccgccatgat
    12841 ttctacccga atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac
    12901 gtcgtcgatg gggaaaatta tctaccatac gatgttcgac aacatgcaaa aagcgattga
    12961 cctgaatcgt cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc
    13021 ccgcttccca ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac
    13081 cgtcaaagcg ccactacgtc acaatgcggt agaaattttt gacgaagtaa acaccggcaa
    13141 aaataccggt agcggcgtac cgtgggtcac ctgggacatc atccccgaca atgacgatgc
    13201 ggaaatcgaa gtttacatgg caggcggcgg ctgcacgcta cctggccgct cgaaagtgtt
    13261 aatgccgtca gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct
    13321 cgccgtaaac gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac
    13381 cgccgccgta ctctcgcgta aagccattct gcgcccgatt ggcagccgcc atcccaatcc
    13441 aaaagcggca gaactggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc
    13501 acaagggctg accggcaaca gttcagtgat gggcgcatcg tcgcggcacg ctgctggttc
    13561 atgccgatct cacctttgaa aatctgtctc acacccggag cgcgttatga aaaagatcct
    13621 gacaaccccg atcaaagctg aagatctgca agatattcgc gtcggcgatg tgatctacct
    13681 gaccggtacg ctggtgacct gccgcgacgt ttgtcaccgc cgtttgatcg aactgaaacg
    13741 tccgatccct tacgatctca acggcaaagc gattttccac gctggcccca tcgtgcgcaa
    13801 aaacggcgac aaatgggaga tggtctccgt cggcccgaca accagtatgc gtatggaaag
    13861 ttttgaacgt gaatttattg agcagaccgg cgtaaaactg gtggttggca aaggtggtat
    13921 ggggccgctg accgaagaag gctgccagaa attcaaggcg ttacatgtga ttttcccggc
    13981 aggctgcgcg gtgctggcgg caacccaggt agaagagatt gaagaagtgc actggacaga
    14041 actcgggatg ccggagtcac tgtgggtctg ccgggtcaaa gagttcggcc cgctgattgt
    14101 ctctattgat acccacggca acaacctgat agccgaaaac aaaaagctgt tcgccgaacg
    14161 ccgcgatccc atcgtggaag agatctgcga gcatgtccat tacatcaaat aaccctcccg
    14221 gagaggctca cccctctcct ttttcgcagg cataacacga tgaaaccttc cactgaatgg
    14281 tggcgatatc ttgcaccgct ggcggtcatc gccattattg ctctacttcc ggttcccgca
    14341 gggctggaga accatacctg gctctacttt gccgttttta ctggcgtgat cgttggactg
    14401 atcctcgaac ccgtgccggg tgccgtggtg gcgatggtgg gtatctccat catcgccata
    14461 ctctctccct ggctgctgtt cagccccgaa cagctcgccc agccaggctt taagttcacc
    14521 gccaaatccc tctcctgggc agtttctggt ttttctaatt cggttatctg gctcattttc
    14581 gccgccttta tgtttggcac aggctatgaa aaaaccgggc ttggacgccg cattgcgctg
    14641 attctggtga aaaagatggg gcatcgcacg ctgtttctcg gctatgcggt gatgttctcc
    14701 gagctgatcc tggcacctgt aacaccgtcc aactcggcgc gtggtgcggg tattatctat
    14761 cccatcatcc gtaacctgcc accgctctat caatcacaac caaacgacag cagttcgcgc
    14821 agcattggct cgtacatcat gtggatgggg attgttgccg actgcgtgac cagcgccatt
    14881 ttcctgacgg cgatggcacc taacttgctg ttaattggac tgatgaaaag cgcatctcac
    14941 gccacgctga gttggggcga ctggttcctc gggatgttgc cgctcagcat tttactgctt
    15001 ctgctggttc cctggctggc ttacgtgcta tacccgccgg tactgaagtc tggtgatcag
    15061 gtgccgcgct gggcagagac ggaactgcag gcaatgggcc cgctctgttc gcgtgaaaaa
    15121 cggatgctgg ggctgatggt aggcgcgctg atgctgcttc tgcgcattat cagttgggac
    15181 gacattgtca gtaataaagc ggcatggaac gttttcttct ggctggcctc gcttatcacc
    15241 ctcgcgaccg gactcaacaa taccggtttt attagctggt ttggcaaact gttagcaggc
    15301 agcttaagtg gttattcgcc gacgatggtg atggtgacgt tgattgtggt gttttatcta
    15361 ctgcgctact ttttcgccag cgccacggcg tatacctccg ccctcgcacc gatgatgatc
    15421 gccgccgcgc tggcgatgcc ggaaatcccg ctgccggtgt tctgcctgat ggttggcgcg
    15481 gcaattggtc tggggagcat tcttacacca tacgccaccg gacccagtcc gatttactac
    15541 ggtagtggtt atctgccaac ggcggattac tggcgactgg gggcgatttt tgggctgata
    15601 ttcctcgtgt tgctggtgat taccggctta ctgtggatgc ccgtggtgtt gctttaagtg
    15661 aggacggtgc cggaaattgc tctccggcac ccaatagttt tacgcagccg gtaactccgc
    15721 cagcggccag cgcggacgca cgctaacgcc gagatccgcc gtcgcgcctg ctttaaaccg
    15781 caccattccg gcataggcga tcatcgcgcc gttatcagta caaaattccg gacgcgcgta
    15841 gaacacttcg ccgcggcgtt ttttcatcat ttcagccagc ttcgcccgta acgtgcggtt
    15901 agcactcacg ccgcccgcca tgaccagtcg cttaaagccc gtctgatcca acgctcgctt
    15961 acacttaatc atcaacgtat cgaccaccgc atcttcaaag gcgcgggcga tatcagcacg
    16021 cgtctggtcg tcggtgctgt tgtcacgaat cgtgttcgcc gcaaaggttt tcagaccaga
    16081 aaagctgaaa tccagccccg gacggtcggt catcggacgc gggaaaacaa agcgcccggc
    16141 agtaccctga gccgccattt tcgacagtaa cggtccgcca ggataatcca gccccagcag
    16201 cttcgcggtt ttatcaaacg cttcaccggc ggcatcatcg atagactcgc cgagcagctc
    16261 gtactgacca atgccagtca cgctgattaa ctgcgtatga ccgccggaca ccagcagcgc
    16321 gacaaacgga aattccggcg ggttatcttc cagcatcggc gctaacagat gcccttccat
    16381 atggtgtacc gggattgccg gaacgttcca ggcaaacgcc agagagcgcc ccacggttgc
    16441 gccaaccagc agtgcgccga ctaatccagg gcctgcggta taggccacag catcaatgtc
    16501 ttttgccgtt aaaccagact cctttagcgc cgcctggatc aacggtacgg ttttacgcac
    16561 atgatcgcgg gaggccagtt caggcacgac gccgccgtag tcagcgtgca atttcacctg
    16621 actatacaat tggttggcta acaaaccttt ttcatcgtcg taaatggcga tgccggtttc
    16681 atcgcaggaa gtttcaatac ccagtacacg catgacttgt tttacctcgc tttattaccg
    16741 cgcagtgtag gaccaatgcg ggttgatgta aaactttgtt cgcccctgga gaaagcctcg
    16801 tgtatactcc tcacccttat aaaagtccct ttcaaaaaag gccgcggtgc tttacaaagc
    16861 agcagcaatt gcagtaaaat tccgcaccat tttgaaataa gctggcgttg atgccagcgg
    16921 caaaccgaat taatcaaagg tgagaggcac atgccggtaa ttaaagtacg tgaaaacgag
    16981 ccgttcgacg tagctctgcg tcgcttcaag cgttcctgcg aaaaagcagg tgttctggcg
    17041 gaagttcgtc gtcgtgagtt ctatgaaaaa ccgactaccg aacgtaagcg cgctaaagct
    17101 tctgcagtga aacgtcacgc gaagaaactg gctcgcgaaa acgcacgccg cactcgtctg
    17161 tactaattcc ccgagagcgt tgctctccga tcagaccgag ttgtagttgt aaggccgtgc
    17221 ttccgaaagg aatgcgcggc ttattttcgt ttatgaattg ctaaaaatcg gggcctatgg
    17281 ctggacgaat cccacgcgta ttcattaatg atctgctggc acgcactgac atcgtcgatc
    17341 tgatcgatgc ccgtgtgaag ctgaaaaagc agggcaagaa tttccacgcg tgttgtccat
    17401 tccacaacga gaaaaccccg tcattcaccg ttaacggtga gaaacagttt taccactgct
    17461 ttggatgtgg cgcgcacggc aacgcgatcg acttcctgat gaactacgac aagctcgagt
    17521 tcgtcgaaac ggtcgaagag ctggcagcaa tgcacaatct tgaagtgcca tttgaagcag
    17581 gcagcggccc cagccagatc gagcgccatc agaggcaaac gctttatcag ttgatggacg
    17641 gtctgaatac gttttaccaa caatctttac aacaacctgt tgccacgtct gcgcgccagt
    17701 atctggaaaa acgcggatta agccacgagg ttatcgctcg ctttgcgatt ggttttgcgc
    17761 cccccggctg ggacaacgtc ctgaagcggt ttggcggcaa tccagaaaat cgccagtcat
    17821 tgattgatgc ggggatgttg gtcactaacg atcagggacg cagttacgat cgtttccgcg
    17881 aacgggtgat attccccatt cgcgataaac gcggtcgggt gattggtttt ggcgggcgcg
    17941 tgctgggcaa cgataccccc aaatacctga actcgccgga aacagacatt ttccataaag
    18001 gccgccagct ttacggtctt tatgaagcgc agcaggataa cgctgaaccc aatcgtctgc
    18061 ttgtggtcga aggctatatg gacgtggtgg cgctggcgca atacggcatt aattacgccg
    18121 ttgcgtcgtt aggtacgtca accaccgccg atcacataca actgttgttc cgcgcgacca
    18181 acaatgtcat ttgctgttat gacggcgacc gtgcaggccg cgatgccgca tggcgagcgc
    18241 tggaaacggc gctgccttac atgacagacg gccgtcagct acgctttatg tttttgccgg
    18301 atggcgaaga ccctgacacg ctggtacgaa aagaaggtaa agaagcgttt gaagcgcgga
    18361 tggagcaggc gatgccactc tccgcatttc tgtttaacag tctgatgccg caagttgatc
    18421 tgagtacccc tgacgggcgc gcacgtttga gtacgctggc actgccattg atatcgcaag
    18481 tgccgggcga aacgctgcga atatatcttc gtcaggaatt aggcaacaaa ttaggcatac
    18541 ttgatgacag ccagcttgaa cgattaatgc caaaagcggc agagagcggc gtttctcgcc
    18601 ctgttccgca gctaaaacgc acgaccatgc gtatacttat agggttgctg gtgcaaaatc
    18661 cagaattagc gacgttggtc ccgccgcttg agaatctgga tgaaaataag ctccctggac
    18721 ttggcttatt cagagaactg gtcaacactt gtctctccca gccaggtctg acctccgggc
    18781 aacttttaga gcactatcgt ggtacaaata atgctgccac ccttgaaaaa ctgtcgatgt
    18841 gggacgatat agcagataag aatattgctg agcaaacctt caccgactca ctcaaccata
    18901 tgtttgattc gctgcttgaa ctgcgccagg aagagttaat cgctcgtgag cgcacgcatg
    18961 gtttaagcaa cgaagaacgc ctggagctct ggacattaaa ccaggagctg gcgaaaaagt
    19021 gatttaacgg cttaagtgcc gaagagcgat cgggaagccc ccgacagccg cactgagagg
    19081 cagcggcaaa tatataagta cgccctcgta attatcgttg gcggtaaaca accgttggat
    19141 ttcagcgtta acggctgaag gacatcgggt caatcgccca acaccaacct catgaaataa
    19201 gtgtggatac cgtcttatgg agcaaaaccc gcagtcacag ctgaaacttc ttgtcacccg
    19261 tggtaaggag caaggctatc tgacctatgc cgaggtcaat gaccatctgc cggaagatat
    19321 cgtcgattca gatcagatcg aagacatcat ccaaatgatc aacgacatgg gcattcaggt
    19381 gatggaagaa gcaccggatg ccgatgatct gatgctggct gaaaacaccg cggacgaaga
    19441 tgctgccgaa gccgccgcgc aggtgctttc cagcgtggaa tctgaaatcg ggcgcacgac
    19501 tgacccggta cgcatgtaca tgcgtgaaat gggcaccgtt gaactgttga cccgcgaagg
    19561 cgaaattgac atcgctaagc gtattgaaga cgggatcaac caagttcaat gctccgttgc
    19621 tgaatatccg gaagcgatca cctatctgct ggaacagtac gatcgtgttg aagcagaaga
    19681 agcgcgtctg tccgatctga tcaccggctt tgttgacccg aacgcagaag aagatctggc
    19741 acctaccgcc actcacgtcg gttctgagct ttcccaggaa gatctggacg atgacgaaga
    19801 tgaagacgaa gaagatggcg atgacgacag cgccgatgat gacaacagca tcgacccgga
    19861 actggctcgc gaaaaatttg cggaactacg cgctcagtac gttgtaacgc gtgacaccat
    19921 caaagcgaaa ggtcgcagtc acgctgccgc tcaggaagag atcctgaaac tgtctgaagt
    19981 attcaaacag ttccgcctgg tgccgaagca gtttgactac ctggtcaaca gcatgcgcgt
    20041 catgatggac cgcgttcgta cgcaagaacg tctgatcatg aagctctgcg ttgagcagtg
    20101 caaaatgccg aagaaaaact tcattaccct gtttaccggc aacgaaacca gcgatacctg
    20161 gttcaacgcg gcaattgcga tgaacaagcc gtggtcggaa aaactgcacg atgtctctga
    20221 agaagtgcat cgcgccctgc aaaaactgca gcagattgaa gaagaaaccg gcctgaccat
    20281 cgagcaggtt aaagatatca accgtcgtat gtccatcggt gaagcgaaag cccgccgtgc
    20341 gaagaaagag atggttgaag cgaacttacg tctggttatt tctatcgcca agaaatacac
    20401 caaccgtggc ttgcagttcc ttgacctgat tcaggaaggt aacatcggcc tgatgaaagc
    20461 ggttgataaa ttcgaatacc gccgtggtta caagttctcc acctacgcaa cctggtggat
    20521 ccgtcaggcg atcacccgtt ctatcgcgga tcaggcgcgc accatccgta ttccggtgca
    20581 tatgattgag actatcaaca aactcaaccg tatttctcgc cagatgctgc aagagatggg
    20641 ccgtgaaccg acgccggaag aactggctga acgtatgctg atgccggaag acaagatccg
    20701 caaagtgctg aagatcgcca aagagccaat ctccatggaa acgccgatcg gtgatgatga
    20761 agattcgcat ctgggggatt tcatcgagga taccaccctc gagctgccgc tggattctgc
    20821 gaccaccgaa agcctgcgtg cggcaacgca cgacgtgctg gctggcctga ccgcgcgtga
    20881 agcaaaagtt ctgcgtatgc gtttcggtat cgatatgaac accgaccaca cgctggaaga
    20941 agtgggtaaa cagttcgacg ttacccgcga acgtatccgt cagatcgaag cgaaggcgct
    21001 gcgcaaactg cgtcacccga gccgttctga agtgttgcgt agcttcctgg acgattaatc
    21061 ggtaggccgg atcaggcgtt acgccgcacc cggcactagt ctctctgcac aaa
//
LOCUS       DAJTXY010000066        21028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTXY010000066 DAJTXY010000000
VERSION     DAJTXY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.066"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(1..2636)
                     /locus_tag="OO566_002484"
                     /pseudo
     CDS             complement(1..2636)
                     /locus_tag="OO566_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380005.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            complement(3096..3269)
                     /locus_tag="OO566_002485"
     CDS             complement(3096..3269)
                     /locus_tag="OO566_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605731.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            3387..4580
                     /locus_tag="OO566_002486"
     CDS             3387..4580
                     /locus_tag="OO566_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6605732.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4778..6031)
                     /gene="glyA"
                     /locus_tag="OO566_002487"
     CDS             complement(4778..6031)
                     /gene="glyA"
                     /locus_tag="OO566_002487"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6605733.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6358..7548
                     /gene="hmpA"
                     /locus_tag="OO566_002488"
     CDS             6358..7548
                     /gene="hmpA"
                     /locus_tag="OO566_002488"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6605734.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7593..7931)
                     /gene="glnB"
                     /locus_tag="OO566_002489"
     CDS             complement(7593..7931)
                     /gene="glnB"
                     /locus_tag="OO566_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6605735.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7992..9326)
                     /gene="glrR"
                     /locus_tag="OO566_002490"
     CDS             complement(7992..9326)
                     /gene="glrR"
                     /locus_tag="OO566_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6605736.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9316..10029)
                     /gene="qseG"
                     /locus_tag="OO566_002491"
     CDS             complement(9316..10029)
                     /gene="qseG"
                     /locus_tag="OO566_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6605737.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10194..11621)
                     /gene="qseE"
                     /locus_tag="OO566_002492"
     CDS             complement(10194..11621)
                     /gene="qseE"
                     /locus_tag="OO566_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6605738.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12179..16066)
                     /gene="purL"
                     /locus_tag="OO566_002493"
                     /gene_synonym="purI"
     CDS             complement(12179..16066)
                     /gene="purL"
                     /locus_tag="OO566_002493"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6605739.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16324..17880
                     /gene="mltF"
                     /locus_tag="OO566_002494"
     CDS             16324..17880
                     /gene="mltF"
                     /locus_tag="OO566_002494"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6605740.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17877..18413)
                     /gene="tadA"
                     /locus_tag="OO566_002495"
     CDS             complement(17877..18413)
                     /gene="tadA"
                     /locus_tag="OO566_002495"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6605741.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18438..19073)
                     /gene="yfhb"
                     /locus_tag="OO566_002496"
     CDS             complement(18438..19073)
                     /gene="yfhb"
                     /locus_tag="OO566_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6605742.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19282..20130
                     /locus_tag="OO566_002497"
     CDS             19282..20130
                     /locus_tag="OO566_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6605743.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20469..21023)
                     /gene="pinE"
                     /locus_tag="OO566_002498"
                     /pseudo
     CDS             complement(20469..21023)
                     /gene="pinE"
                     /locus_tag="OO566_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT         5053 a         5679 c         5353 g         4943 t
ORIGIN      
        1 aacgtagtta aaaataaaag gcatcacagt ggtgaataat gccgctttcg gccaggccgg
       61 attaatcgcc gtcaattcag cgcgagcaga ctcatattgc gcaacctccc cgagtcccgc
      121 ggacgccagc atcgccacca tcaggcattt ttctttatgc tgctgttgta aatcgccata
      181 cagcgataac aggtcaggtt gtgaaacagc aaagaaatcc gcctcaatac tggttttcgc
      241 catctcttgc gcccactgtt tcatttcact aaacagttgc tgtgcggttt gctgttcacc
      301 cagcagtcgc agcgccattc cttgccagaa gagataatca accggctgat cgttgtaata
      361 actgtggatg ttaatggtgc gatcgccggt cgccgccaga cgtaaacaac gcatcgcttc
      421 agtttcatcg ccctgcgcgt tggcgcatat cgcctgccag aaccagatgt cgttatcagt
      481 ttgccccggt aaacggcctt cgcttaaatt ctccggataa tgcagcgcgg catgaagcag
      541 ttcgctggcc tgctgcggct ctctggcatc aagatgctgc caggcgcgta ataactggtt
      601 gaggataaac tgactggtga ccttcccttc cccgccttcc cacgggtgga atttacgcgt
      661 ggcgagaatg tccgccgctt tgtctgcctg acccgtgaga tgccacaaat tgagcagttc
      721 tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta ttttccagac gcgccagtcg
      781 tttctccggt gtggctccac ttaacttatc cagcaaatcc cgttcgaaaa gcagacgtgc
      841 atcctgcggc gcaagctgat aagcattatc aagataacgc gcggccagct cataatcgtg
      901 tttcttattc cacgcatgga tcgctaaccc gcgccagccg tcggcaaact ccggcgacat
      961 ctctacgcaa cgttgccata aggcaatggc tttgttgtag ctacgtttgt tgtagtagaa
     1021 gcaggccagt aaatggcgag caaaccagca ctcttcaata ctctccagcg ccgccacttc
     1081 ttccagcgta ttcgggaaac ggacaaactg cgggaagaca tcaatggctt ttgcgaccag
     1141 ttcgccacgt tcggctttcg gcagcaagct ggcttgcagg taaagcggca gcgtgcgctg
     1201 gcagtccagt gcgttcagca tctctgccgc cagagtgggc attccccagt taatcagttg
     1261 cccggcggtc atcagagcgt taacgtcgcg tccctgacac agaccgcgcc actgcgcgag
     1321 ggctgattcg ctacgaccat cgaaccagtt cagccaccac agagtggcgt tcagcggata
     1381 atcacgcagc agtttctcgc gctgcaaacg cgcgttgtcc tgacgaccac ttaacaccag
     1441 cagcagatta tgcaagcaaa gcacttcctg attggttggg caggcacgaa gactttgttg
     1501 gcaaaaatcc agacccgcgt cgaagttatc attacgcgcc gccagtcgtg ccagaccata
     1561 atagccgcct gctttactat tgccgctcca gaccgcacgc cagaaatcct cttcggcttg
     1621 ttgatattgt ccctgacgtt cgtaagcact ggcgcgaatc aaacttgctt gtccacactg
     1681 cgggtttttg ttcagcgcat gtgcgcgtcc tgactggcat acgccaccgc ttgcgggaaa
     1741 tctgcgcggt tatattccag catcgccagc gccaggttac agcgataatc cagcgggtcc
     1801 agcgccacgc cgcgcaggta gtaatcgaac ggtgaacggc tggcgtgatg atattgctcc
     1861 agatgctgac cgataaacca ggcttcatct gtactggtaa tgtcttgtgc tgccagtggc
     1921 gctttggcga cgtccggcaa cggcaactcc tgcggctgat gttcctgata actcagtaca
     1981 atattgccgt cggcatcgga gagttcaatg gtcagtctgt caggattgat accgtgcaac
     2041 acgccctgga tggcggtcgc tggtgtcagg gccacggcat catcgagcaa cgcgttgcat
     2101 ttgccgattt cgcggatcgc cagacggtat ccgttcaacg gagagatggc atacagcccc
     2161 cactcaatcc cccgctcact acgctggagt tttatcaccg catcgcggga ggcattttgc
     2221 accatgcaca gagaatgata aggcaggaaa tactgctcga aacgcttctc ttcgtaagca
     2281 tcaagccagg taaaatcagg ctggttatcg gcaaaaatac cggtcatcag ttcgatatac
     2341 gggccgttat tgtcggtcag actcttatcc cacgcctggc caaattcact gtgtccccaa
     2401 ctccactgtt ttttacctgg cgcaatatgg tggttggcaa cgtgcagcaa accgccatct
     2461 tcatcgtgac accacgcgcc gacaaaatcg tactgcgatt tttcggccat atatgaggtt
     2521 ggaacaggca catttttata gcgagaaatg tccactccgg cggagtagtc cactttgtag
     2581 taagtgccgg tggcgatggg gaaagcggag acggcccgtt tgccgtgatc aaacaccgca
     2641 gtcacatccg gtgggaaaac gctctgatgc ccttcacccc ctttcactgc cgggttggcc
     2701 caccacaaga aatgacgcgg cgtggcgttg ccgttataga cgcggctggc gatttccagt
     2761 gccgcccggt cagggcgcag ggtgaaacct gtcatcacct gtaaaccatg catcggctcc
     2821 gtttcgccga cccacaccgt ctgtgcaccg tcttcatggg cttcgagggt gaaatcaacg
     2881 ggcataaagg tggtcgggcg atggtgttgc ggccagttaa actcaatccc gccggagatc
     2941 cacggtccca gcagccccac cagcgcaggt ttaatgactt cattgtgata gacaaaatcg
     3001 cgctgtttca ctttatccca tgcgcgatgc acccgaccgc ccagttccgg caggatcatc
     3061 actttgatgt agtcgttttc cagccacacc gcctgctagg atttcagggt tttctgctcg
     3121 ctcagcgtat cggtcacgcc gtagggataa accgcgccgg acgatccctg ataaacgcga
     3181 ttttccagga acatgggatg tatatcctgc ggcccggttt cataggtcgg gatctcaacg
     3241 cgctcttgcc acacttttac tggagtcatg gtgccctcaa tattaacaag acatactgaa
     3301 ttaaaagatt tgtggcagtg tattgaacaa tctggcaatg ttttcgcgga ataatcacgc
     3361 aattaactaa acaaggttta gtgaagatga gagcctgcat taacaatcaa cagattcgcc
     3421 accataacaa atgcgtgatt ctggaactgc tgtaccggca aaagcgcgcc aataaatcaa
     3481 cgctggcccg gctggcgcaa atttcgattc cggcagtcag taatattttg caggaactgg
     3541 aaagcgaaaa acgggtggtg aatattgacg atgaaagcca gacgcgcggg catagtagcg
     3601 gtacatggct gattgcgccg gaaggtgact ggacgctgtg cctgaacgtg acgcccacca
     3661 gtattgagtg tcaggttgct aatgcttgtt taagtccgaa aggcgaattt gagtatttac
     3721 agattgatgt accgacgccg caggcgctgc tgtccgaaat cgaaaaatgc tggcatcgcc
     3781 accgtaaatt gtggccggac cgcaccatca atctggcgct ggcaatccac gggcaggttg
     3841 atccggtgac cggcgtatcg caaaccatgc cgcaagcgcc gtggacaacg ccagttgagg
     3901 tgaagtatct gctggaagag aagctgggca ttcgggtgct ggtcgataat gactgcgtga
     3961 tgctggcgca ggcggagaaa tggcaaaata attcgcagga acgggatttc tgcgtgatca
     4021 acgttgatta cggcattggc tcgtcgttcg tgattaacga gcaaatttat cgcggcagtt
     4081 tgtatggtag cggacagatt ggtcacacca tcgttaatcc ggatggcgtc gtctgcgact
     4141 gtggacgtta cggctgcctg gaaaccgtcg cctcgttaag tgcattaaaa aaacaggcgc
     4201 gggtatggct aaaatcacaa ccggttaata ctcagcttga tcctgaaaaa ctgacaaccg
     4261 cgcagttaat cgctgcctgg cagagtggag aaccgtggat caccagctgg gttgatcgct
     4321 ctgccaatgc cattggtttg agtctgtata acttcctcaa catcctcaat attaatcaga
     4381 tttggttgta cggtcgcagt tgtgcctttg gtgagaactg gcttaatact attattcgcc
     4441 agacaggatt taacccgttc gaccgcgacg aaggaccgag cgtgaaagcg acgcaaattg
     4501 gcttcgggca attaagccgc gcacaacagg tgctgggaat tggctatttg tatgttgagg
     4561 cgcagttacg acagatttga tggcgcgata acgtagaaag gcttcccgaa ggaagccttg
     4621 atgatcataa acgaaaaatt gcctgatgcg ctacgcttat caggcctaca cggagattgc
     4681 aatatattga atttgcaaga ttttgtaggc ctgagaaggt gttcatgccg catccaacat
     4741 gaacaacgag cacattgtca gcaaatcacc gtttcgctta tgcgtaaacc gggtaacgtg
     4801 cgcagatgtc gagaacttta cctttgatgc gctcgataac ggcttcatca ttgatgctgt
     4861 ccagcacgtc acacatccag ccagccagtt ctttcgcttc agcttcttta aagccgcgac
     4921 gggtaatcgc cggagtacct acacgaatac cggaggtcac aaacgggctc ttcggatcgt
     4981 tcggtacgct gtttttgttg acggtgatgt tagcacggcc cagagcggcg tctgcttctt
     5041 taccggtcag gtttttatca accagatcaa ccaggaacag gtggttatca gtgccgccgg
     5101 aaaccacttt gtagccgcgc tcgaggaaca cttctaccat cgctttagcg tttttcgcga
     5161 cctgctgctg gtaagttttg aactcaggct ccatcgcttc tttcagagca accgctttac
     5221 cggcgattac gtgcatcaac ggaccgccct gaccaccagg gaaaacggca gagttcagtt
     5281 ttttgtacag ctcttcgcta ccacctttcg ccaggatcag gccgccgcgc ggacccgcca
     5341 gggttttgtg agtggtggta gtaacaacgt gagcatgagg aaccgggttc gggtagacgc
     5401 cagcagcaac caggcccgca acgtgcgcca tatcaacgaa cagataagca ccgatgctgt
     5461 cagcgatttc acgcattttc gcccagtcaa ccacgccgga atatgcagag aaaccaccga
     5521 taatcatttt cggcttgtgt tctttggctt gtttttccag atcggcgtaa tcgatatgac
     5581 cggtagcatc gataccgtaa ggaacgatgt tgtacagttt accggagaag ttaaccggag
     5641 aaccgtgagt caggtgaccg ccatgcgcca ggttcatacc cagaacggta tcacctggtt
     5701 ccagcagcgc ggtgtagacc gcaaagttag cctgggagcc ggagtgcggc tggacgttag
     5761 cgtagtcagc gccgaacagt tctttcgcac gatcgatcgc cagttgttca acgatatcaa
     5821 catactcgca accgccgtag tagcgtttgc ccggataacc ttcagcatat ttgttggtca
     5881 gctgagaacc ctgcgcctgc attacgcgcg ggctggtgta gttttcggag gcgatcagtt
     5941 cgatgtgctc ttcctgacgt actttttcct gctccatagc ctgccacagt tcggcatcat
     6001 aatcggcaat gttcatttca cgctttaaca tccgcatctc ctgactcagc taacaataaa
     6061 atttttggcc tttataggcg gtcctgttgg acaacggcga acagtataac cgaatcattg
     6121 tgcgataaca ggtcttgaca aaggaattta cgcaaacgat taccttaagg ctacgcaagg
     6181 ctttggagaa taaagagctt gcaaccggaa acggatttct tttcaggttt gtgatgcaaa
     6241 tttttcactt catcacattc tttctgaaaa acaccaaaga accatttaca ttgcagggct
     6301 attttttata agatgcattt gagatacatc aattaagatg caaaaaagga agaccatatg
     6361 cttgacgctc aaaccatcgc tacagtaaaa gccaccatcc ctttactggt ggaaacgggg
     6421 ccaaagttaa ccgcccattt ctacgaccgt atgtttactc ataacccaga actcaaagaa
     6481 atttttaaca tgagtaacca gcgtaatggc gatcaacgtg aagccctgtt taacgctatt
     6541 gccgcctacg ccagtaatat tgaaaacctg cctgcgctgc tgccagcggt agaaaaaatc
     6601 gcgcagaagc acaccagctt ccagatcaaa ccggaacagt acaacatcgt cggtgaacac
     6661 ctgttggcaa cgctggacga aatgttcagc ccggggcagg aagtgctgga cgcgtggggt
     6721 aaagcctatg gtgtactggc taatgtattt atcaatcgcg aggcggaaat ctataacgaa
     6781 aacgccagca aagccggtgg ttgggaaggt actcgcgatt tccgcattgt ggctaaaaca
     6841 ccgcgcagcg cgcttatcac cagcttcgaa ctggagccgg tcgacggtgg cgcagtggca
     6901 gaataccgtc cggggcaata tctcggcgtc tggctgaagc cggaaggttt cccgcatcag
     6961 gaaattcgtc agtactcttt gactcgcaaa ccggatggca aaggctatcg tatcgcggtg
     7021 aaacgcgaag agggtgggca ggtatccaac tggttgcaca atcacgccaa tgttggcgat
     7081 gtcgtgaaac tggtcgctcc agcaggtgat ttctttatgg ctgtcgcaga tgacacacca
     7141 gtgacgttaa tctctaccgg tgttggtcaa acgccaatgc tggcaatgct cgacacgctg
     7201 gcaaaagcag gccacacagc acaagtgaac tggttccatg cggcagaaaa tggcgaagtt
     7261 cacgcctttg ctgatgaagt taaggaactg gggcagtcac tgccgcgctt taccgcgcac
     7321 acctggtatc gtcagccgag cgaagccgat cgcgctaaag gtcagtttga tagcgaaggt
     7381 ctgatggatt tgagcaaact ggaaggtgcg ttcagcgatc cgacaatgca gttctatctc
     7441 tgcggcccgg ttggcttcat gcagtttacc gcgaaacagt tagtggatct gggcgtgaag
     7501 caggaaaaca ttcattacga atgctttggc ccgcataagg tgctgtaatt tgatgttgcc
     7561 ggatggaaac atccggcaac ccttgacgcg gtttaaattg ccgcgtcgtc ctcttcaccg
     7621 gtacggatgc gaatgacccg agccacgtca aagacgaaga ttttaccgtc accgattttg
     7681 ccggtttgcg ccgtgcgaat aatggtatcg acacaggtat cgacaatgtc gtccggtacg
     7741 acaatctcaa ttttcacttt cggcagaaaa tccaccatat actccgcgcc gcggtacagc
     7801 tcggtatggc ctttctggcg accaaagcct ttcacttcgg tcaccgtcat gccggtaata
     7861 ccgacttcgg ccagtgcttc gcggacatcg tccagcttga agggttttat aatcgcatca
     7921 atctttttca tgctattcct tgaaaaggtt gtctgtcttt tgatctgcta aacgtaacac
     7981 ataacgccaa ttcattcctt gaaatcgttt gcatccagct cgtgtcggga aagcagttta
     8041 taaaattctg tccggttgcg ccccgccatt ctcgccgcgt gggtgacgtt gcctttggtg
     8101 atttgcagca gcttacgcaa atagttgagt tcaaaatgat tacgtgcctc aacaaaggtt
     8161 ggcagcgccg tattttcacc ctccagcgcc tgctccacca gcgcatcact aatcaccgga
     8221 gatgaggtca gcgccacgca ctgttcaatc acgttgacca actggcgcac attacccggc
     8281 cagctcgcgg tcatcaggcg tttcatcgca tcggtagaga acgcgcggac aaacggttta
     8341 tgtcgctctg ccgcctggcg caacaggtga tttgccagta gcggaatgtc ttctgtgcgc
     8401 tccgccagtg ccggaatttt caggctgaca acgttgaggc ggtaatagag atcttcgcgg
     8461 aattccccgc gcgccatcgc ttttgacaga tcacggtgag tggcagaaat aatccgcaca
     8521 ttgatatcaa tatcgcggtt actgcccagc gggcgcactt tacgctcctg caacacgcgc
     8581 agcagtttga cctgtaacgg cgcaggcata tcgccaattt catcgagaaa tagcgttccg
     8641 ccttccgccg cctggaataa accttcgcga ttgctgacag cgccagtaaa cgcgccacgc
     8701 gcatgaccaa acagctccga ctccagcaat tgctcgggca atgcgccaca gttaatagca
     8761 ataaatggtt tgctgttgcg cgggctggcg ttgtggatag cctgggcgaa aatctctttc
     8821 ccggtgccgc tctgaccgtt aatcaaaacg ctgacgtctg attgcgccac cagccgcgcc
     8881 tgttccagca aacgcagcat cagcgggctg cgggtgacaa ttgcctcgcg ccagcgttca
     8941 tcggtggctg gcgcggattg ctccagcgca tcgtcaattg cctgatatag cgcatcttta
     9001 tcgacaggct tggtgaggaa actaaaaacg ccctgctgtg ttgcagcaac ggcatcgggg
     9061 atagaaccat gcgcagtaag aataattact ggcattcccg gctgcacttt ctggatttca
     9121 gcaaatagct gcataccgtc catttcatcc atccgcaggt cgctgataac taaatctact
     9181 ttttcgcgat tcagtacccg taatccttca gcgccacttt ccgccgtgac cacactgtag
     9241 ccttcgctgg tcaggcgcag gccaagcagt ttcagcaatc ccggatcgtc atcgaccaat
     9301 aataaatgcg caggtttatg gctcatcagg agtgacctca tgggtggatg gcgcgggctt
     9361 ttcactctcg tgcggcgtat ccggcgagaa atttccagcc ggtttgcggg tcgagagctg
     9421 gcgttcaata tcggtcaggt tttccagctt gcgggtggtg agttccagtt gctgctgtag
     9481 aacgtgatgt tgctggcgca atgtatccag ctcgctgtcg ctggactgct ggagtttact
     9541 gtaacgttgg cgctcttccg ccagttgcag ttgcagcgcc tgaccgtcgc gccagagttg
     9601 atacagtggg cgaacctgtg ccgggatctc ggtacttaac gcttcaatac gcgcgaccag
     9661 ctggcggcgc tcatacggcg taattttggc gtcggcgagc aaaatccctt gtttaaaggt
     9721 attttgccag ctgccgtcgt catattggcg agcttgctga cgcgactgcg caggcattaa
     9781 acgatcagca caatccatcg cccgcagcca gtaaagcgga ttggtttcgg ttgatttacc
     9841 ttgcagcgcc cagatgtcgc tacattcagt agaaagatag tcagccagtt gataaaccgg
     9901 aattttttct tctgctggcg tatcaatggc tggcttattg tgattctgca cgcaacccag
     9961 caatgccaga catggcagcc ctgccagcca cagccgtcgg ggcaataatc gttgaaaaat
    10021 gtgtcgcata ttcaccagac ttaaagccta tcccagtggg cgtaattgtt gcagacagtc
    10081 tggacatgga cagcgcggag aaaccggggc gtacatatcg tacgtgaaga tttcgagcac
    10141 tacccggggc cgaactgaca aataaaatag cctgatagga taggctgtta gcattatttc
    10201 gtgtttttcg acgacggtaa ttcaatgcgg aaacaaacgt cttgcccgct ctcgtcgacc
    10261 agatacagtt ccccttgcat acggcgaata caatccctgg caatgcttaa tcccagaccg
    10321 ctgcccttca ccgccccttt tcgctggtgg cttccctgaa aaaagggttc gaagatcatg
    10381 gcgcgttcct cttgcggaat gggcgtgcct gtattgatga catcaatata aacccgcgca
    10441 ccatgtaaac tgctgcgaag gcaaatgtta ccggattcag ccccgtagtg caccgcattg
    10501 gagtaaagat tatccagtac gctcatcagc agcattggct ccgccaagca agctgttgct
    10561 ttgagatcga cgtcggtatg catcatttta gcccgtgcgg gcaggctatg agcagaaacc
    10621 actgtctcca ccagcggtgc taattcaaca ttctccagtt ccaccgcact gtccgcctgt
    10681 ttacggttgt aatcaagcag ttgttcgatc agtttttgca aattgcagct gctgctatca
    10741 agaatgctca ccacctcttt ttgctctggc gtaagcggcc cgacaacctg atcagccagt
    10801 aattcagtgc cctcgcgcat actcgccagt ggcgttttta attcatgaga taaatgtctt
    10861 aaaaattgat ggcgttggga ttccagccat gacaggcgct cacttaacca aagaatacgt
    10921 tgcccaaccg agcgtaactc gctcggtcca cagaacgaaa cgctattgcc cagagaacgc
    10981 ccttccccca gacggttgat catgcgctcg atatttttca ccggcccgat aatcatccgc
    11041 gtgaaaagta gcaccatcac cagactcacc agaaatagca ccagcgattg ccaaccaaaa
    11101 tattgcccac gttcggcgat ttcacgctga agttgctgcc cacgagagaa caccactgtg
    11161 cgcgtggcct gcaccatttc ggtattggca ctggcaaagg cttcaagacg cgcggcggcg
    11221 gcagcatcgg gaccactgtt gttacactga agttgggcca gattgttcaa atcctgacgc
    11281 aatgcctggt aaagtttatc gtctggcagt acgcctgcgt gggcatcgag catttcgctg
    11341 taacgcttgc gctggctttg ataaaccttc gcaagcgtag ggtcgtccag cacgcaatac
    11401 tgacggtagc tacgctccat ctccagcgcc gcgttggtca tcgcttcact acgccgggca
    11461 tcaataagcg tagtgcggtt aaccagcgcc gcctgatcgc taagcgcatt caggctttgc
    11521 caggcttgcc atgccagcac caacaggggt agtagaatta gcaaaaatgc cagcattacc
    11581 agttgtcgta atgagcgggg aaaaacgggc cagcgtttca aggtgttact ctcgtcagac
    11641 gcgaatagcc tgatgctaac cgaggggaag ttcagataca acaaagccgg gaattacccg
    11701 gctttgttat ggaataaggc ggtgcctaac tcgacgtttc gcccgatggt tgatatagct
    11761 acgctgatat cagaagttgg acggcaggca ccttgttgtg cgtcattcga attttatgta
    11821 gcacgtcccg aaggggctga cataagtcgg tgaatgagcc actgcttact attatgcagt
    11881 aactgtgcca ataaagaaaa tagtttggta acgtactgat tatacgttgc tttgagagat
    11941 ttatggcttc ttcgctattt gaatgtaaat cagccactgt gtcgctaaaa agagacaact
    12001 taagataaac ttattagatt acaataaaat caatcattta agtgtcgcca gaaagcgaca
    12061 cggtaaacca cccattgtcg tgatttacag acacaaaaaa gctcccagag ttgggagctt
    12121 atgatagtgg ttggtgctta atgccgcatc cgggctgcaa aaccaatgaa ctgatgactt
    12181 accccaactg cttacgcgca ttgcggaaaa tgcgcatcca tgggccatcc tcgccccagt
    12241 tttccggatg ccaggagttg ctgacagtac ggaaaacacg ttccgggtgc ggcatcataa
    12301 tggtgactcg accactttca gtcgtaaccg ccgtaatacc gttcggtgaa ccgttcgggt
    12361 tagccgggta ggtttcagtg actttgccga agttatcgac atagcgcagt gccaccagcc
    12421 ctttgctttc cagtgccgcc agatgcgccg catcacgcac ttccacgcgc ccttcaccat
    12481 gagagacagc aatcggcatt tgcgagccca ccatcccctg caacagcaga gacgggcttt
    12541 gggttacttc aaccaggctg aaacgcgctt caaagcgatc ggaggtgtta cgcacaaaac
    12601 gtggccacaa ctcgctcccc gggatcagtt cacgcaggtt agacatcatc tggcaaccgt
    12661 tacatacccc cagggccagc gtttgcggac gatggaagaa ggttgcaaac tcatcgcgta
    12721 cacggtcatt gaacaggata gacttcgccc aaccttcacc ggcacccagc acatcaccgt
    12781 aggagaaacc tccgcacgcg accagggcgt gaaaatcctc cagtcccgta cgtccagtca
    12841 gcaggtcgct catatgcacg tcgatagcat caaagccccc acgttggaaa gctgccgcca
    12901 tttcaacatg cgagttcacg ccctgctcac gcagtacggc aactttcgga cgtgcgccag
    12961 tggcaatata cggtgctgcc acatcttcgt tgatatcgaa cgacagtttt acattcaggc
    13021 ccggatcggc gtcgttagat ttcgcctgat gctcctgatc ggcacactcc gggttgtcac
    13081 gcaggcgctg catctgccag gtagtttctg cccaccagac acgcaacgta gtgcggcttt
    13141 cgctgaatac agtctgcccg ttggcggtaa tcacaaaacg gtcaccggaa accgcctgcc
    13201 caacataatg gacgcaatcc gcaagcccat gctgtgccag tacggactcg accgcttcac
    13261 ggtcagcggc acgaacctga atcaccgcac ccagttcttc gttaaacaac gccgccaggc
    13321 gatcgtcacc cagagaggcg atatccgcgt caatgccaca atgacctgca aaggccattt
    13381 ccgccagcgt taccagcagg ccgccatcag agcggtcgtg atacgccagc agcttacgct
    13441 gtgcaaccag cgcctgaatc gcgtcataga agcctttcag ttggacaaca tcgcgtacat
    13501 ctgccggttt gtcgccaagc tgacgataaa cctgcgccag cgccgttgcg cccagcgcgt
    13561 tattgccttt gcccaaatca atcagcagca gtgcgttatc ttcggtacaa agctgcggcg
    13621 tgatggtgtg acgtacatct tccacgcggg caaatgcaga aatcaccagc gacagcggcg
    13681 acgtcatttc gcgctcttcg ttaccttcct gccagcgggt tttcatcgac atagagtctt
    13741 tacccaccgg gatcgtcagg cccagcgccg gacaaagttc ttcacccacg gctttaacgg
    13801 cttcatacag gcccgcatct tcaccagggt ggcctgccgc cgccatccag ttggcggaaa
    13861 gtttaatgcg tttgatatcg ccaatctgtg ttgcggcaat attggttaac gcttcaccga
    13921 ccgccagacg ggcagaggcg gcgaaatcca gcagcgcaac cggcgcacgc tcgccaatcg
    13981 ccatcgcttc accgtagtag ctgtcgaggc tggcggtagt gaccgcgcag ttagcgaccg
    14041 gcacctgcca cggccccacc atctgatcgc gcgccaccat gccggttacg ctgcggtcac
    14101 cgatggtcac caggaaagtt ttctccgcca cggtcggcag atgcagtacg cgtttcaccg
    14161 cgtctgcaat ggtgatccct tcacggacca gcgcatcgcc cttcgctttc agcgtttgta
    14221 catcgcgggt catcttcggc gttttaccaa gcaggacgtc cagcggcaga tcgatcggct
    14281 gattatcaaa atgacgatcg tgcagagaaa gatgcagttc ttcggtcgct tcaccaatca
    14341 ccgcgtaggg tgcgcgctca cgcttacaca gttcgtcaaa cagcggtaat tgatcggcag
    14401 caaccgccag cacgtagcgt tcctgggatt cgttacacca gatttccagc gggctcatgc
    14461 ccggttcgtc gcttagaata tcgcgcagtt caaatttacc gccgcgtccg ccgtcgctca
    14521 ccagctccgg catcgcgtta gaaagaccgc ccgcaccgac gtcgtggata aacagaattg
    14581 ggttagcatc accaagctgc cagcaacggt cgatcacttc ctggcaacga cgctccatct
    14641 ccgggttgtc gcgctgtacg gaagcaaagt cgaggtcggc atcagactga ccagacgcca
    14701 tggaagacgc cgcgccgccg ccaagaccga tgttcattgc cgggccgccg agaacgacca
    14761 gcttcgcacc gacgttgatc tcgccttttt gtacgtgatc ggcgcgaatg ttgccgatcc
    14821 cgcccgccag catgatcggt ttgtgataac cgcgcagctc ttcgccattg tggctgttca
    14881 ctttttcttc ataagtacgg aagtagccgt tcagtgccgg acgaccaaat tcgttgttaa
    14941 acgccgcacc gcccagcggg ccttcggtca tgatgtccag cgcggtaaca atgcgctcag
    15001 gcttaccgaa atcttcttcc cacggctgtt cgaagccagg aattcgcagg ttggatacgg
    15061 agaaaccaac caaaccagct ttcggcttcg cgccgcgccc ggtggcacct tcatcgcgga
    15121 tttcaccgcc ggaaccggtc gccgcgcccg gccacggaga aatcgccgtc gggtggttgt
    15181 gagtttcgac tttcatcagg atgtgcgccg gttcctgatg gaaattgtag cggcccgttt
    15241 cgtggtcagc aaagtagcgg cccacttcag aaccttccat tacggcggcg ttatctttat
    15301 aagcagagag aacgtgatct ggcgtggttt cgaaagtatt tttgatcatc ctgaacagcg
    15361 atttcggctg ttgttcaccg tcgataatcc agtcggcgtt aaaaattttg tggcggcagt
    15421 gctcagagtt cgcctgagcg aacatataca gctcgatgtc gttcgggtta cgaccaagct
    15481 ttgtgaaagc atcctgcaga tagtcaattt catcttccgc cagagccagg ccaagacgca
    15541 ggttagcgtc gatcagcgcc tgacggccct gccccagcaa atcaacgctt gtgaccggag
    15601 tcggttgatg gtgggcaaac aactgctctg catcatctaa atcaaaaaag accgtttcca
    15661 tcatgcggtc gtgcagttca gcggtaacct gctgccattg ttcattggtc agcgtaccgg
    15721 cttctatata gtaagcaacg ccgcgctcaa ggcggtttac ctgttgtagc ccgcagttat
    15781 gggcaatatc ggtcgctttc gaagaccagg gagagatggt gccaggacgc ggggtcacca
    15841 gcaggagttt gccttgcggg gcgtggctgg cgagtgccgg accatatttc agcaggcgtt
    15901 caagttgtgc gtgctcatca tcgtttaacg gcgcattgag gtcagcaaaa tggacatact
    15961 cggcgtaaat attgtgaacc gggagcctgg cagcctgaaa acgtgccagc agtttgttga
    16021 ttcggaatgc cgacagtgca ggcgaaccac gcagaatttc catcataagt ctctcgtctt
    16081 ctaagctttc ggtgtaccca agggggggaa acgggcgtca ttataaagaa tctgatgcgc
    16141 tgacgaaacc gtttgcgtgg aaataaaatc accatcgtga attagcaacg cgtgccgcca
    16201 atggctgtaa taagttgcca tctggcgcag gtttacgcaa aatgccgctc atttatgagt
    16261 aaacctttca ctattattac gttttttcaa gctgggacgc gcacgacaca gagaattaac
    16321 taattgaaaa aattaaagat taattatctg ttcatcggca ttctggcact gctgctcgcg
    16381 gtcgctctct ggccatccat tccctggttt ggtaaagccg acaaccgtat cgccgccatt
    16441 caagcgcggg gagagttgcg tgtgagcacc attcatactc ccctgactta taacgaaatc
    16501 aacgggaaac cttttggcct ggattacgaa ctggcgaaac agtttgccga ttacctcggc
    16561 gtaaaactga aagtgaccgt gcggcagaat atcagccagc tgtttgacga ccttgataat
    16621 ggtaacgccg acctgctggc ggcaggactg gtctataaca gtgagcgggt aaaaaattat
    16681 cagcctggcc ctacctatta ttccgtgtca caacaactgg tttataaagt gggtcagtat
    16741 cgcccacgta cgctgggcaa cctgacggcg gagcaactca ccgttgcacc gggtcatgtg
    16801 gtggttaacg atctccagac cctgaaagaa acaaaattcc cggaattaag ctggaaggta
    16861 gacgacaaaa aaggctctgc ggaattaatg gaagatgtca tcgaaggaaa actcgattac
    16921 accattgctg attctgtcgc catcagcctg tttcagcgcg ttcacccgga gctcgccgta
    16981 gcgctcgata tcaccgatga acaaccggtg acttggttta gcccgttaga tggcgataat
    17041 accctttccg ccgccctgct cgacttcttc aacgaaatga atgaagacgg tacgctggca
    17101 cgcattgaag agaaatacct ggggcatggc gatgattttg attacgtcga tacgcgcaca
    17161 tttttacgcg ccgtcgatgc ggtactgccg cagttaaagc ccctgtttga gaaatacgcc
    17221 gaagaaattg actggcgttt gctggccgct attgcttatc aggaatcgca ctgggatgca
    17281 caggccactt caccgacggg tgtgcgcggc atgatgatgt taaccaaaaa taccgcgcaa
    17341 agcctcggca ttacggatcg taccgatgcc gaacagagca tcagcggtgg cgtgcgttat
    17401 ttgcaggata tgatgagtaa agtgccggaa agtgtgccgg agaacgaacg gatctggttt
    17461 gccctcgctg cgtacaatat gggctatgcg catatgctgg atgcccgcgc cctgacggca
    17521 aaaaccaaag ggaatcctga cagttgggct gacgtaaaac agcgtctgcc tttacttagc
    17581 cagaaaccct attacagcaa gctgacttac ggctacgctc gtgggcatga agcctacgct
    17641 tatgtcgaaa atattcgtaa gtatcagatt agcctggtgg gttatctgca agagaaagag
    17701 aagcaggcta cagaagcggc gatgcaactg gcgcaggatt atccggcggt atcgcctacg
    17761 gagttgggca aagagaaatt tccttttctc tcgtttcttt cccagtcgtc atcaaactat
    17821 ttgacccatt ctccctctct gctgttttcc aggaaaggga gtgaagagaa acaaaattaa
    17881 tccgtcgagg attgcgcttt tttctgcgct ttaatttcct ggcggcgcat gcgaaagaag
    17941 tcactgagca acgccgcgca ctcatccgcc agtattcctt ccgtaatttc cactcggtga
    18001 ttcatacccg gatgatgcag cacatccatt aaagatcccg cagcgccagt tttcgcgtca
    18061 cgcgcaccaa agaccacgcg accaatgcga ctgtggatca tcgctccggc acacattaca
    18121 catggttcaa gcgtgacata caacgtggcg tcgatcagac gataattttg catcaccaga
    18181 ccaccctgcc gcagggccat gatttctgca tgtgcggtgg gatcatggcg accaatcggg
    18241 cggttccagc cttcgccgat tacccgattg ttatgcacta ataccgcgcc gaccggcact
    18301 tcccgctcat cccaggcacg tttcgccagc gtcagcgcgt gacgcatcca gtattcgtgg
    18361 ctaaattcga cttcagacaa aaagaaaact ccggttataa aagcgcggcg cattatacac
    18421 ggacgctatg ctttactcta ttccagttgc tggagttcac cgcgcggggt tactcgccaa
    18481 cgatgctgac agaaataaag cagcggattg tcctgattac tgtcgctata gccactgtac
    18541 agccgcagcg gcgtgccgat tttgcgctcc agttgtgcga ccttttcatg tcccagacaa
    18601 cgcatcgtca atacccaacc accatagcca cgctgaattt ggctggcgat aagattaacc
    18661 cgcggcagcc agggcgtatc gaaataaacc gcttcaacca gcggctgcgg agagccggta
    18721 atcaaccaga tatcagcatc ggaacttaac aggtaggtgg ttaatcgctc ctgaaccagc
    18781 ggaaaggcgg taacattgtc gcgaaaccag cgcacgaaat cggcctgcaa cgtctgtaaa
    18841 cgtgcttcgc tgtgaccaaa agtgcacccc cacagaagca gactcatcgg ccagcgtgcc
    18901 gcacgacctt ttatcaataa cgcaatggct ataatcggta acaacggcag gacaagtaac
    18961 gcattcagcg gttggcgacg cagtaaatag cgcagaaaac tgccgaacat atcctgctga
    19021 tgcaatgttc catctaagtc aaaaaacacc acacgacgct cgtgagttgc caaaccatac
    19081 tcctctggac tgaaacatcc tgattaattt tcgctactac atagcctaac agatagatca
    19141 tcacttttcc ggcaacaatc gaccgcatca tggctaactg gaatttttaa ttcatggcaa
    19201 ttagcggcaa tggaatataa aattcactcg cgtgtgtctc atattgcctg ttgtcgccac
    19261 tatttcatcg cgaggaaaga tatgaacggc ttacttcgta tccgtcagcg ttaccagggg
    19321 cttgcccaga gtgataaaaa actggcggat tatctgctgc tacaacctga tacggcgcgc
    19381 catttaagct ctcagcaact ggccaacgaa gccggagtca gtcagtccag cgtcgtgaag
    19441 ttcgcgcaaa aactcggcta taaaggtttt ccggcgctta agttggcgtt gagtgaagcg
    19501 ctggcaagcc agccggaatc gccctccgtg cccattcata accaaatccg cggtgatgat
    19561 ccgttacggc tggtcggcga aaaactgatt aaagaaaata ctgccgctat gtacgcaacg
    19621 ctaaacgtta atagtgaaga gaaactgcat gaatgcgtaa caatgttgcg ctctgcgcgg
    19681 cggataattc tgaccggtat tggcgcttcg ggtctggtgg cgcaaaactt tgcctggaag
    19741 ctgatgaaga ttggcttcaa tgctgccgca ctgcgcgata tgcatgcgct actcgcaaca
    19801 gtacaggcgt cgtcccctga cgatctgtta ttagccattt cctacaccgg tgtacgacgc
    19861 gagttaaacc tggcggcaga tgagatgctg cgagtgggcg gaaaagtgct ggcgattacc
    19921 ggctttactc cgaatgccct gcaacagcgt gcttctcatt gcctgtatac cattgccgaa
    19981 gaacaagcga caaacagtgc ttcaatctct gcttgtcacg ctcagggaat gttaacggat
    20041 ttgctgttca ttgcgctgat tcagcaggat ctggaactgg caccagaacg tattcgtcat
    20101 agtgaagcgc tggtgaaaaa actggtctga gtaaagaatg cgcgtataat gcccgcccgg
    20161 tttgtgttgt tttgagagtt tccttatggc gctgttaatc actaaaaaat gcatcaattg
    20221 tgatatgtgt gaaatcaaat aaaaacacat ttattttcat agtgatacag caatgtgtta
    20281 ttgaatgtgt tgttggattt atggtgcaaa aaaattttgc ataagttcag caacacaaag
    20341 aacaccactt tctttaacgt cctccgcgcc ctaaaggacg gggctttacg gcgcaaaagg
    20401 cctgtgacat acaacattaa cttttcagtc aaccgtagca cgtccttgta tacgccgtgt
    20461 ctgcggtttt atataaccct ggctggaaac ctcttataca aagtcgattc gccaacatca
    20521 tagatgatcg ccaccttctg gcgagaaact cctgatgcaa ttagtcgccc ggcctgcgcc
    20581 cattattctg gtgaaagttt gggacggcga ccgccaattc gtccctgtgc gcgagcagct
    20641 tccagtcctg cttttgttcg ttcaacaatc agttcacgtt ccatttcagc cagggcaccc
    20701 atcacatgga agaaaaagcg tcccattggt gtgctggtat caattgaatc agtcagacta
    20761 cgaaagttga tgcctcgttc gcgcaactcc tccaccagca cgacaagatg ccgcatactg
    20821 cgccccagcc gatccagctt ccagacaacc agtgtgtcgc ctgccgataa tgtcctgagc
    20881 agttttttca gccccggtct ttcggacttt gtaccactta tcttgtcttc aaaaatcagc
    20941 tcgcatcctg cacagttcaa cgcattacgt tgtagatcgg tattctggtc atttgttgat
    21001 acgcgtacat agccaataag catggtaa
//
LOCUS       DAJTXY010000067        20657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTXY010000067 DAJTXY010000000
VERSION     DAJTXY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.067"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..462)
                     /locus_tag="OO566_002499"
     CDS             complement(<1..462)
                     /locus_tag="OO566_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6605744.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLV"
     gene            847..1035
                     /locus_tag="OO566_002500"
     CDS             847..1035
                     /locus_tag="OO566_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6605745.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            986..1681
                     /locus_tag="OO566_002501"
     CDS             986..1681
                     /locus_tag="OO566_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCR6605746.1"
                     /translation="MGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETISCWLARTII
                     QTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFKVQPYPK"
     gene            complement(1793..2140)
                     /locus_tag="OO566_002502"
                     /pseudo
     CDS             complement(1793..2140)
                     /locus_tag="OO566_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2109..3766)
                     /gene="dpiB"
                     /locus_tag="OO566_002503"
                     /pseudo
     CDS             complement(2109..3766)
                     /gene="dpiB"
                     /locus_tag="OO566_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4147..5205
                     /gene="citC"
                     /locus_tag="OO566_002504"
     CDS             4147..5205
                     /gene="citC"
                     /locus_tag="OO566_002504"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6605747.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5220..5516
                     /gene="citD"
                     /locus_tag="OO566_002505"
     CDS             5220..5516
                     /gene="citD"
                     /locus_tag="OO566_002505"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6605748.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5513..6421
                     /gene="citE"
                     /locus_tag="OO566_002506"
     CDS             5513..6421
                     /gene="citE"
                     /locus_tag="OO566_002506"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6605749.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACVREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6432..7964
                     /gene="citF"
                     /locus_tag="OO566_002507"
     CDS             6432..7964
                     /gene="citF"
                     /locus_tag="OO566_002507"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6605750.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            7968..8519
                     /gene="citX"
                     /locus_tag="OO566_002508"
     CDS             7968..8519
                     /gene="citX"
                     /locus_tag="OO566_002508"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR6605751.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8494..9372
                     /gene="citG"
                     /locus_tag="OO566_002509"
     CDS             8494..9372
                     /gene="citG"
                     /locus_tag="OO566_002509"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6605752.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9423..10886
                     /gene="citT"
                     /locus_tag="OO566_002510"
     CDS             9423..10886
                     /gene="citT"
                     /locus_tag="OO566_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR6605753.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11000..11806
                     /gene="rna"
                     /locus_tag="OO566_002511"
     CDS             11000..11806
                     /gene="rna"
                     /locus_tag="OO566_002511"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6605754.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12035..12445
                     /gene="rnk"
                     /locus_tag="OO566_002512"
     CDS             12035..12445
                     /gene="rnk"
                     /locus_tag="OO566_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6605755.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12676..13913)
                     /locus_tag="OO566_002513"
                     /pseudo
     CDS             complement(12676..13913)
                     /locus_tag="OO566_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            14134..14562
                     /gene="uspG"
                     /locus_tag="OO566_002514"
     CDS             14134..14562
                     /gene="uspG"
                     /locus_tag="OO566_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6605756.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14683..16248)
                     /gene="ahpF"
                     /locus_tag="OO566_002515"
     CDS             complement(14683..16248)
                     /gene="ahpF"
                     /locus_tag="OO566_002515"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6605757.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16493..17056)
                     /gene="ahpC"
                     /locus_tag="OO566_002516"
     CDS             complement(16493..17056)
                     /gene="ahpC"
                     /locus_tag="OO566_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6605758.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17428..18174
                     /gene="dsbG"
                     /locus_tag="OO566_002517"
     CDS             17428..18174
                     /gene="dsbG"
                     /locus_tag="OO566_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6605759.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKHGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18384..19286
                     /locus_tag="OO566_002518"
     CDS             18384..19286
                     /locus_tag="OO566_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6605760.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19433..19636
                     /locus_tag="OO566_002519"
                     /pseudo
     CDS             19433..19636
                     /locus_tag="OO566_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(19655..20657)
                     /locus_tag="OO566_002520"
                     /pseudo
     CDS             complement(19655..20657)
                     /locus_tag="OO566_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         5175 a         5327 c         4946 g         5209 t
ORIGIN      
        1 aaccagacca tgccccatgc ttatccggcc agttctgaga ttaagcgtaa aggggcgtag
       61 tggccctata ttaccatttt ccccttcatt ctctcgtgta gggatgatat gcaggcattc
      121 ttcagaacga cgaaaaatgg caccaaaaga tgaattaaat attctcagtg cattgaccgt
      181 cgatattttt acttcactgc tgaaaagggc tttaacaaga acatacagag catcccattt
      241 aagattcatc aggtctttaa cccggccgcc agtgaggtgg tgtctccatt tgaaatactc
      301 attgccgttg tcgcctgttt caaaccacat gtatgaatca gtatcgctgt cggcatcatt
      361 tttaaatcca atctttgccc agtcagtatt ccgaatccag gcaaggattg agtcgttttc
      421 aaaagtaagt ccaccggaca acgtatcgcc atttttttgc acggcgttac cagccttgtt
      481 taccgtttcc tgtaaaccga ggggccgttt ctggcctgca tggcatgatt tccgctttta
      541 aacggggaat tcagcgtgct gattggctat gtaagggtat caacaaatga ccagaataca
      601 gacctgcaac gaaacgctct tgtttgtgca ggatgtgaat aaatatttga agataaatta
      661 agcggaacaa ggacagaccg accgggatta aaacgtgctt taaagcgcct tcaaaaagga
      721 acgcatatag aaaacgacga tcgaatcaat taaatcgatc tgtattaccg atcgattatg
      781 tcccgataac cacacccgac tgatgagatt ttttaagata acggcgaatt gatgcaagga
      841 ggtgaaatga aatcaattcg ctgtaagaac tgcaacaaac tgttatttaa agcggattcc
      901 tttgatcaca ttgaaatcag gtgtccgcgt tgcaaacgtc acatcataat gctgaatgcc
      961 tgcgagcatc ccacggagaa acattgtggg aaaagagaag aaatcacgca ttctgacgaa
     1021 accgtgcgtt attgagtatg aaggccagat tgttggctat ggttcaaagg agctgcgcgt
     1081 tgaaaccata tcctgctggc tggcccgcac aatcattcag acaaagcact attcccgccg
     1141 ttttgtgaat aactcttacc ttcacctggg agtattcagc gggcgtgatc tggttggcgt
     1201 tctccagtgg ggatatgccc ttaaccccaa ctcaggtcgt cgtgtcgtgc ttgaaacgga
     1261 taaccggggc tatatggagt tgaatcgcat gtggctacac gacgacatgc cccgcaactc
     1321 tgaatcacgg gccatcagct acgcgctgaa agttatcaga ttactgtatc cgtcagtgga
     1381 gtgggttcag tcctttgctg acgaacgctg cggactcgca ggcgttgtgt atcaggcgtc
     1441 gaattttgat tttattggca gccatgaaag cacgttctac gagctggatg gtgagtggta
     1501 tcacgagata acgatgaacg cgattaagcg cggagggcaa cgaggcgagt atttgcgagc
     1561 taataaggag cgtgccgtgg tgcacaaatt taatcagtat cgctacatca gattcctgaa
     1621 caagcgagca aggaagcggc taaataccaa actattcaag gttcagccat accctaagtg
     1681 atccccatgt aatgaataaa aagcggtaat taatactgct gtttcatttg aagcgcgaaa
     1741 gctaaagttt tcgcatttat cgtgaaacgc tttcgcgttt ttcgtgcgcc gcttcaccca
     1801 gccgttcata ggcaattggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     1861 acaccgtttc catatcactg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     1921 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     1981 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccaggttt cccgccagta
     2041 atatctgact gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2101 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2161 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2221 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2281 cgcgtactga cccctgctca aatattttgt ctcgtagaga ctctggaacg ccgcagccct
     2341 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgctct
     2401 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2461 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2521 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2581 ccacctggcg atcggcaaac gcctcgcgca ggctgtcaat aagctgttgc tgggcctgag
     2641 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2701 gagtcgacat ccaattgaga tgctcgtgtc gcaatgtacg aaggctctca acgtattgtt
     2761 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactgcga aagctgataa
     2821 tggctcccag caaatcatca cctgaacgaa tagcttcccg attggcaata acgctcagac
     2881 cgttaaagtt agccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2941 cagacctgac cacttcagca atgggtttac ccaaccattg ccgtccaggg gaactcagcc
     3001 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accgtgcgga tccaccgcaa
     3061 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacc cgtgcgattt
     3121 gctttggctc catgcccatc atctgccgac ggatatgcgc ggccaggaac cacgacagca
     3181 acatcagaat ccctaacagc acgacaaaca cacctgccat cggtaataaa aactcagccc
     3241 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3301 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatacc cattgaccct ttcccggtaa
     3361 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3421 ttttctccgg attaggatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3481 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3541 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3601 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3661 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgtca
     3721 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3781 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3841 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcgagtc
     3901 atttcgtaat tacattaatt aaaaaactta aaaccattaa acaaataaaa aaaccactaa
     3961 ctctatgtga aataaatcaa aatttcacgc cgaaatactc cttaggatgt atagcgaaag
     4021 gagaaaaaga tatacctcga tcacccccct ttctcccaag tgaaaataaa aggttatcag
     4081 tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa catgcacctt caggatacta
     4141 tttattatgt tcgtcaatga tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg
     4201 gcggaaatcg cccaattcct gcatgaaaat gatttgagcg ttgacaccac agtcgaagta
     4261 tttattaccg taacccgcga tgaaaagctt atcgcgtgcg gtggaattgc cggaaatatt
     4321 attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag gactggcgct gacattagcc
     4381 actgaactga taaacctcgc ctatgagcgg cacagcacgc atctgtttat ttataccaaa
     4441 accgaatacg aggcgctgtt ccgccagtgc ggtttttcca cgctgacctg cgtacccggc
     4501 gtgatggtgc tgatggaaaa cagcgccacg cgactgaaac gctatgccga atcgctgaaa
     4561 aaattgcgtc atccagggaa caagattggc tgcattgtga tgaacgccaa tccctttacg
     4621 aatggtcacc gttatctgat tcaacaagct gcggcacagt gcgactggtt tcatctgttt
     4681 ttagtcaaag aagattcttc acgcttcccc tatgaagacc ggctggatct ggtgttaaaa
     4741 ggcaccgccg atattccacg cctgactgtg catcgcggct cagaatacat catctcccgc
     4801 gctacgttcc cttgctactt cattaaagaa cagagcgtca ttaaccattg ttacaccgaa
     4861 attgatctga agattttccg tcagtacctc gctcccgcgc ttggtgtaac tcaccgcttt
     4921 gtcggtactg aacccttttg tcgcgttacc gcccagtaca accaggatat gcgctactgg
     4981 ctggaaacgc cgactatctc cgcagcgccc atcgaactgg ttgaaattga gcggctgcgt
     5041 taccaggaga tgccgatatc cgcttcccgg gtacgtcaac tgctggcgaa aaacgatctc
     5101 acggctatcg cgccgctggt ccctgcagtc acgctgcatt atttgcagaa cctgcttgaa
     5161 cactcccgcc aggacgcggc agctcgtcaa aagacccccg catgagaaac aggtgaaaaa
     5221 tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc tggggatgtg atgatacgca
     5281 tcgccccact cgatacgcag gatatcgacc tgcaaatcaa tagcagcgtt gagaaacagt
     5341 ttggcgatgc aattcgcacc accattctgg acgttctcgc ccgctacaac gtgcgcggcg
     5401 tacagctgaa tgtcgatgac aaaggcgcac tggactgcat tttacgtgca cgactggaag
     5461 ccctgctggc acgcgccagc ggtatcccgg ctctgccatg ggaggattgc caatgatttc
     5521 cgcttcgctg caacaacgta aaactcgcac ccgccgcagc atgttgtttg tgcctggtgc
     5581 caatgccgcg atggtcagca actccttcat ctacccggct gatgccctga tgtttgacct
     5641 cgaagactcc gtggcattac gtgaaaaaga caccgcccgc cgcatggttt atcacgcgct
     5701 gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt gtcaacgcgc tggattccga
     5761 atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt gcggacgttg tgcgtctgcc
     5821 gaaaaccgat accgctcagg atgttctgga tattgaaaaa gagatcctgc gtatagaaaa
     5881 agcctgtgtt cgtgaacccg gcagcaccgg cctgctggcg gcgattgaat ctccgctggg
     5941 cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt ttgatcggta tcgccctcgg
     6001 tgcagaagac tatgtgcgca acctgcgtac agaacgctcc ccggaaggaa ctgaactgct
     6061 gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg ggtattcagg cgttcgatac
     6121 cgtctattcc gacgctaaca acgaagccgg attcctgcaa gaagccgccc acatcaaaca
     6181 gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag attgatctgc tgcacaacct
     6241 ctacgcaccg acccagaaag aagtggatca cgcccgccgc gtcgtagaag ccgctgaagc
     6301 cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggt aagatggtgg acggtccggt
     6361 tatcgatcgc gcccgtctgg tgctctcccg tgcagaactt tccggcatcc gcgaagaata
     6421 aggcaatcaa aatgacgcag aaaattgaac aatctcaacg acaagaacgg gtagcggcct
     6481 ggaatcgtcg cactgaatgc gatcttgccg ctttccagaa ctcaccaaag caaacctacc
     6541 aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct ggaagaagcg attcgtcgct
     6601 caggtttaca ggacggcatg acggtttcct tccatcacgc tttccgtggc ggtgacctga
     6661 ccgtcaatat ggtgatggac gtcatcgcga agatgggctt taaaaacctg accctggcgt
     6721 ccagctccct gagtgattgc catgcgccgc tggtagaaca cattcgccag ggcgtggtta
     6781 cccgcattta tacctctggc ctacgcggtc cactggcgga agagatctcc cgtggtctgc
     6841 tggcggaacc ggtgcagatc cactctcacg gcggtcgtgt gcatctggta cagagcggcg
     6901 aactgaatat cgacgtggct ttcctcggcg tcccgtcctg tgatgaattc ggtaatgcca
     6961 acggctacac cggtaaagcc tgctgcggct ccctcggcta tgcaatggtt gatgccgaca
     7021 acgcaaaaca ggtcgtgatg cttaccgaag aactgctgcc ttatccgcat aatccggcaa
     7081 gcattgagca agatcaggtt gatttgatcg tcaaagttga tcgcgttggc gatgctgcaa
     7141 aaatcggcgc tggtactacc cgtatgacca ctaacccgcg cgaactgctt attgcccgta
     7201 gcgctgcgga tgtgattgtc aactctggct acttcaaaga aggtttctcc atgcaaaccg
     7261 gcaccggcgg cgcttcgctg gcggtaaccc gtttcctgga agacaaaatg cgtagccgcg
     7321 atattcgcgc cgacttcgcc ctcggcggta ttaccgcgac gatggttgat ctgcacgaaa
     7381 aaggtctgat ccgcaaactg ctggatgtgc agagctttga cagccatgct gcgcaatcgc
     7441 tggcccgtaa ccccaatcac atcgaaatca gcgccaacca gtacgctaac tggggttcga
     7501 aaggcgcatc ggttgatcgt ctcgacgtgg tggtactgag cgcgctggaa attgacaggc
     7561 agttcaacgt taacgtgctg accggttctg acggcgtact gcgtggcgct tccggtggtc
     7621 actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt cgcgccactg gtacgcggtc
     7681 gtattccgac tctggtggat aacgtgctga cctgcatcac cccgggctcc agtgtcgata
     7741 ttctggtcac agaccacggt atcgcagtta acccggcacg tccggaacta gcagaacgtc
     7801 tgcaggaagc gggcattaaa gtggtttcca ttgagtggct gcgcgaacgt gcgcgtctgc
     7861 tgaccggtga accacagccg attgaattca ccgaccgcgt cgttgccgtt gtgcgttacc
     7921 gcgatggctc ggtgatcgat gttgtgcatc aggtgaagga ataagccatg cacctgcttc
     7981 ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct gctcgtcagc cgggatgaaa
     8041 ggcaagcacg gcaacacgcc tggctcaagc accatcctgt tccactggtc tcctttaccg
     8101 tggttgcgcc tgggccgatt aaagacagcg aggtcacacg ccgaattttt aatcatggcg
     8161 tgacagcctt gcgtgcctta gccacaaagc agggctggca aattcaggag caggctgcac
     8221 tggtttccgc cagcgggccg gagggcatgt tgagcattgc cgccccggct cgcgatctca
     8281 agctcgccac cattgagctt gaacatagtc atcctctcgg gcggttatgg gatatcgatg
     8341 tcctgacgcc cgaaggcgaa attctctccc gccgcgacta ttcactgccg cctcgccgct
     8401 gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg aaaaacccat caactgaccg
     8461 atttactcaa ccgcatggag gcactgctga acgatgtcga tgcctgcaac gtcaactaaa
     8521 accacaaagc ttgcgacgtc attaatcgat gagtacgccc tgctgggctg gcgcgccatg
     8581 ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg atcgcattaa ctgcggtgcg
     8641 cacaaagata tggcgctgga agatttccac cgcagcgcgc tggcgattca gggctggtta
     8701 ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg caccggaagc ggtactccac
     8761 ggattacgcc caattggtat ggcttgcgaa ggtgatatgt tccgcgccac tgcgggcgta
     8821 aacacgcata aaggcagcat tttttcttta gggctgctat gtgcggcaat tggccgtttg
     8881 cttcagctca accagccggt tacgccaaca accgtttgtt ctacggcggc aagtttctgc
     8941 cgtggcctga ccgatcgcga actgcgtacc aataattcac gactgacggc aggtcaacgg
     9001 ttgtaccagc agcttggcct taccggcgca cgcggtgaag ccgaagcggg ttatccactg
     9061 gtgatcaacc acgccttgcc gcattacctc actctgctgg atcaggggtt agatcctgaa
     9121 ctggcattgc tcgatacctt gctcctactg atggctacca acggcgatac caacgttgca
     9181 tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg aggcgcaaac attattgcaa
     9241 aaagggggca ttcgaacccc cgccgatctc gattatctcc ggcagttcga cagggagtgt
     9301 atcgaacgaa atctcagtcc aggcggcagt gccgacctgc tgatccttac ctggttttta
     9361 gcacagattt aattatttaa gcacttgata aatttggaaa tattaatttt cggagaaccc
     9421 gtatgtcttt agcaaaagat aatatatgga aactattggc cccactggtg gtgatgggtg
     9481 tcatgtttct tatccctgtc cccgacggta tgccgccgca ggcatggcat tacttcgctg
     9541 tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat tccggcaaca gcgatcagtt
     9601 ttattgcggt tactatttgc gttattggca gtaattacct gctctttgat gccaaagaat
     9661 tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa atggggcctg gctggttttt
     9721 ccagcaccac ggtatggctg gtatttggcg catttatttt tgcattaggg tatgaagttt
     9781 ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt catggacaaa cgcacgctga
     9841 cgttgggtta tgcgattgtc attatcgaca ttctgctggc accgtttaca ccgtccaaca
     9901 ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa cctgccgccg ttgtttaaat
     9961 cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta tttgatgtgg atgatggtca
    10021 ttagtaccag tctgagttca tccatgtttg tcaccggtgc ggcaccaaac atgctgggtc
    10081 tggagttcgt cagcaaaatt gccggtatcc agattagctg gttgcagtgg ttcctctgct
    10141 tcctgccggt tggggttatc ttgcttatca ttgcgccgtg gctttcctac gtgctgtaca
    10201 aaccggaaat cacacacagt gaagaagtgg caacctgggc gggtgatgaa ctaaaaacca
    10261 tgggtgcgct gacacgcaga gagtggacgc tgattggcct tgtattgctc agcttaggtt
    10321 tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt tggtctgctg gcagtttcgc
    10381 taatgctggc tctgcacgtt gtgccgtgga aagacattac ccgctataac agcgcatgga
    10441 acacgctggt caacctggca actctggttg tgatggctaa cggcctgact cgttctggtt
    10501 ttattgactg gttcgccggt accatgagta cgcacctgga aggattctca ccaaacgcaa
    10561 cggtgattgt actggttctg gtgttctact ttgcacacta cctgtttgcc agcctgtctg
    10621 cgcacaccgc aaccatgctg ccggttattc tggccgtcgg taaaggtatt ccgggcgtac
    10681 caatggaaca actgtgtatc ctgctggtgc tgtctatcgg tatcatgggc tgtctgacgc
    10741 cgtatgcaac cggtcctggg gtgattattt acggctgtgg ctatgtgaaa tcaaaagatt
    10801 actggcgtct tggcgcaatc ttcggggtga tttacatctc tatgttactg ttggttggct
    10861 ggccgattct cgccatgtgg aactaatacg ttcgccaaac gctgaataaa tcatcacgcc
    10921 cgccaggtaa gcccacctgg cgggcttttt tatgatttaa tagatagtat tcagtgtatg
    10981 tatgagttcc acacccatta tgaaagcatt ctgccgtaac gccgcgttgc tcgcggtttc
    11041 tctgcttccc ttatcttctg ccaacgccgt agcgttgcag gcaaaacagt atggcgattt
    11101 tgatcgctat gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg
    11161 aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt
    11221 tctgaccgta catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga
    11281 tgaacgccgc tggatgcgct tcggttgcgc tactcgccca atcccgaatc taccagaagc
    11341 acgcgccagc cgaatgtgtt catcgccgga aaccgggtta tcactggaaa cggccgctaa
    11401 actaagtgaa gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc
    11461 caaacacggt gcctgctttg gttttgatcc ggacgcatac ttcggtacga tggtacgcct
    11521 gaatcaagaa attaaagaga gcgaagccgg aaaattcctt gcggataatt acggtaaaac
    11581 agtgagccgc cgtgactttg acgccgcctt tgccaaaagc tggggaaaag agaacgtgaa
    11641 agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc agatctcgat
    11701 caaagctgac gccatcaacg ctccgctttc tgcaaactca ttcttgccac aacctcaccc
    11761 aggtaactgt ggcaaaacct ttgtgattga taaagcgggt tattaatcct ctgatttaca
    11821 aaacacgcta attcgtcttc cataaccatg ccgattaaga attatacata atgcgctgca
    11881 ttgaggaatt ccctgaatgt gacgcaaatc acttcaaaga gtgagcaaca gtcagtatca
    11941 tcatgtgagt taaaccctcg ccgcctgacg gtgagggttt tcttttgggt atgtttatct
    12001 gcgacactcg cagtaccgac gacatggagt aaaaatgtcc agaccaacta tcatcattaa
    12061 cgacctggat gccgaacgca tcgatattct gctggagcaa cccgcctatg ctggtttgcc
    12121 aatcgccgac gcgttaaacg cagagttgga tcgcgcccaa atgtgttcgc cagaagagat
    12181 gccacacgac gtggtgacaa tgaacagccg ggttaaattc cgcaatctta gagatggcga
    12241 agtgcgtgtg cgcacgctgg tgtatccggc aaaaatgacc gatagcaata ctcagctttc
    12301 cgttatggct ccggtaggtg ccgcactgct ggggctgcgc gttggcgatt ccattcactg
    12361 ggaacttccg ggcggcgttg caacccacct tgaagtgctg gaactcgaat accagccaga
    12421 agctgctggc gactacctgc tttaatccca tctgcacaag cgataactcg ctttgcccag
    12481 aggatgcact cgcatcctct ttacgcaaaa tcagcaattt ttcctggttc tcactgttca
    12541 cttttgctgc aaaattattc cgtttcgttt gtgggaataa ccatggcaga agcattttac
    12601 atcctgatag gatttctgat tatggcggcg attatcgtca tggctgtcct ttacctcgaa
    12661 aaccactcct gacgatcata ttgttccccc cggcatcgca ttcaccagac ctgaaaccgc
    12721 cttctgcgcc gcctctgcgc tttgtgcacc gggtaccaga atcaccttac ggcactcctc
    12781 ttcacgtttt tcgaaaatct catatccccg ggcggcctct tcaaacggca tatagtgggt
    12841 aacaatttct tctggtttca gtaatccttt ctcaattaac ggtaataatt ctcccagcca
    12901 tgcgtgaacg tgggtctgtc ccattttaaa cgtcaaccct ttatcaaagg cgtcgccaaa
    12961 caggaaaccg tgaataaatc cagcgtagac gcccggtacg ctaacaatgc cgccacgcct
    13021 gaccgccgca atacactggc gcaacgcttt accgctgctg ccctccagtt tcaggttagt
    13081 cagcaccgtt tccgtggtgc tgcctttcgc ttcaaaaccg acggcgtcta ttactgcatc
    13141 cacgccccgg tgacctgccg tttgttcaat aattgactgt gccggatcgc tatcttcatc
    13201 gaaattaatc gggatcgcgc cgtagcggtc agcggcgaaa cgcaagcggt agggatgatg
    13261 atcaacaaca aaaatctgtt ccgcaccgag caaccgtgca caggcgattg tcaacaatcc
    13321 cacaggacca gcaccataga ctgcaacgct tgaaccttgt tggatctgca cattttttgc
    13381 tgcctgccat gccgttggca gaatatcaga aaggaaaagg gctttatcat ctgaaagcaa
    13441 aggcggtact ttaaacggcc ccacattccc tttagggacg cggacatatt ccgcctgccc
    13501 gctaggaacg ccaccataca ggtgactata accaaacaat gccgcgggag caggtatctg
    13561 ttttttattg agcgcagcgc ctttacccgc attggtattt tcgcaggcgg catattgctg
    13621 taatcgacag aaaaaacagt cgccacaagc aatgacgaac ggaattacca cccggtcgcc
    13681 tttttgcaaa ttttttacgt ccttcccggt ttcaacgact tccccataaa ttcatgacca
    13741 aaaatatcgc catgtttaac ctggggtatt ttgcctcgat aaagatggag gtcagagcca
    13801 cagatcgccg ttaccgtaat acgcagaata atatcatctg cctgttcaat gcccggatcg
    13861 ggaacatttt ctacctgaac gtgatgtggg ccgtgataag tcaatgcttt catgcaacct
    13921 ccagagacag ttcaaaaaag agtcagtctt taagggtagc tatcacagcg ggaaaacggg
    13981 cggtaccgca ggttttggga gaattcctgg tgttcagtga gaatatatta atcacagaaa
    14041 ccgttcgtaa ggaaaaagaa ccagacaaat atcagggata aatattataa tcatggcgaa
    14101 tgatttgttc atgattaaca gggagaaagg gttatgtata aggcaatcat tatgccagtt
    14161 gatgtatttg aaatggaatt gagcgacaaa gctgttcgcc acgctgaatt cctcgcccag
    14221 gatgacggag ttattcatct acttcacgta ctacccgggt cagccagcct gagcctgcac
    14281 cgttttgccg ctgatgtgcg tcgttttgaa gagcatctgc aacatgaagc agaagaacgt
    14341 ctgcaaacga tggtcagcca cttcaccatc gatccttccc gcattaaaca acatgtccgt
    14401 tttggtagcg tgcgggatga agtcaatgag ttggcagaag aactgggggc tgatgttgta
    14461 gttatcggtt ctcgcaaccc atcgatttcg acccatctgt taggttctaa cgcctcgagc
    14521 gtgatccgcc acgccaatct gcctgtactg gtcgttcgct aacagataaa agtatttatg
    14581 ccgccctgcc tggaacatcc cggcagggct ttttaatgcc ataaaaaagc cgccaggttt
    14641 gaccccggcg gctaagcaat tgcaggtgaa tcttacttct tcttatgcag ttttggtgcg
    14701 aatcaggtag tcaaaagcac tcagagaggc tttggcacct tcgccagtgg cgatgatgat
    14761 ctgcttgtac ggaaccgtcg tacagtcacc cgctgcgaac acgcctttga cgttggtttc
    14821 acatttcgca tcaatgataa tctcgcccat gcggttacgt tcgactgcgc cttccagcca
    14881 gttggtgttc gggagcagac caatctggac aaaaataccg gccagttcga tgttgtgaat
    14941 attgccgctg acacgatctc gatattccag accaacgact ttgctgccgt cgcctttcac
    15001 ttccgtggtt tgcgcattca gaataatgtc gacgtttttc aggctgcgca gtttgtcctg
    15061 cagaacctgg tcggctttca tttctggcgc aaattccagc agcgttacgt gctcaacgat
    15121 acccgccagg tcgattgccg cttccacgcc ggagttaccg ccgccgataa ccgctacgcg
    15181 tttgccttta aacagcgggc cgtcgcagtg cgggcagtag gtcacgcctt tagtgcgata
    15241 ctgatcttcg cccggaacgt tcatgttgcg ccattttgca cccgtcgcca cgataatgct
    15301 gcgtgctttc agtaccgcgc cagaagctgt ttcaatctgg tgtagaccac cttcaaccgc
    15361 tgccgggatc agtttgctgg cgctctggct gtcgatcaca tcaacgtcgt attcatcaac
    15421 gtgaactttc agtgcgcctg ccaacttctg cccttcagtc ttcggtacag aaatgtagtt
    15481 ttcgatatca acggtatcga ggatctgacc accaaaacgt tcgcccatca gaccggtacg
    15541 gatgccttta cgtgcggagt aaattgctgc cgctgcaccc gccgggccgg aaccaacgat
    15601 taatacgtca taagcatcac gcttgttcag ctcttctgcc gcacgttttt ccgcgccagt
    15661 atcaattttg gcaacgattt cagtcaacgt catgcggccc tgaccaaact ctttcccgtt
    15721 tacgaacact gccggaacgc ccatcacgtt gcgatcggtg atttcgttct ggaaggtgcc
    15781 gccgtcaatt gcagtgtgct tgatgcgcgg gttcaatacg ctcatcaggt tcagcgcctg
    15841 caccacgtcc gggcagttat ggcaagagag cgagtaatag gtttcgaatt caaaatcacc
    15901 gtcaatatgg cgaatctgct ccagcagaga ctgcgcttct ttcgacggat gaccaccggt
    15961 ccacagcaac gccagtacca gcgaggtgaa ctcgtggccc agcggggagc ctgcaaaacg
    16021 tggtccctgg ttggaacctg ggttggtgat caggaaagac ggcttacgca ctggcaagct
    16081 gttatcttct ttaaaggtga ctttgtctga cagttctgcg atttcagcca acagttcctt
    16141 gatttctgcc gatttagcgc tgtcatccag cgtggcaatt aactcaacag gcttggtcaa
    16201 tttctcaagg taagccttga gttgagtttt catatttgtg tcgagcatgt ttatctcctg
    16261 ggctttaaac atcatcatgc aagcggcctc tgcggctacc tggatgcagc ttgcatcatg
    16321 gtgcggggaa gaaaatcggg tgccgcagca cccgaagaat taggtgaatt tccgaagtat
    16381 ttcgcgtcag agcaaggcga ctagcctgtg aatccccggg agcttacaca agtaagtgac
    16441 cggggtgagc gggcgacgcc aacgccgcta tggcgtgaaa gacgaaggaa atttagattt
    16501 taccaaccag gtccagagac ggagccagag ttgcttcacc ttctttccat ttagccgggc
    16561 aaacttcacc tgggtgagaa gctacgtact gagctgcttt gattttacgc agcagatcag
    16621 acgcgtcacg gccaatgcct tcagcggtaa cttcgattgc ctggatgata ccctgcgggt
    16681 caacaacgaa ggtcgcacgg tcagccagac cttcatcttc acgcatgttg tcgaagttac
    16741 gggtcagggc gccagtcggg tcgccgatca tcgcatattt gattttagcg atggtttcag
    16801 agctgctgtg ccatgctttg tgggtgaagt gagtatcggt agatactgcg tatacgtcta
    16861 cgcccagttt ctgcagttct tcgtagtggt cagcaacgtc acccagttcg gtcgggcata
    16921 cgaaagtaaa gtcagccggg tagaagaaga agacgctcca gcggccttcg gtatcttttt
    16981 cggtgatttc gatgaattcg ccgtttttga atgcctggtt tttaaaaggt ttaattttgg
    17041 tgttaatcaa ggacatctat acttcctccg tgttttcgat gagatgtaag gtaaccaatt
    17101 tttgccgatt cggctaatgc gtttccatta tcaaatcgat aagttttacc ttacaacctt
    17161 cgtaagacaa ctttgtgaac tttgcaccgc cagcggcgat aaaaagtaaa atggccccct
    17221 ttccaggtga ccgtgtaatc tgaactaccc tttggtatct tcagcgcctg cctgcaggct
    17281 gaggtgttgc ccttacaaat gcaacaacga catggattac aacaccctca taaacaaagg
    17341 gcaatcacct gatctaagct cttacctatg acagtgatag gttatgcctt ttactcgact
    17401 tttgcactga ctgaaaagaa caaattaatg ttaaaaaaga tacttttact ggctctgctt
    17461 cctgcaatcg ccttcgcaga ggaacttcct tccccagtaa aagcgattga aaaacatggc
    17521 attacaatca tcaaaacgtt cgatgccccc ggaggaatga aaggttatct cggaaagtat
    17581 caggatatgg gcgtcaccat ctacctgact cccgatggta agcacgctat ctctggttac
    17641 atgtacaacg agaaaggtga aaacctgagt aacacactta tcgaaaaaga aatttacgca
    17701 ccagccggac gcgaaatgtg gcaacggatg gaacaatccc actggctcct cgacggtaaa
    17761 aaagatgcgc cggtcattgt ctacgtcttc gccgatccgt tctgcccata ttgtaaacag
    17821 ttctggcagc aggcgcgccc gtgggtagat tctggcaaag tgcaattaag aacattgttg
    17881 gttggggtta tcaagccaga aagcccggcg acagcagcgg caattcttgc ctccaaagat
    17941 cccgcaaaaa cctggcaaca atatgaagcc tctggttgca agcttaagct aaacgtgcct
    18001 gcaaacgtaa gtacagagca aatgaaagtg ttaagtgaca atgagaaact gatggacgat
    18061 ctgggggcaa atgtcacgcc ggctatctat tacatgagta aggaaaatac gctgcaacag
    18121 gccgtggggt tgcccgatca gaaaacgctt aatatcatta tgggaaataa ataaaaggta
    18181 attcatggat ggttgaacta tatccctgct tttcaaccat cctaaaaaag catgaattgc
    18241 tcgctctacc ctaagtggct gtgctatttt agaaaaactt aatcctgagt taagcaaaaa
    18301 aacataatca ataaaaatac atacgcaaaa aacattgatt aagtgaatat accatggaag
    18361 aaaaatataa accggagtag tgtatggcca atctctacga cttgaaaaag ttcgacttaa
    18421 atcttcttgt catatttgag tgtatttacc agcatttaag cattagcaaa gctgctgaat
    18481 cactatatat caccccatca gccgtgagcc agtcgttaca acgcttacgt gcgcaattta
    18541 atgacccttt attcattcgc tcaggaaaag gtatcgcgcc gacgacaaca gggttgaatc
    18601 tgcatcacca tcttgagaaa aacctcaaag gccttgaaca aacaattaat atagtcaata
    18661 agtccgaact aaagaaaaac tttattatct acggccctca gctcatttct tgctctaata
    18721 atagtatgct gatccgctgc ctacggcaag atgcctctgt cgagattgag tgccatgata
    18781 tcctcatgtc agcagagaat gctgaagaac tgttggttca ccgcaaagcc gatctggtga
    18841 tcacccaaat gccagtgatc agccgttccg tcatttgcat gccattgcat acaattcgca
    18901 atactctaat ttgtagtaat aagcatccgc gaattactga caatagcact tatgaacaaa
    18961 ttatggcaga agagttcacc caattaatat ccaaatcagc aggagttgat gatattcaaa
    19021 tggaaataga cgagaagttt atgaatcgca aaatttcatt tcgtggttcc tctttgctga
    19081 caataattaa tagcattgca gttacagatt tactgggaat agtcccctat gaactttata
    19141 actaccaccg tgactttctt aatttaaaag agataaaact agaacatccg ttaccttcaa
    19201 taaagcttta catttcctat aataaatctt ctttaaataa tttggtgttc tcccgattta
    19261 ttgaccgtct aaatgagagt ttttgatata taactacaga cagctactat aactttcatc
    19321 tatttattca cagctcgaat gaaaaataaa acactctgct ttacagtgtt tactttataa
    19381 ttaacagtct tatctcactg ttcaccctgt aacgttcaga gagcgtcttc agatgtctat
    19441 ttataaaatt cctcttccac tcaatattct ggaagctgca cgagagcgta ttacctagac
    19501 actcaacacc ctaccccgcg tttgtgtctc attttctggc ggcaaagact ctggtttaat
    19561 gctgcatctg acagccgaac ttgcccgaca aatgggcaaa aaaatctgcg ttttgtttat
    19621 cgactgggag gcgcaatgaa tcgccacgga taatctagac acttccgagc cgttgataat
    19681 actggttttc atattctgtc ggtgacatct gttcgctaga accatgccga cgcttactgt
    19741 tataaaacat ttcgatgtaa tcaaaaatat cactgcgggc ttcttcccgc gttccgtaga
    19801 tctttttctt tatccgttca cgtttcaaca actggaaaaa actttctgca accgcattat
    19861 catggcagtt accgcgacgg ctcatgctac cctccaggcc gtgtgatttc aggaacgact
    19921 gccactcatg gcttgtgtac tgactgccct gatccgaatg aaccagcacc tgtttttcgg
    19981 gattacgccg ccatacagcc atcagcagtg cgttcaggac aatgtccttt gtcatccggg
    20041 attgcatgga ccagccgata attttgcgtg agaacagatc aacaacaacg gcaagataca
    20101 gccagccttc gtgggtcctg atgtaggtta tgtccgttac ccaacgctca tcaggagcat
    20161 ccggattgaa ctgtcgctgg agcctgttgg gtgacacgat actggcctcg cctttacgtg
    20221 cccgcgggct tcggtatccg acctgagcct ttattccgac acgtttcatc agtctccaga
    20281 ctctgtttac tccgcactgt tgcccgctgt cacgcagatc cagatggatt ttgcgataac
    20341 catagacgca tcccgattcc agccagaact gtttaatctg tcctgtcagt ctcaggtctg
    20401 cctgatggcg ttgtgaatgc ggctgctgaa gccaggcgta aaaaccactg ggatgaacat
    20461 ccagcacccg acagagcagg cgaacaggcc agcaacagga gttgtcacgg ataaaggcgt
    20521 acctcagtcg gacagctttg cgaagtacgc cgcggctttt tttaatatgt cccgttcgtc
    20581 ggtaacccgt ttcagctctt tctggagacg gcggatctcg gcctgagcat ctgactgttc
    20641 tttattagtg gaagaat
//
LOCUS       DAJTXY010000068        20608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTXY010000068 DAJTXY010000000
VERSION     DAJTXY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.068"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            146..298
                     /gene="hokA"
                     /locus_tag="OO566_002521"
     CDS             146..298
                     /gene="hokA"
                     /locus_tag="OO566_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6605761.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(485..697)
                     /gene="cspA"
                     /locus_tag="OO566_002522"
     CDS             complement(485..697)
                     /gene="cspA"
                     /locus_tag="OO566_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6605762.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(978..1268)
                     /locus_tag="OO566_002523"
     CDS             complement(978..1268)
                     /locus_tag="OO566_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR6605763.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1702..2412
                     /locus_tag="OO566_002524"
     CDS             1702..2412
                     /locus_tag="OO566_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6605764.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2462..3436)
                     /gene="ghrB"
                     /locus_tag="OO566_002525"
     CDS             complement(2462..3436)
                     /gene="ghrB"
                     /locus_tag="OO566_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6605765.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3540..4199)
                     /locus_tag="OO566_002526"
     CDS             complement(3540..4199)
                     /locus_tag="OO566_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6605766.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGVLSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4352..6685
                     /gene="bisC"
                     /locus_tag="OO566_002527"
     CDS             4352..6685
                     /gene="bisC"
                     /locus_tag="OO566_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6605767.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6654..7094)
                     /locus_tag="OO566_002528"
     CDS             complement(6654..7094)
                     /locus_tag="OO566_002528"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6605768.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7091..7654)
                     /gene="tag"
                     /locus_tag="OO566_002529"
     CDS             complement(7091..7654)
                     /gene="tag"
                     /locus_tag="OO566_002529"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6605769.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7812..8510
                     /locus_tag="OO566_002530"
     CDS             7812..8510
                     /locus_tag="OO566_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6605770.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8739..9947
                     /locus_tag="OO566_002531"
     CDS             8739..9947
                     /locus_tag="OO566_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6605771.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10271..11962
                     /gene="eptB"
                     /locus_tag="OO566_002532"
     CDS             10271..11962
                     /gene="eptB"
                     /locus_tag="OO566_002532"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6605772.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASAMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12054..12130
                     /locus_tag="OO566_002533"
     tRNA            12054..12130
                     /locus_tag="OO566_002533"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12088..12090,aa:Pro,seq:cgg)
     gene            12874..14481
                     /gene="dppA"
                     /locus_tag="OO566_002534"
     CDS             12874..14481
                     /gene="dppA"
                     /locus_tag="OO566_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6605773.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FESVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14789..15808
                     /gene="dppB"
                     /locus_tag="OO566_002535"
     CDS             14789..15808
                     /gene="dppB"
                     /locus_tag="OO566_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6605774.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15818..16720
                     /gene="dppC"
                     /locus_tag="OO566_002536"
     CDS             15818..16720
                     /gene="dppC"
                     /locus_tag="OO566_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6605775.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16731..17714
                     /gene="dppD"
                     /locus_tag="OO566_002537"
     CDS             16731..17714
                     /gene="dppD"
                     /locus_tag="OO566_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6605776.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17711..18715
                     /gene="dppF"
                     /locus_tag="OO566_002538"
     CDS             17711..18715
                     /gene="dppF"
                     /locus_tag="OO566_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6605777.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18745..20016)
                     /locus_tag="OO566_002539"
     CDS             complement(18745..20016)
                     /locus_tag="OO566_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6605778.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20492..20599
                     /locus_tag="OO566_002540"
     CDS             20492..20599
                     /locus_tag="OO566_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6605779.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4931 a         5101 c         5422 g         5154 t
ORIGIN      
        1 ctccaattga atatcggaat cagacctata tgcctcgtgt ttaactgtcc aactttttgg
       61 ggtcagtaca aagtgaggct accctatgcc tgaacaccat aaggtgtgcc tcttactcgt
      121 tggaaatcaa cacagggggc tgggaatgcc gcagaaatat agattacttt ctttaatagt
      181 gatttgtttc acgcttttat ttttcacctg gatgataaga gattcactgt gtgaattgca
      241 tattaaacag gagagttatg agctggcggc gtttttagcc tacaaattga aagagtaaga
      301 gtcttcggcg ggaaattatt cccgccttac ttacggcgtt gcgcattctc attgcaccca
      361 catttattct tcacaaaaat aataatagat tttattacgc gatcgattat ttatttcctg
      421 aaaaaaaata aaaaaatccc cgcaaaatgg cagggatctt agattctgtg cttttaagca
      481 gagattacag gctggttacg ttaccagctg ccgggccttt agcgccgctt tcgatggtga
      541 aggacacttt ctgaccttcg tccagagatt tgtaaccata gttctggata gcagagaagt
      601 gtacgaacac atctttagag ccatcgtcag gagtgatgaa gccgaagcct ttgtcagcgt
      661 tgaaccattt tacgatacca gtcattttac cggacatagt gtattacctt taataattaa
      721 gtgtgccttt cggcgatatg gcgtgcttta cagattttga agcgttaaag gaatgtgcac
      781 tacgaggggt atcaacgata actcttgaag ggacttgcct tactacactg ctttaatggt
      841 ctgtacgtca aaccgttgat gtgcattaag ccactcattg gcgggtgatg caacaattat
      901 ttttcatatt tatgattaat cggttggaaa acggtcctgt catcaggacc gtaaacagca
      961 ataaagtgga taaaagtcta tttcatcaac tgcttactta atgccgggtt ggcttgaatc
     1021 aaacgcatca attttagttc ggcacttgaa ggcttcacgc gtctggattc ccattccttt
     1081 accatagcga ctgatacgcc caaaacccgg gcgaaatcat cgatttttaa tcctgtccct
     1141 tttcgtaacc gctcaatttc ggtacaggac gttgttctgt gcgtcagggt aattttctgc
     1201 gtttcatctt taaaaacaat ctgttccagg ctgctcaaca gctcatgcat tggatcttta
     1261 tattccattg aaaactcctc aaatcacact gcgggatcgt gaattgcatc gaagctcatt
     1321 aagaatagtc gggaaaagca gacacagcgg ggttaagtca gggcatgctc tggctatgat
     1381 tcgattcagc ctgtcattta gcgtgactta tagccaccta acgatctgat aaatgccgat
     1441 atatctctta tcactcctgt tttgtaaaga tttcgcaaat ctaaccttct gcgaaggcgt
     1501 aaatctctgg ctacaaaaaa ggcaacggca ctatcatttt ttaacaatga aacaacaaat
     1561 taaccttgtt tcattttctc catgatttta ccaggaagat tacgtagaaa gggtatcctg
     1621 caagcctgtc ctggactatc cttgtcacgt cagacacgcg tgtgtcgttg tgcgcttttt
     1681 ttgggtgaaa ggagtaagaa aatggcgaca ggaaagtcct gctctcgctg gtttgcgcct
     1741 cttgcggcgt tattaatggt agttagcctg agtgggtgtt ttgataaaga aggcgatcag
     1801 cgtaaagcgt ttatcgactt cctgcagaat acagtgatgc gtagcggtga acgtctacca
     1861 accctgactg ccgatcagaa aaaacagttt ggtccttttg tctctgatta cgcgattctg
     1921 tatggttatt ctcagcaggt gaatcaggcg atggattccg gtctgcgccc ggtcgtagac
     1981 agcgttaacg caattcgcgt accgcaggat tacgttacgc aaagcggtcc gctgcgtgaa
     2041 atgaatggtt cgctgggtgt gctggcgcaa caactgcaaa atgcgaagtt gcaggccgat
     2101 gctgcgcact ctgcgctaaa acagagcgat gacctgaaac cggtatttga tcaggcattc
     2161 accaaagtgg taacgacgcc agctgatgca ttgcaaccgt tgattccggc ggcacaaact
     2221 ttcacgcaac aactggtgat ggttggagac tacattgctc agcaaggtac tcaggtgagc
     2281 tttgtcgcaa atggcatcca gttcccgact tcacagcagg caagtgaata taacaaactg
     2341 attgcgccat taccggcaca acatcaggcg tttaatcagg cctggactac agcagttact
     2401 gcaacccaat aaagagtaaa agcccgagcg attatcgggc tttacttcac gcagtcgcgg
     2461 cttagtccgc gacgtgagga ttcacacagt tcttctcaac ctttccttgt aacgcatcaa
     2521 tcaaattatc cacggcacag gcggccatgc cataacgcgt ctcatgggtg gcagatccaa
     2581 tatgcggtac tgcgacgacg ttggccattg agagcaacgg cgaatctacg gacagtggct
     2641 cttgttcgaa gacatccagc ccggcggcgt gaatttcgcc tttctgcaat gctgcgatca
     2701 gtgcattttc gtcaaccacc gggccacgtc cggcattaat gaaaatggcg gaggatttca
     2761 ttttggcgaa ttgttctgcg ccaaacagat gatgcgtctc atcagttaac ggcaggatca
     2821 ggcaaacgaa atctgactct tgtaacagag tatccaaatc gcagtagcgg gcgttgaagc
     2881 gttcttctgc ttctttatgg tggcggcgcg cgttatagag gatgggcatg ttgaagccaa
     2941 agtgcgcacg ttgtgccagc gccatgccga tccgtcccat cccgacaatg cccagtgttt
     3001 tatggtgaac gtcagtgccg taccagtccg ggcctatgct cgcggtccat tcgcctgctt
     3061 ttacccgttc tgctacctcc acaacccgac gagcggtaga caacaccagc gccatcagcg
     3121 tatcggcgac ggtttctgtt aatacggttg gcgtgtgcat cagcagaatt tttcgggcgg
     3181 taagcgcatc gacatcaaaa ttgtcatagc cgacggagat cgttgatgtg gcacgcagtt
     3241 tcggcatttt ttccagcaat gcggcatcta cattctcgtt tgaacccagt aaaccttcag
     3301 cttctgcaaa aattgctgca ttttgaacga cggtttgtgg gctgaggttt gccacctggt
     3361 gaacggtgaa atgcgcttgc aggcgttgca gtaaatcatc aggtaaggct ttgtagagga
     3421 taacggacgg cttcatgctt ctctccattc actgataagg cgatcacctt agcctgaaat
     3481 atgatgaagg aaaactgaag aatgccaggt gatttctgca tcacctggca tgaaagggat
     3541 tacagcgggc ttaaggtaat ttctacacgg cggttttgcg ccttaccttc tgcggtgctg
     3601 ttgctggcga ttgggttagc cgggccaagg ccctgagtac ggatgcggct ggcgtccacg
     3661 ccctgggtga tcaacgcgct ggcaacggaa tccgcacgct gctgggagag acgcatgttc
     3721 aggtcgtgac cccccgtgct gtcggtataa ccaatcacgt taaccgccgt tttcggatac
     3781 tctttcagca ccattgccac gccggtcagg gtgttagcgc ctgccggttt caggggcgcg
     3841 ctgctgctgt cgaaggtcac attgttcggc atattgagga taatgttatc cccgctgcgg
     3901 gttacgctaa caccagtgcc gcgcattttg tcacgcagct tcgcttcctg cacatccatg
     3961 taataaccca cgccgccgcc cagagctgcg cctgctgctg cgccaatcag cgcgccttta
     4021 ccgcgatctt tcttcgaaga agagagcaca ccaatacccg cgcccacgag agagcccaga
     4081 cctgcgccga tagcagattt gcctgcttcg cgttcgccgg tgtaagggtt agttgtgcag
     4141 ccagataccg ccagagcgcc actcactacg gcggcaataa gataaacacg tttcttcatt
     4201 gttaatcctt aataaccttt ttattctttg ccacgggttc cgtggcggga gattatgccg
     4261 cgtgaacatg aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct
     4321 caatacatct aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt
     4381 tctgactgcc gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag
     4441 ctcgcgtggc gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca
     4501 ggttcacagc aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc
     4561 ggaaaacccg caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc
     4621 gctggatctt attcaccaac aacataaacg cattcgtgag gcttatggtc cggcatcgat
     4681 ttttgctggt tcctacggct ggcgttcaaa cggcgtgctg cataaggcct cgacattatt
     4741 acaacgctat atggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg
     4801 tgcggcacag gcgatcatgc cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac
     4861 cagttggccg ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg ctaacccact
     4921 caatacgctg aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttccgc
     4981 actgcgtgac agtgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga
     5041 tttctttggc gataaaatgg agtgggtggc accgcacatg ggcaccgatg ttgcgctgat
     5101 gctggggatc gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgca
     5161 ttgcaccaca ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc
     5221 gaaaaacgcc gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa tccgcgagct
     5281 ggcggctatt ttccaccaaa ataccaccat gctgatggca ggctggggaa tgcagcgcca
     5341 acagtttggt gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat
     5401 cggcacaccc ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtaaccccac
     5461 gcggcgttct gcggtgctct cttccatgca gggcagcttg ccgggtggct gcgatgcggt
     5521 ggataaaatc cctgttgccc gcattgttga agcactggaa aaccctggtg gcgcatatca
     5581 acacaacggt atgaaccgac atttcccgga tattcgtttt atctggtggg cgggcggtgc
     5641 caactttact catcatcagg ataccaatcg tctgatccgt gcctggcaaa aaccggagct
     5701 ggtggtgatc tctgaatgct tctggacggc ggcggcgaaa cacgcggata tcgttctgcc
     5761 tgcgactacc tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca
     5821 tctggtgccg atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt
     5881 ttttgccgag ttgagtgaac gttgggagaa gggcggctat gcgcggttta cggaaggaaa
     5941 aagtcagctg caatggctgg aaacgtttta taacgttgcc cggcagcgcg gggcaagcca
     6001 gcaggttgaa ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc
     6061 ggaaaacccg gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgctggc
     6121 gcatccgtta aaaaccgcca gcggcaagat tgaaatcttc tcacagcgta ttgccgatta
     6181 cggttacccg gattgccctg ggcatccaat gtggctggag ccggacgaat ggcagggcaa
     6241 tgccgaacca gaacagttgc aggtactttc tgcccatccg gcgcaccgtc tgcacagcca
     6301 gctgaattac agttctctgc gcgaattgta cgcggtagca aaccgtgagc ctgtcaccat
     6361 tcatcctgac gatgctcagg cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa
     6421 ctcccgtggg cagatccttg ctggagcggt cattagcgag ggaattaaac ctggcgtgat
     6481 ttgcattcac gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa
     6541 cggcgcagtg aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc
     6601 gggtaatacg gcgctggcat ggctggaaaa atacaacggt ccggaactga cacttacagc
     6661 gtttgaacca ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg
     6721 caatcgacaa tgtgaaaacc ctgcgcacgg taaaaatcta tcgccggttg atttttttga
     6781 taaacctcca gcatcagatg gggatagcgc tgctgcacat actgcatcag cgccttacca
     6841 ataccgcgtc tgacggcctt cggtgcgaca aacattgctg ccagaaatcg gccttccata
     6901 atgctgacaa aaccgagaag cttaccgtcc tcttcccaga cccagttttg cgcgttggca
     6961 agataggcat cccgcaccag cggaatgcag tcacgccagt aattcgcttt tataaaggga
     7021 tgcccccagg ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc
     7081 gcttcccgaa tcatggttta tttcccggat agcaacagca gccaaccaca tgatcattca
     7141 ccagcccaca tgcctgcata aaggagtaac agattgtggt gccgacaaac ttaaaaccac
     7201 attttttcag tgccttagat agggcgtcgg aggcggacgt agatgtggga atttcgctca
     7261 acgttgtggc ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcgggaa
     7321 acggttcgcc gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa
     7381 ttttccctcg atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct
     7441 gcattgctgc gaccttcacc ggatcgaact gatgaaagca ggcgcgatag ttttcgcgtt
     7501 ttttgaggac ggtgatccac gataacccag cctgctgccc ttcaaggcag atcatttcga
     7561 acagtttttt actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa
     7621 gcgggccctg actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg
     7681 taaaaatttc agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc
     7741 tactatgccc gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg
     7801 gagtcgtgtt aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga
     7861 gcgtggtaat cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg
     7921 ttgatacgca acccggacat tccacagagc gttacacctg ggatagtgat catcaacctg
     7981 attacaacga tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag
     8041 tcaatctggc ggaagaaact cctgtggatg tgaccagcag tatgagtatg ggctggaatt
     8101 ttcctttata tgaacaggtt acaaccggcc cggtcgcggc attacattac gatggcacaa
     8161 ccacctcgat gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc
     8221 atgccagcgt gagtagctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct
     8281 gggcgcaaat cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc
     8341 tgagccggat gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg
     8401 atatgttgct caatcaaaat attgccgcct atgctgcgct aactcaggca gaaaatacca
     8461 ctaataatag cgactatctg tatacgatgg gggttagcgc cagattttaa cgtaacagtc
     8521 acaattgaaa ccattgaata acaacagttg tggcgatagt gggtgctaac ttaccaaata
     8581 ataaatttgg tgaataattg tcgcgtcatt cattcctgaa ctaaggcatt tcattccgtt
     8641 ctgatggcat ttcatgccgt ttttccccaa gcataaaatg cacttcgtta tggttgtcgg
     8701 cagagatttt tcctttttat tactgcagga atactgccat gacaccttca aattatcagc
     8761 gtacccgctg gctgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt
     8821 atacctggag cctgtttaat ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg
     8881 tcgctttctc tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca
     8941 aattgcagga acgttttggc gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat
     9001 taggcttctt cctgaccgcg cattccaaca acctgatgat gctgtggtta agcgccggtg
     9061 tgctggtcgg tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt
     9121 ggttcccgga gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta
     9181 gcctgggttt caaatttatc gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct
     9241 ttgtgatttg gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag
     9301 acgcaccaaa acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg
     9361 cagagtcgat gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca
     9421 tgagcggcct gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg
     9481 atgtggtttc cgcagccaat gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc
     9541 tggtgctggg cattctgtct gacaaaattg cccgtatccg tgttatcacc attggtcagg
     9601 tgatttcgct ggtgggtatg gcggccctgc tgtttgcacc attaaatgca gtgacgttct
     9661 ttgcagcgat tgcctgcgtg gcatttaact ttggcggcac tattaccgtc tttccatcac
     9721 tggtcagtga gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg
     9781 gtttcggtat cggtagcatt tttggttcga ttatcgcctc actgtttggc ggtttctatg
     9841 tgaccttcta cgtgattttc gccctgctga ttctgtcatt ggcgctttct acgacgattc
     9901 gtcagccaga gcagaaaatg ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga
     9961 gctactgggc ttcagcttcc gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac
    10021 tcgtaaaatt tgggccagtt aactggccct ttttactgct ttttgtaacg ctaaagtctc
    10081 ttttcaaact tgcatttttg taaatttgtg cttcatgcac actctttccc cacacttttt
    10141 cgctttgctg tggtctactt attcgcgcgt gtagatttta cttatctgac tacctccgca
    10201 cttttcccct gccgggcctg aaaagccgct aagcagggtg ttatcacctg tttgtccagg
    10261 gtttgtttgc atgagataca tcaaatcgat tacacagcag aagctgagct ttttgcttgc
    10321 aatctatatt ggccttttta tgaatggcgc ggttttttat cgccgcttcg gcagctatgc
    10381 gcacgatttt accgtctgga aaggcgtttc tgctgttgtt gaactggccg ccaccgtact
    10441 ggtgaccttc tttttactac gtcttctatc gctgtttggc cgccgcagct ggcgtattct
    10501 ggcgtcgctg gtggtgctct tttccgcagg tgccagctat tacatgacct tccttaatgt
    10561 ggtcattggt tatggcatca tcgcttccgc catgaccacc gatatcgacc tgtcaaaaga
    10621 agttgttggt ctgaacttta ttctctggtt aatcgccgtt agtgcattgc ctcttatcct
    10681 tatctggaat aaccgctgtc gctacacctt gctccgacaa ctgcgaaccc cagggcagcg
    10741 tattcgcagc ctggtggtcg tcgtactggc gggtattatg gtttgggcac cgattcgttt
    10801 gctggatatc cagcagaaga aagtggagag ggcgaccggc gttgatttgc cgagttatgg
    10861 cggtgtcgta gcgaactctt atctgccatc aaactggctt tctgcgttgg ggctgtatgc
    10921 ctgggcgcgg gtcgatgaat cttccgataa taattcattg cttaatccgg cgaagaagtt
    10981 tacataccag gctccacaaa acgttgatga tacttatgtc gtgtttatca tcggtgaaac
    11041 cacgcgttgg gaccatatgg gtattttcgg ctatgagcgt aataccacgc cgaaactggc
    11101 ccaggagaaa aatctggcgg cgttccgtgg ttactcctgt gataccgcaa ccaaactctc
    11161 actgcgttgc atgtttgtac gtcagggggg cgcggaagat aatccgcagc gcacattaaa
    11221 agaacagaac attttcgcgg ttctgaagca gttaggattc agttctgacc tctacgctat
    11281 gcagagcgaa atgtggttct acagcaacac gatggcagac aacattgctt atcgtgagca
    11341 gattggtgcg gagccacgta atcgtggcaa gccggtagat gatatgttgc tggtagacga
    11401 aatgcagcaa tcgctagggc gcaacccgga tggtaagcat ctgatcattc tgcataccaa
    11461 aggttcgcat tttaactaca cccagcgtta tccgcgtagc ttcgcgcagt ggaagccgga
    11521 atgtattggt gttgatagcg gctgtaccaa agcgcagatg atcaactcct atgacaactc
    11581 ggtgacctat gtggatcact ttatctccag cgtgattgat caggttcgcg ataagaaagc
    11641 gattgtgttc tacgcagctg accacggtga gtcaattaat gaacgcgagc acctgcacgg
    11701 cacgccgcgt gaactggcac cgccggagca gttccgcgta ccgatgatgg tctggatgtc
    11761 agataaatat ctggaaaatc cggccaatgc gcaggcgttt gcgcagctga aaaaagaagc
    11821 cgacatgaaa gtgccacgcc gtcacgtaga gctgtacgac accatcatgg gttgtcttgg
    11881 ctatacttca ccggatggtg gaattaacga aaacaacaac tggtgtcaca tcccgcagac
    11941 aaaagtggca gcggctaact aagcggcctg ctgactttct cgccgatcaa aaggcatttt
    12001 gctattaagg gattgacgag ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat
    12061 tggcgcagcc tggtagcgca cttcgttcgg gacgaagggg tcggaggttc gaatcctcta
    12121 tcaccgacca aattcgaaaa gcctgctcag cgagcaggtt tttttgcatt tgtagctcat
    12181 gaggatgaga acctccgggg gcagggaggc tcgactcgac gtaggcctga taagacgcgc
    12241 cagcgtcgca tcaggcaacg gcaccgcact tattgtcgga tgcggcgcga ccgccttatt
    12301 cgaccaacac accacacatt ttcctgccaa ctttgcgccc catatctcac tatccattaa
    12361 cggatttgtg acagaaacca ttgtcttttt ttatatttgc ttaaatcttt tttgcgttac
    12421 ttatggctgg cgctggcatt ttccgctgtg cgttttagcg ttcatggtat taatcgctca
    12481 gatatccacc ttttcctcag atttttttca ttaaatgcat aaaacttaat caccaaattt
    12541 tgctaaatat taagctgatt gttctggtta tgttggagta gcacaaattt ctctgctgca
    12601 aatagctgtt tgaagttttt ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc
    12661 cggttggctg tattgacgtt ttcacattct gttgacagat tgtaggtcac gaggggcatt
    12721 ttatggagga tccgcactgt tacactgatg ttaattagta cggcatcccc acctcataac
    12781 gttgacccga ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt
    12841 taaaacaaca aacatcacaa ttggagcaga ataatgcgta tttccttgaa aaagtcaggg
    12901 atgctgaagc ttggtctcag cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa
    12961 actctggttt attgctcaga aggatctccg gaagggttta acccgcagct gtttacctcc
    13021 ggcaccacct atgacgcctc ttccgtcccg ctttataacc gcctggttga atttaaaatc
    13081 ggcaccaccg aagtgatccc gggcctcgct gaaaagtggg aagtcagcga agacggtaaa
    13141 acctatacct tccatctgcg taaaggtgtg aagtggcacg acaataaaga attcaaaccg
    13201 acgcgcgaaa tgaacgccga tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa
    13261 aacccgtacc ataaagtttc tggcggcagc tacgaatact tcgaaggtat gggcttgccg
    13321 gagctgatca gcgaagtgaa aaaggtggac gataacaccg ttcagtttgt gctgactcga
    13381 ccggaagcgc cgttcctcgc tgacctggca atggacttcg cctctattct gtcaaaagaa
    13441 tatgctgacg cgatgatgaa agccggtaca ccggaaaaac tggacctcaa cccaatcgga
    13501 accggtccgt tccagttaca gcagtaccaa aaagattccc gtatccgcta caaagcgttt
    13561 gatggctact ggggcaccaa accgcagatc gatacgctgg tcttctctat tacccctgat
    13621 gcttccgtgc gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc gtacccgaac
    13681 ccggcagaca tcgctcgcat gaagcaggat aaatccatca acctgatgga aatgccgggg
    13741 ctgaacgtcg gctacctctc gtataacgtg cagaaaaaac cgctggatga cgtgaaagtt
    13801 cgccaggcac tgacctacgc ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc
    13861 gcgggtgtat cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa cgacgacgtt
    13921 caggattaca cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc gggcctggaa
    13981 aaaggtttct ccatcgacct gtgggcgatg ccggtacaac gtccgtataa cccgaacgct
    14041 cgccgcatgg cggagatgat tcaggcagac tgggcaaaag tgggcgtgca ggccaaaatc
    14101 gtcacctacg aatggggtga gtacctcaag cgtgcgaaag atggcgaaca tcagacggta
    14161 atgatgggct ggactggcga taacggggac ccggataact tcttcgccac cctgttcagc
    14221 tgcgccgcct ctgaacaagg ttctaactac tcaaaatggt gctacaaacc gtttgaagat
    14281 ctgattcaac cggcgcgtgc taccgacgac cataacaaac gcgttgaact gtacaagcaa
    14341 gcgcaggtgg tgatgcacga tcaggctccg gcactgatca tcgctcactc caccgtgttt
    14401 gaatcggtac gcaaagaagt caaaggctat gtggttgatc cattaggcaa acatcacttc
    14461 gaaaacgtct ctatcgaata attaaaagcc atacaagact gatggcaaag gcaaaactgc
    14521 ctgatgtgct ccgcttatca ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt
    14581 ttgtaggccg gataaggcgt taacgccgca tccggcataa acaaagcgca ctttgtcaac
    14641 aatctgtata cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc
    14701 attctctgtc cgacaagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg
    14761 ctgcgggtca ctatagagaa tccgggttat gttgcagttt attctccgac gtttgggact
    14821 cgtcatcccc acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc
    14881 gggcgatccg gtgatgatca tggcaggcga acgtgggatc tcccctgagc gtcacgtgca
    14941 gctgctggct gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg
    15001 gggcgtaatg cacggcgatc taggcatttc aatgaaaagc cgaattccag tatgggaaga
    15061 gttcgtgccg cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgctac
    15121 ggcagtcggt attccggttg gtgtgctggc tgcggttaaa cgcggttcca ttttcgatca
    15181 cactgcagtt ggcctggcgc tgaccggcta ctcgatgccg atcttctggt ggggcatgat
    15241 gctgatcatg ctggtttcgg tgcactggaa cctgacgccc gtctccggtc gcgtgagcga
    15301 tatggtgttc ctcgatgact ccaatccgtt aaccggtttt atgctaatcg acaccgccat
    15361 ctggggtgaa gacggcaact ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt
    15421 gctgggtact attccgctgg cggtcattgt gcgtatgaca cgctcctcga tgctggaagt
    15481 gctgggcgag gattacatcc gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat
    15541 tatcgtccat gcgctgcgta acgcgatgct gccggtagtg accgttatcg gcctccaggt
    15601 gggaacattg ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg
    15661 gcgctggttg attgacgcac tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt
    15721 gctggtggcg acgatgatta tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt
    15781 gaacccgcgt attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa
    15841 agtgattagc gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg
    15901 caacaaaggc gcggtcgtcg ggctggttta cgtcgccatc gtgctgttca tcgcgatctt
    15961 tgccaactgg attgcgcctt ataacccggc ggatcagttc cgcgatgcgc tgctcgcccc
    16021 gccagcctgg caggaaggcg gcagtatggc acacttgctg ggcaccgatg acgtcggtcg
    16081 tgatgtgctg tcgcggctga tgtacggcgc gcgtttatcg ctgctggttg gctgtctggt
    16141 ggtggtgttg tcgctgatta tgggcgttat tctcggcctg atcgccggtt acttcggcgg
    16201 cctggttgat aacattatca tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct
    16261 gttggcactg gtgctggtgg cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct
    16321 gaccttcgtt gccttgccgc actatgtgcg cttaacccgc gccgccgtgc tggtggaggt
    16381 gaatcgcgat tacgtcaccg cctctcgcgt ggcaggtgcc ggggcgatgc gccagatgtt
    16441 tattaacatc ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc tcggtttctc
    16501 taacgccatt ctcgatatgg ctgctcttgg tttcctcggc atgggggcac agccgccaac
    16561 gcctgagtgg ggcaccatgc tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt
    16621 cgtgaccttc cctggtctgg cgatcctgtt gacggtgctg gcatttaacc tgatgggtga
    16681 cggtctgcgt gacgcgctcg atcccaaact gaagcagtaa gaggttcgag atggcgttat
    16741 taaatgtaga taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag
    16801 accgcatcag ctacagcgta aaacagggcg aagtggtcgg gattgtgggt gagtccggct
    16861 ccggtaagtc ggtcagttca ctggcgatta tggggctgat tgattatccg ggccgcgtaa
    16921 tggcagaaaa actggagttt aacggccagg atttgcagcg tatctcagaa aaagagcgcc
    16981 gcaacctggt gggtgccgaa gtggcgatga tcttccagga cccgatgacc agccttaacc
    17041 catgctacac cgtgggtttc cagattatgg aagcgattaa agtgcatcag ggcggcaaca
    17101 aaagtacccg ccgtcagcgg gcgattgacc tgctgaatca ggtcggtatt cccgatccgg
    17161 cttcgcgtct ggatgtttac ccgcatcagc tttccggcgg catgagccag cgcgtgatga
    17221 tcgccatggc gattgcctgt cggccaaaac tgctgattgc cgatgaaccg accaccgcgc
    17281 tggacgtgac cattcaggcg caaatcatcg aactactgct ggagctacag cagaaagaga
    17341 acatggcgct ggtgttaatt acccatgacc tggcgctggt ggcggaagct gcgcataaaa
    17401 tcattgtgat gtatgcaggt caggtagtag aaacgggcga tgcacacgcc atcttccatg
    17461 cgccgcgtca cccgtatact caggcattgc tgcgtgcgct gccagaattt gctcaggaca
    17521 aagaacgtct ggcgtcgttg ccaggtgttg ttcccggcaa gtatgaccgc ccgaacggct
    17581 gcctgcttaa cccgcgctgc ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc
    17641 tgaatatgct cgctgatggg cgtcagtcca aatgccatta cccacttgat gatgccggga
    17701 ggcccacact atgagtacgc aagaggccac ctcgcaacaa ccgctgttgc aggctatcga
    17761 cctgaaaaaa cattatccgg tgaagaaagg catgttcgtg ccggaacgtc tggtgaaggc
    17821 gctggacggc gtttcgttta accttgaacg tggcaaaacg ctggcagtag tgggcgaatc
    17881 tggctgcggt aaatcgaccc tcggtcggtt gctgacgatg atcgaaacgc ccaccggcgg
    17941 cgagctgtat tatcaggggc aggatctgct caagcacgat ccgcaggcgc agaagctgcg
    18001 tcggcagaaa atccagatcg tcttccagaa tccctacggt tcgctgaatc cgcgtaagaa
    18061 agttgggcaa attctcgaag agccgctgct tattaacacc agcttaagca aagaacagcg
    18121 tcgggaaaaa gccctgtcga tgatggcaaa agtcggcctg aaaaccgagc actatgaccg
    18181 ctatccgcac atgttttccg gcggacaacg tcagcgtatc gctattgccc gtggtctgat
    18241 gctcgacccg gatgtggtga ttgccgatga gccggtttct gcgctggacg tctcagtgcg
    18301 tgcgcaggtg ctgaatctga tgatggattt gcagcaggag ttggggcttt cttatgtctt
    18361 tatctcccac gacctgtcgg ttgtggagca cattgctgat gaagtgatgg tgatgtacct
    18421 aggccgctgc gtggagaaag gaacgaagga ccaaatcttc aataacccgc gccatccgta
    18481 cacccaggcg ctgctctccg cgacgccacg cctgaacccg gacgatcgtc gtgaacgcat
    18541 caaactcacc ggtgaactgc caagcccgct gaatcctccg ccgggttgcg ccttcaacgc
    18601 ccgctgtcgt cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa aagactacgg
    18661 aggtcaactg gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc gttaactcac
    18721 aaaaccgggg ccatgcgctc cggtttactc aatcagcttc aacagtggcg acaagcatag
    18781 caaaatgccg taaagcagaa tcagccaggc tttaaatccg cgaagttttt ccagttgtgc
    18841 gactttatag atcaggaaaa acggaatgag gcacgacaca atgccatata acgggcttcc
    18901 caactgaaag aacaccagca ctgatacacg aaacgaaacc caaatcgtca acgtaatgac
    18961 gataaaagcg cagatcgcca gagtcagcat gcgtgagtta attttcttgg tatcaataat
    19021 tcggcttaac agattgagaa taatgccttt aatagcctcg tggaaaccga gataaatgcc
    19081 aaagaatgcg gtcagtacgg caaagatatt cagcaccgta gaggtgatat gaatgatatg
    19141 tccagggatc acctgcgcgg ccagtgccag tgccgagata ttttgttcaa aggcagaaac
    19201 ggcttcttcg tggctaattg agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat
    19261 gagcgtgata taactaattc ggtgggtacg cagcgcgagc cgggttgcca gtactttatc
    19321 tgcttcccgt ttacgatagg caatattcat tgggttaagt acctgaataa ataccgcaga
    19381 aaagaagcaa aatggaatgg taagcaggac atcgcggaaa aagacggagg cttgcgggaa
    19441 ggcggttata ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca cgacaataat
    19501 ccctactttg accaccacca ttggcccaga aatcttaaac agtaatcgtt caccaccaga
    19561 cgcaatcgct atcagtacgg cgaaaatagc gactttataa agtagagatt gtgaaagatc
    19621 ggcatcggtt aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca cggagagaga
    19681 gtagataaaa atcccgtgga taatcatcaa aaagtagata accccgagga aaattcccca
    19741 gttcttcccc aggtaatgac tgataatatc ggtgtagtca ttacaggaat cgctttcaga
    19801 aagggttttt aaataaatgt cctgcactac ccaggtggca ggataagcaa tgatcgctgc
    19861 ggtaataaat acccaaattc ccttcaagcc aatttgtact ggcatcagca cggttccggc
    19921 accaatcgcc atgcctatgc ataataaaac ccagccgaag tcgtagcgtg taaacggcaa
    19981 tttctgattg tgtttcgata gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg
    20041 gcctcgcaaa aatgaataaa cccagtttct cttgcgcaac aggtggagaa gtcgaatacg
    20101 cagtatgtaa aaaatgaggc aacagattca ctggggaggt cgcgaggagg cgcaattcta
    20161 caaaattgcg catttcacta ccagtctgga gtcagcccgg atatgaactg caactattgc
    20221 aaataaagaa attaagctgg aaggcatcgc gcaatgagga agattgccgg gtgctgacag
    20281 taaaaagcaa tcgtaaaatc agcggcaagc cggaagggtt ccggtgaggc gcaatgttgc
    20341 gggggcttta tccctggtgg cattggttgc tggagagaga aaacccccgc acgttgcagg
    20401 tatgtacctg acaacataac gggggctaat cttgaaccta gaccactcaa gaatagccgc
    20461 gaaacgttgt cattacaaca caggcggcta tatgacgctc gcagagctgg gcatggcctt
    20521 ctggcatgat ttagcggctc cggtcattgc tggcattctt gccagtatga tcgtgaactg
    20581 gctgaacaag cggaagtagc gtgtcatg
//
LOCUS       DAJTXY010000069        20548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTXY010000069 DAJTXY010000000
VERSION     DAJTXY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.069"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO566_002541"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO566_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6605780.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO566_002542"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO566_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6605781.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO566_002543"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO566_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6605782.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO566_002544"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO566_002544"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6605783.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFCKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO566_002545"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO566_002545"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6605784.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4448..4813
                     /locus_tag="OO566_002546"
     CDS             4448..4813
                     /locus_tag="OO566_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6605785.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4963..5481
                     /locus_tag="OO566_002547"
     CDS             4963..5481
                     /locus_tag="OO566_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6605786.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5471..6696
                     /gene="dgcN"
                     /locus_tag="OO566_002548"
                     /pseudo
     CDS             5471..6696
                     /gene="dgcN"
                     /locus_tag="OO566_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6712..7194
                     /locus_tag="OO566_002549"
     CDS             6712..7194
                     /locus_tag="OO566_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6605787.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQLQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="OO566_002550"
     CDS             complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="OO566_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6605788.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="OO566_002551"
     CDS             complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="OO566_002551"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6605789.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="OO566_002552"
     CDS             complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="OO566_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6605790.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="OO566_002553"
     CDS             complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="OO566_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6605791.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="OO566_002554"
     CDS             complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="OO566_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6605792.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10938..11729
                     /locus_tag="OO566_002555"
     CDS             10938..11729
                     /locus_tag="OO566_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6605793.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11750..13036
                     /locus_tag="OO566_002556"
     CDS             11750..13036
                     /locus_tag="OO566_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6605794.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="OO566_002557"
     CDS             complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="OO566_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6605795.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13807..14685
                     /gene="nadK"
                     /locus_tag="OO566_002558"
     CDS             13807..14685
                     /gene="nadK"
                     /locus_tag="OO566_002558"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6605796.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14771..16432
                     /gene="recN"
                     /locus_tag="OO566_002559"
     CDS             14771..16432
                     /gene="recN"
                     /locus_tag="OO566_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6605797.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16581..16922
                     /gene="bamE"
                     /locus_tag="OO566_002560"
     CDS             16581..16922
                     /gene="bamE"
                     /locus_tag="OO566_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6605798.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16984..17274)
                     /locus_tag="OO566_002561"
     CDS             complement(16984..17274)
                     /locus_tag="OO566_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6605799.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17264..17701)
                     /gene="ratA"
                     /locus_tag="OO566_002562"
     CDS             complement(17264..17701)
                     /gene="ratA"
                     /locus_tag="OO566_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6605800.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17872..18354
                     /gene="smpB"
                     /locus_tag="OO566_002563"
     CDS             17872..18354
                     /gene="smpB"
                     /locus_tag="OO566_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6605801.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18569..18931
                     /gene="ssrA"
                     /locus_tag="OO566_002564"
     tmRNA           18569..18931
                     /gene="ssrA"
                     /locus_tag="OO566_002564"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19136..20377
                     /locus_tag="OO566_002565"
     CDS             19136..20377
                     /locus_tag="OO566_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6605802.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDDDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5305 a         5368 c         4822 g         5053 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac catcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcag aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccaa gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat atataaattt acaccccttt gaaaacaatc cgctatgctt tgaaaaagga
     4441 gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct ttactggtta gcggatgtca
     4501 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4561 ggaagatgcg atatcggaca gcgccataaa agatgacgat gcatttagcg attcacaggc
     4621 ggatcgcggt ctatacctta aaggatatgc cgaagggcaa aagaaaactt gccagaccga
     4681 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgca acaatgttga
     4741 aaatgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
     4801 acgattaaat taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt
     4861 ttatttctca cattgatgac ggtcgcatga aaaaatgata ttttaatgag catcctgaaa
     4921 aaatatcgat gcatttcgag cgaagatggt ggggatccct gaatgcgttt ttctcaccga
     4981 ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca agagccttct
     5041 gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct
     5101 gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta
     5161 caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag
     5221 gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg
     5281 gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca aaataccgag
     5341 tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta
     5401 aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca
     5461 cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc
     5521 attacgcaac atcagtatga ccagcatatt tatcactatg atgttgatct ggttgctgct
     5581 ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc
     5641 agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac
     5701 tgaaacactg gcagcgctgg gccagcaagg gcattttcaa ctgcagaagt acgtgataag
     5761 cagcaaaata ttctggcatc ctggcattac ccccgtaagg atccaggcga tactttcagc
     5821 aatttcataa gccactggct cttccctgcc cccatcattc agccgattcg tcacaatggt
     5881 gaaaccattg gcgaagtacg cttaaccgct cgcgacagtt caattagcca ttttatctgg
     5941 ttttcactcg ccgtactgac cggttgtatt ctgctggcat caggcatcgc aattaccctc
     6001 acccgccatt tgcacaatgg cctggtggaa gcactgaaaa atatcaccga tgtcgtacat
     6061 gatgtgcgtt ccaaccgcaa tttttcccga cgagtttcgg aagaacgtat cgctgagttt
     6121 caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg gcagcttcgt
     6181 ttacaggcca aaaatgcgca gcttctgcgt accgcgctac atgacccatt aaccgggctg
     6241 gctaaccgcg cagcgtttcg tagcggcatc aatacgttga tgaacaattc cgatgcccga
     6301 aaaacgtcgg cgttactatt tcttgatggc gataatttca aatatatcaa tgatacctgg
     6361 ggtcatgcga cgggcgatag ggtcttgatt gaaatcgcaa aacggttagc tgaatttggc
     6421 gggctgcgac ataaagcata ccgcctgggc ggcgatgaat tcgctatggt gctctatgat
     6481 gtacagtcgg aatctgaagt gcagcagata tgctcagcac tgacacaaat ctttaatctc
     6541 ccgtttgatc ttcataatgg ccatcagacc accatgacat taagcattgg ttacgcgatg
     6601 accattgagc acgcctctgc ggaaaaatta caagagcttg ccgatcacaa tatgtatcag
     6661 gccaaacacc agcgtgccga aaagctggtg agataacaag gatatatcga tatgataaag
     6721 cacctggtag cacccctgat tttcacctca ctaatactga ctggctgcca gtcccctcag
     6781 ggaaagttta ctcctgagca agtcgccgct atgcaatctt atggatttac tgaatccgcc
     6841 ggcgactggt cgctgggctt atcagatgcc attctgttcg caaaaaatga ctacaaattg
     6901 ctcccggaaa gccagcaaca gctccaaacc atggcagcca aattggcctc gacagggcta
     6961 acacatgccc gtatggatgg acacaccgat aactatggtg aagacagtta caacgaaggc
     7021 ttatcattga aacgggcgaa tgtcgtggcc gatgcatggg ctattggtgg acaaattcca
     7081 cgcagcaatc tcaccacaca gggtttagga aaaaaatatc ccatagccag taacaagacc
     7141 gcccagggcc gcgccgagaa ccgccgcgtc gcagtggtga ttacaacccc ttaaaacacg
     7201 agcaaacaga caaaaaaagg gccagccaat tgcccagccc ttcttaacag gatgtcgctt
     7261 aagcgaaatc ttagttaaga cgctctttga tacgagcagc cttaccagta cgctcacgca
     7321 ggtagtacag tttagcttta cgaacagcac cacgacgttt gacagaaatg ctgtcaacta
     7381 ccggagagtg agtctggaag acacgctcaa cgccttcgcc gttggaaatt ttacgaacag
     7441 tgaatgcaga gtgcagaccg cggttacgaa tagcgataac cacgccctcg aatgcctgca
     7501 gacgtttttt ggaaccttca acaacccata ctttcacttc cacggtatca cccggacgga
     7561 aggaaggtac gtcctgcttc atctgctctt gttcaagttg cttaataatg ttgctcataa
     7621 tttaatctct tatcctgggt aaactgatct ctcgggggct tacgccatcc catcatgttt
     7681 atgttgctgt tgtgcgtgtt ccgttttgaa ctccgccagc aaccttgctt gctcttcagt
     7741 cagagccagg ttttccagaa gttcaggtct tctaagccag gtacggccca gcgactgttt
     7801 caaacgccag cgacgtatct cggcatggtt gcccgacagt aacactggcg gaacttccat
     7861 cccttctaac acctcaggcc gcgtatagtg cgggcaatcc agcaatcctt cagcaaagga
     7921 atcttccgtt gccgaggctt catgtcccag tactcccgga ataaaccggg aaacggagtc
     7981 aatcagcgtc attgctggta actcaccacc actgagaacg taatcgccga ttgaccattc
     8041 ttcgtcaatt tcggtttgga tcacgcgctc atctatacct tcgtagcgac cgcacaccag
     8101 aatcaatttt tgattcgttg ccagttcgct gacgcccgct tgatcaagct tgcgtccctg
     8161 tggtgacaga taaatcacct ttgcgccttc acccgccgcg gcttttgctg catgaatggc
     8221 gtcccgcaag ggttgcacca tcattaacat ccccggtccg ccgccgtaag gacgatcgtc
     8281 cacggtacgg tgccggtcat gcgtgaagtc gcgaggactc caactctgga tgctcagcag
     8341 gccattttta actgcccggc cagttacccc gtaatcggta attgcgcgga acatttcagg
     8401 aaacaggcta attatgccaa tccacatagc gccgtctttt accgtttatc cggtggttta
     8461 aaaaccagga tcccaatcta cttcgattga acgagtagtg agatcgactt tcttgataac
     8521 ctgcccatcg aggaacggta cgagacgttc cttgatacca aacgcatctt tcaggtttgc
     8581 cttaatgacg agaacgtcat tagatccggt ttccatcata tcgacgactt taccgagatc
     8641 gtagccttca gtggttacta cctggcagcc catcaggtct ttccagtagt agtccccctc
     8701 ttcaagctga ggcagctgcg atgaatccac gacaatttca caattcgtca gcaggttcgc
     8761 cgcatcacga tcgtcaacgc ctttcagctt gatgatcatg tcctgattgt ggtgcttcca
     8821 gctttccagc tggacttgct gccactgacc cgccttctgg ataaaccagg gctgatagtc
     8881 aaaaatgctt tcggcgtctt cggtggaaga aaacactctg agccacccac gaataccgta
     8941 agacgaaccc atttttccca aaacgatggg atcaacaggt gcttgcgcgg tgagttgttt
     9001 gctcatcatg accaccgtga cagattaagc tgctttgttt acttctttga tcagcgcagc
     9061 aacgcgatca gaaatagttg cgccctggcc aacccagtga gcgatgcgat ccagatccag
     9121 gcgagtgcct tcttcttttt cgctagcgat tgggttgaag aaaccaacgc gctcgatgaa
     9181 gcgaccgttg cgtgcattac ggctgtcagc gacaacaacc tggtagaacg gacgcttttt
     9241 agcgccgtga cgtgctaaac gaatagttac cataacatcc tcttgtgtga ataaaacaac
     9301 cggaccccat cgaggaacgg agtccggtgt catattaaaa gcccgaaaat tttactgatt
     9361 tctggagaaa atgcaatcag cagttaataa ctctgtcgca taaggtcatt tagcgaccag
     9421 gaaatcccgg cggcatcatt cccttcatgc ttctcatcat cttcgccatt ccgcctttct
     9481 tcattttctt catcatgcgc tgcatgtcgt cgaactgctt cagaaggcgg ttaacgtcct
     9541 gtacctgcat cccgcaaccg gcggcgatac gacgcttacg cgaacctttg atgatttctg
     9601 gcttagcgcg ctctttcatc gtcattgagt tgatgatggc ttccatacgc accagcactt
     9661 tatcgtccat ctgcgacttg acgttatccg ggatctgccc catgcccggc agtttgccca
     9721 tcaagctggc catgccgccc atatttttca tctggcgcag ttgctcaaga aagtcgttga
     9781 gatcgaagcc gtcgcctttt tttagcttgc tggctaattt ctctgcctgc gcgcggtcaa
     9841 ctttgctttc gatatcttcg atcagcgaca gtacgtcgcc catgccgaga atacgcgacg
     9901 cgatgcggtc cggatggaac ggctccagcg cctcagtttt ctcgccaaca ccgaggaact
     9961 tgatcggttt gccagtgatg tgacgaatag agagcgccgc accgccgcgg gcatcgccgt
    10021 ccactttggt caacactacg ccggtaagcg gtaacgcttc attgaatgct tttgccgtat
    10081 tggccgcatc ctgaccggtc atggcgtcaa ccacaaacag ggtttcaacc gggttaatcg
    10141 acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc aacgtgcaga cgaccagcgg
    10201 tatccaccag cagcacgtcg tagaatttca gtttggcttc tttcagcgcc gcgttaacaa
    10261 tatcaaccgg cttctgacca acatcagaag ggaagaaatc aacgcccacc tgctctgcca
    10321 gcgtctcaag ctgtttgatt gccgccgggc gatagacgtc agcagaaacc accagcactt
    10381 tcttcttgtg cttctcgcgc aggaaattac cgagtttacc aacgctggtt gttttaccgg
    10441 caccttgcag gcctgccatc agcacgaccg caggcggttg cgcagccaga ttcagggtct
    10501 ggttctcttc gcccatcgcc gcaaccagtt cgttacggac gattttgacg aactcctgcc
    10561 ccggcgtcag gctcttatta acttcatgac caaccgcttt ctcttttacg cgattgataa
    10621 actcacgcac taccggcaga gcgacgtccg cctccagcag cgccatgcgc acttcgcgca
    10681 gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc actgatattg cgcagcgtgc
    10741 gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg cctggggtgg aaacggttgg
    10801 ccgcaatcgc gacacatcat cagtattttg ccgcagtata acatgaaggc gtctttgttg
    10861 ttatgcaacg gttggagcag cgttcacctg acgctatact gcttctcttt cttattgctc
    10921 aaactgtcga cattactatg cccgtttttg ctctgctcgc gcttgtcgcc tactccgtca
    10981 gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg cggctggcgg cgcatggcta
    11041 ttatttctgc ggtcattgcg ctggtctgcc acgcaatcgc tctggaagcc cgcatcctgc
    11101 ccgacggtga tagcggacaa aacctcagcc tgctgaacgt tggttcattg gtcagtttgg
    11161 tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg tggctggttg ctgctaccca
    11221 ttgtctatgc ctttgcgctt atcaacctgg cgctggcgac cttcatgccc aatgaataca
    11281 tcacccatct ggaagcaacg cctgggatgc tggtgcacat tggcttatcg ctcttttcct
    11341 atgccacgct aattatcgcc gccctgtacg cgctgcaact ggcgtggatt gattaccaac
    11401 tgaagaacaa gaagctggca tttaaccagg aaatgccgcc attgatgagt atcgagcgta
    11461 aaatgttcca catcacgcag attggcgtgg tgctgctaac gctcacgctt tgcactggcc
    11521 tgttctacat gcacaaccta tttagcatgg aaaatatcga caaggctgtg ctctctatcg
    11581 tggcgtggtt tgtctatatt gtgctgctgt ggggacatta ccatgaagga tggcgtggac
    11641 gccgcgtcgt ctggtttaac gttgcgggcg cggtaattct gacactggcc tacttcggca
    11701 gccgaattgt ccagcagtta atcagctaaa cccagaaaag gagtttcccc tggaacacat
    11761 ttctactact acgttgatca ttattctgat catcatggtg gtcatttcag cctatttttc
    11821 cgggtccgaa accggaatga tgaccctcaa ccgctatcgt cttcgacata tggcgaaaca
    11881 gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt aagccagacc gcctgataag
    11941 cctggtgtta atcggcaata acctggtcaa tattcttgcc tccgcgctcg gcactattgt
    12001 cgggatgcgt ttgtacggcg atgcgggcgt ggcaattgcg actggtgtgc tgacttttgt
    12061 cgtactggtt tttgctgagg tattgccgaa aaccattgcc gcgctgtacc cggaaaaagt
    12121 cgcttatccg agtagttttc tgctggctcc gctgcaaatt ttgatgatgc cgctggtctg
    12181 gttgctgaat gctatcaccc gtatgctgat gcgcatgatg ggtatcaaaa ccgatatcgt
    12241 ggttagcggc tctttgagca aagaagagtt gcgcactatc gtgcatgaat cgcgctcaca
    12301 aatttcccgt cgcaatcagg atatgctgct gtcggtgctc gatctggaaa aaatgaccgt
    12361 tgatgacatc atggtgccgc gcagtgaaat tatcggtatt gatatcaacg atgactggaa
    12421 atctattctg cgccaactct cccactcacc tcacgggcgc atcgtgcttt accgtgattc
    12481 gctggacgac gccatcagta tgctgcgagt acgtgaagcc tggcggttga tgtcggagaa
    12541 aaaagagttc accaaagaaa ccatgctgcg cgccgcggac gagatctatt ttgtgcctga
    12601 aggtacgccg ctcagcacgc agttggtaaa gtttcagcgc aacaaaaaga aagtcggcct
    12661 ggtcgtcaac gagtatggag acattcaggg gctggtgacg gttgaagata ttctggaaga
    12721 gattgtcggc gatttcacta cgtcgatgtc gccaacactt gccgaagagg tcacgccgca
    12781 aaacgacggt tcggtgatta tcgatggcac cgccaacgtg cgggaaatca acaaagcctt
    12841 taactggcat ctaccggaag atgatgcccg cacggttaat ggcgtcattc ttgaggcact
    12901 ggaagagatc cctgtcgcag gcacccgcgt gcgtattggc gagtacgata tcgatattct
    12961 cgacgtacag gacaatatga ttaagcaggt aaaagttttt cctgtgaaac cgctgcgcga
    13021 gagtgtggcg gagtaacgaa aacggccctg cattcgcatg cagggccgtg aattattacg
    13081 aaaacagaaa ttacgctttt gctttcgcta cagttaccat cgccgcacga atcgtacgac
    13141 cattcagcgt ataacccttc tgcataatgc ccagtacgtt acctggcgca acgtcatcag
    13201 attccaccat tgcgatagcc tgatgcacat tcgggtccag cggcacgtta gtttcggcga
    13261 tcacttcaac accaaactta cgcacaacat ccagcatcga cttcagcgtc agctcaatgc
    13321 cttcaaccat cgcagacata tccgggttag ctttatcagc cacttccagc gcacgatcca
    13381 ggctatcaat caccggcagc aattcgttga tgaatttctc cagcgcgaat ttgtgggctt
    13441 tttcaatatc cagttcagta cgacgacgca ggttttccat ttcggctttt acgcgcaaaa
    13501 taccgtcacg ttcacgggtc tgggcttcag ccagctgagc ttcgagattc gcaatttttt
    13561 catcgcgcgg atccacctgc tcagcagaag cttctggctc aactgcctca atctcttcgt
    13621 gctgatccat gataatttct tccggggctt gcccctcagg cgttttctgt tctttactac
    13681 tcatgaattt ctccgcgttt ttttcgcatt catctcgcta acttcgctta ttatggggat
    13741 cagtttcagg gtttcaaggg aagcactcac attgtcatca atcttcgcaa caaggacctc
    13801 ggaaaaatga ataatcattt caagtgcatt ggcattgtgg gacacccacg gcaccctact
    13861 gcactgacaa cacatgaaat gctctaccgc tggctgtgca caaaaggtta cgaggtcatc
    13921 gttgagcaac aaatcgctca cgaactgcaa ctgaagaatg tgaaaactgg cacgctcgcg
    13981 gagattgggc aactggctga tctcgcggta gtcgttggtg gcgacggtaa tatgctgggc
    14041 gcggcgcgca cgctcgcccg ttacgatatt aaagttattg gaatcaaccg tggcaacctg
    14101 ggtttcctga ctgaccttga ccccgataac gcccagcaac aattagccga tgtgctggaa
    14161 ggtcactaca tcagcgagaa acgttttttg ctggaagcgc aagtctgtca gcaagattgc
    14221 caaaaacgca tcagcaccgc gattaacgaa gtggtacttc atcctggcaa agtggcgcat
    14281 atgatagagt tcgaagtgta tatcgacgag atctttgcgt tttctcagcg atctgatggg
    14341 ctaattattt cgacgccaac aggctccacc gcctattccc tctctgcggg cggtcctatt
    14401 ctgaccccct ctctggatgc gattaccctg gtgcccatgt tcccgcatac gttgtcagca
    14461 cgaccactgg tcataaacag cagcagcacg atccgtctgc gtttttcgca tcgccgtaac
    14521 gacctggaaa tcagttgcga cagccagata gcactgccga ttcaggaagg tgaagatgtc
    14581 ctgattcgtc gctgtgatta ccatctgaat ctgattcatc cgaaagatta cagttatttc
    14641 aatacattaa gcaccaagct cggctggtca aaaaaattat tctaatttta cgccagcctc
    14701 tttactgtat ataaaaccag tttatactgt acacaataac agtaatggtt tttcatacag
    14761 gaaaacgact atgttggcac aactgaccat cagcaacttt gctatcgttc gtgagcttga
    14821 gattgatttt catagcggca tgaccgtaat aactggcgag accggtgcgg gtaaatctat
    14881 tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct gaagccgaca tggtgcgtac
    14941 cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg aaagatacgc cagcggccct
    15001 gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa tgtttgcttc gtcgcgtgat
    15061 cagcagtgat ggtcgctccc gtggtttcat caacggtaca gctgttcctc tgtcacaact
    15121 gcgcgaactg ggtcagttgc tgattcagat ccatggtcag cacgctcatc aattactcac
    15181 caaacctgag caccaaaaat tcctgcttga tggctatgcc aatgaaacct ctctacttca
    15241 ggaaatgacc gcacgttatc agttgtggca tcaaagctgc cgtgacctcg cgcatcatca
    15301 acagttaagt caggaacgcg ccgcccgtgc ggaactgctg caataccaat taaaagaact
    15361 taacgaattt aatccgcagc ccggagagtt tgagcaaatc gacgaagagt acaaacgtct
    15421 ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca ttggcattaa tggccgacgg
    15481 tgaagacgca aacctgcaaa gtcagcttta cacggctaaa caactggtga gcgaattgat
    15541 tggcatggac agcaaactgt ccggcgtact tgatatgctg gaagaagcta ccatccagat
    15601 tgctgaagcc agcgacgaac tgcgccacta ctgcgatcgt ctggatctcg atcccaaccg
    15661 actgtttgaa cttgaacagc gcatctcaaa acagatttcg ctggcacgta aacatcacgt
    15721 cagccctgag gcattaccac agtattacca gtcgctactg gaagaacagc agcaactgga
    15781 cgatcaggcc gactcacaag aaacgctcgc gctggcggta acgaaacatc atcagcaggc
    15841 actggaaacc gcgcgcgcat tacaccaaca acgccagcaa tatgcagaag aacttgcaca
    15901 gctgatcacc gacagtatgc atgcgctctc aatgccgcat gggcagttta cgattgatgt
    15961 caaatatgac gagcatcacc tgggcgctga cggtgccgat cgtattgagt ttcgggtaac
    16021 caccaaccca ggtcagccaa tgcagcctat tgccaaagtc gcatccggtg gtgaattgtc
    16081 ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa atggaaaccc cggcactgat
    16141 ttttgatgaa gtggatgtag ggattagcgg tccaacagcg gcagttgtcg gcaaactgct
    16201 gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc cacctgccac aagtcgcggg
    16261 atgtggtcat caacactatt ttgtcagcaa agaaaccgat ggtgcgatga cagaaacaca
    16321 tatgcaatct ctggataaaa aagcgcggtt acaagagctg gcgcgcctgc ttggtggcag
    16381 tgaagtcaca cgtaacacac tggcgaatgc gaaagaactg cttgcagcgt aaactttttt
    16441 cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga ccggaaagca aaaggtttta
    16501 aagtgatgaa aggtctatta tcatcggcat attacagatg agccacgtac tgctcgggcc
    16561 cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac tgctgcagca gcagtactat
    16621 tgatgttgac cgcaggctgt tccactctgg agcgagtggt ttaccgtcct gacatcaacc
    16681 aggggaacta tctgaccgct aacgacgtat ccaaaatacg tgttggcatg acgcaacaac
    16741 aagttgcgta cgcattgggt acaccgctga tgtccgatcc atttggtacg aatacctggt
    16801 tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac gcagcaaacg ctgacgctga
    16861 cctttaacag tagcggtgtg ttgaccaata ttgataacaa acctgcgctg agtggtaact
    16921 aataataaag ttgctctcaa agacgttaaa aaaggtgctc aatgagcacc ttttttctgt
    16981 ctgttattta ttcgctgatt tttctgctcg ttgcctgcga agctctttcg gatcggcaat
    17041 gagagggcga taaatctcca cccgatcgcc atcatgcaca ctatcgctta gttttgccgg
    17101 acggctgtaa atgccgactt tgtttttagt taaatcgata tcggtacgca attccagcaa
    17161 gccactggcg cgaatagctt cttcaaccgt cgcgccctcc tgcagcgtca ctcgctgcag
    17221 gtactgcttc tcaggtagcg cataagccac ctcaacggca attttaccta gcactgtaaa
    17281 cctctttcgc acgaaccgta aaagcctgga ccatattagc cgccagctct ttaaacacgc
    17341 gaccaaaggc gagttcaatc aacttattgg taaactcaaa gtcgagatga aactcgatac
    17401 gacacgcctc ctggctcagc ggcgtaaact tccatccacc aatcaatttc ttgaacggcc
    17461 catccaccag attcataaga atactttggt tactggtcaa ctggttgcgg gtagtaaacg
    17521 ttttgctgat cccagcctta gagacatcta ccgccgcagt catctgccca ggagtggact
    17581 ccagaatccg acttccggta caacctggca aaaactgagg ataagactga acgtcattca
    17641 ctaactgata catttgctcc gcgctgtagg gtaccagtgc ggtccggcta atctgaggca
    17701 taacaatttc catcaacaaa aatccaacaa ataatatcat ttatcctgct gtaaaaaaaa
    17761 cgctatcccg gcgctgggta acatcgggtt catgctaaga tagagccttg tcccccgcag
    17821 gattgatatg gggtgttttc gatttcagat taccgatgat tcacgacgct tatgacgaag
    17881 aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta acaagcgcgc ccgtcacgaa
    17941 tactttatcg aagaagagtt cgaagcggga cttgccctgc aaggctggga agttaaatcc
    18001 ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc ttctgcgtga cggagaggca
    18061 tttctgtttg gcgctaacat cacgccaatg gccgtggcct ccacgcatgt ggtgtgcgat
    18121 cctacccgta cccgcaagtt acttctcaac cagcgcgaac tggactcatt gtacggtcgc
    18181 gtcaatcgag aaggctatac cgtagtggcg ctctccctgt actggaaaaa tgcctggtgc
    18241 aaagtgaaaa tcggcgtcgc caaaggtaaa aaacagcacg ataaacgttc agatatcaaa
    18301 gaacgcgaat ggcaggtgga taaagcacgc atcatgaaaa acgcccaccg ttaaacctgc
    18361 actccaatta ttgaccagtt cctcaccgcg cctccctctc cggcggcgcg aatgaacatc
    18421 ttattggcta tcacatccga cacaaatgtt gccatcccat tgcttaatcg aataaaaatc
    18481 aggctacatg ggtgctaaat ctttaacgat aacgccattg aggctggtca tggcgctcat
    18541 aaatctggta tacttacctt tacacattgg ggctgattct ggattcgacg ggatttgcga
    18601 aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa aatagtcgca
    18661 aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct ctccctagcc
    18721 tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg tggaagccct
    18781 gcctggggtt gaagcgttaa aacttaatca ggctagtttg ttagtggcgt gtccgtccgc
    18841 agctggcaag cgaatgtaaa gactgactaa gcatgtagta ccgaggatgt aggaatttcg
    18901 gacgcgggtt caactcccgc cagctccacc aaaattctcc atcggtgatt accagagtca
    18961 tccgatgaag tcctaagagc ccgcacggcg caagccctgc gggctttttt gtgccccgaa
    19021 tttgtcccgc gaagtctgat gccaactaat taaatccgat ccttttaggc accttgttag
    19081 gcacttcata aagctatatt gtctttgagg tgcctaaaac tatggaaacc cggcaatggc
    19141 aagacaaacc aaacctctat ccgttaagga aatcgaatct gctaaaccca aggaagcgga
    19201 ctacgttctc tatgatgacg atggccttga gctactcatt aaatccagcg ggagtaaaat
    19261 ctggcagttt cgctacattc gccctgtcac caagacacga gcaaagaaaa gcataggccc
    19321 ttacccgtca gttacccttg ccgatgccag aaactaccgt gcagagtccc gctcactcct
    19381 agctaaacag attgagccgc aggaacatca gcaagaacaa ttgcgtagtt cgctggaagc
    19441 caaaaccaat actttccagc tcgtagctga acgttggtgg aatgtgaaga aagccagtgt
    19501 gacagaggac tatgcggaag atatctggcg ctctcttgaa agagatgtct ttcctgcgat
    19561 tggcgacgtt agcgttacag atattaaagc tcatacactg gttcaggccg tacaaccggt
    19621 tcaggccaga ggagcactgg aaacggttcg tcgccagtgt caacgtatta atgaggtcat
    19681 gatctatgcc cagaacacag ggctgattga tgctgttccc agcgttaata tcggtaaagc
    19741 cttcgaaaag cctcagaaaa agaacatgcc cagcattcga ccggatcagc tatctcaact
    19801 gatgcagaca atgcgaacag ccagcattag cctttccaca cgctgcctgt tcatgtggca
    19861 acttcttact attacccgcc ctgccgaagc ggctgaagct cgctgggaag aagtagacat
    19921 agaagcgcaa gagtggaaga ttcccgcagc acgcatgaaa atgaaccgcg accatactgt
    19981 tccattgtca gatgaagcaa tagctgtgct ggagatgatg aaaccgctaa gcggcaaccg
    20041 agaatttatc tttcctagcc gtatcaagcc caaccagcct atgaacagtc agactgttaa
    20101 cgcctcacta aaacgcgcag gttttggtgg tgtgcttgtt tcgcatggtc tgagatcaat
    20161 tgccagtacg gctcttaatg aacaaggctt tccgcctgat gccattgagg cagcattggc
    20221 ccatgtggat aagaatgagg ttcgtcgtgc ttataaccgc agcgattacc tggaacagcg
    20281 tcgcccgatg atgcaatggt gggcaaattt tgtgatggca gcggatcgtg gaagcatgat
    20341 tgaaggtggg ataaaaggaa tgaagctagt tgggtgacta aatagctgcg cggcatagtg
    20401 tcccactttc agccagccct tgcaggttaa gccaccagac agtaaacggc aggtgtcgcg
    20461 ttattaggcg cagctattta gtagtaatat taggattggt gattctgcac tccagaagtt
    20521 cggtaatgct gccaacttac tgatttag
//
LOCUS       DAJTXY010000070        20468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTXY010000070 DAJTXY010000000
VERSION     DAJTXY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.070"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..4313)
                     /gene="mukB"
                     /locus_tag="OO566_002566"
     CDS             complement(<1..4313)
                     /gene="mukB"
                     /locus_tag="OO566_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6605803.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     N"
     gene            complement(4313..5017)
                     /gene="mukE"
                     /locus_tag="OO566_002567"
     CDS             complement(4313..5017)
                     /gene="mukE"
                     /locus_tag="OO566_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6605804.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(4998..6320)
                     /gene="mukF"
                     /locus_tag="OO566_002568"
     CDS             complement(4998..6320)
                     /gene="mukF"
                     /locus_tag="OO566_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6605805.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6317..7102)
                     /gene="cmoM"
                     /locus_tag="OO566_002569"
     CDS             complement(6317..7102)
                     /gene="cmoM"
                     /locus_tag="OO566_002569"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6605806.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7238..8017
                     /gene="elyC"
                     /locus_tag="OO566_002570"
     CDS             7238..8017
                     /gene="elyC"
                     /locus_tag="OO566_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6605807.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7994..8887)
                     /locus_tag="OO566_002571"
     CDS             complement(7994..8887)
                     /locus_tag="OO566_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6605808.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9041..9787)
                     /gene="kdsB"
                     /locus_tag="OO566_002572"
     CDS             complement(9041..9787)
                     /gene="kdsB"
                     /locus_tag="OO566_002572"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6605809.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9784..9966)
                     /gene="ycaR"
                     /locus_tag="OO566_002573"
     CDS             complement(9784..9966)
                     /gene="ycaR"
                     /locus_tag="OO566_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6605810.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10018..11250)
                     /locus_tag="OO566_002574"
     CDS             complement(10018..11250)
                     /locus_tag="OO566_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6605811.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11287..12273)
                     /gene="lpxK"
                     /locus_tag="OO566_002575"
     CDS             complement(11287..12273)
                     /gene="lpxK"
                     /locus_tag="OO566_002575"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6605812.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12270..14018)
                     /gene="msbA"
                     /locus_tag="OO566_002576"
     CDS             complement(12270..14018)
                     /gene="msbA"
                     /locus_tag="OO566_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6605813.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14055..16274)
                     /locus_tag="OO566_002577"
                     /pseudo
     CDS             complement(14055..16274)
                     /locus_tag="OO566_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16481..16765)
                     /gene="ihfB"
                     /locus_tag="OO566_002578"
     CDS             complement(16481..16765)
                     /gene="ihfB"
                     /locus_tag="OO566_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6605814.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16925..18598)
                     /gene="rpsA"
                     /locus_tag="OO566_002579"
     CDS             complement(16925..18598)
                     /gene="rpsA"
                     /locus_tag="OO566_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6605815.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18709..19392)
                     /gene="cmk"
                     /locus_tag="OO566_002580"
     CDS             complement(18709..19392)
                     /gene="cmk"
                     /locus_tag="OO566_002580"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6605816.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19565..20329)
                     /gene="ycaL"
                     /locus_tag="OO566_002581"
     CDS             complement(19565..20329)
                     /gene="ycaL"
                     /locus_tag="OO566_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6605817.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4923 a         5670 c         4964 g         4911 t
ORIGIN      
        1 atattttccg gcgctgcgat gatgagctgc atttgcaaac gttcacacaa ttcaaacagc
       61 gtggcgatag aacgagcatc cagtcgcgct gcttcatcga ggaacagcag gcggcaagga
      121 gagatatctt taccgcgcag gcggcgagat tcatcttccc agctttgtac caccatcacc
      181 agaatcgaca taccggtacc aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc
      241 aaccaaccat cggaaccacg gttaacctca acttccattt ccagatagtt gcggtaatcc
      301 agcagttctt cgccaatggt ctgcggcgtg cgctgcccca tatcaatctg cggattaaga
      361 cgctgataca atttcgccaa cgcttccgag aaggtcagac ggttgctgtt aaacagatcc
      421 tgatgctgct cgtgctgttc agagagcaca tccagcagca tggcgtgcgt ttcacgcacg
      481 ttcacgttga gacgcacgct gttcacctga ccaaacgata cgttctgcaa cccctggttg
      541 agcatacgga tacggttctg ctcgcgctga atggttttgc gaatgatgtt cgccacgctg
      601 cgggaactga tcgccagttt ctgttcacgg gaggttaatt cttcggtcag acggctaagt
      661 tcaatctcca tctgttcgat agcttccacc ggatcatcgg tacgaataat atcctgacga
      721 atacgttcac gcagatgctg ataaaccgcc acgaagaact gaattttacg ctccggacgt
      781 ttcggatctt ccgacatgcg cagcacgtcg cgcagatgtt cgttatccgc caccgccaga
      841 cgcagcgcac ctaacgcctt atccgacatg gaacgcaaat catcagcgga gagataagcc
      901 agctcacgac ggtgtaagcg gcgctcaacg ccgttatctt tcaccatgcg catcaccgca
      961 caccagcccg ctttggcggt cactacctgc tcgcgcatct caaagtaatc ccgctccagc
     1021 ttgcgcagtt tgcgggtcag gttgtccatc tccgcttcgc agaaggtaag cgctttttcc
     1081 agttgattgc ggcgtgaacg gttattgctc agttgcgcat gcagctcgtc acggcgaata
     1141 cgcgcccgct cttctgcccc gctatcagca cgcacgccga tatcctgcaa ttcacgttgc
     1201 agatcgttga gtagctcttt tttggtgtcg taagaacttt tcagcgaagc cagcacctgg
     1261 ttgtactgac tcaactgcgc tgcgtgaccg cgcaacgctt cgcgagcgcg ggtacgctcc
     1321 gcttccgact gttccagacg ttcacgcagt ttttcgttga gatcgctgtt accgctaagc
     1381 atttctgccg agtcagaata gctaaagtgc gcacgacgct gcaccacttc cgtcagggca
     1441 aacgcttgct gacgggcatc gcgctgcatc tgctgagagt acgcgtaatc ttcttttaac
     1501 tgttcgaact gttccgggtc gctctgcaat accgaaacga tcggttccag tttcgccagt
     1561 tgattgccaa actgctgaac aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg
     1621 atttcatcga cgcgatccgc caggctgtca tcagccaaca ggttgagacg cggcagaatg
     1681 cggttcagcg ccgtaacgcc ttctttcgcc tgctcaaact gaatacgctg ctgctggtta
     1741 tcattttcat gattactcaa cgcccgctcc agttcgactc gacggctgtt cagttgacgg
     1801 atttctgctt ccgggtcaga ctcaaacgcc accgccagat gactgccgat aaagcggctg
     1861 aacgcctgat gcagacgctg ggttttctgt acatcaaagg agagcgtggc gaagcgttcg
     1921 gaaagcactt cacgctcggc atggaggctt tcaatacggc tttcacgcgc agcacgacca
     1981 aacagcggca cttccgggaa acgtgaataa cgccactgtc gatcggcgat tttcaccacc
     2041 accgcttttt ccagctcatc aacgctgaac acgctatcat cgaatgactg cggatcccct
     2101 tcgatcaaat agagatcttc cgggcaatcg gtcaagcctt ccaggtgttc ggttacctgt
     2161 gacagatctg gcaccacgat ggcgtggcgt gacgggccat acagagccga gaagtacggc
     2221 gcatcttcca ggctaacgtc gtcataaatt tctgacagca gcacaccacc aaaacgctcc
     2281 gccagcgcgt tcagacgctg atcttcagag ccgccaggct ggcttaaacg ttcgatctct
     2341 tcatcgacgg cgtttttgcg cgcgcctact tcatcgcgtt caacaatcgc ctcgcgctca
     2401 cgctccagca actgttgcag atattcagtg acgtcctggc tggaggagaa ctcttcgccg
     2461 cactgttcac tcaactggtt gagactgttt tgcgctgcca gccaaaccgg tgcacgcagc
     2521 atcaaactct gaatgcgaga ctgcagctgt tcctgttcct gccgcagtgc catccgctct
     2581 tcacgggcgt tagatacgct atcggaaaga gaggcaatgc gtgcttccag ctcctgatgc
     2641 agagcttcca gttcatcaat atcaaaattc ttgccctgac gtttgcagaa atctgccagc
     2701 agacgctcag cttcttgctg ctcacgcaga cgctgttcca gttcgcttaa gcgcatccgc
     2761 aacggctgaa cctgctctgc caggtgacgc tgatcaaccc cttcgcgcaa tagttcgcga
     2821 gcgacatccc acgcctcgtt acgcgccagc gggccgttga ttgccaccac cagctgataa
     2881 gcctgctcaa actggctgtg cgcggtttgc gccatgctca ttttctgctc aagagagagc
     2941 attttttcag tcgcttccag ctctttcgcc tggaaggttt ccagccattc ggcggcgctg
     3001 tcggcggtta agtccggcag atggcacagt tctttggcac gattaagcgc agcaatcgcc
     3061 tggttatact ggatcgcgcg cgtttgctgg acgtccagcg cctgctggta gtcagcaagc
     3121 tggcttttca gctcgtccac ttccagttcg gcagcttccg cgcgagcctc attctcttcc
     3181 tggcgttcga tggcttctgc caccacttca ttttgctctt ccagacggat ctgcagctca
     3241 tcgagatcca cttcgtagcg ttcgatcttc tcctgctgac gcaatgcggt ttgcaccagg
     3301 ttcaggtgat cgctggccgc ctgataatcc gcttccagat cgccttcggc accgttgtgc
     3361 tctgccagct cacgcgccat atcgacgtgt ttgtactgct cagccgccag ttgctgacgc
     3421 gaagtatgta gctcgcgacg aaactccagg gctttgtcga gatggacacg gcgctcgttg
     3481 gcgtgacgca tgtagtccgc cgccacgtag ttggtagctt cgctgatcag atgcttaaac
     3541 aggtcacggt ccgactgggt gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc
     3601 gccgcttcca tgtcctggaa cgctttacgc acgccgctgt tttccggcaa caggtagtcg
     3661 cgcagagaac gggtaatggc actggagatc ccgccataca gcgaagcttc gatcagacga
     3721 tagaatttgc tacggtcaga tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac
     3781 atcagcgagt ggtaatcggt aatggagttg aactgtttaa actgcacgcc ttccatcgcc
     3841 tcgagcttgt ctttcagctc gttaagcggc agcacgcgcg cctggcgttc gttcagggtt
     3901 tcggtcacca gctgtgtcgg ctgcaccgac atcggcagtc cctgaatggc aaatggtttg
     3961 atatccactt tacgatcgcg tccggcaacc tgttgcagac gcacaccgac caccacgcgc
     4021 tggtggcgcg agttaatggt gtcgagcatc gaataacaga cacccgcttt cagcttaccg
     4081 tgcagacctt tatcgcgcga accgctggtg gccccggctt ccgtagtgtt acggaaatgc
     4141 agcagggtca ggtcggggat cagcgccgta acgaacgccg ccatcgtggt ggatttaccc
     4201 gccccgttac cgccggaaag cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa
     4261 aagccgttcc agttaatcag cgtcagtgag cgaaatttac cgcgttcaat cattattctt
     4321 cctctccgct atctggctga ctctcttcgg tttcatcgtt gagttgcaga tgattttcaa
     4381 tcggcattgc ttcgccatca cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag
     4441 cacgcacatc ggctccgaag cggaacaccg attcggtaat gcggaacttg ctgctgtcgt
     4501 ggcccataaa ccacaccatg cctaaacgac gcagacggtt gagcgaagaa cgtactttct
     4561 cctgcaactt ctgacggtca acgtctgaac cggttgaacg gttgttcacc agtttcagca
     4621 gtttcgcttc atcggccagg gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc
     4681 cctcattcgc cagccgttcc gggctgagat agagataaca gaggattttc ccgaccatca
     4741 tatccagttc cgacaagacg gaacgcggga taagagtggt ggaacgtggg cgtaaataga
     4801 agaacccttc cggtgcgcga ataagctcaa cgttataacg cgcgtaaaac tcttccagat
     4861 attcctgaaa atccatcagg aatgcatgat tatccagttc gtcgaggcca atatggcggc
     4921 ctgaacgtaa ggcgctgtcc agcgccggaa ataacggatt cgccagcgcc tgcgccagct
     4981 taaccggcat cacttgttca atatttgtcg atgacatgcg cctgtacctt ggctccgtaa
     5041 tcattaatcg gctgccattt cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga
     5101 cgtaccgcct gatcaataac aatacgcgca acgtcaaagt gacgtgcacg cggatactgt
     5161 gacagatatt cgcgtaccac cagaccaaga tccagcggca cttgtctggt tttgtacacg
     5221 gcaagttgtt cttcgatgat cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat
     5281 tccagatcct caggaagttc cccagtcact tcttcatcgc gcagtgccat ctcttcgtca
     5341 cgcatatcca gcagacgatc ggcattggca taagttagcg cccacggctc atcaaaatag
     5401 gtttgtaccg actgacgtaa ccgctgagca aagacgcggt ttttatccat atcgatcgcg
     5461 gtacgaataa atttgtgtac gtggcggtcg tagccaatcc acaagtcgat ggattgctgg
     5521 ccccaactga taatacgatc gagtttgctc tgcagatcga acaccagacg atcgacgaaa
     5581 tgcagatcgt catgggtcat cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg
     5641 tcgcctgccg cttccagcgt atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc
     5701 aacaattcac agctggaaat cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg
     5761 tccttcacct gctgctgctg ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa
     5821 atttctgcta ccgaatattt cagtggcgca tagacattac ggtgccagtg aaattcatca
     5881 ccgccctctt cggcggcatc tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc
     5941 atagaaagac gcagcgtaga aaactcgcgc tgacggatgt agtagtcagt aatgccgatg
     6001 ccgagcggcg tcagacggta aattgcgttc ccttccgcct gctcgctggt aaagcggttc
     6061 agcagacgtt gacgcaccat gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg
     6121 ctggtttgct caaacgcatc actcacatgg cggaatgcat ccaccagctc gccttcactc
     6181 atctcaccat ccagacgctc gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg
     6241 tctaccggca gcgagatgga gaagtcattt tttctggccc aggcaaccag ttcggggact
     6301 gtctgggaaa attcactcat actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa
     6361 tataacgccc tagggtaata tacggttcct gacggcaata acgcgtttct aattcaagta
     6421 atgcttcata gcagtcgcgc tgctggtgct tctcgcgcag ataatcatga aacacgcgaa
     6481 cgcctgtctt acccataatt tgccaaccag cttcttccag ccacagataa acctgcgccg
     6541 ggtcgcgtgg ataatctggc gaaagcgtcc gttttttctt tttcggcatt cccgcctgca
     6601 cgtaatcaaa attcccggcg accatgttat gcatcaacaa accatgcgca ttgtagaaca
     6661 ttaacgacaa cactctgcct ggacgcaaca ctgaccacag ggtctgcaat acactgcggg
     6721 gatcggccac ccactcgagc accgcatgga acaatatcag atcaacgggc gtttccaaat
     6781 gcgaagcaac atcctgagcg gcgcaatgta taaattgcat gttgtcgctc acaccttttg
     6841 cttctgccgc ctgttttgcg cggtcgatca tctgcgcaga aagatcgcat aaaataacct
     6901 gatgcccgcg ctcggccatt ttgattgcgg tctgcccttc tccgccgcca gcatccagca
     6961 cacgcagttt ttgcgggccc atttccgcca gcacgcgatc gagatcctgc cacagaatag
     7021 cctgtcgaag ctgccctttg gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat
     7081 catcaaaatt gcgatcctgc attgctttct ccaccggttg gctaagacgt tttctgataa
     7141 agacgctatt ttgtcacagc cacggcgaga atgaacccgt ctggtgtaat atcgctggcg
     7201 atcccttgct atatggttaa aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt
     7261 gattggcaat atgttgctgc cacttccgct gatgttgtta attattggcg ctggcctggc
     7321 gctgctgtgg tttagccgtt ttcaaaaaac cgggaagatt tttattagca ttgggtggct
     7381 ggcactcttg ttattgagtt tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag
     7441 cacctatccg acatggaata attcgcaaaa agtggactat atcgtggtgc tcggaggtgg
     7501 ttatacctgg aacccgcagt gggcaccgag ctctaattta atcaataaca gtctgccacg
     7561 cctgaatgag ggtatccgtt tatggcgcga aaatccgggt tcaaaactga tcttcacggg
     7621 aggcgtagca aaaaccaata cggtgagtac agcggaagta ggtaccagag ttgcgcaatc
     7681 gctgggcgta ccgcgcgagc aaattatcac cctggatttg ccaaaagata ccgaagaaga
     7741 agctgcggca gtgaaacagg cgattggtga cgcccctttc ctgctggtga cctccgcatc
     7801 acatctgccg cgggcgatga tcttttttca gcaggaaggg ttaaatccgc taccagcccc
     7861 ggcgaatcag ttggccatcg actcgccgct taatccgtgg gaacgggcaa tcccttctcc
     7921 ggtctggctg atgcatagcg atcgtgttgg ctatgaaacg cttggccgca tctggcagtg
     7981 gctgaaaggt ccgtcaggcg agccacggca agagtgattt tgatgccaga tcgaagttgc
     8041 gccgactaaa tcgtccggta ttaaccagtt gcgcaacttc atcccataac acatacaacc
     8101 aacgccgcca gatgaacgac tccgccactg gcgcgcgttg caggtaactc cagagcaaat
     8161 cttccgccag agaattatcc attagtcgga acagttcgta ttctcgcggt gcccaaagca
     8221 ttagtcccgg cccgaccatc gccagtaact gatcgctgcg cgaatctttc agcatgctgc
     8281 gtaaacagaa gttaccgtga atcaacacac aattgtcgtt aaagccttca aataatgccg
     8341 ggagacattc gcgagtgcga aacaggatcc gcttatcctg catcgtcaga ccggtgttat
     8401 tgaactgatt gagcgtggtc catagcactt caacatgttg ccggtaccat gagggccaga
     8461 aattttcctg agtattgtcg accgcgccga cgcaaccgcg actgtcctga cggtgccagg
     8521 ccagtaaggc ttcaacgatt tggtctttga gctgttccca gcgttctggt gttcgggctg
     8581 gtgcctccac cgaaacacca cgcatccgct ccagtagcag gacatcaggg ccggggtgtt
     8641 cttcatgcgt catcacacca taaatagtcg gcatacggac agtcccactt cttgccagca
     8701 tggtggtttt ccatgccagt tgtcgggctt ttccgggcgt actaaagctt cttgccatta
     8761 acggcattgg gtttccctgg ctgtcataca aagcccacaa cgccgtatcc gctttttcat
     8821 tgacgcactc aatacgactg agtttttcgc ccagtaaatg gcttaattcg gcacgcagct
     8881 gttccattca gattaccctc ataaaagcta ctgctttcat aatgagcgcc aatataaaag
     8941 atgtcacctg aatgagatca attgttgaaa agaaaaattg aggaaaatcg taacggtacg
     9001 acactcctcc caaaattggc tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa
     9061 cgcgctcaag atcttcaggg gtatccacac ctgtgccagg aacttcctga gcaacagcaa
     9121 catggatttt ttcgccgtac cacagaacac gaagctgctc taacatttcg atgtgttcta
     9181 acggacttgc ctgccagttg acgtaacgac ggataaagcc tgcacggtag ccataaatac
     9241 caagatgacg caggaagtta tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat
     9301 cccaaggaat ggtggcacgt gagaagtaca gtgcataccc ttcagcgtcg agaaccactt
     9361 tcaccgcatt cgggttaaac gcttcttccg cattgtggat tggcaccgcc agagtcgcca
     9421 tacccacctg acgctgagcg aggttatcag caacctgacg aatgattgtc gcagggatca
     9481 tcggttcatc accctgcaca ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga
     9541 caacttccac cagacgttct gttcctgact gatgatcggc gcgcgtcata catacttcac
     9601 cgccagcggc ttcaacggcg cgggcaacat cctcatgatc ggttgccacg atgatgcgct
     9661 cggcacctga ttcacgcgcg cgttcaagaa catgaacaat catgggtttg ccgttaatat
     9721 caaccaatgg tttaccgggc agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa
     9781 aactcatgat ttactctcat cagcagtcag cacgcgggct tccgtttcca gcaacactgg
     9841 aatgccatcg cggagcggga aagccaggtt atccagtttg caaataagct cttgtttttc
     9901 ctggttatac caaagttttc cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg
     9961 atccatagtt cctccagatg gatcgggtta tgaatgcata aatcttatca taatcattta
    10021 tgcgacgggg tctatttccc agccagtgcg gcaatcagta aagagacctt gcgggcagcg
    10081 tcctaatgtc acccgcgttg cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa
    10141 ccctttttgc agcattgtcg ttggtttaat gccttcctgt aaccacagag agataacttc
    10201 aaggatgcct gtctggcgat gcattttggc atccattcgc ccaactaatt gcccacgatg
    10261 taataatggc agaacaaaat agccatactg gcgtttcggc gctggggtat agcactccag
    10321 ccggtagcta aaatcaaaaa gctgctctgc gcgtttgcga tcccagacaa caggatcaaa
    10381 aggcgaaagt accgcgctgt gcgttgcagt gagctttccg gccagcgcac gctcgagtag
    10441 cggcagcaaa tcagcatgca gccaaagatt gcccaatttt tcaacatgca cagcaatgat
    10501 ttgctgctgt tcagccctcg cttcgcgcca cgccgccagt gccggacgtt tcagccgata
    10561 gtaatctgcc agccactgtt cgcggaatat tcccaggcta cgcgcactgt tatccagcat
    10621 gatgatttct gcttctgttt gcgaaacgag atcgcgctca tcatcccagt caggcatgac
    10681 tcggtgggtt aaatcataaa cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc
    10741 ggcagtaaat aaaccttcca gatgccgttt atgcggcttc cattcccacc agccgcttgc
    10801 acctttacga ggatgctcaa aatcggctga acgtaccggc cctttatcat gaatatgctg
    10861 aattaactgt gcaatttccg cctcatgttc ctgcatccag gcgtctttgt atttccagcc
    10921 cattttttca ggtgccagca tgcggtggcg aataagacga aagtcgctac gcggcataaa
    10981 gcaggcttca tgcgcccagt attccattaa ttcgccacgc gccagagact catccagcca
    11041 ctgggcagga taatttccca gacgactgaa aagcaccaga tatggactac gggcaacaat
    11101 attgatggta tcgatttgca gcaaggacat gcgggagatc gttgccggaa tatcctccaa
    11161 cgacgctcga cggcggggtt tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc
    11221 atcagcaagg gagaggtgcg gcagcgacat tcatgactcc atcaatcgaa cgctgccgcg
    11281 gcgtaactag ttgccagaag ccagcgaggt tagttgcgta agcagtttcg ctggttcatc
    11341 acctgaaagc tgtgcgtcta caggcaaata ccaccaattt tcttctgcaa aggcccggca
    11401 tttcaccgca tctttttcag tcattaccag cgtttgcccg gcgcttacca acgcactgac
    11461 atccgcatgg ttcaaagact gatgatcggc cagcggtaca catttttccg gttgtacgcc
    11521 acacatcttc agcgtggcaa aaaagcgcgg cggatgccca atccccgcca tcgccactac
    11581 atgttcaagc tgagcaacgt cacaccgcgt accggtacgt aaattcaccg cctgacccgg
    11641 cagcagatgc atggggattt caccgctgcg agggacaccg ccgttgacga ttaccgcatc
    11701 aaccgacttt aagcgccccg ctcgctcacg cattggcccc gccggcaacc accagccatt
    11761 gccaaagcga cgcacaccat caataacgac aatttccaca tcacgcgcca gacggtaatg
    11821 ctgtaaaccg tcgtcggtta cgatgatctg cacatcaggg tgttgcgcca gaatggcttt
    11881 taccgcatca gaacgaacgg gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa
    11941 cacaggttca tcacccgcct gtgctgtggt ggtatctgcc gacaataaca gcggataaga
    12001 ttcagcctta ccaccatatc cccgcgatac gacccccacg cgaataccgc gctgttgcaa
    12061 ctgttccacc agccagacaa cgaccggggt ttttccgttg ccgcctgcgg tgagattacc
    12121 aaccacgaca accggtacgg gggcacgcca ggcgcgcttc agttttagtt tatagcaaag
    12181 acggatcgcg ccactcacca gaccatacaa ccaggagagt ggcagcaata gccgccacaa
    12241 aggggattca ccagaccaga ttttttcgat cattggccaa actgcatttt gtgaagttgc
    12301 gcgtaaacgc cgcggtgctc aagcaaatcg ttatgcgtac cgcgttccac aatgacacca
    12361 tcctcgacga ccacgatttc gtctgccttt tcaatggtag acaagcggtg ggcaatcacc
    12421 agagaggtac ggtttttctg caactcatcc agtgccgcct gaatcgcacg ttcggattcg
    12481 gtatccagag ccgaggtagc ttcatccaga atcagaatcg ggctatcacg cagcaaggct
    12541 cgagcgatag caatacgctg acgctgaccg ccagagagca gcacgccgtt ttcaccaatc
    12601 actgtatcga gaccgttatc catcttattg atgaagtcca tggcgtaggc catacgcgcc
    12661 gcttcttcaa tttgctcacg gctgtactgt tcagtccgtg cgtaagcaat gttgttagca
    12721 accgtatcgt taaacagatg gacattctgc gacaccagag caacctggtt acgtaacgac
    12781 gccagggtat actcgcgcag atcgtgacca tccatcagga tttcaccttc atcaatatcg
    12841 taaaaacgcg tgatcaggct ggcaatggtt gatttacctg aaccagagcg tccaaccaga
    12901 gcaaccgtct tccctgccgg aattttcagg ttgatgttac gcaatgcggg tacgtcacgt
    12961 cccggataag taaaggtgac attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg
    13021 cgcttacctt cgtctttctc ctgctcactg tccagaatgg taaacagcgt ctgacaagcc
    13081 gccataccgc gctggaactg ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt
    13141 gcaatcattg aagagaaaac aacggtaatc gtaccggcag tcaggttatc cataacactt
    13201 gggaagctcg ccgcatacag aacaaacgcc agcgccaaag aggcgatcag ctgaatgatc
    13261 ggatcagaga tggaagaggc tgaaaccatt ttcatcccct gaagacgcat tcggttactg
    13321 actttatcga agcgtttcgt ttccacttcc tgaccaccga aaatcaatac ttctttgtgg
    13381 cccttcagca tttgttctgc actggtggtc acttgcccca tggtgttctg catgttttta
    13441 ctgatgttgc gaaaacgctt cgatactacg cgaatcgcaa tcgaaacaat cggtgccagc
    13501 acaatcaaaa tgatcgacag ttgccaactg taatagaaca tcatgatgaa caggccgatg
    13561 atcgacgcac cttcacgcac aacagtaatc agtgcgccgg aagaagaaga agcaacctgt
    13621 tcggaatcgt aggtgatacg tgacaacagc gtacccgttg actgtttgtc aaagaatgaa
    13681 actggcattc ccatcatgtg accaaacagg cggcgacgca tggtcattac cacctttcct
    13741 gatacccagg agatacagta gctggagaca tagctggtga taccacgtaa aatcatcagt
    13801 ccgatcacca ccagcggcat ccacaccagc acggagcgat ctgttttacc aaagccatca
    13861 tcaagaagtg gcttaaggag cgataacatg aaggtatcgc tggctgcgtt gaggattaac
    13921 gctacgcccg ccacgatcag acccgctttg aaaggcgcaa tggttggcca cagtcggcgg
    13981 aatgtctgcc acgtagagag atctttgtcg ttatgcattc aaaaaaccag catttgttga
    14041 aatagccgca tattctaccc gttatccact ggcacgccaa accactgata ataccaacga
    14101 ggtaaaattt gctctctgag gctgctaatc cgccagcctt gtgctgaaaa attgaccgtc
    14161 acttgaccct gatgtggagt atcaagccat tgatatcctt tctgttgata gcgatgctta
    14221 actttgttag agggcaatcg ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt
    14281 ccattcactc gctgaattaa tggcaacgat gatgaggtat tactgccatg gtgaggtacc
    14341 tgaagcaatg ttgcctgcac ttgctgccag taacggctta gcatcttttg ttcagctggg
    14401 gcttcaatat caccggttag aagaatgcta ttcgtcccgt catcaacctt aaccacacag
    14461 gaatggttat ttcccttatc gttgctacct tgtaaaggcc agtgcgcgct gaaacgcaat
    14521 ccttgccact gccacgcttc gccacgcaca cagggctgat gatgttccca gtttaacgga
    14581 cttctgatcc ataacatcgg ctatatatgc aatattgaat ccagccctcc ccggtgatcc
    14641 agatgttcat ggctcagaat aacgccttcc ggttcaagat tatgccagtg gagccagggg
    14701 ataatcagtt gttgcccact atccccttca ggccaggcca gtcctgtgtc atagagaatc
    14761 gctttgccgt ttctggctat caccattgcc agcccttgcc cgacatcaag catgtacagc
    14821 tgccactcgt caggtcgagg tttttgccac agcggccagc acatcagcaa gcctccagcc
    14881 acacacatta ctggcaacgt tcgccaggcg tttaatcgcc ataccaccag taagaaccat
    14941 ggggaaaatg atagccattg ccaacattca acaatgttga tccacccttc cggcaaactc
    15001 tttaacccct gttccaggat taacggcccg ctaaaatgca caaccatcgc ggcgaggatc
    15061 aacggaaccg tgataaatgt caccaaggga attgcaaata gatttgcaat aaacgaggtc
    15121 agactaatgc catgaaatat gacgatttgc atgggcataa gcaggagtgt gattcccagt
    15181 tgcagatgga tgagggaaac aactgcacgc aataccggcg gcagttgcca ctcaggacag
    15241 ggaaaccact gataccaaaa aatcagtgcc gcgaccgcag cggcagagag ccataaactt
    15301 tgcgagagaa tggcaacagg atccatcagc aaaattgccg ccagacaaca tatccataca
    15361 tcccagccac tccactgtcg cccacttaac ttaagcattc cccacgtagc aagcgccacc
    15421 acggtacgca atgcaggagg ttgcattccc gtcaaccagg cataaaaagc agcacagcag
    15481 attccgccaa ttaatggtat ttgccagtgg atccagcgcc caggcagaaa aatttgtcca
    15541 ctgcgaatga gtccggcagc cagcaacgcc gcaaaagcga tgtgcaatcc cgatatcgcc
    15601 attaaatgcg ccgttccagt atcgcgcatg atatttttga tttctttggg gacggataac
    15661 cgttccccca tacctaaacc aagaataacc gcattccacg gatagggttg cagcgttgtt
    15721 tgtagtgacg ccagatactg tgcacgcagg ctacaattcg gttcaatgac acttgcctgc
    15781 agaaaacggc cggtgagcgg ctgatgctgg gcaatggcat aacgctggct atcaaagccg
    15841 ccatcattaa gttggccatg aactgcacga actttgagtt tcattgacca ttgttgtcca
    15901 gcacaaaccg cttgcggaag ttattcgcca tacagcacaa ggcctggcgc agggaatata
    15961 cgtcgacctt gtagataagt aatttgaccg taatgagtgg tcatgccgtc agttgcagtg
    16021 atctcaacaa ttgcatcctg cgtcgcgcca gttaaggttt ctcctgccca caaaatttgc
    16081 tttgctgata atatgcccca aacgaaaaac agcaacgtca gcgcgatata acggacggtt
    16141 ttaacaggga taaatgccag tacgcaggcg aagagagtca gaaacgcaag ggttaatgtc
    16201 ctaggcaatt ggggcaaaat cagcaacgga aaaattccgc aaattatgca tacgccgact
    16261 gtcgttattt tcatgccccc ctccctgttt atggaaagtg tgcaactttg ttgagcggtt
    16321 gtctggtggg aattttgcgt tccccgaggc acattcagaa gaatttaacg aaagggtgaa
    16381 aactgctcaa aactctgcga ggcagcttcc agttcctgat tagagaattg aaaaaagcac
    16441 ccgacaggtg cttttctcgc gttcaagttt gagtaaaaac ttaaccgtaa atattggcgc
    16501 gatcgcgcag ttctttacca ggtttaaagt gaggaacgta ttttccttcc agttctactt
    16561 tatcgccagt cttcggatta cgtccggtac gtggtgcgcg gtagtgcaaa gagaaactgc
    16621 cgaaaccgcg gatttcaata cgctcgccct gcgcaagagt cgaggccata tgctccagca
    16681 tctcttttac tgcatcttca accgtcttgg cgggaatgtg cgattgctgg gtggcaagtc
    16741 tttctatcaa ttctgacttg gtcatgattc ctccggttcc ttaaaggcaa attaagcggc
    16801 tgctgttgct gcattgatta agggcggccg tagccgccct tagtgcttga ttacaggacg
    16861 aaacctgcaa tctgtcaagt aaactcaaca aacttcggaa taaaaatccc gaagagtcag
    16921 agaattactc gcctttagct gctttgaaag cttcagccat tgcgttgttg gagaagtttg
    16981 catcttcctg tttgttaaca gttgcgattg catctttctc gtcagcttcg tctttcgcac
    17041 gaacagacag gctgattgcg cggtttttac gatcaacgcc ggtgaattta gcttcaactt
    17101 cgtcgccaac gctcagaacc agggtagcgt cttcaacgcg gtcacgggat gcttcagaag
    17161 cacgcaggta accttcaacg ccgtcagcca gttctacggt tgcgccttta gcgtcaactg
    17221 cagttacttt accggttacg atagcgcctt tcttgttcag agcaacccag ttgttgaacg
    17281 gatcttctgc gagctgttta acgcccaggg agatacgttc acgttctgcg tcaacctgca
    17341 gaacaactgc agcgatttcg tcgccttttt tgtattcacg aactgcttct tcgcctgcaa
    17401 cgttccagga gatgtcagac aggtgaacca ggccgtcgat gccgccgtcc aggccgatga
    17461 agataccgaa gtcagtgata gacttgattt taccttcaac acggtcgccc ttgttgtggg
    17521 tttccgcgaa ctgctgccac gggttagctt tgcactgttt cagccctagg gagatacgac
    17581 gacgttcttc gtcgatatcc agaaccataa cttccactac atcgccaacg ttaacaactt
    17641 tggacgggtg gatgtttttg ttggtccaat ccatttcgga aacgtgtacc agaccttcaa
    17701 cgccttcttc gatttcaacg aagcagccgt agtcagtcag gttggtcacg cgaccagtca
    17761 gtttggtacc ttccggataa cgtttagcga tagctaccca cggatcttcg cccagctgtt
    17821 tcaagcccag ggatacacgg gtacgttcgc ggtcgaactt cagcacttta acagtgattt
    17881 cgtcgcccac gttgacgatt tcgctcggat gcttaacgcg tttccaggcc atgtcagtga
    17941 tgtgcagcag gccgtcaacg ccgcccagat caacgaatgc accgtagtca gtgaggttct
    18001 taacgatacc tttaacttcc atgccttcct gcaggttttc cagcagctga tcgcgctctg
    18061 cgctgttttc ggattcgata acggcacgac gagaaacaac aacgttgttg cgcttctgat
    18121 ccagcttgat tactttaaat tcaagctctt tgccttccag gtgcagagtg tcacgcaccg
    18181 gacgaacgtc taccagagaa ccaggcagga acgcacgaat accgttcagc tcaacagtga
    18241 agccgccctt aactttgccg ttgataacac cggtaacagt ttcagcatct tcgtaagctt
    18301 tttccagcgt gatccaggct tcgtgacgtt tagctttctc acgggacagc agagtttcac
    18361 cgaagccgtc ttctactgcg tccagagcaa cgtcaacttc gtcacctacc tggatttcca
    18421 gctcgccctg ggcgtttttg aactgctcag ccgggatggc ggactcagat ttcagaccag
    18481 cgtcaaccag tactacgtct ttgtcgatag caacaacaac gccacgaacg atagaacccg
    18541 ggcgggtttc gatttctttt aaggactctt caaagagttg agcaaaagat tcagtcatgt
    18601 ttaatcttca ggtttatatt taacgtccac ctggctccat gccggatggg gttgtttaac
    18661 atacccgctg tcattccatt gcaacggggg tactgcaaat tcggtcgctt atgcgagagc
    18721 caatttctgg cgcgcgtatt gtagcgcttt ttcaatcact tgctcaatgc ttaaggtggt
    18781 ggaatccaac actaaagcat cggctgccgg aaccagtggc gctaccgctc ggttacgatc
    18841 gcggtcgtcg cgttctttga tctcggccaa aaggcgctcg aagttaacac taaagccctt
    18901 ctcctgcaac tgtagcatgc ggcgatgcgc acgttcttcc gaggaggcgt caaggaaaat
    18961 tttcactggt gcatcaggga ataccaccgt tcccatgtcg cggccatcgg caatcagacc
    19021 tggtaattcg cgaaacgcgc gttggcgacg caataatgct tcacgaacgc gtgggaatgc
    19081 cgcgacttgt gaagccgcat tcgccacttc ctgagtacga atttcgccgc tgacatcttc
    19141 cccttcgagg atcacttcca gattgccatt ggtcgacaca aaacgtacat ccagatggga
    19201 tgccagcggt accagcgcat cttccgacgc aacatcaaca tggtgatgta atgccgccaa
    19261 tgccagtacg cgataaattg cacccgagtc cagcagatgc cattgcaacg cttccgccat
    19321 agccttacac aaggtgcctt tccctgcacc gcttgggcca tcaatggtaa taaccggggc
    19381 aattgccgtc atctttatct ccttaaaaca ggcgtaccgt taacataacc gcgcgcatta
    19441 tacgcgccga cgccacaaat cgttaacgtt gtgtgcaaaa accagttttg tctgtattca
    19501 gatggggatt tattgcgcaa ttatagcggg ctggcagaag accagcccga aagatgacaa
    19561 tgatttactt accagaggcg atacgatcac ggatgtgttg cgcacgctct gttgatggtg
    19621 ggtgagagtc aaacatggac tgggtgcgac cgccatccag gctagccagt ttttcaaagc
    19681 tgccaaccag cccctgggtg ctgatgccac gtttcttcaa cagatcaaaa gagaaatcat
    19741 ctgcttcgga ctctttatca cgggagtatt tagcattaat agcgccttct gcgatatcac
    19801 ccaattgtga gcgggaaagc tgggaagcca cgccgctggt agctgaaatg gcatcgcgtg
    19861 cggcaacgat cgcataagaa gctttcattt cagccagcga gtgacccaac gcgacgtggc
    19921 ccagttcatg gcccagaaca ccttcaattt cgttgtcatt catcatgtcc atcaggccac
    19981 tgtagacacg aacacagccg ttcgccatcg cccatgcgtt gacgtcgctg gtcatataaa
    20041 ccttatagtt gaccggcgtg ccattaatat tgttacccaa tgctttggcg atttttgcca
    20101 gacgtttacc gtacttgctg gatttacttg cgacttgatt gccgctgtcc atttcggcac
    20161 agccctgatt ggcaatcgct tttgcatcgg catcgcttaa tgttgctgct ttataggcat
    20221 ttaaaccgga gctgatagcc atattggtat caataccgtg ggtattttga caccctgtca
    20281 gtaatgctgc agaggtcgca atcgccagca gtaatttagt attcttcatg tgaatcatcc
    20341 gttgattaat catatataaa tattatgtat caacaactta cattgacaag cgacgctacc
    20401 attaatcaaa atgactcaca aggtccgaaa aaaaacgccg acaatgtgcc gtaatgccag
    20461 tcagttaa
//
LOCUS       DAJTXY010000071        19641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTXY010000071 DAJTXY010000000
VERSION     DAJTXY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.071"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..1226
                     /locus_tag="OO566_002582"
     CDS             <1..1226
                     /locus_tag="OO566_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6605818.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1223..2188
                     /gene="yggF"
                     /locus_tag="OO566_002583"
     CDS             1223..2188
                     /gene="yggF"
                     /locus_tag="OO566_002583"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6605819.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2210..2719
                     /gene="fumE"
                     /locus_tag="OO566_002584"
     CDS             2210..2719
                     /gene="fumE"
                     /locus_tag="OO566_002584"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6605820.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2716..3429
                     /locus_tag="OO566_002585"
     CDS             2716..3429
                     /locus_tag="OO566_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6605821.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3401..4078)
                     /locus_tag="OO566_002586"
     CDS             complement(3401..4078)
                     /locus_tag="OO566_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6605822.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4072..4749)
                     /locus_tag="OO566_002587"
     CDS             complement(4072..4749)
                     /locus_tag="OO566_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6605823.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTCSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4737..5120)
                     /locus_tag="OO566_002588"
     CDS             complement(4737..5120)
                     /locus_tag="OO566_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6605824.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNELAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5136..5444)
                     /locus_tag="OO566_002589"
     CDS             complement(5136..5444)
                     /locus_tag="OO566_002589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6605825.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5445..6023)
                     /locus_tag="OO566_002590"
     CDS             complement(5445..6023)
                     /locus_tag="OO566_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6605826.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6046..6477)
                     /locus_tag="OO566_002591"
     CDS             complement(6046..6477)
                     /locus_tag="OO566_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6605827.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELEFSRQPCALSGYDELHISPVK"
     gene            6849..7868
                     /gene="epd"
                     /locus_tag="OO566_002592"
     CDS             6849..7868
                     /gene="epd"
                     /locus_tag="OO566_002592"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6605828.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHSEAHIAAGAKKVLFSHPGSNDLGATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7918..9081
                     /gene="pgk"
                     /locus_tag="OO566_002593"
     CDS             7918..9081
                     /gene="pgk"
                     /locus_tag="OO566_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6605829.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9296..10375
                     /gene="fbaA"
                     /locus_tag="OO566_002594"
     CDS             9296..10375
                     /gene="fbaA"
                     /locus_tag="OO566_002594"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6605830.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10566..11426
                     /gene="mscS"
                     /locus_tag="OO566_002595"
     CDS             10566..11426
                     /gene="mscS"
                     /locus_tag="OO566_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6605831.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11565..12200
                     /gene="argO"
                     /locus_tag="OO566_002596"
     CDS             11565..12200
                     /gene="argO"
                     /locus_tag="OO566_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6605832.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12293..13033
                     /locus_tag="OO566_002597"
     CDS             12293..13033
                     /locus_tag="OO566_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6605833.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13200..14150
                     /locus_tag="OO566_002598"
                     /pseudo
     CDS             13200..14150
                     /locus_tag="OO566_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(14092..15570)
                     /gene="scpC"
                     /locus_tag="OO566_002599"
     CDS             complement(14092..15570)
                     /gene="scpC"
                     /locus_tag="OO566_002599"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR6605834.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15594..16379)
                     /gene="scpB"
                     /locus_tag="OO566_002600"
     CDS             complement(15594..16379)
                     /gene="scpB"
                     /locus_tag="OO566_002600"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6605835.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16390..17385)
                     /gene="meaB"
                     /locus_tag="OO566_002601"
     CDS             complement(16390..17385)
                     /gene="meaB"
                     /locus_tag="OO566_002601"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6605836.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17378..19522)
                     /gene="scpA"
                     /locus_tag="OO566_002602"
     CDS             complement(17378..19522)
                     /gene="scpA"
                     /locus_tag="OO566_002602"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6605837.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
BASE COUNT         4718 a         4910 c         5279 g         4734 t
ORIGIN      
        1 aatttgaact gccagaaatt accgataatg aactgttagt gagtgtaatt tctgacagcg
       61 tctgtttatc gacctggaaa gcggcgttac tcggtagtga acataaacgc gtacccgacg
      121 atttagaaaa tcatccggtc attaccgggc atgaatgtgc cgggattatt gtcgaagtgg
      181 gtaaaaatct cactggcaaa tataaaaaag gccagcgttt tgtattgcaa ccggcgatgg
      241 ggttaccaag cggatattca gcgggctaca gctacgaata ttttggcggc aatgccactt
      301 atatgattat tcccgaaatc gccattaatt tgggctgcgt attaccgtat cacggctctt
      361 attttgctgc ggcgtcgctg gcagagccta tgtgctgcat tattggtgct tatcatgcca
      421 attatcacac cacgcaatat gtttatgagc atcgcatggg cgtcaaacct ggcggcaata
      481 ttgcactgct ggcgtgtgcg ggtccgatgg gcattggcgc tatcgattac gccattaacg
      541 gtggcataca accgtcacgg gtggtggtgg tcgatatcga cgacaaacgc ctggcgcagg
      601 tgcagaagct gctgccggtg gatctggcgg ccagtaaagg cattgagctg gtgtatgtga
      661 ataccaaagg gatgagcgat cctatccaga tgctgcgggc gctgacagga gatgtcgggt
      721 tcgatgacat ttttgtttat gcggcggtgc ctgctgtcgt tgagatggct gacgaattac
      781 tggcggaaga tggctgtctg aacttctttg ccgggctgac ggataaaaac ttcaaagtgc
      841 cgtttaattt ctacaacgtc cattacaaca gcacgcacgt agtcggtaca tccggcggtt
      901 caacggacga catgaaagag gcgattgccc ttagcgccac aggacagtta cagccgtcgt
      961 ttatggtgac ccatattggt ggcctggatg cggtgccaga aaccgtgctc aatctgccgg
     1021 atatccctgg cggtaaaaaa ctcatttata acggcgtgac catgccgctc actgccattg
     1081 ccgattttgc cgaaaaaggc aaaaccgatc cgctgtttaa agagttggcg cggctggttg
     1141 aggaaacgca cggcatctgg aatgaacagg ccgagaaata tctgctggca caatttggcg
     1201 ttgatatcgg ggaggccgcg caatgatgtc cctggcgtgg ccattatttc gcgttacgga
     1261 acaggccgcg ttggctgcct ggccgcaaac cggatgtggc gacaaaaata aaattgatgg
     1321 cctggcggtc accgcgatgc gccaggcatt aaacgacgtc gcttttcgtg gacgagtggt
     1381 tatctgcgaa ggagaaattg accatgcgcc gatgctgtgg attggcgaag aggtgggcaa
     1441 aggcgatggg ccagaagtcg atatcgcggt tgatcccatt gaaggcacgc ggatggtggc
     1501 gatggggcag agcaatgcgc tggcggtcat ggctttcgct cctcgagata gcctgttaca
     1561 tgcgcccgat atgtatatga aaaagctggt tgttaatcga ctggctgccg gggcgattga
     1621 tctgtctttg cctctggcgg acaacttgcg taacgtggcg cgggcgttag gtaaaccgct
     1681 ggacaagctg cgcatggtta cgctggataa accgcgtctg agtgccgcga ttgaagaagc
     1741 aacacagctg ggcgtgaagg tttttgccct gccggatggc gatgtcgccg ccagcgtgct
     1801 gacctgctgg caggataatc cttacgatgt gatgtacacc atcggcggtg cgccagaagg
     1861 cgtgatttct gcctgtgccg ttaaagcgtt aggcggggat atgcaggccg agctgattga
     1921 cttttgtcag gctaaagggg attacacgga aaatcggcag attgcagagc aggagcgcaa
     1981 gcgttgtaag gcaatgggcg ttgacgttaa ccgtgtgtac tcgctcgatg aactggtgag
     2041 ggggaacgat atcctcttta gcgctacggg cgtgacgggc ggcgagttgg tgaacggtat
     2101 ccaacagacg gcgaatgggg tgcggacgca gacattactg atcggcggcg cggaccaaac
     2161 gtgtaatata atagactccc tgcattgatg gtgatctatc ggaagaccaa tggcgactct
     2221 gacagaagat gatgtgcttg agcaactgga tgcacaggac aatttatttt catttatgaa
     2281 aactgcgcat tctattttgc tccaggggat acgccagttt ctgccgtcgc tgtttgtcga
     2341 taacgatgaa gagatcgtcg aatatgcagt gaagccgtta ctcgcccaaa gcggcccgct
     2401 tgacgatatt gatgttgcgc tgcgtttgat ttatgcgctg gggaaaatgg ataaatggct
     2461 gtatgccgat atcacgcact tttcccagta ctggcattac ctgaacgaac aggatgaaac
     2521 gcccggattt gccgatgata taacctggga ttttatcagc aatgtcaaca gtataactcg
     2581 caatgcgacg ctctacgatg cgttaaaagc gatgaagttc gccgatttcg ccgtctggtc
     2641 agaggcgcgt tttagcggaa tggtcaaaac ggcgctgacg ctggcagtaa cgaccacctt
     2701 aaaggaatta acgccgtgaa aattgaatta acggtgaatg ggctgaacgt gcaagcgcag
     2761 taccatgatg aagagataga acgcgtgcat aaaccgttgc tgcgtatgct ggcggcctta
     2821 cagacggtaa atccgcagcg gcgtacggtg gtttttcttt gcgctccgcc ggggacgggt
     2881 aaatctaccc taaccacctt ctgggaatat ctcgcgcaac aagacccgga gctgcccgct
     2941 attcagacgc tcccgatgga tggttttcac cattacaata gctggctgga cgcgcatcaa
     3001 ttgcgcccct tcaaaggcgc accagagaca tttgacgttg cgaaactggc ggaaaatctg
     3061 cgccaggtgg tggaagggga ttgtacgtgg ccgcagtacg atcgacaaaa gcatgatcct
     3121 gttgaagatg cgttgcacgt taccgcacca ctcgtcatcg tcgaaggaaa ctggttgttg
     3181 ctggacgacg agaagtggtg ccagcttgcg caattttgtg atttctcgat ctttatcaac
     3241 gcgccagctg ctgcgttgcg ggagcgtctg gtggggcgca agctggcggg aggggtatcg
     3301 ctggcagatg cagaagcctt ttatgaccgt accgacgggc cgaatgttcg ccgggtgctg
     3361 gaagagagtc tgcctgcaaa tttgacctta atgatgaccg ctacgggtga atatcgtctg
     3421 gtggattaac gttcctgatt acaccatctt ccagtcgcac cacacgagag aaatgacgcc
     3481 gggtaaatgc ggcgtcatga ctgattgcta ccacgctcgt gcctcgctgg cggcacttct
     3541 ccagccagct ttcaaacacg ctcaaccaat tttcgtcaaa atcccgactc ggctcgtcca
     3601 gcagtaatag tggcggagag agtgcttcca ggcaggcgac ggcgaccatc cgtcgctgcg
     3661 cactatgcag atccaacggg tgcgagttgg cagtgtctgc taactgacaa cattgcaggg
     3721 cagcatgggt acgttgagtg atttcatccg ccgggcattt ttgcagtttc aaaccaaatg
     3781 cgatctcgtc agcgacggtg ctgtgaaaaa gttggttttc cgcttcctga aacagtacgc
     3841 caacctttgc cgcgcgctgg cggtttttca ggtttttcat cgcctgttgt tgcagcataa
     3901 ccgtaccggc tgtgggcgta agaagacctg ccattactcg taacagcgtc gatttccctg
     3961 cgccattgtc accggtcagc gccaaccatt caccttgttt gagttgcaga gaaatgtcgc
     4021 aaagacaatc cgttgccgcg ccaggccagc gataagatat cttatttaac gttaacatag
     4081 tggctggagc gctccgtttt gcaattgcca cgcttgctgg cagtagttca ggaaaggtgt
     4141 gcgatgacgt tcaaaaagaa taatcagcga accacgttca aacgcccagt gttgtaagcg
     4201 ctgcaacagc atttcgctgg cctgcggcgt caggcggcta aaggcttcat caagaattaa
     4261 gagctttggc tgcatcgcga tggcgcaggc gatgaccacg cgctgtgtct ctccaccgga
     4321 gagtgttgcc gggtgacgat ggcgcaacgg ctggcactcg gtcagagtca gggcggcatc
     4381 aatccgcgcc ataatctctg cttcagcaag acacagattt tctggcccaa aggcaacttc
     4441 ttcttccacg ctacaggtac agccggagag ctgcaaatag ggcgattgct ggacgagctg
     4501 gatggtggcg gattgttcat taagcgggag atgcccaatg ggcgtaccga ggagcgtccc
     4561 cgtgcctgtt atctctcttg gcagaaagtc cggataccag cccgccatca gctgtgccag
     4621 cgtacttttg ccactgccgt tgtcaccaaa gatggcgacc atccccggtt tgacatagtg
     4681 aaagtcataa cagaacggag gatgcgttga gtgtgtcggg caataacgga actgttctaa
     4741 cgtaaccata tccacgctcc gaattctgtc agcatcagca agatcatggt gtaacgcgct
     4801 acgcgttgta gggtgctatc ggctggtgcc cacaacgttg ttcgtcggtt gtggatccga
     4861 aatgcccgca tatccagcgc cgcaccacgg acagccagct cgttaagcgc attgtgtgtt
     4921 agcggaataa ttaacgcggg catagcacgt aaacgctgat accagccctc atccagcggc
     4981 acgccgcggg cacgctgcgc ttcatgaata atcgctaatt gccgttttaa ctgctcaacg
     5041 accaataacg gcccggcaaa cagataagcg acgcctggcg gcaggcgaga agcaaacaga
     5101 gcgcgaataa aacattgcac ggggacgtac tgcattcaca actgtgaggt tgaaacaatc
     5161 gccagaatca gcaaccagag cgtaatagca tgcgaccacc gttctggcga gcgtggcgtg
     5221 ccgcttagcc attctggtag ccagccaccg tgtaccagcc acagcccggc tcccagcgag
     5281 aacatcagcc agacgacata ttttgcccgc cgccgcgtgg cctttagcgc aataaggcag
     5341 aaaaaggtcg ccgcgctata gatcggcaaa atggtctgcg cgggcaggat aagcgtggtg
     5401 caggccgcta atgcccacag tgttaatgag gtaaatggat gcatttagcg cacggcagcc
     5461 attgccggaa aatggcgcgt ggtgcgaatc ggcagctggc gcagtaaaag ccagacaatc
     5521 accgctgtaa gaattttatc gaccaggttc gcgcctatca ccgtaatcgc caccgactcc
     5581 accaggtttt gccccatcga gtgcatccag gcaacgaaca aatcggctcc gctaccggtg
     5641 acaccgccga acagcgcagt acgcagcggt acagcgacga ccgttaccgc aagcgtaata
     5701 atcacgccgc tgacaacgac ttttggcagg gtgcgaaacc aacccgctcg cgccagccaa
     5761 ccggagacca ggccgatgac catcgcgacg ggtgcaaatg cggcggcgat cgggtcggtc
     5821 agcaatcccc agagtaaatt ggtcagtaag ccggtcagca ttccgatcac cggaccaagc
     5881 agtaccgcgc taataagtgt gccgatggag tcgagaaata taggtaattt gaccatgctg
     5941 gccagttgcc cgccgatcat attgatggcg atagatataa cgatcaacac cagtgcctga
     6001 ctggaaaagt ggctacgcgc cataagaaaa cctctttgaa agtagttatt taacggggga
     6061 aatatgcaac tcgtcgtaac cagaaagcgc gcaaggttgg cggctaaact ccagttcctc
     6121 aaggctaccg atcaccagtt caagcgtggt ccgcacggta cagccgggca tcatatgacc
     6181 gccaagcatc gtgccgtgtg gatcggaaac gcagagatga agatgctcac cactctgttc
     6241 cagcgtgccg tttagtgaga tcacttcgaa tgtaccgttc aggagcgtgg tgccttcttg
     6301 cccggcatag cgcaaggcga cgtcagtcag gctaccggta cagcctgcaa tccaggcggc
     6361 atgaagttga tgttgctgtg caaaagcgtg tagctgggaa aaaacttcct gcccggggag
     6421 gaggcgcagt gcgtaaaagc gtgcggagga agaatatggg tagggcaatg tcatcattgg
     6481 agtactccga aactcggacg tattgcggat aatatcgacg ctgcatcagc catattctga
     6541 tataagtcag ggtttgtgac ggcacaacga aaagattttg ctacgcttat tcgtgattct
     6601 gcaaagtgga ataaattcca caggggcggg gctgaaaacg tgacgtaagt cagataattg
     6661 ctttctttga ctggacaatc attcctttta ttccacgttt cgcttatcct agctgaagcg
     6721 tttcagtcga ttaaatgttc gacaattaac caatcagtcg cagtttgcga caggtaaggt
     6781 ttccccggac gatttgctag attactctgt ctggcaaatt tgctcaggga aaaccttgca
     6841 ggagatctat gaccgtacgc gtagcgataa atggcttcgg tcgcatcggg cgtaatgtgg
     6901 ttcgtgcttt gtatgaatcc ggacgccggg cggaaattac cgtggtggca atcaacgaac
     6961 tggcggatgc tgcgggcatg gcgcatttgt tgaaatatga caccagccat ggccgttttg
     7021 catgggaagt acgacaggaa cgcgatcaac tttttgttgg tgatgacgcc atccgcgtat
     7081 tgcatgaacg ttcactgcaa tcgctcccct ggcgtgaact tggcgttgat gtagtcctcg
     7141 actgcaccgg cgtatatggc tcccgcgagc atagcgaagc gcatattgcc gccggggcca
     7201 aaaaagtgct cttttcacat cctggcagta acgatctcgg cgcgaccgtt gtttacggcg
     7261 tcaatcagga tcaacttcgt gcggaacacc gcatcgtttc taacgcttcc tgtaccacga
     7321 attgcataat tcccgtcatc aaattgttag atgatgcgta cggtattgag tccggcactg
     7381 tgaccacaat tcactccgcc atgcacgatc aacaggttat tgatgcatac catcctgacc
     7441 tgcgtcgcac ccgggcagcc agccagtcga tcattccggt cgatactaaa ctggccgctg
     7501 gtatcacacg attttttccg caatttaacg atcgctttga agcgattgcg gtacgtgtgc
     7561 caaccataaa tgtgacggca atcgatttaa gcgtgacggt gaagaaacct gtaaaagcca
     7621 atgaagtcaa cctgttgctg caaaaagcag cacaaggtgc atttcatggt atagttgact
     7681 atacggaatt gccgttggtc tctgtagatt ttaaccacga tccgcacagt gccattgtcg
     7741 atggcaccca aacccgggtc agtggcgcac acctgatcaa aacgttggtc tggtgcgata
     7801 acgaatgggg ctttgctaac cgaatgctcg acacgacgtt agctatggct actgttgctt
     7861 tcaggtaaga cgcaagcagc gtctgcaaaa cttttagaat caacgagagg attcaccatg
     7921 tctgtaatta agatgaccga tctggatctt gctgggaaac gtgtatttat ccgtgcggat
     7981 ctgaacgtac cagtaaaaga cgggaaagta accagcgacg cgcgtatccg tgcttctctg
     8041 ccgaccatcg aactggccct gaaacaaggc gcaaaagtga tggtaacttc ccacctgggt
     8101 cgtcctaccg aaggcgagta caacgaagaa ttctctctgc tgccggttgt taactacctg
     8161 aaagacaaac tgtctaaccc ggttcgtctg gttaaagatt acctcgacgg cgttgacgtt
     8221 gctgaaggtg aactggttgt tctggaaaac gttcgcttca acaaaggcga gaagaaagac
     8281 gacgaaaccc tgtccaaaaa atacgctgca ctgtgtgacg tgttcgtaat ggacgcattc
     8341 ggtactgctc accgcgcgca ggcttctact cacggtatcg gtaaattcgc tgacgttgcg
     8401 tgcgcaggcc cgctgctggc agctgaactg gacgcgctgg gtaaagcact gaaagaacct
     8461 gctcgcccga tggtggctat cgttggtggt tctaaagtat ctaccaaact gaccgttctg
     8521 gactccctgt ctaaaatcgc tgaccagctg attgttggtg gtggtatcgc taacaccttt
     8581 atcgcggcac aaggccacga tgtgggtaaa tccctgtacg aagctgacct ggttgacgaa
     8641 gctaaacgtc tgctgaccac ctgcaacatc ccggttccgt ctgatgttcg cgtagcaacc
     8701 gagttctctg aaactgcacc ggctaccctg aaatctgtta acgatgtgaa agctgacgag
     8761 cagatcctgg atatcggtga tgcttccgct caggaactgg ctgaaatcct gaagaatgcg
     8821 aaaaccattc tgtggaacgg tccggttggc gtgttcgaat tcccgaactt ccgcaaaggt
     8881 actgaaatcg tggctaacgc tatcgcagac agcgaagcgt tctccatcgc tggcggcggc
     8941 gacactctgg cagcaatcga cctgttcggc attgctgaca aaatctccta catctccact
     9001 ggcggcggcg cattcctcga attcgtggaa ggtaaagtac tgcctgcagt agcgatgctc
     9061 gaagagcgcg ctaagaagta aaaaatcaca gggcagggaa acctgccctt gtttcagcgc
     9121 gcttttagag cacgcatttt ttcatactct aaataattcg agttgcagga aggcgacaag
     9181 agagtgaatc cccaggagct tacataagta agtgactggg gtgagcgaac gcagacgcag
     9241 cacatgcaac ttgaagtatt atgagtataa ggcccgacga tacaggacaa gagacatgtc
     9301 taagattttt gatttcgtaa aacctggcgt aatcactggt gatgacgtac agaaagtttt
     9361 ccaggtagca aaagaaaaca acttcgcact gccagcagta aactgcgtcg gtactgactc
     9421 catcaacgcc gtactggaaa ctgctgctaa agttaaagcg ccggttatcg ttcagttctc
     9481 caacggtggt gcttccttta tcgctggtaa aggcgtgaaa tctgacgttc cgcagggtgc
     9541 tgctatcctg ggcgcgatct ctggtgcgca tcacgttcac cagatggctg aacattatgg
     9601 tgttccggtt atcctgcaca ctgaccactg cgcgaagaaa ctgctgccgt ggatcgacgg
     9661 tctgctggac gcgggtgaaa aacacttcgc agctaccggt aagccgctgt tctcttctca
     9721 catgatcgac ctgtctgaag aatctctgca agagaacatc gaaatctgct ctaaatacct
     9781 ggagcgtatg tccaaaatcg gcatgactct ggaaatcgaa ctgggttgca ccggtggtga
     9841 agaagacggc gtggacaaca gccacatgga cgcttctgca ctgtacaccc agccggaaga
     9901 cgttgattac gcatacaccg agctgagcaa aatcagcccg cgtttcacca tcgctgcgtc
     9961 cttcggtaac gtacacggtg tttacaagcc gggtaacgtg gttctgactc cgaccatcct
    10021 gcgtgattct caggaatatg tttctaagaa acacaacctg ccgcacaaca gcctgaactt
    10081 cgtattccac ggtggttccg gttctactgc tcaggaaatc aaagactccg taagctacgg
    10141 cgtagtgaaa atgaacatcg ataccgatac ccaatgggca acctgggaag gcgttctgaa
    10201 ctactacaaa gcgaacgaag cttatctgca gggtcagctg ggtaacccga aaggcgaaga
    10261 tcagccgaac aagaaatact acgatccgcg cgtatggctg cgtgccggtc agacttcgat
    10321 gatcgctcgt ctggaaaaag cattccagga actgaacgcg atcgacgttc tgtaagtcat
    10381 tcctttctgc ttatctcaag gcccgctctg cgggtctttt tttcgccaaa agcaaggtga
    10441 ttcagatgag aaatctgtga tctatttggc aaaattatgc tttattgttt acccttgtca
    10501 gactgcccgt cataaggcgg cggagtgtat ttctccattt tgagtcagtt gaaaaggaat
    10561 attgaatgga agatttgaat gttgtcgata gcataaacgg cgcgggaagc tggctggtag
    10621 ctaaccaggc gctgctgcta agttatgcag taaacatcgt ggcggcactc gcgatcatca
    10681 tcgttggttt gattatcgcg cggatgattt ccaacgcggt gaatcgcctg atgatctccc
    10741 gtaaaatcga tgccactgtt gctgattttc tttctgcatt agtccgttac ggtattatcg
    10801 cctttacgct aatcgctgcc ctgggacgcg tgggtgtaca aacagcgtca gtcattgctg
    10861 tactcggtgc cgcaggctta gctgttggtc tggctttgca ggggtcactt tctaacctgg
    10921 ccgctggcgt gttacttgtc atgttccgcc cgttccgtgc cggagaatat gttgacctcg
    10981 gcggcgtagc cggtactgtg ctgagtgtgc agattttctc caccaccatg cgtactgcag
    11041 acggtaaaat tatcgttatt ccgaacggta aaattattgc cggaaatatt attaacttct
    11101 cccgcgagcc agttcgccgt aacgaattta ttattggcgt ggcgtatgat tccgatatcg
    11161 atcaggttaa gcagatcctg accaatatta tccagtctga agatcgcatt ttgaaagatc
    11221 gcgaaatgac tgtgcgcctg aacgaacttg gtgcatcgtc gattaatttc gtggtccgcg
    11281 tctggagcaa cagcggcgat ctgcaaaacg tgtactggga tgtgctggag cgtattaaac
    11341 gtgaatttga tgccgccggt atcagcttcc cgtacccgca aatggatgtg aactttaagc
    11401 gggtgaaaga agacaaagct gcgtaatcaa cgctgaggcc agataatact ctctggcctc
    11461 tctcttatta gttattctga ttgccaatta atattatcaa tttccgctaa taacaatccc
    11521 gcgatatagt ctctgcatca gatacttaat tcggaatatc caacgtgttt tcttattact
    11581 ttcaaggtct tgctcttggg gcagctatga ttctgccgct cggcccacaa aatgcttttg
    11641 tgatgaatca gggcattcgt cgtcagtacc acattatgat tgccttactt tgcgctatca
    11701 gcgatttggt cctgatttgc gcggggattt ttggtggtag cgcgttattg atgcagtcgc
    11761 cgtggttgct agcgctggtc acttggggcg gcgtagtctt cttgctctgg tatggttttg
    11821 gcgcttttaa aacagcgatg agtagtaata ttgagttagc tagtgccgaa gtcctgaagc
    11881 aaggcagatg gaaaattatc gccaccatgt tggcagtgac ctggctgaat ccgcatgttt
    11941 acctggatac ttttgttgta ctgggcagcc ttggcgggca gcttgacgtc gaaccaaaac
    12001 gttggtttgc gctcgggaca attagcgcct ctttcctgtg gttctttggt ctggctattc
    12061 tcgcagcctg gctggcaccg cgcctgcgca cggcaaaatc acagcacatt atcaatctgg
    12121 tagtgggatg tgtcatgtgg tttatagcct tgcagctggc gagagacggt attgctcatg
    12181 cacaagcctt gttcagttag gcgctatctg atggaaaaat aaaacagagg cgctaagctt
    12241 gcctccagag gtcctgaatt ttgggcaagt caattaacac ggagagacta acgtgaagtt
    12301 caaagttatc gccctggcgg cattaatggg tattagcggg atggcagcgc aggctaacga
    12361 attgccggat ggaccgcata ttgtcacctc cggtacggca agtgtggatg cggtgccaga
    12421 cattgccact cttgcgattg aagttaacgt ggccgcgaag gatgccgcta cagccaagaa
    12481 acaggcagat gagcgcgtcg cacaatacat ttccttcctt gaactcaatc agatcgcgaa
    12541 aaaagatatc agctcagcga atttacgcac ccagccagat tatgactatc aggatggtaa
    12601 aagtatcctg aaaggctacc gagctgtgag aacggtggaa gtcacgctcc gtcagttaga
    12661 caaactgaat tccttgctgg atggcgcgct gaaggcgggt cttaacgaaa ttcgttctgt
    12721 gtcgctgggc gtggcgcagc cggatgccta taaagacaaa gcgcgtaagg cagcgattga
    12781 taacgcgatt catcaggcgc aggaactggc gaacggcttt catcgtaaac tggggccggt
    12841 atatagcgtg cgctaccatg tttccaacta tcagcccagc ccaatggtgc ggatgatgaa
    12901 agccgatgcc gcgccggtgt ctgcccagga aacttacgag caggccgcta ttcagtttga
    12961 tgatcaggtc gatgtggtct tccagttaga acctgtggat caacaacccg ctaaaacacc
    13021 tgcagcacaa taatacttgt agcccgacgt attcgtcggg ctaatgcctt tacccgatat
    13081 tgcctccttt ttctgtaatt ttctgtaaaa ccatgacgta atcacctatc tttgctcgcg
    13141 gtttcatcaa ggataggctg actgaaacgg ttaagcggac aaggatgcgg ggagggggga
    13201 tggacatttt tatttcgaaa aagatgcgca atttcatttt attagcgcaa accaataata
    13261 ttgcccgggc ggcagaaaaa atccatatga cggcttcgcc atttggtaaa agcattgccg
    13321 cgctggaaga acaaattggc tatacgctat ttacccgcaa agataataat atcagcctca
    13381 ataaagccgg tcaggaactg tatcaaaaac tgttcccggt ttatcaacga ctttctgcga
    13441 ttgataatga aatccataac tcagggcgtc gttcacgaga aattgtgatt ggcatagata
    13501 atacttatcc gacaattatt ttcgatcagt tgattagtct cggagataag tatgaaggcg
    13561 tgaccgccca gccagttgag ttcagcgaaa acggcgttat cgataatctt tttgaccgtc
    13621 agttagattt tattatctcc ccacagcatg tatctgcacg agttcaggaa ctggaaaacc
    13681 tgactatcag tgaattacca ccgctgtgat tgggctttct cgtttctcgc cgttataaag
    13741 agcgtcaaga gcaagagctg cttcaggagc tcccctggct acaaatgcgc tttcaaaacc
    13801 gggctaactt cgaagcaatg attgatgcga atatgcgacc ttgtgggatc aatcccacca
    13861 ttatttatcg tccctacagt tttatggcca aaatcagcgc cgtggagcgc ggacattttc
    13921 tgacggtgat cccgcatttt gcctggtgac tggtgaatcc ggcaacgctg aaatattttg
    13981 atgcgccgca caggccgatg tatatgcagg aatatcttta ttcaatcaga aatcatcgtt
    14041 ataccgccac gatcttcagc atattgctga agatcgtgac gggacaaacc attaacccag
    14101 catcgagccg gttgcaatta aattacggtg taagtcgaag acgtggctaa gatcgtggtg
    14161 aatatgtccg ccgggcgcat tttccagata gcgatgcaga taatcccgat acataggatg
    14221 tgcacaatta tcaatgatag tgcgggcgcg ttgaagcggg gaaagaccgc gcagatcggc
    14281 gatcccttgt tcggtgatga tcactttgac gctgtgttcg ctgtgatcaa catggctgca
    14341 cattggcacg acggttgaga tcttgccttc tttagcaatc gacggagcca taaagatcga
    14401 cagatacgcg ttgcgttcaa aatcaccgct gccgccgatg ccgttcatca gatcgacccc
    14461 ggctacgtgc gttgaatttg catgcccgta aatatcaaac tccaggccga cgttcagagc
    14521 gatgacgccc agacgacgga tgatttccgg gttattggaa atctcctgcg gacgcaacac
    14581 aatgcggctg gcaaagtaat ccatattgtc gtaaatcttg cgcagggaat cggccgagat
    14641 tgtcaggctg gaggcgctgg ccccgctgat tttgccggtt tccagtaaat gcaccaccga
    14701 ttcctgtagc acttccgaat acatcatgaa cggaggaatt tccgggtttt cccccagacg
    14761 cgccattacc gcattattga tattgcccac gccactttgc agcggcagaa attccggcgg
    14821 aatacgccca tgcgccattt cctgcaataa gaacgtgacc acgttatcgg caatctgctg
    14881 gcacatggga ttttgcttat ccagcatatt accggcgtcg ggcaagttgg tttccacgac
    14941 ggcgacaatc tttttcggat cgatttgcac atagcgggta ccgacgcgat ccattgcatg
    15001 gaagatcgac acgctattgc gccgcggtgg cgcgccagga atcacaatat ctgccagttc
    15061 tgcaacgcgc ggatcgtgat agtgattaag ttcaatgatc actttcttcg ctcgcagcag
    15121 ccaggtcggc gcattaccga tcccgctggt taaccagact cgaccatccg gtgccagtgc
    15181 cgatgcttca atgacggcaa catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc
    15241 cacttcgctc aaatgtaggt caacgaaact caccgcgccc tgattgatct ttttacgtaa
    15301 actggacgat gtttgatatg gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc
    15361 gtcagcggcg gcgctgattg acgcccccgt cagcaggcga atttgatacg gctttttggc
    15421 ctcatgctgt tcgttagctc tgcgggcaat cgcggcgggt agggctttcg gcgaacccgc
    15481 tggggtaaag ccgctaaatg ccaccatgtc gttatgctgg ataatttctg ccgcttcatc
    15541 ggcggtcatc cttgtccact gagtttccat ttttactcct tcgttcgcag ggattaatga
    15601 ccaacgaaat taggtttacg tttttcgagg aaagcgttca tcccttcctg gtaatcttcg
    15661 ctgtcataaa ccgcgcggcg catcccctga atacgttcaa attcatcgga gttcatggtg
    15721 tgtgcttcgc ccagtacacg cagctcttct ttgataacgg caatggctaa cggcgctttc
    15781 tcagagatgt ggtgcgccat ttgtaaggtg aaatcttcca gttcttccac ttccacaaca
    15841 tggttgagga tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc
    15901 agctctttga caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta
    15961 tacgggacgc cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc
    16021 agatcggaac tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa
    16081 atgatcggtt tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag
    16141 gagagcggat cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact
    16201 ttggatccac ttggcgcccg caaaatgata cagcgaattt ccggccggtt gagatcgctt
    16261 aacgcctgca taagatcatc aataaagact ttacttaagg cattaagttt tcgtccatag
    16321 ttaaactcaa tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata
    16381 aaaattcctt taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt
    16441 gcgcggtgag agcgtattgt ttttgaccgc taaaagcgtc tggcggtaat agcgatcgaa
    16501 atcttcattc gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc
    16561 caccgattgt tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa
    16621 gtcgatgatg gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag
    16681 accccgtggc tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat
    16741 atgccgggca atggcgacat tggtatggtt atcgccatcg tctttattga taacgagcag
    16801 atcagccact tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat
    16861 ttgcaacgag ataaaacagt ccaccatgcg ggcgacttcc gtttccgact gcgcgacgcc
    16921 aaccgtttcg acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg
    16981 tgctcgctga ctggcaccgc ccagatgacc ggaggagggt accgggcgaa taaacgccgc
    17041 ctcggcacgc gccaggtcat tcatgcgggt tttatccccg agaatgctac cgccagtgac
    17101 cgggctgctg ggatcgaccg caataacagc aacctttaat ccctcacgaa tcaacaacat
    17161 gccaaaggct tcaagaaagg tacttttccc cgcgccgggt gtgccggtaa cgcccagtcg
    17221 cagggcgtta ccacagaacg gcataatagc gtcaagcagc tgtgtactta gcgcctgatg
    17281 acgtgggtga cggctttcca ccagcgtcat ggcttgggcg agcgtggcac gctcaccctg
    17341 acgtaagcgg cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca
    17401 gattcagtac gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca
    17461 cgccgcgctc ttgcaggaag gcgtaatcct gcggcggaat gacgccaccc gcgaccacgc
    17521 agatatcttc gcgtccccat tttttcagcg cttcgaccag ttccgggatc agcgttttat
    17581 gaccggcagc cagtgaggat gcgcccgcta cgtgaacgtc gttttctacg gccaggcggg
    17641 cgatctcttc aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat
    17701 aggcgctggc gatcactttc gcgccgcgat cgtgtccatc ctggcccatc ttagcgatca
    17761 gaatgcgcgg gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat
    17821 cgaactcgga ggccgatttc tcagactgat gatagctttg cgcaatcacg ccggtaacac
    17881 actggcttgg caccagataa cggtcgaaag cgacttccag cgcatcggaa atttcaccca
    17941 gggtggcgcg aacgcgagcg gcattaacag cggcagccag caggttttcg ttatgctgtg
    18001 cggcgtgagt cagggcgttc aacgcggcgg ttacggcggc atcatcacgg gcggcgcgaa
    18061 tgcgttccag cgaagcaatt tgctcgttac gcaccatcac gttgtcgatc tcaagtacat
    18121 cggtttcgtc ttcgtgatcc agtttgtact tgttgacacc aacgatgaca cgcttgccct
    18181 ggtcgatcag cgactgttcg cgcgctgagg cctcttcggt cattcgtttt ggcagacctg
    18241 cttcgatcgc tttcgccatg ccaccggctt cgtcgatctg ttggataata gctctggctt
    18301 gtttgacgat ttgatcggtc agcgactcaa tgtaatagga tccggccagt ggatcgacgg
    18361 tgtggcagag ttctgattct tcctggatga tgatctgggt gttgcgggca atgcgtgctg
    18421 agaaatcggt aggcaaacca agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac
    18481 cgcccagcgt cgcagccagt gcttcaatgg tggtgcggat aacgttgtta tacggatcct
    18541 gttcagtcag gctccagcct gaggtctggc agtgggtacg cagcgccagt gatttcggat
    18601 cctgtgcgcc aaatccactg accgcttcgc tccataaata acgtgccgca cgcaacatgg
    18661 cgacgttcat aaacagatcc atgccgatac cgaagaataa cgacaggcga ggagcgaagt
    18721 catccatttt cagtccggca gagattgctg ctttgatgta ctcaatccca tcggcgagcg
    18781 taaatgctac ctgctgcacg cagttggcac ccgcttcccc catgtggtaa ccgctgatac
    18841 tgatggtatt aaatcgcggc atgttgccgg aacaccaggc gatgatgtcg gcgataatgc
    18901 gcattgacgg ttttggtggg taaatatagg tgttgcggca gaggtactct ttgagaatat
    18961 cgttttgaat ggtgccggtc agtttatcag gtgtaacacc ttgctcttct gcggcgacga
    19021 tataaaacgc cagtactggt agcactgcgc cattcatggt catcgaaacc gacattttac
    19081 ccagcgggat ctggtcgaac aggactttca tatcttccac ggtgtcgata gcgacgcccg
    19141 ctttgccgac gtcacccgcc acgcgtgggt tatcggagtc gtagccacgg tgggtggcaa
    19201 ggtcaaacgc aacggaaaga cctttttgcc cggcggccag gttacggcga taaaaagcgt
    19261 tggactcttt tgctgttgaa aaaccagcat actgacggat ggtccacggt tgggcggtat
    19321 acatagtggc acgcgggcca cgaacgtagg gcggcaaacc aggaagggta cctgtcacct
    19381 ccagattatc gagatcggct tcggtataca gcggcttgat ggcgatccct tccgcggttt
    19441 gctgaaccag cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt
    19501 gccactcctg ctcgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt
    19561 gcgcttacgg tgagcttttt acgtgggttt gtcgccagca aaaaaaatgc attcagacgt
    19621 cgttttttgc cgcctgagat t
//
LOCUS       DAJTXY010000072        19559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTXY010000072 DAJTXY010000000
VERSION     DAJTXY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.072"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..304
                     /locus_tag="OO566_002603"
     CDS             <1..304
                     /locus_tag="OO566_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCR6605838.1"
                     /translation="IMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRN
                     KLLELAGKCHSEELWSPIRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(340..1296)
                     /locus_tag="OO566_002604"
     CDS             complement(340..1296)
                     /locus_tag="OO566_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6605839.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1296..2537)
                     /gene="clsB"
                     /locus_tag="OO566_002605"
     CDS             complement(1296..2537)
                     /gene="clsB"
                     /locus_tag="OO566_002605"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6605840.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2534..3295)
                     /locus_tag="OO566_002606"
     CDS             complement(2534..3295)
                     /locus_tag="OO566_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6605841.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3428..3838
                     /locus_tag="OO566_002607"
     CDS             3428..3838
                     /locus_tag="OO566_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6605842.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3800..4906)
                     /locus_tag="OO566_002608"
     CDS             complement(3800..4906)
                     /locus_tag="OO566_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6605843.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4917..6050)
                     /locus_tag="OO566_002609"
     CDS             complement(4917..6050)
                     /locus_tag="OO566_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6605844.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6043..7779)
                     /locus_tag="OO566_002610"
     CDS             complement(6043..7779)
                     /locus_tag="OO566_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6605845.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7772..8767)
                     /gene="hlyD"
                     /locus_tag="OO566_002611"
     CDS             complement(7772..8767)
                     /gene="hlyD"
                     /locus_tag="OO566_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6605846.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8770..9441)
                     /gene="cecR"
                     /locus_tag="OO566_002612"
     CDS             complement(8770..9441)
                     /gene="cecR"
                     /locus_tag="OO566_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6605847.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9670..10857
                     /gene="rhlE"
                     /locus_tag="OO566_002613"
                     /pseudo
     CDS             9670..10857
                     /gene="rhlE"
                     /locus_tag="OO566_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11064..11546)
                     /gene="ybiA"
                     /locus_tag="OO566_002614"
     CDS             complement(11064..11546)
                     /gene="ybiA"
                     /locus_tag="OO566_002614"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6605848.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            11666..13816
                     /gene="dinG"
                     /locus_tag="OO566_002615"
     CDS             11666..13816
                     /gene="dinG"
                     /locus_tag="OO566_002615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6605849.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13844..14806
                     /gene="ybiB"
                     /locus_tag="OO566_002616"
     CDS             13844..14806
                     /gene="ybiB"
                     /locus_tag="OO566_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6605850.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            14947..16032
                     /gene="hcxB"
                     /locus_tag="OO566_002617"
     CDS             14947..16032
                     /gene="hcxB"
                     /locus_tag="OO566_002617"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6605851.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHKETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(16261..16521)
                     /locus_tag="OO566_002618"
     CDS             complement(16261..16521)
                     /locus_tag="OO566_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6605852.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16786..17052)
                     /gene="ybiI"
                     /locus_tag="OO566_002619"
     CDS             complement(16786..17052)
                     /gene="ybiI"
                     /locus_tag="OO566_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6605853.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17126..17803)
                     /gene="ybiX"
                     /locus_tag="OO566_002620"
     CDS             complement(17126..17803)
                     /gene="ybiX"
                     /locus_tag="OO566_002620"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6605854.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(17845..19559)
                     /locus_tag="OO566_002621"
                     /pseudo
     CDS             complement(17845..19559)
                     /locus_tag="OO566_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4541 a         5142 c         5284 g         4592 t
ORIGIN      
        1 cattatgatg acgttgacag ggttggccct ggtaatcatc gtgaatgcgg cattaatgag
       61 tgaacggccc atttgggtaa taagttgctt aatgattgtg ttatggccag gcattatctc
      121 gcatggacga aataagctcc ttgaactggc aggaaaatgc catagtgaag agttgtggag
      181 tccgattcgt tgcgctttta caggtgcatt aacactctat tactatttta tcggcttctt
      241 tgggatactt gccgcgatag ctataacgct tgtctggcaa aggcatacgc gtttttttca
      301 ttagtcgtaa atgcagatac taaagaactt aactaaactt cacatcgccg cttcattttt
      361 cgcccgtagc ttcttcgctt ggctttccag tagcaggtaa cagatcagcg ccagcagcag
      421 cgggataaag taatacagga cgcgataagc gagcagggcg gcgataatgc tccctttcga
      481 ggtatgctcc ccagccagta gtgcgataaa caccgcttcc agcacaccga tccccgccgg
      541 aatatgcacg atgacgccag caatactact aaccagtaac acgcccagta caaagaaata
      601 gttcacgctt tgaccaagta acagccagat aatcgccccc attaccatcc agttaacgct
      661 ggaaatcagc atctgagcga gggcgaattt ccatgaaggc agcaccagct tttgcccttt
      721 gatggtcata tggcggtgct tcgcgaaagc gcaaaaccac aaataaaccg cgataatcat
      781 cagtaagcca atgccgagaa tacgcagcgt agtttgatcg acataccagt gatccggcaa
      841 ctccaccacg cctgcggtaa agataatccc tgccagtaaa atatagccca gccagttggt
      901 ggtaatactg agcgaggaaa tccgcgtaat agtgctgccc ggtaacccta gtcgggagta
      961 caaacgatag cgcataccaa tgccgccgac ccaggtactg agcgtcaggt tgaaggcgta
     1021 gcagataaac gacaccagca tcacctggcg cttcgccagt ttgtgaccgc agtaaaagcg
     1081 ggcgagcagg tcatagcagc cgtaaatcag atagctgacg accaccagcc cgaccgcact
     1141 aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc agtccacttt
     1201 tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg tgaggatctt
     1261 ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc cgtgttttca
     1321 gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg ggggatcaac ctgcgtcaga
     1381 cgtggtgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa aaagtggaac
     1441 gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt ttcatccacc
     1501 tgctgacaat ctgcggcaat aatgccgttc agattatcgc gcagcgtctg gttaaaatga
     1561 cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg atcgagatta
     1621 ctggacccta ctgtcgccca gtgatcgtcc atcaatgcca ctttgccgtg gagcgggcgg
     1681 cggcggtact caaaaacctg aacgccgcct ttaaccagat agttatacag caagcgcgca
     1741 ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat ccgcaccccg
     1801 cgccgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa gtaggcgttg
     1861 gcgataatca cttcccgccg cgcctgagtg agcattttca gataatggcg ttcaatatca
     1921 tcgcgatgtt cttcgttatc gcgccagacc agcaatacct gcgcttctcc cggctggcgg
     1981 ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg tccaggcagg
     2041 ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg taccgcgtaa
     2101 tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt cagcccgcca
     2161 ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg aaacacattg
     2221 gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac gccagctgcc
     2281 gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc cagcaagact
     2341 tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg tttgccgacg
     2401 ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc gccaatcgcc
     2461 ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt attgccttcg
     2521 cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca gaaaggtgtc
     2581 gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg acgtagatcc
     2641 tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt ccgtgggcgc
     2701 gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct ttttgccgcc
     2761 agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca ttcacccatt
     2821 cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga tgtacgcaca
     2881 tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc acgccgcgct
     2941 tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga taacgcgaca
     3001 gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag gcaaaatcgc
     3061 tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt tccacatgca
     3121 gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg gcgctgacgg
     3181 tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg gtaaagcctt
     3241 tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg ggcataattt
     3301 tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa aaggtgcggt
     3361 cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat tcttcaggag
     3421 agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt gctggcagat
     3481 tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta agactctggt
     3541 tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga tgctggtgtt
     3601 tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag agctgaccac
     3661 gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg tactggaaaa
     3721 caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg tagtgtcttt
     3781 gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac gccgctgacc
     3841 ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata tccagactcg
     3901 catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg ttaatccacg
     3961 tcagattttg cagccacact ggcatgtttt ccaccggcga aacgtaaccg gaaaggagaa
     4021 tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc gagcagagtg
     4081 atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc gtaaagtaga
     4141 acagcgctag cgatccggcg aaggggattt gatacgccca gataccaatc gccagcacaa
     4201 tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg aagatctgcc
     4261 aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt tcgcgggcga
     4321 cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc agcgacggca
     4381 ccacaaacca tttgtagtcg agattcgggt tataccagtt gcgtaccacc agctcgctgt
     4441 tgttgggttt cggttttcct tccagcagct cctgctgata atttttgacg atctgttgca
     4501 ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc aactgcaacg
     4561 gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc accagtagta
     4621 acgccttttg tgtgtcgatg gtcgggcgga tctcctgtgg gcttttcagc agcagcacat
     4681 gtgtaaaggc gctggcgcgg gcaaaacgtt gagtaagttc caccgaatgc tcgccgttat
     4741 cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg aacgggaaca
     4801 ggatcacctg aattagcacg ggtaaaatca gaatcgcgcg ggtttgtggt tcgcgcagca
     4861 acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc ttctccctaa
     4921 tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc cgaagcgatc
     4981 aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa caggctttgc
     5041 agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat caccgcgggc
     5101 atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcagaaa agcggcgttc
     5161 agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag cagccccatt
     5221 cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaatcagcag cgaaccgcga
     5281 tacggcacgc cgagaataaa cactgacacc agcatacaca gcaacatcgc cagcatcccg
     5341 agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat ctccgtagag
     5401 agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga ggtgagaatc
     5461 gcgccgatga ccgtcatgat aatggtcacc gcacccggga taataaagtg ctggcttatc
     5521 gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa agtctgcccg
     5581 ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata cccctgcaca
     5641 aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc ggtggcgttg
     5701 gcgcgctcca tctgttcggc gaaatccacg ggaataacca ccagaccgcg aattttcccc
     5761 gactgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc gatgtagggc
     5821 gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc cagcaaaatc
     5881 ccgacccgca gcttgctgga gtcgaggtta atgccgtaac caaaaataaa cagcagtagc
     5941 agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg ccgcgtctct
     6001 ttgacgcaca gcgcccgcac gcgatgccag gaaaggatcg ggttactcat tgctatgctc
     6061 cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat cgggttgctc
     6121 atcgttagct gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta atttcccacg
     6181 gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg tggtgaccat
     6241 caccgtgacg cctttctcta ccatgctgtt gatgtgcagc caaaattcac ggcgggtgag
     6301 ggggtcaacg ccggaagtcg gttcgtcgag aaagagaata tccggttcat gcatcagcga
     6361 acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg cgtgtgaggc
     6421 aatacttttc aggccaaatg cctcgctcat acgggagatt ttttcgttct gcgcccgacc
     6481 gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca ggttgccgta
     6541 gagcgaaaat ttttgcgcca tatagccgag atgctggcgc gctttaccgg aactctcttt
     6601 cagatccatc cccagcacca gcgcctggcc ggaagtcggc accagcaaac cgcacatcat
     6661 cttaaaggtg gtcgatttac ccgcgccgtt tggccccagc aaaccaaaaa tctccccacg
     6721 tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct tggtcagttc
     6781 tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgcaata ttgcacccag
     6841 cggcgattct gaggttccgg caccgcccag caaatcaata aacgcatctt caaaacgcgg
     6901 cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat cgtctggtgt
     6961 ggcctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt cgctgacctg
     7021 cggcagtttc aaggcgcgtt gcaacagttt gcggttgccc tcgtgtggac tggtcatcag
     7081 aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tctccctgat acagcaactc
     7141 gccttcgttc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat acgaggtact
     7201 ccagaggatt aacatccctt cgcccgccag ctcatgcacc atctgccaca gttcgcgccg
     7261 tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg gttcgcccac
     7321 cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc ccgccaggcg
     7381 tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt gcttacgtgc
     7441 ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca ccgtgagatc
     7501 ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg cgccgtcgtt
     7561 tttgatcgga tcaaagccaa tcaccgtggc actgccgctg tcgggtttca gtaatcccgc
     7621 caacatccgc atcagcgtgg ttttacctgc accgtccggc cccaccaacc ccgtcacata
     7681 accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc ccggaaagcg
     7741 tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg tcaccgaatt
     7801 gtaccgtcac tggcattccc tggcgtaacg catcatcggc gtcggtcacc acaatacgca
     7861 ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg gtaaattcag
     7921 cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga ccatcggtat
     7981 aaagcagcac tttgcgcccc ggctgggcct ggtcaagatt ccgttcatca acataagcgc
     8041 gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca ttgagaaccg
     8101 tgcctggctc taccgcgcgc gttaacagcg tgccatcaga cggtgctatc aacgttgagt
     8161 cctgtaaatt cagctccgcc tgcgccagtt gcgcctgcgc ctgttcgagg ctggctttcg
     8221 cctgagcgat atcctgttca cggttaccgg aacggtactg acgcaattta tcctgtgctg
     8281 atttcagcgt tgcctgtgcc tggtcgcgcg aggagcgggc attttccagg tcatttgccg
     8341 aaatggtgcg gcttttccac agtccttgct ggcggttata gaagttctgc gcatagtcat
     8401 aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca tcgcgatacc
     8461 cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc gcctgcatca
     8521 gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct ttgatagcat
     8581 caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta agattcaccg
     8641 tacgaatatc cacgttgcca tacagcgtca ggccgttatc ttggcggctt tgataccacc
     8701 agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagtccgatc acgacaggtt
     8761 ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga tatgacaggt
     8821 caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc cggtacgtaa
     8881 cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg catgggaatg
     8941 aaggatcatg cgggtgtcat tagcgtcgca gccggtccag gcggcaatca ggcgtgtcag
     9001 gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg ccgtgggaga
     9061 gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg tgagcagctt
     9121 aatcatgttc ctgcaggcgc gaaggatcag ttcacggatg gcagcccgat caggctgtgg
     9181 ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc caataaaatc
     9241 ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt agtaggtgat
     9301 ggcagcgata ttctgcccgg cctgggcggc tatctcgcgg gtggtggcgt tcattccata
     9361 ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt cacccttgat
     9421 tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg attaagatta
     9481 tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca gtcacaggga
     9541 taatttatgc gctgcgtcac aaaaactgct acactccgct ccctcatgac attgtggttt
     9601 ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc ggggcggttc
     9661 ggagtagtta tgtctttcga ttctttgggt ttaagccctg atatcctgcg cgccgttgcc
     9721 gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc ggtgctggaa
     9781 ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg ctttacgctg
     9841 ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg tccggtacgt
     9901 gcgctcattc ttaccccgac ccgtgaactg gcggcacaga ttggcgaaaa cgtccgtgat
     9961 tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gcggtgtcag cattaaccca
    10021 caaatgatga agttgcgcgg cggcgttgat gtgctggtgg caacgccggg acgtttgctg
    10081 gacctggaac atcaaaatgc agtgaagctg gatcaggttg aaatcctcgt cctcgatgaa
    10141 gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt aacaaaactg
    10201 ccagcgaaac gccagaacct gctgttctct gcgaccttct ctgacgatat taaagccctg
    10261 gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa taccgcgtct
    10321 gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga attgctgtcg
    10381 cacatgattg gtaaagggaa ctggcagcag gtgctggtgt ttacccgtac caaacacggc
    10441 gctaaccatc tggctgagca gctcaataaa gatggcatcc gtagtgtggc gatccacggg
    10501 aataaatcgc aaggtgcgcg tacccgtgcg ctggctgatt ttaaatcggg tgacattcgc
    10561 gtactggtgg cgaccgatat cgctgcgcgc ggtctggata tcgaagagtt gccgcacgtc
    10621 gtcaactatg aactgccaaa cgtaccggaa gattatgtcc accgtatcgg gcgtaccggt
    10681 cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca caaactgctg
    10741 cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc gggctatgag
    10801 ccggatccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg tggcggcatc
    10861 aggctgatga acaaacgcaa aactgcctga tgcgctacgc ttatcaggcc tacgtggact
    10921 ctgcaatata ttgaatttgc atgcttttgt aggccggata aggcgtttac gccgcatccg
    10981 gcatttcaca acaagcactt gtcagcaatt tgagaacacg ggaaaaaatt tatctgtttt
    11041 accgcccata agccaccgag acgttacttc tctatagcca attgttcgcg caactccatt
    11101 aaaaggtagc ccagtcgatt cttgccctta ccattaccac cgtctcccca gtaagcatcg
    11161 ttttccgtat gctcaaccag ttttgcgggc gcggttgcca gcaagagcac acgcagttct
    11221 gcatgctgtt cgaatttagc gcgaagtgct ttacgcatca cctgttcttt aaccgactcc
    11281 cagtttttac gaagtggctt ggaacgatta cgccccatgc gagcggcgac catgggggaa
    11341 gaaacccgac ggatctcttc gcggtatttt tcatcaagga atttttgtgc ctgaaaatag
    11401 tgttcggagg tgggccaggt attcccgtca accttgatgg gcctggcggc aaagttagaa
    11461 aaatcaccgt agtcatcgct ggtgctgtag aaatttatga tggtgtcttg catgacgtgt
    11521 tggatccttt gtgctcgaac gggcattaaa ccgcattatg ttggtggtta ttgcgagccg
    11581 ctttccagaa acagaaaaac cattacccct gaaaaccgaa aaatgccaca atattggctg
    11641 tttatacagt atttcaggtt ttctcatggc attaaccgcc gcgcttaaag cgcaaattgc
    11701 cgcctggtat aaggcgcttc aggaacagat ccccgacttt attccccgtg cgccgcagcg
    11761 gcagatgatt gcggacgtcg ccaaaacgct ggccggagaa gaagggcggc atctggcgat
    11821 tgaagccccc accggcgttg ggaaaacgct ctcctatttg atccccggca tcgccattgc
    11881 ccgcgaagag caaaaaacgc tggtggtgag taccgccaac gtggcgttgc aggatcagat
    11941 ttacagcaaa gatttaccgc tgctgaaaaa gatcattccc gatcttaaat tcactgccgc
    12001 tttcgggcgt gggcgctacg tttgtccgcg taatctgacg gcgctcgcca gcactgaacc
    12061 cacgcaacag gatctgctgg cgtttcttga cgacgaactg acgccaaata atcaggaaga
    12121 gcaaaaacgt tgtgcgaagc tgaagggcga tctcgacact tataaatggg atggtctgcg
    12181 cgatcatacg gatatcgcta tagatgatga tctctggcgt cgtttgagta ccgacaaagc
    12241 cagctgcctc aaccgtaact gttactacta tcgtgaatgc ccgttttttg ttactcgtcg
    12301 ggagattcag gaagcggaag tggtggtggc aaaccatgcg ctggtgatgg cggcgatgga
    12361 aagcgaagcc gtactgcctg acccgaaaaa tctgctgctg gtgctggacg aaggtcatca
    12421 cctgccagat gtggcccggg atgcgctgga gatgagcgct gaaatcaccg cgccctggta
    12481 tcgactacag ctggacttgt tcacgaaact ggtcgctacc tgcatggagc agtttcgccc
    12541 gaagaccatc ccaccgctgg cgatccctga acgtttgaat gcgcattgtg aagagttgta
    12601 tgagcttatc gcctcgttaa acaacattct caatctctac atgcctgccg ggcaggaggc
    12661 agagcaccgt tttgcgatgg gcgaactgcc tgatgaattg ctggagatct gccagcggct
    12721 ggcaaaactc accgagatgc tgcgtggtct ggcggagtta tttcttaacg atttaagtga
    12781 gaaaaccggc agccacgata ttgtgcgtct gcatcggttg attttacaga tgaaccgcgc
    12841 gctggggatg tttgaggtgc aaagcaaact ctggcggctg gcgtctctgg cgcaatcttc
    12901 cggtgcgccg gtgaccaaat gggcgacgcg ggaagagcgc gaagggcagc tacacctctg
    12961 gtttcactgc gtgggaatac gtgtcagcga tcagctggaa aggctgctgt ggcgcagtat
    13021 tccgcacatt attgtcacct ccgcaacctt gcgttcgctg aatagttttt cgcgtttgca
    13081 ggagatgagt ggtctgaaag agaaagcggg cgaccgtttt gtggcgctgg attccccctt
    13141 taaccactgc gaacagggca aaattgttat tccccggatg cgctttgagc cttccatcga
    13201 caacgaagag caacacattg ccgaaatggc ggcctttttc cgtgaacagg tggagagcaa
    13261 aaaacatctc ggtatgttgg tactgtttgc cagcggacgg gcgatgcagc gctttctcga
    13321 ctatgtgacg gatttacgcc tgatgttgct ggtgcagggc gatcagccgc gttatcgctt
    13381 agttgaactg caccgcaaac gcgttgccaa cggtgagcgt agcgtgctgg tgggcttaca
    13441 gtcatttgcc gaagggcttg atttgaaagg tgatctgctc agccaggtgc atatccacaa
    13501 aatcgctttt ccgcccatcg acagcccggt ggtgatcacc gaaggggaat ggctgaaaag
    13561 cctcaaccgc tatccgtttg aggtgcaaag cctgccgagc gcctcgttta acctgattca
    13621 gcaggttggg cgactgattc gaagccacgg ttgctggggc gaagtggtta tctacgacaa
    13681 acgcttgctg acaaaaaatt atggcaagcg actactggat gcattaccgg tatttccgat
    13741 agagcaaccg gaagtccctg aaggtatagt taaaaagaaa gaaaaaacga aatccccacg
    13801 ccgtcggcgg cgttaatgat gtgagtcagg taaggagtcg taaatggact atcgcaaaat
    13861 aattaaagag atcgggcgcg ggaaaaacca cgcgcgcgat ttagaccggg atactgcgcg
    13921 cggtctgtat gctcatatgc tcaatggtga agtccctgac cttgagttgg gcggcgtatt
    13981 gattgcgctg cgtatcaaag gggaagggga agcagagatg ctcggctttt acgaagccat
    14041 gcaaaatcac accatcaagc tgacgccgcc tgcaggcaag ccgatgccga ttgtcatccc
    14101 cagttacaac ggcgcgcgta aacaggctaa cctgacgcca ttattggcga ttctgctgca
    14161 taaactcggt tttcctgtgg tggttcacgg agttagcgaa gatccaactc gcgtgctgac
    14221 agaaaccatt ttcgaactga tgggtattac accaacgttg cacggcgggc aggcgcaggc
    14281 gaagctcgac gaacatcaac cggtgtttat gcctgtcggc gcgttttgcc cgccgctgga
    14341 aaaacaactg gcgatgcgct ggcggatggg cgtgcgtaac agtgcacata ccctggcgaa
    14401 actggcgacg ccatttgccg aaggtgaggc actgtgtctt tccagcgttt cgcatccgga
    14461 atacattgga cgcgtcgcga agttctttag cgatatcggc gggtgggcgt tactaatgca
    14521 tggtactgaa ggcgaagtgt atgctaatcc gcagcgctgc ccgcagatca atctcattga
    14581 ccgtgaaggg atgcgggtgc tgtatgaaaa acaggaccct gctggtagcg agttactgcc
    14641 acaagcaaaa gatccggaaa ccacggcgca gtggactgag cgttgcctgg ctggcagcga
    14701 agcgattccc gaatcgctga aaatccagat ggcttgctgc ctggtggcta cgggtgaagc
    14761 ggcaactatc agcgatggcc tggcgcgcgt taatcaggca ttttaatttt tatctcagcc
    14821 ggatgagcca cgtcatccgg cttttacccc accgcaatat gaaattcctg catctttatt
    14881 gaccttcccg cgcccggcgt gcagcataaa aaaacaacaa acacataaca taaacaggag
    14941 ttaaccatgg aaagtggtca tcgctttgat gctcagacgc tgcacagttt tattcaggct
    15001 gtatttcgtc agatgggtag cgaggaacaa gaagcgaaat tagttgccga tcatttaatc
    15061 gcggcaaacc tggcagggca tgattctcat ggtattggca tgatcccaag ctatgtgcgt
    15121 tcctggagtc aggggcatct gcaaattaac catcatgcca aaatcgttaa agaggcgggg
    15181 gcggcagtca cgctcgatgg cgatcgcgca ttcggtcagg tcgcggcaca cgaagcgatg
    15241 gcgctgggga ttgagaaagc gcatcagcac ggtattgctg ccgtggcgct acataactcg
    15301 catcatatcg gccgtatcgg ttactgggcg gagcagtgtt cagcggcggg gtttgtctct
    15361 atccactttg ttagcgtggt cggtattcca atggtcgcgc cgttccacgg tcgcgacagc
    15421 cgctttggca ccaatccctt ctgtgtggtt ttccctcgta aagataattt cccgctgttg
    15481 cttgattacg ccaccagcgc cattgcattt ggcaaaaccc gcgtcgcctg gcataaaggc
    15541 gtccccgtgc cgccaggttg cctgattgac gttaacggcg tgccgacgac caatccggcg
    15601 gtaatgcagg agtcgccgtt ggggtcgctg ttgacctttg ccgaacataa aggctacgcc
    15661 cttgcagcga tgtgtgaaat tcttggcggg gcgctttctg gcggtaaaac gacgcataag
    15721 gaaacgttgc aaaccagccc cgatgccatt cttaactgca tgaccactat catcatcaac
    15781 ccggaactgt tcggcgcgcc ggattgtaac gcgcagaccg aagcctttgc cgagtgggtg
    15841 aaagcctcgc cgcatgatga cgataagccg attttgctac cgggcgagtg ggaagtgaac
    15901 acgcgtcgcg aacggcagga gcaggggatt ccattggatg cgggaagctg gcaggccatt
    15961 tgtgatgcgg cgcggcagat tggtatgtcg gaagagacgt tgcaggcttt ctgtcagcag
    16021 ttagccagct aaaaaaaagc ccgtccagtg gcggacgggc aaacaagggt aacataggat
    16081 caatgagggt tagagcatat gcgtctgtcg gcaaacagac agggaaatac ttgtgctgga
    16141 cgtagcgtaa acgcctgatc cgtccaacgg ttcggatttt gtaggcctga taagacgcgg
    16201 tagcgtcgca tcaggcatct gttgccggat gcggcataaa cgccttatcc gtcctacgaa
    16261 ttacttataa attaccgcag taccgcttaa tttattgttg ttggtggcgg aagtgatgct
    16321 gtatccactg gcaccggctg ccgccgcttt ctcagccagt ttcgcttcca gggcatcgag
    16381 ggtggatgcg ccatcggcag aaaccacgcc gattttattc atgttctgtg cctgggatgc
    16441 cgttaccggt tccgccgcga acacgccaaa tgacagagtt gaaagagcca tagcagcaac
    16501 aacagtattg atagttttca taattaatct ctcgcaggtg atttttgtat aagtgacgtt
    16561 gtttcgtcga tgtgatgagt atcacgtttt tttacgagag ataaaatcga agagaattga
    16621 ccgccttgtt caaataaatt gattgatatc taacatactg ataaaccatt tatttgttgt
    16681 acttatggac ttgttttatg ccgcgtggca gtcacagcag cgttaaaggc acggaatgcc
    16741 acgcaaaata caaattatac tcgctatgca tctcgccagt tgtagttaac gtaactggct
    16801 gtctttcgaa cctctgcgat tatatcctgt atacgctggt ttttgtaaat ctttctcctg
    16861 ctgacaatga atacataagc gcacgccagg aatggcttcc cgacgggctt gcgggatggg
    16921 ggcaccgcac tcttcacatt catacaggct ttcgccacgc ggaatttcac cccgagcgcg
    16981 ggcaatcgca tcttcaattg tactgttgat ctgttcgttg acggcgtcat cgttagccca
    17041 agcggatgcc atatcgtcct ccccatatca atgcttgtac agttaagtgt agctaatata
    17101 gggacgaacc tgggcagttc aagcgtcaga tctccgacca ttcccgcagc agattatgat
    17161 aaagattgag cagcgacagg atctcttcat tttcaccata gcggcttttc agcgactgaa
    17221 tatttttgtc cagttcaaac agcatggcgc gctttttatc atcgcggatc atcgactgga
    17281 tccacataaa tgatgccact cgtacgccgc gggttacggg tgtcacgcaa tgcaggctgc
    17341 tggagggata caacaccaga tcacctgccg ggagttttac ccgatgttgt ccaaaggtgt
    17401 cattaacgac cagttcgccg ccgtcgtagc tttgtggatc gcttaaaaac agcgtggcag
    17461 aaaggtcagt gcgcatccag ccgttttgtg gatggctgcg tactgcgcca tccacatgga
    17521 aaccataggt ttcattgttc tgatagcgat taaacagcgg cgtggaaagg gtacgcggca
    17581 aggcagcggc aaagaacaat gcatgttggt taaccgcgtt cagcacctca ttttgcagtg
    17641 cggcgtataa cgcgctgcgg gtgtctactt gctgattgtt cttaacttgt gcaccttgtg
    17701 ctccggtggt gacgcgtcca tccacccatt cggcttgttc cagttgttcg cgaaaatggg
    17761 cgacgtcctg tggcgataac acgccgggaa tgtggtacat catacaagtt ctccaaaaag
    17821 tggggcctgc gccccacatc tgaatcagaa atgcatattg gctgtgagca agaaggttct
    17881 tggctcgccc gggtgataac ggtagccgct cttgttgatt gaggcgacgt aatcggtatc
    17941 aaacaggttg taaacgttta gctggaagtc gagattgcga ttaacccgat accccagttt
    18001 ggcatctgcg acccagtaac cttctgtaaa tgctggcgtt cccaccgcgc cgtctgaacc
    18061 tttatgtata ctgccgatat agcgtgcgcc cgcgccaaca gagatatcat cggttgcctg
    18121 atattggctc cataaggtga aggcgtgctc cggggtatac ggcagcgatg aggaaccatc
    18181 ctgggcaaca tctttgccgt ttttgatggt tgcttttttc tgggtatagc cgccaatcac
    18241 ctgccacgcg ggagtgatat tccctgccac ggatatctca tagccttcga cgcgtttctt
    18301 accgtattgc gagtaagtcc cgtcatcatt ttgctcaact tcattttcga tatcagtacg
    18361 gaacagcgcg gcggtgagca acaggcgttt atccagaacc tgccatttgg tgccaatctc
    18421 gctggtgttg gctttttgcg gtttaaaatc ggtgcggttg gcactgttac cactgccaga
    18481 ctgcgcaagg gcgaagttgt tgccgcccgg aggctgctgg gaaacggcat agttaatata
    18541 gacattgccg ttttccgtca ggtgatacag cgccccggct ttccagttca ccagattgcc
    18601 cgacttggcg gtgtcgacgg tggtgactgg agaacctttt gccacaccag ctgggcaggt
    18661 gatggcaccg cgtccgctgc cgccgcaggc ggtggcactg tcatattcag tatgataatt
    18721 atccagacgg atcccgccgt tcagctcaaa atcacgggtg atttgcagcg tatcgaaggc
    18781 gtaaattgcg aaggtatccg tctgaccatt ggcgtttgcg ccgttgcgcg tcaggccgcc
    18841 gggatgaatt ctgctgtcag gatgataaat atttaccgcc ggtaacgtca ccggattaac
    18901 gccatagtta gtctgcgtct cacgggtaaa ttccacgccg gtactgacat catgaccgat
    18961 agaaccggta tagaacgtcg aggtcaggtt ggtctggttg gtgagaattt tattactcac
    19021 atctttggta ttcgccgtgc gtgactaggt ccagctattc acatcgctgg tgggctgagt
    19081 aatattcgac gccccgccca taatcgccgt catcaggtaa tcctgcttta cgcgcgacca
    19141 acgggtagta ttgcgaatgg tggtgttatc gttgatgtcg tgctcaaaac gcatggtggc
    19201 ggtgtcggtg gtcgaatcgt cgtaatcgga atccgtgccg taaaagttat gagtatcaac
    19261 ttttccggaa tgattcaggg ccgccgctcc cgcagatggg gcagaatagc ccggcaaacc
    19321 gatggtcgga atgccgccgt ctggcgtgtt gtgctgagtg acatgcagat aattaagata
    19381 caaacgattc gctgtaccaa ggccaaaaga gacagaaggg gcgacgccgt aacgctcatt
    19441 tttgactttg tcgcgtccgg catcgtgggt tttttcgccc atcacattca ggcgcaccgc
    19501 ggtagtatca ccaatgacct gattgacgtc cagcgtgccg cggcggaacc aggcgctgc
//
LOCUS       DAJTXY010000073        19520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTXY010000073 DAJTXY010000000
VERSION     DAJTXY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.073"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..175
                     /locus_tag="OO566_002622"
     CDS             <1..175
                     /locus_tag="OO566_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6605855.1"
                     /translation="IRNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQR
                     FGMTPGEYRRKFR"
     gene            complement(347..676)
                     /locus_tag="OO566_002623"
     CDS             complement(347..676)
                     /locus_tag="OO566_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6605856.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(681..866)
                     /locus_tag="OO566_002624"
     CDS             complement(681..866)
                     /locus_tag="OO566_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6605857.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(863..3502)
                     /locus_tag="OO566_002625"
     CDS             complement(863..3502)
                     /locus_tag="OO566_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6605858.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3710..4699
                     /gene="ldhA"
                     /locus_tag="OO566_002626"
     CDS             3710..4699
                     /gene="ldhA"
                     /locus_tag="OO566_002626"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6605859.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4810..5157
                     /gene="hslJ"
                     /locus_tag="OO566_002627"
     CDS             4810..5157
                     /gene="hslJ"
                     /locus_tag="OO566_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6605860.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEISFGEKMMISGSMCNR
                     FSGEGKLSNGELTAKGLAMTRMMCANPQLNELDNTISEMLKEGAQVDLTANQLTLATA
                     KQTLTYKLADLMN"
     gene            complement(5154..5420)
                     /locus_tag="OO566_002628"
     CDS             complement(5154..5420)
                     /locus_tag="OO566_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6605861.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5694..9218
                     /gene="nifJ"
                     /locus_tag="OO566_002629"
     CDS             5694..9218
                     /gene="nifJ"
                     /locus_tag="OO566_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6605862.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9585..10714
                     /gene="ompN"
                     /locus_tag="OO566_002630"
                     /pseudo
     CDS             9585..10714
                     /gene="ompN"
                     /locus_tag="OO566_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            10855..11289
                     /gene="uspF"
                     /locus_tag="OO566_002631"
     CDS             10855..11289
                     /gene="uspF"
                     /locus_tag="OO566_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6605863.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11465..12400
                     /gene="ttcA"
                     /locus_tag="OO566_002632"
     CDS             11465..12400
                     /gene="ttcA"
                     /locus_tag="OO566_002632"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6605864.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12529..13896)
                     /gene="dbpA"
                     /locus_tag="OO566_002633"
     CDS             complement(12529..13896)
                     /gene="dbpA"
                     /locus_tag="OO566_002633"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6605865.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTLALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14374..15357)
                     /gene="zntB"
                     /locus_tag="OO566_002634"
     CDS             complement(14374..15357)
                     /gene="zntB"
                     /locus_tag="OO566_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6605866.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15612..16844
                     /gene="dgcM"
                     /locus_tag="OO566_002635"
                     /pseudo
     CDS             15612..16844
                     /gene="dgcM"
                     /locus_tag="OO566_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(16865..17428)
                     /gene="smrA"
                     /locus_tag="OO566_002636"
     CDS             complement(16865..17428)
                     /gene="smrA"
                     /locus_tag="OO566_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6605867.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17682..19136)
                     /locus_tag="OO566_002637"
     CDS             complement(17682..19136)
                     /locus_tag="OO566_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6605868.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19133..>19520)
                     /locus_tag="OO566_002638"
     CDS             complement(19133..>19520)
                     /locus_tag="OO566_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6605869.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5130 a         4957 c         4591 g         4842 t
ORIGIN      
        1 tattcgtaac cgtcgtctcg atttttgtgc agatgcgatt cgccatgccg cagatgatga
       61 aaaactggca ggcatcggct ttcattgggg attttctgac cagagtcatt tctcaacggt
      121 atttaagcaa cgctttggga tgacgccagg cgagtatcgc cgtaaattcc gctaactaat
      181 tggcgttgca tgtactgcaa cgccaattag ttaaaacaga tatttgacta tgcatttttc
      241 agggcgaaag gcaatttgac aggagttttt gaatattcac cttgtttcag gaaaatagat
      301 caatcgcttt ctcctgtagc gttatcccga tattgcggtt ttgctattaa aactttcgca
      361 cccatttacc gtttatccct tgtacatatt cccccggttt ggcccgagct acgagctttt
      421 gcccggcgag tttcgcgata tcatctacag acacattatt ttgctttgcc cgttgttggt
      481 aactcgcttt acgttcggca ttaatttcgg ttactaattt ctctgtttca gcatccgttt
      541 tcagcgcaac cagataacca taaaatgttt cacctacccg cccctgagtt ctggcttcat
      601 ccagagtcaa tgccattaca ttgctgctta ccagcccaac aagaaacgca caaagaagta
      661 atgttttctt catcatcgcc tcagaaaaga tcgctacgag tttcaagcag ctcttcgaca
      721 tctttgtctg ccttgatgat gatctcatgc tcaattttaa cgttcatatt gatagtgatc
      781 ggttccttag gtgcagcgac ttcaatgcga ggcgtacagc caaccagcat aaaagatgac
      841 gtcaacgcag ccagtaaaat tttcattgtt tttcctcaca ctcttttcct tgcggacagt
      901 catttcccgg cagacgtgcg ttctgctcca accatgcctg gagattatcg ccaaagcgta
      961 aactgcgcca cagcgtaaaa atattctctt catgatggta attaaggttt accgtaccgc
     1021 ttttaccatc aacgcgactg gtacctgtaa tgttggcctg catggttaat aaaccgagat
     1081 tatctaagtt aatttttgtc gatgaccggc taatttccat atagcgcaac cagttaattg
     1141 ctgaacccgc agtcatattg tctttcacca ccgcatctgc ggtatcttta tccagccgca
     1201 aggtcattgg cccgctattt gccagccaac catctttcac tatccatttt tcgttgttta
     1261 gccataacgg caatgcacca ctaaatgccc cggacatggc gaattgtttc ggattgacgg
     1321 cgctaaccag ttcgctggat gagagattat tcagccgcaa cagcgccggg tcatgttgcg
     1381 gcatacgtaa ttgtttcatc agcacgttac cgcctaacac atcgacgcta acatcagtca
     1441 acagcaaggg ttcttcttca gtccacggat aacgcccttg caaatcagcc gtaatatttt
     1501 tcgctgtcac cagattaatc acttcggcaa ttcgcaacgt aacggggccg cgagtcccca
     1561 gatgccaggc tccatcggca aaacggaaag gcaggacaaa atcgacaccg ttaacctgat
     1621 tatctggcat ccaggcacta ccgcctttca acacgccgtg ccctcccgcg cggaatcctt
     1681 gttcaggcgc agcagaaaat gcaacctgag catacagttc accatcgcgt aagttcatct
     1741 tccagtcggg tggcaccagc ggctggaata cagtcagtga ttgttttggc caccaggcgt
     1801 taccgcgcag acgaataccg tcccagcgac catttacccg aaccgggcca atctcaccaa
     1861 catgtaaatc gcctttaaag aggaaatagg tcggatcgcg cccatcgacg ctaaatttta
     1921 aggttgatgg cggtaacaca ctgccgccag tgaacagcgt ttgcccggcg tccagtgaca
     1981 gcgcgccgct gaaggaggga tgctgtgcgt cacgtaccca gacgacgggc ttgtcgagaa
     2041 ttaatcgcgg cttttctacc gtcatcgtac cgtattgtaa ctggtcgaaa ccggtggaga
     2101 gatcggtcag cgtaatcgtg ctgtcatgcc actcaccttt tcctgcgaca tcccaggtgg
     2161 cattcatcgg tgtaaaactc ccttttcccc agtagcgcca ctgccagcgg ccagcgtcag
     2221 ggagaaaatc attcgccagc ccatccatat gcagcacgaa atcgcccagt tcattttcat
     2281 gcgcctgcaa aatggcctgc aaacgtccgt caacaccacg ttgggtgact tttacacccg
     2341 ctaaaggcca gcggatttca tcgatgtcca gcgaatcgat gactcttccc tttgaacgaa
     2401 gtaacgcgcc tggctcaaag gtcagcgttg ggtcagacag acttccactt agctgcgcag
     2461 gtaaacgggc atataaaatg aggtccgcct gtttcgcttc accggtcagc tgcagaggca
     2521 gttgactgtt atccatgctt aattttcctg ggccaaaatt aagcaccgcg ttgcccttac
     2581 ccgcttgccc ctgggtcagc acactcagtc gtccgctgac cagagcgttc tcaagccctg
     2641 cctgccagtt gtcaactttg acacccagtc ggccactcaa aggaaaacct gcatacagcc
     2701 agctccagcg accatcgctt acggtcaatt gttgacgagt aatttgccac ggcaaatcga
     2761 gcaacggatc gccgttatcc cgtgccagca cgatcaattg cccgctattt tcctgccagt
     2821 ccagctcggc atccaccagt gacggttcct gcggcaagtt taacgtcgca gtagcatgcc
     2881 cacttacagg aagtccatcc ggcacgagcg gcatagtaaa ttcccccacc agtttaaccg
     2941 gcggctgatt ttcaaacgcg acgacatcca gttcgctgac tgtaagttgt tgccctttca
     3001 gctggccttg aaatttaact ttttcgccct gataacgcag ttgctggata tcagaggtta
     3061 atgcgagaga gagttttccc tgccattcct gccagggaga aaaaatcagt ttatcgatat
     3121 tgatccaggt gttaggcagc atggactgcc actgcgcgag ggtttttgga gcagctggcg
     3181 attgctccgt ctgcggcaat ttcgccagac aagcagaatc aagttctacc gtgccgacgt
     3241 tcaataacca gcggctggga tgtgaaaggc tggcgttggt gatatgcgca agctgacaat
     3301 ctcccaccag ataacggaga tcgggaatga ttaaaccttt acgcgtaatg cgtgggcttt
     3361 catctaatgc aatacgtgtc ccgagcggta gccagatgtc cgccagcgtg ggaacccaca
     3421 gcccgagcgt catcagcagc gtcaacggca caagaataat cagtaataac agcgcgagaa
     3481 cggctttata tttacccagc atgggtagtt aatatcctga tttcgcgaaa aattaagcat
     3541 tcaatacggg tattgtggca tgtttaaccg ttcagttgaa ggttgcgcct acactaagca
     3601 tagttgttga tgaatttttc aatatcgcca tggctttcaa ttatatttga aattttgtaa
     3661 aatattttta gcagcttaaa tgtgattcaa catcactgga gaaagtctta tgaaactcgc
     3721 cgtttatagc acaaaacagt acgacaagaa gtacctgcaa caggtgaacg agtcctttgg
     3781 ctttgagctg gaattttttg actttctgct gacggaaaaa accgctaaaa ctgccaatgg
     3841 ctgcgaagcg gtatgtattt tcgtaaacga tgacggcagc cgcccggtgc tggaagagct
     3901 gaaaaagcac ggcgttaaat atatcgccct gcgctgtgcc ggtttcaata acgtcgacct
     3961 tgatgcggca aaagaactgg ggctgaaagt agtccgtgtt ccagcctatg atccagaggc
     4021 cgttgctgaa cacgccatcg gtatgatgat gacgctgaac cgccgtattc accgcgcgta
     4081 tcagcgtacc cgtgacgcta acttctctct ggaaggtctg accggcttta ctatgtatgg
     4141 caaaacggca ggcgttatcg gtaccggtaa aatcggtgtg gcgatgctgc gcattctgaa
     4201 aggttttggt atgcgtctgc tggcgttcga tccgtatcca agtgcagcgg cgctggaact
     4261 cggtgtggag tatgtcgatc tgccaaccct gttctctgaa tcagacgtta tctctctgca
     4321 ctgcccgctg acaccggaaa actaccatct gttgaacgaa gccgccttcg atcagatgaa
     4381 aaatggcgtg atgatcgtca ataccagtcg cggtgcattg attgattctc aggcggcaat
     4441 tgaagcgctg aaaaatcaga aaattggttc gttgggtatg gacgtctatg agaacgaacg
     4501 cgatctgttc tttgaagata aatccaacga cgtgatccag gatgacgtat tccgtcgcct
     4561 gtctgcctgc cacaacgtgc tgtttaccgg gcaccaggca ttcctgacag cagaagctct
     4621 gaccagtatt tctcagacta cgctgcaaaa cttaagcaat ctggaaaaag gtgaaacctg
     4681 cccgaacgaa ctggtttaat cttgccgctc ccctgcaatc caggggagct gattcagata
     4741 atccccaatg acctttcatt ctctattctt aaaatagtcc tgagtcagaa actgtaattg
     4801 agaaccacaa tgaagaaagt agccgcgttt gttgcgctaa gcctgctgat ggcgggatgt
     4861 gtaagtaatg acaaaattgc tgttacgcca gaaatcagct ttggtgaaaa aatgatgatt
     4921 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     4981 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5041 aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta
     5101 acgctggcga ccgcgaaaca gacattaact tataagctgg cggatttaat gaattaatag
     5161 ctgccacagc tcccggcggc aagtgactgt tcgctacagc gtttgccgtt gggtaatgca
     5221 cacatcccaa tcgccgtacc atccagttga cgggcaacag aaagcgaacc gccgatcatt
     5281 gcacaatttg cttctccact actggacatc gacgctttta aacttggcgc tacgtgcgcc
     5341 gcagtcgcct gctgaacagg ctcactacta cacgccgaca acaataaagc ggcacaccct
     5401 acccaaaacg ctgctcgcat ctcttcttcc tctgatcttc aagccaaacg acaccgccat
     5461 aaataatagg cagcacagag ggcggcgtcg agagctgtcc tgcgcgttgc cccgcatttt
     5521 tactttttta tggctatttt tttgccctct gtttgatcaa aacattcatt actctgatgt
     5581 gggggacaca aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa ggattactac
     5641 tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt catatgatta
     5701 ctattgacgg taatggcgcg gttgcttcgg tggcgtttcg caccagtgaa gttatcgcca
     5761 tctaccctat tacccccagt tccacgatgg cagaacaggc tgatgcctgg gccggaaacg
     5821 gcttgaagaa cgtttgggga gacacaccac gcgtggttga aatgcagtcg gaagcgggtg
     5881 ctatcgctac cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg tttacgtcat
     5941 cgcagggttt gctgctgatg atcccgacgc tgtacaaact ggcaggcgaa ctgacgccat
     6001 ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc actttctatt tttggcgatc
     6061 attccgacgt tatggcggtg cgccagacgg gttgcgcgat gttgtgtgcg gctaacgtcc
     6121 aggaagcgca agactttgct ctcatttcac acatcgcaac gctgaagagc cgcgtgccat
     6181 ttattcattt ctttgatggt tttcgcacgt cccacgaaat caataaaatt gtcccgctgg
     6241 ccgatgacac tattcttgat ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg
     6301 cgctcaaccc ggaacatccg gtgatccgcg gtacgtccgc caatcctgac acttatttcc
     6361 agtctcgcga agccaccaac ccctggtaca acgcggtcta tgaccatgtt gaacaggcga
     6421 tgaatgattt cgctgccgcg acaggtcgtc agtatcagcc gtttgagtat tacgggcatc
     6481 cgcaagcgga acgggtgatt atcctgatgg gctctgccat tggcacctgt gaagaagtgg
     6541 ttgatgaatt gctaacccgt ggcgaaaaag ttggcgtgct gaaagttcgt ctgtaccgtc
     6601 ccttctccgc caaacacttg ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac
     6661 tggacagaac caaagaaccc ggtgcccagg cagaaccgct ctatctggat gtgatgaccg
     6721 cactggcagg agcctttaat aatggtgagc gcgaaaccct gccccgtgtc attggtgggc
     6781 gctatggtct ttcatccaaa gaatttggcc cggactgtgt actggcggta tttgccgagc
     6841 tcaacgcggc taaacctaaa gcacgcttta cggttggtat ttacgatgat gtgaccaatc
     6901 tgtcactgcc gttgccggaa aacactctgc caaactcggc gaaacttgaa gccttgtttt
     6961 atggccttgg tagtgatggc agcgtttccg caaccaaaaa caatatcaag attatcggta
     7021 attccacgcc gtggtacgca cagggctatt ttgtttacga ctccaaaaaa gcgggcggcc
     7081 tgacggtttc tcaccttcgc gtgagcgaac agccgattcg ttccgcttat ctcatttccc
     7141 aggctgattt tgttggctgc caccagttgc agtttatcga taaatatcag atggctgagc
     7201 gtttaaaacc tagcggcatt ttcctgctta atacgccgta cagcgcagat gaagtgtggt
     7261 cgcgcttgcc gcaggaagtt caggccgtgt taaaccagaa aaaagcgcgc ttctatgtga
     7321 ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc
     7381 agatggcttt tttccatctg acgcaaattc tgcctggcga tagcgccctc gcagaattgc
     7441 agggtgcgat tgccaaaagt tacagtagca aaggtcagga tctggtggag cgcaactggc
     7501 aggctctggc gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc
     7561 acagcgccaa tcgaccgcca gtggtttccg atgccgcccc tgatttcgtg aaaaccgtaa
     7621 ccgcggcgat gctcgccggg cttggtgatg ccctccccgt ttcggcgctg ccgccagacg
     7681 gcacctggcc gatgggcacc actcgctggg aaaaacgcaa tatcgccgaa gagatcccca
     7741 tctggaaaga ggaactctgt acccaatgta accactgcgt cgccgcttgc ccacactcag
     7801 ctattcgcgc aaaagtggta ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt
     7861 cgctagatgt gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag gtggcaccgg
     7921 aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc
     7981 cagagattaa agccatcaat atgatgtctc gccaggaaca tgtcgaagaa gagaaaatca
     8041 attacgattt cttcctcaac ctgccagaaa tcgaccgtag caaactggaa cgtattgata
     8101 ttcgtacatc gcagctgatt acaccgctgt ttgaatattc aggtgcttgc cccggttgtg
     8161 gcgagacgcc gtatattaaa ttgctgactc agctctatgg tgaccggatg ttgatcgcta
     8221 acgccaccgg ctgttcttca atttatggcg gtaacctgcc ctctacaccg tataccaccg
     8281 atgccaacgg tcgtgggccg gcatgggcga actctctatt tgaagataat gccgaatttg
     8341 gccttggttt ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc
     8401 aatttgccga taaaatcccg gcggaattac tgacggcgtt gaaatcagac gctacgccag
     8461 aggttcgtcg agaacaggtc gcagctttac gccagcaact caacgatgtt gccgaagcac
     8521 atgaactgct gcgtgacgca gatgcactgg tggaaaaatc aatctggctg attggtggcg
     8581 acggctgggc ttacgatatc ggctttggcg gtctggatca tgtattgagt ttgacggaaa
     8641 acgtcaacat tctggtgctg gatacgcaat gctattccaa caccggtggt caggcgtcga
     8701 aagcgacacc gctgggtgca gtaactaagt tcggcgagca cggcaaacgt aaagcgcgta
     8761 aagatcttgg cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag atttctctcg
     8821 gcgcgcagct gaaccagacg gtgaaagcga ttcaggaagc ggaagcgtat ccgggaccat
     8881 cgctgatcat tgcttatagc ccgtgtgaag agcatggtta cgatctggct ctcagccacg
     8941 accagatgcg ccaactcact gctaccggct tctggccgct atatcgcttt gatccgcgtc
     9001 gtgccgatga aggcaaactg ccgctggcgc tggattcacg cccgccgtca gaagcactgg
     9061 aagaaacgtt acttcacgag cagcgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg
     9121 cagaacagtt atggaaagat gctgcagctg atttgcaaaa acgctatgac ttcctagcac
     9181 aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat gctctggata aggattatcc
     9241 aattctaaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc
     9301 agtggcaatg caatggccca acagaaaata aaatctgtga caataaaggc atataacccg
     9361 cgcagaataa cgtatagaaa attaaaagta taatttttat tttgtatgta ttatcttcga
     9421 tacattcacc agacttatta tcatttcaga ggaattattt gattaaggtt ttacttaagg
     9481 cgtaacaaat gatatttatc ggcaaactga acttctcctt taggatgttt tcactccccg
     9541 aaatggggat aaaaggcaaa taaaataact aaaggattta ttcaatgaaa agaaaagtac
     9601 tggcactttt aatccctgcc ctgctcgccg caggtgctgc acatgcagcc gaagtttata
     9661 ataaagacgg caacaaatta gatctgtatg gcaaagttga tggcctgcat tatttttctg
     9721 ataattcagc gaaagatggc gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc
     9781 aaattaacga tcaacttact ggctacggtc aatgggaata caatattcag gcaaacaaca
     9841 ctgaatcttc aaaaaaccag tcatggaccc gtctggcatt tgccggactg aaatttgcag
     9901 attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc gaaggctgga
     9961 ccgatatgct gcctaaattt ggcggtgact cttataccaa tgcagacaac tttatgactg
    10021 gtcgagccaa tggcgtcgcg acttatcgta atactgattt cttcggtctg gtaaatggtc
    10081 tgaacttcgc ggtgcagtat caaggtaaca acgaaggagc cagtaatggt caggaaggca
    10141 ccaataacga acgcgatgtt cgtcatgaaa acggtgacgg ctggggtctt tccacaacat
    10201 atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac cgcaccaatg
    10261 accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact gctgggctaa
    10321 aatacgatgc taacaatatt tacctagcaa ccatgtattc agaaacgcgt aatatgaccc
    10381 cgtttggcga cagcgattat gcagttgcga acaaaaccca gaactttgaa gttactagta
    10441 ccagtttgat tttggtctgc gtccggcagt ctctttcctg atgtctaaag gccgtgacct
    10501 tcacgctgct ggtggtgcag acaacccggc aggtgttgat gataaagatc tggttaaata
    10561 cgccgatgtt ggcgcgactt actatttcaa taaaaacatg tccacctatg ttgactataa
    10621 aatcaacctg ttggatgaag atgacagttt ctacgctgcc aacggcatct ctaccgatga
    10681 tattgtcgct ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg ttatggcggg
    10741 ctttttctgc ttattcttcc tcctttgatc taaattaaaa atgtgaactc cgtcattaca
    10801 caaaaagtgt catctggcgt tacactttat gcggatacta aaacaggagg ttttatgaac
    10861 agaacgattc ttgtccctat cgatatttca gattcagaat taactcaacg cgtgattagc
    10921 cacgttgagg cagaggcaaa gattgatgat gcagaggttc atttcctgac ggtaatacct
    10981 tcactgccct actatgcctc tctgggttta gcgtattccg cagaattacc ggcaatggat
    11041 gacctgaaag cggaagccaa atcgcaactg gaagagatca ttaaaaaatt taaactgcca
    11101 accgacagag tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat tctggaattg
    11161 gcgaagaaga tccccgctca tatgatcatc attgcttccc atcgaccgga tatcaccact
    11221 tatctgctcg gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt
    11281 gtgcgctgac actaacgccc gcacattgct gcgggctttt tgattcattt cgcaaatgtg
    11341 ctgacatttt cccctctaat ccgtaccata cacgccacag tttttatatc agatttctta
    11401 tgctgagcgt tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt
    11461 ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga acaaattaca
    11521 aaaacgtctg cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg
    11581 cgatcgcatc atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct
    11641 gcgcaatttg cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca
    11701 aaagcaaccg ggcttcccgg aacacgttct gcccgagtat cttgaaaagc tgggcgttga
    11761 gtacaagatt gttgaagaga acacttacgg tatcgtgaaa gagaagattc cagagggcaa
    11821 aaccacttgc tcactgtgtt ctcggctacg tcgcggtatc ctttatcgca ccgcaacgga
    11881 actggggacg acgaagatcg cgttgggtca ccatcgtgac gatatcctgc aaacgttgtt
    11941 cttaaatatg ttctacggcg gcaagatgaa aggtatgcct ccgaaactga tgagcgatga
    12001 tggcaaacat atcgttattc gtccactggc ctactgccgc gagaaagata ttcagcgatt
    12061 tgccgatgca aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct
    12121 gcaacgtcag gtgattgctg acatgttgcg tgactgggat aaacgttatc cagggcgtat
    12181 cgagacgatg ttcagcgcga tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct
    12241 gttcgatttc aaaggcatta cccacggttc tgaagtggtt aacgggggtg atctggcgtt
    12301 tgatcgcgaa gagatcccac tacaaccggc gggctggcag ccagaagaag atgaaaatca
    12361 gttggatgag ttacggctga atgtggttga agtgaaataa ccaggatagc gcccgatgcg
    12421 caagcgtatc gggctactct tatggaggcc ggataagacg cggccagcgt cgcatccggc
    12481 aatcccgaat aagatgttta ctcttgcacc cggcaattca acatttcatt attttaataa
    12541 ccgcacccgg cacgtttttc ctttaatctt cccgccctgt aactgtttcc atgctttatg
    12601 agcaacagcc tgacggaccg cgacatagac atgcgccgga tgcacggcga ttttgccaat
    12661 atctgcgcca tcaagcccga tatctcctgt cagtgcacct aatacatcac ccgggcgcat
    12721 tttggctttt ttcccgccat cgatacacaa cgttgccatt tctgcttcca gcgtcacaat
    12781 ggaactatta gctggcggcg tttgccagtt aagttttatc tgcaacatgt cagaaatgat
    12841 attggcccgc tgtgcttctt ccggagcaca gaaactgatc gccagaccgc tatttcctgc
    12901 acgagctgta cgaccgatgc gatgtacatg aacttcaggg tcccacgcca gctcaaagtt
    12961 caccaccagc tcaagcgatt taatatccag accacgcgca gcaacatcag tcgcaaccag
    13021 tacacgggcg ctaccgttag caaaacgtac cagggtctga tcgcgttgct ccagatcgcc
    13081 gtgtaacgac aatgcacttt gctctacttc attcagcgcg tcgcagacag cctggcaatc
    13141 ttttttggta ttgcaaaaca ccacacaaga ggatggctga tgcaagctta ataaccgttg
    13201 caacagagga attttgcctt tactggatgt ctcataaaat tgttgttcaa tgggtggcaa
    13261 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattgc
    13321 ggcgatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
    13381 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
    13441 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
    13501 acgacccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac gctgcatacc
    13561 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
    13621 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagccag
    13681 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
    13741 gccgctgccg gttttcgcct gcacgcgaac atcttttcct gcaaggatcg ccggaagcgc
    13801 ggcggcctgc accggcgtca tggttaaata acccaactca ttaaggttcg tgagttgggc
    13861 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
    13921 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
    13981 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
    14041 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
    14101 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
    14161 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
    14221 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
    14281 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
    14341 tttgtataca gacagccgaa aaaattgctt ttgttacaac catttactac ggtgcaacca
    14401 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
    14461 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
    14521 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
    14581 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
    14641 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
    14701 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
    14761 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
    14821 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
    14881 actggaatga tcggtcaacg catcgcacac atccaccagc catcccccgc aatcggtcgg
    14941 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
    15001 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
    15061 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgaaacggct
    15121 gactcggggc cgtgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
    15181 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
    15241 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
    15301 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt
    15361 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
    15421 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
    15481 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
    15541 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
    15601 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
    15661 ggattgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
    15721 tgcaagacct cacgtttagt cagctacgaa ccggacccta ttccgtctcc tcacaaaaag
    15781 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
    15841 ttcagcgtaa agaagaggaa acagcattaa gctgtcggct tgttttgcga gagctgacag
    15901 aaacagaacc ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg ggtttaaaag
    15961 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
    16021 ctaactctgc accaatgttg ttgattgacc cgtcacgaga tggacaaatc gtcgatgcta
    16081 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
    16141 aggaaataaa tatgcttggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
    16201 ccggcggtca taaccctttg aattttgttc ataaactggc ggatggttct actcgtcatg
    16261 tgcagaccta tgccggaccg attgaaattt atggcgacaa gctcatgtta tgtattgtgc
    16321 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgct caccatgacg
    16381 cgatgaccgg attactgaat cggcgacagt tttatcacat tacggaacca ggccaaatgc
    16441 agcatctcgc catcgctcag gattacagct tgttgctcat cgacaccgat cgttttaaac
    16501 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgcc
    16561 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
    16621 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
    16681 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
    16741 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
    16801 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
    16861 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
    16921 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgcg gtgcaatatg
    16981 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
    17041 gtgatttatc ttcccgccct ttaccatgaa taatcagcac attacgcaaa ccatccgcca
    17101 gggcttgttg aatgaaactg aacaccattt tgcggcactc ttccaccggc tggcgcaaaa
    17161 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgct
    17221 gcaacccttc ctgccgaaac tccagcggct gacttagcgg gatgatgtcg agaaatccgg
    17281 tagtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
    17341 ttggatgcca gtggacatct gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
    17401 gaaacagcga tttgtcgtca aggttcatgt aacctcctat gcaccttcac acgtcgtgat
    17461 tcagacgggt taattgttca tacttactgc atcgttccct tcacagattg aaacaatact
    17521 ctacttcata cctgtgagta tctgcgcagg cggggcattt tatactagcc tgttagcagt
    17581 atcgaacagt ttagcccgag tttaattcag cacaggctcc gatcaggatg atttattcat
    17641 agcaggaacc aacaaaaatg cgataaataa cagaccactg attagcagaa aggtaccgaa
    17701 aatgccatag tttgcgcctc cctcagcatc aattgactca ataatttggt tgaagccagt
    17761 atttccgatg tctggtcact tctactgcga ttaaaaaata aagcacttct ggaaatgaag
    17821 tcattcaccg tatggggaac agaaaccatt ttttcaccca ttctcgaaaa atgaaaatta
    17881 gttcgattat tgagaataga gccacacagg gcaataccaa tcgcgccccc aagattacgt
    17941 gttaaattaa atacaccaga ggctaacttc agccgttctt ttggaattcc ccccagcgtt
    18001 aatgttacga tgggggccat cgcaaactgc tgcaaaatcc ctctaattgc ctgtggaaac
    18061 agtaattcct gccatcccca ttcgtgggtt ataggggtaa aaagatacat tgaaaacaca
    18121 aatccgccca gccccgccat caacagccat cgcagattta tttttttact taaccagaag
    18181 taaaagggaa cagaaaataa ctggaaaata cctgttgtgc aaacggcaaa acctatctct
    18241 tcggcattta atccgcgaat ctgccccaaa aacacaggta ttaaatatac agtagagaat
    18301 atcccaaccc ctccagaaaa agaaaaaaaa catcccagag tgaaattttt atccttaaaa
    18361 gcatgaagat ccattatcgg attggatatt gtaagtgtac gcgcagcaaa taaaataaaa
    18421 ctcaccaatg ccagaactga tgtcagcaaa atagtattat catccagcca tccccagcgc
    18481 gctccttctt caagggtata ttccagacat cccagagtca tggccaacag gataatactc
    18541 ggataatcag caactttcag taaagagaga tcaggtttat caaaattaac cagaaaagga
    18601 atactcaaaa ctaaatatat gccaggtaat atattgatat aaaatagcca tcgccagtcg
    18661 aggttatcag tgagccagcc ccccagagtt ggccctagcg tcggtgacag tgaggccagc
    18721 gcactgacta ccgcagcagc caaccccaac tctttaccct ggtaataaat aaacgccgtc
    18781 gtgaaaacta aagggatcat ggatgcgcca gcagctccct gtaaagcacg aaacaaaatc
    18841 atgatctgaa tattccatgc aagtccacac gcgatactca ttaaagtaaa tattcccgcg
    18901 gatagagtaa aaagccaacg agtggaaaat acccgtgaca gccacccaga gagcggaata
    18961 acaataatct cagcaattaa atagctggtc tgaagccagg ccatttcgtc tttacctgcg
    19021 gacagaccac cacctatttc atttagggat gacgatacaa tttgaatatc aagctgagca
    19081 ataaataatc ccatacacat actggcgaaa gcaatatgtt tacgcataga catcatctct
    19141 catcaacact gacggtgacg gacagtcccg ggcgaagaag ttgctttaat tcctttgcgt
    19201 cgtcaaatgc aatccgtacc gggacacgct gaacaatttt ggtgaaattg ccagtggcat
    19261 tttctatcgg aatcaagctg aaactggctc ctgtcgctgg ggaaaggctt tcaatgtgac
    19321 cgtgaaacac ttcccctttg agaatatcag cacggatctc agccttcatt cctggcttca
    19381 ttcccgctat ttggttttct ttataatttg catcaaccca caactcactg actggtacta
    19441 gtgaaactaa cgatgtgccg ccttccaccc aactgcctgt atgggcgctg cggtttgcga
    19501 caatgccatc aatcggcgca
//
LOCUS       DAJTXY010000074        19167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTXY010000074 DAJTXY010000000
VERSION     DAJTXY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.074"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..1650)
                     /gene="glmS"
                     /locus_tag="OO566_002639"
     CDS             complement(<1..1650)
                     /gene="glmS"
                     /locus_tag="OO566_002639"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6605870.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQ"
     gene            complement(1812..3182)
                     /gene="glmU"
                     /locus_tag="OO566_002640"
     CDS             complement(1812..3182)
                     /gene="glmU"
                     /locus_tag="OO566_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6605871.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGAGMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3534..3953)
                     /gene="atpC"
                     /locus_tag="OO566_002641"
     CDS             complement(3534..3953)
                     /gene="atpC"
                     /locus_tag="OO566_002641"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6605872.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(3974..5356)
                     /gene="atpD"
                     /locus_tag="OO566_002642"
     CDS             complement(3974..5356)
                     /gene="atpD"
                     /locus_tag="OO566_002642"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6605873.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5383..6246)
                     /gene="atpG"
                     /locus_tag="OO566_002643"
     CDS             complement(5383..6246)
                     /gene="atpG"
                     /locus_tag="OO566_002643"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6605874.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6297..7838)
                     /gene="atpA"
                     /locus_tag="OO566_002644"
     CDS             complement(6297..7838)
                     /gene="atpA"
                     /locus_tag="OO566_002644"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6605875.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(7851..8384)
                     /gene="atpH"
                     /locus_tag="OO566_002645"
     CDS             complement(7851..8384)
                     /gene="atpH"
                     /locus_tag="OO566_002645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6605876.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8399..8869)
                     /gene="atpF"
                     /locus_tag="OO566_002646"
     CDS             complement(8399..8869)
                     /gene="atpF"
                     /locus_tag="OO566_002646"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6605877.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(8931..9170)
                     /gene="atpE"
                     /locus_tag="OO566_002647"
     CDS             complement(8931..9170)
                     /gene="atpE"
                     /locus_tag="OO566_002647"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6605878.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9217..10032)
                     /gene="atpB"
                     /locus_tag="OO566_002648"
     CDS             complement(9217..10032)
                     /gene="atpB"
                     /locus_tag="OO566_002648"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6605879.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10041..10421)
                     /gene="atpI"
                     /locus_tag="OO566_002649"
     CDS             complement(10041..10421)
                     /gene="atpI"
                     /locus_tag="OO566_002649"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6605880.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11038..11661)
                     /gene="rsmG"
                     /locus_tag="OO566_002650"
     CDS             complement(11038..11661)
                     /gene="rsmG"
                     /locus_tag="OO566_002650"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6605881.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11725..13614)
                     /gene="mnmG"
                     /locus_tag="OO566_002651"
     CDS             complement(11725..13614)
                     /gene="mnmG"
                     /locus_tag="OO566_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6605882.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(13993..14436)
                     /gene="mioC"
                     /locus_tag="OO566_002652"
     CDS             complement(13993..14436)
                     /gene="mioC"
                     /locus_tag="OO566_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6605883.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14526..14984)
                     /gene="asnC"
                     /locus_tag="OO566_002653"
     CDS             complement(14526..14984)
                     /gene="asnC"
                     /locus_tag="OO566_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6605884.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15136..16128
                     /gene="asnA"
                     /locus_tag="OO566_002654"
     CDS             15136..16128
                     /gene="asnA"
                     /locus_tag="OO566_002654"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6605885.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16133..17584)
                     /gene="viaA"
                     /locus_tag="OO566_002655"
                     /pseudo
     CDS             complement(16133..17584)
                     /gene="viaA"
                     /locus_tag="OO566_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17578..19074)
                     /gene="ravA"
                     /locus_tag="OO566_002656"
     CDS             complement(17578..19074)
                     /gene="ravA"
                     /locus_tag="OO566_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6605886.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4581 a         5066 c         4809 g         4711 t
ORIGIN      
        1 ctgatcggcg aagacataca attgaccgcc acgcgcgcga acttcttcaa tgttggattt
       61 cagtttttcc aacagttcgt tgttcggtgc aacgacgata accggcatat ccgcgtcaat
      121 cagcgccagc gggccgtgtt tcagctcgcc tgcagcgtag gcttcagcgt gaatgtaaga
      181 gatctctttc agcttcaatg cgccttccag cgcgattggg tactgatcgc cacggcccag
      241 gaacagcgcg tgatgtttgt cagagaaatc ttctgccaga gcttcaatgc gtttatcctg
      301 agacagcatc tgctcaatac ggctcggcag cgcctgcaga ccatgcacaa tgtcatgttc
      361 aatggaggca tccagacctt tcaggcgaga cagcttcgcc accagcatca acagcacagt
      421 taactgagtg gtgaatgctt tagtggacgc cacgccgatt tccgtacccg cgttggtcat
      481 cagcgccaga tcggattcgc gcaccagaga agaacccgga acgttacaga ttgccagcga
      541 accaaggtaa cccagctctt tcgacagacg caggcccgcc agggtatccg cggtttcgcc
      601 agactgtgac aaggtgatca tcaggctgtt acgacgcacg gcagatttac gatagcggaa
      661 ttcagaggcg atttcgacgt cgcacggaat acctgccagc gattcaaacc agtagcggga
      721 aaccataccg gagttataag aagtaccaca ggcgaggatc tgaatatgct caaccttcga
      781 cagcagttcg tcggcgttcg gtcccagctc gcttaaatca acctgaccgt ggctgatgcg
      841 cccggtaagg gtgtttttta tcgcgttcgg ctgttcgtag atctctttct gcatgtagtg
      901 acggtaaata cctttatcgc ccgcgtcata ttgcagattg gattcgatat cctgacgttt
      961 tacttccgcg ccagttttat cgaagatgtt taccgaacgg cgagtgattt ccgcaatatc
     1021 gccctcttca aggaagataa agcgacgggt caccggcaac agcgccagct ggtcagaagc
     1081 gataaagttt tcgcccatac ccaggccaat caccagcgga ctaccagaac gtgccgccag
     1141 cagggtatcc gggtgacggg agtccatgat cactgtaccg tacgcaccgc gcagctgcgg
     1201 gatagcacgc agaacagcct cacgcagagt cccgccttgt ttcagctccc agttcaccag
     1261 atgggcaatc acttcggtgt cggtttcaga aacgaaggta tagccacgcg cttttaactc
     1321 ttcacgcagc ggttcatggt tttcgatgat gccattatgc accaccacaa tgtgttcaga
     1381 aacatgcgga tgcgcgttag cttctgaagg ttcaccgtgg gtcgcccagc gagtgtgagc
     1441 aataccggtg ccgccatgca gaggatgttc ttccgctgcc tgagccagca tctggacttt
     1501 accgaggcga cgcaggcgag tcatatgacc ttccgcatca acaacggcca gaccggcaga
     1561 gtcatatccg cggtattcca gacgacgtaa accttcaaga aggatttctg ctacatcacg
     1621 ttgcgcgatc gcgccaacaa ttccacacat agtttttgat tccgatttat atcgttgtcg
     1681 gtcaacctgt atgcccgttt tttcgggcgc cccgagcctt gtagagagtg gggttatttt
     1741 tatagttact gcttgtggga gggagattat gttatctcct catcccagat gaccggatta
     1801 cccggccaga attacttttt ctttaccgga cgacgccagc cttctttctg agtctgcggc
     1861 acacggctga tagctaatgc attttcgccg acattacgcg tcacagttgt acccgcagca
     1921 atggtcgcgc ctttgcctac tgttaccggg gccaccagct gagtgtcgga accgacaaac
     1981 acatcgtcgc cgataatggt cttaaattta ttcgcaccat cgtagttgca ggtaatggtt
     2041 cccgcgccga tgttaacgtt atcgccaatt tccgcatcgc ctaggtaagt cagatgcccg
     2101 gctttcgtgc ctttacccag acgcgctttt ttcatctcaa cgaagttacc aacgtgtgca
     2161 ccttccagca actcagcacc aggacgcaga cgggcaaacg ggccaatagt acaggccgct
     2221 gccagattcg cgtcttccac aacggtatac ggactgattt cgcaatcatc gccaatcacg
     2281 ctgtttttaa tcacgcaacc ggtgccgatt ttcacgcgat gaccgagagt cacgttgccc
     2341 tcgatgataa cgttagtatc aatttcaaca tcgcgcccgt gagtaagcgt accgcgcaga
     2401 tcaaaacgcg ccggatcgcg cagcataacg cctgctaaca gcagtttttc agcctgttcg
     2461 gactggtaaa cgcgctccag acgggagagt tgcaggcggt tattcacacc ttctacttcg
     2521 cttaaacgtt gcggatgaac ggcgacgatt tcacgccctt cctgatacgc cagcgcaata
     2581 atgtcggtga tgtagtattc gccctgagca ttattgttgg tcagcttcgc cagccagcgt
     2641 ttcatacctg cgccgttggc aatcagaatg ccggtgttga tctcctgaat ctgacgctgc
     2701 tcgtcggtgg catctttgtg ctcaacaatg ccggtaactt tgccattttc acgggtgata
     2761 cgtccataac cggtcggatc atccagtttc accgtcagca gaccaatgcc accctggggt
     2821 ttagcatcac gcaggcgctg gagtgtttcg acagagatca gcggcacgtc gccgtagagc
     2881 attaaaatgt cttcatcatc ggcaaagaaa ggtgcggcct gctgcattgc atgacccgta
     2941 cccagctgct ctgcctgaag cacccagtta aggttgtcgt ctttcagcgc ctgtttaagc
     3001 agatcgccgc cgtgaccgta caccaggtga acgtgcgctg cgcctaattc attcgcagca
     3061 tcaatgacat gctgaaccat cgctttcccg gcaagggtat gcagcacttt cggaagatcg
     3121 gaatacatgc gcgtgccttt gcctgcggca aggatcacta cgctcatagc attattcaac
     3181 atacgcgtcc tgactgtaat ttgagaacga atttaaaccg cttcaccttg aaaaaactac
     3241 atttttttca tcgtgaaatg gacagaggat aaattgttca ataacggatt atccccgctg
     3301 acgaaagtgc cattttcgac catcgtcacg ccttttgtct gcagaaaatt aaggcagtac
     3361 cttcatttaa gcgtcaggtg gatgtttttg ctcttatttc gatcaatgaa taaacgggaa
     3421 aacagggggg ttttatatca gcaggatcta tgtgaacgct cttcaggacg ggtcaacgcg
     3481 taaaaaaaag ccagcctgtt tccagactgg cttttgtgct tttcaagccg gtgttacatc
     3541 gcttttttgg tcaactcgat aacgcgcagc tgcgcgatcg ctttggccag ttccgcagac
     3601 gcctgagcgt aatctacgtc gccgtgagag ctgctaatgt gctcttcagc cttacgtttc
     3661 gcttccatgg ctcgcgcttc gtcgagatcc tggccgcgaa ttgcagtgtc ggccagaacg
     3721 gtcacgttgc caggctgcac ttcaagaatg ccgccagaca gatagataaa ctcttcgtga
     3781 ccgtgctgtt tcacgatgcg aatcatacca ggcttaatgg cggtgagcag cggtgcgtgg
     3841 ccagggtaga tccccagttc accttcgcta cccgttacct ggattttctc gaccagacca
     3901 gagaacattt gttgctctgc gctgacgacg tccaggtggt aagtcattgc catatcaccc
     3961 tccgattaag gcgttaaagt tttttggctt tttccacagc ttcttcgatg gaaccgacca
     4021 tgtagaacgc ctgctccggc aggtgatcgt attcgccttc catgatgcct ttaaagccac
     4081 ggatggtgtc tttcagggag acgtatttac ccggagaacc ggtgaatact tctgccacga
     4141 agaacggctg ggacaggaag cgctggatct tacgagcacg cgctaccacc agtttgtctt
     4201 cttcagacag ttcatccata cccaggatgg cgatgatgtc tttcagttcc tgataacgtt
     4261 gcaggatgga ctgaacgcca cgcgcggtgt cgtagtgttc ctgaccaacc accagcgggt
     4321 ccagctgacg gctggtggag tccagcgggt caacggccgg gtagataccc agagacgcga
     4381 tctgacggct cagtaccacg gttgcgtcaa ggtgcgcaaa ggtggttgcc ggagacgggt
     4441 cagtcaagtc atccgcaggt acgtataccg cctgtacgga ggtgatagaa ccagttttgg
     4501 tggaggtgat acgttcctgc agaacgccca tctcttccgc cagggtcggc tgataaccta
     4561 ccgctgaagg catacggccc agcagtgcgg atacttccgt accggccagg gtgtaacgat
     4621 agatgttgtc aacgaacagc agaacgtcac gaccttcgtc acggaatttc tcagccatgg
     4681 tcagaccggt cagagcaacg cgcagacggt ttcccggcgg ctcgttcatc tggccataca
     4741 ccagggatac tttgtcgata acgttggagt cggtcatttc gtggtagaag tcgttaccct
     4801 cacgagtacg ttcacctacg cccgcaaaca cagagtaacc ggagtgctcg atcgcgatgt
     4861 tacgaatgag ctccatcatg tttacggttt tacctacacc cgcaccaccg aacagaccaa
     4921 ctttaccgcc cttagcgaac ggacacatca ggtcgataac tttgataccg gtttccagca
     4981 gttcctgaga gtttgacagc tcttcgtagg aaggtgctgc gcggtgaatc gcccaacgct
     5041 cttcttcacc gatctcgcct ttcatgtcga ccggttcacc cagtacgttc atgatacggc
     5101 ccagagtcgc tttacctacc gggacttcga tcgggtgttc gaggtctttt acatccagac
     5161 cgcgacgcag accgtcggag gaacccattg cgatggtacg cacgataccg ccgccgagct
     5221 gctgctgaac ttccagcacc agacgctcat taccattttg cacctcaaga gcatcgtaca
     5281 cgcgcggtac ggcatcctga gggaattcga cgtcaactac ggcgccgatt acctggacaa
     5341 tctttccagt agccatctta aatcctctac gaaataacct gtttaaaccg cggcggcccc
     5401 cgagacgatc tcggtgagtt cctgagtaat gctggcctga cgagctttgt tgtataccaa
     5461 ctgcagctct ttaatcaggc tgccgccatt gtcggtcgcg gctttcatcg ccaccatacg
     5521 ggcggcctgc tcgctggcca ggttttcaac cacgccctga taaacctgag attcgacata
     5581 acgacgcagc agggtatcca gcaacgcctt cggatcgggt tcgtacaggt aatcccagga
     5641 tttatgtttc agatcatcat catctgatgc cggtaacggc agcagctggc tgatggtcgg
     5701 aacctgagac atggtgttaa taaatttgtt gctgacaatg taaagcttgt ccagacggcc
     5761 ttcgtcgtag gcctgcaaca tcacttttac cggaccgatc agttcggaca gggaagggtt
     5821 atcccccatg ccggtgacct gggcaacaac attgccgccc acggagttga agaacgacac
     5881 gcctttcgag ccgatcattg cgaggtcgca ttgaacgcct ttgtcggtcc aggtcttcat
     5941 ttccgccagc agttttttga acaggttaat gttcaaacca ccgcacaaac cacggtcggt
     6001 cgacaccacc aggtagccca cgcgtttaac gtcgcggtct tccaggtaag ggtgcttata
     6061 ttccagatta ccgtgtgcaa ggtgaccaat cactttgcgc atggtttctg cataaggacg
     6121 gctggccgcc atgcgatcct gcgatttacg cattttggaa gcggcgacca tctccatcgc
     6181 tttagtgatc ttttgcgtgt tctggacgct tgcgatctta ctacgtatct cttttgcgcc
     6241 ggccatgagc ttctcctcaa tgccttgcgg cctgccctaa ggcaagccgc cagacgttac
     6301 caggattggg ttgctttgaa ggaatcgagg atgcctttca gcttgccttc gatttcgtcg
     6361 ttgtagccac cggtctggtt gatctcttgc atcaacggag cgtgatcacg gtcgacgtaa
     6421 gccagcagag cggcttcgaa gctgccaatt ttcgacagtt caacatccgc caggtaacca
     6481 cgttctgctg cgaacagaac cagagactgc tgcgcaacgg acatcggcgc atactgtttc
     6541 tgtttcagca gttcggtcac tttctgaccg tggtcaagct gcttacgtgt tgcatcgtca
     6601 aggtcggatg caaactgaga gaacgctgcc agttcacgat actgtgccag agcggtacgg
     6661 ataccaccgg acagtttttt catgatcttg gtctgtgctg caccaccaac acgggatacg
     6721 gaaatacccg ggttaaccgc aggacgaata ccggcgttga acaggttggt ttccaggaag
     6781 atctgaccat cggtaatgga gattacgttg gtcggaacga acgcagaaac gtcacccgcc
     6841 tgagtttcga taatcggcag tgcggtcaga gaaccggttt tccctttcac ttcacctttg
     6901 gtgaaggctt caacgtattc ggcgttaaca cgtgcagcac gctccagcag acgagagtgg
     6961 aggtagaaaa cgtcgcccgg gaatgcttca cgtcctggcg gacgacggag cagcagggag
     7021 atctgacggt aagcaacagc ctgtttagac aggtcatcgt aaatgatcag cgcatcttca
     7081 ccgcggtcac ggaagtattc gcccattgcg caaccggcat acggtgccag gtattgcagt
     7141 gcagcggatt cagacgcggt tgctaccaca acgatggtgt tagccagtgc gccgtgctct
     7201 tccagtttac gtaccacgtt agaaatggtg gacgctttct ggccgatagc gacatagata
     7261 catttgatac cggaatcgcg ctggttgatg atggcatcga tagccagtgc ggttttacct
     7321 gtctgacggt caccgatgat caattcacgc tgaccacgac cgattgggat catggagtca
     7381 acggctttat aaccggtctg taccggctga tctacggact gacgttcgat aacgcccgga
     7441 gcgattgctt ctacagcaga gaagccgtcg tgatccagcg gacctttacc gtcgattggt
     7501 gcacccagag tgttaaccac acggcccagc aggccacggc caaccggaac ttccaggata
     7561 cggccagtac acttaacttt catgccttcg gcaaggtcag cgtacggacc cataacaacc
     7621 gcacctacag agtcgcgctc aaggttcagt gcgatagcgt aacggttacc cggcagggag
     7681 atcatttcac cctgcataca atcggccagg ccgtgaatgc ggataacacc gtcacttaca
     7741 gaaacaatag taccttcgtt gtgagcttca ctcacaacat tgaactgagc aatgcgctgc
     7801 ttgatcagtt cgctgatttc ggtggaattc agttgcatgc tccagtcccc ttaagactgc
     7861 aagacgtctg caaggcgctc aagacgaccg cgtacgctgc catcaatgac catatcaccc
     7921 gctcggatga taacgcctgc cattacagac ttatcgattt tgcaattcag cttaactttg
     7981 cgtgacagac gtttttccat cgcagcagaa attttcgcga gctgttgttc actcagtgcg
     8041 gcagcggaaa tgacgtctac ctcagcggta gcctcactca cggcacgaag gtgaataaac
     8101 tgctccagaa catccgggag cgcgttaaga cgaccatttt cagccattac ccgaatcagg
     8161 ttctgaccgt tttcgtccag ttgctcacca caaactgcga taaacgactc ggcgagcgtt
     8221 tctggcgcaa gcgcgccaga gagaagctct gccatttgtt cgtttttggt tacctcggcg
     8281 gcaaacgcca gcatgtcctg ccagcgttct acactttggt gttcgacggc aaagtcaaaa
     8341 gctgctttgg cgtaggggcg agctaccgta ataaattcag acatcagccc ctccctcctt
     8401 acagttcagc gacaagttta tccacgatgt cgctgttagc agcttcatcc acggaacgtt
     8461 cgatgatctt ctcggcgcca gcaacagcca ggatagcaac ttgcttacgc agctcttcac
     8521 gggcacgttt acgctcggct tcaatttcag cctgcgcctg ggccacgatt ttagtacgtt
     8581 cctgttctgc ctcagctttc gcttcgtcca gaatctgcga gcggagttta ttcgcctgct
     8641 cgatgattac ctgggcttcc gctttcgctt ttttcagctg gtcggtcgcg ctggcctttg
     8701 caaggtcaag gtccttatgt gctcgttctg cggaagcaag gccgtcagca atttcttttt
     8761 gacgtttttc gatggctgcc attaatggcg gccatacgta cttcatgcag aacagaacga
     8821 acaggacaaa cgcgatggcc tggccgagga ttgttgcgtt aagattcaca gcacaatgcc
     8881 tctatttagt taacgttctg atattgctct ttaaataaaa gcaacgctta ctacgcgaca
     8941 gcgaacatca cgtacagacc cagacctaca gcgatcatcg ggatagcatc caccagaccc
     9001 ataacgataa agaactgagt acgcagcaga ggaatcagat caggttgacg cgctgcgcct
     9061 tccaggaatt tacccccgag gatgccgata ccgatcgcag caccgattgc cgccagaccc
     9121 atcatcacag cggcagccat gtacagcaga tccatattca ggttttccat gacagtctcc
     9181 agtttgtttc agttaaaacg tagtagtgtt ggtaaattaa tgttcttcag acgccatcga
     9241 cagatagacg atcgtcagaa ccatgaagat gaaggcttgc agcgtaatga tcaggatgtg
     9301 gaaaatggcc cacggcacat tcaggatcca ctgtgaccac cacggcaaca gaccagcaat
     9361 cagaatgaaa atcagctcac cggcatacat gttaccgaac agtcgcaaac cgagtgaaac
     9421 tggtttggac agcaggctta ccccttcaag gattaagttg acaggaatga acgcccagtg
     9481 attgaacggc tgcagcgtca actctttcgt gaagccgccg atgcctttca ttttgatgtt
     9541 gtagaacaga atcaggataa atacgcccag tgccatagac agcgttacgt tcacgtccgc
     9601 agacggaacc acacgcagtg caggcagacc cagtacatgt tcagcaatgt acggcagcaa
     9661 gtcgataggc agtaaatcca tcagattcat caggaatacc cagacgaaga tcgtcagggc
     9721 cagcggagca atcagcttgc ttttgccatg gtacatgtct ttcacgctac cattaacaaa
     9781 gccgatcacc agctcaatcg cggtctgaaa cttacctggc acaccgctgg tcgccttttt
     9841 ggctacgcta cggaataaaa ccaagaacaa cagacccagc accaccgaga agaacatgga
     9901 gtcaatattg attgtccaga aggtggctgg ggggttttgt ggatccacca gcgagaatgt
     9961 acgcaggtcc agctgaaggt tattcaggtg gtgtcctatg taatcctgcg gcgtcatatt
    10021 ttctgaagcc atgatgcctt ttaccctttg ttgttaatta cagccggtgc cagtatctga
    10081 accaccagca ccaaaaccca cgtaacgatc agcggcaaga ataccgcctt taaaaccgcc
    10141 aacgccacca ccagtaacac caacatcgcc agaactttga aagcttcgcc aaatgcgaat
    10201 gtccaagcca cccggccttt cgctggtgta tgcgcctggt gacgccaggc aaatatcata
    10261 aacaaaacgt taggcagaaa gactgccagg cccccgctta ttgcagagac gccccagaag
    10321 gggtctttga ggctgaacag caatccactt gctatcacca ccagtaactg aacgagcaga
    10381 agcttccgag caacgtttcg actcacgagc gacacagaca tcacgttttt cactcctgct
    10441 cccttcgagg tatgccgcgt gtcgtataaa actttcttta aggcttagag tcaagcatca
    10501 aaaagcggtc aaattatacg gtgcgccccc gtgatttcaa acaataagta gccaaaaggt
    10561 gaataaatgt ttaaatattt ttccagtgca tacaattgcg acttttccgc tacccctgtt
    10621 cgatcatgaa aaactgtaaa taacgcgtaa acactggtga taaagcgtgc ttcagatcac
    10681 atattgcgca tgttcgcgca cagcatattt atttacttgg caaatgatgc ctttgcaagt
    10741 ttatgatatt tcagtctaaa aacagatact gttttaataa atgacattta cacaacaaaa
    10801 accacccatt gacattttta ataatgtttt aacagccaat gatggttctt agcgccgatt
    10861 tttagcagac tgatattttc actaatgact tattttctgc ttaccaaaaa aagccacgtt
    10921 atcttgttga tgcaaaagag tgaacgtggc gttaaatgta accagttata tcagtagaaa
    10981 acctggttgt tgttaacagt ctaaccggtc aattttttat gatttttttg ataaaaatta
    11041 aattttattt gctttaatca ccaccagatg acgttcgcca tccagggctg gaacctgaag
    11101 tttaaccact gattcgacct gatattcttc gggcaacaaa gcgatttcat cttccggcat
    11161 ttgccctttc agcgcgtaga aacggccttg ctcaccagga agatggtggc accagctcac
    11221 catatcgttc agagaggcaa aagcgcggct aattacgcca tcaaatggcg gctctgaagg
    11281 aaactcttct accctgctct gtactggttc aatattctcc agtttaagct catgttgcac
    11341 ctgacgaagg aaacgcacgc gtttaccaag gctatccaac agagtgaaat gggcttcagg
    11401 acgcacgata gagagtggaa tgcctggcag tcctggtccg gtgccgacat cgataaaccg
    11461 ttcaccttgc agatacggtg ccaccacaat gctatcgaga atatggcgta ccagcatctc
    11521 attaggatcg cggaccgaag tcaggttgta cgctttgttc catttatgca gcatattcac
    11581 gtaggcaata agctggtttt tctggtgatc agtaagcgaa atacctgcgt ctttcagcag
    11641 taaggagagt ttgttgagca cggtgattac ctgttcttga tgcgttgctt ggtaagcggg
    11701 tgcttaccag gcatttttaa tgcgttatgc gctacgacgc agcataccct gttttttcag
    11761 ccacaccagc agaatggaga tggccgcagg cgtgacgcca gaaatacgcg aagcctggcc
    11821 gatagaggcc ggtttgtgat cgttaagttt ggcgatcact tcgttagaaa gaccggatac
    11881 ctggcggtaa tccagtgtcg cgggtagcag ggtgttctcg ttacgcagct gcttttcgat
    11941 ctcatcttgc tggcgcgcga tataaccttc gtatttaacc tgaatctcaa cctgttccgc
    12001 cgcctgttcg tctgtcaacg caggggcaaa cggcgtcagc gtggttaatt tttcataagt
    12061 catttccgga cgacgcagca gatcttcacc actggcttca cgggaaagcg gcgcagtcag
    12121 gtgagcattc acttcggctg cagcttccgc cgacggggtt acccaggtcg atttcagacg
    12181 ctgacgctca cgctcgatat tctcaagttt ctcgttaaag cgcgcccaac gttcgtcatc
    12241 caccaggccc agttcacgac cgatttcagt caaacgcaga tccgcattat cttcgcgcag
    12301 catcagacga tattctgcgc gcgaagtaaa catacgatac ggttctttgg ttcctaaagt
    12361 gcacaggtca tcaaccagta cgccgagata cgcctgagaa cgtgccggag cccaaccttc
    12421 tttgtcagca gacagacggg cagcgttaag accggccagc aaaccttgcg cagcggcttc
    12481 ttcgtaaccg gtagtgccgt taatctgacc agcaaagaac agcccctgga taaacttgct
    12541 ctccagcgtc ggtttcaggt cgcgaggatc gaagaagtca tactcaatgg cataacccgg
    12601 acgcacgatc ttcgcgtttt ccatcccctg catagagcgg acgatttgca tctgcacatc
    12661 gaacggcagg ctggtggaga taccgttcgg ataaatttca ttagaggtca gtccttccgg
    12721 ttcaaggaag atctgatgct gatttctgtc tgcgaagcgc atgactttgt cttcgatcga
    12781 cgggcagtag cgtgggccga caccttcgat cacccctgca tacattgggc tacgatcgag
    12841 gttactgcgg atcacatcat gggttttctc gttggtatga gtgatataac acggcacctg
    12901 ctggggatgc agggacgcat tgcccataaa cgagaatacc ggcattgggt tatcgccatg
    12961 ttgttgcgcc agcacgctaa agtcgatggt tcgagcatca atacgcggcg gagtaccggt
    13021 tttcagacga ccaacgcgca gcggcagttc acgcaaacgg cgagagagcg gaatggatgg
    13081 cggatcgcca gcacggccac cgctgtaatt atccagaccg atatgaattt tgccgtcgag
    13141 gaacgtccca acggtgagca ccacagcttt ggcacggaac ttcagtccca tttgggtaac
    13201 agcaccgacc acgcgatcgt tttcgacaat aagatcttca accgcctgct ggaagatcat
    13261 caggttcggt tggttctcca gcgccgtacg taccgcctga cggtagcgca cacgatccgc
    13321 ctgagctcgg gtagcgcgaa ccgccggtcc tttgcttgcg tttagtatcc taaactggat
    13381 acccgcttga tcgatcgctt tcgccatcag accgccgagt gcatccactt cttttaccag
    13441 atgtcccttc ccaataccgc cgatcgccgg gttgcagctc atctgcccca gagtgtcgat
    13501 attgtgtgtc aaaagcagag tctgttgacc catacgcgcc gcagccatcg cggcctcggt
    13561 gcctgcatga cccccgccaa tgatgatgac gtcaaaagga tccggataaa acatggtgat
    13621 tgcctcgcat aacgcggtat gaaaatggat tgaagcccgg gccgtggatt ctactcaact
    13681 ttgtcggctt gagaaagacc tgggatcctg ggtattaaaa agaagatcta tttatttaga
    13741 gatctgttct attgtgatct cttattagga tcgcactgcc ctgtggataa caaggatccg
    13801 acttttaaga tcaacaacct ggaaaggatc attaactgtg aatgatcggt gatcctggac
    13861 cgtataagct gggatcagaa tgaggggtta tacacaactc aaaaactgaa caacggttgt
    13921 tctttggata actaccggtt gatccaagct tcctgacaga gttatccaca gtagatcgca
    13981 cgatctgtat acttatttga gtaaattaat ccacgatccc agccattctt ctgccggatc
    14041 ttccggaatg tcgtgatcaa gaatgttgat cttcagtgtt tcgcctgtct gttttgcacc
    14101 ggaatttttg agttctgcct cgagtttatc gatagcccca caaaaggtgt catattcacg
    14161 actgccaata ccgattgcgc caaagcggac cgcagaaaga tcgggcttct gttcctgcaa
    14221 tgcttcatag aaaggagaaa ggttgtccgg aatatctccg gcaccgtggg tggagctgat
    14281 aaccagccag atccccgagg cagataaatc ttccaacagc ggaccgtgca gcgtttcggt
    14341 ggtaaaaccc gcctcttcca gcttttcagc caggtgttct gctacatatt cggcaccgcc
    14401 gagggtgctg ccgctgataa gagtgatatc tgccataaac cgccaccttt attaagagtg
    14461 gcgtattgta cgctgtgaac gcgttgggat ctacctgtgg aaaagtatgg gattaaaaaa
    14521 gccgatcagg gcttgatggt acgcatgatc gggttttgca ggacgatcaa tgtctcggtg
    14581 gactgaattt catcaattgt ttggatcttg ttgataagta catgctggag agcgtcgatc
    14641 gaacggcaca taacttttat aaagatgctg tagtggccgg ttgtgtaata ggcttcagtg
    14701 acttcatcaa ggctttccag ctttgccagc gcggaagggt agtctttggc gctctttaat
    14761 ataatgccga taaagcagcc tacgtcataa ccgagctgct tcgggctgac atcaatacgc
    14821 gccccggtaa tgatccccgc ctgcttcatt ttctctactc gaacgtgaat cgtccccgga
    14881 ctgacgccaa attgtttcgc cagttcggcg taagcggtgc gcgcattgcc cattaatgct
    14941 tccaggatgc cacggtccag attgtcgatc agataatttt ccataggatt ttcttatgca
    15001 gattgatgat tcattctatt ttagccttct tttttaatga atcaaaagtg tgttaggctt
    15061 tttattgaat gattattgca tgtgtgtcgg tttttgttgc ttaatcataa gcaacaggac
    15121 gcaggagtat aaaaaatgaa aaccgcttac attgccaaac aacgccaaat tagcttcgtg
    15181 aaatctcact tttctcgcca actggaagaa cgtctggggc tgatcgaagt ccaggcgccg
    15241 attcttagcc gtgtggggga tggcacgcag gataacttgt cgggctgtga aaaagcggtg
    15301 caggtaaaag tgaaagctct gcctgatgcc cagttcgaag tggttcattc actggcgaag
    15361 tggaaacgtc agaccttagg gcaacacgac ttcagcgcgg gcgaagggct gtacacgcac
    15421 atgaaagccc ttcgccccga tgaagaccgt ctttctccgt tgcactcggt ctatgttgac
    15481 cagtgggact gggaacgcgt aatgggcgac ggtgagcgtc aattctcgac tctgaaaagc
    15541 acggtagagg cgatctgggc gggaattaaa gcaaccgaag ctgcggttaa cgaagagttt
    15601 ggcctggcac cgttcctgcc ggatcagatt cacttcgtac acagccagga gttactgtct
    15661 cgttatccgg atctcgatgc caaagggcgt gagcgggcga ttgcgaaaga tcttggcgcg
    15721 gtattcctcg tcgggattgg cggcaagttg agtgatggtc atcgtcacga cgtgcgcgca
    15781 ccggattatg atgactggag caccccgtca gagctgggcc atgcgggcct gaacggcgat
    15841 attctggtgt ggaacccggt actggaagat gcgtttgagc tttcttccat ggggatccgc
    15901 gtggatgccg acacgctgaa gcatcagctg gcgctgaccg gtgatgaaga tcgtctgcag
    15961 ctggaatggc atcaggcgct gctgcgcggt gaaatgccgc agaccatcgg cggcggtatc
    16021 ggccagtctc gtttgactat gctgctgctg caactgccgc atatcggcca ggttcagtgt
    16081 ggagtatggc cagttgctgt tcgcgagagc gtcccttctc tgctgtaata atttatcgcc
    16141 gccagcgtct gagcaggcgg cttcgcatcc cggtatcaaa gcgccagata tgatcgaaaa
    16201 tgcgcatgat gccgggtttg ccgtgtgccg acatcgccac ggcatgaaag cgatgctgat
    16261 gtacccgctg cagctctttc actttactcg tcacgtcgtc aggcaaccgc tgagcgataa
    16321 aatcagaaat caccaccgca tcggcatcaa accattccct gctttgcaag cgttccataa
    16381 tggcgcgaaa acaactggca agatcggtgc cgccacgaaa ctgctggctt aaaaaacgga
    16441 ttgcttgttc gatgccttgt gggcctgaaa gctcataacg gacgatctcg gtggaaaata
    16501 gcataatata acagcgccgg ttttcagcga gagcaatgcg catcaaagcc aggcagaacg
    16561 ctttcgcaca ctgttcatta aagccgccca ttgagccgga agtatccaca cagacaataa
    16621 acggcccgcg cggctgttca tcgtaatctt tatgtaccac cggacgttcg agcacttttt
    16681 cacgccacga ctcaccgtgc aggcgatagg tgagcaactg tttttccacc agccgacggt
    16741 aaaactcata ctccagttcc gttatcccta gtgtcgccag ttctggcggc aggagacgta
    16801 aaatatcatc gctttgttgc agaccatcaa cctgctcagg aaccgtcgcc ggttcgcgca
    16861 ccatggtgcg gaaggtttcc atctacgcat cgttgcgcgg tattgatttg gcttcccgag
    16921 aacgccccgg ctgctctgcc aggcgtttca gttccggctg ttcgttaaga aattcaccgt
    16981 atttcacaat aaactgatag tcgccacgtt taagctgacc ggcgctcata tcccacagac
    17041 gaccagctgc ggtattgtta tctgcgagaa tcggttcaag ttgtccgctc agcgtcatgc
    17101 gttcctgaac ttcacttaac agttgttcgc gttcttcttc taatagctgt tgatttaacg
    17161 tcgttgcttg cacgatcaga cttaaacgcc aacgctggag aaaaagcgtg tgtaacgctg
    17221 aagttattgt gctgttagca tcaaccaact gccgggcttg ttctgcccat ggggaattca
    17281 gacgatgcag taagtctagg atctgtggta gctgcacaat aaactgcggc gtggagagga
    17341 gctggctttg ctgatagcac atcacctctt cggtgagttc cggcgggact cgggcatctt
    17401 tcagccgact gcgcagcgcc tcacgccagc ggggaacatc atcagtgatt gccgccttca
    17461 gtcgtgggaa tttttcaaag aagaccgcca gctgcggtga ggccagcagt gcgatgatca
    17521 tctcttcgat caatccctct tcgctgacgg ccagcatcac attaagcgta tccagcgtta
    17581 gcattgttgt gcctggcgaa tctgtgcacc gacatcctgt aggctggctt caatgcgacc
    17641 taaccagtcg ccagggataa acagacattt ttgctgttcg ctgaataaag cgtgttgctt
    17701 acgccagtcg ctttcaagct cttccagttg ttgttttatt tcacctggca gaccttcagc
    17761 cgatgaaccg ggaagtgcca gcgtactgcc ttgtaaactt acatcgcgta caacaagatg
    17821 ttgggtgcta tcaacttcca gattcagttt ctgggcaaag ccgataccgt tcagtttgcc
    17881 gcgaatttca ccacctttac tcagccactg ttccagcgcg ctacgctcaa aggagatatg
    17941 aaccacttcc atatcatgca gttttaacgg tttttgcagc agcagagtca gagtggaagc
    18001 agtaacgtta acaggaagtt gatactgctg acgacggctg aaaatgccgc ccagacgaat
    18061 taacgttaag gctgttttat cgctttgttg ctgctggagt tgcaggtgac gttgcacaat
    18121 cgcgcccagg cgggtcaaca tcccttgctg ttgccaggca tgaccggtca tcaatacatc
    18181 aatttgttgt tgtatcaaat tcaggctttg cgcgtcatac cacaggcaat ctttcagcaa
    18241 aatgagatca accggggcaa cagcactgcg accgctaaaa aaggcgctgg cctgcaataa
    18301 gcggatcgct tttttccagc gacgatccga gacataaggc gcatccggta atttatccag
    18361 ttgctggcgc agcataaaaa tcagctcaaa tacatgatcg ggcagcgtaa tttcaccaat
    18421 ctctttctgc cagcgttcat attcttcatc tgtgacctgc aaggcatcag gaaccggatt
    18481 gtcgttttca tcctgttgac tggtcagcat ggagcggaaa ttcgctttat cttgcacttt
    18541 atctaaccat agacgaatca gcatgcggtc atataacgct tccagactgc tgtctgcttc
    18601 cggcagctcg ttggaggccg ccaccagtag acgcatcggg attttttcta cgtgtgcacc
    18661 gttgcggaac tggcgctcgt taatggcggt gagcaaggta ttaagaattg ccgggcccgc
    18721 tttccagatc tcatccagaa agacgatttc tgcttccggc aggtaaccgc tggttaaacg
    18781 ttcatagcgc ccttcatctt ttagcgcctg aatagaaagg ggaccaaaaa cttcttccgg
    18841 cgtggagaag cgggtcatca gatattcaaa cgcgcgggca ttctgaaagg cgaattttaa
    18901 gcgccgggcg atcaaacttt tggcaatacc tggcgggcca aggaggaaca cactttcacc
    18961 acttaatgcc gctaataaac acaagcggat ggcgtggcta cgttcataaa gccccttttc
    19021 caacgaactg ctcaggcggg aaattctttc cgctaataaa tgagggtgag ccataatgaa
    19081 gtggcgtcct ttcgtcaaaa gttctgcgta aattgcgagt atagacgttt cttgctggtg
    19141 gctaaaatag tctcaaaggg gggtaat
//
LOCUS       DAJTXY010000075        19130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTXY010000075 DAJTXY010000000
VERSION     DAJTXY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.075"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(47..139)
                     /locus_tag="OO566_002657"
     tRNA            complement(47..139)
                     /locus_tag="OO566_002657"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Ser,seq:gct)
     gene            complement(455..640)
                     /gene="csrA"
                     /locus_tag="OO566_002658"
     CDS             complement(455..640)
                     /gene="csrA"
                     /locus_tag="OO566_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6605887.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(875..3505)
                     /gene="alaS"
                     /locus_tag="OO566_002659"
     CDS             complement(875..3505)
                     /gene="alaS"
                     /locus_tag="OO566_002659"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6605888.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3633..4133)
                     /gene="recX"
                     /locus_tag="OO566_002660"
     CDS             complement(3633..4133)
                     /gene="recX"
                     /locus_tag="OO566_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6605889.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4376..5437)
                     /gene="recA"
                     /locus_tag="OO566_002661"
     CDS             complement(4376..5437)
                     /gene="recA"
                     /locus_tag="OO566_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6605890.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="OO566_002662"
     CDS             complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="OO566_002662"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6605891.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="OO566_002663"
     CDS             complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="OO566_002663"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6605892.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7500..8063
                     /gene="srlA"
                     /locus_tag="OO566_002664"
                     /gene_synonym="gutA"
     CDS             7500..8063
                     /gene="srlA"
                     /locus_tag="OO566_002664"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR6605893.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8060..9019
                     /gene="srlE"
                     /locus_tag="OO566_002665"
                     /gene_synonym="gutE"
     CDS             8060..9019
                     /gene="srlE"
                     /locus_tag="OO566_002665"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6605894.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9030..9401
                     /gene="srlB"
                     /locus_tag="OO566_002666"
                     /gene_synonym="gutB"
     CDS             9030..9401
                     /gene="srlB"
                     /locus_tag="OO566_002666"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6605895.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9405..10184
                     /gene="srlD"
                     /locus_tag="OO566_002667"
     CDS             9405..10184
                     /gene="srlD"
                     /locus_tag="OO566_002667"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6605896.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10289..10648
                     /gene="gutM"
                     /locus_tag="OO566_002668"
     CDS             10289..10648
                     /gene="gutM"
                     /locus_tag="OO566_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6605897.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10715..11488
                     /gene="srlR"
                     /locus_tag="OO566_002669"
                     /gene_synonym="gutR"
     CDS             10715..11488
                     /gene="srlR"
                     /locus_tag="OO566_002669"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6605898.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11481..12446
                     /gene="gutQ"
                     /locus_tag="OO566_002670"
     CDS             11481..12446
                     /gene="gutQ"
                     /locus_tag="OO566_002670"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6605899.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSSTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12443..13957)
                     /gene="norR"
                     /locus_tag="OO566_002671"
     CDS             complement(12443..13957)
                     /gene="norR"
                     /locus_tag="OO566_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6605900.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGNDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14144..15583
                     /gene="norV"
                     /locus_tag="OO566_002672"
     CDS             14144..15583
                     /gene="norV"
                     /locus_tag="OO566_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6605901.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15580..16713
                     /gene="norW"
                     /locus_tag="OO566_002673"
     CDS             15580..16713
                     /gene="norW"
                     /locus_tag="OO566_002673"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6605902.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16841..19093)
                     /gene="hypF"
                     /locus_tag="OO566_002674"
     CDS             complement(16841..19093)
                     /gene="hypF"
                     /locus_tag="OO566_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6605903.1"
                     /translation="MAKNTFCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
BASE COUNT         4494 a         5322 c         4890 g         4424 t
ORIGIN      
        1 tcggttcgta gccgagtact ctatccagct gagctacgga tgcaaatggc ggtgaggcgg
       61 ggattcgaac cccggatgca gcttttgacc gcatactccc ttagcagggg agcgccttca
      121 gcctctcggc cacctcacca cacgcctctt acgagtgctt cgaagaactt gtttatcgct
      181 catcgtcgct gcgtggcgca catattactt tctgagactt ataagtcaaa caatttttcc
      241 cacactttta tcgtttgcac acttcacgct caattagtct gtaaaaacgt caaaaagagt
      301 gttttatcta cagaaaaacg gaggtctggc agatagcagt aatgcaaaaa aatggagact
      361 taagttgaat gaacgggagt aaagcgaaaa gactatagag tgaaggagaa attttgaggg
      421 tgcgtctcac cgataaagat gagacgcgga aagattagta actggactgc tgggattttt
      481 cagcctggat acgctggtag atctcttcac ggtgaacaga aacttccttc ggggcattta
      541 cgccaatacg tacctggttg ccctttaccc ctaaaactgt cacggtgacc tcatccccaa
      601 tcatgagggt ctcaccaact cgacgagtca gaatcagcat tctttgctcc ttgaaagatt
      661 aaaagagtcg ggtctctctg tatcccggca ttatccatca tataacgcca aaaagtaagc
      721 aacgacaaac acattacatg taagcagtca tggcattaca ttctgttaaa cctaagttta
      781 gccgatatac acaacttcaa cctgacttta tcgttgtcga tagcgttaat gcgaatgccg
      841 tgaagcgagt ccacggcatt gcctgacgct tatattattg caatttcgcg ctgacccagc
      901 ctttcacact ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca
      961 tgtcaggacg tccaccaccc ttgccgccca cctgctgagc gaccatacca atcagttccc
     1021 ctgctttcac acgatctgtg acgtccttag atacgcctgc aatcagagaa accttacctt
     1081 cggctaccgt tgccagcacg ataattgtcg accccagctg attttttaaa tcgtcaacca
     1141 tggtacgcaa cattttcggc tcaacaccgc taagctcgct aaccaacagc ttaacaccat
     1201 taacatcaat tgccttactg gaaagatttg cgctctcctg tgcggcagct tgttctttaa
     1261 gctgttgtag ttctttttct aactgacgcg tacgttccag tactgagcgc actttatccg
     1321 ccagattatt gctatcgcct ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg
     1381 catgaacggt ggtgatagcg ccttctccgg ttaccgcttc gatacgacga acgcctgcag
     1441 cagtacccga ttcagagatg atgcggaaca gaccaatatc accagtgcgg ctggcgtgag
     1501 taccgccaca cagctcggtg gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat
     1561 acttctcgcc gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga ttcatgatgt
     1621 tggtttcgat cggcaaattg cgacgaatct gtgcgttcac caggtcttcg accgcacgaa
     1681 tctcttccgg tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa
     1741 ccagtgaacc tttctgcgat acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca
     1801 ggtgcgttgc ggagtgattc agacgaatac gggcgcgacg agcctcatca atatcagcct
     1861 gcacagcgtc gcccactttc agagaacccg cagcaagttt accgatgtga ccaatcgcct
     1921 ggccgtattt ctgcgtatct tccaccgcga aggagaagtt agcgcctttc agttcgcctt
     1981 tatcaccaac ctgaccgccg gattccgcat agaatggcgt ttgatccagc acgaccacag
     2041 cttcctggcc tgcattgatg gcatcaaccg ctttaccatc aacaaacagc gcggtcactt
     2101 tgccgttcag ttccagatgg tcatagcctt taaattcaga tgcactatca acacggatca
     2161 ttgcgttgta atcggcacca aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca
     2221 ttgctgcttc aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg
     2281 tcaggtcaac cgggaagcca taggtgtcgt acagacggaa agccgtttca ccatccagcg
     2341 tatcaccaga aagttttgcc agctcttcat ccagcaacgc cagaccgcgc tccagagtac
     2401 gggcaaactg ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca
     2461 ggtcttcacc cgcagagccc ataacgtcga tcagcggacc aaccagtttg tagaagaagg
     2521 tttctttcgc gccgagcata ttgccgtgac gcactgcgcg acgaatgata cgacgcagta
     2581 cataaccacg gttttcattg gacggcatta cgccatccgc gatcaggaac gcacaagaac
     2641 gaatgtggtc agcgattacg cgcagcgatt tattgctcag atcggtcgcg ccagtgactt
     2701 tcgctaccgc ctggatcaac gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt
     2761 gcagcaccgc agcaatacgc tccagaccca taccggtatc tacagacggc ttcggcagcg
     2821 gttccatcgt gccatctgcc tggcggttga actgcatgaa gacgatgttc cagatctcaa
     2881 tgtagcggtc gccgtcttct tccggacttc ccggagggcc accccaaatg tggtcgccgt
     2941 gatcgtagaa gatttcggtg cacgggccgc acggaccagt gtcacccatc tgccagaagt
     3001 tgtcagatgc gtatggcgca cctttgttat cgccgatgcg aataatacgt tcgcgcggga
     3061 tcccgacttc tttttcccag atttcgtagg cttcgtcgtc gctttcatag acggtaaccc
     3121 acagacgctc tttcggcagg gcaaaccatt tttcgctggt cagcagttcc catgcaaact
     3181 gaatggcatc gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt tcgaagaagg
     3241 tatggtgacg cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca
     3301 cgcagcgttg ggaagtggta gcgcgggaat aattacgctt gtcgagccca aggaacacat
     3361 ccttgaactg gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta tggggtacca
     3421 gggagctgct ggcaactacc tgatgtccct tactatggaa aaagtcgaga aacgcctgac
     3481 ggatctcagc ggtgctcttg ctcataatta tcctgaaatc aagctaacga aatatcgcca
     3541 ccagctccag cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat aagataagtt
     3601 ttcttgactg ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc
     3661 tggatatctt ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct
     3721 gaaaagacag ttggcaaagg ttcaccatat tttcgcgtcg cctgatcgcg cgccagtgcg
     3781 caccagtcga tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc
     3841 tgattcagtt cctgacgaat acgtgcaggt ccataacctt tgcggctacg gctggcgata
     3901 aagcgcgcaa caaatcggct gtcatcgaga tagccatgtt catggcacca ggcaataacg
     3961 cgctcgtaat cttctgccgt agcatcaatc tcttctgggc catttttgcc cataatcggt
     4021 gccgcgagtt tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc
     4081 gcacgatcca acaggcgagc atatgccggg cgacgggatg ttgattctgt catggcatat
     4141 ccttacaact taaaaaagca aaagggccgc agatgcgacc caggttttgc tgataaacag
     4201 atttaatagt caatgagtat gcagaatgtt cccgcaggcc caattcgtta gaggatcgag
     4261 gtgagagtaa tttttcacgc cagtgctggc ctgttcccct caccctaacc ctctccccaa
     4321 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
     4381 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4441 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4501 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4561 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4621 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
     4681 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4741 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4801 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     4861 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4921 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     4981 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     5041 gggcgtcaca gatttccagt gcctgctcgc cggtatccgg ctgggagcac agcaggttat
     5101 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5161 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
     5221 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5281 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5341 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5401 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5461 ataccggata gtcaatatgt tctgttgaaa caattatact gtatgctcat acagtatcaa
     5521 gtgttttgta gaaattgttg ccacaaggtc tgcaatacat acgcagtagc ctgacgacgc
     5581 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
     5641 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5701 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5761 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     5821 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
     5881 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
     5941 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     6001 tcactgtcag tcatcacaac ctctgtcagt caaaaattta tgccggacaa gatagcactt
     6061 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6121 tagccagagg gaagctcacg cccccctctt gtaaatagct actgtactcg cgccagcgcc
     6181 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt gtaacgggtg
     6241 atggtgtaga agttcggcag accgtaccag tactggtagc cggtgccaac atccagacgc
     6301 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgcggcggca
     6361 agctgcgaaa tgctgtactt agttttgaaa ccatttggca agcctggagc ctgaccgttt
     6421 gccattatcg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccacg
     6481 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6541 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6601 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcagcaggaa ggtttccagc
     6661 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6721 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6781 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6841 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6901 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     6961 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
     7021 cgatcgaaac cgtgtttgtt caccattttg tcgatgaact gctgggcatt cgggttatta
     7081 gcgaaatcgc cgcccatctg catcacattg tgctgcggct caagcaggaa gccgccagac
     7141 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7201 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7261 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7321 ctgcccgata cggactttac ataactcaac tcattcccct cgctatactt ttactcaaac
     7381 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaacctttca
     7441 acctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7501 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7561 aggtgtttac cggaatggtg accggcattc ttccgctgtt gattagcctg ctggttatca
     7621 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7681 ccggtaaccc tgtttcccgt tacctactgt taccgtgcat tggcacgttt gtcttttgca
     7741 atccgatgac cttaagccta ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7801 cggcggcctc ttatagctgc cactcaatga atggcctctt cccccatatc aaccctggcg
     7861 aactgtttgt ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc
     7921 cactggcggt gagttatctg ctggttggtc tggtcaccaa tttcttccgc ggctgggtga
     7981 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
     8041 acctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
     8101 cggcccgctt gagctggaag ctacgccggg caaaaaaatc gtctatatca ccgccggtac
     8161 ccggcctgcg attgttgaca aactggcaca acttactggc tggcaggcta ttgacggatt
     8221 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8281 attacgctgc ggcatctatc cgaaacgacg tattcccacc attaatatcc actcgacggg
     8341 caagtccggt ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8401 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8461 tgacaccagt aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8521 catggggtcc gccgttgccg tgttgtttca gtctggtcgt gacaccatcg acactgtatt
     8581 aaaaaccatt ctgccgttta tggcattcgt ctcggcgctc attggcatca ttatggcttc
     8641 tggccttggt gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     8701 ggtcatgctg gcgctcatct gctccttccc actgctttca cctttcctcg gcccaggcgc
     8761 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     8821 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     8881 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     8941 tgtactggtg agccgctttt taaccggcgc gccgactgta ctgatcgcct ggtttgtctc
     9001 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9061 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9121 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9181 cactccatcc cggtttgcaa ttttcactcg ggcagcatcg ctatccggtg accgctgttg
     9241 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9301 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9361 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9421 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9481 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9541 ttaacgccga atatggtgaa ggtatggcgt acggttttgg tgctgacgcc actagcgagc
     9601 aaagcgttct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9661 tctatagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9721 accgttcgct acaagtgaat ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt
     9781 tgatgatccg cgacgggatt cagggtcgca ttattcagat caactcgaaa tccggcaaag
     9841 tgggcagcaa acacaactct ggctacagcg cagcgaaatt tggtggcgtc gggctgactc
     9901 aatcactggc gctggatctg gcggagtacg gcattacggt gcattcactg atgctcggta
     9961 acctgctgaa atcgccgatg ttccagtcac tgttgccaca atacgcgacc aagctgggta
    10021 tcaaaccgga tcaagtcgag cagtattaca tcgacaaagt accgctcaaa cgcggctgcg
    10081 attatcaaga tgtgctgaat atgctgctgt tctacgccag tcctaaggcg tcgtactgca
    10141 ccggacagtc gatcaatgtc accggcggtc aggtgatgtt ctgatcaaca gcggagatcc
    10201 gttaaggatc tccgtgagac tatagaatgc ctgatgcgct acgctcatca ggcatacagg
    10261 acttccgcca ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat
    10321 cgcctggtgt gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt
    10381 cgacacacta tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc actttaaacc
    10441 gcgggtcgtg gtcgccatcg cgctggacga tcagcagtgc atcgtcgaca ccttgtttat
    10501 gaaaggactg actgtcttcg cccgaccgca aaaaattccc gcaattaccg gcatgcatgc
    10561 gggtgattta cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc
    10621 attggcgctt aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt
    10681 atcattttga aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc
    10741 cattctggag tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt
    10801 tgacaccaca ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg ccggaaccgt
    10861 cattcgtact tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca
    10921 taaaacgctc atcaacaccc acaagaaaga gctgattgca gaagctgccg ttagttttat
    10981 ccatgatggc gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggttcccct
    11041 gctctcgcgc tttaataaca tcacggtgat gaccaacagc ctgcatatcg tcaatgcgct
    11101 atccgaactg gataacgaac aaactatcct gatgccaggc ggaacatttc gcaaaaaatc
    11161 ggcctcattt cacgggcaac tggcagagaa tgccttcgag catttcacct tcgataaatt
    11221 gtttatgggc accgacggca tcgatctcaa tgcgggcgta accacgttca acgaggttta
    11281 taccgtcagt aaggcaatgt gcaatgccgc gcgcgaagtg attttgatgg cggactcatc
    11341 aaagtttggc cgtaaaagcc ccaacgtagt ttgcagtctt gaaagcgtcg ataagctaat
    11401 taccgacgca ggtatcgatc cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt
    11461 gatcataacc ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa
    11521 tgctggagtt gcaggaagca agccgtttac cggaacgtct gggcgatgat tttgttcgcg
    11581 ccgccaatat catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg
    11641 gccacattgg taagaaaatc gccgcaacac ttgccagtac cggcacaccg gctttttttg
    11701 tccatccggc agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc
    11761 tgtttatctc ttactccggt ggcgcgaagg aactggatct gattattccg cgtctggaag
    11821 ataaatctat cgcgctgctg gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg
    11881 cgaaagcggt gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga
    11941 cctccagcac cgtcaatacc ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg
    12001 cgcgcggttt taatgaagaa gattttgccc gctcccaccc agctggggca ctgggcgctc
    12061 gcttgctgaa taaagtgcat catctgatgc gtcgtgatga cgctatccca caggtagcgt
    12121 taaccgccag cgtgatggat gcgatgctgg aactcagcag caccggtctg gggctggtgg
    12181 cggtatgtga cgctcaacaa caggtacaag gcgtctttac cgacggcgat ttacgtcgct
    12241 ggctggtagg cggcggcgca ctcaccacgc cagttaatga agcgatgacg acaggcggca
    12301 ccacattaca ggcacaaagt cgcgccatcg acgccaaaga agttctgatg aaacgcaaaa
    12361 tcactgccgc accagtggta gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg
    12421 atttctatca ggccgggatt atttaatcct tcaatcccag acgtttcgcc agccgatgca
    12481 ggttggcgac gtcagtttcc agcattcgcg cacaggctgc ccagttgtga tgattttgtg
    12541 ccagtgcctg gcgaatagtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct
    12601 taacaacggg caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat
    12661 gttgcgcctc aagaatcact tcatcgccgt tgcgggtggc tctcgccagc actaccgccc
    12721 gatgaatagc atgttccagt tcgcgcacgt ttcccggaaa actgtagtgt tgcagtaaat
    12781 ttcgcgcccc ggcacttaat accacgcggg agagcccctg ccgcaaacga cactgctcgc
    12841 agaaataccc cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcaccgaaa
    12901 gtggaaacac gctcaggcga tgaaacaaat cggcgcggaa tcgccctgcc agcacctctt
    12961 cgcgtaagtc gcggttagtc gccgccagca cgcgcacatc gacccgcaaa ctacggtcat
    13021 tgccaacgcg ctgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca
    13081 acgacaactc gccgatctca tcgagaaaca gagtaccgtt atccgccatt tcaaacttcc
    13141 cgctgcggtt actgatagcg ccagtaaacg ctcctttcac atgcccgaac aactcacttt
    13201 ccgccacact ttccggcagt gcggcacagt tgagatagac cagcggattc accgcccgtg
    13261 gcgaggcttc atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctga
    13321 tcagaacgtt gagatcggac gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc
    13381 caggggacaa gccaatcatc tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg
    13441 gcagcatatt ctggctttcc agttgttcaa tcagcaacgc attgcttaac gctcccgccg
    13501 ccagcgcggc aatcagccgt aactcttcgt cgctgaaaac atcgaactga tcgggctgca
    13561 tcccgtcgag cgtcagtgcg ccgatcaggt tttgtccgac aaacaatggc agaccaacgc
    13621 aggcgtgaac cttcagactc tcctgcccag gaatcaaacc gtcataggga tcgggcaatt
    13681 cgctgtctgc gggaaagcgc accacatccc cggcgcgggc aatcgcttcc agccgtggat
    13741 gcccttccag cgcaaagcgt ctaccgagta catcctttgc cagaccgtcg atggcaagcg
    13801 gaataaactg ccgcgaatcg taacgtagca acgcagacgc atcgcactcc agcacctgac
    13861 gtagcgtggt gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga
    13921 tggcgatatt cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac
    13981 tatagatatt gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatgagc
    14041 ataattttat ttatagagta aaaacaatca gataagaaac tggcacgcaa tctgcaatta
    14101 gcaagacatc tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa
    14161 aaataacatt cattgggtgg gtcaacgtga ctgggaagtg cgtgattttc acggcacgga
    14221 atataaaacg ctgcgcggca gcagctacaa cagctacctc atccgcgaag aaaaaaacgt
    14281 gctgatcgac accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga
    14341 aatcgatctg gcggatatcg attacatcgt gattaaccat gcagaagagg accacgccgg
    14401 ggcgctgacc gaactgatgg cgcaaattcc cgatacgccg atctactgta ctgccaacgc
    14461 tatcgactcg ataaatggtc atcaccatca tccggagtgg aattttaatg tggtgaaaac
    14521 tggcgacacg ctggatatcg gcaacggcaa acagctcatt tttgtcgaaa caccaatgct
    14581 gcactggccg gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga
    14641 tgctttcggt cagcactact gcgacgagca tctgttcaac gatgaagtgg atcagacgga
    14701 gcttttcgag cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt
    14761 aacgccgaaa attaccgaga tcctgggctt taacttacca gtcgatatga tagcgacctc
    14821 ccacggcgtg gtctggcgcg ataacccgac gcaaattgtc gagctgtacc tgaagtgggc
    14881 cgctgattat caggaagaca gaatcaccat tttctacgac accatgtcga ataacacccg
    14941 catgatggct gacgctatcg cccagggaat tgcggaaacc gacccacgcg tggcggtgaa
    15001 aattttcaac gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa
    15061 aggcgtgctg gtcggcactt cgacgatgaa taacgtgatg atgccgaaaa tcgccgggct
    15121 ggtggaggag atgaccggat tacgcttccg taacaaacgc gccagtgctt tcggatctca
    15181 cggctggagc ggcggtgcgg tggatcgcct ttccacgcgc ctgcaggatg cgggtttcga
    15241 aatgtcgctc agcctgaaag ccaaatggcg accagaccag gacgctctgg agttatgccg
    15301 tgaacacggt cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt
    15361 gaatacggta gttaaagaag aaacctctgc cacgacgacg gctgacctcg gcccacgaat
    15421 gcagtgcagc gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt
    15481 tgcgccagga acgccgtgga gtgaagtccc ggataacttc ctctgcccgg aatgctccct
    15541 cggcaaagac gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc
    15601 atcggttcgg gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccact
    15661 attccattaa ccctgattgc cgccgacagc atagatgagt acaacaaacc cgacctcagc
    15721 catgttatca gtcaggggca acgtgccgac gaccttaccc gccagacggc gggtgaattt
    15781 gccgggcagt ttaatctgcg cctgtttccg cacacctggg tgacggatat cgatgccgaa
    15841 gcccatgtgg tgaaaagcca gaataatcag tggcaatacg acaagttagt gctggcaacc
    15901 ggtgccagcg cctttgtccc gcctgtgcct gggcgtgagt taatgctgac gttaaatagt
    15961 cagcaagagt atcgcgcctg tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc
    16021 ggcggtggtt tgattggtag cgaactggcg atggattttt gtcgtgcagg caaagcggtc
    16081 acgctaatcg acaacgctgc cagtattctg gcgtcattaa tgccaccaga agtaagcagc
    16141 cgcttgcagc atcggttgac ggagatgggc gttcatctgc tgttgaaatc tcagttgcag
    16201 gggctggaaa aaacggattc tggcattctg gcaacgctgg accaccagcg tagtatcgaa
    16261 gtggatgcgg taattgctgc caccggactg cgcccggaaa cagccctggc acgacgcgcc
    16321 gggctgacga ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgccgat
    16381 atttacgcgc tgggcgattg cgcggaaatt aacggtcagg tattgccgtt cctgcagccg
    16441 attcaactta gcgggatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa
    16501 ctaccggcga tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa
    16561 acccagcgtc aggatttacg ctggcagatt aataccgaac gccagggaat ggtggcgcgc
    16621 ggcgttgacg atgctgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag
    16681 gcatttgtat tgttgaaaac gttgccggtg taggtgggct actgtgccta aaatgtcgga
    16741 tgcgacgctg gcgcgtctta tccgacctac ggggacgcat gtgtaggccg gataaggcat
    16801 ttacgccgca tccggcaagg gtgtccaaat gcaacacgct ttatccgttc tggacttcac
    16861 ccgctaacca acgcgccgca gcaataaccc cctgccccag agacaaaccg ccatcacccg
    16921 ccggtaaact ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc agacgtgcac
    16981 gcagcaaacg gttatgaata accccgccgc taaataccag cgtagtgata ccacgcatcg
    17041 tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg
    17101 cgcgttgatt aaccggtgcc tgccagttca gccactgctg ccagaaagtg gcgagatcca
    17161 gttgattgtc cacccgcggc atcgtcaccg gatgcgtcac tccgtcgcat gaggctgcga
    17221 gcgcctccag agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca
    17281 gtgccgccgc cactgcatcg aacaaccgcc cacacgatga tgccagcggc gcgttaattc
    17341 cacgctcaat ggcccgcgcc agtacgctcc agttttgctg ttgcacactt gccgtttcag
    17401 ggtaattctg ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct
    17461 gcttcgccgc caaatcgcca cccggaagcg ccactgcagg caagccgccc aggtgctcac
    17521 attcgcgata gttcacccgc aggcactcgc cgccccacaa agcgccattc tcccccatac
    17581 cgataccgtc gagcgtcaaa gcaatgacat caccgccatc cagcggccac tgatgctctg
    17641 ccagacacgc cgctgcatgg gcatgatgat gcagtaccgt ttgcgtcggc agattcattt
    17701 cgctcgccca ctggctggag acatagcccg gatgcacatc atgcacaatg tattgcggag
    17761 tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat
    17821 cgtcacttaa atcgcccaga tgctgactca acaccacttg ttcaccgcgc accaggcaga
    17881 aggtattttt cagatccgcg ccaagacaca gcacaggcgg aacattttta aagcccggag
    17941 gcaaaaccag cgcatccggc acataccccc gcgaacggcg cagcatttcg ccgctttcgc
    18001 gcaccactga atcatccatc cgctgcacga tgtcgcggtt atgtagcaag aatccgtcgg
    18061 caatgccctg caaatccgcc agcgcctgtt cgttgctgat agctggtggt ttaccgctca
    18121 ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg
    18181 ggttcgcagg caacatcacc ccgacttcgt taaggccagg ggcgatatca tcacaaagct
    18241 caggtacgta ttttttatcc accagcacaa tcggcgcagc gggcgtggta agcaactggc
    18301 gcgcagcgtc tggtaaactt tctgccactg gcaacatgac tgccagcggt ttcgccgggc
    18361 gatgtttgcg ctcccgcagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat
    18421 gaaatccgcc aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagctg
    18481 cctgtaatgc cgcctcttgt tctgcatgtt caccatgact tacccattca agatgcgggc
    18541 cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcgcggtact
    18601 ctttgtcaca ggccggacat agcggaaacg ccgccatcac ggtaaacggg cggtcgtaag
    18661 gcatcgcgcg aataatggtg aaacgcgggc cgcagtgggt acagttgata aacggataac
    18721 gataacgccg ctcgcctggg gtattcattt cggcaaggca agcagggcaa gtggcggcat
    18781 ccgggacaat ttgcgtattc atggtgccgc cggtgctctg gcggatggtg aactcggtgg
    18841 gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc agtggcggac
    18901 agtgctgatg caattgaaca agaaacgttt ccggatcttc cagcagccgg acttctacgc
    18961 catcgccgtc attacataca tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa
    19021 acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaaaatg
    19081 tgttttttgc cattgagtta ttcccgccat catgaattgc gtaacccgcc
//
LOCUS       DAJTXY010000076        18261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTXY010000076 DAJTXY010000000
VERSION     DAJTXY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.076"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="OO566_002675"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="OO566_002675"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6605904.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="OO566_002676"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="OO566_002676"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6605905.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3000)
                     /locus_tag="OO566_002677"
     CDS             complement(1729..3000)
                     /locus_tag="OO566_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6605906.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3183..4387)
                     /locus_tag="OO566_002678"
                     /pseudo
     CDS             complement(3183..4387)
                     /locus_tag="OO566_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4487..4843)
                     /locus_tag="OO566_002679"
                     /pseudo
     CDS             complement(4487..4843)
                     /locus_tag="OO566_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="OO566_002680"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO566_002680"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OO566_002681"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="OO566_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6605907.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAILAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="OO566_002682"
     CDS             6773..9829
                     /locus_tag="OO566_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6605908.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVVLIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="OO566_002683"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="OO566_002683"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6605909.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="OO566_002684"
     CDS             10336..10524
                     /locus_tag="OO566_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6605910.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="OO566_002685"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="OO566_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6605911.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="OO566_002686"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="OO566_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6605912.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="OO566_002687"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="OO566_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6605913.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="OO566_002688"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="OO566_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6605914.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="OO566_002689"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="OO566_002689"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6605915.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQAQGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="OO566_002690"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="OO566_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6605916.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="OO566_002691"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="OO566_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6605917.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="OO566_002692"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="OO566_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6605918.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4808 a         4403 c         4689 g         4361 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccagcca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcttgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtaaccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaacgg tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgccag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccatgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgcactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagctatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgccaaa aaagaaggcc
     4141 atatagcagc acattccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgaca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaataaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttactgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcagcg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcga ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aagtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg gacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgtccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggtgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gctagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggtctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gcatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaaatcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac tttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagct atcgcggcca ggaaacgcac gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gcttaaacag caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgcagg gttgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggat cgtctggtga ccggcctgca acgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgacctgc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcggcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt g
//
LOCUS       DAJTXY010000077        17572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTXY010000077 DAJTXY010000000
VERSION     DAJTXY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.077"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(267..1295)
                     /gene="epmB"
                     /locus_tag="OO566_002693"
     CDS             complement(267..1295)
                     /gene="epmB"
                     /locus_tag="OO566_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6605919.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1337..1903
                     /gene="efp"
                     /locus_tag="OO566_002694"
     CDS             1337..1903
                     /gene="efp"
                     /locus_tag="OO566_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6605920.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1955..2080
                     /gene="ecnA"
                     /locus_tag="OO566_002695"
     CDS             1955..2080
                     /gene="ecnA"
                     /locus_tag="OO566_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6605921.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            2191..2337
                     /gene="ecnB"
                     /locus_tag="OO566_002696"
     CDS             2191..2337
                     /gene="ecnB"
                     /locus_tag="OO566_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6605922.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2513..2742
                     /locus_tag="OO566_002697"
                     /pseudo
     CDS             2513..2742
                     /locus_tag="OO566_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2739..3272)
                     /gene="blc"
                     /locus_tag="OO566_002698"
     CDS             complement(2739..3272)
                     /gene="blc"
                     /locus_tag="OO566_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6605923.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3361..4494)
                     /locus_tag="OO566_002699"
     CDS             complement(3361..4494)
                     /locus_tag="OO566_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6605924.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="OO566_002700"
     CDS             complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="OO566_002700"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6605925.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="OO566_002701"
     CDS             complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="OO566_002701"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6605926.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5333..6067)
                     /gene="frdB"
                     /locus_tag="OO566_002702"
     CDS             complement(5333..6067)
                     /gene="frdB"
                     /locus_tag="OO566_002702"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6605927.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="OO566_002703"
     CDS             complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="OO566_002703"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6605928.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            8193..9170
                     /gene="epmA"
                     /locus_tag="OO566_002704"
     CDS             8193..9170
                     /gene="epmA"
                     /locus_tag="OO566_002704"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6605929.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9389..10891
                     /gene="yjeM"
                     /locus_tag="OO566_002705"
     CDS             9389..10891
                     /gene="yjeM"
                     /locus_tag="OO566_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR6605930.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="OO566_002706"
     CDS             complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="OO566_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6605931.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSIAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14375..15343)
                     /gene="psd"
                     /locus_tag="OO566_002707"
     CDS             complement(14375..15343)
                     /gene="psd"
                     /locus_tag="OO566_002707"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6605932.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="OO566_002708"
     CDS             complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="OO566_002708"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6605933.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNIKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDG"
     gene            16587..17132
                     /gene="orn"
                     /locus_tag="OO566_002709"
     CDS             16587..17132
                     /gene="orn"
                     /locus_tag="OO566_002709"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6605934.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17343..17418
                     /locus_tag="OO566_002710"
     tRNA            17343..17418
                     /locus_tag="OO566_002710"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17376..17378,aa:Gly,seq:gcc)
BASE COUNT         4070 a         4520 c         4612 g         4370 t
ORIGIN      
        1 aaaggttaat tattggtgtt agctatataa aaagtaacaa cagcaatgca tatgaataat
       61 taaaggttat gcaacgtgca aagattaaat ttctcagttt aatgacaggt tgctgtactt
      121 gcgtttgttg ctcttgccgg atgcggcgta aacgccttat ccggcctaca gttcattgca
      181 aatccattca atttcaatgc acgatgtagg cctgataagc gtagcgcatc aggcaatttt
      241 aatgtttaac ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt
      301 ttgctgggtt cgccgccaat ttctcgcgcc agtttcggca ccagatatcc cgacaccaat
      361 gtcagcaact cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg
      421 ccctgtactt tatctagcac atgcaggtaa tacggcatca cgccggcatc gaacaacgca
      481 ttactcaggt ttgccaacgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg
      541 ttaagcaggg tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct
      601 acctcattgg catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag
      661 cgttcaacca gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc
      721 cgcagacgtt tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga
      781 tctttcgcca tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca
      841 gcaacatact caagtgcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg
      901 aagtggcgac ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc
      961 gcccggttgt ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagtggg
     1021 tcggtggaga atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa
     1081 agaggatcgt ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg
     1141 gcaaacagct ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa
     1201 agacgcagaa gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct
     1261 ctggatgggg tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca
     1321 aatttcagag ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat
     1381 catgttagac ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg
     1441 ccaggcattt gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac
     1501 cttcaaatct actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct
     1561 gtacaacgac ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc
     1621 tgatgcaaaa gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt
     1681 aactctgtgg aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat
     1741 cgttgatacc gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac
     1801 cctgtctact ggcgctgtgg ttaaagttcc gctgttcgta caaatcggcg aagtcatcaa
     1861 agtggatacc cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc
     1921 ctgcaggctg caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt
     1981 tttgcttgcc agcacgctgc tcacgggctg taacaccgct tgcggtttcg gcgaagacat
     2041 caaacatctc ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa
     2101 atcatcagat ttccatcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac
     2161 ataaataaca ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt
     2221 tctgttctgg tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa
     2281 gacatttctg atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaataagca
     2341 ataacggtac gacagcagtg tcgtgccgtt tgttttttct gcgatcgtca taaaggtaat
     2401 agttgaaatt cccctgccac ctggcaaaat atccgttcaa ccatcagctt tgcaggacga
     2461 cctgcaaacg cctcttttca ccggggacgg ccccaaattt ccggagcctg atatgtcctg
     2521 gattatctta gttattgctg gtctgctgga agtggtatgg gccgttggcc tgaaatatac
     2581 ccacggcttt agccgtttga tgccgtgtgg acgggtattg gcgcagtcgg cgcggccatc
     2641 accggcattg tgctgctcgg tgagtccgct aacccgatgc gtctggcgag tctggcgtta
     2701 atcgtattgg ggattattgg tctgaaactc agcactcact aactaccggg ctgctgtacc
     2761 caaataaact tactgacatc aaacccttcc cgggtcgcga ctgccagcat ctcctgtttc
     2821 acttcgtcag aaatggttgg cgtgcgggag agtatccaca ggtagtcgcg gtccgggccg
     2881 caaaccagcg catggcggta ttcccgatcg agtgcaataa cgttataacc gccatagaaa
     2941 ggaccaaaga atgacacttt cagcgcagcg cgagttggtg cgccggtaaa gtacgctttc
     3001 ccttcactct gctgccacat tcctctgtca gggttatagc ctttattaat gacattcagg
     3061 ccgccgtcat cacgcaggct gtatgttgcg gtgacttttt ccagtccacg ttcaaaacgg
     3121 tgatcaaaac gggcaatctc ataccaggta ccgagataac gtttggcgtc gaaattattc
     3181 actacggtca cgccacgcgg cggcgtagga gaactacagg caacgaccag aaatgcagct
     3241 gtcgctgcgg caacgagagg gagcaggcgc ataaatgttt ccttactggt ttttttctaa
     3301 gtgtagatga cagcaaggaa aagcgcagaa aaggtccgaa aattcggacc cgatgaaatt
     3361 ttactgtaga gcgttgagaa tctgacaggc ggcggcgact ctctctggat tgggatagtt
     3421 tttgttagcc agcatcacga tacccagctc tttttctgga ataaacgcga catagctacc
     3481 aaatccgccg gtcgcccccg ttttatgtac ccatgatgcg cgtactgcag gagttggggg
     3541 cttaatcgct tttaccgggc gtgctgccag tgcaatttta ttgtcactgc cgttaatgat
     3601 gctgtcagga ttgaccggcc agtccagcat ttcccagccc aggccctgat acatatcgcc
     3661 ggtttgccag tagcgagatt gtgccagttg tatcccttgt tgaagcgttt tctcattgat
     3721 atcaaggggg ggtaaattgc tttgcaccca gcgggccata tcttcaatgg tcgacttcac
     3781 accataagct tcagcatcta acgcccctgg cgaaacatgc actgccttac cttcgcgata
     3841 tccccaggcg taattctttt cttctggggg aggtacatta atccacgtat ggttgagttt
     3901 gagtggctgg aagacacgag tttgcatcgc ctgctcaaaa ctcaaaccag acggcttcac
     3961 agccagtgcg ccgaacaaac cgatactgga gttggcatac agacgttgtg ttcctggggc
     4021 ccatgcaggc tgccagtttt gatagaagcg cagcaagtcg cttgaggatt tcacttcatc
     4081 cggcacctgc aatggcaggc cgccagcggt gtaggtcgca agatgtaata gtgtgatccc
     4141 attccactgt ttagcggtaa gttcaggcca gtattttgtt gcgggatcgc ttaacttgat
     4201 ttcccctcga gcaatagcgt cgccaccaag cacgcccgta aatgttttgc tgaccgaacc
     4261 taactcaaac aacgtttgct gtgtgacggg ctgctttttg gcgatgtccg catagcccca
     4321 agtaaagtaa taaggtttac cctgataaat taccgccacc gccataccgg ggatcttttg
     4381 ttgctctata agcggggtaa ttgtgcgatg cacaatatcg ttgatttgtt gaggggcagc
     4441 aaatgtggag caagaggcgg taattaataa ggcgcagagc gtcgttttga acataaggtc
     4501 tggtttccat acaaaacggc ccgccatagg cgggccggat ttacattggc gatgcgttag
     4561 attgtaacga caccaatcag cgtgacaact gtcaggatag cagccagacc gtagaaaacc
     4621 catttgcccg caggcacgtg gattttcaga tcgtgcatcg cgtggtgcat acggtgtaaa
     4681 ccacaccaca gcggcagaac gatcatcagg aacaggaata cgcgaccaat gaagctctgc
     4741 gcgaacgcca gaacgcgctc gtagctcagc gcatcacccg gaaacagccc cagtggcagc
     4801 agaataccca ccagcaggat catcaccggc gcaataattg cgctccacat accaccggcc
     4861 ccgaagaggc cccagaatac cggttcgtca gaacgctttg gatttggatt aatcatctca
     4921 ggctccttac cagtacaggg caacaaacag gattacgatg gtggcaacca cagttaccgc
     4981 ccagagactt ttgataattg gctctggtcc cattttttcg tcttttacaa tgatattggc
     5041 cgctttcggt gccagttcaa accaggtttt ggtgtgcagc agagctgccg tcagagtgat
     5101 caggttaatg atcacgataa ccgggttttg taaaaagtcg acgaatcccg cccaggcttc
     5161 cgggccattt ttcagggcaa acagcccgaa aatcagttca atgctgaacc acacagccgg
     5221 aaccgccgtg ccttcgcgca gcatgtaaaa gcgataaaac ggcaattttt tccaccaggt
     5281 ggacgtcatt ggccgtacat acggtttacg tttagtcgtc atgttgcact ccttagcgtg
     5341 gtttcagggt cgcgataaga aagtctttcg aactttctac tttgccctgc tgaatggccg
     5401 cagccggatc gacgtgtttc gggcagactt cggagcaata gcccacgaaa gtacagctcc
     5461 atacaccgtt ctggctgttc aactgcgcca tacgctcctt cttaccgtgg tcgcggctat
     5521 cttcgttata acgatgcgcc agcgtaatgg cagccggacc gatgaactct gggttcaggc
     5581 caaactgcgg gcacgcggcg tagcacagac cacagttgat gcaaccggag aactggtgat
     5641 actttgccat ctgcgccggg gtctggatgt tagtaccctg atccgcggtg cgggagttgc
     5701 cgatgatgta cggtttgatc gcttccagac tttcgatgaa gtgggtcata tcgaccacca
     5761 gatcgcgttc aatcgggaag ttagctaacg cttcaacctt cataccgtcg gtgtaatcac
     5821 gcaggaaggt tttacatgcc agttttggca cgttgttaac catcatgccg caggaaccac
     5881 aaatcgccat acggcaggac cagcggtaac tcaggtccgg tgccaggttg tctttgatgt
     5941 agcccagcgc atccagtaat gaggtagttg cgtcataagg cacttcatag aatgcgctat
     6001 gcggtgcggt atcgacttcc gggttatagc gcaccacctc aattttcagg tttttcatct
     6061 cagccattcg ccttctcctt attattggct gcttccgcct tatcggctgc atccgcttcg
     6121 ccaccgtaaa cgcgtttagc tggcggcagc gtagtaatct tcacgtcgct gtactccagg
     6181 cgagtcgtgc catcagcatc gcggaaggcg agggtgtgtt tgagaaagtt gacgtcgtca
     6241 cgctcggtgc aaccttcgtc cagacgctgg tgtgcgccgc gggactcttt acgtgccatt
     6301 gcggagtgcg ccatacattc agcaacgttc agaccgtggc tcagttcaat ggtgtagagc
     6361 aggtcggtgt tgaacacgct ggaggtgtcg gtgatgcgca cgcgcttgaa gcgttcctgc
     6421 agctctgcca gcttgtcgat ggttttctgc atcagttccg gcgtacggta gataccgcaa
     6481 ccttcttcca ttgccaggcc catttcgtcg cggatcttcg cccagttttc gccgccatcc
     6541 tggttaacca gatctttcag acgttgttca acgccagctg cctgcgcttc aattgccgct
     6601 tcgttgccat taccggcagt tgctgcacgc tctgtcgctt gttcaccggc cagacggccg
     6661 aagaccacca gttccgccag ggagttggag cccagacggt ttgcaccgtg cagaccaaca
     6721 gaggaacatt cacccacggc gaacagacct ttaatgcggg tttcacagtt ctgatcggtt
     6781 tcgataccgc ccatggtgta gtgtgcagtc ggacgtaccg gaatcggttc tttaaccgga
     6841 tcgacgccaa cgtacgcttt cgccagttcg cagatgaacg gcagacgttc atgcagtttt
     6901 ttctcgccga ggtgacgcag gtcgagataa accacatcac cacgcggcgt ggagatggtg
     6961 ttgcctttac gccattcgtg ccagaaggcc tgagaaactt tgtcgcgtgg acccagttcc
     7021 atatatttgt ttttcggctc gcccagcgga gtttccgggc ccatgccgta atcttgcaga
     7081 taacggtagc catttttgtt gaccagaata ccgccttcac cacggcagcc ttcggtcatc
     7141 aggataccgg aacctggcag accggttggg tgatactgaa cgaattccat gtcacgcagc
     7201 ggaacgccgt ggcttagcgc catacccata ccgtcaccgg taacgatgcc gccgttggtg
     7261 ttgtaacgat aaacgcgacc cgcaccgccg gtagccataa cgaccgcgtt agcacggatc
     7321 tgcaccaacg tgccttccat catgttcatt gctaccaggc cgcgaacatg accatcatca
     7381 accagaatat ccagcacgaa atgttcgtca aaacgctgga tctgcgggaa ttgcagagag
     7441 gtctggaaca gcgtgtgcag catatggaag ccggtcttat cggcggcgaa ccaggtgcgc
     7501 tcgattttca tgccgccgaa gcgacgtacg ttgacgctac catccgggcg acggctccat
     7561 gggcatcccc acagttccag ttgggtcatt tcggttgggc agtggtggac gaaataatcc
     7621 acgacatcct gctcacacaa ccagtcgcca cccgctactg tatcgtgaaa gtgatattcg
     7681 aagctgtcat gatcctgcgc gacagcggcg gagccccctt ctgcagcaac ggtatggcta
     7741 cgcatcgggt atacttttga gattagtgcg atttttgcat ttggatttgc ctgcgcggca
     7801 gcaattgcag cacgtaatcc cgcgccaccg gcgcctacaa tggcaagatc ggcttgaaag
     7861 gtttgcacga cattcctcca gattgttttt atcccacagc cccgtacttc agggtaagtg
     7921 cctgaaagtt acggtctgcg aacgctattc cactgctcct ttataggtac aacagtatag
     7981 tctgatggat aagtctgaaa tttgacgaga tcgatttttt tagtgcgcaa gagggctaaa
     8041 ttatcactga agatgattaa tttaattact aaaccatcag atcgtgcttt ttttagtcac
     8101 tgcccaccgc tgtttgattc ctgcgtaaaa tttgtctcgc cgtcgcgttg cgagtagact
     8161 tcgtgccctt gtcaaaaact ggagatttaa ctatgagcga aacggcatcc tggcagccga
     8221 gcgcatccat tcctaactta ttaaaacgcg cggcgattat ggcggagatc cgtcgtttct
     8281 ttgccgatcg tggagtgctg gaggtggaga cgccttgtat gagccaggcg acggtaaccg
     8341 atattcattt ggtcccgttt gagacacgtt tcgttggccc cgggcattcg caggggatga
     8401 atctctggtt aatgaccagc ccggaatacc atatgaaacg cctgctggtt gctggttgtg
     8461 ggccggtatt ccagctgtgc cgcagcttcc gtaatgaaga gatggggcgt tatcacaacc
     8521 ctgagttcac tatgctggag tggtatcgac cgcactatga tatgtaccgg ttgatgaacg
     8581 aggtggacga tctcttacaa caggtgctgg actgcccggc agcagaaagc ctttcttatc
     8641 aacaagcttt cttgcgttat ctggaaattg acccgctctc tgccgacaaa acgcaactgc
     8701 gggaagtcgc agcgaaactg gatttgagca atgttgctga taccgaagaa gaccgcgaca
     8761 cgttgctaca attgctgttt acctttggcg tagagccaaa tattggtaaa gaaaaaccga
     8821 cctttgtgta ccactttcca gccagccagg catcactggc gcaaatcagt accgaagatc
     8881 atcgggtcgc tgaacgcttt gaggtttatt ataaaggtat tgagctggcg aatggtttcc
     8941 atgaattaac ggatgcccgt gagcagcaac aacgctttga acaagataac cgtaagcgcg
     9001 cggcgcgcgg tttgccgcag caccccattg accagaatct gattgaagcc ttgaaagtcg
     9061 gtatgcctga ctgctccggc gtggcattag gtgttgatcg tctggtgatg ttggcgctgg
     9121 gcgcggagac actggctgaa gtcatcgcct ttagcgttga ccgggcataa ttctgaaaaa
     9181 ttactgaatt aacagcgaag aatggcgtga taagcgccat tctgtagcat atgttttcat
     9241 ttttccgtta aaggccacaa tttaagactg gatatttgat atcatcaagg tatcaattct
     9301 gtattggttt ttacgctgcc tgctcaatgt tgcgcagtgc agttcagtgg gcacgtgtta
     9361 tacacgcgct gaaatgaagg atggtttcat gcctcacacg ataaaaaaga tgagtctgat
     9421 aggactcata ttgatgatct ttacttccgt atttggattt gccaatagcc catcggctta
     9481 ttacttaatg ggttatagtg cgattccctt ttatatattt tctgcattgt tattctttat
     9541 tccattcgcc ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat
     9601 ctattcctgg atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg
     9661 gttttcctct tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc
     9721 aacattcctc tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac
     9781 gcaggtggtt ggtctgctgg cagtagcatg gatgattctg gtcaccgtcg ttgcttcaaa
     9841 ggggattaat aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa
     9901 tttagtattg ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca
     9961 ggatattaat ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc
    10021 gtttgtggta tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga
    10081 taaaacggaa aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat
    10141 ttcaatcggt tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt
    10201 attaagtaat ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg
    10261 ggtgacgctg ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg
    10321 gtttgcgcgt attaccggac tttcgatgtt cctcgcttat accggtgcgt tctttacgct
    10381 ttgctattca ccgctgaaag ccatcatcca ggggacgccg aaagcgttgt ggccggaacc
    10441 gatgacgcgc ctgaatgcga tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt
    10501 ggttactgtc ttcattctgc tggtttcgtt tggtggcggt accgcatcgg cgttctttaa
    10561 caagctgacg ctgatggcga acgtgtctat gacgcttcct tacctgttcc tcgcgctggc
    10621 tttcccgttt tttaaagcac gtcaggatct cgacagacca tttgtgattt tcaaaacgcg
    10681 tatgtcggca atgattgcga cggtggttgt cgttctggtg gtgacatttg cgaacgtctt
    10741 caccattatt cagcctgtgg ttgaagccgg agactgggac agcacattgt ggatgattgg
    10801 cggccctgtc ttcttctcgc tgttagcgat ggcgatttac cagaactatt gcagtcgcat
    10861 ggcgaataaa cctgagttag ctctcgactg atcatatgaa atcctgcaac tcatcgtctc
    10921 tccggcgatg agttgtcagg aagtaatgcg tatggatgat actctccagg gccggtgata
    10981 actgaagatg atgttaagct gctaaatgag ccacgttgac atcatttggc agcttcatgc
    11041 attacaaact tcccgcctga cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt
    11101 taccgttgag gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg
    11161 cgtggaagcc tgccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca
    11221 tctcagcggc gtaaatacgc agctcgaaaa tctgaatccc ctgttgcaga tccaccagga
    11281 agacttccgg tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga
    11341 tttccgtcac ttcttcactg ttggcatcgg caggggccgg aatcgtcaac actacgcgcg
    11401 tgaccgagtc agagagcgac cagttgataa actgctcggt aataaacgcc ttgttcggca
    11461 cgattatctc tttacggtcc cagtcgctga tggtggtggc gcgggtgtta atttttgtca
    11521 cgctaccagt aagatcgcgg atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca
    11581 ggatgatcag gccagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa
    11641 caccgagcgc ggcaaccagc cactgcaatt tcgaccactc aataccaatc attgagaagc
    11701 cgaccagccc gccaatcagc atcagcagat attttgtgat ggtggtgatg gcgtaacccg
    11761 tacccggcgt taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc
    11821 gcaccagctg cgtggtgatg ataaatacca gaatggcaat cagcaccgca ccgagggtaa
    11881 ttggctccag actttctacg ccctgtaccg tggaggtgac atcccacagc gaaatatttt
    11941 cgaggaagcc gaaagcggaa tggatttctg accacagcac aatgaccgaa agcagggcga
    12001 tcaacatcaa aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga
    12061 cttcgctttc atcgacttca attgctcctt ccgggctact gtgatgatgc gcttcctctt
    12121 cgccacgcgc acgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca
    12181 gcctgcgacg ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga
    12241 accagatggc aatcgaggtt tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac
    12301 ccaccgccga cgccagaatg gcaaccagtg gtgcgccaat catcatgttc cacagcatat
    12361 ggttggtaat gttgtcgccg ctgccttctt tgttgagata caacggaata ccggcttttt
    12421 tcaggcttag ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccgtccca
    12481 gcgaaccgga aaattcgcgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc
    12541 cgatgctcat caggtagtag cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat
    12601 gagcgataaa caagccgttc gggcgggcaa aggtggcgca aatcatcact acccacagca
    12661 gcggcacggt ggccgttaca ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct
    12721 cgcgcaagcc gtaacccagc gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg
    12781 accagaaaag agtgcgcaac gtcagccaga agtgatcctg agtcactttg ccgactttcg
    12841 ccgccgaacg ttcaagaaaa cgggtgaaat agcggcgcga gtaaatactg caaccgacca
    12901 gaatcaacgc gccaaacagc ggcaaaattg tctctttgct ggtcagcatc atcacactgg
    12961 ctttgcccaa ctgactgaag gtgtccagcg aaatgagacg acgcagatcc tgggcgattt
    13021 ccagcggcca ggcgatggtc atcgggcgca cgtcagaggt ccagaacaga tagcggtgcg
    13081 ttgcttcgtt cacctctttc agcgcatcct ccagttgccc gttggagact ttcagcttgg
    13141 tcagttccaa tagtagcgtg tcgccaccct gcaacaatga gttcagcaac tcacgctgag
    13201 tgcgcagttg tgcttccaga atacggtttt gctcggcggt cagcggctga ccgtcggcct
    13261 gatggatttg ccgtagcagc ggctgtttat taagtaaatc ctcataacgt aaccgttgca
    13321 cacgcaactg cgccatttcg gtatcaagct gttgtggttt tggcatttct ggtagccgtg
    13381 ccacctgcgc ccgcagcgct tcgccgagca ggttggacga tcccagccat tgcgactgtt
    13441 cacgcagcgt attcaacgcc tgccggacct gtaacgtctg gctggcagcc tgacgctgtt
    13501 gcgaggcaac gagctccatc cgctgcgcct gttgattcag cgctgccgat agttcgcggt
    13561 taattttgaa ttgcgcgacg atatctttcg gcaaatcggc actgttttct gccagtaatt
    13621 cggtactttc cagcgcccgc tccgcctcaa gctgacgttg gctattcaac tgattacgta
    13681 agccctgcaa atacgcatcc agttgctggc tctctttttc cgccagctct gagcgtaagc
    13741 gcgctaattc ctggcggtta ttggcagaca gctgcgccag ctccagttca tcaacgagcg
    13801 ccttaagacg tgcagagtca gactgcaacg cgaaattttg tgcctgattg agcggagtat
    13861 tgccggtaag cgttcccagg cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt
    13921 gctgttgcgg cagttgattc agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct
    13981 gggcctgacg gcttttatcc agcaactggc tgctgacctg gagaatttcc tgattcagcg
    14041 cgtcggtaga cattcccggc gacacgctgc gcggctcgtc acgcatgttg tttaattgtg
    14101 cgcgcagagt agcggagagt ttcggataat tatcgataac ttgctgatat tgtttgatgc
    14161 gctcaaggga accttttcgt tcctcaagcg catttaaggc agactggagc gcctctacga
    14221 cttccggctg tgcgggtttc gccgcttttg cctgctccag ttcctgagtg atttgtttgc
    14281 tatcgggggc cgtcgcggcg tacgcccccc aactgaggca ccaggccatc agaaaagtga
    14341 taatcaggcg cacgtcagcg tttcctttga tggattagac ctggtctttt ttatcgtcaa
    14401 ccaatggcct ggcgtcgtgt tctgcttcga tctcttcagc aggaagtggg gcgggttcag
    14461 cgtctggcgt aacaaaggtt tcggtagata ctgccagcgg ctggccaatt ttcgtgacag
    14521 acaggctttc cagttgctca accagattca ctttacccgg tgcaaacagg ttgataacgg
    14581 tggaaccgag tttaaagcga cccatttcct ggcctttcag cagtgccaca gaaccgtcgt
    14641 tttccccggc aggccaggtc cagcgcttga tgataccttc gcgcggcggc gtaatggtgc
    14701 ccgcccagac cgtctcaatg ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg
    14761 ggccaaattc ggtatcgaaa aggcaaatca cgcgttcgtt acgggcaaac agattcggca
    14821 cgttctgagc ggtgagatgg ttaacggaaa agagatcgcc cggcacgtag atcatctcac
    14881 gcagaatacc gttgcacggc atgtgtacgc gatggtagtc acgcggggag aggtaagtgg
    14941 tcacaaacgt accgttgcgg aacaggtccg ccatcagata gttgcctgcc agcagggctt
    15001 cgaggctgta gttgtggcct ttggcttgca ggattttatc ttcttcgatt ttacccaact
    15061 ggctgataac gccatcggca ggcatgacca gtacattcgg atcggtatcg attgggcgta
    15121 cttcgtcacg cagcggacgg acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg
    15181 gcttttgcgc ctctttcatg tcgaccttgt agtatttaac gaacagatcg ataaccagtt
    15241 ttgtcagcca tcctgcccgc ttgcttgcgc cccaacccgc caggcgagta agccatagtt
    15301 tcggcagaat gtactgtagc gaaagtttaa atgaatttaa caaggtagcc tccaggccat
    15361 tgttttgtcg ttcctgatcc ggccaacatg ccggatcctg aaaaaaaggg gacgattcta
    15421 acgacggtta gcttaattgt cagccatccg tatcagaaaa gtttttacgc gtttttacct
    15481 gcgccatgct ttccagaata cggtgatagt tttcgaaacg ggtttccgcg attttccctt
    15541 cctcaaccgc ttcccggata gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt
    15601 tacacagacc taaatagtca tggaattcga caaagccctg agtgatttgt tccggctcca
    15661 ggtgccagag gccgaactca cgcacaccag gggaatcaat cacatcaccg ccgtgcggga
    15721 agtgatacag ccgagcggcg gtcgtggtgt gctgaccgag ccccgagttg tcagagacat
    15781 cgttggtcag gatctctttt tgcagcccca gcaacgcgtt cagcaggctg gatttaccga
    15841 cgccagactg cccggcaaaa atgctgatgc gcccggtcaa cgcctcttcc agcggtttca
    15901 gcccatcctg agtatggctg gaaaccatca atacgcgata accgatgttg cggtagatat
    15961 ccatctgctc gttgacgaac gccatgcctt cgtcgtccag caggtctatc ttgttgagca
    16021 cgataatcgg ctcaatctgc aaggtttcgc aggccaccag gtaacggtcg ataatattga
    16081 gcgacagctc cggcaaaatg gcggagacaa tgacaatctg gtcgatgttg gcggctatgg
    16141 gtttcacgcc gtcgtagaaa tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt
    16201 ccacgatccc tttgatattt acgccttccg ccgccggttt acccggacgc cagactacgc
    16261 ggtcgccggt taccagcgaa cggatggtac ggcgaatatt gcagcggtga acgtcgccat
    16321 cggcggattc cacatcagcg tgcataccaa agcggctgat gacgatacct tcatcaggct
    16381 cgccaaacag attgtcgtcg tagtcgggct tctccttaga cgttttaaga cgacgctggt
    16441 gattggcgtt cacgcggcgc tgctggcctt tggagagttt atttttactc aatcgtactg
    16501 actcctggtc gcccctgatg ggcaaaacat ctatgataca cgcaattgtg gatcaatata
    16561 gtcactgtga atgggtggaa aatagcatga gtgccaatga aaacaacctg atttggatcg
    16621 atcttgagat gaccggtctg gatcccgagc gcgatcgcat tattgagatt gccacgctgg
    16681 tgaccgatgc caatctgaat attctggcag aagggccgac cattgcagta catcagtccg
    16741 atgaacagct ggcgctgatg gatgactgga acgtgcgcac ccataccgcc agcgggctgg
    16801 tagagcgcgt gaaagcgagc acgatgggcg atcgggaagc tgaactggca acgctcgaat
    16861 tcttaaaaca gtgggtgcct gcgggaaaat cgccgatttg cggtaacagc atcggacagg
    16921 accgtcgttt cctgtttaaa tacatgccgg agctggaagt ctacttccac taccgttatc
    16981 tcgatgtcag caccctgaaa gagctggcgc gccgctggaa gccggaaatt ctggatggtt
    17041 ttaccaagca ggggacgcat caggcgatgg atgatatccg tgaatcggtg gcggagctgg
    17101 cttactaccg cgagcatttt atcaagctgt aaaatttttc ctggtcacgt aagcgccgga
    17161 atccacttgc ggcggcgtga ttttgacgct aaatcgaacg tcttgctgat aatttcagca
    17221 cttgagataa aaacgcaaaa aaaacttttt tggggggttg cagagggaaa gatttctcgt
    17281 ataatgcgcc tcccgtaacg acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt
    17341 atgcgggaat agctcagttg gtagagcacg accttgccaa ggtcggggtc gcgagttcga
    17401 gtctcgtttc ccgctccaaa atttgaaaag tgctgcaaag cacagaccac ccaatcgcag
    17461 tatttcgaat tgcgacaagg cggcaactga acgaatcctt gggaacttac aaaagtatgt
    17521 gactaaggtg agtgaaggca gccaaggcag tagcggttcg aaagacgaag at
//
LOCUS       DAJTXY010000078        17448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTXY010000078 DAJTXY010000000
VERSION     DAJTXY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.078"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            564..1334
                     /locus_tag="OO566_002711"
     CDS             564..1334
                     /locus_tag="OO566_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6605935.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO566_002712"
     CDS             1325..1798
                     /locus_tag="OO566_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6605936.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO566_002713"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO566_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6605937.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPVSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO566_002714"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO566_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6605938.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO566_002715"
     CDS             3233..3727
                     /locus_tag="OO566_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6605939.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="OO566_002716"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="OO566_002716"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6605940.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO566_002717"
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="OO566_002717"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6605941.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8485
                     /locus_tag="OO566_002718"
     CDS             7571..8485
                     /locus_tag="OO566_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6605942.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGILTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8699..10060
                     /locus_tag="OO566_002719"
     CDS             8699..10060
                     /locus_tag="OO566_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6605943.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="OO566_002720"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="OO566_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6605944.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="OO566_002721"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="OO566_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6605945.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="OO566_002722"
     CDS             12610..13899
                     /locus_tag="OO566_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6605946.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPQVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="OO566_002723"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="OO566_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6605947.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVDDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLHQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="OO566_002724"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="OO566_002724"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6605948.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="OO566_002725"
     CDS             16225..16605
                     /locus_tag="OO566_002725"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6605949.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17448
                     /gene="hpaH"
                     /locus_tag="OO566_002726"
     CDS             16673..>17448
                     /gene="hpaH"
                     /locus_tag="OO566_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6605950.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGHPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGN"
BASE COUNT         4293 a         4624 c         4371 g         4160 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccggtaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcat
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agttcggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg caggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtggacg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcac cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgagct gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcat ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacat
//
LOCUS       DAJTXY010000079        17397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTXY010000079 DAJTXY010000000
VERSION     DAJTXY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.079"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="OO566_002727"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="OO566_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6605951.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3172)
                     /gene="hemX"
                     /locus_tag="OO566_002728"
     CDS             complement(1967..3172)
                     /gene="hemX"
                     /locus_tag="OO566_002728"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6605952.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3194..3934)
                     /gene="hemD"
                     /locus_tag="OO566_002729"
     CDS             complement(3194..3934)
                     /gene="hemD"
                     /locus_tag="OO566_002729"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6605953.1"
                     /translation="MSILVTRPSPAGEELVRRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3931..4872)
                     /gene="hemC"
                     /locus_tag="OO566_002730"
     CDS             complement(3931..4872)
                     /gene="hemC"
                     /locus_tag="OO566_002730"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6605954.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5259..7805
                     /gene="cyaA"
                     /locus_tag="OO566_002731"
     CDS             5259..7805
                     /gene="cyaA"
                     /locus_tag="OO566_002731"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6605955.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7845..8165)
                     /gene="cyaY"
                     /locus_tag="OO566_002732"
     CDS             complement(7845..8165)
                     /gene="cyaY"
                     /locus_tag="OO566_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6605956.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8213..9526)
                     /locus_tag="OO566_002733"
     CDS             complement(8213..9526)
                     /locus_tag="OO566_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6605957.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9523..11001)
                     /locus_tag="OO566_002734"
                     /pseudo
     CDS             complement(9523..11001)
                     /locus_tag="OO566_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            11190..11393
                     /locus_tag="OO566_002735"
     CDS             11190..11393
                     /locus_tag="OO566_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6605958.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11430..12254
                     /gene="dapF"
                     /locus_tag="OO566_002736"
     CDS             11430..12254
                     /gene="dapF"
                     /locus_tag="OO566_002736"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6605959.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12251..12958
                     /locus_tag="OO566_002737"
     CDS             12251..12958
                     /locus_tag="OO566_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6605960.1"
                     /translation="MKQLGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12955..13851
                     /gene="xerC"
                     /locus_tag="OO566_002738"
     CDS             12955..13851
                     /gene="xerC"
                     /locus_tag="OO566_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6605961.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13851..14567
                     /gene="yigB"
                     /locus_tag="OO566_002739"
     CDS             13851..14567
                     /gene="yigB"
                     /locus_tag="OO566_002739"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6605962.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14651..16813
                     /gene="uvrD"
                     /locus_tag="OO566_002740"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14651..16813
                     /gene="uvrD"
                     /locus_tag="OO566_002740"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6605963.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16960..17352)
                     /locus_tag="OO566_002741"
     CDS             complement(16960..17352)
                     /locus_tag="OO566_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6605964.1"
                     /translation="MRLGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGAMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         4058 a         4481 c         4604 g         4254 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatctacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtttagc ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct
     2101 gcatcagttt ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat
     2161 tttgctggct taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat
     2221 cgtagtaagc acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct
     2281 gatgacgcgg tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat
     2341 agatatcctg atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa
     2401 tgaagttgtc cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg
     2461 aactggaaag ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca
     2521 gacgcaggtt atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat
     2581 ccacctgcga tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa
     2641 tcagactcgg gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag
     2701 aggtcgtgac gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg
     2761 cctgagccag cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga
     2821 cttcatccaa ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag
     2881 cttgttgctt aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg
     2941 ccgtcagttg gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac
     3001 cccagccata caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa
     3061 tcaatgcagt attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca
     3121 cggcctccct ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt
     3181 cctgttatga gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga
     3241 ccttaatgtc ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca
     3301 atagtcgaca gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt
     3361 gctgcaacat ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca
     3421 tcgcttcttc tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga
     3481 cctcagcacc gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta
     3541 atatcagcgc acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc
     3601 tgatttcccg atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg
     3661 cggtggtgcg tccaatggtg aaataagtag gtagttgggg ccatttacga tcttgctgat
     3721 gcagctgtga ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct
     3781 cccccagcgc tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa
     3841 tcagcggaaa atgccaggcc acctgcccca gtgtgcgcag acgtctcact aactcttctc
     3901 cagcgggaga cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc
     3961 agcgaggatc tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg
     4021 ttcggcatct tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc
     4081 gccgaccagc gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg
     4141 cacctgacat ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc
     4201 agtttcgtgg tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca
     4261 ttcaataccg actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc
     4321 gcgaatgcgg gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc
     4381 gtattcgccg ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat
     4441 aatcagatcc gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac
     4501 gatactgcct gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg
     4561 aggatcttca cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac
     4621 atctttcatt gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc
     4681 ttttacaaat aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc
     4741 gcgcgtcacc atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc
     4801 tttgacatag tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac
     4861 attgtctaac atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca
     4921 gagtgatgtc agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg
     4981 gcagtggatc ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg
     5041 gtgctacact tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga
     5101 tcacgtttta gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa
     5161 cggttacctt tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga
     5221 atcacagtca tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc
     5281 tgaaacagag actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg
     5341 ggcctgcatt ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc
     5401 taatgccggg ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg
     5461 aaactcaacg ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc
     5521 caccgaaagg tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag
     5581 ggcaaagttg ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg
     5641 aagagcgcca gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg
     5701 gtgtggaagt cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca
     5761 gcctgggggg cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc
     5821 gtactgccgt gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag
     5881 aagagcatta cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg
     5941 aatggctgga tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc
     6001 tttggcagct ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc
     6061 tggaagccta ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc
     6121 gtttgcacga cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc
     6181 gtgttactga atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct
     6241 gcttctattt aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc
     6301 gcgcagtgtt gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc
     6361 tcgataaccg tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg
     6421 acgcgatgat gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg
     6481 tctccgccag tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag
     6541 cattgccagg taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga
     6601 atctgacctt tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata
     6661 accgcgcgcc aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc
     6721 tgaataaact ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt
     6781 atattaaagg taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt
     6841 cgcaccattt cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg
     6901 agattcgcca tctggcgatt atcgttaacc tggaatatga cccgacagct gcgttccgca
     6961 atcaggtggt gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa
     7021 attgcctggt aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc
     7081 tgcacttcaa cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc
     7141 atcaggacgc cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg
     7201 gcttaatccg tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca
     7261 gcacccgcca ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct
     7321 tgttcttcga acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg
     7381 gcgctatttc gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca
     7441 aattaccggc ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag
     7501 aaacgcaaga cgagaatggc tttaatatct acattctcga cgaaagcaac cgggttgagg
     7561 tgtatcacca ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact
     7621 cgtcatcgca cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt
     7681 tctatcagat tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga
     7741 ttggcaacct gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt
     7801 cgtgatgagc gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact
     7861 gtttcacccg cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg
     7921 cgatcgcaaa tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc
     7981 agccacacct ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag
     8041 gtaatggtca gtacgccgcc gttgatttcg cagtcgatat cgctgtcgcc atccctgtcg
     8101 tccagacgct cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg
     8161 ttcattgttg taccctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct
     8221 tcaaagtttg cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga
     8281 tgtctctgtt ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg
     8341 gggagggcgt aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag
     8401 cgaagcgatc gtttttagtt agcggaccgt taactggaat aatttgcaag ctctttgacc
     8461 gtaattggcg gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca
     8521 agtctggcgt atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg
     8581 ttacatacag ccgatcttta tattgcgcaa tattgccagt aatcagatgg aattgttcag
     8641 gttcaatacc tggtaggtct atccacttgc tccactgacg gtcgggcgtg agctgtttac
     8701 gccagcgaac gcgactttcc cctacttcaa aagagacgtc atcatctacg ggtatcgctt
     8761 tgcctgataa ttgaccaaag ggataacgat gcagaccctt attgtcgcga aaaaccagca
     8821 gtgaatgagg tatccagcga attatctcaa atgagtctgc attcgccgta aggtgaacgc
     8881 catttaaata aatattctgc ccactgcgga taagcagatc attggaatat gaatttctgt
     8941 ttacactcat aagacgtgga ttaatacccc accatctttt ttctaataca gtaacatcac
     9001 tgctgctccc ctgacgtcga ccggacaagt agagattttt actatccacc agcgtggtag
     9061 agttaccttc gatagctttt agtgtcgcca gatcaacacg tgacgcgccg ctattacttt
     9121 cggtgcgctg tccgtcaaaa tagaggctat atttatcgac cgcgaagcgg cccatagcct
     9181 gaaagctggc aatatcgaca gtgggtgttc ccggtggatt gctaacgatt ttgccacgcc
     9241 agagaataac gcggccgtct gtcacccagt ggtattcctc attttctatg cttatttcat
     9301 cggaaggtat ataacccgtt ttcccagaga tatttttcac aaggcgcaaa ccggccctgc
     9361 tcatctccgg aaactgggca atgattttct tatcccccca ataaccttta ccgtaagaca
     9421 taacaccgtg atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg
     9481 tgtgtcccaa caggatgagt gcaactgtga ttatttgacc cttcattttg tcacctcatt
     9541 gcgcggggca ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt
     9601 gtaatttacg ccatagacat atcccgaatg ttgttccttc cagattaagc gaacttgttt
     9661 cggatcagcg gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt
     9721 atcgcgggca taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc
     9781 cggagaacgg ttatccagta agatcagcgg tccgtcagat tcaaacacgt gtaaaccgga
     9841 atggtatggc tacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa
     9901 acgtttgttg ataataagat tgggatcatc aagtttcacg atctccagct tacggtcacc
     9961 agactctgtt tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat
    10021 gggttgaaac tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt
    10081 acgccagaac actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc
    10141 atcccacgcc agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca
    10201 ggtcaagagt gagccaggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag
    10261 ctgttcgccg ttgagcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa
    10321 tggtacggca atgcaggggt taaacgtggt ggaaaacttt cctcgctgat cccaggactt
    10381 ctgcgccagc acacggaaac tctcggggtc agcgaggcga tgcccattga tatacaagaa
    10441 attagcgtca cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac
    10501 ctgatgcagc gttttgatat ccaagctatt tccaccacca ttgtcatcgg tgcgcttgcc
    10561 ttcaaaatag agactgtgtt tatctgcggc aaaatcaccc catgccttga acgaggcgac
    10621 atcgacggga ggtgtgccgg gagggttttg cacaacttca cccgcgtaga ggatattgcg
    10681 accatcgcta tgccatgaat aatgaggata ctaggcctgt gcgccctcat agaggtagtc
    10741 ggttatcaaa tcagtcagag gcggttcttt atcccattcg tctagtgttg tcgggtctat
    10801 taataaattg ggtaaccgac gcaaactctt gtaattcacg tcaggtagag aaagatttgt
    10861 ttctttataa gggcgcaggt acaccacgcc ttttccctgt ttctggtaaa tattatgttc
    10921 aggtgcggaa agccgacatc cccatgccga cgataaaaac aatgaacaag ccaacaaaca
    10981 aaccacattg cgatagtgca taaagccatc ctggcgcgag gtgttgatca cgaaactgcc
    11041 agcagaacat aaatcctcac gagtaagcgt tatactcgca gcatttcctc acttttcaga
    11101 cttcataaag agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag
    11161 aaagcgatga actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat
    11221 tacttactct cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg
    11281 cagataaaaa cgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc
    11341 cggataaaaa cgatcgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag
    11401 tttctgtacc cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca
    11461 acgattttat ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc
    11521 gtcgcctggc ggatcggcac ctgggggtag ggtttgacca gctgctggtg gttgagccgc
    11581 cgtatgatcc tgaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg
    11641 cgcagtgcgg taacggtgcg cgctgctttg cccgttttgt gcgtctgaaa ggactgacca
    11701 ataagcgtga tatccgcgtc agcaccgcca acgggcggat ggttctgacc gtcaccgatg
    11761 atgatctggt ccgcgtaaat atgggcgaac ccaacttcga accttccgcc gtgccgtttc
    11821 gcgctaacaa agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg
    11881 gcgtggtgtc gatgggaaat ccgcattgcg tgattcaggt cgatgatgtc gataccgcgg
    11941 cggtagaaac gcttggtcct gttctggaaa gtcacgagcg ttttccggag cgcgccaata
    12001 tcggttttat gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg
    12061 caggagaaac ccaggcctgc ggcagcggcg cgtgtgcggc ggtggcggta gggattcagc
    12121 aaggtttgct ggacgaagaa gtacgcgtgg aactccccgg cggtcgtctt gatatcgcct
    12181 ggaaaggtcc gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat
    12241 ttattcatct atgaagcaac taggggaaga actgcaggaa acactcacgg agcttgatga
    12301 ccgggcggtt gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc
    12361 agtagaagcg atacgtgtgc cgcatccggt acgcggcacc gtttcgttgg tcgagtggca
    12421 catggcccgc gcacgtaatc acattcatgt tctggaagag aacatggcgt tgttgatgga
    12481 gcaggctatc gccaacgaag ggctgtttta tcgcctactc tacctgcagc gcagtctcac
    12541 cgccgccagc agtctcgacg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg
    12601 cctggctggt gcgagtctgc gcctgtttcc agatcgctgg cgcttaggtg cgccgtcgaa
    12661 ccacactcat ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtttggg
    12721 gcaggaacag cactatcttg ggccgcttaa cggaccggag ctgctggtgg tgctaccgga
    12781 agcgaaagcg gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggtgt
    12841 tgtgctgttt accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact
    12901 tcatgaaatt gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc
    12961 gatttacaca ccgatgtaga acgctaccta cgttatctga gcgtggagcg tcagcttagc
    13021 ccgataaccc tacttaacta ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa
    13081 aacggcctgc aaagctggca acaatgcgat gcagcgatgg tacgcaattt tgctgtacgc
    13141 agtcgccgta aagggctggg agcagcaagt ctggcgttac ggctttctgc gctacgtagc
    13201 ttttttgact ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggagtttcg
    13261 gcaccgaaag cgccgcgtca tctgccgaaa aatatcgacg tcgacgatat gaatcggctg
    13321 ctggatattg atatcaacga tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg
    13381 tacggcgcgg gtctgcgtct ttccgagctg gtggggctgg atatcaaaca cctcgacctg
    13441 gagtccggcg aagtgtgggt gatggggaaa ggcagcaaag agcgccgcct gccgattggt
    13501 cgcaacgctg tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa
    13561 gacgacgcgc tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa
    13621 cgctttgccg aatggggcat aaaacaaggg ctgaataatc acgttcaccc acataaatta
    13681 cgtcactcgt tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag
    13741 ctactgggtc atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac
    13801 cttgcctcgg tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt
    13861 accggccttt ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata
    13921 accgtccggt gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc
    13981 cggcgctgcg cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag
    14041 cggaacccga gatttatcac gacgtgacgc gttggcgttt tcgttcgatt gaacaagcga
    14101 tgctcgacgc cgggctgagt gccgaagaag ccagtgcagg cgcacacgca gcaatgatta
    14161 actttgccaa atggcgcagc cgaatcgacg tcccgcagca aactcacgac accttaaaac
    14221 agctggcgaa gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt
    14281 ttggtttggg ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac
    14341 cgttcagcga tatgtacttt ttggctgcgg aaaaactcaa cgtgccgatc ggcgagatct
    14401 tacatgttgg ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg
    14461 cttgttggat cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc
    14521 cacatctgga aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt
    14581 atatataccc agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc
    14641 ggcggtgcca atggacgttt cttacctgct cgacagcctt aatgacaaac aacgcgaagc
    14701 ggtggccgcg ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg
    14761 cgtactggtg catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat
    14821 tatggctgtg acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcggacaact
    14881 gatgggcacc agccagggcg gcatgtgggt aggcaccttc cacgggctgg cgcaccgcct
    14941 gctgcgtgcg caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga
    15001 agaccagcta cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg
    15061 gccgccgcgc caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca
    15121 tcatattcaa agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta
    15181 tcaggaagcg tgcgatcgcg cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca
    15241 cgaactgtgg cttaacaagc cgcatatcct acaacactac cgcgaacgtt ttaccaatat
    15301 cctggtggac gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc
    15361 gggcgacacc ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg
    15421 cggggcgcag gtggagaata ttcagcgttt ccttaatgat ttccccggtg ccgaaattat
    15481 tcgtctggag caaaactacc gctctaccag caatattctg agcgccgcta acgccctgat
    15541 tgaaaacaat aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc
    15601 tatttccctc tattgcgctt ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat
    15661 caaaacctgg caggacaacg gcggggcgct tgccgagtgc gccattctct accgcagtaa
    15721 cgcccagtcg cgtgtgctgg aagaagcact gttgcaggcg agtatgccgt accgtattta
    15781 cggcgggatg cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct
    15841 gattgccaac cgtaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg
    15901 tattggtgac cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact
    15961 ctggcaggca tgtagtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc
    16021 cttacagcgg tttatggaac tgatcgacgc cttagcgcag gaaactgccg atatgccgct
    16081 gcatgtacag actgaccggg taattaaaga ctccggcctg cgtaccatgt acgagcagga
    16141 gaagggcgaa aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg
    16201 ccagttcagc tacaacgaag aagacgaaga tttaatgccg ctgcaggcgt tcctatccca
    16261 tgcggcgctg gaagcaggcg aagggcaggc ggatacctgg caggatgcgg tacaactgat
    16321 gaccatgcac tcggcgaaag gtctggagtt cccgcaggtg tttatcgtcg gtatggaaga
    16381 gggcatgttc ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg
    16441 tctggcctac gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac
    16501 tcgccgtctg tatggcaaag aggtttacca tcgcccgtcg cgctttattg gcgagttgcc
    16561 ggaagagtgt gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca
    16621 gcggatgggt acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgtgtacg
    16681 ccacgctaag tttggtgaag gcaccatcgt caatatggaa ggcagcggtg aacatagccg
    16741 tttgcaggtg gcattccagg gccagggaat caaatggctg gtggcagctt acgcccggct
    16801 ggagtcggtg taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat
    16861 tcagattcaa taaattgcaa cgtcatgtag gcaggataag gcgtttacgc cgcatccggc
    16921 atcagcagca tatttagtat ctgacacaaa gctatcattc taacctttcc gctcgacgga
    16981 aatcatggtg acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg
    17041 tgaaatcgtg ccaccaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca
    17101 ggcaaaatca taaaaatttg tcgcctgctg gctcaacaaa aacacggcat tcccatgttt
    17161 attaatccca acaccgttac gaattttacg tgaggcgacg ttgggatgaa tacgcggatt
    17221 aattacaccg ttttccatca acattgctcc tgactgcacc gcaaactgaa tatctttact
    17281 ggctttaaaa gcatccagac gaacgatgcc gactttatct cccgcgacat aaaacacgcc
    17341 gccaagacgg ataaagaaat tcccttcacc tgaagcgaga tttaacgcta ccttctg
//
LOCUS       DAJTXY010000080        16770 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTXY010000080 DAJTXY010000000
VERSION     DAJTXY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.080"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(149..733)
                     /gene="kptA"
                     /locus_tag="OO566_002742"
     CDS             complement(149..733)
                     /gene="kptA"
                     /locus_tag="OO566_002742"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6605965.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            982..1665
                     /locus_tag="OO566_002743"
     CDS             982..1665
                     /locus_tag="OO566_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR6605966.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1662..2123
                     /locus_tag="OO566_002744"
     CDS             1662..2123
                     /locus_tag="OO566_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6605967.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2136..3308
                     /gene="iadA"
                     /locus_tag="OO566_002745"
     CDS             2136..3308
                     /gene="iadA"
                     /locus_tag="OO566_002745"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6605968.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTLFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3373..4284
                     /gene="hypT"
                     /locus_tag="OO566_002746"
                     /gene_synonym="qseD"
     CDS             3373..4284
                     /gene="hypT"
                     /locus_tag="OO566_002746"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6605969.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4277..4669)
                     /gene="iraD"
                     /locus_tag="OO566_002747"
     CDS             complement(4277..4669)
                     /gene="iraD"
                     /locus_tag="OO566_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6605970.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4666..4749)
                     /gene="idlP"
                     /locus_tag="OO566_002748"
     CDS             complement(4666..4749)
                     /gene="idlP"
                     /locus_tag="OO566_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6605971.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5342..6172
                     /locus_tag="OO566_002749"
     CDS             5342..6172
                     /locus_tag="OO566_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6605972.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(6313..7086)
                     /gene="uxuR"
                     /locus_tag="OO566_002750"
     CDS             complement(6313..7086)
                     /gene="uxuR"
                     /locus_tag="OO566_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6605973.1"
                     /translation="MKSVTSVQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7301..8761)
                     /locus_tag="OO566_002751"
     CDS             complement(7301..8761)
                     /locus_tag="OO566_002751"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6605974.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLTLGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8842..10026)
                     /gene="uxuA"
                     /locus_tag="OO566_002752"
     CDS             complement(8842..10026)
                     /gene="uxuA"
                     /locus_tag="OO566_002752"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6605975.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10366..11709
                     /gene="gntP"
                     /locus_tag="OO566_002753"
     CDS             10366..11709
                     /gene="gntP"
                     /locus_tag="OO566_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6605976.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11960..12862)
                     /gene="fimH"
                     /locus_tag="OO566_002754"
     CDS             complement(11960..12862)
                     /gene="fimH"
                     /locus_tag="OO566_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6605977.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12882..13385)
                     /gene="fimG"
                     /locus_tag="OO566_002755"
     CDS             complement(12882..13385)
                     /gene="fimG"
                     /locus_tag="OO566_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6605978.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13398..13917)
                     /gene="fimF"
                     /locus_tag="OO566_002756"
                     /pseudo
     CDS             complement(13398..13917)
                     /gene="fimF"
                     /locus_tag="OO566_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
     gene            complement(13927..16563)
                     /gene="fimD"
                     /locus_tag="OO566_002757"
     CDS             complement(13927..16563)
                     /gene="fimD"
                     /locus_tag="OO566_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6605979.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSIMAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQFYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(16630..>16770)
                     /locus_tag="OO566_002758"
     CDS             complement(16630..>16770)
                     /locus_tag="OO566_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone FimC"
                     /protein_id="HCR6605980.1"
                     /translation="RVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALTPKMTGV
                     ME"
BASE COUNT         4074 a         4313 c         4082 g         4301 t
ORIGIN      
        1 gaccgcctgc cgtaaatcac tttaatttca gcagttgtta ttaaaggata cccttttacg
       61 agggtatccc ttcagactga taacaaacgc tacattgccg gatgtgctgc gcttaccagg
      121 cctatataac ccctgcaata tattgaattt attgtttttg taccccagtc ggcataaacg
      181 accactcaag aaactcaaca gcaaccgttg atgtcagcca gaccccgttt tccgcttgcc
      241 agaaagggat gcctcgtttc gccatttcct gtgctttgac ggttaagaca accggcgatc
      301 cgtgtcttgc tcccactttg cgtgccgttg cttcatcagc agagagatgc acgtaatgac
      361 gatcccctgc aatcagcccc tgttttttta tctcgtcaag gaaacggctt gccgtaccgt
      421 ggtagagaaa ctgtggcggt gttttttcgg caaaggaaat agcgacctgc gaagtcgaat
      481 gcccctgaac cgcgcggatg cacctaccat cactggaata actaaaacgc tttttatcgc
      541 tggttgcgac cacggtatcc agcaatgcac gggttaatcg cttgcccgct ttttgggcac
      601 aaagtatgag cttattgata tccgcccatc cttcacggtc cagtacgata ccaatcgcct
      661 ccggtttgtg tcggaggaca aaacttaaaa acttgctcgt atccgccagc tctttttcgt
      721 tgtactttgc catcgcgttt tcgtccctgg attttctgtt tttatttact aatgtcattg
      781 tcactgggtt tatgaatttt ctgcaactca atcctgcgca gaaccttttc cacacagcgt
      841 aatttttccc ctatgcaaaa aacgcatagc cagaaacata cttgcatttt ccccataccc
      901 acatggctga taaggttttt cagttccagg aattttcctg gctcaagcaa cacacaaaca
      961 caaaaaacaa tggggattgt tatgacacaa caaggggatg ctgtagccgg ggagctggcg
     1021 acagaaaaag tgggcattaa agggtatctg gctttttttc tcaccataat ctttttttcc
     1081 ggtgtttttt ccggaacaga cagctggtgg cgcgtttttg atttttccgt tctgaacgga
     1141 tcgtttgggc aactgcctgg tgcgaatgga gccaccacct catttcgcgg ggtgggtggc
     1201 gcgggggcta aagatggctt tctctttgcg ctggagctgg caccgtcagt cattctctct
     1261 ctggggatta tttcaattac cgatggtctt ggtggattac gcgccgcgca gcagttaatg
     1321 acacctgtgc taaaaccgtt gttgggcatt ccagggatct gctcgctggc actgatcgct
     1381 aacctgcaaa acacggatgc cgccgccggt atgaccaaag agctggcgca ggaaggtgaa
     1441 attaccgaac gcgataaagt gattttcgcc gcatatcaaa ccagcggtag cgccatcatc
     1501 accaactact tctcttccgg cgtcgcggtg tttgcctttc tgggcacgtc cgtaatcgta
     1561 cctttagccg tcatcctggt gtttaaattt gtcggagcca acattttgcg cgtctggctt
     1621 aacttcgaag aacgtcgcaa cccaacgcaa ggagcgcaag catgacaact caggtccgca
     1681 aaaacgtcat ggatatgttt atcgatggcg cgcgccgtgg ctttactatc gccacgacca
     1741 acctgctgcc aaacgtggtg atggcgtttg tcatcattca ggcgctgaaa attaccggcc
     1801 tgctcgactg ggtggggcat atctgtgaac ccgtaatggc gctgtgggga ttacccggtg
     1861 aagcggcaac cgtcctttta gccgcattaa tgagtatggg cggcgcagtc ggtgttgccg
     1921 ccagtctggc aaccgctgac gcattaaccg gccacgatgt caccgtttta ctgcccgcaa
     1981 tgtatttaat gggcaacccg gtgcaaaatg tcggtcgctg cctggggaca gccgaagtga
     2041 atgccaaata ttacccgcat atcattacgg tctgcgtgat taacgcattg ctgtcgatct
     2101 gggttatgca gcttattgtt taacaaggag ttaccatgat tgattatacc gcagccggtt
     2161 ttaccctgct gcagggagcg catttgtatg cgccggaaga tcggggaatt tgcgatgtcc
     2221 tcgtcgctaa cggcaaaatt atcgccgttg ccagcaatat cccttctgac attgtaccga
     2281 actgcacggt tgtcgatctc agcgggcaga tcctctgccc aggttttatt gatcaacacg
     2341 tccatttgat tggcggtggc ggcgaagcag gtcccacgac gcgcacgccg gaagtggcgc
     2401 taagtcgcct gacggaagcg ggcgtcacgt cagtggttgg tctgctgggc accgactcta
     2461 tctctcgcca cctggaatcc ctgctcgcca agacccgtgc gctcaatgaa gaaggcatca
     2521 gcgcctggat gctgaccggc gcttatcatg tcccttcccg cactattacg ggttccgtgg
     2581 aaaaagacgt ggcgattatc gatcgtgtga ttggcgtgaa atgcgccatc tctgatcacc
     2641 gttctgccgc accggacgtt tatcacctgg ctaatatggc ggcagaatcc cgcgttggcg
     2701 gtttgctcgg cggtaaacct ggcgtcaccc tgttccacat gggcgacagt aaaaaggcgt
     2761 taaagcctat ttatgacctg ctggaaaact gcgatgtgcc gatcagcaag ctgctgccga
     2821 cccacgttaa ccgcaacgta ccgttgtttg agcaggcgct ggagttcgcg cgcaaaggcg
     2881 gcaccatcga tatcaccagc agcattgacg aaccggtcgc ccctgccgaa ggtattgccc
     2941 gcgccgttca ggcgggtatt ctgctggcac gcgtcaccct cagctccgac ggcaacggta
     3001 gccagccgtt cttcgatgac gaagggaatt taacccatat cggtgttgca ggttttgaaa
     3061 cgttgctgga aaccgtgcag gtgctggtca aagactatga tttcagtatc agcgatgccc
     3121 tgcgcccgct caccagtagc gtagccggtt tccttaacat gagcgggaaa ggcgaaattc
     3181 tgccaggcaa tgacgcagac ttactggtca tgacgccaga attgcgcatt gagcaggtat
     3241 acgctcgcgg caaactgatg gtcaaagacg gcaaagcctg cgtgaaagga acgtttgaaa
     3301 cggcttaaac ggttgtcagc cgccctaatg gacgtagaat gcccccaagg gcggctgaca
     3361 gagtaaaacg taatggatga ctgtggtgcg gttttgcata atattgaaac caaatggctt
     3421 tatgattttc tgactctgga aaaatgccgc aatttttccc aggcggcagt cagtcgcaac
     3481 gtctcgcaac cggcattcag ccgccgcatc cgtgcactgg aacaggcgat tggcgttgaa
     3541 ttgtttaacc gccaggtgac gccactgcaa ctctcagaac aagggaaaat cttccattcg
     3601 cagatccgcc atctgttgca acagttagaa agcaaccttg cagagctgcg tggcggcagc
     3661 gattacgcgc aacgtaaaat caagatagcc gctgcacact ctctttccct cggtctgtta
     3721 ccgtccatta tcagccagat gccgccgctc tttacttggg caattgaagc cattgatgtc
     3781 gatgaagcgg tcgataaact gcgtgaaggg caaagtgact gtattttttc ctttcacgac
     3841 gaagatttgc tggaagcgcc gtttgaccac attcgcttat ttgaatctca attgttccct
     3901 gtctgcgcca gtgacgaaca cggagaagca ctttttaacc tcgcgcagcc acactttccg
     3961 ttactgaatt acagccgcaa ctcctacatg gggcgattga ttaatcgcac cctgacgcgc
     4021 cacagtgagt taagtttcag cgcctttttt gtctcttcga tgagcgagct tttaaagcag
     4081 gttgccctcg acggctgtgg gattgcctgg ctgccggagt atgccatcca acaagaaatt
     4141 cgcagcgggc agctcgttgt gctgaatcgg gacgaacagg tgatcccgat tcaggcctac
     4201 gcataccgga tgaatacccg catgaatcca gttgccgaac gcttctggcg tgaactgcgc
     4261 gagctggaga ttgtgcttag ctgacattct caagcgtcgc actgcgccaa ccgccattat
     4321 cccagaccag atcaaacgtt agcggttcat cgtcgtggta cagcattatc tcaaggctga
     4381 tcgccagagt gtggcagtac gcttctttca aattaacctg caacgccgca atccgtggct
     4441 catgaaataa cagtgcagaa cgcagctgac ggcaatacca gctctggaga tgtttgtcgc
     4501 tcgggttatg cacttgccac tcgggcatgc ctgaaacaag taatgacggt aatgccgaat
     4561 gaggagaatt aaaaagagta aggagatcgc tgcataccga tttacgcagc aatgacgttt
     4621 tattgcctga aatttcaggt aaaacagcct gaagtgattg gcgcatcatt ttgcgcactc
     4681 ctgacgttta gcaacatatg cggcttgccc tgaacaccgg gcaccactgt attgatgctc
     4741 attttccata gccattccct tttgttcatt acatccatgt cagtctgaga caggcctgaa
     4801 tcctgtcagc aaagtataaa accttacttg cctatgaata tctactgact tttcaggaaa
     4861 aaagtcatga tttgataaaa atcatcaaac tcaaaaaaaa accgctagta tattctgaat
     4921 attccgaata gttacgtaga tgaaaatact tgtcaccgct gctacttaat tccggtggga
     4981 aattatttat ttccgcataa tcagccatgt attatttgaa acaattagtt tgtaaataat
     5041 ataaattgat aacatatcaa atacatcaaa aataccattc acaggcatta ttaatataga
     5101 cctcaatata gcaacatcaa attccaatta gtaataagat atgcttttaa attgcatgca
     5161 aataatttta aatgagcgtt ttttgatact ttatagtgtt tcctaccctt taatatcact
     5221 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
     5281 ctacagaaaa atttatctat ttttctaaac atcagttaaa tactcataaa aggtaactat
     5341 tatgtctatg ccattaagca atgccgctaa taaccaattc aatacaaata atcacttcct
     5401 tcatcatcca aaagtcgaca gtgagttaac acgtcgatat gaatatgcac gcctggatac
     5461 ggaaaatatc tatcttctgc ctcttgctcg tggcaataac cataattacg atggaaaaag
     5521 cgttgtcgaa attagaaaac ttaatattgg aaaacagtcc tggccattta attatgtgac
     5581 agatacatgc agagaattca atggtattac aacaacagga cgtatgctct atcgaaactt
     5641 gaaaatcacc tcagctttag acgaaatata tggtggtata tgtaaaaagg cgcatgctgc
     5701 tacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
     5761 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
     5821 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
     5881 tgagcagcca agaattgata tctatattga tcagataata actgtctcag gggtagcaaa
     5941 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagatgg gaaatgaccc
     6001 aatcattggt atagaagcct atcctggtac cgcggagatc cattcaaaaa tgggatataa
     6061 cgttatcccc ggtgatgaag atgcgccact caaacgcatg actctgcaac cgtcatctct
     6121 tccagaactg tttgaattaa aaaatggtga atggaattat attggtaaat agccactgag
     6181 aagaatgagt actaatattc aggttataca gatatttaaa caaaaccctg caatttacaa
     6241 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacgttta cccttgcgct
     6301 tattataata agtcaggcgt cgactttatc cagcggccat gaatcaaaga gatagccgtc
     6361 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     6421 ctgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     6481 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     6541 gatccacatt ggattgtttt cccgccactg ccaggactga cggaataact ccaccagcat
     6601 gctgttatgc gttgcttcag caatggcgag atggaactgc atgtcaccgc tttcgctgct
     6661 acccggcgcg ctggaagcca gttcacgctc ttccagttgc aatgcctgac gcattttgac
     6721 gatatcttcg cgggtagcct gcagtgcggc aaactcggcg atgttacttt ccagtaactg
     6781 acgcgcctgt aacagctcaa aaggacccgc atcattgcag acgttggcat ccggactgtc
     6841 tgtgttgtgg ctgcctgagc tatcaagcac atagataccg gcaccccggc gcacttccac
     6901 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtccgcg tgacatcaag
     6961 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtacg gcgtctttat
     7021 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcacag aggtgacaga
     7081 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     7141 cgccttatcc ggcctacaac gggcaatttt acggcccccg ttaatctcaa actataccat
     7201 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     7261 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     7321 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     7381 cattctgcgg cagatcatcg gcaaaaatac cgctcaggcc cagcagcgct ttcacgcggt
     7441 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcgaccacgt
     7501 caatggcatt accctgctca tccacgccct gggtgtaacg catccagcca gccacgccca
     7561 gcgtcaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccggg tccagtagac
     7621 gctgcggtaa cttctggctg ccgtccatcg caatctgcca ggtacggtga cgcagagacg
     7681 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtccgta ccttccggca
     7741 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     7801 ggttagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     7861 aagagtggct gccgttcagc atacgcagct tcatcatttc gaacggcaca acgtctgcaa
     7921 cgaactgtgc gcccacttta tcccagtccg ggcgaccatt aacgaagtta tcttcaatca
     7981 cccactgacg gaacggttcg caggcaatgg cgcatgggtc gtaaacaccc agctggtcag
     8041 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     8101 ggaaggtgac gttctcttca atccatgccg ctagttgcgg gtcacgcgcc tgagccagcc
     8161 ccagtaccgc gaccttcgcc acatgaccgt tttcacgcac gttatcgcag gacatcaccg
     8221 taaatgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     8281 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     8341 gttgaccgct tgccgcatca gcgcagtagc ctttttccgt gaccgttaga gagacaatcg
     8401 ccgtttgcgg acgcgccatc gcgttgagaa taccttcgca gccgtcgatt tccggatgca
     8461 gcgcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcta
     8521 cggtgtacag cagttgctgt tttttcaggt tttcgatcag catacggtcg ttgcctggca
     8581 tcaggttaac ttcgcagatg ccccagtcgc tgtcggtgct ttccagcaga tgatgggtat
     8641 acaacgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     8701 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     8761 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     8821 gcgcagccat gccggtgggt attaacggct aaagaaagcg cgctggatcg ccagttcgac
     8881 accgcgaact tcggccaggc ctttcagacg accaattgcg gagtaaccag ggttggtttt
     8941 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     9001 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     9061 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     9121 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     9181 atcgttgtca gcacgcacgc cgtaggaacc ggtgcacatg gtgaaaccgt tcgccatgct
     9241 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaagcc
     9301 gaggatcggg cgcggcggat cgtccgggtg aaccgccata cgcacgccaa cttcttcagc
     9361 aactggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     9421 atctttgtac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     9481 cggcagaccg gcaatgatgt tacgcgtcag acgcgctttg tcttcgtcgc tcatagtggc
     9541 gaagcgttca gctgcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     9601 tttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     9661 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     9721 gtagcacacg gtgcgaatgc cacactgcgc caggttgcgc agagtctgct gatagttagc
     9781 gatccactgc tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gtacgctttc
     9841 tacgacagac cacaccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     9901 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     9961 cgcctgacgg acatcagcta aagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
    10021 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgc ggaaccttgg
    10081 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
    10141 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaactctgaa attggttaac
    10201 cacatcacaa gaatttcact ctcatggttg ttgctgcaaa ttgcagcaaa gggtaatcga
    10261 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttgcatgaca
    10321 tgtcgcccaa aataacaacg gttcaaccaa gtaaggtaaa acgttatgca tgtgcttaac
    10381 attctctggg tggtattcgg cattggcctg atgctgatac tgaatttgaa gttcaaaatc
    10441 aattcaatgg tggctttgtt ggtggcggcg ctgtccgtcg ggatgctggc aggcatggat
    10501 ttgatgtcgc tgctgcacac catgaaagcg ggcttcggca acacgctggg agaactggct
    10561 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggctcac
    10621 cagatagcac atacgctgct ggcgcgtcta ggtctgcgct atgttcagct gtcggtgatt
    10681 atcatcggcc ttattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
    10741 ccgctggtta ttgttattgc cgccgaagct aaaattccgt tcctgaaact ggcgatcccg
    10801 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
    10861 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
    10921 atcccaagtg taatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
    10981 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
    11041 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
    11101 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat tggttcctcg
    11161 ccgattgcga tgtttattgc gatggtggtt gcattcgtac tctttggcac cgcgcgtggt
    11221 catgacatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagtat tgcaatggtg
    11281 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggc
    11341 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacattat ggcatggctg
    11401 atcactgtgc taattcgtct ggcgacgggt cagggtgtcg tttcggcgat gaccgccgcc
    11461 gggattatca gtgctgcaat ccttgatcca gcaaccggtc agctggttgg cgtgaatccg
    11521 gcgctgctgg tactggcgac ggctgcgggt tccaacaccc tcacccacat taatgatgca
    11581 tctttctggt tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
    11641 ggtctgctgg agctggtcaa ctccgtggtt gggctgatta ttgtgctgat tattagcatg
    11701 gtagcgtaaa agaacaccag agcctgccaa tggcaggctc agactgatga ctaaagtcta
    11761 aaaatgtgcc cggacagtcc cctcgcccct ccggggagag ggttagggtg aggggaacag
    11821 accagtaaag tgcgctttgt ttatgccaga tgcgacgctg acgcgtctta tctggcctac
    11881 aaagggctaa cgtgcaggtt ttgtaggtct gatagcgtag cgcctcaggt accagcatta
    11941 gcaatgcgct gtaatttttt tattgataaa caaaagtcac gccaataatc gattgcacat
    12001 tccctgcagt cacctgccct ccggtacgtg cgtaatttgc cgttaatccc agacttaccg
    12061 ccgaagttcc tactgctcct aacgataccg tgttattcgt tggaataatc gtaccgttgc
    12121 gcgtcaactg tacgccgacg ccctgcgctg gtgaaaacga cgcggtattg gtgaaaatcg
    12181 agttgcccgc atctgcggtt gtgccggaga ggtaataccc caggttttgg cttttcgcac
    12241 aataaacggt aagaggaatt ggcactgaac cagggtagtc cggcagagta acggtgacat
    12301 cacgagcaga aacatcacaa ccgccagtgg gcaccaccac atcattattg gcgtaaatat
    12361 tccacacaaa ctgaaaatca tcgctgttat agttgttggt ctgtcgcaaa ataagcacgg
    12421 caattaatga accagcttta atcgccaccc cgcccgcact gctcacaggc gtcaaataaa
    12481 gcgccaccgg ccacggctta tccgttctcg aattataaac aacccgcggc gtttcgctgg
    12541 tggtcggaaa tggatagcta ctgccactat attttacggt cccggaaaaa ttagataaca
    12601 cgccgccgta agccgagcct cgttgcagtg tgacatagtc tgtaatggtt tccggataat
    12661 cgttatggca aaagatttgc gtcgaaagat ctacgaccag gttttgcccc acattcacga
    12721 caggcgcaag gtttacataa acattagcgc tgccaccgcc aatagggata gcggtaccat
    12781 tggcggtttt acaggcgaat gaccaggcat ttaccgacca gcccatcagc agtacagcaa
    12841 acagggtaat agctcgtttc attacaatta tctcttcggg ttcagctgta ggtataggtg
    12901 atgctaatca ctgcctgaat ggttccctga gtggctccgc catttaccgt caatgctctg
    12961 acctgtaacg ggaagtgcgc tgattgtgag gaatcatcca cctgaactgt tttggttgcg
    13021 ccagtattca atgtgttgcc actgtcatcc tgtagctcta actggatgtt ttgcgcggtc
    13081 ccctggtttt tataatatcc ggtgctgtcg gctgccccgc tgaagctggc agtgaccctc
    13141 gacgttccca ccggacaatt agtcaactca agcgcaacat catgccaggc cgatgccgcc
    13201 ccggcagaca taagactgaa agaataaaga tcgccgagat ctaccgtggc attggtggtg
    13261 gaaaccgtac acggtttggc gacgacctta ccgttcaccg tgatggtgac atcggctgcc
    13321 tgtatcgtcg cacttgcgag cgccaacatt gccgccaata catacccacg tttgcaccat
    13381 ttcatgagca tctccagtta ctgatattca agagtgaagg tagccgtagc attgatatgc
    13441 cccgcagtga caggcacctg tgtcgccatt agccgggcgt aaaaattcag cgtatttggt
    13501 ttacccggcg tcagggtcgt ccacgaaatc gcagacgatg gagcattaag gggtatttga
    13561 ttttgctgct cattcagaag ctgtattccc agtcccgcag ccgctgacac cgtattttca
    13621 agtgcaagca ggttggcatt gtggctatct gcaacgccgg taaacccaac ttttacggca
    13681 gaaacagggt gacagcaaaa tacgaaatgg aacgacagga gtcgtcgcgc caatgttgtt
    13741 aaattgcttc gccgcgtttt ccatcagatc aacagtaaaa ttggttgatt cagcggccac
    13801 actacagccg ttatctctga catagccgcg gatagtaatc gtgctatccg cagccaaaac
    13861 gcgacttacc gccagccaca aaaaagcgca cagaagataa aaaggtttgt ttctcatcac
    13921 gcccccttaa cgacattcag ctgatagctg ggttaataac tgctgctgac tctctggtgg
    13981 cagttgataa ttggcgacac agtgagcatt ttcctcttct ccccatttca cctgaacttt
    14041 tcccgctaaa ggcattccgc tgaggtaaaa ctgaccatta tccgcaacaa tgccgctact
    14101 ctggctactc tctgatgtca ccatcgcccc aaacggcagc ggcttattat tgtgagttag
    14161 cgtcatgagc agttttatcc caacgcgcgc tttaaactct gctcgcacga tcgccccacg
    14221 agtgggaaca acgttagcga ccgcgttatc taaatcgacg ttatcagcca aggtattggt
    14281 atccagcgcc actctatttt cccgatattc agtggcataa ggcagcacgg cataaccacg
    14341 ccagtcggta cgcacccccg tctggttttc gacttttgca tcttttgcgc caggcgcttt
    14401 aacaagcacc accgtatcgt ttaacggctg ccccagcgtt acgccattgg catgagccaa
    14461 taccccaccg ctgactccgt aatagagctg cttaatatca tcgctatggc tgtaaccgat
    14521 attggcattg ccgtaaccac cgcgataatt cagcgtggcg tagcctgtac ttccgctatt
    14581 accatcgcct cccccggcat agccggtttg cacgctataa ctgaggttgt tgtcttccag
    14641 caacgtaccg tatacaccag ccagattggt catccgaccg ttgagatcgt gtgacatgct
    14701 gtagctggca ctggcatgtc gccactgaga tttactgtca gaacgcagcc agtggctgaa
    14761 aggaatattg acgttaagcg ctaacatctg atcccgtcct ttttgccagg cgtttttcgt
    14821 caggctatag ctgagcgtcc agttgatatc ttcgaacgca gtatttaatc cagcctggaa
    14881 ttgctcatcg acattactcg ttccccaata agtttgatgg ctaccactca aatacagtgt
    14941 tgatgtgcgc ccgagttgct gagtaacggt gagctgtaat ttcccgcgtt tgttataagc
    15001 gaggttgtaa tagtcggtga atttcggctt aacctgaata actccatcct gtgtttcgat
    15061 gttgtagcca ttcattcgac tgtatgttgt atcagcgaaa ttaaaatatc cgctggtcga
    15121 ataacggtaa cccactaact gaatattcgt gcctgattca ttgagcgatt tgttatagag
    15181 aaaacgcacc gattgtccgt catgctgact gtcatcggga agtgtggaat tagcctgcgt
    15241 catatccaca gacagagcgc ccagtgcctc catgtttttc ccgataccga aattaaaagc
    15301 acgataacga tccgccagtt gcgttccacc atatattgtc cagccagccg gaaggccgtg
    15361 gagtaatgta ctctggaaaa agcggggttt ttcctgctgc gcatttccac tacggtattc
    15421 tcctgccata atggaataac gagtatgccc ttcacgttgc aaaagcggga ctgacgaata
    15481 gggtacggta aaaatctgcg tgctgccgtc agcctctttg atcgttacct gcaagtcacc
    15541 actattacct gcggcataga tatcgttgat ggtaaaaggc cccggtggca ccgtactatt
    15601 ataaatgcca tatccatttt gtttaatagt gacctgtgca gtaccatgag caataccgtg
    15661 gatcaccggg gcaaatcctc tttggctatc gggtaacata ttgtcatctg aggccaattg
    15721 tgcgccgcga aagttaatac catcgaaaat atcgccctgg gtataaccat cacccaacgt
    15781 cagccgggaa cgtaaaggaa ttatgtctcg ctcaatccag gtaatgatat gctgccattt
    15841 atttttgcta cctgatgaac tgtcgctact gttataactc caggtggtat tgtcgcgtaa
    15901 acgccacgca ccaatattta acccactctg taggtttaaa tatgcataat ggctgttacc
    15961 cccaatccga ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat
    16021 accgggatcc cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg
    16081 agggatcgtc aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac
    16141 cattgtggtt aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc
    16201 cgtattcagc cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc
    16261 actgtcgccc gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat
    16321 atcgacgcga tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc
    16381 cacagcctgg ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa
    16441 aggtgcctgt gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg
    16501 atgcttacga atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga
    16561 cattattcct ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt
    16621 ccccctgcgt tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat
    16681 tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat
    16741 tggaggcacc aatgcatttt caagaacccg
//
LOCUS       DAJTXY010000081        16727 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTXY010000081 DAJTXY010000000
VERSION     DAJTXY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.081"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO566_002759"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO566_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6605981.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO566_002760"
     CDS             2219..2767
                     /locus_tag="OO566_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6605982.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO566_002761"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO566_002761"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6605983.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO566_002762"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO566_002762"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6605984.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO566_002763"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO566_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6605985.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO566_002764"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="OO566_002764"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6605986.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="OO566_002765"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="OO566_002765"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6605987.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12382
                     /gene="pepN"
                     /locus_tag="OO566_002766"
     CDS             9767..12382
                     /gene="pepN"
                     /locus_tag="OO566_002766"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6605988.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12589..13356)
                     /gene="ssuB"
                     /locus_tag="OO566_002767"
     CDS             complement(12589..13356)
                     /gene="ssuB"
                     /locus_tag="OO566_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6605989.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13353..14000)
                     /locus_tag="OO566_002768"
     CDS             complement(13353..14000)
                     /locus_tag="OO566_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6605990.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNI
                     DRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGI
                     GYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(14012..15157)
                     /gene="ssuD"
                     /locus_tag="OO566_002769"
     CDS             complement(14012..15157)
                     /gene="ssuD"
                     /locus_tag="OO566_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR6605991.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15154..16113)
                     /gene="ssuA"
                     /locus_tag="OO566_002770"
     CDS             complement(15154..16113)
                     /gene="ssuA"
                     /locus_tag="OO566_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6605992.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTIGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16106..16681)
                     /gene="ssuE"
                     /locus_tag="OO566_002771"
     CDS             complement(16106..16681)
                     /gene="ssuE"
                     /locus_tag="OO566_002771"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6605993.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4176 a         4316 c         4283 g         3952 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agtcgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg agaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaattatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgtgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atgggggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ctcttccagc caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccagcgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagaa gagggcgcac tggtcatcag
    10021 taatttgccg gagcgtttta cgcttaagat cattaatgaa ataagcccgg cggcgaatac
    10081 ggcgctggaa gggctttatc agtcaggcga tgcgctttgc acccagtgtg aagccgaagg
    10141 tttccgccat attacgtatt atctcgaccg cccggacgtg ctggcgcgtt ttaccaccaa
    10201 aattattgcc gataaaatca aatatccctt cctgctttcc aatggtaacc gcgttgcgca
    10261 aggcgaactc gaaaacggac gccattgggt acagtggcag gacccgttcc cgaaaccgtg
    10321 ctacctgttt gcgctggtgg caggcgactt tgatgtactg cgcgatacct ttaccacgcg
    10381 ttctggtcgc gaagtagcac tggagctgta cgtcgatcgc ggcaaccttg atcgcgcgcc
    10441 gtgggcgatg acctcgctga aaaactccat gaaatgggat gaagaacgct ttggcctgga
    10501 gtatgacctc gacatctata tgatcgtcgc ggtggatttc ttcaatatgg gcgcaatgga
    10561 gaataagggt ctgaatatct ttaactccaa atatgtgctg gcccgcaccg acaccgccac
    10621 cgacaaagat tacctcgata ttgaacgcgt tatcggccat gaatatttcc ataactggac
    10681 cggtaaccga gtgacctgcc gcgactggtt ccagctcagc ctgaaagaag gtttaaccgt
    10741 cttccgcgat caggaattca gctctgacct tggttcccgc gcagttaacc gcatcaataa
    10801 tgtgcgcacc atgcgcggat tgcagtttgc ggaagacgcc agcccgatgg cgcacccaat
    10861 ccgcccggac atggtcattg agatgaacaa cttctacacc ctgaccgttt acgagaaggg
    10921 cgcggaagtg atccgtatga tccacaccct gctgggcgaa gaaaacttcc agaaagggat
    10981 gcaactctat ttcgagcgtc atgatggtag cgcagcgacc tgtgacgact ttgtgcaggc
    11041 gatggaagat gcgtcgaatg ttgatctctc ccatttccgc cgttggtaca gccagtccgg
    11101 tacgccggtt gtgaccgtca aagacgacta caatccggaa actgagcagt acaccctgac
    11161 catcagccag cgcacgccag ccacgccgga tcaggcagaa aaacagccgc tgcatattcc
    11221 gtttgccatc gaattgtatg acaacgaagg caaagtgatc ccgttgcaga aaggcggtca
    11281 tccggtgaat tccgtgctga acgtcaccca ggcggaacag acctttgttt ttgataatgt
    11341 ctacttccag ccggtgcctg cgctgctgtg cgaattctct gcgccagtga aactggaata
    11401 taagtggagc gatcagcaac tgaccttcct gatgcgtcat gcgcgtaatg atttctcccg
    11461 ttgggatgcg gcgcaaagtt tgctggcaac ctacatcaag ctgaacgtcg cccgtcatca
    11521 gcaagggcag ccgctgtctc tgccggtaca tgtggctgac gctttccgcg cggtgctgct
    11581 cgatgagaag attgatccgg cgctggcggc agaaatcctg acgctgcctt ctgtcaatga
    11641 aatggctgaa ctgttcgata tcatagaccc gattgctatt gccgaagtac gcgaagcact
    11701 cactcgtact ctggcgactg aactggcgga tgagctgctg gctatttaca acgcgaatta
    11761 ccagagcgag taccgtgttg agcatgaaga tattgcaaaa cgcactctgc gtaatgcctg
    11821 cctgcgcttc cttgcttttg gtgaaacgca tctggctgac gtgctggtga gcaagcaata
    11881 ccatgaagca aacaatatga ccgatgcgct ggcagcgctt tctgcggcgg ttgctgcaca
    11941 gctgccttgc cgtgacgcgc tgatgcagga gtacgacgat aagtggcatc aggacggtct
    12001 ggtgatggat aaatggttta tcctgcaagc caccagcccg gcggcgaatg tgctggagac
    12061 ggtgcgtggt ctgttgcagc atcgctcatt taccatgagc aacccgaacc gtattcgttc
    12121 gttgattggt gcgtttgcgg gcagcaaccc ggcagcgttc catgccgaag atggcagcgg
    12181 ttaccagttc ctggtggaaa tgcttaccga cctcaacagc cgtaacccgc aggtagcttc
    12241 acgtctgatt gaaccgctga ttcgcctgaa acgttatgat gccaaacgtc aggagaaaat
    12301 gcgcgcggcg ctggaacagt tgaaagggct ggaaaatctc tctggcgatc tttacgagaa
    12361 gataactaaa gcactggctt gatacttttg agccaaaatg cctgatgcga cgcttgacgc
    12421 gtcttatcag gcctacaact ccgttgaaat tgctggtttt gtaggctgga taaggcgttc
    12481 acgccgcatc cggcagccgt gcccaactgc ctgatgcgat gcttgacgtg tcttatcagg
    12541 cctacaagtc cgttgaaaat gctggttttg tgcgccacat ccggcatatt aaccatgacg
    12601 ccgaatcagt tgttcagaat gcccacgccg cattacccgt tgcaatactt ccgcttccag
    12661 ctccgctaat cgcaccgacc ctaatcgacg cggacggggg atatccactg tcaaatccag
    12721 actaattttt ccctcttcaa ttaacaacac ccggtcagcc attgctaccg cttcgctcac
    12781 atcatgcgtc accaacagta cggtaaagcc gtgctgctgc caaagtgaca caatcaaatc
    12841 ctgcatctcg agtcgcgtta aggcgtccag tgccccgagc ggttcatcaa gcaacaataa
    12901 acccggtcga tgaatcaacg ctcttgccag cgccacacgc tgtttctgcc cgccagaaag
    12961 tgcggcaggc cattccccgg cgcgattctc cagtcctacc gcagccaacg cccgacgcgc
    13021 ggcatcgcgc cactgacctt taaggcctaa cccaacgtta tcaatcaccg atttccacgg
    13081 cagcagacgc gcatcctgaa acatcattcg cgtatcatcc tgaatttcag ccagcggtgt
    13141 ggtgcccgct aacacatcgc ctgcggttgg cgtttccaga ccggccagca ggcgcagcag
    13201 ggtactttta ccaccaccgc tgcggcccac caccgccaca aactgacctg ccggaatatg
    13261 taaatccagt tggttcagga cgatattttc cgcgtaatgt ttgcttactg cattgagcaa
    13321 caatggtgtg ccctggttca gacgagcagt attcatatcg tggcctcctt caaatgataa
    13381 gccgggttcc agctcaacca gaggcgctct aacagctgcg cgctgacgtc agcaagtttg
    13441 ccgagcaggg cgtaaagaat aatagcgact accaccacgt ctgtttgcaa aaactctcgc
    13501 gcattcatcg ccagataacc aatgccggaa ttggcagaaa tggtttcggc aacaatcagc
    13561 gtcagccaca tcaggcccaa cgcaaaacgc acgccgacca taattgaggg cagggcatcg
    13621 ggcaggatca catggataaa cagcggtatg ccggataatc catagctacg cgccatctcc
    13681 accagcccgc gatcgatatt acggatgcca tgccaggtgt tgatataaat ggggaacagc
    13741 gtaccgagcg ccaccagaaa gattttcgcg gattcatcaa tgccaaacca caaaatcacc
    13801 agcgggatca gcgccagatg ctgccacagt tcgccgctgg cggagagcgt ccagaacgcc
    13861 gtcaccactc cttccggtga aggcaaaata cgcgtcgaca gccagccaac cgaggaggcc
    13921 agttgccaca ccgccacgat gcccaccggt aaaaaccagg gggcaacgcg caataaccac
    13981 ttcttcactg gcgttgccat catcgtgttc cttagctttg cgcgacttta cgggggataa
    14041 aatcattcgc caccgcttcg ccttgcggat tcagcggctg cggctgggga atttccggaa
    14101 tggcgacatc cagatgtggg aacagcaact cgccaacccg atacgcttct tccagatgcg
    14161 ggtagcccga aagcacaaag ctgtcgatgc caagcgcggc gtattcgttg attcgcgcgg
    14221 cgaccgtagg accatcgccc accagcgccg ttccggcacc gccgcgcact aagccaacgc
    14281 ccgcccataa attggggctg atctccagat tgtcgcgctt gccgttatgt agcgccgcca
    14341 ttcgctgttg tcctacggaa tccgtccggg cgaatgcggc ttgtgctttg gcgatagtct
    14401 catcatcaag atgcgagatt aaccgctcgg cggcctgcca cgcttcgtcg ttagtttcac
    14461 gaacaatcac atgcagacga ataccgaaac gaattttgcg cccatacgcg gcagctttcg
    14521 cccgcacttg ttcgattttc tctttaacaa gttccggcgg ttcgccccag gtgaggtaga
    14581 gatcaacttg ttctgccgcc agctcctggg cgacatctga cgatccgcca aagtaaagcg
    14641 gaggataagg ctgttgaatc ggcgggaaga gcagttttgc tccgcgcaca tgaatatgtt
    14701 taccgttgaa attgacggtt tcccccagca ataagcgccg ccagacctgg gtaaattccg
    14761 ccgaggcttc gtagtgctcg ctatgatcaa ggaacacgcc gtcgcctgcc agctcttgtg
    14821 gatcgctgcc tgtgaccaga ttaaacaacg cacgaccatt tgagagacgg tcaagcgtgg
    14881 cggcctggcg ggcggcaaca gtaggtgagg ttacgctggg acgcagggcg acaagaaact
    14941 tcagccgctg cgtcaccggg atcatcgacg ctgcaaccag ccacgcatct tcacacgatc
    15001 gccccgttgg gatcagcaca ccggtatagc ccagacgatc cgccgcttgc gcaatttgtt
    15061 gcagataacc gtgatcaacc gggcgtgaac cttcttccgt tcccagatag tgcccgtcac
    15121 cgtgggtcgg taaaaaccag aacatattca gactcataat tgttttcctt ccagttgagt
    15181 gggctgccag atgcgctggc gaatatcgac ttttttcggc accagacggt tttcataaaa
    15241 cagatctgcc gtttgctgct gtaaggcggc aacctcggcg ttaaccggtt tgatggtggt
    15301 aggagggcga tgatctaagt aagaggcaat caccggtgcc ggtaagccaa tcgtttttgc
    15361 cagtaaagcg atgctttgtt cgcgctggct gcgggttaac gcatcggcct cactaaaggt
    15421 tgccagtacg ccctgaataa aagcgccgtt tttttctgca tagggacgag ctgccagata
    15481 aaacgatcca gtttgattga gatcggtgcc gtctttcagc acccgcacgc cgccctgtaa
    15541 taacgcagcg gagtagtagg gatcccagat agcccaggcg tcaacgttac cttgctggaa
    15601 cgcggcgcgg gcaccggcag gcgtcaggta agtgggctgg atatcggtga atttaagtcc
    15661 ggcctgacgc agtgcacgca gtaaaaggtt gtgtgaactg gaacctttct gaaaggcaac
    15721 tttgtgacct ttaagatcgg ctacggtttt gatcgggctg ttttctgcca ccagaatcac
    15781 ttcggctttg ggcttcggtg gctcgacgcc cacatatacc aaatcagccc cggcagcctg
    15841 ggcaaagatt ggcggaatat ccccggtact gccgagatca atactgccaa cgtttaacgc
    15901 ttccaacatt tgcggacccg cggggaactc cacccaggag atttttgatt gcggatagcg
    15961 tttttccagt aactggtggc tttttgccag caccatacca atactgcctt tctgatagcc
    16021 tatacgtaac gcttcaggcg aggattctgc agcaaccgca aaagtagaga cgctaagcaa
    16081 tcccgccagc gccagtttaa tgatgttacg catgggcatt acctcgcaga gacagaaggt
    16141 caggaacctg aacatcgcgg cggtgcaatg cctgccagaa agtttctagc gcggtatcaa
    16201 gatggggttg cagatttggc gtgaactggg gtctgtgatg gtaatcaatt aattgtgagt
    16261 catcggcaaa cacgccgtgc aggatctcct gagctttcag tgcgcttaaa actggtttaa
    16321 gggcataatc gaccgccagc agatgggcca cggtaccgcc cgtcgccagc ggtagcacca
    16381 ctttgccttg caaagcgcgc tctggcagca ggtcgagcag ggttttcagc gcgccggaat
    16441 aggcggcttt atacacaggc gtggcgacaa tcagcccatc agcctgttgc agctgttcgg
    16501 tgaaggtctt gagtgccgga ctatcgaaac gagcataaag cagatcttcc ggggcgaagt
    16561 tttgcagatt ccagtgataa acctctacat ctaggccatt tagtttttcc cgcgcatatt
    16621 ccagcaagga gctggagcga gaaggaaagc gaggactacc cgccagggtg atgacacgca
    16681 tactctctcc ttataaccaa ttgttctttt ttttgttaac attgata
//
LOCUS       DAJTXY010000082        16673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTXY010000082 DAJTXY010000000
VERSION     DAJTXY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.082"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..2081
                     /gene="pbpC"
                     /locus_tag="OO566_002772"
                     /pseudo
     CDS             1..2081
                     /gene="pbpC"
                     /locus_tag="OO566_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2231..2662
                     /gene="ndk"
                     /locus_tag="OO566_002773"
     CDS             2231..2662
                     /gene="ndk"
                     /locus_tag="OO566_002773"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6605994.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2812..3966
                     /gene="trmG/rlmN"
                     /locus_tag="OO566_002774"
     CDS             2812..3966
                     /gene="trmG/rlmN"
                     /locus_tag="OO566_002774"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6605995.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4251..5264
                     /gene="rodZ"
                     /locus_tag="OO566_002775"
     CDS             4251..5264
                     /gene="rodZ"
                     /locus_tag="OO566_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6605996.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5291..6409
                     /gene="ispG"
                     /locus_tag="OO566_002776"
                     /gene_synonym="gcpE"
     CDS             5291..6409
                     /gene="ispG"
                     /locus_tag="OO566_002776"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6605997.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6520..7794
                     /gene="hisS"
                     /locus_tag="OO566_002777"
     CDS             6520..7794
                     /gene="hisS"
                     /locus_tag="OO566_002777"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6605998.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7812..8432
                     /gene="yfgM"
                     /locus_tag="OO566_002778"
     CDS             7812..8432
                     /gene="yfgM"
                     /locus_tag="OO566_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6605999.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8443..9621
                     /gene="bamB"
                     /locus_tag="OO566_002779"
     CDS             8443..9621
                     /gene="bamB"
                     /locus_tag="OO566_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6606000.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9739..11211
                     /gene="der"
                     /locus_tag="OO566_002780"
     CDS             9739..11211
                     /gene="der"
                     /locus_tag="OO566_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6606001.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11281..11496
                     /locus_tag="OO566_002781"
     CDS             11281..11496
                     /locus_tag="OO566_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6606002.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11493..12863)
                     /gene="xseA"
                     /locus_tag="OO566_002782"
     CDS             complement(11493..12863)
                     /gene="xseA"
                     /locus_tag="OO566_002782"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6606003.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13025..14491
                     /gene="guaB"
                     /locus_tag="OO566_002783"
     CDS             13025..14491
                     /gene="guaB"
                     /locus_tag="OO566_002783"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6606004.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14560..16137
                     /gene="guaA"
                     /locus_tag="OO566_002784"
     CDS             14560..16137
                     /gene="guaA"
                     /locus_tag="OO566_002784"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6606005.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16232..>16673)
                     /locus_tag="OO566_002785"
     CDS             complement(16232..>16673)
                     /locus_tag="OO566_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6606006.1"
                     /translation="QQGKNEEQRQHDEWVAERNREIQQEKQRRANAQAAANKRAATAA
                     ANKKARQDKLDAEATADKKRDQSYEDELRSLEIQKQKLALAKEEARVKRENEFIDQEL
                     KHKAAQTDVVQSEADANRNMTEGGRDLMKSVGKAEENKSDSWFN"
BASE COUNT         3844 a         4166 c         4669 g         3994 t
ORIGIN      
        1 gaagcgctga tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc
       61 tcggtggcgc gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc
      121 acgctcacta tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa
      181 attctccagc tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg
      241 atcttgtatc ttaaccgcgc tccgtttggc gttacgttgc aggggatcgg tgcggcaagt
      301 tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg
      361 gtgttaccgc aagtgcccag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc
      421 gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta
      481 aaagagtcaa gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg
      541 ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat
      601 gccggtcttc aacgacgtct ggaagaactg gcgcaaactg gaaagggcgg ttgccaccgc
      661 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      721 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      781 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      841 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
      901 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
      961 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1021 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1081 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1141 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1201 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1261 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1321 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1381 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1441 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1501 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1561 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1621 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1681 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1741 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1801 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1861 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     1921 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     1981 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2041 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2101 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2161 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2221 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2281 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2341 caaaatgctg cacctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2401 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2461 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2521 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2581 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2641 agtgtgcccg cgcacccgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2701 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2761 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2821 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     2881 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     2941 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3001 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3061 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3121 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3181 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3241 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3301 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3361 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3421 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3481 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3541 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3601 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3661 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3721 tgtaagatca acctgatccc gtggaacccg ttcccgggcg cgccgtatgg acgcagttcg
     3781 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3841 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     3901 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     3961 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4021 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4081 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4141 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4201 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4261 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4321 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4381 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4441 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4501 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4561 aacgccgcaa aaaacgcgac ggctggctga tgaccttcac ctggctggtg ttgtttgtgg
     4621 ttatcggcct gagcggtgcc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4681 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4741 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4801 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4861 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     4921 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     4981 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5041 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5101 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5161 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5221 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5281 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5341 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5401 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5461 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5521 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5581 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5641 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5701 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5761 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5821 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     5881 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     5941 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6001 cgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6061 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6121 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6181 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6241 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6301 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6361 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt gaaaaataat aacgtgatgg
     6421 gaagcgcctc gcttcccgtg tatgattgaa cccgcatggc tcccgaaaca ttgagggaag
     6481 cgttgagggt tcatttttat attcagaaag agaataaacg tggcaaaaaa cattcaagcc
     6541 attcgcggca tgaacgatta cctgcctggc gaaacggcca tctggcagcg cattgaaggc
     6601 acactgaaaa acgtgctcgg cagctacggt tacagtgaaa tccgcttgcc gattgtagag
     6661 cagaccccgc tattcaaacg tgcgattggt gaagtcaccg acgtggttga aaaagagatg
     6721 tacacctttg aggatcgcaa tggcgacagc ctgactctgc gccctgaagg gacggcgggc
     6781 tgtgtacgcg ccggcatcga gcatggtctt ctgtacaatc aggaacagcg tctgtggtat
     6841 atcgggccga tgttccgtca cgagcgtccg cagaaagggc gttatcgtca gttccatcag
     6901 ttgggctgcg aagttttcgg tctgcaaggt ccggatatcg acgctgaact gattatgctc
     6961 actgcccgct ggtggcgtgc gctgggtatc ttcgaacacg taactcttga gctgaattct
     7021 atcggttcgc tggaagcacg cgccaattac cgcgatgcgc tggtggcatt ccttgagcag
     7081 cataaagaaa agctggacga agactgcaaa cgccgcatgt acactaaccc gctgcgcgtg
     7141 ctggattcaa aaaatccgga agtgcaggcg cttctcaacg acgctccggc attaggtgat
     7201 tatctggacg aggaatctcg tgagcatttt gccggtctgt gcaaactgct ggagagcgcg
     7261 gggatcgctt acaccgtaaa ccagcgtctg gtgcgtggtc tggattacta taaccgtacc
     7321 gttttcgagt gggtgactaa cagtctcggc tcccagggca ccgtgtgtgc aggcggtcgt
     7381 tatgacggtc ttgtggaaca actgggcggt cgtgcaacac cggctgtcgg ttttgcgatg
     7441 ggcctcgaac gtcttgtatt gttagtacag gccgttaatc cggaatttaa agccgatcct
     7501 gttgtcgata tatacctggt ggcttcaggt gctgatacac aatctgcggc tatggcatta
     7561 gctgagcgtc tgcgtgatga attactgggc gtgaaattga tgaccaacca cggcggcggc
     7621 aactttaaga aacagtttgc ccgtgctgat aaatggggtg cccgcgttgc tgtggtgctg
     7681 ggtgagtctg aagtggctaa cggcacagca gtagtgaagg atttgcgctc tggtgagcaa
     7741 acggcagttg cgcaggatag cgtagccgcg catttgcgca cgttactggg ttaaggaagg
     7801 agaaggacag cgtggaaatt tacgagaacg aaaacgacca ggtagaagcg gttaaacgct
     7861 tttttgctga aaatggcaaa gcactggctg ttggggtgat tttgggcgtt ggcgcactga
     7921 ttggctggcg ctactggaac agccatcagg ttgattctgc acgctccgct tctcttgcct
     7981 atcaaaatgc ggttaccgca gtgagcgaag gcaaaccgga tagcatcccg gcggcggaaa
     8041 aatttgctgc tgaaaataaa aatacttatg gtgcgctggc ttctttggaa cttgcgcagc
     8101 aatttgttga caaaaatgaa ctagagaaag ctgccgccca gttacaacag gggctggcag
     8161 acacgagcga tgaaaatctc aaagccgtga taaatctgcg tcttgctcgc gttcaggtac
     8221 agctcaagca ggctgatgcc gcgctgaaaa cccttgatac catcaaaggt gaagggtggg
     8281 ctgccattgt tgccgacctg cgtggtgaag cattgctgag caaaggtgat aagcaaggtg
     8341 cgcgtagtgc atgggaagca ggcgtgaaaa gcgatgttac tccggcactg agcgaaatga
     8401 tgcagatgaa aattaataat ttgtccatct gagagggacc cgatgcaatt gcgtaaatta
     8461 ctgctgccag gactgctttc cgttaccctt ttaagcggct gttcgctgtt taacagcgaa
     8521 gaagatgtgg taaagatgtc cccattgcca accgttgaaa accagtttac gccgaccacg
     8581 gcgtggagca cttccgttgg tagcggcatt ggcaacttct attccaatct tcatccggca
     8641 ctggcggaca acgttgtcta tgcagcggac cgcgctggtt tggtaaaagc gctgaatgcg
     8701 gatgatggca aagaaatctg gtctgtcagc ctggccgaga aagatggctg gttctctaaa
     8761 gagcctgcat tactttctgg cggtgtgacc gtgtctggtg ggcatgtcta cattggcagc
     8821 gaaaaggcgc aggtttacgc gctgaatacc agcgatggta ctgtggcatg gcaaactaca
     8881 gtcgcgggtg aagcactttc gcgcccggtg gtcagcgacg gtctggtgtt aatccacacc
     8941 agtaacggtc agttacaagc gctgaacgaa gctgacggcg ctgtcaaatg gacagttaac
     9001 ctcgatatgc cttcgctctc tttgcgtggc gagtctgcgc cggcaacggc ttttggtgcg
     9061 gctgtcgtgg gtggcgataa tggtcgcgtc agcgcagtgc tgatggaaca gggccagatg
     9121 atttggcagc agcgtatttc ccaggcgacc ggttctaccg aaattgaccg tctgagcgat
     9181 gttgacacga ctcccgtcgt tgttaacggc gttgttttcg cgctggccta taatggtaac
     9241 ctgacggcgc ttgatctgcg cagtggtcag attatgtgga aacgcgaact gggttcggtg
     9301 aatgatttca tcgtcgatgg caatcgcatc tatctggtcg atcaaaatga ccgggtgatg
     9361 gcgttgacca ttgatggcgg cgttacgctg tgggcacaaa gcgatctgct gcatcgcctg
     9421 ctgacttctc cggtgctgta taatggcaac ctggtggtcg gtgacagtga aggttatctg
     9481 cactggatta acgtcgaaga tggtcgtttc gttgcccagc aaaaagttga tagttccggt
     9541 ttccagactg aaccggttgc cgctgacggc aaactgctga tccaggcaaa agacggaacc
     9601 gtgtactcta ttacacgtta atcgtctctg tcgttcactt tgaaaacggc tcctggacag
     9661 gggccgtttt cctgttttta acaacgacgc gaatatagcg tgcgttgtct gatgatttat
     9721 aaaaatgagg ctttaaacat ggtacctgtg gtcgcgcttg tcgggcgccc taacgtagga
     9781 aaatccacgt tatttaaccg tctaactcgc acccgagatg cgctggttgc ggatttcccg
     9841 ggtctgactc gtgaccgtaa gtacggtcgt gcggaaattg aaggccgtga gtttatctgt
     9901 attgataccg gcgggattga tggcacagaa gacggtgtag aaacccgcat ggcggaacag
     9961 tcgctgctgg cgattgaaga agcggacgtc gtactgttta tggtggatgc gcgcgcgggc
    10021 ctgatgccgg cagatgaagc gattgccaaa catctgcgct cccgtgaaaa accgaccttc
    10081 ctggtggcaa acaaaactga cggtctggat cccgatcagg cagtggttga tttctactcg
    10141 cttggtttag gtgaaatcta cccgatcgcc gcgtctcacg gtcgtggcgt attaagtctg
    10201 ctggagcatg tgctgctgcc gtggatggaa gatctcgcac cgcaagagga agtcgacgaa
    10261 gacgctgaat actgggcgca atttgaagcg gaagagaacg gcgaagaaga agaggaagac
    10321 gacttcgacc cgcaaagtct gccgatcaaa ctggcgattg tgggtcgtcc gaacgtaggt
    10381 aagtctacac tcactaaccg tattcttgga gaagagcgcg ttgttgttta cgacatgcct
    10441 ggcacgacgc gtgacagcat ctacatccca atggaacgcg atggacgtga gtatgtgctc
    10501 attgacaccg ctggcgtacg taaacgcggc aaaatcaccg atgctgtaga gaaattctcc
    10561 gtaatcaaaa cgttgcaggc cattgaagac gccaacgtgg tgatgttagt gattgatgcg
    10621 cgcgaaggta tttccgatca ggatctctcg ctgctgggct ttattctcaa tagtgggcgc
    10681 tcacttgtca ttgtggtgaa taagtgggat ggcctgagcc aggaagtgaa agagcaggtg
    10741 aaagaaacgc tggacttccg tctgggcttt atcgattttg ctcgtgtgca ctttatctct
    10801 gccttgcacg gcagtggtgt tggtaacttg tttgaatcag tacgtgaagc gtatgacagc
    10861 tccacccgtc gtgtggggac ctctatgctg acgcgcatca tgacgatggc tgttgaagat
    10921 caccaaccgc cgctggtacg cggtcgtcgt gtgaagctga aatatgccca cgctggtggt
    10981 tataacccgc cgattgtggt gattcacggt aatcaggtga aagacctgcc tgattcctac
    11041 aagcgctact tgatgaacta cttccgcaaa tcgctggacg taatgggatc gccgattcgt
    11101 attcagttca aagaagggga aaacccgtat gcgaacaagc gtaacaccct gacgccaacc
    11161 cagatgcgta aacgtaagcg tctgatgaag cacatcaaga aaaataaata attatccgta
    11221 agtattttat ctgccattcc ctctatgaat gtagagggaa tggctaccgg aggaattcat
    11281 atggaactgc attgtccaaa atgtcagcat gtgcttgatc aggataacgg ccatgcgcgt
    11341 tgcccatcct gcggggaatt tattgaaatg aaagctcttt gtccagactg ccatcaaccg
    11401 ttgcaggtgt taaaagcctg cggtgcagta gattacttct gtcagcacgg gcatggcttg
    11461 atatcgaaaa aacgcgttga attcgtgctg gcttaatgaa cctttttacg cgattttttt
    11521 accggctgga tgttttttac ttcactttct atccagccgt cttccagacg tgtggttagc
    11581 atttcacccg ctttaacttg cttcactttt ttcagtacat tgccgtcagt agcagtagta
    11641 acgctgtatc cacgcgccag cgttgacagt gggcttacgg cttcgaggtg cgttactgca
    11701 ttaccgaaac gttcacgcgt ggcgctaagc tgtgcgcgca ggatttctgc taaacgatat
    11761 tccagttgct gaatgcgcgt ttgcgtgcga tgaatcttcg gctgtggatt ttgctgattc
    11821 agccgctgtg ttaatcgctg ctgctgttgc ccggcacgct taagctgatt ttccagcgca
    11881 aagctcatcc gtttttgcag gcgttcaagc atggtttgct ggcgtgccag ccggagctgc
    11941 ggatgctgct gctgcaagcg atgatgaatc tgcgtaaagc gacgcgtacg gttggcgaga
    12001 taataatcca tcgccatctc cagccgttga cgggccgatt gcacctggcg cagtaactct
    12061 tgctgattac ggctcactac ttcagcggcg gcagacggcg ttggcgcacg cagatcggca
    12121 acaaaatcgg caatggtcac atccgtctca tgcccgacgg cgctgacgac cggaatgcgg
    12181 ctggcaaaaa tcgcccgcgc tacgcgttcg tcgttaaaac tccataaatc ttccagcgaa
    12241 ccgccgccgc gcccaacgat caacacgtcg cactcattgc gctgattcgc cagttcaatg
    12301 gcgcgaacaa tttgccccgg cgcgtcatcg ccctgaacgg aggtggggta gatgatcacc
    12361 ggtagagaag gatcgcgacg ttttaacaca tgcaaaatat catgtagcgc agcaccggtt
    12421 tttgaggtga tcacaccaac gcaatgcgca ggggagggaa gtggtttttt gtattgctga
    12481 tcgaacaaac cttcagcctg caacttcgct ttgagctgtt cgtacttctg ttgcagcagc
    12541 ccttcaccgg ccggctgcat actctcaacg attatctggt agtcgccgcg cggctcgtag
    12601 agcgtaatat tggcgcgaac taaaacttgt tgcccatgct gcggacggaa ggtgacccgg
    12661 cggttgctgt tgcggaacat cgcgcagcgt acctgggcgg tgtcgtcttt gagtgtaaag
    12721 taccagtgac cggaagccgg ttgtgtgaaa ttagagattt cgccgctgat ccaaacctgt
    12781 cccatctcat gctcaagcag cagacgaacc gtttgattca ggcgactaac ggtaaaaatt
    12841 gcaggggatt gagaaggtaa catgtgagcg agatcaaatt ctaaatcagc aggttattca
    12901 gtcgatagta acccgccctt cggggatagc aagcattttt tgcaaaaagg ggtagatgca
    12961 atcggttacg ctctgtataa tgccgcggca atatttatta accactctgg tcgagatatt
    13021 gcccatgcta cgtatcgcta aagaagctct gacgtttgac gacgttctcc tcgttcctgc
    13081 tcattctacc gttctgccga atactgctga cctcagcacc cagctgacga aaactattcg
    13141 tctgaatatc cctatgcttt ccgcagcaat ggataccgta acggaagcgc gcctggctat
    13201 tgctctggct caggaaggcg gtatcggctt tatccacaaa aacatgtcca ttgaacgcca
    13261 ggcagaagaa gttcgccgtg tgaaaaaaca cgaatctggt gtggtgactg atccgcagac
    13321 tgttctgcca accacgacgc tgcgcgaagt gaaagaactg accgagcgta acggttttgc
    13381 gggctatccg gtcgttaccg aagaaaacga actggtgggt attatcaccg gtcgtgacgt
    13441 gcgttttgtt accgacctga accagccggt tagcgtttac atgacgccga aagagcgtct
    13501 ggtcaccgtg cgtgaaggtg aagcccgtga agtggtgctg gcaaaaatgc acgaaaaacg
    13561 cgttgaaaaa gcgctggtgg ttgatgacga attccacctg atcggcatga tcaccgtgaa
    13621 agacttccag aaagcggaac gtaaaccgaa cgcctgtaaa gacgagcaag gccgtctgcg
    13681 tgttggtgct gcagttggcg caggtgcggg taacgaagag cgtgttgacg cgctggttgc
    13741 cgctggcgtt gacgttctgc tgatcgactc ctcccacggt cactcagaag gcgttttgca
    13801 gcgtatccgt gaaacccgtg ctaaatatcc ggatctgcaa atcatcggcg gcaacgtggc
    13861 aacagctgca ggtgcacgcg ctttggcaga agctggttgc agcgcggtta aagtcggtat
    13921 cggccctggt tctatctgta cgactcgtat cgttactggc gtaggtgttc cgcagatcac
    13981 tgccgttgct gacgcagttg aagccctgga aggcaccggt attccggtta tcgcagatgg
    14041 tggtattcgc ttctccggcg acatcgccaa agctatcgcc gctggcgcaa gcgcggtgat
    14101 ggtaggttcc atgctggcgg gtactgaaga atctccgggt gaaatcgaac tctaccaggg
    14161 ccgttcttat aaatcttacc gtggtatggg ttccctgggc gcgatgtcca aaggttcctc
    14221 tgaccgttat ttccagagcg ataacgctgc cgacaaactg gtgccggaag gtatcgaagg
    14281 tcgcgtagct tataaaggtc gcctgaaaga gatcattcac cagcagatgg gcggcctgcg
    14341 ctcctgtatg ggtctgaccg gctgtggtac tatcgacgaa ctgcgtacta aagcggagtt
    14401 tgtacgtatc agcggtgcgg gcattcagga aagccacgtt cacgacgtga ccattactaa
    14461 agagtccccg aactaccgtc tgggctcctg attctcttcg cccgacttca tgtcgggcga
    14521 tttatattat ctgtttcact tgcctcggaa taagcgtcaa tgacggaaaa cattcataag
    14581 catcgcatcc tcattctgga cttcggttct cagtacactc aactggttgc gcgccgcgtg
    14641 cgtgagctgg gtgtttactg cgaactgtgg gcgtgggatg tgacagaagc acaaattcgt
    14701 gacttcaatc caagcggcat tattctttcc ggcggcccgg aaagtactac tgaagaaaac
    14761 agtccgcgtg cgccgcagta tgtctttgaa gcaggcgtac cgatattcgg cgtttgctat
    14821 ggcatgcaga ccatggcaat gcagttgggc ggtcacgttg aagcctctaa cgaacgtgaa
    14881 tttggctacg cgcaggtaga agtcgtaaac gacagcgcac tggttcgcgg tatcgaagat
    14941 gcgctgaccg cagacggtaa accgctgctc gatgtctgga tgagccacgg cgataaagtt
    15001 accgctatcc cgtccgactt cgtcaccgta gccagcaccg aaagctgccc gtttgccatt
    15061 atggctaacg aagaaaaacg cttctatggc gtacagttcc acccggaagt gactcacacc
    15121 cgccagggta tgcgcatgct ggagcgtttt gtgcgtgata tctgccagtg tgaagccctg
    15181 tggacgccag cgaaaattat cgacgatgct gtagctcgca tccgcgagca ggtaggcgac
    15241 gataaagtca tcctcggcct ctctggtggt gtggattcct ccgtaaccgc aatgctgctg
    15301 caccgtgcta tcggtaaaaa cctgacttgc gtattcgtcg acaacggcct gctgcgtctc
    15361 aacgaagcag agcaggttct ggatatgttt ggcgatcact ttggtcttaa cattgttcac
    15421 gtaccggcag aagatcgctt cctgtcagcg ctggctggcg aaaacgatcc ggaagcaaaa
    15481 cgtaaaatca tcgggcgcgt tttcgttgaa gtattcgatg aagaagcgca gaaactggaa
    15541 gacgtgaagt ggctggcgca gggcaccatc taccctgacg ttatcgaatc tgcggcttct
    15601 gcaaccggta aagcacacgt catcaaatct caccacaacg tgggcggcct gccgaaagag
    15661 atgaagatgg gcctggttga accgctgaaa gagctgttca aagacgaagt gcgtaagatt
    15721 ggtctggagc tgggcctgcc gtacgacatg ctgtaccgtc atccgttccc gggaccaggc
    15781 cttggcgttc gtgttctggg tgaagtgaag aaagagtact gtgacctgct gcgccgtgct
    15841 gacgccatct tcattgaaga actgcgtaaa gcggacctgt acgacaaagt cagccaggcg
    15901 ttcaccgtct tcctgccagt acgttccgtt ggcgtaatgg gcgatggtcg taagtatgac
    15961 tgggttgtct ctctgcgtgc tgtcgaaacc atcgacttta tgaccgcaca ctgggcgcat
    16021 ctgccgtacg atttcctcgg tcgcgtttcc aaccgcatta tcaatgaagt gaacggtatt
    16081 tcccgcgtgg tgtatgacat cagcggcaag ccgccagcta ccattgagtg ggaatgattt
    16141 tgatccctgc actatgaatg aacaaaaccc tccgttacta cagagggttt tttatcttca
    16201 agaatcatag gcttgaagtt actaacatcg attaattaaa ccagctgtcc gatttgttct
    16261 cttctgcttt gcccacgctt ttcatcagat cgcgaccgcc ttcagtcata tttctgtttg
    16321 cgtcagcttc agattgcacc acatcggttt gcgcagcttt gtgcttcagt tcctgatcga
    16381 taaattcgtt ttcgcgctta acgcgggctt cttctttcgc cagcgccagt ttttgtttct
    16441 gaatctctaa gctgcgtagc tcatcttcat aactttgatc gcgttttttg tccgcagtgg
    16501 cttcggcgtc cagtttatcc tgacgagctt tcttatttgc cgctgccgtt gccgctcttt
    16561 tgttcgcggc ggcctgggca tttgcgcgac gttgcttctc ttgctggatt tccctgttgc
    16621 gctccgcgac ccattcgtca tgctgccttt gctcttcatt tttaccttgc tgt
//
LOCUS       DAJTXY010000083        16484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTXY010000083 DAJTXY010000000
VERSION     DAJTXY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.083"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(52..2493)
                     /gene="rnr"
                     /locus_tag="OO566_002786"
     CDS             complement(52..2493)
                     /gene="rnr"
                     /locus_tag="OO566_002786"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6606007.1"
                     /translation="MSQDPFQGREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(2532..2957)
                     /gene="nsrR"
                     /locus_tag="OO566_002787"
     CDS             complement(2532..2957)
                     /gene="nsrR"
                     /locus_tag="OO566_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6606008.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(3162..4460)
                     /gene="purA"
                     /locus_tag="OO566_002788"
     CDS             complement(3162..4460)
                     /gene="purA"
                     /locus_tag="OO566_002788"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6606009.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4564..4761)
                     /locus_tag="OO566_002789"
     CDS             complement(4564..4761)
                     /locus_tag="OO566_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6606010.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4843..5847)
                     /gene="hflC"
                     /locus_tag="OO566_002790"
     CDS             complement(4843..5847)
                     /gene="hflC"
                     /locus_tag="OO566_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6606011.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5850..7109)
                     /gene="hflK"
                     /locus_tag="OO566_002791"
     CDS             complement(5850..7109)
                     /gene="hflK"
                     /locus_tag="OO566_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6606012.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7195..8475)
                     /gene="hflX"
                     /locus_tag="OO566_002792"
     CDS             complement(7195..8475)
                     /gene="hflX"
                     /locus_tag="OO566_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6606013.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8551..8859)
                     /gene="hfq"
                     /locus_tag="OO566_002793"
     CDS             complement(8551..8859)
                     /gene="hfq"
                     /locus_tag="OO566_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6606014.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8945..9895)
                     /gene="miaA"
                     /locus_tag="OO566_002794"
     CDS             complement(8945..9895)
                     /gene="miaA"
                     /locus_tag="OO566_002794"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6606015.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9888..11735)
                     /gene="mutL"
                     /locus_tag="OO566_002795"
     CDS             complement(9888..11735)
                     /gene="mutL"
                     /locus_tag="OO566_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6606016.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11745..13082)
                     /gene="amiB"
                     /locus_tag="OO566_002796"
     CDS             complement(11745..13082)
                     /gene="amiB"
                     /locus_tag="OO566_002796"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6606017.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13101..13562)
                     /gene="tsaE"
                     /locus_tag="OO566_002797"
     CDS             complement(13101..13562)
                     /gene="tsaE"
                     /locus_tag="OO566_002797"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6606018.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13534..15081)
                     /gene="nnr"
                     /locus_tag="OO566_002798"
     CDS             complement(13534..15081)
                     /gene="nnr"
                     /locus_tag="OO566_002798"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6606019.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15080..16219
                     /gene="queG"
                     /locus_tag="OO566_002799"
     CDS             15080..16219
                     /gene="queG"
                     /locus_tag="OO566_002799"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6606020.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16202..16255)
                     /locus_tag="OO566_002800"
     CDS             complement(16202..16255)
                     /locus_tag="OO566_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606021.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3740 a         4703 c         4140 g         3901 t
ORIGIN      
        1 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
       61 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
      121 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
      181 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac gacgcttgcc
      241 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
      301 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
      361 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
      421 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
      481 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
      541 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
      601 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
      661 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
      721 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
      781 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
      841 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
      901 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
      961 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1021 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1081 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     1141 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     1201 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     1261 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     1321 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     1381 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     1441 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     1501 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     1561 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     1621 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     1681 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     1741 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     1801 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     1861 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     1921 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     1981 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2041 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     2101 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     2161 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     2221 gccaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     2281 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     2341 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     2401 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     2461 agcttcgcgt ccctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     2521 gaagattttc gtcactccaa cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     2581 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     2641 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     2701 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     2761 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     2821 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     2881 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     2941 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3001 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3061 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     3121 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     3181 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     3241 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     3301 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     3361 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     3421 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     3481 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     3541 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     3601 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     3661 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     3721 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     3781 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     3841 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     3901 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     3961 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4021 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4081 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg
     4141 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     4201 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     4261 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     4321 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     4381 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     4441 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     4501 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgaaca gtctgttttt
     4561 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     4621 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     4681 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     4741 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     4801 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     4861 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     4921 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     4981 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5041 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     5101 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     5161 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     5221 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     5281 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     5341 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     5401 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     5461 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     5521 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     5581 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     5641 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     5701 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     5761 accacgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     5821 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     5881 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     5941 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6001 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6061 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     6121 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     6181 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     6241 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac
     6301 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     6361 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     6421 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     6481 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     6541 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     6601 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     6661 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     6721 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     6781 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     6841 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     6901 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     6961 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7021 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7081 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     7141 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     7201 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     7261 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     7321 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     7381 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     7441 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     7501 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     7561 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     7621 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     7681 tgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     7741 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     7801 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     7861 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     7921 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     7981 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8041 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     8101 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     8161 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     8221 gctcagggca tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat
     8281 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     8341 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     8401 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     8461 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     8521 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     8581 ggaagtattc tgtgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     8641 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     8701 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     8761 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     8821 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     8881 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     8941 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9001 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9061 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
     9121 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
     9181 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
     9241 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
     9301 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
     9361 ccgaaataaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
     9421 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
     9481 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
     9541 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
     9601 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
     9661 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
     9721 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aagggcagaa tcaacgctta
     9781 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
     9841 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
     9901 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
     9961 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10021 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10081 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    10141 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    10201 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    10261 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    10321 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    10381 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    10441 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    10501 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    10561 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    10621 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    10681 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    10741 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    10801 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    10861 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    10921 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    10981 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11041 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    11101 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    11161 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    11221 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    11281 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgccgccgg tttaaccgtc
    11341 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    11401 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    11461 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    11521 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    11581 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    11641 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    11701 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    11761 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    11821 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    11881 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    11941 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12001 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12061 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    12121 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgactg tttctcgtgc
    12181 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac caatacggaa
    12241 gcgccagtcg cactgcggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    12301 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    12361 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    12421 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    12481 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    12541 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    12601 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    12661 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    12721 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    12781 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    12841 agattattgc cgctgaacaa caacggcagt ccctgaatca cgcccgtttg tttgatatcg
    12901 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    12961 aacgttatcc gtgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13021 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13081 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    13141 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    13201 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccacaaggca gatggcatcg
    13261 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    13321 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    13381 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    13441 ttacctgcgc ctaaatcgcc atacagatag attacggttg ctccatcgca ggctttcgct
    13501 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    13561 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    13621 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    13681 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    13741 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    13801 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    13861 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    13921 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    13981 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14041 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    14101 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    14161 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    14221 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    14281 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    14341 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    14401 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    14461 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    14521 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    14581 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    14641 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    14701 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    14761 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    14821 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    14881 gcacagcacc agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    14941 ctcgccagcg cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg
    15001 ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt
    15061 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    15121 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    15181 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    15241 gatggcacgt cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg
    15301 tgtaatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    15361 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    15421 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    15481 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    15541 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt
    15601 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    15661 caaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    15721 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcga tcccggaaga
    15781 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc
    15841 gtggaatcgc tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca
    15901 cgcaccggaa ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga
    15961 aggatcggcg attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt
    16021 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca
    16081 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    16141 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    16201 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    16261 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac
    16321 ttatttatca ataggttaaa aaataccatt caccgtgcga agtttctttg aaattaagat
    16381 tacagcctga ggctgtggat aactctgttt acaagagttt gtcagagaca acaaaaacca
    16441 gcaataacgc atcgcgtcgc tgtggatatt ttattgagag aaga
//
LOCUS       DAJTXY010000084        16450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTXY010000084 DAJTXY010000000
VERSION     DAJTXY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.084"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..607
                     /locus_tag="OO566_002801"
     CDS             <1..607
                     /locus_tag="OO566_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6606022.1"
                     /translation="AQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTH
                     PIDLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTI
                     KVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVS
                     KHLVTPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
     gene            607..3729
                     /gene="mdtB"
                     /locus_tag="OO566_002802"
     CDS             607..3729
                     /gene="mdtB"
                     /locus_tag="OO566_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR6606023.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLVLWTK
                     SRFARHEEEA"
     gene            3730..6795
                     /gene="mdtC"
                     /locus_tag="OO566_002803"
     CDS             3730..6795
                     /gene="mdtC"
                     /locus_tag="OO566_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6606024.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLRITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            6796..8211
                     /locus_tag="OO566_002804"
     CDS             6796..8211
                     /locus_tag="OO566_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6606025.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            8208..9611
                     /gene="baeS"
                     /locus_tag="OO566_002805"
     CDS             8208..9611
                     /gene="baeS"
                     /locus_tag="OO566_002805"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6606026.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            9608..10330
                     /gene="baeR"
                     /locus_tag="OO566_002806"
     CDS             9608..10330
                     /gene="baeR"
                     /locus_tag="OO566_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6606027.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            10521..10853
                     /locus_tag="OO566_002807"
     CDS             10521..10853
                     /locus_tag="OO566_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6606028.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            11000..12361
                     /gene="yegQ"
                     /locus_tag="OO566_002808"
     CDS             11000..12361
                     /gene="yegQ"
                     /locus_tag="OO566_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6606029.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(12703..13020)
                     /locus_tag="OO566_002809"
     CDS             complement(12703..13020)
                     /locus_tag="OO566_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606030.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            13426..14325
                     /gene="yegS"
                     /locus_tag="OO566_002810"
     CDS             13426..14325
                     /gene="yegS"
                     /locus_tag="OO566_002810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6606031.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(14407..15180)
                     /locus_tag="OO566_002811"
     CDS             complement(14407..15180)
                     /locus_tag="OO566_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6606032.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(15286..16326)
                     /gene="gatD"
                     /locus_tag="OO566_002812"
     CDS             complement(15286..16326)
                     /gene="gatD"
                     /locus_tag="OO566_002812"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6606033.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
BASE COUNT         3793 a         4395 c         4310 g         3952 t
ORIGIN      
        1 cgcgcagttg caactcgact ggagccgcat caccgcacca gtcgatggtc gcgttggtct
       61 caagcaagtt gatgttggta accaaatctc cagtggtgat accaccggaa ttgtggtgat
      121 cacccagacg catcctatcg atttagtctt taccctgccg gaaagcgata tcgctactgt
      181 agtgcaggcg caaaaagccg gaaaaccgct gatggtagaa gcctgggatc gcaccaattc
      241 gaagaaatta agtgaaggca cgctgttaag cctcgataac caaatcgatg ccacaaccgg
      301 tacgattaaa gtgaaagcac gctttaataa tcaggatgat gcgctgtttc ccaatcagtt
      361 tgttaacgcg cgcatgttag tcgacaccga acaaaacgcc gtagtgatcc caacagccgc
      421 cctgcaaatg ggcaatgaag gccattttgt ctgggtgctg aatagcgaaa acaaggtcag
      481 caaacatctg gtgacgccgg gcattcagga cagtcagaaa gtggaagtgg tggaagccca
      541 gagcgccacc actccggaag agaaagccac cagccgcgaa tacgcgaaaa aaggagcacg
      601 ctcctgatgc aggtgttacc cccgagcagc acaggcggcc cgtcgcgcct gtttattatg
      661 cgtcctgtcg ccaccacgct gctgatggtg gcgatcttac tcgccgggat tatcggttat
      721 cgcgccctgc ccgtttcggc gctgccggaa gtggactatc cgaccattca ggtggtcacg
      781 ctctacccag gtgccagccc ggatgtcatg acctctgccg ttaccgcgcc gctcgaacgc
      841 cagttcgggc agatgtctgg cctgaaacag atgtcgtcgc aaagttccgg cggtgcgtca
      901 gttatcactt tgcagttcca gctaacatta ccgctcaatg tcgccgagca ggaagtgcag
      961 gccgcgatta acgctgcgac caacttgttg ccgagcgatc tgcctaaccc gccggtttac
     1021 agcaaagtga acccggcaga tccgccgatc atgacgctcg ccgtcacctc aaccgccatg
     1081 ccgatgacgc aagtggaaga tatggtggaa acccgcgtcg cgcagaaaat ctcgcagatt
     1141 tccggcgtcg gcctggtgac gctttccggc ggtcagcgtc cggctgttcg cgtcaaactt
     1201 aacgctcagg cgattgccgc cctcggcctg accagcgaaa ccgtgcgcac cgccattacc
     1261 ggcgctaacg ttaactcggc aaaaggtagc ctcgacggcc cttcccgtgc ggtcacgctt
     1321 tccgcgaacg accagatgca atccgccgaa gagtatcgcc agctaatcat cgcctaccag
     1381 aacggcgcgc caattcgtct gggcgatgta gcaactgtag agcaaggtgc agaaaacagc
     1441 tggctcggcg cgtgggcgaa caaagaacag gccattgtga tgaatgttca gcgccagccc
     1501 ggtgctaaca ttatctccac cgccgacagc attcggcaga tgctgccaca gctcactgag
     1561 agtctgccga aatcggtgaa ggtgacagtg ctttccgatc gcaccaccaa tatccgcgca
     1621 tccgtcgatg atactcagtt tgaattgatg atggctatcg cgctggtagt catgattatc
     1681 tacctgtttt tgcgcaatat tccggcgacc atcattcccg gtgttgctgt accgctgtcg
     1741 ttaatcggca ctttcgcggt tatggtgttt ctcgattttt caatcaataa cctgacactg
     1801 atggcgttaa ctatcgccac cggattcgtg gtcgatgacg ccatcgtggt gatcgaaaac
     1861 atttcccgct atatcgaaaa aggcgaaaaa ccgttggcgg cggcgctcaa gggcgcaggt
     1921 gaaatcggct ttaccattat ctcgctgacc ttctcactga ttgcggtgtt gatcccactg
     1981 ctgtttatgg gcgatatcgt cgggcgactg ttccgcgaat ttgctattac cctggcggta
     2041 gcgattttga tctcagcggt ggtgtcgctg accctgacac cgatgatgtg cgcgcggatg
     2101 ctcagccagg agtcgttgcg taaacagaac cgcttctccc gtgcctcgga aaaaatgttc
     2161 gacaggataa tcgccgccta tggtcgtgga ctggcgaaag tgctgaatca tccgtggctg
     2221 accttaagcg tggcactcag cacgctgctg cttagcgtgc tgctgtgggt gttcattccg
     2281 aaaggtttct tcccggtaca ggacaacggc attattcagg gcactttgca ggcaccgcaa
     2341 tccagctcct ttgccaatat ggcccagcga caacgccagg tcgcggacgt gattttgcag
     2401 gatccggcag tgcaaagcct gacctcattt gttggcgttg atggcactaa cccgtcgctg
     2461 aatagtgcac gtttacaaat caacctcaaa ccgttggatg aacgtgatga tcgggtgcaa
     2521 aaagtcatcg cccgtctgca aacggcggta gataaagtgc cgggcgtcga tctcttcctg
     2581 caaccaacgc aggacctgac catcgatact caggtcagcc gcacccagta ccagtttacc
     2641 ttgcaggcca cgtcactgga tgcgctcagt acctgggtgc cacagttgat ggaaaaactc
     2701 cagcaactgc cacagctttc tgatgtctcc agcgactggc aggacaaagg gctggtggcg
     2761 tatgtcaatg ttgatcgcga cagcgccagc cgtctgggga tcagcatggc ggacgtcgat
     2821 aacgccctgt acaacgcgtt tggtcagcga ctgatttcca ctatttatac tcaggctaac
     2881 cagtatcgcg tggtgctgga gcacaatacc gaaaataccc caggcctcgc ggcgctggat
     2941 accattcgcc tgaccagcag cgacggtggc gtggtgccgt taagctcaat tgccaaaatt
     3001 gagcagcgtt ttgcgccgct ttccatcaac catctggatc agttcccggt aacaaccatc
     3061 tcctttaacg tgccggataa ctattcgctg ggcgatgcgg tgcaggcgat tatggacacc
     3121 gaaaagacgc tgaatctgcc ggtggatatc accacgcaat tccaaggcag caccctcgcc
     3181 ttccagtcgg cgctgggcag cactgtctgg ctgattgtcg cggcggtggt ggcgatgtat
     3241 atcgtgctcg gcattctgta cgagagcttt attcacccga tcaccattct ctcgacgcta
     3301 cccaccgcag gggtcggcgc gctgctggca ttaatgattg ccggtagcga actggatgtg
     3361 attgcgatta tcggcattat tttgctgatc ggtattgtga agaagaacgc catcatgatg
     3421 atcgacttcg cgctggctgc tgagcgcgag caaggcatgt cgccgcgcga tgcaatctac
     3481 caggcttgtc tgttgcgttt tcgtccgatc ctgatgacca ccctggcggc tctgcttggc
     3541 gcgctgccgc tgatgttgag taccggggtc ggcgcggaac tgcgtcgtcc gttaggtatc
     3601 ggcatggtcg gcggtctgat tgtcagccag gtgctgacgc tgttcaccac gccggtgatt
     3661 tatctgctgt tcgaccgcct ggtattgtgg accaaaagtc gctttgctcg ccatgaagag
     3721 gaggcgtaag tgaagttttt tgccctcttc atttaccgcc cggtggcgac gattttactg
     3781 tcggtcgcca ttaccctgtg cggcatactg ggtttccgta tgctgccggt cgccccgctg
     3841 ccgcaggtcg attttccggt gattatggtc agcgcctcgc tgcccggtgc gtcgccggag
     3901 acaatggcgt cttccgttgc cacgccgctg gagcgctcac ttgggcgcat tgccggggtt
     3961 agtgaaatga cctccagcag ttcgctcggc agcacgcgca ttattttgca gtttgatttt
     4021 gaccgggata tcaacggcgc agcgcgtgat gtgcaggcgg cgatcaacgc tgcacaaagt
     4081 ttgctgccca gcgggatgcc cagccgcccg acctatcgca aagcgaaccc gtcggatgcg
     4141 ccaattatga tcctcacgct gacatccgat acttattcgc agggtgaact gtacgatttc
     4201 gcctcgacgc agctggctcc gacgatttcg caaatcgacg gtgtcggtga tgtcgatgtc
     4261 ggtggcagct cgctgcccgc cgtgcgcgtc gggctgaacc cgcaggcgct gtttaatcag
     4321 ggcgtgtcgc tggacgacgt acgcactgcc atcagcaatg ccaacgtgcg taaaccgcag
     4381 ggggcgctgg aagatggcac tcaccgctgg cagatccaga ccaacgatga actgaaaacc
     4441 gccgctgaat atcagccgct gattattcac tacaacaacg gcggcgcggt tcgtctgggc
     4501 gatgtggcga cggtgactga ctcagtgcag gatgtgcgca acgccgggat gaccaacgcc
     4561 aaaccggcta ttttactgat gatccgcaaa ctgccggaag ccaatattat ccagacggta
     4621 gacagcatcc gggcaaaatt accagagttg caggagacca ttccggcggc gattgatctg
     4681 caaattgccc aggatcgctc ccccaccatt cgcgcctcgc tggaagaagt cgagcaaacg
     4741 ctgattatct cggtggcgct ggtgattctg gtggtctttt tattcctgcg ctcgggtcgc
     4801 gccactatta ttcccgccgt tgcggtgccg gtttcgctga ttggtacgtt tgcggcgatg
     4861 tacctgtgcg gattcagtct caataatctt tcgctgatgg cgctcaccat tgctactggt
     4921 ttcgtggtgg atgacgccat tgtggtgctg gaaaacattg cccgtcatct ggaagcgggg
     4981 atgaaaccac tacaggccgc gctgcaaggt actcgcgaag tcggctttac ggtgctgtcg
     5041 atgagtctgt cactggtggc ggtgttcctg ccgctgctgt tgatgggcgg attgccgggc
     5101 cgactgttac gcgagtttgc cgtgacgctt tctgtcgcca ttggtatttc gttgctggtt
     5161 tccctgacgc taacgccaat gatgtgcggc tggatgctaa aagccagcaa gccgcgtgag
     5221 caaaagcgac tgcgtggttt tggccgcatg ttggtagccc tgcaacaagg ctacggcaag
     5281 tcgctgaaat gggtgctcaa tcatacccgt ctggtgggcg tggtgctgct tggcaccatt
     5341 gcgctgaata tctcgatccc gaaaaccttc ttcccggagc aggacaccgg cgtactgatg
     5401 ggtgggatac aggcggatca gagtatttcg tttcaggcga tgcgcggcaa attgcaggat
     5461 ttcatgaaaa ttatccgtga cgatccggca gtggataatg tcaccggctt taccggcggt
     5521 tcacgagtta acagtgggat gatgtttatc accctcaagc cacgcgacga acgcagcgaa
     5581 acggcgcagc aaattatcga ccgtctgcgc gtgaagctgg cgaaagaacc gggggcgaat
     5641 ctgttcctga tggcggtaca agatattcgc gttggtgggc gtcagtcgaa cgccagctac
     5701 cagtacacgt tgttatccga cgacctggcg gcactgcgcg aatgggagcc gaaaatccgc
     5761 aaaaaactgg cgacgttgcc ggaactggcg gacgtgaact ccgatcagca ggataacggc
     5821 gcggagatga atctggttta cgaccgcgac accatggcac ggctgggaat cgacgtacag
     5881 gccgccaaca gtctgttaaa taacgccttc ggtcagcggc aaatctcgac catttaccag
     5941 ccgatgaacc agtataaagt ggtgatggaa gtggatccgc gctataccca ggacatcagt
     6001 gcgctggaaa aaatgttcgt tatcaataac gaaggcaaag cgatcccgct gtcgtatttc
     6061 gctaaatggc aaccggcgaa tgccccacta tcggtgaatc atcagggatt atcggcggcc
     6121 tcgaccatct cgttcaacct gccgaccgga aaatcgctct cggacgccag tgcggcgatc
     6181 gatcgcgcaa tgacccagct tggtgtgcct tcgacggtgc gcggcagttt tgccggcacg
     6241 gcgcaggttt tccaggagac gatgaactcg caggtgatcc tgattatcgc cgccatcgcc
     6301 acggtgtata tcgtgctggg tatcctttac gagagttacg tacatccgct gacgattctc
     6361 tccaccctgc cctcggcggg cgttggagcg ctgttggcgc tggagctgtt caatgccccg
     6421 ttcagcctaa tcgccctgat agggatcatg ctattaatcg gcatcgtgaa gaaaaacgcc
     6481 attatgatgg tcgattttgc gcttgaagcc caacggcacg gtaacctgac gccgcaggaa
     6541 gctattttcc aggcctgtct gctgcgtttt cgcccgatta tgatgaccac cctggcggcg
     6601 ctgtttggcg cgctgccgct ggtattgtcg ggcggtgacg gctcggagct gcggcaaccc
     6661 ctgaggatca ccattgtcgg cggtctggtg atgagccagc tcctgacgct gtataccacg
     6721 ccggtggtgt atctcttttt cgaccgtctg cggctgcgtt tttcgcgtaa acctaaacaa
     6781 acggtaaccg agtaaatgac agatcttccc gacagcaccc gctggcaatt atggattgtg
     6841 gctttcggct tctttatgca gtcgctggac accaccatcg taaacaccgc ccttccctca
     6901 atggcgcaaa gcctcgggga aagtccgttg catatgcaca tggtcattgt ctcttatgtg
     6961 ctgaccgtgg cggtgatgct gcccgccagc ggctggctgg cggacaaagt cggcgtgcgc
     7021 aatattttct ttaccgccat cgtgctgttt actctcggtt cactgttttg cgcgctttcc
     7081 ggcacgctga acgaactgtt gctggcacgc gcgttacagg gcgttggcgg tgcgatgatg
     7141 gtgccggtcg gcaggttgac ggtgatgaaa atcgtaccgc gcgagcaata tatggcggcg
     7201 atgacctttg tcacgttacc cggtcaggtc ggtccgctgc tcggtccggc gctcggcggt
     7261 ctgctggtag aatacgcgtc gtggcactgg atctttttga tcaacattcc ggtggggatt
     7321 atcggtgcga tcgccacatt gatgttaatg ccgaactaca ccatgcagac gcggcgcttt
     7381 gatctttccg gctttttatt gctggcggtt ggcatggcgg tattaaccct ggcgctggac
     7441 ggcagtaaag gtacaggttt atcgccgctg gcgattgctg gcctggtcgc agttggcgtg
     7501 gtggcactgg tgctttatct gctgcacgcc agaaataaca accgcgccct gttcagtctg
     7561 aaactgttcc gcactcgtac cttttcgctg ggcctggcgg ggagctttgc cggacgtatt
     7621 ggcagtggca tgttgccctt tatgacaccg gttttcctgc aaattggcct cggtttctcg
     7681 ccgtttcatg ccggactgat gatgatcccg atggtgcttg gcagcatggg aatgaagcga
     7741 attgtggtac aggtggtgaa tcgctttggt tatcgtcggg tactggtagc gaccacgctg
     7801 ggtctgtcgc tggtcaccct gttgtttatg actaccgctc tgttgggctg gtactacgtt
     7861 ttgccgttcg tcctgttttt acaagggatg gtcaactcga cccgtttctc ctccatgaac
     7921 accctgacgc tgaaagatct cccggacaat ctggcgagca gcggaaacag cctgctgtcg
     7981 atgattatgc aattgtcgat gagtatcggc gtcaccatcg ccgggctgtt gctggggctt
     8041 tttggttcgc agcatgtcag cgtcgacagc ggcaccacac aaaccgtctt tatgtacacc
     8101 tggcttagca tggcgtttat catcgccctt ccggcgttca tctttgccag agtgccgaac
     8161 gatacgcatc aaaatgtagc tatttcgcgg cgaaaaagga gcgcgcaatg aagttctggc
     8221 gacccggtat taccggcaaa ctgtttctgg cgattttcgc cacctgcatt gtcttgctga
     8281 tcagtatgca ctgggcggtg cgtatcagtt ttgagcgtgg ctttattgat tacatcaaac
     8341 acggcaatga acagcgatta cagctgttaa gtgatgcgct tggcgagcag tatgcgcagc
     8401 atggtaactg gcgcttcctg cgcaacaacg atcgctttgt ctttcagatc ctgcgttcat
     8461 ttgaacacga taattcggaa gataaacccg gcccgggtat gccgccgcac ggctggcgta
     8521 cccagttctg ggtggtcgat caaaacaaca aagtactggt tggtccgcga gcgccgattc
     8581 cacctgacgg tacacggcga cccattctgg tcaacggtgc ggaagtgggc gcggtgattg
     8641 cctcccctgt tgagcggctg acccgcaata ctgatatcaa tttcgataaa caacagcggc
     8701 aaaccagctg gctgatcgtc gccctggcaa cgttactcgc ggcgctcgcc acctttctgc
     8761 tggcgcgcgg tttgctggca ccggtaaaac gacttatcga tggcacgcac aaactggcgg
     8821 cgggcgattt cactacccgc gtaacgccca ccagtgaaga tgaactgggc aaactggcgc
     8881 aagacttcaa ccagctcgcc agcacgctgg agaaaaacca gcaaatgcgg cgcgatttta
     8941 tggccgatat ttctcacgaa ctgcgtacgc cattagcggt gctgcgcggt gaactggaag
     9001 ccattcagga tggcgtgcgc aaattcacgc cggagacggt ggcgtcttta caggcggagg
     9061 tcggtacact gaccaaactg gttgacgatc ttcatcagtt gtcgatgtct gatgaaggcg
     9121 ctctcgccta tcaaaaagca ccggtagatt tgatcccact gctggaagtg gcgggcggcg
     9181 catttcgcga acgattcgcc agccgtggcc tgaaactgca attttccctg ccagacagta
     9241 tcaccgtatt tggcgatcgc gaccgtttaa tgcagttatt caataactta ctggaaaaca
     9301 gcctgcgcta cactgacagc ggcggcagcc tgaaaatctc tgccgagcag cacgacaaaa
     9361 cggtgcgcct gacctttgcc gacagtgcgc caggtgtcag tgacgatcag ctacaaaaat
     9421 tgtttgaacg tttttatcgc accgaaggtt cccgcaaccg cgccagcggc ggttccgggc
     9481 tggggctggc gatttgcctg aacattgttg aagcccataa tggccgcatt attgccgccc
     9541 attcgccttt tggcggggta agcattacag tagagttacc gctggaacgg gatttacaga
     9601 gagaagtatg accgagttac caatcgacga aaacacaccg cgtattttga tcgtggaaga
     9661 tgaaccgaag ctggggcagt tgctcattga ttatctgcgt gctgcgagct atgcgccgac
     9721 gcttatcagc cacggcgatc aggtactggc gtatgtgcgc cagacaccac cggatctgat
     9781 cctgttggat ctgatgctcc ctggcaccga tggcctgaca ttatgccggg aaattcgtcg
     9841 tttttccgat gttccgatcg tgatggtgac ggcaaaaatc gaagagatcg accgcctgct
     9901 ggggctggag attggcgccg atgactacat ctgcaagccg tacagcccac gggaagtggt
     9961 agcgcgtgtc aaaaccattt tgcgccgttg taaaccgcaa cgcgaacttc agcaacagga
    10021 tgccgaaagc ccgttgatta tcgacgaagg tcgttttcag gcttcatggc gcggtaaaat
    10081 gcttgacctg acgcctgcgg aatttcgtct gcttaaaacg ctctctcacg aaccaggaaa
    10141 agtgttctcc cgcgagcaat tgctcaatca tctttatgac gactaccgcg tagtaaccga
    10201 ccgcaccatt gacagccaca ttaaaaatct gcgccgcaag ctggaatctc tcgacgccga
    10261 acagtcattt atccgcgccg tttacggcgt tggttaccgc tgggaagccg acgcctgccg
    10321 catcgtttag ttttagcgac attactttgt tagccggaga tggcgttcag acccgatccg
    10381 gcaacattat tccacgcatg gtcagcaaac tgacattatc tctccgccct ggcaggcatc
    10441 agaaaatcgc tcatacttta atcagtaaac agcaccttta gatgctgttt tcgataagca
    10501 atttcaatca aggagtcatt atggctggtt ggtttgaact cagtaagagc agtgataatc
    10561 agttccggtt tgtgctaaaa gcgggcaatg gtgagactat cctcaccagc gagctgtata
    10621 cctcaaaagc ctctgcggaa aagggcatcg cgtcggtgcg tagcaacagc ccgcaagaag
    10681 agcgctatga gaaaaaaacg gcaagcaacg gcaaattcta tttcaatctg aaagccgcta
    10741 atcatcaaat tatcggctcc agccagatgt acgccaccgc gcaatctcgt gaaaccggaa
    10801 ttgcctccgt taaagccaat ggcacaagcc agacggtgaa agacaatacg taattacgat
    10861 gccgggcgtg ggttgttggc gtccggcgct ttaccagatc ccccctacag cgctacaatg
    10921 cccgccctta aagtgggggc ctcccctaac cgcttcatca ggtgaagcgg atctgacctg
    10981 tcatcagaac gagagaatta tgtttaaacc ggaactcctt tccccggcgg gaacgctgaa
    11041 aaatatgcgt tacgctttcg cttatggcgc agatgctgtt tatgcgggcc agccgcgtta
    11101 ctccctacgt gtgcgcaaca acgaattcaa ccacgaaaat cttcagctcg gcatcaatga
    11161 agcccacgcg ctggggaaaa agttttatgt cgtggtcaac attgcaccac acaacgccaa
    11221 gctgaaaacc tttatccgtg acctgaaacc ggtggtggaa atggggccgg atgcgctgat
    11281 tatgtccgat ccagggctga ttatgctggt gcgtgagcac ttcccagaaa tgccgatcca
    11341 cctctcggtg caggctaacg ccgtgaactg ggcgacggtg aaattctggc agcaaatggg
    11401 actgacccgc gtgatcctct ctcgcgagct gtcgctggaa gagattgaag agatccataa
    11461 tcaggtgccg gatatggaga tcgagatctt cgttcacggc gcactgtgca tggcctactc
    11521 cggtcgctgc ctgctctctg gctatatcaa caagcgcgac ccgaaccagg gcacctgcac
    11581 caacgcctgc cgctgggagt acaacgtcca ggaagggaaa gaagatgacg ttggcaacat
    11641 cgtacacaag tacgagccga ttccggtgca aaatgttgag ccgacgctgg gtatcggcgc
    11701 gccaaccgac aaagtgttta tgatcgaaga agcccagcgt ccgggcgagt atatgaccgc
    11761 gtttgaagat gagcacggca cttacatcat gaactcgaaa gatctgcgcg ccatcgccca
    11821 tgtagaacgc ctgaccaaaa tgggcgtgca ttcgctgaaa atcgaaggtc gtaccaaatc
    11881 tttctactat tgcgcacgca ccgcacaggt ttaccgcaaa gctatcgatg acgccgctgc
    11941 gggaaaaccg ttcgatacca gcctgctgga aactctggaa ggtctggcgc atcgtggcta
    12001 taccgaaggt ttcctgcgtc gtcatactca cgacgattat cagaactacg aatacggtta
    12061 ttcagtttct gaccgccagc agtttgttgg tgagtttacc ggtgagcgca agggggacct
    12121 cgcagcggta gcggtgaaaa ataaattctc cgttggcgac agtcttgagc tgatgacgcc
    12181 gcaaggcaac attaacttta cccttgagca catggaaaac gccaaaggcg aagctatgcc
    12241 ggtcgcacca ggcgatggtt atactgtgtg gctcccggtc ccgcaggatc ttgagctaaa
    12301 ttacgcgctg ctaatgcgta atttctccgg ggaaaccacg cgtaaccccc acggtaagtg
    12361 attaatttcg attatttttc ccggatggaa aattcttaga aaccgatcac atacagctgc
    12421 atttattaag gttatcatcc gtttcgctga aaaacataat ccataaaatg ctagctgtac
    12481 caggaaccac ctccttagcc tgtgtaatct cccttacacg ggcttatttt ttgtacataa
    12541 gctgtaaaga aggattgatc cctgataacc gtcttccatt gaccacatcg atagaatccc
    12601 ccttcatagc acaatgcctt tcacgtaacg gtatcgtgct cgcccaggtt ccggctacgc
    12661 acagccagaa cgcgcatgtt tgacgcttac caaaaaatat tctcactctc cacatttgaa
    12721 tgtcagacga gcgacgccat gtaatcctgc accttctgtc ttcaggtcaa ctatctgcat
    12781 ttttttgccc tgagtaacac agaaatgggc tgcatcattt tttactatat tttctgcacc
    12841 agatattctg cccctggcta aagaagcttc ggcttcggtg tagtattggt tatcgagttt
    12901 acgctgaata ttacttttat atgcaagacc aaatttaccg atacttgttt catcattatg
    12961 cacagcacaa ccagatataa gaaaaatact aattaatgat atagcagcta tctttttcat
    13021 ctcaccttcc cccattaaat accaacgaca ctctctagtg tttaaatata ataatggcat
    13081 gattattata attgaatagg attataataa atgttctgta caacatttcc tacataagta
    13141 ggaattacgg acattgaggc ccttcagggt aactccatgg gggctttaat atattatatt
    13201 gaagatgcca ctgtttagtt gaatattagg tatatgctct tttttgaaat ttataggtgg
    13261 cagccgttag tattcgctgt ccccattgca agctcctggt ggtaaccact gaatcctcca
    13321 tacttgaact gactttttat cctccgactt tcatcctgtt ctgactccac cttttgtttt
    13381 ctgctctaca ctatctacag accaatcata aaggcacata cgatcatggc agaatttccc
    13441 gccagcttac tgattcttaa tggcaaaagt actgacaatc tacccttgcg cgaagcaatt
    13501 atgctgttgc gtgaggaagg aatgacgatc catgtgcggg tcacctggga gaaaggtgat
    13561 gccgcacgat ttgtagagga ggcccggaag ttgggcgtcg caacggtgat tgccggtggt
    13621 ggcgatggca ccattaatga agtttctacg gcgttgattc agtgtgaggg ggatgacata
    13681 cccgcgctgg gaattttgcc attaggaacc gccaatgatt ttgccaccag tgtagggatt
    13741 cctgaggcac tggataaggc gctgaaactg gcaattgccg gtaatgccat tgcgatagat
    13801 atggcgcagg tcaacaaaca aacctgtttt attaatatgg cgacaggcgg atttgggacg
    13861 cgtattacca cagaaacgcc ggaaaaatta aaagccgcgc tgggtggcgt ctcttacatc
    13921 attcatggct taatgcgcat ggatactctg caaccggacc gttgtgaaat ccgcggtgaa
    13981 aactttcact ggcaaggtga cgccctggtc attggtattg gtaacgggcg tcaggccggt
    14041 ggcggtcaac aattgtgccc gaacgcgtta attaacgatg gcttgctgca actgcgcatt
    14101 tttaccggcg atgaaattct tccggctctc gtatcaacct taaaatctga cgaagataac
    14161 ccgaatatta tcgaaggcgc ttcgtcgtgg tttgatatac aagccccaca cgaaatcact
    14221 tttaatcttg atggcgaacc gttgagtggg caaaacttcc atattgaaat acttccggcg
    14281 gcgttgcgtt gtcgattacc accagattgt ccattattgc gttaatcaaa ctattctctc
    14341 ttgaaccagg gagaatccgt aatgccggat gcggtgtaaa caccttatcc ggcatacaga
    14401 acaatactac accgtaatta attttacccg tgtcttctgc aatgccaatt tatccacctc
    14461 tgacacagca tcatcggtaa caataatgtc aattttttcg attggcagta cctgattaaa
    14521 accgcgacga ttaaatttcg atgaatcgag aacggcaatc actttatttg cggcggtgac
    14581 catcgccccg ctaatcgaat aaccttcatt aaaggtcgta ataccattaa ccgcatcaat
    14641 accatcagca ccgacaaaca ttaaatcggc attaatatcc tgcaatgaac gctcggcaat
    14701 ggaaccatgc atcgagcgcg ttttatggcg gaccgtgcca ccacagacga ccagagtaat
    14761 gtctttattt tcggaaaggg caaacgctgc cgggagactg ttggtaatca ccgtgatatt
    14821 tttagcggtc attaatcctt cggcgataag cattgtggta cttccgctgt cgagaataac
    14881 ggtcatccct tcatggatca ttttgactgc cgcctgcgct attctgtttt ttggcgcgct
    14941 ggcgagctga aatcgctctt taaacccgac ttcctgggtc tcggtttcac tattaccgga
    15001 cattattttc gccgcaccgc catgaaagcg cgtaaccacg cctttttgtt cgagaaagcg
    15061 caaatcggca cggattgtcg cttccgaggc agcaaatact cccgccagat cctgaacaag
    15121 cacggttccc tgttcattca ctaattggat gatcttattc cttcgctcga atgaattcat
    15181 cgtcatttac ccatattcaa ttgtggctag tgtaaacgaa gggggaccat tggtgaatga
    15241 tctgttttgc acaataccgg gccagcatca cgctggcccg cggtttcatg gaatgagcaa
    15301 cactttgccc ggcatggcat tacgagcgat gtcacgcacc gcctgggtga agctttcaaa
    15361 gcttccacgg tgagcgatta atggatccag gcttaactta cgttctgtca gcaaccggct
    15421 cgccgtttcc cactcctgcc ccggccaagg gctggaatag ttcatccaac tgccgataac
    15481 cgtcagctct ttacgcaata ttttgccaaa cgttgccgat gttaaatgca gatcctgatg
    15541 caacgtgccc accagcgcca gttgggcatg aggaccggca atctctaccg ccagttcgac
    15601 agtttgcggt acgccagccg tctcgaggat aagctgatta aagcgcagtt cgcgtaaaac
    15661 gctctgcatt tgcggcgcgc tcatttcgct actgttaaat gtttgcatcg caccgaaaga
    15721 ttttgccagt gccagttttt ctgagctaat gtcgatcgcc gtcacactct ttgctcccag
    15781 cgcgacagcg cactgaatag ccagcaggcc aatggttccg gcaccaataa taataacgtt
    15841 tttattctca caaccttgcg ctaaatgaaa agcatgcagg ccaacggtaa tcggctcaat
    15901 aaaagcccca tcctcaatag gcatatccgt gggtagagca aagacatttt ttcgcttaac
    15961 gacaatatat tcagcaaatc caccatcacg ccgcgagcca ataaaatcat attttgcgca
    16021 ctgggaataa aaccctttca aacactctgg acaagtaaaa cagggtaata acggcacaca
    16081 ggcaaccgca tcgccagggt gtaaatcatc aacaccggat cccaccgcat caatatagcc
    16141 gctaaattca tggcctaacg ttattggata ataatgtgca ccatttttaa atatcctggg
    16201 taaatcggaa ccacataagc ccgagctggc aatttttacc cgcacctcat cctgatgttt
    16261 aatttcagga atgacgcttt ttgcaacgcg cacgatacca tcagtatcat tcactactga
    16321 tttcataaaa actcctgatt gttaaggggg ataacccccc cctcaggtaa aaattattct
    16381 gcgagaacgg ctttctcttg tttagtaaaa ccacgcgctc tacgccaggt catgaaaata
    16441 ccggtcagat
//
LOCUS       DAJTXY010000085        16338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTXY010000085 DAJTXY010000000
VERSION     DAJTXY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.085"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(54..1493)
                     /gene="rsmF"
                     /locus_tag="OO566_002813"
     CDS             complement(54..1493)
                     /gene="rsmF"
                     /locus_tag="OO566_002813"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6606034.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(1573..4071)
                     /gene="yebT"
                     /locus_tag="OO566_002814"
     CDS             complement(1573..4071)
                     /gene="yebT"
                     /locus_tag="OO566_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR6606035.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTITIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLMTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKV
                     TGEMTVDPSVVTLLRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVV
                     VPGEKALLQEPDVLTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAI
                     EPQHRELVKGDSKFVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASY
                     PLYANLEKALENSLSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAF
                     DIDLHIKPEYRNLLTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGA
                     SASQRKGDKRILYASETAARAVGRQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLI
                     TARNEVQAKAVLYPEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGN
                     PRRDFELEEATITDSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLS
                     DRVMIAMRISKRYQHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATP
                     PGTPLAPKAQEGKHFLLQESEPKEWREWGTALPK"
     gene            complement(4040..5324)
                     /gene="yebS"
                     /locus_tag="OO566_002815"
                     /pseudo
     CDS             complement(4040..5324)
                     /gene="yebS"
                     /locus_tag="OO566_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            5454..5951
                     /gene="msrC"
                     /locus_tag="OO566_002816"
     CDS             5454..5951
                     /gene="msrC"
                     /locus_tag="OO566_002816"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6606036.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6048..6746
                     /gene="proQ"
                     /locus_tag="OO566_002817"
     CDS             6048..6746
                     /gene="proQ"
                     /locus_tag="OO566_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6606037.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            6766..8814
                     /gene="prc"
                     /locus_tag="OO566_002818"
     CDS             6766..8814
                     /gene="prc"
                     /locus_tag="OO566_002818"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6606038.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9006..9887
                     /gene="htpX"
                     /locus_tag="OO566_002819"
     CDS             9006..9887
                     /gene="htpX"
                     /locus_tag="OO566_002819"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6606039.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(9933..11306)
                     /locus_tag="OO566_002820"
     CDS             complement(9933..11306)
                     /locus_tag="OO566_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6606040.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            11483..12274
                     /gene="kdgR"
                     /locus_tag="OO566_002821"
     CDS             11483..12274
                     /gene="kdgR"
                     /locus_tag="OO566_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6606041.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12417..12656)
                     /locus_tag="OO566_002822"
     CDS             complement(12417..12656)
                     /locus_tag="OO566_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606042.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12815..12958
                     /gene="mgrB"
                     /locus_tag="OO566_002823"
     CDS             12815..12958
                     /gene="mgrB"
                     /locus_tag="OO566_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6606043.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13033..13320
                     /locus_tag="OO566_002824"
     CDS             13033..13320
                     /locus_tag="OO566_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6606044.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13990..14133
                     /locus_tag="OO566_002825"
     CDS             13990..14133
                     /locus_tag="OO566_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6606045.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14146..14355
                     /gene="cspE"
                     /locus_tag="OO566_002826"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14146..14355
                     /gene="cspE"
                     /locus_tag="OO566_002826"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6606046.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14521..15330
                     /gene="rlmA"
                     /locus_tag="OO566_002827"
     CDS             14521..15330
                     /gene="rlmA"
                     /locus_tag="OO566_002827"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6606047.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15327..15893)
                     /gene="mntP"
                     /locus_tag="OO566_002828"
     CDS             complement(15327..15893)
                     /gene="mntP"
                     /locus_tag="OO566_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6606048.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4318 a         3947 c         4133 g         3940 t
ORIGIN      
        1 tagttgaggc cgccgaatgt gccagtcaaa aagtgcgctt tttttgtgcg ctgtcatgcg
       61 ttactggtaa aaagtttccc gtcccgcacc agttcacgcg ggtagctgtt tttcagtcgc
      121 gaaccaatcc gtttggctaa accaatcggc tgatgctgga aagtaaccaa cacgtcatcc
      181 gccactggcg cggcttgcgg gtaaacatcg cgcccgcgat accactcctc cgcttcctgc
      241 ggtgtcagtt caaaagcgtt tacattgtcg ggggaggcat gggcaataac cgcttcatgc
      301 tgccagcgat aacctttgtt atgcgtttcg gcaagtttaa tccctaagcg agaaaatcgg
      361 actttaccga tcagggcttc aatgcctacc gggaacaacc acagttcttt gtcgcgttgc
      421 cagagtcgca ggtttccatc ccagtttaag ccaacacttg cagccgcctg acgaatttgt
      481 ccggcttcgc gatctttcac cgggctgaac gggaaattgc ccactttgta ttttggggcg
      541 ggtaaggcgg ggatcgcctg agttttacgc agacgagcaa cgaagaagcc ttcgcagtcg
      601 taaatttgtg ggaaaacatg caaaaaacct tcttcggtca gcgccttgtt tgcaccaggg
      661 aagagatcgc caagtggtaa aaactctact tcgtcggggt aagtctcttt cagccaccgg
      721 caaacggctt cgttttcttc ccggtttaag gtacaggtcg agtaaaccag cgtaccgcca
      781 ggacgtaatg catgaaaggc gctgtcaatc agttcccgtt gggtagctgc gatttcctga
      841 ttgctttcgg gtgaccagtt ttttagcgca tcgggatctt tacgcaccac gccttcgccg
      901 gagcagggag cgtccagcaa aatggcatca aacatttctg gcactgacgc accaaacacg
      961 cggccaccaa aatgtgtgag cgcaacatta ctgatgccac agcggctgat attggcatgt
     1021 aacactttta cccgactggc ggaaaactca ttggcaagga ttgccccttc gttattcatc
     1081 cgcgcggcaa tttgcgtcgt ttttgagcct ggcgcagcag cgacatccat cacccgctgt
     1141 ggtgcattac cgtcagcaaa caaggcggcg acgggcaaca ttgaactggc ttcctgaata
     1201 taaaacaggc cacttaaatg ctcggtggta ctacccaatg gcaatgcatc ttcattgtcg
     1261 cgttcaatcc agaaaccttc ttcacaccac ggaattggcg taagcgtcca gccataagga
     1321 gcggttaatt gcaggaaatc agcaacggag attttcagcg tattaacgcg aatgctgcgg
     1381 cgcaacgggc gctgacaggc ggcaagaaaa tcatcaaatg agagcgtcga aggcatagct
     1441 tcgcgcattt gtgtcagaaa cgcgtccggg aaataaacgg cgtgttgggc cacgagcatg
     1501 taccactggc aaaaaaacag gcgcgcagtt tagcataaac gctccggcgc aggcacgccg
     1561 gagcagtggg cattatttgg gaagcgcagt tccccattca cgccactctt tcggttcact
     1621 ttcctgcaac aggaagtgtt tgccttcctg ggctttcggt gcaagtggcg tacccggagg
     1681 cgtggcgaag gcgatgccgc cacggataaa ttggttaaag gtgccggttt tcactacgcc
     1741 gcccgtcaga ccaaagtcca gactgtaacc cgatgccaac cagaagacgg aattgttacg
     1801 caccaggtgt tgatagcgtt tactgatgcg catcgcaatc atcacgcgat ctgacaatgt
     1861 ccccagcgtc attccggtaa ccgtacctac ttccagacca cggaacagca caggcgtacc
     1921 gatgcctaac gaaccggctt ccggcgcttc aacaataatg cttaaaccat ctaggtaacg
     1981 cgaatcagta atggtggcct cttctaattc aaagtcgcga cgaggattgc cacggcctgg
     2041 ttcgacgttg atatacggct ggaggatagt atctagatgc tcaacgccag ctgccgagat
     2101 ttgcggtgtg accactgaga agcgcgtacc accgcgagca aaggtctgga catattccgg
     2161 atagagcacc gcctttgcct gtacttcatt gcgtgcggta atcagatcca gcgtctggat
     2221 ttgcccgata tcaataccga gatagcgaat tggcatcccg accgccagtt ttccggcatc
     2281 gaaagcgtga agcgtaatct gcctgccaac cgcacgggcc gctgtttcgg aagcatacag
     2341 aattcgcttg tcgcctttac gctgactggc gctggcacca ctgaggttat cgaagctaat
     2401 ggcccccttt aatgctctgg agagcgggga tgcctgtacg gtcaggccac taccattcag
     2461 ctgaactttc gccccgcctt ctgcccagaa cacgctgttg ctggtcaaaa ggttgcgata
     2521 ttccggttta atgtgcagat cgatatcgaa cgcgttagct cgcggacgca cggtaataac
     2581 ttcaccaact tcaaatttac ggtacagcac taccgatcct gcctgcacat ccggcagcgt
     2641 ctctgcactc aaactcacgg ttgtggtggg taaatcgcta aggctgttct ccagcgcttt
     2701 ttccagattg gcatacagtg gatagctggc tttcatctcg cctttatcgc ccggcagaat
     2761 acgtatcccg ccgttaatcc attctgaggc gctggcaccg agaaactcaa cgccatccag
     2821 ccccaccttc acgtcgacac ggctgttgac gacaaatttg ctatcgcctt ttaccagttc
     2881 tcgatgctga ggctcgatgg cgacggtaaa ggtgacgcct ttgctggtga gtttacgatc
     2941 gataacctgg cctacctgca cgccgtgaag aatgagcggc tgacccgcat caataccgta
     3001 actttccggt gctgtcaggg tcagtgtcag aacgtccggc tcttgcagca gggctttttc
     3061 gcccggcaca acaacgaact ctttgcgtgg ctcgccatcg ccgggcacca actcaaaggt
     3121 ttttccggtc agcagggcgc tgagattagc atcgctaagg gataatttcg ggttgcgtaa
     3181 ttctatgcgg gtattttcac gaagcagggt aacgacgctg ggatcaacgg tcatttcccc
     3241 agtgacttta ccaccaggat ttaaatccag tttagtcagc tgtccgactt ccagcccctg
     3301 atacattaac ggcgtcgagt cggcggttaa tcctgctcca ctcggcagtt ccagtttcac
     3361 cttcgcgcca ctgatactga cgttggcatc aacgccggaa acgttccaga aacggctacc
     3421 ttttttcacc agatcggtaa aacgccgctc gatcaggaca tcaatcacca cgccttgctt
     3481 gttgggattg atggcatagt cgtagacttt tcccaccggg atcttgcgga aatagaccaa
     3541 tgaaccgctg ttcagcgaac cgagatcggg ggcttgcagg tggatcatca gatcgccatt
     3601 gtccagccga tatttcggtt gggtatcgag cgcgacaaag tgatcctgct ctttaccttt
     3661 acccggcatc atgccgatat agttcccacc gacgagggcg tccagcccgg agacacctgc
     3721 caacgatgct tttggcgtca tcagccagaa ctgagtctct tcgcgcagcg catctttcat
     3781 atcggacttg atgctgacct tgacttcaat cttacgaaga tcgtcgctga ggctgatatc
     3841 ctgcactgtt ccgacttcaa cgccctgata acgaacaggc gtacggcccg gaacaatacc
     3901 atccgccgac ataaagtcga tggtgatggt attaccccgg tcctgataac tgtcccaaat
     3961 cagccaaccg gcaatcatta gcgcgatgaa aggcagcagc cagaaaggtg agatacggcg
     4021 tttattttta atctgcgctt cagtcgtcga agcgggcgtt tcctgactca tgtgcatccc
     4081 aaagtaagcg gctgtccagc cattccacag caagaatagt caaaattacg gcagcgccaa
     4141 aataaaacgc agccggtccc atagtaaaag cgaggatctg atcgcgatta atcagcgaca
     4201 tggttaaaga tatgacaaac aggtcgagca tcgaccagcg tccaatccag gtcaccatcc
     4261 gcagtaacag aatgcgtgtg cgtaaacctt gctggcattt aaaatgaatg ctcaacagta
     4321 aagtgaacat gacgatcact ttagtaaacg gtaccagaat actggcgata aacacgattc
     4381 ccgcaaccgc aatgttgcta cttgccagcg acataattcc ggaaagaatc gtatcttcct
     4441 gccggccacc attcagataa atgatagaaa taggcaatag gttggctggt aacaacaaaa
     4501 cgattgacgc taacagcgcc gcccagcatt tttgcaaact atgacgacga cgcaggcgta
     4561 gcgggatatg gcaacgcggg cagagaccac gctgatcagg atagccggta aaatggcacc
     4621 caagacagac acgaagtttc tcgtccctac gcgtagcggg gcgctgcgga taatatcgct
     4681 cccacagctc ctcaacatta agatgtgaca acgtcaccgt cgttaaaatc accaacgcca
     4741 caaaagagaa caagccgaca cccgcctgga tatgggcata atcctgtacc ttatatagaa
     4801 gcaacgccaa tgccgaccag gtagatatcc agcattaccc actctttaag tcgctcaagc
     4861 atcagcagta ccggacgtaa attcattccc agtcggttac caaaccataa atatgctatg
     4921 gaggacacca gaatgagggg ggcaccaata acgcagaaaa agaccattga tcccgttatc
     4981 gcatcgccct gtttggtcat ttgccagatg ccttgcatga cgttggcgtc aatacgaatg
     5041 cctaacagcc agatatgcaa cagcggttcg ccccaggcaa acggcataag caacagcatg
     5101 gtgaaggcca ttgccgccag gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc
     5161 tgacagcgcg gacaataggc actttgatga gaatttatct cgggcaggct aaacagcatg
     5221 tcacattgcg ggcaacgttg gtaatcacca cgcggcagtt cctcgccgat tgccctcact
     5281 gttatctttt ttgtcggcgt aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa
     5341 taagaattaa cattaatctt aacccatgat gggaatgatc ttgagcatta acgcatttaa
     5401 tgcttatttt aataggctgg cagttccagg taagacaacc aagtgattaa ataatgaaca
     5461 aaacagaatt ttacgcggat ttaaatcgcg actttaacgc gctgatggcg ggagaaacca
     5521 gttttctggc aacgcttgcg aacaccagtg cgttgttata cgagcgtctc actgacgtta
     5581 actgggcagg tttttatttg cttgaggacg atacactggt actcggacca tttcagggca
     5641 aaattgcctg tgtccggata cccgtcgggc gcggggtgtg cggcacagcg gttgcccgca
     5701 atcaagtgca gcgtatcgag gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg
     5761 cgagtaattc tgaaattgtt ctgccgctgg tggtgaaaaa tcagattatt ggtgttctcg
     5821 acatcgatag taccgtcttc gctcgcttta cagacgagga cgagcaaggc ttacgtcagc
     5881 ttgtggcaca gcttgaaaaa gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg
     5941 tcgcaggata atcaacggat aacgtagcat ttactggtag cgtcattata atgacgcctg
     6001 ttcatgcctg cgcttgttgg ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac
     6061 ctaagttgaa tagcagtaaa gaagtaatcg cgtttctcgc cgaacgtttt ccccactgtt
     6121 tcagtgcgga aggtgaagcg cgtccgctga aaattggtat ttttcaggat ttggtcgatc
     6181 gtgttgccgg ggaaatgaac ctgagcaaaa cgcaattacg atccgcttta cgtctctaca
     6241 cttcgagctg gcgttatctt tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg
     6301 gcaacccatg cggtgagctg gacgagcaac atgtagagca tgctcgcaag cagcttgaag
     6361 aagcgaaagc gcgtgttcag gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg
     6421 ccgcagctgc tggtgagaaa gaagacgcac cgcgccgcga acgcaagcca cgtccgacca
     6481 cgccacgccg caaagaaggc gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag
     6541 cgccaaaaac agtaaaagca cctcgcgaag aacagcacac cccggtttct gacatttcag
     6601 ctctgactgt cggacaagcc ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg
     6661 tattagaaat caccaaagac ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg
     6721 tgcgcgcaga acacctggtg ttctgaaacg gaggccgggc caggcatgaa catgtttttt
     6781 aggcttaccg cgttagctgg cctgcttgca atagcaggcc agaccttcgc tgtagaagat
     6841 atcacgcgtg ctgatcaaat tccggtatta aaggaagaga cgcagcatgc gacggtaagt
     6901 gagcgcgtaa cgtcgcgctt cacccgttct cattatcgcc agttcgacct cgatcaggca
     6961 ttttcggcca aaatctttga ccgctacctg aatctgctcg attacagcca caatgtgctg
     7021 ctggcaagcg atgttgaaca gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt
     7081 tcaggcaaac tcgacgtttt ctacgatctc tacaatctgg cgcaaaagcg ccgttttgaa
     7141 cgttaccagt acgctttgtc ggtactggaa aagccgatgg atttcaccgg caacgacact
     7201 tataaccttg accgcagcaa agcgccctgg ccgaaaaacg aggctgagtt gaacgcgcag
     7261 tgggacagta aagtcaaatt cgacgagtta agcctgaagc tgacaggaaa aacggataaa
     7321 gaaattcgtg aaaccctgac acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc
     7381 aacagcgaag atgttttctc gctggcaatg atggcgtttg cgcgtgaaat cgatccgcat
     7441 accaactatc tttccccgcg taataccgaa cagttcaaca ctgaaatgag tttgtcactg
     7501 gaaggtattg gcgcagtgct gcaaatggat gatgactaca ccgttatcaa ttcgatggtg
     7561 gcaggtggtc cggcagcgaa gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt
     7621 ggtcaaacag gcaagccgat ggttgacgtg attggctggc gtcttgatga tgtggttgcc
     7681 ttaattaaag ggccgaaggg cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg
     7741 accaagaccc gtactgtaac gttgacccgt gaacgtattc gtctcgaaga ccgcgcggtt
     7801 aaaatgtcgg tgaagaccgt cggtaaagag aaagtcggcg tgctggatat tccgggcttc
     7861 tatgtgggtt tgacagacga tgtcaaagtt caactgcaga aactggaaaa acagaatgtc
     7921 agcagtgtga tcatcgacct gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg
     7981 ctctcaggtc tgtttattcc tgcgggtccc attgttcagg tccgcgataa caacggcaag
     8041 gttcgtgaag acagcgatac cgacgggcag gtgttctata aaggcccact ggtggtactg
     8101 gttgaccgtt tcagtgcttc ggcttcagaa atctttgccg cggcaatgca ggattacggt
     8161 cgtgcgctgg ttgtgggtga accgacgttt ggtaaaggca ccgttcagca ataccgttca
     8221 ttgaaccgta tttacgatca gatgttacgt cctgaatggc cagcgctggg ttctgtgcag
     8281 tacacgatcc agaaattcta tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaaca
     8341 ccagacatca tcatgccgac gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat
     8401 aacgcgctgc cgtgggatag catcgatgcc gcgacttatg tgaaatcagg agatttaact
     8461 gcctttgaac cggagctgct gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc
     8521 cagaacatca tgaaggatat cgcgcgcttc aacgctatga aggacaagcg caatatcgtt
     8581 tctctgaact acgctgtgcg tgagaaagag aataatgaag atgatgcgac gcgtctggcg
     8641 cgtttgaacg aacgctttaa acgcgaaggt aaaccggagt tgaagaagct ggatgatcta
     8701 ccgaaagatt accaggagcc ggatccttat ctggatgaga cggtgaatat cgcactcgat
     8761 ctggcgaagc ttgaaaaagc cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa
     8821 tcaggcacaa gaaattgtgc ctgatttttt aacagcggca agatgccgta aatcagatgc
     8881 tacaaaatgt aaagttgtgt ctttctggtg acttacgcac tatccagact tgaaaatagt
     8941 cgcgtaaccc atacgatgtg ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga
     9001 aaattatgat gcgaatcgcg ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg
     9061 ggctggtact gagcctgaca gggatacagt cgagcagcgt tcaggggctg atgatcatgg
     9121 ccttgctgtt cggttttggt ggttccttcg tttcgcttct gatgtccaaa tggatggcat
     9181 tacgatctgt tggcggggaa gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg
     9241 tcaatactgt agcaacccag gctcgtcagg cggggatcgc tatgccgcaa gtggctatct
     9301 accatgcgcc ggacatcaac gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg
     9361 ctgtcagcac cggtttgctg cagaacatga gcccggatga agccgaggcg gtaattgctc
     9421 acgaaatcag ccacatcgcc aatggtgata tggtcaccat gacgctgatt cagggcgtgg
     9481 tgaacacctt cgttatcttt atttcccgta ttctggcgca gcttgccgcg ggttttatgg
     9541 gcggaaatcg tgatgaaggt gaagagagca acggcaaccc gctgatctac tttgcggttg
     9601 caacggttct ggaactggtg tttggtattc tggcgagcat tatcaccatg tggttctcgc
     9661 gtcatcgtga attccatgct gatgccggtt cggcaaaact ggttggtcgc gagaaaatga
     9721 ttgccgcgct gcagcgcctg aaaaccagct atgaaccgca agaagcaacc agcatgatgg
     9781 ctttctgcat taacggtaag tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc
     9841 tggataaacg tattgaagct ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata
     9901 ctaaaaagcg cgtcctgatc gacgcgcttt ttttatgccc tggctcgtgg ctgagtgata
     9961 cgtaaaccac tgacacaggc agcaatcact gccagaatag ccgcagccat cagcgagacg
    10021 tgcgtaccat tatcaccaaa ctgatttagc atcagcgcca ccagcgccgc gccactactc
    10081 tgacccagta gacgagccgt tcctaacatg ccactggccc caccgctacg ttcgcgcgga
    10141 gcggaggtaa taatggtgtg gttattgggt gactggaata agccaaatcc agcgccacat
    10201 aagatcatcg gccagataat attgatatcc gcaggtgacg cgggcagcag aaccagggaa
    10261 aaaagccccg cagccatgat gaacaaccct aaagcaccca gcaatcctgc atgtacgcgt
    10321 tcaatcaaat agcctgccag cggagccatc accatcgttg ctaacggcca cggtgtcaga
    10381 agtaaacctg tttcgacttc actacgcccg agcacggttt gcaggtaaaa gggcagggaa
    10441 accattgcca gcatttgtgc gcagaaagag caaacagatg tgcaaataga aagtgaaaac
    10501 agcgggatac gcagtaaatc caccggtagc agcggtacgg gaagagaaag ctggcggcga
    10561 ataaagaaaa taccaacaac aaccattacc accagttccg cagcaattaa cgtcagcgat
    10621 tgcccctgag cgaaaccact caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc
    10681 acggcgctgg gcaggtcgaa acggggttta ctggcgcgag aaccattggg tggcagaaaa
    10741 cgcatcgcca gaagcagggc gataatacct aacggtacgt tgattaaaaa taaccatttc
    10801 caggatgcga tggagaggat tgctgcagca attgtcggcc cggcagcaga agagacggca
    10861 acaataaacg agtttatgcc catccctctc cccagaaaac gttgtggata gatcaggcgg
    10921 ataagtgcgg tattaacgct catcaacgct gcaccgccga aaccttgtat gacacgcgca
    10981 agggtgagca tttgcagcga atcagaaagg gcgcagaaca gtgaagacaa cagaaaaacg
    11041 accagaccgc atttataaat acgtcgatag ccaaacatat cgcccagaaa cgaaaacgag
    11101 agcagggaga tgacaatggc gatttgatag gcgttcacta cccagatgga actggctggt
    11161 gtggcatgaa ggtccgtggc gattgttggc agggcgacgt tggcgattgc gccgtcaagg
    11221 acagccatcg aaataccaat cacaatggtt aatatcgcac cgtatcgctg gggcaatggc
    11281 aggccgtcgg cctgaacttt tggcatagga attttatatc tttggtgaat aatcaacagg
    11341 ggtatgattc tagcactgat attcacggtg gatgtcgcag aattgtaact aaccggcaaa
    11401 agatggattg cgaccaccct ggtgcgaaat tataataaaa aacagttctg atttttataa
    11461 aacactcgca atgaggtgat aaatggctaa cgcagatctg gataaacagc ctgattctgt
    11521 atcttccgtg ctaaaagttt ttggcatttt gcaggcgctg ggtgaagagc gcgaaatagg
    11581 gataaccgag ctgtcgcagc gcgtcatgat gtcaaaaagc accgtttatc gctttttaca
    11641 gaccatgaaa accttaggtt atgtggcgca ggaaggggag tcggagaaat attccctgac
    11701 cctgaaattg tttgaactgg gcgctcgcgc gttacaaaac gtcgatttaa ttcgtagcgc
    11761 agatatccag atgcgtgaga tctcccgcct gaccaaagaa actatccacc tcggcgcact
    11821 ggacgaagac agtattgttt acattcacaa aattgactct atgtacaatt tgcgcatgta
    11881 ttcacggatt gggcgtcgta atccgctgta cagcaccgcg attggtaagg tactgctggc
    11941 atggcgcgat cgcgatgaag tgaagcaaat tcttgagggc gtggagtata aacgcagtac
    12001 cgagcggacc atcaccagta cagaagcgtt attacccgtt ctggaccagg tgcgcgagca
    12061 ggggtatggc gaagataacg aagagcagga agaagggctg cgatgcattg cggtaccggt
    12121 atttgatcgc tttggcgtgg tcattgccgg tttgagcatc tccttcccga cgttgcgttt
    12181 ctctgaagag cgtttacagg aatatgtcgc aatgttgcat accgcagcgc gcaaaatttc
    12241 tgcccaaatg ggttatcacg actatccgtt ctgatgagag taagaacctg tcggaatatc
    12301 aaacagacag gttctttatt tagcatgaga aaaataaagt tgaaggtgcg ttatattaaa
    12361 cgcgcttgct ataagaatat tttactcatg agtgagaatc tggttattta ttacccttaa
    12421 ccattatcga ccacgatatt gcttttacgt aacagtgggc aatctgttat ccccaagaaa
    12481 ccacttttag tgtgcaagta ttgtaccgtg ctggtgcctc tggccgtcag ataggtacat
    12541 tgcaaaccta atcctgcggc attctctttg cttccaatca aaacgccata tccgctgagt
    12601 aataatccta tccataccag tgctatcagc ataactgtgc gaatgatgaa tcgcattaca
    12661 atctcttctc tttttatgtt cgcttaatcg tagcggcaat atgcgctgaa gcaagctact
    12721 cattccgaaa aagcacgaat atcgacatag ttaggcgctg tttaactaac gcatgctagt
    12781 ttaatgacat aaggtaggtg aagcggagat tggagtgaaa aagtttcgat gggtcgttct
    12841 ggttgtcgtg gtgttggctt gcttgctgct ttgggcgcag gtattcaaca tgatgtgcga
    12901 tcaggatgta caatttttca gcggaatttg tgccattaac cagtttatcc cgtggtgata
    12961 tcattttttc agcactagat tttcttcctc tccagcagtg gtagaatagc gcctctactc
    13021 agaggtggtg aaatgaacga agttgtaaat tcaggcgtga tgaacattgc gtctttggtt
    13081 gtatcggtgg tggttcttct tatcgggctc atcttgtggt tttttatcaa tcgtgccagt
    13141 tcacggacta acgaacagat tgaactgctt gaggcgttgc tggatcagca aaaacgtcaa
    13201 aatgcactgt tacgtcgttt gtgcgaagca aacgaaccag agaaagcaga taaaaagacc
    13261 attgagagtc aaaaatcggt tgatgacgaa gatattattc gcctggtcgc cgaacgataa
    13321 tccttcctca atcttgagta accccgtcgt tgaagggtta ctcaattcat cctgattata
    13381 cgcactgtca tggtgcaact gcttcaaatt aaaactctgg tctgacttca aataattatc
    13441 atattattta tttgcgtaac gggatatcag aaaaagatat tttcctcatt aatttattag
    13501 gatgtttaca tcagatttgt gattaagcgt ggtattattt attagcgaaa cgtttctctc
    13561 ttgatttttt tgctcactca tcaatttttc ttattttaaa tttacaatcc ctttggggat
    13621 tgacttctct ttagggtaat tgatagccgt taactgactg ttttatgaga aaaagtgata
    13681 taacttttta ttcattgcat agcagaaaat gtgatattgc acgcactatg taataacttc
    13741 tccctctggc ctggaacaac tgaacttatt gaactatgtt agaaactacg ccagtttaag
    13801 tatctgcctg aactggcaag gttaagcaca atgatatatc ggcgcgtatt ccgttgcata
    13861 agtgtgcaaa aaaagtggaa gacgtatcga gatttgtgcg tctgatcgag acatgtttaa
    13921 aaatggcttg ccataattaa cgttgtatgt gataaaagat ttcgggttaa acgaggtaca
    13981 gttctgttta tgtgtggcat tttcagtaaa gaagtcctag gtaaacacgt tgacgttgaa
    14041 taccgcttct ctgccgagcc ttatattggt gcctcatgca gtaatgtgtc agttttatct
    14101 atgttatgcc tgcgggcgaa gaaaacaatc taaggaattt ttcaaatggc aaagattaaa
    14161 ggtcaggtta agtggttcaa cgagtctaaa ggttttggct tcattactcc ggctgatggc
    14221 agcaaagatg tgttcgtaca cttctccgct atccagggta atggcttcaa aactctggct
    14281 gaaggtcaga acgttgagtt cgaaattcag gacggccaga aaggtccggc agctgttaac
    14341 gtaacagcta tctgatcgaa tccactgatc tgaagtgtga atacgcttca atctcgctat
    14401 aaagcctcgt cgaatgcgag gctttttact atgctttatc ttcgctcctg gcgttcggat
    14461 atttgccagc cacgtgattc gcgttacact tgcggccttt agtatcctgc cggagttgtc
    14521 atgtcgtttt cctgtccact ttgccatcag cctctttcgc gtgaaaaaaa cagctatatc
    14581 tgtccccaac gacatcagtt tgatatggcg aaagaagggt atgtcaatct gctgcccgtt
    14641 cagcataaac ggtctcgtga tccgggcgac agcgcggaaa tgatgcaagc acgccgcgca
    14701 ttcttagatg ccggacatta tcagccgctg cgtgatgcaa ttgtcgccca actgcgggaa
    14761 cggcttgatg aaaaggccac ggcggtgctg gatattggct gtggtgaagg gtattacaca
    14821 cacgcatttg ccgatgcgtt gcccgaaatc accacgtttg gcctggatgt ctcgaaggta
    14881 gcgataaaag cggcggcgaa acgctatccg caggtcactc tttgtgtcgc ttccagccac
    14941 cgtttgccgt tttccgatac cagtatggac gccataatac gtatttacgc gccgtgtaaa
    15001 gcagaagaat tagcacgagt agtgaagccc ggcggctggg tcattactgc cacgccggga
    15061 ccgcgacatt tgatggagct gaaggggctg atttacaatg aagtacatct tcatgcacct
    15121 catgcagaac aactggaagg ttttacatta cagcagagtg cggagttgtg ttatccgatg
    15181 cgtcttcgcg gtgatgaagc cgtcgcatta ttgcagatga cgccgtttgc ctggcgtgcg
    15241 aagccagaag tctggcaaac actggcagca aaagaagtgt tcgactgcca gacggacttt
    15301 aatattcacc tctggcagcg ttcttattaa ccgtggaagt gcgtccagag gatctggacg
    15361 ccgatgccga tcagcaccag cccgccgaga atttccgctt ttttcccaat aattgagccg
    15421 ataaagcgac caaccatcat ccctaatgtc gacataatca aggttgcaca accaatggcc
    15481 aatgcggtcg cgataatgtt gacctgaagg aaagcaagac caacacccac agccatggca
    15541 tccaggctgg tggcaatcgc ggtggttacc agtagccaga aaccgtgccg acggcgcggc
    15601 tcttcatctt catcatctga gccacgaaaa ccctcaataa tcattcgccc gccgaggaat
    15661 atcagcagca caaacgcaat ccagtgattc cattcaagga caaaccggct ggctaacatg
    15721 cccattcccc agccgatcag cggcgtcagg gtttcgacgg caccaaaaat aaggccggtt
    15781 cgcaatgctt cagaaaattt cggtttatgg aggggggcac ctttaccgat tgatgcagca
    15841 aatgcatcca tcgacatacc aaacgcaaga agaacagtag cagtgatatt catgacaacg
    15901 tcctgaccgg ggtatccata aaacacatca tcgcccccag caaacatgca tacatgaaca
    15961 acaagccgcg atgggttttc gcgttcatgc aggcatagcg attgatgtgc atatggtctc
    16021 gcctgactga aagtcttcag tccgtacgtg ccacgttttg caacgagtat gttgacacgt
    16081 acatttccgg aatctggaaa tcggctactc cccaaatgag ggcgcaacct taacatattt
    16141 tgagaatata aaacaactgt tggaatatat tatttcattg ctttattgat aacgattttc
    16201 atttatgttt atatatcaat gaaacgttaa aataaataac aaataaggga agagataata
    16261 tagcctaagc tatgtctgat tattaaaaat acactaaaca tagctttggc taaattcata
    16321 agttattgag ttgatacg
//
LOCUS       DAJTXY010000086        16311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTXY010000086 DAJTXY010000000
VERSION     DAJTXY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.086"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="OO566_002829"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="OO566_002829"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6606049.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYEVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="OO566_002830"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="OO566_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6606050.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="OO566_002831"
     CDS             2337..2552
                     /locus_tag="OO566_002831"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606051.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="OO566_002832"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="OO566_002832"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6606052.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /gene="galP"
                     /locus_tag="OO566_002833"
     CDS             4447..5841
                     /gene="galP"
                     /locus_tag="OO566_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6606053.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWRTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="OO566_002834"
     CDS             5918..6415
                     /locus_tag="OO566_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6606054.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="OO566_002835"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="OO566_002835"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6606055.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="OO566_002836"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="OO566_002836"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6606056.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="OO566_002837"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="OO566_002837"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6606057.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9100..9663
                     /locus_tag="OO566_002838"
     CDS             9100..9663
                     /locus_tag="OO566_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6606058.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="OO566_002839"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="OO566_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6606059.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="OO566_002840"
     CDS             complement(10255..11235)
                     /locus_tag="OO566_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6606060.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="OO566_002841"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="OO566_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6606061.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="OO566_002842"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="OO566_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6606062.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="OO566_002843"
     CDS             12538..12828
                     /locus_tag="OO566_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6606063.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANNHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="OO566_002844"
     CDS             12836..13429
                     /locus_tag="OO566_002844"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6606064.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITHEPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="OO566_002845"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="OO566_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6606065.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="OO566_002846"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="OO566_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..>16311
                     /locus_tag="OO566_002847"
     CDS             15880..>16311
                     /locus_tag="OO566_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR6606066.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVM"
BASE COUNT         4037 a         4208 c         4104 g         3962 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttctg cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttctaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaggc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cctcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga atcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaaaaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggtcgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggtctgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg cctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaattga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 acttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac catattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtgga ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtctcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 agccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcactcgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccga ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gcgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgatcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata ccatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgtcatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacggc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa caatcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cacgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgttcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ccatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aagcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtgac gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattacc
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat g
//
LOCUS       DAJTXY010000087        16151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTXY010000087 DAJTXY010000000
VERSION     DAJTXY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.087"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(188..520)
                     /gene="emrE"
                     /locus_tag="OO566_002848"
     CDS             complement(188..520)
                     /gene="emrE"
                     /locus_tag="OO566_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6606067.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(865..1179)
                     /gene="fliE"
                     /locus_tag="OO566_002849"
     CDS             complement(865..1179)
                     /gene="fliE"
                     /locus_tag="OO566_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6606068.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1393..3051
                     /gene="fliF"
                     /locus_tag="OO566_002850"
                     /pseudo
     CDS             1393..3051
                     /gene="fliF"
                     /locus_tag="OO566_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            3044..4039
                     /gene="fliG"
                     /locus_tag="OO566_002851"
     CDS             3044..4039
                     /gene="fliG"
                     /locus_tag="OO566_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6606069.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4032..4718
                     /gene="fliH"
                     /locus_tag="OO566_002852"
     CDS             4032..4718
                     /gene="fliH"
                     /locus_tag="OO566_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6606070.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            4718..6091
                     /gene="fliI"
                     /locus_tag="OO566_002853"
     CDS             4718..6091
                     /gene="fliI"
                     /locus_tag="OO566_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6606071.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRSQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6110..6553
                     /gene="fliJ"
                     /locus_tag="OO566_002854"
     CDS             6110..6553
                     /gene="fliJ"
                     /locus_tag="OO566_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR6606072.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6550..7677
                     /gene="fliK"
                     /locus_tag="OO566_002855"
     CDS             6550..7677
                     /gene="fliK"
                     /locus_tag="OO566_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6606073.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSASLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGDDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7782..8246
                     /gene="fliL"
                     /locus_tag="OO566_002856"
     CDS             7782..8246
                     /gene="fliL"
                     /locus_tag="OO566_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6606074.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8251..9255
                     /gene="fliM"
                     /locus_tag="OO566_002857"
     CDS             8251..9255
                     /gene="fliM"
                     /locus_tag="OO566_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6606075.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDILPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9252..9665
                     /gene="fliN"
                     /locus_tag="OO566_002858"
     CDS             9252..9665
                     /gene="fliN"
                     /locus_tag="OO566_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6606076.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9668..10033
                     /gene="fliO"
                     /locus_tag="OO566_002859"
     CDS             9668..10033
                     /gene="fliO"
                     /locus_tag="OO566_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6606077.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10033..10770
                     /gene="fliP"
                     /locus_tag="OO566_002860"
     CDS             10033..10770
                     /gene="fliP"
                     /locus_tag="OO566_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6606078.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10780..11049
                     /gene="fliQ"
                     /locus_tag="OO566_002861"
     CDS             10780..11049
                     /gene="fliQ"
                     /locus_tag="OO566_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6606079.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11058..11762
                     /locus_tag="OO566_002862"
     CDS             11058..11762
                     /locus_tag="OO566_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCR6606080.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIFLNGLMLA
                     LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFS
                     EIFNLLADIISELPLI"
     gene            12047..12670
                     /gene="rcsA"
                     /locus_tag="OO566_002863"
     CDS             12047..12670
                     /gene="rcsA"
                     /locus_tag="OO566_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6606081.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12714..12902)
                     /gene="dsrB"
                     /locus_tag="OO566_002864"
     CDS             complement(12714..12902)
                     /gene="dsrB"
                     /locus_tag="OO566_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6606082.1"
                     /translation="MKVNDRVTVKTDSGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13065..13292
                     /gene="yodD"
                     /locus_tag="OO566_002865"
     CDS             13065..13292
                     /gene="yodD"
                     /locus_tag="OO566_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6606083.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13590..14405
                     /locus_tag="OO566_002866"
                     /pseudo
     CDS             13590..14405
                     /locus_tag="OO566_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(14402..16096)
                     /gene="yedQ"
                     /locus_tag="OO566_002867"
     CDS             complement(14402..16096)
                     /gene="yedQ"
                     /locus_tag="OO566_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR6606084.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDHA"
BASE COUNT         4022 a         4156 c         4237 g         3736 t
ORIGIN      
        1 aaacgataag ttttttcata aaaacctcat tgttgtgacc tatcgttatt ttatttgatg
       61 tgccttgatc tcattatgca taaaggcaca atgatcgcgc aaatgcaatc ataattataa
      121 aggtgccccg aacattagcc ataatatgca caaatcagat tcctgttatc tcaaggagaa
      181 ttatttttta atgcggtgca cttcgtgaca acaagttaat gactaacaca ccggcacaaa
      241 tcaacatcat gcctatgata gctggcaggt ccagtcgttg accaaaaaat ccccaggaaa
      301 gcaaactaat cagaacgata ccgactcctg accagatagc ataagcaatc cctgtaggta
      361 tataagccag cgtctgagct aataaccaga atgatgcaca atagcaaata attgtgccaa
      421 cagagggcca taaccgtgta aacccctctg aaaacttcat taaggttgta ccaatgacct
      481 ctgcaagtat tgcgccacca agataaatat aagggttcat agcatattct ttcctgttca
      541 tactggagag aattgtacta cagtttgaac acaactcacc gttttcatcc tagtgtatgt
      601 gtatccaagg ggctctggcg tggcaagaga taaaggcagg aacaataatg atcagaagga
      661 accccaggct aacgaatgag ttcactcttc ctgggagcaa agcgaagaca aaacatgtta
      721 tcgcgtttac ggatcgtgga tggcagaaaa caaccaggat caggtatcca ttttaaacca
      781 gaaattgagt gagtttgccc catccatgcc ccacgctgtg ggatcggata taattaaaca
      841 agcatagtca acaggttaca caacctacac ctgcatagac atcacttcct gatacgccgc
      901 caccagctta ttacgcacct gaatccccat ttgcatagaa actgaggctt tttgcatatc
      961 ggtcatcaca tcgtttagcg ccacgccggg ttcaccgagg gtgaattttt ctgcctgcgt
     1021 gcgggcgact gtttgtgtat cgcttatgcg atcgatcgcg gcgtgcagct gcccggcaaa
     1081 actaatggtc ggttgcggca gtgattcctg cgcacgcgca ctcatcgccg tagcctgtaa
     1141 ctggctgata accccttcaa tcccctgtat cgctgacatt ttcgtctccc ggataatttc
     1201 tggtaacaaa gcctaccagt aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac
     1261 gggtttattg gcggatagaa aaaacgaaag cacaaataat gggagcgtca atttttcgag
     1321 tttgctgacc cgagagtgag tcttgttcca ctttgccaat aacgccgtcc ataatcagcc
     1381 acgaggtgcg cgatgaatgc gactgcagcc cagacaaaat ctcttgagtg gcttaatcgc
     1441 ctgcgtgcga atccgaaaat tccattgatt gttaccggtt ccgcggcagt ggcggtcatg
     1501 gtcgcactga tcctgtgggc gaaagccccc gactaccgca cattattcag caatctttcc
     1561 gatcaggatg gtggcgcaat tgtcagccaa ctgacgcaaa tgaatattcc ttaccgcttc
     1621 agcgaagcca gcggcgctat tgaggttccg gcagataaag ttcacgaact gcgcctgcgc
     1681 ctggcgcaac aaggtttgcc caaaggcggc gcggtcggtt tcgaactgct cgatcaggaa
     1741 aagtttggta tcagccagtt cagcgaacag gtgaattatc agcgggcgct ggaaggcgag
     1801 ctttctcgta ccatcgaaac tatcggcccg gtaaaagggg cgcgcgtaca tctggcaatg
     1861 ccgaaaccgt ctttattcgt ccgtgaacaa aaatcccctt ctgcatcggt gacggtaaat
     1921 ctgttaccca gccgcgcact cgatgaaggg caaattagcg ctattgtaca tctggtttcc
     1981 agcgcggttg ctggtctgcc gcctggaaac gtcacgctgg tggatcaggg cgggcatctg
     2041 ttaacccagt ccaataccag cgggcgcgat cttaatgacg ctcagttgaa atatgccagc
     2101 gatgtcgaag gccgtattca gcggcgtatt gaagcgatcc tgtcgcctat tgttggtaac
     2161 ggtaatattc acgcctaggt cacggcgcaa ctggacttcg ccagtaaaga acaaacggaa
     2221 gaacagtatc gccctaacgg tgatgaatct catgcggcgc ttcgttcacg ccagcttaat
     2281 gagagcgagc aaagcggttc cggttatccg ggcggcgtac cgggggcgtt gtcgaatcaa
     2341 ccggcacctg cgaataacgc gccaatcagc acgcctccga caaatcaaaa taaccgccag
     2401 cagcaggcga gcaccaccag caatagtggg ccgcgtagca cacagcggaa tgaaaccagt
     2461 aactacgaag tcgatcgcac cattcgtcat accaaaatga acgtgggcga tgtgcaacgt
     2521 ctgtcagtcg cggtagtggt gaattacaaa accttgccag acggtaaacc attgcctctc
     2581 agcaacgaac agatgaagca aattgaagct ctgacccgcg aggcgatggg cttttctgaa
     2641 aaacgcggtg attcgctcaa tgttgttaac tcgccgttca atagcagtga cgaaagcggc
     2701 ggagcactgc cgttttggca acagcaagtg tttatcgatc agttgctggc tgccggtcgc
     2761 tggttgctgg tactgctggt ggcgtggttg ctgtggcgga aagcggtacg cccgcagcta
     2821 acacgtcgcg ccgaggcggt gaagactgtg cagcaacagg cgcaggcccg cgaggaagtg
     2881 gaagatgcgg tggaagtccg tctgagcaaa gacgaacaac tccaacaacg gcgcgctaac
     2941 caacgactgg gggcagaagt catgagccag cgtatccgtg aaatgtctga taacgatccg
     3001 cgcgtggtgg cgctggtcat tcgccagtgg ataaataacg atcatgagta acctgacagg
     3061 caccgataaa agcgtcatcc tgctgatgac cattggcgaa gaccgggcgg cagaggtgtt
     3121 caagcacctc tcccagcgcg aagtgcaaac cctgagcgct gcaatggcga acgtcacgca
     3181 gatctccaac aagcagctaa ccgatgtgct ggcggagttt gagcaagaag ctgaacagtt
     3241 tgctgcactg aatatcaacg ccaacgatta tctgcgttcg gtattggtca aagctctggg
     3301 tgaagaacgt gccgccagcc tgctggaaga tattctcgaa acccgcgata ccgccagcgg
     3361 tattgaaacg ctcaacttta tggagccgca gagcgccgcc gatctgattc gcgatgagca
     3421 tccgcaaatt atcgccacca ttctggtgca tctgaagcgc gcccaggccg ccgatattct
     3481 ggcgttgttc gatgaacgtc tgcgccacga cgtgatgttg cgtatcgcca cctttggcgg
     3541 cgtgcagcca gccgcgctgg ccgaactgac cgaagtactg aatggcttgc tcgacggtca
     3601 gaatctcaag cgcagcaaaa tgggcggcgt gagaacggca gccgaaatta tcaacctgat
     3661 gaaaacgcag caggaagaag ccgttattac cgccgtgcgt gaattcgacg gcgagctggc
     3721 gcagaaaatc atcgacgaga tgttcctgtt cgagaatctg gtggatgtcg atgatcgcag
     3781 cattcagcgt ctgttgcagg aagtggattc cgaatcgctg ctgatcgcgc tgaaaggggc
     3841 cgagcagcca ctgcgcgaga aattcctgcg caatatgtcg cagcgtgccg ccgatattct
     3901 gcgcgacgat ctcgccaacc gtggtccggt gcgtctgtcg caggtcgaaa acgaacagaa
     3961 agcgattctg ctgattgtgc gccgtcttgc cgaaactggc gagatggtga ttggcagcgg
     4021 cgaggatacc tatgtctgat aatctgccgt ggaaaacctg gacgccggac gatctcgcgc
     4081 caccaccggc agagtttgtg ccaatggtcg agtcggaaga aaccattatt gaagaggccg
     4141 aacccagcct tgagcagcaa ctggcgcaac tgcaaatgca ggcccacgag cagggttatc
     4201 aggcggggat tgccgaaggt cgccagcaag gtcacgaaca gggctatcag gaaggactgg
     4261 cccaggggct ggaacaaggt ctggcagagg cgaaggctca acaagcgcca attcatgccc
     4321 ggatgcagca actggtcagc gaatttcaaa ctacccttga tgcgcttgat agtgtgatag
     4381 cttcgcgcct gatgcagatg gcgctggagg cagcacgtca ggtgatcggt cagacaccaa
     4441 cggtggataa ctcggcactg atcaaacaga tccaacagtt gttgcagcaa gaaccgttat
     4501 tcagcgggaa accacagctg cgcgtacacc cggatgatct gcaacgtgtg gatgatatgc
     4561 tcggcgcaac tttaagtttg catggctggc gcttgcgggg cgatcccacc ctccaccctg
     4621 gcggatgtaa agtctccgcc gatgaaggcg atctcgacgc cagtgtcgcc actcgctggc
     4681 aagaactctg ccgtctggca gcactaggag tggtgtaatg accacgcgcc tgactcgatg
     4741 gctaaccacg ctggataact ttgaagcaaa aatggcgcag ttgcctgcgg tacgtcgcta
     4801 cgggcgatta acccgcgcta ccgggctggt gctggaagcc accggattac aattgccgct
     4861 cggcgcaacc tgtgtcattg agcgccagaa cggcagcgaa acgcacgaag tagaaagcga
     4921 agtcgttggc tttaacggtc aacggctgtt tttaatgccg ctggaggaag tggaaggtgt
     4981 cctgcccggc gcgcgtgtat atgccaaaaa catttcggca gaagggctgc aaagcggcaa
     5041 gcagttgccg ctcggtccgg cgttattagg tcgtgttctg gacggtagcg gtaaaccgct
     5101 cgatggcctg cccttccccg atacgacgga aaccggtgcg ctgattaccc cgccatttaa
     5161 cccgttgcaa cgtacaccga ttgaacatgt gctggacacc ggcgtgcgcc caatcaatgc
     5221 cctgcttacc gtcggacgcg ggcagcgtat ggggctgttt gccgggtcgg gcgttggtaa
     5281 aagtgtgctg ctggggatga tggcacgtta cacccgcgcc gatgtcattg tcgtgggttt
     5341 gattggtgaa cgtgggcgcg aagtaaaaga ttttattgag aatatcctcg gtgccgaagg
     5401 acgtgcacgc tcagtggtga ttgccgctcc ggcggatgtt tctccgctcc tgcgaatgca
     5461 aggtgccgcc tatgccacgc gcattgccga agattttcgc gatcgtagtc agcatgtgtt
     5521 gctgattatg gactccctca cccgctacgc gatggcccag cgtgaaattg cgctggcgat
     5581 tggcgaacca cctgccacta aaggttatcc accgtcggtg tttgccaaat taccggcact
     5641 ggtcgagcgt gccggtaatg gcattagcgg cggcggctcg attaccgcgt tttataccgt
     5701 gctcaccgaa ggcgatgacc agcaggaccc cattgctgac tccgcgcggg caatcctcga
     5761 cggtcacatt gtgctgtctc gccgactggc ggaagccggg cactatccgg ctatcgatat
     5821 tgaagcgtcg atcagtcgcg caatgacggc gttgatcagt gagcaacatt acgcgcgagt
     5881 gcgcaccttc aaacagctgt tgtcgagttt tcagcgtaac cgcgatctgg ttagcgtcgg
     5941 cgcgtatgcc aaaggcagcg atccgatgct cgataaagcc atcgccctgt ggccgcagct
     6001 ggagggctat ttgcaacaag gcatttttga acgcgcggac tgggaagcgt ctctccaggg
     6061 gctggagcgt attttcccga cagtgtcata acccaggaga taacggcaga tggcggaaca
     6121 tggtgcgctg gcgaccctga aagatctggc agaaaaagag gtagaggatg ccgctcgcct
     6181 gctgggtgaa atgcgtcgcg gatgtcagca ggcggaagaa cagctcaaaa tgctgatcga
     6241 ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga tgaccagcaa
     6301 ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta ctcagcatcg
     6361 ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct ggcgagaaaa
     6421 gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg cggcactgct
     6481 tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg ccgccatgag
     6541 gaaacctgaa tgattcgctt agcgcccttg attaccgccg acgttgacac caccacattg
     6601 cctggcggca aagccagcga tgctgcacaa gattttctaa cgttgttgag cgaagcatta
     6661 gcaggcgaga caacaactga caaagcggcc ccccagttgc tggtggcaac agataagccc
     6721 acgacaaaag gcgagccgct ggtcagcgat attcttgccg atgcgcaaca agcggattta
     6781 ctgatccctg tggatgaaac acttcctgtc atcaacgacg aacaatccac atcaacaccg
     6841 ttaaccaccg ctcagacaat gacgttggct gcggtggctg acaaaaatac gacaaaagac
     6901 gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc tgagcgccct ttttgcgatg
     6961 ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt gttaccggca
     7021 gagaaaccaa cgctcttcac aaaactgact tctgcgcaac tcactacagc acagcctgat
     7081 gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc ccagagtaaa
     7141 gcggaagtca tcagcacgcc ttcgccggtg accgctgacg ccagcccgct aatcactcca
     7201 caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcatc gctgggttct
     7261 cacgaatggc aacaatcatt gagccagcat atttcgctgt tcacccgcca ggggcaacaa
     7321 agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc cctcaaagtg
     7381 gatgataacc aggcgcagat ccagatgatt tcaccgcatc agcacgtacg cgccgccctg
     7441 gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca gttagggcaa
     7501 agcaacatta gtggcgaaag ctttagtggt cagcagcagg ccgcttccca gcaacagcaa
     7561 agccaacgca cagcaaacca tgaacctctg gcgggggatg acgacgatac gcttccggtt
     7621 cccgtctctt tacaagggcg tgtaacaggt aacagcggcg ttgatatttt cgcctaacgt
     7681 cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag acgcaggata
     7741 attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat tacgcgataa
     7801 gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc ctcgcggcct
     7861 gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac gacaaagcgc
     7921 agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg gtcaatttgg
     7981 gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg actgaaagat gaagcaaccc
     8041 gctcgcggct gagtgagtat ttgccggaag tccgtagtcg cttgctgtta ctgttttcac
     8101 gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt gccgagatta
     8161 aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc accgacgtgc
     8221 tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc tcaagctgaa
     8281 attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc cagtgttagc
     8341 ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt gcgcgaacgt
     8401 ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat ggggctgttc
     8461 aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca gccataccat
     8521 gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc taatccacct gaaaccgcta
     8581 cgcggcacag ggctggtagt gttctcgccg agtctggtgt ttatcgccgt ggataacctg
     8641 tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac ccatactgaa
     8701 cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag gctatagcga cgcctggaaa
     8761 gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga atttaccaat
     8821 atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga gattggcaac
     8881 ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct acgggaattg
     8941 ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg cgataacctg
     9001 gtgcgccagg tgcagcattc acagctggaa ctggtggcca actttgccga tatctcgcta
     9061 cgcctgtcgc agattttaaa actgaaaccc ggcgacatcc tgccgataga aaaacccgat
     9121 cgcatcatcg cccatgttga cggcgtcccg gttctgacca gtcagtacgg cactctcaac
     9181 ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc tctgaacgag
     9241 gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc gcaatggacg
     9301 atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc gctgccgacg
     9361 cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat atcgacctga
     9421 ttatggatat tccggtcaag ctgaccgtcg agctgggccg tacgcggatg accatcaaag
     9481 agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg ggcgaaccgc
     9541 tggatattct gaacaacggt tatttaatcg cccaaggcga agtggtggtt gttgccgata
     9601 aatatggcgt gcggatcacc gatatcatta ccccgtccga gcggatgcgc cgcctgagcc
     9661 gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttctg ctgagccact
     9721 gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg ctgcctggct
     9781 ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt aacggtctga aaattagcgc
     9841 cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag atgcacggct
     9901 ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc cttcagcacc
     9961 aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaatttgc ttaagcgtgg
    10021 cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg gctgattatg
    10081 cccctcgcct tcgcgcaact gccgggtatc accagccagc cgctacctgg cggtggacaa
    10141 agctggtcgc tcccggtgca gacgctggtg ttcatcacct cgttgacgtt cattccagcg
    10201 attttactga tgatgaccag tttcacccgc atcattattg tttttggttt attgcgtaac
    10261 gcgctgggaa cgccctccgc gccacctaac caggtattgc tggggctggc actgtttttg
    10321 acctttttta ttatgtcgcc agtgatcgac aaaatttatg tcgatgcgta ccagccattc
    10381 agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc gctgcgtgag
    10441 tttatgctgc gtcagacccg cgaggcagat ttagggttgt ttgccagact ggcgaatacc
    10501 ggcccgttgc aggggcctga agctgtaccg atgcgcattt tgctcccggc ctacgtgacc
    10561 agcgagttga aaaccgcatt tcagataggc ttcacgattt tcatcccttt tttgattatc
    10621 gacctggtga ttgccagcgt gttgatggca ttggggatga tgatggttcc ccccgccacc
    10681 attgctctgc cctttaaact gatgctgttt gtactggtgg atggctggca attgctggtc
    10741 ggttcgctgg cgcagagctt ttacagctag agaggcaaaa tgacacctga atcggtcatg
    10801 atgatgggaa ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct gttgctggta
    10861 gcgctggtca cgggccttat catcagtatt ttgcaggcag ccacgcagat taacgaaatg
    10921 acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg ccatcattat tgccggaccg
    10981 tggatgctca atctgttgct ggattacgtc cgcaccttgt tcactaacct gccgtatatc
    11041 atcgggtagc ccgtacaatg atgcaggaga caagcgatca atggctttcc tggttaagcc
    11101 tgtacttctg gccgttactg cgcgtgctgg cgctcatttc taccgcgcca attctgagcg
    11161 aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg ttcgccattg
    11221 ccccatcatt acctgccaac gatgttcctg ttttttcgtt ctttgctctg tggctggccg
    11281 tgcagcagat cctgatcggc attgcgcttg gttttaccat gcaatttgcc tttgccgctg
    11341 tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct gtcatttgcg acatttaacg
    11401 gtcatttatg gttgatttca ctgctggtcg atacctttca caccctgccg attggtagcg
    11461 aaccgttgaa cagcaatgcg tttctggcac ccaccaaagc agggagtttg attttcctta
    11521 acgggctgat gctggcgtta ccgctcatta ctctgttgct gaccctgaat ctggcattag
    11581 gtttactgaa tcgtatggcc ccgcaattat ccatttttgt tattggattt ccattaactc
    11641 tgactgtcgg catctcttta atggcggcat taatgccgtt aattgcgcct ttttgcgaac
    11701 atttattcag tgaaattttt aatttgctgg ctgatattat tagtgaatta ccattaatat
    11761 aattccgtaa cgtttatcat gctaccctaa ggattatccg aaaaataata tctgcgaaca
    11821 tcttccagga tactcctgta gcgaaatatt tgttttaagc tcactcacat atcgcaacat
    11881 ttactttaag acaattccag gcaaattata caacacttta cgggatagta agtccgcctg
    11941 aaaaattgcg agagtggcgc attaggtgac ccatgttgtt ccgtttagtc atgatgaaat
    12001 attcaggtaa ggggaattat cgttacgcat tgagtgaggg tatgccatgt caacgattat
    12061 tatggattta tgtagttaca cccgactagg tttaaccggg tatctgttga gtagaggggt
    12121 taaaaaaaga gaaatcaacg acattgaaac cgttgatgac cttgccatag cttgtgattc
    12181 acagcgtcct tcagtggtgt ttattaatga ggactgtttc atccacgatg cttctaacag
    12241 tcagcgcatc aagcacatca ttaatcaaca tcccaatacg ttatttatcg tttttatggc
    12301 aattgccaat gttcattttg atgaatatct attggtcaga aaaaatttat tgatcagttc
    12361 taaatcgatt aaaccggaat ctctcgacga tatccttggc gatattctga aaaaagagac
    12421 aacgataacc tcgtttttaa atatgccgac gttatcattg agccgaaccg aatcgagtat
    12481 gttgcgaatg tggatggcag gtcagggaac cattcaaatc tccgaccaaa tgaatatcaa
    12541 agccaagacc gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa cgcataataa
    12601 acaggttatc taccatgtcg tccgactgac ggataatgtg actaatggta tttttgtcaa
    12661 catgcgctaa cacattctga ctggtggttt cccaccagtc aggctgaata agattactct
    12721 gctttctcaa caaagatacc gtcctgatgc cctgcttcat taaagaacca gatgccgagc
    12781 gggtagtctt ccagcgcaac caggtacatt gtgccttcac taaactcctc aactgccagt
    12841 accactccag gacgacgcgg accgctatcc gttttgactg ttacccgatc attcaccttc
    12901 atcgttttcc tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc tggcagcgcc
    12961 aagcgcgcgc gagtgctgat tttctcgacg gtctatactt aagagatgcc aggcggactt
    13021 aacgactggc ggcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa accgcaaaag
    13081 tgtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg ctggcggcga
    13141 tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa cgattctgaa cacgttagcg
    13201 tcgatggacg tgaatatcat acatggcgtg aattggcgga tgccttcgaa ctggatattc
    13261 atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc ccgaccctga
    13321 gggtgtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt acaccaggaa
    13381 atctgatgtg ttcatcacct tatctgcaat ttttttcgct gacaagaaaa tattcgcgct
    13441 atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct tggtggctct
    13501 ggtgtcaacg ggatcgctcc cgaaaaaatc atctttgatt atttgtgctg ttttgagaat
    13561 catcctggta aggggagtac tgcgcgacca tgttttcaat tcaacaacca ctactggttt
    13621 ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa ccggcagccc
    13681 cctggctcag ccgtttacac gaagccaata ttcccgtcat tctctgtagc agtaaaactt
    13741 cagcggaaat gctgtacttg caaaaaatgt tggggctaca aggcttaccg ctgattgcag
    13801 agaatggcgc agtgatccag cttgctgaac aatggcagga tatagacggt tttccacgca
    13861 tcatctcagg tattagccat ggcgaaatct gccaggtttt aaatacgcta cgcgagaaag
    13921 aacattttaa attcacgact tttgatgatg tcgacgatgc aaccattgcc gaatggacgg
    13981 gattaagccg tagctaggcg gcgctgacgc agctgcatga ggcgtcggta acgctaatct
    14041 ggcgcgacag tgacgagcgt atggcacaat ttatcgctcg tctgaacgaa ctgggcttgc
    14101 agtttatgca gggtgcgcgc ttctggcacg tactggatgc ctctgccgga aaagatcagg
    14161 ctgccaactg gattatcgcg acctatcaac aattgtcagg cagacgccca accacacttg
    14221 gcctgggcga tgggccaaac gatgcgcctt tactggaggt aatggattac gcggtgattg
    14281 tgaaagggct aaaccgtgaa ggggtacatc tgcatgatga ggatccggcc cgcgtctggc
    14341 gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt ttctccgccc
    14401 gttaagcgtg atcgctcgcg aatacccgat tgcgcccggc ctgttttgcg aggtaaagcc
    14461 gacggtcggc caatgactgg agttgttcaa aatcataatc accggtttcc tcgctgctac
    14521 ttacccccag cgaggcactg atgcgtatcg ttgtactctt ggcgatcagc atctcttttt
    14581 catttaactt caggcgaata cgttctgcga cctccgcagc ctgcgtcaga ttcgcgcctg
    14641 gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacgtcctgc gcacgcaagg
    14701 aactgctaat taatccggca gcatgagaaa gaacacggtc gcccgcctga tgaccaaagc
    14761 ggtcattaat cgctttaaaa tggtcaagat cgacctgaat gacagaaaaa ggatgttggt
    14821 gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca cggttatata
    14881 aacgcgttaa ggtgtcgtgc cacgcctgcc actgcaacga gctttgcaga acatacatgt
    14941 tgctgaccat ccggcgaatc acataccagg aaaggagtaa catggtggta aagagcgccc
    15001 acagcagggt taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct tcgcttagcg
    15061 tatggacgcg cgccagcaca ccgtcgaaat ggtccagacg ttcccagcta acatagcgac
    15121 tgtccatacg aatgccgccc cgcgtgtcat gttccattgc ctgcgccagc aaggccagtt
    15181 cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc aaaaatatca
    15241 gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg tttcttaaaa
    15301 attgctgcat agtacgcacg ggaatactca tccccagcac gccataccag tagttattac
    15361 tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt tccggttgcg
    15421 aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg ataaaccagg
    15481 gttgggtgac atagccgtaa taacgcgttg gtacattgcg cataaacaag gtcggctgcg
    15541 tcgaaacgta aaatccagca cgcgagacat acatcgcctg ttcaaccatc gacgaggagt
    15601 tgtgcgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca ttgtcgaggc
    15661 tttcattttc gcgagacagg aaattcccct cgctgactaa tgcatccgat acgccattga
    15721 ctggcagggt gcgccgtcgg ttgagttcga tttgccaggc gtgctcgtcg cgatgctgtt
    15781 cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgccacg agagcttcgc
    15841 gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc ttatcgagcg
    15901 cgttggctac gttttccaga tgattacgct ggctggagat ataggcatct tccagcacca
    15961 ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag cagagattaa
    16021 cgacatgacc gggccccagg cggcgtgcga gttttttcaa ccagctctgg ttttccattt
    16081 ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct ggcataaccc
    16141 ggcaatagcg t
//
LOCUS       DAJTXY010000088        16071 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTXY010000088 DAJTXY010000000
VERSION     DAJTXY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.088"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(273..977)
                     /locus_tag="OO566_002868"
     CDS             complement(273..977)
                     /locus_tag="OO566_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6606085.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1114..1566)
                     /gene="moaE"
                     /locus_tag="OO566_002869"
     CDS             complement(1114..1566)
                     /gene="moaE"
                     /locus_tag="OO566_002869"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6606086.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1568..1813)
                     /gene="moaD"
                     /locus_tag="OO566_002870"
     CDS             complement(1568..1813)
                     /gene="moaD"
                     /locus_tag="OO566_002870"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6606087.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1806..2291)
                     /gene="moaC"
                     /locus_tag="OO566_002871"
     CDS             complement(1806..2291)
                     /gene="moaC"
                     /locus_tag="OO566_002871"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6606088.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2294..2806)
                     /gene="moaB"
                     /locus_tag="OO566_002872"
     CDS             complement(2294..2806)
                     /gene="moaB"
                     /locus_tag="OO566_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6606089.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2828..3817)
                     /gene="moaA"
                     /locus_tag="OO566_002873"
     CDS             complement(2828..3817)
                     /gene="moaA"
                     /locus_tag="OO566_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6606090.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3805..3949)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4214..5122
                     /gene="yvcK"
                     /locus_tag="OO566_002874"
     CDS             4214..5122
                     /gene="yvcK"
                     /locus_tag="OO566_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6606091.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5314..7335)
                     /gene="uvrB"
                     /locus_tag="OO566_002875"
     CDS             complement(5314..7335)
                     /gene="uvrB"
                     /locus_tag="OO566_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6606092.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7914..8591)
                     /gene="bioD"
                     /locus_tag="OO566_002876"
     CDS             complement(7914..8591)
                     /gene="bioD"
                     /locus_tag="OO566_002876"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6606093.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8584..9339)
                     /gene="bioC"
                     /locus_tag="OO566_002877"
     CDS             complement(8584..9339)
                     /gene="bioC"
                     /locus_tag="OO566_002877"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6606094.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9326..10480)
                     /gene="bioF"
                     /locus_tag="OO566_002878"
     CDS             complement(9326..10480)
                     /gene="bioF"
                     /locus_tag="OO566_002878"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6606095.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10477..11517)
                     /gene="bioB"
                     /locus_tag="OO566_002879"
     CDS             complement(10477..11517)
                     /gene="bioB"
                     /locus_tag="OO566_002879"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6606096.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11604..12893
                     /gene="bioA"
                     /locus_tag="OO566_002880"
     CDS             11604..12893
                     /gene="bioA"
                     /locus_tag="OO566_002880"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6606097.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12871..13341
                     /locus_tag="OO566_002881"
     CDS             12871..13341
                     /locus_tag="OO566_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6606098.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13895..14896
                     /locus_tag="OO566_002882"
     CDS             13895..14896
                     /locus_tag="OO566_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6606099.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14930..>16071)
                     /locus_tag="OO566_002883"
     CDS             complement(14930..>16071)
                     /locus_tag="OO566_002883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR6606100.1"
                     /translation="YDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFD
                     NVMVNEVETAYLMQGLWHSKFIACQAGTCRVGLHFLGQCVSVSVSSCHFSRGNYSADE
                     SFGIRIQPQTYAWSSEAVRSEAIILDSETMCIGFKNAVYVHDCLDLHMEQLDLDYCGS
                     TGVVIENVNGGFSFSNSWIAADADGTEQFTGIYFRTPTSTQSHKIVSGVHINTANKNT
                     AANNQSIAIEQSAIFVFVSGCTLTGDEWAVNIVDINECVSFDKCIFNKPLRYLRSGGV
                     SVTDCYLAGITEVQKPEGRYNTYRGCSGVPSVNGIINVPVAVGATSGSAAIPNPGNLT
                     YRVRSLFGDPASSGDKVSVSVVTINVTRPSPVGVALPSMVEYLAI"
BASE COUNT         3819 a         4231 c         3998 g         4023 t
ORIGIN      
        1 ataccgttta atatcctggc ctcgtgctga aaatctaatt tattgctgtt ttgcgagtat
       61 gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca ataaatttaa
      121 aaccacgcat acaaaatact tttgttttta tggatacgga aaacgattcc gctcgctcat
      181 aaggcatatt ttatacctgg tacttgttaa gtgtcgtttt tagtaagaaa aaaatcccga
      241 cacatggccg ggatagtgat ggtgaaaaag aattaacggc ggttgccgaa gatccgtaac
      301 aacatcagga acaggttgat gaagtccaga tacaaggtta acgcgccaag aatggaatat
      361 ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac ccatattttt caatttctga
      421 gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac tgcccacatc
      481 aatgcttcgc ttttcagcca gaagttgacc agcgatgcca gcacaatgcc gattaacgcc
      541 ataaacagca tattgcccaa gccacttaaa tcgcgcttcg tggtgtaacc gtacaggctc
      601 attgcgccga acatcccggc agtaacgacg aaagtactgg cgatagaagc agcggtatag
      661 acaatgaata tactggaaag cgtaagaccc gtcagcgccg aataaagcat aaagagcatc
      721 gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac caatgctaat
      781 tgcgcgatga tcagaccgat taaaaagaca cggttagtga acaacagctc catcacggcc
      841 gcggaattag ccgcatacca ggcaacaaat gcggtcagca acaagccaac ggtcatccag
      901 ccatagactt gcgccatata agtttgcaag ccagcccggg gttgtacgat tgaatcagaa
      961 cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa ggatactgca
     1021 aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt gaaattgccg
     1081 gacgcagcgg tgccttatcc ggctaacaaa aaactaccag cgttttgccg cctgcagatc
     1141 gctctcccgg gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct tccagaacgg
     1201 tgcgcgggtt ttgagataat ccataataaa ctgcccggct tcaaacgcac tgctgcgatg
     1261 cgcactggtg acaccgacaa aaacgatttc atcgcccggc cataattccc cgatgcggtg
     1321 aatcaccgtg acgcgcccca gcggccagcg gttacgcgct tcatcaacaa tttctgccag
     1381 tgctttttca gtcatccccg gatagtgttc gagggttaat gctttgacgc tgtcgcccag
     1441 gttatggttg cgcacctttc cggtgaaggt gactaccgca ccgtcttcgt cacgctccgc
     1501 cagccacggg tactcttctc ctacgctgaa cggctgcggg ccgacaacaa ttttggtttc
     1561 tgccatctta acctccggtt accggcggga ggaaagctac ttcgtcgccg tcagtcagcg
     1621 gatggtcaaa actcaccagc gtctggttga cggcagccag caatttgcca tcttccagcg
     1681 ccagcgccca gcgatcgctc tgcgcagcca ggtgctggcg taacgcttca acggttgggt
     1741 aatccgcagc cacttcggtt gcatctgttc ccaccaactc gcgcacctgg gcgaaaaaaa
     1801 gaactttaat catccgcttc caccttaaag tcacccgact tgccgccgct cttcgccagc
     1861 aaacgtaccg gaccaatcac catatctttt tgcaccgctt tgcacatgtc ataaatggtc
     1921 agcgccgcca cggaggccgc ggttaacgct tccatttcga caccggtttt cccggtcagg
     1981 cggcataagg tttctatacg cacccgattg tgctccggct cggcctgtaa attgacttca
     2041 actttgctga gcatcagcgg gtgacacagc gggatcagat cccaagtgcg ttttgccgcc
     2101 tgaataccgg caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg accatcaata
     2161 atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc ttcacgcacg
     2221 gtttccgctt tggcggagac atccaccatg tgcgcttcgc cagcggcgtt gatatgggtc
     2281 agttgcgaca tacttatttc ttcaaatgtg gatggaaatt acacggacgc gtacgggcat
     2341 ccagctgcgg cgcgatgata ttttcccatg cggtacggca cgctttggtc gaacccggca
     2401 tggcgaaaat cagcgttttg ttagcgacgc ccgctaccgc acgagattgc aacgtggaag
     2461 tgccaatctc ttcaaacgac aacatacgga acacttcacc aaaaccttca acctcacggt
     2521 cgaacaacgg cagcagcgct tcgggagcct gatcaccttc cgtcaggcca gtaccacccg
     2581 taatcaatac cacttgtaca tcgtcgctgg cgatccacgc agatacctga gcgcgaatag
     2641 cgtagcggtt ttctttcaca atggctttat cgacaacgtg atggcccgct tcttgcgccg
     2701 aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc gcgacgatta gaaaccgtaa
     2761 gaatagcaat acgggtcggg ataaattcag tgcttacctg actcatctga tctctccttt
     2821 tgacgtttta gccgccaatg tacgataagt tttgcgtaat accggtgttg ttttgatgca
     2881 ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc agcgcctgtt
     2941 gctgggtatc gtcttccagc agatcgcgca ggttaacgcc gccttcacca aacaggcaga
     3001 gatggagttt accaatggag gaaacgcgca ggcggttgca agtggcgcag aagtcttttt
     3061 catacggcat gataaggcca atctctccgg cgtaatctgg atggcaaaag acttgcgcgg
     3121 gaccgtcgct gcgttgacgt aattggtgga tccagccgcg acgcagtagc tcgtcacgca
     3181 gaacctgacc agagatgtga tgcttacgga agagctcgct gccctcgccc gtttccatca
     3241 gttcgatgaa acgcagctgg atagggcgat gctggatcca gttcagaaag gtgtcgagct
     3301 ggtgatgatt aacatcacgc atcagcacgg tattgacttt gaccttctca aaaccggcct
     3361 caaatgcagc atcaatccct gccatgacct ggttgaattt atcctgcccg gtaatagcgt
     3421 gaaactggcg ggcgtccaaa ctgtcgacac tgacgttaat gccagtaagt cccgcatcgc
     3481 gccagttcgc cacatcgcgt tccagacggt aaccattggt ggtgaccgca atctggcgga
     3541 tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt aaagacggct
     3601 ctcctcctgt caggcgcact ttttcggtgc ccaggctggc gaaggcgcgc gtaacccggc
     3661 gaatttcatc gacggtaaga aagcctttat tggtgacgcc gctcggtttg tagccatccg
     3721 gcaggcagta ggtgcaacga aagttacaca catcggtaat cgacaggcgc aagtagtaaa
     3781 acttacgcgc aaatgcatca gtcagttgtg aagccatgta cacctttcca gatacgggag
     3841 gcgaagtcat ttcttccttc gccctggtgg caatctttac gagcattgtc acggccaaag
     3901 caccgtatca gttgacccag gtgcagaggc tagagtgttt agtggttaag ccgatactag
     3961 cgtgtaaatg agatttttac catccacact ttcgctatgc aatcatgtat atagcgtcat
     4021 gatcgtgcta tttcgctaca gaataaggga aaatggcggt tttactttga tatacatcat
     4081 cagggaggca tagcgtgcat cgtcctttag agatagaaga aaatagtttg tatcctcaat
     4141 attggcaggt taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg ataatctgac
     4201 tccaggaaac tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg ttgctctcgg
     4261 cggagggcat ggactgggac gcgttctctc atcactttcg tctttgggtt ctcgtttaac
     4321 gggtatcgtc accaccacgg ataatggtgg ctcgacgggg cgtattcgcc gttcagaagg
     4381 cggcattgct tggggcgata tgcgcaactg cctcaaccag ctgataacgg aaccgagcgt
     4441 cgcctccgcg atgtttgaat accgttttgg cggcaatggc gaactttccg gtcataatct
     4501 cggaaacttg atgttaaagg cgctggatca ccttagcgtg cggcctctgg aagccatcaa
     4561 tttaattcgt aatctgctga aagtggatac gcatttgatt ccaatgtcag agcatcctgt
     4621 tgatctgatg gcgattgacg atcaggggca tgaagtttac ggcgaggtca atatcgacca
     4681 gttaactacg ccgattcaag agttattgtt aacgcctaat gtacccgcaa cgcgtgaggc
     4741 ggttcacgct atcaatgaag cggatctcat cattattggg cctggcagtt tttataccag
     4801 cctgatgcca attctgctgc tgaaggaaat cgcccaggca ttacgccgca cgccagcgcc
     4861 gatggtttat atcggcaatc tggggcgtga gttgagttta cctgcggcta atttgaagct
     4921 ggaaagcaag ctggcaatta tggagcagta tgttggtaaa aaggtcattg atgcggtcat
     4981 cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg attgtgatcc aggaggtact
     5041 ggaggccagc gatattccgt atcgtcatga ccgccagttg ttacataacg cgctggaaaa
     5101 ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc ctattgaaga
     5161 caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccgc
     5221 gcaaattcaa tatattgcgg agaaaatgta ggcctgataa gcgtagcgca tcaggctgtt
     5281 ttccgtttgt catcagtctt cttcgctatc ctgttacgat gcggcaataa acaactcacg
     5341 caactgatgc aactggtcac gaatttgtgc cgcttcttcg aactccagat tctgcgcgtg
     5401 ttgcatcatc agcccttcca gttcatggat tttctgctgc aacgctttcg gcgacatatc
     5461 catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct tcgctttggt
     5521 tttggcaatg ttctgcccca gcgccaggat atcgaccact ttcttgttca agccttgcgg
     5581 cgtaatgccg tgttcctcgt tgtacttctg ctgtttctca cggcgacgtt cggtttcgcc
     5641 aatcgctttc gccattgatg gagtgatctt atcgccgtag agaatcgctt taccgttaac
     5701 gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca ggaagccttc
     5761 tttgtcagcg tcgaggatcg ccaccagcga cacttccggc atatccagac cttcacgcag
     5821 taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga taatctccat
     5881 acgctcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt gttcttcgag
     5941 atattcggta agatcttccg ccattcgctt ggtcagtgtg gtgaccagca cgcgttcgtt
     6001 aattgccgct cgctgacgaa tctccgaaag aagatcatca acctgtgtcg ccaccggccg
     6061 cacttcgata atcgggtcaa gcaatccggt tggacgcacc acctgatcca ccacatcgcc
     6121 gccggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg tttgcggcgc
     6181 taatgcttcg aactcttcaa acttaagcgg acggttatcc agcgctgatg gcaggcggaa
     6241 gccgtactcc accagtgtct ctttacgcgc ccggtcaccg cgatacatgc cgccaatttg
     6301 tggaatggtg acgtgagatt catcgacgac cagcagccca tcggcaggca ggtaatcaaa
     6361 cagcgtcggc ggtggctcac ccggtccacg accggagagg aagcgcgagt agttttcaat
     6421 ccccgaacag tagcccagct cgttcatcat ctccagatca aactgggtac gctgggtcag
     6481 ccgctgctct tccagcagtt tgttgttttc caacagcact ttgcgtctgg cggccagttc
     6541 ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt agtgcgtttt
     6601 cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg ggtcaaataa
     6661 cgacaatcgt tccacttcct cgtcaaacag ttccacgcga agtgcaatgt catccgattc
     6721 tgccgggaag atatctatca cctcgccacg aacgcggaaa gtaccacgct ggaatgcttg
     6781 atcattacga gcgtattgca gctccgccag tcggcgcaga atcgcgcgct gatcgataat
     6841 cataccgacc gtgagatgga gcatcatctt gagatataaa tcaggatcgc ccagaccata
     6901 aatcgcggaa acagacgcca ctacaatcac atcacgccgc tccagcatcg ctttggtggc
     6961 ggacaaacgc atctgctcaa tatgttcgtt aaccgaggca tctttctcaa tgaaggtgtc
     7021 ggaactcggt acataggctt ccggctgata gtagtcgtag taggagacga aatactccac
     7081 cgcgttttcc gggaagaact ctttcatttc gccatacagc tgggccgcca gcgttttgtt
     7141 gggcgcaagt accatggttg ggcgctgaag gtcagcaatg acattggcaa tggtgaaggt
     7201 tttcccggag ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt ccagcccctc
     7261 ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg aattcagttt
     7321 gaacggttta ctcatgagtc gctacctgaa ggagttgggc gggcaggtat gtaattttac
     7381 tcgtcgtact taattatgcc aacaaattat actggataaa aaaacagttc atcaccataa
     7441 tatttctgat acagcgtaaa ctccgctgtc aatcatgagc aaaatttact ctgtggcgag
     7501 ataaaactcc ggccttaccg ggttatcccc aaagcaacgg cttttttaac atttgtcaag
     7561 atgagtgatg acagttttat ggcaagagat gcctgtacag tgactgccat tgcatttatc
     7621 taaccagtta aaaaataaaa gatatttctt tgagccgtct ttaacgccaa ttcgcgtgca
     7681 agccgcgtat tctctcgctt gcctcgtgtt ttctaactct aatacacatg gttatccaca
     7741 ggaatagtgg ataactgtct ccagccccta tcccccgccg cctgggcaat tcccacattc
     7801 gccgcatgag gcggaagtaa aatttttttc gtgattattt tagaatttaa ttggttaaat
     7861 tgcagtcaat cgaagacgca atctcgctcg caatttaacc aaatacagaa tggctacaac
     7921 aaggcaaggt ttatgtactt tccggttgcc gcattttctg gattttctgc aagccagggg
     7981 atctctccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata ttcagcgtga
     8041 cgttttcccg gaggcgtaac atcgttcgcc acccaacccg ccagagtcag tccggcgtgt
     8101 tgtattacct gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac accaactacc
     8161 agtatcaccg gcagttgttc ctgtgttacc caatctgcaa aagtgaaagt gtcagaaagc
     8221 ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg ttcaagcgcg
     8281 cgtaatccgg cgctcattac caatgattct atcggcctgc cctcttgggc gctgatgatg
     8341 tgcggcgagg tgggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc cagctgcagg
     8401 ctgctgttgt gctgtaacgc cagcgcgtca ctattacgta agccttccgg ggtcttttcg
     8461 ctgccagagg cgaccggttt ataacctgcc gtccgataac ctactgcctt cgcggcttgt
     8521 aaaagtgcac aactggcgac ggttttcccc acttcggtat ccgttccggt gacaaaataa
     8581 cgtttactca cgagcaatca ctcccaaaaa aagatgatac gtcagaggat atcgcccctg
     8641 ttgttgcggc caggccagtt gcaatcgctg caactgcgaa cgcgttaata ttcgcgggtc
     8701 gcgcccttca tgaagatgcg tagcaccgat gcctttcagc gaacgcatgg cactgagcgc
     8761 atcatcaaac catagtgtga tgagctgaat atgatgttga taacgcacgc cgttcagcga
     8821 ctcttcgatt tcatctggcg gtaaaaagcg attagcatgc ggacgctcgt ccaccgcctg
     8881 ccgcgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg cgaccacgcc
     8941 tccggggcgc accacccgat acagctcgcg gagtgccgtg gataaattac cgcaccactg
     9001 caccgcgaga ttgctccatg caagatcgaa cgtcgcagtc gctaacggca gggattcgat
     9061 atctcccgcc agataatgat ctgcggcatc tttatggcgg gcttgcgcaa gcattggcgg
     9121 cgagagatct aacgccgtca cctgcgcgtg acgttcccgc cagtggcggc tcatccagcc
     9181 aggtccacaa cccgcgtcca gtacgtgggt gtatttacgc tgtggaagca ttgccagtaa
     9241 ggcgtcagca ctctggcgct gtagatctgc atgttgctca tagtgtgcgg ctgcccgacc
     9301 aaatgccgct gcaatggctt gtttattaac cgttgccatg cagcacctcc agcagacggt
     9361 cgatatcctg catttcatgc gcagcggtta gcgttaagcg aagtcgcgca gtaccagcgg
     9421 gtacggttgg cgggcgaatc gccgtgaccc agcagccttg ctggcgcagt ttttctgcca
     9481 gttgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgctg catgaatcag
     9541 ccagcgtaaa cggcaaatcc tgtactccgg cacgaaaacg cgcaatgagt gacaccagtt
     9601 tttcgcgccg tgcatccccc tcatcacggc gaatgaccgc cagcgacgca cgtaatgcct
     9661 gcgcctgagc gggcggcata ctggtgctgt agataaggtg gcgggcgaat tgcagcagat
     9721 aatccgccac cgtacttgag caaagcaccg cagccccact gacgccaaat cctttgccaa
     9781 aagtcactac cagcaattct ggttttacct tttgcagcca gcagctgccg cgcccctgct
     9841 ccccgataac gcccgtgccg tgggcatcat cgaccatcaa ccagccattg tgctgttgcg
     9901 ttacctgctg gatttccgcc agtggcgcgc tatcgccgtc catgctgaac acgccttctg
     9961 tcaccaccag ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga tgagcgacat
    10021 cgttatgagt aaaacggcga agctgcgacg ggcttaaact ggcagcctcc agcaatgagg
    10081 catggctaag ccggtcggca acaatacggt cctctttcgc catcatcgcg gcaatgactg
    10141 cctgattcgc ggcaaaacca gagataaaca gcagcgcccg cgaatagcca agccactcgg
    10201 ccagttcttc ttccagcgcc tgatgcacca cgctataacc gctgacgtga ccggagccgc
    10261 cgctaccgat gccaaattgc tccgccccct gcttccaggc acggataatt tgcggatgat
    10321 ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcgatca tccgccacca
    10381 gccagcgtcc ggctccttgc gccaccggat aacggcgacg cagggcatcg gcagcacgcc
    10441 gcgcatcgag cgccgcgttg attttctcct gccagctcat aatgctgccg cgttgtaata
    10501 ttcgtcggtg tccggggtca tcagcgcctg tccaagacgt tgctgttgtt cgttatcccc
    10561 tgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt gcaggtcttt
    10621 atcttcttcc ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg agtttgcgcc
    10681 tgccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg cagaaaggcg
    10741 cacgtaagag gtcggcatca tgatccgcgc tacggcaatg gtgcgaataa aatcaaaggc
    10801 atcaacgtca tcgttatcgg caagcggcgt gcctttcacc ttcaccagca tgttgattgg
    10861 tacgctttcc ggcggcgtcg gcaggtttgc cagttgcagc aataatccgg cgcgatcttt
    10921 taccgtttcg cctaagccca caatgccacc tgagcagact ttgatcccgg catcgcgcac
    10981 tttttccagc gtatcgaggc gttcctgata agtgcgtgtg gtgatgatat tgccgtaaaa
    11041 ctccggcgag gtgtccaggt tgtggttgta gtaatccagc ccggcgttcg cgaggcgctg
    11101 cgcctgagat tcactcaacg tgcccagcgt catacacgcc tccagcccca tcgcttttac
    11161 cccctgcacc atttgttcca ggtacggcat atcgcgttcg tggggattct tccatgccgc
    11221 gcccatacag aagcgcgtcg atcctgccgc tttcgctttg cgcgccgact ccagcacctg
    11281 ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc tttgcgggca
    11341 gtatttgcaa tcttccggac aagctccggt cttaatcgac agcaacgtgc tgacctgcac
    11401 ctgacgagga tcgaaatgct ggcgatgcac ctgctgcgct tcaaacagca gatccagcaa
    11461 cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt gagccatggg
    11521 gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag gcttgtaaac ctaaatcttt
    11581 tcaatttggt ttacaagtcg attatgacaa cggacgatct tgcctttgac caacgccata
    11641 tctggcaccc atacacatcc atgacctccc ctctgccggt ttatccggtg gcgagcgccg
    11701 aaggttgcga gctaattttg tctgacggca gacgcctggt tgacggtatg tcgtcctggt
    11761 gggcggcgat ccacggctac aatcacccgc agcttaatgc ggcgatgaag acgcaaattg
    11821 atgccatgtc gcatgtgatg tttggcggta tcacccatgc gcccgccatc gagctatgcc
    11881 gcaaactggt ggcaatgacg ccgcaaccgc tggagtgcgt ttttctcgcg gactccggtt
    11941 ccgtagcggt ggaagtggcg atgaaaatgg cgttgcagta ctggcaagcc aaaggcgaag
    12001 cgcgccagcg ttttctgacc ttccgcaatg gttatcatgg cgataccttt ggcgcgatgt
    12061 cggtgtgcga tccggacaac tcaatgcaca gcctgtggaa aggctatctg ccagaaaact
    12121 tgtttgctcc cgccccgcaa agccgcatgg atggtgaatg ggatgaacgc gatatggttg
    12181 gttttgcccg cctgatggcg gcgcatcgtc atgaaatcgc agcggtgatc attgagccga
    12241 ttgtccaggg cgcaggcggg atgcgcatgt accatccgga atggttaaaa cgaatccgca
    12301 aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc ggatttggtc
    12361 gtaccggcaa actgtttgcc tgtgaatatg cagaaatcgc gccggacatt ttgtgcctcg
    12421 gtaaagcctt aaccggcggc acaatgaccc tttccgccac acttaccacg cgcgaggttg
    12481 cagaaaccat cagtaacggc gaagccggct gctttatgca tgggccaact tttatgggca
    12541 atccgctggc ctgcgcggca gcaaacgcca gcctggcgat tatcgaatcc ggcgaatggc
    12601 agcagcaggt ggcggctatt gaagtgcagc tgcgcgagca actggcacca gcccgtgatg
    12661 ccgaaatggt tgccgatgtg cgcgtactgg gggcaatcgg tgtggtcgaa accactcgtc
    12721 cggtgaatat ggcggcgctg caaaaattct ttgtcgaaca gggggtatgg atccggcctt
    12781 ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag ttgcagcgtc
    12841 tgaccgcagc ggttaaccgc gcggtacagg atgaaacatt tttttgccaa taatggcgat
    12901 aagttgccgc atcgtcatgt ctttaacggc atgggttacg atggcgataa tatttcaccg
    12961 catctggcgt gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac ctgctacgac
    13021 ccggatgcgc caaccggctc cggctggtgg cactgggtag ttgttaattt acccgctgat
    13081 acccgcgtat taccgcaagg gtttggctct ggtctggtag caatgccaga cggcgttttg
    13141 cagacgcgta ccgattttgg taaaaccgga tacgatggcg cagcgccgcc gaaaggcgaa
    13201 acccatcgct acatttttac cgttcacgcg ctggatgtag agcgtattga tgtcgatgaa
    13261 ggaggcagcg gcgcgatggt cgggtttaac gttcatttcc actctctggc aagcgcatcg
    13321 attaccgcga tgtttagtta atcactctgc cagatggcgc aatgccatct ggtatcactt
    13381 aaaggtatta aaaacaactt tttgtctttt taccttcccg tttcgctcaa gttagtataa
    13441 aaaagctgaa cgagaaacat aaaacgctgt aaatatcaat atgttatgaa agatttagtc
    13501 taaataatag actgcataat actacaaaac acaacatatc cagtcactat gaatcaacta
    13561 cttagatagt attagtgacc tgaggcagag cattagcgca aggtgatttt ttgtcctctt
    13621 gcgctaattt tttgtcaacg gactgggtta gttgaattta caaccatgct aagtatctcg
    13681 ataagcgcag agaaatgatg cagtggtggg cggattggct tgatgagaag gttaggtagg
    13741 caaatccatg cctgagcaga gccttgccct ctacctgagc ccatgctcat tctttggcct
    13801 caacgcgagc atgacctcac tggctggacc caaaatggac tatgacagaa agatctgata
    13861 tttttcctcg caaaaactta gaggctttat tttaatgcat aagagcaact gctttgacac
    13921 ggcaaggctt gtggcagcta tgatggttct ggtcagtcac cactacgcgc tctctgggca
    13981 gccggagccg tacctgttcg gctttgaaag tgctggcggc attgcagtta tcatattctt
    14041 ttcaatatca ggatatctga tttcaaaatc agctatcagg agtgattcct ttattgattt
    14101 catggctaaa agagcgagaa gaatattccc ggcgctggtc ccttgctcta tactgacata
    14161 ttttttattt ggatggatac ttaacgactt cagcgcagaa tattttagcc atgacattgt
    14221 caggaagacc ataagctcta tttttatgag tcaggctcca gatgctgaca ttacatcaca
    14281 tttaattcat gccggaatta acggcagttt gtggacccta ccgcttgagt tcctgtgtta
    14341 tataataacg ggagtagcag ttgccctact aaagaacgga aaagcattca tagttatact
    14401 gttggttttt gtttctcttt cattaattgg aagtgtgtca gaaaacaggg atgtaatgtt
    14461 ctctattccg ttatggctgt atcctttgcg tggactggcg ttcttttttg gtgccactat
    14521 ggcaatgtat gagaagtcat ggaatgtaag taatgttaaa attactgtag tttctttgct
    14581 tgctatgtac gcctacgcta gttatggcaa aggcattgat tatactatga cttgctatat
    14641 cctggtttca ttctcgacaa tagccatatg cacatccgtt ggcgacccac tggttaaggg
    14701 caggtttgat tactcgtatg gtgtgtacat ttatgcattc ccggttcagc aggttgttat
    14761 aaacacattg cacatgggtt tttacccgtc aatgttgtta agtgccgtga ctgttctttt
    14821 cttgtcccat ctttcatgga atctggttga aaaaagattc ctgacacgaa gttcgcctaa
    14881 attatccctg gattgaacaa taaaaggggc catatggccc ctaaaataat cagatggcca
    14941 gatactcaac cattgaaggc agcgcgactc ctactgggct tgggcgagta acattaattg
    15001 tcaccacgga aacactaacc ttgtcaccac ttgatgcagg gtcaccaaaa aggcttctta
    15061 ctctgtatgt caggttcccc gggttcggta tagctgcaga tccgcttgtg gcacctaccg
    15121 caactgggac attaatgatc ccatttacag acggtacgcc tgaacagcca cgatacgtat
    15181 tgtatctgcc ttccggtttc tgcacctcgg taatgccagc caaataacag tcagtaactg
    15241 acacgccacc gctacgaaga tagcgtagag gcttattgaa tatgcactta tcgaaagaaa
    15301 cacattcatt gatgtcgaca atatttacag cccattcatc accagttaac gtacaaccac
    15361 ttacaaagac gaagatcgcc gactgttcta tcgctatact ttgattgttt gcagccgtgt
    15421 ttttgtttgc agtgttgata tgaacaccac tgacaatttt atgtgactgc gttgaggtcg
    15481 gcgttctaaa atatatcccc gtaaattgtt cagtgccatc ggcatctgct gctatccatg
    15541 agtttgagaa agaaaatcct ccgtttacat tctctataac cacgcctgtt gaaccgcaat
    15601 aatctaaatc cagttgttcc atatgcaaat caaggcaatc atgaacatag acggcatttt
    15661 taaaaccaat gcacatggtc tcactgtcta aaattattgc ttctgaccta actgcctctg
    15721 acgaccacgc atacgtttga ggctgaatcc tgatgccaaa gctttcatca gcagaataat
    15781 ttcctctgct gaaatggcag gagctgacac taacacttac gcactggcct aaaaagtgaa
    15841 gaccgactct acaggttcca gcctgacatg ctataaattt tgagtgccag agcccttgca
    15901 ttaaataagc ggtttcaact tcattcacca ttacattatc aaatacccag ccataaacct
    15961 gtctgatgac agagtcttct gctgtaatgt ttgtgtcaat accattgcag gtgttattac
    16021 cagttactcg tatatttctc agataacggc cgatagtgtc tgaatcataa g
//
LOCUS       DAJTXY010000089        15932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTXY010000089 DAJTXY010000000
VERSION     DAJTXY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.089"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(6..1001)
                     /gene="yjfF"
                     /locus_tag="OO566_002884"
     CDS             complement(6..1001)
                     /gene="yjfF"
                     /locus_tag="OO566_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6606101.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(988..2010)
                     /locus_tag="OO566_002885"
     CDS             complement(988..2010)
                     /locus_tag="OO566_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6606102.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNLVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2024..3526)
                     /locus_tag="OO566_002886"
     CDS             complement(2024..3526)
                     /locus_tag="OO566_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6606103.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDMREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLNTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3666..4623)
                     /gene="ytfQ"
                     /locus_tag="OO566_002887"
                     /pseudo
     CDS             complement(3666..4623)
                     /gene="ytfQ"
                     /locus_tag="OO566_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4933..5463
                     /gene="ppa"
                     /locus_tag="OO566_002888"
     CDS             4933..5463
                     /gene="ppa"
                     /locus_tag="OO566_002888"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6606104.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5843..6184)
                     /locus_tag="OO566_002889"
     CDS             complement(5843..6184)
                     /locus_tag="OO566_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6606105.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6187..9966)
                     /gene="tamB"
                     /locus_tag="OO566_002890"
     CDS             complement(6187..9966)
                     /gene="tamB"
                     /locus_tag="OO566_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6606106.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9963..11696)
                     /gene="tamA"
                     /locus_tag="OO566_002891"
     CDS             complement(9963..11696)
                     /gene="tamA"
                     /locus_tag="OO566_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6606107.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11902..12540
                     /gene="msrA"
                     /locus_tag="OO566_002892"
     CDS             11902..12540
                     /gene="msrA"
                     /locus_tag="OO566_002892"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6606108.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12863..14206
                     /locus_tag="OO566_002893"
     CDS             12863..14206
                     /locus_tag="OO566_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6606109.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14268..14474)
                     /locus_tag="OO566_002894"
     CDS             complement(14268..14474)
                     /locus_tag="OO566_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6606110.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14799..15353
                     /locus_tag="OO566_002895"
     CDS             14799..15353
                     /locus_tag="OO566_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6606111.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPRSQFIVDSNGVARGAWQLDDESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15416..>15932)
                     /locus_tag="OO566_002896"
     CDS             complement(15416..>15932)
                     /locus_tag="OO566_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606112.1"
                     /translation="ILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRGKFE
                     LQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLEDEDY
                     SLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQLAL
                     PVLVRCKNSET"
BASE COUNT         3792 a         4368 c         3913 g         3859 t
ORIGIN      
        1 catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg attctcccac
       61 agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc gatggcgatt
      121 ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat cagtccctga
      181 attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa caacgtaccg
      241 ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag tgcatatccg
      301 gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt ggagagcata
      361 taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga cgtcgcgttg
      421 ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa aataccgata
      481 accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc ggggattttc
      541 cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga ctcttccgaa
      601 acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat aaatgccggg
      661 atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc catcaccagc
      721 actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt tgccagaaac
      781 acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga gaggatcaca
      841 aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat attgcagatc
      901 acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc cagcacaaag
      961 acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta ctcctttaat
     1021 cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca ccgctttcac
     1081 caccaggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca tcccctgaat
     1141 aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca gcgatccgcc
     1201 gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt tgttggcatc
     1261 ggcaccgcaa atatccgccg ccacgataat gcccgcaatc gccgcacata gcccgctcaa
     1321 cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg ccgcccgaat
     1381 gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca acagccagaa
     1441 caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca gcgatccgct
     1501 gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg tgatcagttg
     1561 cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct gaattttgag
     1621 gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca gggcgcttaa
     1681 caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg ctccggcgat
     1741 agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca gcgtcatacc
     1801 aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc taccgaacaa
     1861 acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat cgaccagcag
     1921 caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac gcctttttgg
     1981 cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc gcaatggcgt
     2041 tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt ttgcgatcgc
     2101 gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag gagatcacca
     2161 gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg gcgtgggcac
     2221 caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg gtcagtagcc
     2281 aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc ggttgttcag
     2341 ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct tgctgctctt
     2401 tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcaaggatg atattttccc
     2461 gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag aatccgatgc
     2521 ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg atcaacgccg
     2581 tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta cgtccggatc
     2641 ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca aacggtgcta
     2701 tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc aacaatgttc
     2761 gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt tttaccagtt
     2821 cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg cgtaagacgg
     2881 tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag atcaggctga
     2941 cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttct tgggtgttga
     3001 ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata gcccggcaaa
     3061 tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc atgtcgaggg
     3121 agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta cggcgtagaa
     3181 ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac atattgggta
     3241 gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg gcggtatttt
     3301 tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg tatacaccag
     3361 ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc gccatgattt
     3421 cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg aaaaatttac
     3481 ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt tcccccggtg
     3541 taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag gcttcagtgc
     3601 accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat caggcatagc
     3661 aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt atcaggcagg
     3721 taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt tttgtatttc
     3781 tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact ggcgttcgct
     3841 tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc cgtgaggata
     3901 tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat caccatgtcg
     3961 tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt gataaagctc
     4021 tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg gataattttg
     4081 atatttggcg cattcttaat ggcttcggca aagcctttct taacggtcaa tggcgacgct
     4141 ggccccaacg gtgccctgca gctccaccac gttgcatggt ttgccattca cttcttttac
     4201 cagccagtca ccgatcaact taccttcgag gatgttgtcg gcagtaacgg tggtcatata
     4261 gagagatttg tctttcacat cgatggaacg gtcgagcaag aagaccggga tttcggcatc
     4321 tttcgcctct tttaataccg gttcccaacc cgtcgcaacc actggagcga taaagatcgc
     4381 atccacccct tgtgcaacga aggaacgtac cgctttaatc tggttttcct gcttttgctg
     4441 accatcggca attttcaacg taattccgcg tttttcggcc tcacttttcg ccacattggt
     4501 ttctgcggcg cgccagccgg attccgatct gacctgcgaa aatcctacgg ttaatggagc
     4561 ggccaacgcc atagacgaca tggctgccga gactgcagag actacaagta agcgtttcca
     4621 catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc gaaaaaacta
     4681 tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt aaataaaacg
     4741 ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcta cggttatgga
     4801 ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag gaaaatgaag acgaaaacaa
     4861 gcaaagacat tcggcgcgag ttggctataa tacttggcac ttgtttgcca catattttta
     4921 aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc ggaagacatc
     4981 tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat cgacaaagag
     5041 agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc gtgcaactac
     5101 ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact ggtcccgact
     5161 ccgtacccgc tgcagcctgg ttctgtgatc cgttgccgtc cggttggcgt tctgaaaatg
     5221 accgacgaag ccggtgaaga tgcgaaactg gttgcggttc cgcacagcaa gctgagcaaa
     5281 gaatacgatc acattaaaga cgtaaacgat ctgcctgaac tgctgaaagc gcaaatcgct
     5341 cacttcttcg agcactacaa agacctcgaa aaaggcaagt gggtgaaagt tgaaggttgg
     5401 gaaaacgcag aagccgctaa agctgaaatc gttgcttcct tcgagcgcgc aaagaataaa
     5461 taagttcttc tagcgcaata accctgaacg ccgggcttcg gttagtaagg gtttttttat
     5521 gcccgcgata aataaactct ctattccacc atcattattc tcagcggttg caaggcttga
     5581 acggtaagaa caagcaaacc cgaccaccat tttgctgttc atagccactt gctggaagtt
     5641 agccgacctc actcatactc accgttcaaa gcgtgccccc gttttacccc tgcacatttg
     5701 tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg gagagaaaag
     5761 caaaagatgg aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa caacgccacc
     5821 cgaaggaggc gtgcttttat tattacttat ccctgtctaa ccagtcgccg ctttcaatta
     5881 gctttaatcc atcgacgggt cgttgataaa cgtacatcca tgcactccca tacggcgtct
     5941 gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt tcggccagcg
     6001 tggcgttgtc aatacgataa acttcaccgt gtaccgttcc gttccccgga actgcgcctg
     6061 gatagtggcc caggctatac aactggtagt tatcgatact gaaatcgccc agtaactggg
     6121 cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag acaaatattc
     6181 gcattgctaa aactcgaact gatagagcaa atccagtgcc tggtctacac cagacacggc
     6241 ttccagatat agcttaggca tcaggcgata acgtaacgtg agtgttgcta tagagtcaaa
     6301 tatacccacg ccgtatttca cttgcagacc tggcaataca tagccgctga ccactacctg
     6361 ggaggagtcg cctactccca gggtgtcgag cgctaaattg cttacgccaa acgtctcgcc
     6421 gattttaccc acaatctggc cactttgcgc aacccccaga ccaatcagca tcgaggtcat
     6481 tgccgcactg tcgctctgat cgctctccag cccttgtcca cgtagcaaat aagacaatgc
     6541 agcttgttgc gacatcgccg ggtcagagaa gatctccgct ttcggttcgt ccgccagacc
     6601 agtgacgcga actccggcga ttacatcgtc ttctgtagca tccgggttac gaatagcttc
     6661 aatattaaga tacggttgat ccggcggacc agagaacagt aactcaccct tacgcacaat
     6721 cagatcctga ccataggcat ggaagcgccc ttcagggatg ttgatctgcc cgttcaggcc
     6781 cagcccttgt ttgtcctgta cgacgttaag atcgcccgtc agccgcgctt tcaggccaaa
     6841 ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg ttactgttaa tcggaatcga
     6901 cgccgttttc ggctcttccg gttgcaggtt atcgttaagc atcaccacat cgctggagac
     6961 gcctactgcg ctttccggca gatcgtgcac cacgatgcgc gcccacggga catccacgcg
     7021 accatcgagg gtaaacaggt ttggtgtagc ctcgaataca acatctggcg atacatccat
     7081 tcgtaccatc ggcggcacgg tgatccgcac tttactgcct ttcgccgtta ctcgtgcccg
     7141 ccagttttca atttggctcc agtcggcatc accgttcagg tagatttcac cctgctgggt
     7201 ccgtactgta ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa gctggctcgg
     7261 ctgcatatca aacggcataa agttgccgtc gatatccaca cccgtaacct gaagctgacc
     7321 aaacaactgc gggctttgca catcaccacc cagacgcaag ttggcactca ccatccccgc
     7381 tgctttttcc ccacgggtaa agatggggtt tatcatcgca aggttgaagt tacggatatt
     7441 gacgttgcca ccaagattac ggcggccttg cggatcggtc acctgcacct gtccatcaaa
     7501 ctggccgtta ttggtcaggc ggatggtcca gcccaattcg gcacggttgt tacgcaattc
     7561 cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga cggtttgcgt
     7621 tacctgcacg ttacgccccg aaagggtgat actgccctgc ggcagcccct ctttcgtggt
     7681 gtcccaggca acatccgctt tacccgtgaa gataccgctg gcctgagtgg tttctggcat
     7741 aaacggtttc agcatggcga ggtcgaagcg gttgagattc accaccgcac gcccttcggc
     7801 ccccgcatcg atagtttgcg gcacgcacag ttccgcattc gggttaagcc agcagtgtgg
     7861 cccgatgctg attttttgct ccttattgcg gtaatccagc gcaatatcgc gggtcagcga
     7921 ccacgggccg accggcgtct ggaagcgggt attgctaaga gttcccttcc agcgttcttc
     7981 tttgcgatca aaacttcctg ccagattaag ctgcccggag acaggctcgc cctgaatccg
     8041 caactgtagc tcgtgctgct tttcgctgcc tttggcattc agggtgacga ggttgatatt
     8101 tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga tctgatcggt
     8161 ggatttaatg tcgccttcca cgcgaacctg cgccacggaa agttcctgcc agcgcaggcc
     8221 gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc gtgccgcgta ctttcaccag
     8281 ccctttcgct gtaccgccaa gccccggcag cgcgttatcc agccccggcg cgttgatggt
     8341 ggcatcaaga ttgagatctt ttacccccag ctcgcctttc acttcggcac tgtttggccc
     8401 gagttccaga tgaagcccag ggatcatcca ctgcatataa ctgttgcctt tcagcgtgcc
     8461 gtcaacgttc actttgttct gtttgacgtt accggtcagc ttcagttctg gcacctccat
     8521 ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc aagccattga gtttcgacgg
     8581 ccactccggg aactctttgg cggtgtaaat gccattaagc gttagctcac cgcgccaact
     8641 aatggcctgc tgccagtcga gcaacgcctt gagttcagtt ttcccttcca gcgccgcgac
     8701 ggtgagtttg tcgagattga cctgctgttc attacctttg gcatcaaggg taatggtggc
     8761 tggcgggatc tcctgtccct tcactgccgt acgcatagag agcgtgtaat cggtcatttt
     8821 gccggtaagt ttcagtttca gatcatccgc ctgatactgc ttctcaccag tgaacggcca
     8881 gtaaagctgt ttgctgttca cttccacgtt gagtggcaat ccggcttccg ccagtcgcgt
     8941 ctgggcgcgt aaatccatat ccaccggacc ggaaaggtta acgcctatct ccagctgttc
     9001 gcgcagcgcg ccgcccactt tcagcttcac cttttcacct ttcaacggct ccacgttcag
     9061 tgtgctgttg agggtgatat ccaccggcca gttgtctgac agctgcgccg tgccgctggc
     9121 gttgacgatc ccctggttgg aatcgatatc caaggcgtcc agtttagtat tgccgtcaat
     9181 gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg tcacgcgcag
     9241 ctgctcgccc ttaaactctt caatgttcag gttaagcggc aaatgcacgt cggtcatttc
     9301 cggcaatacc gggcgagaaa agagatcttt cagcgtttcg ccgagcggct tttcctccgg
     9361 ctgcggattt tcaattttcg gttcgaccac ttcttcctgc gccacttcca ccactttcgg
     9421 cagagcaatc agcaggcctt tcagcgacgt cggtttcagg gtcagggttt tctcctgcca
     9481 gttcaggccg gaggtgaagt ccatcaccga caccgtggtg tcatcaatct tgatgttgac
     9541 gttgtccagc gccacccgtg tcagggtgat gggatacggc gtggagagat ccagcggacc
     9601 gctatcttcc tcttcttcaa cctgttcaga aggaggcatt tttttactgt cgatgttgac
     9661 ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta ctgttccaca ggcactcaag
     9721 accgaccgcc agatgcagat tacccgcttt taccgccacg cctagctgct cataacgaac
     9781 gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca gtcctggcac
     9841 ccagcgatcc gccgctttaa atacaagatg caggccgctg gtggtgccca ccagaaacgc
     9901 caccgatccc agcaacagta agataacgat aaccacgccg aggctgattt ttttccataa
     9961 actcataatt ctggccccag accgatgtaa aactgtaacc cgtgttcgtc tttatccgcg
    10021 accggtacgg caaaatcgag tttgattggc ccgaccggcg attcccagcg tacgccgacc
    10081 ccggtaccgg ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc actatcgaca
    10141 aacaccgcgc cccaccattt tccggtcacg ttgtactggt attccagcga tccggttatc
    10201 aacttcgagg cccctttcag gtcaccgttg gcgtatttcg gggcgataga tttgtatttg
    10261 tagccgcgaa tactgcgatc gcccccgacg aagaaacgca gatccggcgg tactttgtcg
    10321 aaatcaccgg tttcaatcca gcccagcgta ccgcgtgtca caaagcgatg gcgatcgtac
    10381 agtgtgcgga tccagacgtt ctgcgcctgg aaaacggaga aatcgacatc tgaaccccag
    10441 gccgtgttgg agtagtcgat agagtagcgt tgcgagtctc cccaggttgg catcaggcca
    10501 ccacgagaac gcgtgcggct aatcatcacc ccaggataaa acagcatcgt ggtgttggta
    10561 atttcaccct gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg ctgccagccg
    10621 ctggagagat cccagtagcg agaagccacc agcgtagtgg agtcagattc ggtatcgttc
    10681 aggtcagtgc gcttaaaacc gccctgcacc aaataatatt gttccagtgg attcttcagc
    10741 agcggcattt tatagctgaa gtcgagggtc tgttccggcg cggaaatact ggtactggtg
    10801 gtcaaactgt gaccgtatga gttcatccac ggctttttcc acgtcgcttt cacgcgcggt
    10861 cccacgtccg tagagtaacc gcccccggtt tcgatggtgt tttctgttcg cggcgaaacc
    10921 acgcccgtca atggtaatac tttcgtttcg cgcgctttat caaattgtgg agccaccacc
    10981 accgagttaa accagccggt agcagaaagt cggcggttca gttccgccaa atctttcgat
    11041 tcatactcat cgccctcttt aaacggcacc agattttgca ggtattcatc gcggatttgt
    11101 gatccttcaa aggtcacatg cccaaagcgg taacgttcgc cactgttata atcaatatcc
    11161 cagaaggctt tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa ttcgctatcg
    11221 aaataacctt tacgcaacgc aatactggtt aaggactttt tgaaattttc ataatcgccc
    11281 tggttcagta ccgtaccaat agccgggcga gtatcgagca atttcaaata gtctttatcg
    11341 gtccgcgcgc cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg cacacctggc
    11401 gtgactttgg cgatcaatac ctgccgccct ttctttggcg gtggacggag atcaaattca
    11461 atggtcggct ggtaataacc cagcgctttc agaccttcac ggatggcatc atcgacgcgt
    11521 gcgcgaaagc gacggtctgg cgtcacttca tcactttcaa tcgtagaaag ctgcgcacga
    11581 acgttctttt ccagctgtcc cgataacccc tcgacctgta gacggacgtt cgcggcgacg
    11641 gcagatccgc ttaagcagag taagcttaca cagcataact gtcggatata gcgcacattt
    11701 tctcctgaat atcctttttt cctgcccctg gaacgccgtt aaacggctta acaaaaatcc
    11761 agtaatatgg attaaaaagc agactaaacc ccaaatattt cttatgtttt actttagacc
    11821 tattcacggt ggttattgtg tgcaaatacg cctcttgtta caaccttaac cccaatgacc
    11881 gattttcggg agagcgacac catgagttta tttgataaaa agcatctggt ttcccccgcc
    11941 gatgccctgc ctggacgtaa caccccgatg cccgtagcca cgctgcatgc ggtcaacggt
    12001 cactcaatga ccaatgtacc tgacggaatg gagattgcca tttttgcgat gggttgtttc
    12061 tggggtgtgg agcgtctgtt ctggcagtta cccggccttt acagcaccgc cgcaggctat
    12121 accggcggct atacgccaaa tccgacttat cgggaagtgt gctccggtga tacgggtcat
    12181 gccgaagcgg tacgcattgt ttacgatcct tccgtcatca gctatgagca gttgctacag
    12241 gtattttggg agaatcacga tcccgcccag ggaatgcgtc agggcaatga ccacggcacg
    12301 cagtatcgtt cagcgattta tccgctgacc ccagaacagg atgccgcagc tcgcgccagt
    12361 ctggaacgtt ttcaggcggc gatgcttgcc gccgatgacg atcgtcacat caccacggaa
    12421 atcgctaacg ccacaccgtt ttattatgcc gaagatgacc accagcaata tctgcataaa
    12481 aacccatatg gttactgtgg aattggcgga attggcgtct gtctgccgcc ggaagcatag
    12541 cgttacgggt acaaatgtag tttgttgata aagtgctctt tgtctatgcc tgatgcggct
    12601 taaacgactt accaggccta caaaatcgtc caaattcaat atattgcaag gactgcgtag
    12661 gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga attttaaccc
    12721 tctggcgact ttacagtacc ttacgctata ctagcgactg aaaatgccgg ttcactttct
    12781 tcgaatcggc tttcaatgtg tatttcacac aaattaatca actttccctt ccgaggatct
    12841 ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct gcttgatcgc
    12901 tgtaagtgcg ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca aaatcaaact
    12961 taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata tgcaggaaaa
    13021 tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga ttctcggcgg
    13081 tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc gctatatgtc
    13141 ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga ctggcatgtt
    13201 tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag aagcggtggc
    13261 tttgcgtatc atcaacccga tgcgcttctg cctgtacgtt tgcaccccgc tggtgtggtt
    13321 cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg tacgtaaaga
    13381 tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg cgggcgtgtt
    13441 acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc gtaccgttcc
    13501 gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag atgagcaaag
    13561 cctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta atgaagatat
    13621 tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc tggctaacca
    13681 aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc cggatacgtt
    13741 aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg cggtgatcat
    13801 gaacgagtac gcgctggtgg tggggatcat caccctcaac gacgtgatga ccacgctgat
    13861 gggcgatctg gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg agaactcatg
    13921 gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata ttgacgagtt
    13981 cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc tgcgtaagat
    14041 cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg tggatatcga
    14101 taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca ccgccctttc
    14161 gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc cagaaacatc
    14221 aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta agccatctct
    14281 gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg cagcttatcc
    14341 ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac gtccttgatg
    14401 tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg gttgtaccgt
    14461 tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc cacaatcggc
    14521 ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc gggcaatgtt
    14581 tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta tctttttacc
    14641 gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct tgaccgcaaa
    14701 ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac tcagacccaa
    14761 gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc aagattctgg
    14821 cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa atcggtcagc
    14881 gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa gatcagttta
    14941 gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gcgagtactg caacatattg
    15001 ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt aaatcagcga
    15061 agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac gcgattccgg
    15121 gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat cccaggtcgc
    15181 agtttattgt cgatagcaat ggcgtcgcgc gcggtgcctg gcagctggat gacgaaagtt
    15241 ccgctgtagt ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac ggggcgctca
    15301 caccggaaga ggtgcagcaa gtgatggacc tgctgcaaaa gttgctcaaa taatggcaat
    15361 aacataaatt ctggaaaaat aattttcccc tatcgttatc tatgggatag gggaattaag
    15421 tctctgagtt cttacaccga actaatactg gtaacgctag ttgctctttt ttaaacgcct
    15481 caatgatcga tttatatttc tcgcaaatct tttctttggt aatattacta tctgcggcca
    15541 gattcgcact tactatcgca tccacttgtt cggctaattc gacttgttgg caacctgcat
    15601 taaccagaaa attataaata agagagtaat cttcatcctc caaaacaaca gaggaaccat
    15661 tacgaaaacg ttcatcccct ccatagattt taagcgcaaa gcccaaagat tctatgacta
    15721 tggcaaaatt atcatcaatt attttttcgt aaatatacca tccatcacat tctttaattc
    15781 caccgcctgg tatttggagt tcaaatttac cccgttttaa tttgcttact acatcctcca
    15841 ataaggcata accgaaagat acgtcaccaa acgcctggcg aatataacct ttaatccttt
    15901 ttggtatgtc agactgtttt ttctgcaaaa ta
//
LOCUS       DAJTXY010000090        15886 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTXY010000090 DAJTXY010000000
VERSION     DAJTXY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.090"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..378
                     /gene="tuf"
                     /locus_tag="OO566_002897"
     CDS             <1..378
                     /gene="tuf"
                     /locus_tag="OO566_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6606113.1"
                     /translation="RAGENVGILLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILS
                     KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLG"
     gene            561..755
                     /gene="bfd"
                     /locus_tag="OO566_002898"
     CDS             561..755
                     /gene="bfd"
                     /locus_tag="OO566_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6606114.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            828..1304
                     /gene="bfr"
                     /locus_tag="OO566_002899"
     CDS             828..1304
                     /gene="bfr"
                     /locus_tag="OO566_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6606115.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1301..1768)
                     /locus_tag="OO566_002900"
     CDS             complement(1301..1768)
                     /locus_tag="OO566_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6606116.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            2147..2458
                     /gene="rpsJ"
                     /locus_tag="OO566_002901"
     CDS             2147..2458
                     /gene="rpsJ"
                     /locus_tag="OO566_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6606117.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2491..3120
                     /gene="rplC"
                     /locus_tag="OO566_002902"
     CDS             2491..3120
                     /gene="rplC"
                     /locus_tag="OO566_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6606118.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3131..3736
                     /gene="rplD"
                     /locus_tag="OO566_002903"
     CDS             3131..3736
                     /gene="rplD"
                     /locus_tag="OO566_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6606119.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3733..4035
                     /gene="rplW"
                     /locus_tag="OO566_002904"
     CDS             3733..4035
                     /gene="rplW"
                     /locus_tag="OO566_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6606120.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4053..4874
                     /gene="rplB"
                     /locus_tag="OO566_002905"
     CDS             4053..4874
                     /gene="rplB"
                     /locus_tag="OO566_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6606121.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4891..5169
                     /gene="rpsS"
                     /locus_tag="OO566_002906"
     CDS             4891..5169
                     /gene="rpsS"
                     /locus_tag="OO566_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6606122.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5184..5516
                     /gene="rplV"
                     /locus_tag="OO566_002907"
     CDS             5184..5516
                     /gene="rplV"
                     /locus_tag="OO566_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6606123.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5534..6235
                     /gene="rpsC"
                     /locus_tag="OO566_002908"
     CDS             5534..6235
                     /gene="rpsC"
                     /locus_tag="OO566_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6606124.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6248..6658
                     /gene="rplP"
                     /locus_tag="OO566_002909"
     CDS             6248..6658
                     /gene="rplP"
                     /locus_tag="OO566_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6606125.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6658..6849
                     /gene="rpmC"
                     /locus_tag="OO566_002910"
     CDS             6658..6849
                     /gene="rpmC"
                     /locus_tag="OO566_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6606126.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6849..7103
                     /gene="rpsQ"
                     /locus_tag="OO566_002911"
     CDS             6849..7103
                     /gene="rpsQ"
                     /locus_tag="OO566_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6606127.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7268..7639
                     /gene="rplN"
                     /locus_tag="OO566_002912"
     CDS             7268..7639
                     /gene="rplN"
                     /locus_tag="OO566_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6606128.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7650..7964
                     /gene="rplX"
                     /locus_tag="OO566_002913"
     CDS             7650..7964
                     /gene="rplX"
                     /locus_tag="OO566_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6606129.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7979..8518
                     /gene="rplE"
                     /locus_tag="OO566_002914"
     CDS             7979..8518
                     /gene="rplE"
                     /locus_tag="OO566_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6606130.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8533..8838
                     /gene="rpsN"
                     /locus_tag="OO566_002915"
     CDS             8533..8838
                     /gene="rpsN"
                     /locus_tag="OO566_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6606131.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8872..9264
                     /gene="rpsH"
                     /locus_tag="OO566_002916"
     CDS             8872..9264
                     /gene="rpsH"
                     /locus_tag="OO566_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6606132.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9277..9810
                     /gene="rplF"
                     /locus_tag="OO566_002917"
     CDS             9277..9810
                     /gene="rplF"
                     /locus_tag="OO566_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6606133.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9820..10173
                     /gene="rplR"
                     /locus_tag="OO566_002918"
     CDS             9820..10173
                     /gene="rplR"
                     /locus_tag="OO566_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6606134.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10188..10691
                     /gene="rpsE"
                     /locus_tag="OO566_002919"
     CDS             10188..10691
                     /gene="rpsE"
                     /locus_tag="OO566_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6606135.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10695..10874
                     /gene="rpmD"
                     /locus_tag="OO566_002920"
     CDS             10695..10874
                     /gene="rpmD"
                     /locus_tag="OO566_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6606136.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10878..11312
                     /gene="rplO"
                     /locus_tag="OO566_002921"
     CDS             10878..11312
                     /gene="rplO"
                     /locus_tag="OO566_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6606137.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11320..12651
                     /gene="secY"
                     /locus_tag="OO566_002922"
     CDS             11320..12651
                     /gene="secY"
                     /locus_tag="OO566_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6606138.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12683..12799
                     /gene="rpmJ"
                     /locus_tag="OO566_002923"
     CDS             12683..12799
                     /gene="rpmJ"
                     /locus_tag="OO566_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6606139.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12946..13302
                     /gene="rpsM"
                     /locus_tag="OO566_002924"
     CDS             12946..13302
                     /gene="rpsM"
                     /locus_tag="OO566_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6606140.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13319..13708
                     /gene="rpsK"
                     /locus_tag="OO566_002925"
     CDS             13319..13708
                     /gene="rpsK"
                     /locus_tag="OO566_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6606141.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13742..14362
                     /gene="rpsD"
                     /locus_tag="OO566_002926"
     CDS             13742..14362
                     /gene="rpsD"
                     /locus_tag="OO566_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6606142.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14388..15377
                     /gene="rpoA"
                     /locus_tag="OO566_002927"
     CDS             14388..15377
                     /gene="rpoA"
                     /locus_tag="OO566_002927"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6606143.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15418..15801
                     /gene="rplQ"
                     /locus_tag="OO566_002928"
     CDS             15418..15801
                     /gene="rplQ"
                     /locus_tag="OO566_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6606144.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4087 a         3740 c         4234 g         3825 t
ORIGIN      
        1 cgtgctggtg agaacgtagg tattctgctg cgtggtatca aacgtgaaga aatcgaacgt
       61 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      121 tacattctgt ccaaagatga aggcggccgt catactccgt tcttcaaagg ctaccgtccg
      181 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      241 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc taatccaccc gatcgcgatg
      301 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgtt
      361 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
      421 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      481 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      541 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      601 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      661 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      721 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      781 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      841 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      901 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      961 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
     1021 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
     1081 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
     1141 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
     1201 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
     1261 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
     1321 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1381 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1441 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ccaggcgcca agggcggcga
     1501 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1561 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1621 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1681 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1741 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1801 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1861 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1921 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1981 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     2041 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     2101 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     2161 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     2221 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     2281 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     2341 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2401 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2461 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2521 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2581 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2641 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2701 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2761 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2821 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2881 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2941 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     3001 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     3061 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     3121 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     3181 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     3241 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     3301 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3361 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3421 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3481 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3541 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3601 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3661 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3721 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3781 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3841 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3901 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3961 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     4021 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     4081 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     4141 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     4201 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     4261 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     4321 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4381 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4441 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4501 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4561 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4621 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4681 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac tgcgatgaac ccggtagacc
     4741 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4801 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4861 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4921 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4981 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     5041 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     5101 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     5161 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     5221 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     5281 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     5341 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5401 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5461 cagatcgtat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5521 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5581 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5641 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5701 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5761 tatcggtaag aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5821 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5881 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5941 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     6001 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     6061 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     6121 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     6181 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     6241 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     6301 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6361 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6421 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6481 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6541 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6601 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6661 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6721 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6781 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6841 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6901 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6961 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     7021 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     7081 ttgtagagaa agcggttctg taatacagta cactctctcg atatgaataa acggctcaga
     7141 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     7201 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     7261 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     7321 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7381 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7441 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7501 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7561 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7621 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7681 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7741 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7801 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7861 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7921 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7981 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     8041 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     8101 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     8161 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     8221 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     8281 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     8341 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8401 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8461 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8521 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8581 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8641 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8701 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8761 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8821 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8881 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8941 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     9001 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     9061 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     9121 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     9181 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     9241 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     9301 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9361 aaaaacggcg agctgacccg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9421 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9481 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9541 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9601 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9661 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9721 cgcgcctacc gtcgtcctga gccttacaaa ggcaagggtg ttcgttacgc cgacgaagtc
     9781 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9841 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9901 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9961 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
    10021 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
    10081 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
    10141 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
    10201 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
    10261 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
    10321 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10381 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10441 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10501 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10561 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10621 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10681 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10741 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10801 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10861 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10921 cgggtaaacg cctggggcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10981 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    11041 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    11101 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    11161 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    11221 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    11281 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    11341 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11401 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11461 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11521 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11581 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11641 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11701 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11761 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11821 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11881 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11941 gagcaagcgc gtcaaggtga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    12001 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    12061 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    12121 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    12181 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    12241 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    12301 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12361 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12421 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12481 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12541 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12601 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12661 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12721 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12781 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12841 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12901 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12961 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    13021 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    13081 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    13141 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    13201 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    13261 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    13321 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13381 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13441 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13501 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13561 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13621 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13681 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13741 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13801 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13861 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13921 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13981 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    14041 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    14101 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    14161 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    14221 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    14281 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    14341 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14401 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14461 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14521 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14581 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14641 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14701 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14761 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14821 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14881 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14941 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    15001 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    15061 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    15121 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    15181 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    15241 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    15301 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15361 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15421 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15481 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15541 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15601 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15661 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15721 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15781 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15841 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAJTXY010000091        15819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTXY010000091 DAJTXY010000000
VERSION     DAJTXY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.091"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..882)
                     /locus_tag="OO566_002929"
     CDS             complement(<1..882)
                     /locus_tag="OO566_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6606145.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGA"
     gene            989..1285
                     /locus_tag="OO566_002930"
     CDS             989..1285
                     /locus_tag="OO566_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6606146.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="OO566_002931"
     CDS             complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="OO566_002931"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6606147.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="OO566_002932"
     CDS             complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="OO566_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6606148.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="OO566_002933"
     CDS             complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="OO566_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6606149.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4298..4381
                     /locus_tag="OO566_002934"
     CDS             4298..4381
                     /locus_tag="OO566_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6606150.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4435..5787)
                     /gene="gorA"
                     /locus_tag="OO566_002935"
     CDS             complement(4435..5787)
                     /gene="gorA"
                     /locus_tag="OO566_002935"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6606151.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5859..6701)
                     /gene="rlmJ"
                     /locus_tag="OO566_002936"
     CDS             complement(5859..6701)
                     /gene="rlmJ"
                     /locus_tag="OO566_002936"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6606152.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6904..8946
                     /gene="prlC"
                     /locus_tag="OO566_002937"
     CDS             6904..8946
                     /gene="prlC"
                     /locus_tag="OO566_002937"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6606153.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRSGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8954..9706
                     /gene="rsmJ"
                     /locus_tag="OO566_002938"
     CDS             8954..9706
                     /gene="rsmJ"
                     /locus_tag="OO566_002938"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6606154.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9755..11224)
                     /gene="dtpB"
                     /locus_tag="OO566_002939"
                     /pseudo
     CDS             complement(9755..11224)
                     /gene="dtpB"
                     /locus_tag="OO566_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11530..11964)
                     /gene="uspA"
                     /locus_tag="OO566_002940"
     CDS             complement(11530..11964)
                     /gene="uspA"
                     /locus_tag="OO566_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6606155.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12355..12690
                     /gene="uspB"
                     /locus_tag="OO566_002941"
     CDS             12355..12690
                     /gene="uspB"
                     /locus_tag="OO566_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6606156.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12761..14260)
                     /gene="pitA"
                     /locus_tag="OO566_002942"
     CDS             complement(12761..14260)
                     /gene="pitA"
                     /locus_tag="OO566_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6606157.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14492..15691
                     /locus_tag="OO566_002943"
     CDS             14492..15691
                     /locus_tag="OO566_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6606158.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPVLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITT
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3980 a         4048 c         4152 g         3639 t
ORIGIN      
        1 tgcgccggca ctgccgatga ttaatgccat taatgctccc agacccattc cttttgtcat
       61 cagaacagat gccagaggga taaccgcctc agcccggata tacagcggaa taccgacaac
      121 ggcgcttaat gggatggcga agggattatc tgctcctgca tgagcggcaa tccacgctga
      181 gggaataaag ccataaataa aagatcctat taatactcct aacaaaagat atggcagtac
      241 atctttaaac tgttgcaacg catctttggt ggctagtttt atgggattaa tacttactac
      301 agctttggta ctgcaacagg taactacggg gttctcaatg gctttagccg ctggactaca
      361 acaactcact tttatgggcg tatatgtcgt tcccggcgaa gtttttgctg gagcacaaca
      421 atttgctgat gagcttttac tggcaatgat atgacgttca aaccccagag aatccaggat
      481 aatactggca agaacggaga cgccggctgc gataatcgcg tacaacaagg tgactttcca
      541 accaaaggtc atccacatta gtccgacgat aatgggatta agtaatggtg aaacaaataa
      601 aaaagtgaga gtcggaccaa acccggcttt cgctgacaac aatccacgta acatgggtat
      661 tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga gcagccagga gatagcctct
      721 cccttttctg gcccccatca tctgctggat tttatggtct ggcacctttt gtcttatcag
      781 gctgacaccg gcactaatca ctataaacag caacgaaagc tcgacggcga gaaacacaaa
      841 catctctgcg gcatcttgca gcatcgcaag ccaactactc atacgtaacc tcattgggta
      901 aatctatatt tccagaataa tcgaaatgaa attcagatca agatatttct ggtattatcg
      961 aaatatcaac cagacagagg ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa
     1021 agagctaggt cacccgaccc gtttgttcat atttaaacat cttgtcaaag ccggggaaca
     1081 agggttgccg gtcggtgagt tgcaaaagca gcttgggata cctggctcca cactcagcca
     1141 ccatatatca gcactggtat cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct
     1201 gatgtgtgtt tcgcaatacg caatgctgga agcgataatt gaattcttac gcgaggagtg
     1261 ctgcgtaaat agcaaaattg cctgatgcgc tacgctctac atatttcatg ccatatattg
     1321 aatttgcacg gttttgtagg tcggataaga cgcgttagcg tcgcatccga cataaacaaa
     1381 gcgcacttta ccaactatcg gcagtggata tcgccgcttt atttcaggcg cttacccgct
     1441 tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc atctggcaga
     1501 atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt caccacaatc
     1561 gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat
     1621 ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt
     1681 acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc
     1741 agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc
     1801 gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac
     1861 aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac aggcagggtc
     1921 atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt
     1981 acgtgcagcc agagcagcgt tgccaggcta ccaatcgggg taattttcgg ccccaaatcg
     2041 cagccaatca cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca
     2101 tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag
     2161 gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg
     2221 agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac
     2281 atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt acccgtatta
     2341 atcgcatgac cgcgtttagc gacgacaaat aatatcagtg cgcccacagc tgcaatggcg
     2401 ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca
     2461 acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc
     2521 gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg
     2581 gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg
     2641 ccaaagaaat cagcggaaac gatattcacc aagttggaga caataagcgg caggctggcg
     2701 gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct
     2761 aaagccagca gcatggcgat gacaatcggc gtcaaaataa gcgccgcgcc atcattggca
     2821 aacagggccg caacggcggc accgagcagg acaatccagg taaacagcaa gcgaccgcgt
     2881 ccattgcccc agcgtgaaac gtgcagcgct gcccattcaa aaaagccgga ctcatccagc
     2941 agcaggctga tgataatgac ggcaataaac gccgccgtcg cgttccagac gatattccac
     3001 accaccggaa tatcacccgg atgaaccacg cccgtaacca acgccagtac cgcgccgagc
     3061 gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac gatggtcagg
     3121 acaaagatag cgcctgccag taacataatg cctcccggat aaaacacatg tgcaaatcca
     3181 tatgtgttta gctaaatttt taactgcaaa tgttcttact gtctccggaa cagttttgtc
     3241 tggccaggtt gcggacaatt gcctgaatct tttcctgttc acatcgccag gcttcatcaa
     3301 taattttcgc cgcccatgct ggaatatgcg gcgataagcg gtaatgaacc cacttacctt
     3361 gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc cagatggcgg gatatcttgg
     3421 gctgcgactg atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa
     3481 gcagtaaaac gatgcccaga cgggtttcat cagcaagaat tttgaacaat tggatgggta
     3541 acagaaatga catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata
     3601 agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa
     3661 ttttgagttg gctattcatt tgaaaggcgg tttgaatcag gacgtaaaaa aaaccgactt
     3721 tgcgtcggtt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
     3781 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
     3841 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
     3901 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
     3961 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
     4021 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
     4081 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
     4141 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
     4201 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
     4261 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
     4321 tcttggggcg ttaattgtgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
     4381 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
     4441 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
     4501 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     4561 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     4621 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     4681 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     4741 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     4801 cttgttatta aacaagcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     4861 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     4921 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     4981 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     5041 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     5101 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     5161 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     5221 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     5281 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     5341 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     5401 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     5461 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     5521 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     5581 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     5641 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     5701 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     5761 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     5821 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     5881 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     5941 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     6001 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     6061 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     6121 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     6181 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     6241 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     6301 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     6361 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     6421 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     6481 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     6541 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     6601 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     6661 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     6721 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     6781 aggccgccgc cgcgaaagcg taacgtttcc cattgaaatt cactacactt aaccccatgc
     6841 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     6901 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     6961 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     7021 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     7081 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     7141 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     7201 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     7261 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     7321 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     7381 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     7441 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     7501 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     7561 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     7621 agcacccgcg cctccgatca aggcccgaac gccggtaagt gggataacag caaggtgatg
     7681 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     7741 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     7801 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     7861 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     7921 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     7981 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     8041 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     8101 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcagc
     8161 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     8221 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     8281 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     8341 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     8401 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     8461 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     8521 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     8581 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     8641 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     8701 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     8761 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     8821 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     8881 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     8941 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     9001 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     9061 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     9121 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     9181 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     9241 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     9301 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     9361 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     9421 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     9481 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     9541 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     9601 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     9661 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     9721 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     9781 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     9841 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     9901 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     9961 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
    10021 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
    10081 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagtcg
    10141 tcaaaaagcc cagtgagcac ataaacatgc cgagagtaaa tttcatcggc atcgagaggt
    10201 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
    10261 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
    10321 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
    10381 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
    10441 tatccagctt gaatgcctga cgaaagaaga tgatggtgac gacgatggag agaacaatca
    10501 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
    10561 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
    10621 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
    10681 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
    10741 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
    10801 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
    10861 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
    10921 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
    10981 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
    11041 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
    11101 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
    11161 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
    11221 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
    11281 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
    11341 tattacaaca gaaaataacc agctgatcac actaatgatg gtgttaaatt ttgtattggc
    11401 aggagatttt gattgcgtac gcagatcgta ggccagataa ggggtttacg ctgcatcagg
    11461 taacaaggcg cgccaaaaag caaaacgccc gcatatgcgg gcgttcagga acacgtcgtg
    11521 gagggatgat tattcttctt cgtcgcgcag cggaacaatc agcatatcaa cgtgaacggt
    11581 gttgatcagc tgacgtgcgg aagacatcag tttgctccag aagtcctggt ggtgaccaca
    11641 aaccaccaga tccatatcgt atttcttgat tgcatcgacc agaacctggc ccaggtcgcc
    11701 gctgccgctc agggtttcag tgattggata gcctgcatta gtggaaagct cggtcagcgc
    11761 atgatgtgtc tcttcagaga tgcgtttctg catatcaccc agattcacat caataagccc
    11821 ggtgtatagg tcagagtagt ttacatctac gtggatcaga gaaactttcg cattgtaggg
    11881 gcgagccata gagactgctt tctctaccag aactttgctt tccggggaga gatcgactgc
    11941 gatgagaatg tgtttataag ccatagtgtt actccttcca taaagttgtc gatgactggc
    12001 cagctagcgt ttcttgtgcc gttacgtcac ccgcgtcctg cgaactacgt gcgcggggct
    12061 aacccagccg taaagattca gtgttaagac catccttacc ttatagcgac ccggatgatc
    12121 cgtcaatccg ccttgcttac ccgttagata aacaaatttt cgacatgaat atattgatag
    12181 tggttaacct tctggaaaaa aaacaacctg atctcctaca ctatctatag agccgctcgg
    12241 atgttggtca ccacaaaagc ggtttctcca ggacctggtg ttaactgatt ggcagcgtat
    12301 cgggagcggt agccccgggg tggaactccg cgggcaggtc gccggggagg aggtatgata
    12361 agcaccgtcg cattattttg ggctttatgt gtcgtttgca ttgttaacat ggcgcgctat
    12421 ttctcatcac tacgcgcgtt gttagtggta ctgcgtaact gcgatccatt gctctatcaa
    12481 tatgttgatg gagggggctt ttttacctca catggccaac ccaacaaaca ggtgcgtctc
    12541 gtttggtata tctatgccca acgttatcgc gatcatcacg atgatgagtt tattcgccgc
    12601 tgtgagcggg tgcgtcggca gtttattctg actagcgcat tgtgtggtct ggtggtggtc
    12661 agcctgattg cattgatgat ttggcattaa gcacttcagc aataataacg cataaaaaaa
    12721 gcgggccagt aaactgaccc gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga
    12781 accagtacag cccgccggaa agtagtaccg cagccggaag ggtaaacacc caagccatca
    12841 gaatactggt tacggtttta cgctgtaagc cgccgccatc taccaccatc gtccccgcga
    12901 cagaagagga gagtacgtga gtggtggaaa ccggcatccc ggtataactc gccaggccga
    12961 ttgacactgc cgccgtcatc tgggcagaca tcccctgagc gtaggtcatg cctttcttac
    13021 cgattttctc accgatagtc gttgccacac gacgccagcc aatcatcgta ccgataccta
    13081 acgccagcgc gaccgccatg atgatccaca ccggtgcata ctcgatggtg ctaagcatgt
    13141 cggacttcag tttcttcaac aggcgctgat cgtcagcact cacaccaggc atcttcacca
    13201 ctttgtcgat agtgtcagaa acgcacagca taatgcggcg catctggcta cgttgatcaa
    13261 gcgacagctt gtcgtagctt tccacatcgg tggtcaacat acctttcagg cggttgagcg
    13321 cgttaatggt attcgacgga tggcagtgga actccgcagg ttgcgttgcg ccagcttccg
    13381 gagccggtac taactgatca gcaccggtag cctgtttgag cagcgcagga tgctgctcaa
    13441 agtaagcttc gacgttgttg atggcatcac gggtacgggt gatttcgtag ccagtggcat
    13501 tcatgttcac cacgaagcct gctggcgcga cgccaatcaa taccaacata accagaccaa
    13561 tgcctttctg accatcgttc gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca
    13621 gcgcgatacg cgtccagaac ggcggctttt tcttgccgtc tttcttttca cgctccgctg
    13681 gggtcaggtg gatacgggcg cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa
    13741 tcagaccgcc agcaaacacc aggccgacaa taggggaaac gatcagagaa ccgaaaatac
    13801 ttaatacttt cgggatattg agtgcatcca ccactgacgt cccggtcatc aacgcattgg
    13861 ttaaaccaat cccgatgatc gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa
    13921 agtaccaggt acccaggttc cagataatcg ccgccagcaa catagagaac accatggcaa
    13981 ggccatgaga cgatcccata ttaagcagca gatccgtcgg cagcatatgc acaatggcat
    14041 aggcaacact cagaccaccc agcaaaacac ccaaaaagtt gaacaccgcc gccataacca
    14101 cggcgagctg agaacgcatc gcgcgggtat agataacggt tgccacggcg ttggctgtgt
    14161 catggaaacc attgatggct tcgtagaaca gcacaaaagc cagtgcaagc aataataaca
    14221 gcccggtatg caaatccagg ccagcaaaca aatgtagcat aggacgttac gccattttga
    14281 ggacatgaac gcggcgcatt atcagtgact ttcgcggcgc gggcaaagtg aaatatagac
    14341 tttttttgat ttgcctcctg tatggatttc actcaaaaaa taattatctt atataattca
    14401 ggcaaatact tccttttagt aatattgatg ctggtgcgac cactgaggaa tctttacaat
    14461 tcacgcccgt tttttctaag aggagcgcaa cgtggaaagg tttgatgcca ttattatagg
    14521 cgctggtgcg gcgggtatgt tctgttctgc gctggcaggt caggcaggac gccgggttct
    14581 gctgatcgat aatggtaaaa tactagggcg caaaatcctt atgtctggcg gtgggcgctg
    14641 caactttacc aacctttatg tcgaaccagg cgcttatctg agccagaatc cgcatttttg
    14701 taagtctgca ctcgcacgtt ttacccagtg ggatttcatt gatctggtca ataaacacgg
    14761 tatcgcctgg cacgagaaaa cgttagggca actcttctgc gatgactccg cgcagcagat
    14821 tgtcgacatg ctggtggatg agtgcgagaa gggcaatgta accttcagat tgcgtagcga
    14881 agtgctgagt gtggcgaagg atgaaacagg cttcacgctt gatctgaacg gcatgactgt
    14941 cggttgcgaa aagctggtca tcgcgaccgg tgggctgtca atgccggtgc tgggcgcatc
    15001 gccgtttggt tataagattg ccgaacaatt tggcctcaac gtgctgccga cccgcgcggg
    15061 tctggtgcca ttcactctgc ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt
    15121 ggcggtgcct tccgtgatta ccactgaaaa cggcaccgtt ttccgtgaga acttactctt
    15181 cacccaccgc ggcttatctg gaccggcggt gttgcagatt tcaagctact ggcaaccggg
    15241 tgaatttgtc agtatcaatc tgctaccgga tgtggacctc gaaaccttcc tgaatgagca
    15301 gcgtaacgca catccgaatc aaagcctgaa aaacacactg gcggttcatc taccgaagcg
    15361 gttggttgaa cgcttacagc aactcgggca aatcccggat gtttcgctaa aacagctcaa
    15421 cgtgtgtgac caacaggcac tgattagcac attgaccgac tggcgcgtac aacccaacgg
    15481 cactgaaggc tatcgcactg ccgaagtgac gctcggcggc gtggacacca acgaactttc
    15541 ttcacggacg atggaagcgc gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga
    15601 cgttaccggc tggctggggg gctataactt ccagtgggcg tggtcgagtg catgggcttg
    15661 tgcgcaggat ttggcgtcaa ttgtgaaata accatgacgt tttcccgtca gccgtttgta
    15721 tatgtcgtta ttaagtcatt ttggtacggt tctgcatccg acattctggt gttaactcgc
    15781 tgttttgtta gcgaattaac gctattgccg ttgtggtta
//
LOCUS       DAJTXY010000092        15673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTXY010000092 DAJTXY010000000
VERSION     DAJTXY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.092"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..396)
                     /locus_tag="OO566_002944"
     CDS             complement(<1..396)
                     /locus_tag="OO566_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6606159.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTED"
     gene            complement(393..3077)
                     /locus_tag="OO566_002945"
     CDS             complement(393..3077)
                     /locus_tag="OO566_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6606160.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            complement(3169..3669)
                     /locus_tag="OO566_002946"
     CDS             complement(3169..3669)
                     /locus_tag="OO566_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6606161.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(3699..4415)
                     /locus_tag="OO566_002947"
     CDS             complement(3699..4415)
                     /locus_tag="OO566_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6606162.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            4711..5487
                     /gene="exuR"
                     /locus_tag="OO566_002948"
     CDS             4711..5487
                     /gene="exuR"
                     /locus_tag="OO566_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6606163.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            5832..6494
                     /locus_tag="OO566_002949"
     CDS             5832..6494
                     /locus_tag="OO566_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6606164.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            6498..6881
                     /gene="mzrA"
                     /locus_tag="OO566_002950"
     CDS             6498..6881
                     /gene="mzrA"
                     /locus_tag="OO566_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6606165.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            7028..7382
                     /locus_tag="OO566_002951"
                     /pseudo
     CDS             7028..7382
                     /locus_tag="OO566_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            7420..7725
                     /locus_tag="OO566_002952"
     CDS             7420..7725
                     /locus_tag="OO566_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6606166.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            7728..8132
                     /locus_tag="OO566_002953"
     CDS             7728..8132
                     /locus_tag="OO566_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6606167.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            8122..8421
                     /locus_tag="OO566_002954"
     CDS             8122..8421
                     /locus_tag="OO566_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6606168.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            8607..8999
                     /locus_tag="OO566_002955"
     CDS             8607..8999
                     /locus_tag="OO566_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6606169.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTTLFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            9069..10055
                     /gene="yqjG"
                     /locus_tag="OO566_002956"
     CDS             9069..10055
                     /gene="yqjG"
                     /locus_tag="OO566_002956"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6606170.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            10348..10716
                     /locus_tag="OO566_002957"
     CDS             10348..10716
                     /locus_tag="OO566_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6606171.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(10923..11819)
                     /gene="yhaJ"
                     /locus_tag="OO566_002958"
     CDS             complement(10923..11819)
                     /gene="yhaJ"
                     /locus_tag="OO566_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6606172.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            11924..12625
                     /locus_tag="OO566_002959"
     CDS             11924..12625
                     /locus_tag="OO566_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6606173.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12648..12812
                     /locus_tag="OO566_002960"
     CDS             12648..12812
                     /locus_tag="OO566_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606174.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(13024..14316)
                     /locus_tag="OO566_002961"
     CDS             complement(13024..14316)
                     /locus_tag="OO566_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6606175.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14344..>15673)
                     /locus_tag="OO566_002962"
     CDS             complement(14344..>15673)
                     /locus_tag="OO566_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6606176.1"
                     /translation="EIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTCWVIMSI
                     GMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSVI
                     SGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGLV
                     LICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLVK
                     NAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFFY
                     AVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFGV
                     YLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTSI
                     CSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT         4063 a         4005 c         3636 g         3969 t
ORIGIN      
        1 atcttctgta catgaagatc tattagaatc cttaagattc gtctgaagcc ctttgccccc
       61 cttgcatcct agcggcgctt catcgctact tacaatggct gttttaaatc ctttgagttt
      121 taccggccaa cgcatcccac tgcgttgttc agttaaatag atttgtactg tcgatatatt
      181 tccattagct gtacaggttt gttggaggtc aggatacgaa cattcatacc acatatatcc
      241 attatttcct gccgacgctt catccttcca gaagtaataa tccgctctac cagatgccaa
      301 atcatacgta atttcactgg tcacgtcagg agctgcctga acgccagtga gcgtgccaaa
      361 caagcccaat atcgccgcaa tcaatcgatt tctcatggtg caacctccct ttgcgccgtc
      421 tgaacgtttc cacgtaattt aggctgtttc aactgtgcct gaacctgaat cgactccggt
      481 aattgtgaat aagtcaccac actacaagta acatctccgc tgtaacgaat gctggcgcgt
      541 ttctttacaa cactcagtgg acacttaagg aattgctgcc cggaaagcag atagagctct
      601 ttttcgctgc cgttaatttc aagtgtaaat ccgccatctt cagctaccag taacatggcg
      661 ctgttcagcc ccacaatcgg gaattttttc accgctgccg gcagtaataa acgaccgata
      721 tagttgtagt taaagctggc agacacttcg cgatagcgca gcttgccagg caacaacatt
      781 aattcacgat tccctgcacc gttaatgatg tttgttgtcc cctgctgatt gccctggcta
      841 ctgtcgttaa cctcaacaaa accagactga aatccaggat aagggaacaa cacactgtca
      901 ctttgaccaa gctgcaaagt tcggctgcct gccacccgcg cttcgagttg cagatcctga
      961 ctctcttcat cctgttcaga gaagcctttc acatccacca gtaccgcacc cagcaaatcg
     1021 tctgtaccac tggctcccaa attaacgcca tagcgactga cagccagcgt agagctataa
     1081 gtgcccgtaa aggccgaaag atgatcatga ttcttacggt cataactatc agatacggta
     1141 gcgtagacat ttccgtattg cccatcccaa cgcccgttaa ccgcgctgtt tatggtatcg
     1201 gtgttcaggc ctccgacggt tacgccaagt tctttatggc tgaatgaatc gttataaaag
     1261 gtatgcgata actgccatga cgtttgatcg ccatcctgtt cgctataacg ataatccgta
     1321 gacacattgg ttgaatggct gttgttattg ctatccatcg ttggcgtgtc agataacgaa
     1381 aacgataagt acaagccgtt gtcgtcgtta tcgccaccgt tgtcattacg atggaaaaca
     1441 ccgactgacg tactcaaaat ccagtcacgc atcacaatag cgcgggaagc gctaagttgt
     1501 acagtttcag agatactttg cgctgttgtc tggcgaagat agttattttc aaagctctgc
     1561 tgtttatccc aacgcaaact atcatcagtg ttattttgtg tgcaggtata acccagcatc
     1621 atattccagc cgacaaaatt cagtgaaact gtcgcggaaa tactttcata acagcttaag
     1681 gcgttatagc tctctctgct gtcggttgta caagcatcgc cgtcagagtt ggtccgataa
     1741 aagcccaacg tcggccatcc cggattactc cagttggcct gttgcatatc acctttgccg
     1801 ccgtcatcgt tacggaacac gctggcgctg attgcaagat tccccacccc gcctaaatct
     1861 gccgtccagt tattacctaa ctcgaaagca ctcacatcat cggcattcgc cagccctgcg
     1921 tagagctcat attgcggatg taatggcagg cgtactccta gctgataagc tgaactttca
     1981 tcatcagaaa cctgtgatgt agttttacct gcctgtaaga accattgcgc atttccgtca
     2041 gtcagaccgc cggttttggt aaacggcaca agctcggtgc gggtgagttg gttattttcg
     2101 tagactttta atgccacgct gtagctgcct ggcggaaagg aactggtatc aataaattgc
     2161 gaaccactat tgagataaaa cgatcccaaa agttgctcat tacgataagc gtcaacacga
     2221 gaattgcgcg aaagcagaac cattactggg gttccttgct ggctttgcgc ctggtttaaa
     2281 tagctcagtg tcgacccgat acgcatccca tcgattgcac cgagtggcag aaagttaaag
     2341 gtaaagttcc cgccttgtgc attaaatagt gtgcggttgt ccatgcgacc cgcctgcaca
     2401 taataacgac gtaaaaaatc ataacgataa tagagatcgc tgacgtcagt attactgtca
     2461 ctgacatcat ctgcatcata accgttgaaa ttccagtgtg caccaatata gctattttct
     2521 gttattccca gcgcaccgtt tccctgaata gacaacgatt ggtaatcatc ctgtgccaga
     2581 acattgatgt cttgctgatg tataaaagcg ttaacagtat ttttgtctgg atttaaatat
     2641 aatgacttcg catcgaaagc tgaattccat tgcggattaa taaacaacgt tgccgaactt
     2701 tcttcatcat tatagattat ttccagcgta tcggtattca gaaaaccaca ccctgagtca
     2761 gtacgtacat aaccgcaagc caattcaccg tggcgagcta gcggctgcga taatttttct
     2821 ttcactatat gcgcgatttt ctgatcgtct acatccagtt ccagcttgtt atatagactt
     2881 gctggatcaa gaaatgttac ggtatcaaga ttaaccattg cataatattt accgagactt
     2941 ttccctgaaa aataaacctc gatatactcc tgctgtccct gagcaataac ctcaaaccct
     3001 gcgggaattt tggttaatgt cgcctctgcc gggctgagac tcgccaggat caaaagagcc
     3061 agtaattttt tatccatggc aatcctgaaa tccataacaa atactaaaac agttaaaata
     3121 agggagggct acccctccct ttgttacgtg ttaattctgt tatccggatt aaatatcctg
     3181 agacagataa atgcttacta caccacggta gatgcctgtt tccagaacac ctttggtggt
     3241 ttgagcaaac atcaggtttt tagccagaga accgttagtt aacgcatcgg aagcaaagat
     3301 tttggttgcg gtaaaagtgg ttgccgcatc agctttaatt tcttcaccac cccaggttac
     3361 tgtcagcgga acaatcttgc tagcatccag gctttgaacc agttgcgctg gggacactaa
     3421 ctgcattttt acatctttgg taacgtcgtt agaccagatt ttggtcatca actggtaaga
     3481 ttgcagaccc tgccccggca gatattgcat ttctactgct ttcggcaacg cggtgttatc
     3541 ggtctgtgtc atatccagag cggcatcaac gttggccgtt acagtaatat ccttttgtac
     3601 tgccaatgcg gagcctgcaa cagaaaacat cgctgcgacc agaagagatt ttgcaaatac
     3661 ctttttcatt actattcctt cgtttattac aatgataatt aatggaccgc cagttcaaac
     3721 tcgccattat ctttggtgat ccagttatta tatttgacaa cgattttgtc gtagccagat
     3781 atgctttcaa gatttctttc ctgaccaggg aatatgtttt tatttacggt ctgtatctta
     3841 cagttttctt tgctattatt tttacaataa gcgatcgtta aaagctttaa gcgttgattc
     3901 cctgcattaa ttaatttttg ggcagaattt acttctaact tagctatagg ttgctgaggt
     3961 ggaacactaa caacgattcc ccaaattaag ttaactgtta actgcataga gagatctttt
     4021 ttatctgtgg caacgtcatc aatactggga accgcttcaa atttaacgcg ataatttttc
     4081 tctttttgct ctggttccat cgcaacaaaa cgtacggttt tgctactacc ggctggtaaa
     4141 gcgaatttag gtggcataac aaccagatga tttgcatcac cggatttaat ctcaacttca
     4201 ttttcctgag gcgtcgatgg attatcgata cggaatactg tcgccttgat gtactgaact
     4261 tcattacttt tagatattac gcgaacgttg ccttcaccct gactattaat ggaaacaggc
     4321 ataggataaa cagtcatatt tgactgtgcc aaaaaaggag cgaacacgaa taaaagcggc
     4381 gtaagccgta ataagagatt aattttcgcc accatataat tttgatctta caatatcgtt
     4441 tcaaaaaagt tggtgtatta gagatgtaga tttattgtaa gtcaatataa aaaagcattt
     4501 ttagttaaca ttaaatttaa aggctgggaa ttaacttact gaaatttagt agctttcaca
     4561 atgacgatac ttatttttta ggaatttata aatattcaca atattaattt aatacattaa
     4621 ttcaatcatc aaatttatat atgaccaatg gcatacccat gaataaagtg gtatgataac
     4681 ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg ccgtttgtat
     4741 caacaacttg ctgctgacct gaaagagcgc attgaacagg gcgtctatct ggtgggtgat
     4801 aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac ggtagttcgt
     4861 gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg ttcaggtatt
     4921 catgtggttt ccaaccagcc gcgtcatcag caggcggctg acaataatat ggaattcgcc
     4981 aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa tattgccgag
     5041 ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat tcaggaacag
     5101 gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca cattcaggta
     5161 gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg gacccagcgt
     5221 agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac cgttgataac
     5281 tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc tcatgctgct
     5341 aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa cgaaaccagc
     5401 gacgatttcg agttcaatgc cgaccgctat ctgttcgccg aaaacccggt agtgcatctc
     5461 gatacggcca ccagcggcag caaatgaaat tcctgtccga caggcgctgc aatgcgcctg
     5521 tttgtctcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc tctcgccacc
     5581 ctcccctgca atcagcaaaa tcagactcca cggacatgta attttgataa cgaacaacgt
     5641 tgacctttgt tacaattaga ttcaatttga atttatgctt ttgaatgctt tcttatctca
     5701 cgttttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga acataccaaa
     5761 acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa ccgattcacc
     5821 aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg caggattttg
     5881 aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt gtaattttgt
     5941 tccttgaaaa tggcttgctt ccggcggcct ttttaccggg cgacagttta ctggtattgg
     6001 tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg ctgctgaccg
     6061 ttgccgccag cctcggttgc tgggtcagct atattcaggg gcgatggctg ggcaataccc
     6121 gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc gcacaccatc
     6181 tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgca tttgtcagaa
     6241 cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt cagttcttca
     6301 actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac atgctcggca
     6361 aaacgccggt atttttaaag tacgaggacc agctgatgtc atgcctgatg ctgctcccgg
     6421 tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa aagaaatatg
     6481 gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta gcctggtccg
     6541 gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt cgccagcaag
     6601 agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac ggtttttcga
     6661 tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc aaaaacgaca
     6721 ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg gtcctcgaca
     6781 gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag gctatgcagt
     6841 ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt atccgaaaca
     6901 tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg gtggttaaca
     6961 ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca caatggaagg
     7021 ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta gtgccggtag
     7081 ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga tcagctatgc
     7141 cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga gtgaagtccg
     7201 ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg caaagcagaa
     7261 agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag gcgatgccga
     7321 tagaacggaa actggcagaa gcgcaggaag agctgaaaaa gctggaagcg cgcgactact
     7381 aactcacaat agtcactact tactcacctg gagaaaacta tgtcgaaaga acacactacg
     7441 gaacatctgc gtgctgagtt gaaatccctt tccgatacgc tggaagaggt gcttagctca
     7501 tctggcgaga agtcgaaaga agagttgagt aagattcgta gcaaagcgga gcaggcactg
     7561 aaacagagcc gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca aacccgtgtc
     7621 gcggcggcgc gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt gggaattggc
     7681 gctgcaatcg gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg gcggacactc
     7741 atcacgcgca agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca
     7801 tggttgaaat ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag
     7861 ctaatctctt tcaactttta ctgatgctgg gcctgacgat gcttttcgct gcatttggtc
     7921 ttatgagcct gatggtgcta attatttggg cggttgatcc gcaatatcgc ctgaatgcga
     7981 tgattgccac caccgtggtg ttgctgctac tggcactgat tggcggtatc tggacgttac
     8041 gtaaatcgcg taaatccacc ttgctgcgcc atacacgcca tgagctggca aacgatcggc
     8101 aactgctcga ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg taaggcgcaa
     8161 ctgcttagcc agatccagca acaacggctg gatctttccg ccagtcgtcg tgaatggctg
     8221 gaggcaacag gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg ctcctgggcg
     8281 ctggttggca gtagcgtgat ggcgatctgg acgattcgcc atcctaatat gctggtccgc
     8341 tgggccagac gcggttttgg cgtatggagc gcctggcgtc tggttaaaac gaccctcaag
     8401 cagcaacagc ttcgcggtta acttccctct ggccggagca attccggcct tatccctcaa
     8461 attttttgaa tatttttgac agttttcctt gctaacaatc atcattcacc acgtttatga
     8521 ttctctccat cgacagcaac gacgctaata ccgcgccctt gcacaaaaaa acaatcagca
     8581 gcctgagtgg cgcagtggag agtatgatga aaaaattaga agatgttggt gtactggtag
     8641 cgcacatttt aatgccgatt ctgtttatta ccgcaggctg gggaaaaatc accgcttact
     8701 ctggtaccca acaatatatg gaagcaatgg gtgtcccggg ttttatgctg ccgctggtga
     8761 ttctgcttga gttcggtggt ggtctggcaa tcctattcgg tttcctgact cgcaccacaa
     8821 ccctgtttac tgcgggcttt acgctgctga cggcattttt atttcacagc aactttgctg
     8881 aaggcgtcaa ctcgctgatg ttcatgaaaa acctgacaat ttctggcgga ttcctgctgc
     8941 tggcaattac cggtccgggc gcgtatagca tcgaccgcct gctgaataaa aagtggtaag
     9001 cacgcgctat acttaacgat aaaaaacgag gaggaagctc ctcgtttttg ctattggagg
     9061 agagaaaaat gggtcaactg attgacggcg tctggcatga cacctggtac gataccaaat
     9121 ctaccggcgg taaatttcaa cgttcagctt ccgcatttcg taactggctc actgccgatg
     9181 gcgcacctgg ccccactggc acaggcggtt ttatcgcaga gaaagatcgt tatcatctct
     9241 atgtttcact cgcctgcccg tgggcgcacc gcacgctgat catgcgcaaa ctcaaaggac
     9301 tggaaccgtt tatttccgtt tccgtagtga acccgctgat gctggaaaac ggctggacct
     9361 ttgatgacag ttttccggga gcaaccggag atacgctcta tcaacatgaa tttctgtatc
     9421 agctttatct ccacgccgat ccacactaca gcggacgagt tactgttccc gtgctgtggg
     9481 acaaaaagaa ccacaccatc gtcagcaacg aatcagcaga aatcatccgc atgttcaata
     9541 ccgcgtttga tgcgctgggc gcaaaagcgg gtgattacta cccaccagcc ctgcaaacga
     9601 aaattgacga acttaacggc tggatttatg acaccgttaa caacggcgtg tataaagctg
     9661 gttttgccac cagccagcaa gcctacgacg aggcggtggc gaaagtgttt gaatcgctgg
     9721 cgcgactgga gcagatttta ggccagcatc gttacctgac cggcaaccag ctaaccgaag
     9781 ccgatattcg cctgtggact acgctggtgc gttttgatcc agtgtatgtg acccacttca
     9841 agtgtgataa gcaccgcatc agcgattacc tgaatctgta tggcttcctg cgcgatatct
     9901 accagatgcc ggggatcgcc gaaactgtca atttcgatca catccgtaat cattacttcc
     9961 gcagccataa gaccatcaac cctacgggga ttatttcaat tggtccgtgg caggatctcg
    10021 atgaaccgca tggacgagat gttcgcttcg gttaaaaaaa ggcaccttac gaggtgcctc
    10081 tgataattag ctaagtgcat gttgttcatg tcggatgcgg cgtgaacgcc ttatcaaacc
    10141 tacaaagcgt ttatattcaa tatgttacaa tgaccgtgta ggccagataa gcgtagcgca
    10201 tctggcgatt ttgcgcttgc catcagtctt agcgcgcctt cacggcactt tattaattcc
    10261 ttgaacgaat atttactgcc atttcattca cgtctattct taatttgctg ctttaaaaac
    10321 aagtgattga gcaaaattga ggcaaaaatg gactggtatc tgaaagtact aaaaaattat
    10381 gtcggtttcc ggggtcgtgc gcggcgcaaa gagtactgga tgtttattct ggtcaacatc
    10441 atctttacgt tcgtgctggg gctgctggat aaaatgttag gctggcaacg cgctggtggc
    10501 gaaggcatcc tgacgacaat ctacggtatt ctggtgtttt taccgtggtg ggcggttcag
    10561 ttccgccgcc tgcacgacac cgaccgctcg gcgtggtggg cactgctatt cttaatcccg
    10621 tttatcggct ggcttattat catcgtcttt aactgccagg cgggtacgcc aggcgaaaac
    10681 cgctttggac cagatccaaa actggaacaa gaataatgat taagcacctt gtaaaaggtg
    10741 cttcaaattg ttgacaaagt gcgctttgtt tatgccggat gcggcgtaaa cgccttatcc
    10801 agcctacaaa ttgccaaaat tcaatatatt gcagtgactg cgtaggcctg ataagcgtag
    10861 cgcatcaggc agttttgcgt ttgtcagcag tcagaagcac cttgtgaaag gtgcttaata
    10921 gattattttc cagcgaacaa ctttggaatt tcccgcagac accaggattt cgcttccccc
    10981 atactgtcac gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg
    11041 acacgcaacc gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg
    11101 ccaagccccg ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc
    11161 tgtttgtcca gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca
    11221 attccacgat atttcacacg cgtcacttca gataacggtt ccggctcctg atgaatcggg
    11281 tgatcaggcg cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc
    11341 gacgaggaac gaaaatgcat atccggcgcg ataacgatat ccgcccgccc ctgctccagc
    11401 cgttcccacg cccccgccag cacttctgtg atgattgcca gttgggtatt ggcttttgcc
    11461 gccagtttgt cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg
    11521 gtgagatgcg tttcccaacc gcgcgcgaga gcttccgcat cagtagtcag tttatctgcg
    11581 gcttccagca aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg
    11641 cgatggcccg agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatgatg
    11701 tagctaagtg cggaaggcac gcgtcccagc tcatccgctg ccgccgcaaa actgccccgg
    11761 cgatcgatcg catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt
    11821 tcgttctcat tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt
    11881 ccagcccctt accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac
    11941 tgccaggcag tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt
    12001 tggacactac ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc ttaaccagga
    12061 agtgctggcc ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa
    12121 tgtgattctg gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag
    12181 cgccggagag gcgttgctgc tctctaccca gccgggtgtc agctatagcg aacacaatct
    12241 cagcaaagac aaaccgttaa cgcgaatgca gctttggctg gatacctgcc cgcagcgaga
    12301 gaatccgctg attcaaaagc tggcgcttaa tatggacaag cagcaattaa tcgcctcgcc
    12361 agagggtgcg atgggaagcc tgcaattacg ccaacaagtg tggctgcacc atatcgtgct
    12421 cgacaaaggc gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat
    12481 tcacgggaaa tttcatgcgc ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga
    12541 tggggcgttt attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc
    12601 tttgctgata gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat
    12661 cgaccaaaaa gcgccagcta gcgaagcccg tggtggcgaa agaacctgct cgcaccgcca
    12721 aaaattttgg ctatgaagag atgttgagcg agctggaagc tatcgtcgcg gatgctgaaa
    12781 cgcgtttagc cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc ggcgtaaacg
    12841 ccttatccgg cctacaaaac acaacaacat caacaaatta aacaccccgt aggccggata
    12901 agatgcgcca gcatcgcatc cggcatcagg tacaaaacac aacaacatca acaagttgag
    12961 caccccgtag gccggataag gtgcgccagc atcgcatccg gcacaatact caaaacctgg
    13021 cgtttatctg gccttgctcg ccataatctc gataatctgc cgatccgttt gctgcatcga
    13081 atggcttgct aacgcacaca ggttggcaat cgactgctca acatcatgcg ccacgatccc
    13141 ttcattgccg gtcacggcgg tatcatccag cgccattaac accgctttcc acgcagccga
    13201 agcactggtc gaaaccttcg caccatcgca aatcatgccg ctgacatcgc cgatcatact
    13261 gctgatcgcc atcgagatag tttcataacg cccatccacc agccatgcca tcccggcggc
    13321 ggcccccatt gctgcggtcg ttgcggcaca aagtgcagac aaacgcggta actggttatg
    13381 gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagtcgtt catcatccgc
    13441 tccgaagtgt tctgctacaa ccaccacagg cattgttgcg gtgatcccct ggttacccga
    13501 gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct
    13561 ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct cgcactgttt
    13621 ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct gcgataacgc
    13681 acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaatggga cttcattgac
    13741 gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggcg actcttgctc
    13801 gccctctgcc acacacgcct gctgggtaaa caccacacca ttgtgcgtct caatatgcac
    13861 aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt tccagacttt
    13921 ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacagaga ctttccccgc
    13981 cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gcgtctttca gcacttccag
    14041 cccggcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca gccccaccat
    14101 tcccgtgccg ggaacggtga tgcccagacc gttcttcatc agatttggcg aaacccaggc
    14161 ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg ccagcgccag
    14221 tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta
    14281 acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa aatttcaggt
    14341 aaatcaagaa aatgccagga acagagaaac acaaagcaac aaaccagtga cgataatcag
    14401 gtacagagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca gccacgccgg
    14461 gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg
    14521 tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga tgccgcgctg
    14581 aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt tcattacgat
    14641 cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca tgacagcaaa
    14701 gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc taataaactg
    14761 tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc
    14821 catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc
    14881 gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt cccgcgagcg
    14941 ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca gggtaaacgg
    15001 cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac cgacgttata
    15061 cagatgccac attcccacca tcgacacgcc cagcgcagcg actaccagca gcttggtcag
    15121 caccatgtcg gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga tcgccaccag
    15181 aatgcaaatc agtaccagac cataaaaggg actgtctgac agcaaccctt ccgtcacgcc
    15241 gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga cgaacatcca
    15301 aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt tacctaaata
    15361 accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg tattaataaa
    15421 caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca aaaataccca
    15481 caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc
    15541 gatactcata atcacccagc aggtgtcagt actgtcgaat ttgatcgctt ctcgccactc
    15601 gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt
    15661 cgatgcaatt tcc
//
LOCUS       DAJTXY010000093        15488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTXY010000093 DAJTXY010000000
VERSION     DAJTXY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.093"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(70..1263)
                     /locus_tag="OO566_002963"
     CDS             complement(70..1263)
                     /locus_tag="OO566_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6606177.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     FEVTH"
     gene            complement(1462..1546)
                     /locus_tag="OO566_002964"
     tRNA            complement(1462..1546)
                     /locus_tag="OO566_002964"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1510..1512),aa:Leu,seq:caa)
     gene            1742..2761
                     /gene="ahr"
                     /locus_tag="OO566_002965"
     CDS             1742..2761
                     /gene="ahr"
                     /locus_tag="OO566_002965"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6606178.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2890..4392
                     /locus_tag="OO566_002966"
     CDS             2890..4392
                     /locus_tag="OO566_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6606179.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4511..5593)
                     /gene="lptG"
                     /locus_tag="OO566_002967"
     CDS             complement(4511..5593)
                     /gene="lptG"
                     /locus_tag="OO566_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6606180.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5593..6693)
                     /gene="lptF"
                     /locus_tag="OO566_002968"
     CDS             complement(5593..6693)
                     /gene="lptF"
                     /locus_tag="OO566_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6606181.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPIRRLRASFSRKGAV"
     gene            6960..8471
                     /gene="pepA"
                     /locus_tag="OO566_002969"
     CDS             6960..8471
                     /gene="pepA"
                     /locus_tag="OO566_002969"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6606182.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8605..9048
                     /gene="holC"
                     /locus_tag="OO566_002970"
     CDS             8605..9048
                     /gene="holC"
                     /locus_tag="OO566_002970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6606183.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9048..11903
                     /gene="valS"
                     /locus_tag="OO566_002971"
     CDS             9048..11903
                     /gene="valS"
                     /locus_tag="OO566_002971"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6606184.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11957..13153)
                     /locus_tag="OO566_002972"
     CDS             complement(11957..13153)
                     /locus_tag="OO566_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6606185.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSAGMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNSVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILTFLDLLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13346..13849
                     /locus_tag="OO566_002973"
     CDS             13346..13849
                     /locus_tag="OO566_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6606186.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13895..14311)
                     /gene="rraB"
                     /locus_tag="OO566_002974"
     CDS             complement(13895..14311)
                     /gene="rraB"
                     /locus_tag="OO566_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6606187.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14473..15486
                     /gene="argF"
                     /locus_tag="OO566_002975"
     CDS             14473..15486
                     /gene="argF"
                     /locus_tag="OO566_002975"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6606188.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3988 a         3950 c         3989 g         3561 t
ORIGIN      
        1 ggagtgtccg ttaaactcag gctacctcat tctgaggtga gcatatcacc tctgttcagg
       61 tggccaaatt cagtgtgtca cttcaaacat caacctacgc tccccaagat gttccgcctt
      121 atggatgtaa gctgctcgca cagagctacg ctcctggtga ctcatctgcc gctctaccgc
      181 atccctcgac cataatcccg actcaatcaa cgaactacaa gccatcgtcc tgaagccatg
      241 cccacaaacc tcgaccttcg tgtcataccc cattacgcgc aaagccttgt tcaccgtatt
      301 ctcactcatc ggcttacgtg gatcgtgatc gcccacaaaa atcagctctc gatttccgct
      361 catgcttttg atcttttcca aaatacttag ggcctgccgc gacaaaggta caaggtgtgg
      421 tgtccgcatc ttcgagccac gctgagaatg cttgactcct tccagttgct cacgctcacc
      481 tgggattgtc cacatagccg tttcaaaatc tacttctgac cagcgggcaa aacgcagctc
      541 gctcgaacgg atgaaaacca ataaagtgag ttcaaccgcc agtcgagtta acggtctgcc
      601 agaatagtga tctatgcgat gaagtaattc aggtatacgg ttcagttcaa gagctgcacg
      661 gtgctgtctt ttcgccgtag caaccgcacc tgcaatctct tgcgcggggt tgtagtcgat
      721 taagccgctc tgaacggcaa agcgcataat cgcggtagta cgctgctgta aacgggcggc
      781 aacttcgagc cgcccggatg actcgactgc tttaatgggt acaagaagat cccgcgtctt
      841 cagttccgca atgttccgct taccgatagc agtaaagaga ttatcctcca gacttttcaa
      901 aacacgagcg ctatgcgatg ccgaccactt ctgattgctg gcgtgccagt ctctggcgac
      961 tacttcaaac gttatcgcct cttgttcctg ttctacctta acagctttct tgttttcact
     1021 gggatcgaca ccattagcta aaagcttacg agcctcatcc cggcgtgccc tggcatccgc
     1081 caacgacact tcggggtatt tccccaatgc aagcatcttc tctttgccgc caaagcgata
     1141 acgtagccgc cagtatttgg agccgttagg gtgaaccagc aaaaccatgc cttcaccgtc
     1201 ggtcagctta taggcttttg cttcaggctt agccgaacga accttcacat cactcagagc
     1261 catgatgagt atcctttcaa gggttctgtg tgggtacaaa cattatcgaa ccgggaaata
     1321 cccgcagttg tacccgcatc agtaagttga tgtagattga atcaggttga cttaggttga
     1381 gtgaaaaagc gaggaaagcc ttgcggatac tggattttag gcacaaaaaa agacgtccgt
     1441 tgacgtctat tgatgttccg atggtgccga aggccggact cgaaccggca cgtatttcta
     1501 cggttgattt tgaatcaact gcgtctaccg atttcgccac ttcggcactg aagaggtatg
     1561 cggaaaacgt tgtggattat acctgtcgca cgccaccatg caagcgccag cttgcaccca
     1621 cctcgctaag tgctgaaatt ttcagcatta cactcagcga tatgtaacct ttgtcacact
     1681 ccaggcaccc cgccctgcca tgctctacac ttcccaaaca acaccagaga aggacccaaa
     1741 aatgtcgatg ataaaaagct atgccgcaaa agaagcgggc ggcgaactgg aagtttatga
     1801 gtacgatccc ggtgagctga agccacaaga tgttgaagtg caggtggatt actgcgggat
     1861 ttgtcattcc gatctgtcga tgatcgataa cgaatgggga ttttcacaat atccgctggt
     1921 tgccgggcat gaggtgattg ggcgcgtggt ggcactcggg agcgccgcgc aggataaagg
     1981 tttgcaggtc ggtcagcgtg tcgggattgg ctggacggcg cgtagctgtg gtcactgcga
     2041 cgcctgtatt agcggtaatc agatcaactg cgagcaaggt gcggtgccga caattatgaa
     2101 tcgcggtggc tttgccgaga agttgcgtgc ggactggcaa tgggtgattc cactgccaga
     2161 aaatattgat atcgagtccg ccgggccgct gttgtgcggc ggtatcacgg tctttaaacc
     2221 actgttgatg caccatatca ctgctaccag ccgcgttggg gtaattggta ttggcgggct
     2281 ggggcatatc gctataaaac ttctgcatgc aatgggatgc gaggtgacgg cctttagttc
     2341 taatccggcg aaagagcagg aagtactggc gatgggtgcc gataaagtgg tgaatagccg
     2401 cgatccgcag gcactgaaag ccctgtcggg gcagtttgat ctcattatca atactgtgaa
     2461 cgtcagcctc gactggcagc cttattttga ggcgctgacc tatggcggta atttccatac
     2521 ggtcggtgcg gttctcacgc cgctgtctgt tccggccttt acgttaattg cgggcgaccg
     2581 cagcatctct ggttctgcta ccggcacgcc ttatgagctg cgaaagctga tgcgctttgc
     2641 cgcccgcagc aaggttgcgc cgacaaccga actgttcccg atgtcgaaaa ttaacgacgc
     2701 cattcagcat gtacgcgatg gtaaagcgcg ctaccgagta gtcctgaaag ccgacttctg
     2761 atccctttgc cgggtgctgt cgcccggcac ttaaatctct ttggaaggcg tctcgcaatc
     2821 tgctgaattt agcggtgaag agtgcacagc cgttgcctat actccaggca ggaaactgga
     2881 ggaaatctca tgagcgaacc cctgttaatt gcccgcacgc cggacacaga actgttttta
     2941 ctgccgggaa tggctaaccg tcacgggctg attactggcg caacggggac gggtaaaacc
     3001 gttacgctgc aaaaactggc agagtcattg tcggaaatcg gcgtgccggt gtttatggct
     3061 gatgtgaaag gcgatctgac cggtatcgcg caggcaggaa cggcgtcgga aaaactgctc
     3121 acaaggctta aaaatatcgg cgtcaatgac tggcaaccgc atgccaatcc ggtggtggtg
     3181 tgggatatct ttggcgagaa aggccacacg gtgcgggcga cggtttcgga tctggggccg
     3241 ctgttgctgg cgcgactgtt gaatctcaac gatgtgcaat ctggcgtgct gaatattatc
     3301 ttccgcattg cggacgatca gggattgttg ctgctcgact ttaaagattt gcgggcgatt
     3361 acccagtaca tcggcgataa cgccaaatcc ttccagaatc agtacggaaa tatcagtagc
     3421 gcatcggttg gtgccatcca gcgcggatta ctgtcgctgg aacagcaagg cgcagcacac
     3481 ttctttggtg agccgatgct ggatatcaaa gactggatgc gcaccgatgc caacggtaaa
     3541 ggcgttatca atatcctcag cgccgagaaa ctttatcaga tgccgaaact gtacgccgcc
     3601 agcctgctgt ggatgctttc agagttgtat gaacaattgc cggaagcggg cgatctggag
     3661 aaaccaaaac tggtgttttt cttcgacgag gcacatctgc tgtttaacga tgcaccgcag
     3721 gtactgctgg ataagattga gcaggtgata aggcttattc gctcaaaagg cgtgggcgtc
     3781 tggttcgttt cgcaaaaccc gtctgatatt ccggataatg tgctcggcca gctcggtaat
     3841 cgtgttcagc acgctttgcg agcttttacg ccaaaagatc agaaagcagt gaagacagcg
     3901 gcgcaaacca tgcgggccaa tccggcattt gataccgaaa aggcaatcca ggaactgggg
     3961 accggcgagg cgttaatctc gtttctcgat gcaaaaggaa gtccttctgt ggtggaacgg
     4021 gcgatggtga tcgcaccttg ttcgcgaatg gggccggtga cggaagatga gcgtaatggc
     4081 ctgattaatc actccccggt gtatggcaaa tatgaagatg aggtggaccg cgagtccgcc
     4141 tatgagatgc tgcaaaaagg ctttcaggcc agtaccgagc agcaaaataa tccccccgtg
     4201 aaagggaaag aggtggcggt ggatgacggt attcttggtg gattgaagga tattttgttt
     4261 ggcactaccg gaccacgcgg cgggaagaaa gatggtgtgg tgcaaacaat ggcgaaaagc
     4321 gccgctcgcc aggtgacgaa tcagattgta cgcgggatgt tggggagttt gctggggggg
     4381 agaaaaagat aagtcagacg tgatctgtgc ccctcaccct aaccctctcc ccaaaggggc
     4441 gaggggaccg ttcgaactcg tatttgactt tgtcaacaat aataaaggga gctttcgctc
     4501 ccttgttaga ttacgatttt ctcattaaca gccacaggct gattaagaag aagctggcac
     4561 ttggcaacag tgcgccgatg atcggcggga tgccgtaaac caacgtcagc gggccgaaga
     4621 tctggtccag tacgtagaag acaaaaccga agctgatacc tgtgaccaca cgcacgccca
     4681 tcggtacgct acgcagtggg ccaaagataa acgacagcgc catcagcatc atcaccgcca
     4741 cggatagcgg ctggaagatt ttgctccaca tgttgagctg ataacgtccg gcatcctgac
     4801 cgctcgactt cagatacttc acgtagttgt gcaaaccact gatggagagt gcatccgggt
     4861 ccagcgccac cacgcccagt ttgtctggcg tgaggtcggt tttccaggtg ccgctcaccg
     4921 tctgcgaacc ggtaatctgt ttcggattgg tcagatcaga ttcatcaacc tgcgacagac
     4981 gccagacttt atgttccggg tcaaacttcg cggtagcggc ataacgtacg gattgcagac
     5041 gacgattctc gttaaaggca taaatactga tgccacctaa cacctcgtca cctttaaccc
     5101 gctcaatgta gacgaagttg ttgccatctt tcgcccataa accttgctgg gtagagagca
     5161 acgagccgcc gtacatcgcc tgcgcacggt agttacgcgc catctgttcg ccctgcggcg
     5221 cgacccattc gccaatcgcc atcgtcagca agaccagtgg aatggcggtt ttcatcaccg
     5281 acagcgctac ctgcatacgg gtaaaaccag aagcctgcat caccaccagt tcgctgcgct
     5341 gcgccagcat cccaagacca agcaacgccc caagaagagc cgccatcggg aagaagatct
     5401 gcacatcttt cggcacgctc agcaaggtat acattcctgc gcctaacgcg tcgtaactcc
     5461 cctgcccggc ttttttcagc tgatcgacaa atttgataat gcccgacagc gacaccagca
     5521 tgaacagcgt catcatgatg gtggtgaaaa tagttttacc gatatagcgg tcaagtacgc
     5581 caaaaggttg catcacaccg ctcctttacg cgaaaaactg gcgcgcaggc ggcggatcgg
     5641 cacggtgtcc caaaggttga gaacaatcgc caaagccaga taaatcaggt taacggtcca
     5701 catccacagt gtcgggtcca gcttaccttt accgccgttc gatttcaggg aggtctggat
     5761 caggaagaaa agtagataca gcagcatggc tggcagcatc gacagtacgc gtccctgacg
     5821 cgggttaacc acgctcaacg gtacaaccat aagtgccatc ataaacacgg tgaataccaa
     5881 cgtgatacgc cagttcagtt ctgcgcgagc acgatcggtg tcagtgttcc acaatgtgcg
     5941 catgtccatc tggtcggtat cgttcgggtc gagcgccacc gcctggtgac caatgatcgc
     6001 ctgataatcc tggaaatccg taatgcggaa atcacgtaac agtgcagtgc cttcgaagcg
     6061 tgttccctgg ttgagagtga cgacctggga gccgtcgcgc agctgggtta aatgtccgga
     6121 atcggccacc accacagaag gacgtgcatt accttttggt cgaatttgtg cgaggaacac
     6181 atctttgaaa tcgctgccgt caacgctttc gatgaacagc accgagctgc cattagtcgc
     6241 ttgctggaat tgcccttgcg ccagcgccgc catgccaggg ttcgctttcg cttctgctaa
     6301 cacttcatcc tgatgacgcg atgaccacgg tcccgcccac atcacgttaa ccgccgcgac
     6361 gattgccgtg aataccgcaa ggatcattgc cgctttcacc agaaccgctt tgctcaagcc
     6421 gcaggcatgc attaccgtaa tttcactttc ggtatacagt ttgcccagcg tcatcagcag
     6481 cccgaggaac aggcttaatg gcaggataag ctgcgccatt tccggcacgc ccaacccgag
     6541 aagggagagc accagattcg ccggaatatc gccgtcaacc gctgcgccga ggatcctcac
     6601 taacttttga cagaagaaga tcaaaagcaa gatgaagagt atcgccagct ggcttttgag
     6661 cgtctcccgc accagatatc ttatgattat cactttaaat acgcccgtaa aaactcgtct
     6721 tttgcaggat tttagcttgt ttcatggctt aaacgtcatt tattctcttg agtcgtcgaa
     6781 atcgtcgcta agataattat actcaacgga ttcacctctc agattttgtt ctgacgtgcc
     6841 aatgccgtaa taacgttaag attaacacga agtcatcgca acagcggaca tgagttacga
     6901 aagcttgcaa ttctatctgt agccaccgcc gttgtcttta agattcagga gcgtagtgca
     6961 tggagtttag tgtaaaaagc ggtagcccgg agaaacagcg gagtgcctgc atcgtcgtgg
     7021 gcgtcttcga accacgtcgc ctttctccga ttgcagaaca gctcgataaa atcagcgatg
     7081 ggtacatcag cgccctgcta cgccggggcg aactggaagg aaaaccgggg cagacattgt
     7141 tgctgcacca tgttccgaat gtactttccg agcgaattct ccttattggt tgcggcaaag
     7201 aacgtgagct ggatgagcgt cagtacaagc aggttattca gaaaaccatt aatacgctga
     7261 atgatactgg ctcgatggaa gcggtctgct ttctgactga actgcacgtt aaaggccgta
     7321 acaactactg gaaagtgcgt caggctgtcg agacagcaaa agagacgctc tacagtttcg
     7381 atcagttgaa aacgaataag agcgaaccgc gtcgtccgct gcgtaaaatg gtgttcaacg
     7441 tgccgacccg ccgtgaactg accagcggtg agcgcgcgat ccagcacggt ctggcgattg
     7501 ccgccgggat aaaagcagcg aaagatctcg gtaatatgcc gccgaatatc tgtaacgccg
     7561 cttatctcgc ctctcaggcg cgccagctgg ctgacagcta cagcaagaat gtcatcaccc
     7621 gcgttatcgg cgaacagcag atgaaagagc tgggaatgca ctcttatctg gcggtcggtc
     7681 agggttcaca aaacgaatcg ctgatgtcgg tgattgagta caaaggcaac gcgtcggaag
     7741 atgcacgccc aatcgtgctg gtgggtaaag gtttaacctt cgactccggc ggtatctcca
     7801 tcaagccttc agaaggcatg gatgagatga agtacgatat gtgcggcgcg gcggcggttt
     7861 acggcgtgat gcgtatggtc gcggagctgc aactgccgat taacgttatt ggcgtgctgg
     7921 caggctgtga aaacatgcct ggcgggcgtg cctatcgtcc gggcgatgtg ttaaccacca
     7981 tgtccggtca aaccgttgaa gtgctgaaca ccgatgccga aggccgcctg gtactgtgcg
     8041 acgtgttaac ttacgttgaa cgttttgagc cggaagcagt gattgacgtg gcgacgctga
     8101 ccggtgcctg cgtgatcgcg ctgggtcatc atattactgg tctgatggcg aaccataatc
     8161 cgctggccca tgaactgatt gccgcgtctg aacaatccgg tgaccgcgca tggcgcttac
     8221 cgctgggtga cgagtatcag gaacagctgg agtccaattt tgccgatatg gcgaacattg
     8281 gcggtcgtcc tggtggggcg attaccgcag gttgcttcct gtcacgcttt acccgtaagt
     8341 ataactgggc gcacctggat attgcaggaa ccgcctggcg ttctggtaaa gcaaaaggcg
     8401 caaccggtcg tccggtagcg ttgctggcac agttcctgtt aaaccgcgct gggtttaacg
     8461 gcgaagagta agtttgcgtt ttaccctcac cctaaccctc tccctaaggg agagggaacc
     8521 gatcgagcgc aatagaaatt cattgctcat ctttcagggt gaggaatgca gcaatattta
     8581 aatccaccac aagaagcccc atatatgaaa aacgcaacgt tctaccttct ggacaatgac
     8641 accaccgtcg atggcttaag cgccgttgag caactggtgt gtgaaattgc cgcagaacgt
     8701 tggcgcagcg gtaagcgcgt actcatcgcc tgtgaagatg aaaagcaggc ttaccggctg
     8761 gatgaagccc tgtgggcgcg tccggcagaa agctttgttc cgcataattt agcgggagaa
     8821 ggaccgcgcg gcggtgcgcc ggtggagatc gcctggccgc aaaaacgcag cagcagcccg
     8881 cgcgatatat tgattagtct gcgaacaagc tttgcagatt ttgccaccgc tttcacagaa
     8941 gtggtagact tcgttcctta tgaagattct ctgaaacaac tggcgcgcga acgctataaa
     9001 gcctaccgcg tggctggttt caacctgaat acggcaacct ggaaataatg gaaaagacat
     9061 acaacccaca agatatcgaa cagccgcttt acgagcactg ggaaaagcag ggctacttta
     9121 agcctaatgg cgatgaaagc caggaaagtt tctgcatcat gatcccgccg ccgaacgtca
     9181 ccggcagttt gcatatgggt cacgccttcc agcaaaccat catggatacc atgatccgct
     9241 atcagcgcat gcagggcaaa aacaccctgt ggcaggtcgg tactgaccac gccgggatcg
     9301 ctacccagat ggtcgttgag cgcaagattg ccgcagaaga aggtaaaacc cgtcacgact
     9361 acggccgcga agctttcatc gacaaaatct gggaatggaa agcggaatct ggcggcacca
     9421 ttacccgtca gatgcgccgt ctcggcaact ccgtcgactg ggagcgtgaa cgcttcacca
     9481 tggacgaagg cctgtccaat gcggtgaaag aagttttcgt tcgtctgtat aaagaagacc
     9541 tgatttaccg tggcaagcgc ctggtaaact gggacccgaa actgcgtacc gctatttctg
     9601 acctggaagt ggaaaaccgc gaatcgaaag gttcgatgtg gcacatccgc tatccgctgg
     9661 ctgacggcgc gaaaaccgca gacggtaaag attatctggt ggttgcgacc actcgcccgg
     9721 aaaccctgct gggcgatacc ggtgtagctg ttaacccgga agatccgcgt tacaaagatc
     9781 tgattggcaa atatgtcatt ctgccgctgg ttaaccgtcg tattccgatc gttggcgacg
     9841 aacacgccga catggaaaaa ggcaccggct gcgtgaagat caccccggcg cacgacttta
     9901 acgactatga agtcggtaaa cgtcacgccc tgccgatgat caacatcctg acctttgacg
     9961 gtgatatccg tgaaagcgcc caggtgttcg ataccaaagg taacgaatct gacgtttatt
    10021 ccagcgagat cccggcagag ttccagaaac tcgagcgttt tgctgcacgt aaagcagtcg
    10081 ttgctgcggt tgatgcactc ggcctgctgg aagaaattaa accgcacgac ctgaccgttc
    10141 cttacggcga ccgtggcggc gtggttatcg aaccgatgct gaccgaccag tggtacgtgc
    10201 gtgccgatgt actggcgaaa ccggcagttg aagcggttga gaacggcgac attcagttcg
    10261 taccgaagca gtacgaaaac atgtacttct cctggatgcg cgatattcag gactggtgta
    10321 tctctcgtca gctgtggtgg ggtcaccgta tcccggcatg gtatgacgaa gcgggtaatg
    10381 tttatgttgg ccgcaacgaa gaagaagtgc gcaaggaaaa taacctcggt gccaacgttg
    10441 ccctgcgtca ggacgaagac gttctcgaca cctggttctc ttctgcactg tggaccttct
    10501 ctacccttgg ctggccggaa aataccgacg ccctgcgtca gttccaccca accagcgtga
    10561 tggtatctgg tttcgacatc atcttcttct ggattgcccg catgatcatg atgaccatgc
    10621 acttcatcaa agatgaaaat ggcaaaccgc aggtgccgtt ccacactgtt tacatgaccg
    10681 gtctgattcg tgacgacgaa ggtcagaaga tgtccaaatc caagggtaac gttatcgacc
    10741 cgctggatat ggttgacggt atttcgctgc cagaactgct ggaaaaacgc accggcaata
    10801 tgatgcagcc gcagctggcg gacaaaatcc gtaagcgcac cgagaaacag ttcccgaacg
    10861 gcattgagcc gcacggcacc gatgccctgc gcttcaccct ggcggcgctg gcgtctaccg
    10921 gtcgtgacat caactgggat atgaagcgtc tggaaggcta ccgtaacttc tgtaacaagc
    10981 tgtggaacgc cagccgcttt gtgctgatga acacagaagg tcaggattgc ggcttcaacg
    11041 gcggcgaaat gacgctatcg ctggcggacc gctggattct ggcggagttc aaccagacta
    11101 tcaaagcgta ccgcgaagcg ctggacagct tccgcttcga tatcgccgca ggcattttgt
    11161 atgagttcac ctggaaccag ttctgcgact ggtatctgga gctgaccaag ccggtaatga
    11221 acggtggaac agaagccgaa ctgcgcggta ctcgccatac gctggtgact gtactggaag
    11281 gtctgctgcg cctcgcgcat ccgatcattc cgttcatcac cgaaaccatc tggcagcgtg
    11341 tgaaagtact ttgcggtatc actgccgaca ccatcatgct gcagccgttc ccgcagtatg
    11401 atgcatctca ggttgatgaa gccgcactgg ccgacaccga gtggctgaaa caggcgatcg
    11461 ttgctgtacg taacatccgt gcagaaatga acatcgcgcc gggcaaaccg ctggagctgc
    11521 tgctgcgtgg ttgcagcgcg gatgcagaac gtcgcgtaaa tgaaaaccgt ggcttcctgc
    11581 aaacgctggc gcgtctggaa agcatcaccg tgctgcctgc cgatgacaaa ggtccggttt
    11641 ccgttaccaa gatcatcgac ggcgcagagc tgctgatccc gatggctggc ctcatcaaca
    11701 aagaagatga gctggcgcgc ctggcgaaag aagtggcgaa gatcgaaggt gaaatcagcc
    11761 gcatcgagaa caagctggca aatgaaggct ttgttgcccg cgcaccggaa gcggttatcg
    11821 cgaaagagcg tgagaagctg gaaggttatg cagaagcgaa agccaagctg attgaacagc
    11881 aggctgttat cgccgcactg taatcaacac agtgatgaaa acgaaggccg gagcatgctc
    11941 cggccttttt tatctcttac agaaatggta aagaaggcat gactccccga gaaatactgg
    12001 ctaagaagcc tttatctggt tgttcttctt tattgattaa aggaaggtca tccaaatcgc
    12061 ccagcaaata cgtatttttt gcctgccagt caatcatcca tattttcagc agtgggtaag
    12121 ccagaccacc cgtaatcccg gatatcacca ccaacgcaca catgcgataa agcataccgt
    12181 ggtacgttaa agttgagcga aaacgaatac gcccatcatt cagtgacagg ttgctcataa
    12241 aatgatttcg caacgacacc gttaaataac tggtgctaac agcaatccca aaataataaa
    12301 ttaactgcgc gattatcatt ttacgttgca tttccataaa ttgctgtaaa ttctcaatat
    12361 catcatttgc atacccagaa ctatcatact catttaatat ttgatcgaag ataatataac
    12421 cagcaacagc gagaaaaggt aataaatcta aaaatgtgag gatggcatat tttatacaat
    12481 acgcagtatc taatttcacc ttgaaacgat gtataccgaa actggtattg ctccagagaa
    12541 aactcattac caggctatat aaagtaccat taaaaatacc aatggaaaca ataccaacta
    12601 ttgccatcag aaccacagat ataataacac tacttgaact attggcatgt agcatctttg
    12661 tcgagataaa gaaaacagtc cccatcccaa tggccattaa aatcggcaag aaaaaggtta
    12721 cccaccagaa ccctttcaaa gagcaattaa aactaaatct tacgctgttg agactggtca
    12781 ttaaggcctg ataacgcagt ccttttgctg ccataaatat aagcaaaacc aacagtaata
    12841 aagtcaaaac acagccaaca agcggcatac ctgctgacac tgtcattaag attgcgaaat
    12901 agaaaaaaac aaaaacaaga caactaacaa aaacattccc accggtaatt ccataagaaa
    12961 atcgttgtcc gttaacttcc atattagcat aaagataacg cttacatttc attaatgccc
    13021 atggtaaata gatacctagc gttataatcg ttaacaacac attcaccaca caaataagaa
    13081 aatatctctc acctgtacca tgaaaaacaa aagaatggga cggaacatcc atttcattaa
    13141 taacttgagc cataaaaatc ccctgaatta aaaaatccat tgatataatt tatagataca
    13201 gacagtaaaa tgttctgcca cgttacaata ctcttatcag taataggctg gcaaaaactt
    13261 tattgcttta taagacgctt gcgcacgtaa gaagagagtg gtattaaaca aatgtataat
    13321 tactttattt ttattgggtt aaactatgaa taacattgcg ccgcaatcac ctgtaatgcg
    13381 tcgcctgacg ctgcaggata atcctgctat cgccagcgtc atccgtcagg tttacgcaga
    13441 gtatggtctt accgccgata aaggctacac cgttgccgat ccgaatctcg acgaactgta
    13501 tcaagtgtac agccagcctg gccatgctta ttgggtggtt gaatacaatg gcgaagtggt
    13561 cggcggcggc gggattgcgc cattaacagg tagtgaagcg gacatttgcg aactgcagaa
    13621 aatgtacttt cttcccgcta ttcgcggtaa agggctagcg aaaaaactgg ccttaatggc
    13681 gatggagcag gctcgagaga tgggcttcaa acgctgctat ctggaaacga ccgctttttt
    13741 aaaggaagcc attgcgcttt atgagcattt gggctttgag catatcgact atgcgcttgg
    13801 ctgcacgggc catgtcgatt gtgaagtgcg gatgctacgc gaactctaaa aaaatgcccg
    13861 ctagtatgtg ctatcgggca ttttaatcaa ttaattagtg gcgaaccccg tcatcgtctt
    13921 catcgacaaa atcttcatcg tcgccatctt cgccgttagg atcttcaaag taagtgcccc
    13981 aaccgtcgta ctcaacgtca aatttctccg ccagcgtcat cagttgttca acctgggcat
    14041 cgatcagatc ggcattcaac gcgcactcgc tgaggatgtc gcagcaaatc acgatatcac
    14101 catcttcaac ttccagctct tctggatcgg tcacttcgta accgagttta aacgcttcaa
    14161 ctgccgcttt ttccagagtt tccagatcgt ctgcggaaag atggtgttcg atggtgtaca
    14221 gcgcgtccgg atcgctgcca tcttccagta attcttcaat aatcaaacgt gtttcttcac
    14281 gctgttcttc cagttgttcc gggtttgcca tggctcattc ctcttaatgt cctgccgata
    14341 ctcttattgt cacacaccac tgacattgcc tccacctttg tgacaaagat ttatgcttta
    14401 gacttgcaaa tgaataatca tacacataaa tttaatttta attcattgag gcgttagcca
    14461 caggagggat ctatgtccgg gttttatcat aagcatttcc tgaaattact cgatttcacg
    14521 ccagctgaac tcaacagcct gctgcagtta gccgcgaagc tgaaagctga taagaaaagc
    14581 ggtaaagaag aagccaaact cactggtaaa aacatcgcgc tcatcttcga aaaagactcg
    14641 actcgtaccc gatgctcttt cgaagttgcc gcatatgacc agggtgctcg cgttacttat
    14701 cttggcccaa gcggtagtca gattggtcat aaagagtcga ttaaagacac tgcccgcgtg
    14761 cttggtcgca tgtatgacgg tattcagtat cgcggctatg gtcaggagat tgtcgaaaca
    14821 ctggcggaat acgctggcgt gccggtatgg aacggcctga ccaatgagtt tcaccccacg
    14881 cagttgctgg cggatctcct caccatgcag gagcatttac ccggtaaagc attcaacgaa
    14941 atgacgctgg tctatgcagg tgacgcacgt aacaacatgg gcaattcgat gctcgaagct
    15001 gcggcgctta ccggtctgga tttgcgtctg gtcgcgccac aggcatgctg gccagaagct
    15061 gcgctggtta cggaatgccg cgccctggca caacaaaatg gcgggaatat tacgctgacg
    15121 gaagatgtag ctaaaggagt tgaaggtgct gactttatct ataccgatgt atgggtgtcg
    15181 atgggtgaag caaaagaaaa atgggctgaa cgcattgcat tactgcgtga ttatcaggtg
    15241 aacagcaaga tgatgcagtt gaccggtaac ccggaggtca aattcctcca ctgcctgccc
    15301 gcgtttcatg acgaccaaac gacgcttggc aaaaaaatgg cggaagagtt tggcctacat
    15361 ggcggaatgg aagtgactga tgaggtcttc gagtctgccg ccagcattgt atttgatcag
    15421 gcggaaaacc gcatgcatac catcaaagcg gtgatggtcg cgacgctcag taaattaaat
    15481 aattaata
//
LOCUS       DAJTXY010000094        15097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTXY010000094 DAJTXY010000000
VERSION     DAJTXY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.094"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            43..306
                     /locus_tag="OO566_002976"
     CDS             43..306
                     /locus_tag="OO566_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR6606189.1"
                     /translation="MSKLESLPTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLV
                     FAGVRLYMPALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            316..954
                     /locus_tag="OO566_002977"
     CDS             316..954
                     /locus_tag="OO566_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6606190.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLGRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            957..2120
                     /locus_tag="OO566_002978"
     CDS             957..2120
                     /locus_tag="OO566_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6606191.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2204..3828
                     /locus_tag="OO566_002979"
                     /pseudo
     CDS             2204..3828
                     /locus_tag="OO566_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3945..4220)
                     /gene="yjfN"
                     /locus_tag="OO566_002980"
     CDS             complement(3945..4220)
                     /gene="yjfN"
                     /locus_tag="OO566_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6606192.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4369..4698)
                     /gene="bsmA"
                     /locus_tag="OO566_002981"
     CDS             complement(4369..4698)
                     /gene="bsmA"
                     /locus_tag="OO566_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6606193.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4880..5629
                     /gene="yjfP"
                     /locus_tag="OO566_002982"
     CDS             4880..5629
                     /gene="yjfP"
                     /locus_tag="OO566_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6606194.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5626..6381)
                     /gene="ulaR"
                     /locus_tag="OO566_002983"
     CDS             complement(5626..6381)
                     /gene="ulaR"
                     /locus_tag="OO566_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6606195.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6489..7553)
                     /gene="ulaG"
                     /locus_tag="OO566_002984"
     CDS             complement(6489..7553)
                     /gene="ulaG"
                     /locus_tag="OO566_002984"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6606196.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7908..9305
                     /gene="ulaA"
                     /locus_tag="OO566_002985"
     CDS             7908..9305
                     /gene="ulaA"
                     /locus_tag="OO566_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6606197.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9321..9626
                     /gene="ulaB"
                     /locus_tag="OO566_002986"
     CDS             9321..9626
                     /gene="ulaB"
                     /locus_tag="OO566_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6606198.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9636..10100
                     /gene="ulaC"
                     /locus_tag="OO566_002987"
                     /pseudo
     CDS             9636..10100
                     /gene="ulaC"
                     /locus_tag="OO566_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
     gene            10114..10764
                     /gene="ulaD"
                     /locus_tag="OO566_002988"
     CDS             10114..10764
                     /gene="ulaD"
                     /locus_tag="OO566_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6606199.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10774..11610
                     /gene="ulaE"
                     /locus_tag="OO566_002989"
     CDS             10774..11610
                     /gene="ulaE"
                     /locus_tag="OO566_002989"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6606200.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11610..12296
                     /gene="ulaF"
                     /locus_tag="OO566_002990"
     CDS             11610..12296
                     /gene="ulaF"
                     /locus_tag="OO566_002990"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6606201.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12425..12700)
                     /locus_tag="OO566_002991"
     CDS             complement(12425..12700)
                     /locus_tag="OO566_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6606202.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13027..13422
                     /gene="rpsF"
                     /locus_tag="OO566_002992"
     CDS             13027..13422
                     /gene="rpsF"
                     /locus_tag="OO566_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6606203.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13429..13743
                     /gene="priB"
                     /locus_tag="OO566_002993"
     CDS             13429..13743
                     /gene="priB"
                     /locus_tag="OO566_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6606204.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13748..13975
                     /gene="rpsR"
                     /locus_tag="OO566_002994"
     CDS             13748..13975
                     /gene="rpsR"
                     /locus_tag="OO566_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6606205.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14017..14466
                     /gene="rplI"
                     /locus_tag="OO566_002995"
     CDS             14017..14466
                     /gene="rplI"
                     /locus_tag="OO566_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6606206.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14537..>15097)
                     /locus_tag="OO566_002996"
     CDS             complement(14537..>15097)
                     /locus_tag="OO566_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6606207.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     RTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3533 a         3676 c         4063 g         3825 t
ORIGIN      
        1 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca
       61 ttacctacat tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt
      121 attccagact atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct
      181 ggtgtcagac tttatatgcc cgcattaagc gaaaaaatta ttaatcacaa taccgcagca
      241 ggaatgttta tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca
      301 tggtaatgga cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac
      361 ggagcgatca gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatcgctac
      421 gcacaaaaat acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt
      481 tgtagcgata gtagcgatgt cgataacgac ggcgacggaa cgttttacgc gacagtgcag
      541 gattgtattg atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa
      601 gcggcatttt atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac
      661 gaaaattgct actacgacaa tgttgaacag agttggatcc cggtcgtttc tggatttttg
      721 ttaggccggg ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc
      781 tcatttgctt cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt
      841 tctggcaaaa aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt
      901 tcgcgcggcg gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc
      961 tgagacacaa cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg
     1021 actttcatat catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc
     1081 tgcgccagat tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg
     1141 aggtggtgga tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt
     1201 tgtactggga tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga
     1261 tggattttgc ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc
     1321 cgacctcgtt gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca
     1381 gcggcattat tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc
     1441 gctttagtga gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg
     1501 aagacaggag taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc
     1561 ggtttatcta cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg
     1621 ataatgtcat ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata
     1681 acggtccgct gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct
     1741 tgagtaataa agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc
     1801 ttgcatcctg gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta
     1861 aaccgatcta ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg
     1921 ttgaccacgc tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc
     1981 tgccgcgatt tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt
     2041 gcggaatggg gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc
     2101 cgcattacat tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca
     2161 gtgaccatac taatggtgac tgccattgat ggagggagac acagtgcact ggcaaactca
     2221 caccattttt aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc
     2281 gctttgcgaa gcggtaacgc gtgaaggtgc tggctgggat agcaattttc tagccagtat
     2341 tggtcagcag ttaggaacgg ctgaatccct tgaactgggg cggctggcga atgtaaaccc
     2401 gcctgaactg ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc
     2461 cgcatggcac ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga
     2521 agaagacgct cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatggcag
     2581 gttgaggctg ggtcgttatg tccaataacg atgacctttg ccgccacgcc attgttgtta
     2641 cagatgttac ccacgccgtt tcaggactgg accacgccgc tgctgagcga tcgctacgat
     2701 tctcacttat tgccaggtgg gcaaaaacgc ggtttgttga ttggcatggg aatgacggaa
     2761 aagcagggcg gttccgatgt catgagcaac accatccgtg cagagcgttt ggaagatggc
     2821 tcttatcggc tggtggggca taaatggttt ttctcggtgc cgcaaagcga tgcgcatctg
     2881 gtgctggcgc agaccgcggg cggtctgtcc tgcttttttg tgccgcgctt tttgcctgac
     2941 gggcaacgca acgcgattcg cctcgagcgg ctgaaagata agctgggtaa tcgctctaac
     3001 gccagttgcg aagtggagtt tcaggatgcc attggctggt tattggggca ggaaggggaa
     3061 ggaattcgtc tgatcctgaa aatggggggg atgacgcgtt ttgattgcgc cctgggtagc
     3121 catgccatga tgcgccgtgc attttcgctg gcgatttatc atgcacatca acgccatgtt
     3181 tttggtaatc cattgatcca acagcccctt atgcgtcatg tcttaagtcg catggcactt
     3241 cagcttgaag ggcaaacggc gttgctgttt cgtcttgcgc gagcgtggga ccggcgtgcc
     3301 gatgccaaag aagccctgtg ggcgcgttta tttacgcctg cggcaaaatt tgtgatctgc
     3361 aaacgaggta tgccgtttgt ggccgaagcg atggaggtgc tgggcggcat tggttattgc
     3421 gaggagagcg agctgccgcg gctttaccgg gagatgccgg taaacagtat ttgggaaggt
     3481 tccggcaata ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc gggcgtatac
     3541 gacttattgt cggaagcatt tgtggaagtg aaagggcagg atcgctattt tgatcgcgcg
     3601 gttcgtcgtt tacagcagca gctgcgtaag ccagctgaag aactggggcg agagattact
     3661 catcagctat tcctgctggg ctgcggtgcg caaatgttga aatatgcttc tccgccaatg
     3721 gcgcaggcgt ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag
     3781 atccagaatg atttattgct gcgggcgacg gggggagtgt gtgtgtaagc gtatacgact
     3841 gatgcgacgc tggtttcgat taactaaatg aaatatgtga aaattgtagg ccggacaagg
     3901 cgctcgcgcc gcatccggca ttgttcattt gctgtagatg acactcatgc atacagtatc
     3961 gcctgtacgc gccagttatc aggctgctgg tcttcgtaca tggtaataat gcggtaccat
     4021 gacgcacctt gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg cggatcccct
     4081 gaaatattgc taactgagat ctgacctatt tcattcaggc ccgtcacgca gtcagcactg
     4141 gcgaattctg cagactgtgc cgtggcgctc gcaggataag tcactaattg cagcgagggt
     4201 gaggcaagta attgtttcat cgtcagctcc atttttcgtt tccccacatg ttgcagggca
     4261 ttccatcgcg gtaataaacg cccaagcttc cagccggtaa tttattgtgc acaggtaaac
     4321 gcatagggac gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt atttacggta
     4381 caaaatggct tgtgaatacc actgtcctgt cacgatggtt tcgtccacca tgacgacaac
     4441 gtaataatca gcttttgcag cgacagcttt tgctctgatt tctgctaatg cgtcatccgg
     4501 agaaccccga accatcgtgc ttacgctacc tattcgctgt aacccttgcg tctggtcgcg
     4561 gcgaatctct tgcggatggt ccgttactgg cggtgctggc tgtggcgtac cttgcagtgc
     4621 gctacaggca cttaacatca acaccaataa taaactggca aaccggtaaa taacgctatt
     4681 acgtttcctg ctaaccatag tgtagtgcct tattaattta actttggggg aatgttcccg
     4741 aattgttacc tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa cccacatctc
     4801 aacattatga actaataccg ccagcagaaa cttgtgctaa ggatttcttc atgtcatacc
     4861 agacaaaaag gagagacaga tgattgaaat agaatcacgc gagctggcag atattcccgt
     4921 tcttcatgct tatcctgtcg ggcaaaaaga taccccgtta ccgtgcgtaa ttttttatca
     4981 cggctttact tcatccagcc tggtgtatag ctattttgcc gttgcgctgg cgcaggctgg
     5041 tttgcgggtg atcatgccgg atgcgcccga tcacggtagc cgttttagtg gcgacgaagc
     5101 gcggcggtta aatcaattct ggcaaatctt gctacaaagt atgcaggaat tcactacttt
     5161 acgtgcggca atagccgaag aaaactggct gcttgatgac cgtctggcag tcggtggcgc
     5221 gtcgatgggc gcgatgacgg cactgggaat taccgctcgc caccccacgg tgaaatgtac
     5281 cgccagcatg atgggatcgg gctattttac atcgctcgcc cgttcactgt ttccaccgct
     5341 gatacctgaa acggcagcac agcagaatga attcaataaa attgtcgcgc cgctggcaga
     5401 gtgggaagcg acaaaccacc tagaacaact tggggacaga cctctactgc tgtggcatgg
     5461 cctcgacgat gacgttgtgc ctgccgacga atcactacgt ttgcagcagg ccttaagcga
     5521 gacgggacgg gataaactgc taacctgttc atggcagcca ggcgtgcgtc accgcattac
     5581 gcctgaggcg ttagatgctg ccgtgacatt tttccgccag catctttaaa cacgcagaat
     5641 gctgacccct tgcgcttcca gttgttgcag gatttccggg ttagcatttt tgccggtgat
     5701 cagcatatcg atttgatcgg cacggctaaa aagcattccc gcgcgttcgc caatcttact
     5761 gctatcaacc agtaccacca gtttccctac cacgctcagc atcttctgct ctgccattgc
     5821 tgtcagcata tcggttttat acaacccttc tgcggtcagc ccttttccgc tggtaaacat
     5881 ccagtgcccg gcatagagac tgttttcgct gccctgcggg cttaaagtga tggactgact
     5941 tttgttgtac tgtccaccca taatgatcac gctgtcatgt tcttgatcga tcaggtaatt
     6001 tgccagcggt agataattag tgatgatttg cactggcttg ccacacattt cccgcccaag
     6061 cagaaacgcg gtggagccgc agttgatgac tacgctttcg cccggattaa ccagctgcga
     6121 ggccgcttta gcaatacgta ctttttcatc gtgattctgc gcctgatgca gattcatcgg
     6181 cgtccagcgc gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct ttttcagttt
     6241 gccgctttcg ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa ttcccagacg
     6301 ctcaacgact ttctcaacgg tcacaaagcc caattgtgcg agcatttcca ggaggatttg
     6361 atgtctttgt gcttcagtca tgagctattc cgataaaatt tgattttttt agatgatatt
     6421 tgaaatagcc aggaaatact acgccggata gcgcgaactt ctccacgcta tccggtataa
     6481 acgacagatt acaggaatga cttgaacggc agatccggtt caatagtgaa gcaatcatcg
     6541 aaaccgcgcg gatagtggta ctcgaagttg tctttatcca gcggccaggt aaatttgccg
     6601 ccaacctgcc agataaacgg cttgaagcca tacttcagac gatctttttt catctcccac
     6661 agcacgcgga tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg gtggaacggg
     6721 ataaccactt tcgcattcag ggcttcaccc atacgcagca tatcggcgct ggtcattttg
     6781 tcggtaatac cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat ctgatgttcg
     6841 ttaccgtgct tcgcataata gttagagtag tgggaatcgc cactgtgata tagggaaccg
     6901 ccaggggttt tgaacaggta gtttactgcg cgatcgtcca tgccatctgg cagtacgcca
     6961 gccgcttttt gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc gtcaagcgca
     7021 tgaatttcaa tgtctttcac ttttacaaca tcgcccggtt tgaccacgat gcaacgctct
     7081 ttcggtacgc cccagccaat ccacaaatca acgcaggttt tcggtccaat gaatggtacg
     7141 tcatcagcac agttctgcat cacggcagca gcgacgttaa cgtcgatatg atcgttgtga
     7201 tcgtgagtcg ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag aacaaacggg
     7261 gtggtgcgta ggtttggctg cagttttttc acgccagcca tgcgctgcat ctggtgaccc
     7321 tgtttcatta acgggttacc gtgactttgt ttgccagtgc cgcaccagaa atcaacgcaa
     7381 acgttggtgc caccttctga tttcaaccag atcccggtgc agcctagcca ccacatcgca
     7441 aatgtgccag gggcgacctg ctcttgttca atttcttcat tcagccagct accccactcc
     7501 gggaaagtgc tcaggatcca ggattctcgg gtgatacttt tcactttact catcgccatt
     7561 taccttcatg atagttcaat tcgaatcaat atgtgattgg ttttgattaa tcctgacact
     7621 attttttcag gaaggcaatg accatttttt gactttttgc cagggaagtt gttgttgatt
     7681 ttgagtgtag aaagatttaa tggaatgtat agttaaatta actaaatgaa tttaaataaa
     7741 taattttttc gttgtgtgaa tcccactcta tccgtgtgga attatttgcg ggtcgcgtca
     7801 catttaatca taaataatct tgttgtgatt acttttgaaa attagagtga gtgcacaaca
     7861 ttccgggtgt gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct
     7921 acaacatctt taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta
     7981 ttgtgacctg tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca
     8041 cgattaaaac cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca
     8101 ccttcaaacc ggtggtggcg aaaatgtccg aagtctacgg cattaacggc gcaatttccg
     8161 atacttacgc gtcgatgatg gcaaccatcg accgcatggg cgatgcctat agctgggtag
     8221 gttacgcggt gttgttagcg ctggcgctga acatctgtta cgtgctgctg cgtcgcatta
     8281 ccggtattcg taccatcatg ctgactggtc atatcatgtt ccagcaggcg gggctgattg
     8341 ccgttacgtt gtttattttc ggctactcca tgtggacaac cattatctgc acggcgattc
     8401 tggtttcgct ctactggggc attacctcca acatgatgta caagccgact caggaagtga
     8461 cggacggctg cggtttctcc atcggtcacc agcaacagtt tgcatcatta attgcctata
     8521 aagttgcgcc gttcctcggc aaaaaagagg aaagcgttga agacctcaaa ctgccaggct
     8581 ggctgaatat tttccacgac aacatcgtct ccacggcgat tgtcatgacc atcttctttg
     8641 gtgccattct gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc aaagtgcact
     8701 ggacggtcta tatcctgcaa accggttttt ccttcgcggt ggcgatcttc atcatcactc
     8761 agggtgtgcg catgtttgtg gcggaactct ctgaagcgtt taacggtatc tcccagcgcc
     8821 tgatccctgg tgcggttctg gcgattgact gtgcggctat ttatagcttc gctccgaacg
     8881 ccgtggtctg gggctttatg tggggcacca tcggtcagct gattgcggtt ggcatcctgg
     8941 tcgcctgcgg ttcctcgatc ctgattatcc caggctttat cccgatgttc ttctccaacg
     9001 ccaccatcgg cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg aagatttgtc
     9061 tggtgatggg gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt accggtatga
     9121 gtgcctggat ggggatggcg gactggtcga ttctggcacc gccgatgatg cagggcttct
     9181 tctccatcgg tatcgccttt atggccgtca tcattgtaat tgcactggct tatatgttct
     9241 tcgctggccg cgcactgcgc gcagaagaag atgcagaaaa acaattggca gaacagtctg
     9301 cttaataagg agttttgatt atgaccgtac gtattctggc tgtgtgtggc aacggacaag
     9361 gcagttccat gatcatgaag atgaaagtgg accagttttt aacccaatca aacattgacc
     9421 acacggtaaa tagctgcgcg gttggcgaat acaaaagcga gttgagcggc gcggatatca
     9481 tcatcgcttc tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa tacgtggttg
     9541 gcgtgcgcaa catgctctct cctgccgact ttggcccgaa actgctggaa gtgatcaaag
     9601 cgcatttccc gcaggatgtg aagtaaggac gcgccatgaa attacatgat tcgctggcgg
     9661 agaataaatc catccgcctg caggctgaag ctgagacatg gcaggacgca gtaaaaatcg
     9721 gcgttgacct gctggtggcg gcggatgttg tcgagccgcg ttactaccag gcgattctgg
     9781 atgcggttga acagcacggt ccgtactttg tgctcgcacc aggcctggca atgccgcacg
     9841 gtcgcccgga agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg aaaaagccgc
     9901 tggagttcaa ccacgaagat aacgacccgg tggatatcct catcaccatg gcggtggtcg
     9961 atgccaacac gcactaggaa gtgggcatca tgcagatcgt caacctgttt gaagatgaag
    10021 aaaatttcga ccgtttacgt gcctgccgta ccgagcagga agtactggat ctcattgacc
    10081 gcaccaacgc ggcagcttaa gaaggaattg aagatgtcat taccgatgtt gcaagtcgcg
    10141 ctggacaacc agactatgga tagcgcctac gaaaccactc gcctgattgc cgaagaagtc
    10201 gacattatcg aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc ggttcgcgat
    10261 ctgaaagcgc tctacccgca caaaatcgtg ctggcagacg ccaaaattgc cgatgcgggc
    10321 aaaattcttt ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt gatttgttgt
    10381 gcggatatca acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa cggcgacgtg
    10441 cagatcgaac tgaccggtta ctggacctgg gaacaggcgc aacagtggcg cgacgcaggt
    10501 attcagcagg tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt ggcgtggggt
    10561 gaagccgata tcactgcgat caaacgtctt tccgatatgg gcttcaaagt caccgtcacc
    10621 ggtggcctgg cgctggaaga tctgccgctg ttcaaaggca ttccaattca tgtgtttatc
    10681 gccggtcgca gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca gttcaaacgt
    10741 tccatcgccg aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat cccgcttggc
    10801 atctatgaaa aagcgctccc cgccggggag tgctggctgg aacgcctgcg actggcaaaa
    10861 acgttgggct tcgattttgt cgagatgtcg gtagatgaaa ctgacgagcg cctgtcgcgc
    10921 ctcgactgga gccgcgagca gcgtctggcg ctggtgaatg cgattgttga aaccggcgtg
    10981 cgtgtgccgt ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag cgaagatgac
    11041 gcggtgcggg cgcaggggct ggagattatg cgtaaagcca tccagttcgc ccaggacgtt
    11101 ggcattcgcg tgatccagct ggcgggctat gacgtttact atcaggaagc caataacgaa
    11161 acgcgtcgtc gtttccgtga cggcctgaaa gagagcgttg agatggcgag ccgcgcacag
    11221 gtgacgctgg cgatggagat catggattat ccgttgatga actccatcag caaggcgctg
    11281 ggttacgcgc actatctcaa caacccgtgg ttccagctct acccggatat cggcaacctg
    11341 tcggcgtggg acaacgatgt gcagatggag ttgcaggccg gaatcgggca tatcgtcgcg
    11401 gtgcatgtga aagacaccaa accgggcgtc ttcaaaaacg tgccatttgg cgaaggtgtg
    11461 gtggatttcg aacgttgctt cgaaacgctc aaacagagtg gctattgcgg gccgtacctg
    11521 attgagatgt ggagcgaaac ggcggaagac ccggcggcag aagtggtgaa agcgcgcatg
    11581 gccaaagcag gcatggtgga ggcggcataa tgcaaaagct aaaacagcag gtgtttgaag
    11641 ccaacatgga tctgccgcgc tatgggctgg tgacctttac ttggggcaac gtcagcgcta
    11701 tcgaccgcga acgcgggctg gtggtgatca agcccagcgg cgttgcctac gaaaccatga
    11761 aagcagacga tatggtggtg gtcgatatga gcggcaaggt ggtggaaggg gagtatcgcc
    11821 catcttccga cactgcgacg catctcgaac tctaccgtcg ttacccgtcg cttggcggca
    11881 ttgtccatac ccactccact catgccaccg catgggcgca ggcggggctg gcgatcccgg
    11941 cattaggcac cacgcacgct gactacttct ttggtgatat cccgtgtacg cgtggattaa
    12001 gcgaagaaga ggtgcagggt gagtatgaac tgaacactgg caaagtgatt atcgaaacgc
    12061 tgggcaacgc cgagccgctg catacgccgg gaattgtggt gtatcagcac ggaccgttcg
    12121 cctgggggaa agatgcccac gacgcggtgc ataacgcggt agtgatggaa gaagtggcga
    12181 aaatggcgtg gattgcgcgc agtattaatc cacaactcaa tcatatcgac agctacctga
    12241 tgaataaaca cttcatgcgt aagcacggtc ctaacgctta ttacgggcag aagtaaaaca
    12301 cgcgctgcgg aaatttcctt cctcgggaga taactggtct aattccgcag ccgtttttca
    12361 aaaaaagccc cctgcgaagg gggcaaagca aactatggca atgtttcgtt ggttatacct
    12421 ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc gttactcccc ggttcccgca
    12481 tcagaattac gtggtagtac gttgcgcctt gcgcatctgt ttcttcattt aatgcctgac
    12541 gtgcttcgct ttcagtggcg aaattatgat tgatataaat cacgcctaag ctttgcacat
    12601 cgtccatatt tctggcctgg tggttattaa tttcaatggc tgcccatgta tttgcactta
    12661 gcaaaagcac agccagaagg gctaaaactc gactgaacat agatacctcc tcgacggctg
    12721 actttgtgtg ctctcctctg tgatgatctt ctgatttaat tttaatcaat gataaagaag
    12781 ttgatggtga ccatttctga tgcagttgtt caaaaaaaca ctgtgacgga atgtgatgaa
    12841 cttcaaatca gcggtttaga ggttatttgc gaaaggggag atttatttct gctccgccct
    12901 tgagtttagc ggggtatgtc agtactataa cgcgtcattt ttcagccgac ctttaacacg
    12961 ttccttgcct ccccgggatt cggctgaccc agacaggagg ctgaataatc cgtaaggagc
    13021 aattcgatgc gtcattacga aatcgttttt atggtccatc ctgaccagag cgaacaggtt
    13081 ccgggcatga tcgagcgcta cactgctgcc atcactggtg cagaaggcaa gatccaccgt
    13141 ctggaagact ggggccgccg tcagctggct tacccgatca acaaactgca caaagcacac
    13201 tacgttctga tgaacattga agctccgcag gaagtgatcg atgagctgga aactaccttc
    13261 cgcttcaacg atgccgttat ccgcagcatg gttatgcgta ccaagcacgc tgttaccgaa
    13321 gcatctccga tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga tttcgcaaac
    13381 gaaaccgctg atgatgctga agctggggat tctgaagagt aatttctgat gaccaaccgt
    13441 ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc atcaggaatt
    13501 cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg ctttcaccgg
    13561 caggcatggt gtcaaatgcc cgttattgtt agcggacacg aaaaccaggc cattactcac
    13621 agtataacgg tcggcagtcg cataaccgtt caggggttca tttcatgcca caaggcaaag
    13681 aacggactga gcaaaatggt tttgcacgcc gagcagattg aattgataga ttctggagac
    13741 tagccatatg gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg cggaaggcgt
    13801 tcaagagatc gactataaag atatcgctac gctgaaaaac tacatcaccg aaagcggtaa
    13861 gattgtccca agccgtatca ccggtacccg tgcaaaatac cagcgtcagc tggctcgcgc
    13921 tatcaaacgc gctcgctacc tgtccctgct gccgtacact gatcgccatc agtaatcggt
    13981 cacggtccat taatacgact ttgagaggat aaggtaatgc aagttattct gcttgataaa
    14041 gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta aagcgggcta tgctcgtaac
    14101 ttcctggtac cgcagggtaa agctgttcca gctaccaaga aaaacattga attcttcgaa
    14161 gcacgtcgcg ctgaactgga agctaaactg gctgaagttc tggcagctgc taatgctcgc
    14221 gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg cgacgaaggt
    14281 aaactgttcg gttccatcgg tactcgcgac atcgctgacg ctgtaactgc agctggcgtt
    14341 gaagtggcta agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac tggcgaacac
    14401 gaagtgagct tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa cgtagtagct
    14461 gaataattcg ttattcaacg agacgtaaaa acgccgacca ttggtcggcg ttttgctttc
    14521 tatttttcgt caggcattaa tttcgcaagt agatccattc accattctct tgtgtccata
    14581 agtcaggatg gtttgctggc gttagatgca tacgatcact ccttatgtct cggtcctgcg
    14641 gcactcgctc gaaacctaaa cccgagtaga ttgcggctga atttgataag gaacgtagac
    14701 caattttcaa atcatggggt aaatcagaat acgcgtttag caatgcagca atgatgaatc
    14761 cgagggcatt agaaacgcct gtaacagtgc gaagttggtc gatataaaga tcgattgttg
    14821 gtagttcaga tgaccagcaa ttaggcattt gggcaggaag ggttacaacg tatcctatcg
    14881 tttggccggt atagtctgga gtactataac caggtcccaa aacaacctcg tgtattgtgt
    14941 atttactgtt gggttcgaag gattcgacat cttgcaataa atatttcagt tctgcggtta
    15001 acctcgcacc cgcgtaagct ttattgtatt tctgggcgtg aatattatct aatgtagctt
    15061 catggttcca tccaaaagaa ttatcaccat tcattgt
//
LOCUS       DAJTXY010000095        14904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTXY010000095 DAJTXY010000000
VERSION     DAJTXY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.095"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..306
                     /locus_tag="OO566_002997"
     CDS             <1..306
                     /locus_tag="OO566_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCR6606208.1"
                     /translation="SDDEWWRALLQSRNDHLRQTALRNAHTPASLLTTLTEPQDRSLA
                     INNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRKIRSEARHRLEEKQ"
     gene            303..758
                     /locus_tag="OO566_002998"
     CDS             303..758
                     /locus_tag="OO566_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606209.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(836..1747)
                     /gene="rdgC"
                     /locus_tag="OO566_002999"
     CDS             complement(836..1747)
                     /gene="rdgC"
                     /locus_tag="OO566_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6606210.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1872..2780
                     /gene="mak"
                     /locus_tag="OO566_003000"
     CDS             1872..2780
                     /gene="mak"
                     /locus_tag="OO566_003000"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6606211.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2923..4104)
                     /gene="araJ"
                     /locus_tag="OO566_003001"
     CDS             complement(2923..4104)
                     /gene="araJ"
                     /locus_tag="OO566_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6606212.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(4230..7373)
                     /gene="sbcC"
                     /locus_tag="OO566_003002"
     CDS             complement(4230..7373)
                     /gene="sbcC"
                     /locus_tag="OO566_003002"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6606213.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7370..8572)
                     /gene="sbcD"
                     /locus_tag="OO566_003003"
     CDS             complement(7370..8572)
                     /gene="sbcD"
                     /locus_tag="OO566_003003"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6606214.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8762..9451
                     /gene="phoB"
                     /locus_tag="OO566_003004"
     CDS             8762..9451
                     /gene="phoB"
                     /locus_tag="OO566_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6606215.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9509..10804
                     /gene="phoR"
                     /locus_tag="OO566_003005"
     CDS             9509..10804
                     /gene="phoR"
                     /locus_tag="OO566_003005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6606216.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            11211..12530
                     /gene="brnQ"
                     /locus_tag="OO566_003006"
     CDS             11211..12530
                     /gene="brnQ"
                     /locus_tag="OO566_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6606217.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12606..13979
                     /gene="proY"
                     /locus_tag="OO566_003007"
     CDS             12606..13979
                     /gene="proY"
                     /locus_tag="OO566_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6606218.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            14135..>14904
                     /locus_tag="OO566_003008"
     CDS             14135..>14904
                     /locus_tag="OO566_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HCR6606219.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDS"
BASE COUNT         3302 a         3794 c         4013 g         3795 t
ORIGIN      
        1 tcagatgatg aatggtggcg tgcattgctg caaagtcgca atgatcattt gcgccaaacg
       61 gcattacgca acgcacatac cccggcgtcg ttgttaacga cattgactga gcctcaggat
      121 cggtcgctgg ctattaataa tccacagctg gctgccgatg tgaaaacggc gtggttaaaa
      181 gaggatccat cattactctt atttgtcgaa caacccgatc tttcgcagtt acgtgattta
      241 gtgaaaaccg gggcaacgcg gaaaattcgc agtgaagcgc gtcaccggct tgaggaaaaa
      301 caatgacaca acgtccatgg agcaagttac agcgggagat ctatgactta ctgacgccaa
      361 cgatcaacct gcaaattcac tgtacccgtt atcccatgcg cagccaaaat ggtggaagta
      421 ccgatttacc gcgttattgg atcacgttgg ataaaaacgt gatctgggat tacccgaaag
      481 actttattgc aggtaacgct ggagtgcgta attttcacgg tgaaacttgc tggtatcctt
      541 acctgacgga tatttgttca atttctgatt tattgcgtga atatattgat accccaaaag
      601 cggagctgtt aacaaaacaa tttaccagcg ataaatgggg gttagtgaat attctgcgtg
      661 ctgctgacag gcgaataggg atgaggcgat tagatcagtt acggcgtaaa acgcataata
      721 ttgctgcgct aaaaattatt gctcgccgta gcgaataatt atatgcctgg tgtggcttcg
      781 tacgccggat aagacgcggc aggcgtcgca tccggcatta aaggaaaatc agcaattaac
      841 gttgtgcttc gccacctaat ccttcaatca ggttttgaat taacgctgcc agttcaccag
      901 tcatcaggat gaaatcggca tcaaaacgct gggcgaaatc ttcacggtcg atatcttcgt
      961 tttgatcgtg cagctcgtcg cagaacttaa gacgcttgag cgaaccatcg tcgcacatca
     1021 caaactgaat gcgctgctgc cagtcgagcg ccagtttagt caccactttt ccggcttcaa
     1081 tgtgattggt gatctcttcg ctggtcagat cttgtttctt cgcgcggatc acgccgccat
     1141 cttccagcaa cgatttcagc tcggcttcat caagcagctg gaagccctgt gccgcactac
     1201 cggagcgaac ccattcggtc agcgtcagtt caatcgggtt ttccatgctc aatggtacaa
     1261 ccggtaacga ccccaggctt ttacgcagta atgccagcgt atcttccgct tttttggcac
     1321 tggcgcagtc caccataatc aaaccgttaa ccgtgtcgag ccacatcatt gtctggctaa
     1381 aacggctgaa agcacgcggc agcagagagt gcagcacttc gtctttcagc gaatcttttt
     1441 cggttttctt cagcttacgc gcctgttccg cttccagttt ggcgattttc gcttccagcg
     1501 cctgtttaat caccggagac gggaggattt tttcttcttt gcgcgcgcag ataacaattt
     1561 gaccattggc aacgtgcgtt aacgcatcgc tgtgcgatcc catcggagga acccagccca
     1621 tcttcgccat gtcctggctg ccgcatgggg taaatgccat cgaggctagc tgtttttcca
     1681 tctcttctgc acgcagcgaa atctcgcggc taagacggta aaccattaaa tttttgaacc
     1741 acagcatgat aatttccacg gccttgtcgt taaatttagc gggcatgata acgaattgtc
     1801 ggcggccttg cattgccaat ccggttgtcc gtctctacgc tattgatatt gaaaaaaata
     1861 aggagagtac agtgcgtata ggtatcgatt taggcggcac caaaactgaa gtgattgcac
     1921 tgggcgatgc aggggagcag ttgtaccgcc atcgtctgcc cacgccgcgt gatgattacc
     1981 ggcagactat tgaaacgatc gccacgttgg ttgatatggc ggagcaggcg acagggcagc
     2041 gcggaacggt aggtatgggc attccaggtt cgatttcgcc ctacaccggt gtggtgaaga
     2101 atgccaattc aacctggctc aacggtcagc cattcgataa agacttaagc gcgaggttgc
     2161 agcgggaagt gcggctggca aatgatgcca actgtctggc ggtttcagaa gcagtagatg
     2221 gcgcggcagc gggagcgcag acggtatttg ccgtgattat cggcacggga tgcggcgcgg
     2281 gcgtggcatt caatgggcgg acgcatatcg gcggcaatgg cacggcaggt gagtggggac
     2341 acaatccgct accgtggatg gacgaagacg aactgcgtta tcgcgaggaa gtcccttgtt
     2401 attgcggtaa acaaggttgt attgaaacct ttatttcggg cacgggattc gcgacggatt
     2461 atcgtcgttt gagcggacat gcgctgaaag gcagtgaaat tatccgcctg gttgaagaaa
     2521 gcgatccggt agcggaactg gcattgcgtc gctacgagct gcggctggca aaatcgctgg
     2581 cacatgtcgt gaatattctc gatccggatg tgattgtcct ggggggcggg atgagcaatg
     2641 tagaccgttt atatcaaacg gttgggcagt tgattaaaca atttgtcttc ggcggcgaat
     2701 gtgaaacgcc ggtgagtaag gcgaagcacg gtgattccag cggcgtacgc ggcgctgcgt
     2761 ggttatggcc acaagagtaa aaaacgtagg caattggcgc atcatgcctg atgcgacgct
     2821 tgccgcgtct tatcaggcct acaaaatgtg ccagaaccgc gtagggcgga taaggcgttc
     2881 acgccgcatc cggcaataag caacgcgcac cgtgacagta atctacccca gtggtttcgc
     2941 cagcaccgga ctatccgccg cttgctggcg cttatagcga ccatacagca gcaacgatga
     3001 catcgcagca aacgaaaaca gggcggcagg cagtgccacg taattatatg ccagccccag
     3061 cgtcagcatc atacctccgc aatatgcgcc gacggcgcta ccgaggttaa acgctatttg
     3121 cccacctgcg gcacctaata actctccgcc tttggcattt tgcagtaaca atatttgcag
     3181 aggtgctgaa agggcaaata atcccgcgca acaaataaaa gcaaaaataa gcgacgttgt
     3241 tttcatgccg ccgcagaaaa agagcatcag cagtgccagt acaatgataa agtcagtcac
     3301 tgctgcaatg cgcagtggtg aataacgtcc tgaaatcctg ccacttagca tatttcccag
     3361 caccatccct agcccaacta acatcataat aaaggtcatc gccgtttccg aaaaaccgga
     3421 aataaacatc atgtatggct ttacgtagct gaaccaggca aacacacctg cgttgccaaa
     3481 catcgtggcg gcgaaaatta accacggggc cgggctgcgc aaaaagtgaa attgttcgcg
     3541 cagatttcct ttcgcctcgt cgcgaatatc tggcacccaa aaatagaccg atgccatcac
     3601 cgcaatatta aaaacagcga tcaataaaaa ggtgtaacgc cagcattcct gacttaaata
     3661 cgttcccagc ggaatgccca gcaaattggc gactgtcatc ccggaaacca tccccgccac
     3721 ggcggcggtg acttttccgg gtttgataat ttttgataac acgatcgctc cgacgccaaa
     3781 aaatgcgcca tgcggaaagc cggataccag ccgaccaatg gcgagcatca ggtaagacga
     3841 agagagcgtg aacatggcgt tgccaatgac gcacaacgcc accagaaaca acaagatatg
     3901 tttgagtgag tagcggctgg aaaagagtgc gatgattggc gcaccgacca ccaccaccag
     3961 tgcataatac gagatcatat gcccggcagc aggaatcgaa attcctacgt tatgcgccag
     4021 ctccgtgagc acgctcataa tgccaaattc ggctatcccc aaaccaaacg tgcccagagc
     4081 caaagataaa atgacttttt tcataccacc atcacaacca ccagaacggc taccaatcac
     4141 cgtagccaat aaagtgatag cttgcaggat aacgaccagc aaggccaaga gataattcgc
     4201 ccctctgtat tcattatcct gctgaatagt tatttcactg caaacgtact ttccagtttg
     4261 ctgtagccca ggccgttgat ctttttcact ttgatctgca ccggaatacg ctctttcatc
     4321 gcttctacgt ggctaatcac accgatggtt ttgccactgg cgttcagggc atccagcgca
     4381 tcaagggcgg tatccagcgt ttcgctatcc agcgtgccaa aaccttcatc aaggaacagc
     4441 gagtcaatac gtgttttatg gctgaccaga tccgaaagcg ccagcgccag cgccagacta
     4501 acgaggaaac tttcgccgcc ggaaagggta cgggtatcgc gtaccgcatc tgcctgccag
     4561 gtatcaacaa cctcgacttc cagcgcctcg ctggctttgc gctgtaacag atagcgcccg
     4621 tgcagccggg taagttgctg attagcgaga tggactaaat tatccagcgt cagcccctgg
     4681 gcaaacttgc ggaatttatc gccctctttg gaacctatta gcgaattcag atatccccag
     4741 tcctcaacct gctgcgtcat ttgagcaatt tgctgcatta aggtttgttg ttgctgacgg
     4801 ttatctgcat cctgcttcag ctgctggcga atctcgcctt gactcgtggt gttttcacgc
     4861 aacttttggt gagtttgcgc taactcttgc tgaatctgct ccaccgtcac agtgagagcc
     4921 aacccgtcag gtcggtgttg ttgatgctgt gccagcgttt ctgctgtctg agtgaccagc
     4981 gtttgcgcct gacggcgctg gttttccaga ttctgcttga gctgttccag ctgcgttagt
     5041 gtttgttcat ccattagcgc cgcaaggaac gcctgctgat cgtcaaagac gctggcctgt
     5101 agcgtggtgt caaactgcgc ttgggctttt tgcagacttt gcgccgccag aacatcctgt
     5161 tgctgtaacg tctgctgctg gctgtgtaat gcgagacatt gttcatgaac ctgccgccag
     5221 ttatccagcg ccacagtttc ttcgctgtgc gggagatcat cactttgcgg caacgtttcc
     5281 agaatcggcg tcagctgctg aatacggttt tgcagcgcgg ttaattcgtt ctggcgttgc
     5341 tgccagctct gcgcttcttg ctgacgtgtc gccaaccagc tctcttcttc atcttcctgt
     5401 ggcaatgtca gtgcataacc cgccaatgcc gttaagagct gctgctgacg ttgttcaatt
     5461 tgctgttgat actggataat ttgctgatta tgcgcggcaa tctgcccttg taattcatgt
     5521 cgttggctga gtaaccgcag ctggcgttcg tgctcatctt gtgcatccag ccacggttga
     5581 atatcgtcct gtggctgcaa ggtgatattg aggctggccg tgacggcttg ccattgttga
     5641 gtaagtgctt gctcatcttg tcggaggctt tgcgcttcgt tttcatcgcg ctgaagctgc
     5701 ttcgttaatg catccagttg cccgcgcagc gccgcacctt cttcgccgag ctttttaact
     5761 tcgttttcca gcgccagtaa tcgagactga ttaacaccag gctccagcgc ctgatacgcc
     5821 tcgaccgccg ggtggctggt ggaaccacaa agtggacaag gctgacccgc ctgtaactgt
     5881 gcacgctggg cttccagcgt tttgatgcgc gcttcctgct cgcaaatggt tttcacatcg
     5941 gcaagttgct gcgtcttttc tttataacgc tggcgcattt cgttaagtgc gacattacgt
     6001 tgcgtctgtt ccagagtgac attctggata gtgaccatta actgcgccag acgtttttgt
     6061 tggggaacaa tctgcccatg cagcgcgacc aggcgctgac gcagtgggcg ttgctcggca
     6121 tgttgcgcca gggcggtagc aacttcatcg gcggttaacg tcaacgtgat cgccgcaagc
     6181 gcattaagtt tttgctcagc atgggttaac tgttgctgcc attgccgcag atgctcgcga
     6241 tcgctggttt gttgggagaa ctgcgcacgc caacccgcca gttcgttgtt ccactgacgg
     6301 aagcggtcgt gttcctgtaa ccaggtattc aggctttgtt gctgctgctg taattctgct
     6361 gactgcttcg ccgcgtggtg gcgaatgctc gcgcgaagcg ccattgtgct ctgtaagcga
     6421 gtatttactt cttcaatctg ctggcgaata tgcgccagcg ccgcgctgtg ttctgcgatg
     6481 cgttcccagt gtggacgaag atttcgtgcc ggttgtgcca gactaagcgc cgccagttga
     6541 ggttgcgctt tttcttcttc ggctaacgcc tgttgcaagg cctgctgacg gcggctggct
     6601 tcttgctgca attcgtcctg acgcgttaac cagtttagcg attgttgttc ttgctgctgc
     6661 gcggtaatta actgtttttc ttcgtcagta agtacctgca aactcgctgt cagcgattgc
     6721 acttgttccg gcgtgagcaa cgtgacgccg ctggcctgcg cttgcagctt ctccagctca
     6781 gtacgggccg atttgtgctg ctcaaaaacc atcgccgaga tttgcccgta gatttcagtg
     6841 ccggttaact cctcgagcaa ttccgcgcgt tctttgggtt tggcattcag gaaggcagca
     6901 aattgcccct gcgaaagcag catcgaacgg gtgaagcgcc cgtaatccag cccggttaac
     6961 gtcgctgtca gttccagctt atctttcact ttgtcggcga gaattttgcc gtcggcgcag
     7021 cgcgccagct ctacgcgagg cacctgcaaa ttaccgtccg gctggttacg cgcccgattc
     7081 tggctccaga acgcgcgata cgcttcacct ttcacttcaa actccacctc cgccagacat
     7141 tcagcggtat cgcgggtcat gagatcattt tgcgattgtg aaacgttaga gagacgcgga
     7201 gtttcgtgat acagcgccag acaaatggcg tccagcaggg tggttttccc cgcaccggtt
     7261 gggccggtaa tggcaaacag cccgttgctg gcgaacggct cgcgggtgaa atcaatcttc
     7321 cattcgcctt ttaatgagtt caggtttttc aggcgcaggc tgagaatttt catgcttcgt
     7381 gttctccggc gagggtatgc aacgtcgtgg tgaaaagatg ctgcagacgt tgctgctgcg
     7441 attcatccag ttcttccagt gccagacggc gattgaacac ctcttcgacg ctgagttcgc
     7501 tgagggtttc acgctgttgg ctggctaaca cacgctcgcg ctgttcacga ctccgacgta
     7561 ccagcaatac ttcgacaggc aatgattcgg ttaatgcctg gattttgcgc tgaatatcat
     7621 gcagatactc atcagtagtg atttcgatat ccagccagac aggtggctcc tgcgatacat
     7681 cgcgccactg ttccagctgt gcggtaatcg acgccagatc gcctttcagc accgccatgg
     7741 gttgagttac cggtacgttc aggttttcca cgctctctaa tttgccgttt gaaaatgtca
     7801 ccagatggac atatttactc ttaccgcatt catcaaaact cagtggaatg ggggaaccgc
     7861 aatagcgaac atgttccatg ccgccaataa tctgtgcgcg gtgaatatgc ccgagcgcga
     7921 tgtagtcggc tggtggaaag ttttgtgccg gaaacgcgtc cagcgtgcca atataaatgt
     7981 cacgcacggc gtcactttta ctggcaccca cggtcgttaa atgtcccgtg gcgatgatgg
     8041 gcagaggctg atcgccgcgc agtttgcagg catcggcata gtgttgttgg taataatcgg
     8101 taatcgctgc cagtaaatgc tgctgttttt caataccgtt aagccccgcc tggctggaaa
     8161 taatgtcacg cgggcgtaaa aacggaatgg ggcacagcac tgcgcctggc gtcccgtcgc
     8221 gacgaggcaa gatttgcggc gcatgtccgg cgctggcgac cacggttgta ttgaggaacg
     8281 ccatgatatc gcgcgattca ttcagcgtgg cgaccgagtc atggtttcct gccagtacca
     8341 ccagatgaca gccagtttgc tgtaaattga caacaaaacg gttgtataac gtgcgggcgt
     8401 aactgggcgg cgagccggta tcgaaaacat caccagcaac aataatcgca tccacctgat
     8461 gggtttgtgc tgtctccagc agccagtcaa gaaaagcctg atgttcagct tcgcggcttt
     8521 tactgtagaa gttctggccg agatgccagt ctgaggtgtg aaggatgcgc ataacggttc
     8581 cctggcgaaa aagcatggtc gcgattatac ccaaacagat gtgccatttg cttttttctg
     8641 cgccacggaa atcaataacc tgaagatatg tgcgacgagc ttttcataaa tctgtcataa
     8701 atctgacgca taatgacgtc gcattaatga tcgcaaccta tttattacaa cagggcaaat
     8761 catggcgaga cgtattctgg tcgtagaaga tgaagctcca attcgcgaaa tggtctgctt
     8821 cgtgctcgaa caaaatggct ttcagccggt cgaagcggaa gattatgaca gtgctgtgaa
     8881 tcaactgaat gaaccctggc cggatttaat tctccttgac tggatgttac ttggcggctc
     8941 cggtatccag ttcatcaaac acctcaagcg cgagtcgatg acccgggata ttccagtggt
     9001 gatgttgacc gccagagggg aagaagaaga tcgcgtgcgc ggccttgaaa ccggcgcgga
     9061 tgactatatc accaagccat tttcgccgaa ggagctggtg gcgcgaatca aagcggtaat
     9121 gcgccgtatt tcgccaatgg cggtggaaga ggtgattgag atgcagggat taagtctcga
     9181 cccgacatct caccgagtga tggcgggcga agagccgctg gagatggggc cgacagaatt
     9241 taaactgctg cactttttta tgacgcatcc tgagcgcgtg tacagccgcg agcagctgtt
     9301 aaaccacgtc tggggaacta acgtgtatgt ggaagaccgc acggtcgatg tccacattcg
     9361 tcgcctgcgt aaagcactgg agcccggcgg gcatgaccgc atggtgcaga ccgtgcgcgg
     9421 tacaggatat cgtttttcaa cccgctttta acgccttgct catcggacgc agagcagggc
     9481 ttatgatttc ttaactggag tatcttacgt gctggaacgg ctgtcgtgga aaaggctggt
     9541 gctggagctg ctactttgct gcctcccggc tttcatcctg ggtgcatttt ttggttacct
     9601 gccctggttt ttgctggcat cggtaacagg actgcttatc tggcatttct ggaatttatt
     9661 gcgcctttca tggtggctgt gggtggatcg cagtatgacc ccgccaccgg ggcgtggtag
     9721 ctgggaaccg ctactatacg gcttacacca gatgcagctg cgaaataaaa aacgccgccg
     9781 tgaactgggc aatctgatta aacgctttcg tagcggcgcg gagtcgctgc ccgacgcggt
     9841 ggtgctgacc acggaagagg gcggtatttt ctggtgtaac ggtctggcgc aacaaattct
     9901 tggtttgcgc tggccggaag ataacgggca gaacatcctt aacctactgc gttacccgga
     9961 gtttacgcaa tatctgaaaa cgcgtgattt ttctcgcccg ctcaatctgg tgctcaacac
    10021 cgggcggcat ctggaaattc gcgtcatgcc ttatacccac aaacagttgt tgatggtggc
    10081 gcgtgatgtc acgcaaatgc atcaactgga aggggcgcgg cgtaactttt ttgccaacgt
    10141 gagccatgag ttacgtacgc cattgaccgt gttacagggt tacctggaga tgatggatga
    10201 gcagccgctg gaaggcgcgg tacgcgaaaa agcgttgcac accatgcgcg agcagaccca
    10261 gcggatggaa gggctggtga agcagttgct aacgctgtcg aaaattgaag cctcgccgac
    10321 gcaattgctc aatgaaaagg ttgatgtgcc gatgatgctg cgcgttgttg agcgcgaggc
    10381 tcagactctg agtcagaaaa aacagacatt tacctttgag atagataacg gcctcaaggt
    10441 gtctggcaac gaagatcagc tacgcagtgc gatttcgaac ctggtctata acgccgtgaa
    10501 tcatacgccg gaaggcacgc atatcaccgt acgctggcag cgagtgccgc acggtgccga
    10561 atttagcgtt gaagataacg gaccgggcat tgcaccggag catattccgc gcctgaccga
    10621 gcgtttttat cgcgttgata aagcgcgttc ccggcaaacc ggcggtagcg gattagggtt
    10681 agcgatcgtg aaacatgcgg tgaatcatca cgaaagtcgc ctgaatattg agagtacagt
    10741 aggaaaagga acacgtttca gttttgttat cccggaacgt ttaattgcca aaaacagcga
    10801 ttaatccgcc tttgtcatct tttattgcca taagccagcc gatgctggct tattttcttt
    10861 gcagtcaaaa tacgggcgtt agattttaca acggttggtg attttttgtt cgcatgatta
    10921 gccatgtctt tttcacggaa atagtgtttt atactggttg gtgatttctt atcgctatat
    10981 acctctggtt tttagatccc tccttgcttt aaaacgttat aagcgtttaa attgcgcttc
    11041 aggtgctgtc atactgactg cattaacgcg gtaaatcgaa aaactattct tcgccgcgcc
    11101 tggttgggag tattttccgc taaaattgtt taaatatacc gctgtatcat ccccagggat
    11161 tggcacaaaa atttaacgtt acaacaccac atccacaggc agtatgattt atgacccatc
    11221 aattaagatc gcgcgatatc atcgctctgg gctttatgac atttgcgttg ttcgtcggcg
    11281 caggtaacat tattttccct ccaatggtcg gcttacaggc aggcgaacac gtctggactg
    11341 cggcattcgg cttcctcatt actgccgttg gcctgccggt attaacggta gtggcgctgg
    11401 caaaagttgg cggcggtgtt gacagcctca gcacgccaat cggtaaagtc gctggcgtac
    11461 tgctggcaac ggtttgttac ctggcggtgg ggccgctttt cgctacgccg cgtacagcta
    11521 ccgtttcctt tgaagtgggg attgcgccgc tgacgggtga ttccgcgctg ccgctgttta
    11581 tctacagcct ggtctatttc gctatcgtta ttctggtttc gctctatccg ggcaagctgc
    11641 tggataccgt gggcaacttc cttgcgccgc tgaaaattat cgcgctggtc atcctgtctg
    11701 ttgtcgcgat tgtctggccg gcgggttcta tcagcacggc gactgaggct tatcaaaacg
    11761 ctgcattttc taacggcttc gttaacggct atctgaccat ggatacgctg ggcgcaatgg
    11821 tgtttggtat cgttattgtt aacgcggcgc gttctcgtgg cgttaccgaa gcgcgtctgc
    11881 tgacccgtta taccgtctgg gctggcctga tggcgggtgt tggtctgact ttgctgtacc
    11941 tggcgctgtt ctgtctgggt tcagacagcg cgtcgctggt cgatcagtct gcaaacggcg
    12001 ctgctattct gcatgcttac gttcagcaca cctttggcgg cggcggtagc ttcctgctgg
    12061 cggcgttaat cttcatcgcc tgtctggtca cggcggttgg cctgacctgt gcttgtgcag
    12121 aattctttgc ccagtacgta ccgctctctt atcgtacgct ggtgtttatc ctcggcggct
    12181 tctcgatggt ggtgtctaac ctcggcttaa gccagctgat ccagatctcc gtaccggtgc
    12241 tgaccgctat ttatccgccg tgtatcgcac tggttgtatt aagttttaca cgctcatggt
    12301 ggcataattc gtcccgcgtg attgctccgc cgatgtttat cagcctgctt tttggtattc
    12361 tcgacgggat caaagcatct gcattcagcg atatcttacc gtcctgggcg cagcgtttac
    12421 cgctggccga acaaggtctg gcgtggttaa tgccaacagt ggtgatggtg gttctggcca
    12481 ttatctggga tcgcgcggca ggtcgtcagg tgacctccag cgctcactaa atcactgaac
    12541 atttgtttta accacggggc tgcgatgccc cgtggttttt tattgtgttg atgggttagg
    12601 aattgatgga aagtaagaat aagctaaagc gtgggctaag tacccgccac atacgcttta
    12661 tggcactggg ttcagcaatt ggcaccgggc tgttttacgg ttcggcagac gccatcaaaa
    12721 tggccggtcc gagcgtgttg ttggcctata ttatcggtgg tatcgcggcg tatatcatta
    12781 tgcgtgcgct gggggaaatg tcggtacata acccggccgc cagctctttc tcgcgttatg
    12841 cgcaggaaaa ccttggcccg ctggcaggtt acattaccgg atggacctac tgctttgaaa
    12901 tccttattgt cgccatcgcc gatgtgaccg cttttggtat ctatatgggg gtcttgttcc
    12961 cgacggtgcc gcactggatt tgggtactga gcgtggtgct gatcatttgc gccgtaaacc
    13021 tgatgagcgt gaaggtcttc ggtgagctgg aattctggtt ctcgttcttt aaagtcgcca
    13081 ccatcatcat catgattgtc gccggtttcg gcatcatcat ctgggggatt ggcaacggcg
    13141 ggcaaccgac cggtattcat aacctgtgga gcaacggcgg cttcttcagt aacggctggc
    13201 ttggcatggt aatgtcgttg caaatggtga tgtttgctta cggtgggatc gaaattatcg
    13261 ggattaccgc cggtgaagcg aaagatcctg agaaatcgat tccgcgtgcg attaactccg
    13321 tgccgatgcg tattctggtg ttctacgtcg gtacgctgtt cgtcattatg tctatctacc
    13381 cgtggaatca ggttggcact gccggtagcc cgttcgtgct gacgttccag catatgggca
    13441 ttacctttgc cgccagcatt cttaactttg ttgtgctgac cgcttcgctg tcggcaatta
    13501 acagtgacgt atttggcgta ggccgtatgc tccacggtat ggcagagcag ggcagcgcac
    13561 cgaaaatttt cagcaaaacg tcgcgtcgcg gtattccgtg ggttacggtg ctggtgatga
    13621 ctaccgcgct gctgtttgcg gtgtatctga actacatcat gccggaaaac gtcttcctgg
    13681 tgatcgcttc gctggcaacc ttcgccacgg tatgggtgtg gattatgatc ctgctgtcgc
    13741 aaatcgcctt ccgtcgccgt ttgccgccag aagaagttaa ggcgctgaaa tttaaagtgc
    13801 cgggtggggt agcaacgacc atcggcgggc tgattttcct gctctttatt atcgggttga
    13861 ttggttatca cccggatacg cgtatctcgc tgtatgtcgg tttcgcgtgg attgttgtgc
    13921 tgttgattgg ctggatgttt aaacgccgcc acgatcgtca gctggctgaa aaccaataat
    13981 acttgcgttc tgtaagccgg ttcaggagga aatcctgatc cggctattcc gaaagttatc
    14041 cgcccccgtc ctcctccccc aaatatcctt cagatgatga gtgatcctgc attaggctat
    14101 ggcaaggtga tcagattttc atcacagggg aattatgatg ttaaatgcat ggcacctgcc
    14161 ggtgccccca tttgttaaac aaagcaaaga tcaactgctc attacactgt ggctgacggg
    14221 cgaagaccca ccgcagcgca ttatgctgcg tacagaacac gataacgaag aaatgtcagt
    14281 accaatgcat aagcagcgca gtcagccgca gccaggcgtc accgcatggc gtgcggcgat
    14341 tgatctctcc agcggacaac cccggcggcg ttacagtttc aaactgctgt ggcacgatcg
    14401 ccagcgttgg tttacaccgc agggcttcag ccgaatgccg ccggcacgac tggagcagtt
    14461 tgccgtcgat gtaccggata tcggcccaca atgggctgcg gatcagattt tttatcagat
    14521 cttccctgat cgttttgcgc gtagtcttcc tcgtgaagct gaacaggatc atgtctatta
    14581 ccatcatgca gccggacaag agatcatctt gcgtgactgg gatgaaccgg tcacggcgca
    14641 ggcgggcgga tcaacgttct atggcggcga tctggacggg ataagcgaaa aactgccgta
    14701 tctgaaaaag cttggcgtga cagcgctgta tctcaatccg gtgtttaaag ctcccagcgt
    14761 acataaatac gataccgagg attatcgcca tgtcgatccg cagtttggcg gtgatggggc
    14821 gttgctgcgt ttgcgacaca atacgcagca gctgggaatg cggctggtgc tggacggcgt
    14881 gtttaaccac agtggcgatt ccca
//
LOCUS       DAJTXY010000096        14731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTXY010000096 DAJTXY010000000
VERSION     DAJTXY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.096"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            403..600
                     /locus_tag="OO566_003009"
     CDS             403..600
                     /locus_tag="OO566_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606220.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(550..1743)
                     /locus_tag="OO566_003010"
     CDS             complement(550..1743)
                     /locus_tag="OO566_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6606221.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1879..3603
                     /gene="iucA"
                     /locus_tag="OO566_003011"
     CDS             1879..3603
                     /gene="iucA"
                     /locus_tag="OO566_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6606222.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3604..4551
                     /gene="iucB"
                     /locus_tag="OO566_003012"
     CDS             3604..4551
                     /gene="iucB"
                     /locus_tag="OO566_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6606223.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4551..6293
                     /gene="iucC"
                     /locus_tag="OO566_003013"
     CDS             4551..6293
                     /gene="iucC"
                     /locus_tag="OO566_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6606224.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6290..7606
                     /gene="iucD"
                     /locus_tag="OO566_003014"
     CDS             6290..7606
                     /gene="iucD"
                     /locus_tag="OO566_003014"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6606225.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7612..9807
                     /gene="iutA"
                     /locus_tag="OO566_003015"
     CDS             7612..9807
                     /gene="iutA"
                     /locus_tag="OO566_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6606226.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10358..10501)
                     /locus_tag="OO566_003016"
     CDS             complement(10358..10501)
                     /locus_tag="OO566_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606227.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10751..14638
                     /gene="sat"
                     /locus_tag="OO566_003017"
     CDS             10751..14638
                     /gene="sat"
                     /locus_tag="OO566_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCR6606228.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         3941 a         3523 c         3672 g         3595 t
ORIGIN      
        1 gatgaaagaa acttatttct tcatcctcaa ctcgtcatca gtgagtcagg tgtttgcctc
       61 ggtgtttatg atgactatca gtggttcagg gatgaattaa aaaccagtaa aaatacccga
      121 caagaaatct gcaatgattt acttcataaa aaacatgtca gtgaaaaaga aacctggcgt
      181 tgggttgaag gatataataa agcaacagaa ctggctcgcc aatgccctga tacccatgta
      241 ttgtcaattt ctgatcgtga aggcgatttt tatgacctgt ttgaacgcgc agcgcagact
      301 cccggaataa aagctgactg gcttgtcaga atgaaattta ataacagagc aacgctgaat
      361 attaatggta aacgggatca taggttgttg cacgaaagaa tcatggagat tacaccgcag
      421 caactggttg aattcactat acctgatggg cgggggcagc aagccagaac actgcctgac
      481 atgctttcat cagacgcagg tcctctctca tatcctctga tttactccag taaggtgatg
      541 atcatatctt tatttgatag tacggtaatg ccagataaat acagacacaa tggtaaataa
      601 tatagttgca aataatgctg ccataatcag cgcgattgcg acacctgttt tcgataccag
      661 cggcaccagc aaagcgcaca gtccatagcc aagtgtgtga ctggtagcga gccagcccgc
      721 gcctgcgcca tcagccagct tatcattcag tagaagttga taagcgggtg ttgccagagc
      781 tgctccaaat gacagcactg cacagcccgg gtaaaacaac catagctgtt cggagagcat
      841 gatagccaga ccaccactca tcaacactcc ggcactcagg agcagggcca ccggagtcag
      901 gcgctgcgga cggagtaccc cgaactgcgc gataagcgca gctactgcgg acagacccaa
      961 cagccaggcc acctgttggc taatggcggt ggtatccgta gcgaactggc gagtaagggc
     1021 aggcgaaagt ccaagctgca tcatgctcac cgccgcagcc agtaatattg cgcaaagcag
     1081 ataaggcaga caatcccgct tcagactgac gctcttgcac tccggtgtgg gctgtggtgg
     1141 tgtgccgggc aaccgcagca gcatcagcag cgccagcact ggggctgcca tcagcagccc
     1201 cagtggcgcc agaggatgaa tggccagcat tgctgccgcg cacagcggcc cgaatagcct
     1261 gccgcaactc aggccggagc tgatggttgc cagagcggcc atgcgattcc cttctccagc
     1321 tctctgcaat gcccagacct gacaggctgg caccattgcc gacacggtca gcccgtaggc
     1381 gatccgggcg atgattaata ttcccagtcc caccgctgtt gttatggcgc tggtagccat
     1441 cagcacgctt cccagcccta gcagggtaaa gcttatgcca tatcctccca gagcccacaa
     1501 caccacaggt ttactgccag tacgggaaat ttgctttccc caccacgggg acgatggcag
     1561 aaacagcatt gagccaagca tcagcagggc agcccataca gacagactca gatttgtctg
     1621 tatgaccaga acagggagca caaccagcag accgttctgt ccgataccga gaagcccggc
     1681 actgaacgca agtggccagc aggacagtgg tttttggggc gtatcttcga tccctggtga
     1741 cattataatg tttcaactcc atgtattaat tgtgtttatt tgtaaaatta atttatctga
     1801 caataacatt tattgttgat aatgagaatc attattgaca taattgttat tattttactg
     1861 tgtgggagct gtttgactat gaccctgccc tctgaaaaac cagccacaga tgtggctgcg
     1921 cagtgcttcc tgaatgcact gattcgtgaa accacagact ggaaactgac agaatacccg
     1981 ccagacgaat tgctcatccc gctggatgag cagaaatcgc tccatttcag agtggcttat
     2041 ttctccccaa cccagcatca ccgctttgca tttcctgcac gtctggtcac ggcatcaggc
     2101 agttatcctg tcgactttac cactctctcc cggcttatta ttgataagct acgccatcaa
     2161 ctgtttctgc ccgttcccct ctgcgaaact ttccaccagc gcgtgctgga aagccatgtc
     2221 catacgcaac aggcaattga tgcccgtcat gactgggccg ctctgcgtga aaaagcgttg
     2281 aattttggcg aggctgagca ggcgctgctg acaggacacg ctttccaccc tgcgcctaag
     2341 tctcatgaac cgtttaaccg gcgggaggct gaacgctacc tgcctgatat ggcaccccac
     2401 ttcccactgc ggtggttttc ggtggataaa acgcaaatcg ccggtgaaag tttgcatctc
     2461 aaccttcaac agcggctgac gcgatttgcc gcagagaatg cgcctcagtt actcaacgaa
     2521 ttaagtgaca accaatggct gttcccgttg cacccgtggc agggggaata tcttttgcag
     2581 caggggtggt gccaggcact tgttgctaaa gggctgatta aagacttagg tgaggccggc
     2641 acgtcgtggc tgccgaccac ctcttcccgt tccctctact gtgccaccag ccgcgatatg
     2701 atcaagttct ccctgagcgt acggctgacc aactccatcc gtactctgtc cgtgaaagaa
     2761 gtgaagcgtg ggatgcgcct ggcacgcctg gctcaaaccg acggctggca gatgctacag
     2821 gtccgcttcc cgactttccg ggtaatgcag gaggatggct gggccgggct gctcgatctt
     2881 aacggcaaca tcatgcagga aagtctgttt gccctgcgtg aaaatctgct ggtggatcag
     2941 ccgaaaagcc agactaacgt actggtctcc ctgactcagg ccgcacctga tggcggtgat
     3001 tcgctgctgg tttcggcggt aaaacgcctg agcgatcgcc tcggtatcac tgtgcaacag
     3061 gccgcccatg catgggtcga tgcatactgt cagcaagtgc taaagccgct gtttacggct
     3121 gaagcggatt acggcctggt gctgctggcg catcagcaaa atattcttgt ccagatgctt
     3181 ggggatctgc cggtcggatt tatttaccgt gactgtcagg gcagcgcttt tatgcctcac
     3241 gcgacagact ggctcgattc tattggcgag gcgcaggcgg aaaatatttt cacccatgag
     3301 cagttgctgc gctatttccc ttattacctg ctggttaact ccacttttgc tgtgaccgct
     3361 gcgctggggg ctgccgggct ggacagcgaa tcgaatctga tggctcgtgt acgagcatcg
     3421 ctggctgaag tgcgtgatca ggtgactcat aaaacctgtc tcaactacgt gctggaaagt
     3481 ccgtactgga acgtaaaagg taactttttc tgttatctga acgatcataa cgagaacacc
     3541 attgttgacc cttcggtgat ctatttcgat ttcgctaacc cgctgcaggc tcaggaggtc
     3601 tgaatgtctg aggcaaacat tattcacagc agatatggac tgcgctgtga aaaactcgac
     3661 aagcccctga atcttggctg gggactggac aatagcgcgg tgttgcactg tcccggggag
     3721 ctgccgacag ggtggctgtg cgatgcgctg gatcagatat ttatcgccgc accacaactg
     3781 tcagcagtgg ctctcccctg ggccgaatgg cgtgaggagc cacaggcgct gacgcttttt
     3841 gggcaggtaa aaagcgacat tatccatcgt actgccttct ggcagttacc gttatggttg
     3901 agttcaccgg caaaccgggc ctccggtgaa atggtttttg atgcagagcg tgagatttat
     3961 ttcccgcagc gcccaccccg tccacagggt gaggtttacc gccgttacga tccacgtatt
     4021 cgcaggatgc tgagtttccg cattgccgat cccgtttctg atgcagaacg tttcacccgc
     4081 tggatgaatg atccgcgcgt tgagtatttc tgggagcaaa gtggctcact ggaggtacag
     4141 accgcctatc tggagcgcca gctcaccggt aaacatgcat tcccgctgat tgggtgcttc
     4201 gacgatcggc cattcagcta tttcgaaatc tactgggcgg cggaagaccg cattggtcgt
     4261 cactattcgt ggcagccctt tgaccgtggc ctgcatctgc tggttggtga acagcaatgg
     4321 cgcggggccc actatgtgca aagctggctg cgcgggctga cacattacct gctgctggat
     4381 gagccccgta cgcagcgcac agtactggag ccacgcaccg ataaccagcg cctgttccgc
     4441 catcttgagc ctgcgggata ccggacaatt aaagagttcg acttcccaca taagcgctcg
     4501 cgcatggtga tggcggatcg tcatcacttc ttcacggagg tcggtctgta atgaatcaca
     4561 aggactggga ttttgttaac cgtcggctgg tggcaaaaat gttgtctgag atggagtatg
     4621 agcaggtttt ccacgcggaa tctcagggcg atgaccacta ctgcattaac ctgccgggag
     4681 cacaatggcg cttcatcgct gaacgtggta tctggggctg gctgtggatt gatgcccaaa
     4741 cgttgcgctg cacggacgaa ccagtactgg cgcagacgct gctgatgcag ctaaagccgg
     4801 tgctgtcaat gagtgatgcc actgttgcag agcatatgca ggatttgtat gccacgctac
     4861 tgggcgacct gcaactgctg aaggcccgtc gcggactgag tgccagtgac ctgattaatc
     4921 ttgatgccga tcgtctgcaa tgcctgctga gcggtcatcc taaattcgtt tttaataaag
     4981 gtcgccgtgg ctggggcaaa gaggcgctgg aacgatatgc gccagagtat accaacacct
     5041 tcagactgca ctggctggcg gtaaaacgtg aacatatgat ctggcgctgc gataacgatc
     5101 tggatattca gcagttgttg actgccgcaa tggatccgca ggagtttaca cgcttcagtc
     5161 aggtctggca ggaaaacggg ctggatcaca actggctgcc gctgccggta catccgtggc
     5221 agtggcagca aaaaatcgcc acagacttca tcgctgattt tgccgaaggc aggatggtgt
     5281 ctctcggcga gtttggcgac cagtggctcg cccagcagtc gctgcgtacc ctgaccaacg
     5341 ccagccgccg gggagggctg gatatcaagc tgccgctgac catctacaat acctcatgct
     5401 accgggggat ccctggcaga tatatcgctg ccggaccact ggcttcacgc tggctgcaac
     5461 aggtttttgc gaccgacgcc accctggtgc aaagcggcgc agtgatcctt ggtgaaccgg
     5521 ctgcaggcta tgtgtcccat gaaggctatg ccgcgcttgc ccgggctccc tatcgctacc
     5581 aggaaatgct tggtgttatc tggcgggaga atccgtgccg ctggctgaaa ccggatgaaa
     5641 gtcctgttct gatggcaaca ctgatggagt gcgacgaaaa caatcagccg ctggcaggcg
     5701 catatatcga ccgctccgga ctggacgctg aaacctggct tacgcaattg ttccgggtgg
     5761 ttgtggttcc gctgtatcac ctgctttgcc gctacggtgt cgcgcttatt gcacacggac
     5821 aaaatataac gctcgccatg aaagaggggg taccacagcg tgttctgctg aaagacttcc
     5881 agggcgatat gcggctggtg aaagaagcgt ttcccgaaat ggactctttg cctcaggagg
     5941 tgcgtgatgt cacatcccgt ctgagtgcgg actacttaat ccatgatttg cagacgggtc
     6001 acttcgtgac agtactgcgt tttatttcgc cactgatggt tcgtcttggc gtacctgaaa
     6061 ggcgatttta tcaactgctg gcagaagtgt tgagtgatta catgaacaaa catccacaaa
     6121 tggcagagcg ttttgcgctt ttctcactct tcaggccaca aatcattcgc gtggtgctga
     6181 acccggtaaa actgacctgg ccggacctgg atggcggcag tcgcatgctg ccgaattacc
     6241 ttgagaatct gcaaaatccg ctgtggctgg taactcagga atatgaatca tgaaaaaaag
     6301 tgtcgatttt attggtgtcg ggacagggcc atttaatctc agcattgctg cattgtcaca
     6361 tcagatcgaa gaactgaact gtctcttctt tgacgaacat cctcattttt cctggcatcc
     6421 gggtatgctg gtaccggatt gtcatatgca gaccgtcttt ctgaaagatc tggtcagtgc
     6481 agttgcacct acaaatccct acagctttgt taactatctg gtgaagcaca aaaagttcta
     6541 tcgcttcctt acaagcaggc tacgtacagt atcccgtgaa gagttttctg actatctccg
     6601 ctgggctgct gaagatatga ataacctgta tttcagtcat accgttgaaa atattgattt
     6661 cgacaaaaaa agtcgattgt ttctggttca gaccagtcgg ggagaatatt ttgcccacaa
     6721 tatctgcctt ggtacaggaa aacaacctta tttaccaccc tgtgtgaagc atgtgacaca
     6781 atcctgtttc catgccagtg aaatgaatct tcgtcggccg gacctgagtg gaaaacggat
     6841 aaccgtggtt ggtggaggac agagtggtgc agacctgttc cttaatgcat tacgcgggga
     6901 atggggagaa gcggcggaaa taaactgggt ctcacggcgt aataatttta acgcactgga
     6961 tgaggctgct tttgctgatg agtattttac acctgaatat atttcaggct tctccggact
     7021 gaaggaagat attcgccatc agttactgga tgagcagaaa atgacatcgg atggcatcac
     7081 tgccgattct ttactgacca tttatcgtga gttgtaccac cgttttgaag ttctgagaaa
     7141 accaagaaat atccgtctgc tacccagtcg ctcggtaaca actctggaaa gtagtggtcc
     7201 gggctggagg ttattgatgg aacatcatct ggatcagggc agagagagcc tggaaagtga
     7261 tgtggtgatt ttcgccacag gttaccgttc tgcattgcca caaatacttc cctcactgat
     7321 gcccttgatc accatgcacg ataagaacac ctttaaagtg cgtgatgact tcactctgga
     7381 atggagtggc ccgaaagaaa ataacatctt tgcggtcaac gccagcatgc aaactcatgg
     7441 catcgccgaa ccccagctca gcctgatggc ctggcgatct gcacgtattc ttaatcgcgt
     7501 actggggcgt gatttattcg atctcagtat gccgcccgca ctgattcagt ggcgcagcgg
     7561 cagccctgct tctttaactc actatacagc aaatattcag gaataacgat gatgcgcaaa
     7621 aagtatatgc cccgggctct tggtccgctg cttcttgtcg tgctgtcacc agctgtcgcc
     7681 cagcaaaacg atgataatga gatcatagtg tctgccagcc gcagcaatcg aactgtagcg
     7741 gagatggcgc aaaccacctg ggttatcgaa aatgccgaac tggagcagca gattcagggc
     7801 ggtaaagagc tgaaagacgc actggctcag ttaatccccg gccttgatgt cagcagccaa
     7861 agtcgaacca actacggtat gaacatgcgt ggccgcccgc tggttgtcct gattgacggt
     7921 gtgcgcctca actcttcacg ttccgacagc cgacaactgg actctgtcga tccttttaat
     7981 atcgaccata ttgaagtgat ctccggcgcg acggccctgt acggtggcgg gagtaccgga
     8041 gggttgatca acatcgtgac caaaaaaggt cagccggaaa ccatgatgga gtttgaggct
     8101 ggcacaaaaa gtggctttaa cagcagtaaa gatcacgatg agcgcattgc cggtgctgtc
     8161 tccggcggaa atgaccatat ctccggacgt ctttccgtgg catatcagaa atttggcggc
     8221 tggtttgacg gtaacggcga tgccaccctg cttgataaca cccagaccgg cctgcagcac
     8281 tccaatcggc tggacatcat gggaaccggt acgctgaaca tcgatgaatc ccggcagctt
     8341 caactgataa cgcagtacta taaaagtcag ggagacgaca attacgggct taatctcggg
     8401 aaaggctttt ccgccatcag cgggagcagc acaccatacg tcagtaaggg gctgaattct
     8461 gaccgcattc ccggcactga gcggcatttg atcagcctgc agtactctga cagtgatttc
     8521 ctgggacagg aactggtcgg tcaggtttac taccgcgatg agtcgttgcg gtactacccg
     8581 ttcccgacgg taaatgcgaa taaacaggcg acggctttct cctcgtcaca gcaggatacc
     8641 gaccagtacg gcatgaaact gactctgaac agccaactga tggacggctg gcaaatcacc
     8701 tgggggctgg atgctgagca tgagcgcttt acctccaacc agatgttctt cgatctggct
     8761 caggcaagtg cttccggagg gctgaacaac cataagattt acaccaccgg gcgctatccg
     8821 tcatatgaca tcaccaatct ggcggccttc ctgcaatcca gctatgacat taatgatatt
     8881 tttaccgtta gcggtggcgt acgctatcag tatactgaga acagggtaga tgatttcatc
     8941 gactacacgc agcaacagaa gattgctgcc gggaaggcga tatctgccga cgccattcct
     9001 ggtggttcgg tagattacga taactttctg ttcaatgctg gtctgctgat gcacatcacc
     9061 gaacgtcagc aggcatggtt caatttttcc cagggggtgg cattgccgga tccggggaaa
     9121 tattatggtc gcggcatcta tggtgcagca gtgaacggcc atcttcccct gacaaagagc
     9181 gtgaacgtca gcgacagtaa gctggaaggc gtgaaagtcg attcttatga actgggctgg
     9241 cgctttatcg gtgacaacct gcggactcaa atcgcggcat attactcgct ttccaataag
     9301 agcgtggaaa ggaataaaga tctgaccatc agtgtgaagg acgacaggcg ccgtatttac
     9361 ggcgtggaag gtgcggtgga ctacctgatc ccggatactg actggagtac cggtgtgaac
     9421 ttcaatgtgc tgaaaaccga gtcgaaagtg aacggtcaat ggcaaaaata tgacgtgaag
     9481 gaatcaagtc catcgaaagc gacagcttac attaactggg cgccggaacc gtggagtctg
     9541 cgtgtacaga gcaccacttc tttcgacgta agcgatgcag agggtaacga tattaatggt
     9601 tacactaccg tcgattttat cagtagttgg cagcttccgg tgggaacact cagcttcagc
     9661 gttgagaacc tcttcgaccg tgactatacc actgtctggg gacagcgtgc acctctgtac
     9721 tacagcccgg gttacggccc tgcttcactg tacgactaca aaggccgggg ccgaaccttt
     9781 ggtctgaact actcagtgct gttctgacgg ggatgctttt gcaacaaggg tatgctgtaa
     9841 acaacatggt ccttggtggc catgttgttt acagccagta taatatacag ccttaacaag
     9901 atattccgtc tccggaatgg gctgttcacc actgaataac attatgtctg ttttatggat
     9961 ctttgactga aatttatcgc atatcctcat ttcattgtcc gtctgaaaac taaccttaca
    10021 agtaagcgtc gtctcagcac cgtctggcag atcctgaaat tcctgagagg atgatctgcg
    10081 cccaatatcc tgagccggaa ctttgctgca atgaaggcca acgaaaaatg ggggagtatg
    10141 ttatagagta cttgctacag ggtaaaaaga ggtacctgtc gctggttctc cgttcagttt
    10201 aaccgggaga ttttctcctg cgcctgtcat aaagtccggt aatggagatt gttaatacga
    10261 tggtgcacga tgcatcgtca gtgcccggcc cggaggatgc cccgtagcta catacggatc
    10321 agggttggca atatcgaatg gcaggctata ggcaaagtta aagccccatg gagtagcaca
    10381 aaatatgccg cacaaaggaa acggtctgaa taacgcagtg atggagaact tcttcggcac
    10441 actgcaaaat cgcatcgtgg tcgatcgcta attcgtcgtg aatcactgaa cgatgttcca
    10501 tgcgaacaga tattcgatca tatcgaaata ttaaacatta cccctcccca cattgggcaa
    10561 aaaaaaacaa aaaacgatca attaacgatc aattagtatt gattcacata atctctcatc
    10621 attaatgaga acattgtcac atatttacaa tatagaactg ttacttttag caagctgtta
    10681 gtaaccaacc gttactaata attttaattc ttaatacttt gttcattcaa taaatgagtt
    10741 gagagaatat atgaataaaa tatactccct taaatatagt gctgccactg gcggactcat
    10801 tgctgtttct gaattagcga aaagagtttc tggtaaaaca aaccgaaaac ttgtagcaac
    10861 aatgttgtct ctggctgttg caggtacagt aaatgcagca aatattgata tatcaaatgt
    10921 atgggcgaga gactatcttg atcttgcaca aaataaaggt attttccagc ccggagcaac
    10981 agacgtaaca atcactttaa aaaacggaga taaattctct ttccataatc tctcaattcc
    11041 ggatttttct ggtgcagcag cgagtggcgc agctaccgca ataggaggtt cttatagtgt
    11101 tactgttgca cataacaaaa agaaccctca ggccgcagaa actcaggttt acgctcagtc
    11161 ttcttacaag gttgttgaca gaagaaattc caatgatttt gagattcaga ggttaaataa
    11221 atttgttgtg gaaacagtag gtgccacccc ggaagagacc aaccctacaa catattctga
    11281 tgcattagaa cgctacggta tagtcacttc tgacggttca aaaaaaatca taggttttcg
    11341 tgctggctct ggaggaacat catttattaa tggtgaatcc aaaatctcaa caaattcagc
    11401 atatagccat gacctgttaa gtgctagtct atttgaggtc acccaatggg actcatacgg
    11461 catgatgatt tataaaaatg ataaaacatt tcgtaatctt gaaatattcg gagacagcgg
    11521 ctctggagca tacttatatg ataacaaact agaaaaatgg gtattagtcg gaacaaccca
    11581 tggtattgcc agcgttaatg gtgaccaact gacatggata acaaaataca atgataaact
    11641 ggttagtgag ttaaaagata cctatagtca taaaataaat ctgaatggca ataatgtaac
    11701 cattaaaaac acagatataa cattacacca aaacaatgca gataccactg gtactcaaga
    11761 aaaaataact aaagacaaag atattgtgtt cacaaatggg ggaaatgttc tgtttaagga
    11821 taatttggat tttggtagcg gtggtattat ctttgacgaa ggccatgaat ataacataaa
    11881 cggtcaggga tttacattta aaggagcagg aattgatatc ggaaaagaaa gcattgtaaa
    11941 ctggaatgca ttgtattcca gtgatgatgt tttacacaaa ataggccccg gtactctgaa
    12001 tgttcaaaaa aaacaggggg caaatataaa gataggtgaa ggaaatgtta ttcttaatga
    12061 agaaggaaca tttaacaata tataccttgc aagcggaaat ggtaaggtaa tactaaataa
    12121 agataattcc cttggcaatg atcaatatgc ggggatattt tttactaaac gtggtggcac
    12181 gctagattta aatggacaca atcagacttt tactagaatt gccgccactg acgatggaac
    12241 aacaataact aactcagata caacgaaaga agccgttctg gcaatcaata acgaagactc
    12301 ctacatatat catgggaaca taaatggcaa tataaaacta acgcacaata ttaattctca
    12361 ggataagaaa actaatgcaa aattaattct ggatggtagt gtcaacacaa aaaatgatgt
    12421 tgaagtcagt aatgccagtc ttaccatgca aggccatgca acagagcatg caatattcag
    12481 aagcacaacg agtcattgct ccctggtatt tctttgtgga acggactggg tcaccgtttt
    12541 gaaagaaaca gagagttcat ataataaaaa gttcaattct gattacaaaa gtaataatca
    12601 gcagacctca tttgatcagc ctgactggaa aaccggggtg tttaaatttg atacattaca
    12661 cctgaacaat gctgactttt caatatcacg caatgccaat gttgaaggaa atatatcagc
    12721 aaataaatca gctatcacaa tcggcgataa aaatgcttac attgataatc ttgcagggaa
    12781 aaatattact aataatggtt ttgacttcaa acaaactatc agtactaatc tatccatagg
    12841 agaaactaaa tttacaggtg gcatcactgc acataacagc caaatagcca taggtgatca
    12901 agctgtagtt acacttaatg gtgcaacctt tctgaataat actcctataa gtatagataa
    12961 aggagcaaaa gttatagcac aaaattccat gttcacaaca aaaggtattg atatctccgg
    13021 tgaactgact atgatgggaa tccctgaaca gaatagtaaa actgtaacgc cgggtctcca
    13081 ctacgctgct gatggattca ggctgagtgg tggaaatgca aatttcattg ccagaaatat
    13141 ggcatctgtc accggaaata tttatgctga tgatgcagca accattactc tgggacagcc
    13201 tgaaactgaa acaccgacta tatcgtctgc ttatcaggca tgggcagaga ctcttttgta
    13261 tggctttgat accgcctatc gaggcgcaat aacagccccc aaagctacag ttagcatgaa
    13321 taatgcgatc tggcatctaa atagccagtc atcaattaat cgtctagaaa caaaagacag
    13381 tatggtgcgt tttactggtg acaatgggaa gtttacaacc cttacagtgg acaaccttac
    13441 tatagatgac agtgcatttg tgctgcgtgc aaatctggcc caagcagatc agcttgttgt
    13501 caataaatcg ttgtctggta aaaacaacct tctgttagtc gacttcattg agaaaaatgg
    13561 aaacagcaac ggactgaata tcgatctggt cagcgcacca aaaggaactg cagtagatgt
    13621 ctttaaagct acgactcgga gtattggctt cagtgatgta acaccggtta tcgagcaaaa
    13681 gaacgataca gacaaagcaa catggactct gatcggctat aaatctgtgg ccaacgccga
    13741 tgcggctaaa aaggcaacat tactgatgtc aggcggctat aaagccttcc ttgctgaggt
    13801 caacaacctt aacaaacgta tgggtgatct gcgtgacatt aacggtgagt ccggtgcatg
    13861 ggcccgaatc atgagcggaa ccgggtctgc cggcggtgga ttcagtgaca actacaccca
    13921 cgttcaggtc ggtgcggata acaaacatga actcgatggc cttgacctct tcaccggggt
    13981 gaccatgacc tataccgaca gccatgcagg cagtgatgcc ttcagtggtg aaacgaagtc
    14041 tgtgggtgcc ggtctctatg cctctgccat gtttgagtcc ggagcatata tcgacctcat
    14101 cggtaagtac gttcaccatg acaacgagta taccgcaact ttcgccggcc ttggcaccag
    14161 agactacagc tcccactcct ggtatgccgg tgcggaagtc ggttaccgtt accatgtaac
    14221 tgactctgca tggattgagc cgcaggcgga acttgtttac ggtgctgtat ccgggaaaca
    14281 gttctcctgg aaggaccagg gaatgaacct caccatgaag gataaggact ttaatccgct
    14341 gattgggcgt accggtgttg atgtgggtaa atccttctcc ggtaaggact ggaaagtcac
    14401 agcccgcgcc ggccttggct accagtttga cctgtttgcc aacggtgaaa ccgtactgcg
    14461 tgatgcgtcc ggtgagaaac gtatcaaagg tgaaaaagac ggtcgtatgc tcatgaatgt
    14521 tggtctcaat gccgaaattc gcgataatct tcgcttcggt cttgagtttg agaaatcggc
    14581 atttggtaaa tacaacgtgg ataacgcgat caacgccaac ttccgttact ctttctgata
    14641 acagcccggg ccgcgtttgc ggcccttctt ctaccggaga gaatatgtat taccctgtga
    14701 cagactatat cgctcttgct ctcattatta g
//
LOCUS       DAJTXY010000097        14690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTXY010000097 DAJTXY010000000
VERSION     DAJTXY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.097"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            85..366
                     /locus_tag="OO566_003018"
     CDS             85..366
                     /locus_tag="OO566_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606229.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVIAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHGKKPERLRR"
     gene            complement(1163..2623)
                     /gene="phoQ"
                     /locus_tag="OO566_003019"
     CDS             complement(1163..2623)
                     /gene="phoQ"
                     /locus_tag="OO566_003019"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6606230.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2623..3294)
                     /gene="phoP"
                     /locus_tag="OO566_003020"
     CDS             complement(2623..3294)
                     /gene="phoP"
                     /locus_tag="OO566_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6606231.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3463..4833)
                     /gene="purB"
                     /locus_tag="OO566_003021"
     CDS             complement(3463..4833)
                     /gene="purB"
                     /locus_tag="OO566_003021"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6606232.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4837..5478)
                     /gene="hflD"
                     /locus_tag="OO566_003022"
     CDS             complement(4837..5478)
                     /gene="hflD"
                     /locus_tag="OO566_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6606233.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5514..6620)
                     /gene="mnmA"
                     /locus_tag="OO566_003023"
     CDS             complement(5514..6620)
                     /gene="mnmA"
                     /locus_tag="OO566_003023"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6606234.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGHEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6674..7135)
                     /gene="nudJ"
                     /locus_tag="OO566_003024"
     CDS             complement(6674..7135)
                     /gene="nudJ"
                     /locus_tag="OO566_003024"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6606235.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7145..7798)
                     /gene="rluE"
                     /locus_tag="OO566_003025"
     CDS             complement(7145..7798)
                     /gene="rluE"
                     /locus_tag="OO566_003025"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6606236.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7970..9220
                     /gene="icd"
                     /locus_tag="OO566_003026"
     CDS             7970..9220
                     /gene="icd"
                     /locus_tag="OO566_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6606237.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9323..9646)
                     /gene="iraM"
                     /locus_tag="OO566_003027"
     CDS             complement(9323..9646)
                     /gene="iraM"
                     /locus_tag="OO566_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6606238.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(9899..9943)
                     /locus_tag="OO566_003028"
     CDS             complement(9899..9943)
                     /locus_tag="OO566_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606239.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            complement(10179..10289)
                     /locus_tag="OO566_003029"
     CDS             complement(10179..10289)
                     /locus_tag="OO566_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606240.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(10342..10746)
                     /locus_tag="OO566_003030"
     CDS             complement(10342..10746)
                     /locus_tag="OO566_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR6606241.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10967..11698)
                     /gene="bluR"
                     /locus_tag="OO566_003031"
     CDS             complement(10967..11698)
                     /gene="bluR"
                     /locus_tag="OO566_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCR6606242.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11903..13114)
                     /gene="bluF"
                     /locus_tag="OO566_003032"
     CDS             complement(11903..13114)
                     /gene="bluF"
                     /locus_tag="OO566_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCR6606243.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            13428..13664
                     /locus_tag="OO566_003033"
     CDS             13428..13664
                     /locus_tag="OO566_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCR6606244.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13707..13979
                     /locus_tag="OO566_003034"
     CDS             13707..13979
                     /locus_tag="OO566_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCR6606245.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            14008..14274
                     /gene="ariR"
                     /locus_tag="OO566_003035"
     CDS             14008..14274
                     /gene="ariR"
                     /locus_tag="OO566_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCR6606246.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            14387..14635
                     /locus_tag="OO566_003036"
     CDS             14387..14635
                     /locus_tag="OO566_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606247.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         3948 a         3350 c         3429 g         3963 t
ORIGIN      
        1 agcatgagga ttttgctttt gatataagcg acagccaccg ccagaaactg gaagaattta
       61 gcggggtgta atgaggtcag cagcatgaca gaagccgaaa taacgggatt aatccgccgc
      121 gttgccggaa tcagccagca ggctgacgaa caggccacgc agccggacag cgtgatagcc
      181 gaaaactatg cgcgtgttgt tgctgaggtg atgcgccgtg atggtataga acttaatggc
      241 gtggatatgc gcaacatacg aaccagagtt cttgagttgc tggcctaccg tcgccgcgtg
      301 gagatgtatc gggagaagga gaaaatcacg taccacggga agaagccgga gcggttacgg
      361 cggtaactca ctgagattaa agcaaagcgc aaaattgcgc tggctacatc cctcacatct
      421 gaggactgtt aaccagcagg ccggaatata ttcagaaact acgaaatttt cgcagtttaa
      481 ttactcgtca gcttaatcgc taacccgctc tgatattttc ggaaacctca atttgaggaa
      541 gtcggcgagg taactgtttg atattcccga tagcgcaaaa atgcgcggtc gggcagaatg
      601 accaccacct caatcgtgac ggagagtgac aaccagtgac acaccgtgac aaaaagacgg
      661 tttaaccgtt cattaaaaat cgacgtgatt ttgtgctttc cggatgacgt ggccaacgtc
      721 attttttagc gaaaaattct gattaaggtc actggctgaa cggtcataat gaccacgcag
      781 accatcgagg caaccatagc gatcatcgtt acggttggag cttttccgcc attggtggaa
      841 aaggtgcaca tctggatcat cattacggtg atcaagggta tgtaaaaccc accagcctga
      901 ttaaaggtta accggaaaaa aaatccggta gccccgttat ggggatcccc atatcgacat
      961 taacgcccct catgaattgg tgcgcttccc cctggaaaga ttacccgcct gtatatttct
     1021 tgtgtctatt tgttccacgt tgtttcatac agtgcaccga acggtgtagt taatggtgta
     1081 gtcaatttgc gatttttggc actttttcaa tgaaaattgt ttaatcgaat caatgacttg
     1141 atgtagtggt aaaaggacat atttattcat ctttcggcgc agaatgctgg cgaccaaaaa
     1201 tcacctccat ccgcgcaccg cccagcatgc tctctccggc gacgatttta ccctcatatt
     1261 gctcggtgat ttcgcgggct accgccagcc ctacaccttg cccagggcgt aaagtatcaa
     1321 cccgttgacc acggtcgaaa atgacctctc gcttgcttaa tggaataccg gggccatcat
     1381 cctcgaccac aatatagaga tgctcgtcgg tttgccttgc agaaatttcg acaaactcga
     1441 ggcaatattt acaggcatta tccagcacgt tgcccatcac ctcgacaaaa tcgttctgct
     1501 caccgacaaa gctgatctct ggcgaaatat cgagagagat attgacccct ttgcgttgat
     1561 acactttgtt cagcgctgag gtgagattgt ccagcagtgg ggcgaccgga tgcagctcgc
     1621 ggctgagcaa tgtcccgccg cgcatactgg cacgatgcag gtagtagcca atttgctgtg
     1681 aaatgcggct gatttgctcc agcattaccg gctcagcatc actgacgctc atcttttcac
     1741 tacgcagaga acgcagcgta ctttgcagca ccgccagtgg cgttttcaga ctatgggtca
     1801 ggtcggtgag cgtcgtacgg tatttgtcgt aacgttcgcg ttcacttttt aacaatcggt
     1861 tcaggtttcg taccagactg gtcagttctc gcgttgtggc tggattgagc aattcgcggt
     1921 tatgttcttc cagttcgcgg acttcttttg ccagggcttc gatggggcgt aaactccacc
     1981 aggcggcgac ccacagcagc gggatcacta acagcagatt ggctgagagt acatagataa
     2041 accagctcca gaccatatag gaacttttta gctccaccgg aatggtatcc accaccacaa
     2101 tggttaattt tggcatccgc gatgttgccg ggtagacgtt taccgccacc gagtgggtca
     2161 tctccgcgtc gtcatcatct tcccgcactt cctgcaactg ttgctgtatc gaatgatctc
     2221 cactcagcaa gaggctggta tcgttaacat ccgcttcaat ttcatgaaaa ccattcgatt
     2281 tcagccagtc aggctggatc attttcatca gccagggcac gtcacgttgc gcccataaaa
     2341 gctgcccgtt ctcatcataa attagcgtca tggtggggct ttgcttgtcg atattttcgg
     2401 gtaactcgac atgcaactta ttgttttccc actgcgcaag ggtatagaac agattgctct
     2461 cgccacgtaa cagccgaaac gtagttttat cgaaactgac gctataaccg atcagcgcga
     2521 ccattccgta ggcaagcgaa agcaccagta ctaccgctgc cgttgccaac agaaaacgta
     2581 cccgcagcga gagcgggaaa aaaagacgca gtaatttttt catcagcgca attcgaacag
     2641 gtagccctgg ccgcgaacgg tggtaatgac ttcttgcgga tattgtgcct gaattttttt
     2701 gcgcagacgt cccatcagta catcaatggt atggctttcc cgcagctccg catccggata
     2761 gagttgtagc attaacgaat ctttgctgac cactttgcca ttattgcgta tcaacgtttc
     2821 cataatggtg tattcgaacg cggtcagttt gatcacttcg tcattaatag ataattcacg
     2881 gcgagagaga tcaacctgaa acggggggag cgaaatgacc tgtgaagcca gaccgctatt
     2941 acgccgcatt aatgcctgca ttcgcgccat cacctcttca atatgaaacg gtttagtcac
     3001 gtaatcatca gcaccggcac ttaatacttc gactttgtcc tgccagcttt cacgggcggt
     3061 taataccaga atcggcagtg aaacatcgtt gctacgccag cggcgaatca gtgacagacc
     3121 gtcctcgtca ggcaatccga gatcgacaat cgcaatatcc ggtaaatgtt cattgagata
     3181 ataatcggct tctttggcat cttctgcgtc atcgacctga tgaccagcat cctgaatctg
     3241 aactttaagg tggtgacgta acaacgcatt gtcttcaaca accagtacgc gcatttttat
     3301 ttctccctgt cttaattatt aaaatagtgt aacgcgatta tgtgtttatg ggggtaaaca
     3361 ttaaataaac cagcggggag gggaggtaaa gtgaaaaaag aaaaagtgga taatcttaat
     3421 aagcaggccg gacagcatcg ccatccggca ctgatttgag gcttatttca gctcatcaac
     3481 catcgtgata gctcgaccaa tatagttagc cggcgtcatc gctttcaggc gggctttctc
     3541 ttcttctggc aacgccagac catcgataaa ctgcttcatg ccttcggcgt caacgcgctt
     3601 accgcgagta agctctttca gcttctcgta cggtttttcg atgccatagc gacgcataac
     3661 tgtctggatt ggttctgcca gcacttccca gttgtgatcc agctcatcca gcagatggtc
     3721 acggttcact tccagtttgc tcacgccttt cagggtggat tgatacgcaa tcagcgcata
     3781 accgataccc acgccgaggt tacgcagcac ggtggagtcg gtcaggtcac gctgccagcg
     3841 ggaaaccggc agtttgcttg ccagatgctg caataccgcg ttggaaaggc ccagattccc
     3901 ttcggagttt tcgaagtcga tcgggttaac tttatgcggc atggtggaag aaccaatctc
     3961 accagcaatg gttttctgtt tgaagtggtt aagggcgata taaccccaga cgtcacggtc
     4021 aaagtcgatc agaatggtgt tgaagcgcgc aacgcaatca aacagttcgg caatgtagtc
     4081 gtgcggttca atctgggtag tgtacgggtt ccattgaata cccagcgagg tgacgaactc
     4141 ttcgctgaac tgatgccagt caacttccgg gtaagcggcg atgtgggcgt tatagttacc
     4201 gaccgcgccg ttgattttgc cgaggatctc cacctggtta agctggcggt gctggcgctc
     4261 catacggtag gcgacgtttg ccatctcttt accgatggtt gacggcgtgg ctggctgacc
     4321 gtgggtacga gacagcagcg ggatatcgcg gtactgaacg gcgagatctt taatgccatc
     4381 aatcagttga cgccagtacg gcaggatcac ttcatcacgc gcggttttca gcattaatgc
     4441 gtgggagagg ttattgatat cttccgaagt acaggcaaag tggatgaatt cagaaaccgc
     4501 gtgcagttcc gggatctccg ccactttttc tttcaggaaa tactcaaccg ctttaacgtc
     4561 gtggttagtg gtacgctcga tggttttgat gcgtgcggca tcttcttcgc tgaaattggc
     4621 gacaattgca tcaaggtaac cgattgcgtc ggcagcaaaa gcaggaactt ccttgatcgc
     4681 tgcgtgcgcg gccagttttt gcagccaacg tacttcaact tgtacacgga atttcagcaa
     4741 accatattcg ctgaaaatcc cgcgcagcgc gctgagttta tcgccgtagc gtccatcgac
     4801 aggggaaacg gcggtcagtg aggataattc catagatcac aactccgggg ttaaatgagc
     4861 aagaatttgt tttgcctgag tggtcaggcg attacgagaa aacatcagtt gtagacgtcc
     4921 gccgccgacc tggtgccaga gcacggcggc gcgaatgcct gccagcaggg ttgcgcgaac
     4981 tttcgcctgc acttgtgggc tttgcagtac agcaggggaa ccggtgacct gaatgcgcgg
     5041 gccaagcggg ctgatcacat caacatagat agcagccatc gcgctcatca gcgtttcgga
     5101 ctgtaaatcg aagtgttcga gctggcgttg caggccgttg atccggttgc ccagagtgtc
     5161 gagcgcgcct ttcgctgagg agagtttgcg ctcaagcacc atcaagctga gtgtgtagcg
     5221 ggttaattcg gcgtttaagc cctggcaact gctggcattg agcacgccga gcagggtttc
     5281 cagcccgacg cgcaggttgg cttcgctacc gccaaaaacc gccagcgtcg agctggggtt
     5341 catatcaata atactgttga gtgagacgtg tagcgcatcg gcatcacaat gcccctggtg
     5401 agcgagttgt tgcaccaggc gtgccgactg acaaataccg gccagggcga gggtgatgtc
     5461 atagtaattc tttgccacgt tcactgcttc cttgttttaa gtaaagataa taatcagacc
     5521 ggcagcggca gacgctgctc aataataccg ccaccgaggc acacttcacc gttatagaag
     5581 acggcagact ggcccggcgt cacggcggca accggctcat cgaaaatcac tttaatgcga
     5641 tcgtcgtcca gcgccttgac ggtgcacgga atgtcggtct ggcgatagcg ggtttttacc
     5701 gtgcaacgca tagtgccggt gaatggttcg cgatcgaccc agtgcagctg ctgggcaatc
     5761 aacccgacag acatcagccg cgggtgttca tggccctgag cgacaaccag aatgttgttt
     5821 tcgacgtctt tgtccaccac ataccacggt tcttcggtgc cttctttggt gccaccgata
     5881 cccagacctt tacgctgacc gagagtgtga tacatcagcc cctggtgctc accaatttca
     5941 tggccatcga cggtaatgat tttgcccggt tgcgccggga gataacggcc caggaactcg
     6001 cggaatttac gttcgccaat gaagcaaatg ccggtagagt cttttttctt cgcggtgacc
     6061 agaccaagat cttcagcaat cttacgcacc tgcggttttt ccagttcgcc gaccgggaac
     6121 aggctttgcg caatctgctc atggctgagc gtataaagga agtagctctg gtctttattg
     6181 ctgtccagac cacgcagcag gcggctcttg ccatcgacat cggcccgacg tacgtaatga
     6241 ccggtagcga tataatcggc acctaaatct tcggcggcaa attcgaggaa ggctttaaat
     6301 ttgatctcct tgttgcacag aatatccgga ttcggcgtgc gaccggcttt atattcggca
     6361 aggaacagtt cgaagacgtt gtcccagtac tcggcagcaa agttaacggt gtgcagttca
     6421 atgccgagct tgtcgcagac cgcctgggca tcagccagat ccgctgccgc tgtgcaatat
     6481 tcctcaccgt cgtcttcttc ccagttcttc ataaacaggc cttcgacctg atatccctgt
     6541 tgttgcaaca gccaagcaga aacggaggaa tcgacaccgc cggacatgcc gacgattact
     6601 ttttttgcgg tttcagacat tggatcactc acgacattga acttcaaggc ggcgtattct
     6661 atcacgcagc cttttagatg acaccctttg taaaaggcca gttaaaatcg ccaatcatct
     6721 ccagcggata acgttgcccg ctttgataac aacgaatact ttccgccacc agcggcgaac
     6781 gaagatttga cgcctgtaaa atttcttcgg cgctgaccca acggcagcag tcgatatcgc
     6841 tgtcatgagg ctgggtcggg catatttgct caagctcaat ggcaaagagg aaacgcaaaa
     6901 acggcgtttt atctggcgca atccactgat gcatacgaat aaagtgttgc ggctgcgcgc
     6961 tgatgccggt ttcttcccac agttcacggg cggcggcttc cactaaggtt tcatcggctt
     7021 ccagatgccc ggcaggttgg ttccataacg ctttaccatt aatcgtctct tcaacgacta
     7081 aaaatttgcc ttctgcgtgc accacgcaag caacggtaac gtgcggttta aacattttgc
     7141 atccttaatc tgtcacttct cgccattcgc cattggcaag attatccaaa gagtaatctc
     7201 ccatcgcata gcgaatcagt cgcagcgtgg ggaaaccaac atgggcggtc atgcggcgca
     7261 cctggcgatt acgtccttca tataaggtga tcttcagcca gctggtggga atacttttgc
     7321 gttcacgaat tggtggattc cgcggccata accacgcggg ttcgtcaacc agctccgcgc
     7381 ctgcgggcag ggtagggcca tcatttaagg ttacgccatt gcgcaaggct tcaagtgcgt
     7441 cttgtgtggg aataccttcc acctgcacat aatagatttt tccggtgcgt ttacccggct
     7501 gggttaaacg cgcctgcaac gcgccgttat tggtcagcac cagcaaccct tcgctatcgc
     7561 ggtcaaggcg acctgctgca taaacgccct gaaccgggat gaattctttt aatgttttgc
     7621 gtccggcttc atcggtgaac tgcggaagaa catcgtaggg tttattgaag aggatcacac
     7681 gcgtgggctg gttttcatgt ttacgcctgg tagaacgttg cgagctgaat cgcttaacct
     7741 ggtgatttct aaaagaagtt ttttgcatgg tattttcaga gattatgaat tgccgcatta
     7801 tagcctaata acgcgcatct ttcatgacgg caaacaatag ggtagtattg acaagtcaat
     7861 tacaaatcat taacaaaaaa ttgctctaaa gcatccgtat cgcaggacgc aaacgcatat
     7921 gcaacgtggt ggcagacgag caaaccagta gcgctcgaag gagaggtgaa tggaaagtaa
     7981 agtagttgtt ccggcacaag gcaagaagat caccctgcaa aacggcaaac tcaacgttcc
     8041 tgaaaatccg attatccctt acattgaagg tgatggaatc ggtgtagatg taaccccagc
     8101 catgctgaaa gtggtcgacg ctgcagtcga gaaagcctat aaaggcgagc gtaaaatctc
     8161 ctggatggaa atttacaccg gtgaaaaatc cacacaggtt tatggtcagg atgtctggct
     8221 gcctgctgaa acccttgatc tgattcgtga atatcgagtt gccattaaag gcccgctgac
     8281 cactccggtt ggtggcggta ttcgttctct caacgttgcc ctgcgtcagg aactggatct
     8341 ctacatctgc ctgcgtccgg tacgttacta tcagggcact ccaagcccgg ttaaacaccc
     8401 tgaactgacc gatatggtta tcttccgtga aaactcggaa gacatttatg cgggtatcga
     8461 atggaaagct gactctgccg acgccgagaa agtgattaaa ttcctgcgtg aagagatggg
     8521 cgtgaagaaa attcgcttcc cggaacattg cggtatcggt attaagccgt gttctgaaga
     8581 aggcaccaaa cgtctggttc gtgcagcgat cgaatacgca attgctaacg atcgtgactc
     8641 tgtgaccctg gtgcacaaag gcaacatcat gaagttcacc gaaggcgcgt ttaaagactg
     8701 gggctaccag ctggcgcgtg aagaatttgg cggtgaactg atcgacggcg gcccgtggct
     8761 gaaagttaaa aacccgaata ccggcaaaga gatcgtcata aaagacgtga ttgctgatgc
     8821 attcctgcaa cagatcctgc tgcgtccggc tgaatatgat gttatcgcct gtatgaacct
     8881 gaacggtgac tacatttctg acgctctggc agcgcaggtt ggcggtatcg gtatcgcccc
     8941 tggagcaaac atcggtgacg aatgcgccct gtttgaagcc acccacggta ctgcgccgaa
     9001 atacgccggt caggacaaag taaaccctgg ctctattatt ctctccgctg agatgatgtt
     9061 acgccatatg ggctggactg aagccgcaga cctgattgtt aaaggtatgg aaggcgcgat
     9121 caacgccaag accgtaacct atgacttcga gcgtctgatg gaaggcgcta aactgctgaa
     9181 atgttcagag tttggtgatg cgatcatcaa gaatatgtaa tcactacatg tgttaaatat
     9241 ttaaacgggc gtataacacg cccgttgttt tatttatgtg gatattatta atagcatatc
     9301 gagcatattt atataaaacc tattacttga gcccatatgg gcatattttt ataatacaac
     9361 tcttatgcaa acatttattt gttatttcgc tttctcctgg aggacactct tgactgcttt
     9421 tgagtaaact ccataaattc ttgttgaatg gtgcaatgtt ataaatagta ataggatatt
     9481 ctttttcctt aaggataata ccaagcttaa ccggtgtaaa tatccttcca ggaggaagaa
     9541 atatagtaga ttgataccag atgatcattt tcatatcacc ccatatggct gaaaaagata
     9601 tgccgcatgt aggttgaatt accgtgtcaa ttactaccca cttcatttgt tatgtcttat
     9661 cccacggtat ttaatatagt tcatttggat gttcatttct ttattttgca tatgagtata
     9721 ttaccccttc aaaaaataaa ttaattaaaa cgattgctta tataaaacaa aatttaaatc
     9781 aaggaatctc aatggatgtt aaacaaaatg agatttagtg aaaacaataa attattcact
     9841 tcgttttaga tttgtttagc tataatgtta tacattcaaa tgactgaaca tcctgtaatt
     9901 aaaacatagc ctttatgcta cttggtgcca atttgctaaa cattatggtt gcctttttat
     9961 ataacgataa taatgaatat aaacatgaca tgagaataag gtttcaattt ttgagttata
    10021 taggaaaggt ttaacctgtt cctggctaaa atacatataa ctggatgatg actaaaccaa
    10081 aacacatatg cgttaagtat tgaaacgggc gtatggcaca cccgttgttt ttataaatat
    10141 attaaccgtt ataaaataac gtataaaaag tcaagtgatc acatttcaaa tatcaatttg
    10201 atagtatggg cttggtgatt atttatgggt agcaataaaa ggacagtatt tatcatccat
    10261 agggatagtc tctgtacttt tattcccatt atgctaatgc cttactgaat tatgaagcat
    10321 ttcttaagca tccaacttta gctagattaa tggtttatta ttttctacat cttcaatata
    10381 taaaagcgta ttatcaatgg cgtagtaact gcgtttgtta tgattaacat cagtaaccca
    10441 acggaaaacg cccgcgcctg ctagtgttga acagtattcc cgaaatgtag attttccgca
    10501 aatatgaagc aatgcggcct cttttatttt agcaggattc ttggtcgtac taacttttaa
    10561 ctggttcctg gttcctctta ataacaaaac cgtgtcatcg tgagtaataa ttctgatgtt
    10621 atccgtagcc agataataaa tgtaatgtgc aatacggtga tgttttaatt ctgaataaaa
    10681 ccaggagaag ttttgctctt ttctcacttg ctcaaacatc ttttgaaaaa caacgacctg
    10741 atccatcagg ataataacct cttgttagtt gtgagactgc gtagtgtgca cgatcggttt
    10801 taccacttca atctggtctg tcctttggct gtgatatgta cagagtgtga tagagggatt
    10861 acctgaattc tcccggtgag cattttgcaa cggaccagct ccggtacaaa tgctgttgtg
    10921 ggttcagatt ataacattct gtctaagggg cgggataaag gtgaaattag ggggcatgaa
    10981 agatgacttt ataaccttgc tcaccccaat gttgtaaaag ttcgttttgc cttctcgttg
    11041 gtgccatgcc tgtccagaca atcaatgttt gcgtcgggaa cagttccggg cgcggcgatt
    11101 caatgggatc agcaagaaca gaaatgtgcc atcctgacaa tgataaacgc catgcttcca
    11161 gccacaatcg tgctctctct tcaacattcc acgccatcag aatggcttct ttaccgggct
    11221 tacggcgcgt ttcgaaaagc gaggttgctg cgtattcaat taatgcgccg tcaaacatac
    11281 tgctcataat gcgggaggtg ttgtgatcaa gcacgagacg ctggcgaaca ggaaggtaaa
    11341 catgattaat caattgatct actgggtact ctcgacccag tgaaataatt ctcgcgcgta
    11401 gtttggcagg attagccata cgaagaattg acatcatctc ttcttgcagg cggctccagt
    11461 catcttccgt atcctggctg gtggtttcca gtaatgcttt aactttgcct acagggacgc
    11521 cattactgat ccaacgcttg atctcttcga tgcgttgtat gtcttcttca tcaaagagtc
    11581 ggtgtccgcc ttcactgcgc tgtggtttta acaaaccgta gtggcgttgc caggcccgga
    11641 gagtgacagg attaatcccg caacgttcag caacatcacc aatgctgtaa taagccacaa
    11701 ttcctccttg cggtcacaaa tctccgtcgc cggtacacga cccaataata ctttgtacaa
    11761 tatacgctaa aattgtacaa agtataaata agatttcagc taaattggat gaaacattat
    11821 ttttaatgta gattaaattt aaacataacg tattcatttt cacgatgatt tactgaaatc
    11881 atgtgaaaga atgtgctgaa aattattttt tctctggcca cgctatggaa gggataccat
    11941 tcaatttagc tttagcaaac agatctccct gaaacatctc aattcctgcg gattcaagcc
    12001 acatccactc ttctggtgtt gccacgccca tagcactgac ttgaatttca agtgatgtac
    12061 agcattttat gatcgcttga ataattgcct gccgtggccc gcttttatga acattggtaa
    12121 tcaattcctg actgatttta attctgtcag gctggaagcg tgacaggagt aacaaaccgg
    12181 caaaacctgc gccaaaatga tcaattgcta cactgatacc ggcagcctta agcgatttaa
    12241 tcgcttctgc aaactcatca aaccgagata taacttcact ttcagtaaat tcaacgatga
    12301 tttgttcagg caccagagca ttggccttta tttcatcaag taaaaaagag actgcgtcag
    12361 gttcgttaac cagggtcata ggtaatagat tgattgaaat cattttatca ccgagctcaa
    12421 gtgcgtgtgt catcgcgaat gcaagcgcct tacttttgag atccgctgtg tagatttccc
    12481 cgtctttacg ctgcccaacc gctatggctg atgggctatc ttcatttttt tgcacaatgg
    12541 cttcaaaagc gattatccgc cgcgataagg gatcgacaat aggatgaaag gcaaagtggt
    12601 cgtttatagt tggtgaaagg gtacaggaat caagttcttt atcagatccg tcagcgataa
    12661 aaagccagga gtcttcggca gggatctcga aataggttga ttgttcggtt gcaaggacaa
    12721 aagtacgaaa aaattgtagc gctctgtcat cataagttag ctgaaatttt gatgtgcctt
    12781 tgtcgaatac ggcctgtaaa acgtcatctc gctcgtgcag gcgcaaatca aataattcca
    12841 ttcccgcttt gccaaaacgg cgagcaggcg cgtaatcgca cagcagctca acaatattat
    12901 agtgccgtgg atcttggcat atagcccgat atatcatttt aacctgttct tccggacctt
    12961 ccagaagctg gaaaaaatga gaaccattaa acagtaagat ccctgttacg tcagactgca
    13021 tgttcctgcg atttgctatc gaaaccattt cttcgatttt tttgacaggt tcgtcgtcac
    13081 gtatatggct acgataaata agggtggtaa gcattaacaa tccagggtaa tgggtgaggc
    13141 gagagtaaga cggtaacaga catatcttct tgtgtctttc ttttaatacc aaaacataac
    13201 cgtttcttta cattgataaa aaatggaaaa agttgaacac gagttggcga aaaatcttgt
    13261 atagattgtc agttaaatga tgcaatatgt tttatcataa cacattgttt tatatacatt
    13321 agtactaatt gcaaaaaatt aatttatcat tctgtacaca tatttcgtac aagtttgcta
    13381 ttgttacctc acttcacatt gattaacatt tttaacagag gcgtagcatg catcaaaatt
    13441 cagtgacttc agattctgcg ggagcaatca ctcgttactt tgcaaaagct aacttgccta
    13501 ctcagcagga aactctcggg gaaattgtga ctgagatttt gaaagatgga cgtaatctga
    13561 gtcgaaagtc gcattgtgcc aaacttcttt gccgactgga acaagcgaca ggggaagagg
    13621 aacagaaaca ttataatgca ctaattgggt tgctttttga ataatgcaac agcctgatta
    13681 tttaacaggt tagctatgaa gtcgttatga agacatctga taatgaacgt ataaaatatg
    13741 aaattactgg ccaggcggtg ctccagatac tgcgcatgaa gataaatttt tcattgcaga
    13801 ctcttattaa gcaattactc gtaatgaaat cggctgagga agatgctttc cgacgcgatt
    13861 taattgacag cataattcgt gattttagca atagtgattc aggagggcca aaccgaagaa
    13921 cagcgacagc tgacaataaa agtatgttca atggtaagaa aataaacaga atacattaaa
    13981 atttcataag taagatgaga ggttaccatg cttgaagata ctacaattca taatgcaata
    14041 actgataaag cgttagcaag ttactttcgc agttcgggta atttgttaga agaagagtca
    14101 gcagtgttag ggcaggctgt caccaattta atgctttcag gcgataatgt taataataaa
    14161 aatattatct taagtctgat acactccctg gaaacaacaa gtgatattct caaagctgat
    14221 gtgattagaa aaacactgga aatcgtgttg cgatacacag ctgatgatat gtaacttcta
    14281 attattaagt ataagtttat agaaaactca ttcatcttat ttttgtctgt cgctttagac
    14341 tatacacagc ataattttat tgggttaata tttctacgag gctgacatga ataattcaat
    14401 cccagagaga tttatttttc aatgtgcttt gtttaaaaat ctcgagaggg aggtgttcat
    14461 gacgcatggg tatgttgata gtcatattat tgatcaggct ttacgcttgc gtttaaaaga
    14521 tgaaaccagt gtgattcttt ctgatctcta tctgcaaata ttgcagtaca ttgaaatgca
    14581 taagactaca ctaacggata tcattattaa tgacagggaa tccgtgctct cttagaactt
    14641 gagtaaggtt ttagaataaa catcagctgt atcaccatgc tgatgcaaag
//
LOCUS       DAJTXY010000098        14686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTXY010000098 DAJTXY010000000
VERSION     DAJTXY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.098"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            117..1418
                     /gene="rstB"
                     /locus_tag="OO566_003037"
     CDS             117..1418
                     /gene="rstB"
                     /locus_tag="OO566_003037"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6606248.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1494..2423
                     /gene="tus"
                     /locus_tag="OO566_003038"
     CDS             1494..2423
                     /gene="tus"
                     /locus_tag="OO566_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6606249.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2420..3823)
                     /gene="fumC"
                     /locus_tag="OO566_003039"
     CDS             complement(2420..3823)
                     /gene="fumC"
                     /locus_tag="OO566_003039"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6606250.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3966..5612)
                     /gene="fumB"
                     /locus_tag="OO566_003040"
     CDS             complement(3966..5612)
                     /gene="fumB"
                     /locus_tag="OO566_003040"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6606251.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5811..6986
                     /gene="manA"
                     /locus_tag="OO566_003041"
     CDS             5811..6986
                     /gene="manA"
                     /locus_tag="OO566_003041"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6606252.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLRDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANKSPVTVKGHGRLARVYNKL"
     gene            7087..8595
                     /locus_tag="OO566_003042"
     CDS             7087..8595
                     /locus_tag="OO566_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6606253.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8821..10086)
                     /gene="uidC"
                     /locus_tag="OO566_003043"
     CDS             complement(8821..10086)
                     /gene="uidC"
                     /locus_tag="OO566_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6606254.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSIRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10125..10292)
                     /locus_tag="OO566_003044"
     CDS             complement(10125..10292)
                     /locus_tag="OO566_003044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6606255.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(10308..11498)
                     /gene="uidB"
                     /locus_tag="OO566_003045"
     CDS             complement(10308..11498)
                     /gene="uidB"
                     /locus_tag="OO566_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6606256.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQV"
     gene            complement(11495..13306)
                     /gene="uidA"
                     /locus_tag="OO566_003046"
     CDS             complement(11495..13306)
                     /gene="uidA"
                     /locus_tag="OO566_003046"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6606257.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13694..14284)
                     /gene="uidR"
                     /locus_tag="OO566_003047"
     CDS             complement(13694..14284)
                     /gene="uidR"
                     /locus_tag="OO566_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6606258.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14513..>14686)
                     /locus_tag="OO566_003048"
     CDS             complement(14513..>14686)
                     /locus_tag="OO566_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6606259.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3686 a         3820 c         3495 g         3685 t
ORIGIN      
        1 gatacatccg tctgttccgg acattatctt ccaaacaagg tcttatccag ttggcggttt
       61 acattgctcc acagtcgtga attaatacga atagtattct gttgagggcg atgccaatga
      121 aaaaattgtt tatccagttt tacctgttat tgtttgtctg cttccttgtg atgtctctgc
      181 tggttgggct ggtgtacaaa tttaccgccg aacgcgcggg caaacagtcg ctggatgatt
      241 tgatgaacag ttcgctgtat ctgatgcgca gcgaattgcg agagatcccc ccacacgact
      301 ggggtaagac gctgaaagag atggatttaa atctctcttt cgatctgcgt gtcgagccac
      361 tgagtaaata ccatcttgat gatatttcca tgcaccgact gcgtggcggc gaaattgtcg
      421 ccctggacga tcagtacacg tttttgcagc gtatcccgcg cagccactac gtgctggcag
      481 ttggtcctgt tccttatctt tattacctcc atcaaatgcg attgctggat atcgccctga
      541 tcgcttttat tgctatttcc ctcgcctttc cggtgtttat ctggatgcgt ccgcactggc
      601 aggatatgtt aaaactggaa gcagcggcgc aacgatttgg cgatgggcat ctcaatgaac
      661 gtatccactt tgatgagggt tcgagctttg aacgacttgg tatcgcattt aaccagatgg
      721 cggacaatat caacgcctta attgccagca aaaaacagct tattgacggt atcgctcacg
      781 aactgcgaac accgttagtg cgcctgcgtt atcgactgga gatgagcgat aacctgagcg
      841 ccgccgaatc ccaggcgttg aatcgtgata tcagtcaact tgaagcttta attgaagagc
      901 tgctgactta tgcccgactc gatcgcccac aaaacgagct tcatcttagc gaaccagacc
      961 tgccgttgtg gctgtcaacg catctggcag atattcaggc agtaacgccc gataaaacgg
     1021 tacggataaa aacgctcatg caaggccatt atgcggcgtt ggatatgcgc ttaatggagc
     1081 gcgtgctgga taatttgctc aataacgccc tgcgctactg ccattcaacg gttgaaacca
     1141 gcctgctact gtcggggaat agagcgacat taattgttga ggatgatggc ccagggattg
     1201 ccccagaaaa ccgcgaacat atctttgaac cttttgttcg cctcgatccc agccgggatc
     1261 gctcaaccgg cggctgcggg ctggggctgg caattgtcca ctctatagca ctggcaatgg
     1321 gcggtacggt taattgtgac atcagcgaac tgggtggtgc tcgcttctcg tttagctggc
     1381 cgttatggca taacatcccg caatttacct ctgcctgaca ctacgcgcac gatggtcaag
     1441 tcacaacgac tgtgctataa aataagtatg ttgtaactaa agtggttaat attatggcgc
     1501 gttacgatct cgtagaccga ctcaacacta cctttcgcca gatggaacaa gagctggcta
     1561 catttgccgc tcatcttgag caacacaagc tattggttgc ccgcgtgttc tctttgccgg
     1621 aggtaaaaaa agaggatgag cataatccgc ttaatcgtat tgaggtaaaa caacatctcg
     1681 gcaacgacgc gcagtcgctg gcgttgcgtc atttccgcca tttatttatt caacaacagt
     1741 ccgaaaatcg cagcagcaag gccgctgtcc gtctgcctgg cgtgttgtgt taccaggtcg
     1801 ataacctttc gcaagcagcg ttggtcagtc atattcagca catcaataaa ctcaagacca
     1861 cgttcgagca tatcgtcacg gttgaatcag aactccccac cgcggcacgt tttgaatggg
     1921 tgcatcgtca tttgccgggg ctgatcaccc ttaatgctta ccgcacgctc accgttctgc
     1981 acgaccccgc cactttacgc tttggttggg ctaataaaca tatcattaag aatttgcatc
     2041 gtgatgaagt cctggcacag ctggaaaaaa gcctgaaatc accacgtagt gtcgcaccgt
     2101 ggacgcgcga ggagtggcaa agaaaactgg agcgagagta tcaggatatc gctgccctgc
     2161 cacagaacgc gaagttaaaa atcaaacgtc cagtgaaggt gcagccgatt gcccgcgtct
     2221 ggtacaaagg agatcaaaaa caagtccaac acgcctgccc tacaccactg attgcactga
     2281 ttaatcggga taatggcgcg ggcgtgccgg acgttggtga gttgttaaat tacgatgccg
     2341 acaatgtgca gcaccgttat aaacctcaag cgcagccgct tcgtttgatc attccacggc
     2401 tgcacctgta tgttgcagat taacgcccgg cttttatact gccgaccatc tgttctggcc
     2461 gtacccagct gtcaaactcg gcttcgctaa gataccccag cgcaagggcc gcagctttta
     2521 aggtcagccc ttctttatgc gcttttttgg cgatctccgc ggctttgtca taaccaatgt
     2581 gggtgttaag cgcagtcacc agcatcagcg attcattgag taattgattg attcgctcac
     2641 gattcggttc aatacccact gcgcagtgtt tgttaaaact ttccatgcca tctgccagca
     2701 agcgcaccga ttgcaggaaa ttatggatca ccatcggacg gaagacgttc agttcaaagt
     2761 taccggaagc gccacccatg ttgatcgcca cgtcgttccc cattacctga cagcagagca
     2821 tggttaatgc ttcacactgg gttgggttca ctttccccgg catgattgag ctgcccggct
     2881 cattttccgg gattgagatt tcaccaattc cgcagcgcgg gccagaggcc agccagcgga
     2941 catcattggc gattttcatc agtgacgcag ccaacccttt caacgcgccg tgcgcctgaa
     3001 ccagggcatc acaggtcgca agcgcttcaa atttgttcgg cgcggtaaca aacggtgcac
     3061 aggtaatgac tgccagttca tctgctacgc gccgcgcata ctccggatgg gtatttagtc
     3121 cagtacccac cgctgtaccg ccaagagcca gttccgctac gtgaggcagg ctgtattcga
     3181 tatgtttgag attatgctcg agcatcgcta cccagccgga aatctcctgc cctagtgtta
     3241 gcggcgtggc gtcctgcaag tgggttcgac cgattttgac gatatcggca aatgcacgcg
     3301 atttttcact cagcgtctgt gtcagggttt taagctgcgg aatgagttgc ttgcgcagtg
     3361 ccagcagcgc cgcaacgtgc atcgccgtcg gaaagacatc gttggaactt tggcttttgt
     3421 tcacgtcgtc gttagggtga actttacgtt ccatcccgcg cacaccgccg agtaattcac
     3481 tggcccggtt agccagcact tcgttcatgt tcatgttact ttgcgtgccg gagccggtct
     3541 gccagatagc cagcgggaat tcgtcgtcat gctgtcctgc cagtacttca tccgccgcct
     3601 gccgaatggc gctcgctttc tcttcagaca acaagtctaa atcttcatta acttttgccg
     3661 ctgcgcgctt ggttagcgcc agcgcatgaa tcagtgaggt gggcattttc tccgtcgaaa
     3721 tgcggaaatg ctccagcgag cgttgagttt gtgcgcccca cagcttatct gccgggacat
     3781 caatcgcccc catcgaatct ttttcgctgc gtactgtatt catggcctgc tcctcacctg
     3841 attaattttt tctttctgtt ttgctttcgt taagcaactt ttagctcact cattatttac
     3901 tatttgataa caaatgtttg gtctttcgtg ccatgtaaaa aaaccgcccc gaagggcggc
     3961 tctgtttatt tcacacagcg agtgcactgt gtgagttgta tctgctggaa gaagtcattt
     4021 cctttatcat ccacaaggat aaacgccggg aaatcttcca cttcaatttt ccagatggct
     4081 tccattccca gttccggata ttcaacacat tccaggctct taatacttcc ctgcgccaat
     4141 acagcggccg gaccaccgat actgccaagg tagaagccgc cgtgtttttt acaggcatcc
     4201 gtcacctgct ggctgcggtt gcctttcgcc agcatgatca tacttccgcc ctgcgcttgc
     4261 agttgatcga cataagaatc catccgtccg gcggtcgttg ggccaagaga accggaggca
     4321 taaccttccg gcgttttggc cggacccgcg tagtaaatcg gatgatcttt aatgtattgc
     4381 ggcagccctt caccgttatc catccgctct ttcagtttgg cgtgagcaat atcacgacca
     4441 acgataatcg tgccgttaag cgataagcgt gtagaaacgg gatactgcga caactgtgcg
     4501 aggatctctt tcatcggacg gttaaggtca acgcgcaccg cttcgccttc tcccgcttta
     4561 cgcagctctt cagggatata tttacctgga ttatgttcca gtttttcgat ccagatcccc
     4621 tgacggttga tcttcgcttt gatattacgg tcagcagagc aggaaacacc cataccaacc
     4681 ggacaggatg cgccgtgacg tggcaggcga atcacgcgga tgtcgtgagc gaagtattta
     4741 ccaccaaact gcgcacccag accgagattt tgcgcttcga tcagtaattc tttttccagt
     4801 tccacatcgc ggaacgcctg accgtgctca ttcccttccg ttggcagttc atcatagtat
     4861 ttcgcggaag ccagtttcac cgttttaagg ttcgtttctg cagaagttcc accaataacg
     4921 aacgcaatat gatacggagg acaggccgcg gtacccagcg tgcgcatctt ctcaaccagg
     4981 taatttttca gtttccccgg cgtcagtaac gctttggttt cctgatagag atacgtcttg
     5041 tttgccgaac caccaccttt tgcgatacag aggaatttgt attcgtcgcc atcaacggca
     5101 taaagatcga tctgcgctgg cagattggtg ccggtattca cttctttata catatccagc
     5161 ggcgcgtttt gcgagtagcg cagattatct tcgatataag tgttatagac accgcgcgcc
     5221 agcgccgctt catcaccacc accggtccat acacgctgcc cttttttacc aacaataatc
     5281 gcggtgccgg tatcctgaca ggttggcaga acgcctttcg ccgcgatgtc ggagttacgc
     5341 aggaattgca gcgccacata tttatcattt tcgctggcct ccgggtcacg cagaatgtcg
     5401 gccacctgtt gttggtgagc cggacgcagc ataaacgacg catcatgaaa cgcctgacgc
     5461 gccaacagag ttaacgcttc gggtgcgact ttcaaaatct cctgcccttc aaattcagat
     5521 acgctaacgt gttcactggt tagcaggtaa tactcagtat catctttttt gagtggaaaa
     5581 ggagactgat aatgaaaggg tttgtttgac attgttctct cacttactgc ctggtttggt
     5641 tatgctctgg gcgggtgttc cgttgccctg ttaaaagcga gtaacaatat cctacacagt
     5701 tttttaacaa aaactgagac tagtacgact ttttgcggct ccaggttact tcccgtagga
     5761 ttcttgcttt aatagcggga ttaatttcca cattaaaaca gggattgatc atgcaaaaac
     5821 tcattaactc agtgcaaaac tatgcctggg gtagcaaaac ggcgttgact gaactttatg
     5881 gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca catccgaaaa
     5941 gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat gtgattgaga
     6001 gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa ctgcctttcc
     6061 tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca aacaaacaca
     6121 attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat gccgccgagc
     6181 gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg cctttccttg
     6241 cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct actccagccg gtcgccggtg
     6301 cacatccggc gattgctcac tttttacaac agccacatgc cgaacgttta agcgaactgt
     6361 tcgccaacct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg attttaaaat
     6421 cggcgctcga cagtcagcag ggtgaaccgt ggcaaacgat tcgtttaatt tctgaatttt
     6481 acccggaaga cagcggcctg ttctccccgc tgttactgaa tgtggtgaaa ttgaatcctg
     6541 gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg
     6601 aagtgatggc gaactccgat aacgtgctgc gtgcaggtct gacgcctaaa tacattgata
     6661 ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag ttgttgaccc
     6721 agccggtgaa acaaggtgca gaactgggct tcccgattcc tgtggatgat tttgccttct
     6781 cgctgcatga ccttagggat aaagaaacca ccatcagcca gcagagtgcc gccattttgt
     6841 tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag cttaaaccgg
     6901 gtgaatcagc gtttattgcc gccaacaaat caccggtgac tgtcaaaggc cacggccgtt
     6961 tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa aattaacatc tcttgctaag
     7021 cttattaaag gcttataaca ccttcaggcg gccagtccgc ctgatttcat tttatggata
     7081 atcattatga ataaatcgct ggtagcggta ggcgtcattg ttgcgctagg cgtagtctgg
     7141 acaggcggcg catggtatac aggcaagaag attgaaaccc atctcgaaga catggtcgcg
     7201 caggcgaacg cgcaactcaa actgacagct cctgaatcca acctggaagt gagttatcaa
     7261 aactatcatc gcggcgtatt cagcagccag ttgcaactgt tggtgaaacc cattgctggg
     7321 aaagaaaatc cgtggattaa aagcggtcag agcgtcatct tcaacgaatc ggttgatcat
     7381 ggtcccttcc cgcttgccca gcttaaaaaa ctgaacctga tcccgtcgat ggcatcaatt
     7441 caaaccacgc tggttaataa cgaagtaagc aaaccactgt ttgatatggc aaaaggtgaa
     7501 acgccttttg agattaactc gcgcattggt tacagcggtg attccagttc cgatatttcg
     7561 ctcaatccac tgaattacga gcaaaaggat gaaaaagtcg cctttagcgg cggcgagttc
     7621 cagttaaatg ctgacagaga cggcaaagcc atctcccttt ccggggaggc gcaaagtggt
     7681 cggatagacg cagttaacga atacaaccag aaagtgcagt tgacctttaa taatctgaaa
     7741 accgacggtt ccagcacgct gggaagtttt ggtgagcgcg taggaaacca aaaactgtca
     7801 ctggaaaaaa tgaccatttc agtggaaggc aaagaactgg cactgctgga aggcatggag
     7861 atcagcggta aatcggatct ggtcaatgac ggtaaaacga tcaatagcca actggattac
     7921 tcgctaaaca gcctgaaggt acagaatcag gatctgggca gcggcaagct gactttaaaa
     7981 gtcggccaga ttgatggtga agcctggcat cagtttagcc agcaatataa cgcgcaaact
     8041 caggcgctgc tggcacagcc agaaattgcc aacaatcccg aactttatca ggagaaagtg
     8101 acggaagcct tctttagcgc cctgccgctg atgttgaaag gcgatccggt gattactatc
     8161 gcgccgctaa gctggaaaaa cagtcagggt gaaagtgcgc tgaatctgtc gctgttcctg
     8221 aaagatccgg caacgactaa agaagcgccg caaacgctgg cgcaggaagt agatcgttcg
     8281 gttaaatctc tggatgcgaa actgaccatt ccggtggata tggcaactga gttgatgact
     8341 caggtagcga agctggaagg ttatcaggaa gatcaagcga aaaaactggc gaaacagcaa
     8401 gttgaaggtg catcagcaat ggggcagatg ttccgtctga ccaccttgca ggacaatacc
     8461 atcaccacca gcctgcaata tgctaacggt cagataacgt taaatgggca gaaaatgtcg
     8521 ttagaagatt ttgttggcat gtttgcgatg ccagctctta acgttccggc tgtaccggca
     8581 attccgcagc agtaattcac ggccaaaacg ccggagaatt ttcactccgg cgttttcatc
     8641 tgaaaacaaa ccagagattg cctgatgcgc tacgcttatc aggcctacct tttcctgcaa
     8701 cactttgaat ttatgagttt ttgtaggctg gataaggcgt ttacgccgca tccggcataa
     8761 aaaacgcgca ctttgtcaac aatctgaaac gccggaggtt tttctctccg gcgttatttc
     8821 ttacttcagc ataaagtcat actgaatact cattttcaca tcccagtaat tcaggcgatc
     8881 gcccagcgta ctgccatcgc gcttttcccg ccctttatcg gcagcaagaa agaaattcat
     8941 cccttgtaac ggcccttcgc tgacccgata gttcatacca cctaagatac ccgcgccttc
     9001 aatccgcaca tctcgtttac tggattcata gccgcgagtc acaatcggtg caaatgtcag
     9061 cgtaaattgc ggagttacac ggtagttgac acctaattgc caggactgca ttccttcacg
     9121 gttacgatca agacttaatg aaaagggata gccaatgtcg gtatccacca cggcaccatt
     9181 cattgcatgg cgcaaataac caccactgcc aaatacgctt ccccacggcg cattccaggt
     9241 caattgtcca ctaaccaacg ccgtttcatt gggctggctg gtattacgac ttaaaccttc
     9301 gagctgcgcg taaaagccga gcaactcccc tttcaacgta taattcgggt cgagcggtat
     9361 gcttttctcg agaatacttt gtatctgatt ggaaacatct ttctgtaccg atgcccccag
     9421 agaatagcga ctggtatcat catgccaact ggcggctaaa aaagaacgtg cgcgatgttt
     9481 atcgtatttt cccgtctggc tgttatacac cccttcatca cgaataccgg ttgttgaatc
     9541 agtccataaa aatactcgtg aacgccacat tccccacaga tcaatatttg gtgttaaagc
     9601 ccaataacct aatgctcctt cagaaactgt cggtaatgta cgttgcgaac tgccagccat
     9661 taacggaata cgataaggta agctaccata actataatcc gtcccgaagc gacctaattt
     9721 aagcaggcta ttttcactgg gttttatttt tactgccccc agggcaaagc caaaactttt
     9781 atcaccatca agataccacc ggatactcat atcagcacga gcacctaatt tataaacata
     9841 atacgctccg ccttcaacgc caataatatt ggaataataa ccactattga aatcgagcaa
     9901 tcctccctgt acccatgcgt aaatatctct tcccgcgcca gcactgggct tatcagccct
     9961 gcgcaattcg tttttcagtc tgatgcgtag tcccgcttca tcgtcagcca gttgcgccac
    10021 gtaagcagag gtaaggccag aggcagccgt caggcaaata acggccatgg ccactatttt
    10081 tctcatagtt gcacctttga tgttctgatt atttttattg aatattaatt agtgatatcg
    10141 ctgattaatt gctgctgcac ttttttacga ttatcaattt caaccacgat ttctttgaat
    10201 tttttatccg tgagcggata aaaccagata ataacgaatg ccagtagcat aaatccgcaa
    10261 ggtactaagg caattgatgt gcggatgccc ataataactt ccggcgttta cacctgattg
    10321 gcgatatatc cgcttaatcc caaaataaag gcaggaattg aacctccgat tgcctgaccg
    10381 catttacggg taaatgagaa tagtgaatag gtgagccctt caattcgcac gccggtcagg
    10441 tattcaccgt attctacggt atcagcttcc agtgcccaca tcacagtcat agtaacgccc
    10501 tgaccaattg aagcgatggc caacgcaacc aacgccaccg gcagtgacca gacggaaacc
    10561 cagaagaata gcagataacc gcaggttccc agcaaagcgc caatcaggaa ggtattcttt
    10621 ttaccgatcc tcgcgaccat ccccggcaca agcggtgccg atgccacagt accaaccagg
    10681 ttttgcacca gtaccagcac agtgaacagc ccggtatcat ttaacacata gcgcacgtag
    10741 aacaacgacg aggcgctgac cgcgaaggtc gaaatcagca cacacagcgc accgatgcac
    10801 aacataaaca gcgggcgatt ccgtttcagg gtttgcaaac tgatcttcaa tgacggctgc
    10861 gccacgatac gtaccacatt ttcacgcgtc gatttgaagc agatgaagta aagcaccatt
    10921 ccggcaatcg ccagcacaat cgtccagaaa tggtataccg acaccatctc ttccgggctg
    10981 gagttcttaa tgctcggtcc tatcagaaat gccaggcata caaaggtcaa tgaagcggca
    11041 atcccacgag ccgcgcccag acgggcgcga gattgtggtt gttgggtcat cgcggtagca
    11101 agtgaaccat aaggaatatt caccaggctg tagcaaagcc cgaggcccat gtaggtcaaa
    11161 tatgcataca ccactttgct accatggctc cagtcggtcg gtacccagaa taccagcacg
    11221 ctgaagatca ttaacggcgc agtaccgaag agtaaaaacg ggcggaattt tccccagcgg
    11281 gtattcacac tgtccaccac tcgtccggca aagacgtcgg cgaaggcatc aaatacccgc
    11341 accagtaaca gcatggtgcc tgccgcagcg gcaccgacgc cagcgacgtc ggtgtagtaa
    11401 ctcaatagga agagcgcccc cattgcgaag gcgaagttat tggcgacgtc accgaggctg
    11461 tagccgacga tggtgcgcca ggagagttgt tgattcattg tttgcctccc tgctgcggtt
    11521 tttcaccgaa gttcatgcca gtccagcgtt tttgcagcag aaaagccgcc gacttcggtt
    11581 tgcggtcgcg agtgaagatc cctttcttgt taccgccaac gcgcaatatg ccttgcgagg
    11641 tcgcaaaatc ggcgaaattc catacctgtt caccgacgac ggcgctgacg cgatcaaaga
    11701 cgcggtgata catatccagc catgcacact gatactcttc actccacatg tcggtgtaca
    11761 ttgagtgcag gccggctaac gtatccacgc cgtattcggt gatgataatc ggctgatgca
    11821 gtttctcctg ccaggccagc agttcttttt ccagtacctt ctctgccgtt tccaaatcgc
    11881 cgctttggac ataccaaccg taataacggt tcaggcacag cacatcaaag agatcgctga
    11941 tggtatcggt gtgagcgtcg cagaacatta cattgacgca ggtgatcgga cgcgtcgggt
    12001 cgagtttacg cgttgcttcc gccagtggcg cgaaatattc ccgtgcacct tgcggacggg
    12061 tatccggttc gttggcaata ctccacatca ccacgcttgg gtggtttttg tcacgcgcta
    12121 tcagctcttt aatcgcctgt aagtgcgcct gctgagtttc cccgttgaca gcctcttcgc
    12181 tgtacagttc tttcggcttg ttgcccgctt cgaaaccaat gcctaaagag aggttaaagc
    12241 cgacagctgc agtttcatca atcaccacga tgccatgttc atctgcccag tcgagcatct
    12301 cttcagcgta agggtaatgc gaggtacggt aggagttggc cccaatccag tccattaatg
    12361 cgtgatcgtg caccatcagc acgttatcga atcctttgcc tcgcaaatcc gcatcttcat
    12421 gacgaccaaa gccagtaaag tagaacggtt tgtggttaat caggaactgt tcgcccttca
    12481 ctgccactga ccggatgccg acgcgcagca ggtagatatc acactctgtc tggcttttgg
    12541 ctgtgacgca cagttcatag agataacctt cacccggttg ccagaggtgc ggattcacca
    12601 cttgcaaagt cccgctggtg ccttgtccag ttgcaaccac ctgttgatcc gcatcacgca
    12661 gttcaacgct gatatcacca ttggccacca cctgccagtc aacagacgcg tggttacagt
    12721 cttgcgcgac atgcgtcacc acggtgatat cgtccaccca ggtgttcggc gtggtgtaga
    12781 gcattacgct gcgatggatc ccggcatagt taaagaaacc atggaagtaa gactgctttt
    12841 tcttgccgtt ttcgtcggta atcaccattc ccggcgggat agtctgccag ttcagttcgt
    12901 tgttcacaca aacggtgata cgtacacttt tcccggcaat aacatacggc gtgacatcgg
    12961 cttcaaatgg cgtatagccg ccctgatgct ccatcacttc ctgattattg acccacactt
    13021 tgccgtaatg agtgaccgca tcgaaacgca gcacgatacg ctggcctgcc caacctttcg
    13081 gtataaagac ttcgcgctga taccagacgt tgcccacata attacgaata tctgcatcgg
    13141 cgaactgatc gttaaaactg cctggcactg caattgcccg gctttcttgt aacgcgcttt
    13201 cccaccaacg ctgatcaatt ccacagtttt cgcgatccag actgaatgcc cacaggccgt
    13261 cgagtttttt gatttcacgg gttggggttt ctacaggacg taacataagg gactcctcat
    13321 taagataaaa atactggtca acctttaatc tgattagtta accaattctg tgttgcacac
    13381 agttatctgt gagaaaccca tggaagagaa atggaaataa aaagaactga caccgcgatc
    13441 aaaaaaccaa aaactgcaat acccacagcg tgatatggat cgcattaatc tttaaaacag
    13501 cgttgacaga agccataaaa caagcaatcg agcgttcgtt cgagagtatt gggaacgata
    13561 aactctcatg acatatggct acggtgaata ttttgggggg gaattctccg aacacgggga
    13621 ggataatttt tgcacagaga aattacgata tgaaacgcgg gcacagttat caccagcccg
    13681 cgaaatgaga ggatcaggat gcggttaata taccgccaat catcgcgcgt aatccctgag
    13741 caaacgacgc ttcacgcgcc aggtcttccg cttcgatatc ggccagtaca ccgtaggtca
    13801 gatccagcag taaacgtgaa gtcattgccg tgttgatgtc tgggcttatc tcgccttttt
    13861 gctgggcatc acgcatccgc tgggcaacaa attcagtgat cgtcatatgc ttatttttaa
    13921 gcatggcggc gacctgtggg ttacgcatcc cttccgccat aatttcgacc accagcgccc
    13981 gttgcccaaa ggcttcatgg gtgagagaga caatagactc gaccatatag tcaacaaaat
    14041 gaatcccttc aatcggttcc cggaaacgag ccagcgccct ctcctggtcc tgtaagataa
    14101 tcgcctgaat caaggcttct ttggagatga aatggtgata gagcgtccca ggactaatgg
    14161 cgcaagattt acagatggct ttcatcgagg cactgtgaaa tccattttct gaaaaaatct
    14221 ctctggcagc attgaggatc cgggtccgtg tcggttgtgc ttcagtctgc atgttatcca
    14281 tcattaaacc attatagtta gcgtgaataa ctagtttacc gcgttgtcag gtgagcgcct
    14341 atcattgctg ttgaatagtg cgaaggtaca ctctattcgt atacttttca cccagcaatt
    14401 ggttaaccaa ttgccgtgat ttggctgcca gttgtttagc agagacgttg atgaattgca
    14461 cacgctatcc cggtaaacaa cacatacgac cgtttaccgg ttcgttagtg tattaattga
    14521 gctcctgtac cccaccaccg gagacggtga gaatttgtcc gcttacccag ctggctgcag
    14581 gcgagcaaag gaacagcgcc gcgttagcaa tatcttgcgg ttggcccaga cgtctgatcg
    14641 gcgtgtgctg taacattttt tgttcaattt ctgggtaatg actcca
//
LOCUS       DAJTXY010000099        14567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTXY010000099 DAJTXY010000000
VERSION     DAJTXY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.099"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            146..1186
                     /locus_tag="OO566_003049"
     CDS             146..1186
                     /locus_tag="OO566_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6606260.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1219..2412)
                     /gene="lplT"
                     /locus_tag="OO566_003050"
     CDS             complement(1219..2412)
                     /gene="lplT"
                     /locus_tag="OO566_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6606261.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2405..4564)
                     /gene="aas"
                     /locus_tag="OO566_003051"
     CDS             complement(2405..4564)
                     /gene="aas"
                     /locus_tag="OO566_003051"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6606262.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            4953..5984
                     /gene="galR"
                     /locus_tag="OO566_003052"
     CDS             4953..5984
                     /gene="galR"
                     /locus_tag="OO566_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6606263.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(5991..7253)
                     /gene="lysA"
                     /locus_tag="OO566_003053"
     CDS             complement(5991..7253)
                     /gene="lysA"
                     /locus_tag="OO566_003053"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6606264.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7375..8310
                     /gene="lysR"
                     /locus_tag="OO566_003054"
     CDS             7375..8310
                     /gene="lysR"
                     /locus_tag="OO566_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6606265.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8297..8989)
                     /gene="ygeA"
                     /locus_tag="OO566_003055"
     CDS             complement(8297..8989)
                     /gene="ygeA"
                     /locus_tag="OO566_003055"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6606266.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAS
                     IHAEDAVAFMLS"
     gene            complement(9118..10506)
                     /gene="araE"
                     /locus_tag="OO566_003056"
                     /pseudo
     CDS             complement(9118..10506)
                     /gene="araE"
                     /locus_tag="OO566_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(10821..11582)
                     /gene="kduD"
                     /locus_tag="OO566_003057"
     CDS             complement(10821..11582)
                     /gene="kduD"
                     /locus_tag="OO566_003057"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6606267.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11612..12448)
                     /gene="kduI"
                     /locus_tag="OO566_003058"
     CDS             complement(11612..12448)
                     /gene="kduI"
                     /locus_tag="OO566_003058"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6606268.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(12768..13916)
                     /locus_tag="OO566_003059"
     CDS             complement(12768..13916)
                     /locus_tag="OO566_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6606269.1"
                     /translation="MKDVVVVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            14171..14567
                     /locus_tag="OO566_003060"
                     /pseudo
     CDS             14171..14567
                     /locus_tag="OO566_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT         3779 a         3958 c         3449 g         3381 t
ORIGIN      
        1 ttaaccgtga tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct
       61 ggccttaaga tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg
      121 tcgtgattat aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga
      181 agtcagcacg ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc
      241 ccacgcacaa ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat
      301 gtacccagta cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg
      361 gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc
      421 gcgcaataat gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga
      481 agcgctgcat gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca
      541 ctggccgcag cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc
      601 gcctgcggtt tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa
      661 aattcgttat atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct
      721 ggcggacaaa cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa
      781 ccgcagtttt gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc
      841 ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc
      901 tggcgcacgt aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa
      961 agccgtcgcg gcgtatgttg atatcgccag acgtcatggc cttgatcctg ctcagatggc
     1021 gctcgcgttt gttcgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat
     1081 ggatcagctg aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc
     1141 tgaaattgaa gcggtacatc aggtttatac ttatcctgca ccataaagat taacgccggt
     1201 taaaaccggc gttaaatatt aatgacggcg ctgccagatc cacagcgccg ttattgccag
     1261 cgcaaatagc gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc
     1321 cagcgagtaa atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc
     1381 aatcgcattc cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg
     1441 aacgacaaaa aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcagcag
     1501 ctcgtgttgc agggagaaaa tcagtaccac cacgccaatc aaaatcccgg ctggcataca
     1561 gcgtgacacg gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc
     1621 aatcgctacc atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac
     1681 cggtacccac agcaccaaca ggaaacgcag cgtgacaccc gctccccaga ataaactggt
     1741 gcccaccagc gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg cattcaggaa
     1801 actgcgggtc atgttgatga gattccagga ctgccccgga cgcgccgccg ccagtttggg
     1861 aatgtagata ttggcaacga ccgcaccacc gtaggccagt gcgcatgcgg ccagggcgac
     1921 caggacatgc cagtcagcca gcacaccacc ggctacggaa ccgagtaaaa tcgccgctat
     1981 ggcagaagct tccattaaac cgttagcttt tactaactta ctacccgtgg ttaattcgcc
     2041 gagaataccg tatttcgccg gtgaataggc tgcagcacca acacccacca gcgtatagcc
     2101 gagaaacgga ttgataccaa agcagatact ggctgcgccc agcagcttca ggccgttggc
     2161 aaacatcatc acccggcctt tggcgaagct atccgccacc tgcccgacaa acggcgccaa
     2221 aagaatgtaa gcacctacaa acaccatttg caggatgggc tggctccact ccggatagaa
     2281 ctgcgctttc agtaacgcca gagtggcaaa cagtagcgcg ttatcgccaa acgcagagag
     2341 aaactgcgcc acgataaccg ctttcatccc cttcgaccac aacgaagtgt tagtgtgcac
     2401 tgactcactc atcgtgttgt tccgcttcgt ctacccagct tttcaacgtg acaaagtcag
     2461 gtttgccgct gccaagtaat ggcatctgtt tcagatagcg aatatcgcgc ggtacagcaa
     2521 gctccggcac gccgtgctcg cgggcatact gttgcaactt atcgcgcgtc agttcgttat
     2581 ctgtggtgaa aagcaccagt gcctcgcctt tgctggcatc gctcttaatc gcagtggcat
     2641 ggactttatc tggcgaaaca ccaagtgcca gttgttccac catttccagc gacaccattt
     2701 cgcctgcaat tttggcaaag cgttttgcgc ggccctgaat ctgcacaaag ccctgctcgt
     2761 caaaacgcac aatatcgcca gtgtcatacc agccgcgctc catttcgccg cgaacattct
     2821 cggcggtggg cacttccagt acacctggct tctccacccg cagatagccg ttcattatgt
     2881 tcggcccttt cagttgcagg cgtccgccct cttcgatacc agggaccgac aacaggcgtg
     2941 catccattcc tggtagaata cgccctaccg taccgggttt cgccgccatc ggtacgttga
     3001 tagaaacgac aggcgcgcat tcggtcacac cgtagccttc aaggatgcgc aggccaaatt
     3061 tatcctgcca aagctgtttg gtactttctt gtagtttttc tgcgcctgcc accacatagc
     3121 gtagacgata gaagtcatac gggttggcga aacgcgcgta gtgaccgagg aaagtcgagg
     3181 taccgaacaa cacggtgcaa ctgcggtcat acaccaagtc cggcacaatg cggtaatgca
     3241 gcgggcttgg ataaaggaac acttctgcac ctgtaagcag tggcgtaaac aggcctaccg
     3301 tcagcccaaa ggagtgaaac agcggtaatg ccgacataaa gcgatcgttg gtggtgaagt
     3361 cggcaatcgt tttaatctgc tcgacgttcg ccagaatgct tttatggcta tggacgacgc
     3421 cttttggatg gccttcagaa ccggaggtaa agaggatcag cgcctcttct tccggctgct
     3481 gttttacctg cgccaggcgc ggcatcagca aatgagcgaa gatccatact ttgtcggcag
     3541 tggtgacatc tgcttttaaa tcttccagat agacccagcg cacctgagta agttgctccg
     3601 gcagatgcca gagtttgcct ttatcgagaa actggcggga agtgaagatg gttttgattt
     3661 cagccgccgt aatagcactg gtcagccctt ttaccccggc agtgtagttc atcattgcgg
     3721 gaatgcggcg acgcgcgatg gccccaaaaa tcactgccgc actgatgcct gcattgggca
     3781 gcattaagcc gatgcgttcg ccttcaacac tgtatttttc aaggatgcgt ccaacaaaca
     3841 gcgttttcgt aagcaatttg cgataggagt ctggggtaaa gttgacgtct tcgacacatt
     3901 tcttcccggc tccgaagcgg tacattgcac tcagtaaaga ttcgtacaga gtttcacgcg
     3961 ggcgcaccgc catacgcgct tccatcatta tttgatgcag catttcgcca gcgattttgc
     4021 gacggtcacg ggcacgcggc gcatccggca tcgccacctg cgttggaggc aaaatatgca
     4081 gagtaatttg cgggaacaag cgacgtttaa ccagaccttt caggcggctg aagtgcgtaa
     4141 gttccgcccc ttcaatacgc acaggaataa ccgttgcacc agacttcgcc gcgacaaaac
     4201 ccgcgccatc gtagattttc atcagcgagc ctgtagtggt gatgcgtcct tcagggaaaa
     4261 tcaccactgg tcggccctgt tccaccagac gtaccagatg tttaatagcc ataggttgcg
     4321 tcgggtcgag aggaacaaag tcgataaatg atttcagcca acgcatatac cactgttggc
     4381 ttattgaggt gtaaacggca aacactggac gcacaggtaa aaacagtcca agcaaaatgc
     4441 catcaataaa agagacgtga ttaggcgtaa ttagaacgcg ctcgcccttc agtgcctggg
     4501 tgtcacccgt aacgcgaacg cgatacaaaa cacggcacaa atttcgaaaa aagctaaaaa
     4561 gcattccaac tccctttgct ctgattcagt aaaagcgaat ggagggagat tacacgagat
     4621 aaagaacgcg agcgacagta aattaggtgc gaaaaaaaac ctgcgcatcc gcgcaggttg
     4681 gtgcaagaga cagggtacga agagcgtacc gaataatctc accaatcaat acctctggga
     4741 tcaccacttt agcaacctga agccaaacgc caccagcggt caatcgcaac agcgtttcgc
     4801 aaagtgtaac caaaggtttg caatctcttt ttttgtctta cttatttgcg tttggctcac
     4861 attcccacga tgaaaacacg ccaccccttg aaccaacggg cgttttccgt aacactgaaa
     4921 caatgtaagc gtttacccac taaggtattt tcatggcgac cataaaggat gtagcccgac
     4981 tggcaggcgt ttcagtcgcc accgtttccc gcgtcattaa taattcaccc aaagccagcg
     5041 aagcttcccg gctggctgtg catagtgcaa tggagtctct tagctatcac ccgaacgcca
     5101 acgcccgtgc gctggcgcag cagaccactg aaacggtcgg tctgatcgtt ggtgatgttt
     5161 ccgatccgtt tttcggtgca atggtgaaag cggtcgaaca ggtggcttat cacaccggta
     5221 atttcttatt gattggcaac ggttaccaca acgaacaaaa agagcgtcag gctattgagc
     5281 aactgatccg ccatcgctgt gctgcgttgg tcgtccatgc caaaatgatc ccggatgccg
     5341 atttagcctc attaatgaaa caaatgcccg gtatggtgct gatcaaccgt atcctgcctg
     5401 gctttgaaaa ccgttgtatt gctcttgacg atcgttacgg tgcctggctg gcaacgcgtc
     5461 atttaattca gcaaggtcat acccgcattg gttatctgtg ctctaaccac tctatttctg
     5521 acgccgaaga tcgtctgcaa gggtattacg atgcccttgc tgaaagtggt attccggcca
     5581 atgatcggct ggtgacattt ggcgaaccag acgaaaacgg cggcgaacag gcaatgaccg
     5641 agcttttggg acgaggaaga aatttcactg cggtagcctg ttataacgat tcaatggcgg
     5701 cgggcgcgat gggcgttctc aatgataatg gtattgatgt accgggtgag atttcgttaa
     5761 ttggctttga tgatgtgctg gtgtcacgct atgtgcgtcc gcgcctgacc accgtgcgtt
     5821 acccaatcgt aacgatggcg acccaggctg ccgaactggc tttggcactg gcggataatc
     5881 gccctctccc ggaaattact aatgtattta gtccgacgct ggtacgtcgt cattcagtgt
     5941 caactccgtc gctggaggca agtcatcatg caaccagcga ctaaccgcag ttaaagcaat
     6001 tccagcgcca gtaattcttc gatggtctgg cgacggcgaa tcaaccgcgc ctgaccatta
     6061 tcaaacagaa cttctggtaa cagcggacgg ctattgtagt tggatgacat tgatgcgcca
     6121 tatgcccctg tatcatgcag taccagataa tcacctgcct tcacttccgg caaggcgcgg
     6181 gtttcaacat ttcccccttc ctgctgggta aagacatcgc ccgattcaca taacggcccg
     6241 gcgacgacgg tttccaccgt tggcgcgtgt tccagagaac gaccatcagc tgccagggca
     6301 ctgatatggt ggtaactacc gtacattgcc gggcgcatca gatcgttgaa cccggcatca
     6361 accagcacaa agtggcggct acccatttgt ttgacgctcc gcacctgagt aattaatacg
     6421 ccagactgcg ctaccaggaa gcgacccggt tcaatttcca gtttcacagg gtggcccaaa
     6481 tggcgagcga tttgctcacg cgcggcattc cacagaccat aataatgttc ggtatcaacc
     6541 gcctcttcac cctgttgata aggaatagaa agcccaccgc ccgcagaaat agcctgtaaa
     6601 tcctgaccga attcgatgac ctgacgcacc atagcaccac acacctgctc cagatgggca
     6661 taatcaacgc cagaaccaat gtgcatgtga atgccgacta gctgcagatg atgacgttgt
     6721 atcacgtcca gtgcggcggg cagatcggtg taccagatac cgtgcttgct gttttcgcca
     6781 ccggtattgg ttttttggct atgtccgtga ccaaaccccg gattaacgcg cagccatacc
     6841 cgatgccctg gcgaaacctg gcccagttgg tcgagcatat caacagaacc cgcattcacc
     6901 ggaatttgca attcactgac gcgttcaagc gtcgcctgat cgataacatc tgccgtaaaa
     6961 acaatatcat cggggtgcgt ttgcggattg taacccgccg ccaacgcacg ctctatttcg
     7021 cctaacgaga cggaatccac tttcacgccc tgctcacgca ttaagcgcaa aatatgaata
     7081 ttggaacagg ctttctgtgc aaagcgcacc acttcaaact gtttcagcgc tgctatctgc
     7141 cgacgaataa tttgcgcatc gtagacccac accgggcagc caaattcagc gggcaaacgc
     7201 agcagatttt cggcggtgag atcggtatcg gtgctgaaca gtgaatgtgg cataacaaac
     7261 tccagatgag tgctttttta tgattacgcc acatcataaa aagaataaaa aatatcgatt
     7321 tatgtcgaat ctatgcaaaa atgatatgga ttaccggatt gcgagagagc gctaatggcc
     7381 gccgttaact tacgtcatat tgaaattttt catgcggtaa tgaccgccgg aagcctgact
     7441 gaggcagcac acctgctaca cacctcacag ccaaccgtca gccgcgaact ggcgcgcttt
     7501 gagaaggtga tcgggctgaa attgtttgag cgcgtacgtg gacgattaca tcctaccgtg
     7561 caaggactgc gtctgtttga agaagtgcaa cgatcctggt acggactgga tcgcattgtc
     7621 agcgccgcag aaagtctgcg cgagtttcgc cagggagaac tgtctattgc ctgcctgccg
     7681 gtcttttcgc aatctttttt accgcagctc ctgcaaccct ttctggcacg ttatcccgat
     7741 gtcagcttaa atatcgtgcc ccaggaatca ccgctacttg aagagtggct ctcggcccag
     7801 cgtcatgatt taggactcac tgagacactc catacgcctg cgggaacaga acgtaccgaa
     7861 ttactctctt tagatgaagt gtgtgtgtta cctccgggtc atccgctggc ggtaaaaaag
     7921 gtattaacgc cggatgattt tcagggtgag aattacatca gcctttcccg tactgacagc
     7981 tatcgccagt tgctggatca gctatttact gaacatcagg ttaaacgacg catgatcgta
     8041 gaaacccaca gcgccgcgtc agtctgcgca atggtacggg cgggggtagg tgtttcggtg
     8101 gttaacccgc tcaccgcact agattatgcg gcaagcggtt tagtggtgcg gcggttcagt
     8161 attgcggttc cgttcaccgt cagcctgatc cgccccctgc accgcccgtc atcagcgctg
     8221 gttcaggcgt ttagtgggca tttacaagcg gggttaccga aactggtcac ttctcttgac
     8281 gctattttgt cgtcagctac gacagcataa aagcgacagc atcctcggca tggatcgacg
     8341 cggtatcaaa cacaggcaaa acactgcgct cttctggcac cagtaaacca atttctgtgc
     8401 agccaaaaat gacgccctgt gcgccctgtt ctgcaaggcg agcaatcact tgcgcataat
     8461 aagcgcgtga cgcttcggta aatttcccca gacacagttc ttcaaaaata atctgattaa
     8521 ttttcgcccg ttcatccgct tcaggaataa gacaattgat ggaaaattgt tccgtcagcc
     8581 gcccgcgata aaaatcctgt tccatggtgt aacgcgtacc cagcagcgcc acacgagtca
     8641 ttccggcccc ggtaattgca cgtccggtgg catccgcaat gtgtaaaaaa ggcagagtgc
     8701 aacgtgactc aatggcatcc gccactttat gcatcgtatt ggtacataac acaatacctt
     8761 ctgcgcccgc ccgctgtaag ccaagcgccg cctcagccag aatgtccccg gttttatccc
     8821 attccccgcg acgctggcac tcttctattt catgaaaatc gacgctatgt agcagcattt
     8881 gcgcagagtg aagcccacca agccgctgtt taatgccttc atttatcaga cgatagtaag
     8941 gaatggtgga ttcccagctc attcctccca gcaaaccaat tgttttcatt cctctctcct
     9001 gatgtgtgtt accgcaacaa ggctgaataa ggaacgagac aaacgcctca acggccaggt
     9061 gcccaatctc tattaacgaa aaaagggccg gatgtacagc acatccggcc cgtgaaatca
     9121 gacgccgata tttctcaact tctcgcctgc catcagtttg cgttcgatat gttccagcgt
     9181 gacatttttg gtttccggaa tgagccagaa agtaatgccc acaaacgcaa tgttcaacgc
     9241 agtgtagagc cagaacgtac cggcagcgcc aatgctatca agcagtgtca ggaaggccgc
     9301 gccgataatc atattcgaca cccagtttgt cgtcgtcgaa caggtaatac cgaaatcgcg
     9361 gcatttcagc ggctgaattt cagagcacag gatccacacc actggcgcgg cgctcatcgc
     9421 ataaccggca atacacatca tcgtcatgcc aacagagagc caggacaagc cactggaagc
     9481 cgtaccgtta tcaaactgca tcaggcaata gcccagcacc agagtgccta acgccatcac
     9541 gctgaaacca attttcagag ccggtttacg acctgcttta tctaccgtaa agaccgcaat
     9601 gaaggtcgcg aacataaagg tcagtcccac gaccagagtc gcaatcatct gttgttctgt
     9661 ggtcgtaaag cccgccattt tgaagatacg cggcgcgtag tacatgatga tgttcatacc
     9721 ggtaaactgc tgcatcgcct gcaacaacat accgagaaac acagcacgac ggacgttacg
     9781 gttgatctta aacagtgccc aaccgccctg ttttaacttc aggctttcac gaatttcgtt
     9841 gagttcttct cgcgcttttt ccgacgtatc gcgcagcata cgcaacactt cttccgcctc
     9901 aatatgacgc cccttttccg ccagccagcg cgggctattt ggcaggaaga ccaccagaat
     9961 aatcagcaga actgctggta aagcaagaac cccaacattg cgcgccagtt accgctataa
    10021 ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat caactggtac
    10081 atactgatca tcttaccgcg aacgtacttg ccatttcaga aagatacaga ggagcggtgt
    10141 aagacgcgat cccgacagca atgcccagca ccacacgagc ggcgattaac atctctacgc
    10201 tggtcgcaaa agcggaccct atagaaccga gtacaaacag gatggcccca ggcggaacga
    10261 cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac taaccaccca
    10321 ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg ctccggcgat
    10381 tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg aaacaaacat
    10441 attcatacgc cgcgtatcac gcaaagaacg tggcgttaaa gcagattccg tattgatagt
    10501 aaccattttt tcctgccagc agagagtaag acattgtgaa aaaatacgtg aacaattcac
    10561 acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca tgccgttatg
    10621 tgatggatat tccaatttta aaattaagtt gaattattga gattattatt aaccacctaa
    10681 ttttaaagta gataaaattc ataaagttca ttaattgata attgatatgg attatttcat
    10741 aaccatgata tggattatga tgatctagag atataaaaaa ccctgccatg cggcagggtc
    10801 ataaaagtaa gaggaatgaa ttaacgcgcc agccaaccgc catccacagc aatggtataa
    10861 ccatttacat aatctgaagc gctggaggca agaaacacta ccggccccat caggtcactc
    10921 ggcagtcccc aacggccagc tggaatgcga tcgagaattt ccgcgctacg ttgttcatct
    10981 gcccgcagtt gttgggtatt gttggtcgcc atgtaacccg gagctatcgc attaacatta
    11041 atgttgtgtt tagcccattc gtttgccatc aatcgcgtca cacccatcac tccgcttttt
    11101 gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga cgcgatattg
    11161 ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg agacatgaag
    11221 aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct gaactcgaga
    11281 gcgtcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg accaaattca
    11341 gctaccgcgc gatccagcag tccaggaatg ccatcaatct ttcgcagatc ggcggtcagg
    11401 cttaaaaaac gacgccccag cgccgtgacc tgctcgatgg tttcagtcgg ttcaacgatg
    11461 ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc ctggcccagc
    11521 ccagtatcac aaccagtgac gaccgcaact ttaccttgga gagaaaatgc acttaaaatc
    11581 ataacaacac cttattcgtt tatgcccaca actagcgcaa atctttaacg gccacatggt
    11641 ccatatcatt aaagacctgg ttttcaccga ccatgcccca gataaaggta taagctttgg
    11701 ttccgacgcc ggaatggatc gaccagctcg gggagatcac cgcctgctcg ttatgcatca
    11761 caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg tcatcatcca
    11821 tattgaaata gaaataaact tccatccggc gctcgtgggt gtgacacggc atggtgttcc
    11881 acaagttacc tggagccagc tccgtcagcc ccatactcaa ttgacaggtt tccagtacat
    11941 ccgggacaaa atatttgtta atcgtgcgac ggttactggt gaggttatcg cctaacgtga
    12001 ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc gcgggtgcgc
    12061 aattgtaata aaacttcgcc ggagtggcgg tatcaatact ggcaaagaca acttcttttg
    12121 cacctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattga ccatcgacag
    12181 taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca aggaaatagc
    12241 ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt atcggcataa
    12301 tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca tcggcgacaa
    12361 ataccttttc aaccaaaaat tcattacgca gcccttgggt atccagcgtt tttgcgtgcg
    12421 caccgtggat gctctgtctt acgtccacat caacctccga taatcagtga agcgtgaaaa
    12481 tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata tgcccatggg
    12541 ctagggtttt caattaaaaa taaaacaatg ttttattttt aatttaagga ttaaaaaaag
    12601 tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc agaaaatgta
    12661 tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt tatcggtaag
    12721 gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgcccta gcccccgccg
    12781 atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg aaccagagaa
    12841 accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc gccaccattg
    12901 acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc ctgtgcagca
    12961 aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac acgctccagg
    13021 caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac accaacgctg
    13081 gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt ggcttcgctc
    13141 atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc ggtcacagaa
    13201 ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc gtcagtgcgt
    13261 ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat taccgggacg
    13321 atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg cgagctaagt
    13381 gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt ttcggcggtg
    13441 acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag actgtcaacc
    13501 aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg tggtgcgcgg
    13561 ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg tatcgcctga
    13621 gtagccagat gcagtgcttt aagaccggaa ccgcaaacgt cattaatcgt gattgcagaa
    13681 acgctgttag gaagaccacc tttaatagcc gattgccttg ccggattctg ccctgcccct
    13741 gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac gccggtacgt
    13801 tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc cgctaacgca
    13861 ccacgaaagc agccgatagg tgtccgtaac gccccgacaa ccacaacgtc tttcatcaca
    13921 acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtta tcttaattgt
    13981 ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca gagtcataac
    14041 tggcacgtaa ggtttgcaac cactaaccca ccaatagatg ggtagatagg gcgttaatct
    14101 cccatactta acttggttta tggtaaattg ccctccattt tgtttaattt gtagatgata
    14161 cgttcagata atgtctaata tttggtctaa agaagaaact ctgtggagtt tcgcgctcta
    14221 cggcacagcc gttggtgcag gcacgctatt ccttcctatt cagttaggtt cggcaggggc
    14281 tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattagc cacataaagc
    14341 cttatgccag ttcatcctct catcaaaaac atcagcaggt gaagggataa cgggcgcggt
    14401 aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact tcatcgcctt
    14461 ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg aacagctggc
    14521 aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgg
//
LOCUS       DAJTXY010000100        14314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTXY010000100 DAJTXY010000000
VERSION     DAJTXY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.100"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(337..582)
                     /locus_tag="OO566_003061"
     CDS             complement(337..582)
                     /locus_tag="OO566_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606270.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="OO566_003062"
     tRNA            complement(1227..1302)
                     /locus_tag="OO566_003062"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="OO566_003063"
     CDS             complement(1408..2115)
                     /locus_tag="OO566_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6606271.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="OO566_003064"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="OO566_003064"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6606272.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LVIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVVMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OO566_003065"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="OO566_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6606273.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OO566_003066"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="OO566_003066"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6606274.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="OO566_003067"
     CDS             complement(7299..7574)
                     /locus_tag="OO566_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6606275.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OO566_003068"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="OO566_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6606276.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQMLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPKFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="OO566_003069"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="OO566_003069"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6606277.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="OO566_003070"
     CDS             9534..9860
                     /locus_tag="OO566_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6606278.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="OO566_003071"
     CDS             10044..10763
                     /locus_tag="OO566_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6606279.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="OO566_003072"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="OO566_003072"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6606280.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13109
                     /locus_tag="OO566_003073"
     CDS             12102..13109
                     /locus_tag="OO566_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6606281.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13165..14314)
                     /locus_tag="OO566_003074"
                     /pseudo
     CDS             complement(13165..14314)
                     /locus_tag="OO566_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
BASE COUNT         3730 a         3430 c         3689 g         3465 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgccggttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aagtagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctggtgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctctg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggtga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac gtcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agtagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgattcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaacttcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcatctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agtacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tgcccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacggcg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaattactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat acaaagctaa gggataatgc gtagcgttca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgacta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13021 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctggaaaaaa gataccgaac
    13081 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13141 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13201 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatggcag
    13261 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13321 actacccacc ggcagtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13381 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13441 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13501 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13561 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13621 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13681 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13741 ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa
    13801 ggcatccaga aacgctttac aggcctgagc aaaggttgct ctttctgaaa gggggtagcc
    13861 acggcgttta gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg
    13921 caaatatccc gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac
    13981 aattaacacg tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc
    14041 ggtagaaaat gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac
    14101 ggcagcagct gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac
    14161 gttcacatga ccaagcgaac caggaacgcg gccaaccatt acctaggcga ggttaatcaa
    14221 acgtatcgca ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc
    14281 caacagggca aagttatcca gtccattagc cagt
//
LOCUS       DAJTXY010000101        14242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTXY010000101 DAJTXY010000000
VERSION     DAJTXY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.101"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..96)
                     /locus_tag="OO566_003075"
     CDS             complement(<1..96)
                     /locus_tag="OO566_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR6606282.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNH"
     gene            complement(137..592)
                     /gene="csgB"
                     /locus_tag="OO566_003076"
     CDS             complement(137..592)
                     /gene="csgB"
                     /locus_tag="OO566_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6606283.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1346..1996
                     /gene="csgD"
                     /locus_tag="OO566_003077"
     CDS             1346..1996
                     /gene="csgD"
                     /locus_tag="OO566_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6606284.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2001..2390
                     /gene="csgE"
                     /locus_tag="OO566_003078"
     CDS             2001..2390
                     /gene="csgE"
                     /locus_tag="OO566_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6606285.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            2415..2831
                     /gene="csgF"
                     /locus_tag="OO566_003079"
     CDS             2415..2831
                     /gene="csgF"
                     /locus_tag="OO566_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6606286.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSVLQNNSTDF"
     gene            2858..3633
                     /locus_tag="OO566_003080"
                     /pseudo
     CDS             2858..3633
                     /locus_tag="OO566_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3697..4188)
                     /locus_tag="OO566_003081"
     CDS             complement(3697..4188)
                     /locus_tag="OO566_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6606287.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4290..4844)
                     /gene="ycdY"
                     /locus_tag="OO566_003082"
     CDS             complement(4290..4844)
                     /gene="ycdY"
                     /locus_tag="OO566_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6606288.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4868..5605)
                     /gene="ycdX"
                     /locus_tag="OO566_003083"
     CDS             complement(4868..5605)
                     /gene="ycdX"
                     /locus_tag="OO566_003083"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6606289.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5660..6598)
                     /gene="ghrA"
                     /locus_tag="OO566_003084"
                     /pseudo
     CDS             complement(5660..6598)
                     /gene="ghrA"
                     /locus_tag="OO566_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6832..6919
                     /locus_tag="OO566_003085"
     tRNA            6832..6919
                     /locus_tag="OO566_003085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6866..6868,aa:Ser,seq:gga)
     gene            complement(7069..7350)
                     /locus_tag="OO566_003086"
                     /pseudo
     CDS             complement(7069..7350)
                     /locus_tag="OO566_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7632..8636)
                     /locus_tag="OO566_003087"
     CDS             complement(7632..8636)
                     /locus_tag="OO566_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR6606290.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8708..9268)
                     /locus_tag="OO566_003088"
                     /pseudo
     CDS             complement(8708..9268)
                     /locus_tag="OO566_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9353..9550)
                     /locus_tag="OO566_003089"
     CDS             complement(9353..9550)
                     /locus_tag="OO566_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606291.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9562..10053)
                     /locus_tag="OO566_003090"
     CDS             complement(9562..10053)
                     /locus_tag="OO566_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606292.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10050..10424)
                     /locus_tag="OO566_003091"
     CDS             complement(10050..10424)
                     /locus_tag="OO566_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6606293.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10471..10845)
                     /locus_tag="OO566_003092"
     CDS             complement(10471..10845)
                     /locus_tag="OO566_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6606294.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11008..11229)
                     /locus_tag="OO566_003093"
     CDS             complement(11008..11229)
                     /locus_tag="OO566_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6606295.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11292..11768)
                     /locus_tag="OO566_003094"
     CDS             complement(11292..11768)
                     /locus_tag="OO566_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6606296.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11784..12257)
                     /locus_tag="OO566_003095"
     CDS             complement(11784..12257)
                     /locus_tag="OO566_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6606297.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12599..13420)
                     /locus_tag="OO566_003096"
     CDS             complement(12599..13420)
                     /locus_tag="OO566_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6606298.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13520..13693)
                     /locus_tag="OO566_003097"
     CDS             complement(13520..13693)
                     /locus_tag="OO566_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6606299.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13813..>14242)
                     /locus_tag="OO566_003098"
     CDS             complement(13813..>14242)
                     /locus_tag="OO566_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606300.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3639 a         3487 c         3408 g         3708 t
ORIGIN      
        1 gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac cggagaatac
       61 gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc ggattgattt
      121 aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac tgtctctgca
      181 ctacaattgc cgttttttga gtaccatact gtgtaatatt tgctttatta ccagaacctt
      241 tctggataat catcgcagta ttaccataag caccttgcga aatactggca tcgttggcac
      301 tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc tttgcccgat
      361 tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc cgtaactgag
      421 cactattatt agtcccagct tgaccaatta tggctgcctg attaaatgaa gacttactca
      481 attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct gcggctgcaa
      541 tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc atgttgtcac
      601 cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt taacgcaacc
      661 tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat ttttaataac
      721 tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa cccgcgtaaa
      781 tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt ttccgctatc
      841 aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata tgtcttgcgc
      901 acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga ggtgtggaga
      961 aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt atatgtatta
     1021 ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa ccaaatgtac
     1081 aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta atataatgag
     1141 ttacattttg ttacatattt aacacttgat ttaagatttg taatggctag attgaaatca
     1201 gatgtaatcc attagtttta tattttaccc atttagggct gatttattac tacacacagc
     1261 agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt caggtgtgcg
     1321 atcaataaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc atggtcatac
     1381 attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc accttaaaca
     1441 atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg atatctcttc
     1501 aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc attattggca
     1561 ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata cgcctgaaga
     1621 ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt atgccatgga
     1681 ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat gctactttac
     1741 gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata acagcacgga
     1801 atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta tcggcgcgtc
     1861 taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa cgcatcttta
     1921 taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct gggcaaacga
     1981 taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg gcagaatttc
     2041 tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg ttaactgacc
     2101 atactgtttc atctattggc catgattttt accgagcctt tagtgataaa tgggaaagtg
     2161 actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga agctggatca
     2221 caataacggt caatcaggat gttattttcc agactttttt attcccgttg aaaagagact
     2281 tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat cgtcgccaga
     2341 taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa ataaaaaatt
     2401 gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt atttcgccat
     2461 taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt ggtaacccaa
     2521 ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa gatccgagct
     2581 ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact caggccatcc
     2641 agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg ggccgcatgg
     2701 tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag ttgaacgtga
     2761 cagatcgtaa aaccggacaa acctcgacca tccaggtttc ggttttacaa aataactcaa
     2821 ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat ttcttttggt
     2881 tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg ccagaccgac
     2941 attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc cgacgggtaa
     3001 gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac cctacccggc
     3061 aagtaacttc tccactgctg ttccgcaaag cgccagggct tacaaaacct gcttaacgag
     3121 cgcaaggtta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa ccgaatcccg
     3181 ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattattgg ttatgaaagc
     3241 aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga cacgcaatac
     3301 cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg tgagatcctc
     3361 tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg ggttttccgc
     3421 tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa cgaacccgtt
     3481 atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa tgatggtatc
     3541 gaccgtggtc tgtgggattt gcaaaataaa gcagaacggc agaatgacat tctggtgaaa
     3601 taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga attaataatc
     3661 cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt tgatttcctg
     3721 ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt ttttcattgc
     3781 gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt caccctgccc
     3841 ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca gcagctgttt
     3901 ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga gaatttccag
     3961 atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc cactaagcag
     4021 ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa agtaggccgt
     4081 acagcccagg aagcccgccc agcttagtaa acccagggaa acagccaccc atccccagat
     4141 accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc cgcgcatgat
     4201 agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa caaattatct
     4261 gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc cagctcgtcc
     4321 cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa aggcgtggtt
     4381 gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata ctcactgaac
     4441 agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca ggaagccgca
     4501 agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtggcatccc tcgttcggaa
     4561 agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg atatggcggc
     4621 acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg cgtcatatca
     4681 cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt cgcagccagt
     4741 ttcccctcac gaatcagagt aaacagcggc accagtaaag gatcttgcgg ttggcggtaa
     4801 tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat atttccatta
     4861 ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat tcaaggaagt
     4921 tcagtaagcg gcgcggagaa acattcaaaa tgcgctctgg cggaaaatct accgcgtcga
     4981 ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa tccgagccta
     5041 atgccaccca acctccagca tcacgtaccg ctgcagctac cgcacgacag ttgtcttcac
     5101 tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc tgatgtttcg
     5161 cggctgcttc agcaacggct ttaacatcta tttcatattt cggatttccg ggatggctta
     5221 ttatatgcac attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg gtcgctttgt
     5281 catgtggcgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc gagtcaaaca
     5341 ttttaccgct gcagtcaatt tcaccatcaa catttttaat gttagcttca atgccgcgca
     5401 ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag tgccagtgat
     5461 gcggcgcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta atgccctttt
     5521 gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg gcaacggtat
     5581 gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg ccaaggatag
     5641 caggaatcct gatgctttat tagtagccgc gtgcgcggtc gacttgcccg cagaccctct
     5701 cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca gcgggacggg
     5761 taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc ggactttcag
     5821 gcggtaaggg ttcacgatta aaaacatcca gcattgcgcc tttaactttc ccgctatcca
     5881 gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg aggagatacg
     5941 cgccatcagg taatttttcg agtaattgtt gattaataat gccgacggtt tcaggggtat
     6001 tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt tcttcccgtc
     6061 cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa cgcagcggaa
     6121 agcgctaggt ttgcagactc tgagcaactt tacagcccag tacgcctgcg cccaaaatgc
     6181 cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa ctattttgct
     6241 ggatgcgata atcatcaaaa cgtcgaaacc aatgcagcac ctgactgaca gcatattcct
     6301 gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa gggttcagca
     6361 tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg agtgcgaaca
     6421 ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact aaaacataat
     6481 cagcagagtc attatctccg cttttccatg ccctgactct tgcctgagga atagctttgc
     6541 gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg atatccatac
     6601 tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat ctacaaaggc
     6661 caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt attaaaacca
     6721 cacattgatt gaagtttgaa taaacgcgcg attctttcaa aaagtttgtt gacctcaggt
     6781 catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt tggtgaggtg
     6841 tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat cgggggttcg
     6901 aatccccccc tcaccgccag attataagtg catgttattg tttaacatgc gcttttttat
     6961 ttttccttcc agacagtatg ccatcgggta tgccatctga aaccggttta tatgccccgc
     7021 gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct atactgccag
     7081 catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt ctggttttct
     7141 gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct catcttcata
     7201 gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga tgtttccccg
     7261 gttgtcgggg attggtttac cgctgaacag gtgcaacatt ctttccagat cggcgagtcg
     7321 gtcagcctgt tgtccggtga taagatgaaa tctatggtca cccccgtttt tgcaacactg
     7381 atttttgatt aatggttggc ttgcttaaat ctatccggcg tctctgtggg caaggatgcc
     7441 cgcgccccga tgagttccgc acctgataat cctaaaaatc ccctcggctt cagtgagcca
     7501 tttcatctgt taggttttta atcaggtcga tttgccgttc atgtcatcaa taatcagtct
     7561 tcgcaaaacc agaaaggtaa cttgtatctt attgtttaaa ataaactaat tgagttatat
     7621 cagcatctat atcaggcctg atattctttt ttctgagtag tgattgccca ggcaattcga
     7681 gccagtttat ttgccagagc gcaggtaacc acattcgaat gcttcttgtt gagttgctcc
     7741 cgaacccact cagcaagttt tccatgcttg cgttcaagtc gcattatgaa tgagcgggca
     7801 cactgaacca gtaaccggcg taaattttta tcacctcgct tactgatacc caacagagtc
     7861 gattttcctc cagtgctata ctgccgggga acaagtcctg tggaagctgc aaaatccctg
     7921 ctgcatgaga actgtttccc gtcaccaagt tgcgacgata gtaagctggc agtaatcgga
     7981 ccaacaccag gaattgtcat aatgcgctga gcggtatcat cagcgttgat tgactgactt
     8041 aattctgatt ccagctctgc tatttgctca acaaggtaaa agtagtgggt atgcaacctc
     8101 accaaaaggc ggctcagata ttcggggatt tcgtgttcag ccaacacgag agacaaccgt
     8161 tttattacag catcacctat cggcaggctg attccaaatt ccagcaagaa accgtggatc
     8221 tggttggttg ttttcacctt atctctgatg agcgattctc tgacccggtg taacgccctc
     8281 atggcctgct gggtctcagt tcttggctgg acaaaacgca ttgatggtcg tgaagccgct
     8341 tcgcatatgg cttctgcatc aacaaaatca ttcttattac ttttgacaaa cgggcgaacg
     8401 aactgtggag aaatcagctt agtttcatgc ccaaaatcag agatacagcg agccataaag
     8461 tgtgcacccg cacaggcttc cattacaaca actgagggcg gacatgtcgc cagaaactgc
     8521 atcagtttag tacgggtaaa ttttttacgc agaagagcct taccagactt gtcttgacaa
     8581 tgaatgtgga aggagtgctt tccgagatcg ataccaataa gtgtgatgtt ctgcatgatg
     8641 gtctctccgt taaagggatt taccttaaca gcgtactgct gttaaggtgg gggtgaccat
     8701 atcattatcc ccaccggtcc catctcacca gattgttgac gataatttta ctgccaaatt
     8761 tacaggggca attggttttg taattccagt tcagccctct gaagacgttg atcacccctc
     8821 gctcaaacac ctcatcctta ttctggtgca gttgttcaaa tgtgctcaga atgttcgctt
     8881 cgctgatttc cggaaaatta tcgtactcca gtgaccggta ccatgtatca cgggcctgcg
     8941 tatccattaa tgacagcatc ccggaacgtt gcatcaggtc acgccagatt tcgcgatcaa
     9001 gattccgggt aatggctttc attgctgttt cgggtttctc catcagccag cagccgcagc
     9061 gaaaatcctg tttcatggcc cagtccgggg cagttttacc tccgatgctt tccgtcagca
     9121 ccgagatttc agccagttgt cggataagtg tgtcgatttg caacagtgta ctctttcgta
     9181 cagccagaat gtggcgaatg ctggttgagc agataacgtc cgtatgcccg ttgaccactt
     9241 ccggacggga gattgtgata tctgacataa tcgatattcc tttttgcaga taaatgaaaa
     9301 cgccagccct gtgcaggctg gcgtcagagg aatgaagtct ggtgagtgag cttcaccggt
     9361 tctggtgcag aagctgcaga tgacgcaggg tacggacatc ctcccgggcc tgttcgatac
     9421 agggcaacag tgctgctgaa tccgttttat cctcatcgtt gtcaaagata attcccgatc
     9481 cgcagtcgat attgtcctgc agccacgcaa tcagaatatc cagcgctgtt tccgtggtta
     9541 atgatttcat gttgttcctg gttaccagtc gaaagtgggt aaacccggca ggcattcagc
     9601 gctggcatcc agatgaatga gactgacgcc ataacgccgg atgagtgtga cgaccagacg
     9661 acggaacgtt tttgacagcc ccaggcgttt aagccgcaga accgggtatg accacgcatc
     9721 ggtacgtaac agatatccgc tacctgtaaa atgaatccat tctgattcac cgaagtcact
     9781 ggtctggtgt gacagcgagt acaaccaggc gttgtccgtt tccgtgatat gtgcggtact
     9841 gcagcgtatg ccggtaaggg tagcaaatgg tggcgtgcat tctgctgagg tctggtcaga
     9901 ttcatcaaca acacgaaggg agtagccgtt atcgcagacc atgttaatca gttcagcgag
     9961 ctcaatacca tcgacgtcaa cgacaatgcg ccctatgttc agtgcctgta cgttaacgct
    10021 gtcggcttcc agtgtcaggg ccagtttcat catttcgcct ccggatactt acccagggta
    10081 atgttattta ccgttctgta attgtcgcgg gtcatcaggc cggtcgcccg gcgagcccgg
    10141 aggatatcaa tactgtttat taactgagag cggggacagg tgctgaatcc cggctggtcg
    10201 gtacgcacca gcgcgtattt ttccacgaga aaattaactg catcacacag tgaaatgcct
    10261 gcctcaatat gctgctcaat cacacgttca tcgacaaacg gtgtgtcatt cagtgtgagg
    10321 ccataatgct ggtccagcag tcggatgagc agtgtctgcc agatggtgac gggagacggg
    10381 cagcgagata cctcccgtac atgtgtgtcg gataatgttt tcatcttaag ttctctgtaa
    10441 ctggtagtgc taaaggtgag agcttcgggg ttatgaggtg gttgcgggtg ctggtgtcgg
    10501 ataaacagcc agataaacgt aaccacagga gccgagggtg tcggcttcgc aggtcagccc
    10561 ttttgcatac agcgtgacgg tatgctgata tcggggattc agttcaccgc tggtgagcat
    10621 gagttccagt tgtttcatca gcagcggaaa ggcctggtcc agatggtatg catccgcatt
    10681 gctgaaccgg cctctgatac cggcgcggtc tgcaaggtaa tacaggcggt taccctcttg
    10741 caccagacga gcgccaaaac agggcctcac tgtgcaggga agcccccacc aggggcggtc
    10801 gtgattgtca tcgggatgag ttatcccgga gagtttgtct gacacgataa aatcctctct
    10861 gaaaagtggt taagaatggt ttaacgatga caggtcagcc ggcgcgctga ccggtttcgg
    10921 tgaagatgta tgcattgatg agaatggcct catccacagc ggcgtctgag gtcatctggt
    10981 cgtactcgtt tcccgggtga cggaacatca gttcagggtg acactcatca gccggacata
    11041 cgggccaaaa ctgtccttac ggcgttctgc aaacacggca agaacgccgg gaatatcctg
    11101 cacttcacga ccagtatacg cttcagcact gccgtgccag cgatacttac cggtacagaa
    11161 cggaaaaaga cgggatgcag gatgctgttg gtgaatacgc atggcttcac cacgggtgat
    11221 gattctcatg atgtgatacc tctgaagaca gaagataaaa gtgaaaacag gtgtgatgtg
    11281 gttgtgacgg gttaaagcag accatgttcg gcaaaggaga aaacctggtt gccaccgact
    11341 atcagatggt ccggcacccg gatatccacc aggcccagtg cctgtaccag acgttcggtg
    11401 ataagccggt ctgccttact gggtgtgact tcaccggacg ggtgattgtg cgccagcacc
    11461 acggcagcgg cattgtggta cagggcgcgt ttaatcactt cccggggatg aacttccgta
    11521 cggttgatgg tgccggtgaa gagggtttca ccggcaatca gctgattctg gttgttcaga
    11581 tacagcaccc ggaactcttc ccgctccagt cccgccatgt tcagaatcag ccattcccgt
    11641 gccgcacggg tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc cagggtttgc
    11701 agggcccgca gaatgagact gcgctcgccg ggcgtcatgt ttccgggcag aaaggaaagc
    11761 tgctgcattg tccctgcctc cattcagtcg atgatgcgca taatggcgct gcattccgga
    11821 tgctgcaggg cgtaatcccg cagccggtaa taatggaccg tcatggcata acactccgta
    11881 cgacaggcat gatgactgta cgtcatcaga caggcagcaa tgccggcggc ttccgggctc
    11941 atttcagcgc ggttaccgtt catggcattg aacagtaccc atgtttcgtc atcatcgtca
    12001 tccggttcgg gtgccataaa tgccccgccg ttgttcaggg tgtacagatt ccagatacca
    12061 ccgcagtagt cttcacacag acggtccatc cagccgaaga cacggggttc cagggtgacc
    12121 cactgtggaa tgaggccaaa atgctgcggc cagaagctga tgcgctgttc atcagggacg
    12181 ggagtggcaa ccagttgttg caggccagtc tccggtgcac aggccgaagc ggtggtgttc
    12241 tgagacaatg ttttcatgta tgaaatcctt gtaatgtgaa taaatgagag ggagtaaagg
    12301 tcgggagctg gcaacatcac gttcatcact gaccgtgatg acccggtaat atcagggatg
    12361 aatgatattc ggctgaaatt ccggacagcc gtacgcgtaa ccgttccggt cacagacgga
    12421 tggccagatg ccattgcctg tatactcagt ctgcacagac tgatttctcc gggacggtaa
    12481 agcagacggg tacaccaggc gcacaggtca tcatcagaaa tggaacgaca ccccggcagc
    12541 gcctccgggg cgtcgggtga ttctggtgcc tgaatcacgc cttcagggac atgctgcctc
    12601 agcgcaggct ctccagcagc gtttctgcca ttacccacaa tgcgcggttg agcttaatat
    12661 cggtgtcgat gttgtgaatg gcacgggtgt ggatacgttt tccttttgca ctgcggccgg
    12721 aaatcccgcc tttcagcata ttctcctgga tggtctgata tgcgctccac aggtctttac
    12781 cgtaatcctc cctgcgtcgt ggtgtcagaa tgtcagcggt ggtgactggc tgatgttcgt
    12841 caccataacg gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt ggcggcagga
    12901 ccagcgactg catggcatca cgcttctcct ctatccggtc aaacacgccc accacctcgt
    12961 aagccccttc aataactttc tccaccacat ttccccggtg tggaacacgc aattctccca
    13021 gagactgacc gcagaccagt ccattggtac aaatggcacg aaaatatccg ggtaacatct
    13081 ggtagctgga tgaaccgtca tgagagttga gcagaataat ttcagggaca tgctgaccgg
    13141 ttatctgtcc ggcccgccgc agacgcagca tgtgttttgt gtattcccgg cgacccgggt
    13201 cacgcacacg tgtctggcag gcgaagaatg gctgaaagcc ttcccgctgc aggctttcca
    13261 gtacggtgat ggtgggagtg tacgcatagc gtttactgcg ggaagtatgc cggtcttcac
    13321 cgaaaatact gggaacatag tgcatcagtt cttcgtgtgt cagcggacgg tcacggcgta
    13381 tctggtttgc agcataacca aaacgactgg ctagtcgcat aatttgctcc ttatcggtgg
    13441 ttaagattta ctggtgtaat aaatgaaaaa gccacgtctc ccggagaaga cgcggcctga
    13501 tggacgaaat taatgaggtt tattgtctga gaagaccgtc actggcgaac cttccggcgt
    13561 cagtctctgc gttgcggata acccgacgga aatgagttcc ctggtcatcg tcgataaaca
    13621 cattgcggta agctgttgtg atgacaacag cctgctcgcg tgagaaaggg acttcgggca
    13681 ataaacgtga catacaacca gacctgtatt tggttccctg aaaatataag tccctgtcat
    13741 catggcagag ccgtatttac ttttgatatc aataaattaa taacgcgaag atgaaccgca
    13801 taacttacgc ctttaatttg ttttgtttac gtagtccctg accaggtacg cgaattcgtg
    13861 aacgccagtt tcaccaaacc aaatcaggta accagggtcg atgttgtaat agtaaccaac
    13921 gactttatgc gtttttgggt tatagagtgt tacgatatag tcccagccgc aggcgtgggc
    13981 tttacaaccg ccaccttcta cccaggtcgt tccgttaatg tttacaggat acgcaccacc
    14041 atcaggacca tccatatagc cgtcttcccc aaacattccg tttaacccaa cagggtttaa
    14101 tgctttgtcg atagcagctc tgtaggtagc gtcaccttcg tataactcgt acgttgttgg
    14161 acatgcacca ggctggtcag cccgggcttt tgagtcgcat ttaagtgtct gatttacagt
    14221 ggcggcgttt gtgtcaggac tt
//
LOCUS       DAJTXY010000102        13943 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTXY010000102 DAJTXY010000000
VERSION     DAJTXY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.102"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..380
                     /gene="tnpA"
                     /locus_tag="OO566_003099"
     CDS             <1..380
                     /gene="tnpA"
                     /locus_tag="OO566_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6606301.1"
                     /translation="TEKLRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSL
                     VNSLKGISNRLLRRDRPDIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPG
                     QVENRALYPRPEGRGFTAHRIKD"
     gene            complement(419..766)
                     /gene="grxD"
                     /locus_tag="OO566_003100"
     CDS             complement(419..766)
                     /gene="grxD"
                     /locus_tag="OO566_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6606302.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1101..1928
                     /gene="mepH"
                     /locus_tag="OO566_003101"
     CDS             1101..1928
                     /gene="mepH"
                     /locus_tag="OO566_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6606303.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2056..2637
                     /gene="sodB"
                     /locus_tag="OO566_003102"
     CDS             2056..2637
                     /gene="sodB"
                     /locus_tag="OO566_003102"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6606304.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2783..3952)
                     /locus_tag="OO566_003103"
     CDS             complement(2783..3952)
                     /locus_tag="OO566_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6606305.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4118..4207)
                     /gene="ynhF"
                     /locus_tag="OO566_003104"
     CDS             complement(4118..4207)
                     /gene="ynhF"
                     /locus_tag="OO566_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6606306.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4506..5531
                     /gene="purR"
                     /locus_tag="OO566_003105"
     CDS             4506..5531
                     /gene="purR"
                     /locus_tag="OO566_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6606307.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5528..6460)
                     /locus_tag="OO566_003106"
     CDS             complement(5528..6460)
                     /locus_tag="OO566_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6606308.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6573..7784
                     /locus_tag="OO566_003107"
     CDS             6573..7784
                     /locus_tag="OO566_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6606309.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8075..9223
                     /gene="cfa"
                     /locus_tag="OO566_003108"
     CDS             8075..9223
                     /gene="cfa"
                     /locus_tag="OO566_003108"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6606310.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9263..9904)
                     /gene="ribE"
                     /locus_tag="OO566_003109"
     CDS             complement(9263..9904)
                     /gene="ribE"
                     /locus_tag="OO566_003109"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6606311.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10119..11492
                     /gene="mdtK"
                     /locus_tag="OO566_003110"
     CDS             10119..11492
                     /gene="mdtK"
                     /locus_tag="OO566_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6606312.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11533..12789)
                     /locus_tag="OO566_003111"
     CDS             complement(11533..12789)
                     /locus_tag="OO566_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6606313.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13097..13173
                     /locus_tag="OO566_003112"
     tRNA            13097..13173
                     /locus_tag="OO566_003112"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13131..13133,aa:Val,seq:gac)
     gene            13178..13254
                     /locus_tag="OO566_003113"
     tRNA            13178..13254
                     /locus_tag="OO566_003113"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13212..13214,aa:Val,seq:gac)
     gene            13362..13667
                     /locus_tag="OO566_003114"
     CDS             13362..13667
                     /locus_tag="OO566_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6606314.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13793..>13943
                     /locus_tag="OO566_003115"
     CDS             13793..>13943
                     /locus_tag="OO566_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR6606315.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENS"
BASE COUNT         3410 a         3607 c         3312 g         3614 t
ORIGIN      
        1 cgacagaaaa actacgcact tacttttcaa atgtatgtgc tgattttgca gctgaactgg
       61 ttgaaatgga tggcgaacca gatcacgtcc atttgttaat taactatcct cccaaactgg
      121 cgatatccag tctggtaaac agcctcaaag gcatatcgaa taggttactg cgacgagatc
      181 gaccagatat tgcagtcagg tattactaca aaggcgtttt gtggagtcct ggctattttg
      241 ccagtagctg cgaaggtgcg ccaatatccg tcatccgcca atacattgaa caacagcaaa
      301 cacctggtca ggtggaaaac cgcgccttat atccccgccc tgaaggacga ggttttacgg
      361 cgcaccggat aaaggactga cggatgcccg ttcgcatccg tcagtattgc aggacggatt
      421 attccgcgtc cggctcttca gacttgtatt tagcggcagt ttctttgatc agctgctgca
      481 gttcgccacg ctgatacatt tcgatcacga tatcacaacc gccgaccagc tcgccgtcaa
      541 cccacagttg cgggaaggtc ggccagttag catatttcgg cagttccgca cgaatgtccg
      601 gattctgcag aatatcaaca taggcaaaac gttcgccaca tgcggcaagc gcctggactg
      661 cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc tttcatgtac agcaggatcg
      721 ggttttcagc aatctggcgt tggatttttt cgatagtggt gctcattgtc ttgcttcctc
      781 aaacttcttt acggcagtaa tctgacattg tagcgggtca gtgcggcatc ggaaaataac
      841 attttcatca cgcttttgct attttatccc tttgctctat ttgttgcatt tcaaatattc
      901 gtttcttctc tgatgcgcgg caaactggcg tatttataac catttttagc tttcactgct
      961 gcattttttt gcgctcgcca acgaaacgta ttttttaaca ataaaagcta ttaactttct
     1021 cttcttctat gcattagaat catcaagttt tgtaaatcag acgcaggcat gatagacctg
     1081 ccttaacaga gggacgctca gtggcgcgga taaaccgtat ttcgatcacg ctctgtgctt
     1141 tactttttac caccctgcct ttaacgccta tggcccatgc ttcaaagcaa gccagggaga
     1201 gttctgctac cgctcatatc accaaaaaag cagataaaaa gaaaagcacg gcaaccacca
     1261 aaaagaccca gaaagccagc cagaaaacag cgaaaaaagc cgccagtaaa agtacgacca
     1321 aaagcaaaac cgcttcttcc gttaaaaaat cttccattac cgcttctaaa aacgccaaaa
     1381 ctcgcagcaa acacgccgtc aataaaacgg cctcagccag ctttaccgaa aagtgtacca
     1441 aacgtaaggg ctataaatcg cattgtgtga aagtcaaaaa tgccgcgtca ggaacccttg
     1501 ccgacgcgca caaagcgaag gtgcaaaaag ccacgaaagt ggcaatgaat aaactgatgc
     1561 agcaaattgg taagccatat cgttggggcg gcagctcacc gcgtaccggt tttgattgca
     1621 gcggcctggt ttattacgct tataaagatt tggtgaaaat tcgtattccg cgtacggcga
     1681 atgaaatgta tcacctgcgt gatgcaggac ctatcgaacg tagtgagctg aaaaacggcg
     1741 acctggtctt tttccgtact cagggacgcg gtacagccga tcatgtcggc gtgtatgtcg
     1801 gcaacggcaa atttattcag tcaccgcgca caggtcagga aattcaaatc acttctctca
     1861 gtgaagacta ctggcagcgc cactatgttg gcgctcgtcg ggtaatgacc ccaaaaacac
     1921 ttcgctaaaa ctttaccctg ttgttacggc aacagggtaa gttcatcttt tgtctcacct
     1981 tttaatttgc taccctatcc atacgcacaa taaggctatt gtacgtatgc aaattaataa
     2041 taaaggagag tagcaatgtc attcgaatta cctgcactac catatgctaa agatgctctg
     2101 gcaccgcaca tttctgcgga aactatcgag tatcactacg gcaagcatca tcagacttat
     2161 gtcactaacc tgaacaacct gattaaaggt accgcgtttg aaggtaaatc actggaagag
     2221 attattcgca gctctgaagg tggcgtattc aacaacgcag ctcaggtctg gaaccatact
     2281 ttctactgga actgcctggc accgaacgcc ggtggcgaac cgactggaaa agtcgctgaa
     2341 gctatcgccg catcttttgg cagctttgcc gatttcaaag cgcagtttac tgatgcagcg
     2401 atcaaaaact ttggttctgg ctggacctgg ctggtgaaaa acagcgatgg caaactggct
     2461 atcgtttcaa cctctaacgc gggtactccg ctgaccaccg atgcgactcc gctgctgacc
     2521 gttgatgtct gggaacacgc ttattacatc gactatcgca atgcacgtcc tggctatctg
     2581 gagcacttct gggcgctggt gaactgggaa ttcgtagcga aaaatctcgc tgcataataa
     2641 ctgatggcaa atgcagcatt gcctgatgcg cttcgcttac catgccaacg cggatgaggc
     2701 gttaacagcg cattcggcaa gataaatcgc acgttgtcag caactgtaac gcagaaggtt
     2761 atccttctgc gtttttgttt aattagctgt tagcaacgca aactgtttca ggttgttttc
     2821 tggctgacat aaacaccagc aataatgcca gtcccgcgac aatcgccccc gtcaccggca
     2881 caaagctgta tcccagccca gcggaaatta ccgcaccacc agcagctgct cccagcgcat
     2941 ttccaagatt aaaggcacca atattgactg atgaagacag acctggcgct tcactggcga
     3001 catgcatcac gcgcatctgt aacggaggta caaccgcaaa ggttgctgcg ccccacacca
     3061 ccatgctaat agctgcgccg aactcattgc gggccaggaa cgggattgcc agcataatca
     3121 ccatcagcag caacaaaaag cctttcaacg tgccgttaac tgaacgatct gccagtttgc
     3181 cgccgagata gttaccgata gagaatccga caccaatcag caccagcatt gccgtgacga
     3241 acaccggtgt tgcgtgggta atactttgca gtaccggaga gatataggtg tagagggtaa
     3301 acattgcacc agctcccagt accgtcgtca gcaatgcaga cagcacctgc ggacgcatta
     3361 ataccgccag ctcttttttc acttcaggtc gtgcccctgt accaccttta ggtaatgaga
     3421 agaacagact taccattgaa atcactccca gccccgccgt tgccagaaat gacatccgcc
     3481 agccgatggt ttcacccaac caggtcgccg ccggcacgcc accgatattt gccagggtta
     3541 accccataaa catagtggca actgcgctgg cctgtttatg ttttggcacc acgcttgcgg
     3601 ccacgactga acccaaacca aaaaatgctc cgtgattcag gctggtcaaa atacgtgaaa
     3661 gcatcagggt catataatcc ggcgcgatgg cggaaagaac gttgcctagc gtgaaaattg
     3721 ccatcaggaa aatcaacgca ctgcggcggg cacgatgaga aagtagaagc gtcatcagcg
     3781 gcgcgcccac cattacgcca actgcatagg cactgattaa cattccggca gcgggaatcg
     3841 agacatccac accgcgcgca atgacgggca acaagcccat tggcgagaac tccgttgtcc
     3901 cgataccaaa cgcgccaatc gccagcgcca gcaacggata gttaattttc atgccttatc
     3961 tccacctctt cgcgtcatta cgcgatattc attaaagtgg cgaaagcatg acagcaatca
     4021 caaaaaaatg aaaataacaa aaagagaaaa cacttttgcc attttgctaa caaacaggaa
     4081 ggagatgcga gggagaacgc gctccctcga gaggaaatca gtgcagcgcg gcagtcaaac
     4141 ccacggctac gatcaaaccg aggacgataa tcgttgttac cagtgaaaat ttaaggtcgg
     4201 tgctcatcaa gttttctcct tttttattac cacacaaaaa gtgatattac gcatttttac
     4261 acactgttat gaaaaaatct cccgtcattt ataatgataa gtgtttttac cacttcccct
     4321 tttcgtcaag atcggccaaa attccacgct tacactattt gcgtactggc cattgacccc
     4381 ttcctgacgc tccgtgtcgt ttttccggcg taccgcaaca cttttgttgt gcgtaaggtg
     4441 tgtaaaggca aacgtttacc ttgcgatttt gcaggagctg aagttagggt ctggagtgaa
     4501 atggaatggc aacaataaaa gatgtagcga aacgagcaaa cgtttccact acaactgtgt
     4561 cacacgtgat caacaaaaca cgtttcgtcg ctgaagaaac gcgcaacgcc gtgtgggcag
     4621 cgattaaaga attacactac tcccctagcg cggtggcgcg tagcctgaag gttaaccaca
     4681 ccaagtctat cggtttgctg gcgaccagca gcgaagcggc ctattttgcc gagatcattg
     4741 aagcagttga aaaaaattgc ttccagaaag gttacaccct gattctgggt aatgcgtgga
     4801 acaatcttga gaaacagcgg gcttatctgt cgatgatggc gcaaaaacgc gtcgatggtc
     4861 tgctggtgat gtgttctgag tacccagagc cgttgctggc gatgctggaa gagtatcgcc
     4921 atatcccaat ggtggtgatg gactggggtg aagcaaaagc tgacttcacc gatgcggtca
     4981 ttgataacgc gttcgaaggc ggctacatgg ccgggcgtta tctgattgaa cgcggtcacc
     5041 gcgaaatcgg cgtcatcccc ggcccgctgg aacgtaacac cggcgcaggc cgccttgccg
     5101 gttttatgaa ggcgatggaa gaagcgatga tcaaggtgcc ggaaagctgg attgtgcagg
     5161 gtgactttga acctgaatcc ggttatcgcg ccatgcagca aatcctgtcg cagccgcatc
     5221 gccctactgc cgtcttctgt ggtggcgata tcatggcaat gggcgcactt tgtgctgctg
     5281 atgaaatggg cctgcgcgtc ccgcaggatg tttcgctgat cggttatgat aacgtgcgca
     5341 acgcgcgcta ttttacgccg gcgctgacca cgatccatca gccaaaagat tcgctgggtg
     5401 aaacagcgtt caacatgctg ttggatcgta tcgtcaacaa acgtgaagaa ccgcagtcca
     5461 ttgaagtgca tccgcgtttg attgaacgcc gctccgtggc tgacggcccg ttccgcgact
     5521 atcgtcgtta atcacccgtt gcgggagtct cttccggctc ccgcagccac tccttattca
     5581 gcgtctcact atcgccgaga tactcaagca gccaggttaa cgcaggcgac atatcatttt
     5641 gctgccatgt cagacaacat gccgaatccg ggaaggggtt ttccagttca agtgctaccc
     5701 acttcccctc attaagccac ggtttggcga aatgtgttgg caccatccct atgcataatc
     5761 ctgccgagat acaggttgcc gatgattccc agtcaggtac gacaagtctt ttttggttat
     5821 ccagcaacca ggtaatacgt ttaggtagcg ttcgcgaggt gtcttcgcgc accaacgacg
     5881 gccagttgcg caacgtatca tcgctgaacg ggccatccat caacgccagc gggtggtgac
     5941 tggcaacaac gcaactccag cttagcatcc ccatatcccg gaaggcataa cgaccgccta
     6001 ccggaatcgc gcgtgttgcg ccaattgcca gttccacgcg cccgtcggaa agcgcatccc
     6061 agacaccgtt gaacacttcc tgaaagacaa gaagttcaac atcatcaaaa tggcgataaa
     6121 aatcaacgat catctgccgt gtacgttctg gcctgacaat attatccact gcgatagcta
     6181 actgaccgcg ccagccgttc gctatctgct gacattgctg gcgggtgatc tgcatttttt
     6241 tgacaacaga gcgcccttct ttgagaaacc acgctccagc agcggtcagc tccacatcac
     6301 ggtgccgtcg ttcaaagagc ggtaccgcca gccactcttc cagctgacgc acggtatagc
     6361 tgaccgcaga aggaacgcga tgcagctcct gtgccgcagc gctaaaacta ccattacgcg
     6421 ctaccgcatc aacaacttcg agtgaatatt ctgaccacat agtcagcctg caaaattttt
     6481 gaaaccagtc atcaaatatt accgtttcac aacactaatt tcactcccta cactttgcgg
     6541 cggtgtttaa ttgagagatt tagagaatat acatgcaacc tgggaaaaga tttttagtct
     6601 ggctggcggg tttgagcgta ctcggttttc tggcaaccga tatgtatctg cctgctttcg
     6661 ccgccataca ggccgacctg caaacgcctg cgtctgctgt cagtgccagc cttagtctgt
     6721 tccttgccgg ttttgccgca gcccagcttc tgtgggggcc actctccgac cgttatggtc
     6781 gtaaaccggt gttattaatc ggcctgacaa tttttgcgtt aggtagtctg gggatgctgt
     6841 gggtagaaaa cgccgccacg ctgctggtat tgcgttttgt acaggctgtg ggggtctgcg
     6901 ccgcggcggt tatctggcaa gcattagtga cagattatta tccttcacag aaagtcaacc
     6961 gtatttttgc gaccatcatg ccgctggtgg gtctgtctcc ggcactggct cccctgttag
     7021 gaagctggct gctggtccat ttttcctggc aggcgatttt cgccaccctg tttgccatta
     7081 ccgtggtgct gattctgcct attttctggc tcaaacccac gacgaaggcc cgtaacaata
     7141 gtcaggatgg tctgaccttt accgacctgc tacgttctaa aacctatcgc ggcaacgtgc
     7201 tgatatacgc agcctgttca gccagttttt ttgcatggct gaccggttca ccgttcatcc
     7261 ttagtgaaat gggctacagc ccggcagtta ttggtttaag ttatgtcccg caaactatcg
     7321 cgtttctgat tggtggttat ggctgtcgca ccgcactgca gaaatggcaa ggcaagcagt
     7381 tattaccgtg gttgctggtg ctgtttgctg tcagcgtcat tgcgacctgg gctgcgggct
     7441 tcattagcca tgtgtcgctg gtcgaaatcc tgatcccatt ctgtgtgatg gcgattgcca
     7501 atggcgcgat ctaccctatt gttgtcgccc aggcgctgcg tcccttccca catgcaaccg
     7561 gtcgcgccgc agcgttgcag aacactctgc aactgggtct gtgcttcctc gcaagtctgg
     7621 tagtttcctg gcttattagt atcagcacgc cattgctcac caccaccagc gtgatgttat
     7681 caacagtagt gctggtcgcg ctgggttaca tgatgcaacg ttgtgaagaa gttgactgcc
     7741 cgaatcatgg caatgccgaa gtcgctcata gcgaatcaca ctgatctata tcgatatact
     7801 tatacttagg ctgctaacaa aattttgttg tatgattgaa attagcggcc tatactaatt
     7861 tcgagttgtt aaagctacga taaatattat gtttttacgg ggacaggatc gttcccgact
     7921 cactatggat ggtcatttcg gcaagggttc ctcctttccc tctgttctac gtcggattat
     7981 agactcgcgg ttttttctgc gagatttctc acaaagccca aaaagcgtct acgctgtttt
     8041 aaggttctga tcaccgacca gtgatggaga aactatgagt tcatcgtgta tagaagaagt
     8101 cagtgtaccg gatgacaact ggtaccgtat cgccaacgaa ttacttagcc gtgccggtat
     8161 agccattaac ggttctgccc cggcggatat tcgtgtgaaa aaccccgatt tttttaaacg
     8221 cgttctgcag gaaggctctt tggggttagg cgaaagttat atggatggct ggtgggaatg
     8281 tgaccgactg gatatgtttt ttagcaaagt cttacgcgca ggtctcgaga accaactccc
     8341 ccatcatttc aaagacacgc tgcgtattgc cggcgctcgt ctcttcaatc tgcagagtaa
     8401 aaaacgtgcc tggatagtcg gcaaggagca ttacgatttg ggtaatgact tgttcagccg
     8461 catgcttgat cccttcatgc aatattcctg cgcttactgg aaagatgccg ataatctgga
     8521 atctgcccag caggcgaagc tcaaaatgat ttgtgaaaaa ttgcagttaa aaccagggat
     8581 gcgcgtactg gatattggct gcggctgggg cggactggcg cactacatgg catctaacta
     8641 tgacgtaagc gtggtcggcg tcaccatttc tgccgaacag caaaaaatgg ctcaggaacg
     8701 ctgtaaaggc ctggatgtca ccattttgct gcaagattat cgtgacctga acgaccagtt
     8761 tgatcgtatt gtttctgtgg ggatgttcga gcacgtcgga ccgaaaaatt acgataccta
     8821 ttttgcggtg gtggatcgta atttgaaacc ggaaggtata ttcctgctcc atactatcgg
     8881 ttcgaaaaaa accgatctga atgttgatcc ctggattaat aaatatattt ttccgaacgg
     8941 ttgcctgccc tctgtacgcc agattgctca atccagcgaa ccccactttg tgatggaaga
     9001 ctggcataac ttcggtgctg attacgatac tacgttgatg gcgtggtatg aacgattcct
     9061 cgccgcatgg ccagaaattg cggataacta cagtgaacgc tttaaacgga tgtttaccta
     9121 ttatctgaat gcctgcgcag gtgctttccg cgcccgtgat attcagctct ggcaggtggt
     9181 gttctcacgc ggtgttgaaa acggccttcg agtcgctcgc taaaggctat tctatcgccc
     9241 cctcttcggg ggcgagttca gatcaggctt ctgtacctgg ttgattcatg gcattttctc
     9301 gtgccgccag cacacgttct accgtatcta ccactgcctg agtttgtgga tcgatttcaa
     9361 tgttgacgcg tgcgccaagt tttttcttcc caagagtcgt gcgttccagt gtttccggaa
     9421 ttaaatggac gcaaaaacgc gttggcgtga cttcgccgac ggtcaggcta ataccgtcga
     9481 tgccaataaa tcctttgtac aggatatatt tcatcaactg actatcctgg actttaaacc
     9541 agatctggcg attattttcc gaggttaata ttttcgccac ttcagcggtg gtcataatat
     9601 gacctgacat taagtgtccg ccaatttcat cactgaattt cgccgcacgc tcaacgttta
     9661 cccaatcccc cacttttaaa tcgccaagat tggtaatgcg taacgtttct ttcatcaggt
     9721 caaaactgac atggttgccg ttaatttccg tcacggtcag gcagcaaccg ttatgcgcca
     9781 cggaagcacc ggtttccagg ccgtccagca tgtggtcggg taactccacc acatgcgtac
     9841 gaaaatttgg tttctcgtca atcgacacca gttttgcggt gccctgtaca atccccgtaa
     9901 acatacttac aactcctgaa atcagttaag acattctgtt cagcacaata gcaggtggaa
     9961 aacgccctta ccagtgaagg ggtaagaatg gctatttttt cactggagaa ttaataaatc
    10021 ctcgctacaa tagactgaat ttcccctgct tcttcttttt gctgcccatt caggcggctt
    10081 tttagtctct catataacta caaataaaag gtgttcacgt gcagaagtat atcagtgaag
    10141 cgcgtctgtt attagcatta gcaatcccgg tgattctcgc gcaaatcgcc caaactgcga
    10201 tgggttttgt cgataccgtg atggcgggcg gctatagtgc caccgacatg gcggcggtcg
    10261 ctatcggtac ttctatctgg cttccggcga tcctctttgg tcacggactg ctgctggcat
    10321 taacgccggt tatcgcgcaa ttaaatggtt ccggtcgacg tgagcgcatt gcgcatcagg
    10381 tgcgacaagg tttctggctg gcaggttttg tttccgttct cattatgctg gtgctgtgga
    10441 atgcaggtta cattatccgc tccatggaaa acatcgatcc ggctctggcg gacaaagccg
    10501 tgggttatct gcgtgcgttg ttgtggggcg cgccgggata tctgttcttc caggttgccc
    10561 gtaaccaatg tgaaggtctg gcaaaaacca agccgggtat ggtaatgggc tttatcggcc
    10621 tgctggtgaa catcccggtg aactatatct ttatttatgg tcatttcggt atgcctgagc
    10681 tcggtggcgt tggttgtggc gtggctactg cggcggtgta ttgggtcatg ttccttgcca
    10741 tggtttctta cattaaacgc gcccgctcca tgcgcgatat tcgtaacgaa aaaggcaccg
    10801 ctaagcccga tcctgcggtt atgaaacgac tgattcaact cggtttgccg attgcgctgg
    10861 cactgttctt tgaagtgaca ctgtttgccg tcgtggctct gttagtgtct ccgctcggta
    10921 ttgttgatgt cgcaggacac cagattgccc tgaactttag ttcactgatg tttgtgcttc
    10981 caatgtcgct ggcggcagcg gtaactatcc gcgtaggtta tcgtctgggt cagggctcaa
    11041 cgctggatgc gcaaaccgct gcgcggaccg ggcttatggt gggtgtctgt atggcaaccc
    11101 tgacggccat tttcacggtt tcactgcggg agcaaatcgc cctgttgtac aacgacaatc
    11161 ccgaggttgt aacgctggct gcgcatttga tgttgctggc ggcggtatat cagatttctg
    11221 actcaatcca ggtgattggc agtgggattt tgcgtggtta taaagatacg cgttccattt
    11281 tctatatcac ctttacagct tactgggtgc tgggcttgcc aagcggctat attctggcac
    11341 tgaccgatct ggtcgttgaa cctatggggc cagcaggctt ctggataggc tttattatcg
    11401 gcctgacatc ggcagccatt atgatgatgc tgcgtatgcg gttcctgcaa cgtatgccgt
    11461 cagccatcat tctgcaacga gcgtcccgct aataaagaca aggcgcaacc ttcacgggtt
    11521 gcgcctgtgt ttttacgcag gctggagcgt tgcgccaatc ccgtcttcgt ctggctgtaa
    11581 tttcagagcg ttacacagag tttgccgact ggcatctgtt atctcaacgc gtatatcccc
    11641 gttaacttcc cctttcacat tgatgaagtc aggtgaaatt tctttcgcac tggtttgcag
    11701 gcaaatcgct ccctctccgc gcaggcgttt cactttcagt tgcacatgcg ttgccgcacc
    11761 agtgatcagc cacagttttc cgccattcag attgatgtta ccaacaatga aagtgacgct
    11821 gtcacctttt accgtactgc cgtcgagaat caacacaccg ccctgctgta cagtaatcgt
    11881 tgattgttgc agctctgcgc cataaccaat aatcattgaa gcgccgtcca gtaatgcaac
    11941 actcccggtc agttcaaccg gtgcgcgatc ttcctctacg acaaaatttt cgtccgcgct
    12001 ggtgggtgac aacaggatca ctgttccacc ttgtattacc gcattttcca gcgtaccggc
    12061 atgcacttca gcccgatccc ccacattcac cgacaggttt tgcgtcttac cggaactgta
    12121 gagctgaagg ccatccgtcc ccagacgata aatggatcca ttctcaacaa tagtgtcaga
    12181 gtcttcacca ccgagattat tcatggtgcc accagcaagc aacgtatcac tggctttccc
    12241 ggccagcata aaaacgccgc cctgattaat ctcacaattg ctggcttctc cacttggata
    12301 caccaacaat tcaccacctt catctaccac gaccgctgag gttgtcccat taaccaacag
    12361 gccgccctct tgatcgagaa tgattttttc cgcgcgatgt ccttccagta cacgcaggtt
    12421 accgccattt tccagcaaca aaccgtaggc ataaccctga tcaacgctga attcaccttc
    12481 gggatggcgg ccattaaccg tagcgagcgt agagagacta attgcgcctc cctcggactg
    12541 aacaatatct gtggcaatgg caccgtcgct gaccgatagc gtgccattgt taccgtactg
    12601 tgtgcatttg gcgatcccac cggttgccac tttcaaccag ccagcccccg tgaccacggt
    12661 attttcggct cttccgccag cttcaacaat caactcgcct tcagtaagtt gcgtcgcgcc
    12721 aatgacctcg ccggtcttaa caatttgcac attcccgtcg ctcatcaagt ttttcgcatc
    12781 agatcccatg atttattcct ttgctgcatc tgtgtgcctt tattgctacc taagtgtaaa
    12841 ggctacggag gatttatcca cgacagattt gagatggtgg caaacaactc tgtttaaact
    12901 ctgatacacg aattattggg ttgtatcaga tgtaaatgcg atcctgaata aaaatcgccc
    12961 ttgcaaatca acaaaatata gccaatttgc ttaaaagctc tgcaaacggt cgaaatagcg
    13021 cagaaaatta cgttttgcct cttgccacct ttccactctg ccgctaatat tcgtccccgt
    13081 tgtcacctac aacgttgcgt tcatagctca gttggttaga gcaccacctt gacatggtgg
    13141 gggtcgttgg ttcgagtcca attgaacgca ccatcctgcg tccgtagctc agttggttag
    13201 agcaccacct tgacatggtg ggggtcggtg gttcgagtcc actcggacgc accagatttt
    13261 cttaatctgg tcttttcctt tttccctctg tttcttctct gtatccaata cgttaaaaga
    13321 tttacactgt cttcgtatgc gttatcagaa ggagaatcgc tatggcaact ttgttacaat
    13381 ttcattttgc ttttaatggc ccatttggcg acgcaatggc tgagcagctt aaaccacttg
    13441 ctgagtcgat taatcaggaa cctggttttc tgtggaaggt atggacagaa agtgaaaaga
    13501 accacgaagc cggtgggatc taccttttca ctgatgaaaa aagcgccctt gcctatctgg
    13561 aaaaacatac tgcccgactg aaaaatctcg gcgttgagga agttgtcgcc aaagttttcg
    13621 atgtcaatga gccacttagt caaatcaatc aggcaaaact cgcctgacag aatttaatca
    13681 agggcggtta gcgccctttt catccctgtc tgaaatttct caaattctaa aaatctcaac
    13741 caaacttatc tgataacact aaattcgaaa gaatgcgtac aggtaagtaa caatgaaaaa
    13801 aattgctatt gtgggtgccg ggcctacggg gatctacacc ttattctcgc ttctacagca
    13861 acaaactcca ctttctattt ctatcttcga gcaggctgac gaggccggtg tcgggatgcc
    13921 atacagtgat gaggaaaact caa
//
LOCUS       DAJTXY010000103        13906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTXY010000103 DAJTXY010000000
VERSION     DAJTXY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.103"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            243..1496
                     /gene="kch"
                     /locus_tag="OO566_003116"
     CDS             243..1496
                     /gene="kch"
                     /locus_tag="OO566_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6606316.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1551..1724)
                     /locus_tag="OO566_003117"
     CDS             complement(1551..1724)
                     /locus_tag="OO566_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6606317.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1867..3327
                     /gene="cls"
                     /locus_tag="OO566_003118"
     CDS             1867..3327
                     /gene="cls"
                     /locus_tag="OO566_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6606318.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3362..3691
                     /locus_tag="OO566_003119"
     CDS             3362..3691
                     /locus_tag="OO566_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6606319.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3744..4748)
                     /gene="oppF"
                     /locus_tag="OO566_003120"
     CDS             complement(3744..4748)
                     /gene="oppF"
                     /locus_tag="OO566_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6606320.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4745..5758)
                     /gene="oppD"
                     /locus_tag="OO566_003121"
     CDS             complement(4745..5758)
                     /gene="oppD"
                     /locus_tag="OO566_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6606321.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGAATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5770..6678)
                     /gene="oppC"
                     /locus_tag="OO566_003122"
     CDS             complement(5770..6678)
                     /gene="oppC"
                     /locus_tag="OO566_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6606322.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6693..7613)
                     /gene="oppB"
                     /locus_tag="OO566_003123"
     CDS             complement(6693..7613)
                     /gene="oppB"
                     /locus_tag="OO566_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6606323.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7699..9330)
                     /gene="oppA"
                     /locus_tag="OO566_003124"
     CDS             complement(7699..9330)
                     /gene="oppA"
                     /locus_tag="OO566_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6606324.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10068..10715)
                     /gene="ychE"
                     /locus_tag="OO566_003125"
     CDS             complement(10068..10715)
                     /gene="ychE"
                     /locus_tag="OO566_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6606325.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11192..13867
                     /gene="adhE"
                     /locus_tag="OO566_003126"
                     /gene_synonym="adhC"
     CDS             11192..13867
                     /gene="adhE"
                     /locus_tag="OO566_003126"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6606326.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3647 a         3445 c         3238 g         3576 t
ORIGIN      
        1 ccttatagcc ggtcagtgca ttcactacca caagcagata ataactttat ctgtatttct
       61 ttatatgatg tgagcatgtt caggaaaaaa gccctttcct gacgcagagt tgctcactca
      121 aaacctttga ttactgcgga taaagtcatc tactattgtt taacacattc acgcgctgca
      181 tgtatgttca gaaaatgtgc acgctaagat aattaaacaa aattgtggtt acagaggtat
      241 cagtgagtca ctgggctaca ttcaaacaaa cagcaacaaa tttatgggtg accctacggc
      301 acgacattct cgcgctggcc gtctttttaa atggattgct tatttttaaa acaatctatg
      361 gtatgtcggt caatttgctt gatattttcc atatcaaagc attttcagag ttggatctct
      421 ccttgctggc aaacgcccca ctatttatgc tcggcgtctt tcttgtcctg aactccattg
      481 gcttactgtt ccgggcaaag ctcgcatggg caatcagtat cattttgttg ttgatagcgc
      541 taatttacac cctgcatttt tatccctggc tgaaatttag tattggattt tgcattttta
      601 cgctggtgtt tttgctgata ctgcgcaaag acttctccca cagtagcgcc gcagccggga
      661 caatttttgc atttattagt ttcacgacat tactgtttta ctcaacctac ggtgcacttt
      721 atttaagcga aggttttaat ccgcgaatag aaagtttgat gaccgcgttc tatttttcga
      781 tagaaaccat gtcaaccgtc ggctatggag atattgtccc tgtttctgaa tcagcacgat
      841 tgttcactat ttcggtcatt atttccggca ttaccgtttt tgccacatct atgacctcaa
      901 tttttggtcc gcttatccgc gggggattca acaaactttt aaaaggaaac aatcatacaa
      961 tgcatcgtaa agatcatttt attgtttgcg gacattcgat tctcgccatc aatacgatcc
     1021 tgcaactgaa tcaacgcgga caaaacgtaa cggttatcag caacttgcct gaagatgata
     1081 tcaagcaact tgagcaacgc ttaggcgata acgctgatgt tatccccggt gacagtaatg
     1141 acagttcagt attaaagaaa gcgggaatcg atcgttgccg ggccattctg gcgctgagtg
     1201 ataacgatgc agataacgcg tttgttgtac tctcggcaaa agatatgagc agtgatgtca
     1261 aaacagttct cgccgtcagt gatagcaaaa acctgaataa gattaagatg gtacatccgg
     1321 atatcattct ctcaccgcaa ctgtttggca gcgaaattct ggcgcgagtt ttaaatggtg
     1381 aagagattaa taatgatatg ctcgtttcaa tgttgttgaa ctcaggtcat ggtattttca
     1441 gcgataacga tgaacaagaa acgaaagctg acagtaaaga atcggcgcaa aaatagagtt
     1501 taacgacgga agagtaaact tattgggcac cgcaatggtg cccagtacat ttagatattg
     1561 tagatcgcaa acaataacga gttacgctgt ttgtttagaa tgcacttcct gatgctgtgg
     1621 atacgcatat ttcggcgcga ttgcccctca agccaacgcg atttacgtcg gctagcctga
     1681 cgctgcatgc gccagcgccc cacttccgtt ctactccgct tcatgttaac tactctatgc
     1741 aataacagaa tggtcattat acccccgccc ccgacgctga ccagtgcttt tcccgcgttt
     1801 ttatttgcca gatgaatcca tatgcgtaaa ctcataacaa tgcgctttca aaaggatttc
     1861 taatctatga caaccgttta tacgttggtg agttggttgg ccattctggg atactggttg
     1921 ctcattgcag gcgtaacttt acgcattcta atgaaacgac gcgcagttcc ctccgcgatg
     1981 gcctggctgt tgattattta cattctgccg ttagtcggaa ttattgccta tcttgccgtt
     2041 ggcgagctcc atttaggcaa acgccgcgct gagcgcgcca gagcgatgtg gccttccacc
     2101 gcaaaatggc ttaacgacct taaagcctgt aagcatatct tcgccgaaga aaatagcagt
     2161 gtcgctgcgc cattattcaa gctttgcgag cgtcgtcagg ggatcgctgg ggtcaaaggg
     2221 aatcagctac aactgatgac cgagtcagat gatgtgatgc aggcgttaat ccgcgacatc
     2281 cagctcgcgc gccataatat tgagatggtg ttttatatct ggcagcccgg cggcatggcg
     2341 gaccaggtgg ctgaatcatt aatggcggct gcacggcgtg gtattcattg ccgattgatg
     2401 ctcgactccg ccgggagtgt ggcttttttc cgcagcccgt ggcccgagct aatgcgtaat
     2461 gccggtattg aagtggtcga agccttaaag gtcaatctga tgcgtgtgtt tttacgccgt
     2521 atggacctgc gccaacatcg caagatgatc atgatcgata attacatcgc gtacaccggc
     2581 agcatgaata tggtcgatcc tcgctacttc aaacaagatg cgggcgtagg gcaatggatt
     2641 gatctgatgg cgcgtatgga aggccccatc gccaccgcga tggggattat ttattcctgc
     2701 gactgggaga ttgaaaccgg aaaacgtatt ctgccgccac caccagatgt caatattatg
     2761 ccgtttgaac aggccagtgg tcacaccatt cacacaattg cttctggccc cggctttccg
     2821 gaagatctca ttcaccaggc attattgact gcggcttatt cggcgcatga atatttgatc
     2881 atgaccacgc cctactttgt gccaagcgat gatttacttc atgcgatttg cacggcggcg
     2941 cagcgcgggg tggatgtcag tattatcctt ccgcgaaaaa atgactcgat gctggtcggc
     3001 tgggccagtc gcgcattctt tacggaactg cttgctgctg gggttaaaat ttatcagttt
     3061 gaaggcgggt tactgcatac caagagcgtg ctggtcgatg gcgaactaag tctggttggc
     3121 accgttaacc ttgatatgcg tagtctgtgg ctgaatttcg agattaccct ggcaatcgac
     3181 gataaaggtt ttggtgcaga ccttgccgcc gttcaggacg attatatttc gcgttcacgt
     3241 ctgctcgatg cccgtttatg gctaaaacgt ccattatggc aacgtgtcgc cgagcgactg
     3301 ttttacttct tcagcccgtt gctgtaaaac gtcgccaaca gaggttaaac aggtagtgac
     3361 tatggatatg gatctaaaca atcgcctgac tgaagatgaa acgcttaagc aggcctacga
     3421 tatttttctc gaactggctg ccgacaatct cgatccagct gatgttctgc tgttcaatct
     3481 tcagtttgaa gagcgcggcg gcgcggagtt attcgatccg gcggaagact ggcaggaaca
     3541 tgttgatttc gatttgaacc cggacttttt tgccgaagtg gtgattggtc tggcggacag
     3601 tgaagatggt gaaatcaacg atgttttcgc ccgcattctg ttatgccgcg aaaaagatca
     3661 caaactttgc catatcatct ggcgggaata aataatcgct tttagcgtga aaaagggctg
     3721 acaactgtca gcccttattg tttttaaagc ggatcgactt tcaggcaaga aacggcgtgt
     3781 ctgaaactcc cctccagaac aggacgtgtt ttggcgcact ccggaccggc aatcgggcaa
     3841 cgggtacgga aaacacaacc ggaaggcggg ttgatcggcg acggtaattc cccttccagt
     3901 aactggatgg ttttgttctt ctccagatcc ggatcaggta tggggactgc cgacatcaat
     3961 gccctggtgt aaggatgtag tggattgtgg tagacctcat cataggtccc cagttctact
     4021 gcatggccga gatacatcac caacacacga tcggaaatgt gttttaccac ggccaggtca
     4081 tgagcaataa aaattaatga caatcccatc tcacgttgca gctgctggag caggttgacc
     4141 acctgcgcct gaattgacac gtccagcgcc gaaaccggct catcgcagat aatcagcttc
     4201 ggttcaagaa taagagcacg agcaatcccg atacgctggc actgcccacc ggagaactca
     4261 tgcggatagc ggttaatcag gttaggcaat aacccgactt tcagcatcat cgccttcacg
     4321 cgctcgcgaa cttcctggcg tgacattttc ggatgataag tacgcagtgg ttcagcgatg
     4381 atctcgccga tggtcatacg cgggtttagc gatgccaacg gatcctggaa aatcatctga
     4441 atatcactgc gaacggcacg ccattcatcg ggcttcatgc ccagcaactc tttacctaac
     4501 caggcaacat gaccgtcggt cgccttgacc aaaccgatga tggcgcgagc aaaggtggac
     4561 ttaccgcatc ccgattcccc taccacacct aatgtttccc cttcatacag gcgaagagtg
     4621 acaccatcga cggctttgag cgttttcggc ggttgccaga accactgttt gccatctttg
     4681 atttcaaagt gcactttaag atcggcgatt tcaaggagga cttttcttcc ttcagttaca
     4741 gcattcataa cagttcctcc accggtttaa agcaagcacg cagacggcca ggcgtaaact
     4801 cttccagcgg cggtgcgcta ctacaaattt ccatcgcatg tggacaacgt ggctggaacg
     4861 ggcaaccttt cggtaatcgc agcaagtttg gcggattacc cgggatggtc aacattgttt
     4921 caccttccgc atcaagacgc ggcaccgcgt tgagcaaacc gatagaataa ggatgaacgg
     4981 gttgataaaa gacatcgcgc gcattgccat attccatcgt gcgcccggcg tacattacca
     5041 gcactttgtc acagatcccc gccaccacgc caagatcgtg ggtgatcata atgatggcag
     5101 tattaaattc ccgcttcagt tcattcaata gcgtcatgat ctgcgcctgt acggtgacgt
     5161 ccagcgctgt agtgggttca tccgcaatca gcaacttagg tcgacatagc aatgccatcg
     5221 caatcatgac tcgctgacgc atgccgccag aaaattcgtg cgggtacatt ttcatgcgtt
     5281 tacgtgcttc cggcattttt accgcatcga gcatccgcac cgactcttca aacgcctcag
     5341 ctttgctcat gttcttatgc agcatcagca cttccatcaa ctgctcaccg acgcgcatat
     5401 agggattcaa cgaagtcatt gggtcctgaa aaatcattga gatttgttca gcgcgcagtt
     5461 tattgagttc acgctctggc aaattgagga tttcacgccc attgaaggtt gccgctccgc
     5521 caatgcgtcc gttggcagcc aacaggccca tcaacgcaaa tgcagtttgc gatttacccg
     5581 aaccagactc acctacaatg cccagcgttt ctccggcacg tagggaaaaa tttaaatcat
     5641 tgaccgccgt gacgtcgccg tccggggtac taaaggtgac acgcaaatct ttcacgttca
     5701 gcagtgcgtc agcctgttgt tgtgcgagcg gcacagttgc agtttcaatt acgctcatgg
     5761 ctgcactcct taacgatctt tcgggtcgag ggcatcacgc aagccatcgc cgataaagtt
     5821 gaaacaaaac agtgtcacca caaggaatcc cgctgggaac aacagtaacc atggagagac
     5881 ttccatcgag ttcgcgccat cactcagcaa tgccccccag ctgcttaacg gctcttgcgt
     5941 acccaacccc aggaagctaa ggaaagattc aaagaggatc atgctgggca ccagtagcga
     6001 tgcgtagacc accaccacac cgagtacgtt cggcacaatg tggcgaataa caatgcccgg
     6061 cgtcgataca ccgccaactt gtgccgcctc aataaactct ttgcgcttca gactcagggt
     6121 ttgcccacgc acaatacgag ccatatccag ccaggaaacc atgccaatcg ccacgaaaat
     6181 cagcaggatg ttttggccga aaaaggtcac cagcaaaatg acgaagaaca tgaatgggaa
     6241 ggagttgagg atttccagca gacgcatcat taccgaatcc actttaccgc ccagataacc
     6301 ggaaagcgaa ccataaagtg tccccacgac cactgccacc agtgccgcag caacaccgac
     6361 catgagtgag atacgcccgc caatcgcaac gcgcacaagc aggtcgcgac cggatgagtc
     6421 agtaccaaag tagtgaccgg actccatatc cggggcgctg gacatcatcg cccagtcagt
     6481 atcgtcatag gcaaactggg aaagcatcgg tgccaggatt acaaataacg cgatcagcac
     6541 cagcactatc agactggcaa ccgccgcacg gttatgcata aaacgtcgac gtgcgtcctg
     6601 ccacaagctg cgcccttcga cctccagctt ttcactgaaa ttttccagcg tctcgctgtt
     6661 tttcttactt aacatcatcg cgagctccag tatcagtaac ggattttcgg gtcgataacc
     6721 gcatatagca catcgacaat ggcattaaac aaaatggtta acgcaccaac caggatggtc
     6781 aggcttaaca ctaaggaata gtcacggttc aatgcgccat taacgaacag ttgcccaatc
     6841 cccggcaaac cataaatggt ttcgatgacc atagaaccgg taataatgcc gacaaatgcg
     6901 gggcccatat aggagagcac gggtaacaga gcaggtttta atgcgtggcg taaaatgatc
     6961 cgccgcatag gtaacccttt cgcccgggca gtacgaataa agttggagtg taatacttca
     7021 atcatagagc cacgggtaat acgcgcaata ctggcgatat aggcgagtga caacgccacc
     7081 atcggcaata tcatgaattt aagcgcccca ccattccagc caccgcccgg cagccaatgc
     7141 aaaatgatcg caaatatcat gactaataat ggcgcaacca caaaactggg gataacaacc
     7201 ccggtcattg ccagccccat cacggtatag tcccatttgg tgttttgttt taatgcggca
     7261 ataacgccag cactaacacc cagtattacc gcaaggaaaa atgcggcggc tcccagtttg
     7321 gcagaaacgg gaaaactgga tgccaccagg tcattgaccg aataatcttt atatttaaac
     7381 gatggaccga aatcgccatg cgccagttgt ttcaggtagc tgaaatactg tgtcatgatc
     7441 ggatcattaa gatgatattt cgcttcgata ttggccatca cttctggcgg taaagtacgt
     7501 tcgccggtaa aagggcttcc cggcgcgagg cgcatcataa agaacgaaat agtaataaga
     7561 ataaatagcg tcggaatcgc ttccagacag cgacgtagaa taaattttaa cattgcccta
     7621 ccttctggcc tgtgccttct gtaatgcgat aaaaatcaga caccgtggag caggacactc
     7681 ctgccccacg tattgccatt agtgcttcac aatgtacata ttccgggtat aggtattatc
     7741 cagcggatct ttgccggtat agccaccaac ccacggtttc accagacgcg cattcacgta
     7801 gtaataaaca ggaacaatgg ccgaatcctt atccagctgt tgttctgctt tagtgtacag
     7861 agctgtgcgc tgcgcctcgt cagtcacttt cagcgtttcc gccataatgc tgtcaaaggc
     7921 cgggctctta taatgcgcgg tattcatcga gctgttcgac agcatggtgt tcaggaagga
     7981 agttggttcg ttgtagtctg cacaccagcc tgcacgggcc acatcaaaag tgccctggtg
     8041 acgggtgtcg aggaacgttt tccactcctg gttaaccagt ttgacgttta caccaatgtt
     8101 tttcttccac aatgaagagg cagcaatcgc cagcttttta tgcagatcgg aggtgttata
     8161 ccgcaggttg atggtcaacg gtttgtctgc ggtataaccc gcttcagcca gcagtttttt
     8221 cgcttcttcg ttacgttttt cctggctcca gccaaaccat tctggctgag tcaattttgc
     8281 gccatcagta tacggtggag tgtaaccata ggcgggcatg tcgccctgcg ctttcacttt
     8341 attaacaatg atatcgcggt ccatacctag tttcagtgcg gtacgcacac gcacatcgtt
     8401 gaatggcggt ttctggttgt taatttcgta atagtaagtg cacaggtatg gatcaacgtg
     8461 aacttcgtcc gggatctctt ttttcagctt ctggaacaat tcgatcggca tgttgttata
     8521 cgtcatgtcg atttcaccac tacggtagcg gttgacatcg gtaacttcag aagcaatagg
     8581 caaataggtt acctggttaa taacggtttt cgcgttgttc cagtaggtcg ggctgcgttc
     8641 aagaacgatt cgttcgttta cgacccaatc ttttaaggta taggcaccgt tggtgacgat
     8701 attaccaggc tgggtccatt tttcgccgaa tttctcgata gcggcttttg gcaccggtga
     8761 agttgatggg tgaacaagta atttatagaa gtacggaacg ggttcactta aggtgacttc
     8821 taatgtgtga tcatcaatag ctttcacgcc gagatcggta atcggttttt tcccttcaag
     8881 aatttcatca ataccggcga tatgcccata ttgcagataa ctggcatacg gagaagcagt
     8941 gttcggatca acagaacgtt gccagctata cacaaagtct tgtgctgtga ctggcgtgcc
     9001 atcagaccat ttcgcatctt tacgcaaatg gaaggtccag actttcgcgt ctttattatc
     9061 ccaggattca gcgacgccag gtgctggatg accgtcaaga tcgctgacca gtaagccttc
     9121 aaacaggtct cggctgatat tagactccgg aacaccttca attttgtgcg gatctaatga
     9181 ctgaacttct gaaccattgt tacgtaccag tgtttgtttt tccgccagtg tgacgcctgc
     9241 gggtacatca gctgccagcg cgacattccc tgccattagc gcagccagaa cgccagctgc
     9301 tactaaactt ctcttggtga tgttggtcat tgttttttgg actccctcat tataattact
     9361 ggtattaacc agcatgtgta atcccggatt gggaccctgc acaggctcgg agactttctg
     9421 ctgtcaggtt actttatcgt tacgtattac tatcaccgac tttatttatt ctggtgattc
     9481 agacgatcac cttatgtcga ttcgtactgt gtctcccctg tcgagacaca ttattctttc
     9541 gcgacgctct ttaattgtta attattctca ttaccaataa agaatcgtct ggcggttgcc
     9601 cggaaagtac caaatgcggt tcttactcgc caatacattt tgcaaaattg ttaagcgatt
     9661 ctcttttatt gaccgtatca gaatctatag cttgcatcca tgccttaaca aacatctttc
     9721 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
     9781 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggtag
     9841 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
     9901 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
     9961 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
    10021 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
    10081 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
    10141 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
    10201 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
    10261 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
    10321 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg
    10381 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
    10441 atcgctatcg tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca
    10501 aaaagttgta gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
    10561 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
    10621 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
    10681 aagtaaacgg gaaaatcaaa aagggtctga atcacggtta gctccgaagc aaaagccgga
    10741 taatgttagc cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
    10801 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
    10861 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
    10921 aagcgatgct gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc
    10981 cagaagtgag taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta
    11041 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgaataagct tttgattttc
    11101 atcggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
    11161 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
    11221 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
    11281 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
    11341 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
    11401 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
    11461 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
    11521 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac
    11581 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc
    11641 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
    11701 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
    11761 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
    11821 cggtgtaggc gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc
    11881 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
    11941 gtctgttgtt gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg
    12001 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg
    12061 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
    12121 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
    12181 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
    12241 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
    12301 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
    12361 aacagctcgt atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa
    12421 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc
    12481 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
    12541 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
    12601 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
    12661 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
    12721 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
    12781 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
    12841 cgccgcgaag atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
    12901 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
    12961 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
    13021 tgtaactgac gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga
    13081 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
    13141 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
    13201 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
    13261 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
    13321 gggtatcgca tttgcgaacg ccttcctggg ggtatgtcac tcaatggcgc acaaactggg
    13381 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
    13441 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
    13501 ggctcgccgt cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg
    13561 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
    13621 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
    13681 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
    13741 gatctccgag ctgaaacaga ttctgctgga tacctactac ggtcgtgatt atgtagaagg
    13801 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
    13861 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttctgg
//
LOCUS       DAJTXY010000104        13787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTXY010000104 DAJTXY010000000
VERSION     DAJTXY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.104"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..454
                     /locus_tag="OO566_003127"
                     /pseudo
     CDS             1..454
                     /locus_tag="OO566_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            514..621
                     /locus_tag="OO566_003128"
     CDS             514..621
                     /locus_tag="OO566_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6606327.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="OO566_003129"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="OO566_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6606328.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="OO566_003130"
                     /pseudo
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="OO566_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="OO566_003131"
                     /pseudo
     CDS             complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="OO566_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="OO566_003132"
                     /pseudo
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="OO566_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="OO566_003133"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="OO566_003133"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6606329.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="OO566_003134"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="OO566_003134"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6606330.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="OO566_003135"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="OO566_003135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6606331.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="OO566_003136"
     CDS             complement(9943..10530)
                     /locus_tag="OO566_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6606332.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="OO566_003137"
     CDS             10700..11278
                     /locus_tag="OO566_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6606333.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12156)
                     /locus_tag="OO566_003138"
                     /pseudo
     CDS             complement(11395..12156)
                     /locus_tag="OO566_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="OO566_003139"
     CDS             complement(12209..13645)
                     /gene="mdtQ"
                     /locus_tag="OO566_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR6606334.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGMETDINASKTRQQLNDVVGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
BASE COUNT         3276 a         3871 c         3486 g         3154 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgca cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attagccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc cgccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg agcgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgcaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctatagc agcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc
     4981 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 cactaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac catcattttc ttgagcagat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat actcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaacgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctag cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtactctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgatgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccacgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccataccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatt
//
LOCUS       DAJTXY010000105        13775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTXY010000105 DAJTXY010000000
VERSION     DAJTXY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.105"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..171
                     /locus_tag="OO566_003140"
     CDS             <1..171
                     /locus_tag="OO566_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6606335.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="OO566_003141"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="OO566_003141"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6606336.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="OO566_003142"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="OO566_003142"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6606337.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO566_003143"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO566_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6606338.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIREHVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="OO566_003144"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="OO566_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6606339.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5430..6246
                     /locus_tag="OO566_003145"
                     /pseudo
     CDS             5430..6246
                     /locus_tag="OO566_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6250..7640)
                     /gene="tcyP"
                     /locus_tag="OO566_003146"
                     /pseudo
     CDS             complement(6250..7640)
                     /gene="tcyP"
                     /locus_tag="OO566_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(7773..8363)
                     /locus_tag="OO566_003147"
     CDS             complement(7773..8363)
                     /locus_tag="OO566_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6606340.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8526..9193)
                     /gene="hxpB"
                     /locus_tag="OO566_003148"
                     /pseudo
     CDS             complement(8526..9193)
                     /gene="hxpB"
                     /locus_tag="OO566_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            9340..9876
                     /locus_tag="OO566_003149"
     CDS             9340..9876
                     /locus_tag="OO566_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6606341.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9917..10777)
                     /locus_tag="OO566_003150"
     CDS             complement(9917..10777)
                     /locus_tag="OO566_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6606342.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPSFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10883..11173)
                     /gene="ghoS"
                     /locus_tag="OO566_003151"
     CDS             complement(10883..11173)
                     /gene="ghoS"
                     /locus_tag="OO566_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6606343.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11274..12203)
                     /gene="pfkB"
                     /locus_tag="OO566_003152"
     CDS             complement(11274..12203)
                     /gene="pfkB"
                     /locus_tag="OO566_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6606344.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12489..13247
                     /locus_tag="OO566_003153"
     CDS             12489..13247
                     /locus_tag="OO566_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6606345.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(13300..13407)
                     /locus_tag="OO566_003154"
     CDS             complement(13300..13407)
                     /locus_tag="OO566_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606346.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13579..>13775)
                     /locus_tag="OO566_003155"
     CDS             complement(13579..>13775)
                     /locus_tag="OO566_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606347.1"
                     /translation="FAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHKHYKTVETYYA
                     AINVISQSLSFSADEIKTYL"
BASE COUNT         3553 a         3167 c         3409 g         3646 t
ORIGIN      
        1 atccgtatta attttgccaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaaga
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagc atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca gggccttgag aatggcaaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgtgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgatccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagattc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcctg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagtgat
     5281 cttttttctt taattgttta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tggcttagat
     5401 ttaaataaga taataatttt gttaacaata tgaggcggga gatgttttct acaaacttaa
     5461 tacagagtaa ttatggtgat ttaaatatta aaagcctggc tttcgactct tttaaagaaa
     5521 ggcttgagtc taccatggcg gcactcactt tctttatatc aacgggtcaa tgtgactgcg
     5581 atgaaatagc agagtcgaac ttcaatcgta tgattgccta tatgtctaac attaactatg
     5641 atgcctcaaa gctaggagcg ccagcactta gttttgatac gtatctgcag gacaatgtta
     5701 aatatagagt gataattaat aatctttatg gcagtgagtt tcggataaga gatattaatg
     5761 aggattttgt tgggatggat tttacctgtg aattccgacc cgaaaaaatg acgaatttga
     5821 tcaatataaa aaataggttg gttattcatt atttaaggac tatatactat gaaaaaaatt
     5881 atatacatcc tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag
     5941 tttccttcga taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg
     6001 ggaatgggaa gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa
     6061 cgtttcgggt atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt
     6121 attgataagt tggacatttt tttcgctgac gatctgttta aatatgagtc agtatgcatc
     6181 gccgcgatgg aacatttaaa aagtaaagca acggccccta tacaaaacgg accgttgcca
     6241 gcataagaat taacggtgtg ccagttcggc gtcgtcttcg ctatccagaa tggctttatc
     6301 ggtttgcttc agccactggc tggtcagcgt gccagctgtc atcgagccac taacgtttag
     6361 cgccgtacgg cccatgtcga taagcggttc aacggagatt aacagcgcca ccagggttac
     6421 tggcaggccc atcgcaggca gcacaatcag tgcggcgaaa gttgcaccac caccgacacc
     6481 ggcaacgcct gcggaactaa cggtaacaat accgaccagc gtcgcaatcc acatcgggtc
     6541 cagcgggtta atgccaaccg taggcgcaac catcaccgcc agcattgccg gatacaaacc
     6601 ggcgcagccg ttctgaccaa tggttgcacc gaaagaggcg gcgaaactgg cgatggattc
     6661 aggaacgccc agacgacgcg tttgtgcttc cacattcagt gggatagacg cagcactgga
     6721 acggctggta aaggcaaacg tcagcacagg ccatacctta cggaagtact tcagcggact
     6781 cacgccatta atgcccagca gaatgccatg cacagcaaac ataatcagca gaccgaggta
     6841 ggacgcgaca acgaaacttc ccagtttgat gatgtcttgc aagttagaac ctgcaaccac
     6901 tttggtcatt agagccagaa cgccgtaagg ggtcaactgc atgacaaggc gaaccagttt
     6961 catcacccag ctttgtaggg tatcgatagc ggttaagacg cgttcacctt tcggcgcatc
     7021 atccttcagc agtttcaatg cagctacgcc gaggaatgcg gcaaaaatta ccacgctgat
     7081 aattgacgtc ggattggctc cggtaagatc ggcaaacggg tttttcggga taaaggacaa
     7141 gaccagctgc ggaacgctca gatcagagac tttaccaaca tagttacttt caatggcgtt
     7201 cagacgtgca gtttctgcac caccctgaac cagaccttca gccgtcaaac caaacaggtg
     7261 gtgaccagca caccgaccag cgccgcaatc agcgtggtaa acaaaagcgt accgatggtc
     7321 agaaaactga ttttgcctaa ctgagatgcg ttatgcagac gggcaaccgc gctcagaata
     7381 gaggcgaaga ctaacggcat aacgatcatt tgcagcagtt gaacattgcc gttaccaacg
     7441 atgttaaacc actgtacaga atctttaagt acctggctgt cagaaccata aatggtatgc
     7501 agggcaaggc caaaaaccac acccatcacc agacccacca gcactttttt cgccagactc
     7561 cactgtttat ggcgggtctg agccagcgca aacagcagta caacgaacac cacgatgttc
     7621 gcaattaatg gaaagttcat ccccgttctc ctgatcttat tatttctatc actgcgtagc
     7681 agtcatagtg ttggcgcaag attaacagaa ctgtgagctg gctcttatat tcaaatggaa
     7741 ttgcttattc cagaaaggtg atttttgcct ttttagtatt caacctgatg cttaataagc
     7801 cgatgaaact ggatctgcaa ggtattgatc atttgcgatg accaacgaac agcgctgttc
     7861 tcgggtaatg aatggggcat ccataccgac cagacaaata atgccatgca gataaaacgt
     7921 tccagttggt tgcccttttg gggaaccagg ataggcaagc ggaaacgcca gcggcatggc
     7981 cagagcaggg gaacaccggc gggtgtcagc atatcggcaa gtatgtggct caaataaccc
     8041 agcaccattc cttgtagcgc atcagccgga atgaaccagc cttccggaac cttaaggtaa
     8101 aaggttgcca gcagcgcaaa taccgccagc agactgtggg taaaaccacg atgcccaaaa
     8161 gcgcgggcga tcggttttga tatccatttt aatcgctgcc caagaaacga ctttgggtga
     8221 tcgatgtccg gtaacaaaca cgtcaggatt gcggaaggga caatatgcca ccagtcaccc
     8281 tgtgccagca cgggcgtcag ctcggcattt ttggcaaata ccgcacaagc aatagaaaag
     8341 agaaggtgac cttccgccgt catgataaaa ctcactaaac tgtcgatacg tacagtatag
     8401 gatttttata cagtgcacgg aagtggtgac ggtgtaactc tttctcttaa attgacgatt
     8461 aatccgtcag ttgaatgcaa attcagataa agaaccaggg gcagcgtgat cactgcccct
     8521 ggtcattaac cgagaaggtc ttttgcggtg agttctgtca gcgatgagag tttgacgttt
     8581 gctaatacaa aacgtggatc attttgcgct tctggcgcag gaacgacgat ggaacgcatg
     8641 caggctgctt tagaagcgat catgccattt accgagtctt ccagcgccac gcaggtcagt
     8701 gggtcaacgc ccagttttgc tgcgcagtcg agatatactt gcggatgcgg cttgctgtaa
     8761 ggcagttttt cagccgaggc gagggcatcg aaactgtcgc gtaagtcaaa catggtcaac
     8821 actttttcca gcatatgtag tggtgacgcg gaggccagtc ccaccaataa accttgttct
     8881 ttgcataacg caacagcttc gcgcacgcct ggtaataatg gacgtgtctc ttcaaccagt
     8941 gaaatggcac gggcaataac ccgttctact acttcctgac ggcttggccc attccacggt
     9001 tgccgggcgt acaaagatcg accaccatat cgatgcgtaa acctaaggtg tccggcagct
     9061 cgttacgacg ggagatatct acccccaggc ttgccatcac atccagttcg gctcgatccc
     9121 ataaaggttc tgagtcgata agtaatccat ccatatcaaa aattgcagca agaatctgac
     9181 gcggggttga cattacaacc tctcctttag agcattatgt gtaaggtatt aagccgtgct
     9241 tatgattaag cgcctatcat atcgccttta aagaatgggc gaaaacagat atctacaggt
     9301 aaactaagtg aaacgacgcg tcttaatcaa ggagaactca tgacgtatca acaagctgga
     9361 cgcattgctg ttttgaaaag gattttgggt tgggtgattt ttatacctgc attgatctct
     9421 acattgattt ctttgctgaa atttatgaat acccggcagg aaaaccagga aggcattaat
     9481 gcggtcatgc tcgactttgc tcatgtcatg atcgatatga tgcaggcgaa tacgcctttt
     9541 ttgaatctgt tctggtataa ctccccgacc cctaatttta atggtggtgt gaacgttatg
     9601 ttctgggtga ttttcatcct gatttttgtc ggactggcgt tgcaggattc cggtgcccgg
     9661 atgagtcgcc aggcgcgctt cttgcgggaa ggtgttgaag atcaactcat tctggaaaaa
     9721 gccaaagggg aagaggggct aacgcgtgaa caaattgagt cccgtattgt tgttccgcac
     9781 catactattt tcctgcagtt tttctcacta tatatcctgc cggttatttg tattgctgca
     9841 ggttatgtgt tcttttctct gcttgggttt atttaatcaa aaggccgcac ggagcggcca
     9901 ttatcctcaa cccatatcat gctgctaata atctatccaa tgactgctga gcaataacca
     9961 aatgctgacc gccaaataat cttgcacgat ttaacagcgt gtagagttgg taaaccggtt
    10021 gacgttcaag gaaatcggca ggtagcgggg atactgactg atagccgtca tagatttgtg
    10081 gcggctgttc agtatgcagc ggtaacatcg ccaggtcgca ctctcggtca ccccagtagc
    10141 aggcagggtc gaaaatgtac gggccatccg gacccagcgc acagttgccg gaccataaat
    10201 cgccgtgcaa cagagaaggc tgcggttgat gcgaggccag acgctgctga atatgctcga
    10261 cgatggcgtc gatattgccg aaagcgatcc ctttctctgc tgccagttcc aactgccagc
    10321 caatccgttg ttcagcaaaa aacgttgacc agcgacgttg ccaggtgttg ggctgtggag
    10381 ttgtggagag cgagttatca aaatcgaggc caaattgtgg ttggtcactc cattgatgta
    10441 aacgcgcaat ttgctgacca agaataaatg cgctatgcgc atccagcgga cgaggtggga
    10501 gataatccat caccagaaaa ctgtagtcac ggtcagcgcc tactgcccaa accttaggca
    10561 cggtgacggt tttactacgc gacagtaact ccagttggtc ggcttcggcg gtaaaactgg
    10621 gaagcagttc cctttcatca catttgacga aaaagtcatg tcctgcatag cgcaaatgcc
    10681 atgcggcatg gacttctccg ccaggcagtt cattacgcag ttcgatttcg ccttcaccta
    10741 actgctcgct caaaagacga ctgattgcct gccacatgat gtctctccca tgttgtctgc
    10801 cagatcataa ggttagcgca agaatgcggt gtaaaaacac gagctaacgc acaccacaga
    10861 ctgattattg cccaaaaacc atttattttc tgttgctatt ccattcctcc caggtcgtga
    10921 tggtgatttc aggatctttg ccggttgcac tttgggtaag ccccgaaacc agttctctaa
    10981 tttcttcttc actttgggta ctgataagtc caaaagtgtt agtccccagc tcatggatat
    11041 ttccctcatc atccgtcatg gtgagcaaaa aaccgtcgcg agtcaggtaa ttattcaact
    11101 cgttaatttc agtcaatgta tcctcatgca acatgacggt tatgacataa cggacaagat
    11161 cgccactggc catagttcac ccctttgtta tcacatgact tttgagcata gtcggagaaa
    11221 cgcgttgccg acaggttggt ggtgattccc ccaatgctgg gggaatgttt ttgttagcgg
    11281 gaaaggtaag cgtaaatttt ttgcgtatcg tcatgggagc acagacgtgt tccctgattg
    11341 agtgtggctg cactccccgc agctacgcca aaacggacca tctcttcaag agaggcattt
    11401 tctgccagtt tcagtgtcat cgcgccgacc atgctgtcac cagcgccaac ggtactctgg
    11461 cttttcaccg gtggtggcac cacctgaatg cagttttcac tatcaacacc cagcgctcct
    11521 tgtggaccaa gggaaacgac aacccgtttg gccttgccgc tattaacgat ttcctgcgcg
    11581 gctttgcgga catcgtctgg ctgggtgagt tcgcgactca ccagcgcact gagttctttt
    11641 tggttaggct taaccaactc gatgttacca atcgccagtg ctgcacttaa cgcttcgcca
    11701 gaactgtcga tgatgcagcg gatcccttgg ttttgcgcag cggaaatcag ttgggttaat
    11761 ttttccagct tcacacctgg cggcaggctt ccgcttatga ccaggatggc cccggattca
    11821 atttccagaa cttgctcttc aagctggcga aactcatctt catttaatgc cgcgcctggc
    11881 ataacaaaac gatactgttc accgcttgct tccacatgta cgtgtaaatt ctgccgggtc
    11941 cagtctttgg cttctacagt agcgacgggg acattttcat ccgccaacag tgaaaccagg
    12001 tgttcgccgg tcgcgccacc cgccgggaag atcgctgtgg cactgcctcc aagatgggca
    12061 atggcgcggg cgacgttgat gccgccgccc ccgggttcga acaccggtgc ggtacagcgc
    12121 agttttcctt cgggataaat ttgcggggta attgttgcgc tatcgagaga gggcgcaagt
    12181 gtcaacgtat agatacgtac catcatttcc tcctataggc tgatttcagt ctggcaccga
    12241 atcagcggaa agtgaagaaa ttaacaatat gatttattag agctttattt aaaattttgc
    12301 agataaatat atataaataa aaatctgttt tcgccacatg cttattcaaa aaatgaaata
    12361 agaatacatt gctaagttat tcgcgccttt ttataattcg ttacctttcc agggacgcta
    12421 atctttgatc cttaagaaag tttccgggac cgtgatggtc tcttttttgt tgtacggact
    12481 cttgataaat gaagcttttg aagacagttc ctgccatcgt tatgttggcg gggggcatgt
    12541 ttgcgtcact gaatgcagct gccgatgatt ccgtttttac tgtcatggac gatcctgcca
    12601 gcgctaaaaa acctttcgaa ggtaatctga acgcaggata tcttgcacaa tcaggcaata
    12661 cgaaaagctc ctcacttacg gctgacacca ccatgacctg gtatggacaa accactgcct
    12721 ggtcgctgtg ggggaacgcc agtaatactt cttctaatga tgagcgttct tcagaaaaat
    12781 atgcggcggg cgggcgtagc cgctttaatt taactgatta tgactattta tttggtcagg
    12841 caagctggct gacagaccgt tataacggct atcgagagcg cgatgtgttg accgcgggtt
    12901 atggtcgtca gtttttgaat ggcccggtac acagtttccg ctttgaattc ggtccaggtg
    12961 tgcgttacga caaatatacc gacaatgcca gtgaaaccca gccgctgggc tatgcctccg
    13021 gggcttatgc atggcagttg actgacaacg ctaaatttac gcagggtgtt tcggtctttg
    13081 gcgcggaaga cacaacgctg aactccgaaa gcgcattaaa tgttgctatt aatgaacatt
    13141 ttggactgaa ggtggcttac aacgtcacat ggaactctga acctccagag tctgcaccgg
    13201 aacataccga ccgtcggaca accttatcgc tgggctacag catgtaaaat tatctgccgg
    13261 accgtgttat gcggtctggc tgtcttcgtt tgtcactgct cagccccaaa tcattgctgc
    13321 ccaacgtagc aacaacatag cgccacagat tgccagtagc accaccacca tcttccagtg
    13381 ttttttatca aagcgtttag tcggcatacc agtgtcctgc atatctcttg tgcgtgataa
    13441 cggagtgtaa caaaagggac aggcatcaag ggattttttg ttattacagc cgtcgggtgg
    13501 cgttgtgcac atggtcactg tttctgcgtt ttatgaaaaa aataaaatta tgtatcgata
    13561 tatatcgtaa atagctggtt ataaatacgt ctttatttca tcagcactaa aactcaggct
    13621 ttggctgatg acatttatag cagcataata tgtttctaca gttttataat gcttatgatg
    13681 aatggctata tgaacagctg acatgttgtc atgattttta gcggccaata gtgtaaataa
    13741 ctgatgttga ctaaaagaat gtttttttgc aaaag
//
LOCUS       DAJTXY010000106        13767 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTXY010000106 DAJTXY010000000
VERSION     DAJTXY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.106"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(184..1437)
                     /locus_tag="OO566_003156"
                     /pseudo
     CDS             complement(184..1437)
                     /locus_tag="OO566_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1544..2437
                     /locus_tag="OO566_003157"
     CDS             1544..2437
                     /locus_tag="OO566_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6606348.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2572..3792
                     /gene="mlc"
                     /locus_tag="OO566_003158"
                     /gene_synonym="dgsA"
     CDS             2572..3792
                     /gene="mlc"
                     /locus_tag="OO566_003158"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6606349.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3917..4612
                     /gene="bioD"
                     /locus_tag="OO566_003159"
     CDS             3917..4612
                     /gene="bioD"
                     /locus_tag="OO566_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6606350.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4565..5857)
                     /gene="clcB"
                     /locus_tag="OO566_003160"
     CDS             complement(4565..5857)
                     /gene="clcB"
                     /locus_tag="OO566_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6606351.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6016..6630)
                     /gene="dmsD"
                     /locus_tag="OO566_003161"
     CDS             complement(6016..6630)
                     /gene="dmsD"
                     /locus_tag="OO566_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6606352.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6673..7527)
                     /locus_tag="OO566_003162"
     CDS             complement(6673..7527)
                     /locus_tag="OO566_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6606353.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7529..8146)
                     /gene="dmsB"
                     /locus_tag="OO566_003163"
     CDS             complement(7529..8146)
                     /gene="dmsB"
                     /locus_tag="OO566_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6606354.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8157..10427)
                     /locus_tag="OO566_003164"
                     /pseudo
     CDS             complement(8157..10427)
                     /locus_tag="OO566_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(10436..11102)
                     /locus_tag="OO566_003165"
                     /pseudo
     CDS             complement(10436..11102)
                     /locus_tag="OO566_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            11085..12685
                     /locus_tag="OO566_003166"
                     /pseudo
     CDS             11085..12685
                     /locus_tag="OO566_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            13179..13547
                     /locus_tag="OO566_003167"
     CDS             13179..13547
                     /locus_tag="OO566_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606355.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
BASE COUNT         3448 a         3511 c         3519 g         3289 t
ORIGIN      
        1 gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt acttataatc
       61 ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt aggatggcta
      121 ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt ccggacttat
      181 ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa tgaccagcat
      241 cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa caccacccag
      301 cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg aggcctggcc
      361 tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg ctgcgaagaa
      421 tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa ataaggtcat
      481 cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc gcccatagcg
      541 ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag cccgcgataa
      601 taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat agccgatgta
      661 attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca ataacggtaa
      721 tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc gcagcgaagt
      781 cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgccg aggccagcgc
      841 gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga agacaccact
      901 aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc ccatcaaaaa
      961 ggccacgaaa ctggggggga tttcctcgct aagataagtc atgccaacag ctgccacgcc
     1021 acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc tggtcatcat
     1081 tgtcgaaagt aacgtacaaa tggaggctaa cagtagcgcc gtgaccatca ctggtttgcg
     1141 accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca tcgccgtgga
     1201 aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg aaagcaccgg
     1261 aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag agaacagcgc
     1321 cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg gctgagaaat
     1381 gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac ggctcacttg
     1441 aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa aatcgaattg
     1501 ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata ttgaacttcg
     1561 tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg cggctgcccg
     1621 cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc aacaaatcgg
     1681 tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag gaaaacagtt
     1741 tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc gtgctgaaag
     1801 actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg ctccttttat
     1861 tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcctgatg tccatttaca
     1921 aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa cgctggatat
     1981 gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca tcgtccatga
     2041 accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga gtgtaacact
     2101 ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga ctgggctgta
     2161 tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca ctcaggaggt
     2221 gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt caattttgcc
     2281 tgcgtcattt aaacgtgttc agctcaatga aatgcgctgg gtgccgattg ctgaagagga
     2341 tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc cagctgcgcg
     2401 taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc gaaaaagccc
     2461 gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta ttgaagtgct
     2521 tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc ggtggttgct
     2581 gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc ggtttatcgc
     2641 ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc gcagctggct
     2701 cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt gcgagagctg
     2761 gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt tgaaactgaa
     2821 gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc tctgcgcgat
     2881 ctgagcagca aactggtggt ggaagagtcg caggaattgg cgttaaaaga tgacttgcca
     2941 ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca gaaaaaactt
     3001 gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga aaatggtatt
     3061 gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga ggcgctggag
     3121 cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac gatggcagag
     3181 gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat cgatcacaac
     3241 gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag tagtctcgtg
     3301 gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg gaaccacggc
     3361 tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct gcgtctcaat
     3421 caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt gtgtcaggcg
     3481 gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc gcatgtaggg
     3541 cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat tggctcaccg
     3601 ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg tcagcaggcc
     3661 cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa ccagggcacg
     3721 atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt gattcgtctg
     3781 ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca atttgggcca
     3841 ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt tgtgaagcat
     3901 aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca cttctgtagg
     3961 gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa aaacggttgc
     4021 gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc gtaataaaga
     4081 tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag ttaatcctat
     4141 cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt acaccctcat
     4201 ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag aagggactgg
     4261 cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag tgcaggaaca
     4321 actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg cactgctaac
     4381 agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta accgaatcaa
     4441 cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac ttccggcacc
     4501 gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg ggcaatacat
     4561 ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt aacgtccgcg
     4621 aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga tactccccgg
     4681 tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc gccaacagtg
     4741 tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg aaccataatc
     4801 ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc ggtgtaaaga
     4861 caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta caaaggaaga
     4921 tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg gtgctatagc
     4981 cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac ccgcctagtg
     5041 ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag gcgttcatta
     5101 acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata atcagcgcat
     5161 agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg tcgctatgat
     5221 taatcagatt gctaaccagc aatgccacga cggcggaaat aatcaccggg ccgagagagg
     5281 ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca agcggggcac
     5341 gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc cattcctggc
     5401 gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga atcatcgcac
     5461 cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc gatttaacca
     5521 ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa tcggtcggcg
     5581 catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc gccgccagtc
     5641 cgccgagcgc cggagttagc agccgtcgcc agggggaaag gtttgtcgct gcattgacca
     5701 gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca tgacgaaacc
     5761 cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga cggcggaaca
     5821 tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg caatgaaaca
     5881 cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc tttaagagat
     5941 aaaaaaacgt gacattgtaa cgtattattg ccgaatcgcg cgtaatcgtc accatccggc
     6001 aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca acagttgtga
     6061 ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga aagggtgttc
     6121 tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt gccatgccag
     6181 caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca tcaacagcag
     6241 tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc ctttctcgcg
     6301 catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc gatccagcca
     6361 gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac gctgccaggc
     6421 ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg ctaatgacgc
     6481 ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa ccagaggtgc
     6541 ggcttccgca ctctctggag cgtaataaaa cagcgctccc aacacgcgcg ccgcgacaga
     6601 aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg cgggttgccc
     6661 cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc cataaaagag
     6721 cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa gtccggcaac
     6781 gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg cgcataacaa
     6841 taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg cttgttgcac
     6901 ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg cacaagcgat
     6961 cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc gggctgcccg
     7021 cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga aagccagcgt
     7081 agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg tcatcatcca
     7141 gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca acgcttgcgg
     7201 cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa atatggaacc
     7261 ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt taaacgctcg
     7321 cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca acagccagag
     7381 gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg cgaaccagcc
     7441 aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa agataaccag
     7501 tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc agataaccag
     7561 tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg ggttttgtga
     7621 aatgcgcgcg cggcagtggc gcgacggcag ccagagtacc gtgcttctgg cgcagctctt
     7681 caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg ggttgtttcc
     7741 cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt tcagcattgt
     7801 actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata caaacatctt
     7861 catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg cagacttttg
     7921 tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg aacacgttct
     7981 ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa atacggcgga
     8041 aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag gttttacagc
     8101 cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc tactccttaa
     8161 accttttcga tctggacaag attgctgtgc gacgggtttc cctttgctag cggtgaaggg
     8221 cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc aaacatatcc
     8281 gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg cggagtcact
     8341 tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc gccatgacgg
     8401 ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca ggcctgctgt
     8461 aacacatcaa tattgccgta gctggagtgg gtacgcgctt tgtagtggaa gcccgttaac
     8521 tgcagtggat aggttttccg caggggagcg tcccagccat caaaacctga ggtatacgca
     8581 ggaatgggat gaataatttc atcttttttc aattcccagg tatctgcaat cttcgccagt
     8641 cgttcagaat aaatttcgat tttccccgaa ggtgttttca acgggtttgc ctgtggatct
     8701 tcacggaatg cgcggaaagc gacgtagtgt tcttccgggc attttttctt aaagatcccg
     8761 gtcgttttca tctcctcgta gtcgggcatc tcagggttac gttccttcgt tttcgcatgg
     8821 agatatttga tccattcatg ctgactgcga ccttcagtaa aggtttgata aacgtctggt
     8881 cctaagcgtt tggcgacttc actcagcatc cagtagatgg gtttgcgttc aaattttgct
     8941 gaggttgcgg gttgggcgag gatcacatag cccatattcc ctgcagattc atgagagata
     9001 aggtcttctt gctctgttgg catcaggtca ggcaacagga tatcgcaata cttagccgag
     9061 gccgtcatga agtggtcaat gccaacaatc atctcgcact tgctgtcatc ctgaagcacc
     9121 tcatgggtgt gattgatgtc gccatgttga ttgatcaatg tgttactggc gtagcaccat
     9181 aaaaacttga tggggacatc cagtttttct tttccacgaa caccatcacg ggtcgcggtc
     9241 attttcgtac catggtcgat ggcatctgtc catgtaaaaa cggaaatctg cgttttaaca
     9301 ggattctcga gcatcgggaa ccattctacc cccagatccc agctaccttt gcgtacacct
     9361 gagttgccgc cgtttatgcc gacgttaccg gtaagaacgg aaagcatggc aatagcgcgg
     9421 gacgtttgtt cgccgttgga gtgtcgttgt ggcccccaac cctgacaaat ataagcaggt
     9481 tttgctgaac cgatctctcg tgccaactgg ataatttttt ctgccgggat gctggtgatt
     9541 tttgctgccc attccggcgt tttagctatg ccgtcaggcc cttcgcccag aatataggct
     9601 ttataatgcg cgttacgtgg tgcgttggcg ggcagcgttt tttcatcgta accaacacaa
     9661 tatttgtcga gaaatggctg atcgaccatg ttttcagtaa tcagtaccca ggcaatcgca
     9721 caggccagtg cgccatcggt gccagggcga atgggcagcc attcatcttc acgccctgca
     9781 gctgtgtcgt tatagcgtgg atcgatgacg atcatgcgtg cgtttgaacg ttcgagggct
     9841 tgctcgacgt agtaagtgac accaccgccg ctcatccgcg tttctgccgg gttatttccg
     9901 aacataacga ccagtttcgt attggcgata tcatccgggc tgttgccatc attggcaccg
     9961 aacatataac tcattgcggc actgatctgt gcggtactgt agctgccata gcgactgaga
    10021 aaacccccgc aagagttcat cagacggtac gggacgttcg agttggtgat atttccgcca
    10081 tctacgcctg ttccgtacag gacatgtaca gcctcattgc cgtaatcttt caggatccgc
    10141 cgaagattat cactgatggt atccagggct tcgtcccaac ttatccgttc aaatttacct
    10201 tcaccgcgct cgccgacgcg cttcatggga tatttcaacc tgtcaggatg attcatccgt
    10261 cggcggatag agcgcccgcg taaacatgcc cgaacctgat gattaccgta aacgtcgtca
    10321 cctgtcgtat cagactccac ccagtacacg gtgtcatctt tcacatgcaa acgtagcaga
    10381 cagcggctcc cgcagttaac ggtgcaggaa ctccagaccg ttttctcttc tacccatctt
    10441 tttccagttc ccaggtacgg gcaatttccg ccagcttgct ggaataaatt tcaatcttac
    10501 cggaaggcgt tttaagtgga tttgcctcgg ggtcgtcacg aaatgctttg taggcgacaa
    10561 aatggccatt gggatcttta cgcttataga tacccatttt tttcagttcg tcgtaagacg
    10621 gtaacgccgg atctttggca agcattttgg cgtacagatg ttgtaaccat tgttcctgcg
    10681 tgcgaccttc tgtgaacttt tgatagacgt caggtccaag acgtttcgcg acttcactca
    10741 ggatccagta aatcggttta cgttcgaatt tttcgctggt gactggctgg aggaaaatga
    10801 gatatcccat gttaccggcg tagtcgttag gaataatatc ttcctgctca acggtcatca
    10861 ggtctgccag cagaatgtcg gcatattttg ccgtatgagg tcataaagtt ttcgatgacc
    10921 acaatcattt cgcatttcga ttcgtcctgc agaatttcat gcgttttgtt gatgtcagaa
    10981 tgctgattaa cgagggtatt tcccgcgtag ttccagatgg actccactgt cagacggatg
    11041 gatggacttg aagtagttga ctcaacgcta cttaaggtcc tgatatgact gacattaacc
    11101 atgataatac tgtatatgca tccagttctt tctctgggtc tccctatctg tatcagcaag
    11161 tctacacctc aagtcctgac acaaggctga cacaaatcaa ggaccttgca gtcccgcact
    11221 tgctcgcaag gctcgctacg ccacagttag tccgtagagc taacggtcga tatactatcc
    11281 gcttcagact caagggacag accacaccgt ttctttcagt atcgacccgc agcacagaca
    11341 ggagagtagc cacaatgcgt cagagagagc ttgcagccac cgctaaggcc ttcatgttgg
    11401 ataggcctga agtctcacta caggagctta cagagcatct ccggtctatg gctgagcagt
    11461 tcctgacaga cgctagtgac gattactgga atggacttga ggtcgctact ttggtggacg
    11521 agaagtccaa cctgagggag ctagccgcta cgcaagcctt gagtctggac cagcagaagg
    11581 gcataaggtt agcgcttgaa gtcctgaccg cagcacagca gagggtagac actggtgata
    11641 cctctggtct gattaagctg attgatgata ataaccatac tgattactca actataggcg
    11701 actctacgtc aattctgaag aatgagcagg gggatagcca gcagttttca cacaggagcg
    11761 ccaaccatct gtagtttttt caagcctagt gtcctcgctg ctggctgaga aggcccagac
    11821 cctgaagtca tccagctaca aggacctgtc gtcctcactg aataccgtct ctcggttctt
    11881 acctgaagac atggacctga tgtcccgctc tgagtggctg gctgtcaggg acgctatgct
    11941 ggcttctgag gtgagaccgt cgacgattaa caagctgctg actaaggcga agatgtgtct
    12001 tgattacggt ctgatgaatg ggcaacttga gggccgcaac cctattgaac ggatgaagct
    12061 gaccaaggat atcgactcca agcgcagagc gttcacagac gaggagctgg agagacttct
    12121 ggtccgtgtt gaggctgagt atcagttcac acgacacacg gctcacacga cctctgaggc
    12181 ccgtagatgg gcgtctctgg tttctgtagt tactggcgct cggtctgctg aggtctgcca
    12241 cctgactaaa cgtgacattg taaccttaga caatggactg gtctgtatcg atataaacga
    12301 agatggggac ggtaagtctg tgaagaacaa gcattcagtc cgacttgtgc cgctgaccga
    12361 tggcgttcat gggttcgacc ttgagtcttt cctcaaatgg gtagacatgc agcctgatga
    12421 tggtcctctc ttcgggatga ctccgagcgc ctactctagc tggtttaact caagggtcct
    12481 gactgaggcc ctaggagact ctcaggatgt ctctctccac agtctgagac attggctggc
    12541 gacccgaatg aaagagcgtg gagtcaatct ggtggacgct caaggcatct taggtcacag
    12601 cagtcagtca attacgtatg acctgtacgg gaaaggacac gctgtgggta ggctggctga
    12661 tgtgcttaag acagctttac tttaagtagg gagacccaag gtggagacca tatacggctt
    12721 gatggttgag accttgggtc actgaagtct gactgaagtt agggagggtg ataataatca
    12781 taatccctcc tgactatagg cccactggtc cgccttcggc tgtccgactc taagtctgtc
    12841 tgtggtctta tttaaactgc gtcctaaact ccagtccctc caaattccta tggtctcctc
    12901 ttgacattcc tgtttaactg tgagtcgcct ctggtctctc tttatatata cttccttaat
    12961 tcctgaggcg tctttagtcc ttgacatttg gttcaacctg tgagtcacgt ccggtccccc
    13021 tctttatatt tacttcctta attcctaggg tcgccttaat ccgtgacatc cagtccagcc
    13081 tgtgagtcac atcctgtcgc gctactgtat gcatcaccag tgatggactt gattatcact
    13141 ataggggaac caactaattc aactacagga gatactctat ggcacgacca atcgcaactc
    13201 acgacaacac gttcaccaaa gcctacttgc agcagcactg cggggacctg ctgtcattcg
    13261 atggtcaggg cgacctgagt ggttggctgg atgatgtcct tactggtgct ggtcgcctga
    13321 acgaatcaat ggcaagcaat accaagcctg tcagcccata cttgattctg acccaactac
    13381 taacgcacga cacattaact gtaagcgctg tgcaggagtc cctgagccgt aagcgtgttg
    13441 ccttgggtga acctatggtt tcaactcgct acgctcgcta cgtctatgct accgtcgtca
    13501 gcgccagtaa atccgtccag taccacgcca gtaaagctgg ttcatgatta cggaccctaa
    13561 actatcacta tagggagacc agcctccgcc tctgagtctg atgcgtcaga acatgcgagc
    13621 atctggtcaa ccgacattaa gtatctctcc taagtaatca aggcatgtga ccgcctctgt
    13681 cagacctcgt gtatcgacct ccagctcgtt agcgttcttc agtcatctca gttagtagct
    13741 cgtctactca ctacattggc ttaagtc
//
LOCUS       DAJTXY010000107        13655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTXY010000107 DAJTXY010000000
VERSION     DAJTXY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.107"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..387)
                     /locus_tag="OO566_003168"
     CDS             complement(<1..387)
                     /locus_tag="OO566_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6606356.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNK"
     gene            complement(418..1110)
                     /gene="fimC"
                     /locus_tag="OO566_003169"
     CDS             complement(418..1110)
                     /gene="fimC"
                     /locus_tag="OO566_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6606357.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1311..1853)
                     /gene="fimA"
                     /locus_tag="OO566_003170"
     CDS             complement(1311..1853)
                     /gene="fimA"
                     /locus_tag="OO566_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6606358.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2334..3200
                     /gene="folD"
                     /locus_tag="OO566_003171"
     CDS             2334..3200
                     /gene="folD"
                     /locus_tag="OO566_003171"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6606359.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3202..3414
                     /gene="ybcJ"
                     /locus_tag="OO566_003172"
     CDS             3202..3414
                     /gene="ybcJ"
                     /locus_tag="OO566_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6606360.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3522..4043
                     /locus_tag="OO566_003173"
     CDS             3522..4043
                     /locus_tag="OO566_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6606361.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4079..5464)
                     /gene="cysS"
                     /locus_tag="OO566_003174"
     CDS             complement(4079..5464)
                     /gene="cysS"
                     /locus_tag="OO566_003174"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6606362.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5638..6132
                     /gene="ppiB"
                     /locus_tag="OO566_003175"
     CDS             5638..6132
                     /gene="ppiB"
                     /locus_tag="OO566_003175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6606363.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6135..6857
                     /gene="lpxH"
                     /locus_tag="OO566_003176"
     CDS             6135..6857
                     /gene="lpxH"
                     /locus_tag="OO566_003176"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6606364.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            6975..7484
                     /gene="purE"
                     /locus_tag="OO566_003177"
     CDS             6975..7484
                     /gene="purE"
                     /locus_tag="OO566_003177"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6606365.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7481..8548
                     /gene="purK"
                     /locus_tag="OO566_003178"
     CDS             7481..8548
                     /gene="purK"
                     /locus_tag="OO566_003178"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6606366.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8685..9578)
                     /locus_tag="OO566_003179"
     CDS             complement(8685..9578)
                     /locus_tag="OO566_003179"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6606367.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRWVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9575..10222)
                     /locus_tag="OO566_003180"
                     /pseudo
     CDS             complement(9575..10222)
                     /locus_tag="OO566_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10225..11457
                     /gene="allC"
                     /locus_tag="OO566_003181"
     CDS             10225..11457
                     /gene="allC"
                     /locus_tag="OO566_003181"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6606368.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11468..12253
                     /gene="allE"
                     /locus_tag="OO566_003182"
     CDS             11468..12253
                     /gene="allE"
                     /locus_tag="OO566_003182"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR6606369.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(12481..13626)
                     /gene="glxK"
                     /locus_tag="OO566_003183"
     CDS             complement(12481..13626)
                     /gene="glxK"
                     /locus_tag="OO566_003183"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HCR6606370.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDALFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
BASE COUNT         3287 a         3620 c         3427 g         3321 t
ORIGIN      
        1 tttattgttt tgctgctggg ctaactcggg aaatgcactg ctattaaggc caattcgttc
       61 aaggagtacc tggttaaaac agggcattaa cccacctgat ttatcacctg tattttctgt
      121 tgtcgattca aatacgatat cctgtgaacc aatgaactca tcattacgcc agagatccac
      181 ccgataaacg cccgcaggtt gatgattacc tctctcaaag cgcgataaat cagcaacgga
      241 tgtgccattc tctaataaaa atgccgggtt gaaataactt tccgcatagc ttaaaaagga
      301 gggtaaaatg gcaagagatg aataacaaat tccggccaga caccaggtat acctctgcgg
      361 aatatccgta gtagtgggta ttttcataat tagctctgat attgtcagat gattcggtta
      421 gtttaagttc acttctctga ccggggttac tgaaccataa tcattaacgg tctgaaattt
      481 cagctttccc tgcactcctg agggtaaggg aatttgtgaa ctaactctgg gtgccgccat
      541 agcattcccc aacttttggc tgcccacttg caggttaacg agggtgacat aaaaaggtga
      601 tggattatga acattgatat agttaccgga tcgcgaaaac ttgagtgtgt ccggcgcttc
      661 tgcgggtaat tcttgtaatt gaattggacg gagaaataat ttcatgcgcg ataaaatcgc
      721 cagttgcaaa acattcctgc cgttcaatgc atttttatct accgaaggga tcgtcttaac
      781 attcatccag aacagagact cacgatctgc cgccagcggt ggaccggtgt aaataatacg
      841 caaagtattt tcgcttttgg gttcactaac aaatagtggc ggtgtaatga tgaatgactt
      901 ttcttttaca ccgctgctgt tttcaatcca cgaattgacc agaaagagct cattggtatg
      961 gctattttta atccataccg cagtctgttt agcatcagcg ggataaataa tacgcgtggc
     1021 acctaatgcg atccctccgg cagcatgggc gctggccgaa atgattaaat aaaatatcat
     1081 gagcatcaat aactttattt tagtcatcat agttaatacg ccttgtgtat tcctgacggc
     1141 aaaagattgc tttggcttct tacaacacat cattcccttt tcatttaata aagcggcatg
     1201 atacaaagtg aacgtggttt cgatttacat tgccaccata ccgcttcaga caaccggtac
     1261 attactttgt tataagacgt gatatataat tcaaaacaac gtggttttga ttattcgtat
     1321 ctcataataa aagtcgcgtc agcatttgct tgccctgctg atgcacttgt accggtgcct
     1381 ttataacgtg cagaaaaatg aagaacgttg gtcccgggga tcaagttctg gttggttgag
     1441 aagctattcc catcaggttt gagaattgcg gatgtattat caagtatttc aataccgaca
     1501 cccgttgctg ttgttgtatt tgtactggat gcaatggcca ataaattatc attgattgca
     1561 tcagcctgac cagaaaatgc aacggcagca ttagcggcaa caacaggatc gcagtcgttc
     1621 aactgaatgg tgaatggaat taatgcagag gtattaccaa cagcattgaa aatatcggta
     1681 cgatattgac cgagtgtgac aacctggtct gccgagtcag tattcactga acaggcagca
     1741 ttcaccagtt cgccttcaaa gtgaattgta ccgccatcta caacggcagc ataactaccc
     1801 gtagcggcga atagtgcggc agccagcgca gacgaaataa atcttaattt catatatatt
     1861 ccttcaatct catttatcga ctccacatcc gtatataacc gattacttta tttaagacac
     1921 tgatagtagt aaaattcctt tttatcctct aagaatgtct taattgaaaa tatgcactct
     1981 attctaaaaa atagagagcc ccgttagatg aatacttccg cgcaaaatat attcaacaca
     2041 aatatagacc tgaagcggta aattaccagg ctgaaaattc tttttatatt gtcaggtatt
     2101 tcttaaatta tcttaatcct taaacaagga aataaatcag ttccagattt acaacgccat
     2161 catgtacgaa aaatgaagct ttcaatctca gcgacctcag cgacggtgcg cctaaccttc
     2221 gcaagaggtc gcttcacgcg ataaatctga aacgaaacct gacagcgcgc cccgcttctg
     2281 acaaaataga cgcatcccct tcgacctacg taacagatgg aatcctctct ctgatggcag
     2341 caaagattat tgacggtaaa acgattgcgc agcaggtgcg ctctgaagtt gctcaaaaag
     2401 ttcaggctcg tattgcagcc ggactgcggg caccaggact ggccgttgtg ctggtgggta
     2461 gtaaccctgc atcgcaaatt tatgtcgcaa gcaaacgcaa ggcttgtgaa gaagtcgggt
     2521 tcgtctcccg ctcttatgac ctcccggaaa ccaccagcga agcggagctg ctggagctta
     2581 tcgatgcgct gaatgccgac aacaccatcg atggcattct ggttcaactg ccgttaccgg
     2641 cgggtattga taacgtcaaa gtgctggaac gtattcatcc ggacaaagac gtggacggtt
     2701 tccatcctta caacgtcggt cgtctgtgcc agcgcgcgcc gcgtctgcgt ccctgcaccc
     2761 cgcgcggtat cgtcacgctg cttgagcgtt acaacattga taccttcggc ctcaacgccg
     2821 tggtgattgg cgcatcgaat atcgtcggtc gcccaatgag catggaactg ttgctcgcag
     2881 gttgcaccac caccgtgact caccgcttca ctaaaaatct gcgtcatcac gtagaaaatg
     2941 ccgatctgtt gatcgttgcc gttggcaagc caggctttat tcccggtgac tggatcaaag
     3001 aaggcgcaat tgtgattgat gtcggcatca accgtctgga aaatggcaaa gttgtgggcg
     3061 acgtcgtgtt tgaagacgcg gctaaacgcg cctcatacat tacgcctgtt cccggcggcg
     3121 ttggcccgat gacggttgcc acgctgattg aaaacacgct acaggcgtgc gttgaatatc
     3181 atgatccaca gggtgagtaa catggcgaca ttttctttag gtaaacatcc gcacgttgag
     3241 ctgtgcgact tgctgaaact ggaaggctgg agcgaaagcg gcgcgcaggc gaaaatcgcg
     3301 attgccgaag gccaggtgaa agtcgacggt gcggttgaaa cgcgcaaacg ctgcaaaatc
     3361 gtcgccggtc agacagtgag ttttgcagga cacagcgtac aggttgttgc ctgatccctt
     3421 tcccggcctg tatcaacagg ccggttctct tttcagactt ttgccctcta cgccccgttc
     3481 atccatgctg atagcatcga cattttgccg aagcgaccat catgccaacc gttattacgc
     3541 acgccgccgt tcccctttgc attggtttag ggttgggttc gaaagtcatc cctccgcgtc
     3601 tgttatttgc cggaatcatc ctagcgatgc tgcccgatgc cgacgtattg tcgtttaaat
     3661 ttggcgttgc ttacggcaat gtttttggtc atcgcgggtt tacccattcg ctggtgtttg
     3721 cgtttgttgt cccgctctta tgtgtgctga ttggacgtcg atggttcagg gctgggctga
     3781 ttcgctgctg gctgttttta accgtctcgt tgctatcgca cagcttactg gattcggtaa
     3841 ccactggcgg taagggcgtt ggctggctgt ggccgtggtc agatgaacgc tttttcgctc
     3901 cctggcaggt gattaaagtc gcgccgtttg cattgtctcg ttacaccaca gcgtacgggc
     3961 atcaggtgat tatttccgaa ttgatgtggg tgtggttgcc ggggatgcta ctgatgggaa
     4021 tgttgtggtg gcgcagacga taaccggatg cgaaaactcg catccggcaa ttgcgcaatt
     4081 acttacgacg ccaggtggtc ccttgcgggc catcttccag cacgatcccc atctcgttaa
     4141 gacggtcacg tgccgcatct gccgccgccc agtctttcgc tttacgggca tccagacgct
     4201 gttgaattaa cgcttcaatc tcagccactt cgctgtcgtc tgcctgcgcg ccgctttgca
     4261 ggaacgcttc cggttcttgc tccagcaggc ccaatacagc ggaaagttta cgcaggtgag
     4321 atgccattgc attcgccgct gccatatctt ctgctttcag acggttcact tcacgcgcca
     4381 tatcaaacag cacggaatag gcttccgggg tgttgaaatc gtcgtccatc gcttcaataa
     4441 agcgcgcttc aaacgcttcg ccaccggcag gcgcaacggt tttatctgtg ccgcgcagcg
     4501 cagtgtagag gcgctccagc gccgcacgcg cctgcttcag gttctcttcg ctgtagttca
     4561 gctggctgcg atagtggccc gacatcagga agtaacgcac ggtttccgcg tcgtagtatt
     4621 tcagcacatc gcgcacggta aagaagttac ccagcgattt ggacatcttc tcgcggtcaa
     4681 ccatcaccat tccggagtgc atccagtagt tcacatactg accatcatgg gcacaggtgg
     4741 actgcgcgat ttcgttctcg tggtgcggga acatcaggtc tgaaccgccg ccgtggatat
     4801 caaagtggtt acccagctgc ttgcagttca tggccgaaca ttcaatgtgc cagccaggac
     4861 gacccgcgcc ccacggagac ggccagctcg gttcgccctc tttcgacatc ttccacagaa
     4921 cgaagtccat cgggttgcgt ttgtcatcaa ccacgtcaac gcgcgcgcca gcctgcagct
     4981 ggtcgagatc ctgacgcgac agcacgccat aagttggatc ggtcgggacg tcgaacatca
     5041 cgtcgccgtt gtccgccaca taagcgtgac ctttggcgat cagttgttca gtgagttcaa
     5101 taatttctgc gatatggtgc gtcgcgcgcg gctccatatc cgggcgcaga atgttcaaag
     5161 catcaaaatc tttgtgcatt tcggcaatca tgcgatccac cagcgccaca aagctttcgc
     5221 cattttcatt ggcgcgtttg atgattttgt cgtcgatatc ggtaatgttg cgcacatact
     5281 tcagtttata gccgaggaaa cgcagatagc gcgcaaccac gtcaaaagca acaaaggtac
     5341 gcccgtgacc gatatgacag agatcgtaaa cggtgattcc acacacgtac atgccgactt
     5401 ccccggcgtg aataggctta aattcctctt tttggcgtgt cagagtattg aagattttta
     5461 gcatcgaaga ttccgtttag acatgtgtgg gtaattgagt tgcgtataat acccatattt
     5521 cccgcgcgaa tcagcataca ttgcgtgatg atccgatcgt gcggttatgc tataacacca
     5581 ccctatatat gacccgaact gggttgaagc accaatcaaa cggaacagga tgcaaaaatg
     5641 gttactttcc acaccaatca cggcgatatt gtcatcaaaa cttttgacaa taaagcacct
     5701 gaaacagtta aaaacttcct ggactactgc cgcgaaggtt tttacaacaa caccattttc
     5761 caccgtgtta tcaacggctt tatgattcag ggcggcggtt ttgaacctgg catgaaacaa
     5821 aaagccacca aagaaccgat caaaaacgaa gccaacaacg gcctgaaaaa tacccgtggt
     5881 acgctggcaa tggcacgtac tcaggctccg cactctgcaa ctgcacagtt cttcatcaac
     5941 gtggttgata acgacttcct gaacttctct ggcgaaagcc tgcaaggttg gggctactgc
     6001 gtgtttgctg aagtggttga cggcatggac gtggtagaca aaatcaaagg tgttgcaacc
     6061 ggtcgtaacg gtatgcacca ggacgtgcca aaagaagacg ttatcattga aagcgtgacc
     6121 gttagcgagt aatcgtggcg acactcttta ttgcagatct tcatctctgc gtggaagaac
     6181 cggcgatcac cgccggtttt ctgcgttttt tagcggggga agcccgcaag gccgacgcgc
     6241 tgtatattct tggcgatctg tttgaagcat ggattggcga cgacgatccc aacccactcc
     6301 atcgccagat ggcggcagcg atcaaagcgg tgtccgattc cggcgttccc tgttatttca
     6361 ttcatggcaa ccgtgatttt ctgctcggca aacgctttgc ccgtgaaagc ggcatgacat
     6421 tattgccgga agaaaaggtg ctcgaacttt atggtcgccg ggtgttgatt atgcatggcg
     6481 acacgctgtg caccgatgac gcgggttatc aggcttttcg cgccaaagtc cacaaaccct
     6541 ggctgcaaat gctattcctc gccctgccgt tgtttgtgcg caaacgcatt gccgcgcgaa
     6601 tgcgcgcgaa cagcaaagaa gccaacagca gtaaatcgct ggcgatcatg gacgttaacc
     6661 aaaacgctgt ggtcagtgcg atggaaaaac atcaggtgca atggctaatc cacgggcata
     6721 cccatcgccc ggcggtgcat gaacttatcg ccaaccagca acctgctttt cgcgtggtac
     6781 tgggtgcctg gcatacggaa ggttcgatgg tgaaagtcac ggcggatgac gttgagctga
     6841 ttcattttcc gttttaaaaa acccacaact ttgctgattt cacagccacg caaccgtttt
     6901 ccttgctctc tttccgtgct attctctgtg ccctctaaag ccgagagttg tgcaccacag
     6961 gagttttaag acgcatgtct tcccgcaata atccggcgcg tgtcgccatc gtgatggggt
     7021 ccaaaagcga ctgggctacc atgcagttcg ccgtcgaaat cttcgaaatc ctgaatgtcc
     7081 cgcaccacgt tgaagtggtt tctgctcacc gcacccccga taaactgttc agcttcgccg
     7141 aaagcgccga agagaacggt tatcaggtga ttattgcggg cgcaggcggc gcagcgcacc
     7201 tgccaggcat gattgccgcc aaaacgctgg tgccggtgct gggcgtgcca gtacagagcg
     7261 ccgcactgag cggtgtcgat agcctctact ccatcgtaca aatgccgcgc ggcattccgg
     7321 tgggtacgct ggcgattggt aaagctggcg cggcaaacgc ggcattactg gcagcacaaa
     7381 ttctcgcgac tcacgataaa gagctacacc agcgtctgaa tggctggcgc aaagcccaga
     7441 ccgacgaagt gctggaaaac ccggacccgc gaggtgcggc atgaaacagg tttgcgtcct
     7501 cggtaacggg cagttaggcc gtatgctgcg tcaggcaggc gaaccgttag gcattgctgt
     7561 ctggccggtc gggctggacg ctgaaccggc ggcggtgcct tttcaacaaa gcgtgattac
     7621 cgctgagatc gaacgctggc cggaaaccgc attaacccgc gagctggcgc gtcatccggc
     7681 ctttgtgaac cgcgatgtgt tcccgattat tgctgaccgt ctgactcaga aacagctttt
     7741 cgataagctc cacctgccga ccgcgccgtg gcagttactt gccgatcgca gcgagtggcc
     7801 tgcggtgttt gatcgtttag gtgaactggc gattgttaag cgtcgcactg gtggctatga
     7861 cggtcgcggt caatggagtt tacgcgccaa tgaaaccgaa cagttaccgg cagagtgtta
     7921 cggtgaatgt attgtcgagc agggcattaa cttctctggt gaagtatcgc tggttggcgc
     7981 gcgcggcttt gatggcagca ccgtgtttta tccgctgacg cataacctgc atcaggacgg
     8041 tattttgcgc accagcgtcg cttttccgca ggccaacgca cagcagcagg cgcaagccga
     8101 agagatgctg tcggcgatta tgcaggagct gggctatgtg ggcgtgatgg cgatggagtg
     8161 ttttgtcacc ccgcaaggtc tgctgatcaa cgaactggct ccgcgtgtgc ataacagcgg
     8221 tcactggaca caaaacggtg ccagcatcag ccagtttgag ctgcatctgc gggcgattac
     8281 cgatctgccg ttaccgcaac cagtagtgaa taatccgtcg gtgatgatca atctgattgg
     8341 tagcgatgtg aattatgact ggctgaaatt gccgctggtg catctgcact ggtacgacaa
     8401 agaagtccgt ccggggcgta aagtggggca tctgaatttg accgacagcg acacatcgcg
     8461 tctgactgcg acgctggaag ccttaatccc gctgctgccg ccagagtatg ccagcggcgt
     8521 gatttgggcg cagagtaagt tcagttaagc aggtgctcat ttgattgccg gatgcggcgt
     8581 aaacgcctta tccggcttac cgctccggta cccattgtag gcctgataag acgcgtcaag
     8641 cgtcgcatca ggcatggtgc accgattgcc ggatgctctc atcactacag cgaaatacag
     8701 gtccccgctt cgcccgccag cgtctcttca attcgcgata acgccccaat ccacgcgggt
     8761 ttactacggc ttctgacata accactcacc gccgttacct tcggccccat cgaaccatcg
     8821 gctttggcaa atggcgctaa ctcatccggt gtggcatggc gaatggcacg ttgctgcggc
     8881 gttccccagt tttcatatac cgcatcagca tcggtgagga tcaccagtcc atcggcatta
     8941 atctgctcgg cgagcaacgc agcggcgaga tctttatcaa tcaccgcttc actccctgcc
     9001 ccatcttccg tcacaggcac accgccgccg ccactgcaaa tcaccacatg cccctctttg
     9061 agcaacaact cgatggcttc gctgtcgaga attttacgcg gttgcggaga cgccaccacc
     9121 cagcgcaaat atttaccatc acgtttcatc tgccagccgt aagccgcttc cagtgcctct
     9181 tgttcttctg gctgataaac cggaccaata aatttctctg gctgcaaaaa cgccggatca
     9241 tcaggcgaaa cctcaatgcg cgtcagcacc gtcgtaacag gcggcatctg cggctgtgcg
     9301 ctcaagctct gcgccagcat atagccaatc atcccctggc tttccgccac cagcacatcc
     9361 agcggatacg gttctacctc tttccacgcc agattctgta acgccagcag ccccacctgc
     9421 ggcccgttgc cgtgaacaat cgccagccga taagaacggg ccaggcgtgc cagcgcgggt
     9481 acagcactgg cgatattgcg atattgattt tccgccgtca gcgcctcacc gcgctggagt
     9541 aaggcgttgc ccccaagagc tacaaccagt gttttcatgg ttttccttgt aataattgtt
     9601 ggccaagcca gaaccccagc agcgtatctg cccctgacgt atgccccagc gccagcagcg
     9661 aatcaatcgc aacagcggta cgtttcgggc aactcagagc atggacaaag tgcaggagtg
     9721 gcgaggcgaa atatccttgc gcggcataac gtaaataact gacgctcacc gctgtggtaa
     9781 cgagttgaag attgtcggaa caggcaaaaa acgggcgacc ggcgcgcgca tctaaagcgc
     9841 cataatacca ggccgccagc agcattccgc tcaacgtgtc atcatgactt ggcgttaatc
     9901 cggggccttt acccagccag tgccgccagt cggtcttaac gccattgagc gcggcctgaa
     9961 aacagtgacg aaactggcgt aattcagcgg gcagcggatc gcttgccgcc atcgccagtg
    10021 gcccgaaaag tccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat
    10081 gcgaaggcgg cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc
    10141 cgaggcgaat ccctttagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata
    10201 acgcatcgaa ttgcgtgcgg cgaaattaca cattcccgtc aagctataga agaaacgctg
    10261 ccctggcttt cctcttttgg cgctgaccca acgggtggga tgacccgttt actttattcg
    10321 ccggaatggc tggaaaccca gcagcaattt aaaaaaagaa tggcagcaag tgggctggaa
    10381 acacgtttcg atgaagtggg gaatttatac ggtcgcctga gtggcaccga atatccacag
    10441 gaagtggttc tgagcggttc gcatatcgat accgtggtta acggcggtaa cctcgacggg
    10501 caattcggcg cgctggcggc gtggctggca attgactggt tgaaaacgca atacggcgca
    10561 ccgttacgta cggtcgaagt ggtggcgatg gcagaagaag aaggcagccg cttcccgtat
    10621 gtcttctggg gcagtaaaaa tatctttggg ctggcgaatc ctgacgacgt gcggaatatc
    10681 tgtgatgcca aaggaaatag ttttgccgat gcgatgaagg cttgcggatt tactctgcct
    10741 aacgccccac taactccgcg tcaggatata aaagcctttg ttgaactgca tattgaacag
    10801 ggctgtgtgc tggaaagtaa tgggcaatca attggcgtgg tgaatgcaat tgtcgggcag
    10861 cgtcgctata cggtgacgct gaacggcgag tcaaaccatg caggcaccac gccgatgggt
    10921 tatcgtcgtg atacggttta cgctttcagt cgcatttgcc atcagtcgat cgaaaaagcg
    10981 aaaaagatgg gcgatccgct ggttctgacc tttggcaaag tagagccgcg cccgaatacg
    11041 gtaaatgtgg tgccgggtaa aaccacgttc accattgatt gtcgtcatac cgacgctgcc
    11101 gtgctgcgcg atttcaccca acagttagaa aacgacatgc gggcgatttg cgatgaaatg
    11161 gacattggta ttgatatcga tttatggatg gacgaagaac ccgtgccgat gaataaggag
    11221 ctggtcgcca ccctgacaga attgtgtgaa agagaaaaac tgaattaccg ggtgatgcac
    11281 agtggtgccg ggcacgacgc gcaaattttc gcgcctcgcg taccaacctg catgattttt
    11341 atccccagca tcaacgggat cagccataac ccggcggaac gcaccaatat tactgacctt
    11401 gccgaagggg tcaaaacgtt ggcactcatg ctttatcaac ttgcctggca gaaataagga
    11461 gtcataaatg ggatatttaa ataacgtcac cggttaccgc gaagatttac tggctaaccg
    11521 tgcgattgtt aaacacggta atttcgcact gttaaccccg gatggtctgg taaaaaatat
    11581 tattccgggc tttgaaaatt gtgacgcgac aatcctctcc acgccaaagc tgggtgcctc
    11641 ttttgttgat tatctggtca cactgcatca aaacggtggc aaccaacagg gcttcggtgg
    11701 cgaaggcatt gaaacgttcc tgtatgtgat ctctggaaat atcactgcca aagccgaagg
    11761 caaaacattt gccttaagcg aaggtggcta tctttattgc ccaccaggct ccttaatgac
    11821 gtttgttaac gcccaggccg aagacagcca aatcttttta tataagcgcc gctatgttcc
    11881 ggtagaaggc catgcaccgt ggctggtttc tggcaatgcc agcgaactgg aacgcattca
    11941 ctatgaaggt atggatgatg tgatcctgct ggattttctg cccaaagagt taggttttga
    12001 tatgaacatg catatcctct cttttgcacc aggtgccagc cacggttata tcgaaacaca
    12061 cgttcaggaa catggtgcct atattctttc cggtcagggg gtttataacc tcgacaataa
    12121 ctggatcccg gtgaaaaaag gcgattacat ctttatgggc gcttattctt tacaggctgg
    12181 ttatggcgta gggcgtggtg aagcgttcag ctatatttac tcgaaagatt gcaaccgcga
    12241 cgtagagatt taagtcatac cactcgcctg attattttta gctatgttgg attttgccac
    12301 ggtttatacc gtggcttttt ttgtaacatt gcctgatgcg cttcgcttat caggcctaca
    12361 tgacctctgc aatatataga atttgcgcga ttttcaggaa ggatatagta ctcatgccgc
    12421 atccggcatg agtactgcgc tcttatacga aataaatcgc atccgcttta aaggcaagag
    12481 tcagttttta attccctgac ctattttaat ggcgcaggca atatttcgcg cgctgttgaa
    12541 gagattggtt tcaccgctgg cgagcacttc ggctaaaggt gccagacgag gcaaaatgct
    12601 gaatagcgcg tcaatgccgt actggtgcac cacttccacg ccatcaccca atacaccagc
    12661 aatcccaatc accggtacat taaactgctt cgccaccgac gcaacgccca gcggcgcttt
    12721 accgcctgcc gtttgcgagt caatgcgccc ttccccggtg atcaccagtg ctgccccctg
    12781 caccgcctgc gcaagattga ccgcattcaa cacaatctca atgcccggtt taatatccgc
    12841 attgagaaat accgccgccg caatccccat accgcccgca gcgccgccgc ccgccatctg
    12901 acagacatta atttcagttt gctgttgtaa aacacgggcg taattttgca gcccctgttc
    12961 cagctcttca accatctccg gcgttgcccc tttttgcggg ccaaataccg ccgccgcgcc
    13021 gcgtgcccct accagcgggt tatcaacgtc acatgccact tcaatatggc tgttcgccag
    13081 acgcggatcg cattcctcca tctcaatgct tgccacgcgc gctaaattgc cgccgttagc
    13141 cgccagcacc tggccgtcgg catcaaggaa acgcacacca agcgcctgcg ccatgcccat
    13201 accgccgtcg accgtcgcac tgccgccaat gccgagaata atatggcgaa tgccgttatc
    13261 cagcgcatgg cgaattaact cccccgtacc aaaactggag gccagcaacg gattacgctt
    13321 ttcaggcgcg accagcatca ggccacttgc tgccgccatc tcaatcaccg ctgttttccc
    13381 gtctccggta aggccataaa aagcattcac tttttcgccc atcggcccgc agacttcaag
    13441 cgtcacgatg ttgccacccg tcgcggcgac catcgcatcc accgtccctt cgccgccatc
    13501 cgctatcggc aaacagatat agtgcgcatc gggaaagagg gtcgaaaacc cggctttaat
    13561 tgcctgacaa catttttctg cacttaagct ctccttaaaa gagtctggcg caatgacaat
    13621 cttcattatc aatccttttt caacaaatta ttcga
//
LOCUS       DAJTXY010000108        13540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTXY010000108 DAJTXY010000000
VERSION     DAJTXY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.108"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(1..898)
                     /locus_tag="OO566_003184"
                     /pseudo
     CDS             complement(1..898)
                     /locus_tag="OO566_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1139..1939
                     /locus_tag="OO566_003185"
     CDS             1139..1939
                     /locus_tag="OO566_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6606371.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2017..2787
                     /locus_tag="OO566_003186"
     CDS             2017..2787
                     /locus_tag="OO566_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6606372.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2835..4193)
                     /gene="mltD"
                     /locus_tag="OO566_003187"
     CDS             complement(2835..4193)
                     /gene="mltD"
                     /locus_tag="OO566_003187"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6606373.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4265..5020)
                     /gene="gloB"
                     /locus_tag="OO566_003188"
     CDS             complement(4265..5020)
                     /gene="gloB"
                     /locus_tag="OO566_003188"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6606374.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5054..5776
                     /locus_tag="OO566_003189"
     CDS             5054..5776
                     /locus_tag="OO566_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6606375.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5773..6240)
                     /gene="rnhA"
                     /locus_tag="OO566_003190"
     CDS             complement(5773..6240)
                     /gene="rnhA"
                     /locus_tag="OO566_003190"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6606376.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6305..7036
                     /gene="dnaQ"
                     /locus_tag="OO566_003191"
     CDS             6305..7036
                     /gene="dnaQ"
                     /locus_tag="OO566_003191"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6606377.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7169..7245
                     /locus_tag="OO566_003192"
     tRNA            7169..7245
                     /locus_tag="OO566_003192"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7203..7205,aa:Asp,seq:gtc)
     gene            complement(7376..8422)
                     /locus_tag="OO566_003193"
     CDS             complement(7376..8422)
                     /locus_tag="OO566_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6606378.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8433..9428)
                     /gene="tssG"
                     /locus_tag="OO566_003194"
                     /pseudo
     CDS             complement(8433..9428)
                     /gene="tssG"
                     /locus_tag="OO566_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9425..11308)
                     /gene="tssF"
                     /locus_tag="OO566_003195"
     CDS             complement(9425..11308)
                     /gene="tssF"
                     /locus_tag="OO566_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6606379.1"
                     /translation="MDPRLLGYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRPLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11324..11818)
                     /gene="tssE"
                     /locus_tag="OO566_003196"
     CDS             complement(11324..11818)
                     /gene="tssE"
                     /locus_tag="OO566_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6606380.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11815..12645)
                     /locus_tag="OO566_003197"
     CDS             complement(11815..12645)
                     /locus_tag="OO566_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HCR6606381.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12632..13534)
                     /gene="tagK"
                     /locus_tag="OO566_003198"
                     /pseudo
     CDS             complement(12632..13534)
                     /gene="tagK"
                     /locus_tag="OO566_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596542.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3101 a         3524 c         3616 g         3299 t
ORIGIN      
        1 cctccgggag ctgtttttac atgctcgcta aggaaatcga taaaagcccg gatgcgcgta
       61 cttaccgcac ggtcgctgta atagacggca ctgaatggca tttccactgg caacacttta
      121 tctgccatta actccaccaa ttatccgcga gcgatttctt tgtcgatcat gtagtcggac
      181 aaacacgcaa tcccgttgcc actcaggcaa agctgtttca gtgtttcccc actattggat
      241 gacaaaccgt acttcacctc atgtaattgt ccatcactac aggctatcgg ccaggtattg
      301 agggaagcgg gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc gatcgtttct
      361 ggcttcccgt agcgggaaat ataatcgggg gaggcgataa tttttcgata actgttaaat
      421 aacggcctgg cacgtaagct ggagtccgtt aacgtaccag cgcgtatcgc gacatccact
      481 tttctttcga tcaaattaat aatcgtttcg gaggagacta gcgataaagt gacttccgga
      541 tagcgttcac ggaaaggctt aattaacggc atcagaaagt gcagcaccac tggagttgcg
      601 gcatcgatcc gtaacagtcc acgcggtgta ttacgcgtct ccataatttc tgattctgcc
      661 gctgccatct cctgcaaaat tgactgtacg cgacgaaaat aacgctcgcc ttcttccgtc
      721 aggctaagtt gccgcgtggt ccgattaagc aggctaacgc caagtttcat ttccagcttt
      781 ttcaccgccc ggcttaccgc tgagtttgct tgccctaaaa ttgttccgct gcccggctaa
      841 agctgccgct ttcgacgacc gaaacaaaaa tggcgagttc ttccgacgtg gctttcattt
      901 ttgctcctgt tgcaaaatag atgagatatt ttgaatttat ttgtcattaa accatcagga
      961 tgcgtgatat gtcatccgat ttaatgttct caataatgag caaatttctg accggtgtaa
     1021 gcacttgctt acataacaat atacaattgc tcgttgaaag agtgagctaa aatccctata
     1081 acagtagaac cctcccgagt gcggaagggt tgacgtaata gaggtttcaa agtcaaaagt
     1141 gcgaaaaaac acctatgcca tgcgctatgt tgccggacaa cctgcggaaa ggatcttacc
     1201 gccggggtct tttgcgagca tcggccaggc attaccacct ggagaaccgt taagtaccga
     1261 agagcgtatt cggatcctgg tgtggaacat atacaaacag caacgcgctg aatggttgtc
     1321 ggtattaaag aactacggca aagatgcaca tctggtgtta ttgcaggaag cgcagacaac
     1381 gccagagtta gtacagtttg cgaccgctaa ctatcttgcc gccgatcagg tgcccgcttt
     1441 cgtgctgcca caacatcctt ccggtgtaat gaccctttcg gcggcgcatc cagtgtattg
     1501 ctgcccgtta cgtgaacgag aacccatttt gcgtctggcg aagtcggcac tggtgacggt
     1561 ctatccatta cctgacaccc gcctgttgat ggtggttaat atacacgccg tcaacttcag
     1621 tctgggcgtg gatgtctata gtaagcagtt acttcctatt ggtgatcaga tagctcacca
     1681 cagcggcccg gtcattatgg cgggagattt caatgcctgg agccgtagaa ggatgaacgc
     1741 gttatatcgc tttgcgcggg aaatgtcgct gcgccaggtg cgttttaccg atgatcagcg
     1801 ccgtcgggcg tttggccgcc cgctcgattt tgttttctac cgtggtctga acgtcagcga
     1861 agcttctgta ttggttacgc gcgcttccga tcacaatccg ctactcgttg aattcagtcc
     1921 cggcaagcct gataaataag gtatgtcagg tctgccacag ggcagaccaa cgtttggcgc
     1981 tgtcctttta tactcaaaca acaaaaggac agcgcaatga caacacaatc ccaccatgac
     2041 cacgtagaaa agcagtttag ctctcaagcc tgcgaatatc tgaccagtac cgtacatgca
     2101 tccggacgag atttacagcg cctggcggtg cgtctggctg attatcctgg tgcaagtgta
     2161 cttgatatgg gctgcggagc agggcatgcc agctttgttg ctgcgcaaaa cgtgagcgcg
     2221 gtggtggcgt atgacttatc tgcccaaatg ctggatgtcg tggcacaagc tgccgaagcc
     2281 cggcaactga aaaatatcac cacccgccag ggatatgctg aaagtctgcc atttgccgat
     2341 aacgcatttg atattgttat cagccgttat tctgcccatc actggcatga tgttggtgca
     2401 gcactgcgtg aagtgaaccg gatattgaaa cctggcggta ggatgattgt gatggacgta
     2461 atgtctccgg gtcatccagt gcgcgacatc tggttacaga cggtagaagc attacgcgat
     2521 acctctcacg tacgaaacta cgccagcggt gagtggttga ggttaatcaa tgaagccaat
     2581 ctgatagttg ataatttaat tacagataag ttaccgctgg aattttgttc atgggtcgcg
     2641 agaatgcgta cgccagaagc gttagtagac gctattcgca tttaccaaca gagcgcatcg
     2701 acagaggtga gaacgtattt tgccttgcag aatgacggct ctttcaccag tgatatcatc
     2761 atggtagaag cacataaagc ggcataaata aaaaaggcac cgggggaatc ggtacctttt
     2821 tattatctgg tttgtcagga atctggcatg ttgttgtttt tcacaaacaa cgtcagcttg
     2881 tcgcctggtt gcagattcgc agtgtcgctg ttccagcgca tcacatcttt gatgttcacg
     2941 ccgtggcgtt tagcaatgct tgaaagcgaa tcgcctttgc gcacacgata cgtaatgcta
     3001 tcgctgttgt ttgccagtcg ctgtgcgcta ctgcctgcac caatcgtcaa actttggcct
     3061 ggcttcagct tagatccgcg cagtttgttc cactgctgca aatctttggt gcttacgccg
     3121 agacgtgaag cgatgcttga aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta
     3181 agcggcgtat tgtcggcaac cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga
     3241 gattcacgca gttgatctgc atgcttcttt ggcaccatca cgtactgcgg cccacttgcg
     3301 cccagcgtgg agcctttcac gccagcgttg aatgtcttca gcttgctgac ggaaatcccc
     3361 gccatatctg caaccttcgc catttcaacc gggctactca ggtgcacacg cgccagagca
     3421 cggctttcat cggtcgttgg cagacgtacg ccataacgct tgctgttttt gagaatatca
     3481 ctcaatgcca gcattttagg cacgtactgc ttcgtttcct gcggcaacgg taacgaccag
     3541 aagtccgtgg atttcccacg cgctttgttc gttttaattg ccttcatgac ccgaccttcg
     3601 ccgctgttat aagccgctac ggtcagaagc cagtcgccgt caaacatttt gttcagacgc
     3661 tgcatcatat tcagcgcggc agttgttgaa gcaacaacat cgcgacgcgc gtcataattg
     3721 cgggtctgtt tcaaaccata attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg
     3781 gcattggcgc cagacgttgc gtgaggatca aaagcgctct ccactatggg tagtagtacc
     3841 agttccatag gcatgttacg ttttttaact tgccctgcta tccagtacat atacggctct
     3901 gcccgtaaag ttacatcgtg gagatagctc ttattgcgta aatatttctg tttctgttcg
     3961 cgaatccggt cattttccgg aattcccatc tttagctcgt cgccaatgaa agcccacaag
     4021 tcaccatctg gcgcgataga cgtcccatcg tccatccatc gtgcctgact tgtaaacttt
     4081 gctgcttccc cttgaccagc tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta
     4141 ctctggcaac ccacgagcag gacagaggcg agtaatatcg cttttgcctt catgtgtgtg
     4201 tcaatagttg cttaaaagac gaccgatcat aacggcgaac ggagccgatg acaagaaagt
     4261 tttatcagaa cctatctttc tttgacctta accatgcaaa acgctcttca ggttgttgca
     4321 ataatgtttc ttcattaatt acattaatta aatcaatatc ttccgttctt aaaaaaacat
     4381 taatttgccg ctcatttttc agaattacgg gtagtgttat ttgatttttt gcccgtaact
     4441 ccttaacttt acgataataa tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa
     4501 acttcatatt tgataacgta tattcatgag cacaacatac caatgtatcg tcaggtaacg
     4561 cacttagctt tttaagtgat tgatacattt gtaaagctgt cccttcaaac aaccgaccac
     4621 acccaccaga aaacagcgtg tcgccgcaaa atagataagg tttactgaag taacagatat
     4681 gtcctaaagt gtgacccggt gtggtaatta cactaaattc atgccctaaa acgaaggcag
     4741 tttcgccatc tttgactacc tgtgttgttc ccttatcttg tgtctcttgt ggaccataca
     4801 ccacaatttg cggaaacttt tccaccagtt cttttacgcc gccaacgtga tcgtggtgat
     4861 ggtgggttag aaatatggcc tccggttgcc agttattggc ggtaatggcg tttaatactg
     4921 gctctgcgtc tccgggatcg acaatcaggc agcgacctgc ctcatcattc aaaacccaga
     4981 tgtaattgtc atcaaaggcg ggaatactgt taagattcat agattacctc tcagtgtaaa
     5041 acggaaggtt gtgatgaaac cggcaagagt ccctcaaact gtcgtggctc ctgattgctg
     5101 gggcgatttg ccctggggag agctttatcg caaggcgctg gagcgccagc tcaacccgtg
     5161 gttcactaaa atgtatggtt ttcatctgct taagattggc aatttaagcg cagaaatcaa
     5221 ttgcgaagcg tgcgcggttt ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca
     5281 ggtgcaggcg gatccacttc atcttccttt tgccgataaa tccgttgata tttgtctact
     5341 ggcacatacc ttgccgtggt gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt
     5401 actgattgat gatggctggc tggtgattag tggcttcaac cccatcagtt taatgggatt
     5461 acgcaaactt gtgccggtat tgcgcaaaac ctcgccctat aatagccgga tgtttactct
     5521 gatgcggcaa ctggactggc tctctttgtt gaattttgaa gtgctgcacg ccagccgttt
     5581 ccacgttctc ccgtggaaca aacacggagg aaaactattg aatgcgcata ttcctgcgct
     5641 tggttgctta caacttattg ttgcccggaa acggactatt cctttaacgc taaatccgat
     5701 gaaacagagt aaaaacaagc cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg
     5761 taaaccacag gcttaaactt cgacttggta gcccgtatct tccagtgtgg gattcatcgc
     5821 cgcggcacga gccagttcat cacagcgttc gttttccggg tgtccggcat ggcctttaac
     5881 ccattcccat ttgatttgat gctgccccaa tgcagcatca agacgttgcc agagatcgac
     5941 attttttact ggttttttgt ctgctgtttt ccagccacgt tttttccagt tatggatcca
     6001 ctgggtgata ccctggcgga catactggct gtcggtactc aaaatgactt cgcaatgttc
     6061 ttttaacgcc tccagcgcga caatagcggc catcaactcc atacggttgt tggtggtgcg
     6121 ggtgtagcct gcgctaaagg ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc
     6181 cccaggtcca ggattaccca gacacgaacc atcggtgaaa atttctacct gtttaagcat
     6241 ctctggtaga cttcctgtaa ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac
     6301 cgctatgagc actgcaatta cacgccagat cgttctcgat accgaaacca ccggtatgaa
     6361 ccagattggt gcacactatg aaggccacaa gatcattgag attggtgctg ttgaagtagt
     6421 gaaccgtcgc ctgacgggca ataacttcca tgtttatctc aaacccgatc ggctggtgga
     6481 tccggaagcc tttggcgtac atggtattgc tgatgaattt ttgctcgata agcccacgtt
     6541 tgccgaagta gccgatgagt ttatggacta tatccgcggc gcggagttgg tgatccataa
     6601 cgcagcgttc gatataggct ttatggacta cgagttttcg ttgcttaagc gcgatattcc
     6661 gaagaccaat accttctgta aggtcaccga tagccttgcg gtggcgagga aaatgtttcc
     6721 cggtaaacgc aacagcctcg atgcgctatg tgcccgctac gaaatagata acagtaaacg
     6781 aacgctgcac ggggcattac tcgatgccca gatccttgcg gaagtttatc tggcgatgac
     6841 cggtggtcaa acgtcgatgg cttttgcgat ggaaggagag acacagcagc aacaaggtga
     6901 agcaacaatt cagcgcattg tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga
     6961 tgaagagctg gcagcacatg aagcccgtct cgatctggtg cagaagaaag gcggaagctg
     7021 cctctggcgg gcataaatac atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg
     7081 cagcaaacga ttcaaaagat gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat
     7141 tcgcctcgtt cccaacggaa cacaacgcgg agcggtagtt cagtcggtta gaatacctgc
     7201 ctgtcacgca gggggtcgcg ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc
     7261 gaatcagtaa tgattcgggc tttcgtcgtt ttggtgaagt gagaattttt cactccgaag
     7321 taaaacggat atgatttaaa aaatggccag acattataaa ttctggctac ttctgttact
     7381 ccagcgcatc ccatttctcc agcagttcta ctggaatttc atttattacc tccgagacgc
     7441 gttttccgac caggcgttct gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc
     7501 tgtgctcaaa ccagcgacgg atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga
     7561 tctgccgggg ttctgcgccg gtcatcggtg atatttcctc cgcgatatgc accggtagcg
     7621 caatctgctc tgcctgcatc tgttcagtat gaacgatggt ggcgttttcc ggctgctcag
     7681 cctgtggttg tggcaggatc tccggcgtgg tgctctgcac tgctcccggt aacagggcca
     7741 gcagttgcgt cagacgggag aagtccggtg cctggtcatt cagcgtttca cgcgcatggc
     7801 gttgcagccg ctcagtggtc tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc
     7861 gcgcttccag gtctgccagt tgctggcgta ccgattctgg tgccagcgca tccgccgggc
     7921 gtaatgcaga tagcgaacgc tccacatccc gcacctgaag gcgcatggca gcattatttg
     7981 acagtgtaat gccacgaata tccgccatta caccttcgtg gtccagcaac gccgccagcg
     8041 cgttactgcg tgccaccgca gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag
     8101 gatggatggc gtccgggtac gtggagcaca acgtttccag cagcgtgagc gcctccgaca
     8161 gtcccctggc accggcctgc tggatacggc agcgcagcag gacaaccaga atgcggatat
     8221 ctttgctgcg catcagcaga cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt
     8281 ccggcgtact gacaaaatcg ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca
     8341 gcagcaggta ttccggatcg tactccagat ccgggccgca ggggcattcc gccgaaattg
     8401 ggtcagacag gaatatttcc atttaattat cctcagtgcg ccaggtagtg ttctggttcg
     8461 aaaatcatgc ccgtcaccgg gttgtcgtgc atggctttac ccgcccatgc agtccaaccc
     8521 agttggtacg gaccaccggt aacagccgga ggggcggcgt ggggctttag ctgtagctca
     8581 atttcccagc agtattcata accactaaag gcgcggacca gttctgtcag gacgggcagg
     8641 ttattgctgc caggcagaaa acgtcggtaa tcctccagcg tcagcgggcc gataaccagc
     8701 cggaacttat actgcatgtc cggcacggcc tggccgatga gtgcactatt gccaataacg
     8761 gaagaggggg caggcatccc cagccgggtg atctcatcgt ccgccacggc tatccagtgc
     8821 aggacatact cctgtatccg gaaaggtaca ccgaaatagt gcgccagcgt ggtggccaga
     8881 ccgtccggat tgcgggactc acgcaccaga tgggccgatg ccgccagacg agcgtgatct
     8941 ggcaacgggc tgtcagcgct ctcccgcaaa tcctgcccgc tgaggctggc gacataaaag
     9001 gcaaagcggt catgttgcgg tttatccaga ccgcccccag cggactgggc gctgcgccat
     9061 gcctggtaaa actgcgtcag ccagcggtga tgaaaaagat cagaaaaacg ggtcagcgtc
     9121 ggatcgcgtc ggctttcagt ccggttacgc gccagttcgg tgtagtgcag cggcagtgga
     9181 ccgttcggcc cccacagccc gaggctgtac agactgatgt gcaggcgccc ttcctgccag
     9241 cccacactgg cgatttctcg tggcgcaaag gtcagcgcgg gcgtttgtcc gaggcgaaat
     9301 ttttccatcc gtggttgcta ggtgttgccc gccggaacgg tacacaactg cacatccacg
     9361 cggcgcatca ggttcagaaa accgtaatgc caggggggtt cctgcgcctg agtgaaatcg
     9421 ttgctcatac gcttccccgc tgtcccgccc tgacaggcca ggtcataatt ttgccgcgct
     9481 gcatgctgtg cagcgtcatc tgcgagaagg tattaatgga aacatggcgg gcgatatagt
     9541 gttccagcac caggccgaac agatacggac tgatgccgga aaaaccttct tcatccaccg
     9601 tcagttcaca actgacgcca cgcccataga ccagtagtcc ggaaccgggc agacggcggg
     9661 tgaccggggt cgttttacaa ccaatcaggc tgcgcacctg gcgcgattgc ggactgtcat
     9721 gagcaggaat aaacaggttc agcagatctc gcaacgcctg gccgccggta cggtgatcca
     9781 gatcggccag cggcagataa ttaaaagata actggcggat caaccgccat gccatctcac
     9841 gttcagccag cgggggctgc ggcgagcgtg gcgggcggat aagccctacg cccgccaccg
     9901 ggatggctgc atccacggtt agatcgtccc ggccattgcg ggcaataaga cagggcagat
     9961 cgcggttagt gaccatggcc gtgatggtga tgtggcgcag attttccggg tacggcgctt
    10021 catactggtc aaccagcgac aggaacactt ccgagcccgt atagggggta cgggtgccgt
    10081 aacggcgggc gttttctgat aagcggcggg gctcccggcg cagggaaaaa taacgtccgt
    10141 gattgccttc atcattattg cgggtgtggt agagcgggcg aaaggccatc ttccgtgtgg
    10201 tgtcggtttc cagcccttcc acttcctgga ccgagaacac ctcataatcc agcggatggg
    10261 ttctgtccac caccagatgc tgttccgtcg tgctgtgcgt gacatcgatc cgggcggtga
    10321 cgcggggaaa caggttgata accggggtac agaacaggct gaactgtgcc gcatttgtct
    10381 ggtggatcag ccagtccggc ggcaggcggt tcagcaggat aacaatctcc gccacgccac
    10441 cgtcaatttt ctgtaatccg gcagacaggc ctgtcggggt gaaaaaatag aagcgttccg
    10501 gacaggcaaa atattcatgc agcaggttat gaccgtgaaa cacgttccac gccagcggca
    10561 gcagcccctg atccggctcc aggccttcgt acataacggg ctgttgcaga ttcacatcca
    10621 gcgcgccgtc aaaatgcccc ggaatgccag ccagggtcgc gatggcgctg gtatgtagaa
    10681 gttctaacag gtgtgaggcc gtacgctcct cgccgcagag ataaaacggc agcctgtcca
    10741 gtccggcaag ctgactgaac gttagttcac caaaggtgcg cagggtaata cgcagcgccc
    10801 cggcgacgtg aatgttggcc ggcaggtagc ggtgcagggc aggcatatcc ggtggcgcgg
    10861 cggtcagtcg cgcctcttcg atggcgagcg gccacagcgt tacatcctgg ctgctgcgaa
    10921 actggcaggc agtgctttcg ccttccggaa tggcggagaa aaaggctgtg tcgcgcggga
    10981 cggtaaatcc tttcgccaga tcgccttctt ccgtatcggg atgcaactgc gccacgctca
    11041 ttgagggcgt gggtgtgacg taattggggc tgaccacttc cagcgggcgc tgggtgaaac
    11101 gggggaattc agcgtcaatt tttagctgcg tgcgggcggt caggaagctg aaggcctcga
    11161 ccattcgctc tacatacgga tcggcaatat ccgttccctg catgcccagc cgggcggcga
    11221 ctttgggatg acgggcggca aattcggctc ccgtttcccg cagatagctc agttcgcggt
    11281 tgtagtatcc cagtagccgt ggatccatcg gtctctctct gaattaaaaa aacgtaatgc
    11341 gactctgctc catatccagc gcgctacgta cccggaatgc agtcggatac ggctgcgtga
    11401 ggatctcgcc gcgaatttca aactgtaatg tgttatggcc actctggcgg cgctcatcct
    11461 gaagtgggat gatttgcagg gtggacgctt tcagccgggg ttcaaaccgg gtgatggttc
    11521 gcaaaatggc cctgcggata tcgtcccact tgtgctcata cagaaagctg cccgccagtg
    11581 gcggcaggcc gtagttaagt acggacgctg ccgcgtacgg ataacgtgca gcatcaatat
    11641 catcttcatg gctgacggtg ttgagcagaa atgacaaatc ccggcggatg atctccttaa
    11701 tctgaaccgg gctgacgctg ataccgtggt cgcgtttctg gtttggggcg tcgtcacaca
    11761 ggcggtcaaa cagcgtgggc agcaggtgat tgcctggacg ctggcgggaa gcgctcacgc
    11821 gtcgcttcct tcagcggtgt taaaggtaca ggtggacaaa tccagcaggc tgatatcgcc
    11881 gtgaccggtg agccacaccc tctgacccag cgcctgtacc agtgtctctc ccggaccgtc
    11941 ctgccagacc gtttcccggc acagacgcag ggagtcggca gccttttcag agccgctgta
    12001 gcggggaaac agccaggcac tgtgtgtatc gccattgttc agggtgacgt tgacgggttt
    12061 ccagatcaaa tcgatcagat gcgcaggccg cggcgatttc agcgaacaga tttgtgaaaa
    12121 cggcagccag atatatgctc cgcccgcgat cagttcgagt accggaccgg tacgggagtc
    12181 gctgtcgcta atccagtcaa atgcgccgcc attccactgc ccggaagtgt ctgttatggc
    12241 ctcaagcgcg cggctgcggt gagcatcagc ttcctgcgct tcaccacggg cgttacaggc
    12301 gagggtggtc agcagatcct ccatccaggc gggcggcggg agaataacac cgggccgctg
    12361 ttcgccctga aagcaggcgt ggcggtatat ctcacagcgg atcagctcgc caaatacctg
    12421 cgcttcgcga gtataatttg cgtccatccg ggcgcatagc tggatctgtt gcagtgcccg
    12481 cgaccagtcg cctgccacgc acaataactg aaacaggctg tggcggcggg aggcctgcgc
    12541 tgggtgcatc ctgacctgtt gctctgccat ctgaatgccc tccaccacgg aataatcccg
    12601 cagcagcgcc gacaaggtaa tcggtagctc gttattgttt gtcatgatat atctctccat
    12661 gttgtgccgg gtttatccgg taatggttgt caatgccaat gatgcgatgc tctttccgtg
    12721 tcagatctgg cagtacggtg ttatgcgctg tttccggggc gtattccggg gaaagtaaat
    12781 gcaggatatc agggggagtc ttttcgacag gccagatagt ttcatcttcg ccagtggcat
    12841 ccagtacatt gagaatagcg tcaatgctta acccaccagt gaccatatcc tgcaatgtct
    12901 cggcttcggt gaccaggtca cgcaatgaat ccagatcctg cgaggagggc tcttcgttaa
    12961 aaggaaaagg atcagcatgg cgattaagat cttgttcggg agataacaac gcctcgcggt
    13021 attcctgata aagtcgggtc agggcatttc ctttttccgc accttctccg gtggaggagg
    13081 ctccatgaac aggaatgata tcgaatggat tttgtgatgt cagatgctgc tgcctgaacc
    13141 aatccagatc cagatattcg gttattgctt ccgacgtgat ggcagtctgc gatatcgttt
    13201 ttactgtgct caggtccgga gatgatggat tgccatgtac aaggcactat gaagataacc
    13261 cccattctat caggtcgcct tcattcagac gcatacggtg atgaggttcc acaatctggt
    13321 cgtttaccgt acagcaatat tcggtagact gattgataat ccaccatgct tcttcgtggc
    13381 gggtcagagt gagggcaatc tttacatcat cattcacctt aaaaacagga atccagggag
    13441 aggtaacggt gaagataatt tgtgttcctt cagtcgcgta aattgtgtta tcggcgatcc
    13501 cgtgcgcctg tattttttgg agtccccacg taatatttgg
//
LOCUS       DAJTXY010000109        13439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTXY010000109 DAJTXY010000000
VERSION     DAJTXY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.109"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..353
                     /locus_tag="OO566_003199"
     CDS             <1..353
                     /locus_tag="OO566_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606382.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
     gene            510..1259
                     /locus_tag="OO566_003200"
     CDS             510..1259
                     /locus_tag="OO566_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6606383.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1281..1847
                     /gene="yieF"
                     /locus_tag="OO566_003201"
                     /gene_synonym="chrR"
     CDS             1281..1847
                     /gene="yieF"
                     /locus_tag="OO566_003201"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6606384.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1901..3238)
                     /gene="adeP"
                     /locus_tag="OO566_003202"
     CDS             complement(1901..3238)
                     /gene="adeP"
                     /locus_tag="OO566_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6606385.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3403..4068
                     /gene="yieH"
                     /locus_tag="OO566_003203"
     CDS             3403..4068
                     /gene="yieH"
                     /locus_tag="OO566_003203"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6606386.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4135..4608
                     /locus_tag="OO566_003204"
     CDS             4135..4608
                     /locus_tag="OO566_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR6606387.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4657..5244
                     /locus_tag="OO566_003205"
     CDS             4657..5244
                     /locus_tag="OO566_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR6606388.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5306..6028)
                     /locus_tag="OO566_003206"
     CDS             complement(5306..6028)
                     /locus_tag="OO566_003206"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6606389.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPILGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNMLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6030..7205)
                     /locus_tag="OO566_003207"
     CDS             complement(6030..7205)
                     /locus_tag="OO566_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR6606390.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYLLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7232..8848)
                     /gene="bglH"
                     /locus_tag="OO566_003208"
     CDS             complement(7232..8848)
                     /gene="bglH"
                     /locus_tag="OO566_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR6606391.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8934..10331)
                     /gene="bglB"
                     /locus_tag="OO566_003209"
     CDS             complement(8934..10331)
                     /gene="bglB"
                     /locus_tag="OO566_003209"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6606392.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10347..12224)
                     /gene="bglF"
                     /locus_tag="OO566_003210"
     CDS             complement(10347..12224)
                     /gene="bglF"
                     /locus_tag="OO566_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6606393.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(12358..13194)
                     /gene="bglG"
                     /locus_tag="OO566_003211"
     CDS             complement(12358..13194)
                     /gene="bglG"
                     /locus_tag="OO566_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6606394.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         3548 a         3373 c         3080 g         3438 t
ORIGIN      
        1 atttcaggaa ggagtgtccg ttaaactcag gctacctcag ggcgctggac aatgtgttac
       61 aggagctggg gcgcgaacgc acgattgcta tgagcctgcc ggaattcgag cagtcactgt
      121 ttatggcagc gcaacccgac aatctgctac tggcgaccgc gccgcgctac tgtcagtact
      181 acaatcaact ccatcaactg ccgttggttg ctcttcctct cccgtttgac gaaagccagc
      241 aaaaaaagct ggaagttcct tttaccctgc tgtggcataa acggaacagc cataatccga
      301 agatcgtctg gttacgggaa accattaaaa acctttacgc gtcgatggca taaccgaatc
      361 gtatgaaacg cgacccaatt tcacaataaa atgtaaaaaa gttgtaataa gcttgtctga
      421 atcgaacttt tagccgcttt agtctgtcca tcattccagt aaatgattac tcttgtattc
      481 ataatggacc attaagcatg gagcgaaaaa tggcgactca ctttgcccga gggattttaa
      541 cggaaggaca tctgatttct gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca
      601 ttccccctca tcgtcaaagc cgttttctgg cgtccagagg tttactcgca gaactgatgt
      661 tcatgctgta tggcattggc gaattgccgg aaatcgtcac cctgccgaaa ggtaaaccgg
      721 ttttcagtga taaaaatttg ccttcgtttt ctatttccta tgccgggaat atggttggcg
      781 tggcgttaac aaccgaaggt gaatgtggcc tcgatatgga actacagcgt gcgacgcgcg
      841 ggtttcatag cccacacgcg cccgataacc acaccttttc cagcaatgaa tcgctatgga
      901 tcagtaaaca aaacgatcct aacgaagcgc gggcgcagct cattacgctg cgccgaagcg
      961 tgctaaaact aaccggtgat gttttgaatg acgatccgcg cgatctgcag ctgctgccca
     1021 ttgccggacg cctgaaatgt gctcatgtaa atcatgtaga agcgttatgc gacgcggagg
     1081 acgtgctggt gtggtccgtg gcggtcacgc ccgcgattga aaagctcagt gtctgggagt
     1141 tagatggcaa acacagctgg aaaagcctgc cggatattca cagccgcgcc aacaatccta
     1201 ccagccggat gatgcgtttt gcccagctct ctaccgtgaa gtctttttcg ccaaattgat
     1261 agacaaccac aggagtcatt atgtctgaaa aattgcaggt ggttacgtta ctggggagcc
     1321 tgcgcaaagg ctcatttaat ggcatggttg cacgtaccct gccgaaaatt gctccggcga
     1381 gcatggaagt caatgcgtta ccttccattg ccgacattcc cctgtatgac gctgacgtac
     1441 agcaggaaga aggttttcca gcaacggttg aagctctggc ggaacagatc cgtcaggctg
     1501 acggtgtggt gatcgtcacg ccggaatata actactcggt accgggtggg ctgaaaaatg
     1561 ccatcgactg gctttcccgc ctgccggatc aaccgctggc cggtaaaccg gtgttgattc
     1621 agaccagctc aatgggcgtg attggcggcg cgcgctgtca gtatcacctg cgccagattc
     1681 tggttttcct cgatgcaatg gtgatgaaca agccggaatt tatgggcggc gtgattcaga
     1741 acaaagttga tccgcaaacc ggagaagtga ttgatcaggg tacgctggac cacctgaccg
     1801 ggcaattgac cgcatttggt gagtttattc agcgagttaa gatctaaata aaaaacccgc
     1861 cagtaatcat gcatggcggg tttttaatgc gctatcgatt ttaatgagcg tcgataaata
     1921 caatcttcag gataaacagc agcgcaacga tgattacgca cgggctaagg tcacgcagac
     1981 gcccggtacc aatcttcatc acgcagtagg agataaagcc cagcgcaata ccttcggtaa
     2041 tcgaaaagct gaacggcatc atcacggcgg taataaacgc cggaacagat tcagtaagat
     2101 cctgccagtt cacgcgagca agacttgagg tcatcaacac gccaacgtag atcaacgcgc
     2161 cagctgcagc gtagcctggc accattcccg ccagcggcga cagaaagata accagcagga
     2221 acagcagacc aacaaccact gccgtcagac cggtacgacc gccaaccgat acgccggaag
     2281 aggactcaat ataagccgta acggaagaag taccgataaa cgaaccggtc acggaagaga
     2341 tactgtcgac atacagcgcc tgcttcatgc gcgggaattt ccccttctca tccgccagac
     2401 ctgctttatc ggtcacgcca atcagcgtac cggaggagtc aaacaagttg accaacatga
     2461 aagagaaaat cacccctgcc agcccgaggt taaacgaccc ggctaaatct acatgaccca
     2521 caactgtcat tacgctcggc ggcgcagaaa cgatgccatt gtagtgcaca tcacccagca
     2581 tccagcccag cagcgtcgtc accacgatag aaaccagcac cgctgcgtga atgttgcgcg
     2641 aggccagaat agcaatgatg aaaaagccga ggatacccag aagtacgctg tgagaagtca
     2701 gattaccgat gctcaccagc gtttccgagt tagcgacaat cacacctgcg tttttcagcc
     2761 ccatcatgcc gataaacagg ccgataccgc tggtaatacc cacacgcaga cttaccggaa
     2821 tgttagcaat catccagtaa cgaacgcgga aaatggtcaa aagaagcagt ccgacagcgc
     2881 cccagaagat tgcgcccata ccgatctgcc acggcagccc cattgcctgg acaaccacaa
     2941 aagcgaaaaa ggcgttaagc cccatcgcag gagccagcgc caccggaagg tttgcaaaca
     3001 atcccatcat aatgctaccg aaagcagcaa tcagacaggt tgttacgaag acggcactgg
     3061 tgtccatgcc agcaacgccc agaatttgtg ggttaacaaa tacgatgtag accatcgtca
     3121 ggaaggtggt gaacccggca atcacttcag ttcgtgccgt agtaccatgt tcgcgcagtt
     3181 taaaaacgcg ttccagcatc ctctgaccag aagtctgggt ggtatgttga tgactcatta
     3241 tctatttccg aacaaggagg gaaaatccgt cgctatcgta taccaaaatg cgacaatagg
     3301 cgcgtttgtg agagactttt ttattggatt tacttatacg gcaacgattg cgttgcgcaa
     3361 atcggctcta cgaaaacgtt aaactgatta aaaaggaaag gcatgtcccg gatagaagcg
     3421 gtatttttcg actgcgacgg tacgctggtc gacagtgaag tcatttgctc ccgcgcatat
     3481 gtaacgatgt ttcaggaatt tggtattacg ctcgatcctg aagaggtatt caaacgtttc
     3541 aaaggtgtaa aactgtacga aattatcgat attgtttccc ttgaacatgg tgttacgtta
     3601 gcgaaaacag aagctgaaca cgtttaccgt gcagaagtcg ctcggctgtt cgattcagaa
     3661 ctggaagcca tcgaaggggc tggagcgctc ctgtcagcga tcactgcgcc aatgtgtgtg
     3721 gtatctaacg gcccaaataa caaaatgcag cattctatgg gcaagctgaa tatgttgcac
     3781 tacttcccgg ataaactgtt cagcggctac gatattcagc gctggaagcc agacccggcg
     3841 ttaatgttcc atgcggcaaa agcgatgaat gtaaatgtag aaaactgcat tctggttgat
     3901 gactcagttg ccggtgcgca atctggtatc gacgcaggta tggaagtgtt ctacttctgc
     3961 gccgacccgc acaataagcc gatcgttcac ccgaaagtca ccacctttac ccatctttcg
     4021 cagttaccgg aactgtggaa agcgcgtggt tgggatatta cggcatagtt cttcacactc
     4081 ccttcactta ccccgcttaa attggcgctc aaaggtaagt aaagggagtt tgatatgtct
     4141 gtttcacgtc gggtaataca tcacggactt tattttgcgg ttttaggacc gttaattggt
     4201 gttctgtttc ttgtcctcta catattcttc gcaaaagaac cgctggttct tttggtaata
     4261 atacaggtac ttccattgtt tctcttattg tcgattacta cgggagctat tcctgcattg
     4321 ttaaccggcg tgatggtcgc ctgtctgccg gaaaagatcg ggtcacagaa aaattatcgt
     4381 tgtctggctg gtggcatagg tggcgtcgtt atcaccgaga tctattgtgc agttattgta
     4441 catattaagg gcatggcttc ctcggagttg tttgaaaaca ttctttctgg tgacagtctc
     4501 gttgtccgca tcattcctgc attgctggca ggtgtggtga tgagcagaat cattacccgt
     4561 ctgcccggat tggatatttc atgtcctgaa acagactctt taagttaagc gggatacttt
     4621 atctttgggc tactcaaaag cagacaggat gttactatga ctcaaaatat caggccgtta
     4681 ccccaattca aatatcatcc caagccactg gaaacaggcg catttgaaca ggataaaacc
     4741 gtagagtgcg attgctgtga acaacagacg tcagtttatt actcgggtcc cttttattgc
     4801 gttgatgaag ttgaacatct ctgtccgttg tgtattgcgg acggttctgc tgctgaaaag
     4861 tttgcaggta gttttcagga tgatgccagc atagaaggtg ttgaatttga gtatgatgaa
     4921 gaggacgaat ttgccggtat taagaacaca tatcctgatg aaatgctgaa agagttggtt
     4981 gaacgcacgc caggttatca tggatggcaa caggaattct ggcttgcgca ttgtggcgat
     5041 ttctgtgctt ttatcggcta tgtgggctgg aatgatataa aagatcgcct cgatgaattt
     5101 gccaaccttg aagaagattg tgagaatttc ggtattagaa attctgatct agctaaatgc
     5161 ctgcaaaagg gtggtgattg tcagggttat ctcttccgct gtctccactg cggcaagctg
     5221 agactgtggg gtgatttttc gtagttattt aaataatgag aacaggccgg agcgtaattc
     5281 atacatccgg ccttatttct tcagcttaac cgaacgctaa ttctgcggca gcggctttat
     5341 cagcgattac catcagcgaa ggatgcagct gcaacacgga agcaggaaca tcttcggtaa
     5401 ccggaccttg cagcatattt ttcagtgcct gcgctttacc tgcgccactg acaattatca
     5461 gcaagttttt cgccgccatg atgcttttcg gccccatcgt cacgtagctg tcaggcacta
     5521 acgaaaagtc gccgcccaac tcaccatgcg ccacaatatc aaccatctct cccaggatcg
     5581 ggaactccac ggtttgctca tggaaatggg tcgtattcgg caaattgcca caaaaatgac
     5641 catctgcacc taatcccagc accaccaaat ccaggccgcc ttcacgcgcc agtttctgat
     5701 catgctcgcg gtagttatca atagtgagct tctggatatt ctcttctttg atccccgcag
     5761 gggtgaaaaa cagattacgc agattggtaa tcgttacgcc ttctccctct ttgccgcgaa
     5821 agggaatttc atcgaaatta tagaaatagc agttatcgta ccaaggctta cctttaacca
     5881 gggtggtgag gtattcatac atgcctttgg gcgtgctacc ggcggtaatt gccaggttaa
     5941 cacgacgcgt cttcgacata taaccaagca gatggtgtgc cgcgacacgg ctcatttcct
     6001 ggtaatcttc ggtaatgatt aatttcatct taatgcctta ttttaaattt ctgtacgaag
     6061 gctgcatagg caggccgcca gacatccatt tcatggttca ggccgggata ttcctggtaa
     6121 tcaaacttaa tttttttctg ctcaagctca gttttcagcc cggcgatatc cttgccggtt
     6181 acgacatctt tatcaccaac gaccacagta aaattacgta gttgctggtt aatagctgcc
     6241 ggatcgttca gccgggccgc gacaccttca tccggtacgg ttgtcgtggt aacaccactg
     6301 aatgtggcca gccagccaaa gctttccaga tgattcattc cggaaaccag cgcctggtac
     6361 ccgccttgtg aaagccctgc cagcgcgcga ccatcggcat ctttacggac actaaaacgc
     6421 ttgctaatca gcgggataat atcgttcatc agttcgcgat ctgccgcttt agcattcagc
     6481 agataaaaga ctttacgtct ttcctgaggc acgaaatctt cgggaataat gcccttcgca
     6541 tcggtttcgg tatccgggat caccaccagc atcggtttaa ttttcccttc agccagcagg
     6601 ttatccatga tttgcgggat acgcccctga tcgatagcgg aacgtccagt atcaccaaaa
     6661 ccgtgataga aatagagcac tggcaaaggc tcgcccatgc cggtgtatcc tggcggggtc
     6721 cagacataca tctgacgttc agattgcaat gcgttggagt ggtaagttat ggcgatcaaa
     6781 tcaccatgcg caacagaacg cgtatccaga taactgccta gcaccagaat catactggag
     6841 ttaacctggc gctgaggctt agtcattgcg gtgcctgtat caatgctgcg tacaccatca
     6901 acattgaaaa aatactcgta caggttgcct ttcaggatag gtgtgcgcca cgaccagact
     6961 cctgcttcgt ctttggtcat cgggtgaata ttgtccggaa ccggaacacc caccactaca
     7021 gagacatttt ttgccccagg ggcaaagtag cgaaaggtca cgctgttatc ggcattgacc
     7081 tgagtcacat actgtttcac cgggatagtg ggtgccgggc tggctggcat atccgctgct
     7141 atggcccact gagcagaaat accgctggca atagccagcg ttaaagcagc aatttttata
     7201 ttcatcgtaa tattcctttc attaagttcc attaccacca gatttcagcc tgggcaccga
     7261 cagcaaactg gtcgtcttta ttgtcttcga aggtgaagcc atccagttcg ttttccaggg
     7321 ctttgatata cgtggcgtag aaacgaattt ccagacgcga ggtcaacata ctggtattga
     7381 ctttaaaggt atggaagagc gtggttttat aaccagactc attaaattta ttactattcg
     7441 catctttgtt ttgctgggtg aaatagccca gttcaacacc tgtctggtta tattggtccc
     7501 aaatataggc cgggcgaaca accgcacgaa tagattcgaa atcagagtgt gcgcctgttt
     7561 cgtagctata aatatcgtta ccgaaggagt aaacaatcgc gttagccaca ataaaatgat
     7621 cgccgatata ggcttcgccc tgcgaggtca gacgaactgc tgttccgccg gtgtgatcac
     7681 cataataacg accattaaag gtggtaaatg gacttgcgcc agcataacgg ccaaagttcc
     7741 tggcgataga gttattcgca accaggaagg agaattcgtt gaagccacct ttatcaaatt
     7801 tctgcgttaa agatgtgccg aacatccagg tatctttcca gtcataatac ccgttattat
     7861 cctgattatc tttttccgat gcgctttcgt ttgccgtgac ataacgacca ctcaccatta
     7921 aggtcgcttt atcccataac gggatatctt tatagcgtaa atcaatagta tgggtattaa
     7981 tctgctgttt gttttgcagg ctgcgatcgt aatcatcgac atcttcgcga accagcgcga
     8041 tatcaatttt acccggaccg actttccagt tttccagacc tacacccgct gcggcatcag
     8101 tacgctgcgt tttccagtcc agcatctgga tttcaatttt cggcgcaccg tgtttaccca
     8161 cccagaaatc agcctctggc gcaaagggca ggaaaccttt ggtggtaacg tacatatcgg
     8221 agaactgcat aaagttctcg ccaccggcgt tatcgccaaa ccagccggta gagtactgct
     8281 gaccaacgtt accatccatc atcacaacgg catcaacccg tttgccgttt tcgttgtaga
     8341 cacgttgttt taactgcaaa tcaaaccagc cggagtattc gttaccaaag cggcccagag
     8401 aaccaatcgc ccatgattta ggtgaaccat gagaggcggt cccccaacca gaacggtaat
     8461 agccgttgta actaaagcca atttcatctt tcacaaactt actgaaatct ttcagagtca
     8521 tggaagaata aatcgagcct gtctcactgg catttttctc ttcatttttg accagtacag
     8581 catcaggttt tgcggcacta ctggtcggga acggattggc ggcataccct tgatcattcg
     8641 tacttacgct acgattcacc gtcgctggcg tatatttttt cttctcttca tctttatact
     8701 ttttgagttc gctttgcgtt tctcttaacg ctttttctaa taactcaagg cgttgttcca
     8761 ccgttaatga ttcagccaat gattgtgcag aaaaggctaa cggtgccatt aataagatgg
     8821 cagaggtaat aagatttcgt ctaaacataa tatcccttta tggtgcaaag aaagaattaa
     8881 cgtatcgcat ccagactgtt ctgaatgcga cgataattaa ggtgctttat tggttatttt
     8941 tttaatgaca gcccccgcgt tttgatcact tctgcatacc atccgaagct tttcttacgc
     9001 gttcttgtga ggcttccttc gccattatca tcacgatcca cataaataaa gccgtagcgc
     9061 ttagacattt gtgaatgaga tgcactgact aaatcaattg gcccccaact ggtgtacccc
     9121 ataatatcca caccatcggc aatcgcttca tttacctgta ccaggtgatc gtttaaatag
     9181 gcaattcgat aatcgtcctg tatcgaacca tccgcttcaa cgctgtcttt tacgcctaat
     9241 ccattctcga caataaataa cggtttttga taacgatccc aaagcgtatt taacagaacc
     9301 cgtaatccaa ccggatcaat ttgccacccc ctctctgaac ttttcagatg cggattgggg
     9361 atcatattca gtatgttgcc ctgcgcattt ttattaatgc tttcgtcgtg ggaaacacaa
     9421 ccagtcatgt aataactaaa agagatgaaa tctacagtat gttttaaatc ttctgcgtca
     9481 ctttcagtca tctcaatggt gatattgtgg tcgcggaaga aacgctgcat atagccggga
     9541 tactggccac gcgcctgaac atcaccaaag aacatccagc gccggttctc ttccatggcc
     9601 tgcaacatat cctgtggctg gcaggtgagg gggtaaacca gcccaccgag aagcatattg
     9661 ccgattttcg cttcggggat caggctatga taggctttaa ctgcccgcgc actggcaacc
     9721 agttgatgat ggatcgcctg ataaacttcc gcctcgccac tctcttctgc cagccccacg
     9781 cccgtgaatg gcgcgtgtaa cgacatattg atttcattaa acgtcagcca taacgctact
     9841 ttatgttggt agcgggtaaa gaccgtgcgg gcgtaatgct cgaagtgatc gatgaccgcc
     9901 cgattagccc aaccgccgta gtttttcacc agcccatata gcatttcgta atgggataac
     9961 gttaccagcg gcttgatccc cgcctgcgcc atttcatcaa acagccgatc gtaaaacgct
    10021 aaccccgctt cattcggttc ggcttcgtcg ccctgaggga aaattcgcgc ccaggcaatg
    10081 gaaatacgca gacaggtgaa gcccatctcg gcaaataacg cgatatcttc cgggtaacgg
    10141 tgataaaaat cgatggcgac atctttgata ttctctttcc ccaggatgcg cggttccatt
    10201 tttcccatta cgccatgagg ctgtaaatct gaggtcgtga tccctttgcc atcttccagc
    10261 caggcacctt ccacctgatt ggcagctgtt gcgccacccc aaagaaatgt ttctggaaat
    10321 gctttcgtaa ttaactcctt ttatcgttag cgaatgatgg ataacagcgg ttcacctgcg
    10381 cttatctgcg ccgtgccgtg gggtaatacg tctgtaaaat catcgctatt actgattaat
    10441 accggcgtcg tcagatcaaa tccggcctcg cgaatagcag ggatatcaaa agaaatcagc
    10501 cgatcgcctg tattgacctt gtcacccacg ttgacgtgag cggaaaagaa tttgccgtcc
    10561 agttttacgg tgtcgatacc gacatgaatc aggatctcca caccatcatc tgactcaatg
    10621 ccaatggcgt gtaatgtggc gaacaacgaa gcaattcgac ccgcaaccgg agaacgcact
    10681 tcaccaaccg agggcagaat ggcgatacct ttacccagca ggccactggc aaacgtggta
    10741 tcagcgacgt gaatgagcgg cacaatctct cccgtcatcg gtgaacagat accgccctgc
    10801 tcaggtggtg taataacctc tggtgttttc tcttgcgggg caccctgcgc tggctgacgt
    10861 ttactggtga tgaaatgaag catcaacgta ccaacaaatg cgcaacctat ggcaatgaca
    10921 ccgccaataa cgctggccca gacggtgaaa tcaatccccg ttgacgggat ggtctgcatg
    10981 aaggtgaaaa tacttggcaa accaaaggag taaactttcg tttgcgcgta gccaatgatg
    11041 gtggccccca atgctccact gatacaggcg ataacaaagg ggtacttacg cggcaggttg
    11101 acgccatata ccgctggttc ggtgatacca aacagacccg tcaacgccgc tgatcccgcc
    11161 accacttttt tctgcgcatc gcgttcgcag aggaagacgc cgagcgccgc cccgacctgc
    11221 gccataatgg cgggcattaa cagcgggatc atggtgtcgt agcccagcac ggtgaagtta
    11281 ttgatacaca gcggcaccag gccccagtgc agtccgaaca tgacgaagat ttgccagaag
    11341 ccgcccatta ccgcgcccgc aaatgcagga accgcctgat aaagccagag ataaccggcg
    11401 gcaatcattt cgcttatcca ggttgatagc ggccccacca gcagaaaggt gacgggtgtg
    11461 ataaccatca gacatagcaa tggtgtgaag aaatttttga ttgccgacgg taaccacgca
    11521 ttaagtcggc gttccagaat gctgcacaac caggcagaaa aaataatggg aataaccgat
    11581 gacgagtaat tcaacaatgt gaccggaata cccaggaaat ccagccccgg cgcatccgct
    11641 ttttgcccgt tctcgaaggc agtcagaatt aatggatgca ctaacgctcc accaatcacc
    11701 atggcagtaa atggattacc gccgaagcgt ttgcccgcgg tgtatcccag gattatcggg
    11761 aagaacccaa acaaggcatc actggcgctg aataaaatta aataagtacc actttgttcg
    11821 gtcgtccact gaaaagtgag cgccagagcc agcatacctt tcaggatccc ggttgccgcc
    11881 atcagaccga tcagaggcgt aaaaatacct gaaataacat aaacaaagcg gtttagcaga
    11941 ttacctttct catcattttc cggtgcctgt tgcgcttttt cgccaaggcc tgccacgctg
    12001 ttaaccgcca ggaagacatc ggccacatgg ttacctatga ccacctgaaa ctgaccaccg
    12061 ctttccacca ccataataat accgggggtc tttttcagta cctcagcttg cgctttgctt
    12121 tcatccttta acttaaaacg taatcgcgtt gcacaatgca tcagactcac aatgttatct
    12181 gcgcccccga ctcctgcgac tatttttctg gctaactccg tcataacttg ccctctaccg
    12241 ctttgcggca aaactccaaa aaaaaacctg aaaaaaacgg cctgacgtga atcaagcaat
    12301 ttttttcagg ttttgcccgc ttagtgcggt aacaatcctt tactcagtaa taatatttca
    12361 gtgttctttg cgcacgcgct ctatatttat ggctaaaaac ataatctctg cgggtgaaat
    12421 tttacgttga tactgcaaac caataaaaat ggcgatccgt tccgcacatt gccatgcttg
    12481 cgggtaattt tgttttactg cttgttgtaa tgattcatca ctatcgttaa ttgaagcatg
    12541 ttcaagaata cgccaggata aaaacttcag atgtgtaacc agtcgctgat aactcaagct
    12601 ttcttcctgg taattaaggc tgaactgaaa ttttattaat tgcagcattt cgcgcattaa
    12661 ctgcgtgaca cctgcaacat cctccatatt tccgctcatt tgggcactga ccagatgcat
    12721 ggcaataaag cccacttcat ctttcggtaa ctgcacgccc aaccgtttat caataatggt
    12781 taacgcttct tcccctagct ggaactcttt cgggtaaagc cgctggatat cccacagcaa
    12841 cgggttgggc agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt
    12901 tagcgagata taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag
    12961 acgatcacag gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc
    13021 gttcagttca tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc
    13081 gccagcgcgt ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc
    13141 atcaataacc accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag
    13201 gacctttttt ataaacaaaa aaacccgact tcaccagtat tctctggtta tgtcaggttt
    13261 tgcctgcgaa tgcagtaaca atccagtcat ttattaatgg tttttataac gaacatccag
    13321 gttcggaaat taatttaatt gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat
    13381 gaccatgctc gcagttatta actttgtgta attttaggaa tttataaagt tatatataa
//
LOCUS       DAJTXY010000110        13121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTXY010000110 DAJTXY010000000
VERSION     DAJTXY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.110"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(506..1597)
                     /gene="ychF"
                     /locus_tag="OO566_003212"
     CDS             complement(506..1597)
                     /gene="ychF"
                     /locus_tag="OO566_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6606395.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1714..2298)
                     /gene="pth"
                     /locus_tag="OO566_003213"
     CDS             complement(1714..2298)
                     /gene="pth"
                     /locus_tag="OO566_003213"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6606396.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2576..2854
                     /gene="ychH"
                     /locus_tag="OO566_003214"
     CDS             2576..2854
                     /gene="ychH"
                     /locus_tag="OO566_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6606397.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2909..4588)
                     /gene="dauA"
                     /locus_tag="OO566_003215"
     CDS             complement(2909..4588)
                     /gene="dauA"
                     /locus_tag="OO566_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6606398.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4713..5660)
                     /gene="prs"
                     /locus_tag="OO566_003216"
     CDS             complement(4713..5660)
                     /gene="prs"
                     /locus_tag="OO566_003216"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6606399.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5811..6662)
                     /gene="ispE"
                     /locus_tag="OO566_003217"
     CDS             complement(5811..6662)
                     /gene="ispE"
                     /locus_tag="OO566_003217"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6606400.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6662..7285)
                     /gene="lolB"
                     /locus_tag="OO566_003218"
     CDS             complement(6662..7285)
                     /gene="lolB"
                     /locus_tag="OO566_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6606401.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPTNMELTDGGQRIKLKMDNWIVK"
     gene            7499..8755
                     /gene="hemA"
                     /locus_tag="OO566_003219"
     CDS             7499..8755
                     /gene="hemA"
                     /locus_tag="OO566_003219"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6606402.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8797..9879
                     /gene="prfA"
                     /locus_tag="OO566_003220"
     CDS             8797..9879
                     /gene="prfA"
                     /locus_tag="OO566_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6606403.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9879..10712
                     /gene="prmC"
                     /locus_tag="OO566_003221"
     CDS             9879..10712
                     /gene="prmC"
                     /locus_tag="OO566_003221"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6606404.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10709..11101
                     /locus_tag="OO566_003222"
     CDS             10709..11101
                     /locus_tag="OO566_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6606405.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11105..11914
                     /locus_tag="OO566_003223"
     CDS             11105..11914
                     /locus_tag="OO566_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6606406.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11950..12804
                     /gene="kdsA"
                     /locus_tag="OO566_003224"
     CDS             11950..12804
                     /gene="kdsA"
                     /locus_tag="OO566_003224"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6606407.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12953..13060)
                     /locus_tag="OO566_003225"
     CDS             complement(12953..13060)
                     /locus_tag="OO566_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6606408.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3275 a         3303 c         3395 g         3148 t
ORIGIN      
        1 cgcttgatgt atctgcataa caaaaacaat atgcgccatt tttgcattac taataagaaa
       61 aagcaaaaaa catcgatatt catttttggt caatggattt tctggcctat cgttattatt
      121 gtgctactgg ttatcatttt catcttgcca ggatcgcacg taatcacgtt attcggggac
      181 tttatctgcg ggatattacc cttcaggtaa gtccttttct cgccagcata ttggagcgag
      241 atcaatattg atcagctgtg aagagggctg atgtgtagtg gaacaaaaac tgacgttata
      301 tcactgctaa tgagatatca gtcatctgaa cacttatgtc tttaattatt ctcgtggttc
      361 actgtaggca ataagcacaa aagtgtagga tgttacaaga atgattagga ctcggtgaaa
      421 tgaaaaatcc acgcaattgc gtggatttta tatacttttg cgctcttcat gagatttagc
      481 gaaacctcat gagacaataa attaattaga cgttgaaaag gaagttcatc acatcgccat
      541 ctttcacgat gtaatcttta ccttctgcac gcattttgcc tgcttctttc gcgccttgtt
      601 cacctttgta agtgatgaaa tcttcaaacg agatggtttg tgcacggata aagccttttt
      661 caaaatcagt atggattttg cccgctgcct gcggcgcggt tgctccaacc ggaatggtcc
      721 atgcacgcac ttctttcacc ccagcggtga agtaagtttg caggttcagc agcttataac
      781 cggcacggat cacacggttc aggcccggtt cttccagccc aagctcctgc ataaactcgt
      841 cacgttcttc gtcgtccagt tcggcaatgt ctgcttcaac agcagcacaa accggaacca
      901 caacagaacc ttctttcgcc gcgatttcac gcacctggtc aagatatggg ttgttttcaa
      961 aaccgtcttc gttgacgttg gctatgtaca ttgttggttt cagcgtcagg aagctcaggt
     1021 aacgaatagc cgctttctct tcagcgctta aatccagcgc gcgcagcata cctgcgtttt
     1081 ccagctgggg caggcatttt tccaggaccg ccagctcagc tttcgcgtct ttatcgccac
     1141 ctttggcttt cttctgtacg cgatgaatcg cacgttcgca ggtgtcgagg tctgccagcg
     1201 ccagttcggt gttgataact tcaatatcgt cagccgggtt aactttgccg gaaacgtgaa
     1261 tgatgttgtc attttcaaag cagcgaacaa cgtgaccgat cgcttcggtt tcacggatgt
     1321 tggtcaggaa ctggttaccc agaccttcgc ctttcgatgc gccttttacc agaccggcga
     1381 tatcgacaaa ttccatggtc gtgggaagcg tacgttgcgg ttttacgatt tcagccagtt
     1441 gatccaggcg agggtcaggc attggtacga cgcctgtgtt cggctcaatg gtgcagaatg
     1501 gaaagttggc cgcttcaata ccggctttgg tcagcgcgtt gaacagggta gatttcccga
     1561 cgttgggcaa accgacgata ccgcatttga atcccatgat ttaaatcacc ttaatatctt
     1621 aataatcaac ctgttattgc taacagattg cagaaatgga aataactttg cctattatac
     1681 acggcactcg gcaaaaatgc cgcagacaac gacttattgc gctttaaagg cgtgcaatcg
     1741 gttcgttgct ttggtcaagc catctgtaaa ccacatttca gtacaacgcg ccgcttcgtc
     1801 gatggcttca tcaattaact tctgttcact aacaggcggt ttgcctaaca caaaaccgac
     1861 aactttattt ttatcgcccg gatgaccgat tccgatgcgt aaacggtgaa agttagggtt
     1921 attacccaat ttactgatga tgtctttcag tccattgtga ccaccatggc caccgcccaa
     1981 cttaaatttg gcgacgccag gaggcagatc cagttcgtcg tgggccacca gaatttcgtc
     2041 cggattaatg cggaaaaaac tggccatcgc cgcaacggct ttgccgctga gattcataaa
     2101 tgtagtcggg actaacaggc ggacatcttc gcctccaagg gtgactcgcg aagtataacc
     2161 aaagaattta gcctcttcgc gcagcggagc gcgcaaacgc tctgccagta agtcaacgaa
     2221 ccaggcacca gcattatgtc gcgttgcggc gtattcagca ccggggttcg ccaggccgac
     2281 aatcaattta atcgtcacgt ttttttgtcc tgagtgtgta cataactggc gcgtagttta
     2341 ctggttgcgg ccccgcttga caaaaaactg cgtatcaaat gcagataacg taataattgc
     2401 ctgagtggac tattagaaag tcaaggtgtt caggcgttta tttgtaaagt tttgttgaaa
     2461 taagggttgt aattgtgatt acgcccgcac ataacccaca gggtgttgtc tatactttac
     2521 acataaggaa gaggggtatt ccctgttaca acccagaaag ttccggaggt gacatatgaa
     2581 acgcaaaaac gcttcgatac tcggtaacgt gctcatgggg ttgggtctgg tggtaatggt
     2641 ggtcggcgtg gggtattcaa tcctcaacca gttaccacag tttaatatgc cccagtattt
     2701 cgcacatggt gcagtgctaa gtattttcgt cggtgccatt ctctggctgg cgggtgcccg
     2761 tgttggcggg catgaacagg tgtgcgaccg ttactggtgg gttcgtcact atgacaaacg
     2821 ttgccgccgt agcgataatc gccgtcatag ctaacaaata atgcagtttg ctgaccagtc
     2881 agtttgcgct gactggtcaa tctcgtactt ataaatccgc catcgccgcg cgacgattcg
     2941 ggaagaacgc gaggcgtccc gggatcggtt gaatgcctgc gcgagccata gttcgcagtg
     3001 gctggaattc cacgttgcac acgcgcagtt cacatccttc gggcagacgc ttcacaaaac
     3061 gctggaacgc atcaagacca ccagcatcaa gtaccggaac ggcatcccac ttcagaatca
     3121 caatccgttt gccttcaaga cgtgactcca ggtccgtgaa taagccttca gcagcagcaa
     3181 aaaacagcgg gccaataacg cgcagaacca ggacatcgtc tggaacatct acgactaccg
     3241 gtgccaggcg agtcatacgt gcgatacgac gcataaacag cagcgatgcc agcacgatcc
     3301 ccacgctgat ggcaataacc atatcaaaca gcacggtcag tgacatacac agcagcatga
     3361 cgatgatgtc atctttcggc gcatgacgca gcaagtcgac cactttgtgc gcttcactca
     3421 tgttccacgc caccatcaac agcagggctg ccatggcgga aagcggcagc caggagagca
     3481 gtggtgccag taccagcagg gcgagaataa ccagaataga gtggatcacc gccgagatag
     3541 gggaagttgc cccggcacgg acgttagcgg cagaacgcgc gatggcagca gtagcggtaa
     3601 taccaccaaa gaatggagcg atgatattcc ccagcccctg accgaccagt tcactattcg
     3661 ccttgtgctt agtcccggtc ataccatcca gtaccacagc gcagagcaga gattcgatcg
     3721 ccccgagcat tgccattgag aatgccgcag gcagcagggt gcgaatagaa tcccaggtta
     3781 gcgtgaattc tgaattaggc agatcccacg gcagcaccag ttgcggcagc agttgcggaa
     3841 taccgttacc ctgagaacca tcggccagga cgtagtggaa ttgcgaaccg atggtagcaa
     3901 catgtccgcc gagcaggtta acaatcccca tcaccgcgca accagccagc aaggccggaa
     3961 ggtgaccggg taaacgaatg cccagacgcg gccagaaaac cagaatgcca agcgtcacaa
     4021 tgccaatggc agcatcaccc acattaatgg tcggcagcgc cataaataat gcgccgactt
     4081 tttgtagata atgttccggg acatgggcca tttgcagacc gagaaaatct ttaatctgca
     4141 tggtaccgat ggtgatcccg atacccgagg tgaaacctaa ggtgacggaa accggaatat
     4201 actcaatcag acgaccaaag cgtgccagcc ccatcagaat caaaaagatt cccgacaaca
     4261 aggtcgcaac cagcagtcct gccagtccaa actgttgcga aacgggatag agaattacca
     4321 caaatgccgc agtcggaccg gaaacgctaa agcgtgaccc acccgttaga gcaatgacaa
     4381 tccccgcaac agctgcggta tataaaccgt actggggtgc cacaccacta ccaatagcca
     4441 acgccatcgc cagcgggata gcaataatcc cgacggttat cccggcaatc aggtcacggg
     4501 taaaccgtgc ggcagtatat ttttctttcc agcaagcgtc gatcagagcg cggaaaggca
     4561 tcacatgtga ggaaaatatt ttgttcacaa taatgtttca tccgtgagcg catcatctgt
     4621 caactaaatg gcaggtgaag gaggcatagg tcatacaaat ggatattaca gacaaaaaaa
     4681 cccgccgcag cgggtctttg agccgggttc gattagtgtt cgaacatggc agagatcgat
     4741 tcttcgttgc tgatacgacg aatcgcttcg gccagcatac ctgacagggt cagagtacgc
     4801 acgttcggca gtgatttgat ttcatcgctc agcggaatgg tatcgcagac aacgacttca
     4861 tcaattacag agttacgcag gttgttcgcc gcgttgccag agaagatcgg gtgagtcgcg
     4921 tacgcaaata cacgtttagc accacgttct ttcagcgctt cagcagcttt acacagcgta
     4981 ccgccagtgt cgatcatatc atcgaccagt acgcagtcac gacctgcaac gtcaccgatg
     5041 atatgcatca cctgggaaac gttcgcacgc ggacgacgtt tgtcgatgat tgccatatcg
     5101 gtatcgttca gcagcttagc gatagcgcgg gcacgcacaa cgccgccgat gtccggagaa
     5161 accacaattg ggttatccag attcagctgc agcatgtctt ccagcaggat cgggctacca
     5221 aatacgttat caaccggaac gtcgaagaaa ccctgaatct gttcagcgtg cagatccact
     5281 gtcagcacac ggtcaacacc gacgctggag aggaagtctg caaccacttt cgcagtgatt
     5341 ggtacacgag cggaacggac gcgacggtcc tggcgcgcat agccaaagta ggggataaca
     5401 gcggtgatac gacctgcgga agcacgacgc agggcatcaa ccataacgac taattccatc
     5461 aggttgtcgt tagtaggggc acaagtggac tggatgatga aaatatcacc accgcgtaca
     5521 ttttcgttaa tttgtacgct gacttcgccg tcgctaaagc gacctacagc ggcgtcgccg
     5581 agtgaagtgt acaggcggtt ggcaatacgt tgtgctagta ccggggtggc gttaccagca
     5641 aaaagcttca tatcaggcac gagaagaacc tcaggcatgc gtccattggt ggaaagaatc
     5701 tgccgaaaac tgtgcgggcc aggcatgatc ctttccaggc ggtgtattaa agagcgcgat
     5761 gcaacgtctg gaacaaggtg acgttgtcac cgaaactcag cttgcccggc ttaaagcatg
     5821 gctctgtgca atggggaaag attaacgcct ttcgccacaa agccattgag ccattccggg
     5881 gcttgctcta gcacctggcg ggcttcagac tctgtatcaa attcagcaaa gacacaggcc
     5941 cctgtcccag tcaggcgcga cggggcgtat tttaacagcc aggaaagcac cgcatcaacc
     6001 tcgcgaaaac gttttcttgc gataacctcg caatcattgc tgaattcaca ttttagcaac
     6061 gtttctattg acctttttgg cgtattgcgc gggagttcag gatctttaaa aatcaccgga
     6121 gtcggaatac ttacaccagg gtgcgccacc agataccact tctctggcgg atccaccggc
     6181 gttagtattt caccaacgcc ttcggcaaac gcggcatgcc cccgaacaaa gacaggaaca
     6241 tctgcgccca gcgtcagccc catttccgcc agctcatcca tgcttagccc gcattgccag
     6301 agatgattta atgccaccag gaccgtcgcg gcattggatg aaccaccgcc gagaccgccg
     6361 cccatcggca aacgcttgtc aatgctgata ttcgcaccgc ttcccgtcgg aagacgcccg
     6421 ctgtctgccg cagttttcat caacaaccgc gctgcgcgaa cgatcaggtt atcttcatgt
     6481 tccacgcctt caacgggcgt taacagacga atatccccat cgtcacgaag ctcaatgctg
     6541 atggtgtcgc cgtaatcaag aaactgaaac agcgtttgca gcgtgtggta accatccgca
     6601 cgctgaccgg taatgtataa aaacagatta agttttgccg gagagggcca ctgtgtccgc
     6661 attatttcac tatccagtta tccattttta acttgatgcg ttgaccaccg tcggtgagtt
     6721 ccatattggt tggcatcgca ggttgcgttt tggtgtcata accaccataa acaaccttcc
     6781 agtttttgcc attctggctg taggtaattt cgctcaggcg gtactggtcg tccagtttgt
     6841 agtcggttgc atcacccggt aaacctaaaa tccactggcg caagctgttg agcggaattg
     6901 gcattccggt caatttgcca atcatctctt cggcgtcatc ggcggtataa cgctgacctt
     6961 tattgtcgac taactgcacg ttacccggtt gagcattcag ctccagttcc gtgctgccca
     7021 atgggttagt gagcagcaga cggtagcgat cctggccggt ttgctgccag aaaaagcggg
     7081 cgtacacttt ttgttggtca gaaatataag cgaacgcgcc gcgagtctga tactgattaa
     7141 gattgcgcac gtcttgctga tgctgacgcc attgtggcga atccgggctt ttgccaggac
     7201 ctttgggcgt ggtaacggaa caggcagtga gcacaagagc agccagcggt agcaggcgga
     7261 taagacgaaa atcgggcagg ggcatagtga tgacaagtcc ttgagatacg ttgcagttat
     7321 aacccttaat gctagcgtta ccgtccgcta tcgtctatgt tcaagttgtc ttaattgcca
     7381 gaatctaacg gctttcggca attactccaa aagggggcgc tctcttttat tgatcttacg
     7441 catcctgtat gatgcaagca gactaaccct atcaacgttg gtattatttc ccgcagacat
     7501 gaccctttta gcactcggta tcaaccataa aacggcacct gtatcgctgc gagaacgtgt
     7561 atcgttttcg ccggataagc tcgatcaggc gcttgacagc ctgcttgcgc agccgatggt
     7621 gcagggcggc gtggtgctgt cgacgtgcaa ccgcacggaa ctttatctta gcgttgaaga
     7681 gcaggataac ctgcaagagg cgttaatccg ctggctttgc gattatcaca atcttaatga
     7741 agaagatctg cgtaaaaacc tctactggca tcaggataac gacgcggtta gccatttaat
     7801 gcgtgttgcc agcggcctgg attcactggt tcttggggag ccgcagatcc tcggtcaggt
     7861 taaaaaagcg tttgccgatt cgcaaaaagg tcatatgaag gccagcgaac tggaacgcat
     7921 gttccagaaa tctttctctg tagcgaaacg cgttcgcact gaaacagata tcggtgccag
     7981 tgctgtgtct gtcgcttttg cggcttgtac gctggcgcgg cagatctttg aatcgctctc
     8041 tacggtcaca gtgttgctgg taggcgcggg cgaaaccatc gagctggtag cgcgtcatct
     8101 gcgcgaacat aaagtacaga agatgattat cgccaaccgc actcgcgaac gtgcccaaat
     8161 tctggcagat gaagttggcg cggaagtgat tgccctgagt gagatcgacg aacgtctgcg
     8221 cgaagccgat atcatcatca gttccaccgc cagcccgtta ccgattatcg ggaaaggcat
     8281 ggtggagcgc gcattaaaaa gtcgtcgtaa ccaaccaatg ctgttggtgg atattgccgt
     8341 tccgcgcgat gttgagccgg aagttggcaa actggcgaat gcttatcttt atagtgtgga
     8401 cgatctgcaa agcatcattt cgcacaacct ggcgcagcgt aaagccgcag cggttgaggc
     8461 ggaaactatt gtcgctcagg aaaccagcga atttatggcg tggctgcgag cacaaagcgc
     8521 cagcgaaacc attcgcgagt atcgcagcca ggcagagcac gttcgcgatg agttaaccgc
     8581 caaagcgtta gcggcccttg agcagggcgg cgacgcgcaa gccattatgc aggatctggc
     8641 atggaaactg actaaccgct tgatccatgc gccaacgaaa tcacttcaac aggccgcccg
     8701 tgacggggat aacgaacgcc tgaatattct gcgcgacagc ctcgggctgg agtagcagta
     8761 catcattttc tttttttaca gggtgcattt acgcctatga agccttctat cgttgccaaa
     8821 ctggaagccc tgcatgaacg ccatgaagaa gttcaggcgt tgctgggtga cgcgcaaact
     8881 atcgccgacc aggaacgttt tcgcgcatta tcacgcgaat atgcgcagtt aagtgatgtt
     8941 tcgcgctgtt ttaccgactg gcaacaggtt caggaagata tcgaaaccgc acagatgatg
     9001 ctcgacgatc ctgaaatgcg tgagatggcg caggatgaac tgcgcgaagc taaagaaaaa
     9061 agcgagcaac tggaacagca attacaggtt ctattactac caaaagatcc tgatgacgaa
     9121 cgtaacgcct tcctcgaagt ccgtgccgga accggcggcg acgaagcagc gctgtttgca
     9181 ggcgatctgt tccgtatgta cagccgttac gccgaagccc gccgctggcg ggtagaaatc
     9241 atgagcgcca gcgagggtga acatggtggt tataaagaga tcatcgccaa aattagcggt
     9301 gatggtgtgt atggtcgtct gaaatttgaa tccggcggtc atcgcgtgca acgtgttcct
     9361 gctacggaat cgcagggtcg tattcatact tctgcttgta ccgttgcggt aatgccagaa
     9421 ctgcctgacg cagaactgcc ggacatcaac ccagcagatt tacgcattga tactttccgc
     9481 tcgtcagggg cgggtggtca gcacgttaac accaccgatt cggcaattcg tattactcac
     9541 ttgccgaccg ggattgttgt tgaatgtcag gacgaacgtt cacaacataa aaacaaagcg
     9601 aaggcgcttt cagtgctcgg tgctcgcatc cacgctgctg aaatggcaaa acgccaacag
     9661 gccgaagcgt ctacccgtcg taacctgctg gggagtggcg atcgcagcga ccgtaaccgt
     9721 acttacaatt tcccgcaggg gcgcgttacc gatcaccgca tcaacctgac gctctaccgc
     9781 ctggatgaag tgatggaagg taagctggat atgctgattg aaccgattat ccaggaacat
     9841 caggccgacc aactggcggc gttgtccgag caggaataat ggaatatcaa cactggttac
     9901 gtgaagcaat aagccaactt caggcgagcg aaagcccgcg gcgtgatgct gaaatcctgc
     9961 tggagcatgt taccggcaga gggcgtactt ttattctcgc ctttggtgaa acgcagctga
    10021 ctgacgaaca atgtcagcaa cttgatgcgc tactgacacg tcgtcgcgat ggtgaaccca
    10081 ttgctcattt aaccggggtg cgagaattct ggtcgttgcc gttatttgtt tctccagcga
    10141 ccttaattcc gcgcccggat acggagtgtc tggtggagca ggcactggcg cggttgcctg
    10201 aacaaccttg ccgtattctc gatctcggga cggggaccgg ggcgattgcg cttgcgctgg
    10261 ctagcgagcg cccggactgc gaaattatcg ctgtagatcg tatgcctgat gctgtctccc
    10321 tggcacaacg taatgcccag catctggcga tcaaaaatat ccacattctg caaagcgact
    10381 ggtttagcgc gctagccggg cagcagtttg cgatgattgt cagcaatccg ccgtatattg
    10441 acgagcagga cccacatctt caacaaggcg atgtccgctt tgagccgctc actgcgctgg
    10501 ttgcggcaga cagtggaatg gcagacatcg tgcatatcat cgaacagtcg cgtaacgcgc
    10561 tggtatccgg cggctttctg cttctggaac atggctggca gcagggcgaa gcggtgcgac
    10621 aagcatttat cctcgcgggg tatcatgacg tcgaaacctg ccgtgactat ggtgataacg
    10681 agcgcgtaac gctcggccgc tattatcaat gacaagtttt tctacactgc ttagtgttca
    10741 tcttattagt atcgcgcttt ctgttgggct attaacctta cgtttctggc tacgttatca
    10801 gaagcatcca caggcatttg ctcgctggac gcgcattgtg ccgccggttg tcgatacggt
    10861 gttattgtta agcggcattg cgttgatggc taaagcgcac atcctgccat tttccgggca
    10921 ggcacagtgg ctgactgaaa agctgtttgg agttatcatt tatatcgttt tgggttttat
    10981 tgcactcgat tatcgtcgta tgcacagtca gcaggcgcgc attattgcct tcccgctggc
    11041 gttggtggtg ctgtacatca tcattaaact cgccaccaca aaagtaccgt tactggggta
    11101 agttatgaga tcgttagctg atttcgaatt taataaagcg ccattgtgcg aaggcatgat
    11161 cctggcttgc gaagcaatcc gccgcgattt tccctcgcaa gatgtttacg acgaactgga
    11221 gcgtctcgtt agtctggcga aggaagaaat cagccagctt ctgcctttag aagagcagct
    11281 ggaaaaactg atcgcgctgt tttacggcga ctggggattt aaagcctcac gcggcgttta
    11341 tcgtctttcc gatgcattat ggctggacca ggtgttaaag aatcgacagg gcagtgcggt
    11401 atcattaggt gcggttttat tatgggtcgc gaatcgtctc gatttgccgc tgctgccggt
    11461 gattttccct acgcagctga tattgcgcat tgaatgtccg gatggcgaaa tttggctgat
    11521 taatcctttt aacggtgaat cgttaagcga acatatgctg gacgtatggt taaagggaaa
    11581 tatcagcccg tcggcggaac tgttttatga agaccttgat gaagctgata acattgaggt
    11641 aatccgcaaa ttgctggata cactcaaagc ctcgttgatg gaagaaaatc agatggagct
    11701 ggcgttacgc accagcgaag ctttattaca attcaaccct gaagatccct atgaaattcg
    11761 cgatcgcggg ttgatttatg cgcaactgga ttgcgaacac gttgcgttga acgatttaag
    11821 ttatttcgtt gaacagtgtc cggaagaccc gatcagcgaa atgatccgtg cgcaaataaa
    11881 taacatcgcg cataaacata ttgtgctgca ttaattaatc gacattttac tcaagattaa
    11941 ggcgatccta tgaaacaaaa agtggttagc attggcgaca tcaacgtagc aaatgacctg
    12001 ccgttcgtac tgtttggcgg tatgaacgtg ctggaatctc gcgatctggc gatgcgcatt
    12061 tgcgagcact acgtaactgt gacccagaaa ctgggtatcc cttacgtgtt caaagcctct
    12121 tttgacaaag ccaaccgctc ctccatccac tcttatcgtg gaccgggcct ggaagaaggg
    12181 atgaaaatct tccaggagtt gaagcagact tttggcgtga aaattatcac cgacgttcac
    12241 gaaccaagtc aggcacagcc cgttgctgat gtcgtggatg tgattcagtt gccggcgttt
    12301 cttgctcgcc agactgacct ggttgaagcc atggcgaaaa ccggtgcggt aattaacgtc
    12361 aagaaaccac agtttgtcag cccggggcag atgggtaata tcgttgataa attcaaagaa
    12421 ggcggcaacg aaaaagtgat tctttgcgat cgtggtgcta acttcggcta tgacaacctg
    12481 gttgtcgata tgctgggctt cagcattatg aagaaagtgt ctggtaactc gccggtgatt
    12541 ttcgacgtga cccacgcact gcaatgccgc gatccgtttg gcgcagcttc cggtggtcgt
    12601 cgtgctcagg tggctgagct ggcacgagcc ggtatggcgg taggtctggc ggggctgttt
    12661 attgaagcgc atccggatcc ggaacatgcg aaatgtgatg gtccatccgc gctgccgctg
    12721 gctaaactgg aaccgttcct caagcagatg aaagcgattg atgatctggt gaaaggtttc
    12781 gaagaactgg ataccagcaa gtaatctttt ttgcttgaaa aataaagtct tagcgttctg
    12841 cgttaagact tttttcatgg gtgccagata caaaaaaggc cgcaggctgt tacccctgcg
    12901 gccggtttcg ggcgcatatt gccatcacgg cagcctgacg cccgttttca ccttacttcc
    12961 ggttacgcca ccagccgaca atcgctgcgg taataattcc cgccaggatc ggtgctgcca
    13021 ggtcgtgcca gaaaatcatg gcaaactgcg cgagcgtcat atagccgcct tgttgtaatg
    13081 acaacatttt gcggctattc ttgaagtgtc tggtttcaag a
//
LOCUS       DAJTXY010000111        12866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTXY010000111 DAJTXY010000000
VERSION     DAJTXY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12866
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.111"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..389)
                     /gene="yafO"
                     /locus_tag="OO566_003226"
     CDS             complement(<1..389)
                     /gene="yafO"
                     /locus_tag="OO566_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6606409.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFR"
     gene            complement(392..685)
                     /gene="yafN"
                     /locus_tag="OO566_003227"
     CDS             complement(392..685)
                     /gene="yafN"
                     /locus_tag="OO566_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR6606410.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(737..1792)
                     /gene="dinB"
                     /locus_tag="OO566_003228"
     CDS             complement(737..1792)
                     /gene="dinB"
                     /locus_tag="OO566_003228"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6606411.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1863..2648)
                     /gene="lafU"
                     /locus_tag="OO566_003229"
     CDS             complement(1863..2648)
                     /gene="lafU"
                     /locus_tag="OO566_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6606412.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2620..4332
                     /locus_tag="OO566_003230"
                     /pseudo
     CDS             2620..4332
                     /locus_tag="OO566_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4549..5046)
                     /locus_tag="OO566_003231"
     CDS             complement(4549..5046)
                     /locus_tag="OO566_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6606413.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTIQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(5222..5743)
                     /locus_tag="OO566_003232"
                     /pseudo
     CDS             complement(5222..5743)
                     /locus_tag="OO566_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            6127..6867
                     /locus_tag="OO566_003233"
     CDS             6127..6867
                     /locus_tag="OO566_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6606414.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6838..7605)
                     /locus_tag="OO566_003234"
     CDS             complement(6838..7605)
                     /locus_tag="OO566_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6606415.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(7811..8389)
                     /gene="lpcA"
                     /locus_tag="OO566_003235"
     CDS             complement(7811..8389)
                     /gene="lpcA"
                     /locus_tag="OO566_003235"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6606416.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            8629..11073
                     /gene="fadE"
                     /locus_tag="OO566_003236"
     CDS             8629..11073
                     /gene="fadE"
                     /locus_tag="OO566_003236"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6606417.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDTMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(11116..11562)
                     /gene="ivy"
                     /locus_tag="OO566_003237"
     CDS             complement(11116..11562)
                     /gene="ivy"
                     /locus_tag="OO566_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6606418.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            11743..12513
                     /gene="yafV"
                     /locus_tag="OO566_003238"
     CDS             11743..12513
                     /gene="yafV"
                     /locus_tag="OO566_003238"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6606419.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            12622..12854
                     /locus_tag="OO566_003239"
                     /pseudo
     CDS             12622..12854
                     /locus_tag="OO566_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         3050 a         3329 c         3411 g         3076 t
ORIGIN      
        1 atgcgaaacg cttctgccat ttttccaatt ttatgcattt ggttgttatc tcgagccagt
       61 ttatgaggtt caggcttcag aatggcaatg agcaaccatg cttgctcatc aaacgcaccc
      121 tgacaatata ccaaatgcgc ttcgtcattc gttctgctga attggcgcaa ctgtggcgga
      181 aatggattat tcacatttgc cagatgaata tgagcaactc gctcaaattt gattaatggc
      241 caggtaaacg agtcgtcgta gagtgcatcg cgaccaaata tatctggcaa aacaccgtca
      301 cgcttatagg aaataaaatc cgccgttaac gcatcaagtt cctctgctgt aagttgcagg
      361 cgaataagtt ttgttttgaa tacccgcatc cttattcctt aaagtcattg aaatcatcat
      421 ccgtcatatc attaagatat tgtttagcgc ggcgtgtaat ttcctctaat cttgcagcac
      481 ttggacgaaa gcgcgccttt atgggctttt tctcctcttg ttcagcaagc atgtccatta
      541 acgcttcgaa tgcgctggca cttaagagat atcctgcggg gcgattatta gaaagaaccg
      601 caactggttg atcaataaag tatttagctg ggtttttacg taactcagtg atattgaccg
      661 atttttcagc gagaatccga tgcatacagt gataccctca taataatgca caccagaata
      721 tacataatag tatacatcat aatcccagca ccagttgtct ttccatttgc gggtcaagca
      781 acgtcacatg cagccccacc agacgcacac cgcgcccgcc gcggcgttca tcccaggttt
      841 tacgcgcggt ggcgattaag tcagctttat tcagccgcgg ccagacgtgc tcctgggtgg
      901 tctgctgaaa atcgtcgaac tttaatttca ccccctggcg agcaatcagt aaatcaggtt
      961 ttacctttgc cagacggcgt tcaagttccg gatacagccg ctcgataatc gcttcacatt
     1021 cagaccagtg atgaatatct tccgccatcg tgcgttccac gccgacggat tttcgtaacc
     1081 gttcgctgtt aacgtcgcgc tcgtcaatcc cctgactacg ctcccacaaa atgcggccaa
     1141 atttgccaaa gcgtttaagc agaatcacca gatcacactt ttgtacatca ccgcaggtcc
     1201 gcagccccat cgcttccagt tttgccgccg agactttgcc gacgccgggg attttttcca
     1261 gtggtaaggt ttgtaaaaat gccggaactt ctgccggcgt aatcacaaac tgaccgttgg
     1321 gtttattcat gtcggaggcg attttggcga gaaactttac tggtgccacg cccgcagatg
     1381 ccgtcagttg cagctcgttg aagatcgtct ggcggatttc ctgggcgatg agggtcgcag
     1441 aaccgtggca atggacgcta tcggtaacat cgagataagc ctcatccagt gacaacggtt
     1501 caatgcgcga ggtgtagcgc gagaagattt cacggatatg atttgaggct tctttgtagg
     1561 cgtcaaagcg ccccggaagc aaggtgagat gtgggcataa tttgagcgcc atcccagtag
     1621 gcatagcgct acgtacgcca aatttacgcg cgggataatt ggcggtgctg atcacccccc
     1681 gacgttcgcg gctgccgcca atagcaatag ggatatcgcg cagagcggga ttgtcgcgca
     1741 tctccaccgc tgcgaaaaag cagtccatat ccacatgaat gattttacgc attgctcacc
     1801 tctcaacact ggtaaagtat acagtgattt caggttttga gaaatgcgta aagattcagc
     1861 atttagcgca ttcgctgcga aagcacctgc tgcttatcca gcttttgcac cagcggctgc
     1921 accactttat ccccatgctg accaaagtat tgatacagcg tatcggacgc acttttggtc
     1981 agtaccataa tctcaatacg ccgattgccc gcgctttgcg gattcttcgc gtccagcagc
     2041 atctggtccg ccattgcgct tacctgcatc actttatctt ccggcattcc ggcctcttcc
     2101 agcacccgac gagctgaaag cgcgcgatcg cccgaaaggt tccagttgtt gtagatattg
     2161 tttttgtagg ccatcgcatc ggtatgcccg gtaataataa ttttgttata gagcgagtcg
     2221 aacactggcg cgagctccac cagcaaagtt ttaaagaacg gcataatctg cgcgctgccg
     2281 cgttcgaaca tattgcgatt ctggtcgtct ttaatcagca cgcgtaatcc ctggggaaca
     2341 atctccatct ccagattcgc ttccatatgc gcatcacggg caatggtgtt gatactggtt
     2401 gccaactcgc caagctcggt ggcggatttc ttcttcagca gcgccgtttt ctcattcaca
     2461 tcacgcgctt ttttctccgt ctcttccggc accgccacgg tggcaggttt tggcggatga
     2521 gacgggctga ttttatttaa cggcgacagc ccaccaccgt taaaaatcga ctgcccgtgc
     2581 agcgcggcga taatgctttc acgctcggat ttactgacgc tgttgacaat caactttatc
     2641 gtcgtcacca aagaggccga gcgtatttcc gaggtttctg cccgctttac cctggacgcg
     2701 atgcccggca aacagatggc gattgacgcc gatcttaacg ccggattgat caaccaggcg
     2761 caggcgcaaa cccggcgtaa agatgttgcc agcgaggccg atttctacgg cgcgatggac
     2821 ggggcatcga agtttgtacg cggagacgcc atcgccggga tgatgattct ggcgatcaac
     2881 ctgagcggcg gcgtctgtat cggcatcttc aaatacaacc tgagcgccga cgccgccttc
     2941 cagcaatacg tgctgatgac catcggcgac ggcctggtgg cgcagatccc ttccctgctg
     3001 ctctccaccg cggcggcgat tatcgtcacc cgcgtcagcg acaacggcga tatcgcccat
     3061 gacgtgcgcc accagttgct ggcaagcccg tcggtgctct acaccgccac cgggatcatg
     3121 ttcgtgctgg cggtggtgcc ggggatgccg catctgccgt ttttgctgtt cagcgccctg
     3181 cttggcttta ccggctggcg gatgagcaaa cgcccgcagg cggcggaggc ggaagagaaa
     3241 agcctcgaaa cgctgacccg caccatcact gaaaccagcg aacaacaggt cagttgggaa
     3301 actattccgc tgatcgagcc catcagctta agcctcggct acaagctggt ggcgctggtg
     3361 gacaaagcgc agggcaaccc gctcacccag cggattcgcg gcgttcggca ggtgatttcc
     3421 gacggtaacg gcgtgctgct gccggagatc cgcattcggg aaaacttccg cctcaagccc
     3481 agccagtacg ctattttcat caacggcatt aaggctgatg aagcggatat tccggcggat
     3541 aaactgatgg cgctgccctc cagcgaaacc tacggcgaga ttgacggcgt gctggggaac
     3601 gacccggcgt acgggatacc ggtcacctgg atccagcctg cgcagaaagc gaaggctctg
     3661 aatatggggt atcaggtgat cgacagcgcc agtgtgattg ccacccatgt gaacaagatt
     3721 gtgcgcagct atattcctga tttgtttaac tatgatgaca tcacgcagtt gcataaccgt
     3781 ttgtcgtcga tggcaccgcg cctggcggag gatttaagcg cggcgctcaa ttacagccaa
     3841 ttgctgaaag tgtaccgggc gctgctgacc gaaggcgttt ccctgcgcga tatcgtcacc
     3901 atcgccaccg tgctggtcgc cagtagcacg gtgactaaag atcatattct gctggcggcc
     3961 gatgtgcgcc tggcgctgcg gcgcagcatt actcatccgt tcgttcgcaa gcaggagctg
     4021 acggtgtata cgctgaataa tgagctggaa aatctgctga ccaacgtagt gaatcaggcg
     4081 caacaggccg gcaaagtgat gctcgacagc gtgccggttg acccgaatat gctcaaccag
     4141 ttccagagca cgatgccaca ggtgaaagag cagatgaaag cggcggggaa agatccggtg
     4201 ctgctggtgc cgccgcagct acgccctttg ctggcgcgtt atgcaaggtt gtttgcgccg
     4261 gggctgcatg tgctgtcgta taacgaagtg ccggatgagc tggagttgaa gattatgggg
     4321 gcgttgatgt aagaatccgg catcggtgca caattgccgg atgcggcgta aacgccttat
     4381 ccggcctacc gttcaggcac aagtttttag gcatgataag acgcgccagc gtcgcatcag
     4441 gcatctgcgc ataactgccg gatgcggcgt gaacgcctta tccggcctac cgttaaagca
     4501 cgatttgtag gccggataag cgtaagcgca tccggcacca gcgcacagtt ataaaatgat
     4561 acgctccccg gcacttaaat ccgttatgtc tcccgcccaa tcgatgggat ataaccctcg
     4621 cgcgacatcg cgatggaacg ttgagaatgg ccaatcactc acctgcttta cccagccatg
     4681 tttctctgga tttatataaa tataatcaac atgatggcga taatcttttg tattgcgaat
     4741 ggcatgctcc caaaaacgcg gttgccagat atttttcaat ccgcaagcat gggtgaactg
     4801 ctttttaatc tcccgccagc gagaggaaaa atcatcatcg ctttcgggta atgtccatat
     4861 gcaatgcata tgctccggca aaacgaccca ggcgttaatt tcaaaaggtc tgtctcgctt
     4921 aacgttaata atggcgttac ggagcgtctg gaactggatg gtcagaagat ggcttcgacg
     4981 attgcgtaaa tttaccgtga aaaaccatgt tccccccttg atgtaataac gacgatattc
     5041 cgacatagcg actccttttt ctcaacggta agaggaaaag gaaaggccga caatgacgct
     5101 gtgttatatc gggaaagcgg ctcgcaaaaa cgcacagatt tgtaggcatg ataagacgcg
     5161 tcagcgtcgc atcaggcatc cgtgcacaac cgccggatgc ggcgtaaacg ccttatccgg
     5221 cctacaaaat catctcttcc gtcaaaatcc tgcgtgcgcc taaaaaatgg tcctgccaga
     5281 aaggttcggc taaccggctt acccgaatgg tttccccggt gcgcagtgac tcaataaact
     5341 gaccgtcgcc cagatacacc cccatatggt cggcaatctc gcggctgtgg atatggaaaa
     5401 atagcaaatc tccccggcgc aggtcgttat tcgccacaat cgttgcccga cggtagtggt
     5461 acatctcatt agccgtgcgc gggagtttcg cctcaaggat cttgttgtag gcataaaaaa
     5521 ctaacccact acaatcgaag ccttgatcgg gccgcgtgcc gccccagacg taaggcttgc
     5581 caagctgctg ctccaggcgg tgaatcgcca cttcggtgat gttatgcagg tgatcgctgc
     5641 ttaaaaagtg gttgttttcc gccagctcct gccagcgtct gtcactcgcc ttgagcggcg
     5701 caggaaacca ttctggatgc tgttttatct gctcccggtt gtggtggcgc tggcgagtaa
     5761 agggagcaaa acgcataacg aaaacacgcc tggatgcaaa aatctgccga gcaagaacga
     5821 agacatgaaa gacataaacc ggctgaatga aggtgagaat tttaaaagac cggaggagta
     5881 aacgggatta ttaattgctt atttgcatat tactattgag atttcataat attaggaaat
     5941 aaatgataca tgcgcttttc ggactattga aaactgcccc tgtgctaaat ttgtaaaccc
     6001 agcgccgaca ggcttgcgta aaactctttt ggtgataatg tgagccttcc aaatccagac
     6061 tattccgatt tgggagccat tttgccgtcc tggcgtgtgt aaccgtttta tcaaggaata
     6121 agcagtatgc gtaaaatcgc attaattctt gcgatgttgt tgatcccgtg cgtttcgttt
     6181 gccggtttgc tgggcagcag tagctcgacc acgccagtga gcaaagagta taagcagcag
     6241 ttgatgggat cccctgtcta catccagatc ttcaaggaag aacgtacgct cgatctttac
     6301 gtcaaaatgg gcgagcaata tcaactgctc gacagttata aaatctgtaa atattccggc
     6361 ggcttagggc caaaacagcg tcagggcgat ttcaaaagcc cggaagggtt ttatagcgtc
     6421 cagcgtaatc agttaaaacc agacagccgt tactacaaag cgattaatat tggattcccc
     6481 aatgcttatg accgtgcgca tggttatgaa gggaaatacc taatgatcca cggcgattgt
     6541 gtatccatcg gctgctacgc aatgaccaat cagggtattg atgagatctt ccagttcgtt
     6601 actggtgcgc tggtgtttgg tcagccgagc gtgcaggtga gtatttaccc attccgcatg
     6661 accgacgcca atatgaagcg ccataaatat tccaacttta aggacttctg ggagcagctg
     6721 aagccgggct acgactactt tgagcaaacc cgtaagccac cgaccgtttc tgtggccaat
     6781 ggccgttacg tggtcagcaa gccgttaagc cacgaagtgg tgcagccaca actggcatca
     6841 aactacacgc tccccgaggc aaaataagcg ccattcgcct ggcataattt tttgccaggt
     6901 ttcattgccc gtcagcggct gtgtcgcaat caccgtgacc acatcattcg gtgtggtctg
     6961 cgagctgaag tcgatttcca catcctgatc cagcaacgtt gccacgccaa acggcgcgcg
     7021 gcgggtgatc cagtgtaaat tagtcgagca atacgccatt acatagcgcc cgtccgaaag
     7081 cagcatgttg aaaacgccct tcgcccgcag ttcatccgcc agtgaggcga tatatttaaa
     7141 tactgccgcc atgttgcccg gcgtgcgcgg gtaacgctgc gttaatttat gcagcagcca
     7201 gcaaaaggct ttttcgctgt cggtttcacc gaccgggcgg aagttgccgg tttccagtga
     7261 tttgtagccc gtcagttgtc cgttatgggc ataagtccag ttacgccccc ataactcgcg
     7321 ggtaaatggg tgagtatttt ccagcgccac ctcgccccga ttagcctggc gaatatgagc
     7381 caccaccgaa caggatttta tcgggtagtc ctggacaagt ttggcgatgg gggaattaaa
     7441 gctgggttgt ggatctttaa atgtgcgaca gcctttacct tcgtaaaagg taatgcccca
     7501 gccatcttta tgtggcccgg ttccaccacc acgctgtaca agtccggtga aactaaagca
     7561 gatatcggta gggacgttgg cgctcatccc gagcaattcg cacatgggta acctccacaa
     7621 cagcgggagc gcatggaccc cggcgaagat tgatttaaga attttcaggt cggatgcggc
     7681 gtaaacgaat tattcggcct acggttcggg tacagatttg taggcatgat aagacgcgtc
     7741 agcatcgcat caggcatcag cgcacaagtg ccggatgcgg cgtaaacgcc ttatccggcc
     7801 tacgccagac ttacttaacc atctcttttt caattaactg gatcaggata tggatcactt
     7861 taatgtgaat ctcctgaatg cggtcggcat aaccaaagtg cggaacgcga atttcgatat
     7921 ccgccgtgcc agccatttta ccgccgtctt taccggtcag ggtgatcact ttcatcccct
     7981 tctcacgcgc cgctgcgatc gctttgatca cgtttgcaga gttaccggag gtggagatcc
     8041 ccagcagtac atcgccttcg cgacctaccg cttcaacgta gcgggagaaa atatcattga
     8101 aaccgaaatc attaccgacg caggaaatat gactaacgtc agaaatagca atcgccgggt
     8161 agcccggacg gttttcacgg tagcgaccgg tcaactcttc ggcaaagtgc atagcgtcgc
     8221 aatgggaacc gccgttgccg caggaaagca ctttgccacc ggctttaaag ctgtctgcta
     8281 acaggaccgc cgcgcgctga atggcgtgaa tattggcgtc atcttttaaa aagttagcca
     8341 gcgtttccgc cgcttcgttc agttcgttac gaataagatc ctggtacatg aggatatcct
     8401 tcagcataaa tgtaatagac aaaatgcagt gtaccggata ccgccaaaag cgagaagtac
     8461 gggcaggtgc tatgaccagg actttttgac ctgaagtgcg gataaaaaca gcaacaatgt
     8521 gagctttgtt gtaattatat tgtaaacaca ttgctaaatg tttttacatc cactacaacc
     8581 atatcatcac aagtggtcag acctcctaca agtaaggggc ttttcgttat gatgattttg
     8641 agtattctcg ctacggttgt cctgctcggc gcgttgttct atcaccgcgt gagcttattt
     8701 atcagcagtc tgattttgct cgcctggaca gccgccctcg gcgttgctgg tctgtggtcg
     8761 gcgtgggtac tggtgcctct gaccattatc ctcgtgccat ttaactttgc gcctatgcgt
     8821 aagtcgatga tttccgcgcc ggtatttcgc ggtttccgta aggtgatgcc gccgatgtcg
     8881 cgcactgaga aagaagcgat tgatgcgggc accacctggt gggagggcga cttgttccag
     8941 ggcaagccgg actggaaaaa gctgcataac tatccgcagc cgcgcctgac cgctgaagag
     9001 caagcgtttc tcgacggccc ggtagaagaa gcctgccgga tggcgaatga tttccagatt
     9061 actcatgagc tggcggatct gccgccggag ttgtgggcgt accttaaaga gcatcgcttc
     9121 ttcgcgatga ttatcaaaaa agagtacggc ggactggagt tctcagctta tgcccagtct
     9181 cgtgtgctgc aaaaactctc tggcgtgagc gggatcctgg cgattaccgt cggcgtgcca
     9241 aactcattag gcccgggcga actgttgcaa cattacggca ctgacgagca gaaagatcac
     9301 tatctgccgc gtctggcgcg tggtcaggag atcccctgct ttgcactgac cagcccggaa
     9361 gcgggttccg atgcgggcgc gattccggac accgggattg tctgcatggg cgaatggcag
     9421 ggccagcagg tgctggggat gcgtctgacc tggaacaaac gctacattac gctggcaccg
     9481 attgcgaccg tgcttgggct ggcgtttaaa ctctccgacc cggaaaaatt actcggcggt
     9541 gcagaagatt taggcattac ctgtgcgctg atcccaacca ccacgccggg cgtggaaatt
     9601 ggtcgtcgcc acttcccgct gaacgtaccg ttccagaacg gaccgacgcg cggtaaagat
     9661 gtcttcgtgc cgatcgatta cataatcggc gggccgaaaa tggccgggca aggctggcgg
     9721 atgctggtgg agtgcctctc ggtaggccgc ggcatcaccc tgccttccaa ctcaaccggc
     9781 ggcgtgaaat cggtagcgct ggcaacgggc gcgtattctc acattcgccg tcagttcaaa
     9841 atctctatcg gtaagatgga agggattgaa gagccgctgg cgcgtattgc cgggaacgct
     9901 tatgtaatgg atgctgcggc atcgctgatt acctacggca ttatgctcgg cgaaaaacct
     9961 gccgtgctgt cggctatcgt taagtatcac tgtacccacc gcgggcagca gtcgattatt
    10021 gatacgatgg atattaccgg cggtaaaggc attatgctcg ggcaaagcaa cttcctggcg
    10081 cgtgcttacc agggcgcacc gattgccatc accgttgaag gggctaacat tctgacccgc
    10141 agcatgatga tcttcggaca aggagcgatt cgttgccatc cgtacgtgct ggaagagatg
    10201 gaagcggcga agaacaatga cgtcaacgcg ttcgataaac tgttgttcaa acatatcggt
    10261 cacgtcggta gcaacaaagt tcgcagcttc tggctgggcc tgacgcgcgg tttaaccagc
    10321 agcacgccaa ccggcgatgc cactaaacgc tactatcagc acctgaaccg cctgagcgcc
    10381 aacctcgccc tgctttctga tgtctcgatg gcactgctgg gcggcagcct gaaacgtcgt
    10441 gagcgtatct cggcacgtct gggggatatt ttaagccagc tttacctcgc ctctgccgtt
    10501 ctgaagcgtt atgacgacga aggccgtaat gaagccgatc tgccgctggt gcactggggc
    10561 gtacaggatg cgctgtatca ggcagaacag gcgatggacg atttactgca aaacttcccg
    10621 aaccgcgtgg ttgccgggct gctgaatgtg gtgatcttcc cgaccggacg tcattatctg
    10681 gcaccgtctg acaagctgga tcataaagtg gcgaagattt tacaagtgcc gaacgccact
    10741 cgttcacgta ttggtcgcgg tcagtacctg acgccaagcg agcataaccc ggttggttta
    10801 ctggaagagg cgttggtgga tgtgattgcc gccgactcga ttcatcagcg gatctgtaaa
    10861 gagctgggta aaaatctgcc atttacccgt ctggatgaac tggcgcacaa cgcgctgacg
    10921 aaggggctga ttgataaaga tgaagccgct attctggtga aagcggaaga aagccgtctg
    10981 cgcagtatta acgttgatga ctttgatccg gaagagctgg cgacgaagcc ggtaaagttg
    11041 ccggagaaag tgcggaaagt tgaagccgcg taattgaata acggagccga aaggctccgt
    11101 ttctttatcc gctaattatt taaaattaaa gccatccgga tggttttcca ggctgccggt
    11161 caacgccgcg aacagcaccg ttttaccatc aatcgaaagc gcatcgttca cattcagcca
    11221 ggtgagtttc tcttgcgacg ttttctcatc aatagccgag aacagccccg tcatctgatt
    11281 agatttctcg gaccacatca cagcgatacg ttgcgagcca cagtcatgcg gtttgcacgc
    11341 gctcatcacc tgatacgtct catctcccaa cgttacggtt tgtgcgggag tataagtacc
    11401 gcctttcatc acccatgcag gtagcttatg cccttgcacc atctgattaa acgcagcttt
    11461 ggtggtttcg ccctttgcaa ggctgctaat ggttaaatca tcctgcgcca ttgcactggt
    11521 ggcgatgacc agtgcggcga ctgtcgttat tgccttaaac atcattcctc ccgagcttat
    11581 cctgcccatg ttattaacct cctaaagcct ggcgataaca cgataatttg caaatcagat
    11641 atttccaata tcagcatttg ttatcaattt attctgcgct aaggcaactg cctgataagg
    11701 tgttaaagtt ataaaaccct tcgcataaag gagttgtaca ccgtgcctag tttgaagatt
    11761 acgcttttgc agcaaccgtt ggtgtgggtg gatggtcctg ccaacctgcg ccattttgat
    11821 cgtcaactgg aaggtattac cgggcgcgat gtgatcgttc taccggagat gtttaccagc
    11881 ggctttgcca tggaagcggc agcttcgtcg cttgcacaag atgacgtagt gaactggatg
    11941 acagctaagg cgcagcagtg caatgcgctg attgcaggca gtgttgcatt acaaacggag
    12001 tctggttcgg ttaaccgctt tttgctggtt gagccgggcg gcacggtaca tttttatgat
    12061 aagcgtcatc tgttccgcat ggcagatgag catctgcatt ataaagcggg caatgcgcga
    12121 gtgattgtgg aatggcgcgg ctggcgtatt ttgccgctgg tgtgctacga cttacgtttt
    12181 ccagtgtggt cgcgcaatct caacgattat gacctcgccc tgtacgtcgc caactggcca
    12241 gctccgcgct ctctgcactg gcaggcattg ctgacggccc gcgcgattga gaatcaggcg
    12301 tatgtggcgg gatgcaatcg cgtcggcagc gatggcaacg gctgccatta tcgcggtgac
    12361 agccgggtga ttaatccgca aggcgagatt atcgctaccg ccgacgcgca taaggcaacg
    12421 cgtattgatg cggagctgtc gatggtggca ttgcgggaat atagagaaaa gtttccggca
    12481 tggcgggatg cggatgagtt taggttgtgg tgagtggggg ttgtgtttag ggccggggct
    12541 gggctagatt acgaaagccc ctaacttaaa cacaatatta cgccagaagt caccatttcc
    12601 aaaacacagc gtcgctgtac cgtgggttat ctgcccaacc gcagcgacac gccggtgatc
    12661 aaaatcagcg ccaaatggct gagaaaagcg ggatttgaga tcggcacggg cgttaccgtg
    12721 aagatctcgg agggatgttt gatcctgctt gggataacaa cgaagtacag gaactgcgca
    12781 gggaacttta tcaggttaaa cagtcagtta aggggatgcg agacgggatg tttagtgtgc
    12841 tgaacgaaag ttaaataata aaaagc
//
LOCUS       DAJTXY010000112        12749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTXY010000112 DAJTXY010000000
VERSION     DAJTXY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.112"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(101..739)
                     /gene="rutR"
                     /locus_tag="OO566_003240"
     CDS             complement(101..739)
                     /gene="rutR"
                     /locus_tag="OO566_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6606420.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1027..2118
                     /gene="rutA"
                     /locus_tag="OO566_003241"
     CDS             1027..2118
                     /gene="rutA"
                     /locus_tag="OO566_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6606421.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2118..2810
                     /gene="rutB"
                     /locus_tag="OO566_003242"
     CDS             2118..2810
                     /gene="rutB"
                     /locus_tag="OO566_003242"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6606422.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGDYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIHHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DTLSPTSFARIA"
     gene            2822..3208
                     /gene="rutC"
                     /locus_tag="OO566_003243"
     CDS             2822..3208
                     /gene="rutC"
                     /locus_tag="OO566_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6606423.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGLVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3216..4016
                     /gene="rutD"
                     /locus_tag="OO566_003244"
     CDS             3216..4016
                     /gene="rutD"
                     /locus_tag="OO566_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6606424.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAVRAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4026..4616
                     /locus_tag="OO566_003245"
     CDS             4026..4616
                     /locus_tag="OO566_003245"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6606425.1"
                     /translation="MNEAVSPGVLSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4627..5121
                     /gene="rutF"
                     /locus_tag="OO566_003246"
     CDS             4627..5121
                     /gene="rutF"
                     /locus_tag="OO566_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6606426.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5142..6470
                     /gene="rutG"
                     /locus_tag="OO566_003247"
     CDS             5142..6470
                     /gene="rutG"
                     /locus_tag="OO566_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6606427.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6552..6659)
                     /locus_tag="OO566_003248"
     CDS             complement(6552..6659)
                     /locus_tag="OO566_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606428.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7032..7628
                     /gene="wrbA"
                     /locus_tag="OO566_003249"
     CDS             7032..7628
                     /gene="wrbA"
                     /locus_tag="OO566_003249"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6606429.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7649..7876
                     /locus_tag="OO566_003250"
     CDS             7649..7876
                     /locus_tag="OO566_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6606430.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7914..9155)
                     /gene="agp"
                     /locus_tag="OO566_003251"
     CDS             complement(7914..9155)
                     /gene="agp"
                     /locus_tag="OO566_003251"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6606431.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9448..10704)
                     /locus_tag="OO566_003252"
     CDS             complement(9448..10704)
                     /locus_tag="OO566_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR6606432.1"
                     /translation="MGSNIHGMSCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10965..11885
                     /gene="cbpA"
                     /locus_tag="OO566_003253"
     CDS             10965..11885
                     /gene="cbpA"
                     /locus_tag="OO566_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6606433.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11885..12190
                     /gene="cbpM"
                     /locus_tag="OO566_003254"
     CDS             11885..12190
                     /gene="cbpM"
                     /locus_tag="OO566_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6606434.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12283..12699)
                     /gene="torD"
                     /locus_tag="OO566_003255"
                     /pseudo
     CDS             complement(12283..12699)
                     /gene="torD"
                     /locus_tag="OO566_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
BASE COUNT         3047 a         3244 c         3211 g         3247 t
ORIGIN      
        1 ttcgctgagt gtgaataccg ctgccgctgg cggtaacgcc agtttaatga cgatcggtta
       61 aacaggtagc cggaggatgt tacaacctcc tccggcatct ttaacgtggt cgaatcccct
      121 caataataat ccgctgcacg ttttcaaccg tttgattgaa aaatacctca tcgcgcaacg
      181 tcgcgcctgt caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc
      241 aaatcataaa aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac
      301 cggcaatcag cgccgatttc tcatcaatta atgccttcaa atcgcccgtc agttcatcca
      361 ttaacaacgg cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat
      421 cgcgtgagac ttccagcttc agacggatgt actcttttat cgccgccagc ggggcgaaat
      481 cttcacgaaa cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa
      541 tatacagcgc ctcttttgac gggaagtaat acagcagatt ggtttttgaa acacccgcca
      601 actctgcgat ctgctccagc cttgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg
      661 ctgcgctaag aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt
      721 tcactgcgcc ttgcgtcatg cgctctcccc tcttgtagat tggtaacagg atagcaaaac
      781 ccccgcccgg cctcgacctt ctgcactctc atcgcgcacg gatgcatgtt ttatgtgcaa
      841 ctgttttgac cgtttagtcc actttttacc agatatttta gataacacaa tgctaacaca
      901 ttaataacat tcacttttta aaaactggca tccgctttgc aaacaagcca atacgcggct
      961 gaaaaggata tgcaggatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg
     1021 ttgatgatga aaattggcgt attcgtacct attggcaaca acggctggct catttcgacc
     1081 cacgcgccgc agtacatgcc gacctttgaa ctgaataaag ccattgtgca aaaagcggag
     1141 cattaccatt tcgatttcgc cctgtcgatg atcaaactgc gtggctttgg cggcaaaact
     1201 gagttctggg atcacaacct tgagtcgttc accttgatgg cgggtctggc agccgtgacc
     1261 tcgcgcattc agatttacgc cactgctgcc accttaacgt tacctccggc aatcgtcgcc
     1321 cgtatggccg caaccatcga ctccatctct ggcgggcgtt ttggcgtcaa cctcgtgact
     1381 ggctggcaaa agcccgagta tgagcagatg ggtatctggc ctggcgatga ctatttctcc
     1441 cgtcgttacg actatctcac cgaatatgtt caggtgctgc gcgacctgtg gggcacgggg
     1501 aaaagcgatt ttaaaggcga ttttttcacc atgaatgatt gtcgcgtcag tccgcaaccg
     1561 agtatcccca tgaaagtgat ctgcgccggg caaagcgacg ctggcatggc gttctccgcc
     1621 cagtatgccg atttcaactt ctgtttcggc aaaggcgtaa atacacccac ggctttcgcc
     1681 ccgaccgctg cgcggatgaa acaggccgca gagcaaaccg gacgcgacgt tggctcttat
     1741 gtgttgttta tggtgattgc cgatgaaacc gacgatgcca ctcgcgccaa atgggaacac
     1801 tacaaagcgg gcgcggatga agaggcgtta agctggctaa ccgaacaaag tcagaaagat
     1861 acccgctccg gtactgacac caacgttcgt cagatggccg atcccacttc ggcggtaaac
     1921 atcaatatgg ggacgttagt cggttcttac gccagtgtcg cgcgcatgtt agatgaagtc
     1981 gcaagcgtgc ctggtgcaga aggcgtgctg ttgaccttcg atgattttct gtcgggaatc
     2041 gaaaccttcg gcgagcgcat tcaaccactg atgcagtgcc gcgcccatct ctctgcgctg
     2101 actcaggagg tggcatgatg acaaccttaa ccgctcgacc ggaagccatt accttcgatc
     2161 cgcagcaaag tgcgcagatc gtggtggata tgcaaaacgc ttatgccacg ccaggcgact
     2221 acttagatct cgccgggttt gatgtctcaa ccactcgccc ggtcattgcc aacattcaaa
     2281 ccgctgtgac cgcagcgcga gcggcaggga tgttgatcat ctggtttcaa aatggctggg
     2341 atgaacagta tgtcgaggct ggcggccccg gctcaccgaa ttttcataaa tcgaacgccc
     2401 tgaaaaccat gcgtaagcag ccgcagctgc aggggaaatt gctggcgaaa ggctcctggg
     2461 attatcaact ggtggatgaa ctggtgccgc agcctggcga tattgtgctg ccgaagccgc
     2521 gctacagcgg tttcttcaat acgccgctgg acagcatttt gcgcagccgt ggaatacacc
     2581 atctggtttt caccggtatc gctaccaacg tctgcgtcga atcgacgcta cgcgatggct
     2641 tttttctgga gcatttcggc gtggtgctgg aagacgcaac acaccaggcg gggccggaat
     2701 ttgcacagaa agccgcgttg ttcaatatcg aaaccttttt tggctgggtc agcgacgtcg
     2761 aaacgttctg cgacacgctt tctcccacgt cctttgctcg tatcgcttaa ggagtttaac
     2821 gatgccaaaa tccgtaatta ttcccgctgg cagcagcgca ccgctggccc ccttcgttcc
     2881 cggcacgctg gctgatggct tggtgtatgt ctccggtacg ctggcttttg atcaacataa
     2941 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
     3001 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
     3061 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
     3121 taaaccggcg cgattctaca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
     3181 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
     3241 cttatgctga tgcacccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
     3301 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
     3361 gcaccggcaa taatcccgac acactggcag aagattacag tatcgcccag atggcagcgg
     3421 aactgcatca ggcgctggta gccgcaggga ttgagcgtta cgcggtggtc ggccatgcgc
     3481 tcggtgcgct ggtggggatg cagctggcgc tggattatcc cgcgtcggta actatgctgg
     3541 tcagcgttaa cggctggcta cgaataaacg cccatacgcg ccgctgtttt caggttcgcg
     3601 aacgattact gtatagcggc ggcgcgcaag catgggtgga agcgcagccg ttgttcctct
     3661 atcccgccga ctggatggcg gtccgcgcac ctcgcctgga ggcagaagac gcgctggcac
     3721 tggcgcattt tcagggcaaa aataatttac tacgtcgact taacgccctc aaacgcgctg
     3781 actttagtca ccatgcggat cgcatccgct gcccggtgca aatcatctgc gccagtgatg
     3841 atctgctggt gccaacagca tgttccagtg aacttcatgc cgccctgccc gatagccaga
     3901 aaatggtgat gccctatggc ggacacgcct gcaacgtgac cgatcccgaa acgtttaatg
     3961 ctctgttact caacgggctt gccagcctgt tacatcaccg tgaagccgcc ctgtaaggaa
     4021 ttgctatgaa cgaagccgtt agcccaggtg tgcttagcac cctgttcacc gatgcccgca
     4081 ctcacaacgg ctggcgggag acacccgtca gcgatgagac gttacaggag ctttatgccc
     4141 tgatgaaatg ggggccgaca tcagctaact gttctccggc acggatcgtg tttatccgca
     4201 ctgcagaagg aaaagaacgt ctgcgcccgg cgctttccag cggcaatctg caaaaaaccc
     4261 tgaccgcgcc cgtcaccgct atcgtcgctt gggacagtga attttatgaa cggttaccac
     4321 tactgtttcc ccacggtgat gcccgcagtt ggtttacctc cagcccacaa cttgccgaag
     4381 aaacagcgtt tcgcaacagt tccatgcagg cggcctatct gatcgtcgct tgccgtgcgc
     4441 tgggactgga taccggcccg atgtcgggtt ttgaccgtca acacgttgac gacgcctttt
     4501 ttgcgggcag cacgctgaag agcaatctgc tgattaatat cggctatggc gatagcagca
     4561 aactttttgc gcgcctgcca cgtctgtcct ttgaagaagc ctgcgggctg ttgtaaggag
     4621 caaactatga acatagttga tcaacaaacc tttcgcgatg cgatgtcctg tatgggcgcg
     4681 gcggtcaata tcatcaccac ggacggtcca gccgggcgcg ccgggttcac cgccagcgcc
     4741 gtctgcagcg tgaccgacac gccgcccaca ttactggtgt gcctgaatcg tggggcgtcc
     4801 gtctggccgg tattcaatga aaatcgaacg ctgtgtgtaa atacactgag cgccgggcag
     4861 gagccgcttt caaatctttt tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc
     4921 cgctggcaga cgggtgtgac cggatgcccg caactggaag aggctctggt ttcgtttgac
     4981 tgccgtatca gccaggtggt gagcgtcggc acccacgaca ttctgttttg cgccatcgaa
     5041 gcgattcatc gtcacaccac accctacggg ctggtgtggt tcgatcgcag ttatcacgcg
     5101 ctgatgcgcc ctgcttgtta acctccttaa ggagacagct gatggcaatg ttcggttttc
     5161 ctcactggca gttaaaatcg acctctacag agagcggcgt ggtcgcgccg gatgaacgat
     5221 tgccgtttgc tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga
     5281 cggtgttaat gccgattctg atggggctgg atcccaatct ttccatttta atgtcgggga
     5341 tcggcacgtt actgtttttc tttattaccg gcgggcgcgt ccccagttat ctcggctcca
     5401 gcgcggcttt tgtcggcgtg gtgattgccg cgacgggctt taacggtcag ggcattaacc
     5461 cgaatatcag catcgcgctg ggcgggatta tcgcctgtgg gctggtttat accgtcattg
     5521 gcctggtggt gatgaaaatt ggtacacgct ggattgaacg actaatgccg ccggtggtga
     5581 cgggtgcggt ggtgatggcg attggcctga acttagcgcc tatcgcagtg aaaagtgtat
     5641 cggcttcggc ctttgatagc tggatggcgg taatgacggt gctgtgtatt gggctggtgg
     5701 cggtgtttac ccgtggcatg atccagcggt tattgattct cgtaggatta attgtcgcct
     5761 gtctgctgta tggcgtgatg accaacgttt tagggctggg aaaagccgtc gattttactc
     5821 tcgtcagcta tgctgcctgg ttcggtctgc cgcatttttc cactcctggt tttaatggcc
     5881 aggcgatgat gttgattgcg cccgtggcgg tgattctggt ggcggaaaac ttaggtcatc
     5941 tcaaggcggt cgccgggatg accggacgca atatggaccc gtatatgggg cgagcattcg
     6001 tcggtgatgg tctggcaacg atgctttccg gctctgtcgg cggtagcggc gtcaccacct
     6061 atgctgaaaa tatcggtgtg atggccgtga caaaagtcta ctcgacgctg gtgtttgttg
     6121 cagctgccgt catcgctatg ctgttgggct tttcgccgaa atttggcgcg ctgatccata
     6181 ccattccggc cgcggtgatt ggtggtgcat caattgttgt gttcgggctg attgccgtcg
     6241 caggcgcaag gatatgggta caaaaccgtg tcgatttaag ccagaacggt aatttgatta
     6301 tggtcgcagt aaccttcgtt ctgggcgcgg gtgattttgc tctcacgctt ggcggtttta
     6361 cgttgggagg gatcggtaca gcaacctttg gcgcgatttt actcaatgcg ttattaagca
     6421 gaaggttggt tgacgttcca ccgccagaag ttgttcatca ggagccataa acagaaaacc
     6481 gccgggttac cggcggtgat gactttttga cgagcttaag aaaaaaatgc attcgtcatt
     6541 gattagcccg gctagttgtc gcttttgccg tgactgctct tgccaccttt tttacctgct
     6601 tctgatgctc tttcgcggtc ttctgcaaaa ttgccggaac cgcctcgatg gtttgccatt
     6661 actgacctcc gtctgggtat agacatcata ttgcatagaa ttaacgcggc gtatggcggc
     6721 tgccatattt tcgctacgtg ggactaagct tagtgaactg acgcgggcag acccgcgttt
     6781 ggtaatatcc tgcaacagct tcttagcgag aatagatatc atctccaata attatccata
     6841 agccgctctt atgattaaaa aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat
     6901 tacgtgcttg tgaaagttgt tataaatcaa ataagtggtt gtgaaatttg cactctgaaa
     6961 aggacgtctt atctttaaat aagtggtagc gaatcgctac ggaatagaga taacacgagg
     7021 agtggttaga aatggctaaa gttctggtgc tttattattc catgtacgga catattgaaa
     7081 cgatggcacg cgcagtcgct gagggtgcaa gcaaagtgga tggtgcagaa gttgtcgtta
     7141 agcgtgtacc ggaaaccatg ccgccgcaat tatttgaaaa agcaggcggt aaaacgcaaa
     7201 ctgcaccggt tgcaaccccg caagaactgg ccgattacga cgccattatt tttggtacac
     7261 ctacccgctt tggcaacatg tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc
     7321 tgtgggcttc cggcgcacta tacggaaaac tggcgagcgt ctttagttcc accggtactg
     7381 gcggcggtca ggaacaaacc atcacatcca cctggacgac ccttgcgcat cacggcatgg
     7441 tgattgtccc cattggctac gcagcgcagg aattatttga cgtttcacag gttcgcggcg
     7501 gtacgccgta cggcgcaacc accatcgcag gcggtgacgg ctcacgccag ccaagccagg
     7561 aagaactgtc tattgctcgt tatcaagggg aatatgtcgc aggtctggca gttaaactta
     7621 acggctaatc ttcaacagga ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt
     7681 cggtgaatgg gcatctctgc gcaatacgtc gccggaaata gccgaggcca ttttcgaagt
     7741 cgccgggtac gacgaaaaaa tggcagaaaa gatttgggaa gaaggtagtg atgaagtgtt
     7801 agtcaaagcc tttgctaaga ccgataaaga ttcgcttttt tggggcgaac agaccatcga
     7861 acgtaaaaac gtttaaccag cgactccccc gcttctcgcg ggggagtttt ctgttatttc
     7921 accgcctcat tcaacacgct atcaaactta tccatcgggc agaaaccgtt ggcatctatc
     7981 gggcaaccgc ttaattccag cgtcacgcgc tgcgcaggtg cctgcagggt taacgcatcg
     8041 gcattacgta actgttccgc actctggtac acatattcaa ttttcatcaa atcgcgattg
     8101 gctttgctgt catgccaacg ctggaaaacg attttgccgc caatcggcgt gcgttcgttc
     8161 tggtcatgca actgatacgg tttgaaatcc agcgccgtta acagagaggc tatgttggag
     8221 tcgtgcccaa ctaacaccgt gattttcgga gcactggcgc gatcggtgac cagggctttg
     8281 tcgatataac tgaccagcgg tttcgcaaca ttgcgcgcca cttccggtga ggtaaacaga
     8341 ctgtcctggt aaccgttttt cagcttcgac aataccttcc actgctggtc agatttgatt
     8401 tctccccagg ccacctgatc catcggaaaa ccttcgtagt attgcaaagt aaacgcatct
     8461 accagcgagt tgccgacttt cagcggcccg gaaacacctg gttcttgttg atacttggcg
     8521 ctaaaggtat ttttgccatc caccagcgaa cactgttgtt tctctttaca ggcaggggaa
     8581 tctttatagt taacgatttt ttccagtagc tggtagctgt cggtaagctg gagtttgctg
     8641 agctctttct ccattgccgc caccgcctgt tcactgaatg cggcggaatc atcggtgatc
     8701 atcgggttaa aggttgggtc catagtgccc attttttcct ggtgatgcac aggaatatca
     8761 cacccaggga atgcaccagt aataaagaac tgggcggtag cgacggtacg ttgcagacta
     8821 ttggcatagg cataaacggt gtccggcggc gggcattccc ccgatttcac tatcccctgc
     8881 tctgccagcc attcacgcat gtaatggccc atatacactt cgagcacgcc acctttggtg
     8941 gtgagttgcc caccggggac gtcccattcg ggccatttat tcggcgtcga ctgctccagc
     9001 acactgccat tgttcgccag cggcgcacgt aagttatggc ggctcatcat gagcacttgc
     9061 tgtagctgat agccttccgg tacggtttgt gcctgagcat ttgaagcgag taaaactatc
     9121 cctgccacag ttgcggcgat tagcgttttg ttcattcctg gcacctcttt tgttatcaat
     9181 cattaaagag tgtgacagaa atatgacttt tttccgggaa caggttcgca aaatggcttg
     9241 ttgaacgcct gtttttctat atacatcttc aaaaacaggc aaggtttgac gcaaactatg
     9301 cctgattagc ccctgcctcg cgtacgccgc tgataattgc acctgtggct aaacagaata
     9361 tctgcggcgt taccaataca gagacaatat ttaccaataa aaaagtgtga gactaataac
     9421 aatggttttt agcatatgcc tttgccatta agttgcataa cgatttaaat agtcaagttg
     9481 cgcattcatt tccttatttt ttaacttata agcctgagaa aaaagttcag gagggaggta
     9541 aacatcataa tgattgccag tgtttattaa ataaccatac tcaagatctt catctgttga
     9601 aaaggattca tttcccaagg agattgatgc cacattacta ttaacaataa ataatgagca
     9661 aatgctatct gaaagaacag caagaaaatt cttcaccgct ttaatcctat ccgacgtgtc
     9721 ggtatctaaa gcaagcaaat tcttgttatc attaaatata aactcgggta aatgcagtat
     9781 gcattttttg tcattcaagc aactatcaat gaattgtttt ttaaaaaaca gaaagctcca
     9841 tgacataaaa ctacccacac cctgaatttt gttgtcagat tgcagatcaa cgccaaataa
     9901 tgtctttaca tcttccctga gattatagaa tgccgtatca acaagagcct cgatcgtttg
     9961 tttatcaaca attttaataa actcttcgta tacttgcttt tgcgtcttat ggctgcccag
    10021 cgcccatgaa tactcatgtt cttttaaaac cagtatggct cttaacagac aatatccatc
    10081 gccatctact ggagcgtatt ttaatccata tgttttatta atttcactct gaataaaaga
    10141 tatgccagac aaatcaatac cagtatctaa atcagatatt ctcggcatat ttatctgttc
    10201 tggctggata ctcatatttt catcatctaa acaatactca gaaaaaacat catcacacgc
    10261 attaatgttt tcgatatatt ccataaagcc ataccaataa taacctttaa aatctgtgat
    10321 agactctgaa cctctgcccg gcaaggacac tctaacaata tcaacatctt gaacatattg
    10381 tatagtatat gtaatacctc ctgaaacccc tgaataaatt tgtgtatcat tagtacgcaa
    10441 tgttctcata aataatgata gatcattatc attctgcatt tcctcaaatt ctttttctag
    10501 tgattcacat tccagacatg gagtatcctc ttcatttact ttacgtctga gttcattgta
    10561 caaccgcata aaacacacca gtgtgttttc aaaaagcgtg attgaatatg tttgattttt
    10621 ttcgtactca ttttttatat cgttccaggc ctgtttcaaa taattatttg cagtgcaact
    10681 cattccatgt atattactac ccatatatag cgtctataaa atttaataaa taatgacgcc
    10741 ctagttaaac ttaaagtgcc tggttcaact atcaaaaatc gctcaccctt tttcacctgt
    10801 ttaaaatatg ttcagcaacc catcttgatg gcgacctcct ctccgcgatg atttcaataa
    10861 catattctgt gttggcatat gaaattttga ggattaccct acacttatag gagttacctt
    10921 acaggggttc cttcaatttg tgttgattta cgcgagataa cgctatggaa ttaaaggatt
    10981 attacgccat catgggcgtg aaaccgacgg acgatctcaa gacaatcaag accgcctatc
    11041 gtcgactggc ccgtaaatac catcctgatg tcagcaaaga accggatgcc gaagcccgct
    11101 tcaaagaggt cgctgaagcc tgggaagtgt taagtgatga acaacgtcgc gctgagtatg
    11161 atcagatgtg gcaacatcgc aacgatccgc aatttaaccg tcagttccat catggcgacg
    11221 gtcagagttt taatgccgaa gattttgacg atatcttctc gtcaattttc ggtcagcatg
    11281 cccgccagag ccgtcaacgc cccgccgcac gcggccacga tattgaaatc gaagtggcgg
    11341 tattcttcga agaaacgctt actgagcata agcgtaccat cagctataac ctgccggttt
    11401 ataacgcctt tggcatgatc gaacaggaaa tcccgaaaac gctgaatgtg aagatcccgg
    11461 caggcgtcgg caatggtcaa cgcatccgcc tgaaaggcca ggggacgccg ggcgaaaacg
    11521 gcggtccaaa tggcgatttg tggctggtga ttcatattgc gccacatccg ctgttcgata
    11581 ttgtcggcca ggatctggaa attgtggtgc cggttagccc gtgggaagcg gcgctgggta
    11641 ctaaagtcac cgttccaaca ctgaaagaaa gcattttgct gactatcccg ccaggcagcc
    11701 aggccgggca acgattgcgc gttaaaggca aaggtctggt gagcaaaaaa cagaccggcg
    11761 atctgtatgc ggtactgaaa atcgtgatgc cgccgaaacc ggatgaaaac actgccgcgc
    11821 tgtggcagca actggcagac gcccagtcgt cttttgatcc acgtaaagat tgggggaaag
    11881 cataatggct aatgttacgg tgacttttac tattaccgaa ttttgcctgc ataccggcat
    11941 ctctgaagag gagttgaatg aaattgtcgg tttgggggtg gttgaaccgc gtgagatcca
    12001 ggaaacaacc tgggtatttg acgaccatgc cgccattgtg gtgcaacgcg cggtacgcct
    12061 gcgtcatgaa ctggctctgg actggccggg gatcgcggtg gcgctgacgt taatggatga
    12121 tattgcgcac ctgaagcagg aaaaccgcct gctacgccag cggctttccc ggtttgtagc
    12181 tcatccgtga tgtgtgtttg ccggatgtga tgacgcatcc ggcaactgcg ccataatgcc
    12241 tgatgcgacg ctaaacgcgt cttttcaggc gcataaacga cgttatctgt tttggtggtc
    12301 gcactccact aacaccagca ataactggct tagtgccgcg taaaaaccaa agctgtcata
    12361 ctgtcgacaa cgcgcagcaa actctggtaa ccattgccgt agcgccgtca gtattttttg
    12421 ccgcaaactg tcgattcttc gcgcaggaac ggtcccctct cccagcgaaa aatgcagata
    12481 actcaacaac tcgagataga tcgccagatg atctgccggt tcgttgaaat tgccactggt
    12541 ttccatccct gcctcaacta acaagcgttt aatctcttgc tcgtcctgtt tgtaggccga
    12601 tgcatacggc agcgccgctt gtttgtcggt catcagaaac aggccgcaaa agtcagcggc
    12661 cagttccaga cgggcatcgt cacgtactgt cagcgtggca ggtaatgctg ccaacttact
    12721 gatttagtgt atgatggtga ttttaaggt
//
LOCUS       DAJTXY010000113        12270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTXY010000113 DAJTXY010000000
VERSION     DAJTXY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.113"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..490
                     /locus_tag="OO566_003256"
     CDS             <1..490
                     /locus_tag="OO566_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6606435.1"
                     /translation="VSVATRQVAGNTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTA
                     RIRVNRGNLPAIKLNTTRRRAGEGLRVGKYFFRGAFVQQLANGRWHVLRCLPEARFAT
                     GHDHQGRLRKNRLPVEVVKIPMSGPLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHL
                     SK"
     gene            487..886
                     /locus_tag="OO566_003257"
                     /pseudo
     CDS             487..886
                     /locus_tag="OO566_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            897..1637
                     /locus_tag="OO566_003258"
     CDS             897..1637
                     /locus_tag="OO566_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6606436.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1696..2082
                     /locus_tag="OO566_003259"
     CDS             1696..2082
                     /locus_tag="OO566_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6606437.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2091..2420
                     /locus_tag="OO566_003260"
     CDS             2091..2420
                     /locus_tag="OO566_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6606438.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2392..5331
                     /locus_tag="OO566_003261"
                     /pseudo
     CDS             2392..5331
                     /locus_tag="OO566_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5331..5660
                     /locus_tag="OO566_003262"
     CDS             5331..5660
                     /locus_tag="OO566_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6606439.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5660..6358
                     /locus_tag="OO566_003263"
     CDS             5660..6358
                     /locus_tag="OO566_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6606440.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYDRFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6363..7106
                     /locus_tag="OO566_003264"
     CDS             6363..7106
                     /locus_tag="OO566_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6606441.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            7043..7644
                     /locus_tag="OO566_003265"
                     /pseudo
     CDS             7043..7644
                     /locus_tag="OO566_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            7705..11184
                     /locus_tag="OO566_003266"
     CDS             7705..11184
                     /locus_tag="OO566_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6606442.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLERWDTPKVVKGVSFLLRLTVAADDGSERLVSTGRTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDYAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKTLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTTNKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            11252..11851
                     /locus_tag="OO566_003267"
     CDS             11252..11851
                     /locus_tag="OO566_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6606443.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11915..>12270
                     /locus_tag="OO566_003268"
     CDS             11915..>12270
                     /locus_tag="OO566_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6606444.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPWYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGA"
BASE COUNT         2874 a         2909 c         3922 g         2565 t
ORIGIN      
        1 ggtctcggtt gccacccggc aggttgccgg gaataccgtt gcgggcgata accaggtgaa
       61 agggatcccc ctgaaactgg tacgtcagcg tgtccgggtg tttaaagcca gtccgtcagg
      121 aaaaatgacg gccaggatcc gcgttaaccg gggcaatctg cccgccatca aactgaacac
      181 aacacggcgg cgtgctggtg aaggactgag agtgggaaaa tactttttcc ggggggcatt
      241 tgttcagcaa ctggcgaatg gccgctggca tgttctgagg tgtcttcctg aagcgcgttt
      301 tgcaacaggg catgaccatc agggcaggct aagaaaaaat cgtcttcctg tggaggtagt
      361 gaaaatcccg atgtccggac cgctgacaca ggcatttgaa gatgcccgcg atcgcatcat
      421 tgctgcggaa atgccgaaac agctggggta tgccctgaaa caacaactga ggttacatct
      481 gagtaaatga accggcacac acaaatccgt cagagcgtgc tggcacgcct tcgggaacag
      541 tgtggagaca gcgccacgtt ttttgacggg cttccggcat ttattgatgc gcaaactgcc
      601 tgccgtggcg gtgtggctga gtgatgctca gtacaccgga aaaatgacgg atggagatga
      661 ctggcaggct gttctgtata ttgccgtctt catccgggca caggcaccgg attcagagct
      721 ggatatgtgg atggagagca ccattttccc ggccctgaat gatgtaccgg cactttccgg
      781 actcatcgac accctgaacc cactcggttt taactatcaa cgtgataatg agatggctac
      841 ctgggcgatg gcggaaatca cgtaccagat cacgtacacg aattaaggag gtggtaatga
      901 ccacaccaaa tccactggca aaaacgaaag gtgcgggaac gacgttatgg atgtataccg
      961 gcaacggcga cgcatttgcg aaccctttgt cggacactga ctggctgcgt cttgcgatgg
     1021 tgaaggatct gcaacctggc gagatgaccg ctgatgcaga agatgacact tatctcgatg
     1081 atgaagatgc agactggaaa acgacaaccc aggggcagaa atccgtcggt gatacttcgg
     1141 cgacgctggc ctggcgtccg ggtgacagcg ggcagaaaaa actggttcag ttgttcgatt
     1201 ccggtgaagt ctgcgcgttt cgtatcaaat accccaacgg tacggttgat gttttccgtg
     1261 gctggctgag ttcactgggt aaaaccatta cctcaaaaga ggtgatgacc cgtactgtaa
     1321 aaatcagcgg tgtggggcgt ccgtatctgg cagaggaggg cgctgaaacc gtgggtgtta
     1381 ccggactgac ggtggcaccg gcatctgtca gtgtcaaagc gggagcaacc accacactga
     1441 cctttacagt aaaacctgac ggggccagtg acaaagcgat cagtgtgcat tcgtcagatc
     1501 ctcagactgc ttcggtgccc ctgagcgggc ttgtggccac ggtgaaaggc gtgcagcagg
     1561 gcagtgtcag catcgtgggc atgacctctg acggagagtt tgtggcagtg gctgcggtga
     1621 ccgtcagcgc accataacag gacgatactc atcattgccc cggttatccg gggctttttg
     1681 catccggaga acatgatgtt tctgaaacag gatacgttta attatgaaaa acagtccgtg
     1741 gtgctcagtg agctgtccgg gctgcagaga attgaatatc tgacgtttgt tcagcagcga
     1801 aaggcaaagt ttgatgcaca ggagggagaa ctgccggagg ctgaacgaca gattgctttt
     1861 ctgcgtatgg gaatggatat caatgcctgg ctggtttccc gctcactgtg gaatgctgag
     1921 cagtctcagg atgttgagac gctttgcgca tccattatga caacatggtc gtatgatgcg
     1981 ctgggcgcgg gggcggagag ggttctgtcg ctgagcggta tggggaccat tgagaatgcc
     2041 ggggatgatg atcatgaggc gctgacgccg gaaaagtcct gacgcgggaa atgcagtttg
     2101 tcatgcggct tgcccgggag ttccggcggg cagactggcg gcagatgctg tcggatatgt
     2161 cggccactga gcttggtgag tggggcgatt atttccggat gcagagcttc agtgatgtgt
     2221 ggatggatgc gcagtttgcc tcgctgaagg cattgatcgt gagaatggtg tccggcagca
     2281 gcgatgctgc ggtggctgat ttcagccttt taccggaaga gaacgggata ccggagcgaa
     2341 cggacgaaga actgatgcat cttggggaag gtatttccgg aggtgtgcgt tatggaccag
     2401 atagccaacc tggtcattga tttggggatt gatgcggcag agtttaaaaa tgaaattccc
     2461 cgtatcaaaa accttctgaa tggtgcagcc agcgatgcag aacggtcttc tgcccgtatg
     2521 cagcgtttta tggagcgtca gactcaggcg gcccggcaga caatgcaggc ggcttcttcg
     2581 gctgcaacag cagcatcagc ccatgcgcag acggtggaga agaatgcacg ggctcatgaa
     2641 cgcatggccc gtgaggtgga acaaacccgt ctgcgcgtgg atgccctgaa tcagaaaatg
     2701 cgcgaggaac aggcgcaggc cagggcactg gcggaggcgc aggataaagc ggctgccgcc
     2761 ttttatcgcc agattgacag tgtgaaacag gccggtgcgg ggcttcagga attacagcgt
     2821 attcagcagc agatccgaca ggccagaaac agtggcgggg ttggtcagca ggattatctg
     2881 gcgctgattt cggagatcac ggcgaaaacc cgtgccctga cgcaggcaga ggaacaggcc
     2941 acccggcaga aagcggcgtt tatccgccag cttaaagagc aggcaacccg ccagaatctg
     3001 tcgtcttctg agttgcttcg cgccagggcg gctcagctgg gggtgttaat cagtcagatg
     3061 gcgcgcggca attttggtgc gctgagggga tccgggataa cgctggctaa ccgtgccgga
     3121 tggacaggcg cactgatgtc gccgaaaggc atgatgactg gcggcgttat tggcggactt
     3181 gtcgcggcgg tcctgggtct gggtaaagcc tggcatgacg gcggaaggag ggcgaggaat
     3241 ttaaccgtca gctggcgctg acgggacatt atgccggtgt cactgtcggg cagttgtgga
     3301 aactcagccg ggccatatcc gggaatggta tcacgcaaca tgcggcagcc ggtgcgctgg
     3361 cacaggtggt gggcagtggg gcatttcatg gaaacgatat tggtatggtg gcgaaagctg
     3421 ccgcacagat ggagcgatcg gttggccagt cggtcagcga taccataaat cagtttaagc
     3481 ggctgaagga tgatcctgta aatgccgcga aggcgctgga caatgcgctg cattttttga
     3541 ctgccactca gcttgagcag atacgcgtcc ttggggagca ggggcggtcc agtgatgcgg
     3601 cccggatcgc catgtctgcg ctggcagagg aaaccggtaa acgcacatct gatattgata
     3661 ataatctcaa tgcgctgggt agtacgctgc aaaccttgtc tgactggtgg aagcagttct
     3721 gggatgcggc catgaatatt ggtcgtgaag actcgctgga tgcgcagatt gatgcgttac
     3781 aggaaaaaat tcagcgcgcg aaaaaatatc cgtggacaaa cgcctccaca caggtggagt
     3841 acgatcagca gcgtcttaac gatcttcagg agaaaaaacg ccggaaggat ttgcaggatg
     3901 caaaagcgca ggcagaacgg aattaccagg agcaacagaa acgccggaat gctgaaaatg
     3961 ccgcgctgaa ccggatgaat gaaacggaag ctgcacgaca tcagcgggaa attgcgcgta
     4021 ttaatgccat gcagtacgcc gaccaggctg tcagggatgc ggcgatacag cgtgaaaatg
     4081 aacgttacga aaaagccatt aagaaaaata cacgggcaac ccgtaatgat gaggccaccc
     4141 ggttattgct gcagtacagt cagcagcagg cacaggtgga agggcagatt gctgctgcca
     4201 gacagtcagc aggcattgcc actgaaagga tgactgaagc gcataaacag cttctggccc
     4261 tgcagcagcg catcagcgac ctggacggga aaaagctgac ggcagatgaa aagagtgtgc
     4321 tggcccgtaa gaatgagctg attcaggcgc tgacgctgct ggatgtgaaa cagcaggagc
     4381 tgcagaaaca gacagcgctt aacgacctga gaaaaaaaac ggttcagctg accagccagc
     4441 tggcagacaa agaacgtgca ctgcgtgagc agcacaatct ggatattgcc actgcaggta
     4501 tgggggataa gcagcggcag cgctaccagg cacagttgcg catccggcag gaataccggc
     4561 aacagttgca acagcttgag aatgacagtc gccagaaagg cacttacggg acggaggact
     4621 accggagggc tgaggaggtg ctgaagggga gcctgaagcg acaactgaat gaaaacaaac
     4681 gctactggca ggaactggaa gtggcgcagg gcgactggaa aaacggtgcc atgcgggcgt
     4741 ttcagaattt tacggcggat gcggataatg cggcgggaac ggcagaacag atgtttacag
     4801 tggcattcag cagtgccggt aatgcactgg cgacattctg taccaccggt aagctgaatt
     4861 ttaaatcctt cacctcttcc ctgttgtcag atatggccag aattatggca cagatggcca
     4921 tgatgcaggc ggtaaagggc gtcggttctt tattcggctt cacgactaat gctgatggcg
     4981 gtgtttacca gtctgctgat ttgagtcgct acagtggcac ggtggttaac cgtccgacgt
     5041 tttttgcttt tgcaaaaggc gcgggtgtga tgggggaagc gggacctgaa gccattctgc
     5101 cactgcgtcg cggtgctgat ggtaagctgg ggggttgtgg cggatactgg tggttcaggc
     5161 atggtgatgt ttgccccgca gtacaacatt gagatcaata acgacggcac gaacgggcag
     5221 ataggtccgg ctgccctgaa ggtggtttat gacctcggga aaaaagcggc cgcggacttt
     5281 atgcaacagc aggcccgtga tggtggccgg ttaagtggag cataccggta atggagacgt
     5341 ttcactggaa agtgcgcccg gatatgaatg tggtatcaga gccgaaagtg gtgacagtga
     5401 agctgggcga tggttatgaa cagcgtcgtg cggcgggact gaataaccag ttgtcgactt
     5461 acagcgtgac gatacgtgtt cgtaaaggtg aacacccatc tttaaaagcc tttctggaac
     5521 ggcacggtgg cgtccgcgca tttcagtgga cgccacctta tgactggaaa ctgatcaggg
     5581 aggtttgtcg taaatggtcg gcaagcgtgg gggcgttatg ggtgactgta acggccgatt
     5641 ttgaacaggt ggtgaactga tgcaggatat ccggcaggaa acactgaatg aatgcacccg
     5701 tgcggagcag tcagcccggg tggagctctg ggaaatcgat ctgacagagg tcggtggtga
     5761 gcgttatttt ttctgtaatg agcagaacga aaaaggtgag ccggtcacct ggcaggggcg
     5821 gcagtatcag gcatacccca ttcagggggc ggggtttgaa ctgaacggca ggggctgtgc
     5881 tgcccgtccg acactgacgg tctctaacct gcacggcatg gtcaccggga tggcggaaga
     5941 tctgcagagt ctggtcggcg gaacggtggt ccgccgtaag gtttacgacc gttttctgga
     6001 tgcggtgaac ttcgtcaacg gaaacagcga cgccgatccg gaacaggagg tgatcagccg
     6061 ctggcgcatc gagcagtgca gcgaactgag cgcggtcagt gcctcttttg tgctggccac
     6121 accaacggag acggatggcg cggttttccc ggggcgtatc atgctggcga atacctgtat
     6181 gtggacttac cgttctgatg agtgtggtta cacgggcagg gcagtggctg acgagttcga
     6241 caaaccaacg acggatatcc ggaaggacaa atgcagcaag tgtatgcgcg ggtgtgagtt
     6301 gcgcaacaat accggtaatt tcggcggttt cctttccatc aataaacttt ctcagtaaat
     6361 ccatgacaca gacagaatca gcgattctgg cgcacgcccg gcgatgtgcg ccagcggagt
     6421 cgtgtggatt cgtggtgaga acgccggagg gagacaggta tcttcccagc gagaatatct
     6481 ccggtgagcc ggaggaacgg ttccggatgg ctccggagga ctggctgcgg gcacaaatgc
     6541 agggtgagat tgtggcactg gtccacagtc atcccggtgg tctgccctgg ctgagtgagg
     6601 ctgaccggcg gctgcaggtg cagagtgatt tgccgtggtg gctggtctgc cggggggcga
     6661 ttcacaagtt ccgctgtgtg ccgcatctca ccgggcggcg ctttgagcac ggggtgacgg
     6721 actgttacac gctgttccgg gatgcttatc atctggcggg gattgagatg ccggattttc
     6781 accgcgggga tgactggtgg cgtcacggtc agaatctcta tctggataat ctggaggcca
     6841 cagggctgta tcaggtgccg ttgtcatcag cacaaccggg cgatgtgctg ctgtgctgtt
     6901 ttggttcatc ggtgccgaat catgccgcca tttactgcgg cgatggtgag ctgctgcacc
     6961 atattcctga acaactgagt aaacgagaga ggtataccga caaatggcag cgacgcacac
     7021 actccctctg gcgtcaccgg gaatggcacg catctgcctt tacggggatt tgcaacgatt
     7081 tggccgccgc atcgaccttc gtgtgaaaac gggggctgaa gccatccgcg cactggccac
     7141 acagctcccg gcgtttcgtc agaaactgag tgacggctgg tatcaggtgc gcattgccgg
     7201 gcgtgatgca ggtgaaaccg aattgtctgc ccgtcttaat gagccgctgg caaatggtgc
     7261 agtgatccac atagtaccgc gtctggtggg agctaaaagt ggcggtgtgt ttcaggcggt
     7321 gctgggggca gctgttatgg cggttgctat atggatgccg gggtaggaat tatggcgagt
     7381 aatctgctgt tttctctcgg tgccagtatg acgcttggcg gtgttgcaca gatgctggca
     7441 ccgaaagcca gaactccccg tacacagaca acggataacg gcaaacagaa cacctgtttc
     7501 tcctcactgg ataacatggt tgcccagggc aatgttctgc ctgttctgta cggtgaaatg
     7561 cgcgtggggt cgcgggtggt atctcaggag atcagcacgg cagacgaagg ggatggtggt
     7621 caggttgtgg tgattggtcg ctgatgcaaa atgtttcatg tgaaaccgcc tgcgggcggt
     7681 tttgtcgttt atggagcgtg aggaatgggt aaaggcagca gtaaggggca taccccgcgc
     7741 gaagcgaagg acaacctgaa atccacgcag ttgctgagtg tgattgatgc catcagcgaa
     7801 gggccggttg acggtccggt ggatggatta aaaagcgtgc tgctgaacgg tacgccggtc
     7861 ctggacagcg aggggaagac aaacttttcc ggtgttacgg tggtgttccg cgccggcgag
     7921 caggagcaga caccgccgga ggggtttgaa tcttccggct cagagactgt gctgggtacg
     7981 gaagtgaaat acgacacgcc gatcacccgg accatcacgt cggcaaacat tgatcgtctg
     8041 cgcctgacct tcggtgtgca ggcactggtg gaaaccacct caaaggggga ccggaatccg
     8101 tcggaagtcc gcctgctggt tcagatacag cgtaacggtg gctgggtgac ggaaaaagac
     8161 atcaccatta agggcaaaac cacttcgcag tatctggcct cggtggtggt gggtaacctg
     8221 ccgccgcgcc cgtttaatat ccggatgcgc aggatgacac cggacagcac cacagaccag
     8281 ctgcagaaca aaacgctctg gtcgtcgtac accgaaatca tcgatgtgaa acagtgctac
     8341 ccgaacacgg cgctggtcgg cgtgcaggtg gattcagagc agttcggtaa ccagcaggtg
     8401 agtcgcaatt atcatcttcg cgggcgcatt ctgcaggtgc cgtcgaacta taacccgcag
     8461 acgcggcaat acagcggtat ctgggacgga acgcttaagc cagcatacag caacaacatg
     8521 gcctggtgtc tgtgggacat gctcactcat ccgcgctacg gcatggggaa acgtcttggt
     8581 gcggcagatg tggacaaatg ggcgctgtat gtcatcggcc agtactgcga ccagtcagtg
     8641 ccggacggat ttggcggcac ggagccgcgc atcacctgta acgcttacct gaccacacag
     8701 cgtaaggcgt gggatgttct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg
     8761 aacgggcaga cgctgacgtt cgtgcaggac cgaccgtcgg ataaagtgtg gacctataac
     8821 cgcagtaatg tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg
     8881 aaggaccgcc ataatgtcgt tgaggtgaac tggattgatc cggacaacgg tcatgagacg
     8941 gcgacagagc ttgtggagga cacgcaggcc attgtccgtt acggtcgtaa cgtcacgaag
     9001 atggatgcct ttggctgtac cagccgggga caggcgcacc gcgccgggct gtggctgatt
     9061 aaaacggaac tgctggaaac gcagaccgtg gacttcagcg tcggcgcaga agggcttcgc
     9121 catgtaccgg gtgatgtcat tgaaatctgc gatgatgatt atgccggtat cagcaccggt
     9181 ggtcgcgtac tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg
     9241 ctgccgtcct ccggtaccac gctgataagc ctggttgacg gaaatggcaa tccggtcagc
     9301 gtggaggttc agtccgtcac cgacggcgtg aaggtgaaag tgagccgggt tcctgacggc
     9361 gttgcagaat acagcgtgtg ggggctgaag ctgccgacgc tgcgccagcg cctgttccgc
     9421 tgcgtgagta tccgtgagaa cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg
     9481 ccggaaaaag aggccatcgt ggataacggg gcacactttg acggtgaccg gcgcggcacg
     9541 gtgaatggtg tcacgccgcc agcggtgcag catctgaccg ccgaagtcac cgcagacagc
     9601 ggggaatatc aggtgctgga gcgatgggac acgccgaagg tggtgaaggg tgtgagcttc
     9661 ctgcttcgcc tgaccgtggc agcggacgac ggcagtgagc ggctggtcag cacaggcagg
     9721 acgacggaaa ccacataccg cttcaggcag ctggcgctgg ggaattacag tctgacagtc
     9781 cgggcggtaa atgcccgggg gcagcagggc gatccggcgt cggtatcgtt ccggattgcg
     9841 gcaccggcag cgcctgtcac tattgaactg ataccggggt attttcagat aacggtggtc
     9901 ccgaaactgg ctgtatatga cccgacggtg cagtttgagt tctggttttc ggaaaagcgg
     9961 attgcggata tcaggcaggt tgaaaccagc gcccgctatc ttggcacggc gctgtactgg
    10021 atagccgcca gtatcaatat caggccgggc catgattatt atttttacgt tcgcagtgtg
    10081 aacaccgttg gcaaatcggc atttgtggag gccgtcggtc gggcgagcga ttatgcggaa
    10141 ggttatctga gtttttataa agggttgatc aataaaacgc atctcggcaa ggagttgtgg
    10201 acgcagattg ataacggtca gcttgcgccg gacctgactg aaatcaggac gtccattacg
    10261 aatgtcagca atgaaatcac gcaaaccgtc aataaaacac tggaaaatca gagtgctgca
    10321 atccagcaga tacagaaagt tcaggttgat acaaataata acctgaacag catgtgggct
    10381 gtgaagctgc aacagatgaa ggacggacgc ctttatattg cgggtatcgg tgccggtatt
    10441 gagaatacgc cagcaggaat gcagagtcag gtgctgctgg cggcagacag gattgcgatg
    10501 attaatcctg cgaatggcaa cacaaagccg atgtttgttg gtcagggcga tcagatattc
    10561 atgaacgacg tgttcctgaa acgcctgacg gctccgacca ttaccagcgg cggtaatcct
    10621 ccggcatttt ccctgacacc tggcggacgg ctgacggcga aaaatgccga tatcagcggt
    10681 aacgtgaacg cgaactccgg gacgctcaac aacgtcacga ctaataagaa ctgtcgggtt
    10741 ctgggaaaat tgtccgcgaa ccagattgaa ggcgatctcg ttaaaacagt gggcaaacct
    10801 ttcccacggg actcccgggc accggagagg tggccatcag ggaccattac cgtcagggtt
    10861 tatgacgatc agccgtttga tcggcaaatt gttattcccg cggtggcgtt tcgcggtgct
    10921 aaacatgagc ggaagaataa caatatttat tcgtcatgcc gcctgatagt gaagaaaaac
    10981 ggtgctgaaa tttataaccg aacgaccctg gataatacgc tgatatatac gggtgttatt
    11041 gatatgcctg ccggtcacgg tcacatgacg ctggagtttt ctgtatcggc atggctggta
    11101 aatggctggt atcccacagc aagtatcagc gatttgctgg ttgttgtgat gaagaaagcc
    11161 actgcaggca tcacgattag ctgaatttta taacccagat acgggcacca gaaatggtgc
    11221 cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa gtttgtgcag ccattttgtc
    11281 cgcagccatc tgtctggctg tatccggtgc gcctgcatgg gcgtctgaac atcagtccac
    11341 gctgagcgcc gggtatcttc agccccatac tgatatgcca ggcagcgatg acctgaaggg
    11401 cattaacgtg aaataccgtt atgagtttac ggacacgctg gggctggtga cgtcattcag
    11461 ctatgccaac gctgaagatg agcaaaaaac gcattacagc gatacccgct ggcatgagga
    11521 ttccgtgcgt aaccgctggt tcagcgtgat ggcggggccg tctgtgcgcg tgaatgaatg
    11581 gttcagcgcg tatgcgatgg cgggcgtggc ttacagccgt gtgtcgacct tctccgggga
    11641 ttatctccgc gtaactgaca acaaggggaa aacgcacgat gtgctgaccg ggagtgatga
    11701 caaccgccac agcaacacgt ctctggcgtg gggggctggt gtgcagttta acccgaccga
    11761 atccgtggcc attgaccttg cttatgaagg ttccggcagt ggcgactggc gcactgacgg
    11821 tttcattgtg ggtgtcggtt ataagttctg attagccagg taacacagtg ttatgacagc
    11881 ccgctggttc aggcgggctt tttgtggggt gaatatggca gtaaagattt caggtgtact
    11941 gaaagatggg gcgggtaaac ctgtcgtaaa ttgtgcgatt gaactgcggg ccagaagaac
    12001 cagtccgacc gttgtggcac acgttgttgc cacttgcgtg acggacaata acggtgctta
    12061 tgtgattgag gctgagccgt ggtattacga ggttgcgctt cactgtaacg gctggcagcc
    12121 aacccgtgtc ggggatattg atgtggcacc gactgatgca ccggggacac tgaacgcgtt
    12181 tctgaatgca ccaaaggatg gtgatttacg tccggaggtg atgaagcgct ttgaggaaat
    12241 ggtggcgcag gcgcagcaga gtgccggggc
//
LOCUS       DAJTXY010000114        12024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTXY010000114 DAJTXY010000000
VERSION     DAJTXY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.114"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..867
                     /locus_tag="OO566_003269"
     CDS             <1..867
                     /locus_tag="OO566_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR6606445.1"
                     /translation="AISIKGVNTGVIRKSNNFIALPLKIKEPRNKESLFFMSVMELRD
                     LLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTL
                     ELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLL
                     DFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTR
                     EATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYARTVAANNAQPLTQDQCLRALHHLR
                     VQSTSKTAPQAK"
     gene            complement(1070..1423)
                     /locus_tag="OO566_003270"
     CDS             complement(1070..1423)
                     /locus_tag="OO566_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6606446.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1561..3207)
                     /gene="groL"
                     /locus_tag="OO566_003271"
     CDS             complement(1561..3207)
                     /gene="groL"
                     /locus_tag="OO566_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6606447.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3251..3544)
                     /locus_tag="OO566_003272"
     CDS             complement(3251..3544)
                     /locus_tag="OO566_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6606448.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3820..5076
                     /gene="yjeH"
                     /locus_tag="OO566_003273"
     CDS             3820..5076
                     /gene="yjeH"
                     /locus_tag="OO566_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6606449.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5092..5568)
                     /gene="fxsA"
                     /locus_tag="OO566_003274"
     CDS             complement(5092..5568)
                     /gene="fxsA"
                     /locus_tag="OO566_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6606450.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5905..7341
                     /gene="aspA"
                     /locus_tag="OO566_003275"
     CDS             5905..7341
                     /gene="aspA"
                     /locus_tag="OO566_003275"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6606451.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7459..8760
                     /gene="dcuA"
                     /locus_tag="OO566_003276"
     CDS             7459..8760
                     /gene="dcuA"
                     /locus_tag="OO566_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6606452.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8876..9214
                     /gene="cutA"
                     /locus_tag="OO566_003277"
     CDS             8876..9214
                     /gene="cutA"
                     /locus_tag="OO566_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6606453.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9190..10887
                     /gene="dsbD"
                     /locus_tag="OO566_003278"
     CDS             9190..10887
                     /gene="dsbD"
                     /locus_tag="OO566_003278"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6606454.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10924..11499
                     /locus_tag="OO566_003279"
     CDS             10924..11499
                     /locus_tag="OO566_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6606455.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11606..11681
                     /locus_tag="OO566_003280"
     tRNA            11606..11681
                     /locus_tag="OO566_003280"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11639..11641,aa:Phe,seq:gaa)
BASE COUNT         2883 a         3120 c         2967 g         3054 t
ORIGIN      
        1 gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa tttcattgca
       61 ctgccgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat gtctgtcatg
      121 gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca taaactggat
      181 gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa aatggcagaa
      241 aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg tcgggttaac
      301 acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac gctacatgac
      361 ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc gcgagcaatc
      421 attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac ctcactgtta
      481 gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga atatgacacc
      541 tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt gctttaccgc
      601 tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac gcgtgaagca
      661 acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag cccacgactg
      721 aaaaaactcg cgggtgtgcc atgtcaggtc tatgctcgca ccgtagcggc gaataacgcc
      781 caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt acaatccacc
      841 agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc tggagccatc
      901 cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt aaacgcctta
      961 tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag gcctgataag
     1021 cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat cagttcgggc
     1081 acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac aacattccct
     1141 gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc tggttgcgca
     1201 gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg gaaagccagt
     1261 tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc tgctcgtcca
     1321 caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa cccgccatca
     1381 gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc cccataatca
     1441 atgagctgga cgccactcgc gtcgtccgtg tctgaatctt atactagaaa gatgaccaaa
     1501 agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag tgcagggcaa
     1561 ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc ctaagtcagc
     1621 tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca ggccagccac
     1681 agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga tacccatgtc
     1741 gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt cgccgccttt
     1801 aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct gacgcagcgg
     1861 agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt tctgaccacg
     1921 caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag caaccacgcc
     1981 ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt ttttctcttt
     2041 catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg ccagtttcgc
     2101 tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa tctgctgacg
     2161 gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga tgatagtggt
     2221 ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg ttgctttttc
     2281 cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg cgatatcctg
     2341 cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa ctttcacgat
     2401 gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat cttcagcgat
     2461 gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca tttcgcggat
     2521 gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta ctgcgccagt
     2581 ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca taccttcaac
     2641 cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc cttctttacc
     2701 gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt tagcggagat
     2761 ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg ctttcagttc
     2821 ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg ggttcatgcc
     2881 cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg ttgcagtggt
     2941 ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca tctgcgcacc
     3001 catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac catctttggt
     3061 gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg gaccgagggt
     3121 aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac gagcgtcgtt
     3181 accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta tgttcagtgt
     3241 cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg atcaacactt
     3301 cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata acgatgtcgc
     3361 caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca ttgccgacag
     3421 ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg atgccgccag
     3481 cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat ggacgaatat
     3541 tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc ggagcttacg
     3601 tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc aagggggaaa
     3661 aagaaaaaaa attctgaaca gcacaaaatc gggtgaaaac cctgattcac ctcacatttc
     3721 atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc gaaaatctac
     3781 gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact caaacaagaa
     3841 ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac tggcgtgttt
     3901 gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc gtggcccgtt
     3961 ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg ccactatccc
     4021 agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct tgagcgagtc
     4081 accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact acaaattgcc
     4141 gccggattcg gccaggcaat gtttggctgg catagctggc aactgttgtt ggcagaactc
     4201 ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag tgctaatcta
     4261 caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg ggcgggcgat
     4321 atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac cgggttattt
     4381 gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc ccatcttgcc
     4441 tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg tctgctgctg
     4501 gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc ctatggtgaa
     4561 caaatggcgg cggcagcatc gcttccaaaa attgtagtgc agttgttcgg tgtaggagcg
     4621 ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa catttatata
     4681 cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca ctacctggca
     4741 cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg ctgctgcgtg
     4801 gtgagcacgt tggtaattca tgccttagag atcaatctgg aggctcttat tatttatgcc
     4861 aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa attattgcaa
     4921 ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt actggcaatg
     4981 gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct gtttctgcca
     5041 aaacgaaaaa cgccggaaaa tggcataacc acataatccg gcgtttcgac attaatcctg
     5101 gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat actcaccatc
     5161 aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc gagaaaagcg
     5221 caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata aaagcagaag
     5281 accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa taatcagcga
     5341 aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt gctgcatcag
     5401 cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg acgtgaatat
     5461 aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa agattgaaat
     5521 ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag gtttctcctg
     5581 taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcgtt cagcttatat
     5641 tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt aatcccaaag
     5701 cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta aagtgatcca
     5761 gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga tgcaggggat
     5821 aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa gcaaatcatt
     5881 ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga agatctgttg
     5941 ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag agcgattgaa
     6001 aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg cggtatggta
     6061 atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc taaaagtgta
     6121 gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg catggatcag
     6181 ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa caccaacgaa
     6241 gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata tcagtacctg
     6301 aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc gaccggtttc
     6361 cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca actgcgtgaa
     6421 ggctttgaac gtaaggctgt cgaattccag gacatcctga aaatgggtcg tacccagctg
     6481 caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat cctgctgaaa
     6541 gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa ccttggcgca
     6601 acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc agtgaaaaaa
     6661 ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga agcgacctct
     6721 gattgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt gaagatgtcc
     6781 aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct gaacgagatc
     6841 aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa cccggttgtt
     6901 ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac tgttaccatg
     6961 gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg ccaggccatg
     7021 ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa atgcattaac
     7081 ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat cggtatcgtt
     7141 acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa aatctgtgcc
     7201 gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac tgaagcggaa
     7261 cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc aaaacgctat
     7321 actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc acgtcagatg
     7381 acgtgccttt tttcttgtga gcagtaactt aaaaataaca atctaatatc aacttgttaa
     7441 aaaacaagga aggctaatat gctagttgta gaactcatca tagttttgct ggcgattttc
     7501 ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg ggtgctggtt
     7561 cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc cattatcatg
     7621 gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta tctggttcat
     7681 cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc accgatcgtg
     7741 acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac actgccagtt
     7801 atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc tactgcagtg
     7861 gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt ttacatgtct
     7921 tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt catcccgtcc
     7981 accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa ctccaaactc
     8041 tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact gcgcggtgaa
     8101 aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct gctgggcgta
     8161 gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt tgaaaaaccg
     8221 ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac tctgaccacc
     8281 gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa agcaggtatg
     8341 agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt ttccaacaac
     8401 atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg gctgctggcc
     8461 gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc aaaagcactg
     8521 atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc tttcgctgcg
     8581 gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt acagatggat
     8641 gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt catcccgggt
     8701 actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt catgctgtaa
     8761 tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca tccttccccc
     8821 gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg tgtttatgct
     8881 tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac cggatgaagc
     8941 gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct gcgcgacctt
     9001 gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag aatacgaagt
     9061 gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat gcctgaagtc
     9121 tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg gagacacaga
     9181 ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag cacttccgtt
     9241 tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga tcaagccttt
     9301 gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat caaagacggt
     9361 tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat tgccgacgtg
     9421 cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga gatttaccgc
     9481 gatcggctga cgcttcccgt aaccatcaac caggcgagtg cgggagcgac gttaactgtc
     9541 acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa aaccgttccg
     9601 ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc gcagcaagag
     9661 cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg tattggtatc
     9721 gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt gctgggtggt
     9781 aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt gcaggggatg
     9841 gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca gttccaggcg
     9901 gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt gctggcgatg
     9961 tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg tctcacgttg
    10021 atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg ggcgattgcc
    10081 ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct gtatatcgcc
    10141 caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt gggcatgggc
    10201 ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag cggcccgtgg
    10261 atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt cttcctgctg
    10321 gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg tgtcgcattc
    10381 tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg cgtggtgcaa
    10441 ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg ggcatttggt
    10501 gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa aacgatagat
    10561 gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga tctttatgcc
    10621 gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc gcaggtgcaa
    10681 aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga cgcacaagat
    10741 gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt ttttgacgga
    10801 caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga aaccttcagc
    10861 gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac ggaggaaaat
    10921 agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt acaagggatt
    10981 gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga cgagctacgc
    11041 cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct tagccaacag
    11101 atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga acaaaagctg
    11161 ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc gggctgtcta
    11221 tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca actggccgat
    11281 cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacgct ggaagttgac
    11341 gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt aagccgaatg
    11401 ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga agatatcctg
    11461 cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag aaaagaagcg
    11521 atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga cgagatgtgc
    11581 agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc aggggattga
    11641 aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat caacggacct
    11701 ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc ctccaaatca
    11761 attctaccga actcaaccag attctcccca catcaccagc aatttgcgag catatcccaa
    11821 tttgggaaaa tttgttcctg agctatagca ctaactgacg tgaaatgtcg tgcggccccg
    11881 tgatgctgtt gaacgtcaaa tgacgtcatc aggagcgtaa cgtatccata aagcacaaca
    11941 ccggtctgaa cgccgactga tgagattttc tgaatgagaa caaagagaaa tgtgtcagtc
    12001 cgcttgctca tgcaaagact aaca
//
LOCUS       DAJTXY010000115        11928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTXY010000115 DAJTXY010000000
VERSION     DAJTXY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.115"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(1..2249)
                     /locus_tag="OO566_003281"
                     /pseudo
     CDS             complement(1..2249)
                     /locus_tag="OO566_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(2273..2896)
                     /locus_tag="OO566_003282"
     CDS             complement(2273..2896)
                     /locus_tag="OO566_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR6606456.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVINH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2893..4581)
                     /locus_tag="OO566_003283"
     CDS             complement(2893..4581)
                     /locus_tag="OO566_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCR6606457.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAVGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(4730..7357)
                     /gene="gyrA"
                     /locus_tag="OO566_003284"
     CDS             complement(4730..7357)
                     /gene="gyrA"
                     /locus_tag="OO566_003284"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6606458.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7504..8226
                     /locus_tag="OO566_003285"
     CDS             7504..8226
                     /locus_tag="OO566_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6606459.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8354..11928)
                     /gene="yfaL"
                     /locus_tag="OO566_003286"
                     /pseudo
     CDS             complement(8354..11928)
                     /gene="yfaL"
                     /locus_tag="OO566_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2767 a         3309 c         2939 g         2913 t
ORIGIN      
        1 tcgacattga ccagttcacc aggctggtaa tgggttttgt ccgttttcac ccggatatca
       61 agctgaggaa cggcaacttt gatcccggcg ttctgaaaac tgtactgacc attacgggta
      121 tacagcaccg aaaaagtgat gttaggcgca aaggaattgc tcactggaac ccgggcttca
      181 tacagggtat cgtttaaacg ttgtagcgtt agccagtttg ccggatgcga aagcagcgac
      241 tgctgttcca cgcgatcgcg ttccagcgtc aataacgctt catcaattgg ctccgggaaa
      301 gtaatcagca tcttcgcggt ttcgcctggc tggtacagcg ttttatccgc cacgatatct
      361 accgtaccag tatgcgccgt gctgccctta ccgctgacgg catgacttaa cccagcgaga
      421 attaagccgt ctttatcgcg taatgtcaga ttgtagttgc caggtttagc gaaattgacg
      481 gtaaaggatt tgccgcctga cggtagctct ccgctatggc tcgtgcggtc ttcgagacgc
      541 aaccattcat acgtaacagg aacctgtttt gaagattcca gcgcggcata acggaacaca
      601 accgactcgc cgctattact gtattgtgcg gcggtactta aagagtaatg cgccaggccg
      661 cgttcaatga ggagctcttt ggtggtggtg acgcgatacg ccgcgccgtc actggcggag
      721 acggttaaca aatagcggct cggtttatcg gcggcgggga gatttaacgc cacataaccg
      781 ctggcgtcgg acaccgtttc gctgccttcc agcgacacgg ggaaacgtcc ggcataacgc
      841 aaatcgttac cgaccattga taattgctga gcgcgcaaac ttaactgcac gcgggcattt
      901 tttaccggct cgccatccgg gtagagcagt tgcagtttgc cgctgaccgc ttcgccagtt
      961 ttgaactctt ttttgtcgag agctaaacca atctcgaaat gtggcttgat gtagtttgcc
     1021 acgcgaaaac tgctgctata gacctgattg cggtaagcaa gacgtaactc ataacctccg
     1081 gcaacggcat tttctggcag gcggaaactt ccctgcccgc cattgcgcgc atccagcgtg
     1141 acatcgacgg tttgcaacag actgccgtta gcgtccagca ccgaaagctt cgccggggcg
     1201 ctgacgatgg gggatgaatg caacggatcg cggaactcgc ggcccatcac tttaacatcg
     1261 acacgatcgc ctgcgcgata tagcgggcga tcggtaaaaa tatacaagcg ggtgttgtag
     1321 atttcgcttt cgtagaagaa gttctcggag acaaaaacgc cgccttcagc atccttaccc
     1381 agaatgtatg aacgttctgg cgatatatgt tgtaactgca aagtaccgct gtcatcggtc
     1441 accccgcggg tcatcacgcc aagaccgtca gtccacaaga tctctgagcc gggcttcgct
     1501 tcaccctgtt ttttacccgc ggtccatacc agaagctcgt tacctgacac tttgctaagc
     1561 gccacggtat cggaaacaaa caccaccgtc gtcgcccgat acccacctac catcgcctcg
     1621 acgaggtaca gccccggctc ttgttggccg agaggaatat aaatattacc cggctgcggc
     1681 gagatgaaat tgctggatgc gccttccagt tttatccctt gctgcggctc gaccggttta
     1741 gcctgccata gtggataacg gaactgttcc accaagggat attttttcag cggggaaaac
     1801 tggttgttct gtacataacg ggaaggttta ataatggcat tgccgagatg taattcgggt
     1861 aatgcctgag tcacattctg ccgtgactga gaagagaaag tacgctgcat cacgcggcga
     1921 gatttgccgt accagttatc ccacagctag gtcagcgtat tgttcagccc gtcgcccaga
     1981 tattgcggtt gcaccacaat gcgatgcagg tttttctgct ggcgcaaaaa tgccatcggg
     2041 tcaggaatac gatacaggcg aacgtccacg ccgccgtact cttccatctg ataacgccga
     2101 taatcacgcc ccggcgcttc cagtcgcact ttcgcctctt cactgctgct aaaactgctg
     2161 tcagcaagca aaaagaatgt tcccccggcg ggcggcgcat agttactgga aggaagcgaa
     2221 tcatcagctt tagcaagccc tgttcccaca agagacaggg acaagcacgc tgctatcgcg
     2281 ccagaaaatt taaacgataa atgccaatga aattgggatt ggaatcgttg ggtatccatc
     2341 gggtgtcctt ccatgtcata agttgttgca gactgactgc gcgcattccg ttgtcagttt
     2401 tcgtggcgct tccggtgtgg ttgatgacgt aacgccccat ccagaccatt aagtgctggg
     2461 catcgccctg atcgaaaaaa atcatatcgc caggcagcgc ctggtttatg tcttggccaa
     2521 taaactggct gttgtactga atcaaattaa tcgcggtcac gtaggggccg tttttcccgc
     2581 tcccctgatt ccagttttgc gccagttgac gctgttcagg tgttagcgtc atctctggcg
     2641 gcaaatactg gctagataaa ccgttacttt ttagccattt actgtcgtga actttcagcg
     2701 tctcgttcgc cgcaaatcgc accaggcccg cacaatcctg ctgataccag cgtggacttg
     2761 gcccctggcg gagctgttct tgtgcaatgc gtacaaacca ggcgcgaaac agcctggatt
     2821 gttcgacatt cagcatttcg ctatgggcaa caacacaaca cagccagcaa atcagcgcca
     2881 gcagcccgtg tctcatagcg gctgccaggt tatgggtagc cactgccagg cgacaccggg
     2941 ttccatctgg gccagcttca tgacataacg cggttgttga gataaagcgt ccagcttcgg
     3001 cattaataaa gtttgtgcgg cgttataaaa aaccggttcg agattcttcg gcagactggt
     3061 caacgtttcg ttacgcagca gtttcgccac gccttgtgga ttgatataga gcggaacgat
     3121 gccatcagtg ggtattacat ccaccattgc cgggcgggtt ttattcagtg tttgcagggc
     3181 gttattaacc agcgtgtcat cgagggagaa aagcagcgtt ttgttttgca tcgccagcga
     3241 cacacggaaa aaataatccg acattaattg atcgggttgc gccgcctgcg ctttcggata
     3301 ctgaccgtat cgggaactca cttcgcgaca ccagatttgc gcttcgccct gctgagtctg
     3361 gcttaccggc aaaacacctt ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa
     3421 cagtttccct ggcaattgcg cctgttcggc agtgccatca aactgaccga caaacagcgg
     3481 ggtttgcaat tttgagtctt cataccagca cagccccacg gcaccgtcta acgccccatt
     3541 cagcttgtcg ttttcctggc tgatgtgcga aagcatctct tcggcaatac cgtgtgaata
     3601 cggcaccgcc acacagaagc tggctccggc aggcatactg ttccagaccg gcgtaaaatc
     3661 gaaactggca tctacgctgg cggattcatc atttaacgcc acaaaactgt gccagccgtc
     3721 gttacccatt tcgaagcgta cgccagcaaa agaaggcatt aaccgctggt agccaaaacc
     3781 cagcaacctg gcgctggcca cgatgcgcag gcgtactggc gttttttcgg ctgtacgctc
     3841 ttccaggcca aagctggctt gccagcgttt tttaccgctc aacaaatcac ttgcgatcgc
     3901 cgtggcttcg gtatcctgct gatcatcttt aaacaacata tccgtactgg aaaacaccag
     3961 catcttgtcc tgatatgtcg cgaacatcag ggcgttattg ccgttatagc gcaactgata
     4021 aacaggaata gtttcactat ttatcttgat gctactgatt tccgttttgc ttaactggct
     4081 gtcgctggta gcggcaaaca acaatggctc cagcagtttg cttaaaccac tgcgctggat
     4141 cagcaccatg taatgtgaaa gatggccttg tttatcgtgc catagcgccg cctgcgcggg
     4201 ctgatctaag agtgacgaaa agagcttatc tttcagcgtg agatcgtgtt catagacaat
     4261 gcggcgaatg ctgccttcaa tgcccagacg atctgcatga ttctgataat agaaaacgaa
     4321 atcctcgctc agaacatcgt ggagaaacgg aatggtgagg agatctttgg gaagctggct
     4381 cagagagtcg ctgtctagaa agaggtccgg ctcattgaga tcgatttgca gattgttgtg
     4441 caccaccagc ggcgacaacg ttttttctgg cccactgcca gcatattgca acgcccagac
     4501 gccagcggaa agcagtgcta ttgcgccaaa acctacaaga ccatagaacc gccagccttt
     4561 cgcctttttt tcaccactca ttgccacatt ccttgtgtat agccagccat tttttacggg
     4621 cacagccaaa ctttaccgcg ccctaatacg acaaaagccc agactttgca gcctggactt
     4681 ttcaattcaa acaagggaga tagctccctt ttggcatgaa gaagtaaaat tattcttctt
     4741 ctggctcgtc gtcaacgtcc acttccggag caatttcatc atccccttcc gcggcactgc
     4801 cgtcgatggt atccagatct tcctcgtcaa ccggttcagc aacacgctgc agacccacta
     4861 cgttttcatc ttccgcagta cggatgagga tcacgccctg ggtgttacgg cccacgatgc
     4921 tgatttctga aacgcgagta cgtaccagcg taccggcatc ggtgatcatc atgatctggt
     4981 cgcagtcatc tacctgcacc gcgccaacca ctaaaccgtt acgttcggta accttgatgg
     5041 agataacccc tttcgtcgca cgcgacttgg ttgggtattc cgccactgca gtacgtttac
     5101 cgtaaccgtt ttgcgttgcg gtgaggattg cgccatcgcc acgaggcacg atcagagaga
     5161 cgactttatc gccttcacct aagcgaatac cgcgcacacc agtggtattg cagcccatcg
     5221 cacggacaga agactcttta aagcgcacca ctttaccttc ggcggagaac agcattactt
     5281 catcttcgcc actggtcaga tcaacgccga tcagctcatc gccgtcaacc agtttgatcg
     5341 ccactttacc ggcggtacgc agacggttga attcggtgag gacggttttc ttcacagtac
     5401 cgttagcggt cgccatgaag actttcacgc cttcttcaaa ctcggtcacc ggcaggatcg
     5461 cggtgatacg ttcgtcctgt tccagcggta gcaggttgac gatcggacga ccgcgcgcgc
     5521 cacgagtggc ttccggcaac tggtaaactt tcatcgaata gacgcgacca cggctggaga
     5581 agcacagaat atggtcgtgc gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt
     5641 taatacgtgc ggcagattta cctttcccgc cacgacgctg cgcttcgtat tcagaaagcg
     5701 gctgatattt aacgtagccc tggtgagaga gcgtcacgac cacatcttcc tgggtgatca
     5761 gatcttccag gttgatgtct gcgctgttgg cggtgatttc agtacgacgt ttgtcaccga
     5821 actgttcacg aaccagctcc agctcttcgc ggatcacttc catcagacga tcggcgctac
     5881 caagaatacg caacagttcc gcgatctgat ccagcagctc tttgtattcg tcgagcagtt
     5941 tttcgtgctc aaggccggtc agtttctgca aacgcagatc cagaatcgcc tgagcttgct
     6001 gttcggtcag gtagtacaga ccatcacgca cgccgaactc tggctccagc cattccggac
     6061 gcgcagcatc gtcgccagca cgttccaaca tggcggcaac gttgcccagc tgccacggat
     6121 tagcaaccag cgcagttttc gcttctgcag gcgtcggcgc atgacggatc agttcgatga
     6181 tcgggtcaat gttcgccagc gccacggcta atgcttcaag gatatgagca cgatcgcgag
     6241 ctttacgcag ttcgaaaata gtacgacggg tcaccacttc acggcggtga cgaacaaacg
     6301 ccgcgatgat gtctttcagg ttcatgatct tcggctgacc atggtgcaat gccaccatgt
     6361 tgataccgaa agaaacctgc aactgggtct gggagtagag gttgttgagc acaacttcac
     6421 cgaccgcatc gcgtttcact tcaatcacga tgcgcatacc gtctttgtca gactcgtcac
     6481 gcagcgcgct gatgccttcc acgcgttttt cttttaccag ttccgcaatc ttctcgatca
     6541 ggcgcgcttt gtttacctga tacggaattt cgtggacgat aatggtttca cgtccggttt
     6601 tggcgtcaac ttccacttca gcacgggcgc ggatatatac cttgccgcga ccggtacggt
     6661 aagcttcttc aataccgcga cgaccgttaa tgattgccgc cgtcgggaag tccggccccg
     6721 ggatgtgttc catcagccct tcaatgctga tgtcttcatc atcgatatac gccagacaac
     6781 cgttgatgac ttccgtcagg ttgtgcggcg ggatgttggt tgccatacct acggcgatac
     6841 cggaagaacc gttcaccagc aggttaggaa ttttggttgg catgacgtcg ggaatttttt
     6901 ccgtgccgtc atagttatca acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt
     6961 catgggcaat tttcgccaga cggatttccg tataacgcat tgccgccgca gagtcgccgt
     7021 cgatggaacc gaagttaccc tgaccgtcta ccagcatgta acgcagcgag aatggctgcg
     7081 ccatacggac gatcgtgcca taaaccgcca agtcaccatg gggatggtat ttaccgatta
     7141 cgtcaccaac gacacgggca gattttttat aggctttgtt ccagtcattg cctagtacgt
     7201 tcatggcgta aagtacgcga cggtgtaccg gcttcaggcc atctcggaca tctggcagcg
     7261 cacggccaac aatgaccgac atcgcataat ccagatagga gctctttagc tcttcctcaa
     7321 tgttgaccgg tgtaatttct ctcgcaaggt cgctcatcta accgctatcc ctctactgta
     7381 tcccggattc aaaggtcgca aattataaca cagccgcgca gtttgaggta aacctatacg
     7441 ctttattcac atccaatgcc tgatatactc gtttgtcttg ccaattacgg agtagaagtg
     7501 ccaatgaatg ccgaaaaatc gccggaaaac cataacgtag accatgaaga gatcgctaaa
     7561 tttgaagccg tcgcctcccg ctggtgggat cttgaaggtg agttcaaacc gttgcaccgc
     7621 attaacccgc tgcgtctggg ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag
     7681 gtgctcgatg tcggttgtgg cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg
     7741 acggtgaccg gtctggatat gggctttgag ccattgcagg tggcaaagct gcacgcgctg
     7801 gaaagcggca ttcaggtgga ttacgtgcag gaaaccgtgg aagagcacgc ggcaaaacat
     7861 gccgggcagt atgatgtggt gacctgcatg gagatgctgg agcacgtacc cgatccgcag
     7921 tcagtggtca gagcctgtgc gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg
     7981 cttaaccgca acggcaagtc atggctgatg gcggtggttg gcgcggaata tattttgcgc
     8041 atggtgccca aaggtacgca tgatgtgaag aagtttatta aaccggcaga gctgcttggc
     8101 tgggtggatc agaccagttt aaaagagcga catataaccg ggctacatta caacccgatc
     8161 actaattcct ttaagctcgg ccccggcgtg gatgtgaact atatgctgca cacgcagaat
     8221 aagtgaggtt gatgtttggc cgcgccaatg cctgatgcga cgcttgcagc gtcttatcag
     8281 gcctacaaat gctctccgta ggccagataa ggcgtttacg ccgcatccgg caattgtgat
     8341 ggtcagacag cgattaccat ttcaccgtca tcgacaaaaa ccctgccgtc tgggcaaaat
     8401 catcactccc tttctgccac gccacgctgc cgcgcagaga cactcgctga ctgatattgc
     8461 ccgtgactcc cacttttatt tcaccccgta gcttcaccgc atcgtcactg atagtgctgc
     8521 catcttcttc aatttccgtc gcatggggat cgtgataata attcagatct aatgttggtg
     8581 tgacaccaac cgcggtacgc cattcgctgt gtaaacccag ccgcgtctga atatcatccc
     8641 cctgcgattg tgacacgcgc gcatggttag cggcggtaaa atcatcctgc tgcacgccct
     8701 gataaatcac ctgcgcctgc ggttcaatca ccacaccacg ccccggtaac cactgatacc
     8761 cggcttccag cgaggcgata atccccgacg aatggtaacg atccacgcca tcttcatgtt
     8821 cggaaacatc attgttaaac cacgcgtatt gcagccagct atccagccag gccccctgct
     8881 tctgattacc gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt
     8941 tatcggcgcg agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac
     9001 caaccgcccc aagcatccac tcgccatccg tgccccagca cccgctaaac aagccgccgc
     9061 taagcagcac cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat
     9121 aacgtccgcc gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc
     9181 gctccatcat aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg
     9241 cattcagcac cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg
     9301 gcgtgggatc aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt
     9361 gcgatcgcag ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt
     9421 tgacgtagcc actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag
     9481 ctgcgaaatc aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt
     9541 taaccaccac agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg
     9601 agtcatcgcc gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt
     9661 ttaccgtcag cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg
     9721 tcagatcacc attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg
     9781 tattaagctc gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt
     9841 taaatagtga ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac
     9901 catcgctgat gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg
     9961 cgccagtaac gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat
    10021 ccgcaacatc gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc
    10081 tggcggtcag ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat
    10141 gctgcccgct gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg
    10201 ccacttcacc gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag
    10261 tatcgacatt ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct
    10321 ggcggttata gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg
    10381 cgatttgtgt tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg
    10441 catcgaccac cacgtagacg ccatctttcc cgtcaccgcc gatatgcaaa ttgtcgccac
    10501 ctgcaagatc taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa
    10561 ggccatcatt gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac
    10621 cgccgtttaa cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc
    10681 cttccagcga aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact
    10741 gcgcccctgc cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga
    10801 tgatgccagc aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag
    10861 tgccattctg aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta
    10921 gctcagcctg attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg
    10981 gatcgtcctg gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa
    11041 cttcaccgtt ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca
    11101 gatcgccgga acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt
    11161 cattctctgt attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac
    11221 ttaagccgag atacataaag ccacccgccg cagtggaagg accatctcca tagcctgctg
    11281 cgctattgtt ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa
    11341 tatcaataag atagggagcc ctcacgctat tggtatatat cgccccgcca taaccttcgg
    11401 ctgtgttatt tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt
    11461 cgctattatt atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg
    11521 tatatgcctg gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat
    11581 aaattgcgcc acctttgcca tcattagcga tattattgcg aaacatggcg ttagtgacac
    11641 gtaaatcgac ggcaccagta tcattagtac cagaagaata gattgcgcca ctatagccgc
    11701 ctgcgacgtt accggaaaaa ataacatcag taagattcag cgttgagttt tctttagcaa
    11761 atatggcccc gccgttatta tattctcctg taacggtgtt attggcaaac agagtcattc
    11821 cagtttcatt ttctggtaat agtgaaaatt ccgctccttg ttgcaaaaat acggccccac
    11881 cgctggcatt attggtcata tccgaaaaaa ccagccattg cccatcgg
//
LOCUS       DAJTXY010000116        11884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTXY010000116 DAJTXY010000000
VERSION     DAJTXY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.116"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(69..236)
                     /locus_tag="OO566_003287"
     CDS             complement(69..236)
                     /locus_tag="OO566_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606460.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHHAA"
     gene            complement(424..1263)
                     /locus_tag="OO566_003288"
     CDS             complement(424..1263)
                     /locus_tag="OO566_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6606461.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVAYIFKHPAGKLFWALTDESNKEQADVIPVYAAAPASVVPDNASEPLAYAYKELT
                     PEIMRNHLAVFERYGIAPNDSSTTIQALRIALDGIERSDAMLHGAEPVSQTYKLPPLS
                     SNEVNDAAWKLHNMLTEHGPLNGRQFNNLKGCFYEALKVAMRNYPVTPDGWISCSERM
                     PEDTKMLLAFSQGQIVAAYWNWVMSPIDYKKYRAFTYLSGNILDDVTHWMPLLEPPEQ
                     DGE"
     gene            complement(1265..1951)
                     /locus_tag="OO566_003289"
     CDS             complement(1265..1951)
                     /locus_tag="OO566_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6606462.1"
                     /translation="MSEINYQALRKAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVAQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEGKDSKIANLTAERDALREGEMGDARYSNTRAAADIYFQLVEECEIPAGGSLV
                     EYVDDMREKLESAEKRIAELEEREILLPERSSMLHRTDFHEDYQTVMAYKVSEVIDAI
                     RATGIRIKGE"
     gene            complement(1938..2252)
                     /locus_tag="OO566_003290"
     CDS             complement(1938..2252)
                     /locus_tag="OO566_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606463.1"
                     /translation="MTITKQRVEKIIYRHEMGLSSDVTAEEVYDLAVLALNLSNIANL
                     KRYELDMGGCDSCGQDCGADMTEDSDGDYVLFDDVVKLFEFDTTTQKLESPAKEATSE
                     RN"
     gene            complement(2249..2734)
                     /locus_tag="OO566_003291"
     CDS             complement(2249..2734)
                     /locus_tag="OO566_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606464.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVNETNAEYLVRKFAEAEAKCA
                     ALAAENAGLKAALNHEVIPDTAVEAFNETAIIDHDWNDTSEWSWVENDTDVIRAVLEA
                     IKPETPATDAFLAEIERKAIRKFINSIEHILRDKLSPYDTEEMLETMRIFLEEQGGEQ
                     K"
     gene            complement(2731..2895)
                     /locus_tag="OO566_003292"
     CDS             complement(2731..2895)
                     /locus_tag="OO566_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCR6606465.1"
                     /translation="MRGLAYNLGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRNGKK"
     gene            complement(2906..3199)
                     /locus_tag="OO566_003293"
     CDS             complement(2906..3199)
                     /locus_tag="OO566_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCR6606466.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3213..3719)
                     /gene="ssb"
                     /locus_tag="OO566_003294"
     CDS             complement(3213..3719)
                     /gene="ssb"
                     /locus_tag="OO566_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6606467.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(3720..4427)
                     /locus_tag="OO566_003295"
     CDS             complement(3720..4427)
                     /locus_tag="OO566_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR6606468.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIRIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKHGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(4682..4834)
                     /gene="kil"
                     /locus_tag="OO566_003296"
     CDS             complement(4682..4834)
                     /gene="kil"
                     /locus_tag="OO566_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCR6606469.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(4819..4950)
                     /locus_tag="OO566_003297"
     CDS             complement(4819..4950)
                     /locus_tag="OO566_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HCR6606470.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVDVLLCAMK"
     gene            complement(4975..5943)
                     /locus_tag="OO566_003298"
     CDS             complement(4975..5943)
                     /locus_tag="OO566_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HCR6606471.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVHDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAKRERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(6111..6353)
                     /locus_tag="OO566_003299"
     CDS             complement(6111..6353)
                     /locus_tag="OO566_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606472.1"
                     /translation="MRVKTMGASLLSGRIFQGTLNTEKGMWVGKREDVTDQAVRAVAE
                     HMMIKDQKYAYETKDGKWLIISHQLVDKLPEEFVDG"
     gene            complement(6556..6888)
                     /locus_tag="OO566_003300"
     CDS             complement(6556..6888)
                     /locus_tag="OO566_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6606473.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRTERKNKRTWYSKPRSEIGVTCSGRQKQRG
                     KSIPAYYD"
     gene            complement(7349..7753)
                     /locus_tag="OO566_003301"
     CDS             complement(7349..7753)
                     /locus_tag="OO566_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606474.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGSMNGDRYEFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            complement(7750..8382)
                     /locus_tag="OO566_003302"
     CDS             complement(7750..8382)
                     /locus_tag="OO566_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR6606475.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            8486..8701
                     /locus_tag="OO566_003303"
     CDS             8486..8701
                     /locus_tag="OO566_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6606476.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            8821..9114
                     /locus_tag="OO566_003304"
     CDS             8821..9114
                     /locus_tag="OO566_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HCR6606477.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            9147..9293
                     /locus_tag="OO566_003305"
     CDS             9147..9293
                     /locus_tag="OO566_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HCR6606478.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            9286..10119
                     /locus_tag="OO566_003306"
     CDS             9286..10119
                     /locus_tag="OO566_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HCR6606479.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTDKEYNNIISNTDVLESTAA
                     DKKSDKKKPSVSCQDVVDAYHEILPEAPKIRALNDKRKNQIRTFWRKAGVITRQLDGH
                     GFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGFDFLLNDNTYLKVREGEHDD
                     R"
     gene            10109..11545
                     /locus_tag="OO566_003307"
     CDS             10109..11545
                     /locus_tag="OO566_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6606480.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPSETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            11622..>11884
                     /locus_tag="OO566_003308"
     CDS             11622..>11884
                     /locus_tag="OO566_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR6606481.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVP"
BASE COUNT         2867 a         2926 c         2626 g         3465 t
ORIGIN      
        1 agtcgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc tccaataaaa
       61 aaccgccatc aggcggcatg gtgttctttc agttcctcaa ttcgaatatt ggttacgtct
      121 gcatgtgcta tctgcgccca tatcatccag tggttatagc agtcgttgat gtcctctgct
      181 tcgataaccc tgtcgaatgg ctctccattc cattcacctg tgactcggaa gtgcatttat
      241 catctccata aaacaaaacc cgccgtagcg agttcagata aaagaaatcc ccgcgagtgc
      301 gaggattgtt agttgctatc tgctgcttcc ttggtcatta ccatatccac ccaatttcac
      361 cagacatgat tctcgcaatc actattatca ccaaagtgat aatcacaact ttaactggcg
      421 gcatcattca ccatcctgct ccggcggttc tagcagcggc atccagtggg ttacgtcatc
      481 caagatattt cctgataaat atgtgaaagc tctatatttt ttgtaatcaa ttggactcat
      541 tacccagttc caatatgctg ccactatttg accttgacta aatgccagta acattttggt
      601 gtcttccggc attcgctcac tacagcttat ccaaccatcc ggagttaccg gatagttgcg
      661 cattgcgacc tttaatgcct catagaagca acctttcaga ttgttgaact gacgcccatt
      721 tagcgggcca tgttcagtca gcatgttgtg taatttccat gccgcgtcgt ttacttcgtt
      781 ggatgacagg ggaggcaact tgtaagtttg gcttacaggt tcggcaccat gaagcatggc
      841 gtcgctccgc tctatgccat ccagcgcgat tcgtagtgcc tgaattgtgg tagagctatc
      901 gtttggggct attccatatc gctcgaatac agctaaatgg ttgcgcataa tctcaggcgt
      961 aagctctttg taagcataag cgagaggctc tgatgcatta tccggcacaa ccgacgcagg
     1021 cgcggcagca taaacaggaa taacgtccgc ttgctcttta ttgctttcat ccgttaaagc
     1081 ccagaataat ttcccggccg gatgtttgaa aatataagca actggctctg cttccagcga
     1141 tgccagtgca attcgtgcca gttcttccgc ttcttctgct ggcagtacaa cgttgctacc
     1201 aggtccgtat gtttcgcgcc actgcttgat tgtcagtagt cgctctttgg taatagtggt
     1261 catctcactc tcctttgatg cgaataccag tagcgcggat tgcatcgatg acttcagaaa
     1321 ctttgtatgc cattaccgtt tggtaatcct cgtgaaaatc tgttcgatga agcatgctgc
     1381 tacgttccgg gagcagtatt tcccgctcct ccagttctgc tatgcgcttc tctgcggatt
     1441 ccagcttctc gcgcatatcg tcaacgtact cgaccagaga accgccagca ggaatttcgc
     1501 actcctcgac cagttggaag tagatatcag ctgcggcccg tgtgttgcta tacctagcgt
     1561 cgcccatctc accttcacga agagcatcgc gttcggcggt aagattggct attttgctgt
     1621 ctttgccttc cagctcaacg cgcagcttcc ctaccgtaag cgcaatatcc tcgttctcct
     1681 ggtcgcggcg tttgatgtat tgctggttcc tttcccgttc atccagtagt gcctgcgcta
     1741 cttcagggtt gaaagctgcg atataacgag cgttgttctc tgcgttgttc tgtccatcaa
     1801 agccggtcca tttgataacg tcttcacatc gtttatcacc aggtgtatgc accgcatatg
     1861 taccagtacc agacgaaata aatgcgaccc attcaccctg tgttgccagt tgcgccgctt
     1921 tacgcagtgc ctggtaatta atttcgctca ctggttgcct cctttgctgg gctttctaac
     1981 ttttgagtgg ttgtatcaaa ttcaaacaac ttaaccacgt catcaaacag gacataatca
     2041 ccatcagaat cttcagtcat gtcagcgcca caatcctgac cgcatgagtc acaaccaccc
     2101 atatcaagct cgtatcgctt caggtttgcg atatttgata aattcagcgc cagtacagcc
     2161 aggtcataaa cctcttcggc agtgacatcg ctgctcagtc ccatttcatg gcgatatatg
     2221 attttttcta ctcgttgttt tgtgattgtc atttttgctc gcctccctgt tcttccagaa
     2281 aaatacgcat agtctcaagc atctcttcgg tgtcatacgg tgacaacttg tcacgcagga
     2341 tgtgttcaat gctgttaatg aacttgcgga ttgctttgcg ttcaatttca gccagaaaag
     2401 catcggttgc cggggtttca ggtttaatgg cttcaagcac ggcgcgaatg acatcggtat
     2461 cgttctccac ccatgaccat tcgcttgtat cattccagtc gtggtctatg atagctgttt
     2521 cattgaatgc ctcgactgcc gtatccggga ttacttcatg atttaaagct gccttcagcc
     2581 ccgcattctc cgccgccagc gccgcgcact tggcctccgc ttcagcaaat ttacgcacca
     2641 gatattcagc gtttgtttcg ttaaccttta aatcacttgg gatgcattta cctttcagaa
     2701 atccatccat ctcaattagt gtcatttgtt tcatttcttc ccattccgcc acattgcatt
     2761 cagatatttg ttttgattta ctgacggaaa agaatttctt ttaagcaatt cctctctcga
     2821 tggcattggc tttacgcgtt ggcgaataat catttctgcc ggaagaatgc cgagattgta
     2881 tgcaagtcct ctcatggtaa attcctcagt cattactgat agcgccatag cgtgagcggt
     2941 aattacgcag gcgcgggtca atttcaggga agtgggtata tgtggctttg cggaatggtc
     3001 ggattgatgt ctggtaaatt cgctcgcgct cttctttctc tgcaagccat atgcagtggc
     3061 gaaattcctt ttcctctttc gtttcctgcg gtagcgacat tatccggtcg tagtttttcc
     3121 tgaatttatc cagcacctcc gatacggaat tgccggaaca gcggcgcggg tcatccgcac
     3181 catactgagg cgctggcatg attttctcct gattaaattg cgtgaatagc gtgacgaggg
     3241 aaggggagag ttactggtgc aaagggtata tcgtcgtcaa aatccatcgg aggttcgttg
     3301 tgttgtgctg gtgatgattg ctgctgtggc ttctgtgatt gcctgctggc tgcttgttgt
     3361 ttgctgtcgc caatgccgcc aagcatttgc atcacgccat taattccgac atgaacctcg
     3421 gttgtgtaac ggtcttgccc tgactggtct ttccactttc tggttctcag cattccctcg
     3481 aaataaatct gatcaccttt tttcacatac tgccccacga cctcagccag tttcccggat
     3541 acagcaacac gatgccattc agtcaattcc ttttgctcgc cagtattttt atctcgccat
     3601 tgttctgacg tggctattgt caggttagcg aacgctgttc ctgatggtga gtatcgaact
     3661 tccgggtctt gtcctacccg accaaggata atcaccttat ttacgcctct gcttgccatt
     3721 tatgccgcct gttttagttc gttaactctg atgttcatta cctgaacgca tttagcctgc
     3781 gcttcctcgt tgccagccat taattgccag tcacgctgat aacgctcgat gagttttttc
     3841 ttgtcagttt ctgttgacgc ataatcgctg aagtctttca ggatttgttc gcagtcaacc
     3901 gatggagatt tctggttggt attttctggt gatggtttgt tatctgatgc tgggattgcc
     3961 catcccggca gcgatggagg gagccagtaa aatcctgttc catccttcag ttttgccctg
     4021 tgccacccat gctttttatc gagagatgtt tgtgcgaaac cttcctcaag gttatacaga
     4081 taccgaccga ttccccactg aacggcagca cgcttcattg cacctgaacg accacctttg
     4141 acggcttcta cctgcgtgtt ttcagcagca tcccatttgg ttacccattc ggaatcaatc
     4201 ctgattgata tgccgcattc aacgcctccg ttgttgggaa tatcgcggta ttcattgcgc
     4261 catcctgctt tgccgcaaac atcgtccagg cgtttcatga ttgcccggtt cgtgacataa
     4321 gccagcacca tagcccacac tttgccatcg cgtgttttac cgctttgctg tattcgccat
     4381 tcgatatctt cagggctgaa tggctcatcg aatttattca aatccataaa tcacctcaga
     4441 atggacatgg cccaagaaaa taacgctgat ttaatacttc gactcgggac aaattaaggc
     4501 atacccgcat tccttcgcgg tcgccattat ggcgatacca gagagctttc tgggtgtaca
     4561 tgcgtctctg taacttgctc tccttcactg tggttgcaag tgacatgaat atctccttcg
     4621 tttccgatta attctttcat ctgacgaatg aattcttcgt ctgaccagtt atctgtaaaa
     4681 ctcatttcct gcgataccac ggaaggttga tagctgactt catcgcttta tttgcttcaa
     4741 gccacatttt ggaatcacca ataaatctgg ctattactgc tttgttctgt gcagcacgaa
     4801 gcatctggtg attgatggct atttcattgc gcataataag acatcaactc ttttccatcc
     4861 gtcacgtaat ttacgggtga ttcgttcaag taaagattca tttaattgga aggcacccat
     4921 gcgagcgcct cccgcgattg cgtaaatcat gggtggttcc ttatgttggt tttattagta
     4981 ggttattttt gttgcgaata cttcgccttt tacgatggct gttatgatat ttttagcaac
     5041 atcttctgat gcgccaacct tgataaggtc agcaagtatt ttgttattta cttcttttcg
     5101 gtgagcttta tcctttgctc tacgctcttc ttcgtccttg attctttttt cttctgctat
     5161 tctggcttgc tcttttgctt cagcttcgcg ccggattcgt tcagcctcct cctgtgcttt
     5221 tcggcgttct gcttcaattg ccgcctgctt ttctctttca gctcgttctg ctgcctcttt
     5281 tgcttcgcgc tgtgctcgtt gctcggcttc aatgcgttca cgctttgcac gttccgctgc
     5341 ggccttagct tctgcttctc gccttgctgc tgcttcaatt tcggcttttg cctttgcttc
     5401 ggcttctgct ctggctttct cttcagcttc tctttttaag cgttcttcat gctctcgctt
     5461 ttcctgctcc gctttgagtc ttgcctcttc tctttggcgg tcaaattcgc gatccatcaa
     5521 aatcgctatt tcatggtcag actcaatttg ctttgcgaga gcttcagctg ctgccttagc
     5581 ttcttcttcg gctttaatcc gcgcctgttc ctcctcataa tcagtaagag gctggcgcgc
     5641 cttggctttc agctcatcaa gtcgatcatg cactgttttg cggttggcat caattagctt
     5701 tggaatttcc ttcagttcag caacaaggtc tttgccaaga ccatcgagat atgttttcgt
     5761 ctgcgcaact ttatacgcca gagaagcgat ctcctttctg ccctttgccg ttgtgatatc
     5821 aggcacaaag gacataactt cacgttcaac cttttgaagg atttcttcaa tctggtcggc
     5881 agactgaaat acagtcattg catttgcttt ttcaataaca actaaatctg ttacttcact
     5941 catatttccc ccgtcaaaaa aattgccctc acactggagg gcaaagaaga tttccaataa
     6001 tcagaacaag tcggctcctg tttagttacg agcgacattg ctccgtgtat tcactcgttg
     6061 gaatgaatac acagtgcagt gtttattctg ttgcttattc caaagcaaat ttaaccatca
     6121 acaaactctt ctggtaattt atcaaccagt tgatgactta ttatcagcca tttgccatcc
     6181 ttcgtttcgt atgcatattt ctggtctttt atcatcatgt gttcggctac tgccctcact
     6241 gcctgatcgg tgacatcttc tctctttcct acccacattc ctttttcagt gtttaatgtt
     6301 ccttgaaaaa tacgaccact taataggctt gcgcccatag tttttactct catgtatcag
     6361 tcctcaaata agtggtttgc tgcgaaagta aatacgctta agttacctgt tatttatccc
     6421 accaagttcc gtatctatct atccagttac accaatcatc gacactctat tttgttgtgt
     6481 cgcattttgg caactggcaa gaatatctac cttctttgta aagtcggcgt ttgactttct
     6541 tgagcatggc tcacctcaat cgtaataagc tggaattgat tttccgcgtt gcttctggcg
     6601 gcctgagcaa gtcacaccta tttcactgcg tggcttgctg taccatgtgc gcttattctt
     6661 gcgctcagta cgctgcaggt tgctttcaat ctgttcgtgg tattcagcca gcaccgtaag
     6721 gtctatcgga ttcagtgcgc tttctactcg tgatttcggt ttgcgattca gcgagagaat
     6781 agggcggtta actggttttg cgcttacccc aactaacagg ggatttgctg ctttccattg
     6841 agcctgtttc tctgcgcgac gttcgcggcg gcgtgtttgt gcatccatct ggattctcct
     6901 gtcagttagc tttggtggtg tggtggctgg tagtctagct ccagcttgtt gagtctcatt
     6961 cggaggggta taaccggcac cccagcgatt tttccatgcg acaacgtgcg cgttatggcg
     7021 gccttatcgc ccgcggctcc ccatctcgtc cacgctattg ctagcgttgg gagcgcttca
     7081 ccgctcaaca gtaggtaagc acttgccagt gactagctgg cttcaccaca ccccaaagcc
     7141 ttctgctttg aatgctgccc ttcttcaggg cttatttttt aagagcctca ccttcatggt
     7201 ggtcagtgcg tcctgctgat ggcttaaaat tacaagaaag attgtatgtt gtaaacaaga
     7261 aatattgtga aaaggggcgt gaaaaacaaa ctccattgtt tttaaatgaa aaatagtttg
     7321 tttttggtta tcgagattga ggtggggatt actggttgca ggttccgact acatcaccaa
     7381 caaatgattt ggttgatgta agttgttgca tacctgggat attcattact ttggagtaaa
     7441 gagctttttt gtctgtagtg attgaccaag tttcaacggt tattcctcct ccagactggt
     7501 attctcctac catagtgttc gatgacaaag cagtgtattt catctctgga tagacgccag
     7561 aaactgattc ataaactgat gatttatcgc catttattgt tacgtggaaa acggaatctt
     7621 ccgtgctgtc ttttgtaaac tcgtaacgat cgccattcat tgacccgtac ccgtgcaggt
     7681 ttgtgacaat ccagcattca gaattggcgc tggtagttaa gagtattgag agtagcgccg
     7741 caatcctgat catacgaatt ttaccctcgc ttccacgaca acaccgataa tcttgcagtt
     7801 cccgttgata ggagtcatag gccatgaagg gttcaggcct ttcaggtact tctgcccgcc
     7861 atctataacc agtttcttga atgttgcttc gttcgcgtca gtcagtttgg ctacaacaag
     7921 gcttccattc actggctcgc gtccagtatc tactaacacc atatgacctt cagggatgct
     7981 ttgacctaca ggtgaggtca tggaatcacc ttcaaccttc agccagaatc catcgcctaa
     8041 taagttaacg tcactgtcat accattcatc aatgtccttg atatcgtagg gttcacaagc
     8101 ttcacaccac gaaccagctc taaccatgct aatcaatgga tatttccctt tgggttcaac
     8161 atgcccaaca aatctaacat tcgaatcaga ggtgccattg agcagccagt caacacttac
     8221 gccaagagct gacgcaagtt ctggtaaaaa gcgtggtcgc ttagttttac cgttttcgag
     8281 ctgctctata gactgctggg tagtccccac cttttgagca agttcagcct ggttaagtcc
     8341 aagctgaatt cttttgcttt ttaccctgga agaaatactc ataagccacc tctgttattt
     8401 acctccaatc ttcacaagaa aaactgtatt ttacaaacaa gatacattgt atgaaaatac
     8461 aagaaagttt gttgatggag gcgatatgca aactctttct gaacgcctca agaagaggcg
     8521 aattgcgtta aaaatgacgc aaaccgaact ggcaaccaaa gccggtgtta aacagcaatc
     8581 aattcaactg attgaagctg gagtaaccaa gcgaccgcgc ttcttgtttg agattgctat
     8641 ggcgcttaac tgtgatccgg tttggttaca gtacggaact aaacgcggaa aagccgctta
     8701 agacattccc gctcttacac atcccagtcc tgaaaaaggg catcaaatta aaccacacct
     8761 atggtgtatg catttatttg cataccttca atcaattgtt atctaaggaa atacttacat
     8821 atggttcgtg caaacaaacg caacgaggct ctacgaatcg agagtgcgtt gcttaacaaa
     8881 atcgcaatgc ttggaactga gaagacagcg gaagctgtgg gagttgataa gtcgcagatc
     8941 agcaggtgga agagggactg gattccaaag ttctcaatgc tgcttgctgt tcttgaatgg
     9001 ggtgtcgttg acgacgacat ggctcgattg gcacgacaag ttgcttcgat tctcaccaat
     9061 aaaaaacgcc cggctgcaac cgagcgttct gaacaaatcc agatggaatt ctgaggtcat
     9121 tactggatca atccacagga gtaattatga caaaacaact cagtccttac caggacaaaa
     9181 ttcacaaaca catactacgt gatcgcttcc tgtccagctt caagcagcct ggtcgattcc
     9241 gggctgagtt ggaaaaagtg aagctgatgc agaaggagaa aggtcatgag taacatatct
     9301 aatctagccg aagccagaga ggccagaagg ctacaacaac cgcatcaaag cagcggtaag
     9361 gggtatgcct tgctgcaccg taaaattatg gatgtgccgt tttacaagga cgcagaagca
     9421 gcgcatctgt gggttcactt aatcctcaaa gcaaagcata cgcctgagta tgtaatgact
     9481 gacgcaggag aaattctggt aggcagaggg aagctacttg gcggtagaaa ctctctggcg
     9541 tttgaaacag gactcaaacc agatcgcgtt cagtacctgc ttagaaagtt caaaaaactc
     9601 ggcatgattg actgggtttc acacggtaaa ttctcagttt tctcggtaga gaaatatgac
     9661 gattatcagt caaattttgt accagcagat taccagcaaa ttaccacaga taaagaatat
     9721 aataatatta tctctaatac tgacgtatta gagagtaccg cagcagacaa aaagtctgac
     9781 aagaaaaaac cttccgttag ctgtcaggat gttgtcgatg cttaccacga aatccttcct
     9841 gaagcgccaa aaatccgcgc actgaatgac aagcgtaaaa accagatccg aacgttctgg
     9901 cgcaaagccg gagtgataac ccgccagctt gacgggcatg ggttcacgat gcaggactgg
     9961 agaaattatt tgagctacgt aggcgaaaat tgccgatgga tgttcgaaga gcgcccaaac
    10021 catcagcgcg gaaccgtctg gcacaaaaag ggatttgatt tcctgcttaa cgataatacc
    10081 tacctgaaag ttcgtgaggg tgaacacgat gaccgataat ttttatgcgc cgccccatag
    10141 catcgaggca gagcaggcgg tgattggtgg attgcttctg gatgatgaca gcagtgagcg
    10201 cgtccagaag gttctggcga tgctgaagcc tgattcattt tacagccgac cacacaaaat
    10261 cattttcgaa gaaataacca gaatgcaccg ggagcaaaag ccagtagatg gcctgacgct
    10321 tttcgatgaa ctggagcgta aatcgttaac ggcgtctgtt ggcggttttg cttatatcgc
    10381 tgagatcgca aagaacacgc caagcgccgc aaacatcgtt gcctatgcaa tgcaggttcg
    10441 cgaaaccgca atggaacgct acgccatcaa ccgcatgact gaagcgacgg aattgctcta
    10501 ttcccgcaac ggaatgactg cgacgcagaa gtacgaagct attcaggcga ttttcacgca
    10561 actgacagac catgcaaaaa caggatcgcg tcgcggcctt cgctcatttg gtgaggtcat
    10621 ggaagactgg gttagcgacc ttgagaagcg atttgaccca tcaggcgaac aacggggaat
    10681 gagcacaggg atcccatcgc tggacaggat gctgtcaccg aaaggtctgg tgaaaggctc
    10741 tctgtttgtc attggcgctc gacctaagat ggggaaaacg acgctataca gccagatggc
    10801 aatcaactgc gcagtgcatg agaaaaagcc cgctcttatg ttcagccttg aaatgccagg
    10861 tgaccagata ctggaaaaac tggtagggca gaagtctggt gttaacccga atatttttta
    10921 ccttccggcg acaaatgacg ctgatgacgg ctatcagggt gattacgatg gtgacttcaa
    10981 cagggcgatc gaaacagcca atcgcttgag tgaaatcgac ctgctttaca tcgacgacac
    11041 gccgggatta tctctggctc aaatcgtcag cgaaagccgt cgaatcaagc gagaaaaagg
    11101 atgtgttggc atgattctgg tcgattacct gacactaatg accgctgaaa aggccgatcg
    11161 caacgacctt gcttacggca tgatcaccaa aggactgaag aaccttgcca aagagcttga
    11221 ttgcgttgtt gtgcttctga cacagcttaa ccgcgcactg gaaagccgaa ccaataaacg
    11281 cccattacca agtgactcac gagatacagg gcagattgaa caggattgcg attattgggt
    11341 ggggatccat cgtgaaggtg cttttgatga cagtgttcca cctagtgaaa ccgaactaat
    11401 ccttcgtctc aatcgtcatg gcaataccgg cacggtgtat tgcattcagg caaatggcgc
    11461 tatttatgac acagaccaac agtctgctga aatgcgccga cgtgaacgcg aggaaccgca
    11521 gtccaagaag aaaggaggat tctgatgacc atctacatca ctgagctaat aacaggtctg
    11581 ctggtaatcg caggcctttt tatttggggg agagggaagt catgaaaaag ctaacctttg
    11641 aaattcgatc tccagcacat cagcaaaacg ctattcacgc agtacagcaa atccttccag
    11701 acccaaccaa accaatcgta gtaaccattc aggaacgcaa ccgcagctta gaccagaatc
    11761 gaaagctttg ggcttgcctt ggtgacgtct ctcgtcaggt tgaatggcat ggtcgctggc
    11821 tggatgcaga aagctggaag tgtgtgttta ccgcagcctt aaagcagcag gacgttgttc
    11881 ctaa
//
LOCUS       DAJTXY010000117        11837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTXY010000117 DAJTXY010000000
VERSION     DAJTXY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.117"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..1009
                     /locus_tag="OO566_003309"
     CDS             <1..1009
                     /locus_tag="OO566_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6606482.1"
                     /translation="EKWLDLKRMEISANAFNRYESVARTMVPKIGGGRLVSSVTKEEL
                     LYIRKDLLTGYQNSTKNKAAAKGRSVVTVNYYMTTIAGMFQFAADHGYLEANPFQGIK
                     PLKRARAEPDPLTRDEFIRLIDACRHQQTKNLWSLAVYTGMRHGELVSLAWEDIDMKA
                     GTITIRRNYTKLGEFTLPKTEASTNRVVHLIQPAISVLKNQAEMTRLGKQHHIKVQLR
                     EYGRSVNHECTFVFNPQVVRKSKQVGFIYKVDSIGDSWETAIKRAGIRHRKAYQSRHT
                     YACWSLSAGANPSFIASQMGHASAQMVFNVYGAWMTDSNAEQIAMLNQKLADYVPWSP
                     K"
     gene            1022..1792
                     /locus_tag="OO566_003310"
     CDS             1022..1792
                     /locus_tag="OO566_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6606483.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            1845..2240
                     /locus_tag="OO566_003311"
     CDS             1845..2240
                     /locus_tag="OO566_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6606484.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2281..3024
                     /gene="cmoA"
                     /locus_tag="OO566_003312"
     CDS             2281..3024
                     /gene="cmoA"
                     /locus_tag="OO566_003312"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6606485.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3021..3992
                     /gene="cmoB"
                     /locus_tag="OO566_003313"
     CDS             3021..3992
                     /gene="cmoB"
                     /locus_tag="OO566_003313"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6606486.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4157..6586)
                     /gene="torZ"
                     /locus_tag="OO566_003314"
     CDS             complement(4157..6586)
                     /gene="torZ"
                     /locus_tag="OO566_003314"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6606487.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMAAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6611..7711)
                     /locus_tag="OO566_003315"
     CDS             complement(6611..7711)
                     /locus_tag="OO566_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6606488.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8099..8845)
                     /gene="cutC"
                     /locus_tag="OO566_003316"
     CDS             complement(8099..8845)
                     /gene="cutC"
                     /locus_tag="OO566_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6606489.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(8859..9425)
                     /locus_tag="OO566_003317"
     CDS             complement(8859..9425)
                     /locus_tag="OO566_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6606490.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9641..11374
                     /gene="argS"
                     /locus_tag="OO566_003318"
     CDS             9641..11374
                     /gene="argS"
                     /locus_tag="OO566_003318"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6606491.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11551..>11837
                     /locus_tag="OO566_003319"
     CDS             11551..>11837
                     /locus_tag="OO566_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HCR6606492.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLK"
BASE COUNT         2935 a         3004 c         2863 g         3035 t
ORIGIN      
        1 agaaaagtgg ctggatctga aacgaatgga aatctctgca aatgcattca atcgctatga
       61 atccgttgca agaacgatgg ttccgaaaat tggaggcggc agactggtgt catcggtaac
      121 caaagaggaa ttgctgtata tcaggaaaga tttgctgacc gggtatcaga attcaacgaa
      181 aaacaaagca gcagcaaaag gacggagcgt cgttactgta aattattaca tgacgacaat
      241 cgctggaatg tttcagtttg ctgcagatca cggttactta gaagcaaatc ccttccaggg
      301 aattaagcct cttaaaagag ccagggcaga gccagatccg ttaactcgtg acgaatttat
      361 tcgcctgata gatgcttgcc gacatcagca gacgaaaaac ctgtggtcat tggctgtgta
      421 cacaggaatg cgtcacggtg aactggtctc cctggcctgg gaagatatcg atatgaaagc
      481 aggaactatt actatcaggc gcaattatac gaaactcggt gagttcactc tacctaaaac
      541 tgaagcaagt acaaacaggg ttgtgcatct tatccagccc gctatcagtg tcctgaaaaa
      601 tcaggctgaa atgacaagac tgggtaagca gcaccacatc aaggttcaac tacgtgaata
      661 tggacgttca gtgaatcatg aatgtacttt cgtatttaac ccccaggtgg ttagaaaaag
      721 caaacaggta ggttttatct acaaggtaga ttccattggc gactcatggg aaacagccat
      781 taagcgtgcg ggcatcaggc accggaaagc ataccagtca cgacacactt atgcgtgctg
      841 gtcattatct gccggagcaa acccaagctt cattgccagc cagatgggcc atgcaagtgc
      901 ccagatggtg ttcaatgtat acggagcatg gatgactgac agtaatgcag aacagatcgc
      961 aatgctgaat cagaagctgg cagattatgt cccatggtca ccaaagtgac acaagagact
     1021 tattaaaatc agtgggttag ccttcaatac ccgtcatgtt aactgcgtgg agggcaacac
     1081 cacgctttac gccctgccga aacccgaggt tgtcctgcgc tggcgtgaac agaccacaga
     1141 tgacttccgc ttctgtttta agtttccggc gaccatttcg catcaggcag cattacgaca
     1201 ttgcgatgat ttagtgactg aatttttgac ccgcatgtca ccgttggctc cgcgcattgg
     1261 gcaatactgg ctgcaactgc ctgccacatt cggcccacgg gagctgcctg cgctttggca
     1321 ttttctcgat tctctccctg gcgaatttaa ttatggtgtg gaagtccgcc atccacagtt
     1381 tttcgccaaa ggggaagagg aacaaacgct taatcgcggt ttacatcagc gcggcgttaa
     1441 tcgggtgatt ttagacagcc gcccggttca tgcatcacgt ccacacagtg aagctattcg
     1501 cgacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct gtactgacgg cgacaaatcc
     1561 gctgatccgt tttatcggta gtgatgatat gacgcaaaac cgggaattat ttcaggtctg
     1621 gttacaaaaa ttagcgcagt ggcatcagac cactacgcct tatctttttt tacatacgcc
     1681 agatattgcc caggccccgg aactggtaca taccctgtgg gaagacttac gtaaaacgct
     1741 tccagagatc ggagcagttc cggctattcc acagcaatct tctcttttct gaatttgcca
     1801 cctatcatag acaggtgcca tcggccattt taaagggagt ttgtatggta agcgcgctat
     1861 atgccgtttt aagtgcgttg ttattaatga agttctcttt tgatgtcgtt cgcctgcgaa
     1921 tgcagtaccg cgttgcctat ggtgacggtg gttttagcga actgcaaagc gctattcgca
     1981 ttcatggtaa cgcggtggaa tatattccca tcgcgattgt gctgatgctg tttatggaaa
     2041 tgaatggcgc agaaacctgg atggtgcata tttgcggcat cgttttgctt gctggtcgtc
     2101 tgatgcatta ttacggtttt catcaccgtc tgtttcgctg gcgacgttcc ggcatgagcg
     2161 ccacctggtg tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg ccctgggagt
     2221 tggttttctc cctgcgttag cgcacaatac gccactttct ttttcccgga tttttacgtt
     2281 atgtctcacc gcgacacgct attttctgcc cctatcgcca gactgggcga ctggaccttt
     2341 gatgaacggg tagctgaagt cttcccggat atgatccagc gttccgttcc cggctattcc
     2401 aatattattt ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg tacgcaggtt
     2461 tacgatctgg gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa cattcatcat
     2521 gataattgca aaattattgc catcgacaac tccccggcga tgattgaacg ctgccgtcgt
     2581 catattaacg cctataaagc ccctacgcca gtagacgtca ttgaaggtga tattcgcgat
     2641 atcgccattg aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt cctggaacct
     2701 tccgagcgcc aggcattact ggataaaatt tatcaaggac tgaacccggg cggcgcgctg
     2761 gtgctttcgg aaaaattcag tttcgaagat gccaaagttg gtgaactgtt gttcaacatg
     2821 caccacgact ttaaacgtgc caacggttac agcgaactgg agatcagcca gaagcgcagc
     2881 atgctggaaa acgtgatgct gaccgattcc gtggaaaccc ataaagcacg cctgcataaa
     2941 gccggttttg agcatagcga gctgtggttc cagtgcttta actttggttc actggtggca
     3001 ttaaaggcag aggacgctgc atgatcgact ttggtaactt ttattctctg attgccaaaa
     3061 atcatctttc acactggctc gaaacgctgc ccgcgcagat tgctaactgg cagcgcgagc
     3121 agcagcacgg gctgtttaag cagtggtcca acacggtgga atttctgcct gaaattaaac
     3181 cgtatcgtct ggatttattg catagcgtaa ccgccgaaag cgaagagcca ctgagcaccg
     3241 ggcaaattaa acgcattgaa acgcttatgc ggaacctgat gccatggcgc aaagggccgt
     3301 tctcactgta tggcgtcaac atcgataccg aatggcgttc cgactggaaa tgggatcgtg
     3361 ttctgcccca tctttctgat ttaaccggac gcaccattct ggatgtcggc tgcggcagcg
     3421 gttatcacat gtggcgcatg attggcgcag gcgcgcacct cgcggtgggg atcgacccta
     3481 cgcaactgtt tctgtgccag tttgaagcgg tgcgtaaact actgggtaac gatcagcgcg
     3541 cacatttgtt accgttaggt attgaacagc ttccggcact gaaagccttt gataccgtct
     3601 tttcgatggg cgtgctttac catcgccgtt cgccgctgga gcatctctgg cagttgaaag
     3661 atcaactggt gaatgagggt gaactggtgc tggaaacgct ggttattgat ggcgatgaga
     3721 acacggtgct ggtaccaggc gatcgttacg cgcaaatgcg taatgtctat ttcatccctt
     3781 ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt tgtagatatc cgcattgtgg
     3841 atgtgtgcgt taccactact gaagagcagc gacgcactga atggatggtt accgaatctc
     3901 tgtccgattt cctcgaccca catgatccga gtaagacagt tgaaggttat cctgcgccta
     3961 aacgcgcggt gctgattgcg cgcaagccgt aaaggtctgg tattactgcc ggatgcggcg
     4021 tgaacgcctt atccggccta caaacccttg ctaattcaat ctattgcagg gactatgtag
     4081 gcctgataag catagcgcat caggcagttt tacgtttgca taacctcagc gcccgtttcc
     4141 gggcgctatt cacttcttac gcctgaacag caggcttatc aaatgccgtt aacttcggtg
     4201 cgttacccgt atatttttcg atatacacca gcgcagagtt accggcacag gcatttgcca
     4261 gctgcgagct ggggatatcc gccgttaaca cgttcgcact gccgttttta cacaagccgt
     4321 tttccagatc tggccatgca ccttcatgca cgcataccac gccttttttg atcccgtcag
     4381 tcaccaccgc gcctgtcaaa atctgaccac gtttgttcca gacacgcacc agatcgccat
     4441 tcgcaatacc aaatcgagca gcatcttcgg tgtgaatagt gatcggttca cgatcggcaa
     4501 ccgcatattt tttacgcagt tccgcatagt taagctgact gtgtaaacgg tgtgccggat
     4561 gtgcggtcag aagctgcaac tgcttctcgt cggcggtacc cttccactca tcaggcgcaa
     4621 gccaggttgg gtgtgccggg caatccttat agccaaattt ttccagcgtt ttggagtaaa
     4681 tctcaatttt gccgcttggc gtacccagcg catttttcac cggatcggcg cggaaatcac
     4741 cataacgaac gtactgttcg ttcttctcgc tgcggcgcat ttcgatcagt ttattttgct
     4801 gccagaaggc attaaacatt ggcatagtga cgcgttgcgc acgggcccct ttctgagcgg
     4861 catcatagaa aaatttcagc cacgccattt catctttacc ttcggtatag atctcttttc
     4921 cgccaggttt gagtaattcc gcaagatcgg caaacacgtc aaaatcgtta cgcgcttcaa
     4981 attgcggagc aacagcctgc ttcatcggca caatatgctg gttgctgtaa tcaccggtca
     5041 tcgtcaagtc attgcgctca aacgatgtgg tgatcggtaa tacgatatct gcgtgtttgg
     5101 ctgctgcggt ccagtagcat tcagaaacga cgatcatctc cggtttctgc caggctttga
     5161 tcagacgatt agtatcctgg tgatgggtaa agttaccgcc acccgcccac cagatcattt
     5221 tgatattcgg atacgtttgt tctttaccgt tatgctgata tttgccgcca ggattttcca
     5281 gcgcatcgac gatacgggca acaggaatcg ccgccattcc gccatcatca gcggcctcgc
     5341 tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac gccgccaaca cgcatcgggt
     5401 tgccaccatt ggagtaatga taagagaaac caaaaccgcc gcccggtgta ccaatctgcc
     5461 ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat tgctggcgct
     5521 gaattcccca gcctgccatc agaattgtgc gattcgccgc catcagttca gcgagtttga
     5581 ctatctgcgc ttccggtacg ccagtaattt ccgccgccca gaccgcgctc ttcggcgtat
     5641 tatcgctctt acctgtcaga tactcttcaa actgcggata cccggtagtg tatttttcaa
     5701 ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct aacatcagtg
     5761 ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt atcgccaaag aattcgatgg
     5821 tttcggagcg gataggatca atggcaatca ctggtttgcc agattttttc agctgatgga
     5881 agtattccag cccttgctca tcggtactgc tccaggcaat ttttaaggta ttcagcgggt
     5941 tcattcccca cagcaccaca acctggctgt tttccagaat cagcggccag gaggcctgct
     6001 gttcatacac ctctacagaa ccgaccacat gcggcatgat cacctgtgct gccccggttg
     6061 aataatcgcc gctatgcccg gaataaccgc ccgccaggtt catataacgt tgcagtaagg
     6121 tttgcgcttt atgcaacacg ccagaagagc gccagccgta agatccggca aaaatggccg
     6181 atggtccgtt agctttacga atacgctcat gttgctcatg aatcagtttt aatgcctgtt
     6241 cccagctcac ctgtacatag gtatcttcgc cacgaccttt cgccggttgc agtggattgt
     6301 cgagatagct ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt acctgatccg
     6361 ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc gaagaaacaa
     6421 tcttgccgtc cttcacttct acgctcatcg ctccccaacg tcccgcggtg aggattttac
     6481 cgcccttctc ttctgcccat gcggggagcg gtgctgccga tgtcaccacc agcgctccag
     6541 cggcaatacc gctgtgttta ataaattcac gtcttgttaa tgccataact tcctccctga
     6601 tcaacgagga tcactgtttc tcggtaatat ctttggcgtt gtactggaaa taccgcgtta
     6661 aaatgtccag ttcgttttcg ctcatgctgg ttcgtgcccc cattcctttg gcaatggacg
     6721 gccacgcatt gacggtgtaa tggtcggcgg caatagaggc atgacaacca gcgcaatagg
     6781 tatcgtcaag tttttcagcg tattgccata gcggtttacg gtccgctaat gcgggatcgg
     6841 taagcgcacc ctgtaaagac gcctgacgcc attgattgcc gtattcgtca gcctgccatt
     6901 ccccgtttac cgtgagcgcc ttgatacctt cttcacttaa tgtggctagc gccagccgtt
     6961 gacctgccgc caggtagagc gtgttttcac tgccctgcat ttgataaccc tgcaacagaa
     7021 cgatcggctg tttgccactg gcatcaacga cggtgagatc ggtaccagga ttcacggtag
     7081 ccagctcgcc tatgcgagaa gttttgaaag gataaatatg tgcgccatta gtcactgaag
     7141 tagcggcctg actttccagc tcatgcgccg cgttgtcatc catttttatt tctggcggaa
     7201 aatgggcaat gcctttatga caatcgatac aggtttcgcc gcccttttgt gctttgttat
     7261 gcattttctg cgcagattca ctttgcgagg caatatccat ggcatcaaaa gaatggcaac
     7321 tacggcacgt tgcagagtca gttgctttta attctttcca tactgtttcg gccatttcct
     7381 ggcgatgagt ttcgaactta tcgtcactgt ctattttgcc gctaacaaat tcatgataaa
     7441 tatctttaga tgcttttaat ttagcaaata aataatccat ccctgacttt ggaatatggc
     7501 aatcggcaca ttccgcacgt atccctttct ggttcgaaaa gtggacagtt ccctgatatt
     7561 cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc gacgttttat
     7621 gtaaggcttt ttgcgccagc aataacccgc cgccaccaac cacaaccgct atcagcaaaa
     7681 acaataaccc aatgcgtttt ttccctcgca tagattcctc taaataaatt ccagaacacc
     7741 gaaatcattg tataattaaa tacacaacga attaaccgct tgcataactc ggcacaacac
     7801 tgcctgaaat aatcgataaa gaatatgatt tattacaatg taatcattaa ttgctaagga
     7861 ataacccagt tgctatttag aatatatatt tatcagtttt agcaatttaa atcccataat
     7921 caatgtgaat ataaaaaata tttttagcac aacgctatgc gcactgtcac ccgctcaaca
     7981 gatcacggtt atcgttcgtt ttttatactg ctcagggata aaaaaaggcc cctgttgaaa
     8041 ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac ggtaaaaatc
     8101 atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc ccgtctacga
     8161 tataacgcga atactcgtcc gcgtgttcat ctgatgacat ggacaatcct tgattacgat
     8221 aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc actccggcat
     8281 cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc agccataatg attggagcat
     8341 cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct gatttttgcc
     8401 ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta tataaagggt
     8461 tagcgcacat atcgaaggcg cgatgaaaag tcactgccag cggaccggca gcagccatta
     8521 ttttttccat tcgtggcata tcgacattcc catcaacatc gagaacgccc gtcaccagtc
     8581 caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca aactcaccgt
     8641 cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg atcgtcaccc
     8701 gctggcgcac ggatttcagt ccccccaacg acggcgttaa gcccccctct tttggggctg
     8761 cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagtgcacat tccatgctgt
     8821 aacagcaaat ttccagtaat gccatttttg ctccttaatt acgctgactg ctcgctggca
     8881 acgatctctt caatggacca cggatgaaac ttaatagttg ttttgccatc ggtgaccgcc
     8941 agcgtcggat tcggtaatcg ctcatgctca ccttttggtg aactggtttt aactgagatc
     9001 cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt cagcgtttct
     9061 ggatcacccg gcagaacgat ttcaatatgt tcccaacctt cgtgtgggta acgtttttcc
     9121 ccaggccacg gtagctccac attggaaaac tgccagtgcg caacctgtac cggttcatgc
     9181 aatttaaaca gacaaatcgg tctgccattg atcatttttt ctgacaaaag ctcgccacac
     9241 tgttcaaaac cgcgacgcca gcgctcagcg gtgacgtttt gatggcaacg caaagaaatg
     9301 tgatcggcag tcagcggagt gatatccaac cccagacggc gggaaagttc atctaatgcg
     9361 tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga ttgccagttc
     9421 gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc cctgtgccaa
     9481 ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata cgccacgcgc
     9541 acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct ttaactggtg
     9601 cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc aggctcttct
     9661 ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt gcgaaccgca
     9721 gattcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca tgatggcagt
     9781 tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga ctcatctgga
     9841 tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca acattttcct
     9901 tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc gtctcggtgt
     9961 tgctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg tggcgaaaga
    10021 gatgcacgtc ggtcacctgc gctctaccat tattggtgac gcagcagtgc gtactctgga
    10081 gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca ctcagttcgg
    10141 tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa tggagctggc
    10201 tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg aagagtttgc
    10261 cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt tccgcgagat
    10321 gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccaa atcacttacg atcgtctcaa
    10381 cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc tacaacccaa tgctgccagg
    10441 aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg cgaccgtcgt
    10501 attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca ttcagaagaa
    10561 agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc gttatgaaac
    10621 actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac acctgatgca
    10681 ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc tggaacacca
    10741 catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg cgggtggtac
    10801 agtgaaactg gcagacctgc tggatgaagc cctggagcgt gcacgtcgtc tggtggcaga
    10861 aaagaacccg gatatgccag ctgacgagct ggaaaaactg gctaacgcgg ttggtattgg
    10921 tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct tcgactggga
    10981 caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgctt acacgcgtgt
    11041 attgtccgtg ttccgtaaag cagaaattaa cgaagagcaa ctggctgcag ctccggtaat
    11101 catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg aagaaaccct
    11161 caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt gcttacctgt acgatctggc
    11221 cggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa acgaagaagt
    11281 gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctgaagc tgggtctgga
    11341 tacgctgggt attgagactg tagagcgtat gtaatcgatt tttcgtgaga gtgaagcctg
    11401 atcagggtta gccgatcagg cttttttatt gccatctaaa tgtattctga aaacggacat
    11461 gccattgttt tctcactgtt gagtaagagg ccagaagcgt aatatccagg cccaggaaaa
    11521 cgataactgt tcaatttaag gaataccgaa gtgtttaaat ttcttgtatt aacattaggc
    11581 attatctctt gccaggctta cgcagaagat acggttatag taaacgacca tgacacttca
    11641 gccatcaaag attgttggca aaaaaattca gatgatgata ctgacattaa cgtgatcaaa
    11701 tcatgcctgc gacaagaata caatctcgtc gatacgcaat taaataaagc ctatggtgaa
    11761 gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacttga tactgaacaa
    11821 cttaacttgc ttaaaaa
//
LOCUS       DAJTXY010000118        11574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTXY010000118 DAJTXY010000000
VERSION     DAJTXY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.118"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..336
                     /locus_tag="OO566_003320"
     CDS             1..336
                     /locus_tag="OO566_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6606493.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            429..512
                     /locus_tag="OO566_003321"
     CDS             429..512
                     /locus_tag="OO566_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606494.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            612..1433
                     /locus_tag="OO566_003322"
     CDS             612..1433
                     /locus_tag="OO566_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6606495.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1472..1801)
                     /gene="mdtI"
                     /locus_tag="OO566_003323"
     CDS             complement(1472..1801)
                     /gene="mdtI"
                     /locus_tag="OO566_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6606496.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1788..2153)
                     /gene="mdtJ"
                     /locus_tag="OO566_003324"
     CDS             complement(1788..2153)
                     /gene="mdtJ"
                     /locus_tag="OO566_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6606497.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2564..3598
                     /gene="tqsA"
                     /locus_tag="OO566_003325"
     CDS             2564..3598
                     /gene="tqsA"
                     /locus_tag="OO566_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6606498.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQLLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3623..5011)
                     /gene="pntB"
                     /locus_tag="OO566_003326"
     CDS             complement(3623..5011)
                     /gene="pntB"
                     /locus_tag="OO566_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6606499.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5022..6554)
                     /gene="pntA"
                     /locus_tag="OO566_003327"
     CDS             complement(5022..6554)
                     /gene="pntA"
                     /locus_tag="OO566_003327"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6606500.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7078..8022
                     /locus_tag="OO566_003328"
     CDS             7078..8022
                     /locus_tag="OO566_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6606501.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8208..9590
                     /locus_tag="OO566_003329"
     CDS             8208..9590
                     /locus_tag="OO566_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6606502.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9627..10349
                     /gene="folM"
                     /locus_tag="OO566_003330"
     CDS             9627..10349
                     /gene="folM"
                     /locus_tag="OO566_003330"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6606503.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10346..10681)
                     /locus_tag="OO566_003331"
     CDS             complement(10346..10681)
                     /locus_tag="OO566_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6606504.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPIFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            10810..11553
                     /gene="rstA"
                     /locus_tag="OO566_003332"
     CDS             10810..11553
                     /gene="rstA"
                     /locus_tag="OO566_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6606505.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2929 a         2969 c         2796 g         2880 t
ORIGIN      
        1 atgaaaaaac aaattgaggg tatgacaatg aaaaaagtat tagctctggt tgttgccgct
       61 gctatgggtc tgtcttctgc cgcctttgct gcagagactg cgaccacacc tgctccgact
      121 gcgacgacca ccaaagcagc gccagcgaaa actacacatc ataaaaaaca gcataaagca
      181 gcacctgccc agaaagcgca ggcggctaaa aagcatcata aaaatacgaa agctgaacag
      241 aaagcccctg aacaaaaagc gcaggcagcg aagaaacacg ccgggaaaca cggccatcag
      301 caaccggcaa aacctgctgc acaacccgca gcgtaagttt tcaacagtaa tgctggcgcg
      361 ccccctcgcg cctgaaaatt acggtgctaa gcgggtaacg tttagcaccg cctttagcgg
      421 agggtagtat ggtgggccgt tatcgctttg agttcattct tatcatcctt attttatgcg
      481 cactgattgc cgcccgtttt tatctttcct gactgtagtt atctgatttt actcccactt
      541 tcatcccgtc ccgtctatag tatttatgag ggtttgcttt taataatcat aattacccac
      601 cagagtgtga tatgcgtaca accattgctg tagtgttggg tgcaattagt ttgacgtctg
      661 cttttgtgtt tgcagataaa ccagacgttg ccaaatcggc aaacgatgag gtcagcaccc
      721 tgttttttgg tcatgatgat cgtgtgccag tgaatgacac gacccaatca ccgtgggatg
      781 cggttgggca actggaaacg gccagcggca atttatgtac ggcgacgctg attgcaccca
      841 atctggcatt aacggcagga cactgtttat tgacgccgcc taagggtaaa gcggataagg
      901 cggtagcgct gcgttttgtg tcaaataaag gtctttggcg ctatgagatc cacgacatag
      961 aaggccgcgt tgatccgaca ctgggaaagc ggttaaaagc agatggggat ggttggattg
     1021 ttcctcccgc agctgcaccg tgggacttcg gattgattgt gctacgtaat cccccttctg
     1081 gcattacacc gttgccgtta tttgagggag ataaagccgc gcttactgcc gcattaaaat
     1141 cggctggtcg taaagtgact caggcgggat accctgaaga tcatctcgat acgttataca
     1201 gtcatcaaaa ctgtgaagtg actggctggg cgcaaacgtc ggtgatgtca catcagtgcg
     1261 ataccttgcc gggtgacagc ggttcgcctc tgatgttgca taccgatgac ggctggcaat
     1321 taattggggt gcaaagttcg gctcctgccg cgaaagatcg ctggcgcgcc gataaccggg
     1381 ccatttctgt taccggtttt cgcgacaagc tggatcaact gtcgcaaaaa taatgttcaa
     1441 acgctgcccg acagcgcggg cagcgtcttc atcaggcaag tttcaccatg atcattccag
     1501 ccaacagcaa gaccaggcca atccagcctt tacgatttaa ccgttgacca aacaagatcc
     1561 aacctgcggc taacgtggcg gcaataccaa acccgcccca caatgcataa gcgacagaca
     1621 agtcgatccc tttaacggcc tgagaaagcg cactaaaggc cgccagcacc gccgccaggg
     1681 agagcaagcc aaatattttg cgacgaaagc cgtcagaaaa tttcaaaaag acgttagcaa
     1741 cgatttccag cacgattgcc aatgccagcc aggcggcgtg aacccattca aactgcgcca
     1801 tggttcacct ccagttcagg tttacgcgct ttacgggtac ctgatttgat caacactatc
     1861 ccggcgacca gggtggttaa cccggcaatt ttcatcagcg ataaactttc gtcgaataac
     1921 aaaacgctaa acaaggtaat aaataaaata ccgatacctt cccacagcgc ataagctacg
     1981 cctaaggcga tttttttaac ggcgaaagag agaaatatat acgacagaga aatcatcacc
     2041 agcattaaaa taaagccgcc atttccctca ctgacgctcg cccatttcat tgacagcgta
     2101 ccggtaattt ctgtagcaat agccagacct aataaaatcc aataaatata cattgtcctt
     2161 ctcctgcaag agaactattt taattttcgc ttaattcagc gaaaccaaag taaagtggtc
     2221 aagccctgcg catagggcaa gcttaggcag aagaaaggac taaagcgcgt tgcgccagtg
     2281 ctgctcacct acgagcaaga tagtggatga ggtacgaaga tgggtaaatg tagaaaataa
     2341 cgtcctgaac aaattgtcca taatattaca attatccgca gtgttgcttc tcgtcatcgc
     2401 agatgataat tgtcctcggt agttgaacac gcctgatttg tatcatagct taagaattaa
     2461 ctcaaaatat tttcacttct ttacctgagc ggtttgattt tcgttatgat gacggagcga
     2521 aaaagacatt attattagca aaggaagaaa aacggggaca agcatggcaa agccgatcat
     2581 cacgctcaat ggcctaaaaa tcgtcattat gttgggaatg ctggtcatta ttctctgcgg
     2641 tatccgtttt gccgccgaga tcatcgtgcc gtttattctc gcattattta ttgctgttat
     2701 tcttaacccg ctggtgcaac atatggtccg ctggcgtgtg ccgcgtgtac tggcagtgtc
     2761 gattttgatg acaatcatcg tgatggcgat ggtgttgcta ttagcttatc tgggttccgc
     2821 gctcaacgag ttgacgcgga cgttaccgca atatcgcaac tctattatga cgccgctgca
     2881 agctcttgag ccgttgttgc aacgcgtagg gattgacgtc tcagttgacc aactggcgca
     2941 ttacattgat ccgaacgcgg cgatgacgtt gctcaccaac ttattgacgc agttatctaa
     3001 tgccatgtca tcaatatttt tattgctgct gacggtgctg tttatgctgc tcgaagtgcc
     3061 acaattgccc ggaaaatttc agcaaatgat ggcgcgtccg gttgaaggga tggcggcgat
     3121 tcaacgtgcg attgacagtg tttctcatta tctggtgctg aaaacagcca tcagcatcat
     3181 caccggcttg gtcgcctggg cgatgctcgc cgcactcgat gttcacttcg cttttgtctg
     3241 gggattgctg gcctttgcgc ttaattacat ccctaatatt ggttcagttc tcgcggcaat
     3301 cccccctatc gctcagctac tggtgtttaa tggcttctac gaagcgttgc tggtgctggc
     3361 gggatatctg ctgattaatc tggtcttcgg caatattctg gagccgcgca tcatgggacg
     3421 tgggctgggg ctttcgacat tggtggtatt tttgtcgttg attttttggg gatggttgtt
     3481 aggaccggtg ggtatgctgc tttccgtgcc gttgacaatt attgtcaaaa ttgcgcttga
     3541 acaaacagcg ggaggtcaaa gcatcgccgt tctgttaagc gatctcaata aagagtgacg
     3601 gcctccgcag aggccgtcag ggttacagag ctttcaggat tgcatccacg ctggctttgg
     3661 cgtcaccaaa cagcatgtgg gtgttttcct tgaagaacag cgggttttgc acaccagcat
     3721 agccagtgtt catcgaacgt ttaaagacaa tcacgttctg cgctttccac acttccagca
     3781 caggcatacc agcaatcgga ctcttcggat catcctgcgc cgccgggtta accgtatcgt
     3841 tagcaccaat caccagtacg gtatcggtat cagcaaagtc atcgttgatc tcgtccattt
     3901 ccagcacgat gtcatacggt acttttgctt cagccagcaa tacgttcata tgtccaggca
     3961 aacgccccgc aaccgggtgg ataccgaaac gcacattaat actacgagcg cggagtttct
     4021 cggtaatttc agcgacagga tattgcgcct gcgcgactgc catgccgtac cccggagtaa
     4081 tgatcactga atgggagttt ttcagcagtt ccgctgtctc ttctgcggtg atttcgcggt
     4141 gctcacccac ttcctgatca tcgccagtag aagagccgtc ggtgccgaaa ccacccgcaa
     4201 taacgctgat aaaggaacgg ttcatcgcct tacacataat gtaagaaagg atagcccccg
     4261 aagaaccgac cagcgcaccg gtcacaatca gcaggtcgtt gctgagcata aagcccgcag
     4321 ccgcagccgc ccagccggag tacgagttca gcatcgacac caccaccggc atatctgcac
     4381 caccgatgga ggcgactaaa tgccagccga ataccagcgc aatggcggtc attatcagca
     4441 atgccagcac ttgcaggccg acgctgtccg tgcgaacaaa tacaatcagc agcaagaagg
     4501 aaacgaccag agccgccagg ttcattttgt gacggtttgg cagcatcaac ggtttagacg
     4561 aaatcttgcc acacagtttg ccgaacgcca ccaccgagcc cgtgaacgtt accgccccga
     4621 tgaagatacc gaggaacact tccgtcaggt gaatattgac cagaatcggt gccattcccg
     4681 cgtcatgatg cagataactg ttaaagccaa ccagcactgc cgccagaccc acgaagctgt
     4741 gcaggatcgc caccagttct ggcatttcgg tcatttcaac tttcttcgcc agccggatac
     4801 caattgcccc accaatgacc atcgccagca agatccagcc aacattaccc gtatccggtc
     4861 caaaaatggt tgcgattaac gcaatcgcca tcccggcgat accgaagttg ttaccctggc
     4921 gagacgtttc atgtttcgaa agaccggcca gactgaagat aaacaggatc gcggcaacaa
     4981 tgtatgcagc tgtaactaat cctccagaca tatgttaccc cttaattttt gcggaacatt
     5041 ttcagcatgc gctgagtcac ggtgaagcca ccgaaaatat taatgctggc tataagcacc
     5101 gcgataaaac taaggaagct aacccagcct ccctggccaa tctgcaacag tgctccgaca
     5161 acaataatcc ctgaaatcgc gttggtaacc gacatcaacg gtgtatgcag cgcgtgcgat
     5221 acattccaca ccacgtaata accgacaacg caggccagcg cgaaaacggt gaagtgccca
     5281 agaaactctt tcggcgcaac gcttgacagc caaccgaaca ggatgattgc cagcgccatc
     5341 aacgcgtatt tacgccacgg tgagcaggca catttttcct cagttttcac ttccggtgcc
     5401 gctttttgtg ccgcctgcgg ctgagctgat acctgaatcg gcggtgccgg ccaggtaatt
     5461 tcgcccgcac ggatcacggt cacgccgcga atcaccacat catcaaaatc aacagtgata
     5521 ttgccgtctt tctctttgca caacagtttc agcagattaa cgaggttagt accgtaaagc
     5581 tgtgaggatt gcgtcggcag acggcccgga agatcggtat aaccaatcac tttgacacca
     5641 ttttccgtac tgaagatttc acccggcacg atgtattcac agttgccacc gttttgggct
     5701 gccaggtcga caatcacact gcccgccttc atggagtcaa ccatttcacg ggtaattagc
     5761 ttcggcgctg gtttgcctgg aataagcgcg gtggtgacaa tgatatcgac ctcttttgcc
     5821 tgggcggcaa agagttccat ttccgctttg atgaacgcgt ccgacatcac tttggcatag
     5881 ccatcgccgc tgcccgcttc ctcgttaaaa tccagctcga ggaattccgc gcccatactt
     5941 tgaacttgtt ctttcacttc cgggcgggtg tcgaatgcac gcacaatcgc gccgagactg
     6001 tttgctgcgc caatggcggc caaacctgca acaccggcac caatcaccat cacttttgcc
     6061 ggtggcactt tcccggccgc agtgatttgc ccggtaaaga agcgcccaaa ttcatgtgcc
     6121 gcttcaacaa tggcgcgata accggcgata ttcgccatcg agcttagtgc gtccagcgat
     6181 tgtgcgcgtg agattcgcgg cacagagtcc atcgccatca cggtcacgtt acgttccgca
     6241 agtttttgca ttaattccgg attctgcgca ggccagataa aactcaccag cgttgtcccg
     6301 ggattcagta acgcaatttc atcatctaac ggcgcattga ccttcagaat gatctctgac
     6361 tgccagacgc tattcccttc tacaatttca gcgcccgctt gcacaaacgc tttatcgtca
     6421 aaacttgcca gttgacccgc gccgctctct accgcgacgg taaaacccag tttcagcagc
     6481 tgttccactg tttttggcgt tgctgcaaca cgggtttcat tggttaaccg ttctcttggt
     6541 atgccaattc gcatgatatt cccttccatc ggttttattg atgatggttt gcctgtgtca
     6601 ggagccacac aagccgctca tgtacgagct aaatgttact ccgttaaaat aaattagtaa
     6661 caaacgccct ataacgtact gaaaattatg cctgtgatct agcgccaaaa atcagtattt
     6721 cggcgtgaac tcgcaaaata ttaacgattc agccgtgata gtgggataaa caccttagaa
     6781 cgccggataa agactgataa ttgtcttcga cggtcgggta aaacgagaca tcgccccggc
     6841 acgaatcact acttaacatt aaattaactt atacaattca gttgcttcag tagtaatgat
     6901 gctgatacgg ctgtttttta agcatagacg gtcatttgag caggattaaa attggtttaa
     6961 ggaatgtgat atgaaaaatg acgcagacag ttacaccgtt taaatgcaat aatcagccac
     7021 gtttctcgtt aataacaata ccagtacctg gtttgcgcaa ggcgaaggat tatttttatg
     7081 aagcttaaga acaccctcct ggcgtcggca ctgctttctg ctacggcatt ctccgttaac
     7141 gcagcaacag aactgacacc ggagcaagcg gcagcggtta aaccttttga ccgtgtagtg
     7201 gttaccggtc gttttaatgc tattggcgaa gcggtgaaag ccgtttctcg tcgcgcagat
     7261 aaagaaggtg ccgcctcttt ttatgttgtc gacacttctg attttggtaa cagcggtaac
     7321 tggcgtgtgg tcgctgacct ctataaagcc gatgctgaaa aagcagaaga aacaagtaat
     7381 cgcgtaatta acggtgttgt cgaactgccg aaagatcagg ctgttctgat tgaaccgttt
     7441 gacacggtca ccgtccaggg cttctatcgt agccagccag aagtcaatga tgccatcacc
     7501 aaagcggcaa aagcgaaagg tgcctactct ttctacatcg ttcgtcaaat cgatgccaac
     7561 cagggcggca accagcgtat tactgcattc atctataaaa aagatgctaa gaaacgtatc
     7621 gtccagagcc cggatgtgat cccggcagat tccgaagcag gacgtgcagc tctggctgcc
     7681 ggtggcgaag ccgcgaagaa agttgagatc ccgggtgttg cgactaccgc atcaccaagt
     7741 tctgaagtcg gtcgcttctt tgaaacccag tcatcaaaag gcgggcgtta caccgtcacg
     7801 ctcccggatg gcactaaagt cgaagaactg aacaaagcga cagcagcgat gatggtcccg
     7861 ttcgacagca tcaaattctc tggcaactac ggcaacatga ccgaagtctc ctatcaggtt
     7921 gcgaaacgtg ccgcgaagaa aggtgctaag tattaccaca tcacccgcca gtggcagaaa
     7981 cgtggtaata acctgaccgt cagcgcagat ctgtataaat aactattcag ttactcctat
     8041 aaggcggctt gatgccgcct ttcgcatttt tagtcacaaa tttccccgcg accattgcat
     8101 gcagacacat cttttcgtaa aatcccgcgc ttcagtggca aactccattt ttatgcgttt
     8161 tgccctaata tttattcttc ttatcacgtt ttaatcactg gatatcgatg gaaaagaaac
     8221 tgggactgag cgcactcacc gcgctggtat taagctcaat gctgggcgcg ggtgttttca
     8281 gtctgccgca aaatatggcg gcagttgcca gcccggcagc actgctcatc ggctggggta
     8341 ttactggcgc tggcatttta ttgctggcct ttgccatgct gatcctcacg cgcattcgtc
     8401 ccgaacttga tggcggtatc tttacctatg cccgcgaagg gtttggcgag ctaatcggct
     8461 tttgttccgc atggggatac tggctgtgcg cggtcatcgc caacgtctcc tatctggtga
     8521 tcgttttttc cgcgttaagc ttttttacgg acacgccgga attgcgcctg tttggcgatg
     8581 gtaatacctg gcaatcgata gtcggtgcat cggctttatt gtggattgtt cactttttga
     8641 tcctgcgcgg ggtgcaaacc gctgccagca ttaacctggt ggcgacactg gcaaaattgt
     8701 tgccgctggg tctgtttgtt gtgctggcga tgatgatgtt caaactggat accttcaagc
     8761 tcgacttcac cggacttgca cttggcgtac ctgtttggga acaggtgaaa aacaccatgc
     8821 tgatcaccct gtgggttttc attggtgtgg aaggtgccgt ggttgtttct gcgcgtgcgc
     8881 gtaataaacg tgatgttggc aaagcgacac tgctggcggt tctctccgct ctgggcgttt
     8941 acttgctggt aacgctgctt tcgctgggtg tggtggcacg tcctgaactg gctgaaattc
     9001 gtaacccgtc aatggccggt ctgatggtgg aaatgatggg accatggggc gaaatcatca
     9061 tcgctgccgg tttgatcgtt tccgtttgcg gcgcgtactt aagctggacc atcatggcgg
     9121 cagaagttcc gttcctggct gccactcata aagcattccc acgcattttt gcgcgccagg
     9181 acgcacaagc tgcgccatcc gcctcactgt ggttgaccaa catctgcgtg caaatttgtc
     9241 tggtactcat ctggctgacc ggttccgatt acaacacgtt gctgaccatc gcctcagaaa
     9301 tgattctggt gccctatttc ctggtcggcg cgttcttgct gaaaatcgca acccgtccac
     9361 tgcataaagc ggtaggtgtc ggtgcctgca tttatggctt atggttattg tacgcctctg
     9421 gtccaatgca cttgctgtta tccgttgttc tgtatgcacc ggggctgctg gttttcctgt
     9481 atgcgcgcaa aacgcataca catgataatg tactgaaccg tcaggaaatg gtgctgattg
     9541 gcatgctgtt aattgcctca gttccggcga cctggatgct ggtgggataa cgttcatccc
     9601 atcgttggat agcaaaggag ataacgatgg gtaaaaccca gcccttgcca atattaatta
     9661 ctggcggagg tcgtcgcatc ggcctcgccc tcgcatggca tttcattaat caaaagcaac
     9721 cggtgattgt cagctatcgg acacactatc cagccattga tggactgatt aatgcaggtg
     9781 cgcagtgtat tcaggctgat ttttcgacca acgacggtgt gatggcgttt gccgatgaag
     9841 tactaaaaac cacccatggt ctgcgtgcta ttttgcataa cgccagtgcg tggattgcgg
     9901 aaaaaccggg tgcgccactg gccgacgtac tggcttgcat gatgcagatc cacgttaata
     9961 ccccatacct gctcaaccat gcgctggaaa gattactgcg tgggcacgga cacgccgcca
    10021 gcgatatcat tcactttacc gattatgtgg tggagcgcgg cagcgacaaa catgttgcgt
    10081 atgctgcaag caaagcggca ctggataata tgacccgctc gtttgcccgc aagctggcac
    10141 cggaagtgaa agtgaattct attgcgccat cgctgatcct gtttaatgaa catgatgatg
    10201 ccgaatatcg acaacaggcg ctgaataaat cactgatgaa aaccgcgcct ggcgagaaag
    10261 aagtgatcga cctggtcgat tacttactta ccagttgctt tgtcaccgga cgcagtttcc
    10321 cacttgatgg cggtcgtcat ctgcgttaat gcagctttat ccagcatata atcaacaccc
    10381 atgcgcttat cccccaacac gcaacagaac caacaaaagc ggcgggcaag cgcatcattc
    10441 cggtgaaata ccacagcgac accaggtaaa caaaataggg aataatcgac cacatactaa
    10501 aaatgatggt tgcgcgtaag gcttcaatgc cgcgttcgct ggcaacaata taatgcgcga
    10561 taagcgcaaa gatcgggaaa agtggaatca gcccggcgat ataataattt ttcgtttttg
    10621 ctaaaacacc aatcaacagc actaccagcg caccgagcgc ggctttgatt accagcccca
    10681 tctttttacc ttaacacttc cataacaagt catcagtaga atacctgatg aaaacttgtt
    10741 tagaaacgat tgatagtaag taaaaacagc gcggtgtatt gtgacgtttt tatatctacc
    10801 gtgaatgtta tgaacactat cgtatttgtg gaagatgatg cggaagtcgg ttcactgatt
    10861 gccgcgtacc tggcaaaaca tgatatgcag gttaccgtag aaccgcgcgg cgaccaggcc
    10921 gaagaaacca ttttgcgaga aaatccggat ttggtgttac tcgacatcat gctaccaggc
    10981 aaggacggca tgaccatttg tcgtgattta cgcgcaaagt ggtctggacc gattgttctt
    11041 ctaacctctc tcgatagcga tatgaaccac atcctggcac tggaaatggg tgcctgcgac
    11101 tatattctca aaacgacgcc ccctgctgtt ttgctagcgc gtttacgttt gcatttgcgt
    11161 cagaatgagc aagccacact gaccaaaggt cttcaggaaa cgtctctgac tccctacaaa
    11221 gccctgcatt tcggcacgtt aactatcgac cccatcaacc gcgtagtcac cctggctaac
    11281 actgaaatct cgctctcgac agctgatttc gaattattgt gggaactagc tacgcatgcc
    11341 gggcaaatca tggaccgcga tgcattgctg aaaaatttac gcggcgtcag ttatgacgga
    11401 ctggatcgta gcgtggacgt ggctatttcg cggttaagaa aaaaactgct cgataacgcc
    11461 gcagaacctt atcgcattaa aactgtgcgt aacaaaggct atctttttgc gcctcatgca
    11521 tgggatagtg acaataaaca atcagatgac taatatttgt tcatctgaaa aaga
//
LOCUS       DAJTXY010000119        11557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTXY010000119 DAJTXY010000000
VERSION     DAJTXY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.119"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(1..339)
                     /locus_tag="OO566_003333"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="OO566_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
     gene            complement(655..1299)
                     /locus_tag="OO566_003334"
     CDS             complement(655..1299)
                     /locus_tag="OO566_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606506.1"
                     /translation="MMKQGMANSISLTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1320..1589)
                     /locus_tag="OO566_003335"
     CDS             complement(1320..1589)
                     /locus_tag="OO566_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6606507.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPEKLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1668..2306)
                     /locus_tag="OO566_003336"
     CDS             complement(1668..2306)
                     /locus_tag="OO566_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6606508.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2291..3523)
                     /locus_tag="OO566_003337"
     CDS             complement(2291..3523)
                     /locus_tag="OO566_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6606509.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHILEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            complement(4024..6705)
                     /locus_tag="OO566_003338"
     CDS             complement(4024..6705)
                     /locus_tag="OO566_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6606510.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVKRAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSLQLS
                     YYEKNIREKQISLNEYEEVFKKSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFSAET
                     YRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(6946..7992)
                     /locus_tag="OO566_003339"
     CDS             complement(6946..7992)
                     /locus_tag="OO566_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HCR6606511.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSAGTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(8096..8245)
                     /locus_tag="OO566_003340"
     CDS             complement(8096..8245)
                     /locus_tag="OO566_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606512.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            8667..8858
                     /locus_tag="OO566_003341"
     CDS             8667..8858
                     /locus_tag="OO566_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6606513.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMRDGYPHPCM
                     QPMYNFLGKFKPEPREETE"
     gene            complement(8911..9144)
                     /locus_tag="OO566_003342"
     CDS             complement(8911..9144)
                     /locus_tag="OO566_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606514.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(9240..9863)
                     /locus_tag="OO566_003343"
     CDS             complement(9240..9863)
                     /locus_tag="OO566_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6606515.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(9952..10461)
                     /locus_tag="OO566_003344"
     CDS             complement(9952..10461)
                     /locus_tag="OO566_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606516.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            10919..11377
                     /locus_tag="OO566_003345"
     CDS             10919..11377
                     /locus_tag="OO566_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606517.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT         3234 a         2874 c         2311 g         3138 t
ORIGIN      
        1 acctgattct ggaaaaggac tttgtggatt gttgctattg ccagtgagat tgaatgaagt
       61 cgttttaact cgctttgaat acgtcttgcc cccagatttc gctgagaacg taactccaga
      121 atcagagcga cttcgcaagt gccggttttc gttgatggtg aacgcttggg gcgacggctg
      181 tggctttcca gaccagcgat gccctgagcc agatatctgc gccaccattt tacgcaatgt
      241 gggccgagaa atgccacagc gacggcatac aaatcccgca tcaccggacg tttcatagag
      301 ttttacccac tgtagccgct gctgaatttc cctgttcata atttggcatt agttcagctc
      361 aatcccgact taaaaagtcc ctacaggatg ttccggacct gaggcacagt tcatatcctt
      421 atccataaaa aatttagcta tgtctatagc attaatcttg acaagacgac gttagggcct
      481 ggcgatgaat catttcccct gccagatatc ctttggggtg caccagaact accgttttat
      541 atctatataa ctgaaacgtc cctcgccatc cttcatattt tgatgtagta acaccattca
      601 ctttcatcta ccccccgttt tattaacata tagtaaaaat cacctcgccg cattttattc
      661 ttttgaagta aaatcattta ccagttcact taaagctctc ttttgttcat catcatacag
      721 agattgaatc cggaatttaa tctcttctgc aaccagtctt gcatcgggct cataaaatat
      781 ttttgttatt cgctgtttct gatgttcagg ctggtcttcc atatttcgta aaaccagatg
      841 aaataatata tcataaagtg cgcaatgcca tgaataacca tcattggtta aaataagctc
      901 ccaaacccgc tcctgctcgg caataagctg cagaacaaag gactcaacct gttctgtttg
      961 ttcgggggca acacggtata gcggagcaat cacagcaaaa gatagttcat ttaaccagga
     1021 ttccgcttcc ttctcgctcc tgaacggtgg acagttttgt attcttcgtt cgtattcacg
     1081 ggtaatctgc tccagaatat cctccggcgg tgtcagccac ttattcctga gcaatacact
     1141 ggttgatgga tgacagctgg ctatttcagc aataatttca cgtaatgttt ccgggctgaa
     1201 cttcttcagc tgattgtagt gctcccgggt aaaaccatgt tcatcgcata aaagttgcca
     1261 tgtcagtgat atactgtttg ccattccttg cttcatcatt aatacctgtt acctgaatat
     1321 caacagaccg gtgtaccttt tctgaatacc tccccggacg gagtggcaat ccccattcgc
     1381 ctcagcgtgg catctgcagc tttcgccacc tcgctgatat ccagcttttc aggtagcggc
     1441 ggggacgata tctgcgccag acaataattc cggcgcaaca acaaccactc ccgttgtgct
     1501 tctgtacatt caacatttcc cataacaaaa agccagcggt cagaagctct gcgccagcaa
     1561 ccagcagcct ccagtttctg cgcaaccatg tcttccacaa cttccggggt actctttttc
     1621 ctgctcaaaa cattaactca ctgaaataat atacatatct gacggtctca gcgaaccgtc
     1681 catgccgggc tgaaatcctc ttcctgaaac agctccgtca gcccgctaat ctgtttcaat
     1741 ctgagaactt catcctggtc catacccagc tccgtaccaa tgcgttcatt cgtccagccc
     1801 aggcgcgaca ggtccctgac aatatcagac attgaggtta tctgatgttt gcccctggcc
     1861 cggttatgtc ggatggtcgc agcaatacgt gaggcctgtc cctttctctc cggattaata
     1921 cagaccaccg gcagccagcc cttcagacgt tttcccgtac ctgcttccct gcccagcacc
     1981 tggcgatgaa aaccatccac caccaggtaa tggttctttt cctcagacac caccaccggt
     2041 tgtgtgaaac catctttctc cagtgactgc ttcagcagtt tcttctcacc cggcgccatg
     2101 acattaggat tgtaatcatt ggccaccacc tcatctgctt ttacccacag cacacagtcc
     2161 actggctcat gtgcaaaagg actgacttca tgaagcactc tgcgcaggtg attaagggcc
     2221 tgtatggtct cttcttctgg tcgttcctgg ctgaagtact gacgaatcat gttgccaatt
     2281 atctccggtg tcacagaatc ccccattcct tcctcctttc tttcatacgc tgcagataac
     2341 gttcatagtg ccggggtttg ttcggactga aggagagggt ccggcaccag aaatcattct
     2401 ttataagtgt cttacagata cgccgccagg acggaatatc ccggctcccc aggtcattct
     2461 cctgttcatc cgggatgtca tccgggaagc cccgcgtctg ataccagcgc aggtagacag
     2521 caattttatt acggtaatgt tctgccgttc tttccggcat cacatccagc aggaacatcg
     2581 catagctgcg ccaggtatga tgggctggtt ttgatatacg tttacgcagg gcaaaatagg
     2641 caccgctttc attggcataa agcgccccgc tggcagcgcc tgacacccgc tcacacatcc
     2701 tggcccaggt ttccggctcc agaatatggt aaagccacag ccccttacgt tgttcaggac
     2761 caaaaggttc acagacacgc atgttgcgta acggcacgtc ggcacggtac atcaggtcat
     2821 acagaggatt gtaaatggca caagctctgg cgttatatat ccagatatcg cgggctttcc
     2881 agtcatacaa cggatacatg gtgtaataaa acccttcagg cgatgccgtg gtccagggct
     2941 tatcatcggc atatctcagt ttgcgctgag acaccagccc cataaagcga ttgagggatt
     3001 catccgcacg aacaccggtc agcactgcca ctccacaccg gttaccggca aaccaggaag
     3061 aaaatgccgg aacaaattct tcaaacgtca tggcataccg gaaaaaggga aaatatgtca
     3121 tatccgtaat ggcctcttcc ggtggctggc gaacccaggt cactcctggc tcccagcata
     3181 tccactccgg ctgaaactgc gagacgccgt ttaccgtggt caggggaagt gccacccagt
     3241 aaaaggtttc cgtcacatca tggtacattt cccgcatctt ctgaatatgt tcaatggtgc
     3301 accgatactg ggcctcccag tcaatgaaca gaacagagaa atgacgtttt ctcctgcggg
     3361 ccacctctgc cacaagatgg aaaagcacgg tggaatcctt tccacctgaa aaagacagac
     3421 ataccgacgg aaatgtttca aaaatccact caatgcgggc gattgcggca cgcaaaacat
     3481 tttctccggt aaggattttt tcggttaaaa gcgtatcact cattttcctg gctttatgtc
     3541 gaataattct gtttatacat tcaggcaaac ttcattaata actcaccgct ttggggataa
     3601 ataataacaa caaaaatctt cagatattaa cctggttgtt ggatttgtta tcacatcctt
     3661 tatccacctg cttcttactg gtaacacatc tggaaaatcc atgcattttc aacatatact
     3721 atcctttcaa cttgtaaata agaaaatatg atgcagatgg tgaacaattc aaatatcatt
     3781 ttgcaggaag tattcactgt aatagaatta ccccctgata aagtttattc actaaggaat
     3841 tattcaacag aaacattccc tgttagcttt gagcaatagc acttattttt atatgtatca
     3901 ggaatacgta cgttgatgta accaaaacaa agaagcctgt gtccatgggg ttcgtacaat
     3961 tgtgtgtggc acatgaccaa atcaggtttg agacctcccg ctgcaatatt ttttattcag
     4021 attttagcct gtcaggcctg acagaaacgc tcccaacttc ccttctatat cactgtctat
     4081 agtacgtaat cttagcaatg gcaggccgca tttcttcagt atactgtttt taagttcatc
     4141 acgttcagcc tgaacactgg tcagatgata acctccatcc acttcaataa cccctaatgg
     4201 agtcttcccg accttatagt aaacaacaaa gtcacaactg gcccggttac gcatgaataa
     4261 ctgttcacgg tgtgtaaaat ccccccgctc cagcaacacc aactgattca gcgctatttg
     4321 tgaatgaaac atcatactcc gataactatc ctgcgacagg atatcccgca acaggcaggc
     4381 cacaatctgt tctgacttaa agtgtgaatc atttgaattc aatctactat tcagtcgttc
     4441 cagtgatttg tcatactcac tgtacagcaa atcaaatgct gatatgaccg ggctctcgaa
     4501 aatctctccg tcatcagcgt agtatttgat gtaacgaatc agcgccgcaa tatgtccagc
     4561 gtggcgttca aatacatcat taccagtcac cagcgtaaat ttatttcggg cccttgaaac
     4621 agcgacattc accagttcag gattatcaac aaacgcgatg ttccgactgt gctggctgct
     4681 gcgcttctta tccagcacag tggaaaacac aatttcatcg cactcccgtc cctgaaattt
     4741 atgcaccgtc gatttcacaa aatcagcagg cagcactttt tcagccagat taacctgtgc
     4801 attatatggg gcaatgtatc ccctgctact ttcttcatcc cagtaatgac cttccagaga
     4861 ttcaagctcc ctgagatttg aaaaattacg ggtatggttt ccttttgccg tgatgaccag
     4921 agataatgag gcttcaccgg aatcaaccgt taacggtatc agagcattat cataaaactg
     4981 cttattacaa aattgaatga ttttaggatg acaacgataa tgctctttaa gaagggttac
     5041 tggcaccatg ttcctgaaca acatacaaac cgaatcaagt agactatatt tctcacaatt
     5101 atagtattct gccggtggag atgggctgtt cgggagtaat acaggaacat gtggaagttg
     5161 cttacgatcc cctacaacga ttacattacg ggcacatccc agtcccagaa tgcccggaac
     5221 aatatcctgc tgcgaggctt catcaataat aacataatca agaagggccc ccttgccgat
     5281 agagttaata atcgaatgcg tactgctacc aataatcgga aaacgcttta taaaacggtc
     5341 aaattcatcc ctgtatgtct ctgccgagaa tgaacttctt gttgaaacat ttcttcgcag
     5401 atgttgcttt aaataaagca tagaccatga tgtcaggcgc cccagtaaaa ttttaaaatc
     5461 agatttttta aatacttcct catattcatt cagagaaatt tgtttttccc ggatattttt
     5521 ttcataataa gaaagctgaa gagaagtaaa tagcgccagc cttctctccg gaatcatcaa
     5581 tggtttgact ttcagtatca tgaagtttaa caacaaatca attctgtttc tgaatccaat
     5641 ccgcctgtca gaaaggtaat gaatataagc catcaggtcg gttgtttttt gtgaggaaaa
     5701 tcggtatttg tgagaaaaaa gctcctccgg tcgaagattc tgactctgcc attgctgcag
     5761 gtatttatac tcaatattca gctcattaat ttctgctttt aacgaagcga cctggttaat
     5821 ggcattcagg tgttttttca cttgttgaag tacatcatca atcgcatttg cagacggtga
     5881 gtcaggtaag acttcctcag aacgcgaaat cgacgttgaa aaaaactgtt gcctgttctc
     5941 cgtactcccc agtcgtgcca tcacatatcc cagttgctgt ttgtccatct tttcataaac
     6001 attactgaca gctgagttat tattggacaa aattgccact gttttattct gaattaatat
     6061 attagcaaca atattgagta tagtctgtgt tttacctgtt ccaggtggtc cttcaataac
     6121 tgaaatctgt gaggaaaatg cccgtttgac agccagcaac tgactttcat taagaccaaa
     6181 cggaaaaatc aaaccccatg gcgaatcacg ttttttactc tgtcctgaac agtatgcatg
     6241 caaggcggta tccggatgag gaactatttt ctttatctgc ctaaggatat tctcatcgat
     6301 atttctgtta ttaccacggg cataaaaaat acgttcatta gccaccctac acaagtaatt
     6361 aaacacggca tgctctgtaa atgctgaaca ctgttgaaag tcaaggccgg tcgttttcat
     6421 gatgtagggt cgttcaccct cgcggtaata aacagcagca tatttatcac catatataat
     6481 aaccagttca gcccgggatg ttacagcgtt ccctttcctt tcgaggaaaa catttcgcaa
     6541 atttacagtt ggcgttatct gacatacaga aagcggacga gtatattttc tcccgctgcg
     6601 aaaatgacag gtcagcataa cctctctgtc gtcatttcca gcccacagcg tccagtcggt
     6661 aattttatga gtcttatttt caccgtcaac acaaatcgtg accatattct cactcagaaa
     6721 ttatcattta acttacggaa aattgttaag gcactcccga tattagctcc ggaggatttt
     6781 ccacaactcc gggactgtta acatatttct gcctgccata tatacattga atttagtcat
     6841 aacagtcaat accagaatta caggaatgcg atgccgatac gctggttatt gaatactggt
     6901 agcccctgag cactaattca tcacgatact ctgtatttac tgttttcact gctttttaca
     6961 accattcccc caggtaaaat gctgctcctc cggtgatggc tgtgtcagaa actgccatcc
     7021 cggaatagcc acgctgaccg ccagcaatgc cgcacagaca ccagccacaa acggtttcag
     7081 aaacgtgggc ggggatttct gaagggaggg tgactgctcc gcctgcactt tccccttgcg
     7141 attatcaagg ctcagtgcat ccagacgcac cgcgccattt gccgcaagca gcccttcccg
     7201 gttatccacg cttcccggca gactcagcga aaaatcccct gttcccgtct gaatcagact
     7261 gcctttacga tttgacaggc tttgtgccga tatctgcagt gtgtcggcat ttatcgtacc
     7321 gccattactg ctgaactgcc cggcagtccg ggcggaaagc gtaccggcac tcagttgcgc
     7381 attctgtgta gacagatttc cactggttgc attcagctca gtatgttgtc cctgtatccg
     7441 gctgttacct aaatccagcc cctgctcaat gtcccgtaaa tccagcagag aaccttcgtg
     7501 tgccgggaat gaggaataca actgaatata gtcatcactg tccggtgtca gacgcacatg
     7561 tcgcacaaca ccgggaataa tgaccagccg gaggataccg ctttttaaat cctgcgaagg
     7621 tgccagaaca cgggtggtga cataaccatg atcgaccaga cggttctgca atgtactcat
     7681 cagcagatta attcctttcg cccccaggca atgcccgacc gccccgttgg cgattttttg
     7741 taaaggcagc cagtgtggta acgcatcagc ccccttcagt tccgtctgtt taatctgaaa
     7801 acagggcgtt tcaacaggaa aattaatctt atgggcaaaa gagccaggtg cagaaaggcg
     7861 aacatcaggg gctgaaggcg tcagttgttg ctcaagggct tcctgacgct gctgctgaat
     7921 gataaatgct tcatctccgc tacgccggac atccgctgcc tgaagagata aaggcgataa
     7981 taaagcagac atcagtgtca gtgacagcaa ggacggacag cgccggaata agctttgttc
     8041 ctcagtcctc tcgttcctgc aacataattt tccagtcaaa cgaaaaacct tttattcatg
     8101 tcataccaac aatcaccagc ctcctgacga tacctcccac catgttttgc gatggcacgc
     8161 taccgccctt ccggtattta cccgcatcaa ccggtaccag aatgctgagt aacaaaccag
     8221 ccagaattga cttaattttg atcattatag aataatgatc tgaactacag caacatgaca
     8281 gatttatcga tcgatataac acaaatgaac agttctttat tctgcaatat atcgcaaaaa
     8341 cagcacatta caaaatttat aaccataatt actggaaaag tgcagaaaat tattccactg
     8401 aatgctttta atacagttgg ttagcttaaa aaatagcaat ttttgatttc agtcattgaa
     8461 agttgcaatg tttgacgatt caacagtatt taagtgagtt tcaggatatg ggaaggacat
     8521 gaaaggagga gcgcaataaa atacacagga agaaggggaa aatgtttcgt aaaaacagaa
     8581 aattaacagc gcacatcagg aaacgcagtc tcctgattca gtgctaccac atgaaaattt
     8641 agttaactgt taccgtacta aaatttatgt cagaagatgt tccattaccg aaagtcaatc
     8701 agcgatacaa agacgatcat ggtgcgcttg tcaccctgac cagtgttgag gaaacccggg
     8761 tggtgtttat gcgggacggc tatccgcatc cctgcatgca accgatgtac aactttctgg
     8821 gaaaattcaa acccgaaccc cgggaagaaa cagaataaaa cgaaatccct gccggcaatc
     8881 acactacctg atagcagcat aatccactta ttaattctca tccccggaga caagcccaga
     8941 aaaccactcc gggtgttcac tgtatgccgt gttcagcgca tccctggaaa cgtttatata
     9001 aaagctgtcc tgcaatttgc gggtaacaac ttctgccagg cgattccggt tatccaccag
     9061 tccggcataa cctctctctg ccagccactc attcagttca tgttccagag acagattgat
     9121 aatatcataa tcggaccttg ccattcccgt ttccttagca catgttttca ggcacgggga
     9181 taaagtataa agaatgtcat actgctgctg ccagagcaat attccccctg atggttgtat
     9241 cagagatggt atgccggtac tttgcgggtt gttcccgtca ggttatcgtg cacctccacg
     9301 gtcgtggtca ccaccggcat tccggccttt ctcagcctca gaacatcagc cgcaatacgc
     9361 tgactgccaa accagaacag gccgtccagt gcagtcacca gcaaacccgc ctccagtgca
     9421 tacttcagcc gttcacgggg cgctttcact tcccgggcaa cctgctgata tggcaatgat
     9481 gtgttttcat tcccaaccac gcgacgaata cggtgagaca gatgataccg gtatgtatcc
     9541 ggcacaccag aaaggctggc cttcaggctg tacacgcagc caaaccgttt atcgttaaac
     9601 accacatttt tctggctgat gccccattct tcacgcagcg cggcaatcaa ttgcggtgta
     9661 cgggtaagca gcaagcgaaa aggcagttca aaactggtga cgtaatccac attcagcagt
     9721 gcaatacgca accgttcttc tggcccggcc tctgtctgcc ggcactcctc cagaacatcc
     9781 tgccactgcc ggcgaagacg gggggattcg ttccgttctg taaaacagta tttatccgcc
     9841 agatagtcaa ttcgtgtacg catactgaaa ggaattccgt ataaagattc agcagacaaa
     9901 actttatcag ccagtaaaaa ttaacggaag cattgatatt cctcccgaca atcaccggat
     9961 gattgttgca ataacctgcg acataaagga ctgaacagcc gtttttaacg ccacgtattg
    10021 ccctgatgaa ccaggccggg caacccgaaa acatctttcc acccggcatt gtccgccagc
    10081 acttatcacc gaccaggtct gttgcagtaa atccaccagt tgcgcacatt cttcatcagc
    10141 catgacgccg actcgcggat ggcaaatact cgtctggctg agcagcaaca gacatcgtat
    10201 ctgtgctcct tcatgttgtt cccgcaacca gcgtaatacc accggcgctg atgggctgac
    10261 cgtgtgatta tgctccgtaa tacgttcgtg tacccgtcgg tgaaaggaac tacaaaatgt
    10321 ctgaatctgt tgaccataga ttccttctgc cgaatagagc gtgaagtgaa atgccaacag
    10381 atttggccag tggtccaccg cctgctgaac aaaacgccgg atttcccccg gctctgaaat
    10441 taaggcttcg gttatttgca cgaaatgttc cttttcaacc cctgttgaat tttgtcaagc
    10501 cggtgcagac acagtaaagc gcgcatcaac acctgaaagg tacgatacaa acacaagtta
    10561 caaacaataa gaacggaagg acaattaatc agtgaggaat aataactaaa ttaaccaaaa
    10621 gaatgttact gacaatacta ccaatactta aatagcaata tattaattaa tgtaagaaca
    10681 gatgcccggt aagacatata cacaatacat ccacaaacac actcttttca ttaaatcaat
    10741 cacctgatta aaccatgaaa gtgtgatgta agtcaaaatt actgatacta tcaattgata
    10801 atgattatct ctatcattat cgtgaaattc gagaatgaaa ggagaagtag tcattataag
    10861 ccttttataa atcgtcatca tcatagtaaa gtagaacact ttttatatga gttactatat
    10921 gaaaatatac tggacaagaa aacgtattcc tgaactttca gcacttccac cttcattaag
    10981 aaagaaaaat tttactgacg catataatgc agcatcttct catatcgaat actggatagg
    11041 tgctggcgtc tcatttatct cgatgatgat tttatccaga gtatatgact ttcttttacc
    11101 cgcccaagat actttcccgg gtgatatcat cagaagttta tgtgttgtgt gtccatccat
    11161 attaatctgg tttcagtttt cggtatatgt catgaggaaa tattacagac atattctggt
    11221 gcgaggaaaa gaaaccgaaa ccatttcaga aaggttgata cgtgaggctg atacacgaga
    11281 atatgaacta tggcgtccgg tccgtcgctt tttctccatt gttttcctca ttgttctatt
    11341 aggttgcatt cattcactga taacaactat aaaataaaag ttatggctgt tctgatacag
    11401 gaaaataaac gttcattcta tggttacctc ttcctgttat atagaagaat tttaaacgac
    11461 aagtaaggga atataaatga aaataacaaa aaataaaaat gcaactataa aaaacagtca
    11521 tcagtcggct gcttttcaat gtttacctta tcatttt
//
LOCUS       DAJTXY010000120        11535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTXY010000120 DAJTXY010000000
VERSION     DAJTXY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.120"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            8..451
                     /locus_tag="OO566_003346"
     CDS             8..451
                     /locus_tag="OO566_003346"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004133821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6606518.1"
                     /translation="MQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQIDES
                     RWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIKSES
                     ELTVDASITAKPFFERYVFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            697..1741
                     /locus_tag="OO566_003347"
                     /pseudo
     CDS             697..1741
                     /locus_tag="OO566_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1803..2417
                     /locus_tag="OO566_003348"
                     /pseudo
     CDS             1803..2417
                     /locus_tag="OO566_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2474..3931)
                     /gene="pepD"
                     /locus_tag="OO566_003349"
     CDS             complement(2474..3931)
                     /gene="pepD"
                     /locus_tag="OO566_003349"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6606519.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4193..4651
                     /gene="gpt"
                     /locus_tag="OO566_003350"
     CDS             4193..4651
                     /gene="gpt"
                     /locus_tag="OO566_003350"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6606520.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4743..5987
                     /gene="frsA"
                     /locus_tag="OO566_003351"
     CDS             4743..5987
                     /gene="frsA"
                     /locus_tag="OO566_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6606521.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6045..6446
                     /gene="crl"
                     /locus_tag="OO566_003352"
     CDS             6045..6446
                     /gene="crl"
                     /locus_tag="OO566_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6606522.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6485..7540)
                     /gene="phoE"
                     /locus_tag="OO566_003353"
     CDS             complement(6485..7540)
                     /gene="phoE"
                     /locus_tag="OO566_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6606523.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7828..8931
                     /gene="proB"
                     /locus_tag="OO566_003354"
     CDS             7828..8931
                     /gene="proB"
                     /locus_tag="OO566_003354"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6606524.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8943..10196
                     /gene="proA"
                     /locus_tag="OO566_003355"
     CDS             8943..10196
                     /gene="proA"
                     /locus_tag="OO566_003355"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6606525.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10311..10386
                     /locus_tag="OO566_003356"
     tRNA            10311..10386
                     /locus_tag="OO566_003356"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10344..10346,aa:Thr,seq:cgt)
     gene            complement(10401..11429)
                     /locus_tag="OO566_003357"
                     /pseudo
     CDS             complement(10401..11429)
                     /locus_tag="OO566_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2764 a         2861 c         2972 g         2938 t
ORIGIN      
        1 gaataacata caaataagag attatcagcc tggcgatttt cagcaactat gcgctatttt
       61 cattagagcg gttacgatga ccgtcagtca gcattattca ccacaacaaa ttgccgcctg
      121 ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag tgcgggttgc
      181 agtcattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata tcgatatgtt
      241 atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa aaccttttat
      301 taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct tttttgaacg
      361 ttatgttttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt ggtttattaa
      421 tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa taacgcccgc
      481 ctagtatgtg cgcgttttct tatttcacaa actgcaagga ggtaaatcat gacaaatcct
      541 ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg ccctggattc
      601 gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc agcacatgca
      661 acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat tcgtccctta
      721 tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt agcgatccaa
      781 caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat gccagattta
      841 caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg tttttaccca
      901 gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga tattctcgct
      961 cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga cgttgctgaa
     1021 gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg gcgcagctcg
     1081 cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga tcaaatcatc
     1141 aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact gctggttcat
     1201 agcggctcga gggagctagg gcagtctatt ttacagcggc atattgcctc gttttcgcat
     1261 catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa tcgccacctg
     1321 atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct ggatgtggcg
     1381 cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca tcgtaaaggt
     1441 gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga ttactccagg
     1501 ctggttcagg cccgtcagga gtgaggaaac attgcattcg ctggcgcatg gggctgggcg
     1561 taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag cgacgcaact
     1621 ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca tcttcgaaga
     1681 agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtctgggca ggccggatta
     1741 attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg agggaaaaaa
     1801 ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt ctcgcagtga
     1861 aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg gtaacggtgc
     1921 tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt tctctggatg
     1981 gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtgg atttgcccga
     2041 gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg cgttttaccg
     2101 ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg tcggggccgg
     2161 gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg gcatccggta
     2221 ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg gcgcgattgc
     2281 tgattgcctg gaagctggat cagcagcaat aggaaaatag cgcggtgctg aaatcacagc
     2341 ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt acagggatgg
     2401 cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg ccatccggca
     2461 gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc agtgtccagt
     2521 aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg gtgatagttg
     2581 gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca cattccaggc
     2641 ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag gtttcacgta
     2701 ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg cctttcgctt
     2761 cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg tagtctttac
     2821 cgctgtcgat cagtgaacgg atcaggcagt gaatttctac gttattgtca gtcatggtca
     2881 ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa ttgcggatca
     2941 caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc gcaatcaggg
     3001 cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct ttctctgcca
     3061 gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg tcgactttat
     3121 cagctgtgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc agtgtgccgc
     3181 cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg aagcgcacca
     3241 gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga ccgcctttca
     3301 gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca cgatctaaat
     3361 gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca ccttcttctt
     3421 cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag ccgaacgcac
     3481 cgtccatacc ggcttcttcg gttatggtca gcagcacttc cagcgggccg tgaaccacgt
     3541 tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc gcacccagcg
     3601 tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga tctttcgtga
     3661 agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc tgtaagacga
     3721 ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata ttacctacct
     3781 gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat tcagcgagtt
     3841 gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata tcccacagcg
     3901 gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat cacccgcaaa
     3961 acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca ggcttgcgag
     4021 atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
     4081 ggactcatgt gaaatactgg tttttagtgc gccagatatc tataatctcg cgcaacctat
     4141 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
     4201 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
     4261 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tggtaccggg
     4321 ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     4381 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     4441 cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga ttcgtgaaat
     4501 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     4561 tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg atatgggcgt
     4621 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat tgccgggcgt
     4681 tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg gaggctgcat
     4741 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     4801 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggctcttg
     4861 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     4921 gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgctgaac
     4981 gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac tggatttatg
     5041 agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa gatccgcaac
     5101 tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc tatcctcatc
     5161 tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc tatgaagagg
     5221 ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc ggcggtgcgc
     5281 ccatcaccgg ctttttgcat atgccgcaag gcgatggccc gttcccgaca gtattaatgt
     5341 gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt tattttgcgc
     5401 cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct tcaaaatgga
     5461 agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg cctaacgtac
     5521 cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct aacgttgccg
     5581 tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt ggtccggtag
     5641 ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg tatcttgacg
     5701 ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc gtggagctga
     5761 atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg ccaatgttat
     5821 caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta atcacctcat
     5881 catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg aattttgaca
     5941 aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa tttgctaaat
     6001 tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg ttaccgagtg
     6061 gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat attcgtgaag
     6121 gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc aacgtgaaac
     6181 cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg caggaatccc
     6241 gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg aagagtgttc
     6301 cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag tttcacgaga
     6361 agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcggat gattttcgtg
     6421 atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca tccggcaata
     6481 ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta atattcagct
     6541 tattatcgct atccagtttg ttgattttat aatcaacaaa cgctgacata tttttgttga
     6601 aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg atgccctcaa
     6661 tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag tcaaactggt
     6721 attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt attggcgtca
     6781 ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg tatttcagac
     6841 ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc tgctcgttgg
     6901 tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca aagtcatatg
     6961 tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt tcgtttttcc
     7021 cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg gtgttccgat
     7081 acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc gaggaatcgc
     7141 caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg cccaggttac
     7201 gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg agacgcgttt
     7261 tttgctgtgc agtatcactc tccgctttat ttccggcaaa ctccgcttcc caccgaccat
     7321 aaccagtcag ttgatcgtta atttgagttt cacctttaaa accaaaacgg atataactct
     7381 ggtcgccatc tttactggcg ttatcactca tataatgcat ggctttaact ttgccataga
     7441 catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat gcagatgcca
     7501 caatgcccat caccactaat gccagagtgc tctttttcat tttcattcct gattttaatt
     7561 aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt aacctgagat
     7621 tgttaaagat atattacaga ttaatgatat tctaaaaatg tggtaattta ttaaatctgt
     7681 aataaaagcg taaacaactg ccgctacgct tgctgatccc gcgcaacaaa acgccatgct
     7741 ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt tgatatcatt
     7801 tttcctaaaa ttgaatggca gagaatcatg agtgacagcc agacgctggt ggtaaaactc
     7861 ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat cgttgaactt
     7921 gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt gacgtcgggc
     7981 gcgatcgccg ccggacgtga gcacctgggt tacccggaac tgccagcgac tatcgcctcg
     8041 aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga acagctgttt
     8101 tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat ggaagaccgt
     8161 gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgatcacaa tatcgttccg
     8221 gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga taacgataac
     8281 ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct gaccgatcaa
     8341 aaaggtttgt ataccgctga tccgcgcagc aatccgcagg cagaactgat taaagatgtt
     8401 tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg cctcggaact
     8461 ggcggcatga gtactaaatt gcaggccgct gacgtggctt gccgtgcggg tatcgacacc
     8521 attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg catttccgtc
     8581 ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg gattttcggt
     8641 gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat tctggaacgc
     8701 ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc gcgtggtgaa
     8761 gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc acggtgtcag tcgttacaac
     8821 agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc aatactggga
     8881 tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta aggagctggc
     8941 cgatgctgga acaaatgggc attgccgcga agcaagcctc gtataaatta gcgcaactct
     9001 ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa gcacaaagcg
     9061 aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat ggccttagcg
     9121 aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt gccgacgatg
     9181 tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc ggcgtactgg
     9241 acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc gtgatttatg
     9301 aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc ggtaatgcgg
     9361 tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc ggcaacggtg gcggtgattc
     9421 aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt gcaggcgatt gataatcctg
     9481 accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg ctgatcccgc
     9541 gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg gtgatcacag
     9601 gtggtatagg cgtatgccat atttacgttg atgaaagcgc agagatcgct gaagcattaa
     9661 aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg taatacggtt gaaacgttgc
     9721 tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa atggcggaaa
     9781 gcggcgtgac attacacgca gatgcagctg cgctgacaca gttgcaggca ggccctgcga
     9841 aggtggtggc ggttaaagcc gaagagtatg acgatgagtt tctgtcatta gatttgaacg
     9901 tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac ggcacacaac
     9961 actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt aacgaagtgg
    10021 attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc cagtttggtc
    10081 tgggtgcgga agtggcggta agcacacaaa aactccacgc gcgtggccca atggggctgg
    10141 aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt gcgtaaataa
    10201 aaccgggtga tgcaaaagta gccatttgat tcacaaggcc attgacgcat cgcccggtta
    10261 gttttaacct tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg gccgatatag
    10321 ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt aggttcgact cctattatcg
    10381 gcaccattta aatcaataag ttacctcgca tttaagtaaa ccacgttctc ctcttgtgcc
    10441 gtatttgtgc cattgcgact tataatcgca tcgattttgc ttgcgtgctc ggtgagatgc
    10501 ccggctgaaa ggtgggcgta tctttgaacc atttcgagag tttcccatcc tcccatctct
    10561 ttaagtgcaa gaagagagac accggactga accagccagc ttgcccaggt atgcctcaga
    10621 tcatggaagc ggaagttgct aatgcctgcc cgctttaacg ctcccttcca tgccttgttg
    10681 ctgtcggttc tcatcttcct taccgctgct gtttttgttc cgtcgcttcg gtaggcaggt
    10741 ttggtgtgga caaataccca tctcttatgg agcccctgct gttttcttaa tatctggcat
    10801 gcggtttcgt taagaggaac tccgatcgca ttgccagctt ttgtttcatc agggtgcatc
    10861 catgccattt tcttatccag atcgacctgt gaccactcaa ggtctgtaac gttggaacgg
    10921 cgaaggcctg tcgtgattgc aaacatgacc acagggaaga agtgaggagc aatttctgca
    10981 aacaggcgct tcgattcctc ctctgtaagc catctgatgc gtccattctt aacgcgtggt
    11041 gttgatattt tgggtgccct gtcaagccat ccccattcaa cagccatatt gagaatggcg
    11101 cgaagtattg ccagatgcct cgtcttcgtt cctttgcttg caagctttgg tttatactcc
    11161 ggcaccggat tgccaagtcg caaacacctg tcccggctca tctcccagtt caggcgatgg
    11221 cggcggtttt ccatcccgtc taccgcctcc attatttttt ctgttgttat gtcagagaga
    11281 atggcttctc tgaagtgcaa catccagaac gatataatgc tcttgtcatc atcaatggac
    11341 ttcttatccg atttctcacg cagccaccgt atgcaggctt ccttgaatag ctttttcggt
    11401 gattccccga gattttttac tctccacgct ttgaagtggt caacaaaaac tggccaccga
    11461 gttagagttt ttccagtatc gattttccga ttcgtttggg ggtaacccac cgttatattc
    11521 gtgcggtctt agtgc
//
LOCUS       DAJTXY010000121        11386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTXY010000121 DAJTXY010000000
VERSION     DAJTXY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.121"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="OO566_003358"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="OO566_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6606526.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTVLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="OO566_003359"
     CDS             complement(2005..2928)
                     /locus_tag="OO566_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6606527.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGHAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINTIRKSMPF"
     gene            3145..4488
                     /locus_tag="OO566_003360"
                     /pseudo
     CDS             3145..4488
                     /locus_tag="OO566_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="OO566_003361"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="OO566_003361"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6606528.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTLQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="OO566_003362"
     CDS             6508..6732
                     /locus_tag="OO566_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6606529.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="OO566_003363"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="OO566_003363"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6606530.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /locus_tag="OO566_003364"
     CDS             complement(7326..8306)
                     /locus_tag="OO566_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR6606531.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="OO566_003365"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="OO566_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6606532.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="OO566_003366"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="OO566_003366"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6606533.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10983
                     /locus_tag="OO566_003367"
     CDS             10315..10983
                     /locus_tag="OO566_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6606534.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11001..11386)
                     /gene="tnpA"
                     /locus_tag="OO566_003368"
                     /pseudo
     CDS             complement(11001..11386)
                     /gene="tnpA"
                     /locus_tag="OO566_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2937 a         2658 c         2856 g         2935 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg tgttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cctcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggccgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatacag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag tgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agcagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagact
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgt gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgtaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg ctgttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcactag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaagaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgctgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcagg
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact cgcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cccgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga ggaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgcgcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatattct ggttttttaa acaattactc tgatttaaaa
    10441 gaaacaacct cagcaacagg taaacctgtt ttacgttggg tagacccgag ttttgatcaa
    10501 agcaaatatg acagcatcgt ctggaaccca atcacttatt atccggtacc aaaaccttct
    10561 acccaggtag ggcagaaagt tctggataaa attttgaact ataccaacac cgaaatgaaa
    10621 gaagcgatag cgcagcgtaa accagtggtt accaccgctg ggccgcgtag tctgattttc
    10681 cgtggggcca ttaccggtgt agataccagc aaagaagggc tgcaattcta tgaagtggtt
    10741 cctgttgcat tagtggttgc ggggacgcaa atggctacag gccaccgtac catggatact
    10801 cgcctctatt ttgaaggtga gctgattgat gcagcgacta ataaaccggt tatcaaagtc
    10861 gttcgtcagg gcgaaggtaa agacctgaat aacgaaagta cgccaatggc tttcgaaaat
    10921 attaaacaag ttattgatga catggcgacc gatgccacca tgtttgacgt taacaaaaag
    10981 tagttccaga cgcgccatcg ttagatggcg ctttttatcc ggtgcgccgt aaaaccccat
    11041 ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgtttg ctgttgttca
    11101 atgtattggc ggatgacgga tattggcgca cctccgcagc tactggcaaa atagccagga
    11161 ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt
    11221 aacctacccg atacgccttt gaggctgttt accagactgg atatcgccag tttgggaggt
    11281 tagttaatta acaaatggac gtgatctggt tcgccatcca tttcaaccag ttcagcttca
    11341 aaataagcac atacatttga aaagtaagtg cgtagttttt ctgtcg
//
LOCUS       DAJTXY010000122        11367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTXY010000122 DAJTXY010000000
VERSION     DAJTXY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.122"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..212
                     /gene="yfdR"
                     /locus_tag="OO566_003369"
     CDS             <1..212
                     /gene="yfdR"
                     /locus_tag="OO566_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6606535.1"
                     /translation="VMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVI
                     PLAPGHAYGMFMERFNELSELRKCA"
     gene            203..565
                     /locus_tag="OO566_003370"
     CDS             203..565
                     /locus_tag="OO566_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606536.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            565..870
                     /locus_tag="OO566_003371"
     CDS             565..870
                     /locus_tag="OO566_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606537.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGNNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            1097..2260
                     /locus_tag="OO566_003372"
     CDS             1097..2260
                     /locus_tag="OO566_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6606538.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVVTKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKESTNDV"
     gene            2610..2972
                     /locus_tag="OO566_003373"
     CDS             2610..2972
                     /locus_tag="OO566_003373"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCR6606539.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            2969..3886
                     /locus_tag="OO566_003374"
     CDS             2969..3886
                     /locus_tag="OO566_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6606540.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            3899..5164
                     /locus_tag="OO566_003375"
     CDS             3899..5164
                     /locus_tag="OO566_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR6606541.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(5231..5854)
                     /locus_tag="OO566_003376"
     CDS             complement(5231..5854)
                     /locus_tag="OO566_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6606542.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(6605..6943)
                     /locus_tag="OO566_003377"
                     /pseudo
     CDS             complement(6605..6943)
                     /locus_tag="OO566_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            7065..7325
                     /locus_tag="OO566_003378"
     CDS             7065..7325
                     /locus_tag="OO566_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCR6606543.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(7343..10078)
                     /locus_tag="OO566_003379"
                     /pseudo
     CDS             complement(7343..10078)
                     /locus_tag="OO566_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(10079..>11367)
                     /locus_tag="OO566_003380"
     CDS             complement(10079..>11367)
                     /locus_tag="OO566_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6606544.1"
                     /translation="GGVNSNAPNVNDIGNTLQLIRQNNDIERSGANNVGLTALQGLSG
                     IAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQIESVRKGMGFIDEDQ
                     RNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPQDVAQMYQQNPRQFGEFVD
                     HLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRI
                     NLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGI
                     NTLTTSMLTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQA
                     MRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNG
                     IKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
BASE COUNT         3073 a         2588 c         2522 g         3184 t
ORIGIN      
        1 cagttatgag tacgcccgtg aaatatgccg atctcatcat gctggcaacc gaacgccgcg
       61 atctcgggct tgatgatggc tctttctggc ctgtactgga aggtatcccg gcaacagaga
      121 tgttcaacgt gattccactg gcaccgggcc atgcctacgg gatgtttatg gaacgcttta
      181 acgagttatc ggagttacgc aaatgcgcat gaatgttttc gaaatggaag ggtttcttcg
      241 tgggagatgt gtaccgcgag atctgaaagt aaatgaaaca gatgctgaat acctggtgcg
      301 taaattcgat gcgcttgaag ctaaatgtgc agcacaggaa aacaaagtaa taccagtgtc
      361 aactgaactg ccaccagcaa atgaaagtgt tttgttattc gatgctaacg gagaaggctg
      421 gctaattggt tggcgttctc tctggtacac ctggggacaa aaagaaaccg gagaatggca
      481 gtggacattt caggtcgggg accttgaaaa cgtcaatatc actcactggg cagtaatgcc
      541 aaaagcaccg gaggctggag cataatgacc acttttaccg acaaagaact gattaaagaa
      601 attaaagagc gtatcagcag ccttgacgtg cgagacgata ttgagcgccg tgcttatgaa
      661 atcgcactcc tatctctgga agtagaacca gatgaacgcg aagcttatga attattcatg
      721 gaaaagcgtt tcggtgactt agtagatcgt cggagagcaa aaaacggcaa taacgaatac
      781 atggcatggg atatgactct cggttggatc gtctggcagc aacgagctgg tatccatttt
      841 tcaacaatgt cacagcaaga ggtgaaataa tggagccata cagcctcaca ctcgatgagg
      901 cctgtcattt tctcaagata tccagaccga ctgccattaa ctggatacgc acagggcgtc
      961 ttcaggcaac acgcaaagat cccactaaga ataaatctcc ttacctcaca acacgacaag
     1021 cctgcattgc ggctcttcag tctccgctgc atactgtcca ggtgagcgcg ggtgatggca
     1081 taacagagga aagaaaatgt cactcttccg cagaggtgaa atatggtacg ccagtttcac
     1141 attgccgaac ggtaaaagat ttaaacagtc tcttggaaca aaggacaaaa ggcaggcgac
     1201 agaactccat gacaagctaa aggctgaagc atggcgggtc agcaaacttg gtgaaatacc
     1261 tgatataacg ttcgaggaag cgtgtgtcag gtggcttgaa gagaaagcac ataaaaaatc
     1321 actggacgat gacaaaagcc ggatcggatt ctggcttcaa catttcgcag gaatgcaact
     1381 aagagacatt actgaatcaa aaatttattc agcaatgcag aaaatgacga accggcgtca
     1441 tgaggaaaac tggaaactca gggcagaagc atgcagaaaa aaagggaaac ctgttccaga
     1501 atacacgcca aaaccagcgt ccgttgtaac gaaggctacg catctttcat ttataaaggc
     1561 cctactaaga gccgcagagc gtgaatggaa aatgctggat aaggcaccaa ttattaaagt
     1621 gcctcaacca aagaataaac ggatccgctg gctggagccc catgaagcac aaaggctgat
     1681 tgatgaatgt ccggagccat taaagtctgt tgttgaattt gcactggcaa caggcttaag
     1741 acgctcgaac atcatcaacc ttgaatggca acaaatagat atgcagcgcc gggtggcatg
     1801 gataaacccg gaagagagta aatcaaaccg cgcaatcggc gttgcgctga atgatactgc
     1861 atgtcgcgtt ttgaaaaaac aaatcggaaa tcatcaccgt tgggtatttg tgtacaagga
     1921 aagctgtacc aaaccagacg gaacgaaagc gccaacagta aggaagatgc ggtatgacgc
     1981 aaacacagcc tggaaagcgg cgctgagacg ggctggtatt gatgatttca gatttcacga
     2041 cttgagacac acctgggcaa gttggctggt tcaagccgga gtcccgttgt cagtgttaca
     2101 ggaaatggga ggctgggagt ctatcgaaat ggttcgtcga tatgctcacc ttgcacctaa
     2161 tcaccttacc gaacacgcac ggcaaataga ctcgatcctg aacccatcgg tcccaaattt
     2221 gtcccagtca aaaaataagg aaagtactaa tgatgtgtaa cttattgatt taaatggtgc
     2281 cgataatagg agtcgaacct acgaccttcg cattacgaat ctgtagcacc aatcataact
     2341 atctgtttta gcaagcatta accgcattca ctaagatata gttgatggca caaacagaaa
     2401 gttgatgaat gatgttgcca tatatatgtc acaaatacgg cacaacgatc ttcaaacata
     2461 tagccacaca ttcacagaag agcacaaagc cttgcaatcc agtgcaaaga tttgtgtgcc
     2521 agagtttttc tcatcaacta ccgcaagtat cgatcgattg agacttggat gatagacttc
     2581 atgcctttga ttattagctg atagaagaaa tgttaaagct atttgcaaag tacacatcta
     2641 taggcgtgct gaacacactt atacactggg tggtttttgg tgtatgtatc tatgccgcgc
     2701 ataccaatca ggctatggca aacttcgcag gtttcgttgt ggctgtgagt tttagcttct
     2761 ttgcgaatgc aaaattcaca ttcaaagcat caactacaac gatgcgctac atgttatatg
     2821 tcgggttcat gggaacactg agtgctactg ttggatgggt tgctgataga tgttcacttc
     2881 ctccaattgt cactcttgtt accttctccg ccatcagctt ggtgtgcggt ttcgtctatt
     2941 caaagttcat tgtctttagg gatgcgaaat gaagatatct cttgtcgttc ctgtcttcaa
     3001 tgaagaagaa gcgataccaa ttttttataa aacggtacgt gaattcgaag agttgaagcc
     3061 atatgaagta gaaattgttt tcataaatga cggaagcaaa gacgctacgg agtcaatcat
     3121 taacgctctg gctgtttctg atccgctagt tgttccgctg tcatttacac gcaactttgg
     3181 taaagaacca gcattgtttg cagggttaga ccatacaacc ggcgatgccg tgattccaat
     3241 tgatgttgac ctgcaagacc cgattgaggt tattcctcat cttattgaaa agtggcaggc
     3301 aggtgctgac atggtgcttg ctaaacgttc agaccgctca actgatggac gactgaaacg
     3361 taagacagct gagtggttct ataaattaca caacaaaata agcaccccaa aaatagagga
     3421 aaatgtcgga gattttcgac tcatgtctcg tgaggttgtg gagaacatta aactattgcc
     3481 tgagcgcaat cttttcatga aaggcatact gagctgggtt ggtggtcaga cagatgtcgt
     3541 tgaatatgta cgcacagagc gtgttgctgg catctcaaaa tttaatggct ggaaattatg
     3601 gaatctggca ctggaaggta tcacaagttt ttcaaccttt cctcttcgcg tatggactta
     3661 tataggcttg tttgttgcaa gcatttcatt tttatatggt gcatggatga ttatagacac
     3721 cattgtcttt ggtaacccag tacgcgggta tccctccatg cttgtatcaa tacttttcct
     3781 gggtggagtg caactgatcg ggattggtgt tctcggtgaa tatataggta gaatttattt
     3841 agaaacaaaa tcaagaccac ggtacttaat taaaagcaga aaataacagg atgatcttat
     3901 gaagatattc ataatgcgca attggcacaa gatatcaatt tttatacttg catttacttt
     3961 gatttggctt aggcgcattg acattttgac aaatgcccag ttttgggctg aggatgcagt
     4021 tttttggtat aaagatgcat acgaacaagg ttttttcagt tcactcataa ccccaagaaa
     4081 tggttatttt caaacagtat caacactcat tgttggtgcg acgacattta ttaatccaat
     4141 ttacgcacca ttattatcaa acttttttgg aataataatt agagctataa ttatatggtt
     4201 cttatttacc gatagattta aattcctaag caccacatca aaaatcttca tctctgtata
     4261 tttaatttgc atgcccgggc ttgatgaagt ccaagccaat ataacaaatg ctcactggta
     4321 tttatcattg tatgttgcaa tgataataat atctgaagaa tccaagtcaa aattatggaa
     4381 agcacatgat ttatttttta tagtattatc tggattgagc ggtccattta ttatattcat
     4441 aattgcatca tctgttttta aatatttaca cttatcaaaa ggagtaataa gcataagagg
     4501 tttatactta ttttatactc gactgccata tttggcaatg atcttgtgtg ggctgataca
     4561 ggcaacatca ataattctta catttaatgg aacaagatca catgctccat taggattcag
     4621 ttttgatgtt atgtcatcta taatatcatc taatgtattt cttttttcat ttgtcccttg
     4681 gaatatcgct aatgctggat gggataatca ccttttatcg tacacactat caactttagt
     4741 aattacatta tgtgttttca tttatattaa aggaaattgg caaatgaaag tgtttgccac
     4801 ccttccaata cttattgtcg cattttctat ggcaaaacct caactggcag attctgttcc
     4861 tcaactgcca acacttgcaa ccggaaacgg gtcaaggtat tttgtaaata ttcatattgc
     4921 aattttttca ttaatatgcg tttgtttgtt tcaatgcata aagaataaaa ccttaaaaat
     4981 attctttaaa atctatgttt cagtcatcct tattgcaatg ataaaattaa acttttttat
     5041 aacccctctt cctgacatga actggtctca aggagcagaa ttaataaata aagcaaaaca
     5101 tggagaagcc gtctcattta cagtgctccc tccatggtta actcttgatt taataaaaaa
     5161 ataaaacttc acattaataa ataacaatga atccgcctcc ctacgtattc ccgtagggag
     5221 gctgtcgtgt ttatagtagc ctggtaatgc taaatgcaaa cgaactggag attcctgctc
     5281 cgctaagcgt attgcgcagg gtcaccctaa atgtatctac accaatataa gtaacatcac
     5341 aactaactga atcaggcgca gatataggtg ttatctgaac cacataacca ttagtagctg
     5401 ctgcgagcgg atgagaaaac gttacatcat acacaccagt agcatgcgcg ttaacggatg
     5461 aaacacccgg cgcaatgtct acactaccat tggcgttaat tctccctgag cgcgtaactg
     5521 actctgcaga aaccgatgtg gccttactgg ttatttccat aaaggtgcaa ccaatatcga
     5581 tcaattcgca attgttccct gttatccagt atggtcgatc tgcacttggc tgataatccc
     5641 ctgcgctctg gaacgttgtg ccattgagta agactataac ttttccccca cctgagcttg
     5701 ttacttgtat attcgtgtgc gtgtagcgtt cactgctcac gcgatgaaaa tttccaccgt
     5761 ttataactac cgttacaggt cgcgtcccag tattatctat agcaaggtct gcccccccgg
     5821 cattagcctc gaagtatggg ttgtttaaca ccaaagcaca cgaaccgtta aggccattta
     5881 tggctaattg cgcaccacct gtaccggaat ccccctgagt accatttcct tcacaagtca
     5941 ggctgttgat agtagttcct gccccccatc tatcaccaag tataccccat cgtgtattct
     6001 ggcttgcagt taatcggtta actgtaatgg cattaagctc tgacccttca ctggagttaa
     6061 gtgcgcgcca gccaatacgg ttaccctgta agtacacatt atctaactct gacgttaaca
     6121 ccgagtctgt acgcaaccct tcgccggtag tgtgcccata agacacaatg ttctctaatt
     6181 tagtgtatgc tttattggtt acgagaagac cgtaagacga tcccgaacct ttaagtgtaa
     6241 tgtcccccag ataatcatag gcgtgtacac gttgggttgc cccgactgta tcaccgagca
     6301 tctttaagtt gaaatccgag gcatggcagt taaatatcgt ttctgccata ttttctccgg
     6361 aataatgagc acggactcct gtcgaaccaa taaatttagc gtcatagttg ccgtaatcaa
     6421 gaacgagtgg tgacgcgata tcgtacaccc cttgctttat ttttaatgtt ccaccgaaag
     6481 gccacgatgt aagcgggtct tgatatattt cattttttat tgcaaatagc tcctcttgga
     6541 gatttttgcc gctattagtg ccaatgtgag cagctccttc atctgattga agtaactgct
     6601 ttaactgatc aggatcgtac ttcagcacat tctgaaaata gaactgctgc gaaccatacg
     6661 catcatatac agccattgaa tggccttgct cagttacaaa tttggcaatc tgcccgttgt
     6721 acacaggata tccggcagca ttgataacaa ttggctgaga aacagggacg tgagatccgt
     6781 cttcattctc tacataaacc tgaatctggt tttcaggatt taccgggtca gtgtcaattt
     6841 ttccgatata aattttgcca ttggctacgg ctttaaaaga acgcgccata gtgaagagtt
     6901 gcgaaggcat gctgaccaca acatttgcgg cgatatctga catttcattg ctccagacga
     6961 atgatatgat gcaatcatga tgtgattgca taccgaaatg gcactattga atatttatcc
     7021 agtaggttac gatgccattc cacccaactg gtgaggcatc aaggatgtac agcaaatacg
     7081 acgaggcaca gtttcacttg agacttccgc atgaactcca cgcgaaaatt aaacagtgtg
     7141 cgaagatgaa taacagttcg ctgaactcag agataattgc agcgattgaa gaatcattgg
     7201 ctaaacaaag ctctgcatca gtttacattg acgatgcaga gcgtatggca gaacaacaat
     7261 ctgatatggt taagaaaatt gtctttgata cgctcaagga gctatataaa aaagacagca
     7321 actaaccatc cgtggctttc cgttacttct ggttatcatc actgttcatc cagtacatga
     7381 tgcctagcct tgatatggtt ttcttatctg attctggaag tgagttgtaa aacgagcgcc
     7441 aagcagaaga gcgtcttaca gttgtatcaa cgatgctttc tggtgcttgt tttactgcca
     7501 gccttgctgc tttctggaat tctggtgagc taattagctt ttctgcagat tcaattgatt
     7561 cagcgccgcc catcgccctg attttgctgg cgacttttgc cccaatttct gacccagcaa
     7621 cagcacctac tgcactgaac ggtcctgaca cagttgaacc aaccgcagtg ccaatgcgtt
     7681 gggcatggtt agcaacaaat tcatttgccg cagtgacgcg attaaaccgt ttgacgaact
     7741 cgtttagtct tcctgtagtt atctcgtaag attttgcatc tttgatagcc tttgcgacct
     7801 tatatacatc gttcagacct gacatagtct cttttggtaa atgtctggca agattgcgca
     7861 tctgcccgtt tgctaacatg ttttgatacc agtcagcaaa ccctgcagga ttaaaatcag
     7921 cgccacgttt tccgttcgaa agcatatccc gaagacctgt tcccagtagc tcggttctta
     7981 gcttcctgga cggcgtgttt tgcatcaatt cacgaaatcc tttggcatcg cctttcgaca
     8041 ttgcctgtag tgctgtagtt gctttgcgag aaacatcacc gttcagcgtt ctgccagtta
     8101 gattaatcat ttgctcttcc atgctttttc gcatctgaac aagcctctga gcaacttcaa
     8161 aatcacgtaa tgcatttgtc tctgacagcg ccgccttttg atcatcggcg agcgaaccgt
     8221 aaagcctcga taaagcagcg cgatcagcat ctttatatgg tccagaattc ttatgaagtg
     8281 catcaccaac aagtcgtcgt tgcttattga gatttgcata agttaatgcg ccgttcttgc
     8341 ctggattaac tgcccgaaag actctctttt caatagggtc aaggtttttc atcccgccaa
     8401 gatcttctgc cacctgtttg agcattgacc ttgttgatgg cgcatcaatt tttgcacctg
     8461 caggcatcgc cttttcaaca ttgcgataaa gctggtcgct tcgtttcatc agcgcagaca
     8521 ttcttccatt gaccgtatca ataaacttgt cgctcatacc aagagcatct tttgcaccgg
     8581 aaacgcgatc aattatctca cccgcgccct gtgctagctg cctgattgct tcattttcct
     8641 gcacctgtag agcagaaccc gcacgggact tgattgcttg ctcaactgcc ttgtattgct
     8701 cgttcccgga aaagtgtgaa gggagtaatg aatcaacatt aagcctttca gcagactcca
     8761 gaacttcagc ctgaggattg atatctagtt catcaagcga tgaagcgaga tttggccttt
     8821 tttgtgctgc tactgtgcga acgacttcct ctgggttctt cgtcgcaggc gtcattggta
     8881 caccttgtgc tactcttgcc tctgcggcat gaaccgcagg atttgttgct gcagcggcat
     8941 caccggagaa tggtgatgtt tctgttggcg cagtggtcgc tctctgtgca gatgttttat
     9001 tgccactaat tatatttgat gcaccacgaa caccacgggt aacaccattt atcaggccat
     9061 gcgtagcaat gctaagtccg gcattaatag cggcattttc ggcaaaatca ccccgttgat
     9121 tcgccgcgtc agcgagagaa ccaatgacca tgtttccagc tacacctgct ccaggaacaa
     9181 gataaccgcc tattgactct ccagcttgcg cgtaggggtc tgtcggtctg tctactggac
     9241 gataaacatc atccaatact tttggccccc caagcccctg actgattgca ttaatcagac
     9301 ttgcgccacc ctgcaatacg tcaaatggta tgtttaccag accacgacca gcctgttctg
     9361 caatttaccc tgcactttga ccaccagtga gccaatcgcc agcttgttgc atcaatgatg
     9421 gttcttcccg tgttggtgca ttattggcct gattaactgt ttgttgctga acaggtttcg
     9481 gtgcgacaga acttacgggt tgaggtggct gctgactggc tgcctgctgc tcaatctgag
     9541 caaatggatt atttgggtct gactgcacac ctgatgccga tacttgttcg gatgactgta
     9601 cttccagttg tgcaaacggg ttgttagggt cttgctgagg atgtaccttt gcagaggtgg
     9661 cgcgctgttc gactgttgag tctgtcaccg ggtcaccagc ccattgagca aagcgatcat
     9721 caacgtaacc gcggccttca ggtcctggcg tatattcacc acgctttgcc ttcataacgt
     9781 tgccgggacc gtcgtgataa gcctgaagag cgtcacgcca gttaccaaac tgctggtata
     9841 tctttgccag atagcgcgcg ccagcgtcag cctgatattc ggggttttgc atttgctcat
     9901 cggtataacc catatcacgc catgtcccag gcatgacctg agtcaatcct acagccccgg
     9961 cggagcttac tgcggcaggg ttgtaagaag actccttggc acccagtgca gtcatcaacc
    10021 cttctggcac accgtaacgt gcgccagcct gctctaacaa atcacggtaa ttagccattt
    10081 actgccccaa agatggaaga tatccgtagc gattaatgaa gtcgattgac agctcggggt
    10141 gctgcttcag gtaatctata gaagcctgag gcgcttccac tcgcttgata ccgttttgct
    10201 gaacgtactt accaaccgcc tcattacgct tctggttgag cgtgttcagg atgacgccag
    10261 cgttgcgacg aaaggactcc tcgctctgcg agttctgcag cgaaccaaca gcctggtcga
    10321 gctttttgcc ctcggcatca gaaagtgcgc ccatgcctcg catggcctga accgctgtca
    10381 ggtatgcttg ggatttaaag gtatcaagtc gtgcctgagt gtctgcagcc tgtgagcctg
    10441 gaacgttggg gattactcca cgtaagcctg taatgctctt aagtgaagga gaactaacga
    10501 tatcgttcag agttaacatg ctggttgtga gggtgttgat gccgtctttg tagccatcat
    10561 ttagcgcttg ctgcttctgc tgcaactgct ggttgttggc tgctatgcgg ctctgtattt
    10621 cctggcgctt caggtcgtta gtttctgctg atagcatccg gtcaaggcgc ttattttcgt
    10681 tgttaatgcg gtttgtttct gcgtccagat taatgcgctg ctgacctaaa ttcgcctgga
    10741 tatcttgccc gcgcattgtg attgcctgat tccgagcggc ggtttgcgaa tccaggtcct
    10801 gaccgcgcat ggtaacctgg cgaccctgca ttttatcctg taggtcaaag tatttttcgg
    10861 gtccgagact gttcatcccc aggtgatcga caaattcgcc gaactgccgc gggttctgtt
    10921 ggtacatctg agcgacgtcc tgaggattaa cgccaacacg agctaactca ccggcgttgt
    10981 tttgcagcca tgattgcatt gcttctggag acgatgacgc aaggcgtgcg ccagccgcta
    11041 aggtgccgat agaattacgc tggtcttcat caatgaatcc catgccttta cgaacggatt
    11101 caatctggtc tggatattga gtagccaact gacgcaaagc accgcgatca ccagacgcat
    11161 aagcattagc gtacgcctgc tgaaattctt tctgccgctg agcctgcttt tcctgctgaa
    11221 acacccccgc aatacctgaa aggccttgca aagcagtcag cccaacattg ttagcgcctg
    11281 aacgctcaat atcattgttc tgcctgataa gctgaagcgt attgccgatg tcatttacgt
    11341 tcggagcgtt tgagttgacg ccgccga
//
LOCUS       DAJTXY010000123        11245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTXY010000123 DAJTXY010000000
VERSION     DAJTXY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.123"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..489)
                     /locus_tag="OO566_003381"
     CDS             complement(<1..489)
                     /locus_tag="OO566_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6606545.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFSWRCDITSEQELSALADFAVSKLGKVDILVNNVGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSK"
     gene            complement(601..1629)
                     /gene="malI"
                     /locus_tag="OO566_003382"
     CDS             complement(601..1629)
                     /gene="malI"
                     /locus_tag="OO566_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6606546.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1804..3396
                     /gene="malX"
                     /locus_tag="OO566_003383"
     CDS             1804..3396
                     /gene="malX"
                     /locus_tag="OO566_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6606547.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3406..4578
                     /gene="malY"
                     /locus_tag="OO566_003384"
     CDS             3406..4578
                     /gene="malY"
                     /locus_tag="OO566_003384"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6606548.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4681..5682
                     /gene="add"
                     /locus_tag="OO566_003385"
     CDS             4681..5682
                     /gene="add"
                     /locus_tag="OO566_003385"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6606549.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5718..6758)
                     /locus_tag="OO566_003386"
     CDS             complement(5718..6758)
                     /locus_tag="OO566_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6606550.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7001..7126
                     /gene="blr"
                     /locus_tag="OO566_003387"
     CDS             7001..7126
                     /gene="blr"
                     /locus_tag="OO566_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6606551.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7399..7614
                     /gene="ydgT"
                     /locus_tag="OO566_003388"
     CDS             7399..7614
                     /gene="ydgT"
                     /locus_tag="OO566_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6606552.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7700..8140
                     /locus_tag="OO566_003389"
     CDS             7700..8140
                     /locus_tag="OO566_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6606553.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8217..8798
                     /gene="rsxA"
                     /locus_tag="OO566_003390"
     CDS             8217..8798
                     /gene="rsxA"
                     /locus_tag="OO566_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6606554.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFATIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8798..9376
                     /gene="rsxB"
                     /locus_tag="OO566_003391"
     CDS             8798..9376
                     /gene="rsxB"
                     /locus_tag="OO566_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6606555.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9369..>11245
                     /gene="rsxC"
                     /locus_tag="OO566_003392"
     CDS             9369..>11245
                     /gene="rsxC"
                     /locus_tag="OO566_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6606556.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AA"
BASE COUNT         2625 a         2907 c         2842 g         2871 t
ORIGIN      
        1 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
       61 ggtcagaata acgccaccgc catttttttc catttctggc gcaacaagtt gtgacagatg
      121 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      181 cggtttagga ccaccgccac cgacgttgtt aaccaggata tcaactttac ccagtttact
      241 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccagga
      301 aaatgcctga ccacccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      361 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      421 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      481 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      541 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      601 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      661 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      721 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      781 agaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      841 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
      901 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
      961 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1021 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1081 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1141 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1201 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1261 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1321 accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg tcaatccggc
     1381 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1441 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1501 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa gcaccagcga
     1561 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1621 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1681 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1741 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1801 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1861 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     1921 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     1981 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2041 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2101 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2161 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2221 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2281 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2341 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2401 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2461 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttacc gtttggtctg
     2521 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2581 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2641 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2701 ggcggtctgc caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2761 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2821 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     2881 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     2941 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3001 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3061 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3121 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3181 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3241 gaagatatgt cgcttgttaa tgtgcaagca ctgaaggaca atcgggcaat tggcgtagta
     3301 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3361 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3421 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3481 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3541 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3601 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3661 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3721 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3781 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3841 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     3901 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     3961 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4021 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4081 gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4141 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4201 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4261 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4321 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4381 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4441 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4501 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4561 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa tcgttgcgca atcgtggatt
     4621 tttaccctgc tttgttttta taatggtgcg cacttttata tccagaaaaa gagtgcgacc
     4681 atgattgata ccaccctgcc attaactgat atccatcgcc accttgatgg caacattcgt
     4741 ccccagacca ttcttgaact tggccgccag tataatatct cgcttcctgc acaatccctt
     4801 gaaacactga ttccccacgt tcaggtcatt gccaacgaac ccgatctggt gagctttctg
     4861 accaaacttg actggggcgt taaagttctc gcctctcttg atgcctgccg ccgcgtggca
     4921 tttgaaaaca ttgaagatgc agcccgtaac ggcctgcgct atgtcgagct gcgtttttca
     4981 ccaggctaca tggcaatggc acataagctg cctgtagcgg gtgttgtcga agcggtgatc
     5041 gatggcgtac gtgaaggttg ccgcaccttt ggtgtgcagg cgaagcttat cggcattatg
     5101 agccgaacct tcggcgaagc cgcctgtcag caagagctgg aggccttttt agcccaccgt
     5161 gaccagatta ccgcacttga tttagctggt gatgaacttg gattcccggg aagtctgttc
     5221 ctttctcact tcaaccgcgc gcgtgatgcg ggctggcata ttaccgtcca tgcaggcgaa
     5281 gctgccgggc cggaaagcat ctggcaggcg attcgtgaac tgggtgcgga gcgtattgga
     5341 catggcgtaa aagccattga agatcgggcg ctgatggatt ttctcgccga gcaacaaatt
     5401 ggtattgaat cctgtctgac ctccaatatt cagaccagca ccgtagcaga gctggctgca
     5461 catccgctga aaacgttcct tgagcatggc attcgtgcca gcattaacac tgacgatccc
     5521 ggcgtacagg gagtggatat cattcacgaa tataccgttg ccgcgccagc tgctgggtta
     5581 tcccgtgagc aaatccgcca ggcacagatt aatggtctgg aaatggcttt cctcagcgca
     5641 gaggaaaaac gcgcactgcg agaaaaagtc gccgcgaagt aacaaaaatg gatggtgcaa
     5701 attgcaccat ccatttttca cgcaaggcac aacgtcgcgc gatgtttggc ggattcgatg
     5761 cccagctcaa tcaactccat tacctggatt gcctggcttg ccggaaccgg attttcacca
     5821 tcgccattta acgcatcacg aatcgccgca tagtaagccg gataattccc aggcaccgtc
     5881 aacagcgttt cttcgacacg ttcctcacct tccacgcggg taagtacgcc atcacgcata
     5941 tcgtagcccc agtcttcctg cggcagacgc tcgccatttt tcagacgttc ttcctgtgga
     6001 tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg tgctgactca
     6061 gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga caagatggcg
     6121 tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac cgtcatgctg
     6181 accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa atcgtaccag
     6241 ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg acggaagcgg
     6301 tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag caaacctttt
     6361 agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac acgccccagg
     6421 ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa gggtttatca
     6481 acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg ggtatcgttg
     6541 ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt cggctcagag
     6601 acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct ggagattacc
     6661 gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt gctcgcataa
     6721 ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct cattgaagta
     6781 tgatggctat ttgacactat cctttaccca cgctcaacag tttaataacc tgccagcaat
     6841 aagggatgtt gtttaactta agtcaaaaaa atagcgaatt ttccaacgac aaaagctaaa
     6901 tatcgcaaaa acctcagtaa aaatcttgct ggagctatta ttgctaagta acatttaccc
     6961 cctgaagtta atggatcaat caagagagat gtgggctgta atgaatcgtc ttattgaatt
     7021 aacaggttgg atcgttcttg tcgtttcagt cattcttctt ggcgtggcga gtcacattga
     7081 caactatcag ccacctgaac agagtgcttc ggtacaacac aagtaagctc tgcacttgtg
     7141 gagcgacatg ctgcccgtcc gggtgcatgt tttcacttgt cggatattaa accaggaatt
     7201 tattatcttg ttcgatgttg ttggtgattg tcagggatag taaagcgcgt tactcttttc
     7261 agagcagttt ttcatccctg aatatttgcc atcagcgcat tcttattatt gtaataacac
     7321 aagcgttcac ttttggtgaa attttatcat cactaataat acctacccct ttggtattaa
     7381 ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg caaaatcagt
     7441 tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac ggacgatcag
     7501 gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt ttctggcggg
     7561 cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca ataaagaaag
     7621 tagcgatcgg ttataaaagc cttataataa cgcccctaaa attagatttg cctgtttgcg
     7681 tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag gctggttgct
     7741 tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt tgttgctgta
     7801 taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag cactgacgac
     7861 acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt acacactgtg
     7921 gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata ttatctggct
     7981 gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg aagttggcat
     8041 agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc cctatttcaa
     8101 acgctcgtcg cgggtaaaag cgacgtttgt gaatccgtaa taaccttaca gttaacctgt
     8161 tgtcgcctgc tctggattaa cggataatag gcggcttttt tatttcaggc cgaaaaatga
     8221 ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta ctggtcaagt
     8281 ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg atgggcatgg
     8341 ggctggcaac aacgtttgtg atgacgctga cgtctatttg cgcctggctt atcgatacgt
     8401 ggattttgat cccacttaat ctgatttacc tacgcaccct ggcatttatt ctggtgattg
     8461 ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagcccggtg ctttaccgtc
     8521 tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc gtggcgttgc
     8581 tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt tccgccgctg
     8641 tcggtttctc gctggtgatg gtgctcttcg ccaccatccg cgaacgcctt gctgtggctg
     8701 atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt cttatgtctc
     8761 tggcctttat gggctttagt ggtttggtga agttgtaatg aatgctatct ggattgccgt
     8821 tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg cctcccgccg
     8881 ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac cgcagagcca
     8941 gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca gctgtaacgg
     9001 tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa ttgccgagtt
     9061 gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc ctgcgcggat
     9121 ggtggcggtt attgatgaaa ataactgtat tggctgcact aaatgtattc aggcttgtcc
     9181 ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg atctctgtac
     9241 gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc aaccggtcgc
     9301 agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta tcattcccgt
     9361 ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg gatttcaacg
     9421 gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacccccctg cgccaggtac
     9481 cactggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa ggtgagttgt
     9541 gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt cgcggcaaaa
     9601 tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc cactctacgg
     9661 ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt gaagactgct
     9721 ggatcccgcg cgacggctgg gtcgattatc gctctcgcag tcgcgaagag ttaatcgagc
     9781 gcatacatca gtttggtgtt gccgggctgg gcggtgccgg attcccgaca ggcgttaaat
     9841 tgcagggtgg tggagataag attgaaacgt tgattatcaa cgctgctgag tgcgagccgt
     9901 acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta gagggtattc
     9961 gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa gataacaaac
    10021 cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt tctctgcggg
    10081 tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt ctgaccggga
    10141 agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa aacgtcggca
    10201 ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag cgtgttgtaa
    10261 ccctgactgg cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg ggaacgccag
    10321 tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg gtgattatgg
    10381 gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta aagattacca
    10441 actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa caaagctgca
    10501 tccggtgtag cgcctgtgct gacgcctgcc ctgtggatct tttgccgcaa cagttgtact
    10561 ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgcc gactgcattg
    10621 aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc cttggtgcaa tatttccgtc
    10681 aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca gaagccaaag
    10741 cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agcggctcgc cttgaacgac
    10801 ataagagcgc agccgttcaa cctgcagcca aagataaaga tgcgattgct gccgctctgg
    10861 cgcgggtgaa agagaaacag gcccaggcta cacagcctat tgtgattaaa gcgggcgaac
    10921 gcccggataa cagtgcaatt attgcagcac gggaagcccg taaagcgcaa gccagagcga
    10981 aacaggcaga actggagcaa actaacgacg cagcaaccgt tgctgaacca cgtaaaactg
    11041 ccgttgaagc agctatcgcc cgctccaaag cgcgcaagct ggaacagcaa caggctaatg
    11101 cggaaccaga agaacaggtc gatccgcgca aagccgccgt cgaagccgct attgcccgtg
    11161 ccaaagcgcg caagctggaa cagcaacagg ctaatgcgga accagaagaa caggtcgatc
    11221 cgcgcaaagc cgccgtcgaa gccgc
//
LOCUS       DAJTXY010000124        10799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTXY010000124 DAJTXY010000000
VERSION     DAJTXY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.124"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            77..1162
                     /locus_tag="OO566_003393"
     CDS             77..1162
                     /locus_tag="OO566_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6606557.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1233..1841
                     /locus_tag="OO566_003394"
     CDS             1233..1841
                     /locus_tag="OO566_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6606558.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1939..3330
                     /gene="selA"
                     /locus_tag="OO566_003395"
     CDS             1939..3330
                     /gene="selA"
                     /locus_tag="OO566_003395"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6606559.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVTHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3327..5171
                     /gene="selB"
                     /locus_tag="OO566_003396"
     CDS             3327..5171
                     /gene="selB"
                     /locus_tag="OO566_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6606560.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5361..6512
                     /gene="yiaY"
                     /locus_tag="OO566_003397"
     CDS             5361..6512
                     /gene="yiaY"
                     /locus_tag="OO566_003397"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6606561.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6677..8215
                     /gene="aldB"
                     /locus_tag="OO566_003398"
                     /pseudo
     CDS             6677..8215
                     /gene="aldB"
                     /locus_tag="OO566_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
     gene            complement(8434..8589)
                     /locus_tag="OO566_003399"
     CDS             complement(8434..8589)
                     /locus_tag="OO566_003399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606562.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            8760..9083
                     /locus_tag="OO566_003400"
     CDS             8760..9083
                     /locus_tag="OO566_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6606563.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9089..10225
                     /locus_tag="OO566_003401"
     CDS             9089..10225
                     /locus_tag="OO566_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6606564.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10222..>10799)
                     /locus_tag="OO566_003402"
     CDS             complement(10222..>10799)
                     /locus_tag="OO566_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6606565.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2576 a         2653 c         2973 g         2597 t
ORIGIN      
        1 accgaatttc ccggctggag catcaactcg ccgccgagaa aaagactgac tattccacgt
       61 tcccggagat ctaattatgg atctattgat tgttttaact tacgtggcgc tggcgacggc
      121 ggcgctggga ggcgtgtttc tggtgagtgg tttgattttg ttgatgaact acaaccaccc
      181 ttacactttt accgcgcaaa aggcagtgat agcgatccct atcacgccac aggtgacggg
      241 aattgttact gaagtcactg acaagaataa tcagcttatt caaaagggcg aggtgctttt
      301 taagctcgac ccggttcgtt accaggcgcg agttgacaga cttcaggctg acctgatgac
      361 ggcgacgcat aatataaaga cgctgcgtgc gcagctcact gaagcgcagg ccaacaccac
      421 ccaggtttca gcggagcgcg accgtctgtt taaaaattat caacgttacc tgaaaggcag
      481 ccaggcggcg gtgaatccgt tctcggaacg tgacatcgac gatgcgcggc aaaatttcct
      541 cgcgcaggat gcgttagtga aaggctcggt ggcggagcag gcgcagatcc agagtcagct
      601 cgacagtatg gtcaacggcg agcaatcgca gattgtgagc ttaagagcgc aacttactga
      661 agcaaaatat aatcttgagc agactgtcat tcgcgcacca agcaatggct acgtcactca
      721 ggtactgatc cgcccaggta catacgcagc tgccttgccg ctgcgtccgg tgatggtctt
      781 catccccgag caaaaacggc aaattgtcgc ccaatttcgg caaaactcgc tgttacgtct
      841 gaaacctggc gatgatgcgg aagtggtgtt taacgcgcta cctgggcagg tgttccacgg
      901 caaattgact agtattttac ctgtcgtgcc aggcggttct tatcaggcgc agggggtatt
      961 gcaatcatta acggtcgttc ccggcacgga cggtgtgctg ggaaccattg aactggaccc
     1021 taacgatgat atcgatgcct tacccgacgg catctacgcc caggtggcgg tttactccga
     1081 ccatttcagc catgtttcgg tgatgcggaa agtgctgcta agaatgacca gttggatgca
     1141 ttatctttat ttggatcatt gagacttata tcacctgtgt tttgcgtgag cctgagccat
     1201 actgatattt ttcctcgcac aaaggactcg gcatgaaact cgtcggtagc tacaccagcc
     1261 cgtttgtacg caaactttct attctgttgt tagaaaaggg cataactttc gaatttatta
     1321 atgaactgcc ctataacgcg gataacggcg tggcgcaatt taacccgtta gggaaagtgc
     1381 cggtgctggt gaccgaagag ggcgaatgct ggtttgattc gccgatcatc gctgaatata
     1441 ttgagttaat gaatgtcgct ccggcgatgt tgccgcgcga tccgctggag tcgttgcggg
     1501 tgcgcaaaat tgaggcgctg gcggatggca ttatggatgc cgggctggta tcggtgcgtg
     1561 aacaggcgcg tccggcggcg cagcagtctg aagatgaatt gttacgccag cgggagaaaa
     1621 tcaaccgcag tctggatgtg ctggaaggat atctggtcga tggcacactc aaaaccgata
     1681 cggtcaatct ggcgactatc gccattgcct gtgctgtcgg atatctcaat ttccgccgcg
     1741 ttgcgccagg ttggtgtgtc gaccgcccac acttagtcaa actggtcgaa aacctgttta
     1801 gccgcgaaag ttttgctcgc accgaaccgc caaaggcttg atgcgcgata tgtcctcctg
     1861 acccatctca cgttacaatc cgtggttatg ttaaacgccc ttctccgtgt gagagggcct
     1921 tgatcagcca ggtttcctat gacaaccgaa acgcgttccc tctatagtca acttccggct
     1981 attgatcgct tattgcgcga tagctccttc ctttctttgc gtgatactta tggtcacacc
     2041 cgcgtggtgg aattgttgcg tcagatgctc gacgaagcgc gagaagtgat tcgtggcagc
     2101 cagacgctgc ctgcgtggtg tgaaaactgg gcgcaagaag tcgatgcccg gttgacgaaa
     2161 gaggcgcaga gcgcgctgcg tccggtgatc aacctgacgg gaaccgtgct gcataccaac
     2221 cttgggcgag ctttacaggc ggaagccgcg gtggaagccg ttgcgcaggc tatgcgttcg
     2281 ccagtgaccc tcgagtacga tctggacgac gccggacgcg gacatcgcga tcgggcgctg
     2341 gcgcagctgc tgtgccgtat tacgggggcg gaagatgcct gtatcgtcaa taacaatgcg
     2401 gcggcggtgt tattgatgtt ggcggccact gccagcggga aagaggtggt ggtatctcgc
     2461 ggcgaactgg tggagattgg cggcgcgttt cgtattcccg atgttatgcg tcaggcaggc
     2521 tgcaccctac acgaagtagg gaccaccaac cgcacgcacg cgaatgatta tcgtcaggcg
     2581 gtgaatgaaa ataccgcact gttgatgaaa gtacatacca gtaactacag cattcagggg
     2641 ttcaccaaag cgatagacga agctgaactg gtggcgctcg gcaaagagct ggatgttcca
     2701 gtggtgactg atttaggcag tggctcgctg gtcgatctta gccagtacgg tttgccgaaa
     2761 gagccaatgc cgcaggagtt gattgcggcg ggcgtcagtc tggtgagctt ctccggcgac
     2821 aagttgttag gcgggccgca ggcagggatt atcgttggta aaaaagagat gatcgcccgc
     2881 ctgcaaagcc acccgctgaa gcgtgcatta cgcgcggata aaatgaccct cgcggcgctg
     2941 gaagccacgt tgcgtcttta tttacaccct gaagctctga gtgaaaaatt accgaccctg
     3001 cgcctgctta cccgcagcgc agaggtcatt caaatccagg cacaacgttt acaggctccc
     3061 cttgtcacac attacggcgc ggagtttgcg gtacaggtta tgccatgtct ttcgcagatt
     3121 ggcagtggtt cgctgccggt tgatcgcctg ccgagcgcgg cattaacgtt tacaccccat
     3181 gatggacgcg gtagccacct tgagtcatta gccgcccgct ggcgtgaatt gccagtgccg
     3241 gtgattggtc gtatttatga cggacgattg tggctggatt tacgctgcct tgaagatgag
     3301 caacggtttt tggagatgtt gttgaaatga ttattgcgac tgccggacac gttgaccacg
     3361 gcaaaacaac cttattgcag gcgattactg gcgtaaatgc tgaccgtctg ccggaagaaa
     3421 aaaagcgcgg catgaccatc gatctcggct atgcctactg gccgcagccg gatggtcgcg
     3481 tgcctggttt tatcgacgtt cccggtcatg aaaagtttct ttccaacatg ctggcgggcg
     3541 ttggtggtat cgatcacgcg ctgttggtgg tggcgtgcga tgacggcgtg atggcacaga
     3601 cccgtgagca tctggcgatt ttgcagctga ccggtaaccc gatgctgaca gtggcgctga
     3661 ccaaagccga tcgtgtggac gaagcgcgtg ttgatgaggt tgaacgccag gtaaaggagg
     3721 ttctgcggga atacggtttt gctgaggcaa aactgtttat caccgcagca accgaaggtc
     3781 ggggaattga tgccctgcgc gagcatctgc ttcagttgcc ggaacgcgag cacgccagcc
     3841 aacatagttt ccgcctcgcg attgaccgcg catttaccgt aaaaggtgcc gggctggtcg
     3901 tcaccggtac ggcgttaagc ggggaagtga aggtaggcga ttcactctgg ctgactggtg
     3961 taaataaacc gatgcgtgta cgtgcgctgc atgcgcaaaa ccagccaaca gaaaccgcca
     4021 atgccgggca gcgtatcgcg cttgacatcg cgggtgatgc ggaaaaagag cagattaacc
     4081 gtggcgactg gctgcttgcc gatgtgccgc cagagccgtt cacacgggtg attgtcgagc
     4141 ttcaaaccca tgcaccgctg acccagtggc agccgctgca tattcaccac gccgccagcc
     4201 acgtcacggg acgcgtttca ctgctggaag ataaccttgc tgaactggtc ttcgacaccc
     4261 cgttatggct ggcagataac gaccgcctgg tattgcgcga tatctctgcc cgcaacacgc
     4321 tggccggagc gcgcgtcgtg atgcttaacc cgccgcgtcg cggtaaacgt aaaccagagt
     4381 atctgcaatg gctggcttcc ctcgctaggg cacagaatga tagcgaagcg ttagccgttc
     4441 acctgcaacg cggcgcggtt aatcttcctg atttcgcctg ggcgcgccag ctcaacggcg
     4501 aagggatgcg cgaactgctg caacagcctg gctatattca ggctggttat agcttgttga
     4561 atgcgccggt tgccgctcgc tggcaacgga aaattctcga cacattagcg acttatcatg
     4621 agcaacatcg cgatgaacct gggcctgggc gcgaacgtct gcgacgtatg gcgttgccaa
     4681 tggaagatga agcgctagta ctgttgctga ttgaaaagat gcgcgaaagc ggcgacatcc
     4741 tcagtcatca cggttggctg catctgccgg atcacaaagc gggcttcagc gaagagcagc
     4801 aggccatctg gcaaaaagca gagccgctgt ttggtgacga gccgtggtgg gtgcgtgatc
     4861 tggcaaaaga gacgggtacc gacgagcagg caatgcgcct gacgctacgc caggcggcgc
     4921 agcaaggaat aattaccgcg atcgtcaaag atcgttatta ccgtaacgat cggattgtcg
     4981 aatttgccaa tatgatccgc gatctcgatc aggagtgtgg ttcaacctgc gcggcggatt
     5041 tccgcgatcg cttaggcgta ggccgaaagc tggcaattca gattctggaa tattttgacc
     5101 gcattggctt tacgcgtcgt cgtggaaatg atcatttatt gcgcgacgca ttattatttc
     5161 cggaaagata aggaaatgat taattaattt ttaaaataaa ttaatacaaa tttcttatga
     5221 ttttaaataa agcacattgt ttaatgaata caatgtgctt tttattagat taattttgat
     5281 aattaataat aatgatcatg ttttcgaatg aaaatcctca gtaagctgcc cacccttttt
     5341 tacactttca ggagtgtgtt atggcatctt caactttctt tattccttct gtgaatgtca
     5401 tcggcgctga ttcattgact gatgcaatga atatgatggc agattatgga tttacccgta
     5461 ccttaattgt cactgacaat atgttaacga aattaggtat ggcgggtgat gtgcaaaaag
     5521 cactggaaga acgcaatatt tttagcgtta tttatgatgg cacccaacct aaccccacca
     5581 cggaaaacgt cgccgcaggt ttgaaattac ttaaagaaaa taattgcgat agcgtgatct
     5641 ccttaggcgg tggttctcca cacgactgcg caaaaggtat tgcgctggtg gcagccaatg
     5701 gcggcgatat tcgcgattac gaaggcgttg accgctctgc aaaaccgcag ctgccgatga
     5761 tcgccatcaa taccacggcg ggtacggcct ctgaaatgac ccgtttctgc atcatcactg
     5821 acgaagcgcg tcataacaaa atggcgattg ttgataagca cgtgactccg ctgctttctg
     5881 tcaatgactc ctctctgatg attggtatgc cgaagtcact gaccgccgca acgggtatgg
     5941 atgccttaac gcacgctatc gaagcgtatg tttctattgc cgccacgccg atcactgacg
     6001 cttgtgcact gaaagccgtg accatgattg ccgaaaacct gccgttagcc gttgaagatg
     6061 gcagtaatgc gaaagcgcgt gaagcaatgg cttatgccca gttccttgcc ggtatggcgt
     6121 tcaataatgc ttctctgggt tatgttcatg cgatggcgca ccagctgggc ggtttctaca
     6181 acctgccaca cggtgtatgt aacgccgttc tgctgccgca tgttcaggta ttcaacagca
     6241 aagtcgccgc tgcacgtctg cgtgactgtg ccgctgcaat gggcgtgaac gtgacaggta
     6301 aaaacgacgc ggaaggtgct gaagcctgca ttaacgccat ccgtgaactg gcgaagaaag
     6361 tggatatccc ggcaggccta cgcgacctga acgtgaaaga agaagatttc gcggttctgg
     6421 cgactaatgc cctgaaagat gcctgtggct ttactaaccc gatccaggca actcacgaag
     6481 aaattgtggt gatttatcgc gccgcaatgt aatcatcatt ccaacaacgg ctggcaaatt
     6541 gtcagccgct ttttcaacta tctctgtaat ccttgcccgt aaattcgtga tagctgtcgt
     6601 aaagctgtta ccgactggcg aagatttcgc cagtcacgtc tacccttgtt atacctcaca
     6661 ccgcaaggag acgatcatga ccaataatcc cccttcagca cagattaagc ccggcgagta
     6721 tggtttcccc atcaagttaa aagcccgcta cgacaacttt attggcggtg aatgggtagc
     6781 ccctgccgat ggtgagtatt accagaacct gacgccggtg accgggcagc tgctgtgcga
     6841 agtggcgtct tcgggcaaac gagacatcga tctggcgctg gatgctgcgc acaaagtgaa
     6901 agataaatgg gcgcacacct cggtgcagga tcgcgcggcg attctgttta agattgccga
     6961 tcgaatggaa caaaacctcg agctgttagc gacagctgaa acctgggata acggcaaacc
     7021 cattcgcgaa accagtgctg cggatgtacc gctggcgatt gaccatttcc gctatttcgc
     7081 ctcgtgtatt cgggcgcagg aaggtgggat cagtgaagtt gatagcgaaa ccgtggccta
     7141 tcatttccat gaaccgttag gcgtggtggg gcagattatc ccgtggaact tcccgctgct
     7201 gatggcgagc tggaaaatgg ctcccgcgct ggcggcgggc aactgtgtgg tgctgaaacc
     7261 cgcacgtctt accccgcttt ctgtactgct gctaatggaa attgtcggtg atttactgcc
     7321 gccgggcgtg gtgaacgtgg tcaatggcgc aggtggggaa attggcgaat atctggcgac
     7381 ctcgaaacgc atcgccaaag tggcgtttac cggctcaacg gaagtgggcc aacaaattat
     7441 gcaatacgca acgcaaaaca ttattccggt gacgctggag ttgggcggta agtcgccaaa
     7501 tatcttcttt gctgatgtga tggatgaaga agatgccttt ttcgataaag cgctggaagg
     7561 ctttgcactg tttgccttta accagggcga agtttgcacc tgtccgagtc gtgccttagt
     7621 gcaggagtca atctacgaac gctttatgga acgcgccatc cgccgtgtcg aaagcattcg
     7681 cagcggtaac ccgctcgaca gcgtgacgca aatgggcgcg caggtttctc acgggcaact
     7741 ggaaaccatc ctcaactaca ttgatatcgg taaaaaagag ggcgctgacg tgctcacagg
     7801 cgggcggcgc aagctgctgg aaggtgaact gaaagacggc tactacctcg aaccgacgat
     7861 tctgtttggt cagaacaata tgcgggtgtt ctaggaggag atttttggcc cggtgctggc
     7921 ggtgaccacc ttcaaaacga tggaagaagc gctggagctg gcgaacgata cgcaatatgg
     7981 cttgggcgcg ggcgtctgga gccgcaacgg taatctggcc tataagatgg ggcgcggcat
     8041 acaggctggg cgcgtgtgga ccaactgtta tcacgcttac ccggcacatg ctgcgtttgg
     8101 tggctacaaa caatcaggta tcggtcgcga aacccacaag atgatgctgg agcattacca
     8161 gcaaaccaag tgcctgctgg tgagctactc ggataaaccg ttggggccgt tctgatataa
     8221 gaagctggtc gcattgggta ttcattgcct gatgcgacgc ttacgcgtct tatcatacct
     8281 acgggaacct gaccgtaggc cggataaggc gtacacgccg catccggcat aaacaaagcg
     8341 cacgttgtta caatttacgt tttaaaccta cgtgcgcaat aacccagact acgccaacag
     8401 aagtgcgttt ctggggcgtg ttttgtcagg agtttaatct cgcgtttcga tggtgagcgg
     8461 gcgaaccagg ccgtaaatca ccattggtga tccgaggcca atcagcaata aacggtagat
     8521 aattgaaggt ttaatacgct taatctgtac tgtggatgtt ttactggagt tattcattag
     8581 tttggccaat agttccacta tttcaatatg ataaacctcg tcctgttgaa aaaccagaca
     8641 gcacatttca gaaagtatat aaaaaacaat atgtcgttat ataaataagg aaattgcact
     8701 gcatcatggc atttcataat atgcgttaat tgttcttttt tatattcttt gagggaaata
     8761 tgttcctgga ctattttgca ctgggagtgc ttatttttgt atttctggtg atattctatg
     8821 ggattattat tttacatgat attccctacc tgatttctaa aaaacgtaat catcctcatg
     8881 ccgacgccat tcatgttgct ggttgggtga gtctttttac gctgcatgtt atctggccat
     8941 ttttgtggat ttgggccacg ctttaccgcc cggagcgggg atggggaatg caaatccatg
     9001 actcctccgt tgtgcaacta caacagcgta ttgccgggct ggagaaacag ctcgccgaca
     9061 tcaaatcctc ttctgccgag taatacttat ggatttactg attattttga cctatgtggc
     9121 ttttgcatgg gcaatgttta agatcttcaa aattcctgta aataaatgga ccatttccac
     9181 agcggccctg ggtggcatat ttattgtcag tggtttaatt ctgttaatga actataacca
     9241 tccgtatacc ttcaaggcgc aaaaagcggt tatttctatt cctgttgtcc cacaggtgac
     9301 aggcgtggtg atcgaagtga cggataagaa aaatacgctg attaaaaaag gtgaggtgct
     9361 atttcgactg gacccgacgc gttatcaggc gcgggtggat cggctgatgg cggatatcgt
     9421 taccgcagaa cataaacagc gggcgttggg cgcagagtta gatgagatgg cggcgaatac
     9481 tcagcaggca aaggccacgc gggataaatt cgctaaagag tatcagcgtt acgcacgcgg
     9541 cagccaggcg aaagtaaacc cgttttcaga acgcgatatt gatgtggcgc ggcaaaatta
     9601 tctggcgcag gaagcctccg taaagtcatc ggcggcggaa caaaaacaga tccagagcca
     9661 gctggatagc ctggtgttgg gtgaacattc tcaaatcgcc agcctgaaag cgcagctcgc
     9721 ggaagcaaaa tataaccttg agcagacgat agtgcgtgcg ccgagcgatg gttatgtgac
     9781 ccaggtgctg attcgtccgg gtacctatgc cgcgtcgctg ccgctacgtc cggtgatggt
     9841 gtttataccc gatcagaaac gacaaattgt ggcgcagttc cgtcagaact ccttgctgcg
     9901 actggcccct ggcgacgatg cggaagtggt gtttaatgct ctgccaggta aggtattcag
     9961 cggcaagctg gcagccatta gtccagctgt tcccggcgga gcttatcagt cgaccggcac
    10021 cttacagacg ttaaacacag cgccgggttc agatggcgtt atcgcgacca ttgaactgga
    10081 tgagcacact gatttgagcg ctttaccaga cggtatttac gcccaggtgg cggtctactc
    10141 tgatcatttc agccatgtct cggtgatgcg caaagtgcta ttacgcatga ccagctgggt
    10201 gcattacctt tatctcgatc attaatcttc gctttccgcc gcttgttcag gccgcagttg
    10261 ctgcgactga gcaagctggt ggcgaatttc acgcagcatt tcatccagca catactggac
    10321 ccgccagggc aggtattccc gtttacggaa gatagccacc agatccagcc accattcata
    10381 cgggtgctga aagcagggca ccagcgtgcc gtcttgcaga tcgctctgca cacttttgtc
    10441 tggcgcaaac acaatcccca ggtgatttcg tgccagctcc agcgccgatt gcgtgttatc
    10501 gcaaacgtaa tttcccttca cgcgataatc ccgcacctct ttactcccgg cgacgttgaa
    10561 gcgccagata ttcgcgtcgt cgatcatcat cgagtcgatc aaaatacagg agtgatgttc
    10621 gagttcgtca ggacggctaa taggatgttt ctcaagatag cgctggctgg cataagcggt
    10681 tacggcatat tgtgtaataa aactggcaac cagcgattca tcttttggtg gagcgtagct
    10741 gattaaaaca tcacaatcat caggaaattc gacgccttca taaaaggcat tacgctcaa
//
LOCUS       DAJTXY010000125        10758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTXY010000125 DAJTXY010000000
VERSION     DAJTXY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.125"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            18..1715
                     /gene="maeA"
                     /locus_tag="OO566_003403"
     CDS             18..1715
                     /gene="maeA"
                     /locus_tag="OO566_003403"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6606566.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1849..2859
                     /gene="adhP"
                     /locus_tag="OO566_003404"
     CDS             1849..2859
                     /gene="adhP"
                     /locus_tag="OO566_003404"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6606567.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3005..3289
                     /locus_tag="OO566_003405"
     CDS             3005..3289
                     /locus_tag="OO566_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6606568.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3517..4170)
                     /gene="fdnI"
                     /locus_tag="OO566_003406"
     CDS             complement(3517..4170)
                     /gene="fdnI"
                     /locus_tag="OO566_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6606569.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4163..5047)
                     /gene="fdnH"
                     /locus_tag="OO566_003407"
     CDS             complement(4163..5047)
                     /gene="fdnH"
                     /locus_tag="OO566_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6606570.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5060..8107)
                     /gene="fdnG"
                     /locus_tag="OO566_003408"
     CDS             complement(5060..8107)
                     /gene="fdnG"
                     /locus_tag="OO566_003408"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7520..7522),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6606571.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8339..9220
                     /gene="yddG"
                     /locus_tag="OO566_003409"
     CDS             8339..9220
                     /gene="yddG"
                     /locus_tag="OO566_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6606572.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9479..10579
                     /gene="ompC"
                     /locus_tag="OO566_003410"
     CDS             9479..10579
                     /gene="ompC"
                     /locus_tag="OO566_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6606573.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2640 a         2763 c         2659 g         2696 t
ORIGIN      
        1 aaaaaagagt gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt
       61 acgctggccc tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg
      121 aagaacgccg taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag
      181 aacaagcgga acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca
      241 tctacctgcg taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc
      301 atcttgatga gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt
      361 tttctgagat ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata
      421 tggacgatat tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg
      481 gtgaacgtat tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta
      541 aactgtcgct ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc
      601 tggatgtcgg aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta
      661 atccgcgtat cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga
      721 aacaacgctg gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc
      781 cgttacttaa ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg
      841 cggcggtaac agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg
      901 agaaaaaaat cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca
      961 tcgcccagac ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg
     1021 tcgatcgctt tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac
     1081 tggtgcagaa gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc
     1141 tggatgtggt gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc
     1201 tgtttacgga agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc
     1261 cgctgtctaa cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg
     1321 aaggtaacgc gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa
     1381 tctaccctat cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta
     1441 ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacacttg
     1501 ctcagtattc gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata
     1561 ttcagaaagt ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg
     1621 tggcggtcaa aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag
     1681 ccgaataccg cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac
     1741 cgggctattt ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc
     1801 tcatttgcct cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca
     1861 gttgttacga aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat
     1921 ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag
     1981 aatggcgatt ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg
     2041 gcagaagttg gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg
     2101 ttttacgaag gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt
     2161 tcagttaaaa atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc
     2221 accgattacg cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc
     2281 tgtgcggggg tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg
     2341 attgctatct acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc
     2401 tttaacgcca aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa
     2461 atgggtgcag atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag
     2521 aaaaccggtg gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg
     2581 gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct
     2641 atgagcctgg atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc
     2701 ggcacgcgcc aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg
     2761 aaagtcgccc tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc
     2821 aaaatccgtg gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc
     2881 atgttcgggt cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg
     2941 cgtaggaagg aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac
     3001 actaatgaaa atggccaatc atccccgccc gggggacatt attcaggaat cactggacga
     3061 acttaatgtc agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag
     3121 ccgattgctg accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt
     3181 gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc
     3241 agaaaaaacg gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc
     3301 ttataaagcg ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat
     3361 caggcctaca cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca
     3421 ttcacgacgc atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt
     3481 ttacagcgcc attttcagct taaagttcaa aagagattat atcccttctt cactctcttt
     3541 tttcgcttct gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg
     3601 acggctcacc ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata
     3661 catatggatc aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct
     3721 gtagcgaaca acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc
     3781 ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc
     3841 gttgtactta ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt
     3901 caacagccac ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat
     3961 aaacatcagt gcgacgaaaa tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg
     4021 cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga
     4081 cagcgccacc aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt
     4141 ggtgcgcaca atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct
     4201 tattcgggcc aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag
     4261 ccgctgccag cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat
     4321 ctttcggcag accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt
     4381 gcgtaccacc gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca
     4441 gtttcgccac gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag
     4501 ccccggtcgg acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc
     4561 agagcgtgca tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac
     4621 acccggcaat gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt
     4681 tagcgtactg aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac
     4741 agtgcataca gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc
     4801 taaagcgcat caccgtccag gacttggcgc tcagatcggc ggggttatcg taaacaccga
     4861 cgcagtgccc cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt
     4921 tacagccgat acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca
     4981 cctgagaagg cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca
     5041 tagccatctg ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg
     5101 gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac
     5161 gcgcgacacc ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt
     5221 ttacattcag cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct
     5281 tgctggagac agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc
     5341 tgatttccac aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg
     5401 tgtggaagtg ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac
     5461 ccatccgcag cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat
     5521 gcagcgggtt agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt
     5581 ccgccatttt gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc
     5641 cggttggcgt acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc
     5701 tgccgttcca ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg
     5761 aagcacggtt gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat
     5821 tccccagacc ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc
     5881 agctaccggt gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa
     5941 agctactcag caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt
     6001 ccaacgcata gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct
     6061 tgtagttcca gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca
     6121 actcgcgcag atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg
     6181 gcgcgtcctg acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat
     6241 cctcttcagc aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat
     6301 cgacatcgtt cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat
     6361 cgataaccac catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga
     6421 aggacgcaac cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca
     6481 tgttgaaata cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc
     6541 agttgttctc tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact
     6601 tcggatagtt gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt
     6661 ccagatacga ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc
     6721 tggtagagag cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt
     6781 tcacgccgcc accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac
     6841 ggatgttctg cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg
     6901 tgcgatccgg tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg
     6961 gcgtaccaca gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca
     7021 ggttccacac acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat
     7081 cgaactgata gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga
     7141 acaggccatc ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt
     7201 taacgtattc ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga
     7261 acgtaatgtc cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg
     7321 taaaacgggg atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc
     7381 agcggaaacc gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag
     7441 cgtttttgat atccacccag tggttggtca tagcacctcg accaaatgtt ggagcaagac
     7501 ttgctaccgt tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg
     7561 agcgggcaaa tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca
     7621 taccggtaga aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag
     7681 cgtcacggtc agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc
     7741 gctgccattt gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcactgt
     7801 tgacgtaatc cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat
     7861 ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc
     7921 tatacatcaa tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag
     7981 cgcgtaataa tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg
     8041 ccgctaccgt tgttccagcc atacctcccg cgcagatttt aaaaaattgt ctgcgactga
     8101 cgtccattgc tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat
     8161 tctgaatctt tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg
     8221 cgcaaattga cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta
     8281 aaaaaatgct ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat
     8341 gacacgacaa aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt
     8401 aggattgatt cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta
     8461 ttcattaagc gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc
     8521 gaaaggctat ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct
     8581 ttccttaggg tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct
     8641 gtggcccagc ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct
     8701 gattgtacca ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa
     8761 tgggttacat tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct
     8821 ggcgtttatt ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg
     8881 cggatttaac ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta
     8941 ttttctcacg ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc
     9001 tgcggcattt accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa
     9061 tgtcaccata atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc
     9121 cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa ggcgcgctaa tggtctgcgg
     9181 cggttccctg ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg
     9241 cccggcagtt aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa
     9301 ttcaccttat atctcatttc gacttcgatc acatatcgat aaataatatt atttcatcta
     9361 tttttctcat tgtttttaat caccattcta ttttaaaaac agtatgtcat ttataaatga
     9421 cgccgtttaa aattcgttta gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat
     9481 gaaattaaaa atagttgcgg tggttgtaac tggtttgtta gctgcgaacg tagcacacgc
     9541 tgccgaagtc tataacaagg atggtaataa actcgacctt tatggcaagg ttaccgctct
     9601 acgttatttt actgatgata agcgtgacga tggtgataaa acttatgccc gtctcggctt
     9661 taaaggagaa acgcaaatca atgatcaaat gattggtttt ggtcactggg aatatgattt
     9721 taaaggctat aacgatgaag ccaacggctc gcgcggcaac aagacccgtc tggcctatgc
     9781 tggtttaaaa attagtgaat ttggctctct ggactatggt cgtaactacg gtgtcggcta
     9841 cgacattggt tcatggaccg atatgttgcc agaatttggt ggcgatacct ggagtcagaa
     9901 agatgtcttc atgacatacc gtaccaccgg tctagcaacc tatcgcaact acgatttctt
     9961 tggcttaatt gaagggctga actttgccgc gcaatatcaa ggcaaaaatg aacgtactga
    10021 caacagtcat ctttatggtg ctgactacac gcgtgccaac ggtgacggtt tcggtatctc
    10081 ctcaacttat gtttatgatg gctttggtat cggtgcggta tataccaaat ccgatcggac
    10141 aaatgcgcag gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga atgcagaact
    10201 atgggccaca ggtataaagt atgatgccaa caacatctac tttgcagcta attacgctga
    10261 aacattaaac atgaccacct atggggatgg ttatatctct aacaaagcac aaagttttga
    10321 agtggtggcg caatatcaat tcgacttcgg cttgcgccca tcactcgctt acctgaaatc
    10381 gaaaggcaga gatctgggcc gctacgccga ccaggacatg attgagtata tcgacgttgg
    10441 tgcgacgtat ttcttcaaca aaaatatgtc gacctatgtt gattataaaa tcaacctgat
    10501 tgatgaaagc gactttaccc gtgccgtaga tattcgcacc gataacatcg tcgctacggg
    10561 cattacctat cagttctaac gtcctctcct gaccagccct tctctgggct ggtttttgat
    10621 ttaagtgctg ctgtatgact ccttaagtaa aaatgagcaa tttttgatat ttctggtcat
    10681 tcacctcaat taatattatt ccatatcctc cggggacatc ttactataat atttattgat
    10741 gagtgattat gaccaata
//
LOCUS       DAJTXY010000126        10754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTXY010000126 DAJTXY010000000
VERSION     DAJTXY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.126"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..447)
                     /locus_tag="OO566_003411"
     CDS             complement(<1..447)
                     /locus_tag="OO566_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6606574.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(502..1353)
                     /gene="manZ"
                     /locus_tag="OO566_003412"
     CDS             complement(502..1353)
                     /gene="manZ"
                     /locus_tag="OO566_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6606575.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1366..2166)
                     /gene="manY"
                     /locus_tag="OO566_003413"
     CDS             complement(1366..2166)
                     /gene="manY"
                     /locus_tag="OO566_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6606576.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2229..3200)
                     /gene="manX"
                     /locus_tag="OO566_003414"
     CDS             complement(2229..3200)
                     /gene="manX"
                     /locus_tag="OO566_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6606577.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFIPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3663..5213
                     /gene="yoaE"
                     /locus_tag="OO566_003415"
     CDS             3663..5213
                     /gene="yoaE"
                     /locus_tag="OO566_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6606578.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENCDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5217..6815)
                     /locus_tag="OO566_003416"
     CDS             complement(5217..6815)
                     /locus_tag="OO566_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6606579.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="OO566_003417"
     CDS             complement(6946..8310)
                     /gene="sdaA"
                     /locus_tag="OO566_003417"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6606580.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDNLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8494..9072)
                     /locus_tag="OO566_003418"
     CDS             complement(8494..9072)
                     /locus_tag="OO566_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6606581.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="OO566_003419"
     CDS             complement(9076..10437)
                     /gene="pabB"
                     /locus_tag="OO566_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6606582.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQKVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10511..10690
                     /locus_tag="OO566_003420"
     CDS             10511..10690
                     /locus_tag="OO566_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6606583.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
BASE COUNT         2680 a         2724 c         2752 g         2598 t
ORIGIN      
        1 tacgagaagc ttataaattt tttccagatc gtcgatattt cgaacgcgga ttaacagccg
       61 acgctgttct aattgcatca ccaacacgcc atcttccgac aagttcatcg ctttgattcg
      121 gctatattca atccagacat tggcgaagaa aaaacctttt tgtttaaaga tgatcttcgg
      181 aatgcggatc cagaatatat aaaaacccat cagagccagt gcacttaata accatgttgt
      241 tattagcgcg ccatgattcg tgacgttgtt atagataaga atgacaatca atccgacgaa
      301 gataacgcta tcgatgcgac cacggcggag caaaggaatt gccagcaggg tggggccgtt
      361 acggcggggc atgatgaact gatcgtagat cgcgaaggcc aggagtgcgg cgatgaaaag
      421 aatcagtacc aggtccgtga ttgtcattaa tcctccagat aaaaaaacgg ggccaaaagg
      481 ccccggtagt gtacaacagt cttacagtcc cagcaggccg caagcgtaac cagcgatacc
      541 gatgacgaag aagccaacga tgatccacag cgggttaact tttttgcgca gcagccacat
      601 acaagcaaat gtcagcagca gtggtacaag gcctggcatt aactggtcaa gaatagtttg
      661 cacggtggtg acacgggtct gtccatcctg cccggtgatg gtcgaaacca ccagcgggat
      721 gttgacatgt gtccacttgt taaccaatgc ccccatgaca aacaggccga ggatagacgc
      781 cccttccgtc agtttttgca ggaagccacc acccatatct ttaacgatat cgataccttt
      841 ggagtaacca taagctacgc cgtagtaacg ggttgccaga cgcaccaggt taaacaggat
      901 gaagaacagc agcggaccta acaggctgcc gctcatcgcg ataccggcac ccagtgctgc
      961 aaataccgga cgtacggttc cccagaagat cgggtcgcct acaccagcca gcggccccat
     1021 caaaccgact ttgataccgt tgatagcacc gtcatcgatc tctgcgccat tagcacgctg
     1081 ttcttccagc gccagggtta cgccgagaat cggtgcagcc acgaacggct gggtgttaaa
     1141 gaactccagg tgacggcgaa tagcttgttt acgagcttcg ttgttctcag ggtagaggcg
     1201 acgaattgcc ggtaccatag agaagcagaa acccagtgcc tgcatacgtt cgaagttcca
     1261 tgaaccctgg aagaggttag aacgcaggaa tacgccacga atatcacttt gagtgagttt
     1321 tttctcggtg gtagtttgag ttgtatcaac catttcgctc acctgttagt ccagttcgtt
     1381 atcgagatcg ttgttaccag ctgcctgagc aggcgcaccg gctacgcggt tgtatttcgg
     1441 gctaagttgg atgtagagca ctgccataac agtaccaatc acacccagag caaccaggtt
     1501 aaagttggtg aatgctgcgg ttacgaagcc gaggtagaag aacggcatca ggtagccagc
     1561 acgcatcatg ttgataacca tcgcataacc aaccaccacg atcatgcccc cggcgatatt
     1621 cagaccattg gtcaccactt ccggaatcgc attcagcatg ttctgtactt cgctggtacc
     1681 aacagacagc gcaacgatga cggccggaat agccacacgc attgcttgca ggaacagaga
     1741 agaaacgtgg atccaggaaa tcgctgtcag gttgccgtta tcagcagcct tatccgcagc
     1801 gtgctggaaa gcaacggtaa tagtacgaac gatgatggtc agtacctggc ccgcagcggc
     1861 cagagggatt gccagtgcga tacctgcacc aatgctctga tgacctgcga taaccagaat
     1921 ggtagaaatg atagaagcca gagcggcgtc aggcgcaact gcagcaccga tgttcatcca
     1981 gcccagcgcg atcatttcca gcgtaccacc gataataata ccggttttca tatccccaag
     2041 aacgataccc accagggtac acgcgattag cggacggtga aactgaaatt catcgaggat
     2101 tgatcccata cctgcgatac aggctacgat aaataccagc acaatttgaa gagtggtaat
     2161 ctccattgta cttctcctgt ttacgtaaga cttaagtgtg aaaactgaat gataatcaac
     2221 acaatacgtt acttatcgat tttgctgatc agatccatca ttttcagttt cggatcggtg
     2281 gaaaccttac ggacttccag ctcaataccg cgtgcattca gttttttgaa cgcctcgata
     2341 tctttttcat caaccgaaac cgcgttattc acctgggttt taccctgacg gaatgccata
     2401 ccaccgacgt taacagaggt gattttcacg ccgccttcaa cgagacgctc tacatctgtt
     2461 gggttggtaa ataacagcat tacgcgttcg ccagcatatt tcgggttgtt gtagacgcga
     2521 atcattttgg caacatcaac tacgtgtgct gttacgcccg gaggtgcaac ctgggtgagc
     2581 agtgtcttac gaacggtatc cgcagccact tcatcactaa caacaataat acgggagaca
     2641 ttggtttctt tggtccagcg ggtggcgacc tgaccgtgaa tcagacggtc gtcgatacgc
     2701 gcaaggccaa taaccatgta gtcgtttggc cccattggtt ttgccggagt tggagccgct
     2761 tttggtgctg cggcagcggg tgctggcgcg gctttttcaa ccggtttggc tttcagtgct
     2821 ttcacgcctt cacggcctgt ttctactgcc agcgcaacca gttcatcaaa gctcgggtca
     2881 tcatcacggg ccattaacgt ttccacgagc attggaatgt taacgcctgc aatgacttca
     2941 taatgctctt tgtcgacgac aatgcggctg gcagcattga acgggctgcc tccccatgta
     3001 tcaacgagaa acagcacgcc tttagtggtg tcgagttttg ccaactgagc gttgtatttt
     3061 tcaatcagcg tttcggcatt ttcacctgga atgaaatcga tccagccgac gttttcctgc
     3121 tcgcctaaca gcatttctgc cgttttaagc aactgctctg cagcccaacc atgtgtgcct
     3181 ataacaatag caatggtcac ttgctacctc ctttattatc gttaacacct caacgtgcca
     3241 gatgtatttt tgaatcgtcg tccacaatcg aatcgattca gataagggct acaaccaaaa
     3301 acccctgcgt ttcgcgattt attttagata tcgaaaaaat tattttatgt gatgaagatc
     3361 cgtaatttaa ctttcgatta tcagaaattt cgaaaggtaa aatatccttg tcacattcgt
     3421 ttgcaaagga aggtaaatct ttgccaaatc agaggcgtct ctgatatgtt taactcccgt
     3481 ttaacaacca tggagtatag ggcagtagcc cgcagtatgg atcgtcaccg acgtcatttc
     3541 agcatcaggc cttttaacgc ctgcctttct ggcactctat gccgcacctt tcgtttgcat
     3601 tttgtcgtta cgcctgcatt atttctggcg tcgaatagct attccttaag caggagcttg
     3661 tcatggaatt cttaatggac ccctcaattt gggcggggct actcacgctt gttgttctcg
     3721 aaattgtgct gggtatcgat aacctggtct tcatcgccat tcttgctgac aaactgccgc
     3781 caaaacaacg cgataaagcg cgtttgctgg ggttatcact ggcgctgatt atgcgtctgg
     3841 ggctgctgtc gctgatttca tggatggtca cgctgaccaa accgctattt accgtcatgg
     3901 atttctcctt ctccggacgc gatctgatta tgttgttcgg ggggatattc ttgctgttca
     3961 aagcaacaac cgaactgcat gaacggctgg aaaactgcga tcatgattcc ggccacggta
     4021 aaggctacgc cagtttctgg gtggtcgtca cacagattgt catccttgac gccgtcttct
     4081 cgttggatgc ggtaattact gcagtaggga tggttaacca tctgccggtg atgatggcgg
     4141 cggtagtgat tgcgatggcg gttatgttgc tggcatcgaa accgctgacg cgattcgtta
     4201 accagcaccc cacggtggtg gtgctctgtc tgagcttcct gttaatgatt ggtctgagtc
     4261 tggtggcaga aggtttcggt ttccacattc cgaaaggtta cctgtatgcc gcgattggct
     4321 tctcgatcat tatcgaagtg tttaaccaga ttgcgcgtcg caactttatt cgtcaccagt
     4381 cgactttgcc gctgcgagcg cgtactgccg atgccatcct gcgtttgatg ggcgggaaac
     4441 gtcaggccaa tgttcagcac gatgccgata acccgatgcc gatcccggaa ggtgcatttg
     4501 ccgaagaaga acggtacatg attaacggcg tactgacgct ggcgtcgcgt tctctgcgcg
     4561 ggatcatgac gccgcgcggt gaaataagct gggttgacgc taatctcggg gtcgatgaaa
     4621 tccgcgagca actgctctct tcaccgcaca gtctgttccc ggtatgtcgc ggtgaactgg
     4681 atgaaatcat cggtatcgta cgtgctaaag aactgctggt ggcgctggaa gagggcgttg
     4741 atgtggcggc gattgcttcg gcgtctccgg cgattatcgt cccggaaacc ctcgatccga
     4801 tcaacctgct gggcgtgctg cgtcgcgctc gcgggagctt tgttatcgtg accaacgagt
     4861 ttggtgtggt acaaggcttg gtcacgccgc tggatgtgct ggaagccatt gcgggtgaat
     4921 tcccggacgc tgacgaaacg ccggaaatca ttaccgatgg taacggctgg ctggtaaaag
     4981 gcggtacaga tttgcatgcc ttgcagcagg cgcttgatgt tgagcacctt gccgatgacg
     5041 atgatatcgc gacggtcgcg ggcctcgtga tctcggcaaa tggtcacatt ccccgtgtgg
     5101 gcgatgtgat tgatgtaggg ccactgcata tcaccatcct tgaagccaat gattatcgtg
     5161 ttgatctggt tcgcattgtt aaagagcaac cggcgcacga tgaagatgag taagcgttaa
     5221 cgtaacgaca taatgggcgt gatatgtcca ttatgccggg cgggcggcgg ttggctgccc
     5281 gccagccatt tgggaaaatc acgtagcggc atcggctgtg cgtacaaata cccttgcaaa
     5341 atatgcacgc catgacggcg caaatacttc gcctgttctt gcgtttccac gccctccgcc
     5401 accagttcaa tattcagtct ttgccccagc gcgatgatga tatcggtcac cgtcgagtta
     5461 accgcgtcag aacctatagc tgcggtaaat gacttatcaa ttttcagcac gtcaggacgt
     5521 aatgtttcaa gccaggaaaa cgagctgttg ccggtgccga agtcatcaat cgccagtttg
     5581 acgtttttac gatgcagctc gcgggcaatc cgataatcaa catccagtaa ggcatcgcgt
     5641 tcggtgattt cgaggatcag ttgctgaatc gggtgagcgc taaaccagta ctgattgaga
     5701 tcttttatca gcacaccacg gcgaaaatgg ctgggtgcga cgttaatgcc aacatgaaac
     5761 tgactactca tcgggaaaac atggcgctga cgaatggttt ctgccatcac atagcgggtc
     5821 agtggcacaa ttaaatgatg ttcttccgcg ataggaataa acacatccgg tgaaatccag
     5881 ccctgacgcg gattattcca gcgcagcaaa atctctacac caatacattg ctggctgcgc
     5941 gcattaagca aaggctgaca gaacaattcg aactcatgtt gcgccaggcc cagattaatt
     6001 tcgcgggaaa agctcatccg gtaagcggtc gccagccagg cgatatagcc caccagtaaa
     6061 cttagcagca ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc
     6121 cccggaccat ttacgttaat ggtaaaagga aagctttgcg aagaaacctg atagttttca
     6181 ttgtcttcag gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg
     6241 tccaccgtca ggctggcgct actgatttgc ggcagttgtg gctcaaggag cattgccgtc
     6301 agtaagtcga tgttaatcat ctctatgacc ccagcattgc tactgcctgc tgcaggcgtc
     6361 cattgaatca aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt
     6421 ggttgcggtg caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa
     6481 atgctggagc aataaagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt
     6541 tttgccgcct gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga
     6601 agcaagactt cgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg
     6661 tgattggcga attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg
     6721 ctcagggtcg aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgg
     6781 cgataggttt taatgatccg ctgtgctttt tgcatgcggg ccaccctgta agcgtatggc
     6841 gacaaacgcc tgacgcaaca gtggaagtgt agtggggaat attacagcag acgagaaagc
     6901 gggtataaat tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac
     6961 tttgattgcc agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt
     7021 ttcgtacatc gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag
     7081 agccatccgt gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg
     7141 cacctgaacc tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat
     7201 ttccgccgcc acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc
     7261 agccattgaa caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga
     7321 ggcgttcatt ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat
     7381 gtctgggctg accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc
     7441 gcaggcaccg ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac
     7501 tgccagcgca aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc
     7561 gctggaaact aacatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg
     7621 cagtacacct tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg
     7681 cgcgaaatac tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag
     7741 accagacagc gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa
     7801 cggatacggc acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat
     7861 aaaaccgccg ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc
     7921 gtggatttgc ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt
     7981 gtcgcgcggg aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttt
     8041 tacgtcgcga ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc
     8101 aagacccata ataatggcga tatcggtgtg gtggccttta cccgtcagcg acagtgaacc
     8161 ataaacgtcc acggcaacgc gagtaacgct atccagtaag cctttttcga ccagattatc
     8221 gacgaactgt ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc
     8281 caccttaaac atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt
     8341 ccagtaacga actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta
     8401 ttcacatgaa ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca
     8461 agtagtgttt tgtagatgta aagataagta tagtcagggg ttcacaccaa tttgcagcgc
     8521 cagctcacga attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag
     8581 ccatacccga tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg
     8641 taatgcctgg gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata
     8701 cggcagatcg ggcgggataa tgccgaccac tggggttacc tgatagccag ttacgctatc
     8761 gacgggcggc agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc
     8821 agcttcgcgc agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg
     8881 gaatgccact tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc
     8941 cggttgtggt cgacggacga tggggattaa cacggcagcc tgacgatgat ttagggtttc
     9001 ccggttaatt tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct
     9061 acggtattcc acgttttact tctccagttg gcgtaatatc ttattaactt tatcaaaagt
     9121 ttcctgatat tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta
     9181 tatttgtccg ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca
     9241 aaagctcaaa tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc
     9301 gataatttcc atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc
     9361 gcgcagcaga tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag
     9421 atgatgcacg gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc
     9481 tacggcaaca cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg
     9541 atctttcgct gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag
     9601 gcgtggtagc gtgcctttaa tcgggcgggt ctggatttca ctattatcac aaagaataaa
     9661 ccgctctggc gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg
     9721 cgcgcggttg gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc
     9781 atgaaaacgc tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac
     9841 ctggcgaaat ttttcgccgt actgctcgtg ggtcatattg gattgccagt cggaagtgag
     9901 cgtgaaatct tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac
     9961 atcattatga ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc
    10021 gtagataccc actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga
    10081 ctcaaaacgg cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa
    10141 atcttcgtta tgcgttgggc gaatgtctgc gcgatccagc accttctgga gcacctgtag
    10201 cgggtcatca gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttacctaa
    10261 agtggttaaa gtgcaaatcg gatcggcgac cacaatatca aagcggctat acggatgatc
    10321 ggcatagccg gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa
    10381 ttcagcggcg tcctgacgcc agggtaaagt aatcacagcg ggagataacg tcttcatcag
    10441 tcctgactct actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca
    10501 ggagttaaca atgtttgcag gtttaccttc actcacccat gaacagcagc aaaaagctgt
    10561 cgagcggatc caggaactga tggcacaagg gatgagtagc ggccaggcaa tcgcgctggt
    10621 ggcagaagag ctgcgtgcca accactccgg cgagctaatc gtggcgcgtt ttgaggatga
    10681 agacgagtaa cacactaatc cgtatcgatt tcgttataga agccgatgat catttgattg
    10741 tgagaaaaag gacg
//
LOCUS       DAJTXY010000127        10463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTXY010000127 DAJTXY010000000
VERSION     DAJTXY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.127"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(66..1070)
                     /gene="cra"
                     /locus_tag="OO566_003421"
     CDS             complement(66..1070)
                     /gene="cra"
                     /locus_tag="OO566_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6606584.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAEMVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1152..1238)
                     /locus_tag="OO566_003422"
     CDS             complement(1152..1238)
                     /locus_tag="OO566_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6606585.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO566_003423"
     CDS             complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO566_003423"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6606586.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO566_003424"
     CDS             complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO566_003424"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6606587.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3587..3631
                     /locus_tag="OO566_003425"
     CDS             3587..3631
                     /locus_tag="OO566_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606588.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO566_003426"
     CDS             complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO566_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6606589.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     EMEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGCDKGHQWMEEQLVSICKR"
     gene            5390..5476
                     /gene="leuL"
                     /locus_tag="OO566_003427"
     CDS             5390..5476
                     /gene="leuL"
                     /locus_tag="OO566_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6606590.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5569..7140
                     /gene="leuA"
                     /locus_tag="OO566_003428"
     CDS             5569..7140
                     /gene="leuA"
                     /locus_tag="OO566_003428"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6606591.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7140..8231
                     /gene="leuB"
                     /locus_tag="OO566_003429"
     CDS             7140..8231
                     /gene="leuB"
                     /locus_tag="OO566_003429"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6606592.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8234..9634
                     /gene="leuC"
                     /locus_tag="OO566_003430"
     CDS             8234..9634
                     /gene="leuC"
                     /locus_tag="OO566_003430"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6606593.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9645..10250
                     /gene="leuD"
                     /locus_tag="OO566_003431"
     CDS             9645..10250
                     /gene="leuD"
                     /locus_tag="OO566_003431"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6606594.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10299..>10463)
                     /locus_tag="OO566_003432"
     CDS             complement(10299..>10463)
                     /locus_tag="OO566_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6606595.1"
                     /translation="AATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVA
                     LFLTAKVKDV"
BASE COUNT         2736 a         2745 c         2562 g         2420 t
ORIGIN      
        1 gatgtccagt cccgtactct acgcgccaga gggaaattca cctggcgcgt atttttgttc
       61 gcggcttagc tacggctgag cacgccgcgg cgatagagat tacgtttaat gcgcgttaaa
      121 ccaggttttg gcttacgcgg ttcgtccagg cttgccagga caatctccag cacacgctct
      181 gcgacatcgc ggtgacgttg agccactgcc agcaccggac actgtaagaa gtcgagcagt
      241 tcgttatcgc caaaggtggc aattgccagg tcagaaggca gtttgccgtc gcgacgcagc
      301 gtgacatcca tcactccttg caacaacgca aacgacgttg tgaacagcgc ctgcggcatc
      361 ggatgcgttt ccagccattt ttcgaataac tgggcagccg cctcccgctc atagctgttg
      421 gcatacagga aatgcacttc gcgcggatca tctttccaag cagtacggaa accttgttca
      481 cgcaggaagc tgacagaaag ctccggcagc gcgccaagat aaagcaccat ctcggcggga
      541 aacttacgta actcttccgc cagcatttcg gcatcatcct gatcggcacc aaccacgctg
      601 gtgaaatgtt cacgatcgag ggcgcggtcc agcgcgacaa tcgggaacgg gtcgttagcc
      661 cagcgttgat aaaaaggatg ctcaggtggc aacgacgtcg aaacaataat ggcatcaacc
      721 tgacgctgta aaaggtgctc aatgcagcgc atttcgttgt ctggctgatc ttctgagcag
      781 gcaatcagca gttgataacc ccgttgccgc gcctggcgtt caagatagtt agcgatgcgg
      841 gtatagctgg tgttctccag atcggggatc acaagaccaa tagaacgtgt gcgtccagca
      901 cgaagcccag ctgccacggc gttcgggtgg taattgtgct cacgcaccac agccatgact
      961 ttttcaacgg ttttgtcgct cacacggtat tgcttcgctt tgccgttaat aacatagctt
     1021 gcagtggtcc gcgacactcc cgccagccga gcgatttcat ccagtttcac aattgcccct
     1081 tgcgtaaaat gtaaaaacca tagccattgt actggtatgg gttaaattca ttaacatcta
     1141 agcgcagaaa attaactgcg gcaactgctt ttattccagg ttcccactga tttcgcaaaa
     1201 aaagcccaac gtgacatgtt gggctgcaaa ttgcgcactg agatcattct caacgcatta
     1261 ttttatcgcc gcgcgaaagt ccgaccacac cagagcgagc aacttccaca attttcgcca
     1321 catcgcgaat cgatgctaaa aatgcatcaa gcttatcgct ggtgcctgct aattgaacgg
     1381 tataaagcga gggggtgaca tcgataattt gcccacggaa tatttccgta ttacgtttca
     1441 cttcgtcacg cccgtaaccg ctggcctgaa ttttcaccag catgatttcc cgctcaacat
     1501 gcgcgccctg ccccaactca ctcacgcgca agacatcgac cagcttgtgt aattgctttt
     1561 cgatctgctc aagtactttt tcatcgccca cggtctggat ggtcatacgc gataatgtcg
     1621 gatcgtcggt tggcgcaacg gtcaggcttt caatgttgta gcctcgctgg gaaaaaaggc
     1681 caatcacgcg ggataacgcg cctgattcat tttcgagtaa gactgataat atccggcgca
     1741 taatcaggtt ctctccgttt tgcttaacca catttcatcc attccgcccc cgcgaatctg
     1801 catcgggtag acgtgctcac tgccatcgac ggtaacatca acaaacacca ggcgattatt
     1861 gcgcacctgt tccagcgcct cgctaagttt gctttccagc tcatgcggat gagagatctg
     1921 gatcccgacg tgaccatagg cttccgccag acggacgaaa tcgggtagtg attgcatata
     1981 agattgtgaa tgacggccgg aatagatcat gtcctgccac tgtttcacca tccccagata
     2041 gcggttattg agattcacca ccagtacggg caactcgtat tgcaacgcgg tagacagttc
     2101 ctggatgttc atctgaatac tgccgtcgcc agtgacgcaa accacggttt cttctggcaa
     2161 cgccattttg acgcccagtg ccgcaggtaa accaaaaccc atcgtgccga gaccgccgga
     2221 attgatccag cgacgcggtt tgtcgaatgg ataataaagc gcagcaaaca tctggtgctg
     2281 cccgacatcg gacgtcacgt aagcgtcccc attagtcaac cgccaaagag tctcgatcac
     2341 cgcctgcggt ttaatctttt cactgtgagt gtcatatttc aggcactgaa gagcgcgcca
     2401 ctgttcaatc tgctgccacc agtcgcggat ctcatccagc ggttgatggg cggattcttg
     2461 cgacaggagt tcaagcattt gttcgaggac ctggcgagca tcccccacaa tcgggatatc
     2521 cgcagtcacg gttttagaaa tggaagtagg atcaatatcg atatgcagaa cagtggcatt
     2581 tgggcagtac tttgccagat tgttcgtcgt tcggtcatca aatcgtaccc cgacggcgaa
     2641 aatcacatcc gcgttatgca tcgtcatatt ggcttcgtag gtaccgtgca ttcccagcat
     2701 gcccagtgcc tgacgatgcg ttgccggaaa cgcccccagc cccatcaatg aggaaacaac
     2761 gggcagattc aacgcctcca ccgtttcttt caactgctga tggcagcccg ccatgattgc
     2821 cccaccgcct acgtagacaa ccggtttttt tgccgctacc agcgtttgca gagcacgctt
     2881 aatttgccct ttatgtccgg tagtagtggg attgtaagaa cgcatactga ccgactcagg
     2941 gcagacatag ggtaatttgt tcgccggatt aagaatatct ttcggtaaat caacgaccac
     3001 tggtcctggg cgaccacttg ccgccagcca gaaagccttt ttcagcacct gcggaatgtc
     3061 ttccgtttgc ttaaccagaa aactgtgttt aaccaccggt cgcgaaatcc ccaccatgtc
     3121 gcactcctga aaagcatcgt aacctatcaa cgaggtcgct acctgcccgg aaaggacaac
     3181 taatggaatg gaatccatat aagcggtggc gatgccggta atcgcattgg tcgcccctgg
     3241 acccgacgtt accagcacga cgccgacttc cccggtcgca cgcgccaggc catcggccat
     3301 atgcaccgcc gcctgctcat gacgaactaa tacatgatca ataccaccca cggtatgcaa
     3361 tgcatcgtaa atatcaagga ctgcgcctcc gggataaccg aatacttgtt taacgccctg
     3421 atcgataagc gatcggacga ccatctcggc tccagacaac atctccatgg cctgcctcac
     3481 tgtttgacgg aaaaaatgtg taaaacacat taaccgtccg atcttgctta agcaattggc
     3541 agccagagac ataaatgcag ggtaataaga ataaacagga ggaaaggtga aaagaaacga
     3601 taaaatcctc cattacgaag gattaaattg ataataaatc ggctgaatcc cacaacttac
     3661 agcaatttct ctattttttt aattaatcct aaaatagaga gtccacacat tcagaataaa
     3721 attctacatt caataaaaaa tgcagaataa accagacatt catgtctggc ctattctgca
     3781 ttcagttagc gtttgcaaat tgagactaat tgttcttcca tccactgatg gcctttatcg
     3841 cacccggcag cttcatgcca agagagataa caggttctgc tgttttgttt taacggcagc
     3901 ggtaatacct gtaattctaa ggattcagcg aactcttcag ccagccaacg cggcgcaata
     3961 gcgaccaaat gcgtttgcga caccacgcta agtacgctca tcattgccat gccctgatac
     4021 gcgatactgg cttgcttatc taccgtgtca taccaaggtt gactaaatga cgcgaaacga
     4081 tcgagcgaaa ccgccgcatg ttgttcgtta taaacatcat gtttcagtaa cgggccttta
     4141 attgttggat gatttttgct ggctaccagc accatttcat ctttaaataa tggcacgctg
     4201 gtaaattcag gacgatggaa gtcttcataa ctaatcacaa actccatttc ctgataacgc
     4261 agctgatgtt cagtgttctg atttaatgaa gacttgaaca taacatgtat atttggcgca
     4321 atctgctcaa tgtgattata aatctgcgag gtcagaatgc tgtctaacgg gctgcaaaca
     4381 caaagatgaa atacacgttc actgctcgcg ggttcaaaac ctgaaccagg caattcattt
     4441 tgtactagtt gcaatgcctg gcgaactgaa ccaaaaagtt gaaatgcgcg agcagtcggt
     4501 tgaataccac ggccataacg aacaaaaagc tcgtcattaa acatcacctt caggcgtgca
     4561 acagcgttac tgaccgcagg ttgcgacata cccagaacat gagcggcacg ggtaatgttt
     4621 tgctcctgca tcacggcatc gaaaacggtt aataagttga gatcgaccat gcgtagctgt
     4681 ggcttgctta actccgccgt ctctgaatga tctgtttgta cctctggcat acttaactcc
     4741 actgtcacac ttaactccct ttcccttatt ggaatgcaga gaaataatcc tgaagaatat
     4801 gatttatcat gcatataaaa taagaaaaag caaaatgagt aaaattcgga aaacataaag
     4861 atacgggttc tgataaaaac agaatccatt gccgtggaat gagtcattta ccacagcata
     4921 ataatccata atgtttttgt tgcgaaaaca atctaatcat aactaccgcg aatactcaat
     4981 catctacaaa atggattaaa tgtgcttatt taataattat tttatatatt taatgcatta
     5041 attcttaaca ttaattgatc aataatattc accaaatcaa tatcaaaaaa aatcgcaaaa
     5101 catataattc aatacaaatc attaggatag gttttgcaac gcgtgcattt tgtccccttt
     5161 ttcctcgttg attagatgca aaaatttatg ctgaaatatg tcaaccgatg aaaagcgtcg
     5221 gtagttaagc agaaattaat atcgcttact ttaaccaccg tagcacaatt agctaatttt
     5281 acggatgcag aactcacgct ggcgggacgt ttttattgcg tcagggttga catccgtttt
     5341 tgtatccagt aactctaaaa gcatatcgca ttcatctgga gctgatttaa tgactcacat
     5401 cgttcgcttt atcggtctac tactactaaa cgcatcttct ttgcgcggta gacgagtgag
     5461 cggcatccag cattaagcca gcacgcagtc aaacaaaaaa cccgcgccat tgcgcgggtt
     5521 tttttatgcc cgaagcgagg cgctctaaaa gagacaagga cccaaaccat gagccagcaa
     5581 gtcattattt tcgataccac attgcgcgac ggtgaacagg cgttacaggc aagcttgagt
     5641 gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta tgggtgttga cgtgatggaa
     5701 gtcggtttcc ccgtctcttc gccgggcgat tttgaatcgg tgcaaaccat cgcccgccag
     5761 gttaaaaaca gccgcgtatg tgcgttagct cgctgcgtgg aaaaagatat cgacgtggcg
     5821 gccgaatccc tgaaagtcgc cgaagccttc cgtattcata cctttattgc tacctcccca
     5881 atgcacatcg ccaccaagct gcgcagcacc ctggacgagg tgatcgaacg cgctatctat
     5941 atggtgaaac gcgcccgtaa ttacaccgat gatgttgaat tttcttgcga agatgccggg
     6001 cgtacaccca ttgccgatct ggcgcgagtg gtcgaagcgg cgattaatgc cggtgccacc
     6061 accatcaaca ttccggacac cgtgggctac accatgccgt ttgagttcgc cggaatcatc
     6121 agcggcctgt atgaacgcgt acctaacatc gacaaagcca ttatttccgt acatacccac
     6181 gacgatttag gcctggcggt cggcaactca ctggcggcgg tacatgccgg tgcacgccag
     6241 gtggaaggtg caatgaacgg gatcggcgag cgtgccggta actgttcgct ggaagaagtc
     6301 atcatggcga ttaaagttcg taaggatatt ctcaacgtcc acaccgccat taatcaccag
     6361 gagatatggc gcaccagcca gttagttagc cagatttgta atatgccgat cccggcaaac
     6421 aaagccattg ttggcagcgg cgcattcgct cactcctccg gtatccacca ggatggcgtg
     6481 ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat ctattggtct gaaccaaatc
     6541 cagctgaatc tgacctctcg ttcggggcgt gcggcggtga aacatcgcat ggatgagatg
     6601 gggtataaag aaagtgaata taatttagac aatttgtacg atgctttcct gaagctggcg
     6661 gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc tggccttcat cggtaagcag
     6721 caagaagagc cggagcattt ccgtctggat tacttcagcg tgcagtctgg ctctaacgat
     6781 atcgccaccg ccgccgtcaa actggcctgt ggcgaagaag tcaaagcaga agccgccaac
     6841 ggtaacggtc cggtcgatgc cgtctatcag gcaattaacc gcatcactga ctataacgtc
     6901 gaactggtga aatacagcct gaccgccaaa ggtcacggta aagatgcgct gggtcaggtg
     6961 gatattgtcg ccaactacaa cggtcgccgc ttccacggcg tcggcctggc caccgatatt
     7021 gtcgagtcct ccgccaaagc catggtgcac gtactgaaca atatctggcg tgccgcagaa
     7081 gtcgaaaaag agttgcaacg caaagctcaa cacaacgaaa acaacaagga aaccgtgtga
     7141 tgtcgaagaa ttaccatatt gccgtattgc cgggagacgg tattggtccg gaagtgatga
     7201 cccaggcgct gaaagtgctg aatgccgtgc gcaaccgctt tgcgatgcgc atcaccacca
     7261 gccattacga tgtaggcggc gcagccattg ataaccacgg gcaaccactg ccgcctgcga
     7321 cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg ctcggtaggc ggcccgaagt
     7381 gggaacattt accaccagac cagcaaccag aacgcggcgc gctgctgccg ctgcgtaagc
     7441 acttcaaatt attcagcaac ctgcgcccgg caaaactgta tcaggggctg gaagcattct
     7501 gtccgctgcg tgcagacatt gccgctaacg gcttcgacat cctgtgcgtg cgcgaactga
     7561 ccggcggcat ctatttcggt cagccaaaag gccgcgaagg tagcggacaa tatgaaaaag
     7621 cgtttgatac cgaggtgtat caccgttttg agatcgagcg tattgcccgc atcgcgtttg
     7681 aatctgctcg taaacgtcgc cacaaagtca cctcaatcga caaagctaac gtgctgcaat
     7741 cctctatttt atggcgtgaa atcgtcaacg agatcgccac ggaatacccg gatgtcgaac
     7801 tggcgcatat gtacatcgac aacgccacca tgcagctgat taaagatcca tcacagtttg
     7861 acgttctgct gtgctccaac ctgtttggcg acattctgtc tgacgagtgc gcaatgatca
     7921 ctggctcgat gggcatgttg ccttccgcca gcctgaacga gcaaggtttt ggactgtatg
     7981 aaccggcggg cggctcggca ccagatatcg caggcaaaaa catcgccaac ccgattgcac
     8041 aaatcctgtc gctggcactg ctgctgcgtt acagcctgga tgccgatgat gcggcttccg
     8101 ccattgaacg cgccattaac cgtgcattag aagaaggcat tcgcaccggg gatttagccc
     8161 gtggcgctgc cgccgttagt accgatgaaa tgggcgatat cattgcccgc tatgtggcag
     8221 aaggggtgta atcatggcta aaacgttata cgaaaaatta ttcgacgctc acgtagtgta
     8281 cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc cacctggtgc atgaagtgac
     8341 ctcaccgcag gcgttcgatg gtctgcgcgc ccacggtcgc ccggtacgtc agccgggcaa
     8401 aaccttcgcc actatggatc acaacgtctc tacccagacc aaagacatta atgcctgtgg
     8461 tgaaatggcg cgcatccaga tgcaggagct gattaaaaac tgcaaagaat ttggcgtcga
     8521 gctgtatgac ctgaatcacc cgtatcaggg gatcgtccac gtaatggggc cggaacaggg
     8581 cgtcaccttg ccggggatga ccattgtctg cggcgactcg cataccgcca cccacggcgc
     8641 gtttggcgca ctggcctttg gtatcggcac ttccgaagtt gaacacgtac tggcaacaca
     8701 aaccctgaaa cagggccgtg cgaagaccat gaaaattgaa gtccagggca aagccgcgcc
     8761 gggcattacc gcaaaagata tcgtgctggc aattatcggt aaaactggca gcgcaggcgg
     8821 caccgggcat gtggtggagt tttgcggcga agcaattcgt gatttaagca tggaaggtcg
     8881 tatgaccctg tgcaatatgg cgatcgaaat gggcgcaaaa gccggtctgg ttgctcctga
     8941 cgaaaccacc tttaactatg tcaaaggccg tctgcatgcg ccgaaaggca aagatttcga
     9001 cgacgccgtt gcctactgga aaaccctgca aaccgacgaa ggcgcaactt tcgataccgt
     9061 tgtcactctg caagcagaag aaatttcacc gcaggtcacc tggggcacca atcccggcca
     9121 ggtgatttcc gtgaacgaca atattcccga tccggcttcg tttgccgatc cggttgaacg
     9181 tgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa ccgggtattc cgctgaccga
     9241 agtggctatc gacaaagtgt ttatcggttc ctgtaccaac tcgcgcattg aagatttacg
     9301 cgcggcagcg gagatcgcca aagggcgaaa agtcgcgcca ggcgtgcagg cactggtggt
     9361 tcccggctct ggcccggtaa aagcccaggc ggaagcggaa ggtctggata aaatatttat
     9421 tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg tgtctggcga tgaacaacga
     9481 ccgtctgaat ccgggcgaac gttgtgcatc caccagcaac cgtaactttg aaggccgcca
     9541 ggggcgcggc gggcgcacgc atctggtcag cccggcaatg gctgccgctg ctgctgtgac
     9601 cggacatttc gccgacattc gcaacattaa ataaggagca caccatggca gagaaattta
     9661 tcaaacacac aggcctggtg gttccgctgg atgccgccaa tgtcgatacc gatgcaatca
     9721 tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt tggcgcgcat ctgtttaacg
     9781 actggcgttt tctcgatgaa aaaggccaac agccaaaccc ggacttcgtg ctgaacttcc
     9841 cgcagtatca gggcgcttcc attttgctgg cacgagaaaa cttcggctgt ggctcctcgc
     9901 gtgagcacgc gccctgggca ttgaccgact acggttttaa agtggtgatt gcgccgagtt
     9961 ttgctgacat cttctacggc aatagcttta acaaccagct gctgccggtg aaattaagcg
    10021 atgcagaagt ggacgaactg tttgcgctgg tgaaagccaa tccggggatc catttcgacg
    10081 tggatctgga agcgcaagag gtgaaagcgg gagagaaaac ctatcgcttt accatcgatg
    10141 ccttccgccg ccactgcatg atgaatggtc tggacagcat cggcctgacc ctgcagcacg
    10201 acgacgcgat tgcgtcttat gaagcaaaac aacctgcgtt tatgaattag accccttgcc
    10261 cggtcaaatg accgggcttt ccgctatcgt ccacgtcatc aaacgtcttt aacctttgcg
    10321 gttaaaaata atgcgacaac agaaataatc gcaattaccc agtagacagc aaagtgcccc
    10381 caactttgtg caattgctcc ctgaataaca ccagccagaa ttaccccggt agagatagtg
    10441 ttggtaaata aggtggtagc tgc
//
LOCUS       DAJTXY010000128        10249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTXY010000128 DAJTXY010000000
VERSION     DAJTXY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.128"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..1280
                     /locus_tag="OO566_003433"
                     /pseudo
     CDS             1..1280
                     /locus_tag="OO566_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
     gene            1332..2487
                     /locus_tag="OO566_003434"
                     /pseudo
     CDS             1332..2487
                     /locus_tag="OO566_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(2453..2575)
                     /locus_tag="OO566_003435"
     CDS             complement(2453..2575)
                     /locus_tag="OO566_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606596.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2778..4463
                     /locus_tag="OO566_003436"
                     /pseudo
     CDS             2778..4463
                     /locus_tag="OO566_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4501..6873
                     /locus_tag="OO566_003437"
     CDS             4501..6873
                     /locus_tag="OO566_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6606597.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNTPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            6930..9713
                     /locus_tag="OO566_003438"
     CDS             6930..9713
                     /locus_tag="OO566_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6606598.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETFESMG
                     LRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     SEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFLLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            10075..>10249
                     /locus_tag="OO566_003439"
     CDS             10075..>10249
                     /locus_tag="OO566_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6606599.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQ"
BASE COUNT         3025 a         2255 c         2214 g         2755 t
ORIGIN      
        1 ccgtgccgca ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga
       61 tgctcaggat ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg
      121 ttattacact gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc
      181 ggaagataaa caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg
      241 gcaacctcaa aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata
      301 ttacaactcc ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag
      361 cgatcagtta accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc
      421 ttttatgctt tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga
      481 tcaatatcag aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt
      541 ttattctgtt gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta
      601 tgacaataca attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc
      661 gctgaacggg gcgcaaaaag gctataagag tcagacctat cctggcggta ctcaccaccc
      721 caatgtttat gtggtggaaa ggaaaacttc aacccggtaa ttatgacaag ctgatttccg
      781 caatggattt ctacccaaca gctcttgatg cagccgatat cagcattcca aaagacctta
      841 agctggatgg tgtttccttg ctgccctggt tgcaagataa gaaacaaggc gagccacata
      901 aaaatctgac ctggataacc tcttattctc actggtttga tgaggaaaat attccattct
      961 gggataatta ccacaaattt gttcgccatc agtcagacga ttacccgcat aaccccaaca
     1021 ctgaggactt aagccaattc tcttatacgg tgagaaataa cgattattcg cttgtctata
     1081 cagtagaaaa caatcagtta ggtctctaca aactgacgga tctacagcaa aaagataacc
     1141 ttgccgccgc caatccgcag gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg
     1201 acagcagcca gccaccgctt agcgaggtaa atcaggagaa gtttaacaat atcaagaaag
     1261 cactaagcga agcgaaataa ctaaaccttc atgcggcgga tttttccgcc gccttattga
     1321 gcgagatagc aatgcacgtt acagccaagc cctccagttt tcaatgtaat ctcaaatgtg
     1381 attactgttt ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg atggatgaca
     1441 gcactctgaa agagttcatc aaacaatata tcgaagcgtc tggcaatcag gtctatttta
     1501 cctggcaagg cggtgaaccc actctggctg gcctggattt tttccgtaaa gttattcact
     1561 atcaacaacg ctatgcaggc caaaaacgta tttttaatgc attacaaacg aatggcattt
     1621 tattgaataa tgaatggtgt gcctttctca aagaacatga ttttctggtt ggtatctcga
     1681 tcgatggccc ccaggagtta catgaccgtt acagacgcag taattcaggt aacggtactt
     1741 ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata tcaagtagag tttaatacgt
     1801 taaccgtcat taataacgtt aatgtccatt accctcttga ggtttatcat tttttaacat
     1861 ctatcggcag taaacatatg caatttatcg aattgctaga aaccgggacg ccgaatattg
     1921 atttcagtgg tcatagtgag aacacattcc gtatcattga tttttctgtg cctcccacgg
     1981 cttatggcaa gtttatgtca accattttta tgcaatgggt taaaaacgat gtgggtgaaa
     2041 ttttcattcg tcagtttgaa agctttgtca gccgtttttt ggggaatggg catacaagtt
     2101 gtattttcca ggagtcctgc aaggataatc tggttgttga aagtaatgga gacatttacg
     2161 aatgcgacca ttttgtctat ccacagtaca aaattggaaa cattaataaa tctgaactca
     2221 aaacgatgaa cagtgtacaa ctgacagcgc aaaaaacgga tttcagcgaa atgtcagcaa
     2281 tgtgcatata aacctatctg caatggcggt tgtcctaagc atcgtattac taaagtaaac
     2341 aatgagactg tttcttattt ttgcgaaggt tataaaatcc ttttttcaac catggtacct
     2401 tatatgaacg ccatggttga gttagctaag aacagagtac cgctttacca cattatggat
     2461 gttgcaaaac aaatggagaa taattaataa cgctctcttt taactccatt cttagcctcc
     2521 ttcttatgac cttaacaagg cacccgacat aaattcctgt cgggtgtttc gtcatcatca
     2581 tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca gggcattagc aatacccttg
     2641 attatcaata gcataaccat aaccactgaa aattaaaaat ttctatatat ttcatattgt
     2701 taaatgatac ttaaataaat ttcatcagta aatcattgcc atagataatc attggatggc
     2761 acaatcaatt ttcgctaatg ataaccattc ccattacgtt ttgtatgttg atagcaaagt
     2821 atctctgtct gctgaagccc ttttggctgc gtaagaataa taaaacttca gtgttgttaa
     2881 tcattattat cctcgcaatg atccttggcg tggttaaaat tcaggtctgg ttaaacgact
     2941 ggaataatga ttttttcaat gcgctaagcc agaaagaaac cgataagctt tggcagcttg
     3001 ttctttggtt ccctgccctg ctggggatct ttgtattaat atccgtaaac aaaacatggt
     3061 taattaaact actgactatt cgctggcgcg aatggttaac agattactac cttaatcgct
     3121 ggtttgcaga taaaaactac tacttcacgc agatctacgg cgagcataaa aatacagata
     3181 accctgacca acgtatcgct gaagacattc tcttactgat caacaaaacg ctcagtcttt
     3241 catttggctt catccagtca cttagcatgc tgatcacctt taccgttatt ctttggcaaa
     3301 gcgcgggtac gctctcattc actgttggtg gaacagaatg gaatatccag gggtatatgg
     3361 tctataccgt cgtgctcatt gtgatcggtg gaactttatt tacccataag gtgggtaaac
     3421 gtattcgtcc gcttaatgtg gaaaaacaac gtagcgaagc gacatttcga actaatcttg
     3481 tgcagcataa taagcaggca gaactgattg cgctaagcaa tgctgagtct ctacaacgcc
     3541 aggagttgcg cgacaatttt cataccatta aagagaactg gcatcgttta atgaatcgtc
     3601 agcggtggct tgattactgg caaaatattt attcgcgctc gcttagcgtt cttccctact
     3661 ttctgttatt gccgcagttt attagcgggc agatcaatct gggcggactg atgaaatcgc
     3721 gccaggcatt tatgctagta tcgaacaatt taagctggtt tatttataaa tatgacgaac
     3781 ttgctgaact ggctgcggtt atcgatcgct tgtatgagtt ccatcaactc actgaatagc
     3841 gccctacgaa taagcctaaa aattgccaac atgcggtaca agtggctaat gcgagtattc
     3901 gtacgcctga taataagatc atattagaga acttgaactt tcatgtttcg ccaggcaaat
     3961 ggctattact gaaaggctac tctggtgcgg gaaaaaccac actgcttaaa acattatccc
     4021 actgctggcc gtgatttaaa ggtgatattt cttctcctgc tgacagttgg tatgtgtcac
     4081 aaacaccgtt aatcaaaagc ggcttactga aagagattat ttgtaaagca cttcccctgc
     4141 ccgtagacga taaatcgttg agcgaagtac tgcatcaggt tggtctgggg aaattggctg
     4201 cgcgtattca tgaccacgat cgctggggag atattctttc cagcggcgaa aaacaacgta
     4261 tcgccctggc acgattaatt ttacgacgtc cgaaatggat atttcttgac gaaactacct
     4321 ctcatcttga ggaacaagag gctatacgct tactgcgttt agtgcgtgaa aaactaccca
     4381 caagcggcgt cattatggtt acacatcaac ccggtgtctg gaacctggct gatgatattt
     4441 gtgacattag cacggtttta taacaacaat aaaagccctg ctgtacggag atataaataa
     4501 atgaagcgag ttcttattcc tggcgtcatt ttatgtggcg ctgatgtggc gcaggccgtc
     4561 gatgacaaaa acatgtacat gcattttttt gaagagatga cggtctatgc tcctgtccct
     4621 gtacccgtaa acggcaacac gcattacacc agtgaaagca tcgagcgttt accgaccggg
     4681 aatggcaata tcagcgatct gctgagaacc aaccctgcgg tacgcatgga ttcaacgcaa
     4741 agtacctcgt tgaaccaggg agatattcgc cctgagaaaa tctctattca cggtgcgtcg
     4801 ccctaccaga atgcctattt gattgacggt attagtgcaa ctaataacct gaacccagcg
     4861 aatgagtccg atgccagtag tgcaaccaat attagcggga tgtcacaggg gtattatctt
     4921 gatgtcagct tactggacaa tgtgacgctt tatgacagtt ttgtgccggt tgaatttggt
     4981 cgcttcaatg gcggggtaat tgatgcaaag atcaaacgct tcaacgctga tgatagcaag
     5041 gtgaaattgg gttatcgcac tacgcgtttg gactggttaa catcgcatat cgatgagaat
     5101 aacaagagcg catttaatca aggttcttca ggaagtacct atttctctcc agattttaaa
     5161 aagaactttt ataccttgtc gtttaatcag gagctggctg ataactttgg cgttaccgcc
     5221 ggtttatcgc gccgccagtc tgatatcacc cgcgcggatt atgtttcgaa tgacggcatt
     5281 gtcgccggtc gggcacagta taaaaacgtt atcgatactg cattgagcaa atttacctgg
     5341 tttgccagcg accgctttac ccacgattta accttaaaat ataccggctc cagccgtgat
     5401 tataatacca gcaccttccc gcaatctgat cgcgaaatgg gtaataaatc ctatggtctg
     5461 gcatgggata tggatacgca actcgcatgg gccaaactac gtaccaccgt tggttgggat
     5521 catattagtg attatacccg tcacgatcat gacatctggt acaccgaact ttcatgtaca
     5581 tatggtgata ttacagggcg ttgtacccgt ggcggattag gacacatttc ccaggctgta
     5641 gataattaca ccttcaaaac acgcctggac tggcaaaaat tcgccgtggg tgatgtttcg
     5701 catcaaccct acttcggcgc ggaatacatc tattccgatg catggactga acgccataac
     5761 cagtctgaat cctatgtgat taatgctgca ggaaagaaaa ctaaccatac catttaccat
     5821 aaaggtaaag gcagcctggg aattgacaac tacacgctgt atatggcgga tcacattagc
     5881 tggcggaatg tgtcgttaat gcccggtgtg cgttatgact atgacaacta tctgtcaaac
     5941 cacaatatct ccccgcgctt tatgacggaa tgggatattt ttgctgatca aacctcaatg
     6001 attaccgccg gttataaccg ttactatggc gggaatattc ttgatatggg attacgtgat
     6061 atccgcaata gctggacgga atcggtatca ggtaataaaa ccctgacgcg ttatcagaat
     6121 ttgaaaacgc cttataacga tgaactggca atgggattgc agcaaaaaat cgataagaac
     6181 gttattgcac gcgcaaacta tgtttaccgt gaagcgcatg atcaaatcag caaaagcagt
     6241 cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg caaaaccaaa
     6301 acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg ccaggtagat
     6361 attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc gttaaacaat
     6421 ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa tctggtctct
     6481 tacgatagcg ttccagtggc agattttaat accccattaa agatctcctt aaacatggat
     6541 ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca agaagcgcgt
     6601 aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata ttcagattac
     6661 aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg cttgtcctgg
     6721 acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct caatgtactg
     6781 gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc cagtggccgt
     6841 actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt aaccgggtaa
     6901 attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta ctgacgttag
     6961 tggcaactct gttgctcccc gggcggctga ttgccgccgc cttacagcag gatgaaaagt
     7021 taattaccgg gcaactggac aatggcttgc gatatatgat ttatccgcat gctcatccaa
     7081 aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa gaagacaatg
     7141 agcgcggcgt ggctcatttt gtagaacata tgatgtttaa cggcgcaaaa acatggccgg
     7201 gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtctc gatgttaatg
     7261 cctataccag ctatgacgaa acggtgtatc aggtgagttt gccgactacg cagaaacaaa
     7321 atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc tttgaaaaac
     7381 tcgaagtaga cgctgaacgt ggcgtaattt ctgaggaatg gcgtgcccat caggatgcga
     7441 aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt aatttagacc
     7501 gtgaacctat cggcctgatg gatactgtcg ccacggtcac accggcacaa ttgcgccaat
     7561 tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc gatatcgaca
     7621 gtaaagaagc gctggcgctg ataaaggata atttaagtaa gtttccggct aacaaagcag
     7681 ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat atcattaatg
     7741 ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg gtacaagtga
     7801 acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag ctgttcaatc
     7861 aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc ggcactgcgc
     7921 gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat gcacgagacg
     7981 ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt gatcagcatg
     8041 gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg ctgaaaaatg
     8101 cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg gcatccagct
     8161 cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg aaacgtctgt
     8221 ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga aagaaccagg
     8281 acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca ttgtctcctg
     8341 cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct tacatcttcc
     8401 caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt agcagcaaag
     8461 aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccaag gttattctgg
     8521 caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat aaaggcgatt
     8581 taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca gttagcggaa
     8641 gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa aactcggtaa
     8701 ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg cggactaata
     8761 accctgaacc tggtttcctg ttgattaacc agcgaatcac ccacagcacg attaacgaca
     8821 atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc gaccagcgtc
     8881 cagcggagaa attcgcccag ctgatgtatg agacgcgcta tgctgatgac cgcacgaaat
     8941 tactgcaaga aaatcagatt gcacagttta ctgccgcaga tgcgctggct gccgatcgcc
     9001 agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc gcagaagaca
     9061 aactcgtggc gttaattacg cgttacttag gctcaatcaa acactctgat tcgccattag
     9121 ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta aaagaacaaa
     9181 atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca cctgttaatc
     9241 tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat ctacgcgtta
     9301 atattcgtga acaggcatct ggggcataca gcgtttcttc tcgcctctcg gttgatcctc
     9361 aggctaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga catgatgaac
     9421 agttaacgtt agcgaatgaa gtgatggtta agcgtttgac taaggggatc agtgagcaag
     9481 aactgaacga ataccagcaa aacgttcagc gcagcctcga tatccaacag cgtagcgttc
     9541 aacaattagc gaacactatt ataaatagtc ttattcaata tgacgatcct gcagcatgga
     9601 ctgagcagga gcaattgttg aaacaaatga cgatagagaa tgttaacact gccgttaaac
     9661 aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa taataacagc
     9721 cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat gtaataattt
     9781 tataaatgcg ttcaaaataa taaccaagta ctaatagtga tattttaatg tctgattttt
     9841 acgtgataat tcaggaggca cagaaagcgc ataaaaataa caacataaaa caccttgcca
     9901 ccacccaaaa attctctatt gcattgtttt taatgaaaaa aatatcattc caacaatatc
     9961 tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca cttgcttact
    10021 ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt taaaatggat
    10081 aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg tgcgaagtct
    10141 atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga cgatgtcgca
    10201 tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcaga
//
LOCUS       DAJTXY010000129        10246 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTXY010000129 DAJTXY010000000
VERSION     DAJTXY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.129"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            80..883
                     /gene="hpaI"
                     /locus_tag="OO566_003440"
     CDS             80..883
                     /gene="hpaI"
                     /locus_tag="OO566_003440"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6606600.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            905..2260
                     /gene="hpaX"
                     /locus_tag="OO566_003441"
     CDS             905..2260
                     /gene="hpaX"
                     /locus_tag="OO566_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6606601.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2270..3160
                     /gene="hpaA"
                     /locus_tag="OO566_003442"
     CDS             2270..3160
                     /gene="hpaA"
                     /locus_tag="OO566_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6606602.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3187..4124
                     /locus_tag="OO566_003443"
                     /pseudo
     CDS             3187..4124
                     /locus_tag="OO566_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4351..5909
                     /gene="hpaB"
                     /locus_tag="OO566_003444"
                     /pseudo
     CDS             4351..5909
                     /gene="hpaB"
                     /locus_tag="OO566_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5927..6439
                     /locus_tag="OO566_003445"
     CDS             5927..6439
                     /locus_tag="OO566_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6606603.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6832..8982
                     /gene="btsT"
                     /locus_tag="OO566_003446"
     CDS             6832..8982
                     /gene="btsT"
                     /locus_tag="OO566_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6606604.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            9032..9235
                     /locus_tag="OO566_003447"
     CDS             9032..9235
                     /locus_tag="OO566_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6606605.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9246..10202
                     /gene="yjiA"
                     /locus_tag="OO566_003448"
     CDS             9246..10202
                     /gene="yjiA"
                     /locus_tag="OO566_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6606606.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2442 a         2669 c         2646 g         2489 t
ORIGIN      
        1 agccccgaaa aagagccagt cagttgctta actgactggc attactccat tagctgccgc
       61 tttgtttaag gagagaacga tggaaaacag ttttaaagcg gcgctgaaag caggccgtcc
      121 gcagattgga ttatggctgg ggctgagcag cagctacagc gcggagttac tggccggacc
      181 aggattcgac tggttattga tcgacggtga acacgcgccg aataacgtgc aaaccgtgct
      241 cacccaacta caggcgattg cgccctaccc cagccagcca gtggtacgcc catcgtggaa
      301 cgatccggtg caaatcaaac aactgctgga cgtaggcaca caaaccttac tggtgccgat
      361 ggtacaaaac gccgacgaag cccgtaaagc ggtacgcgcc acccgttatc cccccgccgg
      421 tattcgcggt gtgggcagtg cgctggctcg cgcctcgcgc tggaatcgca ttcctgatta
      481 cctgcaaaaa gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat
      541 gaagaactta ccgcagattc tggacgtgga aggggtcgac ggcgtgttta tcggcccggc
      601 ggatctgagc gccgatatgg gttatgccgg taatccgcag cacccggaag tacaggccgc
      661 cattgagcag gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc
      721 caatgagcaa ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt
      781 tgacaccacc ctgctcgccc gcgccgccga agcgctggca gcacggtttg gtaatagttc
      841 ttctatttcg atggcgaagc aaaacaacaa ctctgtctac taaacgtacg atgtggagcg
      901 catcatgacg acctctaccc tgcaatataa agataataaa actgttgaaa ttgaacatcg
      961 tgtcattaat aaattatttc gtcgcctaat catttttctt tttattctgt ttgttttttc
     1021 gtttcttgat cgcatcaata taggatttgc cggactgaca atgggcaagg atcttggcct
     1081 gacgtccaca atgtttggcc tcgccgcgac actcttttat gtcacgtacg tcatgtgcgg
     1141 gatccccagt aatatgatgt tagcgaaaat tggcgctcgt cgatggatcg ctgggatcat
     1201 ggtggtctgg ggaattgctt ccacctgcac aatgtttgca acaagccccg aaacactcta
     1261 tctactgcgt atgctggttg gtattgctga ggcgggtttt ttacctggta ttttggttta
     1321 tctgacctgg tggttcccgg catatcatcg ggccagagcc aatgccttat ttatgatagc
     1381 gatgccagtc actatgatgc tgggattgat cctttcaggc tacattctgg ccttggatgg
     1441 actgtggaat ctaaaaggct ggcaatggct ctttttgctg gaaggtctgc catcggtcat
     1501 tcttggtgtc gtcacctggt tttatctcaa cgacacacca gaccaggcga cctggcttga
     1561 tgaaaacgaa aaacaggctt taaaaacgat gattctccgt gaacaagaga gtgcatttgc
     1621 ccatgcatct gttccacgct caaccttacg tgaagtcctg accccagcca tcatgctata
     1681 tacacttgct tatttttgcc tgactaatac tctgagtgcg attaatatct ggacgccgca
     1741 aatcctacag agtttcaata ctggcagtag caacatcatg attggtttgc tggcggccat
     1801 cccccaattc tgcacaattt ttggcatgat ctggtggagt cgtcgttccg accgtcttaa
     1861 agaacgcaaa aagcatacga ttcttcccta cctgtttgcc gctgccggat ggttactcgc
     1921 ttctgcgaca gatcacagcc tcattcaact gagcggcatc attatggcct caatgggatc
     1981 atttaccgcg atgactatct tttggacaac acaggatcaa gttataagct tgcaatcgcg
     2041 tgccgttgcg atggctgtca tcaatgctgt cggcaatgta ggttcagcag tcagtccatt
     2101 acttatcggt attttgaaag atgcaaccgg cagttttagc tcaggtttgt ggtttgtagc
     2161 aggattgctg gtactgggcg cgttagtcat gacccgaatc cctatgggga gtcgggaaca
     2221 ctctctttca tctgaactta tcgcaccaaa accacaataa ggagtcatga tgtccagtga
     2281 agctatcttt gaacatggtt atgaaaatat tgatttggtc cgggaatatg atgcccgcta
     2341 cgctggtgaa gatgtgcatt atgaaacatt tacccggtta gcgaatttct ttggacgcga
     2401 tatgcgccct cactggcacg atagatattt tcaactccat tatctggtca ctggacgcat
     2461 tactttacaa ctggatgagc acttctattc tgtgcgcgca ccgctttttg tactaacacc
     2521 accatcggtg ccacatactt tctttactga tcctgatagc gatgggcatg ttctgaccgt
     2581 acgtcaggag ttaatttggc ccctggtaga aaagctctgg cctggtagtg gagatgccgc
     2641 aatgctacaa ggtatctgtc tgtcaccgga gggaatggaa catacactgg cggcattaaa
     2701 tcattattgg ccgataatat ccctggagtt tcaacaaaat aagccaggac gggaaattct
     2761 attgcaatca ctggcgcaaa gtatttttac aatcctactg cgtgaagcac caccaaatga
     2821 tatttcaaca tgcagcgtgc gcggcgagat gtgtttattc cagaagttta atcggctgat
     2881 tgacgaaaat tatcgccaac atttaatggt ccctgaatat gcaacaatgc tcggtatgag
     2941 tgaatcacgg cttaccgagc tgtgccgacg ttttgccaat caatcgccga agagattgat
     3001 tttcgaacga gtgagccggg aagccagacg gcttctgctc tatagcgagc aaagcatcaa
     3061 ccagatagcc cttgatcttg gctacaaaga tcccgcctat ttcgcccgtt ttttcaaccg
     3121 aatgatgggt tgctcaccaa cacaattccg tggtcgctga ccatttatca acctgagaga
     3181 ctcgacatga aaatatcttt acgctcacta tttgtggcgt gcctgattgc gccagtggca
     3241 ttcacctacg cctccgaacc gcaataaatg aaaattgtac tacttggaac gaaaggtggc
     3301 ccttctttat taaaagctga ccgtttaccg atggcaacgg cacttattat tggcaataaa
     3361 acctggttaa ttgatgctgg ttacggggcc agcttacaac tggtaaaaaa taatattcca
     3421 ctgcgccaga ttgataatat tttactcacc catttacaca gtgatcatat tctggactat
     3481 ccatcagtct taatgaatgc atgggctagc ggtctgcagg acaaaactat tcatgtattc
     3541 ggtccgccag gcacccgagc catgaccgct gccagttggc aggtcttcga tcgggacatc
     3601 acactgcgta tggaagaaga aggaaaaccc gatcctcgca aactcgttac agctcaagat
     3661 gttggtgaag gcgtagttta taaagatgat accgtgaccg tgactgcatt aaaggtccct
     3721 cattctcctt tccctgacgg tgaggctttt gcttatcgct tcgacacaca aggcaagcga
     3781 attgtcttct caggagatac ctcgtacttt cccccactgg caactttctc agaaggcgct
     3841 gatatacttg tgcatgaggc cgtacatgag ccctccgtcg ctaagctggc agacagtatt
     3901 ggcaatggta aaacactggc aaaagccatt gcttctcacc atacgactat tgaggatgta
     3961 ggcaaaattg ccaatgaagc aaaagtgaaa aaactcatcc ttagccacct ggtaccaatc
     4021 accgttccgg atgaaacatg gaagcaagaa gcacagaaaa ctattcagtc cctatcgtcg
     4081 ttggtcatga aaatatgacg attaccattc cattcgcccc ataaaaaatt ccgtccccgt
     4141 tagcccttgt tagcggggaa tattatgatt tgccttactg taagttgatc acgatcgcag
     4201 tttactattt aactccacct tgctgtcgaa aagtaccaac gttcgcctga accgtctctt
     4261 cccgacaacc gcgttcaacg cttcaattaa ataacaaaaa caacacacaa aattaacaat
     4321 cgtattccga ttaatactgt agaggtcgac atgaaaccag aagatttccg cgccagtacc
     4381 caacgtcctt tcaccgggga agagtatctg aaaagcctgc aggatggtcg cgagatctat
     4441 atctatggcg agcgagtgaa agacgtcacc actcatccgg catttcgtaa tgcggcagcg
     4501 tctgttgccc aactgtacga cgcgctgcac aaaccggaga tgcaggactc tctgtgttgg
     4561 aacaccgaca ccggcagcgg cggctatacc cataaattct tccgcgtggc gaaaagtgcc
     4621 gacgacctgc gccagcaacg cgacgccatc gctgaatggt cacgcctgag ctatggctgg
     4681 atgggccgta ccccagacta caaagccgct ttcggttgcg cactgggcgc gaatccgggc
     4741 ttttacggtc agttcgagca gaacgcccgt aactggtata cccgtattca ggaaactggc
     4801 ctctacttta accacgcgat tgttaaccca ccgatcgtca tttgccgacc gataaagtga
     4861 aagacgttta catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga
     4921 aagtggttgc caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac
     4981 aagtgatggg cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg
     5041 gcgtgaaatt aatctcccgc gcctcttatg agatggtcgc gggtgctacc ggctcgccat
     5101 acgactaccc gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg
     5161 tactgatccc atgggaaaac gtgctgatct accgcgattt tgatcgctgc cgtcgctgga
     5221 cgatggaagg cggtttcgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga
     5281 aactcgactt cattacggca ctgctgaaaa aatcactcga atgtaccggc accctggagt
     5341 tccgtggtgt gcaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat
     5401 tgagtgactc gatgtgttca gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc
     5461 atgccgcact gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca
     5521 ttatagaacg caacgttacc agtggcctga tctacctccc ttccagtgcc cgtgacctga
     5581 acaatccgca gatcgaccag tatctggcga agtatgtgcg cggttcgaac ggtatggatc
     5641 acgtccagcg catcaagatc ctcaaactga tgtgggacgc cattggcagc gaatttggtg
     5701 gtcgtcacga gctgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcaat
     5761 gtctgcgcca ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct
     5821 gcctgtcgga atacgaccag aacggctgga ctgtgccgca cctgcacaac aacgacgata
     5881 tcaacatgct ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga
     5941 acaacgcctg cgctttcatg acgcgatggt cagcctgtcg gcagcggtaa atattatcac
     6001 caccgagggc gacgccggac aatgcgggat tacggcaacg gctgtctgct cggtcacgga
     6061 tacaccaccg tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
     6121 gggcaacggc aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcgcgcca
     6181 cttcgcgggc atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
     6241 aggtccgctg gcgcagccgg tgctaaaagg ttcgctggcc agtcttgaag gagagatccg
     6301 cgatgtgcag gcgattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
     6361 cagtgcagaa ggtcacggac ttatttactt taaacgccgt ttccatccgg tgatgctgga
     6421 aatggaagct gcgatttaag ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat
     6481 tgtgatctca atcacatatc tacccgatga ataattcttg cccaaccgct caccgccatt
     6541 cctgaccgct tacatcccta aaataaccac tcagttattt accttacttt acgcgcacgt
     6601 atctctggca acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac
     6661 ctagaacggc ttcggcctat gattaacaaa ttactctatg gattttggtt tgcaggaagg
     6721 cagcaagtga gtgaatcccc gggagcttac aaaagtaagt gtcagaggtg aacgaacgca
     6781 gctgccgcac ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg
     6841 aaaaaactat tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc
     6901 gcggtagttg cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct
     6961 gtgtcggtgt atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg
     7021 aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt
     7081 ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg
     7141 ctggtgggac cggttctcgc cgcgcagatg ggctacctgc ctggcacgct gtggctgctg
     7201 gcgggggtag tgctggccgg tgcggtacag gactttatgg tgctgtttat ctcctctcgc
     7261 cgtaacggcg catctctcgg tgagatgatc aaagaagaga tggggccagt accggggact
     7321 atcgcgctgt ttggctgttt cttaatcatg atcatcatcc tcgccgttct ggcgctgatt
     7381 gtggttaaag ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg
     7441 attgcgctgt ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc
     7501 tctgttattg gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac
     7561 gatccgtact ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt
     7621 ggctatgcgt ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat
     7681 ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg
     7741 aacccggaac tgaaaatgcc tgcgatgacc cagtacattg acggtactgg cccgctgtgg
     7801 aaaggcgctc tattcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc
     7861 cacgcgctga tctcttccgg tacgacgccg aaactgctgg ctaacgaaac cgacgcgcgt
     7921 ttcattggct acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgca
     7981 gcgtccatca tcgaaccggg tctttacttc gcgatgaaca ccccgcctgc tggccttggc
     8041 atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag
     8101 ctgaaagacg ttaccgcaca cgcggcagcg accgtcagct cctggggctt cgtgatttcg
     8161 ccagagcaga tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca
     8221 ggtggtgcgc cgacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg
     8281 gctgacatgg gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc
     8341 gcgctggatg cgggtacccg ttctggccgc tttatgctgc aagacctgct gggtaacttc
     8401 atcccgttcc tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt
     8461 gtgggtctgt ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag
     8521 agcctgtggc cgctgttcgg tatctccaac cagatgctgg cagccgtagc gctggtactg
     8581 ggcaccgttg tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg
     8641 gctgtatggc tgcttatctg caccacctgg gcgctgggtc tgaaactgtt cagcaccaac
     8701 ccgcagatgg aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt
     8761 actgacctga cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc
     8821 aacgcaggcc tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc
     8881 aaaacctggc ttgcggtgcg taacagcgac aaacgtactg acaaagaaac accgtacgtt
     8941 ccaatcccgg aaggcggcgt gaagatctct tcgcaccact aaccgtgttt agccccgctt
     9001 cggcggggct ttgttcttat cagagtgaac tatgtttggt aacttaggac aggcaaaaaa
     9061 atatctcggc caggcggcga agatgttgat tggtattcca gactacgaca actacgtcga
     9121 gcatatgaag accaaccatc ccgacaagcc gtacatgagc tatgaagaat tcttccgcga
     9181 acgccagaac gcgcgctacg gcggcgatgg taaaggcggt atgcgctgtt gttaatggag
     9241 agagcatgaa cccgattgca gttaccctac tcaccggttt tttaggcgcg ggcaaaacta
     9301 ccctgctgcg ccatattctt aacgaacaac acggctacaa gattgccgtg attgaaaacg
     9361 aattcggcga agtctccgtt gatgatcaac tgattggcga ccgcgccacg cagatcaaaa
     9421 cgctgaccaa cggctgcatc tgctgttcgc gctccaacga gctggaggac gcactactcg
     9481 acctgctgga taatctcgac aagggcaata ttcagttcga tcgtctggtg attgaatgca
     9541 ccggcatggc cgatcccggc ccgattattc agaccttttt ctcccatgag attttatgcc
     9601 agcgctacct gctggacggc gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga
     9661 tgaaccagtt caccatcgcc cagtcccagg tgggctacgc cgaccgtatt ctgctgacta
     9721 aaaccgacgt cgcaggcgaa gcagagaaac tgcgtgaacg cctggcgcgc atcaacgccc
     9781 gcgcaccggt ctacaccgtc acccacggcg acatcgacct gggcctgctg ttcaacacca
     9841 acggttttat gctggaagag aacgtcgtca gcaccaaacc gcgtttccac tttatcgcgg
     9901 ataaacaaaa cgatatttcg tcaattgtgg tggaactgga ttacccggta gatatcagcg
     9961 aagtttcccg cgtgatggaa aacctgctgc tggaatcggc ggataaactg ctgcgttaca
    10021 aagggatgct gtggattgac ggcgaaccta accgcctgct gttccagggc gtccagcgcc
    10081 tctacagcgc cgactgggac aggccgtggg gcgatgaaaa accgcatagc acaatggtgt
    10141 ttatcgggat tcagttgccg gaggaagaga ttcgggcggc gtttgcggga ttgaggaagt
    10201 aagttcggga aagatcaact ttttgagagt tccaccgcaa cattat
//
LOCUS       DAJTXY010000130        10083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTXY010000130 DAJTXY010000000
VERSION     DAJTXY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.130"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            205..921
                     /locus_tag="OO566_003449"
     CDS             205..921
                     /locus_tag="OO566_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6606607.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLDAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1377..1452)
                     /locus_tag="OO566_003450"
     tRNA            complement(1377..1452)
                     /locus_tag="OO566_003450"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1417..1419),aa:Asn,seq:gtt)
     gene            complement(1553..3180)
                     /gene="yeeO"
                     /locus_tag="OO566_003451"
                     /pseudo
     CDS             complement(1553..3180)
                     /gene="yeeO"
                     /locus_tag="OO566_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3185..3260
                     /locus_tag="OO566_003452"
     tRNA            3185..3260
                     /locus_tag="OO566_003452"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3218..3220,aa:Asn,seq:gtt)
     gene            complement(3298..4248)
                     /gene="cbl"
                     /locus_tag="OO566_003453"
     CDS             complement(3298..4248)
                     /gene="cbl"
                     /locus_tag="OO566_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6606608.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4350..5267)
                     /gene="nac"
                     /locus_tag="OO566_003454"
     CDS             complement(4350..5267)
                     /gene="nac"
                     /locus_tag="OO566_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6606609.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            5594..5669
                     /locus_tag="OO566_003455"
     tRNA            5594..5669
                     /locus_tag="OO566_003455"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5627..5629,aa:Asn,seq:gtt)
     gene            complement(5725..6660)
                     /gene="ldtA"
                     /locus_tag="OO566_003456"
     CDS             complement(5725..6660)
                     /gene="ldtA"
                     /locus_tag="OO566_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6606610.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(6722..7801)
                     /gene="cobT"
                     /locus_tag="OO566_003457"
     CDS             complement(6722..7801)
                     /gene="cobT"
                     /locus_tag="OO566_003457"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6606611.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(7813..8545)
                     /gene="cobS"
                     /locus_tag="OO566_003458"
                     /pseudo
     CDS             complement(7813..8545)
                     /gene="cobS"
                     /locus_tag="OO566_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
     gene            complement(8542..9087)
                     /gene="cobU"
                     /locus_tag="OO566_003459"
     CDS             complement(8542..9087)
                     /gene="cobU"
                     /locus_tag="OO566_003459"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6606612.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(9714..9977)
                     /locus_tag="OO566_003460"
                     /pseudo
     CDS             complement(9714..9977)
                     /locus_tag="OO566_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2870 a         2561 c         2138 g         2514 t
ORIGIN      
        1 taaaagcaag tatgctttca aaacacaatt ataaaaaatc ccgccaacaa cattagtttt
       61 tataaaatta aatataagat tatggcttta gaatattttt atttctaata gacgagattt
      121 ttcctgttat gatataatat gccgaattaa cacatgttaa cgatttacca gtaatgtaaa
      181 taaattttcg aggagatcat tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa
      241 acggctaaag acggtgcaac gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct
      301 gctgccaaac aaggtgaacc cgatccagaa ttaaacacat ctttaaaatt cgttattgaa
      361 cgtgcaaagc aggcacaagt tccaaagcac gttattgata aagcaattga taaagccaaa
      421 ggcggcggag atgaaacgtt cgtacaggga cgttatgaag gctttggtcc taatggctca
      481 atgattattg ctgagacatt gacttcaaat gttaaccgta cgattgctaa cgttcgcaca
      541 attttcaata aaaaaggcgg caatatcgga gcggcaggtt ctgtcagcta tatgtttgac
      601 aatacgggtg tgattgtatt taaaggaaca gaccctgacc atatttttga aattttgctt
      661 gacgctgaag ttgatgttcg tgatgtaact gaagaagaag gtaatattgt tatttatact
      721 gaacctgctg accttcataa aggaatcgcg gcgctaaaag cagctggaat cactgagttc
      781 tcaacaacag aattagaaat gattgctcaa tctgaagttg agctttcccc agaagattta
      841 gaaatctttg aagggcttgt tgatgccctt gaagatgacg acgatgtaca aaaagtttat
      901 cataacgtcg caaatctcta attatatttt aaagaaatct gtctttatgg cagatttctt
      961 taatctcata taattcttat aaaaaatata atgtttaact cgtcatattg cttataaaaa
     1021 atccccgttc ccagagaaat aatatttatt aaaatggcag gtcttctttt tctgattacc
     1081 gaagacaaag tggcaattac gtatagtttc ccgataaaga cgcgacagct acatcccgca
     1141 taaggcattt ttctctttat ctttgtacgg tacttcatgg aacagaattt ttgaccttgt
     1201 gaatcgtgat gtctgttggg gaggggcaat ttgctcactg aagcgtgaga ctcgattaag
     1261 cgcacgaaac acagaaatca aaaaacccgg ccactttttt acaaagtggc cgggtaaaaa
     1321 taatttttat tttttaactg tcctgagtct catagagatg tctcaaaact aaaatttggc
     1381 tcctctgact ggactcgaac cagtgacata cggattaaca gtccgccgtt ctaccgactg
     1441 aactacagag gaatcgtgag aacgaggcga atattagcga tgcccaccca caatgtcaaa
     1501 gcctgttttt taaatttgaa atcgtttgct gaaataatct gcattttgtc gtttattccg
     1561 acacaactgg ctttttttca cacttttgcg gctcggatcg agggtatttc cacagccaac
     1621 gtccagtaac cattcgccag taaaacagca cggcccgcac agcccagtag gcaaacattc
     1681 ccatccagac accaaccaca ccccagccaa gcatgattcc ctgcacataa ccgactacaa
     1741 cccggcaacc ccacatgctc aacatagaaa cccacatggc gtaacgggca tcacgagcac
     1801 ccttaaatcc agcgggtagc acccatgagg cggaccaaat aggcataaat aaagcattta
     1861 gctaaatcag aatcacaacg acgtgtttaa cctgtggatc ctgggtgtaa aacgatgcca
     1921 taaccccggc aaagggagcc gttagccagg cgatggccgt taatccaaga gtggacagcc
     1981 agaacacatg ccgcaactga atctctgctt gcgctatctg ccctaccccc aaccttcggc
     2041 ctgtaatgat cgtagaagca gaaccgagcg cacttccggg taagttgata agagccgcaa
     2101 ttgagaacgc gataaaattt ccggcaataa cgctggtccc catcccggca acgaacattt
     2161 gggttaataa ccgaccactg gtaaataaca ctgattcgac actcgctgga ataccaatcc
     2221 ccatgacttc ccagataatg ctaaaattca gcggtttaaa atagctcttt aacgaaatcc
     2281 ttagcgcagg attaaaacca atcgccagca cccacaaaat tgcaactgcg ccaatataac
     2341 gagaaatggt taaacccaac ccagccccga ccaggagaaa aggccgtaaa tcaatatgcc
     2401 gctaataata atattaagaa tattcaggct accgttaatc agtagcggta ttttcgtatt
     2461 ccctgcacca cgaagtgccc cgctaccaat aagagtgatg gcagcggctg gataactgag
     2521 tacggtcagc tccagataag tcaacgccag tgctttaact tctgtcgtgg catcacccgc
     2581 gacgaaatca ataatttgtt cgccaaaatg atgaattagc gttgccaaca gtacggcaaa
     2641 caacgtcatg atcaccaatg actgccgcgt cgccaccctc gctcgtcgtc gatcccgctt
     2701 accgagacta aatgctacaa cgacagtagt accaagatcg atagctgcaa aaaaagccat
     2761 aatgaccata ttgaagctgt ccgccaaccc cacgccggcc atcgcatctt ttcccagcca
     2821 gctgaccaga aaagtgctca ggccccccat caacaggaca cagacgttct ccatgaagat
     2881 aggaacagca agcggggtta tctcgcgcca gaacaacact ttgtagctct tgcgtttaga
     2941 gtgccagcga gtgccgtgaa caacctggcg taaagcagag gagatattca aagccgacct
     3001 taattgcaga aagtgaaacc acatttcaaa tgatgaggga gaatcagcaa agctgcaaag
     3061 attttcgcca acaaattgtc tgcaaatgca acaaactgtt gatagaaacg gcaaacagtt
     3121 gggaaattta aaaatcgggt ttgacaaaat atttttcgcc gttaagatgt gcctcaacag
     3181 cgattcctct gtagttcagt cggtagaacg gcggactgtt aatccgtatg tcactggttc
     3241 gagtccagtc agaggagcca aatttctgaa aagcccgctt ttatagcggg ctttttgcta
     3301 tatctgataa tcaatttcct cttcactgtt ttccatcacc tggcgcttga tatcctcaac
     3361 tgacagtcct gcattacaaa gttccagaaa gcgccaaaca tagttacgct gaagttgtcc
     3421 tcgcttcagt cccaaccaga cagtattagc atcgaaaaga tgccgcgtat ccaggcggat
     3481 taaattctct tcctcttgtt cgccgctgga ttgttcggca actaatccga tcccaagccc
     3541 aagagcaaca taggttttaa tgacatcaga atcctgtgca cttaatacaa tatctgccgg
     3601 caaacctttg cgggtaaatg cgtcatcagt gcgtgagcgc cccgtaattc cctgtcggta
     3661 agtaattaac ggccacttcg ctattgattc cagcgtcaat ggtgtaattt gcgttaaggg
     3721 atgatcgagt ggaacaagca aactatggtg ccaacaaaac cacgggaagg cgacgagctg
     3781 cgggtcatta ctcaaacgct cgctggcgat accaatatca gcttcgccat tttgcaacaa
     3841 cgtcgcaatt tcctgtggcg aaccctggat tagctcgagc cgaacctccg ggaaaagttc
     3901 gcgaaaagct ttaatgacct ctggcaagct ataacgtgcc tgagtatgcg tcgttgcaat
     3961 agtgagaaca ccagacgtat cgttggtaaa caggtctgca agccgacgaa cattactggc
     4021 ttcattcaga atacgttctg caatgaccag taatgctttg cccggttcag tcatccccag
     4081 cagtcgctta cctcgtcgaa caaatatttc gatgccaagt tcatcctcca gttcccgaat
     4141 atgacggcta acgcctgact gtgaggtaaa aagcatattc gcaacctctg tcaggttgta
     4201 atcctgacgt gcagcctcgc ggattatctt tagttgttgg aaattcacgg taaactccgg
     4261 gcagttcaga tatcccgtta ttgttaaagt ctaatgcccg gcataacaaa taataaaaac
     4321 ccgcatctta ttccatcccg atataacgct tagctcacca attgccactg ccttttttcc
     4381 atcactggag aactaatcac tgacattaac aactctttca ctgcctgtgc ctgtggtgac
     4441 aagtttgctc tggcgggtaa atttaaggac aaagagagac tcattgaagg agtggtaatg
     4501 cgtgacatcc acccatttac tgcgccacat aacgaacgcg cagccgattc aggtaatact
     4561 gcaacgccca tgccgctggc aatcgctgcg gtaagcgtgg caatagactc aatttcacca
     4621 atgacttttg ccgtgagccg ccgtagggaa aaagcctcat caacacgaag tctaacagcg
     4681 ctgtaatcac tggggagaaa aaggttcatt tgcgcaatag cattaacatc aacgttttgc
     4741 cccgggcaat cttgagttcc taccaggaaa agatcttctt tcagcaaagc ctgactggat
     4801 accccagcca caggggaatg ctcataaatc accgccatat cgagttggtg atttatcagt
     4861 ttttcgttaa gcactgcgcc actattttca tgaagataga taacgacctc cgggaattca
     4921 gcgcgaaccg cctgtaataa gggcatggtg atggatgacg cagcggttcc tggcgcaaag
     4981 ccaatcgaga cttgccccga taatgactga ccaacgttat gcaccgccag ttgggcctgc
     5041 tcacactgac gtaaaatggc ccgtgcatgg gtatagagaa tttttccggc gtctgttggc
     5101 gtaacgcccc gttttgtgcg aatcaaaagc tgttgattta actcaccttc cagtatggca
     5161 acctgctggc tgagcgctgg ttgtgcgata tgcaatactt cagcagcctg ggtcaggcta
     5221 ccaatatcta caatttttac gaagtatttc aggcgtctga agttcatgtt gcctccggtt
     5281 tttaagaatc ggcccaagtg ccgccattac ttacaaccag tatgcaagat gcctgccagt
     5341 tctattttgg cgttgatgcg caagctaacc aactgtcaaa taagagatta tgatagattc
     5401 gtcattggcc ctttttatca gcagtcgcat tcacctgccc tataaaagga gggtatgcac
     5461 cacgatggtt cattacccaa taagattgaa agctcaccac tttgttgaaa ttggcagcaa
     5521 acaaacaaaa aaatgcattt caccctttga catcaccatg cactgccatt aatatgcgcc
     5581 ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     5641 cactggttcg agtccagtca gaggagccaa attcaaaaaa gcctgctttc gagcaggctt
     5701 tttactttct aattaccaac gctcttaaaa catctgtctt gaaccagaac taatttgcac
     5761 aggcattccc gatcgatgtt gcaacgcagc atttgcgcga tttacatcaa cttcttgccc
     5821 gttgataaac gcccgcaaag atggggttac cggcaatggc acttttcggt cagactcata
     5881 ttctgcacga ttgcgtgaca gcggctcatg aacttccagc cactttgaac catccggttc
     5941 cgttgtgtat ttcactggct gatcaataat ttgtacacgt gtgcctacag gaacattatc
     6001 aaacagatat ttgatatcgt cattacgcag acgaatacag ccctgactta cacggagccc
     6061 aataccaaaa ttggcattgg taccgtggat ggcatacaac ctgccaatat aaatcgcgta
     6121 cagccccatg gggttatcgg gccccgcagg aacaaatgcg ggcaaactct cccctcgttt
     6181 cgcatattcg cgccgggtgt tcggcgttgg cgtccaggtt ggtgcttctt gtttacgttc
     6241 aacggtagtc acccagttac gcggggtttc tcgcccagcc tggccgatac caataggaaa
     6301 gacttccact gtattactgt ctggcggata gtaataaaga cgcatttcag cgacgttaac
     6361 cacaatccct ttacgaacag tggcgggcaa aatcagttgc tgcggaatgg tgagttgtga
     6421 gccaggcttc ggtaaaaaaa catcagcgcc cggattcgct tccagcatat tacttaaccc
     6481 ttgcccgtat tgtgcggcaa aagtctccag cggctgagta ttgtgatcag gaacagttac
     6541 agtaagcgac tgccccacta aacggctacc ctctggaggt aatggataag ttaccgccag
     6601 gctattttgg ctggcaaaaa gcagagcaaa tgagcaaaga atatttacac gacgcatcat
     6661 gtccctttcc taagtcgcga atactatccg ataagtatag cttttatcag acttttcgtt
     6721 tttagctgtt caaatcagaa gtcgtattcc ccggtagaac aatattactg gcagcaagtt
     6781 cgcccatgtt gttgtatatc gcacaagcag cttcgatgat ggacatcgcc agagctgcac
     6841 cactcccctc acctaaacgc atatccatat tgagataagg ctccagcccc aaatgcgaga
     6901 gcgctatacg cgcgcctttt tctgccgaca agtgagaagg aatgagatac ggtttgattg
     6961 caggagacat ctggcaggct gcgagcgcag cagcataaga aagaaatcca tccagcaaca
     7021 cgggtaaacc gcaggaagca gcacctaaca tcactccagc tattccgacc aaatcaaatc
     7081 cacccacttt tgccaggaca tcaacaccat cctgaggatt tggttgattc aacgtaatcg
     7141 cccgacgcac aacatcaatt ttattagcca gtttatctgt cggcaggttt gcgccaatcc
     7201 caaccacttc ttcaggatcc cggccagtga ttgtgctgac tattgccgct gccggtgtcg
     7261 tgtttgccat ccccagttca cctacaccaa acagcgtgac accgtttttc gccagctccc
     7321 gcgtataaca tatgacgtcc aaaagcaact tttcagcctg acggcgactc attgccggag
     7381 ctgaagcaat attgccgcta cctcgtgcga cacgcatgtt gataagcccg gggataggct
     7441 cagcagtatc aataccaaca tcaattacgt ggacgttagc gcccgcttgt gctgccagca
     7501 cacacacgcc ggttgttcca cgggtcatat tttcagcctg tatggctgtc acttcttttg
     7561 gagaaatagc gaccccttcc tcccagacgc cgtgatcggc acacataacc agtaccgctt
     7621 ttttgcccac atgcggtatg ccattcaccc ccggcattcc tgccagttgt atggcaagcg
     7681 cctccagctt tcctaggcta ccaacaggtt tgagtaaccc gtcaacatgc cgttgtgcac
     7741 gcgacatagc ggcagaattg atggctggga tcgtattcag taaatcggca attatttgca
     7801 tgtcatgtct ctttatagca gtgccagcag gaagaccagt tcaccaagct ctatggctgc
     7861 tcccagcgta tcgcccgttt gcccgcccag cgtacgtttg agaagctgac cgaggatgaa
     7921 aatcgccacc atcgtcacca ccatagcagc cacaccatgc atacccagca ataatacggc
     7981 agcaaaaata gcggctaagc cgagtgtgac gcaggtttgt cgcgccaatg aacacattgc
     8041 caagcccttc ttcccgcgca taacgatgac gatacatcaa taatgcggca gtcccgcggc
     8101 tgaccgcaca tgctgccgcc agcgatgcaa ggatcggttc gccacgcaga gccaactcgc
     8161 ttaacaccag aatctttgcc agtaccacaa aaatcaatgc cagaccgccg tgggtgccta
     8221 aacggctatc acgcatgatt tccaacatgc gatcgcggct acgtgcagaa aatacgccat
     8281 cacaggtatc agcgaggccg tccagatgga attccccggt catcagcgcc agcacaagta
     8341 cgctaaacag tgccgccagt ggtgcgccac accatgcctg cagcaccatg aagaccagcc
     8401 cgctaatcgc gccaagcaat aatccaatca aaggaaaagt aataataccg cgagaataat
     8461 gctcgaaatc cagtccctgg gaccagcgac gcggtacagg cagacgcgta ataaatgaga
     8521 gcatcgccca aaataatttg ctcatttgat ttttactcca atacccgaaa ccaccagcca
     8581 tacttcattt gccgcagcgg ccaactgctg atttacccgc ccggcaatat cacgaaaatg
     8641 tcgtgccaga cgactctccg gcacaatccc cattcccact tcgttagtca ctaatacaac
     8701 ctttgcgggg caacgttggc aggcagcaat caacgactga atctcagcat taatcgcctg
     8761 ttccatcgcc tgataatccc attcatcagg gtctttatcg ccgccataat caaacaacag
     8821 attagtcacc attgtggtaa cgcattccag caacacagcc tcattcgggt taatgtctgc
     8881 atgaattaat tcatcaagat gttgccagcg ctccactgtg cgccagtgct ccgggcggct
     8941 ttgccgatga tgttctatcc gtgcagccat ctcatcatca aggatttgcg aggtagcgat
     9001 atacagaacc tgtgaagagt ccccaataag cacctctgca tggcgactct tcccgctcca
     9061 tgcgcctccc gtcaccaaaa tcatcatacc gactcctgat tcaaatgtat gatggtatac
     9121 attgaacaga gcaatggatc ccccaccgtt gctgaccgca aaacatttac gcgattaata
     9181 ctgcgcgtaa tataattaaa aatccagcat tctcaatcca ttacgaaata agatggcatt
     9241 acggaatagc cagactttct cgtgttaacg tccgacaaaa tgcgtcattg acagcggttt
     9301 ctgtggattg acacatttca ttcagataat gaattaatgc tactgccgga ccaaccagca
     9361 aacgggcatt gtgttctgaa atccaggcta ttgattcaac gtcagcgaca gatctgcgct
     9421 gtaaaagaac ttgtaaaaca acgcaataga attatccgga atggtggcga ctatgcacta
     9481 gaacaccaca atttcgctct ctcggtaagg cactgtcaca gcattcaaca gaatgtgact
     9541 tgccagattt attagcgcca ccagatgttt aaccgggtaa ccacgaccca gtcggggata
     9601 tattccatct tctcgctgac atcatcaaga ataaaaaggt ttatcacact cagagcaacg
     9661 ttcttcagca ggcgggataa ttcttcaaca tttaaaagat gtgttattaa aggcatctca
     9721 aattggacag ggctgccggt tttctgttgc aacaccgttg ctctgcttga gacagaactg
     9781 ccatatgcag catctgatac tttgagtttg accaaatcac agccccgtag cttactgtcc
     9841 aaagccatgt tgaacagagc tagatcgcgt gttttacctt ccaactcaag tctaatccgg
     9901 atcccccaga tatgagatat ctgaagtggc cttttttgac cgatggtacg gtctttattc
     9961 cacggtgacg ttttcatatt caaatctcct gcaatgtggg agatttgagt atggttgtct
    10021 ctcaagagcg aaaataagtc aactatcact agttgttgcg catgtacgta ttctgtcaat
    10081 taa
//
LOCUS       DAJTXY010000131        10079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTXY010000131 DAJTXY010000000
VERSION     DAJTXY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.131"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(122..394)
                     /locus_tag="OO566_003461"
                     /pseudo
     CDS             complement(122..394)
                     /locus_tag="OO566_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
     gene            complement(488..1050)
                     /locus_tag="OO566_003462"
                     /pseudo
     CDS             complement(488..1050)
                     /locus_tag="OO566_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1254..1460)
                     /locus_tag="OO566_003463"
     CDS             complement(1254..1460)
                     /locus_tag="OO566_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR6606613.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1567..2130)
                     /locus_tag="OO566_003464"
     CDS             complement(1567..2130)
                     /locus_tag="OO566_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6606614.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3505..5340)
                     /locus_tag="OO566_003465"
     CDS             complement(3505..5340)
                     /locus_tag="OO566_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606615.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5441..5728
                     /locus_tag="OO566_003466"
     CDS             5441..5728
                     /locus_tag="OO566_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6606616.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5700..7229
                     /locus_tag="OO566_003467"
     CDS             5700..7229
                     /locus_tag="OO566_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR6606617.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7399..8616)
                     /locus_tag="OO566_003468"
     CDS             complement(7399..8616)
                     /locus_tag="OO566_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6606618.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8983..9969)
                     /locus_tag="OO566_003469"
     CDS             complement(8983..9969)
                     /locus_tag="OO566_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606619.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9966..>10079)
                     /locus_tag="OO566_003470"
     CDS             complement(9966..>10079)
                     /locus_tag="OO566_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6606620.1"
                     /translation="EISNKPYLGNMLNDFAGVDQQRVMHCRKAFSDAKVFK"
BASE COUNT         3174 a         2295 c         1876 g         2734 t
ORIGIN      
        1 aaggctgggc catactcctc cggcagaagc agaaaaagct tattatgctt ccatcggaaa
       61 cgatgatctg gcagcctgag ttcacagata aaacactctc caggaaaccc ggggcggttc
      121 acttacacca gacatcgtta ttaagtagca gcaccataag ataaggagtg gcatcgttag
      181 ccacagcttc cctactccag aggtaatata aaggggtggg ctcgactgat ttatctttac
      241 gtcgcttaca ctgcaaatgt tcagaaatta gtctatgcag ctcaccagta aaatcagcaa
      301 tcagagaagg aattgcctta ttcataccag ggcaaggtat taatttaaac tgtaataatt
      361 taattttagg atgtgtagca gcagcccgat acagagttgc aaggacacac ttttgccaga
      421 gggcgttaca ggaaagctta atgtttgatt ctgtatacat aataaatcac cttatagtta
      481 caataggtca aaaaccgctg tagccaaagt tacgctggcc tgatgcttta gtgtcgagct
      541 tcgtcaaata atccagacgt accaataagc gctggtactg ctctgggaaa ttaggatcgt
      601 taatatccag gatgtaacgt ccattagcag ggaaatgaac taatccctgg tgatcttcca
      661 gttccaaccc taaggcactg taccaggctc cggtaattaa ccctctcagg gtgtcagagc
      721 gctcatagtt tcccagatgg taataggcgt ctttgttaaa aaaaggcaga cgtgataatg
      781 acatttaaag gactgcgtat attctttcgc ccagataata caaaccgacg ttgcgtaggt
      841 gcgtttgcct tgttgttttt tacggtgttg atctgcatta attttcgcat tcagcgagtt
      901 acggaaacga gatatctctc cggggatcag gttagggaaa caggtaatga tatccccgtt
      961 gtcaatcaca ggagggtaat ggagatcccc ccgtattgct gttattctgg aagtgttttt
     1021 aatattcgta ttgatagttc tcagagatat atcacactct agatatttta attaagtatc
     1081 agttcgatat cggcatattg tacactacct caggtagatg atgagatcag atacagggat
     1141 caactgacgg tgagcgcagt ccccacattt tatccagggc ttactgcata ttcccaacag
     1201 ccattcgtta gccaggtaga cgaataccct gatttgccgc tggttttgct ttcctatcga
     1261 agggcccata catcatcacg cccacgaaac aaccgacgaa ataacgcaac tttctcatcc
     1321 gtggataata cgaaaacgca ctgcacaggg ctctgcggtt tacgtcgcca ttcaatccca
     1381 tgagcttcaa gtaatgagac cagccgggta ttttctgccc gcagtactgc tagttcatcg
     1441 ctgtcagatt tataaaccat atgcgttatc acttgtacct gtctcccggc ggcaccacga
     1501 ctgaaattcg tgtgaaggca aatccacgcg ataacttacg atagttcgga cataagcccc
     1561 cataattcag ttatgtcgtg gtcgaccccg gttacttttt tcgggaagcc tgctacggcc
     1621 cagaataata ccgtgttctt tctgatcctg tttagccaga tagctaaggg cgtaacgtaa
     1681 gtcgtctacc gcagatttat cactatagtg aatcacgtga tcgatacgca ccgggtattt
     1741 gtctttagcc cggcacagat gaaaataacc ctccccttcc gtgatccgcc tccagatatc
     1801 tcccagttgc ctggatatct gataagagtt cttgtgacgt tgtccatcca ggtagccgat
     1861 aaaatgaata tgaagccctt tgtctggtgt atattccatt acccagtaat atcccgccaa
     1921 catcgtctgg gtttcgctaa gtaaacggta tatttccata cacatactgt gcttacagga
     1981 atgcccgaaa ctgggcgtgt ctttcctgta agcaaaatca attctgaaag gtaacagttt
     2041 agaaaaacgt tgaaacatac catccatgtg ttcattcacg tctttcagaa tcatgaaatc
     2101 cattttgtaa ttgggattgg cattatacat tttatagtcc ttactttata aaactcacag
     2161 gaagagagaa ttacgtaaat actgagagta gagaaacgcc gtaccacggc attaataaac
     2221 gaagtaaacc agcagcacag acaacctgat aaaactgttt tgtattaccg cattactgtg
     2281 atgaggtaat acttcagaat acagtgagac tctcttaaac tgagaaccag tacgaattac
     2341 aatggaaaaa cacacctcat agaattgagt ttcaggaagc accactaaat tctttacagg
     2401 aagtaatctg acattcacaa aactcagtat cggctagcta caggcaacat cgctattaat
     2461 attgtcgaac cattaagtaa agccgagaga gaccacgggt atttaatcat tagcataaaa
     2521 gaataatggt atacagaaca tacccacaca ccataagaag attgttccat acagaaaaga
     2581 tcatatttac ctatacctat aaactgtaac ggagtcataa cacgctctaa tagtgttccg
     2641 atacgtaacc agtgttaagt acgtacagga caacattaca cacataattc accatacagc
     2701 ttcccccata tgcttcactg cgtagtgtat ggagatatat gcattacaca catacagaat
     2761 acttagcata aagaggagtg acattaccac gtaaagagat aagttataca acaaacatac
     2821 aaacaatacg gcacagacac acaataccat aactccaact aataatacta attaacctta
     2881 attatttatg gtatatgaaa taaatgtgat tctcccggaa ctaactaacc taaccagaca
     2941 gatgatcact gtataaaaac ttcagttaat actcatttgt tttgtcgact cagtatataa
     3001 tgagtgacca gtaatcagga taatcacact tacataacct gtaaaaaaga acaagaagaa
     3061 taagccacta acataatgca aaatggcatc tgaatcgatc gcagtttggc attcgaatcg
     3121 atcatgattt ggcccaatcc gtgatcgaat agagcggaat acgcaacata attcaccaga
     3181 caagagctta caccgaatta cagattatgt attaatgtca aaactggcat attttacaga
     3241 tacaatgaac atgcaaccca tcaatctaat aggggcggta tgtacatctg cccatgaatg
     3301 ggatggcgct aagaatacaa aacaattaga catagatcac aaaaacacac cattctaatt
     3361 gaaaaacaaa acaaacaaat aaatcatact attatagaat tttctcttgc cagacataga
     3421 taatacccct gctaattttg ctaatggtta gcaaaattag cagggtatat aaagattatt
     3481 gacccaaaac acactcagat gaattcacga cagctcagga gggttattcc tgatgtcatc
     3541 ttcacaaata atggttaacg catcatatat cattctgcgt ctgttcctgt tttcggcctt
     3601 atcgccatac aacatcatca catttttaat ttcatcgacc ggtgatttca catagtgaac
     3661 taatacacca agcttctcaa cacattgcag taaagtatat ttcttactgc ctgtgaaaag
     3721 ccctatcata gtttctttac gttcgcgact ggataatact ttgttttcca taatgacttc
     3781 tgccagcgtc ttaccatcat aaacatactc tttatctatc agaatattta tcttatccag
     3841 caccttctct acgtcatcaa gcaaacctgt aatatatggg ggcgcaatat atggtttcgt
     3901 actgatcttt tcaagcatac aattatacat cttaataaca cgcatcaggg taaaattgtt
     3961 cgcatcatac tgtatttcac cctccttata tacaggatca ataagtggta ttttttcaat
     4021 aaatgcatcc tgataggctt ttacatggtc aagccaataa aacaacgagc catactttat
     4081 attttttcta tttactttaa cctccagtgc tgtccgaagc aacgataatg cccccctgac
     4141 atatcctgaa ggtaattcac caaacaatcc ttttctttcc atgtcatcat aatgctgcag
     4201 cacatcatta attttatttt tccttatcag acaaatgtac accgcaagtg tttcgtagca
     4261 gctggtcaca tgaacagcct cattctgcaa ctgcccaaaa tagcttataa tctttttcag
     4321 atatgcgtca tcacaaccat tcttatcatt aatattaaat acaagatctt tcagacggga
     4381 aatatattcc tgagatttat catcaagtga aacactataa cgagacaatg ttccttctgc
     4441 actgttttga aacttataaa acatttccag taattttttt gcataaggcg ttgtatcaaa
     4501 gctggtaaag tcataaagat attgcgcagc gtaaccatac aaaccatgaa tccagagtcc
     4561 ttctctcacc aacactctga gataatatgc aaggctttga agtatcttcg tctttcgttg
     4621 tatatcaagt aagacactta atgaataaaa tgcattatag aatttatcaa tattcctgag
     4681 cactttctcc agttcatcct tatccagctt cttctcccgg taatcctttt tatattcatt
     4741 tttgatagcc atgatatatt gttgcatcag gctcttgctt ttcccgttat ccttccgtat
     4801 agttcctgta tatttcccca gagcatacag ctttccattt tcatgaaaat cttccttgtt
     4861 gaatcccagt tcagccagat attcccgaag ataacgttta acagcagctt catcgctttc
     4921 tgtatcagcg tgatcaagac aaggacaata cgatttctgt aaaaaattat aaatatgata
     4981 ataaaaagca gagtcattct ctggtctgta tgtattatac atatctacaa caagaaaaat
     5041 aaccccaaaa gtgcgcagac aatagctgcg aaggaattca ggttttagtg tggaatgcac
     5101 tggataagaa ttcatcatgt aataaaaatg ttcatatcgg gcatatatga tccgccgttg
     5161 ctcctgactg aacacccctt cagcatcaat aatacgactg atctcattat aacgttgatc
     5221 ccagaatttt tgatgcattt caaaattggt actcttccgc ttgtgtgccg gattctcgcc
     5281 ttccttaaac aaccccaaaa cagtacaaat atcatacaac atatttgtaa atgtgcgcat
     5341 atgctgtgct ctccggttgg tttgcatttt atcgcagagg acattctgag cttaacgtat
     5401 caaactgaca caacgacatg caatgcatat ggagaacaat atgtcaaatc aaaccggaca
     5461 gctttacacg ccttcacgca tcctgcgtat ggctgaactt acccgactgc tgggtatatc
     5521 aagatctact atttacgtga aaataaatcc ggcctcgaaa taccacgacc ctacctttcc
     5581 caaaccaata aggcttggta gtgcatccgt aggctggcgg gcatctgcca tcgatgagtg
     5641 gctgatgtta catacggcac cagcctggtc tgaacctgaa aacaaactaa aggaacttaa
     5701 tgatgacgac aaaactatca ctccctgaac ttgacacaac taaactgcct gacaggtgcc
     5761 aggcattact tgatgaaatg catgaagaaa caggaatacg ccgtgaaata cttctgtctg
     5821 ttatgctgac ggtcatggct gcctcggtcc aggatacgca tgaagttgaa ctttccggag
     5881 ggcaacgtac cagccttcag atatttatgt gtctttcatc agcatcaggc agtggaaaaa
     5941 cctctgcctg cgcaaaatta atcgctcctg ttcatgaaac agaagaagaa ctacatcagg
     6001 catatatcga cgataaaaaa aactatgacc gaatgatgga aatgtggaca acagataaaa
     6061 aaatcctgga gcggagatat aaaaaggaaa ttgaaagatc cccggaaaat gctgctgctg
     6121 cacgagcagc acttgaaaag tgtatagcaa ataaacccga acctcccgta caacaggttc
     6181 tcatcgtgaa tgacgcgaca ccagaaggga tagctttgaa actcagccag tcaccctctc
     6241 ttctgttgct gtctgatgaa ggaggtacta ttctggataa gcgtttcgaa cgcaaatcgg
     6301 cactgtacaa cacattatgg agtgggcaac cagtcaacgt tgaacgggca tccagacccg
     6361 ggttccggct gaatgatgtc cgtctcacta tacttatact gactcaaccg gtaatattta
     6421 ataagttttt tactctcact ggcgatcaga tccggggcaa tggctttctg gcccggttgc
     6481 tattttgcga acccggagca attaaaataa tgacgacaga accgcatact gccactcctg
     6541 ttgtgacgca acaatgtgca agctgttttc gtaagaaaag ttttggcagt cagatcagag
     6601 actctctgag agcatccaga gaaagacgtg caaaaagtaa agagcgcatc tgcatgacat
     6661 tatcaaatgc agcatcccgc gcactggagc gctttcacaa agaaaatatg gatactgtca
     6721 gacagaatcc tcatatgggt acatttgaag acattattgt cagaaaaaga gagcaggttg
     6781 tcaggatagc agcacttctg gaactggaga aagatccaga ctcgacagta ataacgctgg
     6841 aaagtactaa tagcgccatt tatctcattg aattttattt caagcatctt atatataaac
     6901 ttgaatccct tcgggaaata tccccggctg agaaacttga taaatggctt cagaaaagaa
     6961 ttatcacaac agcaggttac atattccaga aaagttatat tttacaatat gccccatatg
     7021 ctctcaggaa aaaatgtgtt ctggatgaag cacttgacat actggcggaa caaaggaaaa
     7081 taaggattga tgataacctg gttgtttata taggaaacac gattacacca tccgaactgg
     7141 caaaaaaact aaatattcct gcattcgatg ccggagtttt catttgtgat catcaaaaca
     7201 tactgaagta tcaccgaaac agactataga tactatcgga caaaaaataa attatcacaa
     7261 gtgtattatt cgttataata atgaataatc gttatctgag tgcaatgcca ggttttcatt
     7321 acgaactggc attgcattta tttttcaaaa aagaaagcag taaccatttg gctactctga
     7381 gtatgccagt ccatgaaact aagcgaattc cttagtgttt ttagcaagtt gagacatcac
     7441 ataatccgcc catgcctgca tcatcggtac acgctgctcc agtagatcag tacgatgata
     7501 tgccgcctca accttatttt tcagcgtatg agcgagcgcc ctttccgcca gatcccgcga
     7561 atacccctgt tcactacacc agtccctgaa tgttgagcga aaaccatgtg ccgtggcaac
     7621 tcgccccgga atgtcactga cggctttctt tttacgcaga aaacttgtca acaccatatc
     7681 ggaaaggatc tgctgctttc tgggtgaagg gaacaccagt tcatcatgca ggccacgtat
     7741 attttccaga atgtaaatag cctgccggga taaaggaaca cgatgctgta gcctagcttt
     7801 cattctttct gcaggtatag tccatacccg cttatgaaaa tcaatttcag cccagcgcat
     7861 tcccctggct tcgcccgagc gagttgctgt aagtatcacc attaataaca gtgcgcgggt
     7921 aacattataa ggttcatcac tatacacact ggtcgccaca aaaagcggta actgcctcca
     7981 gggcattgcg ggttggtgtt catcacgtcc tcttgtctgc tgaggaagca aatggtcaac
     8041 cacatcaaca ggatttgcca cacaaaaacc gtgcgcccat ccccactgca taacaacatg
     8101 aatgcgctgt ttaacccggc ttgccgtttc tgacaaggtt aaccagactg gacgcagtgt
     8161 ttctgccacg tccgcagccg taatcgaatc cagcgttttt gctcccagtt gaggaaacgc
     8221 gtaattctca agcgtcgata accactgcct tacatgcttt ggattctccc atccaggaga
     8281 cagttctgca tgtacacgcc tggctgcatc ggcaaatgtt gggataacga ctttctcaga
     8341 ttcagccttt ttaatctcca gaggatcatc acctgcagca agttgctctc gcattatccg
     8401 ggcagtacgt gcagcttcag caatactgac ctctgggtaa gttcccaatc cagcattacg
     8461 tcttttttgt gtcaccggac ttacataacg aaaaacccat ttcccccgcc cctttactga
     8521 agaaggatga agggtcagtc cggtaattcc cccatggggc aatggtttgt catcaggttt
     8581 gatatgtctt gctttcgtat ccgtcaatac tgccatacgc taattcccgt cactctggta
     8641 tgccatccag tatgccatta gtccctcgct tcactaagaa tacattagat aacatcggac
     8701 aacaaaaagt gtaatacatt gtttataaag aaattatata acacccctgg attaagtcag
     8761 atttatttca ggcggtcccc ctcaccgcca tatttaaaga agagcgcgta cgaaagtacg
     8821 ggcttttttt tcgtatattg tacacaccgg gtgcccccgc ccacgtctgt tagggcgagg
     8881 ggaaattgtg cacatcaact gtctggttac tcagtcaaca atcaaaatgc gcaagatagt
     8941 acagaacata tatgcgttat ctggtcactt cctgtaactt cctcacttat taaaatgccc
     9001 ccaacggaaa tacatgtcac atgccatcat aaaatcgtta tgccagagga atcgacaatg
     9061 gaaaaaatag taaattaagc acggtacata gcagagtaaa aaataggctt caaaccaaat
     9121 ccgccattcc acaagcgttg ccaataccga caccagcgta atccacatca tttcaataac
     9181 cagcaatatg aataaccgaa gccaaagcgg cttggcaaaa aaggtcgaca acgtattttc
     9241 actggagaaa aacagatcga tttcctgcaa aaaaaacctg cccagaaaag cgtaccgctt
     9301 ggagaagcgc agaaaaaaca gattaatcgc caacactacg ccacaaagaa tgagcaatgc
     9361 aattatcgat ttaaacgtta accactgccc ctctgtcaca aaagaatcac tgttacgcaa
     9421 cgggctgata gtaagcaggt taacaaccgt tgagatgcac accgaaagca gcacatagcg
     9481 agtcagactt ttatatttta cttttttcgc tactgaaata gcgacctgct tgacataact
     9541 ggtgcggatc atccgaaaca gcagaaagat gaccaacggc ccaagtaacg aaatcaggaa
     9601 cagcgcatat aacgagggta gaagctggta tagcccccaa ctcattgcac tcaacatgat
     9661 taacgacaga aatcctgcca ccagaggttg tgtaagaaat aattctattt tgtggttctg
     9721 ctttttctta aaccacaaaa aatcgatatt cttcgtgacg catttataag cccagtacgc
     9781 cttgagatca aaaataaaat tatacagcac cagggccaga atcccactaa ctacaccaat
     9841 atcctctgat ggaaaagtaa accctgcatt tttccaataa aagcctatca gaataagtcc
     9901 aatagcataa atgtatatca gatttcgttt atcctgaaag cgaataaaag ttaaataatc
     9961 aggaatcatt tgaatacctt tgcatcacta aatgctttac gacaatgcat aactcgttgc
    10021 tgatccacgc cagcaaaatc atttaacata ttccccaaat agggcttatt acttatttc
//
LOCUS       DAJTXY010000132         9946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTXY010000132 DAJTXY010000000
VERSION     DAJTXY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.132"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(47..1330)
                     /gene="aroA"
                     /locus_tag="OO566_003471"
     CDS             complement(47..1330)
                     /gene="aroA"
                     /locus_tag="OO566_003471"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6606621.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1401..2489)
                     /gene="serC"
                     /locus_tag="OO566_003472"
     CDS             complement(1401..2489)
                     /gene="serC"
                     /locus_tag="OO566_003472"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6606622.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2688..3316)
                     /locus_tag="OO566_003473"
                     /pseudo
     CDS             complement(2688..3316)
                     /locus_tag="OO566_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3446..5206
                     /locus_tag="OO566_003474"
     CDS             3446..5206
                     /locus_tag="OO566_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6606623.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5612..6469
                     /gene="focA"
                     /locus_tag="OO566_003475"
     CDS             5612..6469
                     /gene="focA"
                     /locus_tag="OO566_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6606624.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6524..8806
                     /gene="pflB"
                     /locus_tag="OO566_003476"
     CDS             6524..8806
                     /gene="pflB"
                     /locus_tag="OO566_003476"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6606625.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            8998..9738
                     /gene="pflA"
                     /locus_tag="OO566_003477"
     CDS             8998..9738
                     /gene="pflA"
                     /locus_tag="OO566_003477"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6606626.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2554 a         2492 c         2371 g         2529 t
ORIGIN      
        1 acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag gctgcttggc
       61 taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat ttgggatcaa
      121 gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc atccggtgat
      181 cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata cgaatgtaat
      241 cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc gcaaacaggc
      301 gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg ccttttgcaa
      361 ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata tccatatcaa
      421 tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa atggtcgcgc
      481 ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta cgtccaatac
      541 cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa gaagccgaag
      601 atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg ccttttacga
      661 caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc agattgagtg
      721 tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc gtatcttccg
      781 gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg gagccatcaa
      841 cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa ttttcttgtt
      901 ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga ccaatcgggc
      961 gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga caaagagctg
     1021 ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg gcaccttctg
     1081 cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct gaaagcgtat
     1141 agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg ctatccagca
     1201 gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg cggttagaaa
     1261 cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt tgtaacgtca
     1321 gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc tggctgtggg
     1381 gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat gaagtctgtc
     1441 agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat tccgccgacc
     1501 acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag gaacaatttg
     1561 tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt agctttcgcc
     1621 acatcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc tgctttttgc
     1681 tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt aaagaccaga
     1741 ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt atcgttgagg
     1801 atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa atcttcacga
     1861 acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc gtaaattaca
     1921 ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc ggcggcgacc
     1981 accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat ggtttcattc
     2041 gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat tggcttaacc
     2101 gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca gtattttttc
     2161 gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc ggtggtttta
     2221 tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc atggcagaat
     2281 aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc ggcttcctct
     2341 gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt accaagaccg
     2401 ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag cattgccgga
     2461 ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt gctattaacc
     2521 cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc ggttttatca
     2581 ttcagtgaca cacatcgcaa tgagtaaaac cgttgagagc aaaaatgtgg cttgcatcac
     2641 acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca ggtcacccgt
     2701 tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa ttgtttttcc
     2761 cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc cgactccggc
     2821 accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg tttcaccttg
     2881 tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc cagacgtacc
     2941 tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa attccgtcat
     3001 gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccagg cggtataaca
     3061 acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca taaaacgcca
     3121 catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc gacatctgtc
     3181 gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag gtataaagac
     3241 tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc atacggtgga
     3301 gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag catagtcaac
     3361 agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat tattgcgcgc
     3421 tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca aagatgccgc
     3481 tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct ttcagattga
     3541 agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc gcgacaaaga
     3601 gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc tggcttctgc
     3661 actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt tctggctggg
     3721 cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt tcccactgag
     3781 cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat tttacgatcc
     3841 ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg aagatcgtgg
     3901 tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata ttccgatgaa
     3961 tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc gcaacgaagc
     4021 acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca ttattgctga
     4081 aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag cggtggttga
     4141 agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg gttcccttgg
     4201 cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca cctgctttgc
     4261 ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga ccgagctgct
     4321 gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg atgatgaaga
     4381 agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt taatctcctg
     4441 ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct ccggcaccac
     4501 ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag aagtttatat
     4561 tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg gcatgtccga
     4621 tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc atttacgtga
     4681 aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga acctcatcga
     4741 gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt tgggtctggc
     4801 gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag ccatgctggc
     4861 gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga tggagtttaa
     4921 ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa cgttgttatt
     4981 actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg ttcgcatgta
     5041 cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg cgttttacgg
     5101 cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt tgttgaaggc
     5161 ctacgaaaag ctgcaacgcg ccaaagcagc attctgggca aaataaaatc aaatagccta
     5221 cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt taatgccccg
     5281 ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata tcggtaattt
     5341 gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc ttttaattaa
     5401 ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta aaatttattt
     5461 ttatttggat aatcaaatag ttactccgta tttgcataaa aaccatgcga gttacgggcc
     5521 tataagccag gcgagatatg atctatatca atttctcatc tataatgctt tgttagtatc
     5581 tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt ttgatctttt
     5641 acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag caacgaaaca
     5701 tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa tcgcattcgt
     5761 cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg caaaactggt
     5821 tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag ccgatctctt
     5881 tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct ggggtcagtt
     5941 ggctaaaaac tggctaaatg tctattttgg caatctggtc ggcgcactgc tgtttgtact
     6001 tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac taaacgtcct
     6061 acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc ttggtatcct
     6121 ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca gcctgatgga
     6181 caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt ttgagcacag
     6241 tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg catctccgga
     6301 attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg tgatgaactt
     6361 catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg gtttgttggt
     6421 tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat ggttgtcgaa
     6481 gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg agcttaatga
     6541 aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg aagtaaacgt
     6601 ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct tcctggctgg
     6661 cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta aactggaaaa
     6721 ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca cctctcacga
     6781 cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg aagctccgct
     6841 gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct gcaaagcgta
     6901 caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta aaactcacaa
     6961 ccagggcgta ttcgacgttt acactccgga catcctgcgt tgccgtaaat ccggtgttct
     7021 gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc gtcgcgttgc
     7081 gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct ctctgcaggc
     7141 tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag aaatcgctga
     7201 acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct acgacatctc
     7261 tggtccggca accaacgctc aggaagctat ccagtggact tacttcggct acctggctgc
     7321 tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt acctccacct tcctggatgt
     7381 gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc aggaaatggt
     7441 tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg aatacgatga
     7501 actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg gcctcgatgg
     7561 tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca ctatgggtcc
     7621 gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga acttcaagaa
     7681 attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg atgacctgat
     7741 gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc cgatgatcgt
     7801 tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca tgctgtacgc
     7861 aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt ctgaaccgat
     7921 caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact tcatggactg
     7981 gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg acaagtacag
     8041 ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca tggcgtgtgg
     8101 tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg cgaaagttaa
     8161 accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg aatacccgca
     8221 gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag aacgtttcat
     8281 gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt ctgttctgac
     8341 catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg gtcgtcgtgc
     8401 tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga aaggtgctgt
     8461 agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg gtatctccta
     8521 caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta agaccaacct
     8581 ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg gtcagcacct
     8641 gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc cggaaaaata
     8701 tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc tgactaaaga
     8761 acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag atttgactga
     8821 aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt ttttagcacg
     8881 agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga ctcgacgtag
     8941 ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa caccgcaatg
     9001 tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg cccaggtatt
     9061 cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca taaccgcgac
     9121 acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa ggaagtggtg
     9181 acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg cggtgaagca
     9241 atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga aggcattcat
     9301 acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga tgaactgctg
     9361 gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat ccaccaaaat
     9421 ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc gaacaaaaat
     9481 gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga cgattcggcg
     9541 catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga acttctcccc
     9601 taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa actcgatggt
     9661 gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga gcagtacggt
     9721 cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg ccgctttttt
     9781 attccgcgct tcgttctgct acagcgtttt taacacatct tatagtgcat ctaccatatc
     9841 ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc catcgaatac
     9901 cactgtatta cacaagaaag tgacatcggc atcttcaata ccaaga
//
LOCUS       DAJTXY010000133         9758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTXY010000133 DAJTXY010000000
VERSION     DAJTXY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.133"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..896
                     /locus_tag="OO566_003478"
     CDS             <1..896
                     /locus_tag="OO566_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6606627.1"
                     /translation="AGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPPALVLMQLAW
                     GWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPVKPRPQLQFSEWLAL
                     FKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIPFLAAAVGMW
                     VNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFISMALFCV
                     HFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSFNLALVI
                     AACVTFTGALCYFFIVKDRIE"
     gene            1148..2065
                     /gene="oxyR"
                     /locus_tag="OO566_003479"
     CDS             1148..2065
                     /gene="oxyR"
                     /locus_tag="OO566_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6606628.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2048..3448)
                     /gene="sthA"
                     /locus_tag="OO566_003480"
     CDS             complement(2048..3448)
                     /gene="sthA"
                     /locus_tag="OO566_003480"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6606629.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3769..4419
                     /gene="fabR"
                     /locus_tag="OO566_003481"
     CDS             3769..4419
                     /gene="fabR"
                     /locus_tag="OO566_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6606630.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4419..4778
                     /locus_tag="OO566_003482"
     CDS             4419..4778
                     /locus_tag="OO566_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6606631.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4818..5918)
                     /gene="trmA"
                     /locus_tag="OO566_003483"
     CDS             complement(4818..5918)
                     /gene="trmA"
                     /locus_tag="OO566_003483"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6606632.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6032..6222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6287..8131
                     /gene="btuB"
                     /locus_tag="OO566_003484"
     CDS             6287..8131
                     /gene="btuB"
                     /locus_tag="OO566_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6606633.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALDHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8076..8933
                     /gene="murI"
                     /locus_tag="OO566_003485"
     CDS             8076..8933
                     /gene="murI"
                     /locus_tag="OO566_003485"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6606634.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            9307..>9758
                     /locus_tag="OO566_003486"
     rRNA            9307..>9758
                     /locus_tag="OO566_003486"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2264 a         2348 c         2644 g         2502 t
ORIGIN      
        1 ctgcgggagt aaagtcgatc accgactggt atgcacaaaa agaacgcggc acggcgctgg
       61 ggatttttaa ctcatctacc gttatcggtc aggctatcgc gcctcctgct cttgtattga
      121 tgcagctggc atggggctgg cggacgatgt tcgttatcat cggcgtggca gggattctgg
      181 ttgggatctg ttggtacgcg tggtatcgca accgggcgca gtttgtcctg actgacgaag
      241 aacggacgta tctctccgcg ccggttaaac cgcgtccaca gctgcaattt agcgagtggc
      301 tggcgctgtt taagcatcgg acaacctggg ggatgatttt aggtttctct ggcgtcaatt
      361 ataccggttg gctctacatt gcgtggttac ccggttattt gcaagccgaa caaggtttca
      421 gtctggcgaa aaccggttgg gtagcggcga ttcctttcct cgcggcggcg gtcgggatgt
      481 gggttaacgg tattgtggtc gatcggctgg cgaaaaaagg ctacgacctg gcgaagacgc
      541 gtaaaacggc tattgtctgc ggtttgatga tgtcggcatt aggcacgttg ctggtcgtgc
      601 aatcttcctc gccagctcag gcggtagcgt ttatctcaat ggcgctgttc tgcgtgcatt
      661 tcgcgggaac gtctgcatgg ggactggtgc aggtgatggt gtcagaaaca aaagtggctt
      721 ccatcgccgg gattcaaaac tttggcagtt ttgtttttgc ttcctttgct ccgatcgtaa
      781 ccggttgggt agtggatacc acacactcgt ttaatctggc gctggttatc gcggcctgtg
      841 tgacgttcac cggggcgctg tgttacttct ttattgtcaa agatcgcatt gagtaatcgt
      901 tactatcagg ctctcttgct gtgggcctgt agaataaaaa aaagcggatc ctggagatcc
      961 gcaaaagttc acgttggctt tagttattcg agttgagaaa ctctcgaaac gggcagtgac
     1021 ttcaagggtt aaaagaggtg ccgctccgtt tctgtgagca attatcagtc agaatgcttg
     1081 atagggataa tcgttcattg ctattctacc tatcgccatg aactatcgtg gcgatggagg
     1141 atggataatg aatattcgtg atcttgagta cctggtggca ttggctgaac accgccattt
     1201 tcggcgtgcg gcagattcct gccacgttag ccagccgacg cttagcggcc aaattcgtaa
     1261 gctggaagat gagctgggcg tgatgttgct ggagcggacc agccgtaaag tgttgttcac
     1321 ccaggcggga atgctgctgg tggatcaggc gcgtaccgtg ctgcgtgagg tgaaagtcct
     1381 taaagagatg gcaagccagc agggtgagac gatgtccgga ccgctgcata ttggtttgat
     1441 tcctacggtt ggaccatacc tgctaccgca tattatcccg atgctgcacc agacatttcc
     1501 aaagctggaa atgtatctgc atgaagcaca gacccaccag ttactggcgc aactggacag
     1561 cggcaaactc gattgcgtga tcctcgcgct ggtgaaagag agcgaagcat tcattgaagt
     1621 gccgttgttt gatgagccaa tgttgctggc tatctatgaa gatcacccgt gggcgaaccg
     1681 cgaatgcgta ctgatggccg atctggcagg ggaaaaactg ctgatgctgg aagatggtca
     1741 ctgtttgcgc gatcaggcaa tgggtttctg ctttgaagcc ggggcggatg aagatacaca
     1801 cttccgcgcg accagcctgg aaactctgcg caacatggtg gcggcaggta gcgggatcac
     1861 tttattgcca gcgctggctg tgccgccgga gcgcaaacgc gatggggttg tttatctgcc
     1921 atgcattaag ccggaaccac gccgcactat tggcctggtt tatcgtcctg gctcaccgct
     1981 gcgcagccgc tatgagcagc tggcagaggc catccgcgca agaatggatg gccatttcga
     2041 taaagtgtta aaacaggcgg tttaaaccgt ttaacgcagc tacccgatag gcttccgcca
     2101 tcgtcgggta gttaaaggtg gtgttgacga agtactcaat agtgttgccg ccacctttct
     2161 gttccataat cgcctgaccg atatgaataa tttcggcagc gcgctcgcca aagcagtgaa
     2221 tacccagaat ctcttttgtt tcccgatgga acaaaatttt cagcgtgccc acgttcatgc
     2281 cgacgatttg tgcacgtgcc agatgtttga actgggcgcg gcccacttca tacggcactt
     2341 tcatcgcggt cagctgctgt tcggttttgc ccacagagct gatttccggg atggtgtaaa
     2401 tgccggtagg gatatcttca atcagatgtg cggtggcttc gccttttacc agcgcctgcg
     2461 cggcaatgcg cccctggtca taggccgctg atgccaggct cggataacca atcacgtcgc
     2521 ccaccgcgta aacgtgcggc tgtgcggtct gatacatgct gttgaccttc agctgtccgc
     2581 gactgtcagt ttccagccca atgttctgta acgccagcga atcggtatta ccggtacgac
     2641 cgttggcata gagcaggcag tcagctttca gttttttacc cgacttcaga tgcatgatca
     2701 caccgtcgtc acagctttcg atcttctcgt actcttcgtt gtgacgaatc actacgccac
     2761 tgttccagaa gtgataggag agagaatctg acatctcttg atcgagaaat gccagcaggc
     2821 gatcgcgggt gttgatcaga tccactttta catccatacc gcggaagatc gacgcatatt
     2881 cacagccgat cactccagca ccatagataa gtacatggcg cggttcgtgg tgcatgctaa
     2941 gaattgagtc gctgtcgtaa atgcgtggat gggtgaaatc aacatctgtt ggatgatatg
     3001 gacgagagcc gcaggcaata acaaattttt cagcggttag tgtttcaacg ctgccgtccg
     3061 ggcaatccag cgccaacgta tgctcgtcaa caaagcgagc gttcccctgc aatattttac
     3121 agtgattacg ttcgtaaaat ccctgacgca tgcgcgtttg ttgattaatc acgttatcgg
     3181 catggttaag gatatcggca aaagaagagc ggagcagtcg ggaatggtcg ctgtatagtg
     3241 ggttttgatt gaattctata atgcggctga cggcgtgacg gagagctttc gacgggatgg
     3301 tgccccagtg ggtgcaaccg ccgccaacat tttgataacg ctcgataact gcgacgcgcg
     3361 caccttgctt aaccaggccc attgcagcgc cttcgccgcc ggggccggaa cctattacta
     3421 tggcatcgta atcgtaggaa tgtggcatgg tagggcttac ctgttcttat acataaaatc
     3481 aacataatgg taacattcta tcgcgggtaa gccaattgat ccccgtcatt tatctggcta
     3541 tatcctgagc ggcctttgct ttgtctgttt cttacttttg ccctgacgtt ttattggatt
     3601 tttatcgacg atactctccg tttaagcggc aggtttccgc tgtacgtaaa agaaccggcc
     3661 agtaaatatg ttttattgcg ttaccgttca ttcacaatac tggagcaatc cagtatgttc
     3721 attctctggt atagtgccag cagtactttt ggcaaggatt cagacatcgt gatgggcgta
     3781 agagcgcaac aaaaagaaaa aacccgccgt tcgctggtgg aagccgcatt tagccaatta
     3841 agtgctgaac gcagcttcgc cagcctgagt ttgcgtgaag tggcgcgtga agcgggcatt
     3901 gctcccacct ctttttatcg gcatttccgc gacgtagacg aactgggtct gaccatggtt
     3961 gatgagagcg gtttaatgct acgccaactc atgcgccagg cacgtcagcg tatcgccaaa
     4021 ggcgggagtg tgatccgtac ctcggtctcc acatttatgg agttcatcgg taataatcct
     4081 aacgccttcc ggttattatt gcgggaacgc tccggcacct ccgctgcgtt tcgtgccgcc
     4141 gttgcgcgtg aaattcagca cttcattgcg gaacttgcgg actatctgga actcgaaaac
     4201 catatgccgc gtgcgtttac tgaagcgcaa gccgaagcaa tggtgacaat tgtcttcagt
     4261 gcgggtgccg aggcgttgga cgtcggcgtc gaacaacgtc ggcaattaga agagcgactg
     4321 gtactgcaac tgcgaatgat ttcgaaaggg gcttattact ggtatcgccg tgaacaagag
     4381 aaaaccgcaa ttattccggg aaatgtgaag gacgagtaat gaaacaagca aatcaagata
     4441 gaggtacgct gctgctggcg ttggttgctg gcttatcgat taatggtact ttcgcagcgc
     4501 tgtttagctc cattgtgcca ttttctgtat tcccgattat ttccctggtg ctgacggttt
     4561 actgcctgca tcaacgttat cttaatcgca ccatgtcggt aggcttgccg ggtctggcag
     4621 ctgcctgttt tattctcggc gtactgctgt acagcacggt agttcgtgcg gaatatccgg
     4681 atatcggctc taacttcttc ccggcagtac tctccgtcat tatggtgttc tggattggcg
     4741 cgaagatgcg taaccgtaag caggaagttg ctgagtaatc ggtatcatgc cggatgctgg
     4801 cgcatccggc atgggtttta cttcgcggtc agtaatacgc cgcactccat atggtgcgtg
     4861 taggggaact gatcaaacag agccagacgt tcgaccttgt gcgtctggct taatgtttcc
     4921 agatttttgc ataacgtttc cggattgcag gagatataca gaatacgtgg atacgcctgc
     4981 accattttct cggtttcact gtccagaccg ctgcgcggtg ggtcgacaaa aatggtttcg
     5041 aactgatagc tctttaagtc gatcccttgc aggcggttaa actcgcgcac accattcatc
     5101 gcctgagtaa attcttctgc cgccatacga ataatttgta cgttatcaat atggttagcg
     5161 gcaatgttgt attgcgcggc agcgactgac ggtttggcga tttcggtggc taatacccga
     5221 tcaaaattgc gcgccagcgc taatgaaaag ttaccgttgc cgcagtacag ctccagtaaa
     5281 tcgccttttg agcctttggt tacgtccagc gcccattcca gcatttgaat attcatcgcc
     5341 gcgttcggct gggtaaagct gttttctacc tgacgataga tcatctcttt ccctgcgacc
     5401 ggcagacgct cgtcgatgta atcttgatcc agcgcgattt tggttttcgt tgcccgacca
     5461 atcagatgca cattcagatt ctgcgcgcgc agtgcatcgc gcagtgcttc cgcttcctga
     5521 cgccactcat catccagctt tttatggtac agcagggaaa ccaccgcctg attactcagc
     5581 gtggtgaggt aatcaatctg gaacaacttg tggcgcagaa cgggattatt acgcacaccc
     5641 gcaatcatcg ccgtcatcaa ctggttgatg agttcactgg cggcggggaa gctatccacg
     5701 cggatgcggc ttttggtttg ttgatcgaaa atgatgtgat acaggtcatc gccatcgtgc
     5761 cagatgcgga actccgcgcg catccggtaa tgactgaccg gcgagcgaaa cacttccgga
     5821 accaggtcag aaaacggtgc catcatactt tgcaaacgta ccactttttc ggctaactgc
     5881 gcttcatact gttctgttgg aaggtgttcg ggggtcatga tgtatcctga aaaattaaag
     5941 tacgcggcga ttgtagggat tgctcatcag atgtccagat cttgatgaat tcctatttgt
     6001 gagctacgtc tggacagtaa cttgttacaa cctgtagcat ccacttgccg gtcctgtgag
     6061 ttaataggga atccagtgcg aatctagagc tgacgcgcag cggtaaggaa aggtgcgatg
     6121 attgcgttat gcggacactg ccatccggtg ggaagtcatc atctcttagt atcttagata
     6181 cccctccaag cccgaagacc tgccggccaa cgtcgcatct ggttctcatc atcgcgtaat
     6241 attgatgaaa cctgcggcat ccttcttcta ttgtggatgc tttacaatga ttaaaaaagc
     6301 ttcgttgctg acggcgtgtt ccgtcacggc attttccgct tgggcacagg ataccagccc
     6361 ggatactctc gtcgttactg ctatccgttt tgaacagccg cgcagcactg tgcttgcacc
     6421 aaccaccgtt gtgacccgtc aggatatcga ccgctggcag tcgacctcgg tcaatgatgt
     6481 gctgcgccgt cttccgggcg tcgatatcac ccaaaacggc ggttcaggtc agctctcatc
     6541 tatttttatt cgcggtacaa atgccagtca tgtgctggtg ttaattgatg gcgtccgcct
     6601 gaatctggcg ggggtgagtg gttctgccga ccttagccag ttccctattg cgcttgtcca
     6661 gcgtattgaa tatatccgtg gaccgcgctc cgctgtttat ggttccgatg caataggcgg
     6721 ggtggtgaat atcatcacga cgcgcgatga acccggaacg gaaatttcag cagggtgggg
     6781 aagcaatagt tatcagaact atgatgtctc tacgcagcaa caactggggg ataaaacacg
     6841 ggtaacgctg ttgggcgatt atgcccatac tcatggttat gatgttgttg cctatggtaa
     6901 taccggaacg caagcgcaga cagataacga tggtttttta agtaaaacgc tttatggggc
     6961 gctggatcat aactgtactg atgcctggag cggctttgtt cgcggctatg gctatgataa
     7021 ccgtaccaat tatgacgcgt attattctcc cggttcaccg ttgctcgata cccgtaaact
     7081 ctatagccaa agttgggacg ccgggctgcg ctataacggc gaactgatta aatcacaact
     7141 cattaccagc tatagccata gcaaagatta caactacgat ccccattatg gtcgttatga
     7201 ttcgtcggcg acgctcgatg agatgaagca atacaccgtc cagtgggcaa acaatgtcat
     7261 cgttggtcac ggtagtattg gtgcgggtgt cgactggcag aaacagacta cgacgccggg
     7321 tacaggttat gttgaggatg gatatgatca acgtaatacc ggcatctatc tgaccgggct
     7381 gcaacaagtc ggcgatttta cctttgaagg cgcagcacgc agcgacgata actcacagtt
     7441 tggtcgtcat ggaacctggc aaaccagcgc cggttgggaa ttcatcgaag gttatcgctt
     7501 cattgcttcc tacgggacat cttataaggc accaaatctg gggcaactgt atggcttcta
     7561 cggaaatccg aatctggacc cggagaaaag caaacagtgg gaaggcgcgt ttgaaggctt
     7621 aaccgctggg gtgaactggc gtatttccgg atatcgtaac gatgtcagtg acttgatcga
     7681 ttatgatgat cacaccctga aatattacaa cgaagggaaa gcgcggatta agggcgtcga
     7741 ggcgacagcc aattttgata ccggaccact gacgcatact gtgagttatg attatgtcga
     7801 tgcgcgcaat gcgattaccg acacgccgtt gttacgccgt gctaaacagc aggtgaaata
     7861 ccagctcgac tggcagttgt atgacttcga ctggggtatt acttatcagt atttaggcac
     7921 tcgctatgat aaggattact catcttatcc ttatcaaacc gttaaaatgg gcggtgtgag
     7981 cttgtgggat cttgcggttg cgtatccggt cacctctcac ctgacagttc gtggtaaaat
     8041 agccaacctg ttcgacaaag attatgagac agtctatggc taccaaactg caggacggga
     8101 atacaccttg tctggcagct acaccttctg aaccacgtcc caccgtgctg gtgtttgact
     8161 ccggcgtcgg tgggttgtcg gtctatgacg agatccggca tctcttaccg gatctccatt
     8221 acatttatgc tttcgataac gtcgctttcc cgtatggcga aaaaagcgaa gcgtttattg
     8281 ttgagcgagt ggtggcaatt gtcaccgcgg tgcaagaacg ttatcccctt gcgctggctg
     8341 tggtcgcttg caacaccgcc agtaccgttt cacttcctgc attacgcgaa aagttcgact
     8401 tcccggttgt tggtgtcgtg ccggcgatta aacctgctgc acgtctgacg gcaaatggca
     8461 ttgtcggatt actggcaacc cgcggaacag ttaaacgttc ttatactcat gagctgatcg
     8521 cgcgtttcgc taatgaatgc cagatagaaa tgctgggctc ggcagagatg gttgagttgg
     8581 ctgaagcgaa gctgcatggc gaagatgttt ctctggatgc actaaaacgt atcctacgcc
     8641 cgtggttaag aatgaaagag ccgccagata ccgttgtatt gggttgcacc catttccctc
     8701 tactacaaga agaactgtta caagtgctgc cagagggaac ccggctggtg gattctggcg
     8761 cagcgattgc tcgccgaacg gcttggttgt tagaacatga agccccggat gcaaaatctg
     8821 ccgatgcgaa tattgccttt tgtatggcaa tgacgccaga agctgaacaa ttattgcccg
     8881 ttttacagcg ttacggcttc gaaacgctcg aaaaactggc agttttaggc tgatttggtt
     8941 gaatgttgcg cggtcggaaa attattttaa atttcctctt gtcaggccgg aataactccc
     9001 tataatgcgc caccactgac acggaacaac ggcaaacacg ccgccgggtc agcggggttc
     9061 tcctgagaat tccggcagag aaagcaaaaa taaatgcttg actctgtagc gggaaagcgt
     9121 attatgcaca cctcgcgccg ctgagaaaaa gcgaagcggc actgctcttt aacaatttat
     9181 cagacaatct gtgtgggcac tcgaagatac ggattcttaa cgtcgcaaga cgaaaaatga
     9241 ataccaagtc tcaagagtga acacgtaatt cattacgaag tttaattctt tgagcatcaa
     9301 acttttaaat tgaagagttt gatcatggct cagattgaac gctggcggca ggcctaacac
     9361 atgcaagtcg aacggtaaca ggaagcagct tgctgtttcg ctgacgagtg gcggacgggt
     9421 gagtaatgtc tgggaaactg cctgatggag ggggataact actggaaacg gtagctaata
     9481 ccgcataacg tcgcaagacc aaagaggggg accttcgggc ctcttgccat cggatgtgcc
     9541 cagatgggat tagctagtag gtggggtaac ggctcaccta ggcgacgatc cctagctggt
     9601 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
     9661 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
     9721 aggccttcgg gttgtaaagt actttcagcg gggaggaa
//
LOCUS       DAJTXY010000134         9724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTXY010000134 DAJTXY010000000
VERSION     DAJTXY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.134"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            429..1547
                     /locus_tag="OO566_003487"
                     /pseudo
     CDS             429..1547
                     /locus_tag="OO566_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
     gene            1613..1861
                     /locus_tag="OO566_003488"
     CDS             1613..1861
                     /locus_tag="OO566_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6606635.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLSAIEYFVLRFIWRRWFSLAD"
     gene            1926..2294
                     /locus_tag="OO566_003489"
     CDS             1926..2294
                     /locus_tag="OO566_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6606636.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPRYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2388..3041
                     /gene="nfsB"
                     /locus_tag="OO566_003490"
     CDS             2388..3041
                     /gene="nfsB"
                     /locus_tag="OO566_003490"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6606637.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3149..4396
                     /gene="ybdG"
                     /locus_tag="OO566_003491"
     CDS             3149..4396
                     /gene="ybdG"
                     /locus_tag="OO566_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6606638.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO566_003492"
     CDS             complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO566_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6606639.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5941..9076)
                     /gene="cusA"
                     /locus_tag="OO566_003493"
                     /pseudo
     CDS             complement(5941..9076)
                     /gene="cusA"
                     /locus_tag="OO566_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9088..9629)
                     /locus_tag="OO566_003494"
                     /pseudo
     CDS             complement(9088..9629)
                     /locus_tag="OO566_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT         2390 a         2695 c         2349 g         2290 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagggttg atagaaatat
       61 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca
      121 cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt
      181 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
      241 tcaatcgtca aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag
      301 tagccaaagt gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga
      361 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag
      421 agaaacctat gccattaccc gattttcatt tttctgaacc ttttaccctc ggtattgaac
      481 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      541 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      601 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag
      661 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      721 gcacgccccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      781 gaacgctgga aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc
      841 atgttggctg tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg
      901 tgccgcattt tattgccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt
      961 ttgccccctc acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg
     1021 tcagtaacta gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg
     1081 acagcattaa agatctgcac tgggatattc gccccagtcc tcattttggc acggtggagg
     1141 ttcgggtgat ggataccccg ttaaccctta gccacgcggt gaatatggcg ggattaattc
     1201 aggccaccgc ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc
     1261 tgctgtataa attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg
     1321 atccgcacac tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa
     1381 tcgccccttc agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg
     1441 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc
     1501 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc
     1561 ttacagaaga atatccctga caatggtgtt ttaacttata tttaacaatg ttatgaaaca
     1621 ccctttagaa accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct
     1681 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1741 tctgatggat cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctgag
     1801 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     1861 aacgtcgatt atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaaggaa
     1921 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct
     1981 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2041 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag tccgatccac
     2101 aaaaatccct gttaaatcag caaatatacc caagcattaa gccaaggtat cacatgaata
     2161 aaaagcactg gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2221 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2281 tgcgtccagg ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga
     2341 accttgtaat ctgctggcac gcaaaattac ttacacatgg agtctttatg gatatcattt
     2401 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2461 aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc
     2521 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2581 ccggtaatta tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2641 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg
     2701 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2761 ccgatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     2821 tcaacgtcgg taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg
     2881 aaggttttga cgccgcaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     2941 gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3001 aatctcgtct gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc
     3061 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3121 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3181 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3241 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3301 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3361 tttagctttt accctgcagg gaattatcgt caatattcag gcggtattct ggctgcaaaa
     3421 aggcaccgaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3481 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccggc
     3541 ggcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct
     3601 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc tgatcagcgg
     3661 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3721 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc
     3781 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     3841 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     3901 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     3961 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4021 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4081 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4141 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4201 actggctcca ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt
     4261 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4321 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4381 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4441 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4501 aatacaatcc acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc
     4561 agcagcaaaa tcatgccgag gaaggcgatg caaagatagt tgccgaacgg atagagcagc
     4621 gccttaaact gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     4681 aggcagatca taatccagtt caacagcaac gttgctacca ccagcgtcat cagcagacca
     4741 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4801 agcatcagcg agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca
     4861 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     4921 accgacagcg atgccaccag aatgacgaag ttcagcgcag aagctaccac attgctgtcg
     4981 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5041 agtgccagta aaaccaccag tgaaccgata taaaacagca ggatgcgata caccacctga
     5101 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc
     5161 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag
     5221 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5281 tgaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     5341 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     5401 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5461 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     5521 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     5581 aatgaaccgg ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa
     5641 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5701 gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     5761 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     5821 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     5881 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct
     5941 ttaattccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
     6001 cagcgacagc aaaggtgcgg tgatcatgcc gccaatcatc ggggcggcaa tccggctcat
     6061 cacctctgaa ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc
     6121 caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag
     6181 cttctgctcg ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg
     6241 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc
     6301 ggtacccgtc gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac
     6361 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag
     6421 atacaacagc acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt
     6481 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
     6541 cactttttca gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg
     6601 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
     6661 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag
     6721 gatcggcaac tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt
     6781 aattggataa cgggcaatcc cttccaccct ttcgccaacc atcgccccgc ccaccgcaga
     6841 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt
     6901 aatctcaacg ttgatatagc gcccaccttc cagccgctcg gcaagcgcag aagctacgcc
     6961 tggcaccgtt cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac
     7021 agtgccggaa actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg
     7081 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
     7141 ttcctcaatg attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg
     7201 gatggtcgtt tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt
     7261 tttgccaaat acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag
     7321 catactcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
     7381 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
     7441 cagcaccgaa agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa
     7501 cagcggatga taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat
     7561 tttgccacgg atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc
     7621 acccgccatc gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc
     7681 ttccagggtg aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc
     7741 cggccccact tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt
     7801 ggcgtcagga tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac
     7861 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt
     7921 ggcattcagc ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga
     7981 aataatcgcc accagcgccg agcgcacatg ccagagaaac agcgcgcaga ctaccgccac
     8041 cacaataaac tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg
     8101 gctgcggtcg tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc
     8161 cagtttgtcc ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac
     8221 caccccgccc gccacttcgc cttcgccgtt taatgccccg gcgcatctcc gggccaatct
     8281 ggaccttcgc aacatcgcgc agataaacgg gcacgccatt ttcactggct tttaaaacga
     8341 tgtgattaaa gtcgtcgagc gtttgtagat agccgctggc gcgcaccata tattctgctt
     8401 ccgccagttc gattgacgaa ccgcccgctt cctggtttga agcatccagc gcgcttttta
     8461 cttcggcgag actgatgcca tactgcgcca ggcgctgagg atcgataacc acctgatact
     8521 ctttcaccac accgcctacc gacgccactt ccgcaacgtc aggaatggtt ttcagctcat
     8581 atttgagaaa ccagtcctgc aatgagcgta aatcggcaag atcgtgctta ccgctgcgat
     8641 ccaccagtgc atattcatag atccagccaa cacccgtggc atctggtccc agctcggcgc
     8701 tgactcccgc aggcagctta ccctgtacct ggttgaggta ctccagcacc cgcgaacgcg
     8761 cccagtacgg atcggtgcca tcttcgaaaa tgacatacac ataagagtcg ccaaactgcg
     8821 agaaaccacg cacagtcttc gcgccaggca ccgacaacat ggtggtggtt agcggataag
     8881 tcacctgatt ttcaacgatt tgcggtgcct ggccgggata gctggtttta ataatcacct
     8941 gcacatcgga gagatccggc aacgcatcca ctggcgtatt aatgatggtc caggtgcccc
     9001 agatgctcag aaacaacgcg cccatcagca ccaaaaaacg gttcgccacc gagcgacgaa
     9061 taatccattc aatcattggt tattccctca atgcgcatgg gtagcacttt cagagcgcat
     9121 ccgctccagt gcgccagaaa tattggcttc ggaatcaatc aggaacaggc cgccggaaac
     9181 caccttttca ccttccgcca gaccagagcg taatgcggtg acgccttgcg atgcctggaa
     9241 aacagcaacg cgtttcggta caaagcgccc gtcggcatca acggtaatca cccgctgttc
     9301 gttgccggta tcaatcagcg cctcgtcggc gttgtcgact tccagacgca gctgcagcgt
     9361 gcgggtcgcg gcatccacgc caggtagcag cgtccatttg cggatggtga gtgttttatc
     9421 cggtcgcgcc ggaacggtga gggtaaactg cgaggcatct ttcaccagcc aggcgatgga
     9481 ctccgggatc gcagcagtga cccacaccgg gtccataccc tgaattcgcg attacgcgca
     9541 atacccctga gaaccgccat gaacaagggt ttcatgattt gtgattttag tttagtacgc
     9601 aatttggtac acaacactat tttcacttca gcgggtagtc atcaaactca ccataacgag
     9661 catcgttgat gatgtatgtg atcaccacaa atatcacatc tggactagtt ccgtcttcat
     9721 gcgc
//
LOCUS       DAJTXY010000135         9565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTXY010000135 DAJTXY010000000
VERSION     DAJTXY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.135"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..572
                     /locus_tag="OO566_003495"
     CDS             <1..572
                     /locus_tag="OO566_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6606640.1"
                     /translation="INAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAMASQ
                     RTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGAAKMLFSH
                     AQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPD
                     FSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            590..1228
                     /locus_tag="OO566_003496"
                     /pseudo
     CDS             590..1228
                     /locus_tag="OO566_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1519..2247
                     /gene="mpaA"
                     /locus_tag="OO566_003497"
     CDS             1519..2247
                     /gene="mpaA"
                     /locus_tag="OO566_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6606641.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2222..3187)
                     /gene="ycjG"
                     /locus_tag="OO566_003498"
     CDS             complement(2222..3187)
                     /gene="ycjG"
                     /locus_tag="OO566_003498"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6606642.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3306..3812
                     /gene="tpx"
                     /locus_tag="OO566_003499"
     CDS             3306..3812
                     /gene="tpx"
                     /locus_tag="OO566_003499"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6606643.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3856..5397)
                     /gene="tyrR"
                     /locus_tag="OO566_003500"
     CDS             complement(3856..5397)
                     /gene="tyrR"
                     /locus_tag="OO566_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6606644.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5545..6606)
                     /locus_tag="OO566_003501"
     CDS             complement(5545..6606)
                     /locus_tag="OO566_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6606645.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6603..8000)
                     /locus_tag="OO566_003502"
     CDS             complement(6603..8000)
                     /locus_tag="OO566_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6606646.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8156..9154
                     /locus_tag="OO566_003503"
     CDS             8156..9154
                     /locus_tag="OO566_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6606647.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9265..>9565)
                     /gene="ompG"
                     /locus_tag="OO566_003504"
     CDS             complement(9265..>9565)
                     /gene="ompG"
                     /locus_tag="OO566_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR6606648.1"
                     /translation="SRNNGEFSTQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDD
                     IEREGHDFNRVGLFYGYDFQNGLSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         2420 a         2547 c         2298 g         2300 t
ORIGIN      
        1 ccattaatgc cgcgctgaca gaaaaacgaa ttaaagctgt agtgagtatt acgggtgtta
       61 atatcgggcg tctttttcgg gaaggtttca gcaattatga tccgattggc gtattgaatg
      121 caatggcgtc acaacgaaca aaagaggctc gtggtggaga attacagatc aacgaattac
      181 ttcccgccag cctggatgca gcaaaagccc atggactgac cgaacgcgat gtttatgaag
      241 caactgacta ctataaaaca ccacgcgggc aacaaccggg aggtgcggca aaaatgttgt
      301 tctcacatgc acagaaaacg ctggcctggg atgccttcgc atttactgaa gtgttactga
      361 cccagccggt gatggtagta gttggtgaaa aagtcggtgc cttcggtgct tatcgtgatg
      421 gactggaagt ctatggccgg gcgatggttt cgcaggatcg tcagttagtt tcgttacccg
      481 atttctcaca ttatgaacta tatgacaaac cggaagcagt tcaggaggcg ctggcaaaag
      541 ttattccttt tttcaacacc catctgggat aagtttcatg agaaatatta tgaaaaatgt
      601 actgattctt ggtgctggcg gtcagatagc ccgccatgtg attaatcaac tggcagataa
      661 acaaactatt aaacaaactt tatttgcccg tcagccagca aaaatacaca aaccctatcc
      721 aactaatagc cagattatta tgggggatgt actgaatcat gcagcactga agcaggccat
      781 gcagggacag gatattgtgt atgccaacct tacgggggaa gatttagata ttcaggcaaa
      841 ttcagtgatc gctgcgatga aagcctgtga tgttaaacgt ctgatctttg ttctttccct
      901 cggtatttat gatgaagttc caggaaaatt tgtcgaatgg aataatgcag ttattggtga
      961 acccctaaaa ccattccgca gggctgcgga tgcaattgag gcttctggac tggaatatac
     1021 aattcttcgc cctgcctagc tgactgatga agatattatt gattatgagc ttacctctcg
     1081 caatgaacca ttcaaaggta cgatcgtatc acgtaagagt gtcgcagcat taattacaga
     1141 tatcatcgat aaacctgaga aacacattgg tgagaatata ggcatcaatc aacccgggac
     1201 tgatggcgat aagccattct tcatgtaaat caattatata gataccatcg tactgtacta
     1261 tgtgcagtac gatttattaa acttattaag gtgaactgat caatcagatg cgattaacat
     1321 gtgtagaacc agtaagaata aaatacgcca tgtatataac attacgacaa ttctaatatc
     1381 tgcacatcaa tgaaaatgca aacaacttcc ggcaaaaaag tgatacaaga tcgatgacat
     1441 agcgttgaag aaaaggattt gataatccgc aaaatttgca caacactgcc tgtaatactt
     1501 cctgaaccag agacgatcat gaccgtaacc cgcccacgcg ccgaacgcgg cgcatttccg
     1561 cccggaacag aacattacgg acgttcatta ttgggcgcgc cgttaatctg gtttcctgcc
     1621 cctgcagcca gccgcgaaag tggtttgatt ctggctggca ctcacggtga tgaaaactct
     1681 tcggtcgtca cgctctcctg tgctctgcgg actttgacac cttctttgcg ccgccatcat
     1741 gtggtgctgt gtgtgaatcc tgacggctgc cagctggggt tacgggccaa tgctaatggt
     1801 gtggatttaa acagaaactt tccggcggcg aactggaagg aaggtgaaac ggtttatcgc
     1861 tggaacagcg ccgctgaaga acgtgatgtc gtattactga caggtgacaa acctggctcc
     1921 gaacctgaaa cccaggcgct gtgccagctt atacaccgca ttcaacctgc ctgggtggtc
     1981 tcattccacg atccactggc ttgtattgaa gatcccagac acagcgaatt aggcgaatgg
     2041 ctggcccagt cgtttgaatt gcctctggta accagcgtcg gttatgaaac gcctggctct
     2101 ttcggcagtt ggtgtgcaga tctcaacttg cattgcatca ccgcagagtt tccgcccatc
     2161 tcctccgacg aagccagcga aaaatatctc tttgcgatgg caaacttgct gcgctggcat
     2221 cctaaagatg caattcgccc gtcgtgaact gaagcgccgg ttccacatct accgccagcc
     2281 aggtcggtcc gtcaagatcg gcgaaactga cctgcggcac cagcggtaaa gcggcgctaa
     2341 tggcacgaga ggtacacaac atgcagccca gcatcagact gaaaccttgt gcacgcgctt
     2401 cagtcgccag cgccagcgct tccgtcagac caccggtttt atcgagctta atgttaacca
     2461 tctcatagcg ccctttcagc gccttcagat tgctacgagt atgacaactt tcatcagcac
     2521 aaatcggcaa cggatgaata aaattctcca gcgccgcatc gtcctgcgca ggaagcggtt
     2581 gttcaagcat cgcaacgcct aaatccgcca atagctggca acgcgccgcc aacccttctg
     2641 cacgccagga ttcatttgca tcaacgatca gcgtggcatc gggcacagcg gtgcgaattg
     2701 ccaccatccg ctcactgata agatggttat ccagcttcac tttcagtaat ttcgcgcctg
     2761 cctgccagag tgttgatgca ctattggcca tctgatcagg cgtaccgatg acaaccgtct
     2821 gtgcagtgat aactgtctcg ggaagcgtta tgccgatcaa atcagccagc gattgctgct
     2881 gttttcgcgc cgcgagatcc cacaatgcac aatccagcgc attacgtgct gcgccggcag
     2941 ggagaatttt ttgcaactcc tcccgtgtca gccctttctc taattgcggc acgacgctca
     3001 taatttgcgc cattaccgag gcatcacttt ctccataacg cggatacggc gtgcattcgc
     3061 cggtgccttt aataccctct tcttccagtt caaccaccac cacgcgcgct tcactgcggc
     3121 ttccccgggc aatcacaaac ggggtatgta agggccaggc ttcctcaaat accttaacga
     3181 ttctcatctt tactccttgt tgtcgcggtg gtctgcggat taagcgcaaa tagcgtttgc
     3241 tgtgttattg acagttagca taaactaggt gtgacgttaa ctatatgtaa acaggaaaga
     3301 taatcatgtc acaaaccgtt catttccagg gcaacccggt tacagtcgcc aattccatcc
     3361 cgcaggcggg tagcaaagcg cagactttta ctctcgtggc aaaagatctg tctgacgtca
     3421 ccctcggtca gtttgcgggt aaacgcaaag tgctgaacat tttcccgagt attgataccg
     3481 gtgtttgcgc cgcatcagta cgtaagttta accaactggc aactgagatc gacaacaccg
     3541 ttgtgctgtg tatctctgcc gatctgccgt tcgcccagtc tcgtttctgc ggcgcagaag
     3601 gtctgaacaa cgttatcacc ctctccactt tccgtaacgc tgaatttctg caagcctacg
     3661 gtgtggcaat tgctgatggc ctactgaaag gtctggcagc gcgtgccgtt gtggttattg
     3721 acgaaaatga caatgtgatt ttcagccagc tggtggatga aatcaccacc gagccggatt
     3781 acgaagcagc tctggctgta ctgaaagcat aagttaatat gcctgatggt gttgcaccat
     3841 caggcatatt cgcgcttact cttcgttctt cttctgactc agaccatatt cccgcaactt
     3901 attggcaatc gcggtatgtg aaacgccgag acgttttgcc agtttgcgcg tgctgggata
     3961 attgcgataa agctgggtta ataccgagcg ttcaaaacgg ctggtgattt cgtccagcga
     4021 accttccatc gcatcttcgc ccacggctac cgttgctgcg tcataatccg gcaacaaaat
     4081 atcctgtgga cgcagctcat aaccgtccag ttgtgtcagc gcgcgataga tagcgttctt
     4141 taactgccgc acatttcccg gccacgcata acgtgtaagt acagtattca ggtcagcggc
     4201 cagtttcgga cgcggcacgc cctgctcgtc ggcaaagcgg gcgacgaaca gctcagttaa
     4261 cggcataatg tcctgcggac agtcacgtag cggcggcaga ttgagcgtca acacgttcag
     4321 acgataatag agatcttcac ggaacacgcc tttttgcacc agttcgacca gattcttctg
     4381 cgtagcgcaa atcacccgca catcgacatg cacctcatgg tcttcgccaa cccgacggaa
     4441 agtgccatca ttaagaaaac gcagtaattt cgcctgcatc cgtggtgaca tttcccctat
     4501 ttcatccaac agcaccgaac caccgttcgc ctgctcaaag aaccctttct tcccttccgg
     4561 agcatgacca aacagttcac tctcgaccgc atcttctggt atagacgcgc aattcagcgc
     4621 caggtaaggt ttgcccgctc tggggcttgc ctgatggcag gcgtaggcaa aaagatcttt
     4681 acctgtacct gtgtcacccg taatcagcag cggcgcgctt agcatcgcca gtttctgcgc
     4741 ctgttcgaca acgtgcttca ttttcgggct gacggcgaca atttggctga aggcgctgac
     4801 gtcctgggcg gcgacatttt gcaactggcg gcccatacga atcgttgatc gcaacatcac
     4861 caccgcaccg gtcaggacgt gttgatcatt ttcatcctga agataaacag gcgtaatctc
     4921 catcaggaaa ttctgcccat taataacgac atgctcgtta tgcgaatctt gcggttcgct
     4981 ttccagccaa cgtaaaaaat taaagccgtt aatcaattgt gcggcggtat ggttgcgcag
     5041 gcgatccaat ttttgcccaa aaagctgaca gctcgccggg ttcgccatat ccactttgct
     5101 tttcatatcg acagagagca caggttcagg caacgcctcc agtaacgcgc tcaacgccag
     5161 atgctcacgt tcggaaggca tccacgggac agtacgcaca tcggtaacac ctgcaatacg
     5221 gcgtatttcg gccatcaaac tgctgaaact ctcaaactcc agttcagcaa aattgaggta
     5281 gattcgccca atgggatcaa tctcaatacc gcgtaaatca atgcctctta gcacgagtag
     5341 atcgagtaat tcgcgggtca gaccgagtcg gtcttcacaa aagacttcca gacgcatagg
     5401 aaccttcacc tgaaaaaaga acaattaata tgatgatatg acactatttg gatagcagga
     5461 aggtttctgt caacaatcat tgacacaaag acggaaaaac gggcacttcg gcgtaaagat
     5521 atccggtcag cacccgatat tgcattattt ttcgctgggc gttttgcctt tttgcagagt
     5581 ttctttcacc tgaccgataa gctgacgacg gaaatcccca aggcgaggtt tgtcatcgtc
     5641 aatccacggc agcgggcggc aaagctccat agctttaatc ccgagtcgtg ccgtcagaag
     5701 tccagctcca attccctgag ctgcgcgggt agacaaacga gcagcgagat cttgcgacat
     5761 ccagtccatc cccacttcgc gcaccagttc gctggctccg gcaaaagcga tattcagcaa
     5821 taccagctta aacagacgca aacggctgta ataccccagt tcaatgccat acagcgtggc
     5881 aatgcgatta attaaacgca gattgcgcca ggcgataaac gccatatcga ccaacgccag
     5941 cgggctgacc gcaatcatca acgttgattc cgccgccgaa cggctgattt cgcgccgcgc
     6001 ctgggcatct aaaactggct ggaccaaatg cgcatacagg ctgaccactt cacgatcgtt
     6061 ctgcgtttca tggattgagg cataccagcg ttgcagcgcc ggatgcgact gatcaatacc
     6121 cgcctgctgc gccagttttt cgcaaaatgc gcgacctttg cccgtgccgt ggctatgcaa
     6181 taaatcacgc gcttcgtcgc gttcatgggc gcgctgtcgc aagcgccata agcgccgcca
     6241 ctctgttacc acagaaccta cgccagcgcc gataatcaat gccccagcgg cacatccacc
     6301 cagcgctacc cagtcctggg tttgccaggc attcattgtc cactgtatac cctgcccgac
     6361 aacgcttgcg ccaaacagag ccagcccgcc catcaccatt ttgcgccaca ggctgcgttt
     6421 cggacgtaac gctgcgtcca ttaccgcttc gacttgcccc tcttcttcct gcgcttcgtc
     6481 gagcgtggcc ggggcaaaat tttgcgcctg attttcgtca aaggtctgct gcgccctgaa
     6541 ttttggattc tgttcgacct ccagcggacc gtcgaaatca atacgtggtt ttaacggttc
     6601 ggtcatcgca atttatctcc tattaaaaat tccagcgcgg catcaagacg aatatgcggc
     6661 agcggtttgt cgacatccat cacctgcggg cgaaacgctt caaactggaa cccttgcttg
     6721 tcccagaacg cctgaccggg caaacgcgca ggaacttcac cggggtaaac ggtgagaggc
     6781 gcaccatcgc tgagccggtt accgcgcaat gccggaattt tctcgccgtt gacgtcaata
     6841 atgccgctgg tggtcgtctg caccgaggcc agccccaggc aatccatact aatcctttca
     6901 aacgccgcat tttgccaggc atcctgaatc agttgttgca gtaatgaaac catattggcg
     6961 tgctgatcga ccgttacatg gtccgctttc gtcgcggcaa acagcagttt gtcgataacc
     7021 ggcgagaata agcggcgaaa cagcgtgcgc tgcccgtagt ggaaactttg catcagctgc
     7081 gtcagtgcca gacgcatatc attaaatgcc tgtggcccac tgttgagagg ttgcaggcaa
     7141 tccaccagca caatctggcg gtcaaagcgc agaaaatgat tcttatagaa ccccttcacc
     7201 actttctcgc agtaataatt aaaccgctcg cgcagcattc cggcattggt gtgcttatcg
     7261 gcctgcgcca gtttggactc gccccaggca tcgacatccg gccacgggaa gaattgcagc
     7321 gcgggcgcac ctgccatatc tcccggcaag acaaagcgcc caggctgaat aaagtgcagc
     7381 ccctgctgtt tacagtggtg gagataatcg gtccacacgg cggcaatgtc tgccagccgg
     7441 ttttcgtcgg caggcgctag cgggtcaagt ccttcgcaca tcattcgcca tttcgccgac
     7501 cattcgccgc gctgaccatt gagtaagccc gtcatctggc gcgaccagct taagtaatcc
     7561 tgcgccagca tcggcaggtc gagcaaccat tcgccgggat aatccacaat ttccagatac
     7621 agcgtggagg tatccttaaa gtggcgcagc agcgaatcgt tcgatttaaa acgtaacgcc
     7681 aggcgaattt cactgacgcc gcgcgtaggc gtcggccagg cgggaggatc accatataac
     7741 tgcgccagtc cttcatcata ggtgaagcgc ggaatgccaa agtcacgctg gggaatgcgt
     7801 tttacgccca gcaagcgctc ttcacgcacc gcgcttaaca gcggcaaacg tgctccggca
     7861 tgaatattga gcaactgatt aaccatcgcg gtgataaacg ctgttttgcc gctgcggcta
     7921 agtccggtta cagcgaggcg cagatgtctg tcgacacccc gattcaccag cgcattgagt
     7981 tcatttttaa gtcgcttcat tgctgtcctg tgctgctctg aagaccagaa aaaaaatcat
     8041 tcgtaaggat atgaatgtgg gggccaggcc attgattatc aactgcccgt gttgtcatcg
     8101 acgggcgaac tccataagat gtgcagcatt cacgttcgga cggagttcct gacctatgtc
     8161 ccctactatt tatgatattg ccagggttgc aggcgtatca aaatccaccg tatcacgcgt
     8221 gctgaataag caaactaata tctccccgga agcgcgcgaa aaagtgttac gggccattga
     8281 agaattacag tatcagccaa acaagctggc ccgcgcgctg acctcttcgg gttttgatgc
     8341 cattatggtg atttctaccc gttcgaccaa aactacggcg ggtaatccgt ttttctctga
     8401 agttttgcat gccatcaccg ccaaagcgga agaagaaggt ttcgacgtga tattacaaac
     8461 gtcgcacaat ccggcagaag atctgcaaaa atgcgaaagc aaaattaaac agaaaatgat
     8521 taaaggcatt attatgctca gttcgccagc ggatgagtca ttttttgccc aactcgataa
     8581 atacgatatt ccggtggtgg taattggcaa agttgaaggt caatatgccc atgtttattc
     8641 tgtcgatacc gataattttg gtgacagcat tgcgttgacc gatgcattaa ttgaaagtgg
     8701 gcatcaaaat attgcctgcc tgcatgctcc gcttgatgtc catgtttcgg ttgatcgcgt
     8761 taatggctat aagaaaagtc tgtccgcgca taatattgca gtgcgtgata aatggattgt
     8821 tgacggcggt tatacccatg aaacagcact gcaagcagca cggcaattat taagtcagtc
     8881 accgttgcct gaggcagtgt ttgccactga cagcctgaaa ttaatgagca tttatcgtgc
     8941 ggcggcagag aaaaatattg ctattccgca gcagttagcg gtggtgggtt atagtaatga
     9001 aacgctgtcg tttattttaa cgcctgcacc aggcggcatc gatgttccga cgcaggagtt
     9061 aggtcaacaa agctgcgagt tattattccg cttaatttcg ggaaaaccgt caccacagaa
     9121 tattaccgtt gccacgcata tgacgctgaa ataaatacca gctaccttgc ctgaatgccg
     9181 gatgcgatgc tggcgcatct tatccggcct acatttatag atcggataaa gtactcgcgc
     9241 cgcatccggc aatttagccc attatcagaa cgagtaattt acgccgacac ctgcataatg
     9301 gaatttatcg ctgtcgcctt cgtcgtgatc ctgccactca aacgcgtatt ccagcgaaac
     9361 ggaaagtccg ttctggaaat cataaccgta aaataaaccc acacggttaa aatcatggcc
     9421 ttcacgttca atatcatcct gccagtccca gttactccag cgatccagcc caatgcgcgt
     9481 atacggcgta accgagtggt tgccgagcgt cagcggcaaa taggcgcgaa tttcttgtgt
     9541 ggaaaactca ccgttattgc ggctg
//
LOCUS       DAJTXY010000136         9460 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTXY010000136 DAJTXY010000000
VERSION     DAJTXY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.136"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..360)
                     /gene="ompG"
                     /locus_tag="OO566_003505"
     CDS             complement(<1..360)
                     /gene="ompG"
                     /locus_tag="OO566_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR6606649.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYV"
     gene            complement(405..1487)
                     /locus_tag="OO566_003506"
     CDS             complement(405..1487)
                     /locus_tag="OO566_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6606650.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(1501..2160)
                     /gene="pgmB"
                     /locus_tag="OO566_003507"
     CDS             complement(1501..2160)
                     /gene="pgmB"
                     /locus_tag="OO566_003507"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6606651.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(2157..4424)
                     /gene="ycjT"
                     /locus_tag="OO566_003508"
     CDS             complement(2157..4424)
                     /gene="ycjT"
                     /locus_tag="OO566_003508"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6606652.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     ARGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(4421..5027)
                     /locus_tag="OO566_003509"
                     /pseudo
     CDS             complement(4421..5027)
                     /locus_tag="OO566_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(5075..5821)
                     /locus_tag="OO566_003510"
                     /pseudo
     CDS             complement(5075..5821)
                     /locus_tag="OO566_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
     gene            complement(5852..6694)
                     /locus_tag="OO566_003511"
     CDS             complement(5852..6694)
                     /locus_tag="OO566_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR6606653.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVTWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(6681..7562)
                     /locus_tag="OO566_003512"
     CDS             complement(6681..7562)
                     /locus_tag="OO566_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6606654.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLLAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7583..8866)
                     /locus_tag="OO566_003513"
                     /pseudo
     CDS             complement(7583..8866)
                     /locus_tag="OO566_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(8880..>9460)
                     /gene="ycjM"
                     /locus_tag="OO566_003514"
     CDS             complement(8880..>9460)
                     /gene="ycjM"
                     /locus_tag="OO566_003514"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR6606655.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMDALSRRESSDEERCARFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         2263 a         2633 c         2315 g         2249 t
ORIGIN      
        1 aacgtagtga taaccataat tacggaaacc gccggtcagg ccgaaactga aatcatcgct
       61 ttcgaggaac tgataatgca cctccagctc cgggcgatca aaccacgttc cacgtttacc
      121 cgcgctataa tctaccggcc cttcctgata ataggccaga gcaattctcc acggcccgtt
      181 ggcggcatta aaatagactg aaggctccgc cagcccatcc atatcttcgc cataaccctc
      241 gacgttttct atttcgtaca tcgcaccgat attaaagtgc cagtcgttct tttcctcggc
      301 ctgtgcgcag gccattcccg cacacatcac cagtgcggta cagggtaata actttttcat
      361 tattgttcct tgtattatcc taggggaatg ccttccccct gtatttagcg aattgctatt
      421 tccgtttctg catcaaagaa atgacatttc gtcatatcaa aatgaatagt gatattttct
      481 cctgcatgat aatcatttaa cgcaccagca cggaccacta actcgtgccc cccaaccgtg
      541 gtgtagagca taaattccgc accggttaat tctgccacgc taattttggc ggaaatgtta
      601 ttttcctctt gcgcgtccgg atgaatatct tccgggcgta ttcccatcac gatgggctta
      661 tgcaaacttt cctgtgtttt cagaaccgct aatttctctt cgagaatggt taatttaagc
      721 gtttccgtaa cgaatttatc gccatcgatc gtgccgcgaa taaaattcat cgctggtgat
      781 ccaataaatc cggcaacaaa catattcgcg ggttggttat aaacggtttt cggcgcacct
      841 acttgctgaa caatcccgtc tttcataatc acaatccgcg tcgccatggt catcgcttcg
      901 gtctgatcgt gggtcacgta gatcatggtg gtgttcagtt tctgatgcag cttgctgatc
      961 tctgcacgca tttgtacgcg cagcttggca tcaaggttag agagcggttc atccattaaa
     1021 aacacgcccg cttcgcgcac aatcgcccgc ccgagcgcca cacgctggcg ttgcccgccg
     1081 gaaagcgcgc ccggcttgcg tttcaggtac tcacgcaggc cgagaatttg cgccgcccag
     1141 ttcacccgct catcaatcac ctctttggcg attttttgca tcttcaggcc aaacgccatg
     1201 ttgtcgtaaa ccgtcatatg cggatacaac gcgtagttct ggaacaccat cgcgatattg
     1261 cgtgctttgg ctggaacgtc attcattcgt ttgccgtcga tcaacagatc gccgccgctg
     1321 atctcctcaa gcccggcaat catgcgcagg gtggtcgatt taccgcagcc cgacgggccg
     1381 acaaatacga tgaactcttt atcggcaatt tccaggttga agtccttcac cacatgcacc
     1441 tggttatcgt agattttttg aatatgttgt aacgaaagct gagccatgtt gattcctttg
     1501 ctatacgttt tgccagaagg ccgataaccg cgaccaggtc agggagtccg ttgagggcaa
     1561 cagtaattgc gccccggtta agcccgcacc gatccccacc gagcgcatac cgctggcgtt
     1621 aatggcgtca atgcccgcct gcgcatcttc aatgccgata catgcctgcg gcggcacgcc
     1681 cagcccggca caggcagcga gaaagatttc cggatccggt ttcgagtttt taagttggga
     1741 agcatccgcg cagaaggtga aaaactcgcg cagctccagc gccgctaaaa tcgtcggcgc
     1801 attcagggag acggaagcca gccctaccgg aatctgctgt gcacggagct ctgccagcaa
     1861 attgcgaata ccgggcagca cagcattaac cgtcagctcg cgcagtgagt ggacgtaaag
     1921 cagatttttg cgatacgcca gttgcgcccg ctcctgcggg ttaaagtcgc cctctttgcc
     1981 cccgtgttgc agaatgcgcc gcagagactc atcgcggctg atccctttta gagattcgtt
     2041 aaactgcgca tcaatactga tgccaatttc agcggcaatc tgctgccacg cctggaaatg
     2101 cagatgcgcg gtatcggtga ttacaccatc cagatcgaaa attacccctt gcagtttcat
     2161 tcatcctcct gatgcgtggt agcggtccca ttgaagggca aaataaaatc acccaaacag
     2221 aaaacagatt cttcagccac ggatataagc tgaccgttca aacgcagtga aacgggcgca
     2281 gaagttcgaa tcgcaatacg ctgcgcgtcg agagtgacct gtaattcgca gccctgccag
     2341 aacagaggga aagacaactg ttgccactgc tcaggtaacg ccggatttag atgcaattca
     2401 ccgtcacgca cgctcacccc ggcaaaaccc tgaatcgccc ccagccagat agcgccagtt
     2461 gcggcagcat ggataccatc atcacaacta tgcggatcag caccaagatc gatttcagtc
     2521 ccctcgcgcc agaactgata actttgggtc agcaggccac agcgtgcagc aacaatgccg
     2581 tggattgctt tacttaatga cgagtcgtga atagtgcgcg gttcataaaa ttgcagattg
     2641 gcaagacacg atgccgatga gaactgctct ggcagcatgt aattgagcat caccacatca
     2701 gcttgtttga ggatctgcat ctcgttcact tctgcgcgtg aataatccag cagaatggtt
     2761 tgcttccccg ccgccgcttt gtatttcgcc agattaatcg ccggcttagt cataaacgaa
     2821 tcatcctgcg gcaaaacacc gtcgggctga atttctggca gccgcagttc tttaaggaac
     2881 atttcggcac gatgaataaa cgcgtcgtca ctacagccaa actggcgggc aatactcagc
     2941 gcctgctgga cgttgtaata caccatatag ctggtgaagg cgttattatt gacatgttcg
     3001 gtatattcgt ctggcccaat aacatcatga atttccagac ggtcgttaac cctcaccgcg
     3061 cggctaatcc agaactttgc agtttccaga agtagcgcca tgccttcatg cgcaatgaaa
     3121 ctttcatccc ccgtggtctg ccagtattga ataaccgccc aggcgatatc ggccaccaga
     3181 tgatgttccg cctgcgccga ggccactttt tgccgcagcc cggtgcgaat gttaatggcg
     3241 gcaaattccg gcgtctcttc ttcgccgctg cgcgcgcttt cccacggaaa tagcgcgccc
     3301 tgccagccgt tgcgtcgcgc tttctcctgc gcgcctggca agttgtgcca gcgataacgc
     3361 agtaaacttc gggcaaccgt cggatcgcta aacagatgga acggcagcaa aaatacttct
     3421 gtatcccaga aaacgtggcc tttgtagcct tcgccggtta agccttttgc cgcaatactg
     3481 ctgcgctcgc cgtgagccgg cgtcatgatg cgcagatgat aaagcgcata atctaacgct
     3541 tgctgatcgt gcgcatcgcc gccatttacc gtgatacgac gtttctgcca ccattgacgc
     3601 cagttttctg ttgatgctgc aagatgttgg tcgtaactct gttgcgcgca catttcaagc
     3661 tggcgaagcg acacgctgcc ccactcgtct aaagcagctt gcctgtcatc ccgccagtcg
     3721 atccacacca gtttttgcaa cgtcactgtc tcgcctgcat gaagctgcgc actggtatgt
     3781 tgcagtaaac ggcgctcttt ggcggtatag cattgctgca catcaccgct cacctggcag
     3841 caacaactga tggccacatc actgcgccca tcctgggtgg tgtagatccc ctgcatcaaa
     3901 tgctgaccaa acacccgcac ctgggtttcg tcgagatgct ggcgaccgtg gttggtttgc
     3961 gtggcgtcaa tgcctgttga aatcagcact gacgcgtcgg cgtccagtgg cataatagtg
     4021 atttccagcg caatgagcgg cagttggtct gccgaaacaa agcgacggct ggcgatggtg
     4081 taacctgcgc cgttgctggt acgccagaca acgctacggc gtaattctcc actggcaaag
     4141 tcaagttcac gctgccaggc ttcgcgggat aacgagaaaa cctcaccatt tatggcaatc
     4201 tccatcccca agatatcagg cagattcacc agttcgttga tttcaccttt tcccgcccga
     4261 tgatacagcc ccgccagata catccctcgc gcctggcgcg tgtaatcttc ttcatggctg
     4321 gcgcgaagcc caagatagcc gttcccctgc gccatcagcg atgcatactt gttcagggta
     4381 tgctggctga aatggggttc tgataacgtt actggcctgg tcataattcc acacgcgtcc
     4441 ctgtttctgc ggattgatat aacgccgcca ccagttgctg gatgatgtac ccctgctcgg
     4501 cgtcggctat catcacgggc ttgccctgta cgtgattgat aaaggcttcc atgctgcgca
     4561 aatggcggtt gtcgtctgcc atttcccgtt gcatcagcgt cattaattca ccgttgttat
     4621 cggtgtagat atgtgctgga aacagcgtcg caccggcttt atcaccacaa aagctgacgt
     4681 tcataatcaa ctgttcgcgg atgttgagtg caaatgacgt ttccagccac agaatgccgc
     4741 cgttatgaaa ttcaatggtg ccaaacagcg aatcttcgac gctgtaagtt gccggatccc
     4801 actcgccaaa ttgaccacag ctcttttgcg tgccgatctt ttgaaaacta tgcgcattca
     4861 cgcttttcac cgccggaaag cccagcacat acatcgcagc atccagcata tgaatgccga
     4921 tgtcgatcca gttctttatt ggtaaagaca ccccagccgg gaacgccgca gcgacgcagg
     4981 gcgcgggcgg tggtaacgta aatctctccc aaaacgccgt tggtcacctg ttcacgcagc
     5041 tgttgcgtat cgagcgcaaa cgatggtgaa agtcgtaggc caggccgcgc agcgccgatt
     5101 gcagcgcgtc ggcgtaaccg ctggtttcga taatcacatc ggccccctgc ttgccggtca
     5161 gcgttttgat ctctttgcct acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt
     5221 ggcgacgggc aatatcacag cgatgggcga taggatcgac gccaattacc accgatgcgc
     5281 cagcgcgttt cgccagttgg atggcaattt gaccgatcgc gccaagccct accaccacga
     5341 caaaatcccc tacgcgtacg ttggcatcgc gcacgccgct catggcaaac tgcgccgggt
     5401 cgtaacagac ggcgtttttc caggagctgc cttgcggcat tttgcgcagc ttgtagttat
     5461 tcactgcgtt aatgatgacc gtctcggaga gcgggccgta gccgcatacg ctgtcgccca
     5521 ccgcgtagtc tgtaacgtcg ctgccgcact cgataatgtc gccaaccacc atgttgccaa
     5581 gctggaattt gccaaactca atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct
     5641 gccactcgcc gttaaagtct tcatcgataa acgggctggc ggcgcggaag tcgaccactt
     5701 ccgttccgtg tttcggtgcg ccgaaacgca cgcggatctt cacttcattg gctaaaatcg
     5761 cccgatcttc atactcaacc agcgcagcaa cacgcggtgc tgtggctact aactttttca
     5821 tgtaaaactc cttgtaaaga aactggccgc tttagccctt cacgccaccg gcggtcaaac
     5881 cactcttaat aaaacgttcc gaaagcgcat acataatcac caccggcaat gcggtcacca
     5941 gcgaggccgc catcatccgt ccccagatgt aatcaggcgt actgaacagc gcgttcaggc
     6001 ccactggtaa ggtgaaattg ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt
     6061 tccacgtcac cataaagcag tagacgaaca ccgatatcag cccggacatc gccaggggca
     6121 cggtgatacg gaagatgatt tgcagacggt tgaggccatc catcatcgcc gcttcttcga
     6181 tctcatcagg gatggtgtcg aagtaacttt tcagcataaa cacggcggtg ggcagggttt
     6241 gcgtcaccat ggtaatgatc agcgccatct cggtgtcgta aataccaagc gcggtgatga
     6301 ttttgaacag aggcaccacc agcaaaatgc cagagaacat atacaccgtg taaaagctgg
     6361 cgttgatggt catccggcct ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc
     6421 cgagaaatac cgccaccacg gaggaaacca ccgacaccac catgctgtta cggaagtagt
     6481 cgacaaacgg gaaaatcatc gggttaaaaa tgtcgacgta atgctccagc gtccactgtt
     6541 gcggcagcag cgtaggatgc agcgatatcg cctctttcgc gcttttgaac gaggtcatca
     6601 gcatcacaaa aaacggaaac agcgtgatga tgagaaacag cgccagcccg cagtaaaaac
     6661 cgatacgact gagcgtgcgt ttatttgttg ccattgaggt tcacccgttt cctggtcagc
     6721 agaatgacag cgaaaataat gatgaagagc accaccgaga tcgccgccgc tttccctaag
     6781 tcattaaagg caaaggcggt tttgtagaga tagacaccaa gaatatcgac cttggttgtc
     6841 agcagataaa catcagcgaa catgtagaac atccagatgg tgcgcagggt caccaccgtc
     6901 gccaagaccg gcataattgc gggcagcgtg acgatgcgaa accgctgcca ggcattagcg
     6961 ccatccatct ctgccgcttc gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac
     7021 gagataaagg catacgggaa gtagcgccag atggcgagca gcaccaccag cacaaagcta
     7081 ctgcccggat tatcaaacca cagcggtgcc tgctcataga gatgcagaag atcgacgccg
     7141 aggtagttca caatgccgta gccgttgttg aacatgtatt tccaggcaaa caccagcgaa
     7201 atggacggcg ttacgtagga gaggatcacc agcgaacgcg cggttttgcg caggcggaac
     7261 tcgcggttga aaaacatcgc cacggccagc ccgagcacgg tgctgcccgc caccaccagc
     7321 gccgtatacc agaccgtcat ccacagcgag tgccagaagc cgggatcgga gagaatgcgc
     7381 acatagttac tcaccccaac aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa
     7441 aaactgattt cgatattcga caccatcggc cacgccacca gaccgcccag cagtaataag
     7501 ctgggcgcga gaagcagcag cgcaaacggc atatcggaac gacctgaaaa aagcctgttc
     7561 attgcggatt cctgtcctgg gtttagtgct gttcgatcag ttcatccagc tttttctggc
     7621 tcgcttgcag cgtgccaggg agatcggctt tacccacggt gacgttatgc accatggagc
     7681 tcaccacacc agaacccgtc acatcgccca tgcgggtaaa gtttttatcc cctactgcgc
     7741 caaagacctg aatatttggc agttcaccga ttagctgatt cggtagttca cccagcgcct
     7801 taataacgtc gttgtctttc caagtggcgg tagtgacaac cgctttattc accggcagcg
     7861 cagcacctgg cgacatcatc acccagtcgg caatgttgtc cgcctgctcc ataaaggtga
     7921 caaatttttc tgctgcttcg gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc
     7981 cgtagaccgc agagtttttc tcggttggca ccacgaaacc gacgtttttc gggtcgcctt
     8041 ctttaatcac agccggaagg atataggtgg agtaaatcgc catcggcgcg gtgccgttca
     8101 taaaggcgtc tttcacttcc atgatgtcgt tagaacccgg catggtgttg gcagcaaggt
     8161 cgcggtaata ggtcagcgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg
     8221 cgttaaagac gttggcctag ttggataacg caaactggga gaaggattgt tccgtcagca
     8281 cgctttctgc cgtaggcagc gcaataccgt attttttatt cgccgggtca ttcagtttct
     8341 gtgcaacgtc cagcagctgt tgccagtttt tcggctcctc cagccccgct tttgccagca
     8401 catctttgcg ataccagatg ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc
     8461 taccatcttc ggtacgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt
     8521 tgctgatgac cgtggcaacc gctttgcgat cgataagctg ctctttatcc ataactttgg
     8581 cgtagtcatg gctggtttcg atcacttccg gcagcgagcc gctacgtgaa agggtgatga
     8641 ctttggtgtt ataagcgtct tcttccacgg gcacttgttt aacggtgata cctgggtttt
     8701 ctttttcaaa acgggcaatc aatttgctga taactgcctg acgctcctgc tccaccgaag
     8761 aatgcataaa ttcgatggat acatgggttt tattttcttc tttacagcct gaaatcaggg
     8821 cgcaggaaac cagtgctgat aacagcacga tttttgattt aatcatgaga tgttcctttt
     8881 tatttaatcc acataacctg ccaagggcgc agcgttattt cattacccaa tatatcaact
     8941 tcactaatca gatcccgacc atgcagatca gtaatattta catgctgaat atttttactg
     9001 acattaaaca atccagtcag acaattacca tcagcgttac ttctttgaat acgcattacg
     9061 gatgaattaa ccgtatcgat ggtaaaatta ttatccggat gaaactcgtt atggctgcga
     9121 cgaagtgtaa ttaaacgcga caactcatga tataccgcat gccttaatgt agcttcatcg
     9181 ttcagttctc gtgttatctc ttcactgtga tattttttac ggttaatcgc acggttatat
     9241 ccgagttttt cgacacctgc gtaatcatta cgcgagccaa gaatactttg aatatatatc
     9301 gcgggtacac cggggaaact taacaaaatc gcatgggcaa ggataaacct ggcgcaacgt
     9361 tcttcatcgc tactctcgcg gcggcttaac gcatccatat aggtgacatt catctcatag
     9421 ggactgcgcg tgccgtcggg attatttttc cagtttacta
//
LOCUS       DAJTXY010000137         9425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTXY010000137 DAJTXY010000000
VERSION     DAJTXY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.137"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(397..603)
                     /locus_tag="OO566_003515"
     CDS             complement(397..603)
                     /locus_tag="OO566_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6606656.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            916..1005
                     /gene="kdpF"
                     /locus_tag="OO566_003516"
     CDS             916..1005
                     /gene="kdpF"
                     /locus_tag="OO566_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6606657.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1005..2678
                     /gene="kdpA"
                     /locus_tag="OO566_003517"
                     /pseudo
     CDS             1005..2678
                     /gene="kdpA"
                     /locus_tag="OO566_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            2701..4748
                     /gene="kdpB"
                     /locus_tag="OO566_003518"
                     /pseudo
     CDS             2701..4748
                     /gene="kdpB"
                     /locus_tag="OO566_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4757..5329
                     /gene="kdpC"
                     /locus_tag="OO566_003519"
     CDS             4757..5329
                     /gene="kdpC"
                     /locus_tag="OO566_003519"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6606658.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAVAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5322..6305
                     /locus_tag="OO566_003520"
                     /pseudo
     CDS             5322..6305
                     /locus_tag="OO566_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            6305..8001
                     /locus_tag="OO566_003521"
                     /pseudo
     CDS             6305..8001
                     /locus_tag="OO566_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
     gene            7998..8675
                     /gene="kdpE"
                     /locus_tag="OO566_003522"
     CDS             7998..8675
                     /gene="kdpE"
                     /locus_tag="OO566_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR6606659.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8950..9054
                     /locus_tag="OO566_003523"
     CDS             8950..9054
                     /locus_tag="OO566_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6606660.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
BASE COUNT         2083 a         2382 c         2621 g         2339 t
ORIGIN      
        1 tattcttaat atatcaataa agtatatatt actctcttta ctatctgttt ttattgcggt
       61 agcgatacta ataacaaaat tcggaattta tttagatgct ggaattagaa aaagggatga
      121 cggctttctc accttaccct gaagtttcac gaaagtgtgt aaggaagtat gcaatctaac
      181 tggagatttc tgatgatcta gtcgcttaga gcgacaatga aggtgaccgc atcctgggta
      241 gccctatgaa aggatactat tgaaacgtga tgctaaaaca gtgatgaaat gaataatacg
      301 atggaaacgt cttccatgtt aattatcatg gaagacgttg atgttgaaca cctaaaccag
      361 ccccctgata cgaggctggc ttaagcgcgc aggcaatcag taaaaatcac cggttgcctt
      421 ttcggcctga tccatccaca ccggtttatc gctggttttc gaccagacgc ggtgcaggta
      481 gctgtaaaag cgggcgcggt ctttccagaa aaacatgaaa ggcagcgcca gaacgcccgc
      541 tgcaaccgca tatgtacggc gtaaaaagat aagccatgca ggatattctc tgtagagttc
      601 catacttttc tccccctttt gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt
      661 tatctgatga aaatagtacc gccttttgtg taattttacc actcatccga ccacttattt
      721 ttgcttattg atggtttatt tacattcaca ctgtaattaa attacacaaa agttaaatta
      781 atactaacca tcagttaaat catggccttt gccattttta tacttttttt acaccccgtc
      841 cgcagatttt tgcgaaatct ttgcagccag aattctactc ttccggtatc acttttaggc
      901 tactggaggt gcactgtgag tgcaggcgtg atagccggcg tgttgctggt gtttttatta
      961 ctgggttatt tggtttatgc cctgatcaat gcggaggcgt tctgatggct gcgcaagggt
     1021 tcttactgat tgccacgttt ttactggtat taatggtgct ggcgcgtcct ttaggcagcg
     1081 ggctggcgcg gctgattaat gacattcctc ttccaggtac agcgggcgtt gaacgtatac
     1141 tctttcgttt acctggcgtc tctgaccatg agatgaactg gaagcaatat ctttgtgcca
     1201 ttctcggcct gaacatgctg gggctggcgg tgctgttttt tatgttgctc ggtcagcact
     1261 atctgccgct taatccatag cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg
     1321 ccgtcagctt tgtcacgaat accaactggc aatcttatag cggtgaaacc acgttgagct
     1381 atttcagcca gatggcaggc ttaacggtac aaaactttct ttctgccgcc agcgggattg
     1441 cggtgatttt tgccttcatc cgtgcgttta ctcgccagag catgagcaca ctcgggaatg
     1501 cctgggtcga tctgctacgc atcacgttat gggtgctagt ccctgtggcg ttgttaatcg
     1561 cactgttttt tattcaacaa ggtgcgctgc aaaactttct gccctatcag gcagtgaata
     1621 ccgttgaagg agcgcaacag ctgttaccca tgggaccagt agcttctcag gaagcgatca
     1681 agatgctcgg tactaacggc ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa
     1741 acccaaccgc actgaccaac ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc
     1801 tgtgctttgc ctttggtgaa gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg
     1861 cgatgtcagt gatttttgtc atctgcgtag gcgtggttat gtgggcagaa gttcagggta
     1921 atcctcatct gctggcgctc ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc
     1981 gtttcggcgt gctggtcagt agcctgtttg cggtcgtgac tacggcggcg tcctgtggcg
     2041 cggtgattgc gatgcatgat tcgtttaccg ctctcggcgg catggtgccg atgtggctaa
     2101 tgcaaattgg tgaagtggtg ttcggcggtg tcggttctgg tctttacggc atgatgctgt
     2161 ttgtcctgct ggcggtgttt attgccgggc tgatgattgg tcgtacaccg gaatatctgg
     2221 gtaaaaaaat cgacgtacgc gagatgaaac tgactgcact ggcgattctg gttactccaa
     2281 cactggtact gatgggcgcg gcgttggcga tgatgaccga cgccggacgt agcgccatac
     2341 ttaaccctag cccgcatggt tttagcgaag tgctgtacgc cgtgtcgtcc gccgctaaca
     2401 acaacggcag cgcctttgcc ggattaagcg ccaactctcc gttctggaac tgtttactgg
     2461 cgttctgcat gtttgtcggt cgctccgggg tgattatccc ggtgatggca attgccggtt
     2521 cgctggtgag taaaaagagc caacccgcca gctccggcac gctgccaacg cacggcccgc
     2581 tgtttgttgg cctgttaatc ggcaccgtgt tgctggttgg cgcactgacc tttatccctg
     2641 ccctggcgct tggtccggtg gcggaatatc tctcctgatg atattgagtg agcactgaat
     2701 atgagtcgta aacaactggc gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa
     2761 gcggtgaaaa aattaaaccc gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg
     2821 atcggcagtc tgctgaccac ctgtattagc atcgcgatgg caagcggtgc gatgcctggc
     2881 aatgcgctgt ttagcgcggc aattagcggc tggctgtggg tcaccgtact gttcgctaat
     2941 ttcgccgagg cgctggcaga aggccgcagt aaagcgcagg ccaacagtct taaaggggtg
     3001 aaaaaaactg cctttgcccg caagctgcgt gagccgaaat atggcgctgc ggcggacaaa
     3061 gttcctgccg atcaactgcg taaaggtgac atcgtgctgg tagaagctgg cgatattatc
     3121 ccctgcgatg gtgaagtcat tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg
     3181 gaatcggcac cggtgatccg tgaatccggc ggcgattttg cctccgtcac cggcggcacg
     3241 cgtattcttt ctgactggct ggtgattgag tgtagcgtta accccggtga gacatttctg
     3301 gatcggatga tcgcgatggt ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc
     3361 ctgactatcc tgctgattgc tctgaccatc gtctttttac tggcaaccgc cacgctgtgg
     3421 ccgttttccg cgtggggcgg taatgccgtg agtgtaacgg tactggtggc gctgctggtc
     3481 tgtctgatcc caaccactat tggtggcctg ttgtcagcga tcggcgtcgc cgggatgagt
     3541 cggatgctgg gcgcgaatgt gattgccacc agcggacgtg cagttgaagc ggcaggtgac
     3601 gttgacgttc tgctactgga taaaaccggc accatcacac tcggtaaccg tcaggcgtcg
     3661 gagtttatcc ccgcgcaggg cgtggatgaa aaaacgctgg ctgacgccgc acaactggct
     3721 tcgctggctg atgaaacgcc ggaaggtcgc agtattgtga tcctcgccaa gcaacgtttt
     3781 aacctgcgcg agcgcgatgt gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa
     3841 agccggatga gcgggatcaa catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc
     3901 attcgccgcc atattgaggc taacggcggt cacttccctg ccgatgttga tcaaaaagtc
     3961 gatcaggttg cgcgtcaagg agccacgccg ctggtggtgg tggaaggttc tcgtgtgctg
     4021 ggcgttattg cgctgaaaga tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg
     4081 cgcaaatggg cattaaaacg gtgatgatta ccggcgataa ccgtctgact gccgccgcga
     4141 tagctgcgga agcgggtgtc gatgattttc tcgccgaagc gacaccggag gccaagctgg
     4201 cattgatccg tcagtatcag gcggaaggtc gtttggtagc gatgaccggc gacagcacca
     4261 acgatgctcc ggcgctggcg caggcagatg tcgcggtggc gatgaactcc ggcacccagg
     4321 cggcgaaata ggcgggcaat atggtggatc tcgactctaa cccgaccaag ttgatcgagg
     4381 tggtgcacat tggcaaacag atgctgatga cccgtggctc gctgaccacc ttcagcattg
     4441 ccaacgatgt ggcgaaatac ttcaccatta ttccggcggc gttcgcggca acatatccgc
     4501 agttaaatgc gctgaacatc atgcgcctgc attcgcccga ctccgccatt ctcagtgcgg
     4561 tcattttcaa cgccttgatt atcgtctttt tgattcctct ggcgttaaaa ggcgtgagtt
     4621 ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac ggtctgggtg
     4681 ggctgctggt gccgtttatt ggtatcaaag tcattgattt actgctaacc gtttgtagtc
     4741 tggtgtgagg tttaccatga gtggattacg tccggcatta tcgacatttc tctttctgtt
     4801 attgattact ggcggcgttt acccgctgct gaccaccgca ctggggcaat ggtggtatcc
     4861 ctggcaggcc aacggttcgt tgattcgtga aggtgatacg gtgcgcggtt cggcattaat
     4921 cgggcaaaat tttaccggca acggctattt tcatggtcgc ccgtcggcaa cagcagaaat
     4981 gccctataat ccacaggctt ctggcgggag caatctggca gtcagtaacc cggagctgga
     5041 taaacagata gccgcacgcg ttgccgcatt acgggccgct aacccggacg ccagcacgaa
     5101 cgttccggtt gaactggtga cggcgtcggc aagcggactg gacaataaca tcaccccgca
     5161 agcggtggcc tggcagatcc cacgcatcgc aaaagcgcgt aatctcagcg ttgaacagct
     5221 cacgcagctg atcgcaaaat acagccaaca accgctggtg aaatatatcg gccagccggt
     5281 cgtcaacatt gtcgaactca atctggcact ggataagctt gatgaataac gaacccttac
     5341 gtcccgaccc cgaccgtctg ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag
     5401 ttttcttcag tgcctgtgca ggcgtcggga agacctgggc gatgctggca gaagcccagc
     5461 gactgcgggc gcaagggctg gatattgtgg ttggtgtggt agaaacccat gggcggaaag
     5521 ataccgccgc catgcttgaa gggctggcta ttctgcctct gaaacgccag gcgtaccgtg
     5581 ggcggcatat cagcgagttt gatctcgatg ccgccctcgc ccgccgtccg gcgctgatct
     5641 taatggacga actggcgcac agtaatgcgc caggttctcg tcatcctaaa cgctggcagg
     5701 atatcgaaga actgctggaa gctggcattg atgttttcac aaccgtcaac gttcagcatc
     5761 tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc
     5821 ccgatccttt tttcgatgcc gccgacgacg tggtgctggt ggacttgccc ccggacgatc
     5881 tgcgccagcg gctgaaagaa ggcaacgtct atattgccgg gcaggcggag cgcgccattg
     5941 aacatttttt ccgcaaaggc aatctgatcg ccctgcgcga actggcactg cgccgtactg
     6001 ccgatcgcgt tgatgagcaa atgcgcgcct ggcgggggca tcctggcgaa gagaaagtct
     6061 ggcacacgcg cgacgcgatc cttttatgca tcggccataa caccggcagc gaaaactggt
     6121 ccgcgcggcg gcgcggctgg cgtcacggct ggggagcgtc tggcacgcgg tgtatgttga
     6181 accccctgcc ctgcatcgct taccggagaa aaaacgtcgg gcaattctca gcgccttacg
     6241 tctggcgcag gagctgggcg cggagacggc aacactttct gatccagcag aagagaaagc
     6301 ggtagtgcgt tatgcccgtg aacataatct cggcaagatt attctcggtc gcccagcctc
     6361 gcgccgctgg tggcgtcggg aaacatttgc tgaccgactg gcgcgcatcg cccccgatct
     6421 cgatcaggtg ctggtcgcgc ttgatgaacc acccgcccgc acgattaaca acgcgccgga
     6481 tagccgctca tttaaagaca agtggcgggt acagattcag ggatgcgtgg ttgccgccgc
     6541 gttatgcgcc gttatcacct taattgccat gcagtggctg atggcgtttg atgccgccaa
     6601 cctggtgatg ctgtatctgc ttggcgtggt ggtggcgcta ttttatggac gctggccttc
     6661 ggtggttgcc accgtcatta atgtggtgag tttcgatctc ttttttatcg ccccacgcgg
     6721 cacgctcgcc gtctctgatg tgcaatatct gctgaccttc gcggtgatgt taaccgtcgg
     6781 gctggtgatc gggaacctta ctgctggcgt gcgttatcag gcgcgggtag cccgttaccg
     6841 cgagcaacgc acacggcact tatatgaaat gtcgaaagct ctggcggtgg gccgcagtcc
     6901 gcaggacatc gccgccacca gcgaacaatt tattgcctct acgtttcatg cccgcagtca
     6961 ggtgttgttg cccgatgaca acggtaaatt gcagccgtta acacatccgc aaggaatgac
     7021 gccatgggac gatgccatcg cgcagtggag ttttgataaa ggcctgcctg cgggcgcggg
     7081 caccgacacg ttacccggtg taccgtacca gattttgccg ctaaaaagcg gcgagaaaac
     7141 ctacggctgg tggtggtgga gccggggaat ctgcgccagt tgatgatccc ggaacagcag
     7201 cgcctgctgg agacttttac gctgttagtc gccaatgccc ttgagcggct gacgctaacc
     7261 gccagcgaag aacaggcgcg gatggcaagc gaacgtgaac agatccgcaa cgccctgctg
     7321 gcagcgcttt cgcatgattt acgcacgccg cttacggtgc tgtttggtca ggcagaaatc
     7381 ttaacgctcg atctggcaag cgaaggatca ccccacgccc gccaggccag cgagattcgt
     7441 cagcatgtgc tgaacactac ccgactggtg aataatctac tggatatggc gcgaattcag
     7501 tccggcggct ttaatttgaa gaaagagtgg ttaacgctgg aagaagtagt cggcagcgcg
     7561 ctgcaaatgc tggaaccggg tttatcgtcg cccatcaatc tttctctgcc agaaccgctg
     7621 accttaatcc acgttgacgg gccactcttt gaacgggtgc tgattaatct gctggagaac
     7681 gcggtgaaat atgcgggtgc gcaggccgaa attggtatcg atgcccacgt tgagggcgaa
     7741 aatctacaac tggatgtctg ggataacggc cccggtcttc cgccaggcca ggagcagacg
     7801 atatttgata agtttgctcg cgggaataaa gagtcggcgg tgccgggtgt cgggcttggg
     7861 ttggcaattt gtcgggcgat tgtggatgta cacggcggca ccattaccgc gttcaaccga
     7921 ccggaaggtg gtgcctgttt tcgtgttaca cttccccagc aaaccgctcc tgaacttgaa
     7981 gactttcatg aggatatgtg acaaacgttc tgattgttga agatgaacag gctattcgta
     8041 gctttctgcg cacggcgctg gagggcgacg ggatgcgcgt ctttgaggcc gaaactctgc
     8101 aacgcggctt gctggaagcg gcaacccgta agccagattt gattattctc gatctcggcc
     8161 tgcccgatgg tgatgggatt gagtttatcc gcgacctgcg ccagtggagc ccggtgccgg
     8221 tgattgtgct ttccgcacgc agcgaagaga gcgacaagat cgccgcgctg gatgccggag
     8281 cggatgatta tctgagtaag ccgtttggca ttggcgaatt gcaggcccgt ctgcgcgtcg
     8341 ctttacgccg ccactccgcc accgccgcgc ccgatccgct ggtgaaattt tccgatgtta
     8401 ccgtcgattt agccgcccgc gtgattcacc ggggtgagga agaggtgcat ctcacaccaa
     8461 ttgagttccg cctgctagcg gtgctgctca acaatgccgg aaaagtactc acccagcgcc
     8521 agctccttaa ccaggtgtgg gggccaaacg cggtcgaaca cagtcactat ttgcgtattt
     8581 atatgggaca tctgcgacaa aaactggaac aggatccggc ccggcctcgc cacttcatta
     8641 ctgaaaccgg tattgggtat cggtttatgc tttgaattgt tattcaataa attcgcttaa
     8701 atagtgatta atttattatt aaacctgcgc cgaatattat tatttggcag gtttaatttc
     8761 ttaacgcaaa tataaaacac gaaattacaa catttaagta acaaaacagc atctaatatg
     8821 gcgatattca taattaatga tttattatct ggggtatgat cgttttttgt tgatcttctt
     8881 cacagattat agccatttca tggatagaat aactctacct tcaactgaca cagcaagcgg
     8941 taaaggtaaa tggaaaataa taaccgctta atgcctcata taaggcggac aacccatatc
     9001 atgaagtttg cccatcgtaa tagttttgac tttcatttct ttaatgcccg ctagtcttct
     9061 gactaaaggg caccccaacg tacaggtctc cctgacttta agcattacag gttaatacct
     9121 gtattcctcg gtgctcatat actgctaacc cttttaaact ctaaataatt cgagtcgcag
     9181 cacttgcaac ttgaggtatg acgagtatag ccagttaccg ggctggtctg ggttattgca
     9241 tctgcaaaaa gcaaactact gatttattta tcagcggtgg agctttgctt tttttccggc
     9301 gtgatcgatt tctcctttga gaaattgagg acctgctatt acctgaaata aagagatgaa
     9361 taaaatgtca gaattaaaaa ttgccgttag ccgttcttgc ccggattgtt tttccactca
     9421 tcgtg
//
LOCUS       DAJTXY010000138         9233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTXY010000138 DAJTXY010000000
VERSION     DAJTXY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.138"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(329..673)
                     /locus_tag="OO566_003524"
     CDS             complement(329..673)
                     /locus_tag="OO566_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6606661.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="OO566_003525"
     CDS             805..2715
                     /locus_tag="OO566_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6606662.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQLGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="OO566_003526"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="OO566_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6606663.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="OO566_003527"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="OO566_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6606664.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRRQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="OO566_003528"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="OO566_003528"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6606665.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="OO566_003529"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="OO566_003529"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6606666.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNARQLRTRQLACLQLLAD
                     WRLRKARECDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="OO566_003530"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="OO566_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6606667.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="OO566_003531"
                     /pseudo
     CDS             complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="OO566_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
BASE COUNT         2243 a         2270 c         2465 g         2255 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtagcagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc taggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg gtcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 agacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgatgagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgccaggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagtg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaactgca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 cggtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttg
//
LOCUS       DAJTXY010000139         9230 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTXY010000139 DAJTXY010000000
VERSION     DAJTXY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.139"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO566_003532"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO566_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6606668.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO566_003533"
     CDS             1828..3147
                     /locus_tag="OO566_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6606669.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3433..3474
                     /locus_tag="OO566_003534"
     CDS             3433..3474
                     /locus_tag="OO566_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606670.1"
                     /translation="MVQLVDLARCVSF"
     gene            3512..4657
                     /gene="dosC"
                     /locus_tag="OO566_003535"
     CDS             3512..4657
                     /gene="dosC"
                     /locus_tag="OO566_003535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6606671.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4582..5199
                     /locus_tag="OO566_003536"
                     /pseudo
     CDS             4582..5199
                     /locus_tag="OO566_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5457..5789
                     /gene="ddpX"
                     /locus_tag="OO566_003537"
                     /pseudo
     CDS             5457..5789
                     /gene="ddpX"
                     /locus_tag="OO566_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5803..6699
                     /locus_tag="OO566_003538"
     CDS             5803..6699
                     /locus_tag="OO566_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6606672.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6696..7682
                     /locus_tag="OO566_003539"
     CDS             6696..7682
                     /locus_tag="OO566_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6606673.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO566_003540"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO566_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO566_003541"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO566_003541"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6606674.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2251 a         2254 c         2321 g         2404 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acacttagtt aaacagctat aaatccctaa taaaaatgta tgaatatgaa
     3301 aaaccatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     3361 aacagtagcg tagattgaaa ctgtcttaag tcgcttattt cttttcagta tatcttcata
     3421 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gcgtttcgtt ttgattccgt
     3481 ctgatttccc agccttataa gggtgtgaat tatggagatg tattttaaaa gaatgaaaga
     3541 tgagtggacc ggacttgtcg aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat
     3601 tgcccttgcg catgctcatt atctgagtat cgagttttat cgaattgtcc gcatcgaccc
     3661 gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga
     3721 acgctggatt attaacgtgc tttctgccca ggttgacgat gtcgaaaggc taatacaaat
     3781 ccagcatacc gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat
     3841 ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg attattccgc
     3901 cgcagaaaaa cttcaggtct accatttctc gattaacagt attgatatcg cgatggaagt
     3961 gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg
     4021 tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact
     4081 ttcatgggaa atagatatta tctataaagt cctgctggat tctgatttag gcagcagttt
     4141 gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag
     4201 cggcattgcc gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa
     4261 tcaaaccatg cgtaacacca ggattttgaa taacagaagt ttgcgggtga aatttctatt
     4321 acagataaga aataccgtat cgcagattat taccttgctg cgtgaattgt ttgaagaagt
     4381 atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc gtttcctgcc
     4441 gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct
     4501 gattattgac gttgataaat tcaaagagat caacgatacg tggggccata acactggcga
     4561 tgaaattctg cgtaaagtct cattcctctc gcagaagaga ttggtgaaat cgaaaatatt
     4621 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
     4681 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
     4741 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtggaa
     4801 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
     4861 cgtgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc cggattatcc
     4921 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagctttgt cgatcgttgt
     4981 ctgacggaaa aacgcatcct ggccttactt gaagccatta ccagcattgg gcaaagcctc
     5041 aatttaaccg tcgtggcgga aggggtcgaa accaaagagc aatttgagat gctacgtaag
     5101 atccactgtc gcgttattca gggatatttc ttttcccgcc cccttcccgc cgaagaaatt
     5161 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact
     5221 cgcgggtttt cctttaaggt gacactgtca cttaaatact gtgatttcag ctacgattcc
     5281 gggagattcc ttccttaact ttccctcttg caccaaaact gcactacgct gcacaaatgt
     5341 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
     5401 atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa ccgtttatgt
     5461 cggataccac cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga
     5521 aatatgcctg cgttgataac atcacaggta aagctattta tcagcaagcg cgttgtctgc
     5581 tacataaaga tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctgtcaggct
     5641 tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag
     5701 cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca
     5761 cggcgctcga cctgacgctt cgtgatgacg tggaggtggt gaatgatgct aagcgaggaa
     5821 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
     5881 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gcgactgatg
     5941 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
     6001 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttt
     6061 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtggt ggtcgccatt
     6121 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
     6181 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
     6241 gcactggctg ccgctctcgg gccgagttta tttaacgcca tgctggcgat tgctattgtg
     6301 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
     6361 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
     6421 ttacgtaact ccctaccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt
     6481 ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg
     6541 ggcgcgatgg tagcgaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt
     6601 ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc
     6661 gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc
     6721 aacagctgca tttgagtttc cccggtttta acggtgatgt tcacgcgctc aacaatgtgt
     6781 ccttgcagat taaccgcggc gaaattgtcg gtctggtggg agaatccggc tcaggtaaat
     6841 cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg
     6901 ggcagatttc actgctggga gaagatgttc ttaacgctcg ggaaaaacag cttcgtcagt
     6961 ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctt aatccgacac
     7021 gtcgaatagg tcttcagatg atggacgtaa tccgccatca tcaaccaata agtcgtcggg
     7081 aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat gccgtggaag
     7141 ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc
     7201 tggcattctc ctgcgagccg caattgatta ttgccgacga accgacgacg gcgctggacg
     7261 tcacagtaca gttgcaggta ctgcgtttgc ttaaacataa agcacgcgcc agtggaactg
     7321 cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg
     7381 tgatgtatgc cggaagcgta attgaaagcg gcgggacggc agacgttatc catcatcccc
     7441 ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc
     7501 cattacccgc cattccaggg acggtaccaa acctcaccca tttgcctgac ggctgcgctt
     7561 ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggtaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttactgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gagctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgagctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat
//
LOCUS       DAJTXY010000140         9040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTXY010000140 DAJTXY010000000
VERSION     DAJTXY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.140"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO566_003542"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="OO566_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6606675.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="OO566_003543"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="OO566_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO566_003544"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO566_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6606676.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="OO566_003545"
     CDS             complement(2403..2606)
                     /locus_tag="OO566_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606677.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="OO566_003546"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="OO566_003546"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6606678.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="OO566_003547"
     CDS             complement(3802..4014)
                     /locus_tag="OO566_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6606679.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="OO566_003548"
     CDS             4274..4582
                     /locus_tag="OO566_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6606680.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO566_003549"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO566_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6606681.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO566_003550"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO566_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6606682.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="OO566_003551"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="OO566_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6606683.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="OO566_003552"
     CDS             complement(8478..8981)
                     /locus_tag="OO566_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6606684.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2240 a         2314 c         2149 g         2337 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcagcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagaacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc agtggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctac tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaaat
//
LOCUS       DAJTXY010000141         9012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTXY010000141 DAJTXY010000000
VERSION     DAJTXY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.141"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO566_003553"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO566_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6606685.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO566_003554"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO566_003554"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6606686.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO566_003555"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO566_003555"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6606687.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO566_003556"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO566_003556"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6606688.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="OO566_003557"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="OO566_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6606689.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /locus_tag="OO566_003558"
     CDS             5545..6018
                     /locus_tag="OO566_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6606690.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="OO566_003559"
     CDS             6047..6589
                     /locus_tag="OO566_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6606691.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="OO566_003560"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="OO566_003560"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6606692.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="OO566_003561"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="OO566_003561"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6606693.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="OO566_003562"
     CDS             7986..8243
                     /locus_tag="OO566_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6606694.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="OO566_003563"
     CDS             complement(8219..8773)
                     /locus_tag="OO566_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6606695.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2255 a         2190 c         2248 g         2319 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaacg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaac gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatgacaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taatatcatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggatccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag ag
//
LOCUS       DAJTXY010000142         8989 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTXY010000142 DAJTXY010000000
VERSION     DAJTXY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.142"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..2886
                     /locus_tag="OO566_003564"
                     /pseudo
     CDS             1..2886
                     /locus_tag="OO566_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2996..3844)
                     /gene="alkA"
                     /locus_tag="OO566_003565"
     CDS             complement(2996..3844)
                     /gene="alkA"
                     /locus_tag="OO566_003565"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6606696.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3978..5330
                     /gene="yegD"
                     /locus_tag="OO566_003566"
     CDS             3978..5330
                     /gene="yegD"
                     /locus_tag="OO566_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6606697.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5342..7282)
                     /gene="yegI"
                     /locus_tag="OO566_003567"
                     /pseudo
     CDS             complement(5342..7282)
                     /gene="yegI"
                     /locus_tag="OO566_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7279..8040)
                     /locus_tag="OO566_003568"
     CDS             complement(7279..8040)
                     /locus_tag="OO566_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR6606698.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8037..8696)
                     /locus_tag="OO566_003569"
     CDS             complement(8037..8696)
                     /locus_tag="OO566_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6606699.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2185 a         2388 c         2346 g         2070 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgtgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtggtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct ctgtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc caattttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggttacgaa
     2101 ccacagcaag ctgccgcaca tagcgaacgg gcggcgatgt cgcttgatga acagtggcgg
     2161 atgattaaag agaatcagtt gatgatgatc gcccacggtg tcgcttcgcc gcggatcccg
     2221 gaagcgcgta atttgtggct gatttcactt aagctctgga gttgcgaagg cgagattatt
     2281 gatgaacaaa aatttcgtcg tagcttcagc gatccggcac ttagccatgc tcttgaccga
     2341 cgggtattcc acgaattttt ccagcaggcc gcaaaagcgg ttgccagtaa aggcataagc
     2401 atcgccctcc ccctttccgt tgccggtttg agtagcgcca cgctggtgaa tgatctgctt
     2461 gagcagctgg aaaatagccc tctaccacca cggttattac atctgattat tccggctgaa
     2521 gcgattttag atcacgcaga aagcgtgcaa aaactgcggc tggcgggatg tcggatagtg
     2581 ctcagccagg tgggccgcga tctgcaaatc ttcaactcgc tgaaagcgaa tatggcagat
     2641 tacctgctac ttgatggtga gttatgcgcc aacgtgcagg gtaatttgat ggatgagatg
     2701 ctgattacga ttattcaggg gcacgctcag cgactcggga tgaaaaccat cgccgggcca
     2761 gtcgttttac ccttagtgat ggatacgctt tctggcatcg gcgtcgatct gatttatggt
     2821 gatgtgattg ccgatgccca accgctggat ttgctggtga atagcagtta tttcgcgatt
     2881 aactgagaga gcgtttgccc attgcctgat gcgatgctaa cgcgtcttat caggcctaca
     2941 aatcgctcat tccgcaggcg ggataaggcg ctcgcaccgc ttccggcgac caacgtcatg
     3001 cttcgtctgg ttgccagcct tccgtatacc agatatgcaa cagcgcataa gaacgccagg
     3061 gcttccagcg ctcggcataa cggcggattt gtgccggtgt catccccaga aatcgctgtt
     3121 taatcagata atcatccggc agaaaaacat ctttcgcctg ccagccgcgc aaggcgaaat
     3181 aattcgccgt ccagcgcccg atacccggaa aagtttgcag cgttttcatc gcctgctcca
     3241 catcgcccgg tattgtcatt ggtaaggtgc cctccagcgc cgcatttgcc agatgaatca
     3301 gcgcctctgc ccgtttcaac ggcataccta acgcttttaa tgcctgcggg tcggctgctg
     3361 ccagccgctg aggcgtgggg aagcagatat actccggaaa atcatccagc cgttcgccat
     3421 aaagctgtgc cactctggcg gtcaattttg ccgccatcgc cacgctcacc agttggccta
     3481 aaatcgcccg cacgccctgc tcaaaagcat caacactgcc gggtaaacgc aatccgggcc
     3541 gcgccgcgcc taacttgccc aacgcaccgt taacaatctg cgggttacat tgcagatcaa
     3601 gcaggcggct cattttcgcc agacactcag cggcaacggg ttctaaacct gcacttaaat
     3661 ttatgtgcag agtatggcgg gctatatccg gaatagcagt caccacgccg cgatattcgc
     3721 ccactgccag actacgggca taataactgt ccgcgaccgt ttccacacca ctcaccgcac
     3781 gggcggcgag aaatcccaac atccacgacc agtcatacgg cggctgccag ttcaggatat
     3841 acatcgcatc tccttattca tccgctttca gcataaacgt tattcagacg ttacgctttg
     3901 ctttcatatt ccggttgtcg cgacggcaac atttcgctaa agtcacgccc cttcttcacc
     3961 ggcatgggga ttatttcgtg tttattggtt ttgattacgg tacagcaaac tgttcagtgg
     4021 cggtcatgcg tgacggtaaa ccgcatttgc taaaaatgga aaacgacagc acgctgctgc
     4081 cttcaatgct ttgcgcgcca acgcgtgaag cggtaagcga atggctgtac cgccatcatg
     4141 acgttccggc agacgacgat gaaacgcagg cgttgttacg acgggcgatt cgttataacc
     4201 gcgaagaaga tatcgatgtt acagcgaaaa gcgtgcagtt cggtctttcc tcactggcac
     4261 agtacattga tgatccagaa gaagtgtggt ttgtgaaatc accaaaatcg ttcctcggtg
     4321 ccagcggctt aaaaccgcag caggtagcgc tgtttgagga tctggtctgc gcaatgatgt
     4381 tgcacattcg ccagcaggcg caggcgcagc tgccagaagc aattactcag gcggtgattg
     4441 gtcgcccgat caacttccag gggctgggcg gcgatgaagc aaacgcccag gcgcagggga
     4501 ttctggaacg cgcggcgaag cgtgccgggt ttaaggatgt ggtattccag tacgagccgg
     4561 tagcggctgg gctggattac gaagccacct tgcaggaaga aaaacgggtg ctggtggtgg
     4621 atatcggcgg tggtacgact gactgttcat tgctgctgat ggggccgcag tggcgttcgc
     4681 gtctcgatcg tgaagccagt ctgctgggtc acagtggttg tcgtattggc ggtaacgatc
     4741 tggatatcgc cctagcgttt aaaaacctga tgccactgct gggtatgggt ggcgaaaccg
     4801 aaaaaggcat cgccctgccg atcctgccgt ggtggaatgc ggttgccatc aacgacgttc
     4861 ctgcacagag tgatttctac agtagtgcca acggtcgtct gcttaacgat ctggtacgcg
     4921 atgcccgcga accggaaaaa gtggccctgt tacagaaagt ctggcgtcag cgtttaagct
     4981 atcgcctggt gcgtagcgca gaagagtgca aaattgctct ttcaagcgta gcggaaaccc
     5041 gcgcctcact gccgtttatc agcaatgaac tggctacgct gattagccag cgagggctgg
     5101 aaagcgccct cagccagcca ctggcgcgga ttctggaaca ggtgcaactg gccctggata
     5161 acgcccagga aaaaccggac gttatctacc tgaccggcgg tagcgcccgt tctccgctga
     5221 ttaaaaaagc gctggcagaa cagttgccgg gcattccgat tgcaggcggc gatgactttg
     5281 gctccgtcac cgccgggctg gcacgctggg cggaagtggt atttcgttaa aagaaaatgg
     5341 ctcagcagcg gcttaaatca gcctgcgcct gagccagttt ttctgccgcc tgacgtaacg
     5401 gttccatcaa cggcatggtc cgtgctggag catgaaggcg gaatcgctgc aactctgtcg
     5461 cgccgacagt caacgtcgat tccagccgat gtcgtttggc ggccattttc gccattaccg
     5521 cctgtctgtc tgctggcgtt accgcttcgt tcggcctgaa cacaaaacgg cgttcacagc
     5581 tcgctttcca gtcgatgacc gcctgggtca gatgatcacc aaacccttta acttgcttaa
     5641 cgccacgacg ggtaacacct gctgctgttt caataccaaa agaccataac gccgctttac
     5701 gcgcagggcc aacgccggga atagaggcaa catcaataaa aaatccctcc agaaacttct
     5761 acttctgccg ttcccttgca gtgtcatgaa gtgctgccag agcgagtttc tcttcttccg
     5821 gtaacccgag aatttcgtcc ttcatttttt cgagcatcgt ccgtttggcg ataaaacctt
     5881 ccaggccgcc tacctgctgg atctggcgga ctaaatggtc atagtccatt ttcgcacgat
     5941 taaatgcctc acggcgttgc tgaacttctg ctttgtacgc tttgcttgtc agactgccaa
     6001 taatccagat agccgccagc acaggaacca aaataatata acgcggttct gcctgaaggc
     6061 cgcaaagcag cagcgataat gctgacagtg cgatctcaag cagaatgatg tattcgcgcc
     6121 gtaacaggcc taaaggaagc ggcctgccag tcggttggaa atgatcgggt aatggcagtt
     6181 gcaatgctgg cggtgctact gacgccatca ccatcgccca gacttttgcc agcacaaaat
     6241 ccccgccggt ggtaatgacc tcttcgccga gatcaataaa atagataacg ccttgattat
     6301 ccagcgtaca ccacaggcag tcggtcaaat gagcgggata aacatgcatt gccgaaacgg
     6361 gacatttctt taactgttgg cgcagagaat caagcgccgc tacccacgct ttagccgtcg
     6421 gacgcccggt tgccacgcca ctttccgtga atgcctgctg aaacatggct tcaacatcac
     6481 tcggtaacat cgatagcgga atcgatcgtg gcggcggttt taaaccacgt cgctgattat
     6541 ctgacgcgta ggcataacgg aaatgggtaa tatccgtctc cagcgcatta cccgcatcag
     6601 agataagcgg cacaccagaa taaggatgcc ggccaccaaa caagacgtga aaaatcagca
     6661 acgcaaggcc aaaattaccg tgattttctg tgcgttcaaa gccgacaaat gacgacaagg
     6721 tttgtagctc tggcggcgta aaatgcgaca cgccgacttc gcataaatgc agtgtgccat
     6781 tggcgttaat ctgaaaggag tcgctatcaa tcaacaccac tttgctgtcg cgacctacca
     6841 taaaactgtt ctggtttacg tcacccacga catgcccgtg ctcgtgaacc gtagcaaaag
     6901 atgaagcaat attgcgcgca acatagagta gaaaatccca cgcacaatga gggtaacgtt
     6961 gacgacgatg tgccgggcta tagatcatat gaatcggttc tttaccagaa acttttggca
     7021 tcataaaacc gataactttt ccgcctcgtc caccgtgaag cgttgcctgc ggccaggcga
     7081 cataattcaa caattgcgcg tcagctgtcg cagccataaa ggcaagtttg tcctgtttta
     7141 aggcgggtgg cggcgtgtga taaatcttgg cgacgctatc gacaaactca ttgatatcat
     7201 aaaccgcgcc ttcaccgcct ttgcccagtt cacggcctgg cgtaacgcac tcgccagtag
     7261 cagtatataa agtgggtttc atggtaacca cagcgccagt gccaacgttt tatcatcatc
     7321 cgtacgctcg tttaccgccg ggctggacaa aaactgcttt aacaattcag gtaataaatc
     7381 gagttgttcc tgcgttgctg acgccagtcc attgaaaaac ggggtaaaga aaggcacatg
     7441 cggagaatta tccaacatat ttaacgccag tcgctggata ccatccgtaa atgctgcaac
     7501 tttatgcaca cgctcagtgc tggtgaaagt ttccagtctg gaaacggcat cttcatcggt
     7561 aatgaagtgc gtcatattgg catattcacc caccatcggc gtaagcggca attgcagacc
     7621 gtgaccaaaa tccaccacca cgccgccatc gccaatctgc ataatcaagg tgccattagc
     7681 cgatgaaatc aatcccagaa acgtgcaggc aaaatcgcga actgctaatt ctttggcttc
     7741 cgcctcggca aaaagtcgct gacgaacggt cagcacaata tccgtggcga ggatatcatt
     7801 aagccccaat tccccaccct gcactttttg cgacatataa gccatcgctt cattgacggc
     7861 gagcatcgcg ccttcgcccc cctgcgaaac gctacctgcg ccatcagcaa ggaacatcac
     7921 cagtaatggc tgctggtcat ttagccaggc aacctgcatc tggcaggcat cctggcaagg
     7981 caagtccgcg ctgatgtgcg acgttccaac agcggatgca taaaccagac gccagctcac
     8041 actgacgtcc agccttttgg cgcttccagc acaacttccg ttcccggcgt ggagcgggaa
     8101 accgaacgca gcgaactgga taaccagcta aacagttcac ggaattgtaa tccttgcaga
     8161 ggcaaaggct gacgaacgct gatttgtgcc agagtcttca tatccgcccc ctgaacaccg
     8221 atggaaaaga aagcaaacct tttatcttct tccccccgaa acactttgtt ggctgcagcc
     8281 tgccactcat cggttggtgc accatcggta atcaggaaaa tccacgggcg ataatacgaa
     8341 ataccatttg cacgatactc acgtttccgc tcctctacca tatccagggc tttggtaatg
     8401 gccgcaccca ttggtgtatc gccctgggca aacaggatgg gcggaaaaaa attagcggcg
     8461 ctggtaaagg gttgttccac atgcaccggg ccgaacgtca caatgccgag ttccactctt
     8521 tttaaagcca gcgaatcggc aagcagctca tcgcgaaagg taaccaatcc ggtgttaagt
     8581 tcattaatag gtctgccact catcgagcca gaaacatcca gcagcagaat gcaggggcaa
     8641 cgtggttccg ggttactggc aaaatcgctg gtggcaaacg tgatttgttc gctcataaga
     8701 aatcctttct tagagaatta ttgaggtccg gtatcatacc gactgcaata atgaaataag
     8761 agcaaacgaa gaaactttca gaattagtcc cggcggcgca gaaaattttg ctattcgcct
     8821 cgcaagaacg gagagccgtc gattgacact aagggcggag tgacataatt tcaggagtga
     8881 gggttaggga gaggtttccc ccgttacttt attcgtaccc cttataatgg ggtgttagcc
     8941 agccagaccc ggcatgatta ctgcccccag tcgtccatga tccgggggg
//
LOCUS       DAJTXY010000143         8922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTXY010000143 DAJTXY010000000
VERSION     DAJTXY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.143"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            67..391
                     /locus_tag="OO566_003570"
                     /pseudo
     CDS             67..391
                     /locus_tag="OO566_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(421..3333)
                     /locus_tag="OO566_003571"
     CDS             complement(421..3333)
                     /locus_tag="OO566_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606700.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3336..3452
                     /locus_tag="OO566_003572"
     CDS             3336..3452
                     /locus_tag="OO566_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6606701.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3816..4658
                     /locus_tag="OO566_003573"
     CDS             3816..4658
                     /locus_tag="OO566_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6606702.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4661..5749
                     /gene="capU"
                     /locus_tag="OO566_003574"
     CDS             4661..5749
                     /gene="capU"
                     /locus_tag="OO566_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6606703.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNEPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5754..6704
                     /gene="virK"
                     /locus_tag="OO566_003575"
     CDS             5754..6704
                     /gene="virK"
                     /locus_tag="OO566_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6606704.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6769..7713
                     /gene="lpxM"
                     /locus_tag="OO566_003576"
                     /gene_synonym="msbB"
     CDS             6769..7713
                     /gene="lpxM"
                     /locus_tag="OO566_003576"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6606705.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(7867..8899)
                     /locus_tag="OO566_003577"
                     /pseudo
     CDS             complement(7867..8899)
                     /locus_tag="OO566_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2332 a         2048 c         2136 g         2406 t
ORIGIN      
        1 tgcaggcaaa ggccatgaaa gccctgattt ccggaagcga gcgaacagtc cacaaactgt
       61 tgaacgtggt gtcagcggcg gacagggata agccctcgtc agcagttccg tcagcactgc
      121 gacagcgttc tttacccggt caaaacagcg ttgcggtgca ccacgcctga cggatgaact
      181 gcgtgctcag ggttaccact ttaacgtaaa aaccgtggcg gcaagcctgc gccgtcaggg
      241 gctgagggca aaggcctccc ggaagttcag cccggtcagt taccgcgcac atggcctgcc
      301 cgtgtcagaa aatctgctgg agcaggattt ttacgccagt ggtccgaacc agaagtgggc
      361 gggagacatc acgtacttac gtacagatga agttcggtta catcctgtca gcacggaacc
      421 tcacgctttt tgagcggcac gacggatccc ccccacccag tatccgacag acttttcact
      481 gcgttcgtaa aaacccttct tccctcccag catatccact aattccggtg acatatcctg
      541 cagataagtc tgcaaatcat gccccagatc ttccggtgac tgcaactcat aaaccgcgcg
      601 ccacacatca ccctgcctta ttttaagccc cgggttaaga atatactgtc cgtggttcag
      661 cctcagaaaa agcttttttc cataaggatt tttgctgtta acttcatgtt gagagagctg
      721 gctgctgata taactccttc gtttgcggcg ttccggcagc aatgcttcag gaagatgaac
      781 cacacactct gccagtcgtg ccgcactgaa ggcttccgaa aaaaacagag cacttcccag
      841 atgcgtgtgg aacagcgcct gcatcaggat caccagaaac attgccatct gccggttgtc
      901 cagtttgatg aggcgtcctt ccacctgcag tgacagactt tcaggtttca gcagggtata
      961 caactgcgca cttttctgtt gcgcataacg gggcgtactg accgccgctg acagaacttg
     1021 atgataagcc gccagcccgt cattaccggt cagcaaggga tctgcaccac gctccagcag
     1081 taactgaacc agtgcaatat tacctgcccg ggctgctgac atcagcggag taagattaaa
     1141 cggcgaacgg tgatcaacac cataacgttc aatatcccgc aacacggcaa ccgggttatt
     1201 cattgagtat ggccggaaat acttcagctc cagtaactgc agagtcttcg ttctgggctg
     1261 ccttgtgggc ttaaaatctt cccggtacag tgcattatac agcgggaaca gggaataaat
     1321 cagactgtac tccagcgcct gcagttgtat ggttttatcg gctgtacctg cgaggatatg
     1381 ctgtttccac tgtcgtgcat cctctgcggt gataatctgc catggcatct cagatgtctg
     1441 caggatccgg ctacggatgg tgtccgcctg ctcctgcttc ccctgttttt caagcagatg
     1501 tgcggttttc tgccactcat cacggctgga aatctcttcc tgcagattca gaggctcttc
     1561 ctgatgcgtg agcgcaagca gactccacag aggatgctcc acctgctgtt caacaagata
     1621 cacattgtgt gtagcacggg tgagcgcaac atacagcgcg ttggtaaaaa atttatagat
     1681 ttccgctgaa cgatcctgcc tgtcccgcgg acgggaataa cgggcctcgc cctcaagatc
     1741 agccggtgta agaccctcgc aaatatcatc aaatgcctgc cgggcagcgc ttactatatt
     1801 gtaaaggatc accgtttcgt attccagccc cttaacctcc tgaaccgaaa agaccagcgg
     1861 cgtactgaac cagcatcgcg cctgtgcttt ctgttccgga tgcatcacaa tgaccgctac
     1921 acgattcgaa agtgaggttt tggcattcag ttcctgcttt gtctcttccc tgtcgtccag
     1981 caaccggatg ctcccttctg cctgaccgca gcttctgaca aaatggtgac tttcccgatc
     2041 aatagcacta aagcggacct gtttgagtct cagcaaccgg ttagccagtg cagtgacgtg
     2101 gtgcccgtta cggtaattcg cctgcaggat attaacggtt gtgtcgtttc cctgttgctg
     2161 ccggaaaaag agacttttga gcgaactcca tgagaaaaag ctcggatgaa ctatctgatt
     2221 ggcatcacca cagagtaaaa actgaccggg atgacgtagc gttttcagta ccagttgcag
     2281 ttgaacaggc gtcatatcct gaacttcatc aacaaaaatg acatcataac ggggagttgc
     2341 caacgaaaga tactgatgac tgagcagatt actgtcatac tgatgcgact gtttcagcca
     2401 tgcgatataa cgttcaaaca ggacatagac cgtcgggcgg tcctccatgc catagagcga
     2461 ctgccgtatc cccagtgaca gatacgcctc ccgggacagc ggcccattcc cggaggcaac
     2521 cgcaccgatc acgcccctga actcctcata cagggtatgg gcagccccca gtgcggcgat
     2581 agccctgttg cggggcagcc agtcgctgaa cgcactgaaa gtcacctccc tgccttccgg
     2641 aatgcgcaga gtttccagaa attccgtcag cgaaagaaag tcaatattct gtacctcact
     2701 cccttcccct gaagcgtcat agagagtacg cgctttttcc accagaaatg aggaaaggct
     2761 gagatacagt atatcaccag cagcctgctt cattttttcc agtaccagcg ccgtcttacc
     2821 acttcccgcc gagccaacaa tcaccagcgg tagcgggtgt tcgtacagtg cctgctgatc
     2881 atcatcaaag gacagcattt tatccaggcg gtggaaacgc ccatgagacg gattgatata
     2941 cgtcagcgct tcagtcgcga tatcaacagg atccggcact ggttgttgtt gtaagcgccc
     3001 ttcatcaatc accacgtttc tgcgaagaaa acgtgaagtg ttataggcat gattgcgaat
     3061 atattccaga acaaggcaga tagtttcacc gtggtagcga tacagcgaaa acagtaatcg
     3121 gtcccggata ctgagccggg cgcggtaaag attggtgtca attttacgaa catcagcctg
     3181 aacaaagtta tcggtggcaa tagcctgtgc aaatttttta taacccggga tcgtgtcagg
     3241 attcagagta tgataaagac agattttcat ggctgccggt ttccgtcgtg caaaaaacat
     3301 gctgatttta cgaaggaatg ccttactgaa caaccttgcc cggaataccc cccgaaatca
     3361 gatggaacaa tcagaaaaat gctctgcagt tatggaataa agaaggtggg gtggtacgcc
     3421 gggagggcga tccgctggaa atggcgcagt agctgcaggc ctgtcatgat gccggaatag
     3481 aagttcgtgt acaggttaat gaaagcgtca ccccgtaaat aaactctgcg ctgaaattcc
     3541 tttttttcat tggagcacct gtgttgttct gaggtgaaca tatcacctct gttcaggtag
     3601 ccaaattcag tgtgccacct caaggttctt agtgagtcgt ttttaaaaga tatgataata
     3661 tatcattgtt tatgaattgt tgatgtttaa tttagtaaat tatatcaaat atgccatggg
     3721 tttgctatgt tattcatcca gtatctggaa ataaaatgca ccagctttat tgggttttcc
     3781 ttttgtctct gcttactagg tgccagaaac tttaaatgtt aaacgaaggg ggcattttgt
     3841 tcaaagccaa tcatgttccg gtattaatgt atcatcatgt cagtcattgc cccggactgg
     3901 ttacactttc tcccgtgaca ttccggaagc aaataaaatg gttggctgaa aacaactgga
     3961 aaacactgtc atcagatgaa ctggagtttt tttatcgggg cggaaaactc ccccggaaaa
     4021 gcgttatgtt gacatttgat gatggatacc ttgacaactg gtttcaggta tatcctttat
     4081 taaaagagtt taatctcaaa gcacatattt ttttaattac cggttttatt gggaatggtc
     4141 cggttcgtca ctctcctgga aaggaatatt ctcatcgcga ttgcgaacat caaatagcga
     4201 cagggaatgc tgataatgtt atgcttcgct ggtctgaagt aaacgaaatg ttgcaatctg
     4261 gtctggtcga atttcatgtt catacgcata ctcatacacg ctgggataaa aagttcagtt
     4321 cacgagaaga acagtgtaag catcttcggc aggacttgtt gagcgggaga gaatatttga
     4381 aagaaatgac aggaaaatgc agtaaacatt tgtgttggcc ggaaggttat tataacaagg
     4441 attatattca ggtcgctgaa gaacttggat tctattattt atatacaaca gaaagaagaa
     4501 tgaatgctcc tgctaaagga actacacgga taggaagaat aagcaccaaa gaacgggaaa
     4561 gttgcgcatg gctgaaaaga cgactttttt attatacaac accgtttttt tcatcgttgc
     4621 tggcctttca taaggggcca agattaccgg atgattaata atgaatatac tatttacgga
     4681 atcatcaccg aatataggtg gtcaggaatt acaggctgtt gctcaaatga aggccctgaa
     4741 gaaaatgggg cattcagttc tgcttgtctg cagggaaaac agcaaaattg cttttgaagc
     4801 cagtaaattg ggaattgata tcacattcgc gttatttcga aacagtcttc acatccctac
     4861 tgcatggaga ttactcggaa tagttcatgg ttttcagccc aatgcaatcg tttgtcacag
     4921 tgggcatgat agcaatattg ttggtttagt acggttattc actaggaaac atccattcag
     4981 aattatcagg caaaagacat atttgacacg aaaaacaaaa gttttttcaa taaatcattt
     5041 ttgcgatgag gtgattgttc ccggaacaag tatgaagaca catctggagc aggaaggatg
     5101 tcgaacccgg gttactgttg tgcctccagg ctttgatttc cagaaattat acgttgattc
     5161 ccgaaacagt ttgcctccaa atgttctttc ttggctggcg tcccgaaggg gatgccccgt
     5221 tattgctcag gtaggaatgt tgcgcccgga aaaagggcac gaatttatgt tgaatttact
     5281 gttccattta aaaatgaatg gacgacagtt ctgttggttg attgtggggt ctggttcgcc
     5341 tgaactgcgg gagcatttac agtatcagat tgacagtatg ggcatgcatg atgatgtttt
     5401 tattgctgac aatgtttttc ctgccgcccc cgtatatcgg gttgccagtc tggtggttct
     5461 gccttcagaa aacgaatcgt ttggtatggt actggcagaa gcatcggcat tttctgtgcc
     5521 tgtactggcc agtcagattg gtggaatccc tgatgttatt cagaacaacc agaccgggac
     5581 attgttacca gcaggtaata agcacgcatg gatgtgcgcc ctgaatgatt tttttaatga
     5641 acctgggcgt ttttatcaga tggctcgcca ggcaaaacag gatatagaag agcggtttga
     5701 tattaataaa actgcgttaa aaatactcac attagcgaag cacaagtaac aatatgtttt
     5761 ctgtaagtaa cttatcattt atcggtttcc ttaaaaggat tgttttttcc tcagattcac
     5821 tcccggggaa gtgggaacac agaaaattcc ggttcatgta cattttgcga tgtgctataa
     5881 atccggttgc cagtattcga tattattacg aactgcgttc cttgcagtgc attgaggata
     5941 ttctggcaat acaacccacg ttgccagcaa gaattcatcg accttatttg cataaagggg
     6001 ggcgagcctg gtcccggggg caatatattc ttgagcatta tcgctttgtg caaaatttgc
     6061 cagaaaaata ttctgaattt ttgtttccac aaaaatcggt atcattagtc caattcatcg
     6121 ggaaagatgg agaggatttt gatatccagt gttctcccag tggttttgac cgggaaggag
     6181 agttgatgtt gtcattgttt ttcaatcaaa tagtgattgc ccggttaaca ttttcagtca
     6241 ttctgactca aaatggacac accgctttta ttggcggatt acagggagcc cccaaaaata
     6301 ccggacctga tgttattcgg tgcgcaaccc gggcctgtta tgggcttttc ccaaaacgga
     6361 ttatttttga agcattttgt gcgttgatga aagcttgtaa tgtctctgag tgtctggcag
     6421 tcagtgagca cagtcatgtt ttccggcaat tgagatactg gtatcagaaa cgcaaaacct
     6481 ttgttgctgt ctacagtgat ttctgggagt ctgtagcagg gaaaacctgt ggtgactggt
     6541 acaaattacc aacacaggtg gttcgaaagc cgctaagcaa tatagctagt aaaaaacgct
     6601 ctgagtatcg aaaacgatac gctttgctgg attatatcca tgaaactgct atccgctctt
     6661 tggatgcata tcctgtaaac tcagaacatc aggacttaaa ttaaggtttg atgtattctc
     6721 tgaataaaat attaatgatg attttggtag gggcattcgc actaaataat gaaaaaatac
     6781 aaatccgagt ttattcctga atttaagaaa aattatcttt cccctgttta ctggtttaca
     6841 tggttcgttt tgggaatgat tgcaggtatt tcaatgtttc ccccttcatt cagagatcct
     6901 gtcttggcca aaatagggcg ttgggtgggc agattgagca gaaaagctcg tcgcagggcg
     6961 acgattaatt tatcgctttg tttcccggaa aagagtgata cagaacggga aataattgtc
     7021 gacaatatgt ttgccacagc attgcaatcc atagtgatga tggcagaact ggcaattcgt
     7081 ggtccggaaa agttccagaa acgagtgttc tggaaaggcc tcgaaattct tgaggaaatc
     7141 cggcacaata acagaaatgt gatttttctg gttccccatg gctggagcgt ggatattcct
     7201 gcaatgttgc tggcagctca gggggaaaaa atggccgcca tgtttcatca gcaacgaaat
     7261 ccagtgattg attatgtctg gaattctgta cggcgtaaat tcggggggcg cttacattcc
     7321 cgggaggatg ggataaaacc atttattcag tcagtacgcc agggatactg ggggtattac
     7381 cttccagatc aggatcatgg tcctgaatac agtgaatttg ctgatttttt tgcgacctat
     7441 aaagcgacat taccaattat tggacgtctg atgaacatca gtcaggctat gattataccg
     7501 cttttcccgg tttatgatga aaaaaaacat ttcctgacta ttgaagttcg gccaccaatg
     7561 gatgcatgca ttgccagcgc ggacaataaa atgattgccc gacaaatgaa caaaacagtg
     7621 gaaattttgg tggggtcaca tccggaacag tatatctggg ttttaaaatt gttaaaaacg
     7681 cgcaaatcaa acgaagcgga cccgtaccct tgaaattata aagtacaggt atttccacta
     7741 gttgtttctt acaggttacc aatcgaaaca catccccctt ccgtgtgttg cccgtttggc
     7801 acggaggact gtgtagatgc agttcgttaa atttaattta tgcctaaata gctgcgctta
     7861 ataccgctac acttttgcca gcccatgttt gcctccgggg aatgggctga cggtttccat
     7921 aaaatggcga acttttttca acagttgcca cattgagcgg cactgatgat tacgcgttat
     7981 tgtgtcgtga agtgcctgcc atagccgttc aacatgattc acccatggcg agtaaaccgg
     8041 ctgataaatg cccctgaact tcgggttctc cttcagccag ctctgtgttt cccggctttt
     8101 gtggataatg tagttgtcca cgatcagcgt gatggttttc gcccgacggt atgtcgcttt
     8161 aagccgcttc agcaggctga tgtaacagcg ccgaactttt gctgttgccg cccacatagc
     8221 tgactttacc tgtcccgctg tgcagcgctc cggccagata atatttttca ttctgtcccg
     8281 gcgtgaccac ccgtttttgc tgtccgcgca gttgccagtc cgcaccgatt ttgggatgaa
     8341 gatggatatc cacttcatct tcataaaaga ccggatgctc tgcgctgcat tcgtccagtg
     8401 ctttatggat tgctgccatc ttttcatctt tatgcgggtc acggatacgc agagttggcg
     8461 cagcccttcg ccacacaatc cccgcagacg gcaaccagcg gcgaacggtt ccggcattta
     8521 actggcaacc ggttatctca ttgattttta ttgccagcag ttctgtactc cagcgtgaac
     8581 gctggtagcc aaagtcgccg ggagaatgtt ttaccagctc acgtaacagt gtgcagatat
     8641 gctcaaacgg ccagcgacgg gcacgcccgg caggtaatga tttcagtccc tcaacacccg
     8701 actgcgtgaa ccagttaatc cagcgtccaa cagaggaacg ggcgcagcag agcgttctgg
     8761 caacgtcgct gacacggtcg ccccggtgca gcatcagcat ggcagtcagt ctgcgggcat
     8821 aatttttatc gtgtgtttta tggatggctt tctgcatcag gcgtcgttcg tcacgggaaa
     8881 ttggtgctat gatcggcatt gctcagtccg gttggtgatt tg
//
LOCUS       DAJTXY010000144         8773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTXY010000144 DAJTXY010000000
VERSION     DAJTXY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.144"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(5..2548)
                     /gene="mdoH"
                     /locus_tag="OO566_003578"
     CDS             complement(5..2548)
                     /gene="mdoH"
                     /locus_tag="OO566_003578"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6606706.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2541..4076)
                     /gene="mdoG"
                     /locus_tag="OO566_003579"
     CDS             complement(2541..4076)
                     /gene="mdoG"
                     /locus_tag="OO566_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6606707.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4470..5627
                     /gene="mdoC"
                     /locus_tag="OO566_003580"
     CDS             4470..5627
                     /gene="mdoC"
                     /locus_tag="OO566_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6606708.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5635..7116)
                     /gene="clsC"
                     /locus_tag="OO566_003581"
                     /pseudo
     CDS             complement(5635..7116)
                     /gene="clsC"
                     /locus_tag="OO566_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
     gene            complement(7058..7591)
                     /gene="ymdB"
                     /locus_tag="OO566_003582"
     CDS             complement(7058..7591)
                     /gene="ymdB"
                     /locus_tag="OO566_003582"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6606709.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7686..7997)
                     /locus_tag="OO566_003583"
                     /pseudo
     CDS             complement(7686..7997)
                     /locus_tag="OO566_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8118..8450)
                     /gene="csgC"
                     /locus_tag="OO566_003584"
     CDS             complement(8118..8450)
                     /gene="csgC"
                     /locus_tag="OO566_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6606710.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8509..>8773)
                     /gene="csgA"
                     /locus_tag="OO566_003585"
     CDS             complement(8509..>8773)
                     /gene="csgA"
                     /locus_tag="OO566_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR6606711.1"
                     /translation="NSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNG
                     KNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT         2194 a         2278 c         2079 g         2222 t
ORIGIN      
        1 gtttttattg cgaagccgca tccggtttac gcaatgccag tggattgagc tttatccctt
       61 cgtaataact cacccatgaa gaatatcttt caggggaatt ccagacgcgg aaatgcagac
      121 gggccatcgt caccggatcg cttagcagca ccaggcgacg atcgcgattc agcttctctg
      181 gcgtctcgtt aagcgcctgt tcaacgtggc ggtcacgggc gatttccagc accttactgg
      241 cgcggtgacg cgctgtcgcc attgcggttg ccagagcgtt aaatgacggg ttaaacactg
      301 cgtgcataaa gccatcgtca agggagcgtt ggcgattcat ctcaaggaac cgatcggtat
      361 caaccagcac ctgcggcggt gaatactctt ccgggatcag gaacagtttc cagcgtttgg
      421 tgcgcagacc aacggtggca cggctggaaa tcaccgaaac aaacggtgac aggatcaacg
      481 agaagacaat cggtgccagc cagaacagga aacgcagatc cagccacgcc atcccaacag
      541 cccacactaa ccccaacagc agctgtgagc cgtggcgttt gaacgcttcg ccccatgaag
      601 tggagtcatc atcacgctgc ggtgaattcc acaccacttc ccagccaagg aacgcgctga
      661 caacgaagac cgtatggaac agcatgcgta ccggagccag cagcacggaa aacagcactt
      721 ccagcagcaa cgataatgta acgcgccaga agccgccgta ttctttcgtt cctttgcacc
      781 agataagcaa aatgctcaat aacttcggca ggaacaacag caccatggtc gaagcaaaaa
      841 gtgcaatcgc cagctcagga cgccactgcg gccacaccgg gaacaactgc cgtggttgca
      901 ggaagtattg cggttcggtc aacgcatgca ctacctgcaa tgcagtagag agcgcgagga
      961 acataaacca cagcggagcg gagagataag acatcacgcc cgtcaggaac accgcacggt
     1021 gaaccgggtg cataccctta accaggaaca gacggaagtt catcaggtta ccgtggcacc
     1081 agcggcggtc acgttttagc tcatcaagca agttaggcgg caattcttca taagaacccg
     1141 ggagatcgta agcaatccag accccccaac ctgcacggcg catcaacgcc gcttctacga
     1201 agtcatgtga caggattgaa ccggcaaagg aaccttcgcc cggcagcgga gccagtgcgc
     1261 agtgctcgat aaatggtttc acgcggataa tcgcgttatg tccccagtag tgcgactcgc
     1321 caagttgcca gaagtgcaaa ccggctgtaa acagtggccc atacacgcgg gtcgcgaact
     1381 gctgacagcg cgcatacagc gtatccatac cggacgcttt cggcgacgac tgaatgatcc
     1441 cggcgttcgg gttggcttcc atcaagcgca ccaggccgca taaacaatca ccggtcatta
     1501 ccgagtcagc atccagcacc accatgtagc tgtactggct gccccagcga cggcagaagt
     1561 catcgatatt accgctttta cgcttcacgc ggcgacggcg gcggcgatag aaaatctgac
     1621 cttcgccacc gacttcagcg ataagctcca tccaggcttt ttgctctgcg acgcagatat
     1681 ccgggttata actgtcactc agaatgtaga catcaaagtg tttggcattc ccggtggctt
     1741 ttactgattc ccacgttgca cgcaagccag caaaaacacg gttcacgtct tcgttacaga
     1801 taggcatgat caacgccgtg cgatgctccg ggtttaatgg ttcatcgcca actgttgacg
     1861 cagatatact gtatttatcg cgaccaataa gcagttgcag gaagcccatc aacgccgtcc
     1921 agaatccggc ggacacccaa cagaacagta ccgcaaaaag gatcaggata ccggtttgca
     1981 gcatataagg cagaagctgc ataaaggaaa cccacaaatc ctgaccaacc atatccatag
     2041 gattaatcag cgcccaaccc tgataaggaa gaatggtctt catataccag gtcgcgacaa
     2101 cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg gatggtacca acggtacgcc
     2161 acttttgctc gctctcctgc tcttctttgg tcaaacgagc cagatagcgc ggcgtgacat
     2221 cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt ggtacgccac gggtcgggaa
     2281 acatcgagga gcgttttgct tctggcatcg ccttaagctg atcgcgccct tcgtcgtctt
     2341 taattaactg tccatcagca agtgaatctg gccaggcttg ttccagacgc gcctttaccg
     2401 agccttgcgg ggaatcatcc tcccgcgccc aggtgcggtg ttcggcatcc agcgcctgat
     2461 gaacggcgcg gatatcagtc ttcggcaatg ctgctttctc gcttgcggcg atgggcattg
     2521 cgtcaatgta ctcagttgtc ttattcattg gcaggtaact ggtagctcca ggtttcactc
     2581 aacgtctgat cggcattcac cagcgcagca cgcatttcag tggttttctt ggcatctttc
     2641 actttcacac gcatcaccag acgccagcct ttggtaaccg ggttatagcg caccgtgctt
     2701 tcaactatct caccattatc accaatgctg gtttgcgctg tgaccggggt atcctctggc
     2761 agttttttca tctctgcgcc ggtaaaatcg accacaaagg cgatagtacc gtcaggctgg
     2821 cgaatcaggt tcgactgctt cacatccccc gttgaacgac gcgtttgttg cacccatgcg
     2881 ttatctggcg catgcagttt gtcttcatca cggctgaagg tgatggtgta tttaaagttc
     2941 atctctttac ccggctccgg cagctgatcc ggcgtccagt aagcgacgat gttatcgttg
     3001 gtttcatcgt tggttggaat ttccaccagc tcaacgctgc ctttgcccca ctcccctttc
     3061 ggagtcaccc atgcgcttgg acggagatcg taacgatcat cgagatcttc aaagcgggag
     3121 aaatcacgac ctcgctgcaa cagaccaaag cctcgcgggt tttccatgga gaagctgctg
     3181 accgctaaat gtttcgggtt attcaacgga cgccagatcc attcgccatt accagcatgg
     3241 atagacagac cgttagagtc gtgcaactcc ggacgatagt tatttgcagg cgacggttgg
     3301 ttcggcccaa acaggaacat actggttaac ggtgcaaccc ccagtttgcc gactttatcg
     3361 cgcagataga ttttcgactg cacatccaca accgtgtcac gccctggcat aaccacgaat
     3421 ttgtaagcac ctgtcgcacg cggcgagtca agcaatgcat aaatggttaa acgtttatca
     3481 gtcggttttg gacgctcgat ccagaactct ttgaagcgtg gaaattcttc acccgatggc
     3541 aaagcggtat caattgctag gccgcgggca gaaaggccat aaacctgacc tgcaccaatc
     3601 acgcggaaat agctggcccc gagcatgctg acgatttcat cgtttttatc tttgctgttg
     3661 atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt ttaccgtgtc tttgtcatgc
     3721 tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga ttcgtttgac tgcggtggca
     3781 gtcacttcat ttattttgac cggggtatcg aagtacatac cctgatggta gaactcgagt
     3841 ttgaatgggg tcttcagatt gttccagtac gctttgtcat gattaaactg gatctgctga
     3901 tagtccgcgt atttcatgtc gcggaaaacg gagggcaagt tgcttttagg cgcctcatag
     3961 cctttcccgg ctaaggattg agcttgcttt gcgacatcat caatactgaa agcccagctt
     4021 gaagatgtat acagggttaa cattactgca gcactcaacc aacgcatttt catcatttgt
     4081 agtttatgtt tcataattag taagcacttc cccctttgtg tgcttatatc gatccgatct
     4141 attttaatgg ataaccagcc attccgacaa ctgaatcccg gtatcgttca gcacgctggc
     4201 tcatggatta agcacctgat tgcaaccgtt ttcactttcc gtgcttctcc taaagacaga
     4261 agcatcgact tcgtgtaggg tttgccagca aatttattta tcaaccgtct tacagacgct
     4321 tccattctgg ttagtggtag cgatgatgct atgcataaaa accttgaagc ctgcgcctgg
     4381 cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg cataacccga cagggataag
     4441 acgaaaaatc cgagattagt taaccatata tgaatccagt acccgcgcaa cgtgaatatt
     4501 tcctcgactc catccgcgcc tggctgatgt tgttagggat cccttttcat atttctttaa
     4561 tctattcgag ccatacatgg catgtgaata gcgccgaacc gtcattgtgg ctgacccttt
     4621 ttaatgactt catccactcg ttccgcatgc aggtattttt cgttatatcc ggatactttt
     4681 cctacatgct ttttttacgc tatcccttga aaaaatggtg gaaagtacgt gtcgaacgtg
     4741 taggtatccc gatgttaaca gccatccccc tactgacatt accgcaattt attatgctgc
     4801 aatatgtcaa agggaaagcg gaaagttggc ctgggctatc attgtatgac aaatataata
     4861 cgttggcctg ggaattaata tcacacctgt ggtttttact ggtgttagta gtcatgacga
     4921 cgctgtgtgt atggatattt aagcgcatca gaaataattt agaaaattct gataaaacga
     4981 ataaaaaatt ctcgatggta aaactatcgg tgattttttt atgcctcggc atcggctatg
     5041 cggtaataag aagaacgatt tttattgtgt atccacccat tctgagtaat ggcatgttca
     5101 attttattgt catgcaaacg ctattttatt taccgttctt tatcctcggc gcactggctt
     5161 tcattttccc tcatcttaaa gccttgttta ccacgccgtc tcgtggctgt accctcgctg
     5221 cagcattggc gtttgtcgct tatttactca accagcgcta tggcagtggc gatgcctgga
     5281 tgtacgaaac cgagtcggtg atcaccatgg tcctcggcct gtggatggtg aatgtggtct
     5341 tctcctttgg ccaccgttta cttaacttcc agtcagcgcg ggtgacttat tttgttaacg
     5401 catcgctgtt tatctatctg gttcaccacc cgttaacgct gtttttcggc gcatacatta
     5461 caccgcacat cacctccaac tggcttggtt ttctctgtgg cctgatattc gtagtaggga
     5521 ttgcgataat tctgtatgaa attcatttac gcatcccgtt actgaagttt ttgttctctg
     5581 gtaaaccggt tgttaagcgt gagaacgata aagcaccagc ccgttaagcc acatttacaa
     5641 taaccattcc acgggcaata tcgacgccag tctgaccata acccgcttcc agaaactggt
     5701 ggcgggttct tttttgagaa taacctcttt ctttgcatga cgatcaaccc agttgatccg
     5761 tccccacctg tccagacgga gctgccaggc cgcatcgtac tggctctgaa taaagcgttt
     5821 atcaattaac tgtgccagcg tttcgctctc tatcacgaag cccatttcag tattgagcaa
     5881 tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc gttttaccat cgatgctaaa
     5941 agttttagca tgcaggctgg caccggaatt accggttatg ccgcgatcgt gtaacgtact
     6001 gctttgttca cgcgtcggct tgagttcata taattccacg ccatagcgga gcaatttttt
     6061 gcgccagcgc gcgtatccgg catggacgac agcaacatcg ttagcggcaa gagaattggt
     6121 taggatcgca atctttaccc cttttctcac catccgtaag agttgcgcca cacctgcgcg
     6181 tgtcggtaca aaataggaag agataatatc gatgcgttca ctgggtgagc ccatgatatc
     6241 gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg ccctcccctt tcgccggatc
     6301 atcacttaat aaacgtgtct tcgcccagat aagcggcaac gttccatcaa ccagatgatt
     6361 tataaatgga ctggattcca ttttgcgtaa ataacgatgc gtcatggcat cgttatgcca
     6421 ggaggcgggt aactcgatgc gatccgccat ttcaccttcc gggacatcca gcacctgctg
     6481 taagggtgaa accgatttgc aataccagta gcgggcgaaa tcatcggcaa cgtcctctac
     6541 cacggggcct attgccatga catctaaatc cgaaaaaagt cgctcttccc ctgcactaaa
     6601 ataggcatca ccaatattta gtcctcccac tagggtaacc acgccatcga cagtgaaact
     6661 tttattgtgc atccggcgat tgagacggga aaagtcggtg atatatccaa gcggacgtag
     6721 caagcgaaac gagaaaggat taaaaagccg gacttcaatg cgtggatgac tgtcaagcaa
     6781 gcgtaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc agcaacaaac ggacgcgaac
     6841 gccacgcttt gctgcggcta acagggcgga aaacagtaac cgccccgaca tatcgtccta
     6901 ccagatgtaa tactgaacat ccagcgtatg ctctgccatt tcggccagac gataccgggc
     6961 ggcaaacgca tccagacttt tctccagagg gaaaagacca cactgaccgg gatgttgcga
     7021 acacagtggc agcaccgcgc tcgccagccg gggcaaatca ttcatctcct tgttgggtaa
     7081 ggagtctttc gtagaggtgg gcgttttctt catcataaca gacaaagtat acctgttcgg
     7141 gtaaagcgtg acgggtaata aattctgaaa cggttttaac tgcgatttca gccgctgccg
     7201 cacgagggta accataaacc ccagtactga ttgcaggaaa agccactgac gtatagctgt
     7261 ttgccgccac cagtcgtagg ctattcagat aggcatcctg caaaagctgg tcttcgtttt
     7321 gttcaccacc acgccagact ggcccgacgg tgtgtactac ggctttagcg ggaagatcgc
     7381 ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc ctgctgttgc ctgactttta
     7441 aacaggcatc cagcagggcc ggacccgctg cgcgatgaat ggccccatcg acgccgccgc
     7501 ctcccattaa tgacggatta gccgcattca caatcacatc aacggccagt ttggtaatat
     7561 caccctgcac aacatgaata cgcgttttca taacgcctcc ttacaccttc ttgtttctta
     7621 agtgtacagc aggctttttg gttaaatcgc atttttctgc tcagcaacgt gagttgttca
     7681 gaaacttagt tatattcgat gttcattaca gccagctttt tctgtttatc aagatactgc
     7741 acttttactg aggctatttg cgcgtttttg acattgccgc tcattaacgc tttgctggag
     7801 taggttttgc ccggaataga accattctat gggcagttca tttctacggg tgatgactgg
     7861 gtgctgacgt cacatgccgg ttccacaatt tggccataaa aatggatcac gcccccttgc
     7921 gcggtgcttc ctgcaatggc aataaaagga aaaaacaaac tgccgagcat aagagagtct
     7981 aaaaaaggtc tgaacattta ttctatttcc tcaatgatta gtcatccttg agggttgtgt
     8041 tatccatact ttcaggttgg ctgattcgca gcagaccatt ctctccagat tcatcttatg
     8101 ctcgatattt caacaaatta agacttttct gaagagggcg tccattgttg tgataaatga
     8161 agtgcctgtc catcagaaac agtaacaact attttcaccc gatcttccgg ggaaatattt
     8221 aaactcaact tcgtcaaagc aatgggttga ttagcaggca atgagagggt cttttcttgc
     8281 ttcgtctgac tttgccctga actgccttcg cgcagggaca atatttgtac tctgcacaga
     8341 caagattgag taagagtaac ttcaggaata atggtgtaca tatccccttg ctgggtcgta
     8401 ttaaaggtta tctggctgga aagtgccgca aggagtaata acgcattcat attcttctcc
     8461 cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa atgatgtatt aatactgatg
     8521 agcggtcgcg ttgttaccaa agccaacctg agtcacgttg acggaggagt tcgatgcagt
     8581 ctggtcaact gcagcaccgt tgccaccacc gaactgttta acagtcattt cagaattttt
     8641 gccgttccac tgatcaagag tggcgctgtt accgaagcca cgttgggtca gatcgattga
     8701 gctgtcatct gagccctgac caacatctgc accattacca ccgccatgct gggtaatagt
     8761 caagtcagag tta
//
LOCUS       DAJTXY010000145         8711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTXY010000145 DAJTXY010000000
VERSION     DAJTXY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.145"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(13..753)
                     /gene="cysQ"
                     /locus_tag="OO566_003586"
     CDS             complement(13..753)
                     /gene="cysQ"
                     /locus_tag="OO566_003586"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6606712.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            943..2886
                     /gene="cpdB"
                     /locus_tag="OO566_003587"
     CDS             943..2886
                     /gene="cpdB"
                     /locus_tag="OO566_003587"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6606713.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3012..3392)
                     /locus_tag="OO566_003588"
     CDS             complement(3012..3392)
                     /locus_tag="OO566_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6606714.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3481..4341
                     /gene="qorB"
                     /locus_tag="OO566_003589"
     CDS             3481..4341
                     /gene="qorB"
                     /locus_tag="OO566_003589"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6606715.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            4450..5415
                     /locus_tag="OO566_003590"
     CDS             4450..5415
                     /locus_tag="OO566_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6606716.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5523..6185
                     /gene="ytfE"
                     /locus_tag="OO566_003591"
     CDS             5523..6185
                     /gene="ytfE"
                     /locus_tag="OO566_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6606717.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6230..7642)
                     /gene="cycA"
                     /locus_tag="OO566_003592"
                     /gene_synonym="dagA"
     CDS             complement(6230..7642)
                     /gene="cycA"
                     /locus_tag="OO566_003592"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6606718.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7950..8570)
                     /gene="fklB"
                     /locus_tag="OO566_003593"
     CDS             complement(7950..8570)
                     /gene="fklB"
                     /locus_tag="OO566_003593"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6606719.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALIAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         2211 a         2295 c         2207 g         1998 t
ORIGIN      
        1 tgccatctga atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg
       61 agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac
      121 agcatgtcca gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc
      181 ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc
      241 gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac
      301 cagcggcggg cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca
      361 ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa
      421 aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat
      481 aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac
      541 ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa
      601 accgtccatg ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc
      661 tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc
      721 attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg
      781 ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca
      841 tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc
      901 tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca
      961 acgctcctgg ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc
     1021 atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc
     1081 acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg
     1141 aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac
     1201 atgtcggcga aaggattaaa agcaggtgat gttcatccgg tttataaggc gctgaatacg
     1261 ctggattata cggtcggtac actcggcaat catgaattta actacggtct ggattacctg
     1321 aaaaatgcgc tggcgggagc gaaattccct tatgtaaatg ccaacgtcat tgacgccaga
     1381 accaaacagc caatgtttac accgtattta attaaagata ccgaagtggt cgataaagac
     1441 ggaaaaaaac agacgctgaa gattggctat attggcgtcg tgccgccgca aatcatgggc
     1501 tgggataaag ctaatttatc cggaaaagtg acggtgaatg atattaccga aaccgtgcgc
     1561 aaatacgtgc ctgaaatgcg cgagaaaggt gccgatgtca ttgtcgttct ggcgcattcc
     1621 gggctgtctg ccgatccgta taaagtaatg gcggaaaact cagtttatta cctcagtgaa
     1681 attccgggcg ttaacgccat tatgtttggc catgctcacg ccgttttccc gggtaaagat
     1741 tttgctgata tcgaaggggc tgatatcacc aaaggcacgc tgaatggtgt tccggcggta
     1801 atgccgggca tgtggggcga tcatcttggg gtggtcgact tacaactcag taatgacagc
     1861 ggtaaatggc aggtgacgca ggcgaaagcg gaagcacggc cgatttacga catcgccaat
     1921 aaaaaatccc tcgcggcgga agacagcaag ctggtagaaa cactcaaagc cgatcacgat
     1981 gccacacgcc agttcgtcag caagccaatc ggtaaatctg ccgacaatat gtatagctat
     2041 ctggcgctgg tgcaggacga tccgaccgtg caagtcgtga acaacgcgca aaaagcgtat
     2101 gtcgaacatt acattcaggg cgatccggat ctggcaaaac tgccggtgct ttcagctgcc
     2161 gcaccgttta aagttggtgg tcgcaaaaat gacccggcaa gctatgtgga ggtggaaaaa
     2221 ggccagttga ccttccgtaa tgccgccgat ctttatctct accccaatac gctgattgta
     2281 gtgaaagcca gcggtaaaga ggtgaaagag tggctggagt gctccgccgg acagtttaac
     2341 cagattgctc ctaacagcac gaaaccacag tcactcatca actgggatgg tttccgcact
     2401 tataactttg atgttattga tggtgtgaat tatcagattg atgttaccca acccgcccgc
     2461 tatgacggcg agtgccagat gattaatgcc aatgcagagc ggattaagaa cctgaccttt
     2521 aatggcaaac cgattgatcc gaacgctatg ttcctggttg ccaccaataa ctatcgcgct
     2581 tacggcggca aatttgccgg tacgggcgac agccatatcg cttttgcttc accggatgag
     2641 aaccgctcgg tgctggcagc gtggattgct gatgagtcga aacgtgcggg ggaaattcat
     2701 ccggcggcag ataacaactg gcgtttagca ccgatagctg ccgataagaa actggatatc
     2761 cgtttcgaaa cttccccgtc agataaagcc gcagcgttta ttaaagagaa agggcagtat
     2821 ccgatgaata aagtcgcgac cgatgatatc gggtttgcga tttatcaggt ggatttgagt
     2881 aagtaaaaca cttctttttc ggcctataaa tcaaccgcat ccggcattta ttggcggatg
     2941 cgatgctgcc gtatcttatc caccctacaa gccatgaacc gtagaccgga taagcgcagc
     3001 gcatccggca gttatgccgc acgttcatcc cgcaccgcca acacctcggg caaattcaac
     3061 tcaatccagt ccgccagtgc agcaaccttt tcgctcacct gctcgcccag cggcgtgagg
     3121 ctatattcca catgcggcgg caccaccgga tacgcgatac ggttaagaaa accatcctgt
     3181 tctaacgcct gtaacgactg cgcaagcatc ttttcactca ccccaccgat tttgcgccgc
     3241 aggtcgctaa agcgatgagt accttcgcgt agcgccacca gaatcaacac cccccaacgg
     3301 ctggtgacgt gtttcaacac ctcgcgcgac gggcactgtt ccgcaaagag attaccctct
     3361 ttcagttgtt gtgacaggct aacctgactc atttcatact tacctttttg cacgtactta
     3421 ctaaaagtaa gtttaggtgt tagcgtattt aaacacaaga caaaacgatg gagacttccc
     3481 atgatcgcta ttactggtgc cactggccaa cttggtcact atattattga atccttgatg
     3541 aaaacggttc ctgccagcca aatagtggct atcgttcgta atccggcaaa agcccaggcc
     3601 ctggcagcac aaggcattac cgtgcgtcag gctgactaca gcgatgaagc cgcactgaca
     3661 tctgcacttc agggagtgga aaaactactg ctgatctctt ccagcgaagt gggtcaacgt
     3721 gccccgcagc atcgtaatgt tattaatgcc gcaaaggcgg ctggcgtgaa atttatcgct
     3781 tataccagcc tgctacatgc agatacctcc ccgctcggcc tcgccgatga gcacatcgag
     3841 acggagaaaa tgctggctga ttctggcatc gtttacaccc tgctgcgcaa cggctggtac
     3901 agcgaaaact acctcgccag cgccccggca gcactggaac acggcgtatt tatcggtgcg
     3961 gcgggcgatg gcaaaatcgc ctcagcaacg cgggcagatt atgcgacagc tgcggcactc
     4021 gtgattagcg aagccggtca cgaaggcaag gtttacgaac tggcgggcga tagtgcctgg
     4081 acgttgacac agttagcggc agagctgacc aaacagagcg gcaaacaggt tacctatcaa
     4141 aatctgagcg aagccgattt cgccgcggca ctgaaaagcg tcggactgcc cgacggactg
     4201 gcggatatgc tggcggattc tgacgttggc gcatcgaaag gtggtctgtt tgataacagc
     4261 aaaacgctta gcaaattgat tggccgccca acgacaacgt tagccgaaag cgtaagccat
     4321 ctttttaatg ttaataacta gttaattaaa gtggcatcct cccgcatcct ctctgataat
     4381 gacgggatgc cgggagcaat catgtctgct tcctgaacgt ttcttctgac agaccaatgg
     4441 atgccagtaa tgattagcgg cgtgctgtac gccctgttag cagggttgat gtgggggctt
     4501 atttttgtcg ggccgttgat cgtgccggaa tacccggcga tgttacagtc gatggggcgt
     4561 tatctggcgt tagggttaat tgcgctaccc attgcctggc tgggacgcgt gcgtctgcgt
     4621 cagttggcgc gtcgggactg gcttaccgcc ttgatgctca ctatgatggg caacctcatt
     4681 tattacttct gccttgccag tgccattcaa cgtactggcg cgcctgtttc cacgatgatt
     4741 atcggcaccc tgccggtggt gattcctgtc tttgccaatc tgctttatag ccagcgcgac
     4801 ggcaaactcg cgtggggaaa actcgccccg gcactgattt gtattggcat cggcctggcg
     4861 agtgtgaata ttgctgagtt aaaccacgga ctccccgatt ttgactgggc acgttatacc
     4921 tctggcatcg tgctagcgtt agtttccgtg gtctgctggg catggtatgc cctgcgcaac
     4981 gcccgctggc tgcgggaaaa tcccgacaaa catccgatga tgtgggcgac ggcgcaggcg
     5041 ctggtcacgc tgccggtttc tctcatcggc tatctcgtcg cctgttactg gctgaatata
     5101 caaacgccgg acttctcctt accttttggc ccccgtccgc tggtgtttat tagtctgatg
     5161 gttgcgatag ccgtgctttg ctcatgggtt ggcgcactct gctggaacgt cgccagccag
     5221 cgattaccga cagtgattct cgggccgctg attgttttcg aaacgctggc aggtttgctg
     5281 tacacctttt tactccgcca gcaaatgccg ccgctaatga cgctgagcgg tatcgcgctg
     5341 ttagtgattg gcgtggtcat cgcggtcaga gcaaaaccag aaaaaccttt aactgaatct
     5401 gtctcagaaa gttgacatgc tggcagtgag ttaaataagc ctctgctacg taagggttat
     5461 agcttttgcc ttaaaggtgt atttaaaata catcttatct tattaagaat gaggtatcag
     5521 ctatggctta tcgcgaccaa cctttaggtg aactggcgct ctctatccct cgcgcttcgg
     5581 ctctgtttcg taaatatgat atggattact gctgtggcgg taagcagacg ctggcgcgcg
     5641 cggcggcacg taaagaactg gatgttgagg tcattgaagc tgaactggca aagcttgctg
     5701 aacaaccgat tgagaaagac tggcgtagcg ccccgctggc agaaatcatt gaccatatca
     5761 tcgtgcgcta ccacgatcgt caccgcgagc agctgccgga gctgatcctg caagcgacta
     5821 aagtcgagcg cgttcacgcc gacaaaccga gcgtgccaaa agggctgaca aaatacctga
     5881 ccatgctgca tgaagagctt tccagccaca tgatgaaaga agagcagatc ctcttcccga
     5941 tgatcaaaca aggcatgggc agccaggcaa tggggccaat cagcgtaatg gaaagcgagc
     6001 acgatgaagc gggcgaactg ctagaagtga tcaaacacac taccaataac gtcacaccgc
     6061 cgccagaagc ctgcaccacc tggaaagcga tgtataacgg cattaatgaa ctgattgatg
     6121 acctgatgga tcacatcagt ctggaaaaca atgtactgtt cccacgcgcc ctagcgggtg
     6181 agtgatctaa aagctggatg gcattgcgcc atccagcatg ataatgcggt tatttccgca
     6241 gttcagcagc ccgcttctta ccaataaaca gccagcccaa ccccagcgcg ataaaccaca
     6301 gcggggtaac cagcagcgcc tggcgagtgt catcttccag tgtcagcaac acgaccacga
     6361 acacaaagaa cgccatacat acccagcaca tcagcttgcc gagcggcatc ttgtagatcg
     6421 acttttcatg cagatgagga cgctgtttgc ggtacaccag gtacgagcaa aggataatcg
     6481 tccagacgaa cataaacaga atcgcggaaa cggttgtaat catcgtgaac gcgccaatca
     6541 cgctaggatt cacatacaac atcaccacgc cgccgagcag acagatacac gagaaggtca
     6601 accctttcgc gggtactgcg cgcttagaaa gtttagcgaa cgctttcggc gcaacacctt
     6661 cctgcgccag accaaacagc atacggctgg tagagaagac accgctgtta gcggaagacg
     6721 ccgcagaggt cagcaccaca aagttgatca cgctggcagc cgcaggcaaa cctaccaaca
     6781 caaacagctc aacgaacggg cttttctccg ggactaccga actccacggt gtcacggaca
     6841 taatcacaat cagcgcgaag acgtagaaca taatgatacg gatcggaatg gagttaatcg
     6901 cgcgtggcag tgatttctcc ggatctttgg tttccgcagc tgttgtacct accagctcaa
     6961 tccccacgaa agcgaaaacc gctatctgga atccggcaaa gaagccactt aaacctttcg
     7021 ggaaccagcc gccgtcattc cacaaatgcg cgaatgacgc ttccacaccg gtcggtgact
     7081 gaaagtgcat cgccaccatg accaggccga cgacaatcag cgacacgatg gcgacgattt
     7141 tgatcatcgc aaaccagaac tccatctcac cgaacatttt cacggtggca aggttgagcg
     7201 tcagcagcag cactatcacc gccagcgagg cgacccagtc ggagaaatcg gggaaccaga
     7261 actgagcata agccgtgatc gccaccacgt ctgccatacc ggttacaacc cagcagaacc
     7321 agtaagtcca gccggtgaaa tatcccgccc acggccccag taaatcggaa gcgaagtcac
     7381 taaaagattt gtattccaga ttcgaaagca gcaattcccc cattgcccgc atcacgaaaa
     7441 agagcataaa accaatgatc atataaacga aaatgatcga cggcccggca aggctaatcg
     7501 ttttgccgga ccccataaac aacccagtac caatggcacc gccaatggca ataagctgaa
     7561 tatgtcggtt tgtgagattg cgccgtagcg actgttcagc cggagcctga tcatcggcaa
     7621 cgacttttac ctgatctacc atgttttttc ttcctgtacc tgtctgtgtt gttcaggctc
     7681 tacggccttt agtcagtcta tgatacgacg aatctgtatc gggactcatc gatattaggt
     7741 aacaatacgc gggatgaata ctaagattta gatctaatgt taattttatg tttaaagtga
     7801 gtgtcatatc acggtgataa cgcgaaacag ctcacaaaaa tacacgctta cggggtcttt
     7861 gcaacacaaa atagcgccgt ttggcaaact gaagggttta ttgctgaatg cctgctcccc
     7921 tctggttcga ggggaacaga atgcgctgct tagaggattt ccagcagttc gacttcaaac
     7981 accagggtgc tgaacggagg gatggatgca cctgcgccgc gctcgccata tgccagttcc
     8041 tgcgggatag tcagttccca tttagaacct accggcatca gagtcagtgc ttcaatccag
     8101 ccagggatca cgccattaac cgggaattca gcgggttcac cacgagcaac ggagctgtca
     8161 aacacggtgc cgtcgatcag tttaccggtg taatgaacac gaacgcggtc ggtacgtgcc
     8221 ggaattgcgc cttcaccttg gttgatcacg cggaattgca ggccagattc ggtgctattc
     8281 acaccttctt ttttggcgtt ttcttccagg tatttcacac cttcagcagc catcgcctgg
     8341 aaacgctgac gacgaacggc atcggcgcgt tcgtggattt cacgcagcgc gcgatgcacc
     8401 acatcaaccg gcacagccgg atgtttgcct tccagcgcat cggcaatacc tgcaatcagt
     8461 gcttctggca gcagcccttc caggccagat tcactcagtt gttgccctac ctgcaaacca
     8521 atgccgtagc ttgcttgcgc ttcgatggtg tcaaaagttg gggtggtcat cgtatttcct
     8581 ttcttagggt ataaaagtag cgggcagcat atcagccaca cttctttggg taaaactttg
     8641 tcgcaggaat gatgacaaat cccggggaaa aagaaacaat agcaatatcc cgtatgaata
     8701 taataaaggc g
//
LOCUS       DAJTXY010000146         8609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTXY010000146 DAJTXY010000000
VERSION     DAJTXY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.146"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..313)
                     /locus_tag="OO566_003594"
     CDS             complement(<1..313)
                     /locus_tag="OO566_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6606720.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NF"
     gene            complement(323..871)
                     /locus_tag="OO566_003595"
     CDS             complement(323..871)
                     /locus_tag="OO566_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6606721.1"
                     /translation="MGLPPLSKTPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYTEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(871..1578)
                     /locus_tag="OO566_003596"
     CDS             complement(871..1578)
                     /locus_tag="OO566_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6606722.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1593..2270)
                     /gene="ydjY"
                     /locus_tag="OO566_003597"
     CDS             complement(1593..2270)
                     /gene="ydjY"
                     /locus_tag="OO566_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6606723.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2275..2985)
                     /locus_tag="OO566_003598"
     CDS             complement(2275..2985)
                     /locus_tag="OO566_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6606724.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3152..3958)
                     /gene="xthA"
                     /locus_tag="OO566_003599"
     CDS             complement(3152..3958)
                     /gene="xthA"
                     /locus_tag="OO566_003599"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6606725.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4404..4982
                     /locus_tag="OO566_003600"
     CDS             4404..4982
                     /locus_tag="OO566_003600"
                     /inference="COORDINATES: protein motif:HMM:NF012428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6606726.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGSIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDATCAVIVEPI"
     gene            4998..5624
                     /locus_tag="OO566_003601"
     CDS             4998..5624
                     /locus_tag="OO566_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6606727.1"
                     /translation="MPASNAFLQGLRELCDRHNALLIFDEVQTGVGRTGELYAYMHYG
                     VTPDLLTTAKALGGGFPVGALLATEECARVMTVGTHGTTYGGNPLASAVAGKVLELIN
                     TPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRGLGLLIGCVLNADYAGQAKQISLEA
                     AKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRFAAACEHFVSRGSS"
     gene            5621..7354
                     /gene="astA"
                     /locus_tag="OO566_003602"
     CDS             5621..7354
                     /gene="astA"
                     /locus_tag="OO566_003602"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6606728.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAAGWLAAGRETGQALSALEDLDGLLFTGSANTGYQLHRQLSGQPEKILALEMGGNNP
                     LIIDEVADIDAAVHLTIQSAFVTAGQRCTCARRLLLKSGAQGDAFLARLVAVSQRLTP
                     GNWDDEPQPFIGGLISEQAAQQVVTAWQQLEAMGGRTLLAPRLLQAGTSLLTPGIIEM
                     TGVGGVPDEEVFGPLLRVWRYDTFDEAIRMANNTRFGLFCGLVSPEREKFDQLLLEAR
                     AGIVNWNKPLTGAASTAPFGGIGASGNHRPSAWYAADYCAWPMASLESDSLTLPATLN
                     PGLDFSDEVVR"
     gene            7351..>8609
                     /gene="astB"
                     /locus_tag="OO566_003603"
     CDS             7351..>8609
                     /gene="astB"
                     /locus_tag="OO566_003603"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6606729.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREG"
BASE COUNT         2011 a         2215 c         2357 g         2026 t
ORIGIN      
        1 ggaagttttc gccgttcacc cagagcagag ccaccgagcc gcccgtttta cgaccggcag
       61 cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga acaatcttca
      121 ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag cggttaattg
      181 cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca tttttaattt
      241 cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc agcaaccacc
      301 cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac agcccctgag
      361 cgggaatgtc catggcagaa ttaaaacgag ccgacaggtt ttgcaggccg atcaatgccg
      421 tcagttcggt gagcgcctga gcgtcaaaat gcgcagccag tcgggtacgc agggcatcat
      481 cgactgttgg cggtgtgacg cttgcggctt cggtgtactc cagcgccagc cgttcttcat
      541 cgctaaagag cgggctttgc cgccagtcag ccactgccag cagtttatcg gtgctgccgg
      601 tacgctcggc gactttcata ctggtgatat ccacgcaaaa ctcacacagg cacatttgcg
      661 caatgcgcgc gctgacaagc gatcgcacta ccggatcgag cggtgagcgt ttgcgctcca
      721 gccacccaac aaacattgac accaaataaa agataaacgg gatccgcccc caccagcgaa
      781 tcgggcttag cacctcgccg taatggcgac gatgcagcca cgcctgcgga cgtaaaatga
      841 aaggagtttt gctaagcggc ggtaaaccca tcagtcattc ctcttctggc gctcaagcca
      901 tattttcttc gccataaaaa tcaccacggt cagggcaaac agaataaaca gttcggtgac
      961 aaaccagaaa gtaccgcctg ttaacatgct gcccgcccag gaataaacaa tagtcgccgg
     1021 taattgacca agcccggtgg cgataaaaaa cgagcgaaaa cgtattgaag tcagaccggc
     1081 agcatagctg attggatcga aagggacaaa aggcaataac cgacagacca ggatggtgtg
     1141 tttgccgtag cgagtgaaaa agccgtccat actgtcaagc acggttttgc cggttaattt
     1201 ttccaccact tcgcggccca tcactctggc gataaaaaag cacagcgccg cgccggccat
     1261 cgaactggtc cacgacagca agccgcccca gaacgcgcca aacagcgacg cattggcaaa
     1321 ggtgatcaaa aacgcaggta gcggtgcagc aatggcctgt aaaatcatca acaagaacga
     1381 gacaactgcc gccagtgcgc cgtaagactg aataaagcgt tctataccct gttggtccac
     1441 ggcggcaaac gccgcaacgc tgcgattgat aaactcatgg acgccgggaa gcagcgccca
     1501 tgcgagcagc atagcgaaca acaggatgat gagggttata cggtagtacc agattttacg
     1561 cgattgtatc ttcatcatcc tttatcctgg ctttattcgg tgattttaaa ggtaaccgtt
     1621 gccaacgtgt tatccgccgg gagaactgag gcattgcctt tgaatttcac ttcgccgcgt
     1681 ttttccaccg caccataagt gtatgttgca ttgctgacga tgccgaccgg gcagctatcc
     1741 agacacacca ggcaacccgt tttcttctct tctgctgccg ttaaattacc gccaaagcgc
     1801 atgtccagtt tcttgccatt actgtcaaca atcacttcat cgaaggaata cgcttttgcc
     1861 gccccttgcc agttgaccga aatatccagt ttgctgcctg tgacatgagt cgtttcttta
     1921 ttatccatcg tcatgttttc gcccggcgta ccacctgcct ctttcagggc ttcataaaat
     1981 gctttcgggg tcgcataagc cataaacagc gatttatgtc cgttggagcc atctttaaac
     2041 acaataccgt gacgagtgtc gtcggtgagg aaacgaccat taatttgcac cagcatggtt
     2101 accgtatggt tattggcatc gacgcgcagc ggttggctgt tcgaaagtcc gtcgtattcc
     2161 acttttgccg ccgcattctc tttttgatcg cagccgctaa gcccggcaac agccagtaag
     2221 cagagtgcag ccagtccttt tttccatgac actgaataat gttgcgacat cgttctatcc
     2281 ttcatttttt gggtgagtaa gtgggctgcg gcgcgaagca gacagatcca catgtttgtg
     2341 tcgggcgtag agttttgcga gctggatgag aataaacagc gccaggcccg ccagacagag
     2401 ttgtaaaata aagcgcagcg taatgccttc attggcgaga tcgcttgcca tcacggtata
     2461 aataacaata ccgggcaggg tcgtgagtgc cgaaataagg gtataaggcc agaaggcgat
     2521 ggtggttaat ccgtaagcgt aattttgaat attgtaagga aacaacggga ttaagcgggt
     2581 cagaataaga aaatcaatac cgttacgcgc aatgcctttt tcaatggcct ggaaggtatg
     2641 gctatggccc acgtatttca gcagtaaatc acgtcccagc cagcgcgcca gcaggaatga
     2701 gcacgaggag gccagcgttg cggcaattaa tgagagtagt gtccctaaga gcgggccaaa
     2761 aactattcct ccagcgatca ccagtatgct tcctggtaac agaaagaggg gggcattgat
     2821 gaataacaga atatagaggt tataaccgaa caatccgctc tggcgaatga gtgtctgtaa
     2881 gtggggtaaa tcggtgagca gatcgaataa accgaaagcg tggatagcgt aaatgaccag
     2941 cgcaaaaata agacaggcaa acagaaagtt acgctcagcg ttcatcgtgg agtttcctat
     3001 atcaaacttg ataatagaga agtggtcaga caactaaaaa cgcagtgacg gtataacaaa
     3061 ggacggcagg caacaaatca ttactgacag tttgttgatt tttctcaagg ttaattctcc
     3121 tgacccagtt tgagccagga gagctgctaa attagcggcg gaaggtcgcc cagacggggg
     3181 cgtgatcgga cggtttttcc atgctgcgaa tttcatagtc gatgccggtt tctacgcaac
     3241 attctgccag cggttggctg gcgagcagca ggtcgatgcg cagaccacgg ttatcgtcaa
     3301 aaccttttga gcggtaatca aaccatgaga aacgatctgc tgtttgcgga ttagcatggc
     3361 ggaaggtatc gaccaccccc cagctcatca gcctgtccat ccattcgcgc tcttccggca
     3421 ggaaagagca tttaccggta cgcagccagc gcttacggtt ttcttcgcca atgccgatat
     3481 ccagatctgt agggctgata ttcatatcgc ccataatcag taccggatta tcacgtttga
     3541 gttcggtttc caggtagttt tgcagattct gataaaactg cgcttttgcc gggaatttta
     3601 tcggatggtc gcggctttca ccctgcggga agtaaccgtt gatcacggtg acataaccca
     3661 gcagtgaggg gatttccgcc ataataatcc gccgctgcgc ctcttcgtcg tcaccgggaa
     3721 agccgcgacg cacggcaatc ggcgtctctt tggtcagcag cgccacgcca taatggcctt
     3781 tctgcccgtg ataaaacacg ttgtagccga gcttcgccac ctcttcgagc ggaaacatat
     3841 cgtcatgaac ttttgtctcc tgcaggccaa tcacatccgg ttggtgcttt tcgacgatgg
     3901 cttcaagctg gtgaggtctg gcgcgcaggc cgttgatatt aaaagagaca aatttcatag
     3961 tcgctgccat ttattcagat aaagagtgcg tggatggtag cagaatttcg cgttgcttac
     4021 cgcctgttgt taagtgattt gatcgatggt tgttaatgat gtcaacgatg gcgcaaaaaa
     4081 tgcccgcttt tggtgcgcgc tgcgtcagaa tggcgcagta atttccagta aatttcgtca
     4141 atgatcacaa ttccagaatt atattttact tctgcataac attgcgtttt ttattctttt
     4201 tattcaccca catcgcaaac gtattcactt tatatgcact ttaaatgcat atggtttggt
     4261 tataactcct tgatttccag ttagcctccg ccgtttatgc acttttatca ctggctgaca
     4321 cgaaccctgc aatctacatt tacagcgcaa acattactta ttgttaacat acaaataacg
     4381 aattatttac tgtagaggtc gctatgtctc agccaattac gcgtgaaaac tttgatgaat
     4441 ggatgatacc tgtttacgct ccggcaccct ttataccggt acgtggcgaa ggttcgcgct
     4501 tgtgggatca gcaggggaaa gagtatatcg acttcgcggg tagcattgcg gtgaacgcgc
     4561 tgggccatgc gcatccggaa ctgcgtgaag cgctgaacga acaggcgagt aagttctggc
     4621 ataccggcaa cggttacacc aacgagccgg tactgcgact ggcgaaaaaa ttgatcgacg
     4681 ccacgtttgc cgatcgcgtc ttcttttgta actccggtgc ggaagccaac gaagcggcgc
     4741 taaaactggc gcgtaaattc gctcacgacc gctacggcag ccataagagt ggcatcgtgg
     4801 cgttcaaaaa tgcgtttcat ggtcgcacgc tgtttactgt cagtgcgggt gggcagccag
     4861 cctattcaca ggattttgcg ccactgccgg cggatattcg tcatgctgca tataacgata
     4921 ttaactctgc cagcgcgctg attgacgacg ccacctgtgc ggtgattgtc gagcccatct
     4981 agggcgaagg cggtgtggtg ccagccagca acgcgttttt acaaggtctg cgtgaattgt
     5041 gtgaccgcca caatgccttg ctgatttttg atgaagtaca aaccggcgtc gggcgcactg
     5101 gtgagctgta tgcctatatg cactacggcg tgacgcccga tctgttaacc accgccaaag
     5161 cgctgggcgg cggtttcccg gtcggtgcgt tgttggcaac cgaagagtgc gcccgcgtga
     5221 tgaccgttgg cactcatggc accacctatg gcggtaatcc gctggcctcg gcggtagcag
     5281 gcaaagtgct ggagctcatc aacacaccag agatgcttaa tggcgttaaa cagcgtcacg
     5341 actggtttgt tgagcgtctt aatactatta atcaccgcta tggtttgttc agtgaagttc
     5401 gcggcttagg tttgttgatt ggctgtgtac tgaatgccga ttacgccggg caagcgaaac
     5461 agatctctct ggaagcggcg aaagcaggcg tgatggtact gattgcgggt ggcaacgtgg
     5521 tgcgttttgc gcctgcgctc aatgtcagcg aagaagaggt gacgaccgga ctggatcgct
     5581 ttgcagctgc ttgcgaacac tttgttagcc gaggttcatc atgatggtca tccgtcccgt
     5641 tgagcgctca gatgtctcgg cgctgatgca gcttgccagc aaaacgggcg gcggcctgac
     5701 gtcgcttccc gccaatgaag ccacgctttc ggcgcgtatc gaaagggcaa tcaaaacctg
     5761 gcaaggcgaa ctgcctaaaa gcgagcaggg ctatgtgttc gtgctggaag atagcgagac
     5821 aggcaccgtg gcggggattt gcgctattga ggtggcggtt gggctgaacg atccctggta
     5881 caactatcgc gtcggcacgt tggttcacgc ctcaaaagag ctgaatgtct ataacgcatt
     5941 gccgacgctg tttcttagta acgatcacac cggcagcagc gagctgtgca cgctgtttct
     6001 cgacccggac tggcgcaaag agggcaacgg ctatttgctg tcgaaatcgc gctttatgtt
     6061 tatggcggct tttcgcgaca agtttaatga caaagtggtt gctgaaatgc gcggggtgat
     6121 tgacgaacac ggctattcac cgttctggca aagcctcggt aaacgcttct tttcgatgga
     6181 ttttagccgc gccgattttc tctgcggcac cgggcaaaag gcatttattg cagaactgat
     6241 gccgaaacat ccgatctata cccacttttt atcccaggaa gcccaggacg tcatcggtca
     6301 ggtacatccg caaaccgcgc ctgcccgcgc ggtgctggag aaagaaggtt ttcgttaccg
     6361 caactatatc gacatcttcg acggcgggcc aacgcttgag tgtgacatcg accgcgtgcg
     6421 cgccatccgt aaaagtcggc tggtggaagt ggcagcaggc tggcttgccg ccgggcgtga
     6481 aacgggtcag gcgctgagtg cgctggagga tctcgacggt ttgctgttta ccggtagcgc
     6541 caataccggc taccagctgc atcgccagct ctccggtcag ccggagaaaa ttctcgccct
     6601 tgagatgggc ggtaataatc cgctaattat cgatgaggtg gcggatatcg acgcggctgt
     6661 ccatctgacc attcagtcgg cgtttgtcac agccgggcaa cgctgcacct gcgcccgccg
     6721 tttattgctc aaaagcggag cgcagggcga tgcgtttctt gctcgtctgg ttgccgtcag
     6781 ccagcgatta acgccgggca actgggatga cgaaccgcag ccgtttattg gcgggctgat
     6841 ttctgaacag gccgcacagc aggtggttac tgcctggcag caactggaag cgatgggcgg
     6901 acgaaccctg cttgcgccgc gcttattaca agcagggaca tcgttgctga caccagggat
     6961 cattgaaatg acgggcgttg gcggtgtgcc ggatgaagag gtgttcgggc cgcttttgcg
     7021 cgtctggcgt tatgatactt ttgatgaggc gattcggatg gcgaacaaca ctcgcttcgg
     7081 actcttttgc ggtctggttt cccccgagcg ggaaaaattc gatcaactgt tgctggaggc
     7141 gcgggcgggg attgttaact ggaacaaacc gcttactggt gctgccagta ccgcgccatt
     7201 cggcggcatt ggtgcttccg gtaaccatcg ccccagcgcc tggtatgccg cagattactg
     7261 cgcatggcct atggcgagcc tggagtcgga ctcgttaaca ttgcccgcca cgcttaaccc
     7321 cgggctggat ttttccgatg aggtggtgcg atgaacgcct gggaagttaa tttcgacggg
     7381 ctggtagggc tgacgcatca ttacgcgggc ctgtcgtttg gtaatgaagc ctctacccgt
     7441 caccgttttc aggtgtctaa cccgcgactg gcggcaaaac agggcttact gaaaatgaaa
     7501 gcccttgccg atgcgggatt cccccaggcc gtgatcccgc cgcacgagcg tccgtttatt
     7561 ccggtgctgc gtcagttggg attcagtggt agcgatgagc aggtactgga aaaagttgca
     7621 cgccaggcac cgcactggct ttccagcgtc agttccgctt cgccaatgtg ggtagccaat
     7681 gcggcaacga tcgcgccatc tgccgatacg ctggatggca aagtgcatct caccgttgcc
     7741 aacctgaaca ataaatttca ccgttcgctg gaagcgcccg tcactgaatc gctgttaaaa
     7801 gcgattttta aggacgaaga gaaatttagc gtccattcgg cgttgccgca ggtagcgttg
     7861 ctcggtgatg agggggcggc aaaccacaat cgtctcggcg gtcattacgg tgaaccgggt
     7921 atgctacttt ttgtctacgg gcgagaagaa ggcaatgata cccggccttc ccgttatccg
     7981 gcgcgacaga ctcgcgaagc cagcgaggcg gtggcaaggc ttaatcaggt gaatccccaa
     8041 caggtgattt tcgcccagca aaacccggac gttatcgacc agagcgtttt tcataatgac
     8101 gtgattgccg tgagtaaccg ccaggtgctg ttttgccacc aacaggcgtt cgctcgccag
     8161 tcacagttac tggcaaacct gcgggcgcgg gtgaacggtt ttatggcgat agaagttccg
     8221 gcaactcagg tttccgtgtc ggatgcggtg tctacctatc tgtttaacag ccaactgttg
     8281 agccgcgatg atggttccat gatgttggtg ctgcctcagg agtgtcggga acacgccgga
     8341 gtatggggtt atctcaatga actccttgcc gctgacaacc cgattagcga actaaaagtc
     8401 tttgacttac gtgaaagcat ggcgaatggc ggcggcccgg cgtgcctgcg gttgcgggtg
     8461 gtattgacag aagaagaacg ccgggcggtg aatccggcgg tgatgatgaa cgataccttg
     8521 tttaatgcgc tcaatgactg ggtggatcgt tactaccgcg atcgtcttac tgctgccgat
     8581 ctggccgacc cgcaattgct gcgcgaagg
//
LOCUS       DAJTXY010000147         8575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTXY010000147 DAJTXY010000000
VERSION     DAJTXY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.147"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="OO566_003604"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="OO566_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6606730.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="OO566_003605"
     CDS             962..1303
                     /locus_tag="OO566_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6606731.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="OO566_003606"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="OO566_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="OO566_003607"
     CDS             complement(1901..2611)
                     /locus_tag="OO566_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6606732.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="OO566_003608"
     CDS             2719..3024
                     /locus_tag="OO566_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6606733.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..3546
                     /locus_tag="OO566_003609"
                     /pseudo
     CDS             3223..3546
                     /locus_tag="OO566_003609"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4102..4701
                     /locus_tag="OO566_003610"
                     /pseudo
     CDS             4102..4701
                     /locus_tag="OO566_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(5293..6282)
                     /locus_tag="OO566_003611"
     CDS             complement(5293..6282)
                     /locus_tag="OO566_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606734.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA"
     gene            complement(6357..6866)
                     /locus_tag="OO566_003612"
     CDS             complement(6357..6866)
                     /locus_tag="OO566_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606735.1"
                     /translation="MSSKASALFNNNDWKLHVTDINLYENTVSLDGQSYPLSFAIKTL
                     IPGYLSGLPATSRESMELLEALAEAGVTIGNFFSNDLMTAYQRRQQNKRAEAERIAKE
                     QRIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADIE
                     SGGNWMDSL"
     gene            complement(6968..7189)
                     /locus_tag="OO566_003613"
     CDS             complement(6968..7189)
                     /locus_tag="OO566_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606736.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRRLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
     gene            complement(7436..8281)
                     /locus_tag="OO566_003614"
     CDS             complement(7436..8281)
                     /locus_tag="OO566_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606737.1"
                     /translation="MNTNEVSVINLDQLVSMSSYDFLTKVINPARVEAGEKPVRNNDF
                     VSRIEDELDGQNVAYEIFVSFGQDVKSYTLNTDQLLLVGMRESKAVRRKVLAYIRQKE
                     QEVLELQAQQLQVAQTKAICYAPGAIVLDSKLDSKSGATPTEVIKALDLKLTSSKMLT
                     ASVLTGYLKTLCRPNPFYTKRVTEFGTTDIDLSTGRPRAIFNKKGETNGYYRLQPQPN
                     DKEPTIKILRKGYDWLKDNWKDIAHDMRTSNIKVVRRLVDSHLNGKATKSQKAAAGKG
                     SDYGF"
BASE COUNT         2146 a         2149 c         2150 g         2130 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg
     3301 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     3361 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     3421 cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat
     3481 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     3541 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     3601 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     3661 gtcgaacagt atggcaatga agctgtatat attcagtacc cttcggggat tgttggcggc
     3721 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     3781 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     3841 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     3901 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     3961 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4021 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4081 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4141 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4201 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4261 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     4321 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     4381 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     4441 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     4501 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     4561 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     4621 ttcatctgga actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt
     4681 aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc
     4741 attagacggg atgtctagac ggctggtcag gcccccggtg gcctctttgt caggactcag
     4801 ggtggcaccg gggaacattg gtgtcttaat tgtattgaat gaggtctcta acttttggtc
     4861 caaaactcat cgttagctgc tcgccagctc acagccgaac ccgaactgta agcacgacca
     4921 ccagcaccag cttcagcagg aagacgacaa gatagacttt atgcaggtcc atgcgtcctc
     4981 ctgtgtctat tgtaccactg cactagataa ctagtacaaa ataaagtcca gaccgaaggc
     5041 ccagaccata gttaattaat cacattgata atccaccacc tgaagtcctg ccaacattca
     5101 gtccagcctt acccatgcta ggagtcttaa ccggggtgtc gttctgtgtc tccaccactg
     5161 gtgccgcatc aggctctaca ggaagctcca cagcaggcgc agggaccgga ggtgatacgg
     5221 atgtaccacc accagcggag aagcccttag cgatggaccg gaggccttta ctaatgctct
     5281 tacccattgc tgttacgctt cggtcttggt gaacttcagg cgtttgacta caggcaggac
     5341 tgcggttgca gcgccagtgt ccagcttaac tgtcgtagca gcttcagcga caacgaagac
     5401 cttaccgagg atttccaagt cgtgggactg agagtcgcca tcacgtgaaa ggacgaccgt
     5461 aatattctca ccgtcgtgac ggaagcagcc gaagctcagg gccgtgaagc gtttagccag
     5521 catgaacaca cctttgtcct caccgttgta atacgcgatg tcggtaccca gcatatccac
     5581 gatagcgtct tcaccagaga agccctgttt agcagccaga cgggacagcg ctttcagttg
     5641 ggactcatgg gcgatgatgc cgtaatcaga cacttcagag ccgaaagagg actcggtgtt
     5701 ctcaaacaga gcgtccatga tagcgtctgc cttagtggtc aggcttccag tcaggactgg
     5761 cagctctacg gtcttcagca gcttggagga ctccagaacg tcaaccatgc gagcgttgat
     5821 ggtacggacc acattctcac agatgttcat agcgtcctgc tggagtgtgc catgagtgaa
     5881 accagtggag gacttggcgt cccacagttt agtttgctcc gagatgagga ttgcctgttc
     5941 gataggttcg acgacaatct cctgaccaga cggaacggtc acgacttcag gtcgctcacc
     6001 accgcgtaca gggacgacca cagagcggtc agacagggtg tgggcgatga ttggggtagg
     6061 caggctattg cgggtaggca ggacttctac agggaacgga atgttatcct gagcgtcttt
     6121 aagtacaccg ttcagcatag ccttaaccgt cttagctaac tggagggcat agacctgtgg
     6181 gtcagcgtta gttggcagac cttcgttaac ctgaatgttg tcggtgtcgg tgatacgtac
     6241 tggctcaatg ccgaactgat agctatcttt aatcatggtc ataataatca atctcctaaa
     6301 ttagttttag atactgtggg tcccgacatc aggtcaggac cacttgtgtg tctgtcttag
     6361 aggctgtcca tccagttacc gcctgactca atgtcagcga caatagcggc acgggagcga
     6421 ccagcggaat gggtagcact gcggaggttc tcaccatagg tccggcgctc agctttgacc
     6481 tgttcgcggc gctgtagctc tttctgccac tctgcatcgg tcatgttctc ttcacgcata
     6541 cgctcagcct gaatgcgctg ctctttagcg atacgctcag cctcggctcg cttgttctgc
     6601 tggcgacgtt ggtaggcggt catcaggtca ttgctgaaga agtttccaat ggtcacgcca
     6661 gcttcagcca gagcttccag taattccatg gactcgcggc tggtggcagg cagtcctgac
     6721 aggtagcccg gaatcagggt cttaatagcg aaggacagtg ggtaggactg accgtccaga
     6781 ctcacagtgt tttcgtagag attgatgtcg gtgacatgga gtttccagtc attgttgttg
     6841 aataacgcac tagctttgct agacatagta attactcctc ttgagagttg agactatcgg
     6901 taccgcagac acagttatgc ctacgggtct ccccgcatcg gtcatctcaa aaggtttaaa
     6961 ttaggggtta gtgagtggcg tccagcttag cgaacgctcg gcgtaactga ttgttcaggt
     7021 cacggtcgac cagcaggtct atcagttcgt ctcgactata gaaaggacgc tcagggtcct
     7081 ctacgcccat ccccagcatt gacaggaggc gacgggtagg cagaccacgg taacggagag
     7141 tagcctttcc gatgggtgtc cgtgggtcga tatgttgttt agttgtcatt gttagaagtc
     7201 ctcgttagac caatcatcca cgtcaaattc actggtgata ggagcttgga agttaggtag
     7261 accctctggt atctctagag gcagtcgctc gtcgactctc agtgccatct cttcgtgtat
     7321 atgccgaagc agagaagtca gtaagatagg gtccgcagga tgctcagggc cgtgtctctc
     7381 aatgaaacat atggttgtcg ctattgactc cataatcagt tcatcagtga gtcgcttaga
     7441 acccatagtc agaccccttg cccgctgctg ctttctggga ctttgtagcc ttaccattca
     7501 ggtgagaatc caccagacga cggacaacct tgatattgga tgtgcgcatg tcatgagcga
     7561 tatccttcca gttatccttc agccagtcat aacccttgcg tagaatcttg atggtaggct
     7621 ctttgtcgtt aggctgtggc tgtagacgat agtagccgtt ggtctcacct ttcttattga
     7681 agatggctcg tggacgtccg gtcgataggt ctatatccgt agtgccaaac tcagtaacac
     7741 gcttggtgta gaatgggtta ggacgacaca gggtcttcag atatccagtc aggacgctag
     7801 ctgtcagcat cttagagctg gtcaacttaa ggtccagagc tttaataacc tcggtaggcg
     7861 tggcgcctga cttggagtcc agcttggagt cgaggacgat agcaccgggc gcatagcaga
     7921 tagctttggt ctgtgcgacc tgaagttgct gggcctgaag ttcgaggacc tcttgctcct
     7981 tctggcgaat ataggctagg accttacgac gcacagcttt agactcacgc atcccgacca
     8041 gcagaagctg gtcagtgttg agggtatagc ttttaacgtc ctgcccgaag cttacgaaaa
     8101 tttcgtaggc tacattttga ccgtccagct catcttcaat acgggacaca aagtcgttgt
     8161 tacggacagg cttctcccca gcctccacac gagccgggtt gatgacctta gtcaagaaat
     8221 cgtagctact catagacaca agctggtcaa ggttgatgac gctaacttcg ttggtattca
     8281 taaaggatgc tcctttggta aagcactttc tgtctccatg tggagtccgc acctattcaa
     8341 tgcatccaaa cggacccacc aaaaagtgct taacgaaagt gaatagggtt aatgttgatt
     8401 agtgttggtc tggagtgacc atgttctcca tgtgcatata aggcgagacc gcatgtcagg
     8461 acgagctgta aggaccccag atggtgagcg gtgatagatg aagtggtcaa caaaaactgg
     8521 ccaccgagtt agagtttttc cagtatcgat tttccgattc gtttgggggt aaccc
//
LOCUS       DAJTXY010000148         8448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTXY010000148 DAJTXY010000000
VERSION     DAJTXY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.148"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..657)
                     /gene="istA"
                     /locus_tag="OO566_003615"
     CDS             complement(<1..657)
                     /gene="istA"
                     /locus_tag="OO566_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR6606738.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHHNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     P"
     gene            complement(744..1105)
                     /locus_tag="OO566_003616"
                     /pseudo
     CDS             complement(744..1105)
                     /locus_tag="OO566_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1445..1999)
                     /locus_tag="OO566_003617"
                     /pseudo
     CDS             complement(1445..1999)
                     /locus_tag="OO566_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            2142..2264
                     /locus_tag="OO566_003618"
                     /pseudo
     CDS             2142..2264
                     /locus_tag="OO566_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2526..3092)
                     /gene="ipgB2"
                     /locus_tag="OO566_003619"
     CDS             complement(2526..3092)
                     /gene="ipgB2"
                     /locus_tag="OO566_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6606739.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3374..3688)
                     /locus_tag="OO566_003620"
     CDS             complement(3374..3688)
                     /locus_tag="OO566_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606740.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3766..3876)
                     /locus_tag="OO566_003621"
                     /pseudo
     CDS             complement(3766..3876)
                     /locus_tag="OO566_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4200..4877)
                     /gene="ospD1"
                     /locus_tag="OO566_003622"
     CDS             complement(4200..4877)
                     /gene="ospD1"
                     /locus_tag="OO566_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6606741.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(5248..5385)
                     /locus_tag="OO566_003623"
                     /pseudo
     CDS             complement(5248..5385)
                     /locus_tag="OO566_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5467..5765)
                     /locus_tag="OO566_003624"
                     /pseudo
     CDS             complement(5467..5765)
                     /locus_tag="OO566_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6354..6513)
                     /locus_tag="OO566_003625"
                     /pseudo
     CDS             complement(6354..6513)
                     /locus_tag="OO566_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6517..7746)
                     /locus_tag="OO566_003626"
                     /pseudo
     CDS             complement(6517..7746)
                     /locus_tag="OO566_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7766..8113)
                     /gene="tnpB"
                     /locus_tag="OO566_003627"
     CDS             complement(7766..8113)
                     /gene="tnpB"
                     /locus_tag="OO566_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6606742.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(8113..>8448)
                     /locus_tag="OO566_003628"
     CDS             complement(8113..>8448)
                     /locus_tag="OO566_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6606743.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQIL
                     IREIKGSSH"
BASE COUNT         2165 a         1985 c         1876 g         2422 t
ORIGIN      
        1 gggagagaag cccatctcct tgccgaactg ccacagcgaa ggatggaacc ggtgctgacc
       61 ggtctgatat gcgtcacgtt gcagaaccac agttttcata ttgtcataca acacttcgcg
      121 cggcacacca ccaaagaagc ggaacgcatt atgatggcag gtctccagcg tgtcataacg
      181 catattgtca gtgaattcga tgtacaacat tcggctgtat ccgggaacag caacgaacac
      241 gtgaagcggt gagcgaccat tacgcatagt gccccagtca acctgcatct gtcgtccggg
      301 ttcagtttcg aaccgaacgg caggctcctg ctcctgagga accgagagag aacgaatgaa
      361 tgccctgaga atggtcattc cgccacgata tccctggtct ctgatctcgc gagcgattac
      421 cgttgccggg attttgtaag gatgagcatc ggcgatgcgt tgacgaatat aatcccggta
      481 ttcatccagg agtgaagcaa cagcaggtcg cggcgtatat tttggcggct cagattttgc
      541 ctgcaaataa cgtttaacgg tattgcggga gagccccagt tctctggcaa tcgcccggct
      601 actcattccc tgcttgtgca ggattttaat ttccataact gtctcaaaag tgaccataag
      661 ctctcctgaa tcaggagagc agattacccc ctggatctga tttcaggcgt tgggtgtgga
      721 tcactattgc accgttcatg acagtgttgt tgcgccttgc tgccaacgaa gctccactgc
      781 ccatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
      841 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agcgtacggg
      901 cagtatcgcg aactccggca ccgttgaaag ccatttcagt gatttggttt ttaactcccg
      961 gtttacgggc ctcgtaacag taagtgagct gaaacacccg gtgacagtca cggcaacgga
     1021 atcgatcatg tccttttggg ttctgcccgt gacggtaaac ctgaacggac tggcaacggg
     1081 ggcaatgaaa gttaactgtg accatgaaag aaccttaaaa gcgggtatta taacccctaa
     1141 ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa atcctctcac
     1201 cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat aatgatatga
     1261 cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac agtgcctgtg
     1321 gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca gcatctgaaa
     1381 acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta atatggacac
     1441 aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca caccaactgt
     1501 gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc cgcattattt
     1561 cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga aagaagcttt
     1621 ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc agattatgcc
     1681 acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc gagtgaacga
     1741 taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg caggccagtt
     1801 gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac aggtcaatga
     1861 ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct cccgcccact
     1921 tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac acaggcaggc
     1981 catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact ggtatttact
     2041 acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt acggttccgt
     2101 aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc gacagcggtg
     2161 acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac aatgtggagc
     2221 cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca aaccgggtac
     2281 gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata tgattctgac
     2341 gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg agctaggaaa
     2401 atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga ggagaaatat
     2461 agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact cgggatgttc
     2521 tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc acgtaatttc
     2581 tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag atttgcaaca
     2641 ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc gtccattttc
     2701 tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc agcattgatg
     2761 tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg gagattatcg
     2821 ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggttcac cacccgggat
     2881 acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat ttgttctgaa
     2941 acagagctca aaatactagt atttattttg gttgatgata tttttactat ccttttcccc
     3001 attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg agataaactg
     3061 attccaaaat tattaaaaga tgttccaagc attataatca ctttgtcatg taaaatagta
     3121 acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt tctttactcc
     3181 cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag aaagagagta
     3241 tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat aaccggcatc
     3301 attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa aattgctgtt
     3361 cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac atcactggct
     3421 tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc cgttttgtat
     3481 aaacggtcac ggtatgcccg gagccactgt tgctgatgaa ttcgataaaa ccttggcgtt
     3541 tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc gctcttttgc
     3601 cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc tgagcaaacc
     3661 caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta gccgctgaca
     3721 gcttctataa aagtatgatc ctacccacgt aatatgaaca caggcttaag tgaggttctg
     3781 gttttcaaat tgttcaggac tgagacctcc acaccaactg tgccgtcgtc agcgattgta
     3841 atcgcacttg atataattaa acactgtata tttcatttgt ggagtctcca gtctgagggg
     3901 gctttatgca ttccgtattg ggcactatga tgccagaaat ctgcaaggct ccacaccttc
     3961 ctgtatttcc cttcctctat cacagtgcac tttacttaat agggtgggat gtgttcatta
     4021 catttattgt gttttttatg attgtttttg cgtccagtgt cgttcgtcat gggaaactgg
     4081 ttctatgggt agtatcgctc agtctagtta actgtttgtt tctttttaga aattaagcag
     4141 atcacacaat tcggggagag atccttctaa gtgcaattct ttgcagctat ttagcacact
     4201 taaaacaact cttgtatttt gtcagaaata atattaaaca tgtcttgttc attattgtta
     4261 agtctgtctg ctatttctgt aagttttatc ccactatgat ggcatatatt tatatatgcc
     4321 atcatagcct cgatattccc tttccctgcg gccagaaata atcctggtaa cccctcatta
     4381 tctcttacat ccaatagttt aatcgtttct tttggtgata aatcaaaagt tttaataaaa
     4441 tctccataag cttgaatcgc agcggaatga ccttcttgaa gagctatgaa caaagcagat
     4501 tccccagcat tatttcgtgc tgcaagaact tctagttttt cctctttggg tatttcggct
     4561 gccaacagcg attcaccgta tttacgcgct gcatcggcat ttccatgcca aatcaatgaa
     4621 aacaaattat ggcggttata ctccttatta ttctttgtcg gaatggatat gctctgtatt
     4681 ttggccaatt ttctgaaaga aacaccacta tatccccccc ctgatttttc ttcattgatg
     4741 gcgtgggata tagcggtatt tgtcacgtta atgatattat ttttcattcc tttattttca
     4801 ttagcagtat tgctgcctat tattaggtgc atatttttgt tgtttgctgg atgtaatcca
     4861 tagttattta ttgacatttt tgttcatcct ttttgctaaa aaaaacaata acctattttt
     4921 gtaatgtaat agatattagt ctattgctaa tctaaaaaaa ccattgccac attgaatggc
     4981 tatattcacc aataaattct ttcgatttcg tatattgaca tatcgtagat atatcttatc
     5041 cctaagattc aataatcgag tccgtttacc attctgtgca agcgccttca ctcagaagtg
     5101 accgtcaagg cgatcaccaa acttgataat aaagcagctc attgccatac gccagtcctt
     5161 aaggggcgtt gtccatttct gtgatgctgc ctagatttaa ctgccagctc cggcttatct
     5221 acatctaaat atgtgagcct aataccgcta cactttgtcc agactatgtt tttctccggg
     5281 aagtgggcta accgtctcca taaagtgacg aacttttttc aacagtggcc atattgaacg
     5341 gcactgatga ttacgggtta ttgtctcatg aagagccggc cacatcactg acagcagtga
     5401 cttcaggcct tcagcaccag acagcctgaa ccagttaatc catcgcccga cagatttagt
     5461 aatctagggc tggtttttct tcagctgttg cggttcgaaa gtgccattac gatcacgcag
     5521 agtacggatt tccagaggtt catctccagt agtgataatc ttcgttaagc aaccattgcg
     5581 ggaattgctg ccagatctaa gctgattttt ctcgcaccca agatgatgag tcatattagc
     5641 attgagtacg gtttcatttt cagcatccaa tcaaactgac tgagatcttc aggatttttg
     5701 agacttttgg ctagttcatt agctaaagcc cgcaacttat tagatgtcaa ctgtttttca
     5761 ttcataaatt caccttcatt tgatatcaaa ttaaatagtt caacatcaga gaattataca
     5821 aacttatgta caggctccta tgatcttcga atcctgaaga ttatagtgaa aataagtttt
     5881 cacgttgaaa tcccacatag ttagtatatg ataaataatc atggatgacc tgattaacct
     5941 cttggggtag tgcttttata gaagaataaa tattctacta atttaataca tcacctaaaa
     6001 tagtattgtt ttttatggtt ggttgaaggt cgtggtgatg atgttagtcc ttttgagaat
     6061 attccagtgc cagatcttac ttataataaa ggggcttggc agtttggata aactattgtt
     6121 cttattccac taatacaaac tttttgtaaa gtatattaaa tatacttcat ttcatactga
     6181 aatccccgga agtattcaat caaacaatta caccataaaa ggaagagggg gccctaatta
     6241 agttgtcaag catgtttatg atacctgcag ggtataaatt ttctggctgc aagcagacac
     6301 atataaaacg tctttattga tcctacccac ataatatgga cacagtcgta agtgaggctc
     6361 tggttttcaa attgttccgg actgagacca ccacaccaac ggtgccgtcg ccagcgattg
     6421 taatcgcact cgatataact aaacactgtt gcccgcacta tttcccgatg gataatagtg
     6481 ttctccgtgg agacattcca ttttcagcga atgaattttc atctgttgct gtatccagtc
     6541 atacagtgac tgcatcagtg gcgcggctct ggcttttctt gccgccagac gctgttctgc
     6601 tgaacatccc cggacttctg cctcgatggc atacagttca ccgatacgct gcagggcttc
     6661 cgtggtgatg tcggttggca ctcttgcatg cacatcgtgg atttttctcc ggacatgagc
     6721 catacatgcg gcttccgtta ttctgccgga ttcgtacaac gcccggtaac caccgtaagc
     6781 atcggcctga agcactccgc tgtaaccggc cagatgattc tgtggatgga tacctttccg
     6841 gtccggtgag tacgcgaacc agaccgccgg aggcatttcc gaaccggcgt tacggtcatc
     6901 acgaacgtag acccacagcc gggcagtcct tgttttaccg ctgcccggct cctggaccgg
     6961 aacggggata tcatcggcat ggactttacc gggcatcagc acatactggc gcaggatgtc
     7021 atacagcggt tccagcagtt cagcaacagc acctgtccag cgccccagtg tggcacggct
     7081 cagctccacg ccctgacgac ggtatatttc tgactggcgg tataacggca gatagtctgc
     7141 atattttcca gcgacaacat gggccagaag ccccgctccg gcataactgc gtgcaatggg
     7201 ttttgaaggc accggtgcct gcacgatatg gtcacaccgg caacaggcca gtttcggacg
     7261 ttgtgtttcg ataaccttaa aggcgctgct gataagctcc agctgctctg acacatcaca
     7321 tcccagagaa ctgagttcgc caccacaggc cgggcagcat tcctcttccg gacggatgac
     7381 ccgggtttca cggggaagtg aggcaggtaa cggtttacgg gctgaagact ggcgcagggg
     7441 agatggcagt atcgggtcat attgctcacc cagcgtttcc gccatttctt cctgaagtgc
     7501 gctgattcgc tcctgtgctt ccagtatctg ccgttcggtt tttgcgcgaa gtttttctga
     7561 gcttttaccg aactgcatgc gctgcagttt cgcaaccagc gccttcagcc ggttgatttc
     7621 ggaagcataa gccgccaccc gctgtgagag caggcggttg tatttcgcca tctggcggat
     7681 ggtgtcctgt tgcgtctgca acagtgcccg caggcgggca ttctcatgag aaagtgaggt
     7741 gtccatatcc tcactttaca acgggttata tgcggatgcc agcgcgttcc gttcgtttcg
     7801 ggtgtttcca gtcgatgccc tcaagaagca tggataactg ggccggagta aggtgcacct
     7861 tgccgtcacg ggtgaccggc cagacgaagc ggccccgctc caggcgtctg gtaaagaggc
     7921 acagtccgtc actgtcagcc cacaacactt ttatctggtc accccggcgt ccgcggaaga
     7981 tgaacagatg cccggagaac gggtcatcct tcaggacgtt ctgaactttt gaggccaggc
     8041 cgttaaagcc atttcgcata tcggtgatac ctgcaaccag ccagatacgc gaacctgcag
     8101 ggaaagatat catcagtggc tgctcccttt tatttcgcgg ataagtatct gtaataacgc
     8161 cggcgtcagt ttacctttaa gcctgagagt tccggccggc agaaccagct cacagcacag
     8221 actgtcggac ggtgtattta tctgctccgg cgttaacgtc acgggaagca gtgcaggcat
     8281 attttttccg gaaggcagca ggccaccttt ccggtattga tggcgccagt tgaagagcag
     8341 gttatcgttg attccgtttt cacgggcgat ctgcgccaca caggctccgg gctgcagtga
     8401 ctgctccact aaagcgattt taaattcata aggaaaatta ggccgccg
//
LOCUS       DAJTXY010000149         8391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTXY010000149 DAJTXY010000000
VERSION     DAJTXY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.149"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(127..918)
                     /gene="aadA1"
                     /locus_tag="OO566_003629"
     CDS             complement(127..918)
                     /gene="aadA1"
                     /locus_tag="OO566_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6606744.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(1011..1484)
                     /locus_tag="OO566_003630"
     CDS             complement(1011..1484)
                     /locus_tag="OO566_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6606745.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1644..2657
                     /gene="intI1"
                     /locus_tag="OO566_003631"
     CDS             1644..2657
                     /gene="intI1"
                     /locus_tag="OO566_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6606746.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2860..3210
                     /locus_tag="OO566_003632"
     CDS             2860..3210
                     /locus_tag="OO566_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6606747.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3336..3896
                     /locus_tag="OO566_003633"
     CDS             3336..3896
                     /locus_tag="OO566_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6606748.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3899..6865
                     /locus_tag="OO566_003634"
     CDS             3899..6865
                     /locus_tag="OO566_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6606749.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6932..7309
                     /locus_tag="OO566_003635"
     CDS             6932..7309
                     /locus_tag="OO566_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6606750.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7510..8169)
                     /locus_tag="OO566_003636"
     CDS             complement(7510..8169)
                     /locus_tag="OO566_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6606751.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1860 a         2447 c         2276 g         1808 t
ORIGIN      
        1 tcaaaaacac catcatacac taaatcagta agttggcagc atcaccgcaa ttatgtgctt
       61 agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg aacgaattgt
      121 tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgaa caaattcttc
      181 caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc
      241 ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac
      301 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
      361 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
      421 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
      481 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
      541 cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag
      601 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
      661 tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
      721 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
      781 actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
      841 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
      901 gatcaccgct tccctcatga tgtttaacgc ctggcacagc ggatcgcaaa cctggcgcgg
      961 cttttggtac aaaaggcgtg acaggtttgc gaatccgttg ctgccacttg ttaacccttt
     1021 tgccagattt ggtaactata atttatgtta gaggcgaagt cttgggtaaa aactggccta
     1081 aaattgctgg ggatttcagg aaagtaaaca tcaccttccg gctcgatgtc tattgtagat
     1141 atatgtagtg tatctacttg atcgatcagg cttttgtata tctccccacc acctgaaaca
     1201 atgacatgat ccgttatttt ctttaggttg gttaaagcat ctttaattga tggaaagatc
     1261 actacgttct cattgtcaga tgtaaaactt gaacgtgtta cgaccgcata ctttcggttg
     1321 ggtaatgctc ccattgattc aaaagtcttg cgtccaacca acagccattg gttataggta
     1381 atagctttaa acaggagctg ttcacctttg gcactccatg gaatatcagg gccattcccg
     1441 ataactccat tcttcgatat agctaccatt agtgatagtt tcacaattct tcctcagagg
     1501 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
     1561 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
     1621 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
     1681 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa
     1741 ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc
     1801 cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca
     1861 aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg
     1921 tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct
     1981 tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc
     2041 atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg gatcagtgag ggtttgcaac
     2101 tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct
     2161 ccaaggatcg ggccttgatg ttacccgaga gcttggcacc cagcctgcgc gagcagctgt
     2221 cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg ccgcagcggc gttgcgcttc
     2281 ccgacgccct tgagcggaag tatccgcgcg ccgggcattc ctggccgtgg ttctgggttt
     2341 ttgcgcagca cacgcattcg accgatccac ggagcggtgt cgtgcgtcgc catcacatgt
     2401 atgaccagac ctttcagcgc gccttcaaac gtgccgtaga acaagcaggc atcacgaagc
     2461 ccgccacacc gcacaccctc cgccactcgt tcgcgacggc cttgctccgc agcggttacg
     2521 acattcgaac cgtgcaggat ctgctcggcc attccgacgt ctctacgacg atgatttaca
     2581 cgcatgtgct gaaagttggc ggtgccggag tgcgctcacc gcttgatgcg ctgccgcccc
     2641 tcactagtga gaggtagggc agcgcaagtc aatcctggcg gattcactac ccctgcgcga
     2701 aggccatcgg tgccgcatcg aacggccggt tgcggaaagt cctccctgcg tccgctgatg
     2761 gccggcagca gcccgtcgtt gcctgatgga tccaacccct ccgctgctat agtgcagtcg
     2821 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacaa tggaggtggt agccgagggt
     2881 gtggaaacac ccgactgcct tgcgtggttg cggcaggcgg gttgcgacac ggtgcagggt
     2941 ttcctgttcg ccaggccgat gccggcggcg gccttcgtcg gcttcgtcaa ccaatggagg
     3001 aacaccacca tgaacgccaa tgaaccgagc accagttgct gcgtgtgctg caaggaaatc
     3061 ccgctcgatg ccgccttcac gccggaaggg gccgagtacg tggagcattt ctgcgggctg
     3121 gagtgctatc agcgcttcca ggcgcgggcc agcactgcga ccgaaaccag cgtcaaaccg
     3181 gacgcttgtg attcgccgcc gtcaggttga ggcataccct aacctgatgt cagatgccat
     3241 gtgtaaattg cgtcaggata ggattgaatt ttgaatttat tgacatatct cgttgaaggt
     3301 catagagtct tccctgacat tttgcaggga attccatgac tggacagcgc attgggtata
     3361 tcagggtcag caccttcgac cagaacccgg aacggcaact ggaaggcgtc aaggttgatc
     3421 gcgcttttag cgacaaggca tccggcaagg atgtcaagcg tccgcaactg gaagcgctga
     3481 taagcttcgc ccgcaccggc gacaccgtgg tggtgcatag catggatcgc ctggcgcgca
     3541 atctcgatga tttgcgccgg atcgtgcaaa cgctgacaca acgcggcgtg catatcgaat
     3601 tcgtcaagga acacctcagt tttactggcg aagactctcc gatggcgaac ctgatgctct
     3661 cggtgatggg cgcgttcgcc gagttcgagc gcgccctgat ccgcgagcgt cagcgcgagg
     3721 gtattgcgct cgccaagcaa cgcggggctt accgtggcag gaagaaatcc ctgtcgtctg
     3781 agcgtattgc cgaactgcgc caacgtgtcg aggctggcga gcaaaagacc aagcttgctc
     3841 gtgaattcgg aatcagtcgc gaaaccctgt atcaatactt gagaacggat cagtaaatat
     3901 gccacgtcgt tccatcctgt ccgccgccga gcgggaaagc ctgctggcgt tgccggactc
     3961 caaggacgac ctgatccgac attacacatt caacgatacc gacctctcga tcatccgaca
     4021 gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag ctctgttacc tgcgctttcc
     4081 cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc ttgttgaagc tggtcgccga
     4141 ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc cagcgggagc agacccggcg
     4201 cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg cccttcacca tgagccatta
     4261 ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa accgacaaag gcatcgtgct
     4321 ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc attctgcccg ccctcaacgc
     4381 cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac cggcgcatct acgacgcctt
     4441 ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac gatctgctca agcgccggga
     4501 caacggcaag acgacctggt tggcttggtt gcgccagtct ccggccaagc caaattcgcg
     4561 gcatatgctg gaacacatcg aacgcctcaa ggcatggcag gcactcgatc tgcctaccgg
     4621 catcgagcgg ctggttcacc agaaccgcct gctcaagatt gcccgcgagg gcggccagat
     4681 gacacccgcc gacctggcca aattcgagcc gcaacggcgc tacgccactc tcgtggcgct
     4741 ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc gacctgcacg accgcatcct
     4801 gggtaagctg tttaacgctg ccaagaataa gcatcagcag cagttccagg cgtcaggcaa
     4861 ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt caggcgctga tcgacgccaa
     4921 gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc atgtcctggg attcctttgc
     4981 cgagagcgtc accgaggcgc agaagctcgc gcaacccgat gacttcgatt tcctgcatcg
     5041 catcggcgag agctacgcca ccctgcgccg ctatgcaccg gaattccttg ccgtgctcaa
     5101 gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc attgaggtgc tgcgcggcat
     5161 gaacaccgac aacgcccgca agctgccagc cgatgcaccg accggcttca tcaagccgcg
     5221 ctggcagaaa ctggtgatga ccgacgccgg catcgaccgg cgctactacg aactgtgcgc
     5281 gctgtccgag ttgaagaact ccctgcgctc gggcgacatc tgggtgcagg gttcacgcca
     5341 gttcaaggac ttcgaggact acctggtacc gcccgagaag ttcaccagcc tcaagcagtc
     5401 cagcgaattg ccgctggccg tggccaccga ctgcgaacaa tatctgcatg agcggctgac
     5461 gctgctggaa gcacaacttg ccaccgtcaa ccgcatggcg gcagccaacg acctgccgga
     5521 tgccatcatc accgagtcgg gcttgaagat cacgccgctg gatgcggcgg tgcccgacac
     5581 cgcgcaggcg ctgatagacc agacagccat ggtcctgccg cacgtcaaga tcaccgaact
     5641 gctgctcgaa gtcgatgagt ggacgggctt cacccggcac ttcacgcact tgaaatcggg
     5701 cgatctggcc aaggacaaga acctgttgtt gaccacgatc ctggccgacg cgatcaacct
     5761 gggcctgacc aagatggccg agtcctgccc cggcacgacc tacgcgaagc tcgcttggct
     5821 gcaagcctgg catacccgcg acgaaacgta ctcgacagcg ttggctgaac tggtcaacgc
     5881 tcagtttcgg catccctttg ccgggcactg gggcgatggc accacatcat catcggacgg
     5941 acagaatttc cgaaccgcta gcaaggcaaa gagcacgggg cacatcaacc caaaatatgg
     6001 cagcagccca ggacggactt tctacaccca catctccgac caatacgcgc cattccacac
     6061 caaggtggtc aatgtcggcc tgcgcgactc aacctacgtg ctcgacggcc tgctgtacca
     6121 cgaatccgac ctgcggatcg aggagcacta caccgacacg gcgggcttca ccgatcacgt
     6181 cttcgccctg atgcacctct tgggcttccg cttcgcgccg cgcatccgcg acctgggcga
     6241 caccaagctc tacatcccga agggcgatgc cgcctatgac gcgctcaagc cgatgatcgg
     6301 cggcacgctc aacatcaagc acgtccgcgc ccattgggac gaaatcctgc ggctggccac
     6361 ctcgatcaag cagggcacgg tgacggcctc gctgatgctc aggaaactcg gcagctaccc
     6421 gcgccagaac ggcttggccg tcgcgctgcg cgagttgggc cgcatcgagc gcacgctgtt
     6481 catcctcgac tggctgcaaa gcgtcgagct acgccgccgc gtgcatgccg ggctgaacaa
     6541 gggcgaggcg cgcaatgcgc tggcccgtgc cgtgttcttc aaccgccttg gtgaaatccg
     6601 tgaccgcagt ttcgagcagc agcgctaccg ggccagcggc ctcaacctgg tgacggcggc
     6661 catcgtgctg tggaacacgg tctacctgga gcgtgcggcg catgcgttgc gcggcaatgg
     6721 tcatgccgtc gatgactcgc tattgcagta cctgtcgcca ctcggctggg agcacatcaa
     6781 cctgaccggt gattacctat ggcgcagcag cgccaagatc ggcgcgggga agttcaggcc
     6841 gctacggcct ctgcaaccgg cttagcgtgc tttattttcc gttttctgag acgaccccta
     6901 ttatggcgaa ggtatcattc aggaagcgtt gatgaaggat tatctctgta aaaagttatt
     6961 caaccggctt tccggtaccc tggtgatcag agcgcgctgc ggcaataaca ttactggcct
     7021 ggcatgttgc aatattcttt atccctcgcc ccgatacagc ggtcagctgc atattaaaga
     7081 gctgtatgtt tctcagtgcg atcgaaataa gggcacgggt aaagcgataa tgcgctttat
     7141 agcccggctt gcgcttgaac aggaatgcct tagccttagc tggaacgctg aaaaatccaa
     7201 ccccggcgct aaccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa
     7261 ttattacctc cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg
     7321 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
     7381 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
     7441 ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc
     7501 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     7561 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     7621 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     7681 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     7741 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     7801 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     7861 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     7921 caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc
     7981 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     8041 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     8101 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     8161 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     8221 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     8281 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
     8341 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt c
//
LOCUS       DAJTXY010000150         8351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTXY010000150 DAJTXY010000000
VERSION     DAJTXY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.150"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..158
                     /locus_tag="OO566_003637"
     CDS             <1..158
                     /locus_tag="OO566_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606752.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO566_003638"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO566_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6606753.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO566_003639"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO566_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6606754.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1293
                     /locus_tag="OO566_003640"
     CDS             874..1293
                     /locus_tag="OO566_003640"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6606755.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1280..6145
                     /gene="traI"
                     /locus_tag="OO566_003641"
     CDS             1280..6145
                     /gene="traI"
                     /locus_tag="OO566_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR6606756.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISSEQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSLVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQVETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPR
                     LEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEA
                     REQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWE
                     NNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRV
                     GTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQAYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPT
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQA
                     VREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVR
                     DLQKEKTPGGD"
     gene            6165..6911
                     /gene="traX"
                     /locus_tag="OO566_003642"
     CDS             6165..6911
                     /gene="traX"
                     /locus_tag="OO566_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6606757.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6966..7526
                     /locus_tag="OO566_003643"
     CDS             6966..7526
                     /locus_tag="OO566_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6606758.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPWLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7657..7869
                     /locus_tag="OO566_003644"
     CDS             7657..7869
                     /locus_tag="OO566_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6606759.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8113..>8351
                     /locus_tag="OO566_003645"
     CDS             8113..>8351
                     /locus_tag="OO566_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6606760.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1917 a         2094 c         2673 g         1667 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcctgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca tcacccgtgt ggaggcactg gcctccacac gggtgatgac
     1261 ggacggacag tcagaaacgg tgctgaccgg taatctggtg atggcactgt ttaaccacga
     1321 caccagtcgc gatcaggaac cacagttaca cacgcatgcg gtggtgacta atgttacgca
     1381 gtataacggc gaatggaaga cactgagcag tgacaaagtg gggaaaacgg ggttcattga
     1441 gaatgtgtac gctaatcaga ttgcctttgg caggctctac cgggaaaaac tgaaagagca
     1501 ggttgaggcg ctgggctatg aaactgaagt ggtcggtaag cacggtatgt gggaaatgcc
     1561 gggtgtaccg gtggaggcct tttccggacg ctcacagact atccgggagg ccgtcgggga
     1621 agacgcctcg ctgaaatccc gggatgtggc ggcgctggat acgcgtaaat ccaaacagca
     1681 cgtcgatcct gaagtcagaa tggccgagtg gatgcagacg ctgaaggaaa ccgggttcga
     1741 catccgggca tatcgtgacg cagcggaaca acgggcatat acccgcacgc agacacccgg
     1801 acccgcttca caggacgggc cggatgtgca gcaggcggtg acacaggcca ttgccggatt
     1861 aagtgaacgc aaagtgcagt ttatgtatac ggacctgctg gccaggacgg tcggcatact
     1921 gccgccggaa aatggtgtga ttgaacgggc acgcgccggt atcgatgagg ccatcagcag
     1981 tgagcagctt atccccctcg atcgtgagaa ggggctgttc acgtccggga ttcatatgct
     2041 cgatgagctg tcagtccggg cactcagtcg tgacatcatg aaacagaacc gggtgaccgt
     2101 acatccggag aaaagtgtcc cccggacggc cggttacagc gatgccgtca gtgttctggc
     2161 acaggaccgt ccgtcgctgg ccattgtgtc cgggcagggc ggtgcagccg ggcagcgtga
     2221 gcgggtggct gaactggtca tgatggcccg ggagcagggg cgggaggtgc agattatcgc
     2281 tgctgaccgt cgctcgcaga tgaacctgaa gcaggatgaa cggctgtccg gtgaactgat
     2341 aaccggacgt cgtcagttgc tggaaggcat ggccttcacg ccgggcagta ccgttatcgt
     2401 cgaccagggc gaaaaactct ccctgaaaga gacgttaacc ctgctggatg gtgccgcacg
     2461 tcataacgta caggtcctga taaccgacag cgggcagcga accggtacag gcagtgcact
     2521 gatggccatg aaggatgccg gggtgaacac atatcgctgg caggggggag aacagcgacc
     2581 ggccaccatc atcagtgaac cggaccgtaa tgtccgctat gcccggctgg ccggagattt
     2641 tgcggccagc gtgaaagccg gagaagagag cgtggcacag gtcagcggag tacgggaaca
     2701 ggccatactg acacaggcca ttcgcagtga gctgaaaaca cagggcgtgc tcggacaccc
     2761 ggaggtaacc atgaccgccc tgtcactggt ctggctggac agccggagcc gttatctgcg
     2821 ggatatgtac cgcccgggaa tggtgatgga gcagtggaac ccggagacac gcagtcatga
     2881 ccgctatgtg acagaacgtg tgacggcgca gagtcacagc ctgaccctgc ggaatgcgca
     2941 ggtcgaaacg caagtggtgc gtatttcttc cctggacagc agctggtcgc tgttccggcc
     3001 ggaaaaaatg ccggtggcag acggtgagcg tctgagggtg acagggaaaa ttcccggact
     3061 ccgcgtctcc ggcggtgacc gcctgcaggt ggcatccgtc agtgaagatg cgatgacggt
     3121 tgttgtgccg ggacgggctg aaccggccac cctgcctgtg agcgattcac cgttcacggc
     3181 actgaagctg gagaacggct gggtggaaac gcccgggcat tccgtcagcg acagtgcgac
     3241 ggtttttgcc tccgtcacac agatggcaat ggacaatgcc accctgaatg gtctggcccg
     3301 cagtggtcgt gatgtccggc tgtattcctc actggatgaa acccgtactg cggaaaaact
     3361 tgcccgccat ccctccttta cggtggtttc tgagcagata aaggcgcgtg ccggtgagac
     3421 attgctggaa accgctatca gtctgcagaa agccgggctt cacacgccgg cacagcaggc
     3481 cattcatctg gcccttcctg tgctggaaag taaaaacctg gccttcagca tggtggacct
     3541 gctgacagag gcgaagtcgt ttgctgcaga aggaaccggt tttgctgacc tgggagggga
     3601 aatcaatgcg cagataaaac ggggtgattt actgtatgtg gatgtggcaa aaggctatgg
     3661 cacaggcctg ctggtttccc gtgcgtcgta tgaggcagaa aagagcattc ttcgccatat
     3721 tctcgaaggt aaggaggcgg tcacgccgct gatggagaga gtacctggcg aactcatgga
     3781 gaaactgaca tcaggacagc gcgccgccac ccgtatgata ctggaaacgt ctgaccgttt
     3841 cacggtggtg cagggctatg ccggtgtggg taagaccaca cagttccggg cggtgatgtc
     3901 agccgtgaac atgctgccgg agagtgaacg tccccgtgtc gtggggctgg ggcccacaca
     3961 ccgtgcggtc ggggagatgc gcagcgccgg cgtggatgca cagacactgg cgtcctttct
     4021 gcatgacacg cagcagcgca gcggagaaac gccggatttc agcaacacgc tgttccttct
     4081 cgatgagagc tctatggtgg gcaataccga catggcacgg gcatacgccc tgattgcggc
     4141 cggtggcggt cgggctgtgg ccagcggtga cacggaccag ctgcaggcca tcgcgcccgg
     4201 tcagcctttc cgtctccagc agacgcgcag tgctgccgat gtggtcatca tgaaggagat
     4261 tgtgcgtcag acgccggaac tgcgggaggc ggtatacagc ctgattaacc gggatgtgga
     4321 aagggcgctg tccgggcttg agcgtgtgaa accgtctcag gtgccacgtc tggagggcgc
     4381 atgggcaccg gagcactccg tgacggagtt cagtcacagc caggaagcga aactggcaga
     4441 agcgcagcag aaggcgatgc tgaaaggcga ggcttttccg gatgtcccca tgacactgta
     4501 tgaagccatt gtccgcgact ataccggcag gacaccggaa gcacgggagc agacgctgat
     4561 tgtcacgcac ctgaatgagg accggcgcgt actgaacagc atgattcatg atgcacggga
     4621 aaaggccgga gaactgggga aggtgcaggt catggtgcct gtcctgaaca cagcgaatat
     4681 acgcgacggg gagctgcgtc gtctctccac ctgggagaat aacccggatg cccttgccct
     4741 ggtggataat gtgtatcacc ggattgccgg tatcagcaag gatgacgggc tgataaccct
     4801 gcaggatgcg gaaggtaaca cgcggctgat ttcgccccgg gaggcggtgg ctgaaggcgt
     4861 cacgctgtac accccggaca ccatccgggt ggggaccggt gacaggatac gcttcacgaa
     4921 gagtgaccgg gagcgcggtt atgtggccaa cagcgtctgg acggtgacag cggtttccgg
     4981 tgacagtgtc acactttctg acggtcagca gacccgtgtg attcgccccg gtcaggagcg
     5041 ggcagagcaa catattgacc tggcctatgc catcaccgcc cacggtgcac agggggcgag
     5101 tgaaaccttt gccatcgcgc ttgaaggcac ggaaggtaac cggaaactga tggccggctt
     5161 tgagtcagcc tacgtggccc tgtcgcgtat gaagcagcat gtgcaggcgt acaccgataa
     5221 ccgtcagggc tggacggatg ccatcaacaa tgccgtacag aaaggaactg cccacgatgt
     5281 gtttgagccg aaaccggacc gggaggtcat gaatgcagag cggctgttca gtacggcgcg
     5341 ggagctgcgg gacgtggcgg caggccgtgc cgttctccgt caggcggggc tggccggggg
     5401 agacagtcct acacggttta ttgctccggg acgtaaatat ccgcaaccgt atgtggcact
     5461 gccggcgttt gaccgtaacg gcaagtccgc cggtatctgg ctgaacccgc tgaccacgga
     5521 tgacggaaac gggctgcggg gattcagcgg tgaaggtcgg gtaaaaggca gcggggatgc
     5581 gcagttcgtg gccctgcagg gcagccgtaa cggagaaagc ctgctggctg ataatatgca
     5641 ggatggcgtc cggattgccc gtgataatcc tgacagtggt gtggtggtaa gaatcgccgg
     5701 tgaaggccgt ccgtggaatc ccggtgccat caccggtggc cgcgtgtggg gggatatccc
     5761 ggacaacagc gtccagccgg gagccggaaa tggcgaaccg gtcacggcag aggtactggc
     5821 acagcggcag gctgaagagg ccatccgccg tgaaacggaa cgtcgcgcag atgaaattgt
     5881 ccgtaaaatg gcagagaaca aacctgacct gccggacggc aaaacagagc aggctgtcag
     5941 ggaaattgcc ggtcaggagc gtgaccgggc tgccataact gaacgggaag ccgcgctgcc
     6001 ggagagtgta ctgcgtgaac cacaacgggt gcgggaagcg gtccgggaag ttgcccggga
     6061 aaatttgctg caggagcgtc tgcagcagat ggagcgggat atggttcgtg acctgcagaa
     6121 agagaaaacc ccgggcggag actgatacag gaagacaaac tctgatgaca acggataaca
     6181 cgaacacgac acgtaacgat tcactggctg cacggaccga tacctggttg cagtcatttc
     6241 tggtctggtc acccggacag cgggatatca tcaaaacggt ggcactggtg ctgatggtac
     6301 tggaccacat caatctgata ttccagctga agcaggaatg gatgtttctg gccgggcgag
     6361 gagcctttcc gctttttgcc ctggtgtggg ggctgaatct gtcccgtcat gcgcatatcc
     6421 ggcaaccggc cattaaccgg ctgtggggat ggggaattat tgcccagttc gcatattatc
     6481 tggcaggctt tccttggtat gaggggaata tcctgtttgc ctttgcagtg gcggcacagg
     6541 tgctgacgtg gtgtgaaacg cgcagtgggt ggcgtacagc agcggccata ctcctgatgg
     6601 ccctgtgggg gcctttgtcc ggcaccagtt acggtattgc cgggttgctg atgctggcag
     6661 tcagcaaccg gctgtaccgg gcggaagaca gagcggaacg tctggcgctg gtcgcctgcc
     6721 tgctggctgt tattccggcg cttaaccttg ccaccagtga tgcagcggcg gtggccggtc
     6781 ttgtgatgac ggtgctgacc gttggtctgg tgttgtgtgc cggaaaatca ttaccccgtt
     6841 tctggcccgg agactttttc ccgacgttct atgcctgtca tctggctgtg ctgggcgttc
     6901 tggcgctgtg acgggtgtgg tatctttggc cgcaagagga tgattcgtca gaggcagaac
     6961 acagcatgat agagcagaag cgaccggtac tgacactgaa gcggaagaca gagggaacag
     7021 cgcctgtccg cagccggaaa accatcatca atgtcaccac gccaccaaag tggaaggtga
     7081 aaaagcagaa gctggccgag aaagcggccc gtgaagcaga gctggcggca aaaaaagcgc
     7141 aggccagaca ggcgctgtcc atttatctga acctgcccac gctggatgag gctgtgaata
     7201 ccctgaagcc ctggtggccg ggattatttg acggtgacac gccctggctt ctggcctgcg
     7261 gtatccggga cgtgttactg gaagacgtgg cgcagcggaa tatcccgctc tcgcataaaa
     7321 aactgcgcag ggcgctgaag gccatcaccc gttcagaaag ctatctgtgt gccatgaaag
     7381 ccggtgcctg ccggtatgac acggaagggt atgtgacgga gcatatttct caggaggagg
     7441 aagcgtatgc gggagcgcgt ctggcgaaaa ttcgtcgcca gaaccggata aaggcagaac
     7501 ttcaggccgt gcttgatgag aaataaaaaa agcctccccc agagagagga ggcagggaaa
     7561 taaggcttaa aaggaaataa cttccgcttc cgaaaggaag ccagtccact gaattattta
     7621 tcacaataat actgacaact aaagggggac aagaaaatga atggattcag aaacagttca
     7681 cggaacggcc aggtctggcg ttaccagcgt gccggcgggc gggccgttat tctggaagtc
     7741 agtggacgct ggatggaagc ggcagaagca tggcgacggg ctgcctgtgt tgcgcctagg
     7801 acagactggc agcagtttgc ccggaaaagg gctgaacact gccaccggcg ctgccgggga
     7861 agggtgtaac gaaaaaagca gccttcatta ctctaggagt cagaaggctg aaaggagttt
     7921 atgagatttg cttcttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca
     7981 gggtcttttc cgcattaagt cgatttttta tattttgtga ttaatttcac aaaataaggt
     8041 gttattcaat gtgtgctgca ctattcaggg gggctgaaat acaggtggca tttatctgat
     8101 atggagaata acatgaggaa atatattcca ctggtattat ttatcttttc atggccggta
     8161 ttgtctgcag atattcatgg acgggttgtt cgtgtactgg acggtgatac catcgaggtc
     8221 atggactcac tgaaggcggt gcggatcagg ctggtaaata ttgatgcacc ggaaaagaaa
     8281 caggattatg gccggtggtc aacagacatg atgaaatcac tggtcgcagg aaaaacggtt
     8341 accgtcacat a
//
LOCUS       DAJTXY010000151         8318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTXY010000151 DAJTXY010000000
VERSION     DAJTXY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.151"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            332..3181
                     /locus_tag="OO566_003646"
     CDS             332..3181
                     /locus_tag="OO566_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6606761.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3281..5200)
                     /gene="dhaR"
                     /locus_tag="OO566_003647"
     CDS             complement(3281..5200)
                     /gene="dhaR"
                     /locus_tag="OO566_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6606762.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5428..6498
                     /gene="dhaK"
                     /locus_tag="OO566_003648"
     CDS             5428..6498
                     /gene="dhaK"
                     /locus_tag="OO566_003648"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6606763.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDVPVHTPALNWGK"
     gene            6509..7141
                     /gene="dhaL"
                     /locus_tag="OO566_003649"
     CDS             6509..7141
                     /gene="dhaL"
                     /locus_tag="OO566_003649"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6606764.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7152..>8318
                     /gene="dhaM"
                     /locus_tag="OO566_003650"
     CDS             7152..>8318
                     /gene="dhaM"
                     /locus_tag="OO566_003650"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6606765.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2173 a         2066 c         2076 g         2003 t
ORIGIN      
        1 cataactaat taggattatt acaaaaaatc ttcaccaaat tttttaaccg ctgtttgttg
       61 taatattttt gccatattca tagtaacgaa ttggaataag taacgacaac ttagggaata
      121 actcgatata aactcacctt tgtaaggggg gtgttaaata tatattaatt cctgtgttcc
      181 tgattttgcc cggtaataga gaattattct cagagaagct catcatgatt gcaccataca
      241 gatatcatga tgataagagg atattattca ttgacttaaa caaaatgaca aaatccatag
      301 acccatagcc tgggtactat ataggagtgg tatgggcatc aaacaacaca atgggaatac
      361 caaagccgat cgtctcgctg aattaaaaat ccgttcgccc tcaattcaac tgataaaatt
      421 tggcgctatt ggtttgaatg caattatctt ttcccccctg ctgatagctg ctgatacagg
      481 aagtcaatat ggcaccaata ttactattaa tgatggtgac agaattacag gagataccgc
      541 cgatccatca ggaaacctct atggtgtaat gaccccagca ggaaacacgc ctggcaatat
      601 caacctgggt aatgatgtca ccgtcaatgt caacgacgcc tctggatatg caaaaggaat
      661 cattattcag ggcaaaaaca gctccctgac agctaaccga ctcacagtag atgttgttgg
      721 tcaaacctct gccatcggca ttaacttaat tggtgactat acccatgctg acttaggcac
      781 aggcagcacc attaagagta acgatgacgg catcattatt gggcatagct caacactaac
      841 agccactcaa ttcaccattg aaaactcgaa cggtataggc ctaaccatca atgactatgg
      901 caccagtgtc gatcttggaa gcggaagtaa aatcaagacc gatggaagta caggtgttta
      961 tatcggtggt ctcaacggca ataacgccaa tggtgctgcg cgttttacgg cgacagacct
     1021 gacaatcgat gttcagggct acagcgccat ggggataaac gtacagaaaa actctgttgt
     1081 cgatctcgga acaaacagta ccattaaaac caatggcgat aatgctcacg gcctctggag
     1141 ctttggccag gttagcgcga atgcactcac tgttgatgta actggagccg cggccaatgg
     1201 cgtcgaagtt cgtggtggta caaccactat cggtgcagat agccatattt cttccgcgca
     1261 gggcggtggc ctcgtcacca gtggttcaga cgcgacaatc aatttttctg gcacggcagc
     1321 gcaacgaaac agcatctttt ccggcggttc ttatggtgcc tcggcccaga cggcaacggc
     1381 tgttatcaac atgcaaaata ccgatattac ggttgatcgt aatggcagtc tggcgctggg
     1441 tttgtgggcg ctcagcggcg gtagaataac cggagacagt ttggctatca ccggcgcggc
     1501 aggagccaga gggatttatg ccatgaccaa cagccagatc gacctcacga gcgatctggt
     1561 cattgatatg agtacacccg accagatggc catcgcaacg caacatgacg atggttatgc
     1621 caccagccgc atcaacgcct cgggtcgtat gcttatcaac ggtagcgttc tttccaaagg
     1681 tgggctaatc aatctggata tgcaccctgg gtcggtttgg acaggttcct ccctcagcga
     1741 taatgtcaat ggcgggaaac tggacgttgc aatgaataac agcgtctgga acgtaacaag
     1801 taattctaat ctcgacacgc tggcgctgag ccattcaact gtcgattttg ccagccacgg
     1861 gtcaactgcc ggcacattta ccacattaaa cgtagagaac ctgagcggta acagtacctt
     1921 tattatgcgt gctgatgttg ttggcgaggg taatggcgtt aataatagag gggatttatt
     1981 gaatatcagc gggagtagtg ctggtaatca cgtattggct atccgcaacc agggcagcga
     2041 ggccacaacg ggaaatgaag ttctgacagt ggtaaaaacc actgacggcg cggcctcgtt
     2101 cagcgcgtct tctcaggttg agttgggggg atatctgtac gatgtgcgta aaaatggcac
     2161 taactgggag ctttacgctt ccgggacagt tccggaaccg actcctaatc ctgaacccac
     2221 accagctccc gctcagcctc ccatagtcaa ccccgatcct acgcctaaac ccaccacgac
     2281 cgcagatgct ggcggcaatt atctcaatgt cggttactta ttgaactatg ttgaaaaccg
     2341 tacgctgatg caacggatgg gtgacttgcg aaatcagagt aaagacggta atatctggtt
     2401 gcgcagttat gggggaagcc tggactcctt tgccagtggc aaactgagcg gctttgacat
     2461 gggttacagc ggtatccagt ttggtgggga taaacgtctc tctgatgtaa tgccgttgta
     2521 tgtcggtctg tatattgact caacacatgc atcgccggac tatagcggag gcgacggtac
     2581 cgcacgttca gactacatgg gaatgtacgc cagttacatg gcacaaaacg gtttttacag
     2641 cgatctcgtt ataaaagcat cgcgccagaa aaatagtttc cacgtactgg acagtcagaa
     2701 caactgcgtt aacgccaacg gcactgcgaa tggaatgagc atctccctgg aagccgggca
     2761 gaggttcaac ctgtccccta ctggttatgg gttctatata gagccgcaaa cccagcttac
     2821 atacagccac cagaatgaga tggctatgaa ggcgagtaat ggcctcaata tacatctgaa
     2881 tcactacgaa tcgctgctgg ggcgtgccag catgatactg gggtatgaca tcaccgcagg
     2941 caacagccag ctgaatgtct atgtgaagac tggcgctatc cgcgagtttt caggggatac
     3001 cgaatatctg ttgaacgact cccgggagaa gtacagtttc aaaggtaatg gctggaataa
     3061 cggcgtggga gtcagtgcac agtataacaa acagcacaca ttctatctcg aagcggatta
     3121 cacgcagggt aacctctttg atcagaagca agtcaacgga ggatatcgct tcagctttta
     3181 agtgcgggat agacaagccc ttaaacgatg ctgaaaattg ttagcgtgcg ccggaatgaa
     3241 atacagatca ctctgatgta tcagaaatcg tttctgtctt tcatacccgg cgcttaaact
     3301 gccctgcatc aatgccatgt tgcttcattt tccgccacag cgtagtgcgg ccgatcccaa
     3361 gtaaagccga catttcctga atgcgaccgc ctgtgacctg ggctgcgtta ataattgcct
     3421 ctttttcaac ttccgcaaat gacagactgg tggaaaggcg ggtggcgctg acatcatctg
     3481 tcgcctgctc ggtaaacaga tgttcaggta aatcactgac gcgaatgcgc ccgttatcac
     3541 tactcagagc aagattctcg atgacgctgt aaagttcaaa atcgttgcct ggccatgcac
     3601 aagaaactag gcgagcgagg gcatcgtcat caattttcag ccgcgtagag aagcgttttt
     3661 caagactgcg taatttgtta ttcaccagcg ccggaatgct gccacgccgc atacgcagag
     3721 gcgggatggt aatttcaaat gcatgcagcg cgtaatacag ctggcgacta aaacgatttt
     3781 gttccaccag cattgcgagg tccgcggtcg ttgttgcaat cactttgaca tcaattggta
     3841 ttaaacgccg cgcatccagt cgcgtgataa ccccctgctt gataacctgc agcaaagcag
     3901 actgtaactc caccgccaga tattcaatct tttcaagaaa cagcgtgccg ccgtgtgcca
     3961 gttccagccg actcagacgg ccattttcat tgtccgtgcg atcgccacca ataaattctt
     4021 ccgccagcgc agcatcacca tataactcac aattgacggc gatataagga cctgcagcac
     4081 gctcgctttc attatgaatt gcctgactta gcagtgcctt gcccacgccc tcttctccac
     4141 aaagcaggac aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc aagcggcggg
     4201 tttgcggatc gtcctgtggc atatgagcga aggtatggct gacttttcct aattgactgg
     4261 tcattaactg ccgcatctgt tccacaggat ggagcaacaa aataaagctg gttccctgcg
     4321 tttctattat cggtttaagg gttatcaccg catcaataaa ctggtgctgg ctttcaaagg
     4381 ttgcttctac gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat acggcgggta
     4441 acgttaagag ttcagtgatt gcccgtccct gacttgccgt cgcgtcaagg cgcaagaccc
     4501 gcgccgcctg ggcattaata aattgcaaat taccctgctc gtcccagcta atcacgccat
     4561 catccatact ttctaacagg gcattaagtt gatttaaatg acggttagtt tcagcgagca
     4621 aactgtccgt cagcagtaaa ttaccgacct cgcgggcgat tgccaacgtc aacggcaaat
     4681 cagctgcggt agtttgctca accggacacg ccagcgctat tgttcccgtc aatcggccct
     4741 tgctgtcaaa caacggcgtt gcacaaaagg cccagttcca gagtgcctgt ttgaaatgtt
     4801 gatcggccat cgttttcacg gcctgaccag agatagccgc taacgatagc gcacaagtac
     4861 caataattcc ctcggcgcaa tacgtgccgt cattgaatcc cagtgcactt agctgctgca
     4921 aggtttgcgg atcgccatta cggctgagaa tgcaggcggt ttcatcgagg ataaacagcg
     4981 cacactctcg cggtgccata tattcccagg catcttccag cgcagcctgc ccgagcgtca
     5041 gcatcgcttt cttacgccga taaatagaag caaatgtcac gccctgggcc tgatgtggta
     5101 cgttccatgt ctcccgtttc atcagcttat tgcatcgctc ccaggaggtt gcaattaagg
     5161 gagatatgcc ccgaccatcg ttgttaaaag cgccactcat atcccgcatc ccttatgaca
     5221 aaaggtgttc cggctcgcaa ttttaagtat taatacacgt aaaagtttca atatggaaca
     5281 tttattaaag catatgtttc actatgaaac ataatttggg ctaattttct tttcccttgc
     5341 ctgatgcaca atggattcat cggcagtcag tggtcgccgt gtcgttgaac atcatccatg
     5401 ccctaccgta attgctggcg caaaataatg aaaaaattga tcaatgatgt gcaagacgta
     5461 ctggacgaac aactggcagg actggcgaaa gcgcatccat cgctgacact gcatcaggat
     5521 ccggtgtatg tcacccgagc tgatgcccct gttgcaggaa aagtcgccct gctgtcgggt
     5581 ggcggcagcg gacacgagcc gatgcactgt ggttatatcg gtcaggggat gctttcgggg
     5641 gcctgtccgg gcgaaatttt cacctcaccg acgcccgata aaatctttga atgctccatg
     5701 caaattgatg gcggcgaagg tgtactgttg attatcaaaa attacaccgg cgatattctt
     5761 aactttgaaa cagcgaccga gttactgcac gatagcggcg taaaagtgac cactgtggtc
     5821 attgatgacg acgttgcggt aaaagacagt ctttataccg ccgggcgacg cggcgttgcc
     5881 aacaccgtat taattgaaaa actcgtaggc gcagcggcgg agcgtggcga ctcactggac
     5941 gcctgtgcgg aactggggcg taagttgaat aatcaaggcc actcaatagg tatcgctctc
     6001 ggtgcctgta ccgttcctgc cgcgggcaaa ccttctttta cccttgcgga taatgagatg
     6061 gagtttggcg tcggcattca tggtgagcca ggtattgacc gccgcccttt ctcttccctt
     6121 gatcaaaccg tcgatgaaat gttcgacacc ctgctggaac atggctcata ccatcgcact
     6181 ttgcgtttct gggattatca acaaggcagc tggcaggaag aaccacaaac caaacaaccg
     6241 ctccagtctg gcgatcgggt gattgcactg gttaacaatc ttggcgcaac tccgctttct
     6301 gagctgtacg gcgtctataa ccgcctgacc acacgttgcc agcaagcggg attgactatc
     6361 gaacgtaatt taattggcgc gtactgcacc tcactggata tgaccggttt ctcaatcacc
     6421 ttactgaaag ttgatgacga aacgctggct ctctgggacg tcccggtcca caccccggcc
     6481 cttaactggg gtaaataagg agaaagcaat gtcactgagc agaactcaaa ttgttaactg
     6541 gctcactcgt tgtggcgata ttttcaacac cgagagcgag tatcttaccg ggctggatcg
     6601 cgagattggc gacgctgacc acgggctaaa tatgaaccga ggctttagca aagtggtgga
     6661 aaaactccct gctatcgcag ataaagatat cggtttcatt ctcaaaaata ccggtatgac
     6721 gctgctttcc agcgtcggtg gtgccagtgg tccgctgttc ggtaccttct ttatccgcgc
     6781 cgcacaggcg acccaggcac ggcaaagcct gacactggaa gagctttatc agatgttccg
     6841 cgatggcgcg gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata aaaccatgtg
     6901 tgatgtgtgg gtgccggtgg tggaatcgtt acgtcagtcc agcgagcaaa atctctctgt
     6961 tccggtggcg ctcgaagctg ccagtagcat cgccgaatcc gcggcacaaa gtacgattac
     7021 gatgcaagcc cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg gtcaccagga
     7081 tcccggcgcg acctcggtga tgtttatgat gcaaatgttg gcgttagccg caaaagagta
     7141 aggaattggt gatggtaaac ctggtcatag tttcacatag cagccgactg ggagaaggtg
     7201 tcggtgaatt agcccgtcag atgttaatga gtgatagttg taaaatcgcc attgccgcgg
     7261 gaattgacga tccacaaaat cccattggta ccgatgccgt caaagtgatg gaggccatcg
     7321 aatctgttgc tgatgccgac catgtgctgg tcatgatgga tatgggtagc gcattattga
     7381 gtgctgaaac tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta cgtttgtgtg
     7441 ctgcgccgtt ggtcgaaggt acactggcag caacggtcag cgcggcctcg ggggcggata
     7501 tcgacaaagt tatctttgac gctatgcatg cgctggaagc caaacgtgaa caactgggtt
     7561 taccgtcctc cgacactgaa atctctgaca catgtcctcc gtacgatgaa gaagcccgtt
     7621 ctctggcggt ggtcataaaa aaccgtaacg gcctgcatgt acgtccggcc tcccggctgg
     7681 tttatacctt atcgacattt aatgccgata tgttgctgga aaaaaacggc aaatgcgtca
     7741 caccagagag tattaaccag attgcgttac tacaagttcg ctataacgat acgctgcgcc
     7801 tgattgcgaa agggccagaa gctgaagagg cactgatcgc tttccgtcag ctggctgaag
     7861 ataactttgg tgaaacggag gaagtcgctc cacctactct gcgtcccgtt ctgcctgttt
     7921 cgggtaaagc cttttattat caaccagttt tatgtacggt acaggcaaaa tcaaccctga
     7981 ccgcggaaga agaacaagat cgattacgcc aggctattga cttcacgtta ttagatctga
     8041 tgacgttaac agcgaaagca gaagccagcg ggcttgacga tattgccgca atcttttctg
     8101 gtcaccatac actgttaggt gatccggaac tgctggcggc ggcaagcgaa ctccttcagc
     8161 atgaacattg cacggcagaa tatgcctggc agcaagttct taaagaactt agccagcaat
     8221 accagcaact ggatgatgaa tatctacaag ctcgctatat tgatgtggac gatcttctgc
     8281 atcgcaccct ggtccacctg acccaaacga aagaagaa
//
LOCUS       DAJTXY010000152         8181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTXY010000152 DAJTXY010000000
VERSION     DAJTXY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.152"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..451
                     /locus_tag="OO566_003651"
     CDS             <1..451
                     /locus_tag="OO566_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6606766.1"
                     /translation="NNASYQLNNALPNKETISSNFCERLEQWGNKSVNNGEERAIAVE
                     RIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSV
                     NKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            561..1892
                     /locus_tag="OO566_003652"
     CDS             561..1892
                     /locus_tag="OO566_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6606767.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYIPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2216..3604
                     /gene="narU"
                     /locus_tag="OO566_003653"
                     /pseudo
     CDS             2216..3604
                     /gene="narU"
                     /locus_tag="OO566_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            3686..7319
                     /gene="narZ"
                     /locus_tag="OO566_003654"
                     /pseudo
     CDS             3686..7319
                     /gene="narZ"
                     /locus_tag="OO566_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            7316..>8181
                     /gene="narH"
                     /locus_tag="OO566_003655"
     CDS             7316..>8181
                     /gene="narH"
                     /locus_tag="OO566_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6606768.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTER"
BASE COUNT         2186 a         2026 c         1907 g         2062 t
ORIGIN      
        1 aaataatgcc agttatcaac ttaataacgc attacctaac aaagaaacca tctccagcaa
       61 tttttgtgaa cgactagaac aatggggtaa taaatcggtt aataatggtg aagaaagagc
      121 aattgccgtg gagcgaatta aagaagctta caattcgaac atggcatctc ttgatttgtc
      181 atatcttgat ttgagtgaat taccgcctat cccctcgaca gttaatacgt taaatctgga
      241 aaacaattgt ctaacttgtc ttgacttagc tgacattgcc agtctcgtta atatcaacct
      301 cagcgttaat aaaattaaca cgataacctt tccaaaagaa ccaaaactgg aaaacattta
      361 tattgatcat aacaatctgg aaagtttaga tttaaaaaat cagcattcat tggttaatct
      421 ggaagcgcaa aataacagcc taaaaaaatg atatttctga tagttataaa ctgaaatttc
      481 ttaatcttga ttataacaag ctagcatcgc tggatgtctc ccggcaagaa tctctgattg
      541 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaagatga
      601 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
      661 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
      721 tatcatcaaa acatctttgg catgtattag ttaacggcat caacaatgat ccacttgcac
      781 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
      841 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acctctatca
      901 atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga ctcgtaaagc
      961 taagtctgaa tagtaatgct cttgagtcaa taaattttcc tcaaggcaga aatgtaagta
     1021 ttacacatat atctatgaat aataatgctc tcagaaatat tgatatagat aggctttcat
     1081 cagttactta ttttagtgcg gcacataatc aactagagtt tgtgcaatta gaatcttgcg
     1141 aatggctgca atacctgaat ctcagccata atcaattaac tgatattgtt gcaggtaata
     1201 aaaatgaact cttactgctg gatctatccc ataataaact aacaagttta cacaatgtct
     1261 tatttcccaa cttgaatacg ttacttatta acaacaattt gctttctgaa attaaaatat
     1321 tctatagcaa cttccgcaat gttcagacat taaacgctgc taacaaccag ttgaaatata
     1381 taaatcttga tttcctgact tatcttccat ctatcaaaag tttaagactg gacaataata
     1441 aaataaccca cattgatact aataatacat ccgatattgg aactttattc cccataataa
     1501 aacagagcaa aaacttaaat tttttaaatg tttctgggaa gaacaattgc cctactatgc
     1561 agctcatgtt atttaatttg ttttccccag cacttaagct taatactggc ctggcaattc
     1621 tttcgcctgg tgcatttgaa gttcactctg acggaataga tgtggataac gaattgtttc
     1681 actatactat taaaaaagca tatatcccat ataatataca cacttacaag acagaggaag
     1741 ttgtaaacca gaggaatata aaagttaaaa acatgacctt agatgaaata aacaatactt
     1801 actataataa cgattattac aatcaggcaa taagagagga accgatagac tttctggaca
     1861 gatcgttttc ctccaattca tggccttttt agtgaataaa tcatcactaa ttctataagt
     1921 gtctggtctt gttgagaata tagttaatat gtaacccttt agattcacga tctgaagggt
     1981 tgaaacatga catacgttca aagattaacc cccgtttgaa ggttagcttg ttagttacat
     2041 ttagtaacac atatctggct acttaatccc cattctgccc ccgcggttac gctttcttat
     2101 ctattctttc aatcagcact tcacccaatt catccttaat tctaataact ggctattgcg
     2161 ccttacctcc tgttttgtct tttttcatta ccaatgtgtg catgtgagga acaatatggc
     2221 actgcaaaat gagaaaaata gtcgttatct tttacgcgac tggaaaccag aaaatccggc
     2281 cttctgggaa aataaaggaa aacatattgc tcgaagaaac ctctggatat cagtcagttg
     2341 tctacttctt gccttctgtg tctagatgct atttagcgca gttaccgtta atctcaataa
     2401 aatcggtttt aattttacta ccgatcaact ctttttatta accgcattac cctccgtttc
     2461 tggcgcatta ttgcgtgttc cctactcctt tatggtgcct atattcggtg gacgccaatg
     2521 gccggttttt agtactgcaa tcctgattat tccttgcgtc tggctcggaa ttgccgtgca
     2581 aaatccgaat actccttttg ggatatttat cgttatcgct ttgctatgcg gttttgcagg
     2641 tgcaaacttt gcttcgagca tgggcaatat cagtttcttc tttccaaaag ccaaacaagg
     2701 gagcactctt gggattaatg gcggattagg aaacttaggt gtaagtgtaa tgcagctggt
     2761 tgcaccgctg gtcatttttg tacctgtatt tgcctttctc ggcgtcaatg gcgtaccgca
     2821 ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg atttgggtac cgctactggc
     2881 gattgccacg atcgccgcct ggtcagggat gaatgatatc gccagttcac gcgcctcaat
     2941 tgccgaccag ctccctgtct tacaacgcct gcatctctgg ctgctgagcc tgctttacct
     3001 tgccaccttc ggttcgttta tcggtttttc tgcgggtttt gccatgctgg caaaaaccca
     3061 gttcccggat gtgaatattc tgcgcctggc gttctttggc ccatttattg gtgccatcgc
     3121 gcggtcggtt ggtggtgcta tttccgataa attcggcggc gtgcgggtga cgttgatcaa
     3181 ctttattttt atggcgattt tcagtgccct gctgttcctt accttaccgg gcacaggctc
     3241 cggtaatttc atcgcctttt acgccgtatt tatggggctg tttctgaccg cgggtctggg
     3301 aagtggttct actttccaga tgatcgccgt catctttcgc cagataacca tttatcgggt
     3361 gaagatgaaa ggcggtagtg atgagcaagc tcagagagaa gccgtcaccg aaacggcggc
     3421 agcgctgggc tttatctcag ccattggcgc agtgggcggc ttttttattc cgcaggcatt
     3481 tggcatgtcg ctcaatatga ccggctctcc ggtcggcgcg atgaaagtgt ttttaatctt
     3541 ctacatcgtt tgtgtgctgc tgacctggct ggtttatggt cggcggaagt tcagccaaaa
     3601 ataagagatc gatgcctgat gtacaaccgt gtcgcatccg acaaggcatc agatcccaat
     3661 tcattcctgg agcaggagtc atgtcatgag taaacttttg gatcgctttc gctacttcaa
     3721 acaaaagggc gaaacctttg ccgatggtca cggacaggtg atgcatagca accgcgactg
     3781 ggaggacagc tatcgccagc gttggcagtt cgacaaaatc gtgcgttcca cccacggcgt
     3841 taactgtaca ggctcctgta gctggaaaat ctacgttaaa aatggtctgg tgacctggga
     3901 aatccaacag accgactacc cgcgcactcg ccctgacctg cccaatcatg aacctcgcgg
     3961 ctgcccgcgt ggcgcaagtt actcctggta tctttacagc gctaaccgcc tgaaataccc
     4021 gctcattcgt aaacgactga ttgaactgtg gcgcgaagcc ctcaaacaac acagcgatcc
     4081 ggtactggcg tgggcatcga ttatgaacga tccgcaaaag tgcctgagct acaaacaagt
     4141 gcgtgggcgc ggcgggttta tccgctccaa ctggcaggaa ctaaaccagc tgattgccgc
     4201 cgctaacgtc tggaccatca aaacctacgg cccggatcgc gttgccggtt tctcgccgat
     4261 cccggcgatg tcgatggttt cttacgccgc cggaacgcgt tatctgtcgc tgcttggcgg
     4321 cacctgttta agtttctacg actggtattg cgacctgccg cccgcctcgc cgatgacctg
     4381 gggcgagcaa accgacgtac cggaatctgc cgactggtat aactccagct acatcatcgc
     4441 ttgggggtct aacgtaccgc agacacgtac gccggacgcc cacttcttta ccgaagtacg
     4501 ctacaaaggc actaaaacca tcgccattac ccctgactac tctgaagtgg ccaaattgtg
     4561 cgaccagtgg ctggcaccga aacaaggcac tgatagcgcc ctggcgatgg caatggggca
     4621 tgtgatttta aaagagtttc atctcgataa tcccagcgac tactttatca actactgccg
     4681 ccgctacagc gacatgccga tgctggtaat gctggagcct cgcgacgatg gtagctacgt
     4741 tcccgggcgc atgatccgcg catctgacct ggtggatgga ctgggcgaaa gcaacaatcc
     4801 gcagtggaaa accgtagcag ttaataccgc aggtgaattg gtagtgccga acggttcgat
     4861 tggtttccgc tggggagaaa aaggcaaatg gaatctggaa tccattgccg ccggtacgga
     4921 aaccgaattg tcgttaaccc tgctcggtca acatgatgct gttgcaggcg tggccttccc
     4981 ctactttggc ggcattgaaa atccgcattt tcgcagcgta aaacacaatc cggtgctggt
     5041 gcgccaattg cccgttaaaa acctgacgtt agtcgatggc aacacctgtc cagtggtcag
     5101 cgtttatgat ttggtactgg cgaattacgg cctcgatcgc gggctggaag atgaaaacag
     5161 cgcgaaagat tacgctgaaa tcaaaccgta caccccagcc tggggtgagc aaattaccgg
     5221 cgtaccgcgc cagtatattg aaaccatcgc ccgcgaattt gccgataccg cccataaaac
     5281 gcatgggcgc tcaatgatta tcctcggcgc aggtgttaac cactggtatc acatggacat
     5341 gaactaccgc gggatgatca atatgctgat cttctgcggt tgtgtcgggc aaagcggtgg
     5401 cggctgggca cactatgtcg gccaggaaaa actgcgccca caaaccggct ggttgccact
     5461 ggcctttgcg ctcgactgga accgaccacc gcgccagatg aacagcacct cgtttttcta
     5521 caatcattcc agccaatggc gctatgaaaa agtctctgct caggagttac tttcaccgct
     5581 cgccgatgcc agtaagtaca gcggtcatct gattgatttc aacgttcgcg ccgaacgtat
     5641 gggctggcta ccttctgcgc cgcagctggg gcgtaacccg ctcgggatta aagctgaagc
     5701 cgacaaggcc ggattatccc ccacagaatt taccgcccag gcgctgaaat cgggcgattt
     5761 acgtatggcc tgcgaacaac cagatagcgg cagcaatcat ccgcgtaatt tgtttgtctg
     5821 gcgctctaac ctgcttggct cctccggcaa aggccacgag tatatgcaga agtatctgct
     5881 ggggaccgaa agcgggattc agggcgagga actcggtgcc agcgacggga tcaaaccgga
     5941 agaagtcgag tggcaaactg cggcgattga aggcaagctc gacctgctgg tgacgctcga
     6001 cttccgcatg tccagtacct gcctgttctc cgatatcgtc ctgcccaccg ccacctggta
     6061 tgaaaaagac gatatgaaca cctcggatat gcatccattt attcatccgc tttctgcggc
     6121 ggtcgatccg gcctgggaat cacgcagcga ctgggaaatc tacaaaggta ttgccaaagc
     6181 attttcgcaa gtgtgcgtgg gccatcttgg caaagaaacc gacgtggtat tacaaccact
     6241 gctgcatgac tctccggcag agctctcaca gccgtgtgaa gtgctcgact ggcgcaaagg
     6301 cgaatgcgat ctgatcccgg gtaaaaccgc gccgaatatt gtggcggtgg agcgcgacta
     6361 ccctgctacg tatgaacgct ttacctcgct cgggccattg atggacaaac ttggcaatgg
     6421 cggtaaaggg atttcgtgga atacccagga tgaaatcgat ttcctcggca aactcaatta
     6481 caccaagcgt gatggcccgg cacaggggcg tccgctgatt gacaccgcca ttgacgcttc
     6541 agaagtgatt ctggcactgg caccggaaac caacggtcat gttgcagtca aagcgtggca
     6601 ggcgctgggc gagatcaccg ggcgcgaaca tacccatctg gcgctgcaca aagaggacga
     6661 gaagattcgc tttcgcgata ttcaggcgca gccgcgtaaa attatctcca gccccacatg
     6721 gtccggtctg gaaagcgatc acgtatccta taacgcggga tacaccaacg ttcatgagtt
     6781 aattccgtgg cgcacgctgt cgggacgcca gcagctctat caggatcatc cgtggatgcg
     6841 tgcttttggt gaaagcctgg tggcttatcg cccgcctatc gacacccgta gcgtcagtga
     6901 gatgcgccag ataccgccaa acggcttcca ggaaaaagca cttaacttcc tgacgccgca
     6961 ccagaaatgg ggcattcact caacctacag tgaaaacctg ctaatgctga cgctctctcg
     7021 cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc caggaacgca
     7081 ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat aactcggtca
     7141 cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg gcctggggct
     7201 ttaactacta cggcaccgtc ggctcgaacc gcgacgaatt catcatgatc cgcaagatga
     7261 agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg aaaaaatgaa
     7321 aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct gccatacctg
     7381 ttcggtgacc tgtaaaaacg tctggaccgg acgcgaaggc atggagtacg catggtttaa
     7441 caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc aggaagagtg
     7501 gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg gcagcaagat
     7561 gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg atgattacta
     7621 cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca aacatatccc
     7681 tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga tctggggccc
     7741 aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc gcaacttcga
     7801 ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt acctgccgcg
     7861 cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg gcgctatcta
     7921 caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg gctggcgttt
     7981 gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg gcaagtcaga
     8041 aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt gctcagaaac
     8101 ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc gcatcgagga
     8161 agcggcgagc accgagcgcg a
//
LOCUS       DAJTXY010000153         8150 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTXY010000153 DAJTXY010000000
VERSION     DAJTXY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.153"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(51..590)
                     /locus_tag="OO566_003656"
     CDS             complement(51..590)
                     /locus_tag="OO566_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6606769.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(655..1203)
                     /gene="fimA"
                     /locus_tag="OO566_003657"
     CDS             complement(655..1203)
                     /gene="fimA"
                     /locus_tag="OO566_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6606770.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1684..2280)
                     /gene="fimE"
                     /locus_tag="OO566_003658"
     CDS             complement(1684..2280)
                     /gene="fimE"
                     /locus_tag="OO566_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6606771.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2758..3359)
                     /gene="fimB"
                     /locus_tag="OO566_003659"
                     /pseudo
     CDS             complement(2758..3359)
                     /gene="fimB"
                     /locus_tag="OO566_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            4813..5529
                     /gene="nanC"
                     /locus_tag="OO566_003660"
     CDS             4813..5529
                     /gene="nanC"
                     /locus_tag="OO566_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6606772.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5549..6655
                     /gene="nanM"
                     /locus_tag="OO566_003661"
     CDS             5549..6655
                     /gene="nanM"
                     /locus_tag="OO566_003661"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6606773.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNRWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKEEYYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6720..7700
                     /locus_tag="OO566_003662"
     CDS             6720..7700
                     /locus_tag="OO566_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6606774.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2420 a         1741 c         1697 g         2292 t
ORIGIN      
        1 tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa ttactgatag
       61 gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg cccggtagca
      121 cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt ccagtttgct
      181 ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac accaatattg
      241 gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc atccgcgaca
      301 ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg taaatgaata
      361 acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact gctgatttgc
      421 cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc cgcaataatg
      481 acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt tccggccaga
      541 gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat cgttattttt
      601 atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg taggttattg
      661 atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct cgccgattgc
      721 ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg tttgtgcact
      781 aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct gcacgccaac
      841 gtttgttgca ctacctgcag cagaactctg cagagctagt acatcagtac gcgtagcatc
      901 aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat cgcaatcatt
      961 cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca ggctagcggt
     1021 acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg cgcaagcggc
     1081 gttaacaact tcccctttaa agttaatggt cccaccattt accgtcgtag tatcggccag
     1141 agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag ttttaatttt
     1201 catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg ttctgtacac
     1261 tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa aagatgaaac
     1321 atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt cagtttattt
     1381 gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca tgaggtggtt
     1441 tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt tacatatagc
     1501 ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata gttagatgct
     1561 ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata cgtaacttat ttatgatatg
     1621 gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta tcaataagtt
     1681 aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt cccataatcc
     1741 ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat ttcgatgccc
     1801 gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat aaccgcaagc
     1861 atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac cggcatcgcg
     1921 aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa taaatatagc
     1981 gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt ccacggcttc
     2041 acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc ggcgaatatt
     2101 tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt cactaatacg
     2161 catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg ttgccccgta
     2221 acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac gtttactcac
     2281 aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa cctcaatcgg
     2341 tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg ctatcagtca
     2401 attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc tattttcatt
     2461 gatttgattc aatataacaa tagattacag aggattaact tccagttact taaaaaataa
     2521 agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa tgattttgtt
     2581 tatttatttt gaagaacata aaccattaga ttttgtatgg gtatggttaa cttaatgttt
     2641 attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt taatcatttt
     2701 tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca actatctcta
     2761 taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa aacgccctgc
     2821 attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat aatcctagat
     2881 aagtcgcgta tctattccta tattcgccaa agcaaaacca cacgaatagc gtaacatgtg
     2941 cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa tatggtaaaa
     3001 ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc tctcagcatg
     3061 cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt tgttcaatag
     3121 cgggtgcgtt gttgaaaagc ctttttttaa tcgatggata tatatacact ttgctttaag
     3181 atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat gaataaaaca
     3241 aagcaaaatc agacaataat tacgtgttgc atgaggccgg tatttgctgc tttaaggagt
     3301 gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc
     3361 gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
     3421 tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca
     3481 gttcttctga gcgaacttaa atggagtagc gatagttact tataataact ttgccataca
     3541 aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa
     3601 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
     3661 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttcaac tagccgtctc
     3721 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
     3781 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
     3841 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
     3901 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
     3961 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
     4021 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
     4081 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgagta
     4141 aacccttatt ttcaccactc tcactgagtt agcttgcaaa tctttctttg tttagattct
     4201 aaataaattt tttataaaac atatagttaa aagaaaatct ttagtttttg aagaccaccg
     4261 caagtgttcg tctggcttca tatggcatct tcttcttaga aaaagatcga catattttgt
     4321 gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac
     4381 aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
     4441 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
     4501 aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat
     4561 ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta
     4621 atctggatct ctgtttattt aaataatgtt aaaagagaat tttcacagga gaccttatac
     4681 aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat
     4741 tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa
     4801 tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg
     4861 tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc
     4921 cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc
     4981 atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat
     5041 gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg
     5101 ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc
     5161 tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac
     5221 cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat
     5281 gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa
     5341 cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc ctttgttcta
     5401 caataccata tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag
     5461 ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt
     5521 aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat
     5581 gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag
     5641 tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc
     5701 atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc
     5761 tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg
     5821 cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa
     5881 ccccaaaacc aatcgctggg ttaaattgat gtcgcacgcg ccgatgggca tggcgggtca
     5941 tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt
     6001 caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat
     6061 caacgcccac tattttgaca aaaaagaaga atattatttc ttcaataagt ttctgttgtc
     6121 ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc
     6181 tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc
     6241 aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa
     6301 taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa
     6361 tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa attaccagaa
     6421 cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg
     6481 gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc
     6541 attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt
     6601 gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt
     6661 cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc gaaaattata
     6721 tgaacgcaat aatatcgccc gattattact atgttcttac cgttgctggt cagtctaatg
     6781 ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct cattccagaa
     6841 ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgccatgt cactttaacg
     6901 acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt tatcaccatc
     6961 ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg catattgcac
     7021 ggaaattact gcccttcatt cctgataatg cagggattct tatcgttccg tgttgccgtg
     7081 gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac ggagccagcc
     7141 atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc agcagaacgc
     7201 gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg atgcaaggcg
     7261 aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat catatggttg
     7321 aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt actgacgcgc
     7381 cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat tcgtatgaag
     7441 ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc gacttccagc
     7501 aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg
     7561 gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg cgaagcagtc
     7621 atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa gcaattttgc
     7681 agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt gttcgttctc
     7741 agagtagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg cgggctcgaa
     7801 cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc accgcataaa
     7861 ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc cttttcgatt
     7921 atggctcgga aatctacgaa catctacaca gtttactgga acgttttggg ccacagcgtt
     7981 cgcagcaccg tcctgatatg ccgttctggc gattataagt cgacggattc tggcaaatag
     8041 acgatgatga tatgcttaat gatgttcaaa gaaattaggc cattattata ttattgcttg
     8101 cattttcttt cattcctaaa agcacccgcc attaatccca ggcgcattat
//
LOCUS       DAJTXY010000154         8145 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTXY010000154 DAJTXY010000000
VERSION     DAJTXY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.154"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..894)
                     /locus_tag="OO566_003663"
     CDS             complement(<1..894)
                     /locus_tag="OO566_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6606775.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            954..1232
                     /locus_tag="OO566_003664"
     CDS             954..1232
                     /locus_tag="OO566_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606776.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OO566_003665"
     CDS             complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OO566_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6606777.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OO566_003666"
     CDS             complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OO566_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6606778.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3386..3718
                     /gene="hdeA"
                     /locus_tag="OO566_003667"
     CDS             3386..3718
                     /gene="hdeA"
                     /locus_tag="OO566_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6606779.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3834..4160
                     /gene="hdeB"
                     /locus_tag="OO566_003668"
     CDS             3834..4160
                     /gene="hdeB"
                     /locus_tag="OO566_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6606780.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4224..4870
                     /locus_tag="OO566_003669"
                     /pseudo
     CDS             4224..4870
                     /locus_tag="OO566_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4912..5442)
                     /locus_tag="OO566_003670"
     CDS             complement(4912..5442)
                     /locus_tag="OO566_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6606781.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OO566_003671"
     CDS             complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OO566_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6606782.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6411..7634)
                     /locus_tag="OO566_003672"
     CDS             complement(6411..7634)
                     /locus_tag="OO566_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606783.1"
                     /translation="MSIDFTLGIINTYHGDIYNCTTNTDNVKTPDTPKWPYDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2366 a         1576 c         1704 g         2499 t
ORIGIN      
        1 gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt caccatttgg
       61 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg tcgggtcgga
      121 gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga gctgtactgg
      181 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
      241 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
      301 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
      361 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
      421 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
      481 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
      541 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
      601 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgctc tggttcactt tatcgccttc
      661 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg cagcttcata
      721 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaactagggg agagtgtgac
      781 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
      841 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
      901 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
      961 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     1021 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     1081 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat caagtcgttt
     1141 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     1201 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     1261 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     1321 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     1381 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     1441 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     1501 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     1561 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     1621 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     1681 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     1741 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta ttgtttcgaa catgtcatcc
     1801 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     1861 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     1921 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     1981 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     2041 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     2101 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     2161 agtaaataac gcatttatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     2221 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     2281 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     2341 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     2401 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     2461 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     2521 acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt aacgaacaaa
     2581 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     2641 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     2701 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     2761 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     2821 atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     2881 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     2941 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     3001 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     3061 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     3121 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     3181 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     3241 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     3301 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     3361 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     3421 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     3481 accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     3541 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     3601 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     3661 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     3721 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     3781 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     3841 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     3901 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
     3961 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
     4021 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
     4081 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
     4141 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
     4201 gctgctcctt tttgcatttt tatatgacag cagaattaat tatacgtctt atactcgcgg
     4261 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     4321 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
     4381 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
     4441 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
     4501 taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg ttattggcag
     4561 cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc tggaagtctt
     4621 ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga cattagtgaa
     4681 cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa ctgatttagt
     4741 ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc ttcatgccac
     4801 aacgttggta gaagacctgt tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc
     4861 aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa ttcacaccag
     4921 ataatcaata tgctgatggc gtaccagctc attgatccgt ttaagattga ggatcatcat
     4981 gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct ccagaatatc
     5041 ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc gcgaaagtga
     5101 cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa aatcaatgat
     5161 agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt taatattaaa
     5221 gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga gaaaattaga
     5281 atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat gcaacattac
     5341 gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa tgtttttcat
     5401 cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact cctggtgcgg
     5461 acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt ataataataa
     5521 tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg agaaagcatc
     5581 tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg
     5641 tagtaaggcg cacccaagcc cggttccggc cagaatgcac catagccgta atcccacagg
     5701 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
     5761 tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg aatggtaccg
     5821 aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg
     5881 agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat
     5941 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
     6001 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt
     6061 tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggcaagc
     6121 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
     6181 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggtgata
     6241 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag ttcaattccg
     6301 ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt gttttaaaat
     6361 tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata ttatggtatt
     6421 atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc tatttccacc
     6481 ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg gtatgaaatc
     6541 ttttcgggtt gaagtgtctc gtaatgaaat gctatgaatt catttttaga cattgttgcc
     6601 catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg tttataacat
     6661 atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt cactaccttt
     6721 gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa agagttgtca
     6781 ttaatagtta acaatgtaga aaacaagcca ttgatttgtt tgtcaagatg ttgttcatat
     6841 ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa taaatttgcg
     6901 tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt taggactagc
     6961 ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc aagggcttcc
     7021 atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa gtacaagctt
     7081 tgaatgtctc ttatatcggt tggtaagtca gctcgttgcc cagcaaaatt tttattatcg
     7141 gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag aattttagtt
     7201 atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac agaatcctta
     7261 tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg ttgcgtaagt
     7321 ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac cacaactttt
     7381 gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg ttggagttgt
     7441 tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt ggaattaatg
     7501 ggttgttgtt cttcccaatt atcataaggc cacttcggtg tgtctggcgt tttaacatta
     7561 tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat acctagggta
     7621 aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt ttttaaattg
     7681 gataattaga acaggaatgg attttcttct acacacaata aatataatgc gttatatctt
     7741 cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt gtgtataaaa
     7801 aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaag cgctcatttt
     7861 aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg ttttagatgt
     7921 gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga ctgaaatctg
     7981 ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga taaaatgtca
     8041 gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc cgtcatcatt
     8101 ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcct
//
LOCUS       DAJTXY010000155         8137 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTXY010000155 DAJTXY010000000
VERSION     DAJTXY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.155"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..671)
                     /gene="chbR"
                     /locus_tag="OO566_003673"
     CDS             complement(<1..671)
                     /gene="chbR"
                     /locus_tag="OO566_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR6606784.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIIN"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OO566_003674"
                     /pseudo
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OO566_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OO566_003675"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OO566_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OO566_003676"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OO566_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6606785.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OO566_003677"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OO566_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6606786.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="OO566_003678"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="OO566_003678"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6606787.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINLLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="OO566_003679"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="OO566_003679"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6606788.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OO566_003680"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OO566_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6606789.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREISTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OO566_003681"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OO566_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6606790.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..>8137)
                     /gene="astE"
                     /locus_tag="OO566_003682"
     CDS             complement(7177..>8137)
                     /gene="astE"
                     /locus_tag="OO566_003682"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6606791.1"
                     /translation="FLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQGALV
                     ISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDMNRM
                     FGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGVLPQ
                     RDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFGQND
                     LRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFMPFE
                     KGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2138 a         2034 c         1882 g         2083 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatacta tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttaca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tgcggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttaacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctggcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatctgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattcg acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtggaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca aaacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgctg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaa
//
LOCUS       DAJTXY010000156         8081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTXY010000156 DAJTXY010000000
VERSION     DAJTXY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.156"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..166
                     /locus_tag="OO566_003683"
     CDS             <1..166
                     /locus_tag="OO566_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR6606792.1"
                     /translation="THYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWW
                     WNMLIGWNML"
     gene            349..2529
                     /locus_tag="OO566_003684"
     CDS             349..2529
                     /locus_tag="OO566_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6606793.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2670..3953)
                     /locus_tag="OO566_003685"
     CDS             complement(2670..3953)
                     /locus_tag="OO566_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6606794.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4088..5158)
                     /locus_tag="OO566_003686"
     CDS             complement(4088..5158)
                     /locus_tag="OO566_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6606795.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(5136..5354)
                     /locus_tag="OO566_003687"
     CDS             complement(5136..5354)
                     /locus_tag="OO566_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6606796.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(5439..6062)
                     /locus_tag="OO566_003688"
     CDS             complement(5439..6062)
                     /locus_tag="OO566_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR6606797.1"
                     /translation="MTSQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            complement(6384..7091)
                     /locus_tag="OO566_003689"
     CDS             complement(6384..7091)
                     /locus_tag="OO566_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6606798.1"
                     /translation="MAQLYGTDVVRIRQNHHENKSRFVEGVHFYKVVGDELRALKHKV
                     ALNYFVKSAPNARHLILWTERGAARHAKMLETDQAWDVFEKLEDGYFNQREKVANHPQ
                     PVPQPESSLDRARAMEIALNVADRLVNMFPNLSSESKQAAVAGVFNPIVGRDVVPLPA
                     ISEHYYSATEVGQRYGISANKVGRIANTYMLKTEKYGKWFIDKSAHNDKQVETFRYNE
                     AGVHKIEELIEGERKAA"
     gene            complement(7039..7473)
                     /locus_tag="OO566_003690"
     CDS             complement(7039..7473)
                     /locus_tag="OO566_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6606799.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIGAVVRNGCCAHPAKPS"
     gene            complement(7546..7872)
                     /locus_tag="OO566_003691"
     CDS             complement(7546..7872)
                     /locus_tag="OO566_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6606800.1"
                     /translation="MPLHVQAYANAEFIAAFNPNVALALLDERERNLQYIKSRDQENE
                     EIALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAQTVTV
                     KEVGDA"
     gene            7934..>8081
                     /locus_tag="OO566_003692"
     CDS             7934..>8081
                     /locus_tag="OO566_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6606801.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDE"
BASE COUNT         1883 a         2006 c         1983 g         2209 t
ORIGIN      
        1 tactcactat ggcggcgcgg caggtccggt catctttggc gtgggttaca acgatattaa
       61 atcctggtgg ttggtcggtg cggtactgac gatattaacc ttcttggtgc atatcaccct
      121 cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg ctgtaaatat actcgtcata
      181 cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct
      241 cctggggatt cactcacttg tcgccttcct gcaactcgaa ttatttagag tatatccatt
      301 tattatcttt ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta
      361 tctgaaaaag gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc
      421 ggcgaaatta aaaaagaaga agcaaaaaaa ggcactattg cctggtctat tctctcttct
      481 cataatacgt ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac
      541 gatattacct ttgttggtat tgtacagacc gctaaagcgt ccggaatgga acgtttcccg
      601 ctgccgtatg tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg caccattaac
      661 ggtgatgacc atgtttttgg tttatcggca gctcagcgtt atggcggtat ttttgtgcct
      721 ccgcatattg cggtcatcca tcaatatatg cgtgagatga tggctggcgg gggcaaaatg
      781 atcctcgggt cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag
      841 ggtggcggtg agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc
      901 gtggttgcgg tgcatctgac cggaaaacct gcgccgtatg tggggccaca ggatgtggcg
      961 ctggctatca ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc
     1021 gttggaccgg gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc
     1081 acggaaacca cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg
     1141 gcgctgcacg gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac
     1201 gatggctgca tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac
     1261 ccgagcaacg tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag
     1321 attgaaattg agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa
     1381 gtggaaaatg gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac
     1441 tacgaaaacg tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc
     1501 ttctcgctgg cggtttaccc gtcatcacag ccggtgttta tggatctagc caaaaaaggt
     1561 gtggtagcag atttgattgg cgcaggcgca atcatcagaa ccgcattctg tggcccatgc
     1621 tttggcgcgg gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac
     1681 ttcccgaacc gcgaaggctc taagccagcc aatgggcaga tgtcagcggt ggcgctgatg
     1741 gacgctcgtt ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa
     1801 cttgattgct gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt
     1861 gtttatcagg gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt
     1921 aaagactggc cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag
     1981 atcctcgacg aagtcaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat
     2041 cgttcaaatc cgattggtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc
     2101 attaagcaga ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggcagcatg
     2161 gtctatgcgg tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt
     2221 gtgattggcg gtctggcgaa tattgccgag gagtacgcga ctaaacgcta ccgttctaac
     2281 gtcatcaact gggggatgtt accgctgcag atggcggaag tgccaacctt tgaagtgggg
     2341 gattacattt acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa
     2401 ggttatgtga tccatgaaga tgcgccggta acggaaatta cgctttacat ggaaagcctg
     2461 acggctgaag agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccat
     2521 cagatgtaaa aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat
     2581 gctggcgcat cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta
     2641 cgccgcatcc ggcaaatagt taatagctct tacttcttcg cctctgcaac cactttgctg
     2701 cccacgccgc ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc
     2761 tgtatttcgt cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc
     2821 accgcatcgg cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc
     2881 accacctgac cgttggtatt agcatcaaca tccagcgagc ggcccagttg cgccacgcca
     2941 tcaccggaag cattgaaacg gctgtttacg gcgaggaaac cgtaatagat gttagacagc
     3001 gtagccggtg caaacacata tgcttcttgc tgggtacgtg agttcaccac gcggaattcg
     3061 gtgttatcga acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatatag
     3121 ctgttggtca ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg
     3181 ttggtgacaa agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacctgg
     3241 tcaccgtcag tacgcagtgc taccgccgga tggttacctg catctacact atcgcccagc
     3301 gtgttttcga tggtcagatt ttgcagttgc agtccattgt tttgtgacca gaagaccgca
     3361 gaacacagaa caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac
     3421 gctggtttac ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg
     3481 ctcatgcccc catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg
     3541 tacagagtga ttccacctgg agcagcaggg acatacaccg ttccctgata ctcaccaggc
     3601 atcacggcaa tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc
     3661 tgaatcgtgg tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc
     3721 gcaggcaggg ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa
     3781 tagtgttgcg catcgaaatt cagcgcttct tttgccgaca gaatcgggcg agaagaggta
     3841 ccaggcgcag tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc
     3901 gtcacgccaa aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc
     3961 tccgggctat gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc
     4021 attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcattat cagtcaaaat
     4081 aaaatcatta tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg
     4141 atgccatggt gtccgacttg tgcccgagaa gatgttgagc aaacttatcg cttatctgct
     4201 tctcatagag tcttgcagac aaactgcgca actcgtgaaa ggtaggtgga tccccttcga
     4261 aggaaagacc tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa
     4321 gcggttcacg acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat
     4381 caagtgtttc cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa
     4441 tttttacgcc tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact
     4501 tcatttcgca taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc
     4561 tgagccaaca tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa
     4621 gtcttgatct ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta
     4681 tatggccttc agctattgcc tctcggaatg catcgctcag tgttgatctg attaacttgg
     4741 ctgatgccgc cttgccctcg tctatgtatc cattgagcat tgccgcaatt tcttttgtgg
     4801 tgatgtcttc aagtggagca tcaggcagcc ccctccttat tgctttaatt ttgctcatgt
     4861 aatttatgag tgtcttctgc ttgattcctc tgctggcgag gattttttcg tagcgatcaa
     4921 gccatgaatg taacgtaaca gaattatcac tgttgattct cgctgtcaga ggcttgtgtt
     4981 tgtgtcctga aaataactca atgttggcct gtatagcttc agtgattgcg attcgcctgt
     5041 ctcggcctaa tccaaactct ttacccgtcc ttgggtccct gtagcagtaa tatccattgt
     5101 ttcttatata aaggttaggg ggtaaatccc ggcgctcatg acttcgcctt cttcccattt
     5161 ctgatcctct tcaaaaggct acctgttact ggtcgattta agtcaacctt taccgctgat
     5221 tcgtggaaca aatactctct tccatcctta accggaggag ggaatatcct gcactcgcgt
     5281 acccatcgac gaactgtttc aaggctcctt gggcgtcgct ggcgtgcgtt ccactcctga
     5341 agtgtcaagt acatcgcaaa gtctccgcaa ttactcgcaa gggagcgagt attggtttat
     5401 tttctccaac aaaaaaggag ccgaagctcc tttgatgatt aaaattcgaa ttgtctcgcc
     5461 cgaaggcttt tcagcattgg ttttgcccgt tcgaaaaggg cgcttgcttg gtcaagtcgt
     5521 gtcgcctccc ttagcagtac atctctgttt ttcgtcaccg tgtaaaaagt ttcaaacgcg
     5581 atgtcataca gcttgctcgt gtatgatgag ttcagttcct tcaatatcgg gtacagacgt
     5641 ttgcagatgt cctgcgcatt ctccatctgt acctgcatat agcaaaggag gatgatttct
     5701 tcgtctgtga attgctgcgc tggttgcatg ctgcgaagtt tcttttcgca ctcgatgaag
     5761 tatcggcgta tctggcggcc tttttcgtta cgctcgacca tcgccgtttc tttggctgta
     5821 tcgagggtga ggtgatagtc tttgctacgg cgatcgcctc cacgtccttt gatccccctg
     5881 ttttggtgaa ccaaaatgta atcgacatca acggcaaagc cgtattctgt tatgcgattt
     5941 ttaatccatg tagagaagtc acgacgtact tccaaaaatt cgtgtaaatc acgggcatta
     6001 acgagaagag ttgtttcgtt ggatatagtg ccgttgaata cggggatgag ttgactggtc
     6061 atttttatgt cctttgcatt tgttcagata gccctgtgtt cagcagggcg gtcaggtact
     6121 tgaacaccgt gcaaagtcgg cccgtatcct taacctttcg gctgtttttc ggtatacgcg
     6181 ctacccgacc atatctgaaa aatggacata aaaaatccgc atgactgacg ggtgcggttt
     6241 ccgctttgca aggtgtgttc agcaccatga agcggaatat agccccgtta atgcggtgtt
     6301 gtcaaatcat gtaggcctta tcttgctgta agccgcgcca ttcgggcttt tccccacatt
     6361 tggggaaaac tatcttgtca aaatcatgca gcctttcgct ctccttcgat gagttcctct
     6421 attttgtgca ctccagcctc gttgtaccga aatgtttcaa cctgcttgtc attgtgcgcc
     6481 gatttgtcga tgaaccactt cccgtacttt tctgttttga gcatgtaggt gttggctatg
     6541 cgtcctacct tgttggctga aataccgtag cgctggccga cttccgtagc agagtagtag
     6601 tgctctgaga tagctggcag aggtacaaca tcgcgaccaa caatcgggtt gaacactcca
     6661 gctactgctg cctgctttga ttctgaggac agattcggga acatattcac cagtcgatcc
     6721 gccacgttca aagctatttc catggctcgc gcacggtcta ggctactttc tggttgcggt
     6781 actggttgag gatggttggc aaccttttcg cgctgattga agtagccgtc ctccagtttt
     6841 tcgaacacgt cccatgcctg atccgtttcg agcatcttcg cgtgacgggc ggctccgcgc
     6901 tctgtccaga ggatgaggtg gcgtgcattt ggggcacttt tcacaaagta gtttaaagct
     6961 actttgtgtt ttagcgcgcg cagctcgtca ccaacgacct tgtagaagtg taccccctca
     7021 acaaagcgcg acttgttttc atgatggttt tgccggatgc gcacaacatc cgttccgtac
     7081 aactgcgcca atgtttggtg ggtagttgtt tttaaagtca gctgctaaca catagcattg
     7141 tcgctctccc atttctggca ttcgctcact acagcttatc caggcatccg gagttaccgg
     7201 cactggctgg gcggtataaa gcggtgttat atctgcccga aaattacatg ctttatgcag
     7261 ccgcacccac cgttcgactt ctgctttgtc agaatacata ccagtgaacg tgttatattc
     7321 acggtcaatt tgcgtgaagg ttaccttcca cgccaccggc tctgctgtca gcgatgccag
     7381 agcaatttca taagcacggc gttcaacatt gtctcgcaca tccaaactgc tgatacgctc
     7441 tttgatttct ttaatcagtt ctttgtcgtt cacttatgcc tccgattccc ggatgtcacc
     7501 caaatcaacg ctacaatgcc aaccagtgca acgagcgcaa aactatcaag catctccaac
     7561 ctctttaacg gtaactgtct gtgcctccag ttctgctatg cgcttttctg aggcttcaag
     7621 taacgcctgc ttatcgcgta gcgcttcttc cagttcagca acatggcatt cactatcaat
     7681 aaggttgttc tctgctgctt ccagctcaac acgcaacttc cctaccgtta gcgcaatttc
     7741 ctcgttctcc tgatcgcggc ttttgatgta ttgcaggttt ctttcccgtt catccagtag
     7801 tgccagcgca acatttggat taaaagcagc aataaattca gcgtttgcat aagcctgaac
     7861 atgaagtggc acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
     7921 cacaggtgct ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg aatccgctca
     7981 actggttgtt gaccagaaat acacggtggc agatgccgcc aaagctatgg atgttggcct
     8041 ttccacaatg acaagatggg tcaaacaact gcgtgatgag c
//
LOCUS       DAJTXY010000157         8071 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTXY010000157 DAJTXY010000000
VERSION     DAJTXY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.157"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..1244)
                     /locus_tag="OO566_003693"
     CDS             complement(<1..1244)
                     /locus_tag="OO566_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6606802.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRNAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLG"
     gene            complement(1241..2119)
                     /locus_tag="OO566_003694"
     CDS             complement(1241..2119)
                     /locus_tag="OO566_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6606803.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2130..3037)
                     /locus_tag="OO566_003695"
                     /pseudo
     CDS             complement(2130..3037)
                     /locus_tag="OO566_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3024..3257)
                     /locus_tag="OO566_003696"
     CDS             complement(3024..3257)
                     /locus_tag="OO566_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606804.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3254..4111)
                     /locus_tag="OO566_003697"
     CDS             complement(3254..4111)
                     /locus_tag="OO566_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6606805.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4136..4465
                     /locus_tag="OO566_003698"
     CDS             4136..4465
                     /locus_tag="OO566_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6606806.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4455..4763
                     /locus_tag="OO566_003699"
     CDS             4455..4763
                     /locus_tag="OO566_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6606807.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4805..4981)
                     /locus_tag="OO566_003700"
     CDS             complement(4805..4981)
                     /locus_tag="OO566_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCR6606808.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4978..5388)
                     /locus_tag="OO566_003701"
     CDS             complement(4978..5388)
                     /locus_tag="OO566_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR6606809.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5385..5663)
                     /locus_tag="OO566_003702"
     CDS             complement(5385..5663)
                     /locus_tag="OO566_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606810.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5738..>8071)
                     /locus_tag="OO566_003703"
     CDS             complement(5738..>8071)
                     /locus_tag="OO566_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR6606811.1"
                     /translation="EPCLFGWQALDAGVRAVVLCEGEIDCMSYAQYGISALSVPFGGG
                     KGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASRLGEHRCRLVTLPYKDIN
                     ECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWESLAD
                     KFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPSTVT
                     GIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSETKR
                     HKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAELPD
                     YNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIGMLC
                     GLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTGVTYTYKDDKRHG
                     MDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAECLCIMVDHP
                     RHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATCCKMP
                     PVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKCGIGD
                     DDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITDLTDNL
                     FIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPLFLDE
                     QSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         1918 a         2199 c         1851 g         2103 t
ORIGIN      
        1 cccagagaac cgttacgttg tttcgtgata ttgatttctg cgatgcccct ggcctgcgta
       61 tccgggttgt acacttcatc cctgtaaagc atcagaatga tgtctgcatc cgcctctatt
      121 tctccggaat ttttcaggtc tgagttcatg ggacgtttat tgggtctgga ctccacaccg
      181 cgggagagct ggctcagcgc aatcaacgga aaaccaccgg attttgccag gcctttaagc
      241 ccctttgaga tttcacccac ggcaaggtca tgacgccccg tggttcgggt ttttatcagc
      301 ccgagataat caaccaccac cagcgccgtt tccggatatt taatcaggtg gtgtttcgtt
      361 gttgcgcata tctcatcaat ggccaggttc gcctggtcca ccatccagat attgcgcccg
      421 gtcatccgcc ccaccccttg tgagaaacgc gtccagtctt catcttcaaa gtgagccaca
      481 gatttcaggc gtgatactgg catccctcca gccgcagaca ccatacgttc accaatctgg
      541 atgttcgcca tctccatggt gaacagaagc acaccatgcc cctgctcagt caccttgtcg
      601 atgatgtcca gcgcaagttc ggttttcccc atcgaaggac gggcggcaat gaataccagg
      661 tctccgggct ccataccgcc tgtttttgcg tccagttcat caataccggt catcaacgtc
      721 ctggatttct ccagcccctg attccggcat tcaacacgct caaccacttc cggaagcaca
      781 tcatcaatat gtaccggctg aatgacgccc tttcctgtcg acagtgtgac catcatgttc
      841 tgcgcatcct tcagggcatc ttcagctgct tcacaggtat gcgcatcacg taatttctgc
      901 agcgcctcat tcagtgtttt ttctgcattg cgcagtgcgg cattgcgccg caacgctgca
      961 acatagtgct ccagtgaaga cttcacccag gttttacgcc cggtatcagt aatcaccggg
     1021 gcaagttccg gcatctcatt acacaacagc acggggtcaa tcactcctga aacacgggcc
     1081 tgtctgcaga tgcctgtata gatatcctga tacgctcgta cagaaaaaac gtccgctggc
     1141 agtgtggcca gaatatccat cacttcagga tctgctccgc gcagaaagaa cgcgccgatg
     1201 acagcgcctt caaggtcatc gttacgccat actggagttg tcatgcagcc acacctctga
     1261 tacgagaacg gtaactgggc cagttaaacg acaaccagtt gcgtcccccg tctgtgatcc
     1321 tgtcggcaat tcggggactg atgaacgccc acaactcttc cggtgagagg ttactgatca
     1381 gaatggtggg caagatactt tcgtacctgg cgttgataat ttcctgcaaa atagccattt
     1441 cagccgcgct gccaaactga acgccaactt cgtcgatgat cagcaaatcc attgacgcat
     1501 aacgctcaat aacttcatcc gctgtttttt cgctgtcatt ccgccagcaa tttttcacag
     1561 cacgggtaag gcgcatcacg tcggtgatct ccacactggc cagatagtta cggatgatgt
     1621 gttttgccat agccacagcc agatgatttt ttccggtacc acaactgccg gtcataacaa
     1681 gactggtacc gttctccagc atatctggcc aattctccgc atagcggcga caggccgcaa
     1741 gatttctggc tgcgccagga ttaacctcca gataattatc aaactcgcag tcccgaaaac
     1801 gcagggcaat tccggcgtta tcagtcagct cttccgcctt gatggacgac agctccatgg
     1861 tcaaatcgtt ggcctcagcg attaagcagt cagggcagca tgaaattttt tctctgtcct
     1921 cgccattacg atcgctccac accagtatat gcgtgtgata ttcgccatgt ttttcgcaat
     1981 atccgcgacc ttcacgcatc aggcaggaac gataaggcca tggcttttcg cccttctgag
     2041 caaatgcaat ctctgcccgt aactcatcca ttcgcgcctg tagtcttttt tgttgttcac
     2101 gcaggttaaa cgtcatcatc gctgtcacct cagaatgtca gtttgtcact ggatttaccg
     2161 aatttgtcag acatggctcc caggccagcc aggacatcga cctgtcgctg tcgcccacct
     2221 ccgggagcgg ctggctgttg ccagaagtct tcgaagtgac gatcgggtcc aaagaacgtc
     2281 gcagcctgct tcacgaactg tgtgccggta tttcctgtgg cacgtaccca ggcagcatag
     2341 cgtttcacgc catcaagcat ggcttcaggt tttattccct ccctgagccg ggctttccag
     2401 gctttgaagg ctgcggattt tgagttacca ccagcacgtt ttggatattc ctgccaggcc
     2461 tgttcaaatt ccggtgaata ttcctgtcgg gaagaacgcg ctggtgcaga cgcgtcagca
     2521 gatgcgccaa tagtgttttt actctctgta gtattctctg aagtaatctc tgttgtattc
     2581 tctgtaagat cgaaattggt tttcccttct ccgcggcgag gggtttcccg tgtccgcggt
     2641 gaaggctttc cctcctccgc gaaattgggt tttacagttt cccgaaaacg ggtttcccca
     2701 tttcgggaaa ctgattgttt tcattgataa tttcattaag gcgctcacaa tctatacggt
     2761 agaacatttt gtgctcaaga cgcttgttgg tttcaaccaa aatgcctctg gacacaagat
     2821 gcttacgcgc tacagcctgt tgttcaaatg taagtccggt ttcgtgttgt atctcttcac
     2881 gcgttttatg tacgccttcc gctgcatgtg ctttatccta ccagtaaaaa atctgaccaa
     2941 agaaaataac agcgtgcgga cttcccatgt atttaacgag cccagggtaa taagcaaccg
     3001 gatgtccaaa atcgagcaga agatcagacg gacgcatagc cacctcccag gcgtttaaac
     3061 atttttccgg gctgaaacgc caccagcgga taactcaggg tatgagtacg tccctgaacc
     3121 tggcagacaa ccttctggct ttcggtactg accaggcaaa cccgcagaac gtagccgttg
     3181 ctggtggtga accactgccc cacacggggg caatggttgt atcggtgata caggaaatta
     3241 acgatgtggc ggatcatggg agcacctcct tgtcagaacc attcagcctg gaatcaacaa
     3301 gtgcagcacc aaaaacagca tcacctacac ggtcgtacag tttgccagcc agcggagatt
     3361 caacggcctt aagcattggg taaagctggc ttgtccagat ttgatggatt tcacgcaaat
     3421 gcaggtatac gcctctggcg ttttgtgcga cagatgacat gtcagccgca ccagctcctg
     3481 ataagctcct ctccatctgg ttaaaggcat tgatgtatgc ctctttgaac cgggcggcac
     3541 gtttaccagt gaagcccata gcaaggaagg cgaagccgtc gcgggtaatt tggtagcagg
     3601 gaagtttgcg agtaccgccg ttgggctggc gtaccaaaat tgatgtctcc gcaaaattgc
     3661 gggcacaaaa ctctggagaa caatccaaaa tgcggatctt tttcagaaca tcgtcatgac
     3721 gtttagagaa gaagttggca acagccaggg atgaagtaac agcctgacca tcaacgatgg
     3781 caatttcagg ttgagagagg gttgggagag tagtcatggt gacagcccct atgttgaatt
     3841 caaagaactc accacatggg acgccaatca cagaggtggt gagacgtaca gggttggcgt
     3901 aaccggtcaa catagaaccc ggcgcatctt gcgatgcccc tgcacgcccc accataattt
     3961 gggcgtagca atgctcatga cacgaaaaaa ccgcatgagc gcggttatgc tctatattga
     4021 atttcaggac gccaatcccg gcacccgctt tataaggtgc agagacagtg taacgtcccg
     4081 aaattgcaga atcaatatat tgtgcagata taacgatttt gttatgtttt gctgtatgaa
     4141 ctacactatc gaatactaca gtgaagaggt ccgactggag gtcgatcagc ttccattgag
     4201 tatgcgtgcc agataccagc atcttgttga acgcatgaag gtatacggca gcaatctcgg
     4261 agaacctcac accagcgcct ttggtgacgg gcttttcgaa ctcagaatta aaggcaacga
     4321 tgggatcgcg cgtgtttttt actgcactct gacagggaaa cgcatcatca tgctgcatag
     4381 ttttgtaaag aaaacgcaga aaacaccgcc agccgaacgc aagaaagctg aaaccagaat
     4441 gaaggaggtt aagcatgact ggtaaacgca ctctccccac catgacacac gacgaaatgg
     4501 cagccaaatg gatggaagac ccggctttta aagcagaata cgacgctatc gctgacgaat
     4561 tcgcactgct tgatgaaatg ctggcagcac gcaaagaagc tggcttaact caggctgaag
     4621 ttgccgagcg aatgggaaca aaagcgaccg cgatcaccag aatggaaagt aatctcgcat
     4681 caggtatcag cggcccatca tttgccacac taaaaaaatt cgcccgcgct actggaaaaa
     4741 aactccagat ccgcttcgtt taaccacacc gcgccgtcat tctggcggcg caccggataa
     4801 atagtcattt cctcgcacga tgtcttagcc accggatatc ccacaggtga gccgtgtagt
     4861 tgaaggtttt tacgtcagat tcttttggga ttggcttgcg tttatttctg gagcgtttcg
     4921 ttggaaggta tttgcagttt tcgcagatga tgtcggtgaa acttcgtcgc tgtcgcctca
     4981 ttcatacctc ctgtcggtaa atctgacacc ctgaccaata gcccaggctg ttgtgtactc
     5041 gatcagactt gccatgcgct tcacgctcat ctgcgcgctg ctttcgcgaa tgttgacgca
     5101 ttctccttca aggccgggca aaacatcagc ttcctgtttt gttgccactg catgaccgct
     5161 gatcaacaaa accttccatt gttctggttt taaccatttg ccgcaccatt gaacctgacg
     5221 agcgatatcc gccagcatcg cgtgaaattt tgcgttctgg tcaagatttc gcttgtagtc
     5281 agtaatgcgg atggtgactg gcttgtcttt atcgagagga gttgagagga tggcatttat
     5341 tgcggcttgc tgttgttgct tagttcggag gaagattgtt tgcttcatcg aaaattcttc
     5401 tctttaattc cagcggctct gatagctttc attactgcaa ttaccgtttt gtccttccca
     5461 tcctcatgcc ccatcgcata agcaccttct tcaccatctt tccaaaagtc gtcattcgat
     5521 tcgggccagt cgatatccag ttcaatagct gctcgcggtg cctgccatat cacccaggca
     5581 aactctttta attcatcgtc tcctgtgaac tggcttttgt cttttgacca ccagttttca
     5641 aactgtcggt agctatcgtt cacttccctc tcccccaaat aaaaaggcct gcgattacca
     5701 gcaggcctgc tattagctca gtgatgtaga tggtcattta atactccgtc acgttttcct
     5761 gtcgccacgc ctcgtcatat tccgatttcg gcatattggc gatgtagcta tatggcgatc
     5821 ctgattcaag ttgcaggaac tggtgcgatt gctcgtcaag gaacaacggg acaccacctt
     5881 cccaaccttc gccgttacgt tgtttttcaa gcatcaaaac agatgccgga gatgccagta
     5941 gctgttcgtc cttctctgac atcttttcac cactctgaac tctctgtaac gctctctcgc
     6001 gagccttgtt acgccagatg atgaaaaggt tgtctgtcag gtctgttatc gctccagagc
     6061 cttttacgtc cattttcccg gttggttttt cttcgctgtc tccttttcgc gagtgagtaa
     6121 cgagaatgac gtgggagttt gttttgtttt tgaagtcgca aatcgagtca acaaacgcct
     6181 tctgcccgtt atagtcatcg tcgcctatgc cacatttcat caggctgtcg atgatgaata
     6241 actggatccc gtatcggcgg cgagcgtagt cgaatatttc gatcagcctg tcggctttcg
     6301 ccgttccagt caggccaaac acccaaagtc tttcgtcata aaatttaaat gcagagtcaa
     6361 tttccagcac tggcggcatc ttgcagcacg ttgcctgacg ggtaagtcgc ttaaggagaa
     6421 taccaggctt cagctcaagt gacgcgatgc acgtcttcac accctgacgc attgcctcaa
     6481 gtgccatatg cccgacaacc tcggtgttat gcgtcgccac atatcccctg gttacataca
     6541 ggtggcgagg gtgatcgacc ataatacaca agcattctgc attgccgatt ttttcaacat
     6601 tcctgacgaa taccccacag ccctcgaatc gatgcgcagt taatctgcca ttgagtcgtg
     6661 gtgaacggat ggcctccctg atttctcttg tcagtctgat tcttgcctcg tatgaatcca
     6721 tcccatgccg cttatcgtcc ttgtatgtgt atgtcacgcc agttttaact cgcgtccggc
     6781 atgacccgcc gagtgagtta accagttgaa caaccccatt gcgcagttct tcacttgctg
     6841 aggaaaaaac aagcgttcca tccttctcga catacccatc cgtttcaagc agaccacaca
     6901 gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac acgagggatg aatttgtttt
     6961 ttgctgtgca ccccattagt ccataacctc gcagggtctc catgagtgga tttacctgac
     7021 cacgcgccgt tgatatcagc cagtccttac catctccaga gaagttgtaa tcaggcagtt
     7081 cagccttcat acgctcgatc atgtatggct cgacgtttga aaacttcacg ctcccattgc
     7141 taagactacc atccccgaga agggagccga taacccatgc taatggctct gagtggtcgc
     7201 caaagtcacc agttatttca ggaatcctaa cgccattttt gtgcctcttc gtttcactca
     7261 accgcttcaa cccgaaggta tctatcacgc ggcgtttctc gcctttcgtg aatccacgac
     7321 tagtgacctc ccataggtga tcgcctgcgc aatcaacata acgaccatct tcaaatgtga
     7381 ctcggtaaac atctctaaca ccctgcggga atatcccagt gacagttgac ggattgccgt
     7441 ctactgacgc cacctgatcg ccaattttta cattcccgtg agtagtccat gtcccatcag
     7501 ctaaaagaat aggctcattc agtgggcatg cctttccgtg accgttcaca ccattgacca
     7561 gcgtcaactc tgcctcacgg aactggaatt tatctgccag agattcccac ggtggattaa
     7621 acagatactg ctgcttgccg tagaaagcgt tgatagtgtc ctggtaaaac tctcgcgcac
     7681 tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat gtactgccag atttcatcct
     7741 cggtaacacc gttcatcagg cattcgttga tgtctttgta cggcagagta acaagacggc
     7801 aacgatgttc accgagtcgg cttgcgattt cccttgcggc ttcacgacca acatcatcaa
     7861 cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt atgatactca aactcaatcc
     7921 actgttgctt agcgcctttc ccgccaccaa acggcacgga taacgccgag atgccgtatt
     7981 gcgcatagct catacaatca atttcgcctt cgcaaagcac aaccgccctc acgccagcgt
     8041 ccagagcctg ccatccgaac aggcaaggtt c
//
LOCUS       DAJTXY010000158         7944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTXY010000158 DAJTXY010000000
VERSION     DAJTXY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.158"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..554
                     /locus_tag="OO566_003704"
                     /pseudo
     CDS             1..554
                     /locus_tag="OO566_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
     gene            555..1658
                     /locus_tag="OO566_003705"
                     /pseudo
     CDS             555..1658
                     /locus_tag="OO566_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            1703..2026
                     /locus_tag="OO566_003706"
     CDS             1703..2026
                     /locus_tag="OO566_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR6606812.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            2023..2439
                     /locus_tag="OO566_003707"
     CDS             2023..2439
                     /locus_tag="OO566_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6606813.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            2405..2929
                     /locus_tag="OO566_003708"
     CDS             2405..2929
                     /locus_tag="OO566_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606814.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            2926..3489
                     /locus_tag="OO566_003709"
     CDS             2926..3489
                     /locus_tag="OO566_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606815.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLSNERDEKGQWAAYDAVHDVRRELWKALLG
                     WMPDPQGGEIVYVGGTLLDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLS
                     IDVDYIDPGTGPDGDIEHHLEMRFPQN"
     gene            3499..3660
                     /locus_tag="OO566_003710"
     CDS             3499..3660
                     /locus_tag="OO566_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6606816.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            3657..5153
                     /locus_tag="OO566_003711"
     CDS             3657..5153
                     /locus_tag="OO566_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6606817.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAH
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            5153..5509
                     /locus_tag="OO566_003712"
     CDS             5153..5509
                     /locus_tag="OO566_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6606818.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            5509..5845
                     /locus_tag="OO566_003713"
                     /pseudo
     CDS             5509..5845
                     /locus_tag="OO566_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            5930..7879
                     /locus_tag="OO566_003714"
     CDS             5930..7879
                     /locus_tag="OO566_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6606819.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
BASE COUNT         1973 a         1814 c         2359 g         1798 t
ORIGIN      
        1 atggctccgt ctttggtgta aaggacagcc acgatgatgt ggtgatgtcc ggggcatttg
       61 ctgcttccct gcgggcgtgg agtgacagaa aagcgttacc tgcgctgctc tggcagcacc
      121 gcatggatga acccatcggt gtttacaccg aaatgaagga agacgatgtc gggctttacg
      181 tcaggggacg gttgcttatt gatgatgatc ccctcgcaaa acgtgcacat gcacacatga
      241 aggccggttc gttaaccggc ctttctattg ggtacgtcct gaaagactgg gaatacgacc
      301 ggagcaaaga agcctttctg ctgaaagaaa tcgacctctg ggaagtcagt ctggtgacgt
      361 tcccgtctaa cgacgaggcg cggatcagcg acgtcaagaa cgcactggcc cgcggggaaa
      421 tccccgaaca gaaaaaaatc gaaagagtcc tgcgtgatgt cggactctcc cgtacccagg
      481 ccaaagcatt catggccggg ggctatggcg cactgtccct gcgcgacgct gaggatgtgg
      541 gctctgcact gaacggagaa gtggaaaccc tcaacggaaa actgactgaa ctggaaaatc
      601 tcaaaagcga tcttgaggct gagctggctg aagttaagcg tccggcaggt ggtacgcaaa
      661 ataaagttgc cggggaacat aaagaagcgt ttatcggttt tatgcgcaag gggcgtgaag
      721 atggcctgcg tgagcttgag cgaaaggcac tgcaggtggg taatgatgaa gatggtggtt
      781 atgccattcc ggaagaactg gatcgcacca ttctgacgtt gctgaaagat gaagtggtga
      841 tgcgccagga ggccaccgta atcactctga gtggctcgga ttataaaaaa ctggtgaatc
      901 tgggcggcac gacgtccgga tgggtggggg aaacggaagc gcgtccggaa accgccacct
      961 caaaactggg actgattgag ccctttatgg gggaaatcta cggcaacccg caggccacac
     1021 agaaaatgct cgatgatgct ttctttaatg tggaagactg gatcaacagt gagctggcgc
     1081 tggaatttgc cgaacaggaa gaaattgcct ttaccagtgg cgacggtagc aaaaaaccaa
     1141 aaggttttct ggcttatgag tccaccgatg aagatgacaa gacccgtgcg tttggcaaac
     1201 ttcagcacat tgcttccggc gcggcttccg gcgtgaccgc tgatgcgatc attaaactga
     1261 tttacacctt gcgtaaggcg caccgcagcg gcgcgaagtt catgatgaac aacagcagcc
     1321 tgtttgccat tcgtctgctg aaggataacg acggaaatta tttgtggcgt ccgggtattg
     1381 agctgggtca gccttcttct ctggcagggt atggcatcgt tgagaatgag cagatgccgg
     1441 atattgccgc cgatgcaaaa gccattgcgt ttgggaactt caaacgcggc tataccatcg
     1501 ttgatcgcat cggtacccgt atcctgcgtg acccgtacac caacaaaccg ttcgtgggtt
     1561 tttataccac caagcgaacc ggcggtatgc tggtggattc tcaggcgatt aagctgatga
     1621 aaattggggc tgcaacccgc cagaaagccg ctgcgtaatg cggttttttg tgcccgcgta
     1681 atcgcgggca caggaggaaa atatgctcct gaaagaagag gagattaaat ctcatctccg
     1741 gctcgatgat ggtttgtaca gtgacggcga ttttctgaaa ctgctggcac aggcggtaca
     1801 gaaaaaaaca gagacatacc tgaacaggaa gttgtatgca ccggaagaga cgattccgga
     1861 agatgatcct gacgggatgc atctgactga tgacgttcgt ctggcaatgc tgatgctggt
     1921 cagtcatttt tatgaaaatc gctcaacgat caccgatgtg gagaaactgg aaacgccaat
     1981 gagtttcaga tggcttgctg gcccttacag gattgtcccg ctatgaaaat caggcaaagt
     2041 cagaccagtg ccacttacct tttaccggat cccggagagc tgaatcgccg gataaagatc
     2101 cgccttcgtg tggatgaacc caccgctgat tttggtacgg agccaacgta tccggagtcg
     2161 tttgatgtct gggcaaaggt ggctcagcca ggcgctgccg cttatcaggg ctcagtgcag
     2221 acagaaaata tcgttacgca ttattttacg atccgtttcc ggcacgacat cacggcagat
     2281 cacgaagtgg tttattacgg ccaagagtac cggatccggc gaatacgtga cctgaacggt
     2341 cagcggcgtt ttttattact ggaatgtgaa gaactgcgta cagcgcgacg acggggtgaa
     2401 tgccatgaat cagacagcat ttttacacgt cgactttaaa caaccagagg agctggagtt
     2461 taatcgcgcc cgtctgcgtc gggcgtttgt gcaaatcggg cgcgtttaca tgcgtgatgc
     2521 cagacgtctg gtgattaaac gcggacgctc cggtccggga gagaacccgg gatatcagac
     2581 cgggcgtctg gcccgctcca ttggttatta cgttcccaaa aaaacgacgc gtcgccccgg
     2641 actgatggtg aaaatttccc ctgaccagaa aaacgggcag gggaatcgcc gttttcctga
     2701 aggtgctcct tattatccgg cgtttctgta ttacggcgtt cgccattcag cgtatggaat
     2761 ggataaaaag gataagcggc agaagaagca ccattcttcg actttccggc tggccccgcg
     2821 taataacttt atggctgacg ttattgagcg tcagcgtcac tggacgcagg aactgttgtc
     2881 cagtgagctg cagcgttcgt tacgtccggt aaaaaggaag cataaatgaa actgacaacg
     2941 ataattgcgg cgctgcgtga gcgatgcccg cgttttgagg agcgtgttgg tggcgcggcg
     3001 cagtttaagg cgatccctga tgccggaaaa cttcgcctgc ctgctgccta tgtggttccc
     3061 tctgacgatg cgccggggga acaaaaatca cagaccgatt actggcagga tttgactgaa
     3121 ggcttctccg tgattgtggt gctcagcaat gagcgtgatg aaaaagggca gtgggcagcc
     3181 tatgacgccg ttcatgatgt ccggagagaa ctctggaaag ccctgcttgg atggatgccg
     3241 gacccgcaag ggggcgagat tgtttatgtc ggtggcaccc tgctggatct gaaccgttac
     3301 gaactgtatt accagtttga ttttacggcg aagtatgaaa tcacggaaga agacacgcga
     3361 caggcagagg acgtgaatgc cctgccggat ttatccctgc tgagtattga tgtggattac
     3421 atcgatcctg gtactggccc ggatggtgac attgagcacc atctggaaat gcgtttccct
     3481 cagaattaag agtccctcat gtttgtgaaa cccctgaaag ggcggtcggt tcctgaccct
     3541 gcccgtggag accttttgcc ttccgaaggg cgaaatgtgg aagaaagcag ctactggttc
     3601 cgccgtatag cggcgggtga tgtggtacgt gttaaacagg ataaggctaa aaaatcatga
     3661 cgataagttt taatactgtt ccgtcgaata cgctggtgcc gttgttctac gccgagatga
     3721 acaattctgc ggcaaataca gcggtgacca gcgcgcctgc attactgatc gggcatgcca
     3781 gcaacgatgc agccattgag gttaacagcc tggtgctgat gccatcggca gattatgccc
     3841 gccagatttg tggggcgggg agccagctgg cgcgtatggt ggaagtctac cgtcagacag
     3901 atcctttcgg tgaactgtat gttattgcag taccggaagc cagaggggcg gcggcgacgg
     3961 tcagggtgac ggttgccgga gaagcagagg aaagcggcac cctgagtctg tatgtcgggc
     4021 gctcccgtgt acaggtgcct gtggtgaatg gcgatgatgc cactgcggtt gccaccgcga
     4081 ttaaggaagc ggtaaacggg gttatcaccc tgccgtttac ggcgtcatct gatgcaggcg
     4141 tggtgacgct gactgcccgt cataaggggc tgtatggtaa tgagttaccg gtctgtctga
     4201 attattatgg ttctggtggt ggtgagattc tgccagcagg gcttcgggtg gtgacggaag
     4261 tcggtgctgc cggtagcgga gcgcctgatc ttaccgccgc tgttgctgct atgggcgatg
     4321 aggcattcga ctttatcggt ctgccgttca acgatgccgc ctccatcaat atgatgatga
     4381 ccgaaatgaa tgacagcagc ggtcgctgga gttatgcgcg ccagttatac gggcatgtct
     4441 ataccgcaaa actgggaacg ctgtcagagc tggttggtgc cggagatatg cataatcagc
     4501 aacatatcac gcttgccggt tacgaaaaag aaacccagtc gcctgtcgat gaactggttg
     4561 ccagtcgcct tgcccgtgaa gcggtattta tccggaatga tcctgcccgt ccgacacaga
     4621 cgggggagct ggtggggatg cttccggcac cgaaaggtaa gcgattcatc atgacagagc
     4681 agcagaccct tttatctcac ggcgtggcga cggcttatgt ggaaggcggc acgttgcaga
     4741 tccagcgttc cgtaaccacc tacaaaaaga atgcgtatgg cgtggcagac aacagctatc
     4801 tggacagtga aactctgcat accagtgcac atgttcttcg caaactgaaa tcggtcatca
     4861 caagcaagta cggacgtcac aagctggcaa atgatggtac ccgttttggt ccggggcagg
     4921 cgattgtcac tcctgccgtt atcaaagggg aacttctggc gacatatcgt cagatggagc
     4981 gtgccggtat tgtggaaaat tacgatctgt ttaaacagta tctgatagtt gagcgtgatg
     5041 cagataaccc gaaccgactg aatacgctgt tcccgccgga ttatgttaat cagttacgtg
     5101 tctttgcggt ggttaaccag ttccgtcttc agtattcaga ggagtcagca taatggcaaa
     5161 gatcgccgga acctgttttt ttaaagtgga tggtcagcag ttatcgctga caggtggcat
     5221 tgaagtgccg atgaacacca atgtccggga tgacatcgtc ggcatggcag gggatgtgga
     5281 ttacaaggag acctggcggt caccttacgt taagggcacg tttaaagtgc ccaaaaactt
     5341 tccggtcgac aaaattacca cctcagacca gatgacgatt accgctgagc tggcaaacgg
     5401 catggtgtat gtgctttcgg cggcatggct gcacggagag gctaatcata atgccgaaga
     5461 aggcacggca gatcttgaat tccacggcga agagggaggg tatcagtaat ggcaatgaac
     5521 gttacagaaa tagttttaaa aaaaccggtg acagcgcata acgaaatgct gcatgtgctg
     5581 gagttgcgcg agcccacgta tgacgaaatc gaggcgctgg gttttccttt cattatttcc
     5641 ggtgaaggca gtattaaact ggacagccag gtggcactga aatatatccc gttgctggcg
     5701 gggatcccgc gttcatcggc ggcgcagatg gcaaaactgg atatttttaa gaccagcatg
     5761 cagatcctgc gtttttttac ccagtcggag acgggaagca cctccgggaa acgactctac
     5821 aatgttgcct ggttctggaa actgaatcca ctggagctgc gacgggtggc tgtttcgcag
     5881 tttacagaac tggaagccga ggccgtccgc attaacgagg agatgaagca tggctgacag
     5941 ttttcagctg aaggcgatca tcactgccgt ggataaggta tcggcaccgc tgaagggaat
     6001 gcagcgccag ctgaaaggtt ttaaaaagga gtttgccagc ctgtcactgg gcgcagcggg
     6061 tgccggaacc gcagtactgg gggcgctggc gctcccggtc aaatctgcca ttgcccttga
     6121 atcaaaaatg gcggatgtcc ggaaagtggt ggatggtctg gatacgccgg aagcgtttaa
     6181 ggcaatgacg gagcaggtgc gcgacctgtc aacagaactg cccatgtcgg cggaaggtat
     6241 cgccgaaatt gtggcggcgg gtggtcaggc tggtatcgcc cgtgacgagc tgatgcagtt
     6301 tactgacgat gccgtgaaga tgggcgtggc ctttgacgcc acggcagaag aatccggtca
     6361 gatgatggcg cagtggcgca ctgcctttaa actgacacag ggagaggtgg cagggcttgc
     6421 ggataagatt aactatcttg gcaataccgg tcctgcaagt gcgaaaaaga tttctgatgt
     6481 tgtgacccgt attggccctt taggcagtgt tgcgggtgtg gcctccggag agattgccgc
     6541 aatgggggca accattgccg gaatgggggt ggagtcagaa attgctgcga cggggataaa
     6601 gaattttatg ttgtcgctga cagcgaggga ttctgccacc aaatcgcaga aaaaagtgct
     6661 ccgctcgttg cgcattagcc cgaaaaaact ggcggcggat atgcagaaag acgcccgcgg
     6721 ggccatgctg cacgtactgg attctctggc aaaagtgccg aaagaaaaac aggctgctgt
     6781 gctgaaagcg ctgttcggga aggaatccct gggggccatt gcgccgctgc tcacgaatct
     6841 ggatttgttg cgaaccaact ttaatcgtgt ggcggatgcg cagcagtatg gcggctcaat
     6901 gcagaaagaa tatgccgccc gtgccgcgac gacagaaaat cagttgttgc tgctgcagaa
     6961 ccagatcaat gcgatttctt ccacgctggg ggaaaccttc ctgccttcac tcaatgaagg
     7021 cataaaagag atgaagcctt ttctggaaga agtgcgcacg tttgtcagag aaaatcctga
     7081 ggtcgtaaaa accatcgcga aaactggtgc ggcattactg acgatgggcg tggcgattgg
     7141 cacattgaca cgcataacaa aaatcatggg tagcgtgatg aatatgacgc cggcaaaggg
     7201 attaattgcg cttctcgtcg gtggtgcata tctcataatt gataactggg aaacggtcgg
     7261 tccggttgtg aaaaaagtct ggcaggaggt tgatcaggtt gtcagagcaa tggggggatg
     7321 ggaacaggca gtaaaaacaa tagcaacggt ttctgccctg tatattggtg tgaaggcggt
     7381 ggcaagtatt cgtgcggcaa cagtcgccca gaatcaatgg acaacggcgg caggaaaaac
     7441 agggctgaaa ttaaagggcc tcggaaagat cagtctgatt ggtggattgc ttgaactggg
     7501 catgatggcg caggagtttg aaaaagaaca tccctggctt gttaaaaatt ttgtggctga
     7561 tgcactgaac agtggatttg gtctgaatga taagttcgac gagtggggca aacagttcca
     7621 tgattttgtc tatgacatga ccggatggca gatgccacgt ggtgatgggt atttatctcc
     7681 cgataaacgc tacaccccga atgtatcact ggaaaggaat cagctgttaa gccttgcatc
     7741 ttcaccggca acccgcagcg aacttaaggt gacgtttgac aacgcgccgc ctggaatgcg
     7801 cgttatcgat ttgccgaaaa caggcgatcc ctttatgaaa atcacccatg atgttgggta
     7861 ttctccattt aaaaggtgag ttcttttttt gtggtgaaag atgaggcaaa attatcgcgt
     7921 aacatgctaa caatggagtt tgtt
//
LOCUS       DAJTXY010000159         7855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTXY010000159 DAJTXY010000000
VERSION     DAJTXY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.159"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="OO566_003715"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="OO566_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR6606820.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /gene="dcuS"
                     /locus_tag="OO566_003716"
     CDS             323..1954
                     /gene="dcuS"
                     /locus_tag="OO566_003716"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6606821.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="OO566_003717"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="OO566_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6606822.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="OO566_003718"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="OO566_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6606823.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="OO566_003719"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="OO566_003719"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6606824.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAVIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6422..7051
                     /locus_tag="OO566_003720"
     CDS             6422..7051
                     /locus_tag="OO566_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6606825.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7190..>7855)
                     /locus_tag="OO566_003721"
     CDS             complement(7190..>7855)
                     /locus_tag="OO566_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6606826.1"
                     /translation="FAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRR
                     ILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVD
                     YGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQF
                     IMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSD
                     VKA"
BASE COUNT         2033 a         2004 c         1930 g         1888 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgacttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc taggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtaacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctccgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc ggtcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaccaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaattgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc acctggcagc atgctgccag gtgctgggcc acctcttttg
     6361 cgattgtaat ttcatgcttg ctggtgaata gtcagtattt tccctgactt gcgaactcac
     6421 catgacccgc acactcaagc cgttaattct taacaccggc gcactggcgc taacgttaat
     6481 cctgatttat accggcattt cggcccatga caaactcacc tggctgctgg aagtgacacc
     6541 ggtgattatc gtcgtgccgc tactacttgc caccgccaga cgttacccgt taacgctgct
     6601 gctctatacg ctcatcttct ttcacgccat catcctgatg gtaggcgggc agtacaccta
     6661 cgcgaaagtc cccgttggtt ttgaggtgca ggaatggctg gggttgagtc gtaatccgta
     6721 tgacaagctg gggcactttt tccaggggct ggtgcctgca ctggtggcac gagaaattct
     6781 cgttcgcggg atgtacgtgc gcggacgtaa aatggtggcg tttctggtct gttgcgtggc
     6841 gctggcgata agcgcgatgt atgaattaat cgagtggtgg gcggcactgg cgatgggcca
     6901 gggagcggat gattttctcg gtacccaggg cgaccagtgg gacacgcaat ccgatatgtt
     6961 ctgcgcgctg cttggcgcat taacgacagt gatattgctc gctcgctttc attgccgcca
     7021 gctacggcgc tatggcttga taaccggata acaacgtgca ctttgttgat gccggatgcg
     7081 gcgtgaattc cttatccggt ctacaaaatt tgcaaatcca aagcattaca ttaagacgtg
     7141 gcctgataaa catatcgcat caggccattt tcgttagcta ttgtccacgt caggctttca
     7201 catcactcac tgcaccgacc ggaatatcag catgaacagg ctcgggcggt actttgcgat
     7261 atttatccag cagatgaatc tggatcctgc gcagcgtgtc gccattgagg cgatagaaac
     7321 ggaagtagag aatcagcgtt accatgaaaa acagagtcgg tagagcaatc ataataaact
     7381 gcatacctaa tagggcttgc gtagactgtt caacattcgg tacatagcca atcatcccta
     7441 acacaaccgc aatgaaaaaa gccgcaaagg ctgaaccgcc cttcaccacc atagtctgca
     7501 cggagtaagc gatgctttca cagcgtacgt gcagtttata ttcaccgtaa tcaacggtat
     7561 ctgccaccat gatgacctgt aatacccaga aaagcgccgt tcccacattc agcaaaatac
     7621 ccgcaatcac aatgaggacg acgttgtgat agctcattaa tgccattaac aggagaacac
     7681 cacagcttaa caccggaaga atagatgctc cggcccataa aatgcgtcgg gataatgatt
     7741 taactaagcg tgggaagaat actaacgtca ccaggttagc agctcccgca tacgacagat
     7801 aataggggaa caaatccgca tcaccgataa catatgagaa ataatagata gcaaa
//
LOCUS       DAJTXY010000160         7473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTXY010000160 DAJTXY010000000
VERSION     DAJTXY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.160"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            15..1073
                     /locus_tag="OO566_003722"
     CDS             15..1073
                     /locus_tag="OO566_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6606827.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1116..1619
                     /gene="yeaK"
                     /locus_tag="OO566_003723"
     CDS             1116..1619
                     /gene="yeaK"
                     /locus_tag="OO566_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6606828.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVIHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1620..1724)
                     /locus_tag="OO566_003724"
     CDS             complement(1620..1724)
                     /locus_tag="OO566_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606829.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1894..2340
                     /locus_tag="OO566_003725"
     CDS             1894..2340
                     /locus_tag="OO566_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6606830.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2297..3118)
                     /gene="nimR"
                     /locus_tag="OO566_003726"
     CDS             complement(2297..3118)
                     /gene="nimR"
                     /locus_tag="OO566_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR6606831.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3215..4396
                     /gene="nimT"
                     /locus_tag="OO566_003727"
                     /pseudo
     CDS             3215..4396
                     /gene="nimT"
                     /locus_tag="OO566_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4451..4798
                     /locus_tag="OO566_003728"
     CDS             4451..4798
                     /locus_tag="OO566_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6606832.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="OO566_003729"
     CDS             complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="OO566_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6606833.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5257..6282
                     /gene="dgcP"
                     /locus_tag="OO566_003730"
     CDS             5257..6282
                     /gene="dgcP"
                     /locus_tag="OO566_003730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6606834.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6315..6413
                     /locus_tag="OO566_003731"
     CDS             6315..6413
                     /locus_tag="OO566_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6606835.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6416..6490
                     /gene="yoaJ"
                     /locus_tag="OO566_003732"
     CDS             6416..6490
                     /gene="yoaJ"
                     /locus_tag="OO566_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6606836.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6542..6973)
                     /locus_tag="OO566_003733"
     CDS             complement(6542..6973)
                     /locus_tag="OO566_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606837.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKALEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(6967..7194)
                     /locus_tag="OO566_003734"
     CDS             complement(6967..7194)
                     /locus_tag="OO566_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6606838.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         1834 a         1795 c         1806 g         2038 t
ORIGIN      
        1 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
       61 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      121 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      181 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatag
      241 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      301 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      361 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      421 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      481 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      541 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      601 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      661 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      721 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      781 tccggatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
      841 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
      901 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
      961 atgacaacct cggtcatcag gagggggatt tagcgattac gttacatgat gcgtataaag
     1021 cttccgatga gcgtttatat gtcaataagc agaacaaaaa cagccgttca tgataacctt
     1081 ctgtggttgt ttgcttgtaa tctcaggagc gtgaaatgac tgaaatggct aaaggaagcg
     1141 tgacgcatca gcagttaatc gctttattat cacaagaagg tgctaacttc cgcgtcgtta
     1201 tccatgaagc ggtaggtaaa tgtgaagcgg tgtcagaaat tcgtggcacc gctctaggcc
     1261 agggtgctaa ggcactggta tgtaaagtca aaggcaatgg cgtaaatcaa catgttctgg
     1321 cgatcctcgc agccgatcaa caggccgatc tgagccaact cgccagccat ataggcggat
     1381 tacgcgcttc gttggccagc ccggcggaag ttgatgaact cacaggctgt gtcttcggcg
     1441 cgatcccccc tttcagcttc catccaaaac tcaaactggt tgccgaccct ctactcttcg
     1501 aacgatttga tgaaatcgcc ttcaatgcag ggatgctgga taaatccgtt attttgaaaa
     1561 ccgcggatta tttgcgcatt gcgcaaccag aactggtcaa tttccgccgc actgcgtagc
     1621 tagcctatcc gttcaataag cagaacggac aaaaccagca caaccgcaat agcaaaaaac
     1681 gatgatgtga taatcagtgt ctcgacgaac atttgatcgt tcatagcatg cccccgggag
     1741 atagctgttt tcacgttatt gttagcgtgc acaaatggca gtttgatgac agttcgcctg
     1801 attttttatg caaaaaacgt aaagattttc tacttccttc ctgcagcaag cgtaaagtaa
     1861 gcaggcttat tattttttgg caaggaaacc acgatgtttg atgtcacttt gctgatcctg
     1921 ctcgggttag ctgcgctggg ctttatcagt cataacacca ctgtcgccgt ttcaattctg
     1981 gtgttgatca ttgtccgcgt cacaccgtta agcacctttt tcccgtggat tgaaaaacag
     2041 gggctaagta tcgggataat cattctgacg attggcgtta tggcccccat cgccagcggg
     2101 acgctaccgc cctcaacctt gattcactcg tttctgaact ggaaatcgct ggtggcaatt
     2161 gcagtaggcg ttattgtctc ctggctgggt gggcgcggcg tgacgttaat gggcagccag
     2221 ccgcaactgg ttgctgggct gctggtgggc actgttttag gcgtagcgct gtttcgcggc
     2281 gtaccggttg gcccgcttat tgccgcgggt ctggtttcgc tgattgtggg gaaacagtag
     2341 ttaatcccgc aatatatcgg cctggcgttt gccctaggcc tttcttaaac atagtaataa
     2401 aggccgttgt tgaatcatac cccagcgtat gagcaacttt ctgtacagta tcgcctttca
     2461 ctaatccctg aagcgccata atcagctgta actgctgacg ccactggcga aaactcaacc
     2521 ccgtctcttt cactatcagt cgggcaagat tgcgttcact catcgcaaaa aagccagccc
     2581 attgccccaa tgacccccac tcgacgggcc ctttcgccat catctccacc atcgtgcgga
     2641 ttttaggatg agaagaaacg ggcaggtgca gctgttgttg tggctgctga gggagttcat
     2701 caaacaggac ctgaattaat ctgcgggtca tcggctcagc acgctgagtc gtcgtcctgt
     2761 ttgccagggt taaaattaat tcccggcata agggcgatat tttcaatgtg caacatgttg
     2821 tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg ttagctgtca
     2881 cttgattgct atgcttcacc ccgcccggta tccaaaccgc atattgtggc ggcaccatcc
     2941 acaaagcatt ttccaccgta caggtaattg caccatgtaa tgctagtatc agctgtcctt
     3001 tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata caaaatgcga
     3061 ccgcggcttc atggtgtcgg tcgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctagcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcccactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgctttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc gggcaagttc
     4201 tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt tgctggcagc
     4261 ctgtgggccg ccgctaatgg gtaaaataca cgatgctaac ggtaactggt ccgtaccact
     4321 gatgggtgtt gcaatacttt cactactgat ggcgattttc ggactttgcg ccgggagaga
     4381 caaagaaatt cgctaatatc cggtgctata gtgacgtgac aaatcttgcg tgaaagggag
     4441 aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggctgaac agagcgatgg
     4501 ttatcgcgta ctggtcgacc gcctctggcc gcgcggtatc aaaaaaaccg atttagccct
     4561 tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct ttcacggcga
     4621 agtcgtcgat tttgcaacct ttcgcgagca atatcgcgca gaactggcgc aacacgagca
     4681 agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc tctacgcagc
     4741 aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta gcttgtgatt
     4801 ttagtacggc atccggcggt tatttttcac cagccggatg gtcccgtcgc cacaatgccc
     4861 attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct tgcgctgtct
     4921 gcactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga tttgccatac
     4981 caatctgcgg cggtttaggt gcttctggct gagaaggggt tgaacaacca gccaggacca
     5041 gcaagcaagg cagaacaaaa ctgataattt tcatttattg atctcacata tttatccaag
     5101 cttagagtat cgcggtatcg ttttgttttg cagcactatt tttattacat tcactcaaaa
     5161 catattacgt cttgtttcat ctttgttgat gatgttttat catgcctgca aagattaaat
     5221 aatcagcact tacccgccgt atcctggagt tgttccgtgt cagatcagat tatcgcccgc
     5281 gtctcgcaat cccttgccaa agaacagtca ctggaaagtc tggtccgaca gcttctggag
     5341 atgctggaaa tggtcactga tatggaatca acctacctga ccaaagtgga tgtcgaagcg
     5401 cgcctgcaac atattatgtt tgcccgtaac agccagaaaa tgcacatccc ggagaatttt
     5461 accgtctcgt gggattactc gttatgcaaa cgcgccattg atgaaaactg ctttttcagc
     5521 gatgaagtcc ctgaccgttg gggcgactgt attgcggcac gcaatcttgg catcaccaca
     5581 tttctgagca cgccaattca cttaccggat ggatcattct atggcacgct ttgcgccgcc
     5641 agcagtgaga agcgccagtg gagtgaacgc gcggaacagg ttttacagtt attcgccgga
     5701 ctgattgcac aatatattca aaaagaggca ctggttgaac agctgcacga agccaatgct
     5761 gcgctgattg cgcaatcgta taccgactcg ttaaccgggc taccgaatcg gcgggcgatt
     5821 tttgaaaatc tgacgacgct gttttccctc gcccggcatc ttaaccataa gataatgatc
     5881 gcgtttatcg atctggataa cttcaaatta atcaatgatc gttttggtca taatagtggc
     5941 gatctgtttc tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa tggcgaagtt
     6001 attggtcgtc tcggtggtga tgagtttttg gttgtttcac taaacaacga gaatgcggat
     6061 atttcgtcgc tgcgagaacg tattcaacag caaatacgtg gagaatatca cttaggtgat
     6121 gttgatttgt attatcccgg tgccagtctt ggcatagtag aagtcgatcc tgaaacaacc
     6181 gatgcagaca gtgccctgca tgctgccgat attgcgatgt atcaggagaa aaaacacaaa
     6241 cagaaaacac cttttgtcgc gcatccagcg ctacattcct gaggcgtatt cacatccttt
     6301 tgattggtga taacatgcga atcggtatta tttttccggt tgtaatcttc attacagcgg
     6361 tcgtattttt agcatggttt tttattggcg gctatgctgc cccgggagca taaagatgaa
     6421 aaaaacaacg attattatga tgggtgtggc gattattgtc gtactcggca ctgagctggg
     6481 atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc tgtgtgcagc ctttgtgcaa
     6541 tttaagcgtt aacttttaat cttcctgtag ataaatagca cgacaatcgc accaataacg
     6601 gctgaagcat ttcgtctgca gtctgaaccg caggcaacca tttttcagca agtctgcgct
     6661 gggaatcgct ggctttatcc catttgctcc acagtgcctc acgatcgtaa tcgtgcgctt
     6721 ccagcgtggg gcgggcgatt tcaaaatacc cctggcttgt ttctatttgc tcagatttta
     6781 ataacaactc aacacgtgct gccgctggta gaactgatat atgaaattcc aaagcctttg
     6841 aacccgtacg cttgcgacgt agttctggat gattggctgc aagcttttca agattgcggc
     6901 gaatactcac ctcatgttta ggaaagcatg acattcctac gcattcttgc gctgttaccc
     6961 aatgggttaa catgatgttt tctccatata ccgagaaggc caaatctggg ctggttccat
     7021 atccaaatat ctagcaatga ttgcttcgta tcgtggtata tggcagcgaa atacgttacg
     7081 catagtgtca ggctttaagc ctgcctctct ggataattga gatagtgtct tccccttcat
     7141 acgaacagca gcctttatct gctctgcgtg ccagtcttgg ttttttgctg acattctgcg
     7201 atccttactc tcttaacgtt ttcggtaact gataccggct accgctatcg ggaaaaacga
     7261 tatcgcagaa aaatgcgcat ttcaagagct atgcacattt ttattttaac caatgcaaat
     7321 caaagaattc tgtaaaattc tttatataac agagagattg agcatgacag gtgaaaaaga
     7381 aagcaaaaat gcagagcgca tttttatgcc ccggaaataa tgcgtttcaa ggaacgcctt
     7441 gaagaggcaa tgaatggaga atcaagccgg gcg
//
LOCUS       DAJTXY010000161         7447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTXY010000161 DAJTXY010000000
VERSION     DAJTXY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.161"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..233)
                     /locus_tag="OO566_003735"
     CDS             complement(<1..233)
                     /locus_tag="OO566_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR6606839.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAPIFP"
     gene            753..1448
                     /gene="minC"
                     /locus_tag="OO566_003736"
     CDS             753..1448
                     /gene="minC"
                     /locus_tag="OO566_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6606840.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1472..2284
                     /gene="minD"
                     /locus_tag="OO566_003737"
     CDS             1472..2284
                     /gene="minD"
                     /locus_tag="OO566_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6606841.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2288..2554
                     /gene="minE"
                     /locus_tag="OO566_003738"
     CDS             2288..2554
                     /gene="minE"
                     /locus_tag="OO566_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6606842.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2926..3270)
                     /locus_tag="OO566_003739"
                     /pseudo
     CDS             complement(2926..3270)
                     /locus_tag="OO566_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3305..3424)
                     /locus_tag="OO566_003740"
     CDS             complement(3305..3424)
                     /locus_tag="OO566_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6606843.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3385..3570)
                     /locus_tag="OO566_003741"
     CDS             complement(3385..3570)
                     /locus_tag="OO566_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606844.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3671..3844
                     /locus_tag="OO566_003742"
     CDS             3671..3844
                     /locus_tag="OO566_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606845.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3846..4190
                     /locus_tag="OO566_003743"
     CDS             3846..4190
                     /locus_tag="OO566_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6606846.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4194..4529
                     /locus_tag="OO566_003744"
     CDS             4194..4529
                     /locus_tag="OO566_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6606847.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4586..6907)
                     /locus_tag="OO566_003745"
     CDS             complement(4586..6907)
                     /locus_tag="OO566_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6606848.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPIPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7024..7233)
                     /locus_tag="OO566_003746"
     CDS             complement(7024..7233)
                     /locus_tag="OO566_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606849.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2042 a         1750 c         1654 g         2001 t
ORIGIN      
        1 tctggaaata taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga
       61 tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa
      121 acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag
      181 aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt catagcacat
      241 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
      301 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      361 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      421 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      481 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      541 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      601 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtgacttg
      661 cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg
      721 cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag
      781 gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc
      841 aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac
      901 tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg
      961 caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa
     1021 ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc
     1081 ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata
     1141 ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc
     1201 acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc
     1261 gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc
     1321 tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag
     1381 aattttatgg caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt
     1441 taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg
     1501 taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa
     1561 gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat
     1621 gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct
     1681 aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca
     1741 aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa
     1801 agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt
     1861 aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt
     1921 acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg
     1981 cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag
     2041 aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt
     2101 gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga
     2161 cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga
     2221 acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg
     2281 ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc
     2341 aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta
     2401 tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc
     2461 tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa
     2521 cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt
     2581 caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc
     2641 ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag
     2701 gctgttccca cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta
     2761 gtgtggatat gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg
     2821 atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat
     2881 cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt
     2941 cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat accccacccc
     3001 gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg
     3061 tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc
     3121 gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag
     3181 acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga
     3241 tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc
     3301 aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat
     3361 tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag
     3421 gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata
     3481 atactgtcgt acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt
     3541 aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc
     3601 agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata
     3661 ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat
     3721 tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt
     3781 taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat
     3841 ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc
     3901 cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt
     3961 tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg
     4021 gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg
     4081 ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag
     4141 gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga
     4201 tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg
     4261 actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg
     4321 aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca
     4381 gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag
     4441 aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg
     4501 acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga
     4561 cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg
     4621 tgtcattgta gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg
     4681 gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt
     4741 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     4801 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     4861 aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     4921 cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct
     4981 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt
     5041 aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat
     5101 cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt
     5161 ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac
     5221 tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac
     5281 cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc
     5341 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     5401 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta
     5461 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt
     5521 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg gggataggcg
     5581 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     5641 tagtcagata ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt
     5701 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     5761 tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa
     5821 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     5881 catccaattc agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg
     5941 ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat
     6001 caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa
     6061 gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg
     6121 aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg
     6181 atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt
     6241 catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta
     6301 tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg
     6361 tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt
     6421 ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat
     6481 gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac
     6541 cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga
     6601 tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg
     6661 cagtactttc ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg
     6721 caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa
     6781 aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc
     6841 cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac
     6901 cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc
     6961 ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa
     7021 tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat
     7081 aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag catgtttata
     7141 ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc
     7201 caaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     7261 caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa
     7321 aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag
     7381 tcggcattat catttttcac cttattttca tgacattgat cactttgggg tgactatttt
     7441 gtttaac
//
LOCUS       DAJTXY010000162         7351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTXY010000162 DAJTXY010000000
VERSION     DAJTXY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.162"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            94..1205
                     /gene="yneK"
                     /locus_tag="OO566_003747"
                     /pseudo
     CDS             94..1205
                     /gene="yneK"
                     /locus_tag="OO566_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1355..2545
                     /gene="ydeA"
                     /locus_tag="OO566_003748"
     CDS             1355..2545
                     /gene="ydeA"
                     /locus_tag="OO566_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6606850.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2570..3235)
                     /gene="marC"
                     /locus_tag="OO566_003749"
     CDS             complement(2570..3235)
                     /gene="marC"
                     /locus_tag="OO566_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6606851.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3447..3881
                     /gene="marR"
                     /locus_tag="OO566_003750"
     CDS             3447..3881
                     /gene="marR"
                     /locus_tag="OO566_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6606852.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3901..4306
                     /gene="marA"
                     /locus_tag="OO566_003751"
                     /pseudo
     CDS             3901..4306
                     /gene="marA"
                     /locus_tag="OO566_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
     gene            4338..4556
                     /gene="marB"
                     /locus_tag="OO566_003752"
     CDS             4338..4556
                     /gene="marB"
                     /locus_tag="OO566_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6606853.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4587..5486)
                     /gene="eamA"
                     /locus_tag="OO566_003753"
     CDS             complement(4587..5486)
                     /gene="eamA"
                     /locus_tag="OO566_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6606854.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5750..6868
                     /gene="ydeE"
                     /locus_tag="OO566_003754"
     CDS             5750..6868
                     /gene="ydeE"
                     /locus_tag="OO566_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6606855.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            6995..7090
                     /gene="mgtS"
                     /locus_tag="OO566_003755"
     CDS             6995..7090
                     /gene="mgtS"
                     /locus_tag="OO566_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR6606856.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1918 a         1787 c         1672 g         1974 t
ORIGIN      
        1 acagcgccac gcatcacctg cctttgatag ttcttgcagc tcaaatgccc tatatagtga
       61 tgttccgatg acttatgact atatggggca aatatggtta cgccagtaag catcagcaat
      121 tacatatctc ttcctgatga ttttcccgct cgtaatattg cacctcaagt aaaagaagtt
      181 ttaaaggatt ttattgatgc actcagtacc ataatatgtg atgaagagtg gcgcacgtct
      241 ttaaacacca actcagccac aaaaaagata tttaacaacc ttgataactt atcatatatt
      301 cagaggactt cttttcgggg taacgacaca ctatacaatg aaaaggttca gtttaaactc
      361 acttatcccg tcaagaatgg aagacacaaa gaaaatattg aatttcaggt agtaataaat
      421 ttaagcccta tttagataat tttcgccatg atggagaaat taatattttt tgcgctccca
      481 acccaaagcc tgtcactatg gggcgcgtat ttcagaccgg cgtcgagcgt gtgctctttc
      541 tgtttatgaa tgattttatt gaacaatttc caatgatcaa ccttggcgcc cccatcaaaa
      601 gagcgcatac accacatatt gaacccctgc ctccggatca ccataccgct gcagattact
      661 tacgccagtt tgatttgctt gtcctgaatt ttatctctcg cggtaatttt gtcatactcc
      721 cccgattatg gaataactct gaggttcaca gatggttcgt caataaagat cctaacttga
      781 tcaccgccat tctcgacata acggacagcg aattaaaaga ggatttattg caaagcctga
      841 tggattcatt aggttctaac aaacatgtac aacccgaagt ctgcatctgc ttcttatccc
      901 ttttagcaga acaagaatct cctcattttc aggacttgtt tttatttttc gccaatatgt
      961 tattgcacta tcaccaattt atgaatccca atgaaagtga tctgaatgac gtgttgatgc
     1021 cagcatcatt aagtgatgat aaaattatca aacatatggc gcgcaggacc ctcaaactat
     1081 ttgtaaaaaa tgaaacgccg ccaaaagtta ctcacgaaga cctggtgaaa aacaggcctc
     1141 gctcccctgt cagaccacct atacccgcaa ccgccaaaac gccagacctc cctgaacgtc
     1201 attaaaccgt gatgttaccg actctctgac gcggagcaga atcagcgtca gagaaacgga
     1261 aaacgcgatc cagatcacaa atgcattgta ttcacatcat taaccgtttt aagatcattt
     1321 catcactttt tcgcaactca cccgataatc tgttatgaca acaaacactg tttcccgcaa
     1381 agtggcgtgg ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca acaccaccga
     1441 atttgtccct gttggcctgc tctctgacat tgcgcaaagt tttcacatgc aaaccgctca
     1501 ggtcggcatc atgttgacca tttacgcatg ggtagtagcg ctaatgtcat tgccttttat
     1561 gttaatgacc agtcaggttg aacggcgcaa attactgatc tgcctgtttg tggtgtttat
     1621 tgccagccac gtactgtcgt ttttatcgtg gagctttacc gttctggtga tcagtcgcat
     1681 tggtgtggct tttgcacatg cgattttctg gtcgattacg gcgtctctgg cgatccgtat
     1741 ggctccggcc gggaagcggg cacaggcctt gagtttaatt gccaccggta cagcactggc
     1801 gatggtctta ggtttaccgc tcgggcgcat tgtggggcag tatttcggct ggcgaatgac
     1861 cttcttcgcg attggtattg gggcgcttgt tacccttttg tgcctgatta agttacttcc
     1921 cttactgcct agtgagcatt ccggttcatt gaaaagcctc ccgctattgt tccgccgccc
     1981 ggcattgatg agcatttatt tgttaactgt ggtggttgtc accgcccatt acacggcata
     2041 cagctatatc gagccttttg tgcaaaacat tgcgggattc agcgccaact ttgccacggc
     2101 attactgtta ttactcggtg gtgcgggcat tattggcagc gtgattttcg gtaaactggg
     2161 taatcaatat gcgtctgcgt tggtaagtac ggcgattgcg ctgttgctgg tgtgtctggc
     2221 actgctgcta cctgcggcga acagtgaaat acacctcggg gtgctgagta ttttctgggg
     2281 gatcgcgatg atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc tggcaccaga
     2341 tgctaccgac gtcgcgatgg cgctattctc cggcatattt aatattggaa tcggggcggg
     2401 tgcgttggta ggtaatcagg tgagtttgca ctggtcaatg tcgatgattg gttatgtggg
     2461 cgcggtgcct gcttttgccg cgttaatatg gtcaatcatt atatttcgcc gctggccagt
     2521 gacactcgaa gaacagacgc aatagttgaa aggcccattc gggccttttt taatggtacg
     2581 ttttaatgat ttccaggatg ccgttaataa taaactgcac acccatacat accagcagga
     2641 accccatcag gcgggagatc gcttcaatgc cacccttgcc caccagccgc ataattgcgc
     2701 cggagctgcg taggcttccc cacaaaataa ccgccaccag gaaaaagatc agcggcggcg
     2761 caaccatcag tacccaatca gcgaaggttg aactctgacg cactgtggac gccgagctaa
     2821 taatcatcgc tatggttccc ggaccggcgg tacttggcat tgccagtggt acaaaggcaa
     2881 tattggcact gggttcatct tccagctctt ccgacttgct tttcgcctcc ggtgaatcaa
     2941 tcgctttctg ttgcggaaaa agcatccgaa aaccgataaa cgcgacgatt aagccgcctg
     3001 caattcgcag accgggaatc gaaatgccaa atgtatccat caccagttgc ccagcgtaat
     3061 acgccaccat catgatggca aatacgtaca ccgaggccat caacgactga cgattacgtt
     3121 cggcactgtt catgttgcct gccaggccaa gaaatagcgc aacagttgtt aatgggttag
     3181 ctaacggcag caacaccacc agccccaggc caattgcttt aaacaaatct aacattggtg
     3241 gttgttatcc tgtgtatctg ggttatcagc gaaaagtata aggggtaaac aaggataaag
     3301 tgtcactctt tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat ttagcaaaac
     3361 gtggcatcgg tcaattcatt catttgactt atacttgcct aggcaatatt atcccctgca
     3421 actaattact tgccagggca actaatgtga aaagtaccag cgatctgttc aatgaaatta
     3481 ttccattggg tcgcttaatc catatggtta atcagaagaa agatcgtctg cttaacgagt
     3541 atctgtctcc gctggatatt accgcggcac agtttaaggt gctctgctct atccgctgcg
     3601 cggcgtgtat tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg ggagcactga
     3661 cccgtatgct ggatcgcctg gtctgtaaag gctgggttga aaggttgccg aacccgaatg
     3721 ataagcgcgg cgtactggta aaacttacca ccagcggcgc ggcaatatgc gaacaatgcc
     3781 atcaattagt tggccaggac ctgcatcaag aattaacaaa aaacctgacg gcggacgaag
     3841 ttgcaacact tgagcatttg cttaagaaag tcctgccgta aacaaaaaag aggtatgacg
     3901 atgtccagac gcaatactga cgctattacc attcatagca ttttggactg gatcgaggac
     3961 aacctggaat cgccactgtc actggagaaa gtgtcagagc gttcgggtta ctccaaatga
     4021 gcgttcgggt tactccaaat ggcacctgca acggatgttt aaaaaagaaa ccggtcattc
     4081 attaggccaa tacatccgca gccgtaagat gacggaaatc gcgcaaaagc tgaaggaaag
     4141 taacgagccg atactctatc tggcagaacg atatggcttc gagtcgcaac aaactctgac
     4201 ccgaaccttc aaaaattact ttgatgttcc gccgcataaa taccggatga ccaatatgca
     4261 gggcgaatcg cgctttttac atccattaaa tcattacaac agctagttga taacgtgaca
     4321 acgtcactga ggcaatcatg aaaccacttt catccgcaat agcagctgcg cttattctct
     4381 tttccgcgca gggcgttgcg gaacaaacca cgcagccagt tgttacttct tgtgccaatg
     4441 tcgtggttgt tcccccatcg caggaacaac caccgtttga tttaaatcac atgggtactg
     4501 gcagtgataa gtcggatgcg ctcggcgtgc cctattataa tcaacacgct atgtagtttg
     4561 ttctggcccc gacatctcgg ggcttattaa cttcccacct ttaccgcttt acgccaccgc
     4621 aagccaaata cattgatata cagcccggtc ataatgagca ccgcacctaa aaattgcaga
     4681 cccgttaagc gttcatccaa caatagtgcc gcacttgcca gtccgactac gggcaccagt
     4741 aacgataacg gtgcaacccg ccaggtttca tagcgtccca gtaacgtccc ccagatccca
     4801 taaccaacaa ttgtcgccac aaacgccaga tacatcagcg acaagatggt ggtcatatca
     4861 atagtaacca gactgtgaat catggatgcg gaaccatcga gaatcagcga ggcaacaaag
     4921 aagggaatga ttgggattaa agcgctccag attaccagcg acatcaccgc cggacgcgtt
     4981 gagtgcgaca tgatcttttt attgaagatg ttgccacacg cccaactaaa tgctgcagcc
     5041 agggtcaaca taaagccgag catcgccaca tgctgaccgt tcagactatc ttcgattaac
     5101 accagtacgc caaaaatcgc taaggcgatc cccgccaatt gtttgccatg cagtcgctcc
     5161 ccgaaagtaa acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa
     5221 gccagtccag caggcatacc aaagttaatg gcgcagaaca gaaaggcaaa ctgcgcaaaa
     5281 ctgatggtta atccataccc caagagcaaa ttcagtggta ctttcggtcg tgcaacaaaa
     5341 aagatggccg gaaaagcgac cagcataaag cgcaaaccgg ccagcatcag cggtggcatg
     5401 ttatgaagcc ccactttgat gaccacaaaa tttagccccc atacgaccac taccagtagc
     5461 gccaacaccc catcttttcg cgacattcta ccgcctctga atttcatctt ttgtaagcaa
     5521 tcaacttagc tgaatttact tttctttaac agttgattcg ttagtcgccg gttacgacgg
     5581 cattaatgcg caaataagtc gctatacttc ggatttttgc catgcgtccg atggttaata
     5641 acaacgacga ggcaagaagg gcgctggtag agcgtcgtag ggataagttc atttgtctgt
     5701 ccggcagtgc gtttcgttat gttttatttt agagatgtaa agaaatagca tggctacgct
     5761 gccatttatg accatttact tgagtcgcca gtacagcctg agtgtcgatc taatcggtta
     5821 tgcgatgaca attgcgctca ctattggcgt cgtttttagc ctcggttttg gtatcctggc
     5881 ggataagttc gacaagaaac gctatatgtt actggcaatt accgccttcg ccagcggttt
     5941 tattgccatt cctttagtga ataacgtgac gctggttgtg ctcttttttg ccctcattaa
     6001 ctgcgcctat tctgtttttg ctaccgtgct gaaagcctgg tttgccgaca atctttcgtc
     6061 caccagcaaa acgaaaatct tctcaatcaa ctacaccatg ctaaacattg gctggaccat
     6121 cggtccgccg ctcggcacgc tgttggtgat gcagagcatc aatctgccct tttggctggc
     6181 agctatctgt tccgcgtttc ccatgctttt cattcaaata tgggtaaagc gcagcgagaa
     6241 aatcatcgcc acggaaacag gcagtgtctg gtcgccgaaa gttttattac aagataaagc
     6301 actgttgtgg tttacctgct ctggttttct ggcttctttt gtaagcggcg catttgcttc
     6361 atgcatttca caatatgtga tggtgattgc tgatggggat tttgccgaaa aggtggtcgc
     6421 ggttgttctt ccggtgaatg ctgccatggt ggttacgttg caatattccg tgggccgccg
     6481 acttaaccca gctaacatcc gcgcgctgat gacagcaggc accctctgtt tcgtcatcgg
     6541 actggtcggt tttatttttt ccggcaacaa cctgctattg tggggtatgt cagctgcggt
     6601 atttactgtc ggtgaaatca tttatgcgcc gggcgagtat atgttgattg accatattgc
     6661 gccgccagga atgaaagcca gctatttttc cgcccagtct ttaggctggc ttggtgccgc
     6721 gattaaccca ttagtgagtg gcgtagtgct aaccagcctg ccgccttcct cgctgtttgt
     6781 catcttagcg ttggtgatca ttgctgcgtg ggtgctgatg ttaaaaggga ttcgtgcaag
     6841 accgtggggg cagcccgcgc tttgttgatt taagtcgaac acaataaaaa tttaattcag
     6901 ccttcgttta ggttatctct gctaatatct ttctcattga gatgaaaatt aaggtaagcg
     6961 aggaaacaca ccacaccata aacggaggca aataatgctg ggtaatatga atgtttttat
     7021 ggccgtactg ggaataattt tattttctgg ttttctggcc gcgtatttca gccacaaatg
     7081 ggatgactaa tgaacggaga taatccctca cctaaccggc cccttgttac agttgtgtac
     7141 aaggggcctg atttttatga cggcgaaaaa aaaccgccag taaaccggcg gtgaatgctt
     7201 gcatggatag atttgtgttt tgcttttacg ctaacaggca ttttcctgca ctgataacgg
     7261 atcgttgaca cagtagcatc agttttctca atgaatgagg tgtactggca atagcggaca
     7321 ctaccatttg ttcttttttt aagcagccat c
//
LOCUS       DAJTXY010000163         7246 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTXY010000163 DAJTXY010000000
VERSION     DAJTXY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.163"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..891
                     /locus_tag="OO566_003756"
     CDS             <1..891
                     /locus_tag="OO566_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6606857.1"
                     /translation="EICDDVTVFRDGQFIAEREVASLTEDSLIEMMVGRKLEDQYPHL
                     DKAPGDIRLKVDNLCGPGVNDVSFTLRKGEILGVSGLMGAGRTELMKVLYGALPRTSG
                     YVTLDGHEVVTRSPQDDLANGIVYISEDRKRDGLVLGMSVKENMSLTALRYFSRAGGS
                     LKHADEQQAVSDFIRLFNVKTPSMEQAIGLLSGGNQQKVAIARGLMTRPKVLILDEPT
                     RGVDVGAKKEIYQLINQFKADGLSIILVSSEMPEVLGMSDRIIVMHEGHLCGEFTREQ
                     ATQEVLMAAAVGKLNRVNQE"
     gene            896..1855
                     /gene="rbsC"
                     /locus_tag="OO566_003757"
     CDS             896..1855
                     /gene="rbsC"
                     /locus_tag="OO566_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6606858.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIVGT
                     LIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            1880..2757
                     /gene="rbsB"
                     /locus_tag="OO566_003758"
                     /pseudo
     CDS             1880..2757
                     /gene="rbsB"
                     /locus_tag="OO566_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            2883..3812
                     /gene="rbsK"
                     /locus_tag="OO566_003759"
     CDS             2883..3812
                     /gene="rbsK"
                     /locus_tag="OO566_003759"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6606859.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQLLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            3816..4808
                     /gene="rbsR"
                     /locus_tag="OO566_003760"
     CDS             3816..4808
                     /gene="rbsR"
                     /locus_tag="OO566_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6606860.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(4774..6201)
                     /gene="mdtD"
                     /locus_tag="OO566_003761"
     CDS             complement(4774..6201)
                     /gene="mdtD"
                     /locus_tag="OO566_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6606861.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVTRLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6224..6916)
                     /locus_tag="OO566_003762"
     CDS             complement(6224..6916)
                     /locus_tag="OO566_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6606862.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1836 a         1751 c         1917 g         1742 t
ORIGIN      
        1 gagatttgcg atgacgttac cgtttttcgt gatgggcaat ttattgctga gcgcgaagtg
       61 gcatcactga ccgaagattc gctgattgag atgatggtgg gccgcaagct ggaagatcaa
      121 tatccacacc tggacaaagc gccaggagat atccgcctga aagtcgataa tctctgcgga
      181 cctggcgtta acgatgtctc ttttacttta cgcaaaggcg aaattcttgg cgtctctggt
      241 ttgatgggcg caggtcgtac cgaactgatg aaagtgctct acggcgcact gccgcgcacc
      301 agcggttacg tcaccctgga tgggcatgaa gtcgttaccc gttcaccgca ggatgacctg
      361 gcaaacggca ttgtgtatat ctccgaagac cgtaaacgtg acggtttagt gctgggcatg
      421 tcagtaaaag agaacatgtc gctgacagcg ctgcgctact tcagccgcgc tggcggcagt
      481 ttgaagcatg ccgatgaaca gcaggctgtg agtgatttca ttcgtctgtt taatgtgaaa
      541 acgccgtcga tggaacaggc aattggtctg ctttccggtg gcaatcagca aaaagtggcg
      601 attgcccgcg gtctgatgac acgccccaaa gtattgatcc ttgatgagcc tacccgtggc
      661 gtagatgtcg gcgcgaaaaa agagatctat caactgatta accagttcaa agccgatggc
      721 ttgagtatca ttctggtgtc atcggagatg ccagaagtat taggcatgag cgatcgcatc
      781 atcgtcatgc atgaagggca tctctgcggg gaatttactc gtgagcaggc cacccaggaa
      841 gtgttaatgg ctgccgctgt gggcaagctt aatcgcgtga atcaggagta aaaaaatgac
      901 aacccagact gtctctggtc gccgttattt cacgaaagcg tggctgatgg agcagaaatc
      961 gcttatcgct ctgctggtgc tgatcgcgat tgtctcgacg ttaagcccga actttttcac
     1021 catcaataac ttattcaata ttctccagca aacttcggtg aacgccatta tggcggtcgg
     1081 gatgacgctg gtgatcctga cgtcgggcat agacttgtcg gtaggttctc tgttggcgct
     1141 aaccggcgca gttgctgcat ctatcgtcgg cattgaagtc aatgcgctgg tggctgtcgc
     1201 tgctgctctc acgttaggtg ccgcaattgg tgcggtaacc ggggtgattg tagcgaaagg
     1261 tcgcgtccag gcgtttatcg ctacgctggt tatgatgctt ttactgcgcg gcgtgaccat
     1321 ggtttatacc aacggtagcc cagtgaatac cggctttact gagaacgccg atctgtttgg
     1381 ctggtttggt attggtcgtc cgctgggcgt accgacgcca gtctggatca tggggattgt
     1441 cttcctcgcg gcctggtaca tgctgcatca cacgcgtctg gggcgttaca tctacgcgct
     1501 gggcggcaac gaagcggcaa cgcgtctttc tggtatcaac gtcaataaaa tcatcgtcta
     1561 ttctctttgt ggtctgctgg catcgctggc cgggatcatt gaagtggcgc gtctctcctc
     1621 tgcacaaccc acggcgggga ctggctatga gctggatgct attgctgcgg tggttctggg
     1681 cggtacgagt ctggcgggcg gaaaaggtcg cattgttggg acgttgatcg gcgcattaat
     1741 tcttggcttc cttaataatg gattgaattt gttaggtgtt tcctcctatt accagatgat
     1801 cgtcaaagcg gtggtgattt tgctggcggt gctggtagac aacaaaaagc agtaataacg
     1861 actacaggac atcttgaata tgaacatgaa aaaactggct accctggttt ccgctgttgc
     1921 gctaagcgcc accgtcagtg cgaatgcgat ggcaaaagac accatcgcgc tggtggtctc
     1981 cacgcttaac aacccgttct ttgtatcgct gaaagatggc gcgcagaaag aggcggataa
     2041 acttggctat aacctggtgg tgctggactc ccagaacaac ccggcgaaag agctggcgaa
     2101 cgtgcaggac ttaaccgttc gcggcaccaa aattctgctg attaacccga ccgactccga
     2161 cgcagtgggt aatgctgtga agatggctaa ccaggcgaac attccggtta tcactcttga
     2221 ccgccaggca acgaaaggtg aagtggtgag ccacattgca tctgataacg tactgggcgg
     2281 caaaatcgcc ggtgattaca tcgcgaagaa agcgggtgaa ggtgccaaag ttatcgagct
     2341 gcaaggcatt gccggtacat ccgcagcccg tgaacgtggc gaaggcttcc agcaggccgt
     2401 tgctgctcac aagtttaatg ttcttgccag ccagcagatt ttgatcgcac caaaggtttg
     2461 aacgtaatgc agaacctgtt gaccgctcat ccggatgttc aggctgtatt cgcgcagaat
     2521 gatgaaatgg cgctgggcgc gctgcaaact gccggtaaat cggatgtgat ggtcgtcgga
     2581 tttgacggta caccggatag cgaaaaagcg gtgaatgatg gcaaactagc agcgactatc
     2641 gctcagctac ccgatcagat tggcgcgaaa ggcgttgaaa ccgcagataa agtgctgaaa
     2701 ggcgagaaag ttcaggctaa gtatccggtt gatctgaaac tggttgttaa gcagtagttt
     2761 taatcaggtt gtatgacctg atggtgacat aaatacgtca tcgacagatg aacgtgtaat
     2821 ataaagaaaa gcagggcacg cgccacccta acacggtggc gcattttatg gacatcccga
     2881 atatgcaaaa cgcaggcagc ctcgttgttc ttggcagcat taatgctgac cacattctta
     2941 atcttcaatc ttttcctact ccaggcgaaa ccgtaaccgg taaccactat caggttgcat
     3001 ttggcggcaa aggcgcgaat caggctgtgg ctgctgggcg tagcggtgcg aatatcgcgt
     3061 ttattgcctg tactggcgat gacagcattg gcgagagcgt tcgccagctg ctcgccactg
     3121 ataacatcga tattactccg gtcagcgtga tcaaaggcga atcaacaggt gtggcgctga
     3181 tttttgttaa tggcgaaggt gagaatgtca tcggtattca tgccggcgct aatgctgctc
     3241 tttccccatc gctggtggaa gcgcaacgtg agcgtattgc caacgcgtca gcgttattaa
     3301 tgcagctgga atcaccactc gaaagtgtga tggcagcggc gaaaatcgcc catcaaaata
     3361 agactatcgt tgcgcttaac ccggctccgg ctcgcgaact tcctgacgaa ctgctggcgc
     3421 tggtggacat tattacgcca aacgaaacgg aagcagaaaa gctcaccggt attcgtgttg
     3481 aaaatgatga agatgcagcg aaggcggcgc aggtactgca tgaaaagggt atccgtactg
     3541 tactgattac tttaggaagt cgtggtgtat gggctagcgt gaatggtgaa ggtcagcgcg
     3601 ttcctggatt ccgggtgcag gctgtcgata ccattgctgc cggagatacc tttaacggcg
     3661 cgttaatcac ggcgttgctg gaagaaaaac cattgccaga ggcgattcgg tttgcccatg
     3721 ctgccgctgc gattgccgta acacgtaaag gcgcacaacc ttccgtaccg tggcgtgaag
     3781 agatcgacgc atttttagac aggcagaggt gacgcttggc tacaatgaaa gatgttgccc
     3841 gtttggcggg cgtttctacc tcaacagttt ctcacgttat caataaagat cgcttcgtca
     3901 gtgaagcgat taccgccaaa gttgaagcgg cgattaaaga acttaattac gcgccatcag
     3961 ctctggcgcg tagcctcaaa ctcaatcaaa cacataccat tggcatgttg atcactgcca
     4021 gtaccaatcc tttctattca gaactggtgc gtggcgttga acgcagctgc ttcgaacgcg
     4081 gttatagtct cgtcctttgc aataccgaag gcgatgaaca gcggatgaat cgcaatctgg
     4141 aaacgctgat gcaaaaacgc gttgatggct tactgttact gtgtaccgaa acgcatcagc
     4201 cttcgcgtga aatcatgcaa cgttatccga cagtgcctac cgtgatgatg gactgggctc
     4261 cgttcgatgg cgacagcgat cttattcagg ataactcgtt gctgggcgga gacttagcaa
     4321 cgcaatatct gatcgataaa ggtcataccc gtatcgcctg tattaccggt ccgctggata
     4381 aaactccggc gcgcctgcgg ttggaaggtt atcgggcggc gatgaaacgt gcgggtctca
     4441 acattcctga tggctatgaa gtcactggtg attttgaatt taacggcggg tttgacgcca
     4501 tgcgccaact gttatcacat ccgctgcgtc ctcaggccgt ctttaccgga aatgacgcta
     4561 tggctgttgg cgtttaccag gcgttatatc aggcagagtt acaggttccg caggatatcg
     4621 cggtgattgg ctatgacgat atcgaactgg caagctttat gacgccacca ttaaccacta
     4681 tccaccaacc gaaagatgaa ctgggggagc tggcgattga tgtactcatc catcggataa
     4741 cccagccgac ccttcagcaa caacgattac aacttactcc gattctgatg gaacgcggtt
     4801 cggcatagat ttacgctgtc ttttgatcaa attattacca tcggttgttt tcagaagcat
     4861 gaacattgct gctgaagcaa cagtaataat gcccatcgtg ataaacgtat agtggaattg
     4921 ttcgacagtc gttgtgcctt ccatcccttc ataaatgcga aggacggccg cacttacagc
     4981 aacgcctaaa ctaatcgaca gttgctgcgt gaccgccaga acattgttac cgctgctggc
     5041 gttgtcatcg gtcagatcgg caagtgtgat ggtattcatc gcggtaaact gcgtcgacat
     5101 agccatccct aatataaaca acggcaagat cagcatccat atcgccattg ccggtgattg
     5161 caaagagaac tgagcgatca ttagcccaat aatcaccgtg atccccacta atgtatggcg
     5221 atagcccaga cgacgtaaga cttgggtaac catcgatttt gcaataatgg aacctaacgc
     5281 tgtcggtgcc atcatacagc cggcaataaa cgcctgataa ccaaatccta cctgtaacat
     5341 caatggcata aggaacggta cacagccggt ccccagacgg gttgcaatat tgcctacgat
     5401 accgatcgag aaagtgcggg ttttaaataa atctaatgaa attaatgggt ttggcgtacg
     5461 tcgcgcatgg agaatataga gaagcagtaa cccgatgctg gtgacaatta ccgtcaaggc
     5521 aatccagctg gcgacaatct tttccccgaa tagctctatt ccgcttgaaa agagaacaag
     5581 gctgaggcca aacagcaaaa agccagtgat atcgaatctg cgtcgtgcgg tggtgaaatt
     5641 gggcatatgt ttgcgcgcgt aaagaaggcc cgcaatacct atggggatat taattaaaaa
     5701 tatccagtgc caggttgccc aggtgaccag cacgccgcca agaacggggc ctaaaattgg
     5761 ccccaccaga cccggcatgg cgacaaaatt caataccgga agaagttcat tacgaggata
     5821 agcacgcagt aaggccagcc gagtaacagg catcatcatt gcgtcgccta tcccttgaat
     5881 aacccggaag acaaccagct gtggtagcga attagaaagt gcgcaggcca gagaacccaa
     5941 cgtgaacaga ctcacggcaa gggtaaaaat gcgacgcgta ccgaagcgat cggctagcca
     6001 tccgcttacc ggaataagca tcgccaccgt cagcgtataa ctgatgatgg ctgattgcat
     6061 cgcgagagga gaacgattaa ggctatgagc gattgcgggt aaggcggtat tcagaatagt
     6121 ggcatcaagt gcctgcatga aaaaggccat cgccgcgatc cacggcaaac ccgacatact
     6181 acgcttcttt ttatcgctca ttcaatgtcc tgttatcggg ttatcactta tcaggtgagc
     6241 gtagcagcgc ctgacaagct ttaaatgccg cgtcgccatc gctttggata atcgcatcga
     6301 caatcgcctg gtgcagatcc agctttatca ctgtgtcgct ggtaattgac gtgaagtaag
     6361 tgtgataaac cgaatggaat agcgaggcga atgaggtcaa aaacggattg gcgctcattt
     6421 catagatatg ctcatgccag gctatatcga cttcgatcca gcgttcacgg cgaaagttct
     6481 cttttaatgc cgccatttcg gccattaacg tattgagatg cgccttctgt tccgcggtgc
     6541 caaccgttgc tgccagtagg caggcttgcg gctccagaca gatacgcata accagaaagt
     6601 gatcgatgac ctgatgaaag ttctcttctg tcatccacca agtaagcaat tcctgatcaa
     6661 gaaaattcca gttcgattgt ggcatgaccc gagtaccaat tcgcggtcgc ggtaaaacca
     6721 tcccttttgc cgttaacgtt ttgaccgctt cgcgtaccgc tgtacgactc actccaaatt
     6781 gctcgcccag ctcaatctca ccgggcaaaa tggtgccggg ttcatattca ccttttaaga
     6841 tccgttgcgc cagcttctca gccagaacat acgaaaggtt tttctgtgca gctaactgtt
     6901 gtgcgcttaa aggcattact tatcttcctt tttcttttta ttcctcctta gtatgccacc
     6961 aggaagtgtg attacggttg caaaaacggc aaattgattg ttttatggca cattaacggg
     7021 gcttttgctg aaaaaatgcg cggtcagaaa attattttaa atttcctctt gtcaggccgg
     7081 aataactccc tataatgcgc caccactgac acggaacaac ggcaaacacg ccgccgggtc
     7141 agcgggattc tcctgagaat tccgtcagag aaagcaaaaa taaatgcttg actctgtagc
     7201 gggaaagcgt attatgcaca cctcgcgccg ctgagaaaaa gcgaag
//
LOCUS       DAJTXY010000164         6979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTXY010000164 DAJTXY010000000
VERSION     DAJTXY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.164"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..210)
                     /locus_tag="OO566_003763"
     CDS             complement(<1..210)
                     /locus_tag="OO566_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR6606863.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINAS"
     gene            complement(207..2591)
                     /locus_tag="OO566_003764"
     CDS             complement(207..2591)
                     /locus_tag="OO566_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCR6606864.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            complement(2809..3681)
                     /locus_tag="OO566_003765"
     CDS             complement(2809..3681)
                     /locus_tag="OO566_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR6606865.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHWGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            4012..4194
                     /locus_tag="OO566_003766"
     CDS             4012..4194
                     /locus_tag="OO566_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606866.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNSEDN
                     DEAASDYALKINESSD"
     gene            complement(4494..4919)
                     /locus_tag="OO566_003767"
     CDS             complement(4494..4919)
                     /locus_tag="OO566_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR6606867.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPGEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMMLENPSPELLTVLIRELSGRGR"
     gene            complement(6037..6549)
                     /gene="clbS"
                     /locus_tag="OO566_003768"
     CDS             complement(6037..6549)
                     /gene="clbS"
                     /locus_tag="OO566_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin self-protection protein ClbS"
                     /protein_id="HCR6606868.1"
                     /translation="MAVPSSKEELIKAINSNFSLLNKKLESITPQLAFEPLLEGHAKG
                     TTISVANLVSYLIGWGELVLHWHDQEAKGKTIIFPEEGFKWNELGRLAQKFYRDYEDI
                     TEYEVLLARLKENKQQLVALIERFSNDKLYGKPWYNKWTRGRMIQFNTASPYKNASGR
                     LNKLQKCLAE"
     gene            complement(6584..6865)
                     /locus_tag="OO566_003769"
     CDS             complement(6584..6865)
                     /locus_tag="OO566_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCR6606869.1"
                     /translation="MTIFTPIIKNDYRLYEQYVFQAKARTLTCPIVLFHGDADNLVMQ
                     DELLAWEKFTTRKTRTIIFPAADHFFVDKHFEQVVGYVNQTIESLEIVG"
BASE COUNT         1742 a         1752 c         1593 g         1892 t
ORIGIN      
        1 cgaagcattg atggcattct gccgtgcagt accttcgccg ctcagcccct cttttaaccg
       61 ccggaagaac gtgcgctctc cggccgtacg gatatgctcc tgtgcaacat caataccgtt
      121 tgcgaaatca accacaaatt tttcaggaag acaggtgagt acctgacata cccggttatc
      181 agcaatttgt tgaataaatg gtgaagtcac agcattgtct ccagttcttc ccttaacgcc
      241 ttactgtcag taatgagctc aggaaccctt tcatttaacg cctgaagacg cccggtcagc
      301 cgttcctgcg cctgctgatc gcgtttatga tcttcagagc tctgaatcag cgtgttacgc
      361 agctgttcaa ctttctccct gaatgtacag aaaaaggcat cgatttcctg gcggacgggc
      421 tggttaatat cctgctcaat ggatgcctgc agctccccga aatatgcccg ggtcagtgac
      481 atcacttcct ctctgacctt gttgatattg atcacgctgc ggctcacatc cacactgtac
      541 tctttccagc cccagtcact ggtgtttgcc catcgacaaa gcgtacccca gacaccatcc
      601 tgtcgacagc gacgggttac tgtctccgtt ctttcctcca taagcgcgct cagatttgtt
      661 tttactgcga gccgggtctg taactggctg actgccggaa aactgatttt tacagtgaaa
      721 ccttcatcct ccagacgggc attgatgcgt ccggcaatat tttctgtggc gtacagggcc
      781 gtaccacgaa aacctgtttc aatacccctg acgatttttt ccagttcagg acggaactgt
      841 gcttcagcct cacggtgcag gctggtcacg gtcgattcga tttgcgtcat cagttcaagg
      901 gcttccctgc gatcactgaa ttttatttcg gggctgtccg gatcaaagtc ccgcatcctg
      961 tcatgctgat ttccggttcc gaaaatatcg tgaaagaaag cgaatgcgtt tctctcccgg
     1021 ggctgcgctg aattttcttc ctccagcatt tgctgttctt tgcgtttacc gcttctgaaa
     1081 tagtcattca gctcctcttc cagtgaggtg cagacccccg tcagtaattc ccgggttctg
     1141 gcatcgatct cctccatggc cattctgaca tcagcggtca cctgctcctg acagtccgcg
     1201 atttcccgga tatccgcaag caggctggtg atatgcgact gcagtgactg aatgctttgc
     1261 tgtagcccct gatggcgtaa tgacagatat tcgctgacat tttccgcatt ctgcatcagt
     1321 tttgcggcgg cagaatcaac cgccagtgcc gccgctctgg aatgggagct ctgtatgact
     1381 tccgttatca gttgatcgat aagtgagcct tcccacaggt ctgtcgcccc ttcggttgcc
     1441 atttcactgt ctttacagac gtattctttt ttcatgcgac cgaaggcttc cctgacaaaa
     1501 tcatcgaccc aggcttcatc aacaggaagg gttctgtttt tccgtaactc atgaagcgca
     1561 cggttagcca ggtacgcctg acgtgatgag cctggataaa cgcgggaggc gggcagcaca
     1621 tcgctgttca gcattgccgg aactttctgg cgtacggcat ctgccccatc gccattgcgg
     1681 tctttctcat caaatttatt gaccagcaca aacaggcggc cggctgatac gtcagcaatg
     1741 gcattaagct ctttacggac gtcttcatcc gcttttgagt tcatctgggt gtaatccatc
     1801 accgccagca ccgcagaggc tttctgcagt tgatcccgca tcatcacttc catctgcggt
     1861 tgtccggcct cattagggcc gggggtatcc agcagtgtga gtgttccctg gcatgcatcc
     1921 atccccacca gatggctgaa ttccacgtcg atcaccggca gtttctgcac ttctgcgtat
     1981 tcatcaaaag gaaactcact ccccattgcc gcagcaaggc gaaccagatc gtttaacgat
     2041 gccagtccgg taaagatttc gtcttccccg tggtattcat ttttgagcca gccatcattt
     2101 gccagaatat ccagcagttc gcaggtatcc ccggtttgct gcagggtctg tgcgacttgc
     2161 tgtcctgccg gggtgtcgag tttttgttgc agtgtgctta ataaattgcg gacgggctga
     2221 atatgttcca gatggaggac cggctcagtt tttccgggaa cgtggcggat gagcgtcggt
     2281 acagaggtca tggggcggtt acggttaggc agaatttcct gccccacaat ggcgttgatg
     2341 gtagttgatt tacctgcttt catcgtgccg acaacagcca gcaccatctc cctgcgggcg
     2401 gttttgattt gttcgccctc cagctcctca attcttttca gtgctttctg tttatcaaaa
     2461 agcagtctgt tttcgttctt actgtcagat aacacatccg gttcatcaac catttgccgg
     2521 agcagattaa tgttcagttg caaaagtcgg tcggcttcat cacaaagcag ggcgatgttc
     2581 ttttcgtgca ttatttaacc ttcctgagac gcattttcat gacaaccacg gaggaggtca
     2641 tttaccacaa acgatcgttt atagcgatcg atatgatagt aacgatcgat gcattctatt
     2701 aacttgagaa acacaacgca aggggacaac ccgggtatgg ctgtgtttgc attgtgtttt
     2761 catggggtta aaaatctgta acaagagtct ggtgcatcac caccgggttc agaagaaaat
     2821 ccagttccat accgcgcggg caacagagac cacggtgtca cgaacagcgc gcagaacctt
     2881 acgggcaaca gaggcaacac agacgctctc cgccgtgtca aatatccggt ccaccgcacc
     2941 ggtaaactgt tcacgggcct gaccccggac agattccgtg cgcagcccgt cctgcaggcg
     3001 ggtcatcagg ggactggcgg catggtcggg aagcgctgtc atgagcgcac tgaccagcgt
     3061 atccagttcc cagccagtgc gggccgatac ggccacaacc ggatgtacgg gccggaacag
     3121 acggaatacc gcctccgttt tttcgcgaat attctgtgcc tgtgcaggag aaggctgaat
     3181 accggccata tcccattcat ggcagggctc cgttttgtcg gcctgcgtca ccacaaacag
     3241 tacccgctga tgtccccagt gcaggatgtg tcgccagaaa tactcatcca cagacagggc
     3301 acggtcatcg gctttaatca gccacagtac caggtccagt tcaggcagaa tgtcacggta
     3361 cagggcctca tactctgcat ccctgtcccg gctctcgccc accccgggca ggtcagtgat
     3421 aaccatgctg tgaccatggc cactcagacg gaagcgccgt acttcccggg tgccggcgtg
     3481 aacatcactg accggggtga cctccccctg aaacagtgca ttacagagtg aggatttacc
     3541 ggccccgctt ttacccataa tgccaatcac gggttcgtga ctggtgagtt tgcgcagatg
     3601 ttccaggatg tgacgggaaa gtgagtaagg cagggaggag agcggttttt caattgcctc
     3661 aatggcatca gacggattca tacatgtatt cccggtgaaa aaagacaaaa ccccgcgcac
     3721 cggaaaggtg gcgggggact cacaacgata atatctgtct gaaattattt aggatacagg
     3781 tatcttcaga taatcatacc tccagaattt tgttcttaat agttgagtag tggtatttgg
     3841 tttccctgag agaatataaa ggtgaatcat gctgcactga gtttgattct atttgtactg
     3901 agacaagtat cttcatgatt ataatcaaaa gttgggtggg ttggtctggc gctcatcatt
     3961 ttttcgaata aactcactaa ttattgtaac caccccaaag gagatgattt catgtataat
     4021 tgtggtgaat catattcaga atacgacata tcattatctg acgatgaaaa ctatgacgat
     4081 gactattatc gtgaacgccc gtgtccgggg gaagttttcg gctatgataa tagtgaggat
     4141 aatgatgagg cggcatctga ttatgcacta aaaataaatg aaagctctga ttaatacggg
     4201 ggaagctgat agtaagccaa tcaataacag atattaatta atgaatgaat gaatgaatga
     4261 ataaatataa tcaggcgctc aatattttat ttaatcttgg cgcctgatta aaatccgagg
     4321 taacatataa taattctcat caaaatagtg gatttgcccc tatatttctg agtatctgtt
     4381 atcacttaat ccattacaga tcctccttca gcatatgtcg tatccgttga aagatttaca
     4441 catatcggta acaccggcaa cgagccagat acgggtacct gatgggagtg aaatcatctt
     4501 cccctcccgc tcagttcgcg gatcaacacc gtgagcagct ctggcgatgg attttccagc
     4561 atcatgttac cgtgacggaa ctccaccttg caggaactgg cactgactct ggtctgagtg
     4621 gaagtggata aagacggcgc aatggccgcc acaggttctt tctgctcacc cggcgttatt
     4681 tctacaggta ataattcaac gccagtgtca gaagaggtcg ttaccggaag acgccgcgaa
     4741 acacgccctt cgttctgcca gagcctgagc catttgaaaa taacattatc attgacgcca
     4801 ttttcacgtg caatctgtgc aacacaagct ccaggttgtg atgccagttc caccatacga
     4861 agtttgaatt cattcgaata gtttttacga ggttcttttc gccagtcctg taattccata
     4921 cttagatgtc cgtctatatc agatgggcgt ctaagttacc aattctcgtc tgatggctac
     4981 atacggcggt cagtttacgc ttactacaat gatgataatg cgattatata aaggtcaaac
     5041 catatggttg ttagcggatt tcaacaacgg tgctgtactc gctgagcggc acatgaagtt
     5101 tgaataatgt ggaaacaaca aatggctaca ttaagtcatt ggacatatac agagatggtt
     5161 ggcaaatcga gtacacgata tgttgcctga aaagtcgacc gaacagaact ataaatatca
     5221 agtcggcata acacatctaa atttcataag ggaagatgtg ccaccgggga cgtttttttc
     5281 accacgggga cttcgggtat aacgatgcaa tccgaagaaa gaaatccaat gttggcgata
     5341 ttcatcatcg ctttattcgt ccactcatgc gaatggcaat aaagcgactc accaaaacag
     5401 tttctttagc ttccctctac caaaattccc tatgatcgca tgttagaact ctgcgtgctg
     5461 aacttcgggc catatggacg gtatcattca tgcacttgct cctttgaaat tgctggatat
     5521 cattcgtctt tatctgcacc ggccggcaca aatagctgta ggggaacccg gcgcaggcaa
     5581 aaagagcgac tttaacgcgt tttgcaggac tccccgttgc cttgcaggga gacactgtat
     5641 catcaaacac catcttaatc ccagtacatt accctccatc ctggctcgta ccaaatcaga
     5701 ggaaaggcct cgcggcaata gattttcccg gcgaagctcg gagtgaaata cagtgcagca
     5761 atatccgaca tcagaatttg atcctcctgc gtcaggatac gaaaaaacat cccgatttga
     5821 gcacccttgt cgaaaaaaaa tcaccttatt atgaagattg gttcttaata cacatgcgtt
     5881 cgcatgtgct tatgttaagt agacttattg tcttacacat tttatccgta tcattaacca
     5941 gaatatgttc cggagcagcg ggtgatagtg ccgccagtcc acactcatcg ctgcccggaa
     6001 aaatcatcag tggggaggca aacggtaagc accccgctat tctgcaagac atttctgcag
     6061 tttatttaac ctccccgaag catttttata aggcgaggcg gtattaaatt gaatcatacg
     6121 acctcgggtc catttattat accaaggttt accgtaaagc ttgtcgttac tgaatcgttc
     6181 aatcaaagcc acgagttgct gcttattttc ctttaaccgt gccaataaaa cttcgtactc
     6241 tgtaatatcc tcatagtcac ggtagaattt ctgtgctaaa cgccccaatt cattccattt
     6301 aaatccttcc tcaggaaaaa taatagtttt tccttttgcc tcttggtcat gccagtgtaa
     6361 caccagctct ccccagccaa tcagatagga aaccagattc gctacgctaa tcgtagtccc
     6421 cttcgcgtgc ccttccaata gaggttcaaa ggcgagttgg ggcgtaatag attctagctt
     6481 cttatttaat aaagaaaaat tactattaat agctttaatt aactcttctt ttgatgatgg
     6541 aacagccata tctattgctc cttgtatagt tacacaacta tttctaccct actatttcga
     6601 gtgattcaat cgtctggttc acataaccta ccacctgttc aaaatgctta tcgacaaaaa
     6661 aatgatcggc agcaggaaat ataatagtcc gcgtctttcg tgtggtgaat ttttcccatg
     6721 caagtaattc atcctgcatt accagattgt cagcatcgcc atgaaatagc acgatcggac
     6781 aggttaatgt gcgcgccttg gcctgaaata catactgctc atagagccga taatcgtttt
     6841 taatgatggg ggtgaaaatt gtcattaatg aaccgccccg ggtttcctgg agagtgtttt
     6901 atctgtgaac tcaggctgcc agatcatcgt ttccgatgga agcataataa gctttttctg
     6961 cttctgccgg aggagtatg
//
LOCUS       DAJTXY010000165         6908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTXY010000165 DAJTXY010000000
VERSION     DAJTXY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.165"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..383
                     /locus_tag="OO566_003770"
     CDS             <1..383
                     /locus_tag="OO566_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6606870.1"
                     /translation="LIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPL
                     NGYSICAFPDAADKQTPDIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLT
                     GDSPFAANALGKLAAQEMLAAYAS"
     gene            complement(491..1849)
                     /gene="hprS"
                     /locus_tag="OO566_003771"
     CDS             complement(491..1849)
                     /gene="hprS"
                     /locus_tag="OO566_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6606871.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(1849..2520)
                     /gene="hprR"
                     /locus_tag="OO566_003772"
     CDS             complement(1849..2520)
                     /gene="hprR"
                     /locus_tag="OO566_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6606872.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2653..3066
                     /gene="hiuH"
                     /locus_tag="OO566_003773"
     CDS             2653..3066
                     /gene="hiuH"
                     /locus_tag="OO566_003773"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6606873.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3175..4179
                     /gene="msrP"
                     /locus_tag="OO566_003774"
                     /pseudo
     CDS             3175..4179
                     /gene="msrP"
                     /locus_tag="OO566_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4180..4814
                     /gene="msrQ"
                     /locus_tag="OO566_003775"
                     /pseudo
     CDS             4180..4814
                     /gene="msrQ"
                     /locus_tag="OO566_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5072..5722
                     /gene="zinT"
                     /locus_tag="OO566_003776"
     CDS             5072..5722
                     /gene="zinT"
                     /locus_tag="OO566_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6606874.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMISIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(6800..>6908)
                     /locus_tag="OO566_003777"
     CDS             complement(6800..>6908)
                     /locus_tag="OO566_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6606875.1"
                     /translation="ENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
BASE COUNT         1942 a         1571 c         1439 g         1956 t
ORIGIN      
        1 cacttattgg tttaccagaa agccaggacg tggctgccgc tttacagtgg gcaatcaaaa
       61 atgaccgttt tgttatctcc ctttgccacg gtccggcggc ttttctggca cttcgccaca
      121 gcgataaccc actgaatggt tattctattt gcgcattccc agacgccgca gacaaacaaa
      181 cgccagatat tggctatatg ccgggtcatc tcacctggta cttcggcgaa gagttgaaga
      241 aaatgggcat gaacatcatt aatgacgaca tcaccgggcg agtacataag gaccgtaaac
      301 gtctcaccgg cgacagtcct tttgcagcga atgcgttggg taaactggcg gcgcaggaaa
      361 tgctggcagc ttacgcgagt taatccctgt tacattgtga tgtaagcgca taatcaatcg
      421 cagccacgtt aatactcacg acgtggctgc gttttttcac ggttaactta tggcgtactg
      481 aagcccgatg ttaatttctt tgcggtaacg taattcggaa cacgttatgc ttactgagat
      541 agtgatacgt agcacttcct ccatgtaatt cggcaatcgc tttgactaaa gaaaggccaa
      601 gtccctgacc tacggaatga cgcgaattat ctccccgcca aaatctacgg aagagttttt
      661 caggctcatt aattttcgtt ccaggactgg cgatatcaat attaagagag ccgttggcat
      721 caagaaaact ggttatatga atacgcgatt tttctggtga atatcgaatg gcgttaacaa
      781 taagattcga taacattcgt tgcagtaaaa ttttatccgc aaagatttgt tgattgcact
      841 tgaccttaaa gcaaatctct ttctcgtctg aaagagattc aagatagtcc aaaagatttt
      901 caacttcctt attgagagaa agcgaatcca gtttcaccaa aacattgttt ttgtctgccc
      961 tggcaagaaa cagtatattc tctgttaacc gcgaaatatt ttccagctct tcaatgtttc
     1021 cggcaattgt tttttgatat tcagcgatac ttctgatttg actgagcgta acctgattct
     1081 gacccagtaa tgcattaatt ggcgttctaa gttcatgagc gagatcgtca gcaaattgac
     1141 taaggcgctc aaaatctttg actaaagcat gatgcatttt attcaacgcc tgcccaagag
     1201 gttttagttc tctcggtaat gcgttaacct caaccggcac tcggctatcg ttataattca
     1261 gcgcttccgt tacgccactc aactttttga tctctcgcaa ccccgtcctg attaacaacg
     1321 gacttaatac tgagcacagt ataatggcga caatgcaaat tataatgcta ttaattttat
     1381 actgttcaag catattatgt ctggctgaag ccaatttagc cacagtaacc gttaatgatg
     1441 gcgaaacttc atcaatgtta attcgtaaag catctatctc tgtatcatta ataatgcttc
     1501 tgtaaatgcc tgctgcgctg attgtctcac tagcaggtat gttatttaac atgtcatcac
     1561 tgacatttgt ccggttaaca attttattga tgccatcact atgaatgatc aaaatatcct
     1621 gactaacatc catcatccgg ttaaagtaca caggtaacgt atctggattt accccatcaa
     1681 ttaacaactg cttgatctgc gctgtccggt taatgagtgt tgtatcatcg cgccatttca
     1741 actcacttgc caggccatta tagagagtcc atacaattcc agcgccagca acagactgca
     1801 gaaatataaa aagcaaggtt aaacggacgg ttatagataa tcttttcatt atttttttac
     1861 cgctacgaat gaatagccca tcccccggat tgtggcgatt agcttttcag gaaaaggatc
     1921 atcaactttt gcgcggagcc tgcgaatggc gacatccacc gtattggtat cactatcaaa
     1981 gttgattccc caaatttcac tcgcaataac cgttctgggt ataatctcgc cagctctgga
     2041 ggccagtagc caaagtaact gaaactcctt gcgcgtcagt gtaatactga tattgtccct
     2101 gctgacactt tgactgacag agtccatttt taagccactg atttctaatg ttgaattcag
     2161 agcgtgatgt tgccttaatt gtgcccgaac ccttgctaac aactcagaaa atgaaaaagg
     2221 ttttaccaga taatcatttg ccccactgtc caaccctctg actctgtcat cgacagaatc
     2281 ccttgcagta aggcaaataa caggggtttg ctttgccgtt cttaacgttt gtaagatctg
     2341 ccagccatcc atacccggaa gcataatatc cagaatgatc aatgcataat catccttcag
     2401 cgcaagataa agcccatctc tgccatcaga aacggcatca atgacataac ccgcttcgga
     2461 aagcccctgc gttacccatt cctgggtcct ttgattatct tcaataagta gaatcttcat
     2521 gaaaataata tgccataaat catatgttat agaaacaacc tggttcatta caaaattgta
     2581 atgctgctgt aaggttaccc tggacgtttt ttcgctatcc tcaaaactca ttcacatgac
     2641 aaggatataa acatgttaaa gcgttattta gtactctcgg tagcaacggc agcattttca
     2701 ttaccttctt tggtttatgc cgcacaacaa aacattctta gcgtgcacat tttgaaccag
     2761 caaacaggaa aacccgctgc cgacgtgaca gtcactcttg aaaagaaagc ggataacggc
     2821 tggttacaac ttaataccgc caaaacagat aaggatggac gaattaaggc actgtggccc
     2881 gagcaaactg cgactactgg cgattaccgt gtcgtattta aaaccgggga ctatttcaag
     2941 aaacaaaatc ttgaaagttt cttccctgag attcccgttg aatttcatat taataaagtg
     3001 aacgagcatt atcatgtgcc tttgttactc agccaatatg ggtattcaac ctatcgtggc
     3061 agttaatgcg taaatatgta aaagctgatc tctgctactg ttagggaggt gactccctga
     3121 cagattaaca gtaaacagct cttgttggtt aacgacaaaa aagtgtgatg gcttatgaaa
     3181 aagaatcaat ttttaaaaga atcagatgtt acggccgagt cggtattctt tatgaagcgt
     3241 cggcaggtgt taaaagcact gggcatcagc gcagctgcat tttctttgcc tcacgctgcg
     3301 catgccgatc tgcttagctg gtttaaaggg aacgatcgtc cgcccgcccc cgccggaaaa
     3361 ccgctggagt tcagcaagcc tgccgcctag caaaataacc tgccactgac gccagcagat
     3421 aaagtctccg gttataacaa cttctatgaa ttcgggctgg ataaagacga tcccgcggct
     3481 aatgctggta gcctgaaaac cgatccatgg acactgaaaa tcagcggcga agtggcaaaa
     3541 ccattgaccc tcgatcatga tgatttaacc cgtcgcttcc cgctggaaga gcgtatttat
     3601 cgtatgcgct gcgtggaagc gtggtcgatg gtggtgccgt ggattggttt tccgctacac
     3661 aaattgctgg cgcttgccga acccaccagc aatgcgaagt atgtcgcttt cgaaacgatt
     3721 tatgcgccgg aacagatgcc tggccagcag gaccgcttta tcggcggcgg gctgaaatat
     3781 ccttatgtcg aaggattgcg tctcgacgaa gcaatgcatc cgctcacact gatgaccgta
     3841 ggtgtttatg gcaaggcgtt accaccgcaa aatggcgcgc cggtgcgact gattgtgccg
     3901 tggaaatatg gctttaaagg gattaaatcg atcgtcagta ttaagctgac ccgcgagcgt
     3961 ccgccaacca cctggaatct ggcagcgcct gacgaatacg gtttttacgc caacgttaat
     4021 ccgcatgttg atcacccgcg ctggtcacag gctaccgaac gatttattgg ttcaggcggc
     4081 attctcgatg ttcagcgcca gccaacacta ctgtttaatg gttacgccga ccaggtggca
     4141 tcgctgtatc gtggcctgga tttgcgggag aatttctaaa tgcgtctgac agcaaaacag
     4201 gtgatatggc tgaaagtttg cctgcatctt gccggattgt tgccgtttct ctggctggtc
     4261 tgggcgatca atcacggtgg actgggtgcc gatccggtga aagatattca gcattttacc
     4321 ggtcgcactg cactgaaatt tttgcttgcg gccttgttaa tcacacctct ggcacgctac
     4381 gcaaaacagc cgttattgat acgcactcgc cgcctgttag gcttatggtg ctttgcctgg
     4441 gcgacactgc atttaaccag ttacgcattg ctggagttgg gcgtgaataa tctggcgtta
     4501 ttaggtaagg agttaattac ccggccttat ttaacgttag gaattatcag ctgggttatt
     4561 ttgcttgctt tagcgttcac ttcaacccag tcgatgcagc gaaaactcgg caaacactgg
     4621 caacagttgc ataacttcgt ctatcttgtc gcgatcctgg ccccaataca ttatctgtgg
     4681 tcagtgaaga ttatttcacc gcagccactc atctacgctg ggctggctgt actgctttag
     4741 ccttacggta taagaagttg ctttcccttt tcaaccggtt acgcaagcag gcccacaaca
     4801 aattatctct ataacttcat attgccgaca aacttcgcgt tatcccggcc ggatgcggcg
     4861 cgagcgccct atccggccca cacagatcgc atcaagttat gtttcgtttg tcatcaacct
     4921 ttcatcacca gaataattta ttttatttca ataagatagt tcaatacacc tgagaatacc
     4981 atgctctcat ttcctaatga gttgttgcat tttttcatat gttacaatat aacattacac
     5041 ataatgtaca ttaactctgg aggaaattgt tttggcgatt cgtcttcata aactggctgt
     5101 tgctttaggt gtctttattg ttagcgctcc tgccttttcg catggtcatc attctcatgg
     5161 caaaccctta acagaggtcg aacaaaaagc tgctaatggt gtttttgatg ataccaatgt
     5221 acaaaaccga acgctcagtg actgggatgg agtctggcaa tccgtttacc ctttactgca
     5281 aagtggcaaa cttgatcccg tctttcagaa gaaagcggat gcagataaaa ccaaaacatt
     5341 tgctgaaatt aaagattatt atcgcaaagg ttatccgaca gatatcgaaa tgattagcat
     5401 tgaggacggc attgtcgaat tccatagaaa taatgaaaca acatcctgta aatatgatta
     5461 cgatggatac aaaatactca cctataaatc aggcaagaaa ggcgttcgtt acttattcga
     5521 atgtaaagat cctgaaagca aagcccctaa atatatacaa tttagcgatc atataattgc
     5581 accacgaaaa tcatctcatt ttcacatttt tatgggtaat gattcccagc aatctttatt
     5641 gaatgaaatg gaaaactggc caacgtatta tccctatcag ttgagtagcg aagaagtggt
     5701 cgaggagatg atgtctcatt gatttttttg cgctgacttc agttatttaa cagaacagtg
     5761 cctggtagta acaacaataa tttaccaaga caaataagta agacaaactc agacacgctc
     5821 atacttgagt cgtgtctttt ttattatgtc tgtagaaaaa aactgagaaa aaggcgaaca
     5881 ataaatacac aactcagcca tacagactgg cagcgcaaat gtttccagga gcaagtcatc
     5941 tgtcatggta aagctaacca gaaatatagg ttaattttgt tgtttaaacc ttttttactt
     6001 gttagtataa tacaaaggct ttcaaaaaag ctgggaaatt caacaactct tggaagttat
     6061 ggactttgtc ccgcggcttt aatctgacgg ccgcgttcct tttttggctc aattattgac
     6121 cccttctcta cgcaacttca gttcccgcca gcaactttgc ggcagtttca taggatcaat
     6181 gtctaaaaga ataatggtga ccgataagaa aacgactgaa taactgtaga ttttcgctcg
     6241 aaaccttcct gtcagctcca tagcgaatca agcgctgaat gtcacagtat cgaacagaaa
     6301 acagtgacga tctaaccctt caagaatatt ctacgattgt tctgtttagg aaaagcaagg
     6361 cgggaagtcg ggagataagt cattgataaa gtggcggaga gagggggatt tgaccccccg
     6421 gtagagttgc ccctactccg gttttcgaga ccgatgatac agagtcgaaa aatcaaagag
     6481 ttaaaaattt ttcttggaaa aaatgtctta aaatcgtaca cgaaaaatca atgaattaca
     6541 taacattaaa gaagaatatt ccaagccttc ttgagtccac cacctgtttt caggtgtcat
     6601 tcagacgtga tttgccccag attgggaaag cacgcttttt tctcagctta ttccgttttg
     6661 cctgtgtgtt gaataattcc actgcagtta tataaaatcg gtaacggctg gaaatcattc
     6721 aatactcgca ctatcgaaag tttaccagcc aaccgcagca cgtcctgcat acgtcgtgtc
     6781 tgcggttttt ctttttcgct tacattgtgt ctggttcttc cggccactca atatcaggtg
     6841 cagttgatgt atcaacacgg ttcagcaaca cccgatactt cttccaggct tccagcaacg
     6901 agttttct
//
LOCUS       DAJTXY010000166         6771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTXY010000166 DAJTXY010000000
VERSION     DAJTXY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.167"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..3513
                     /locus_tag="OO566_003778"
                     /pseudo
     CDS             1..3513
                     /locus_tag="OO566_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3523..3766
                     /locus_tag="OO566_003779"
                     /pseudo
     CDS             3523..3766
                     /locus_tag="OO566_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            5305..6771
                     /locus_tag="OO566_003780"
                     /pseudo
     CDS             5305..6771
                     /locus_tag="OO566_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1794 a         1779 c         1630 g         1568 t
ORIGIN      
        1 cgaaagataa ttctttgcaa ccttcccaaa cggtaacgga ctctctcaag gtagcggact
       61 tatccaccat tattcaggaa caaccctctt tcgtagcaga aacccgtgcg gctgacaaaa
      121 atacagatgc tgttttacct tggctggcga aagatattgc tgtcgttttt ccgcccgaag
      181 ttgtacacac cacgttaagt catcgccgct ttcccggagt tcctgttcag caagcagaca
      241 aattgaccca attacgtcgc ttagcctgta gcgtgtcgca acgggataat aaaacagcca
      301 catttgactt cagcacctgt tctttagaat ggcaaaacac cgtcgcccaa gcgatcagtc
      361 agatcgacgg cctgaaaaca acacagctac catcaccagt aatggctgta ctcacggcac
      421 ttgaaatgaa atgcacaaga tataaagtgc gtgaggatgt tatggatcag atcgtccagg
      481 aaggcggtct ggaatatgct actgatgtaa taatacacct tcaacagatt gatattaaat
      541 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
      601 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      661 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      721 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      781 gtctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      841 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      901 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      961 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
     1021 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
     1081 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agaacaggtc attccctggc
     1141 tttcgacacc cgcagttgca gtgctgaaat catgccagca ccaactgaca cagccctcaa
     1201 accatgccag cgccgatcta ctgccagcca tagtagtctc cccaccatgg atttcgaaaa
     1261 agaaaaaatc gccgattccg gtgctggatt tagcgccatt aggcatcgag ccaatctgtt
     1321 atctgacaga agaaatcagt aatcaacttt tggcgaaata tatctggtat tcaaaacaca
     1381 tcacggttag ccatgaagaa agtactacca acctgttggc aaggatgggt tttcaacgac
     1441 ggatcgctgg tacatatatt aaagctcccg aagcggtagt tgaggcatgg ctaaatgaag
     1501 attattcaac cttactaagt gaatttaagg tgtttcattc acctaccggg cattattggc
     1561 agttggggat tttgacaaca ttgccgctgg aggctggaga aagcagtaaa agcatggaat
     1621 gcccttactc tatctccaca taccgatacc gaatacgcca tgttacattt tggactcaaa
     1681 ggtttacctg gtttagttaa ctcaattaca cgctatccgc aggaagcctt gcccatcacg
     1741 aattacttcg cagcgagtga gctggctcct gccgtcgccc gtgccttcaa caaactcaaa
     1801 acgctacgcg aaaacgcccg cacctggctg ttgaaatacc cggaacatgc ccttaccggc
     1861 ctgctgcctg cggcgctcgg caaagccggt gaagcacaag ataacgcccg cgctgccttg
     1921 cgtatgctta ccgaaaacgg tcatcagcca ttactgcaag aaatcgcccg gcgttataac
     1981 cagtcggaag taacagacgc ggtgaacgct ctgcttgcgc tcgatccctt agataatcac
     2041 ccgacaaaaa tccccactct tccgaccttt tatcagccat cgatctggac gcgcccggta
     2101 ttaaaagcaa acgcccaatc actgccagat aacgccctcc tccacctcgg cgaaatgctc
     2161 cgcttccctc aggaagaggc tctgtatccg ggattattgc aggtgaaaga cgcctgtacc
     2221 gctgactctc tggctgaatt tgcctgggat ctgtttaccg cctggcagac cgctggcgcg
     2281 ccgtcgaaag agagttgggc gttcactgcg ttaggcgttc tcggtaacga tgacaccgcc
     2341 cgcaaactga cgccattaat acgcgcctgg cctggtgaat cccagcataa acgcgccacc
     2401 gttgggttgg atattctcgc cgctatcggt agtgatatcg ccctgatgca gcttaacggc
     2461 attgctcaga aactgaaatt caaagcatta caggagcggg caaaagaaaa gattgccgac
     2521 attgccgaga gccgcgaact cacggtagcg gagcttgaag atcggttagc accggatctc
     2581 ggtctggatg ataacggttc gttgctgctg gattttggcc cacgccagtt caccgtcagc
     2641 tttgatgaaa ccttaaaacc gtttgtgcgt gatgtttccg gcagccgcct gaaagacctg
     2701 ccaaaaccaa acaaaagcga tgatgagtca caggcgaacg atgctgttaa ccgctacaaa
     2761 ttgctgaaaa aagatgcgcg taccgtcgcc gcccagcagg tagcaaggct tgaatccgcc
     2821 atgtgcctgc gtcgccgctg gtcgccggaa aacttccaac tcttcctggt tgtgcatccg
     2881 ctggttcgcc acttaacccg ccgtctgatt tggggcgttt atagcgccga caaccagcta
     2941 ctggcctgtt tccgggtagc ggaagataac agctacagca ccgctgacga tgatcttttc
     3001 accctgccgg aaggcgatat ctctatcggc attcctcacg ttctggaaat atcaccgacg
     3061 gatgctgtcg cctttggtca gctttttgcc gactacgaac tgctaccacc gttccgtcag
     3121 cttgaccgta acagttacgc cctgacagaa gccgagcgca acgccagcga actgacccgc
     3181 tgggcaggca gaaaatgccc gagcggccgg gtcatggggc tggcaaataa aggctggata
     3241 aagggcgaac cacaggatgg aggctggatc ggctggatga tcaaacctct cggtcgctgg
     3301 tcgttaatca tggaaatcga cgaaggtttt gcggtgggta tgtcaccagc cgaacttagc
     3361 gctgagcagc tcttaagcaa gctgtggcta tgggaaggca aagcggaaag ctatggctgg
     3421 gggagtaatt caacacagga agcgcagttc tccgtactcg atgccatcac cgccagcgag
     3481 ctaattaacg atattgaagc cctgtttgaa taaggaaaga gcatggacaa ggaattaccg
     3541 tggctggcgg ataacgccca actggaactg aaatataaaa aaggcaaaac accactcagt
     3601 catcgcaatt ggccgggcga accagtgcct gttatcactg aaagcatcat tcagacactg
     3661 ggtgatgaat tgctacaaaa agctgagaag aaaaaaaaca ttgtctggcg ttatgaaaat
     3721 ttttcaccgg agtggcagtc cgccattacg caggccatca acttgatcgg cgaacacaaa
     3781 ccctcagtcc cggcccgcac aatggcggca ctagccttta tcgcgcaaaa tgacaaccaa
     3841 tagttgctcg acgaaatcgt ccaacaagag gggctggaat atgcgactga ggtggtgatt
     3901 gcacgccagt ttattgcacg gtgttatgag agtgatcctc tggtagtcac attgcagtat
     3961 cagaacgagg actatggcta tggttatcgc tcagaaacct ataacgaatt cgatctccga
     4021 ctgcgtaaac acctctctct ggcagaggaa agctgctggc agcgttgcgc cgacaaactc
     4081 attgccgcac tgccaggaat aaccaaagtt cgccgccctt ttattgcgct gatcctcccc
     4141 gaaaaaccag aaatcgccaa tgagttggta agccttgaat gcccgcggac tcattttcat
     4201 tctaaggagt ggttaaaagt tgttgctact gaccccaaag cggtgagaaa actcgaacgc
     4261 tactggagcc aggatatatt tagcgatcga gaagccagct acatgtcgca tgaaaaccac
     4321 ttcggctacg cggcctgcgc tgcccttttg cgcgaacaag gactggcagt cgttccgcgc
     4381 ctcgcgatat atgcccataa agaagattgc ggcagtctgc tggtacaaat taaccatccg
     4441 caagtcatcc gcaccttgct gttagttgct gataaaaaca aacccagcct gcaacgtgtg
     4501 gctaaataca gtaaaaactt cccccatgcg acactcgccg cactggcaga actgctggcg
     4561 ttaaaagaac caccagcccg cccaagttat ccaatcatcg aagacaaaaa gctgcctgca
     4621 cagcaaaaag ctcgagatga atactggcgt acgctgttac aaacgctgat ggcatcgcag
     4681 ccacaactgg cagcagaagt gatgccgtgg ttaagtactc aagcccgggc agtgctgaat
     4741 agttatttat cggcaccgcc caaaccagtt attgatagca ccgataacag ccaaatgcct
     4801 gaaatcctcg tttcaccacc atggcgtggg agaaagaaaa agacttccat acgtcacttt
     4861 tcgctgcctg atcttacgct tgcaccgcaa acgtacaggt cgccgaataa acactggacg
     4921 ccggaacagc aaactgcaat tgaccatttt agcgccatgt cttttgacga acgtcttgct
     4981 acgagaggag cggatatctt cttgcgggaa ttaggttttg acgttcatag cctggaattt
     5041 aataagcgtc ttcttaatgg acagttggat gcctttgatg ctcagcaact gcaagaatac
     5101 acatcaatag gactattgcg tacttatcac gcgcatcagg ccaaatctaa acagtaccgg
     5161 gaagacgcca ccaatgcatt actggcacaa gatagcattg cgttgatggg agggtggcga
     5221 acttttcagc acggatttta ctatggtaat gaacagcgtt gggggatttg gaatctgtac
     5281 cttatcgcgc agatgccgcg cgaaatggcg gtatcgtgct ggcaacaaat tgtcgaggct
     5341 gattttcatt acaccggggt ggaatatttg ttaagtgtgc tgggtaccga tgctttgcct
     5401 ggattaaaca ccgcctttgc ccgacatcca aaagagatat ttccgctgct gattcatttc
     5461 ggcgcaacag aactggccct gcccattgcc cgcgtctggc acctttttgc gggccagcgt
     5521 aatctggctc gccagtggat tttacaatgg ccggaacata cggctactgc acttatccct
     5581 cttgtcttca ccaaatctag cgataaaagc gaagctgcat tacttgccct gcgtttactt
     5641 tacgaacatg ggcatggcga attgctacaa actgtagcca atcgttggca gcgtacagat
     5701 ctatggcctg ccctggaaca attacttaaa cagagtccaa tagagattta cccggcacgc
     5761 attccaaaag cccctgattt ctggcatccg caaatgtggt ccaggccgcg ccttatcact
     5821 aataatcaac ctgttaccga tgacgctctg gaaattatcg gcgaaatgct gcgctttacc
     5881 caggggggac gtttttatag cgggctggaa caactgaaaa cgttctgcca gccacaaacg
     5941 ctggcagctt ttgcctggga tctcttcact gcgtggcaac aagctggtgc ccccgcaaaa
     6001 gacaactggg catttctggc gttaagtctc tttggtgacg aaagcacggc acgggatcta
     6061 acgacacata tcctcgcctg gccacaagaa ggcaaatctg cccgtgcggt cagtggcctg
     6121 aacatcctta ccctgatgaa taatgatatg gcgctgatgc agctgcatca tatatcgcaa
     6181 cgggcgaaat cccgcccctt acgtgataac gcggcggaat ttcttcaggt agtcgcagaa
     6241 aatcgcgggc taagccagga agagttagcg gacagattag tcccaacact gggccttaat
     6301 gatccgcagg cgttgagttt tgattttggt ccccggcagt ttaccgttcg cttcgatgaa
     6361 aacctcaacc cggttatctt tgatcagcaa aacgttcgcc agaaaagtgt tccccggttg
     6421 cgcgccgatg acgatcaact gaaagcgccc gaggcactgg cccgactaaa agggttaaaa
     6481 aaagatgcta ctcaggtgag caaaaacctg ctcccgcgtc ttgaagctgc cctacgtacc
     6541 acccgacgct ggtcgctggc agattttcat tctctgtttg ttaatcatcc ctttacccgt
     6601 ctggttaccc agcgattaat atggggggtt atccggcaaa tgaaccgcgt cgtttactca
     6661 acgcctttcg tgtggccgca gagggggagt tctgcaatgc gcaagatgag ccaattgacc
     6721 tgcctgcgga cgctctgatt ggcattgccc acccgttaga aatgacagca g
//
LOCUS       DAJTXY010000167         6710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTXY010000167 DAJTXY010000000
VERSION     DAJTXY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.169"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            204..280
                     /locus_tag="OO566_003781"
     tRNA            204..280
                     /locus_tag="OO566_003781"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:238..240,aa:Arg,seq:tct)
     gene            complement(326..1087)
                     /gene="envY"
                     /locus_tag="OO566_003782"
     CDS             complement(326..1087)
                     /gene="envY"
                     /locus_tag="OO566_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6606876.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINYYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1270..2160)
                     /locus_tag="OO566_003783"
     CDS             complement(1270..2160)
                     /locus_tag="OO566_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6606877.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2161..5139)
                     /gene="nfrA"
                     /locus_tag="OO566_003784"
     CDS             complement(2161..5139)
                     /gene="nfrA"
                     /locus_tag="OO566_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCR6606878.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDP
                     ALIRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVG
                     RRWTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLS
                     VYSRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTML
                     RASASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANG
                     QTIEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFK
                     AINQRNGERNNAFLTIGVHW"
     gene            complement(5126..>6710)
                     /gene="nrfB"
                     /locus_tag="OO566_003785"
     CDS             complement(5126..>6710)
                     /gene="nrfB"
                     /locus_tag="OO566_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR6606879.1"
                     /translation="HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQ
                     SLTEDYDIGFRLKEKGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTF
                     STAVRQKSRWIIGIVFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLL
                     LLLAYESLWPDAWHFLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLL
                     SVLRLFWGNLINFMANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQIL
                     LENQVITEEQLDTALRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQNGVAWESIDAWQ
                     IPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIV
                     LRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILT
                     TLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLL
                     KAGLNTEQVAQLESENEGE"
BASE COUNT         1535 a         1763 c         1770 g         1642 t
ORIGIN      
        1 gcggtaaatt gtactttgat acatgaaaat acgggttttc ttgattcaga cgcgcagcgg
       61 tgtgcgtttg tttgccgcta tagcgaaata aatcagaaaa tcagacgcgg tcgttcactt
      121 gttcagcaac cagaccaaaa gccattgact cagcaagggt tgaccgtata attcacgcga
      181 ttacaccgca ttgcggtatc aacgcgccct tagctcagtt ggatagagca acgaccttct
      241 aagtcgtggg ccgcaggttc gaatcctgca gggcgcgcca tttcttcttc atttatgccc
      301 gtcttatccg tttccgcttt gcccttcacc acatcacttt ttgtcgctgt ttggcgagat
      361 aattcaacgg tgtcaggctg taaaacgcct taaaaacaga gataaagtac gacgtgctgc
      421 tatagccaca taattgcgcc acctgagtga tatttttgtt atccatcaat aacatctgta
      481 cggcgtaacg catacgacac tctgtgacaa tctggctata gctggtattt tcgtttttta
      541 atttcttttt gagcaggctg gggcttaaac atagcgaact ggcgacaatt cgcagattcc
      601 agtaatgctg aatatcgctt tgaataatgc ggcagacgct gtcgcggacg ctgctgcgta
      661 agatatacat cagtagggca ataaaccgcg attgctcaag aaagttagac aatacggtaa
      721 aaagtaatgc gcgcgtcaac tccgcttcgc agggtttgct gggttgctgc ttgctgtggt
      781 tggcggctag ccggaacact tccggtgtac ggctatgaca agcgataacc ggggttgcca
      841 gccgcgacca gggctttaca caggttaatt tcttatttaa aaacaacaga taatagttga
      901 tgatgttacg gttgatgtgg gtgattaata cgtgatttaa cgttgagagt tcaatgacgt
      961 tattgttgca cgcgaaggcg gccaggtagt ttttcggaag ggtaaacgta gcatggttat
     1021 tgacgcttac ctctacctct ttttcggtca ggatcaccac gcaaggttca ctgctgctca
     1081 attgcatttc gcactcctca gatatcagaa actccgctca aaggatctat gcttcctgca
     1141 tgagtgatcg gcccgttcgc cgataacgat cttctttctt tagcatgctt tttagcaatt
     1201 aatcttgatg gaattctgac gagagcgaaa gaggtaagcc aggtcgtacc cgacttacct
     1261 ggaggggatt taatactcga gaatgccgtg cgcgacgggc aaatagcgca gagagaaata
     1321 gagcatgtct ttaccgcaag aggaacgttt cgctaacagc gaggcaattt ctgcgtccgg
     1381 tttcggtttc gctgtaaagg ttttgccttt gccggcgaca aacagttcat aaacaatgcc
     1441 gcttgctggg gcggagcttt ggcaatcggc gctggcctgt agataacgtt cacgctgttc
     1501 agcggtaagt ttatccacgc cgctgccatc ctgtacccag acattaactc cggttgcttt
     1561 aacttgttcc agcagttggc gatagccatc gggcgacatg tttccggcga aaaaactact
     1621 gatataaacc ggttttgctg aaacatcgct aatcagccgc tgcgcgttgt ttaaccatgt
     1681 tagcaaaggt tgacgggcgg cttcgctgcg ccagttcagg tcgtcaattt ccgcgctgat
     1741 gtaccagcca tccggcgtta tgccaggcgc ggcgctccat aatctggctt gctggagatc
     1801 ggcagccagc aggcgattaa gatagctttc cagcgctgca gacgactgtt tctggtgcat
     1861 aaaaaattcc ggatcggcgt tcagcccgac aatcagcttc aggccagtct gttgcgcagc
     1921 tgcggcacgc ttaaacaata acgtgcgctg ttctggctgg gtaaatgcat cgccgtaacg
     1981 ggtccattgc aaaaccaggg tatcgaagcc ttgaaaacgt aactgactca tcagcccctg
     2041 ccactgggta tcggtaacct gactatctcg gttttgtggt tgccagataa tacctttcat
     2101 cgcaaaggaa aaagggctga ccaaaagcag tgtcagcaat acgaaaatga acttacgcat
     2161 ttaccagtgc actccaatgg tgagaaacgc gttgttgcgc tctccgttac gttgattaat
     2221 cgccttaaag gtatgttgat actcgacgcc gagactgact ttgtgcggcc aggcgtcgta
     2281 gtgcgtctcg ccggtccaaa tattccagcg gaccccgaca ccgccaagct gcgcgccctg
     2341 agtgccttta tcacgatagc cgttgtcctg aacgtgagcg taaggctcaa tagtctgtcc
     2401 gttagctacc ttctgatgcc agctgacgcg ataatctgcc gtccacgcct gaatatcctg
     2461 gcggatatat tgcgccgcat cgaggtacag gttttgggca aaccagcctg aaccgttcgg
     2521 gtgccattcg tcgctgtatt tgccgccatt aaagaatgag gcgctggcgc gcagcatggt
     2581 atcggatgcg ccattttgcc cgttcagcgg caactgctgt tcgacggcga ggaaaaagat
     2641 ctgatcgcgc agcggcttcc agcgcagacc ggtgccggac atcggatttt tcaccggcat
     2701 catcaccccg ttttctccgg tatcggcaaa gacgcggcta taaactgaga gcaggtcgcc
     2761 ttccagcagc atattgcgtc cgatgcggta ctcggcttcc agttgtccgt agctacgata
     2821 gcttttccca ggcgctgcac cgccgacatt attgttagcg gtactcattg cgccggaacg
     2881 caagccgatg gaagaatcga aactgaacgt ccagcggcga ccgacctcct catgcaaacg
     2941 gcggaaattg aagcgttgct gattttgctc tggggtcagt ggggttatca gcgcctgatt
     3001 atcaatgtca tcaatcacca gccgggcgta gtgctgcgtc gcaggcatgt catccagacg
     3061 ctggttcacg taggccagtt gtcggatcag tgccggatcg tccggaagcc ctttatgcgc
     3121 ctgttcgagc atttcccgcg actgtgcgat atcaccgcta tcccacaagg cgtaaccaag
     3181 cgctgcctgg gtgttgctat tattcggttc cagttccagt tccagcgcgg cgcgcaaatc
     3241 actcaccgcg gccggaacat tatgacgttg gcgataaatt gtcgcccgcg caacgtaagc
     3301 gttggcagaa ggggcaatat tgattgagcg cgtgagatcg ttcagtgcga gttctggctg
     3361 agctgggata taacgttgcg catgcagcca ccagtattga gcattgtttc ccagctcacg
     3421 ttgttctgcc tgttgaagcc agcgatcgcg agccgcgcca tttcccgctg cctgagcggt
     3481 gttggcggcg gcgagcagat cctcattgct catgtcgtga agactgattt tctgccaggc
     3541 cgccagtgcg gtggcgtagt cctcaacctg atacgcctga taggctaccg cacgatgttg
     3601 ccaggcgctc ggttgtcgtt gttcggcctg aagccatgca tacaacgcca caccgggtag
     3661 cgtgtcccga taacactttg ccagacggtt ccaggcggca gcatcgtagg aaggcgacat
     3721 atcgcccagc aagcgaacta ttgccgggca attatctgca atacccggca actgactttg
     3781 ccactgacgt tgctccgcca gcggtaaggg tttcgataaa atcgccacct tcgccggcgt
     3841 tgctaggtaa ggatgacttt ccagcagaga cgccagtcgc gccattaaag tctggctgac
     3901 acgcgcatcg ccctggaaag gatagcgttg cagcaataaa tcggcagctt cgcgtgactg
     3961 ctcgttctgc atcagttgcc aggtcagttg atccaggcgg ggaagatttg tcggttcttg
     4021 ctgatacagc aatcgtgcca gacgcagaga ttcagccttg ttacgggtcg ccacgctgac
     4081 agcataacgc tcctcaagca tttcattggc ggggagggtg gcgagcagtt tttgcgctgc
     4141 gtcgtactga ccctctttta acagcaccgg tagcgtcgcg ccaacaacat actggcggtt
     4201 atcggcaaac tgcaccgtat aattcgccaa tgcctgaacg gggttggcgc tgtatttaga
     4261 taacagatag agccaacttt tctcttgtgc gcccgtggta aacagcggct tattttcaat
     4321 gagataatgc tggaggcgtg ctttttcgcc acgataagcc agcgcggtcg cgtaagtaat
     4381 atatgactga ggatcggtga agatcccctg tgattgcagt gccaggatcc gatcgtccag
     4441 ctgcccggca agaagcacgt caaaccactg acggcgttct gccgcgctta atgtgttctg
     4501 ctggcgtgct tcattgtata gcgtatctgc ctgggaccat tgtttcaggt agattgcccg
     4561 ttgcagcaga tcggttcgca gcgtttttcc ttccggcgat gcagcaaacg tcgcatcgtt
     4621 cagttgcgct ctggcgacag gtaattgtgc cagccgcagg gcattctgcc cgacttcact
     4681 gcgacaacgc agggtcggcg cagcgtcgcc cgctttttgc tgggcaagca gttcttcaac
     4741 agtcgtaacg cttttcactt caactggaat agccgccaga ctgcgctcaa gtcgggcatc
     4801 tcctgggtga cgtttcagtt gatcctcaag caacagccgc gcccggtcat catgaccaaa
     4861 atgacgatag gcttccgcaa ggtataaagt cagcggaata ttatccggca cctgttggtg
     4921 tatatattca aattcgcgga tggcggttgc ttcgtcgtta tttttctgtg ccttcagcgc
     4981 cttatcgaga cggggataaa taacaaaatg gcgataatcg ctcagcccca gctcttctgc
     5041 gctggtgccg atattgtctg cgagtgcgct ggtactcaat aaagacgtca gcagtaaacc
     5101 agaccatccg atgacgcgat taaggttatt ctccttcatt ttcggactcc agttgcgcaa
     5161 cctgttctgt gtttaaacct gctttgagta atagtgattg catcgaaact tgtaattcgc
     5221 gttgaattgt caggacgcga tccaacgttt cctggctgat aacgccttcg gtgaccaaaa
     5281 acttgccgag cggcagagaa ctgcgttcat ggcgcaataa caacacgtta attgctgaac
     5341 gattaatatg accgagcgtg gtcagtattt cggcgaacag gaactgatgc ggcacatatt
     5401 gccgccagat ttcaccggtc tgctgttccg tgagccactg atgctgaacc gcattgtaca
     5461 acattgcccg cggatcgtga ccgcgtcggc gtgcatacca gtggcgtaac cctgtgacaa
     5521 tttgtccccg cagaacaatg acgtaacgca ctttgcgtcc gactttacgc gtcagggccg
     5581 ccagcgaaac cgggtcaata ccatcttcac tgccgacaat taactcatca ttttccagac
     5641 gcagcggcag tactgcataa tgcagcgcta cggaggctgg catttcggca atcagcgagg
     5701 acgggatctg ccaggcatcg atggattccc acgccacgcc gttttgctct gcctgcgcct
     5761 gtgccagctg ctcggcgcta atcagcccct gcatcagcat tgaaccgccc aggcgtagac
     5821 cttcgacgcg attacgcagt gctgtatcga gttgttcttc agtgatgacc tgattttcca
     5881 gcagaatttg acctaacggg cgcaacgagc gggtatcgcc agtgacgctg gggaagtcat
     5941 gcgttgtttt atcccacgcc acgcgacgcg gatcgccgtg ctgaagaacc tgtttcagca
     6001 cgcgccagtt agccataaag ttaatcaggt tgccccagaa aagacgcagg acggaaagca
     6061 ggccctgcgt gaggccgtag tagccagtga cgaaaatcac tcgctgcacg atgcggttaa
     6121 ccatcaagcc aaagtttagc cacagcaggg tcattaacca tgcgctgccg ctaaaaatag
     6181 aaaggaaatg ccaggcatcg ggccacaaac tttcatacgc cagcaacagc aaaagctgga
     6241 tcatcaccag catcgcgagg aagctgacaa agttactgat tgcccctttg cggtcgcgcc
     6301 agagaaagta gttcagcgtc aggctggagg tccatttatg ggttttaaag ccctggaaaa
     6361 caatgccgat gatccagcga gatttttgtc gaaccgcagt cgaaaaggta tcggggaaat
     6421 attcgcgcac gcagatcatg tttgacgtcc gtgcgtgctg taaaaattta cgctgctcgc
     6481 gttctttggc ttcgtccacc accggaaaac ggacaaaaat ttccgtcata cctttttctt
     6541 tcaggcggaa gccaatgtcg taatcttcag taagactctg cacgtcgaaa gcaataccgt
     6601 taccgtcagc taacagtgcg gtcacggcgc ggcggctgaa acaggtgccg acgcctgcgc
     6661 tgggcacttg tccggcgagg gcttcacgca ccggaacatc tttgccatgc
//
LOCUS       DAJTXY010000168         6629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTXY010000168 DAJTXY010000000
VERSION     DAJTXY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.170"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            229..2661
                     /locus_tag="OO566_003786"
     CDS             229..2661
                     /locus_tag="OO566_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6606880.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDILCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2728..4188
                     /locus_tag="OO566_003787"
     CDS             2728..4188
                     /locus_tag="OO566_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6606881.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGK"
     gene            4197..5858
                     /locus_tag="OO566_003788"
     CDS             4197..5858
                     /locus_tag="OO566_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6606882.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6079..6420
                     /gene="symE"
                     /locus_tag="OO566_003789"
     CDS             6079..6420
                     /gene="symE"
                     /locus_tag="OO566_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6606883.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6467..>6629)
                     /locus_tag="OO566_003790"
     CDS             complement(6467..>6629)
                     /locus_tag="OO566_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606884.1"
                     /translation="ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAF
                     EKQMESALV"
BASE COUNT         1959 a         1572 c         1519 g         1579 t
ORIGIN      
        1 aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgt ttcctgtaac
       61 ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa cagagatatc
      121 aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata gccaggctat
      181 tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat ggcggaactg
      241 aacctaagta acctgacgga agcagacatc attaccaaat gcgttatgcc agccattctc
      301 aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg ggacggtaaa
      361 gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga catcgtgctg
      421 taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa acatgaaatt
      481 ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc ttttgttttt
      541 gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg tgaatgcctt
      601 gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca aaaattctgc
      661 ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta ttacgacgat
      721 ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac cattgaagcc
      781 gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg gaaaacctat
      841 accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg cattttgttc
      901 cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca gccatttggt
      961 acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat tcacctcgcg
     1021 ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca agtcgcacca
     1081 gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc cgaagacagc
     1141 gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt aaccgccacg
     1201 ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt ttacgtctac
     1261 tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt ccgtgttgat
     1321 attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt aaacggcgaa
     1381 gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt gatcgacgaa
     1441 cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa tccgatggat
     1501 aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg cgccctggtt
     1561 aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat caccggcgat
     1621 gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc gtacccggtt
     1681 atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg caaactggta
     1741 gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg tcgtggtaca
     1801 cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa aaaagccacc
     1861 gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga taccacacca
     1921 gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat cagcgaacat
     1981 gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca ggttaccgat
     2041 accgatgatg tcggcccatt tccggaagaa gacgagaaga aaatccgcaa gtttcacgtc
     2101 aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc cgacggtaaa
     2161 ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga atatgcctcg
     2221 ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct cattcacgag
     2281 ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa agatctcgac
     2341 ccgttcgaca ttctttgcca cgtagtgtat ggtcagccgc cgttaacccg caaagagcgc
     2401 gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc gcaagccgtg
     2461 ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga aagtattcag
     2521 gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat taaaaccgga
     2581 tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga aatctaccaa
     2641 ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat catgttgcag
     2701 aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa atcattacaa
     2761 gatattatgc gcaaagatgc cggtgtggac ggcgatgcgc agcgtctcgg tcagctctcc
     2821 tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact ggagcaggat
     2881 aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc caacgcgcag
     2941 ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc ggcgttgaaa
     3001 aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca ggcgttcagc
     3061 gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa caagctgaac
     3121 gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta cgaacagatc
     3181 cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg cgccgtcact
     3241 cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga cccggcctgc
     3301 ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata cgtgaagagc
     3361 gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa acagcttccg
     3421 cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt acaaattcgt
     3481 cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga tgtcattgtt
     3541 accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt tccggcagag
     3601 atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt actggcgaaa
     3661 aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg tgttaaaacc
     3721 aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg tttaccgaat
     3781 ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac caaaggtcag
     3841 ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa aaactacagc
     3901 aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg gggtaacgag
     3961 gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag cattgatgac
     4021 gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga aaccgtcagc
     4081 catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat ccagacgctg
     4141 cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaagtaggt taactaatga
     4201 gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg caaacccgtt
     4261 ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag aaattgcgtg
     4321 agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg aatgatgaac
     4381 cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg aagcaaggca
     4441 aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg tttgaattgc
     4501 cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct gagtcatcta
     4561 gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc gagcaaagcc
     4621 aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta aataatccag
     4681 ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat tttgttgtcg
     4741 gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc tttttttggc
     4801 agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa gtattaaata
     4861 gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa aaagttagct
     4921 ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg gacgcacatc
     4981 agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc gcggaactgg
     5041 ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc gaagccagcg
     5101 tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt gtgccgcagg
     5161 atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa aaagctcaat
     5221 tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc gatgaggaaa
     5281 aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata gttaatatta
     5341 aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca cctgatatta
     5401 ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca ggagtcaaag
     5461 ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc ccgtcattat
     5521 ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat ccattagact
     5581 gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt cttatgcaag
     5641 tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt ttttacaata
     5701 ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag ataaattcta
     5761 ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa acccaactgc
     5821 atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct cacaaaacca
     5881 ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc acgctaattt
     5941 tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat gatatatcca
     6001 ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca caatcactat
     6061 ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt agaagcagaa
     6121 gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta cccggattac
     6181 agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg ttttgccacc
     6241 ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc ccaacccgcc
     6301 gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct gtcggcgcgt
     6361 aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa agtcgcctga
     6421 tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat accaacgcgc
     6481 tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga agttgtgcga
     6541 ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt gtcacatctt
     6601 tggcaatacc tttcggatcg accagttca
//
LOCUS       DAJTXY010000169         6627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTXY010000169 DAJTXY010000000
VERSION     DAJTXY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.171"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..1353
                     /gene="sbcB"
                     /locus_tag="OO566_003791"
     CDS             <1..1353
                     /gene="sbcB"
                     /locus_tag="OO566_003791"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6606885.1"
                     /translation="DRPAQFAAIRTDDEFNVIGEPEVFYCKPADDYLPRPGAVLITGI
                     TPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWS
                     WQHDNSRWDLLDVMRACYALRPEGINWPENDDGLPSFRLEHLTKANGIEHSNAHDAMA
                     DVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLAHVSGMFGAWRGNT
                     SWVAPLAWHPENRNAVIMADLAGDISPLLELDIDTLRERLYTAKADLGDNAAVPVKLV
                     HINKCPVLAQANTLRPEDADRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTP
                     SDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDKRIEKLLFNYRARNFPG
                     TLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLVQQYADDKEKVALLKALWQYAEEIV
                     "
     gene            complement(1396..1623)
                     /locus_tag="OO566_003792"
     CDS             complement(1396..1623)
                     /locus_tag="OO566_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6606886.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1637..2695)
                     /gene="yeeE"
                     /locus_tag="OO566_003793"
     CDS             complement(1637..2695)
                     /gene="yeeE"
                     /locus_tag="OO566_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6606887.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALILAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(2874..4232)
                     /gene="plaP"
                     /locus_tag="OO566_003794"
     CDS             complement(2874..4232)
                     /gene="plaP"
                     /locus_tag="OO566_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6606888.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4222..4302)
                     /gene="yoeI"
                     /locus_tag="OO566_003795"
     CDS             complement(4222..4302)
                     /gene="yoeI"
                     /locus_tag="OO566_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6606889.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(4500..5429)
                     /locus_tag="OO566_003796"
     CDS             complement(4500..5429)
                     /locus_tag="OO566_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6606890.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(5475..6299)
                     /locus_tag="OO566_003797"
     CDS             complement(5475..6299)
                     /locus_tag="OO566_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6606891.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6382..6627)
                     /gene="yoeB"
                     /locus_tag="OO566_003798"
     CDS             complement(6382..6627)
                     /gene="yoeB"
                     /locus_tag="OO566_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003850603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6606892.1"
                     /translation="MWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEP
                     LKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1783 a         1741 c         1564 g         1539 t
ORIGIN      
        1 gatcgccctg cacagttcgc agccattcgc accgatgacg aattcaatgt catcggcgaa
       61 cccgaagtct tttactgcaa gcccgcggat gactatttac cccggcctgg agcagtatta
      121 attaccggta ttaccccgca ggaggcacgg gcgaaaggag aaaacgaagc cgcgtttgcc
      181 gcccgtattc actcgctttt taccgtaccg aagacctgta ttctgggcta caacaatgtg
      241 cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta atttctacga tccttacgcc
      301 tggagctggc agcatgataa ctcgcgctgg gatttactgg atgttatgcg tgcctgttat
      361 gccctgcgcc cggaaggaat aaactggcct gaaaatgatg acggtctacc gagctttcgc
      421 cttgagcatt taaccaaagc gaatggtatt gaacatagca acgcccacga tgcgatggct
      481 gatgtgtacg ccactattgc gatggcgaaa ctggtaaaaa cgcgtcagcc acgcctgttt
      541 gattatctct ttacccatcg taataaacac aaactgatgg cgttgattga tgttccgcag
      601 atgaaacccc tggctcacgt ttccggaatg tttggggcat ggcgtggcaa taccagctgg
      661 gtggcaccgc tggcgtggca tcctgaaaat cgcaatgccg taattatggc ggatttggca
      721 ggagacattt cgccattact ggagctggat atcgatacat tgcgcgagcg tttatatacc
      781 gcaaaagccg atcttggcga taacgccgcc gttccggtta agctggtgca tatcaataaa
      841 tgtccggtac tggcccaggc gaatacgcta cgcccggaag atgccgaccg actgggaatt
      901 aatcgtcagc attgcctcga taacctaaaa attctgcgtg aaaatccgca agtgcgtgaa
      961 aaagtggtag cgatattcgc ggaagccgaa ccgtttacac cttcagataa tgtggatgca
     1021 cagctttata atggcttttt cagtgacgcc gatcgtgcag caatgaaaat tgtgctggaa
     1081 accgagccgc gtaatttacc ggcactggat atcacttttg ttgataaacg aattgaaaag
     1141 ttgttgttca attatcgggc acgcaacttc ccggggacgc tggattatgc cgagcagcaa
     1201 cgctggcagg agcaccgcca ccaggtcttc acgccagagt ttttgcaggg ttatgctgat
     1261 gaattgcaga tgctggtaca acaatatgcc gatgacaaag agaaagtggc gctgttaaaa
     1321 gcactttggc agtacgcgga agagatcgtc taatttgaag ccgccgccgc tggtacggcg
     1381 gaggacaatg ttgagtcagg ctttttgaac ggtgatgctc cacgctgcat cgccaatctg
     1441 ctgataatcg gtgatggcat gtccttcttc tgccgcccac tgtgggattg cttcagttgc
     1501 ctgggtacaa tcaaactcaa ttaccaactc atcgccgctg gccatttctg ccagtgctgc
     1561 ttttgcttct atgagtggaa acggacagac ctgcgtcacc acatccagct ttttaatcgc
     1621 catctttaca ccttacttaa tttgccgcag cagttgccag tcgcgcctta cgctgcggtc
     1681 gaacatacac aagccaggac gcagtccaga ctccgagaat cataaataca aggccaatcc
     1741 agccctgcca ggtcatcatt gcagtcataa ccagtccatt accgatagag caaccacctg
     1801 caatactggc accgaagccc atcagtacac cacctaaccc gctacgtaat gttgtttgtg
     1861 catcagctgc gcgaacgcgg aactcacggc tcgctttggc tgcaataaat gcccccacga
     1921 agatccctaa caccaggaaa acgccccagt taatgtattt cacgtcaccc gcgaccagaa
     1981 attgcagaat attggccgtt ggggaagtga ctcctaaccc aaacatgcgt ccggttgctt
     2041 cactcagggg ccaggctaaa agcgcaatca aaccgatgag tacagcagtg acaaagggat
     2101 gccagcgttt ttcaaacaga atatgagcga tcccggttcg gcgcggcggt aaggtcgcga
     2161 ctttgagttt tggcttcttc aactctttca tcaccaccca gagcgttatc accagcaaaa
     2221 cggtaaccaa cggccacaca gacaaattaa aagtctcagc aatagagtta tgttcagtag
     2281 tgtagtgctg caaggtttga tttaaaccac tggcgtgtgg agaacgcatc accgcactca
     2341 tcaccatata agtgaaaagc gcgatccaac tgccgatcaa tccctcaccc gcgcgatacc
     2401 aggtcccggt ggcacatcca cccgccagaa caattcccag cccgaaaaga tacccaccta
     2461 taacagtacc aagccacggg aacgccccgg cttcgtaagt cagtaggccc gccagaatta
     2521 acgcaaaaac ccccacgctt tgtgccgaaa ttgcaatcag caaggcgtaa aacatgcgat
     2581 tatttttcgc gatatacata tcgcgaaaac caccagtcag gcaaaaacgc ccacgctgca
     2641 tgacaaatcc cagcagagca ccacaaatta gcccgcttaa tatcattgaa aacataaatt
     2701 aattaaccag atgaatgtta atggggaaat ttttcatgac tggtggaatg cagaccaata
     2761 accaaattct ctaaattaga acaaatggtt attaatgagg atttcgataa aaaagaataa
     2821 aaaaaccgga gccaaatgtt cattcgactc cggtttatta ttagaaaaga tgattatgct
     2881 acgtcttcgt actgcggaac cggattacgg aagctcttgg tgacgcaagc caggtaaatc
     2941 agaccgatag cagcccagat cagacccaga accattgagc tttcttccag gttaacccac
     3001 agcgcaccaa cggtcagcgc accacacatc ggcaggaaca gatactggaa gtgatctttc
     3061 agcgtcttgt tacgcttctc acggatccag aactgcgaga tgaccgacag gttaacaaag
     3121 gtgaacgcca ccagcgcacc aaagttaatc agcgccgttg ccattaccag gtcgaagttg
     3181 attgccagca gggcaatcgc gccaaccagg atgatgttca tcgccggagt acgccatttc
     3241 gggtgtacat aaccgaagaa gcttttcggg aacacgccgt cacgacccat tacgtacatc
     3301 agacgcgcta cgcctgcgtg cgccgccata ccggacgcca gtacggtaat ggtggagaag
     3361 atcagcgcgc caacctggaa cgctttaccc gcaacgtaca gcatgatttc aggctgtgac
     3421 gcatccggat ctttaaagcg agagatatcc gggaagtaca gctgcaggaa gtaagttgca
     3481 aagatgaaga tcatgccgcc aatcagcgcg gtcaggaaaa tcgcacgcgg gatcacgcgc
     3541 tctgcatctt tggtttcttc cgacagattg ctgatgccgt caaagccggt aaaggagaag
     3601 cacaggattg tcgccccggt aatcatcggg ataacatgcg catcgccaga ccagaacgga
     3661 cgcgtgctcg ccagcgtacc agcgccttca ccttcaaata cgccataaac aaccatgccc
     3721 agaatcaccg cgatcagcac tacctgcaac acgacgatta cagtgttgaa gttcgctacg
     3781 gatttcagac tacgcaggtt aaaggcggtc ataaaggcca ccagcgccac cacaaacatc
     3841 cacgatggaa tggaaggcac cagagcttca aaatagattt tcgccagcag aatgttgatc
     3901 atcggcgcga acagatagtc gagcagagaa gaccaaccca ccataaagcc gacagtcggg
     3961 ctaatggatt tctgggcgta agtgtatgca gagccagcag aaggatagcg gcgaaccagc
     4021 ttcccgtagc tcagagccgt aaacaggatc gcaatcaatg cgaacgcata ggctgtcggc
     4081 acatgaccat ccgtgaggcc tgaaacgata ccaaatgtat caaacagcgt catcggctgc
     4141 atataggcta gacccatcat tacaaccgga actaacgtaa gcgttttacg caattccacg
     4201 cgagaggtgt ttggagtaac gttatgcgac atggttattc tcctttacgg tgataaccgt
     4261 cgcgtaagca aaaaatttgc cccatttttt tggattcctc agcgacaaca actgtcgatt
     4321 tttagtaaat atctatccgg tacgaagccc ggcctcttgg tatgaatgat tggtttgaag
     4381 caaaaaataa ccgacgctaa ttaaacgtcg gtttttagtc attttttgac agcggcgcat
     4441 tgtgcctaaa tgggggggaa atgacaagag aatgagaggc ttgtcataat aatttttctt
     4501 taaatggctg attttccgtc atcagatttt gcataaacac cggcgatcgc actatttgct
     4561 aaaatttcat ctcgccacca gccgctggca agccctgcgg tctgttcatt ccagccaatc
     4621 cagacaggtt caaagagagt ttgggcgacc actttcttct cgattaacgc gccactatcg
     4681 agaaaacgtt gcgccagata ccgtggtaaa tagccgcagc caaggccgct aatttgcagc
     4741 tccagtttgg ttttaaaatc gaaaacggta atggcttctt gttcgtcgag aagttgtgag
     4801 gccgtagaag cgcctgcctg cgcagtgtcc cccaccacaa tcgcccgata tcgtttaatg
     4861 atacgacggt ttaacggttc ttcttcctgg gctaacggat gatgaggcgc gaccgcaaag
     4921 acttgttcaa gatcgcccaa ccgcgagaaa ccaaactcac tggaagatgg tggctcatgc
     4981 atcgcgccaa cgataatatc cgctcgcccc tgagtcaggg catcccagga accaccgagt
     5041 acgccattga taaacttcag gcgcgtcacg ctatgatgtt gataaaaagc ttcaatgaga
     5101 ggcgcaagca gagaaaaagg aaacgtgtcg tccacgccaa tcaccagctc gttttcccag
     5161 ccttcatgca gtttgatcgc ctgcttttcc agttctcgca cggtatgcag aacttcccgc
     5221 cctttctcta ataacatttt tccggtgcgg gtgaatttag cgcggtgtcc gctacgatca
     5281 agcaattgaa tatttaggtc gctttccagc ttgtgaacgg tgtaactcag agcggacggc
     5341 gtcttgtaga gtttggctga cgccgccgca aaactgccct ctttttctat ggcatcgagg
     5401 atcatcaaca catccagcag tggtttcata cacgccccct tgcagtgcat gcggcgtcct
     5461 gctggcgtga tcgtttactc cagcggcatt accagcggat cgggatactg gtattcaaat
     5521 cccagttcat tacaaatccg actgccatca ataatcttac ctttgccgct gtccagacta
     5581 tttctgaact gcggaggttg cagccccagt aaacgggcca tctgcggata gaaaacatta
     5641 cgcgcagggt gagctggcgc acatatatta tagatgtgtc cgcctttagg tgcctgtaac
     5701 aacagagtga tagcgccaat aacatcttct aaatggacta aattaacacc atgttcacca
     5761 tcaggggcgg tttttccggc aaagaagcgt cccggatgac gtcccggtcc gaccaggccc
     5821 gcaagacgca gaatatcgac cgaagtaccg ggtaaattgt gcagccagtc ttcgagttct
     5881 tctaatactc gtccactgtt ggttaccgga ttacgcgggg tagtttcttt aaccgtgcct
     5941 tgcgcgtcgc catagacaga tgttgagctg gtaaaaataa ttcggggaat acgatgggcc
     6001 agcgcgctat ccactaactc ttgtaccgct tgtaaataga actcatcgcc ggggccgcta
     6061 cgacgtgccg gaagcgtaat gaccagcgca tcggcatcca taagggcatc cagatcgtca
     6121 gaatcgcaaa ctaactcagg ctccatgcga agcagatagc tatcaatgcc actcattcgg
     6181 gccgcttcga caccatcttg tgtggtttta ctcccggtga cttgccagcc tcgcgctgaa
     6241 agtgacatcg ccagcggcat acctaaccac cctaacccga caattgcgac ctttttcatc
     6301 cgttatctcc tgatacacct tagatctata aggctacgct agcgtatcaa aactgacaat
     6361 tcattctatg aatgaatctg ttcaataatg ataacgacat gctgcaatga gcagtgaatc
     6421 atcagtaacc gcgtatacca gacggtgctc ctctgtaatg cgtcgggacc agaaacctga
     6481 caaattatgt ttcaggggtt ctggtttccc cttaccttca aatggtgttc tgcgggtatc
     6541 tttgataagt tcattgatct ttttaacaat tcgcttatct gtttcctgcc agtacagata
     6601 atcatcccat gattcctcag accagat
//
LOCUS       DAJTXY010000170         6601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTXY010000170 DAJTXY010000000
VERSION     DAJTXY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.172"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            277..426
                     /locus_tag="OO566_003799"
     CDS             277..426
                     /locus_tag="OO566_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606893.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            627..2210
                     /locus_tag="OO566_003800"
     CDS             627..2210
                     /locus_tag="OO566_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6606894.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2662..4515)
                     /gene="asmA"
                     /locus_tag="OO566_003801"
     CDS             complement(2662..4515)
                     /gene="asmA"
                     /locus_tag="OO566_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6606895.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4537..5118)
                     /gene="dcd"
                     /locus_tag="OO566_003802"
     CDS             complement(4537..5118)
                     /gene="dcd"
                     /locus_tag="OO566_003802"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6606896.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5210..5851)
                     /gene="udk"
                     /locus_tag="OO566_003803"
     CDS             complement(5210..5851)
                     /gene="udk"
                     /locus_tag="OO566_003803"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6606897.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            6169..>6601
                     /locus_tag="OO566_003804"
     CDS             6169..>6601
                     /locus_tag="OO566_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCR6606898.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGAPIK"
BASE COUNT         1545 a         1718 c         1659 g         1679 t
ORIGIN      
        1 ctgtgcacta ttaatagcaa tgtcgccatg cacatttacc ttgcagttaa ttgaataaaa
       61 atttaactgg catcagtcct aaaaaaattg atttcatccg caggctattg acagaataat
      121 tcagactggt ctttcaggca tccagacacg ctaccgcccc tggcttttta gctaccaata
      181 cactgattga gcttaatttt tcaaaccctc tctgcataca atcgttgatg agaaagggtt
      241 attgcggaaa ttaacttccg aatataaggt gacattatgg taattgaata ttggctcgcc
      301 aataatgcag gaggaagtgt tacagctaac ggaatagcag gcaaggtaac aattcggcaa
      361 ttggctattt ttaagaatta tattaagtgt cattcaatat ggtttttagg agtttcttta
      421 ggttgacaat atttaatatt gcgtctccac atgcgatatt tcttaaataa tgttttatta
      481 ttaccgcttt taattcaggg ataatgtgag gttattaccc tcaaataata tgagataaat
      541 agtgctgcaa cattgcattt ttgccccgat ttatccatga tcgaattgtg acatttgtca
      601 tacaacgaat aggttttgta cttactatgg aatggattgc cgatccgtct atctgggccg
      661 ggttaatcac gctgattgtg atcgaactgg tcctcggcat tgataacctg gtctttattg
      721 ccatcctcgc cgaaaaacta ccgccgaagc agcgcgaccg cgcacgggtt accgggctgc
      781 tgctggcgat gttaatgcgc ctgttactgc tagcgtcaat ctcctggctg gtcaccctga
      841 ctcaaccgct gttcagcttc cgctcgttta cctttagcgc ccgcgactta atcatgctgt
      901 ttggtggttt cttcctgttg ttcaaagcca cgatggagct gaacgaacgg ctggaaggga
      961 aagacagcaa taatcccaca caacgcaaag gtgcgaagtt ctggggcgtg gtgacgcaaa
     1021 ttgtggtact ggacgccatt ttctcactcg actcagtgat taccgccgtc gggatggtcg
     1081 accatttact ggtcatgatg gccgccgtgg ttatcgctat cagcctgatg ttgatggcca
     1141 gcaagccgtt aacgcaattt gttaacagtc acccgaccat cgttattctc tgcttaagct
     1201 tcctgttgat gattggcttt agcctggtgg cagaaggttt cggcttcgtc attccgaaag
     1261 gctacctgta cgctgccatt ggtttctcgg tgatgatcga ggcactcaat cagttggcta
     1321 tctttaaccg gcgacgtttt ctttccgcta atcagacgct acgccagcgg actaccgaag
     1381 cggtaatgcg cctgcttagc gggcaaaaag aagatgcgga actggacgcc gaaaccgcgt
     1441 ccatgctggt ggatcatgga aaccagcaga tctttaatcc gcaggaacgg cggatgattg
     1501 agcgggtact taatcttaac cagcgtaccg tcagcagcat tatgacgtcg cgccacgata
     1561 ttgagcatat cgatctcaac gcgccggaag aagaaatccg ccagttgctg gagcgaaatc
     1621 agcatacgcg gctggtggtt accgacggtg atgacgcaga agatttgctc ggtgttgttc
     1681 acgttatcga cctgctacaa cagtcactgc gcggcgaacc gctcaacctg cgggtgttga
     1741 ttcgccagcc gctggtgttc ccggaaacct tgccgttgtt accagccctg gagcagttcc
     1801 gtaatgcccg cacgcacttt gcttttgtgg tggatgagtt tggctcggtg gaagggattg
     1861 tgacattaag tgacgtcact gaaaccattg ccggtaactt accgaacgaa gtggaagaga
     1921 tcgacgcccg ccatgatatt cagaagaatg cggacggttc ctggacggcg aatggtcata
     1981 tgccgctgga ggatctggtg caatatgtgc cgctgccgct ggatgaaaaa cgtgaatatc
     2041 acaccattgc cgggctgttg atggagtatt tgcagcgtat tccaaaacct ggcgaagaag
     2101 ttcaggtggg ggattatttg cttaaaacgt tgcaggtgga aagccatcgc gtgcagaagg
     2161 tgcagattat accgctgcgt aaggatggcg agatggagta cgaggtgtga tggtatcggc
     2221 cattttaccc aatgctgcgt aacgccttat aacttcagca atgaagcgtt gtctgtggta
     2281 aacggacatc acagcggggg tgtgggtccg gctgtaaatc gccgaatcgt ttcctgtagg
     2341 ctggggcagc ccacatggat gtgggctgag ggcgcgtttt acagggatgt tacctcgcgc
     2401 ccgtacccga tagccgaaag gaataagatg agggaaccgc gccagcggcg atttaccgcc
     2461 gggagcccgg gttgccaggg tggtggcgat tgagccaccc tggcacgttc accggcttga
     2521 gcgttccata gtagcgagga acatcaagtg aacggaacag ccttcgatct tacatattca
     2581 gaatataata gtgccgccgc gaacgcctga tgcgacgctt tacgcgtctt atcaggccta
     2641 caggacaggt cacctggtaa cctacatctt ctccagcaac ttcttcacat ctttgccatt
     2701 gcgggaatct ttattccgct cggcccagtc attcaggcga cgtttcgctt cgtcctgtag
     2761 atgtttgcgc agtaactgat ccacttgcag gctgtaattg agttgctgcc agttgccata
     2821 aacccgcagc ggtactggcg tttctttgag gaaatcaatc agtttgcttt ccccgttcca
     2881 gccacccacg acccgaatat caaactgggt gtcgcaggtt tgatctgcca gattcaacat
     2941 gccttcccct gtcagcgcca gcacaggcga ttgaccttgc atgtcgttta acgtcacgac
     3001 gccatccttc aacgtcaaat cggtggtaaa gcggtcaagc cgcgtcacgt tatcgaagtt
     3061 ttcagcggcc ttcacatcac cgccattacg ctctaccgct tgctgaatca tctgctggaa
     3121 gttcatccct tccatacgtg tgtcggtcat ctcaacatgc gcctgccctt gccagttgtg
     3181 gcggaatgcg tcggcatcta tgtcagcacc ggagaaatca ccagccagtg acatttttcc
     3241 ggtcaacgaa atcggatagt taaacgcctt cagaatggta ccaatctcaa cgttctccag
     3301 ccgtggctgg aagtttatcc gcggatttat tgatgtcgcg tccagcgtgc ccggcagtga
     3361 aacctttcca ccgttaagct tgccctgcag ttgagtaatt tccagcaaac ccgacttgtt
     3421 ggtcatttgc gtggcaacat ctgtaaaatt cattccgcgc cagcgcacgt tactggcctg
     3481 caacaaaata tcagctgtaa agccttgcag tccctgatag gccggttcat caatacgcga
     3541 agaaatgacc gggcgcggca acgttgattg gctctgcccc tgctgcgcgg caccgttttc
     3601 accattcgcg gtttcattaa gcgggatgag gttgtcaaga ttcagttgcg ggaattgcag
     3661 tctcagctgc cattccggtt tctctgtcag cgtgacctgt gcttgcccgc tcagcgtact
     3721 gtcattggcg gtcaaactaa tttggttaaa tgaaaggcgt ttatgtgact cctgccactg
     3781 ggactggaaa ctcccctgtc cctgaatacc ttgttttggt aaatcggcac cctgcaactg
     3841 ccagttaatt tgttcaatag ccgctgttaa atcatgcgga taatcagaag catctaccgt
     3901 accgttaagg gatatcgtga gatcgcgctg atcgcgatta acccgcccgg agaactcaaa
     3961 tgagccacga tgttggggat cttgttccat ttgcaggcgg atattgcgga ttgtcacctg
     4021 ctcgtcatct tcatgctgga acaccagaac gctgtccgcc accttaagac tggatatatc
     4081 aaacgaccat ccgcgatcgt ctgacagatc cggcaaggta ttgtcgcgcg gtgcaaccgg
     4141 agcgtcttca ctgcgcaccg cttccgtctg cggcgtcagt tggatcactg ccccttttag
     4201 catcacctgc ttaacgctca gttgatgact cagtagtggt aaaagcgcca cgtccagacg
     4261 catgttgtcg gcgcgaacca gtggctggct tgcgccctgg gcggtgagag acattcgccc
     4321 ggagaggata ctaagctgcg gccagacgtg ccaacgcagt ggcccgtcga gctgcaattg
     4381 ataaccgcta cgtgcagcaa cttgcttgac catatagtcg cggaaatcat tcggattcac
     4441 cagcaacact aacgcagata acccggccac cagcacgacc aggagtatca tcagcgtcgt
     4501 cagaaatcgt ctcatggtat cctcaatggg cctgaattag tctttatcga ttcggctggc
     4561 taccgcgccc tgctggttgc gatatttcgc atcttcacgg cggttgtaag gtcgcgccgc
     4621 cgggccggaa agcggctcaa agctcagcgc accaattaac atgcccggac gcagcgccag
     4681 cggcagctta ccggagttgt agaactccag cacaatgcaa ccagaccagc ccggatcgat
     4741 acggtgcgcg gtgacgtgca ccatcagccc cagacgcgcc aaagaggaac gcccgtccag
     4801 ccagcccacc agatcggctg gcagcgtcac cgactccagc gtcaccgcca gcgccagctc
     4861 tcctgggtga agatagaacg cctcgccctc gtcgagaacg atctcatcgc tcatcacgcg
     4921 gtcaagcgcg gcgctcactt catctttggg accgctcaga tcgataaacg ctgccgtgtg
     4981 accacggaag gtacgaaatt tattgcccag gcgtacatcc accgtcgcgc cgttaatacg
     5041 ctccactggc ggacgtgggt tgatcgacaa acggccttca tcaagccagg cttcaatatc
     5101 tcggtcacac agacgcatgg cattttctcc tttcgcgcat cactccctta acgccaatta
     5161 cgtcaagggc ataccagctt atcactgaac ggtacacaat ttatcaagct tattcaaaga
     5221 actgacttat tttcgctttc aatatatcga tcgcgatgcg gtttttcccg ccgcgcggca
     5281 cgataatgtc cgcatattgt ttagaaggct caatgaattg caggaacatc gggcgcacgg
     5341 ttttttgata ttgcgccatc acggaatcca ttgaacgccc acgctcgtta acgtcacgct
     5401 tgatgcggcg catcaggcag atatccagcg gggtatcaac gaaaatggag aagttaagtt
     5461 cgtcacgcaa acgcgcatcc gtcagcaaca aaatgccttc gagaatgatg accttcttcg
     5521 gctcaacctt caccgtttct ttcatacgcg tatgttcaac atagctgtaa accggcaggt
     5581 caattgccga gccgcgtttc aacgcttgta aatgctcaag caacaggctg tgatccatcg
     5641 cgctgggatg gtcgtagttg gtcttaacgc gttcttccat cgacagatgg ctttgatctt
     5701 tgtaatagca gtcttcggga attacgccga tgtgttcatc accgacttgc tcacgtaatt
     5761 cacgataaag ggtactggca ataagactct tgccggaagc cgatgcgcca gcgataccga
     5821 taatgacgca ctgatgagac tgatcagtca tatatttagc gacctgatta acctggatgt
     5881 taggaagggg gcgacgaagc gccaaacgcg gcaattatag ggatttcatc cgtctgatac
     5941 cagtcgaata gcgttgccgc gcgctcagag ttaattgttg acaaagaatt cccggtggca
     6001 aattacgttg atcagtttta tgtaaggtaa aaaatgttat acgcagttgc gcaaattatc
     6061 cgcctttacg tcactttatg agcaattcgc atataaaatg taaaactttt gtactagcat
     6121 aaacacagaa acgaatactg gcgacctggc cttgcggata aagcggtaat aagcaaacaa
     6181 tcacagcatg tattaattgc cctgccccac ccgctgcttc acctggtcag tttaggttta
     6241 gtctcgttta tctttactct tgtctcgctt gagctttcgc agtttggcac ccaactcgcc
     6301 ccactgtggt tcccgacgtc catcatgatg gtggcgtttt atcgccatgc cgggcgcatg
     6361 tggccgggaa ttgcgctgag ctgttcgctg ggaaatatcg ccgcatccat cctgcttttt
     6421 tccaccagct cgctgaacat gacctggacg accatcaata ttgttgaagc cgtggtcggg
     6481 gcagtgctgc tacgtaaatt gctgccgtgg tataacccct tgcaaaatct ggctgactgg
     6541 ctgcgtctgg cattcggcag cgccattgtt ccgcctctgt tggggggtgc ccctattaag
     6601 t
//
LOCUS       DAJTXY010000171         6577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTXY010000171 DAJTXY010000000
VERSION     DAJTXY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.173"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1117..2064
                     /gene="icsP"
                     /locus_tag="OO566_003805"
     CDS             1117..2064
                     /gene="icsP"
                     /locus_tag="OO566_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6606899.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2626..2998)
                     /locus_tag="OO566_003806"
                     /pseudo
     CDS             complement(2626..2998)
                     /locus_tag="OO566_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3242..3361
                     /locus_tag="OO566_003807"
                     /pseudo
     CDS             3242..3361
                     /locus_tag="OO566_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            3611..4477
                     /gene="ospB"
                     /locus_tag="OO566_003808"
     CDS             3611..4477
                     /gene="ospB"
                     /locus_tag="OO566_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6606900.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            4806..5546
                     /gene="phoN2"
                     /locus_tag="OO566_003809"
     CDS             4806..5546
                     /gene="phoN2"
                     /locus_tag="OO566_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6606901.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(5899..>6577)
                     /gene="ospC4"
                     /locus_tag="OO566_003810"
     CDS             complement(5899..>6577)
                     /gene="ospC4"
                     /locus_tag="OO566_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6606902.1"
                     /translation="VSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFRE
                     FCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEIN
                     NKVTDKKMAHQALAYSLGDKKADIALYLLSKFNFTKQDIAEMEKMNNNIYCNLYDVEY
                     LLSKDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAV
                     SGKRFER"
BASE COUNT         2077 a         1237 c         1252 g         2011 t
ORIGIN      
        1 ggaataagga caatagctta tccaaactgc caagcctctt tattataagt aagatctggc
       61 actggaatat tctcaaaagg actaacatca tcaccacgac cttcaaccaa ccattttatt
      121 gcatttaaat cccagaatag ttcagtagtg gtcaatttgg gaaacgcaca atattgtacg
      181 gttacgttat cgcaatagag tgaactcccg ccctatacgg cgttttcaag gattagtcct
      241 taatcggaca accacattaa agcataataa ataattaagc tttaataata tactatagat
      301 acagttctat tataaaaaat actattttag gtgatgtatt aaattagtag aatatttatt
      361 cttttataaa agcaccaatg aattgtccaa gtaaacatga ggtaaaatgt gtagaggata
      421 actgagtttg gtcactttaa ctgtattagt cgcagtctga ctctcgactt taaaaggatg
      481 ggaattacct gtactgatag tgagaaagct ttccattgaa ataagtcaaa taaaagttga
      541 tggcttcagt gctaaagtga ggtgcccgct agccgtattc actcgaatcg gaattcaatg
      601 tattgcttct gccatttcca taatatttca cgggaaaaac aaaatctgaa aaactatact
      661 taagtgttgt attaagtcag ttgagataca aatacatatt tatcagcaca cgttacgtca
      721 aatacaggag gttgtcatgt tggtactgaa aggcacgttg gctattgcag ccgtgatgac
      781 aatttattgt cttatcgttg ttcttatgga acgcttttct gattgatttc atattggcga
      841 gagaacaaga aggtgagtag aattgtcgcg gaatcctgaa actatcagcc tcaggtatga
      901 acaccaaaag gtaggtagag aaatctatgg cccccgttac tttattcgca caatgaggta
      961 tttttttatt tttacaaaag ttcaataaat attataataa aagatatgtt cttggtaaca
     1021 taaacaaata tttaattaaa ataatgcttg gcattgacgt ttatcttata aagtaagaag
     1081 atcatggaca tttcaaccaa aaaagtagag ttctcgatga aatcaaaatt ccttgtactt
     1141 gcactttgtg tacctgcgat ctttactaca catgctacca ctaactatcc acttttcata
     1201 ccggacaaca tcagtacgga tattagtcta ggatctctga gtggcaaaac aaaagaacgc
     1261 gtttatcatc ccaaggaagg ggggagaaaa attagtcaac tggactggaa atacagtaat
     1321 gcgactattg ttagaggtgg catcgattgg aagctaattc caaaagtgtc tttcggagtt
     1381 tccggttgga ctactttagg taaccagaaa gcaagcatgg ttgataaaga ctggaacaat
     1441 tccaataccc ctcaggtatg gaccgaccag agctggcatc ccaatacgca tctccgtgat
     1501 gctaacgaat tcgagctgaa tcttaaaggt tggttattaa ataatttgga ttatcgactt
     1561 ggactaatag caggttacca agagagtcgt tacagtttta atgcaatggg agggagttat
     1621 atttatagtg aaaacggggg gaacaggaat aaaaaagggg cacatcctag tggtgaaaga
     1681 acaataggtt acaaacagct cttcaaaata ccttatattg gattaactgc taattaccgc
     1741 catgagaatt ttgagtttgg agcagaactg aaatatagtg gttgggttct ttcatctgat
     1801 acagataaac actatcagac tgagacaatt tttaaagatg aaataaaaaa ccaaaattac
     1861 tgctctgttg ctgcgaatat tggatactat gtcaccccca gtgcaaaatt ttatatagaa
     1921 ggctccagaa attacatttc taataaaaaa ggtgatacat ctctttatga gcaaagtacc
     1981 aatatatctg gcaccattaa aaatagtgca agtattgaat atattggttt tctcacttcc
     2041 gcaggtataa agtatatttt ttgaattggt tcaccgagtt gattacgttt tcatggctaa
     2101 aaattgccgc ccattgtgag tcatattcgc cctgactttc cagaaccata cgaactgccc
     2161 gttgacggac ttcgggggaa aaacgtgtat ttttagtcat tctgtttacc tctttctcag
     2221 ggcgtttagt ctccaggatt tccggggcgg ttcaaaaaac tcttcgttga ttactgtgga
     2281 cttactgttg gcattaccga ccctgagacc ggagaaataa gaaccgctta gatcatcgtg
     2341 gctgttctcg gggtatcaag ttacacatgg gcagaggcta cccaaggtct actattatcg
     2401 cctgaaagcg ctgatggaac agtgctatca gggacatgct gatgaaaata cctaaataat
     2461 cagccagcac tctatctttc caaatccaca gcatagcaaa gagagcaaaa gagcctgtaa
     2521 attcagaaat taaggaaacg cctcttggtg cacataatct atcttatgtt aaatgaaatc
     2581 taatttcttg attatatgct ggatcgttcg tgggtggctt tgcccctata tttccagaca
     2641 cctgttatca ttggcccgct gccgcagata ttcccgtggc gagcgataat ccagcgcact
     2701 atgcggatgc cattcgttat aatgctcgaa tgcctctgca aggttctttg ctgccgttaa
     2761 cccgtcaggt ttgggcatga tactgatgta gtcacacttt attgttttca cgaagctctt
     2821 tgctattctg ttactctccg gactccgcac cgccgtgttc ttcggttcaa caaagtccca
     2881 acatccgggt gaactggcgt gtttcattag cccggtagca tgaaccatta tccgtcagtc
     2941 actccactgg agacgacgga agatcgttgc cgaagcggtg ttccaccgct cccagcatgt
     3001 cgccaatggg agagcatcta cggtctgatt gaactgttta ttcaaattgt ccaccgtctg
     3061 tcagtacgct ctgaacgtcg gctggttaaa acactgctcg ctgattttca gagggttcac
     3121 ggtaaaacag cacttttatt ccggattgcg gaagcggctc tgaataatcc ggatgggctg
     3181 gttaaagagg ttgtgtacca tctgcatatt gttgaaccag atcgcagcgg taagagaagt
     3241 tcatcatact tggcgcagtt acgcgatgtt tctgctagag gtgatgccgt aaagaacggc
     3301 agaacattac cggaacaaga tagcggtcta cctgcactgg tatcagaccc ggggcttccc
     3361 cggatgatct ccacggtact ttaggtactg tgtctatatt atgtaggtag gatcaattaa
     3421 ctttgtagtt gaaattcttt cattgaaata atgaataaaa aagacaaacc gcattaaaca
     3481 atgccagaat ataattgcat ttttgttccg tttttttaaa tcatagtata caaagaagct
     3541 tgcatactat atgaaaatat aacacccttt actatgatgt ttataaaaca atatatggag
     3601 tcatgtaggt atgaatttag atggtgttag accatactgt agaatagtca ataaaaagaa
     3661 tgaaagcata tcagatattg catttgcaca tataataaaa agggtaaaaa attcatcatg
     3721 tactcaccca aaagcagcat tggttttttt aggagagaaa ggtttttgtg atagcaatga
     3781 tgttctatct attatgggac aacaaatacc aagagtattt aagaacaaga tgttatatga
     3841 ttatgttttt aaaaatgaaa aaagtaaaaa tgattttcta aaaatggctg aatcatggct
     3901 accacagagt gaaccaatag taataaataa tgatgatgac gcattgaatg ctgctgctta
     3961 tttttctgta aaaaaagcga aaataaaaac agtaaacgat actgatttta aagagtataa
     4021 taaggtttat attcttgggc acggtagtcc tggttctcat caattaggcc ttggttcgga
     4081 acttattgat gtacaaacaa tcatttcaag aatgaaagac tgtggtattc taaatgtgaa
     4141 agatatccgt tttacttcat gcggctccgc tgataaagtg gctcctaaaa attttaacaa
     4201 tgcccctgct gaaagtcttt cttgtatcct taactctctg ccttttttta aggaaaaaga
     4261 atctttgcta gagcagataa aaaaacacct tgaaaacgat gagtcattga gtgatggtct
     4321 aaaaatatcc ggctatcatg gatatggagt tcactatggt caagagcttt ttccctactc
     4381 acattatcgt tcaacttcaa ttcctgctga tccggagcat acagtaaaaa gaagctctca
     4441 gaaaaagact tttattatta ataaagaact ggattagtat aaaattttta acctatagtt
     4501 aattgtttgc atttaagatg aagttatttt atctttagta gaaaagtgct tccctatgag
     4561 ggaaaatcac aaatcatcat aatacagaga caaaacgata cgaacaaata gataaaaaac
     4621 atcattcttt taccacatta tttttcgtga atatgaaaaa taatgttatt actttaatat
     4681 aagactattt tttgtttttc catcactctg ttcaaatttt tccgcatgac ttgtgttttt
     4741 tgtaacacag ctcgtttttt acagctgacc aaaatcatca attaattatg ctaaggaaat
     4801 aaattatgaa aaccaaaaac tttcttcttt tttgtattgc tacaaatatg atttttatcc
     4861 cctcagcaaa tgctctgaag gcagaaggtt ttctcactca acaaacttca ccagacagtt
     4921 tgtcaatact tccgccgcct ccggcagagg attcagtagt atttcaggct gacaaagctc
     4981 attatgaatt cggccgctcg ctccgggatg ctaatcgtgt acgtctcgct agcgaagatg
     5041 catactacga gaattttggt cttgcatttt cagatgctta tggcatggat atttcaaggg
     5101 aaaatacccc aatcttatat cagttgttaa cacaagtact acaggatagc catgattacg
     5161 ccgtgcgtaa cgccaaagaa tattataaaa gagttcgtcc attcgttatt tataaagacg
     5221 caacctgtac acctgataaa gatgagaaaa tggctatcac tggctcttat ccctctggtc
     5281 atgcatcctt tggttgggca gtagcactga tacttgcgga gattaatcct caacgtaaag
     5341 cggaaatact tcgacgtgga tatgagtttg gagaaagtcg ggtcatctgc ggtgcgcatt
     5401 ggcaaagcga tgtagaggct gggcgtttaa tgggagcatc ggttgttgca gtacttcata
     5461 atacacctga atttaccaaa agccttagcg aagccaaaaa agagtttgaa gaattaaata
     5521 ctcctaccaa tgaactgacc ccataaagct ggacagcctg tatcaggcta tggagggccc
     5581 atagacaaat ctaccctata tgagcatagg aggagtctat gggcacacca cgttttactc
     5641 ctgaatttaa gggattactg gaaaggctgg gacatatccc tccggcagaa gcagaaaaag
     5701 cttattatgc tgccatcgga aacgatgatc tggcaacctg agttcacaga taaaacattc
     5761 tctaggaaac tcggggcggt tccgttcacc acatgcaatg tggtgttgca ggggaacggt
     5821 ctgcaccatc ccctatatcg atcaatataa caaaaatgac aacttcagat tcagggcaca
     5881 acctaaatat attttaggtc acctctcaaa tcgtttgcct gataccgctc cattttttaa
     5941 taaaaaatca atcatttttg aatctttgct tttcattgca ttgtcaagca tagtgtcccc
     6001 actatttgct ttttgaaatt ttttgtttac atcaaccaat ccattattga taaaatattc
     6061 tagcacttta tagttggcac catctttact aagtaagtat tcaacatcat acagattaca
     6121 atatatattg ttgttcattt tctccatctc tgcgatatct tgttttgtaa aattaaattt
     6181 agatagtaaa taaagtgcta tatcagcttt tttatcgcct aaagaataag caagggcctg
     6241 atgagccatt tttttatcag tcactttatt gttaatctct tcataattag atgctttaat
     6301 cgcatcctcc aaagatatgt ctctgagttt tatttttttg aagttatctg tacttagcat
     6361 tcttggtata tgatactcaa gctgaaacac aaagtttata attctatcca gactaacagg
     6421 atcaatgttt ttgttataac aaaactctcg gaatccttga tctttactct ttcttataaa
     6481 atcaataaga tgcaacccaa ttccaagcct catatcttta gtgttaaata ttggaacatc
     6541 agttttcccc tgattaccat gcacatacct gctaact
//
LOCUS       DAJTXY010000172         6528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTXY010000172 DAJTXY010000000
VERSION     DAJTXY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.174"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..336
                     /locus_tag="OO566_003811"
     CDS             <1..336
                     /locus_tag="OO566_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6606903.1"
                     /translation="SLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKR
                     GDKNLRRLLVQCARSFMIQLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWAI
                     TTQQKEYQA"
     gene            complement(639..1409)
                     /locus_tag="OO566_003812"
     CDS             complement(639..1409)
                     /locus_tag="OO566_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606904.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1454..1888)
                     /locus_tag="OO566_003813"
                     /pseudo
     CDS             complement(1454..1888)
                     /locus_tag="OO566_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1900..2121)
                     /locus_tag="OO566_003814"
     CDS             complement(1900..2121)
                     /locus_tag="OO566_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606905.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2122..2805)
                     /locus_tag="OO566_003815"
     CDS             complement(2122..2805)
                     /locus_tag="OO566_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6606906.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2869..3180)
                     /locus_tag="OO566_003816"
     CDS             complement(2869..3180)
                     /locus_tag="OO566_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606907.1"
                     /translation="MKYDGDGRATARFFSDKGCRRVPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3177..4079)
                     /locus_tag="OO566_003817"
     CDS             complement(3177..4079)
                     /locus_tag="OO566_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6606908.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4519..5478
                     /locus_tag="OO566_003818"
     CDS             4519..5478
                     /locus_tag="OO566_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6606909.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5478..5873
                     /locus_tag="OO566_003819"
     CDS             5478..5873
                     /locus_tag="OO566_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6606910.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6056..6436
                     /locus_tag="OO566_003820"
     CDS             6056..6436
                     /locus_tag="OO566_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR6606911.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1779 a         1692 c         1587 g         1470 t
ORIGIN      
        1 agcttactat cgtcgcaact tggtgacggg aaacagtttt catgcagcag agattttgcg
       61 gcttccacag ggctcgttcc ccggcagtat agcactggag gaaaatcgac tctgttgggt
      121 atcagtaagc gaggtgataa aaatttacgc cggttactgg ttcagtgtgc ccgctcattc
      181 atgatacagc ttgaacgcca gcatggaaaa cttgctgagt gggttcggga gcaactcaac
      241 aagaagcatt cgaatgtggt tgcctgcgct ctggcaaata aactggcccg aattgcctgg
      301 gcaatcacta ctcagcaaaa agaatatcag gcctgatata tagatgctga caaaactcaa
      361 ttagcttatt gcaaacaata agatacaagt taccgtctgg ttttgcgaag attgattatt
      421 gatgacatga acggcaaatc gacctgatta aaaacctaac aggggaaaat ggctcactga
      481 agccgagggg atttttagga ttatcaggtg cggaactcat cgaggcgcgg gcatcccttg
      541 cccacagaga cgccggatag atttaagcaa gcccaccatt aatcaaaaat cagtgttgca
      601 aaaacggggg tgaccataga ttttccccgc catcgtcatc atttccggat ttttgccagg
      661 gcatccgcca tgctgcggat ttcggcccgg gcttcccgga caacttcccg cagaacacca
      721 cggtcaaacc accggcgggg cggttcatcc ggtatacgca gcacatcgca ggccagccca
      781 ctggacagga tatgctcaat acaatcgctg accagataca tttccaccgc cgaaaaacgt
      841 gcgccccggt tcaggatggc atcgagcacc acgggcaggt tttcatcgtc ttcatcgagc
      901 cagcaggggt gatcgctttc cttcacgacc aggcgaaagc ggcgcgtctg gcgggtcagc
      961 aacgtctgac cgcttgtggt cagcgccgcc cggatacgcc gcgcatcttc tgccccccgg
     1021 caactgacac tgcatactgc atcgcgcacc gcatcaagaa ctgccctgat ttcgtcttcg
     1081 gtcagccatt cacaggcata cgggaaagac acctgcgttc cgtccgccgg aagtagcggc
     1141 agcgactgcc actgactgcc ggacagaaca agttccgctc cggttccggt cagtttctga
     1201 atggcggagg ccaggtgcgg cggcagcacg ccatcgtggc ggttaacgag taacgtcgcc
     1261 agccggggcg catccagccg cacgtaaatg ctgatccagc ccggatagtt cgggttacat
     1321 tggtcaacat ccgtctcttc cggccagccc tgcgccagca gcaggttacg ggcgtgacga
     1381 caaatcagag cattcagcgt ttcattcata attcatgaac tccctgtatc gccgcccgca
     1441 ggcggcgata acatcagtcg ttttcgtgtg tgttgttttt cagcaggctg cgggcctgat
     1501 caaccatcgc ctgtcggcgg gtaacgtatt ccgggagggg gaaaccgtga tcccgggcag
     1561 cccattcacg tttccacagt gcctgttcag cgatctgagt cagggtctcc agcagttcag
     1621 cagggacggt cacctggcgg ttttcgcgca cgttgctgat aatctccatc gccgcggcgg
     1681 aagaaatacc ggactcaatc cgctagtctt ccgggatgcc ctccaggtgc gccaggacgg
     1741 tcctgatttc atgatcggac agggaaatat ccattccgtc agcgagggcc tgaatctctt
     1801 ccggggacca gataaccagc atcagcggcg tgtctcccga atactttgct gccagcgccc
     1861 ggcatagcgt ttcgcatgtt ccgtacatac tctctcccct caatctttga agcaggaatc
     1921 cagttccaga atttcggtat atgcctccat caagaggcgg tttgcgcgaa acaacgtctg
     1981 acgcaccaga cgttccggca gggtgtcagc attaaagctg taccgccgct ccatctcatt
     2041 catggttgta cgcaggtcgc acatggcatt ggccacctcc gccacttcgg cgcgaagttt
     2101 ttcgtgtccg gcatagttca tttacgcagc ctccggcata aaccagtcat cattcgcggc
     2161 cccctgctgc atggcccgct gtacggcagc cagtcgttgc tgtccggcac ggtgatactg
     2221 ctcaagcagc tcgataccga tataccggcg tccggactgg agggcggcga cgcaggttga
     2281 accgctgcct gcaaacgggt ccagcactat cgcattcggg tgtgtgaagc tctcaatcag
     2341 cggttgcagg ctggtaacgg gcttttccgt cgggtgatgg cggttgcccg aatatttcca
     2401 gcccaacacg tccggcagcg ggttttgtgg cagacgtgga cggccttttg ccaggatgta
     2461 ggcgcattcg tggcgatagc ccacatatgc ggccttcgat gtgtagtttt tggtgaagac
     2521 caggtgacca acaacgctga atcccgcatt tttccaggca gacataaagc gatcgacgcg
     2581 gttccagcca tagaagctca ccatcagcgc gtcttttttg agtacgcggt acatttcatt
     2641 acaggccggt tgcagccatt catcagtttt atcaccagca atggtacgcc cctgacggtc
     2701 acgaaaaccg acgagatatg gcggatcggt gaggatgaaa tcaatggcgt tatcaggaat
     2761 acgggccata acatcgatgc agttaccgag gacaaaacgg gacataacag atccttctct
     2821 tacagagggc acccctgtgg tgcccgttcc ccgctgcctg tgggctgttc atcgcgtccg
     2881 gtaacgggtc aggccgaccg tggaataccg cagccgtcgt ctttttgaca gcaaggttat
     2941 gccgcgaaag cggcttgaag ggcgttcatc agaaaagggc gacgggctgg cgtacactgt
     3001 caccgacgcc agccgggagc ggtaaaagcg cccgtcccgt gcccgccgga cgcgggacac
     3061 tgaccacagg cagcgtttat gccgcgccgc gtcagccggc gcagtaaaaa gaggaacacg
     3121 gcgacatcct ttgtcggaaa agaaacgggc ggttgcccgc ccgtctccgt catacttcat
     3181 ccgccataat gcgccacatt gtcgactatc cacgcaggtc cggtgacttc cggctgctca
     3241 tcgtcatctg tcggggaaga ccattcaagt tccccccaca gcacatcgac gagctctcca
     3301 cgctcataca actgccagcc acagaaatca cagccctgtt cggcatacca gtgctccagc
     3361 gtgcagctga aacggcggct taattctgca ataacgtcgc tttccggctg acaccacggc
     3421 gtgtcgaaat ccacctgaat gaagttttct ccctgactgc tgatattcag gtcatatcca
     3481 caaggccact tcacgccata ccgttccgta taccagtgat atgcagaagg aacaccgttc
     3541 agaacaccac cattaaagcc gttcacttca acatccaggc gggtcggcag aaccgtcagc
     3601 aggtcaaacg gcatggtttt ttccggcact acgttgtcac acagacggtt ccaccacaca
     3661 gatacatcct cgttacccca tatgtcacac cagtcacctc tttttgcggt gaaatgcaca
     3721 gtgatggttt ccctggcatc atccggtaat ccttcccagc gacgctggcc agtaccgctc
     3781 tgtaaccaga gttcatgcag catcaggcag ttttgttcat ccagcaacac accgttctgc
     3841 aggtgtgtca gccagcgggt gaacgcgata ttttccgtcg atacagcacc acgtccggca
     3901 gccgtcactc cggggcactg ttcagagcgg atattttcag tgatctgcag aagtccggca
     3961 cttccggcca gaaacagctg aataccttca tttgtggcgc ggcgataaag cggtgtgact
     4021 gcaccgctgg ccagtcgttt aatctcagcg atctgtgccg gttcaccagg aaaatacata
     4081 cgattcgagc accagtttgg catgggaatt ctcctctcac atgcggcctc catcaggccg
     4141 tgccgcacct gtaaccgggc gaccgcatac gaatgcaggt gcggccccga agccgcagcg
     4201 cgtggaggag gaatgacgaa acgtgaccgg gtgaggaggc cggcgacagc gacacacttg
     4261 catcggatgc agcccggtta acgtgccggc acggcctggt taagtagtat ccgtctcagt
     4321 tcgtggcgca aaatgagaaa ccaataagaa ctcaaaatga accacaacaa gaacataaag
     4381 agactctaaa agagcaacac cgcttgacat agactcaaaa tgagtcacaa taagaaacaa
     4441 taaaaaactc aaaaagatgc accgatataa aggatgctca atttgagata aacacaaact
     4501 caaaacgagg caaaaagaat gctaaaagta tcatgcgatg atggttcaac taacgtaaaa
     4561 ctggcctggc ttgaagatgg cgaggtaaga accagtctgt caggaaacag ctttaaagaa
     4621 ggctggaacc cgggactgtt taatgccggc aaagtttaca attacgtcgt ggatgaaaaa
     4681 aaatacacct atgatttggg aagcactgct gtcataggta caacacatgt cagttatcaa
     4741 tacagcacca ccaatctgct tgccatacat catgcactgc tgacatccgg ccttcagcca
     4801 caggatgtag aactgacagt aacattacct gtgaccgaat tctttgataa tgacaatcaa
     4861 ccaaatgaag aaagaataga aagaaaaaag gcaaatgttc tccgtgagat atctctgaat
     4921 aaaggggaaa catttaaaat taaaaaagtt aatgtaatgc ctgaatcttt gccagctgca
     4981 tttgaatcac taaaaaaaga taaagtaaac aaacttgaac gcagcctgat tatcgatctt
     5041 ggaggaacca ctctggattg tggtttaatt ctcggtgcat ttgaaggcat ttctgaaatc
     5101 cgtggctatt cagaaatcgg gacatcccgc atcactcata ccgtcatgaa tgcgctgacg
     5161 aaagcctcca caccatgtaa ttattttatt gctgatgagt taataaaaaa cagacacgat
     5221 aatgaatatc tccagacatt aataaatgat gtagctgaaa taaaaaacat ttctcatgta
     5281 atagacagag aagtgaaatc tctggcagaa agcatcaggc aggaaatttc aacattctca
     5341 ggaatgaacc gaatctatct cactggcgga ggagccgagc ttatttaccc tcacataaag
     5401 caatatttcc cgaatctgaa agtgaacaag gtggatgaac ctcagtttgc attggtgaaa
     5461 gcaatggtgc atgcgtaatg gaaagcagtg atccaaaaaa aagaaagaaa gttgtggcat
     5521 acctgcaccc ggcactttat ccgcaggaca acctgacaca acaaacaata gactctctcc
     5581 cagtacaaat gcggggagat ttctacagac aatcccttat ttgcggagca gctctttatt
     5641 ctgttgaccc ccgactgtta acgctaatca gcggtttttt cagtgagaaa atcactgcag
     5701 aaaatctggt gaaacttata gaacaaacaa caggatacac atccacatca attgatataa
     5761 gtgtgttaaa aaacatcata gaggcatctt cagaaaacaa atcagagagc atcacttcaa
     5821 aagatgattt cgaagaacaa accagacgca atctttctat gttaaaaaaa taataaaaga
     5881 agagcaggtt cagtcaatta cctgctcttc ttttatacat actaaaaaat cagattttta
     5941 cgaatattca gattatcata aaaaatgcaa cagacaggaa tgcaataatt ttgcattatc
     6001 tcgttgcttg taatgcaaat atgcattatt gtcagtgtgt gacaataaca gagagatgac
     6061 gatggcgacc attaacgcac gtattgatga tgatataaat aaccaagcag atgaagtgct
     6121 gaagctgatg aatatcagtc aaactcaggc tattgcagct ttttatcagt atattactga
     6181 acaaaagaaa ctgccatttg tgatcacctc aattgtaaaa acgccgcatg atttattacg
     6241 tgaaagtaca gacatgttag ctgaagctct tgcagtgatt tccaatctac aggtgtggac
     6301 tgaacagcag gatgggattg gaaaagcaaa gctaatggag tattaccgcc gcctcgacgc
     6361 actgtactgt tgtgcaaaag agaaaatagg tcttctttca gataaccggg atgctgaact
     6421 aggctgtgtc ccttaatcat ctgagttata gtaactgtct gtttacacaa catattgaac
     6481 tatggctcgc tacgaccttc ccgatgaggc atggaccatc atcaagcc
//
LOCUS       DAJTXY010000173         6491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTXY010000173 DAJTXY010000000
VERSION     DAJTXY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.175"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..368)
                     /locus_tag="OO566_003821"
     CDS             complement(<1..368)
                     /locus_tag="OO566_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6606912.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            892..1866
                     /gene="hemB"
                     /locus_tag="OO566_003822"
     CDS             892..1866
                     /gene="hemB"
                     /locus_tag="OO566_003822"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6606913.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="OO566_003823"
                     /pseudo
     CDS             complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="OO566_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2808..3647)
                     /gene="tauC"
                     /locus_tag="OO566_003824"
     CDS             complement(2808..3647)
                     /gene="tauC"
                     /locus_tag="OO566_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6606914.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAVTRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQW
                     VNV"
     gene            complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="OO566_003825"
     CDS             complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="OO566_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6606915.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4424..5350)
                     /gene="tauA"
                     /locus_tag="OO566_003826"
     CDS             complement(4424..5350)
                     /gene="tauA"
                     /locus_tag="OO566_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6606916.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDDKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(5617..6132)
                     /locus_tag="OO566_003827"
     CDS             complement(5617..6132)
                     /locus_tag="OO566_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606917.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            6325..>6491
                     /locus_tag="OO566_003828"
     CDS             6325..>6491
                     /locus_tag="OO566_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6606918.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERV"
BASE COUNT         1601 a         1720 c         1572 g         1598 t
ORIGIN      
        1 tcagttgtca tgcactcgga ataaatcata ccgtgaatag tgctgttttt gattatcaga
       61 ctattggcgt tggtattggt agaaataccg tcgtccagat agtaggtaga aatcacgccg
      121 ttaacggtag cgttattaat gaccgggtag atatcgccgt tgtaatagct atcagcggca
      181 tagttgttcc agcctacata accatcatag taaacgtcat cagcataggt ggcgtcgttg
      241 tagtgatgga aagtattgta agtttgacca gaaatatcgt tggtcgctgc attagcctga
      301 gaggtgatag ccagagtgca agccaatgct aattgtgata ctacaagttt ctttttccag
      361 gagtgcattt gtcatccccc tcagggacgt aataagttga tccatcaatt atcaatgcat
      421 agaaaacata ttatgtttcg cgctaacgat tatgctgcgt tacagacgaa tggttcaatt
      481 attctttccc ctaagtccga atgtggacat atataattta tggagaaaaa cacgcatcaa
      541 attgataaaa ggacgatatt tcagcatgtt gcagataatg ttatttttga tgcatacgcg
      601 ttataaatat caaaaacaca aaccagataa atatcttatc aatatacaac aaagtttgaa
      661 ttattcaatt tatagcagca caaccaaacg ccaaaaaaat aataaccaag agctataaat
      721 aaagattaaa aatattttca gtgaatatga aattattgct atttctatta caaagatgtc
      781 ctggtctctc tttcattctc ttcttgtcaa catcgcgaca actttcgtaa aacactcctg
      841 ccccgcttca ggtatactgt gcaccttaat tccacaaaca tcaggcagac catgacagac
      901 ttaatccaac gccctcgtcg cctgcgcaaa tctcctgcgc tgcgcgctat gtttgaagag
      961 acaacactta gccttaacga cctggtgttg ccgatctttg ttgaagaaga aattgacgac
     1021 tacaaagccg ttgaagccat gccaggcgtg atgcgcattc cagagaaaca tctggcacgc
     1081 gaaattgaac gcatcgccaa cgccggtatc cgttccgtaa tgacttttgg catctctcac
     1141 cataccgatg aaaccggcag cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg
     1201 cgcatctgca agcagactgt gccagaaatg atcgtcatgt cagacacttg tttctgtgaa
     1261 tacacttctc acggtcactg cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact
     1321 ctggaaaatt taggcaagca agccgtggtt gcagctgctg caggcgcaga cttcatcgcc
     1381 ccttccgccg cgatggacgg ccaggtacag gcgattcgtc aggcgctgga cgctgcaggc
     1441 tttaaagata cggcgattat gtcgtattcg accaagttcg cctcttcctt ttatggcccg
     1501 ttccgtgaag ctgccggaag cgcattaaaa ggcgaccgca aaagctatca gatgaaccca
     1561 atgaaccgtc gcgaggcgat tcgtgagtca ctgctggatg aagcccaggg cgcagactgc
     1621 ctgatggtta aacctgctgg agcgtacctc gacatcgtgc gtgagctgcg tgaacgtact
     1681 gaattgccga ttggcgcgta tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg
     1741 ctggcgggtg ctatagatga agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt
     1801 gcgggtgcgg atctgatttt cagctacttt gcgctggatt tggctgagaa gaagattctg
     1861 cgttaatttt tttcctctca gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc
     1921 cctcacccta accctctccc caagggggcg agggaaccgt ccacactcgt atcaccccgc
     1981 ccgataaaac ggtttatccc caaggatcgt cgcccgatgc ataatccgcc gctgtggcag
     2041 gtaatcggca ttggcatagt gctgagtcac gcggttatcc caaatcgcaa tatcattcgg
     2101 ttgccagcgc caacgcacct gaaactccgg tttagtgata tgggcgaata aaaagcctaa
     2161 caaggcttcg ctctctttct cgctgacatc gacaattcgc gtagtaaagc cttcattcac
     2221 aaacagcgcc tgtttaccgc tcaccggatg cgttcgcacc accggatgta gcaacggagg
     2281 atttttcgcg accgcctcgc accagcgttg atgttcctcc tcggttttgc ggtatttgta
     2341 ttccgggaat gatttacgga aatcatgctc cgcacgcagc cagctcagca gctggcggaa
     2401 gggaacagag agcgcctcat aggccgcaat accgctggtt cagagcatat caccgccggt
     2461 cgaaggtaac tctttagctg ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac
     2521 atcggtatgc cagttgtcgt tatccggcgg attatcgtta tgggtatcca gcacgatgat
     2581 ctcgtcaacc ccttcggcat gagggtaaac agggtgaata tgcagttcgc caaaacgctg
     2641 ggccagcgcg cgttgctgct gcggcgtaat agcttgatcg cgcagaaata ccacctgatg
     2701 gcgcagcacc gcatggtaaa gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc
     2761 ggcacccgaa atttgtgcgc cgatatgcgg ccccagcggg gtaatgctca gacgttcacc
     2821 cattgtactt ctccatgcca gggcgtcaga cggcgctgta acgcgcgcag acccagttct
     2881 aaaagaaagg cgataatcgc aatcaccgcg atccccgcca gtaccacgtc agtcgcgaga
     2941 aattcacccg ctgactgaac cataaatcct aaaccgcgtg tcacggcaat cagctccgcc
     3001 gctaccagcg tagaccagcc cacacccaga ccaatgcgta atccggtgag gatttccggc
     3061 agcgcgccgg gcaaaatgac aaaccacagc acctgcgcac ggctggcacc cagcgactgg
     3121 gcggcacgaa tgcgaacctg ctgcgcgctt ttcacccctg ccagcgccga catcgccacc
     3181 ggcgcaaaaa tcgctaaata gatcagtaag atcttcgagg tttcaccaat accaaaccag
     3241 atcaccatca gcggcaaata agccagcggt ggcaccggac gataaagttc gattaccgga
     3301 tccagaatgc cgcgcaccgt cgggcttagc cccatggcga tcccgaccgg aataccgatc
     3361 accaccgccg cgagtagcgc ctgcacaatg cgcgtcaggc tggcagccag atgctgccac
     3421 agcgtggcgt ccataaatcc ctgcggtccg gcaatggtga gcagttttgc cagtacttgt
     3481 tgcggcggcg gcaaaaacag cgggctaatc agttgcagcg ccgccaccgt ccaccataca
     3541 gtgagtaaaa ccgctaacgt gccaatgctt aaggtcacct gacgcgagag cggccagcgc
     3601 catttcagcc gccgcgaatg cagtttttca ttaatgagca cactcatgag aacgcctccc
     3661 gttgctcaaa tacgcggctt aaaacatatt cgcgcatggc gatgaattgt ggatcggact
     3721 tgatgctgcg gctcgactct cccgcaacaa agcggcgagc aaagttgagc ggcagccgct
     3781 ccagcacacg gccagggccg gatgaaagca gaaccagttc agtcgccata aacaccgctt
     3841 cttctatatc gtgggtaatc aacagcacct gcttgcccgt ctcctgccag agtttcagca
     3901 gcagagtttg catctggtcg cgggtgaagg cgtccagcgc accaaacggt tcatcgagta
     3961 ataacagctg gggattcgcc gccagcgcgc gggcaatccc cacccgctga cgttgaccat
     4021 cggaaagctg ccagatatag cgtttttctg cgccttccag ccccactttt ttcagcatct
     4081 ggtgcgcgat ttccagtcgc tgcattttct ctatacctgc caattgcagg ccgaacgcca
     4141 cgttgtcctg tacattgcgc cacggtagca gcccttcatt ctgaaaaact acgccacgct
     4201 ctgctcccgg tccctcaata cgcttacccg ccagttgaat gctgccatgc tgataaggca
     4261 caaaaccggc aatcagattc agcagggtgg ttttaccgca gccggacggc cccagcacca
     4321 ccagtagctc gccgctttcc agcgtcaggt tgatatcttc cagtgccggt ttgccgccat
     4381 aatcggcata gagatgagag atttgcagca tctgcgcctc cttttattgc acgaagcgcg
     4441 aggtaacgta ctggctgtaa tcattcgcta cagccgggac cttgccctgc tctttcaaaa
     4501 actgcgcggt gtcgatgatt gctttgttca ccggtccggt cagttctgcc gtttgttgct
     4561 gcggcgtcag ataggtattt cccttcacca gccccggaac gtcaccttca ggcacgccgc
     4621 ttaaacgcgc cagtttgctg atgttttccg gctgtttcag ccacgcgtca gggttagcaa
     4681 tgtacggttg ctgtgcatcg atggcgcttt tggcgaacgc tttcacgacc tcaggatgtt
     4741 tctcggcaaa atctttgcgc accacccaga cgtccagcgt tggcgcaccc cactgcccga
     4801 cctgttcaga atcggtcagc accttgtcgt ctttttccag ggcgttaacc gccggtgccc
     4861 agacataagc accatcaata tcaccccgct gccatgcggc gataatcgcg ggcggctgca
     4921 ggttcacaat ctccactagc cctggtttaa tcccccagtg tttcagcgcc gccagcaggc
     4981 tgtagtgggt ggtggagata aacggtacgg caatgcgttt accaatcaaa tcttcgggtt
     5041 tgctgatagt tttctttacc accagcgctt cggagttacc cagttttgac gccagcaaga
     5101 agacttcaat cggcacctgt tggctggctg caaccgctaa tgggctggat ccgaggttgc
     5161 cgatttgcac gtcgccagaa gccagcgccc gcacgatgct ggctccgctg tcaaacttac
     5221 gccagtccac gctcgccccg ctctctttag caaaggtgtt gtcggcctga gccactttcg
     5281 ccggttcggc tgaggtttga tacgccacgg tgacgttcac cgcctgtgcc tgaaaagcga
     5341 tgaatgccag tgcggcaaga agtgtgttat atgactaggt aatttattca agataaccgg
     5401 gaatgggtaa aaaaagacgg gatttgatgg gattcaataa gttatgcgcc gattgcattc
     5461 aagataaaac gagttaaaaa tgctcaatgc ttcgatatcg ataaaacatt caagataggc
     5521 gcgccgtgta ttttaattct tttaagactc ttcaaaaatg tcatcgtagc ctttaaaaac
     5581 gaattttaaa ggcaccctaa aggcaggtca atgaagttaa cccgccttcg cgtttacact
     5641 ctctgagaga atactagctt caatctcttc caaaatctct ctgacttgct cgtcgggcat
     5701 atcaacgagc atcaacgctg ccaggcgttt acggccctct agttgcagta gctcttgagt
     5761 ttccttatct aacaagcgta gcacagtctc aacacctttc cttgcgagca tgtttgtaag
     5821 ggcttctcgt tcctcactgg taagaaacga caacatttct gttagctgat ccccttggct
     5881 taaatcactt tgtgtttttt gtgtttttgg tggattcccg ttaccggttt ttagccattc
     5941 aagactaaca ccttcacttt ctgcaatgcg taccaatatg ctttctcgag gttctggtgt
     6001 atttcccttt ttaaaatagc tttgtaacgt attgatattt atgccacagg ctctggcggc
     6061 agcacttctt gatggatacc gcctaataag ataagccatg cgctccacga taggttcttt
     6121 tgcctgctcc ataaaagtac ctctaggtat ttttctgtat aaaggttctt ttccatctat
     6181 ctaattgaat ggaaaggtaa ttttaaaaat ccaaataaaa tacaaaaaaa taccttgtat
     6241 tggtgctttt tgtgatctgc gattttattt actcggatat ccacgcgaat atccgcgcgg
     6301 gtaattttgt agggtaaagg aaagatgaga caaagagaag atagtggccg cgattggcct
     6361 tcgctgatga ttcagcaagc actcaaagat aatgggtggt cttcctttcg ggagctttca
     6421 ctccatgccg gttatgctca ggattcactg aaagcggtag ttcgtacgcc aaacaaaaaa
     6481 tacgaacgtg t
//
LOCUS       DAJTXY010000174         6172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTXY010000174 DAJTXY010000000
VERSION     DAJTXY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.176"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            172..819
                     /gene="fucA"
                     /locus_tag="OO566_003829"
     CDS             172..819
                     /gene="fucA"
                     /locus_tag="OO566_003829"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6606919.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            847..1995
                     /gene="fucO"
                     /locus_tag="OO566_003830"
     CDS             847..1995
                     /gene="fucO"
                     /locus_tag="OO566_003830"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6606920.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2048..2803)
                     /gene="xni"
                     /locus_tag="OO566_003831"
     CDS             complement(2048..2803)
                     /gene="xni"
                     /locus_tag="OO566_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6606921.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTLAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2914..4281)
                     /gene="sdaB"
                     /locus_tag="OO566_003832"
     CDS             complement(2914..4281)
                     /gene="sdaB"
                     /locus_tag="OO566_003832"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6606922.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4339..5628)
                     /gene="sdaC"
                     /locus_tag="OO566_003833"
     CDS             complement(4339..5628)
                     /gene="sdaC"
                     /locus_tag="OO566_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6606923.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1571 a         1507 c         1644 g         1450 t
ORIGIN      
        1 aagagaactt gcagttattt actgtgatta gttgagctag gtcacaaaat ataagcttgt
       61 gaatttcttt cttgttaatc aggaagaaga tggtgggtaa ttaaacggct aattcaatag
      121 tgtgaaagga acaacattat tgccctgttt tgaatcagag agaggtagct aatggaacga
      181 aataaacttg ctcgtcagat tattgacact tgcctggaaa tgacccgcct gggactgaac
      241 caggggacag cggggaacgt cagtgtacgt tatcaggatg ggatgctgat tacgcctaca
      301 ggcattccat atgaaaaact gacggagtcg catattgtct ttattgatgg caacggtaaa
      361 catgaggaag gaaagctccc ctcaagcgaa tggcgtttcc atatggcagc ctatcaaagc
      421 agaccggatg ccaacgcggt tgttcacaat catgccgttc attgcacggc agtttccatt
      481 cttaaccgat cgatccccgc tattcactac atgattgcgg cggctggcgg taattctatt
      541 ccttgcgcgc cttatgcgac ctttggaaca cgcgaacttt ctgaacatgt tgcgctggct
      601 ctcaaaaatc gtaaggcaac tttgttacaa catcatgggc ttatcgcttg tgaggtgaat
      661 ctggaaaaag cgttatggct ggcgcatgaa gttgaagtgc tggcgcaact ttacctgacg
      721 accctggcga ttacggaccc ggtgccagtg ctgagcgatg aagagattgc cgtagtgctg
      781 gagaaattca aaacctatgg gttacggatt gaagagtaat ttcgtaaagc aacaaggaga
      841 aggatgatgg ctaacagaat gattctgaac gaaacggcat ggtttggtcg gggtgctgtt
      901 ggggctttaa ccgatgaggt gaaacgccgt ggttatcaga aggcgctgat cgtcaccgat
      961 aaaacgctgg tgcaatgcgg cgtggtggcg aaagtgaccg ataagatgga tgctgcaggg
     1021 ctggcatggg cgatttacga cggcgtagtg cccaacccaa caattactgt cgtcaaagaa
     1081 gggctcggtg tattccagaa tagcggcgcg gattacctga tcgctattgg tggtggttct
     1141 ccacaggata cttgtaaagc aattggcatt atcagcaaca acccggagtt tgccgatgtg
     1201 cgtagcctgg aagggctttc cccgaccaat aaacccagtg taccgattct ggcaatcccc
     1261 accacagcag gtactgcggc agaagtgacc attaactacg tgatcactga cgaagaaaaa
     1321 cggcgcaagt ttgtttgcgt tgatccgcat gatatcccgc aggtggcgtt tattgatgct
     1381 gacatgatgg atggtatgcc tccggcgctg aaagccgcga cgggtgtcga tgcgctcact
     1441 catgctattg aggggtatat tacccgtggc gcgtgggcac taaccgatgc actgcacatt
     1501 aaagcgattg aaatcattgc tggggcgctg cgaggatcgg ttgctggtga taaggatgcc
     1561 ggagaagaaa tggcgctcgg gcagtatgtt gcgggtatgg gcttctcgaa tgttgggtta
     1621 gggttggtgc atggtatggc gcatccactg ggcgcgtttt ataacactcc acacggtgtt
     1681 gcgaacgcca tcctgttacc gcatgtcatg cgctataacg ctgactttac cggtgagaag
     1741 taccgcgata tcgcgcgcgt tatgggcgtg aaagtggaag gtatgagcct ggaaggggcg
     1801 cgtaatgccg ctgttgaagc ggtgtttgcc ctcaaccgtg atgtcggtat tccgccacat
     1861 ttgcgtgatg ttggtgtacg caaggaagac atttcggcac tggctcaggc ggcactggat
     1921 gatgtttgta ccggtggcaa cccgcgtgaa gcaacgcttg aggatattgt agagctttac
     1981 cataccgcct ggtaaatgcg ctgatgtgat aatgccggat acgatttgcg gcgtatccgg
     2041 ctcgccgtta ccgtaccaac cgcaattgct gtaaattgcc gtcgatatgc aaatcggttt
     2101 gtaagcgggc aatatcgcgg cacagaaacg ccatctcttt atgggtttct aattttttgc
     2161 gccacttttc ggcaaccgca tccagattct catatatccc ttccagactc tgaaactcga
     2221 ccagcagctg cgtggcgctt tttggtccga ttcccgcaac acccggtacc tttgaactgc
     2281 tgatccccgc aagtccccag taatcgggca actgctgcgg ttgaacgcca aattctttat
     2341 cgataaatgg cgcatccagc caacgtttct ggaagtaatc acgaatacgt aatgtcggtg
     2401 aaagtaactg acagtagcct ttatctgtcg aaacaatcgt tgcctgatgc ccggccagtg
     2461 tcactttgac cgccagtgtg gcggctaagt catcggcttc gttgccgctg gctgaccagc
     2521 acgggacgcc gcgttgctca aaggcggcgc gtaatgcagg catctcgtcg tgcaactctt
     2581 ccggcattgg cggtcgatcc gctttgtaat ctggtaaacg ctgatgacgc cagccgctac
     2641 tgcggttttc atcatcaaaa acggcgaccg cgtgggttgg ctggctgtgc ataatgagct
     2701 gatcgagcgc atgctggcag gtttcgacac agggcgaacc ctgaacggca tgaatgcgac
     2761 ggataagatt cagtgcatcg acaataagca aatgaacagc cacgataacc cccttaaaga
     2821 tcaggcgata agggtaacat tcgatgccgg aacaggctat ggctggttga gaaaacagga
     2881 agaggcttcg caaaacgagg cctctgggag agattaatcg caggcaacga tcttcatcgc
     2941 caggccgccg cgagaggttt cgcggtactt ggcgttcatg tctttacctg tttcgtacat
     3001 ggtttcgata actttatcga ggcagacgcg cggttcgctg gtacggcgca gtgccatacg
     3061 cgcggcgttc accgctttca ctgctgcaat ggcgttacgc tcaatgcacg gtacctgaac
     3121 ctgccccgcg accgggtcac aagtcagacc gaggttgtgc tccatcgcga tttccgctgc
     3181 gatgcaaacc tgcgccgggc ttgcgcctaa cagttctgcc agaccagctg ccgccataga
     3241 gcacgccacg ccaacttcac cctggcaacc cacttcggca ccagaaatcg acgcgttcat
     3301 cttataaaga gaaccaatgg cgctggctac cagcaggtaa cgagccagtg agttagcgtt
     3361 cacttcgcgg ataaacttat cgtagtacgc cagcacagcc gggataatcc cgcacgcacc
     3421 gttagtcggc gcagtcacca cgcgaccgcc agcagcgttc tcttcgttca ctgccagtgc
     3481 aaacatgttg atccagtcaa caaccgccat cgggtcagtg gtggttttat cctggctgac
     3541 cagcatccgg cgtagtgccg cagcacggcg tggaacgcgc agtttgccag gcaacacgcc
     3601 ttcggtggaa ataccgcgct caataccgcc acgcatgact tcccagacgt tcgccaggtg
     3661 ctgttccagc tcttctttac tgtgcagcgc cagctcgttt ttcatcatca ggccagagag
     3721 tgacagcccg gtttcctgac aatgtttttg cagatcggct gctgaactgt acggataagg
     3781 aacttcaacc ggtgcgctat cctgctggcc aaaatgctct tcatcaacga taaagccacc
     3841 gccaatagag tagtaagtct ggctgtaaac gactttatcg cccgccagcg cggtaatgcg
     3901 cataccgttt tcatgcagag aaaggttgtc tgcatggaag ttcatgcact gatcaaccgg
     3961 gaactccact tcatgctgac cgtttgccag catcaggcga ccatgagtat tcacatcctg
     4021 aataaaaccg gggatggaat cgatatccac ggtatccggc aggttacccg ccaggcccat
     4081 aataatggag atatctgtgt ggtggccttt accagtcaga gagagcgagc cgtacacgtc
     4141 aaccaccacg cgggtcacgt ctttaagcag gttacgggca atcagatcgt cggtaaattg
     4201 tttacccgct ttcattggtc caacggtatg agaactggaa gggccaatgc cgattttgaa
     4261 aatatcgaat acgctaatca taggaaatac atcgcgttaa aacggaggaa gcgccgcccg
     4321 aaagcggcgc gaaaggactt agctgaacag agagtagaag attgcggaga ttgcaatcag
     4381 ccccatcacg acaacgaata cgttgctgat gtgaccgctg tacttacgca ttgccggtac
     4441 tttctgaatt gcgtacatcg gcatcaggaa caggatcatc gcgatgattg ggccgcccag
     4501 ggtttcaatc atacccagga tgctcgggtt cagggtggca acaatccagg tcgttaccag
     4561 catgaacagc gcagtgatac ggttcagctt gttgatttcg atagacttac ctttaccacg
     4621 cagagattta atcaccatac cgttgaagcc ttcacgtgcg cccaggtagt ggccgaggaa
     4681 ggatttggtg atagcgataa tcgcgataat cggagccatc cacgcgataa ccggtgcgtt
     4741 aaagtggtta gccaggtaag acagaatcga gatgttctgc tctttagccg cagccaggtc
     4801 tgccggagtc aggctcagta cgcagctgaa gacgaagaac attacggtca gcaccatcat
     4861 gatgtgtgcg aatgccagga tcttcgagca tttctgttct gccatatcgc cgtactcttc
     4921 acgcttcgca acggcgaaag aagagatgat cggagagtgg ttgaacgaga acaccattac
     4981 cggaattgcc agccacaggg tcatccacag accgtttccg gttgcagatg cagtgtccag
     5041 agacagcgtt tccagtgctg cgccgttcca ctgcgggatc aggtacagag ccagcagcat
     5101 cagtacgcca acaaacggga ataccagaat actcatcgct ttaacgatca tctgctcacc
     5161 aaagcgaacg atggtcatca tacccacgat caggatcagc gacagaatcg cacgcggcgg
     5221 tggcgtcata cccagctggt gagacatgaa gctttcaacg gtattggtga ttgccacgct
     5281 ataaaccagc aggatcgggt agatagcgaa gaagtagagc agggtaatca gtttacctgc
     5341 gccaatacca aagtgttctt ctacaacctc ggtgatgtct tcgcccgggt ttttaccaga
     5401 cagtacgaag cgagtcaggc cgcggtgagc aaaaaacgtc atcgggaacg caaggatagc
     5461 catgatgatc agcgggatca taccgccaac accggcgttg attggcagga acagcacgcc
     5521 cgcgccgatt gccgtgccgt aaaggcccag catccacatg gtgtctgtct tgcgccaggc
     5581 actacgagag tctttcgacg caatcgtgct ggtttgagtc gtttccatct atttctcctg
     5641 gaggaatgca aataattcag cttttagcca gttcagcccg ggaaggtgcc tgaccgtaaa
     5701 aatgaaatct gtctatcggc gatggcttaa tcatttaccg ctgtaataat ttgagggcgg
     5761 aaagatacac acaagttatg tatctatcag tgatcttgat cccaaataca ataatctgct
     5821 tggaagatag attatcccaa cgctttactc tgtaaatcaa aagtaaaaga gaatttaagg
     5881 gcaatatttg cccattaaac gacatctgac ttcttccggg gcgcgcaata tatgtaggtt
     5941 gaactgaaaa aatcaatact tttagttaat catggcgata atttcttttt catagcgata
     6001 aaatacggtt tatgaaaata gggaaacgtt tgcgttgcaa cataaattgt atggcatatt
     6061 aactgtgatg atatttgtcg cgtgagggtg gtaagaaaca ttcatataac aaatgaaatg
     6121 agttgatgag taagtgacca aaaacgagaa aacccggcaa caaacgtcac cg
//
LOCUS       DAJTXY010000175         6157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTXY010000175 DAJTXY010000000
VERSION     DAJTXY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.177"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..385
                     /locus_tag="OO566_003834"
     CDS             <1..385
                     /locus_tag="OO566_003834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606924.1"
                     /translation="ENIIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLY
                     PVKITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITN
                     KKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(461..925)
                     /locus_tag="OO566_003835"
     CDS             complement(461..925)
                     /locus_tag="OO566_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6606925.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(922..1797)
                     /gene="nanK"
                     /locus_tag="OO566_003836"
     CDS             complement(922..1797)
                     /gene="nanK"
                     /locus_tag="OO566_003836"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6606926.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1794..2483)
                     /locus_tag="OO566_003837"
     CDS             complement(1794..2483)
                     /locus_tag="OO566_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6606927.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2531..4021)
                     /gene="nanT"
                     /locus_tag="OO566_003838"
     CDS             complement(2531..4021)
                     /gene="nanT"
                     /locus_tag="OO566_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6606928.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4131..5024)
                     /gene="nanA"
                     /locus_tag="OO566_003839"
     CDS             complement(4131..5024)
                     /gene="nanA"
                     /locus_tag="OO566_003839"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6606929.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5146..5937)
                     /gene="nanR"
                     /locus_tag="OO566_003840"
     CDS             complement(5146..5937)
                     /gene="nanR"
                     /locus_tag="OO566_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6606930.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1551 a         1674 c         1545 g         1387 t
ORIGIN      
        1 ggaaaatatc atcagatggc tgcttaaaaa aagaacaaat ggtagtgtcc gctattgcca
       61 gtacacctca aaagttgcca tgatttacat tgaacagctt gaattaatcc ataaaagtgg
      121 tgatgtgttg tatccagtaa aaataaccag aaaatcatca ggaaaaactg ccttccacct
      181 tgttccattc ggcttaaata agactcacga ccttctagag gtggaagatg cttcagaagc
      241 aattcggctg gtgattgatg aacgtcattc cattcgctgc agtacactta cagcaactat
      301 tacgaataaa aagggcaagc gaattaagcg gacaggtata tacagtatca agggtgttaa
      361 cataaaagaa tacaacgtca ggtaatggca cagagcggtc cgtcagttca ggtttggctc
      421 tgtgccaaag atgggtcagg tcacacctga tctaataaat ttaagccatt aaatcagcct
      481 taaccttcac caccaccttt ttaatctcac ccggctcgcc aacaacgcat cccggcttat
      541 gcggctctcc tggcataaac acggcgaaca ttcccggttt caggatgatg gtttgctcgt
      601 tttcaatggc gctacaaagc tggtaatcat cttcatggtg gaactcttca cattggcgcg
      661 cagtgcctgc catgccaaac agaatccgtt cctcaccgtt taataacagc tggatatcaa
      721 tgtattgctc gtgcaattcc gcttttttct cgacgggcga ttgagtgtta aacgtcatga
      781 cattcataaa aatattgtcg ccctgtaatt cgtaacgacc cggcgctttt tcttgcggtc
      841 tggcagctaa ggccagcgtt aacgcgtcct gtaacgcagg atgtaaccca gcagacggta
      901 atgactgtac ttcacccatc atcataattt ttctccctgg gccaacagcg cagccccaag
      961 taaacctgca tcatggcggt aatgcgccgc cagtaaatca acatgaaatg ccgctggctc
     1021 ctgcgccaga tacatttcca ccaacgccag atacccttct gccagaccaa cgctgccacc
     1081 aaccaccacg cactggcaat cagttgtggc tttaatatca gcgatcagcc ttgcaagcgt
     1141 acgtgcggag cgatgaatca gttgctgcgc ctgctcatcg ccctgcccgg cgcgtgtgaa
     1201 aatagttctc gcatccgcgc cagccagctc gccctgcgct gccgccgcaa tgccgcgacc
     1261 agaagcaatt gcttccacgc aacctgtgcg tccacagccg cagactgggc cgtgtggatc
     1321 ggcaagcgta tgcccgatgt gccccgccag accgccaggg ccggtaagca gtttgccgcc
     1381 gctcactaca ccgccgccga cgccagtgga aacggtgata aagaccatat cggttatatc
     1441 gccttccagc gcctgatact ctgcccatgc tgcggcctgc gcgtcgttaa tggcaatggt
     1501 cggcaaattg gtaagttgtt ccagcgtttt gactaacgga aagtgtagca atccaccgag
     1561 attatgcgga ttcagcgcca gcaaactgcc gtcgcgaata attccggttg aagcgatggc
     1621 aacccgctgc gcatgagctt gcaacggaga gactaatgcg gataaggcat cacgcaaggc
     1681 ttctggtgtc tggctggctg gcgtaggaag ttcacgacga tcgcggatct gcccgtcagc
     1741 gccaatcagc gcggcggcaa gtttagtacc gccgatatca atcgccagta tggtcatagc
     1801 accgcctttt tcatcgctgt gttgtaccac tgacaaatgt gttcaagacg cgtaatggca
     1861 gaaccgaccg tcaccgccca cgcgccgtgg cgcatcgcat ctgccgcctg agcaggcgtg
     1921 ttgtaacgcc cttcggcaat cacccgacat ccggcttcgc tcaacgtttt caccagcgcc
     1981 agatccggct cttctggcgt ttcaggcgtg gtatagccag aaagcgtagt gccaataatt
     2041 tcggctccca gcttttggca tgccaggccg tcttccggcg ttgagcagtc ggtcatcgcc
     2101 agtaaaccgt gatgatggat acgtgccagc agcgtttcaa caggcaccgg acgcgggcgg
     2161 tcggtgccgt caatggcgat aatgtccgcg cccgcctgcg ccagcgcatc gacatcttca
     2221 atataggccg tgatgcgtac cggagaatcc tccagatcgc gtttcacaat tccaataatc
     2281 ggcacgctca ccaccgcacg cgtggcttgc agatttgcca caccttcgat gcgaatggca
     2341 accgcgcccg cctgctctgc cgctaatgcc atggctgcga cgatttcggg tttatcgagc
     2401 gggctgtcag gaaccggctg gcaggagaca atcaggccac cgttagcagc gattttttga
     2461 tccagttgtg caagtaacga catacatctt cccttagcga aaggcccggt acatagaccg
     2521 ggcaacagga ttaacttttg gttttgacta aatcgttttt ggcgctgcca aatggcacgg
     2581 caccgctaaa tggtttgccg tcgatagcgt catgagtacg caatgcttcc gggcgcagcc
     2641 aacgctgaac gcgggaaggc atatccagac caatcagcag gatcaccaca aatgtcaggc
     2701 tgaacgagag cgatgccagc gcagtaccca gatccagacg ttgagcgatc aacgcgccga
     2761 tgattggggc cagtgcgcca cccaatgcgc caacgttgta ggtaaagccc aggcccgctg
     2821 cacgctggtc ggtatcgaaa taaccgccaa tcagtttcgg taagatcccg gcgatccctt
     2881 gtccaagcat ttgctggaag aacagtaaca gaccgagcac ccagacgttt gcgccgccaa
     2941 tcgcaaatac cggaataatc agcagctgcg aggccagcag gctacaaacg tacgctttgt
     3001 gggttcccag ccagtcaccg aggaagccac ctacgcagca tcccacggcc gcgccaaagc
     3061 cactaaagaa cagcacattg gcgacagtat gcgggttata agccagatcg gttttcagat
     3121 acgttggcag cagcgcctga atcggccatg agtagaggaa agcaaacaac acgaccacca
     3181 tcagcattac gcccgttggc cagcgtttac ccgtactttg caccataaag ctgataaaga
     3241 ttgcggcgca taacagcccg agaacagcga cgattgcagc attttgcagg ttaccggcga
     3301 agcagaacca cagcgcagta gccgccgcca gtgtcattac gatattggca atgcgatgtt
     3361 caccacggta gagaatatcc accattgtgc gtactggtgc tttacctgcg tgtttctctt
     3421 tccagtcttc cgcttccggg atgtttttac gcagccagag agcaaagatg attggcaaaa
     3481 tgccgataaa gaacagcgca cgccaacccc agaccggaac caccaggcta tagacctgag
     3541 cggcaacgac ggcccccaca gagaagcctg aaatcaaaaa accactggct ttgttacgca
     3601 gatgttttgg ccagctttca atgacatagg tggcgctgga accgtattca cccgccatcc
     3661 ccatgccgat gaccagacga gcgataaaca tggtgatgta gcctggcgca aagccgcagg
     3721 ccagcgtccc ggccgagaag agaacgatgc tggtgaccat tgccagacga cgcccgtagc
     3781 ggtcacccat agcgccgagc atcaggccgc cgaaccagcg agagataaag gctgcggaga
     3841 tcagacttgc cgcctgcacc gtcgtcagcc cgaattcacc ctgtacttcg gtgagtacca
     3901 gggcgattaa aacgaaatca aaaccgtcaa gcagatatcc caaccaggca gcggaaaatg
     3961 cgcgccattg tgcacggttg agatggcgat accacgggat attctgggtt gtagtactca
     4021 ttgtgagtct cccgcggtgg gcgatgccca cacgctttgg tatgaaaatt gtagggtaca
     4081 gatgcgttta tttcccctca cccggtaggg gcgagcgagg ggaaaacaac tcacccgcgc
     4141 tcttgcatca actgctgggc cagcgccttc agttctggca gatatttttc atctaccggt
     4201 ccaaacggtt tgcggcacag cggcacagaa acgacatcca tataatgcag gacagttttc
     4261 aggccgcgga atacgcccgt tttgatcagt aaatcaatga ctttgttgca ctcagtttgc
     4321 agtttctgcg cggtctggat atcgccttct ttcagcgcct tcacgatccc ctgatatcgc
     4381 cagcccatga tgttgtaagt actgccgata ccaccatcag cgcctgccag caagccggaa
     4441 gcgaagattt cgtcgtaacc gttatagagc accagatcag gatgttcacg acggatctgt
     4501 tccatctgat agagatcgcc agaagtctgt ttcagcgcac cgacgccagg caacgtaacc
     4561 agggtgttga tctgatccag ggtcagtttt accccactca gggctggaat gttgtacacc
     4621 accatcggca aaccatccgc tgaatcaata attgcccgat agtgatcgca gtgttcttca
     4681 aagctgaaag gatagtagaa cggcgtgacg gcggagacgg catcgaagcc ataacgttta
     4741 gccgatgccg caagttgttg gctttcagcg gtgctgacgc aaccgacgtg ggcgatgagt
     4801 ttaatcttac ctttcgcctc ttcggcgacg atttccagta cctgttcacg ctcggaaagg
     4861 ctttgtacaa aggcttcgcc ggtcgaacca cccacgtata aaccgtcgat gccctgctga
     4921 atattgaact gaaccaggcg gcgcagactc gctttatcca gtgcttgttg ttgatcaaaa
     4981 ggagtcagga gtgcagccat tacgccacgt aaattcgttg ccataaatac ctctgaagtg
     5041 atgcttgtct gataaacgat atacctttat acctgttata ccagatcaat taagcaacac
     5101 cccatacaga aagcttataa tgcgatctgc ttcactaaag tggcattatt tctttttgtt
     5161 ggtggtctga ccgaaagcgt gccaggtagc agagacgctg ttgagatgcg attgcaacgc
     5221 acgatcggct tcgtcaggat catgacggcg gatcgcatca acgatcgcaa tatgctgttg
     5281 ataactaacg ttgttatgtt cgtgcagtgc ctgatcggta accgttgggc gtgcggcaat
     5341 aagccagtcg agcagggcaa cgtggatcgc catgaagatt gggttaccgg ggatctccgc
     5401 cagcacgcgg tggaaatcaa cgtctgaacg aatgaatgcc gcgttgttat ccagcgactg
     5461 actgttgatt tccagtgctt ttgccagcaa atcgatttgc tcatcggtgg cgtgttcagc
     5521 cgcatagcgc accaggctgg attcaaagaa cagacgtaat tgttcgaaat gggcaatccc
     5581 accaggatga gaaaggaaat ctttcgccat gccggaaagc tcgccgatga tggtgtccgc
     5641 agaaggtcga gagacacgag cgcgttcgcc gttgtttatt tgcaccagac ctttgcgttt
     5701 taacgctgcc agcgcttcac gcacagaagg acgcccgacg ttaaagaacg ccatcagttc
     5761 gcgttcagac ggtaattgtt caccttcgcc aaattcacga cggcggatca tctgttccag
     5821 ctcttcttcc accatttcgg agagtttttt acgcgccagc gggcggctac gcaagttgcg
     5881 accaattgca ggtgaagaat cttcggtttg cgaatcaaat gcgttcataa ggcccattct
     5941 gtaaggtcag tgtgattaac atcatcagtg acatcctatc acaggattga aagtagggga
     6001 aaatggcagg tttttctctt tgtacctcat cattaccata attaacggaa taattaacta
     6061 ttgcgaaaaa ttaatgtaac gcagataaac acatcccgat tgaattattc ataagactat
     6121 tcacaagcat tatgaatatt atgaatgtgt tcttaca
//
LOCUS       DAJTXY010000176         6136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTXY010000176 DAJTXY010000000
VERSION     DAJTXY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.178"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            602..1780
                     /gene="ltrA"
                     /locus_tag="OO566_003841"
     CDS             602..1780
                     /gene="ltrA"
                     /locus_tag="OO566_003841"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6606931.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDVTRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1863..2042
                     /locus_tag="OO566_003842"
                     /pseudo
     CDS             1863..2042
                     /locus_tag="OO566_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2072..2554)
                     /locus_tag="OO566_003843"
                     /pseudo
     CDS             complement(2072..2554)
                     /locus_tag="OO566_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2810..3790)
                     /locus_tag="OO566_003844"
     CDS             complement(2810..3790)
                     /locus_tag="OO566_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6606932.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3790..4989)
                     /locus_tag="OO566_003845"
     CDS             complement(3790..4989)
                     /locus_tag="OO566_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6606933.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5311..5649)
                     /locus_tag="OO566_003846"
                     /pseudo
     CDS             complement(5311..5649)
                     /locus_tag="OO566_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5716..6136)
                     /gene="istB"
                     /locus_tag="OO566_003847"
                     /pseudo
     CDS             complement(5716..6136)
                     /gene="istB"
                     /locus_tag="OO566_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT         1664 a         1409 c         1461 g         1602 t
ORIGIN      
        1 gccgggcagc ggtaaaacaa ggactgcccg gctgtgggtc tacgttcgtg atgaccgtaa
       61 cgccggttcg gaaattgcag cataagttat ccatgctctg aggtgggtgg aggtgggccc
      121 tccggtcagg gtttacagga gcactgacca aacgacccgg tgctgcttca ttctaaggaa
      181 tgaggtggtg agcgactcgg gaaaaggcag agatctactg tcacgtgggg ttcgggaagc
      241 tgaacgaaag taaactgtcc gatgaagcat cgaaatctta gatgatgcca aaatggggcc
      301 gtacagccgt cccctgataa gcgcagaaga cacctgctta ctgactgcgc ggcatccggt
      361 gtgaaggcag caggatcccg ggccagacca tctcagggaa cgtgagaacc tgtttccggc
      421 acgtatcggg aaacaggggc ggacccattc gtagtagtga tgaaagatct gtaatgggtc
      481 tggagcgaag ggatggggat cacattcatt gcagaagaca caacccaaaa ggggatgacg
      541 taactgatgc aacgaaaatc atttgaaata ccaaaagcac tggtctgggc atcatatctg
      601 gatgtgcgtc gcaacaaggg agccccccgg atgcgacggg cagactttga aaatgttcga
      661 ccagcaacgt gatggcaatc tatacaagat ctggaaccgg ctatgttcgg gaacatggtt
      721 tccgccacca gtgctggaaa aacggatccc gaagtcgaat ggcaaggaac gcattctggg
      781 gatcccaaca gtatccgatc gaattgctca gggagcgata aaacttttca tggaagaaaa
      841 gcttgacccg attttccatg ctgattcata tggttaccga ccaggcaaat cagctcatga
      901 cgctctgaaa caatgtgcca tccggtgctg gcgttatagc tggatactgg aagtggacat
      961 cagtgcgttt ttcgaccatg tgagacatga tctggtgctc aaagctctgg aacaccacgg
     1021 gatgcctaaa tgggtcatcc tgtactgtcg acgctggatg gaagcgccaa tgcagagttg
     1081 tgaaaatgga gaattaataa cccgaacacg aggcactccg cagggcgggg tcattagtcc
     1141 gctacttgct aacttgttcc ttcactatgc atttgatttg tggatggaaa gagaatatcg
     1201 gggggtaccg tttgagaggt acgctgacga tattgtagta cattgttcac gaatgagtga
     1261 tgtaacaagg ctaaagaaca ggttatcgga gcgcttctcg gaagtcgggc tggtcctgaa
     1321 tgcagggaaa acgaatatcg cctacattga cacgtttaaa aggagaaacg tcgcaacgag
     1381 tttcaccttt ctcggatatg acttcaaagt gcgtacgctg aagaatttca aaggcgaacg
     1441 gtaccgaaaa tgcatgccgg gtgcgtcaaa tgcagcaatg cgcaaaataa cagaaacaat
     1501 caagaagtgg cgtatacatc gctcaacagc tgagagtttg ctggattttg cgaggcgcta
     1561 caatgcgata gtgagaggct ggatcgagta ctacggaaag ttctggtcca gaaatttcaa
     1621 ctatcgactg tggagtgcaa tgcagtcacg tctgctcaag tggatgcagt ctaaatacag
     1681 actttcgaac cggaaggctc agcgaaagct gacgctggta aggaaggagt atccgaagct
     1741 atttgttcac tggtatttac tacgtgcatc aaatgagtga tcaagagccg tatgacgcga
     1801 gagtgtcacg tacggttccg tgagcagccg gggagtgaaa ttctctccgg ctgactcgac
     1861 tcgcgggttc cgacagcgat gacgaacatg cggcggtgtt gtactcgctg atcggcacat
     1921 gccgtctgaa caatgtggaa ccagaaaaat ggctgcgtta cgtcattgag catatccagg
     1981 actggccggc aaaccgggta cgtgatctgt tgccctggaa agttgatctg agctctcagt
     2041 aaatatcaat acggttctga cgagccgctt acttttggcg taattctgct gcgcctttac
     2101 tgaacacaat gccgtattca gcaagaagtc cacgaatgtg atttatcagt tgtgtccttt
     2161 ccattgaaac caaccgacgc atattcaaaa aagtctggac gtcctgttgc tccagagttt
     2221 ttattggtac gggtcgtata gttccatgtt ggcaggcctc agctattgcc tgtgcgtcgt
     2281 tataatcatt tttctggcac cgctgataag ctttaacatg ttgtgccgga agcaatatta
     2341 catcatggtc gagtttcctg atttcccggg cccagtaatg agatgccccg ccagcctcca
     2401 tcgcaacagt cattttaggc tgttgcacct gccagttaag cagttgattg cgagaaagtt
     2461 tggtctttcc tgcgggtttg ccatgagcat tgatactgaa aagataaaag ttcgttttag
     2521 ccagatcaat gccggtgatt ttctgttcag acatggtatc ccgcttactt ttgtggttaa
     2581 agtttatcca tcatggcgca attgaaggct gccgtgaaag agggggaaga ctatctcatc
     2641 ggttactgtt aaacggctaa gagaaactat ctttttcaag caaagaagat acggtgaaac
     2701 aggcgatttc accgtgaaat actgttcaaa aaaaacgcta ataaaagcaa aatttctttt
     2761 aaaacagcca attaaaaact ggtgagttgc tggcgaaaaa cttgaatttt cacagcgaat
     2821 tcagattatc ttttagtaca agagcaatca tacgatctaa cttatcctga acttccaacg
     2881 gtaatctgcc gaattcatat gagaacgtac gtccctttac tcgttttcga gcaaatttat
     2941 ctttactatc aaatttccat aacaaagtta caaccgcttt atcctttact cctgaatcaa
     3001 gcaatgaaga catttccttg gttataagcc gcataatact gtttttaacc tcgtcttcag
     3061 aaagattgtc atcagcagta ataagctcaa tactcggcga gatattcttc acaacttcat
     3121 caatagatat atcacccagc ctcagtcgtt cacttaattc agccagttgc ctgtaatcgg
     3181 cgtatgtcag ctcagactga acaggaaaaa gagacacaaa atctttaggt acggacgctg
     3241 cttggagggc acgagtcacc ttagctgccg acaatccctc tctttccgca atttgcttct
     3301 ggttcatacc ggcctctttg agacgtacta aacgcaaccc aatttcacga atattatgtt
     3361 caagtgacgt ctgcaaatct ttcgccagat gctgagcttc agaaacagta agctcatcat
     3421 ccgttacaag tacacgtaat ccaaccttgc atagcaaagc tgctgcccga cgacgggaac
     3481 catccaaaat ctcaataaga tcgccagtcc gaatcccaat acatggatag aattgttgta
     3541 aacgtatagt tctggtgatg tcttttagtg agtccggcgt cagggctgtc tgttcgcggc
     3601 cattcacctc ttcaaccaca aaagtttgag tgtccacttt gtcatgatca accttgatct
     3661 cagtaaattt tgcctttcgg ccagtattga gggtaaatac atgaactgga gccgatattt
     3721 cttcagtatt attcaggata tttgtattca gcgtacggcc aatagtcggt cggtgctttc
     3781 tgttttccat cagtagttgg cccttataaa ttcaatacga tcaaaaactg ctttagcgaa
     3841 atcttctgct gccatacgtg catttttcaa agcttcacca ctgccaacat aagtaacagg
     3901 attcgcagat atcacggtat caaacgactc gccgcttctc tcaaaaccat caagacgtgg
     3961 cattgaaaca tcaagcatgt caccaccgaa aatctctttt gtcaggctat ggcagtattt
     4021 atgatctgac ttattcgcca gtttcgacat gaagccaata tttgcctgca aacggcatga
     4081 acatccatcc tgttcaataa tctgtacaag ttcaggtaaa cgagccaaat atttaagcgt
     4141 ggaatggaaa tcaacttgtg ctggcggcac cggtgtaaac atgatatctg cggcagcaat
     4201 ggcatttttc aaaaatgcgt ccagatgcgg gcctgtatca ataaggatga agtcgaaatc
     4261 atgcttcagc ttatcaataa tattctcacg caatatcgcg tgtttgtttt gtcctaatag
     4321 atgctcctcg cacagagtat cccagtttga tgcaataaag gcgtcgtcaa tagaagccgg
     4381 cataacataa acgccaggga taactgacgg aacgataaaa tcttccagta attcctcacg
     4441 actcacgttc tgcaacatag cctgcggtgc ggttgtatca accagcccca ccgcatgcaa
     4501 gtagttcaga aacatagtgg ctgatgactg gggatctaaa tcaaggagca gaatacgtaa
     4561 atcttctgac agcaggtgag gatgagcacg cagggcgtga gctacggaaa cactactcac
     4621 ggttttagac acaccgcctt ttagactacc gatgaatatg gaatatgcct cactataacg
     4681 atcgcgatat ttaggaatgc cacgatgtgc atagatatcg ataatgttct ggatggttaa
     4741 tgcatatttt gtcgcggttc ctgctgggcg tttttcaaac tgatagccct gagcctccat
     4801 ctcaccgact gcaccatcca cgcttgcgcg cgttaatttt ggaagcttag ctacagcaga
     4861 ctttgaatag acttgataaa aaacatcagc ctgaaactcc tgcttttgct cctgaatctg
     4921 ttttgttagg gcgagcaaca ttttatctgc acgctgcgca accttactta actgctcaaa
     4981 actagtcatt ccccctccgt gctatgtagg attttaactt attacaaaaa aaactgcatt
     5041 agcagggtaa tgcatgcaag tacatattgt acatgcttat attaaaaatg cgcgcattgc
     5101 tgatttatta atcatcggaa tgaatattac tgtaagatag tatgcctttc cgggagaaag
     5161 acgctgattt cccccgaata atccagttca ccaccacact ccggacaaca gctttcttca
     5221 ggctccagat ggtgtatttc gcgggaaaca tgttccggta acggacggcg atgacgtgac
     5281 tgtcgcaaca ggcgggggta gtgcttccaa tcagtagaac atgtgctttt caatgaagga
     5341 gccgatgact ttttcgtgga tttccacgga acgcgagaag cagattgttt tacgtgccag
     5401 ccgcttgatg cgggtgcaga gggtccggtt gttacgttca atacgttgcg tgaagatttt
     5461 gccggtcagg tgtttctcct tcggcacttc tctggcgtag ctgccccagt catcgctggt
     5521 caccatgccg atacagaacg gagtcaacag ggctaacagt tcccgacagg tctcatcatt
     5581 ccgtggcccg aaagtgtaag ccaacacacc gccagtttta gtgttgtacg cataccagag
     5641 ccagtgttgt gtcaacgacg gatgaaaagt gctccactta tatctccacc aacggcccaa
     5701 tattgatcca ccgttttact caggattagc ttctgctata accccggcct ttcgtttctg
     5761 tctgagtcga tagctttctc ctttgatttg aacgacatgt gagtggtgta agatacggcc
     5821 cagcatcgct gaggtcagtg ctgcatcacc ggcgaacgtt tgatcccact gcccgaacgg
     5881 tagattggat gtcaggatca ttgcgctctt ttcgtaacgt ttagcgatga cctggaagaa
     5941 cagttttgct tcttcctgac tgaacggcag atagcctatt tcatcaatga tgagcaggcg
     6001 ggggccatta ctccacgctg aagcgtcgtt ttataacggc cctgacgttg tgccgtagat
     6061 aactgaagta acagatctgc tgctgttgtg aagcgaactt tgatacctgc acggactgct
     6121 tcatagccca tcgcta
//
LOCUS       DAJTXY010000177         6036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTXY010000177 DAJTXY010000000
VERSION     DAJTXY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.179"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(127..276)
                     /locus_tag="OO566_003848"
     CDS             complement(127..276)
                     /locus_tag="OO566_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606934.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            1465..2280
                     /gene="sul2"
                     /locus_tag="OO566_003849"
     CDS             1465..2280
                     /gene="sul2"
                     /locus_tag="OO566_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6606935.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2367..2669
                     /locus_tag="OO566_003850"
     CDS             2367..2669
                     /locus_tag="OO566_003850"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606936.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2845..4338)
                     /locus_tag="OO566_003851"
     CDS             complement(2845..4338)
                     /locus_tag="OO566_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCR6606937.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(4382..4771)
                     /locus_tag="OO566_003852"
     CDS             complement(4382..4771)
                     /locus_tag="OO566_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606938.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4989..5429
                     /locus_tag="OO566_003853"
     CDS             4989..5429
                     /locus_tag="OO566_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6606939.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1434 a         1664 c         1583 g         1355 t
ORIGIN      
        1 aaaaaaaagg aatatttcgt tttcacgagt aatacccggg cattaaaaat ccactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt
      181 ctcttcagta acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaatcc agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag
      301 gttgttagcg ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat
      361 actggcttac tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga
      421 aaaaaggcag caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg
      481 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg
      541 ggacggaaat ttcctagaag ataccaggaa gacacttagc aaagaagaga taaggccacg
      601 gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca
      661 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
      721 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      781 ccgcggtttc tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg
      841 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa
      901 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg
      961 agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga
     1021 aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca
     1081 actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga
     1141 gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc
     1201 ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc
     1261 tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag
     1321 agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa
     1381 acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg
     1441 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1501 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1561 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1621 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1681 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1741 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     1801 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     1861 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     1921 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     1981 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2041 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2101 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2161 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2221 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2281 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
     2341 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
     2401 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     2461 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     2521 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     2581 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     2641 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     2701 ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca ctagcggctc
     2761 cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca gcattttgat
     2821 tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct cagccggtgg
     2881 cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct tcaggtgatc
     2941 aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca tggcgccgcc
     3001 gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt gcgcccatgt
     3061 catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga ccttgttgcc
     3121 cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg cgaacacccc
     3181 gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc ttgcaatgaa
     3241 atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct tgagctggta
     3301 gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg ggcggctgat
     3361 gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca cgccggcgtg
     3421 caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac tggtcggcag
     3481 agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac ggtaagtgat
     3541 cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg ccagatagct
     3601 gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca cccggtgggc
     3661 gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc agcggaaacg
     3721 cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt ggaagtgaac
     3781 attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg tcttggccac
     3841 ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt aaacgatgcc
     3901 cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct gaaacgggaa
     3961 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     4021 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     4081 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     4141 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     4201 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     4261 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     4321 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     4381 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
     4441 cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg ttattgcgtg
     4501 caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa ttaatattct
     4561 tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat gcagtataag
     4621 atgaaccact actattatca tggccaccat taccaccacc gttaaaacta cctcccgaac
     4681 ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac
     4741 cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact tttaaaaaca
     4801 gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact acgcccgtca
     4861 aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca gcctcagacc
     4921 ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca ggagacactg
     4981 tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga aagtcgcgct
     5041 cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc
     5101 gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc acttctgaga
     5161 caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg gaaaatgctc
     5221 agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc ctaatgcttg
     5281 aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac
     5341 aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg ggattctggt
     5401 cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt accataacat
     5461 ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac cggtaaaaca
     5521 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5581 gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg taagtcctgt
     5641 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5701 aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta
     5761 caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa aagtgatgta
     5821 aaagtgatgt atgaactgaa ggagcgatgt aattctgtag tcaggaagaa taacccgttc
     5881 atgcaggcat gaacgataca gtgaggaacg ctttttttgt cacgatctcc ccttaacctg
     5941 cgtaaatcac ccgccatggt tgattatttt tcaacctcaa aatggtcata cacctaacgc
     6001 agccgcgatt ttcatcgcaa ctgcatgaat taaaag
//
LOCUS       DAJTXY010000178         6009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTXY010000178 DAJTXY010000000
VERSION     DAJTXY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.180"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..337)
                     /locus_tag="OO566_003854"
     CDS             complement(<1..337)
                     /locus_tag="OO566_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="regulates several genes involved in high affinity
                     phosphate uptake; under conditions of high phosphate
                     concentrations downregulates the PHO regulon; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate transport system regulator PhoU"
                     /protein_id="HCR6606940.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRT"
     gene            complement(352..1125)
                     /gene="pstB"
                     /locus_tag="OO566_003855"
     CDS             complement(352..1125)
                     /gene="pstB"
                     /locus_tag="OO566_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6606941.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPELRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1217..2107)
                     /gene="pstA"
                     /locus_tag="OO566_003856"
     CDS             complement(1217..2107)
                     /gene="pstA"
                     /locus_tag="OO566_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6606942.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2107..3066)
                     /gene="pstC"
                     /locus_tag="OO566_003857"
     CDS             complement(2107..3066)
                     /gene="pstC"
                     /locus_tag="OO566_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6606943.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3153..4193)
                     /gene="pstS"
                     /locus_tag="OO566_003858"
     CDS             complement(3153..4193)
                     /gene="pstS"
                     /locus_tag="OO566_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6606944.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLEKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4441..5514)
                     /locus_tag="OO566_003859"
     CDS             complement(4441..5514)
                     /locus_tag="OO566_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6606945.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSSLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTYDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(5525..>6009)
                     /locus_tag="OO566_003860"
     CDS             complement(5525..>6009)
                     /locus_tag="OO566_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6606946.1"
                     /translation="APGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFS
                     KSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASL
                     ADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH
                     "
BASE COUNT         1526 a         1559 c         1423 g         1501 t
ORIGIN      
        1 cagtacggca gattttgtcc gccacgtcgc caatacgctc cagctcggca atggttttac
       61 tgatcaccat aaccagtcgc aggtcgctcg ccgtcggctg acgtttggcg ataatgcgca
      121 cgcaggcttc atcgatcgcc acttccatca tgttgacgtt cttgtcgcct tcgatcacgc
      181 gcttcgccag atcgctgtcc tggttatgca tcgcggtgat tgcatcagaa agctgctgct
      241 ccaccatgcc gcccatggtc atcacctgcg tgcggatact ttccagttcg gcgttgaact
      301 ggccggaaat atgtttatta agattgagac tgtccataac gcactcctga atcaaccgta
      361 acgaccggtg atgtagtctt ctgtttgttt cttcgctggc ttggtgaaca gatcgtccgt
      421 gttgctgaac tcaatcaatt cgcccaggta cataaatgcc gtgtggtcgg aacaacgcgc
      481 agcctgctgc atgttgtggg taacgatcac cacggtgtaa tcctgcttca gttcggtgat
      541 cagctcttca atacgcccgg tagagatggg gtcgagcgcc gaacacggtt cgtcgagcag
      601 cagcacttcc gggcgaatgg cgataccacg cgcaatacac agacgctgtt gctgaccacc
      661 agagagagag taaccgctct ggtgcaattt atctttggtt tcgttccaca atgcggcttt
      721 ggtcaatgcc cactgcacgc gctcgtccat gtcggcacgg gagagcttct caaacagacg
      781 aacgccaaag gcgatgttgt cgtagatgga catcgggaac ggcgtcggtt tctggaacac
      841 catgcccact ttcgcacgta gcagggcgat atcctgagag ttggtcagga tgttgtcgcc
      901 atcaagcaga atttcacctt ccgcacgcag ctccgggtac agttcaaaca ttttgttgaa
      961 ggtacgcagc agcgtcgatt taccgcagcc ggacggcccg ataaacgccg taacctggtt
     1021 tttggcgata tccaggttga tgtttttcag ggcatggaat ttgccgtagt agaagttcaa
     1081 attacgaacc tgaattttac tcggggcagt ttcaaccata ctcattgcaa tcttttcctc
     1141 atcgtacctg atgcttttcg ctaatcaggc ttaccgtagg gccgggtaag gcgttcacgc
     1201 cgcatccggc aaagtgttaa ccgtgtttat tcttcgcaaa aacaacgcgc gccagaatgt
     1261 tcagcagcag gacgcacagg gtaatgatca ataccccggc ccaggccagt tgttgccatt
     1321 ccgcaaacgg gctcatcgca aacttaaaga tcgtcaccgg caggttggcg atcggctgca
     1381 tcatgtccgt gctccagaac tggttggaga gcgcggtaaa cagcagcggc gcggtttcac
     1441 cggcaatacg ggcaatcgcc agcaggatac cggtcataat cccggacacc gacgctttca
     1501 gcgtaatcgc ggagatcatc ttccacttcg gtgtacccag cgcataagcc gcttcacgca
     1561 ggctgtccgg caccagtttc agcatgttct cggtggtgcg gataacgatc ggcacctgca
     1621 acagcgccag cgcaattacg cccgcccagc cggagaagtg ctccatctgt gccaccacga
     1681 tggtgtagac aaacagacca accacaatcg acggcgcgga gagcagaatg tcgttaatga
     1741 agcgaatcac ttctgccagc caggattttc gaccatattc cgccagataa atccccgcca
     1801 taatgcccag cggcgtaccg aataccgtgg cccacaaaat taacagcccg ctacccgcca
     1861 gggcgttcgc cagaccacca ccttccgtat tgggcggcgg cgtcatttca gtgaacagcg
     1921 ccagcgacat accgtcgata ccgcgagtga tggtggacat taaaatccag atcagccaga
     1981 acaggccgaa ggccatcgtc gccatcgaga gcgtcagcgc aatacggttt ttgaggcggc
     2041 gacgcgcctg cattttgcgg cgagattcag ccagcgccgc agtggtttgc atttcaacca
     2101 tagccattag cgtgccccct cattcttagc caggcgcata atcataaact tcgatgcggc
     2161 gaggacgatg aaggtaatca caaacaggat caggcccagt tccatcagtg cggcaacgtg
     2221 cagaccggat tccgcttccg caaattcgtt cgccagcgca gaggtgatac tattgcccgg
     2281 catgtacagc gaggcgctgt cgagctggta ggtgttaccg atgataaagg tcaccgccat
     2341 ggtttcaccg agcgcgcggc ccagccccag catgatgccg ccgataacac catttttggt
     2401 gaacggaaga acgatacgcc agataacttc ccaggtggtg cagccaatac cgtaggccga
     2461 ctctttcatc atcaccgggg tttgttcgaa cacatcacgc attaccgccg caatgtacgg
     2521 aataatcatg atggcgagga tcacgcctgc cgcgaggata ccgataccaa atgcggggcc
     2581 agagaacagc gcgccaacaa tcgggatatt cgacatgata ttgccgaccg gctcctgaaa
     2641 gtaaacggcg aacagcggcg caaagataaa caggccccac atgccgtaaa cgatacttgg
     2701 aatggctgcc agcagctcaa tggcgatacc cagcgggcgt ttcagccagc caggcgcaag
     2761 ctcagtcagg aacagggcga taccgaaact caccgggacg gcgatcagca gcgcgataaa
     2821 cgaagtcacc aacgtaccgt agatcggcac cagcgccccg tagatatcgt tcggtgcatc
     2881 ccactctttg gtccatagga aagccagacc aaatttctga atgctcggcc aggaggagat
     2941 gatcagagag acaataatgc cacccaacat caatagcaca atcagcgccg ccagttttac
     3001 cagcacgctg aaaattatgt cgcccttttt acccggtggg ttaaaagcgg gcttggttgc
     3061 agccataagt tactcttcag ttaaacgcgt ttacaaagtc attttgtaat gccggatgcg
     3121 gcgtaaaaca ccgtacccgg cctggagttt tattagtaca gcggcttacc gctactgtct
     3181 ttaatattgg tcttccacgc agcgcgaacc tgttcaacta cactatccgg caggctagcg
     3241 taatccaggt cgttcgcctg tttagccccg gttttgtacg cccagtcgaa gaatttcagc
     3301 acctctgtgc cttgttctgg tttcttctga tctttgtgga tcagaatgaa cgtggtagag
     3361 gtaataggcc atgcatcttc gcctttctgg ttggtcagat cctgagcgaa ggttttgctc
     3421 cagtctgcac cttttgctgc attagcgaag ttttcttcgg tcggactaac cggtttacca
     3481 tcagcggaga tcagtttggt gtacgccagg ttgttctgct tcgcgtaagc atattcaaca
     3541 taaccaattg cacccggcag acgctgaacg aacgcggcga taccgtcgtt acctttaccg
     3601 cccagaccga tcggccattt tacggtagag ccagtaccaa cgttgttttt ccactcttcg
     3661 ttcactttct ccaggtagct ggtgaagacg aaggaagtcc cggagccatc tgcgcggcgt
     3721 actacagcaa tgttttgtga aggcagtttc agacccggat tcagtttggc gatggcttca
     3781 tcatcccact tcttgatttt gcccaggtag atgtcgccga gggttttacc atccagcacc
     3841 agttcgccag acttcagccc tggaatgtta accgccagca ccacgccgcc aatcacggtc
     3901 gggaactgga acagaccttc ctgagccagt ttttcgtcag acagcggcgc gtcagaggca
     3961 ccaaaatcaa cggtattagc gataatctgt tttacgccac cggaagaacc gataccctgg
     4021 tagttaactt tattaccggt ttctttctgg taagtgtcag cccatttggc atacaccggc
     4081 gcagggaagg ttgcacctgc acctgtcagg cttgcttctg caaacacaga gaaagcactc
     4141 atcgataagg tcgcggcgac aacagttgcg acggtggtac gcataacttt cataatgtct
     4201 cctgggagga ttcataaagc attgtttgtt ggctacgaga agcaaaatag gacaaacagg
     4261 tgacagttat atataaggaa tatgacattt ttatgacaga aaggaattac caaataactt
     4321 actgatttta aatgtgaaga tgaaaatccc acatgccatc tttttgtcat gatgcattta
     4381 aaagtgataa agaaatgggt gagatgacaa cgatgtgatg aacaatcgtt gtcagaccta
     4441 ttagcgcaca atctcaacag ttatcgtcgc tgttgcagta aattgccccg gggctgggcg
     4501 agctccagta aggctcgcag gagcagctga aaaagttaca ctacctgatt tttcaccata
     4561 cactgttgat ttatccatgt ctgttggcaa ctcaccaccg ttcactttga tttccttatt
     4621 atttttgtcg taaattttta tccccacgtt agagtttgaa gttacgacca ttgaattgtc
     4681 cgtattgggt tcggtagcat aaaaagacgt actgatatca taggtattca gtatccctag
     4741 gttttcacac tcaatagtga ctgttttagt aattttatgt gttgttaccg cacttccaac
     4801 aacagatgaa aaatctgcag ctggtatttc tttaaaatca aaacaaactg gctttaaatt
     4861 atttatttcg caactttgcg gagctgtaat atcgccccga atataaacat cagccatcgg
     4921 ctgtgagtaa tccaccgggg tgtcacggct aattgttcca tacaatgtca ctatctttgt
     4981 ttcaggaata atcaattttc ctgcaaatgt cttcttgata taaaaatata ctatcgcttt
     5041 agccccactg gatatactta aaggttcaat gcggttgcaa ttgtactttg tattaccaga
     5101 cgggttgtta gcatgatatt caaaaggtgc attcacatac ccaaccccaa gaatatctat
     5161 agcaatccca acatcaaggt tcggattaag atcataataa ttaatattag agtgaacccc
     5221 agcataagtt aatgcctggt ttatttttga cgtataatag atatagggga attctcctac
     5281 gttaccagta tcacaatgac agcgtccaag atatgaggat ccattagtta cagtggtgtt
     5341 aaatgtcgtt ccaaccttat tttcgttcgc ggtaataaga tatcccgaga aatcaagcga
     5401 actgtgaaat acgccattat caggggtaca gataccttta ggagctgcaa gtgctacact
     5461 acttaacgag agcataaaca cagaaaagca ccactgtttg atatacttgt tcataaaaat
     5521 ccttttaatg acatgtcaca ccctgttcaa ttaatccgct ctcactttct ggtgtcgaaa
     5581 gtgaataatt gaagatgcat tttgttgagg cgttttctcc ccaggataac gtaacctgtc
     5641 ctttttctgg tagaccagaa agataaagtt cgccacggtc accgacaatt cctgaatctg
     5701 ccaaacttgc atcttgtatc gcagcaatgg ctccaaaagg taccggttta ccatttgcat
     5761 ttaacactct gaagagaacg cgataaccca catgagtcac aaactccgct ttaaccactg
     5821 caccacgcgt gggaaccatc gtaacgacgg tattatcaag ttctatattc gacttcgaga
     5881 aatagttatc gctgattgct acccgatttt catcataagg gtttagctgt gtcttgaccg
     5941 tatagccgcg ccagtccgtc gcagcaccgc gcatattatc tatttctaac ccggcggccc
     6001 caggtgctt
//
LOCUS       DAJTXY010000179         5976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTXY010000179 DAJTXY010000000
VERSION     DAJTXY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.181"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..111)
                     /locus_tag="OO566_003861"
     CDS             complement(<1..111)
                     /locus_tag="OO566_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HCR6606947.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGT"
     gene            complement(368..649)
                     /locus_tag="OO566_003862"
     CDS             complement(368..649)
                     /locus_tag="OO566_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6606948.1"
                     /translation="MWGVELLAIRYAAWIKPEFEIEVYEVFKTVVRLGVGAMSRLNRI
                     DHIINTETKAISQCASQMAKWGVGGRKRLLHVARERAANEVQMYLPGMV"
     gene            complement(673..873)
                     /locus_tag="OO566_003863"
     CDS             complement(673..873)
                     /locus_tag="OO566_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6606949.1"
                     /translation="MKYPTVIVNGVSVRVDEDGRYNLNDLHAAAVANGEATEQQRPSQ
                     FLRSAQIKRFIKALWNKFNHLK"
     gene            complement(1079..1231)
                     /locus_tag="OO566_003864"
     CDS             complement(1079..1231)
                     /locus_tag="OO566_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606950.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSKSRLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(1219..1686)
                     /locus_tag="OO566_003865"
     CDS             complement(1219..1686)
                     /locus_tag="OO566_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6606951.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(1683..2159)
                     /locus_tag="OO566_003866"
     CDS             complement(1683..2159)
                     /locus_tag="OO566_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR6606952.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
     gene            complement(2143..2466)
                     /locus_tag="OO566_003867"
     CDS             complement(2143..2466)
                     /locus_tag="OO566_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR6606953.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            2691..2909
                     /locus_tag="OO566_003868"
     CDS             2691..2909
                     /locus_tag="OO566_003868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606954.1"
                     /translation="MATAQFGLWRPVLISGLLQLPTAGYVATPNPAKDSCPYTSACAF
                     TTTERALANQARRLFTIIGSMLLPVVQI"
     gene            complement(3178..3801)
                     /locus_tag="OO566_003869"
     CDS             complement(3178..3801)
                     /locus_tag="OO566_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6606955.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTELWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(3798..3986)
                     /locus_tag="OO566_003870"
     CDS             complement(3798..3986)
                     /locus_tag="OO566_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR6606956.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(3983..4345)
                     /locus_tag="OO566_003871"
     CDS             complement(3983..4345)
                     /locus_tag="OO566_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6606957.1"
                     /translation="MSEYQFVLPYPPSVNTYWRRRGNQYYISDKGQKYRKEVQQIILQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(4342..4632)
                     /locus_tag="OO566_003872"
     CDS             complement(4342..4632)
                     /locus_tag="OO566_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6606958.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(4632..5354)
                     /locus_tag="OO566_003873"
     CDS             complement(4632..5354)
                     /locus_tag="OO566_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCR6606959.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEDCGRINGLGLNQSFCVYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(5347..5556)
                     /locus_tag="OO566_003874"
     CDS             complement(5347..5556)
                     /locus_tag="OO566_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCR6606960.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDE"
     gene            complement(5516..5917)
                     /locus_tag="OO566_003875"
     CDS             complement(5516..5917)
                     /locus_tag="OO566_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606961.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIQLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANTTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
BASE COUNT         1360 a         1534 c         1357 g         1725 t
ORIGIN      
        1 agtgccaatg aactgaaatg cttcttctgc ggctgcgttc tggttcataa ccagtttgta
       61 aatctctaac tggaatttct gttcttcagt catgggaata atctctgcca ttgttggctc
      121 cgtttatccg ttaaaaggga tatcagttaa gttatcccgt gtagggtata agccattatc
      181 gagaccactc attgaatggc ctctgcaata accgatgtct ttccatcagt ccgccaccac
      241 aaagaatctt ttttgccata aggctggagg ttcatctttc agtggctgcc agtgttattt
      301 ccccacttac tggcttgggt tgtttcgcgg tactgccgta atgcaaaaac tggattaacc
      361 tgcgaaatca caccattccg ggcaaataca tttgcacttc atttgccgct ctctcacgtg
      421 caacatgaag caatcttttt cgcccaccaa cgccccactt agccatttgg cttgcgcact
      481 ggcttatcgc tttggtttca gtattgatga tgtgatcgat tctattcaga cgggacattg
      541 cgccaacgcc gagacggaca accgttttga aaacttcata aacttcgatt tcaaattccg
      601 gcttaatcca tgctgcatat ctgattgcca gaagttcaac accccacaca cctggttctg
      661 caccaccttt gattatttta agtggttgaa tttgttccaa agtgctttta tgaagcgttt
      721 tatctgcgcg ctacgcaaaa actggcttgg gcgctgttgc tctgtagcct ctccatttgc
      781 aactgctgct gcatggagat cgtttaagtt gtagcgtcca tcctcatcaa cacgaacgga
      841 cacaccattg acaataactg ttgggtactt catcagtgat taccttttag tgatgaacct
      901 tgtcacacag gattccggcc cacagaaagg caccgattac caaaccggca tcctcaaggg
      961 tcatcctgaa aggctctgtg ttcataagtc gcgcgtgtga agcgcgtttg ttgcagatat
     1021 aaaaaagccc cgcgaatgcg aggctaaatc ctggtgtttg tgatgactgg ctcttatctc
     1081 aacgcagccc cttaccgcgc gccagatgct caatatcaag catcagcaat gagatgttta
     1141 atctggattt actccagaag tgatcacccc cctgtctaca gagccagatg tgaaggatga
     1201 tgagtaaaat tatcgctatc atcgaaggca ttgcgtcctg atgtattcct gaagcgttct
     1261 cagtgctgtt tggtcgcgga taattccgtc ccggataccg agaacgtttc gtccagcaac
     1321 tggagagagt tcgacggtgg catcattgcc catgccggag gcgctggagg tttcggctga
     1381 ggatggcaca gggcattttc ctttgacgag caccctgcca ccattatcaa gcttgcgccg
     1441 aagagcatca ttttcagctt tcgcatcagc taactccttc gtgtatttag catcgagtgc
     1501 atcagcagca cgctggcgtt gctgcatgtc agtaatggtg gcggtcgcct gcttcagctc
     1561 actgactttt ttatctcgct gctctttgta ggtcatggcg ttatcacggt aatgattaac
     1621 cgcccatgac aggcagacga tgatgcagat aaccagagcg gagataatcg cggtgactct
     1681 gctcatacat caatctctct gaccgttccg cctgcttctt tgaattttgc aatcaggctg
     1741 tcagccttat gctcgaactg accataacca gcgcccggaa gtgaagccca gatattgctg
     1801 caacggtcaa ttgcctgacg aatatcaccg cgatcaatca tcggtaaagc gccacgttct
     1861 ttaatctgtt gcagtgccac agcgtcctgg cttttcggag agaagtcttt caggccaagc
     1921 tgcttacggt aagcatccca ccaacgggaa agaagctgat aacgtccggc ggctgttgat
     1981 tttagttttg ggtttagtgt gacaagtttg cgaggatgat cggagtaatc agtaaatagc
     2041 tctccgccta caatgacgtc ataaccatga tttctggttt tctgacgtcc gttatcagtt
     2101 ccctctgacc acgccagcat atcgaggaac gccttacgtt gattattgat ttccaccatc
     2161 ttctactccg gcttttttag cagcgaagcg tttgataagc gaaccaatcg agtcagtacc
     2221 gatgtagccg atgaacacgc tcgttatata agcgagattg ctacttagtc cggcgaagtc
     2281 gagaaggtca cgaatgaacc aggcgatgat ggcgcacatc gttgcgtcga ttactgtttt
     2341 tgtaaacgca ccgccattat atctgccgcg aaggtacgcc attgcaaacg caaggattgc
     2401 cccgatgcct tgttcctttg ccgcgagaat ggcggctaac aggtcatgtt tttctggcat
     2461 cttcatgtct tacccccaat aaggggattt gctctattta attaggaata aggtcgatta
     2521 ctgatagaac aaatccaggc tactgtgttt agtaatcaga tttgttcgtg accgatatgc
     2581 acgggcaaaa cggcattagg ttgttagcgc aacctcatgc cgcccacttt cacgaagatc
     2641 atgtgtagaa ggccgcagcg taactatcac tgatgaattc aggatagtca gtggctacgg
     2701 ctcagtttgg attgtggcga ccggtgctga tctccggttt gctgcaactg cctacagctg
     2761 gctacgtggc cacaccgaat ccagcgaaag attcttgccc ttacacatca gcctgtgcat
     2821 tcaccacaac ggaaagagca ctggctaacc aggctcgccg actcttcacg attatcggct
     2881 caatgctctt acctgttgtg cagatataaa aaatcccgaa accgttatgc aggctctaac
     2941 tattacctgc gaactgtttc gggattgcat tttgcagacc tctcagcctg cgatggttgg
     3001 agttccagac gatacgtcga agtgaccaac taggcggaat cggtagtaag cgccgcctct
     3061 tttcatctca ctaccacaac gagcgaatta acccatcgtt gggtcaaatt tacccaactt
     3121 tattcaataa gtcaatatca tgccgttaat atgttgccat ccgtggcaat catgctgcta
     3181 acgtgtgacc gcattcaaaa tattgtctgc gattgactct tccttgtggc attgcaccac
     3241 cagagcgtca tacagcggct taacagtgcg tgaccatgtg ggttgggtaa ggttggggat
     3301 tagcatcgtt acagcgcgat atgcggcgct tgctggcatc cttgaatagc cgacgccttt
     3361 gcatcttcca cactctttct caacaactct cccccacagc tctgttttgg caatatcaac
     3421 cgcacggcct gtaccgtggc aatctctgca tcttgcgccc ggcgtcgcgg cactacggca
     3481 ataatccgca taagcgaatg ttgcgagcac ttgcagtacc tttgccttag tatttccttc
     3541 aagctttgca acgccacggt atttccccga taccttgtgt gcaaattgca tcagatagtt
     3601 gatagccttt tgtttgtcgt tctggctgag ttcgtgctta ccacagaatg cagccattcc
     3661 gaatccggct tgtgattgcg ccatccccat agcagccatc acatcagtac cggaaagaga
     3721 gtcagaagcc gtggcccgtg gtgagtcgct catcatcggg ctttttggcg aatgaaattt
     3781 agctacgctt tcgagtctca tcgtcttccc ctcttgccct gtttgaccat caggacgccg
     3841 ttaactatta cgtgacgctc gcctttgctg tctcggttgt acttgagcac tgttcctctt
     3901 gcgcaggaaa gcatcctcgc cacttcggtc tgattgcctc gtgtctggat aagaagctct
     3961 ggtatcgttt gaattgtggc gttcatacgt tctccagttc ggtgattttt attccaagcc
     4021 ttccgcctgg tactttcaca ccacgaatta cgcgaatgtc atcgaattgc tcgtcgtctt
     4081 ccgcaaatcc ggcgtggata agggagtcga gtaaaccttt caggatgtta tcgaggtcgc
     4141 ggcggcggga gtctggaacg tctgcgatga ctttgatgcg gagtcgtgat ttggtgaaaa
     4201 tgtctaactt gagttggagg attatttgct gaacttcttt tcggtatttc tggcctttat
     4261 cgctgatgta gtattggttt ccccgtcttc gccagtaggt gttcaccgac ggcgggtatg
     4321 gaagcacaaa ctgatattcg ctcatgactt aatcttcccc tccttcagca gtatcgcctg
     4381 cgtcctgatc acgccttcga ggtggtaaag tctggcgtct ttgttgtcga gattatgggt
     4441 gcgtcggtcg atttcatcgt gacacgcgct acaagcccat gcgccgatca ggtcgtcagg
     4501 cttcattccc gttccgcaaa ttccagccat ccggtaatgt gccagaactg tagtttcagg
     4561 attaccattg catacgccgt aaatacgtac ctggcattct ctgccgcgtg cttctttgcg
     4621 tagattagcc attaagcagc ctcccctgtt actttcagca ttccattatc gagcagcttt
     4681 ctggtcagcc actgttgacc acgcccggtg atttttgtgg tgaacgatat ctgtattccg
     4741 tgatttgtgt tgaccgctgt ttctttcact gtgaaatagc cgcgatccat atattcctgc
     4801 attggcacat tgcgccggga acctgaagca ataaggattt tgtgatcgcg catccacgca
     4861 aacagtttgt ttggaccaat tccaacaacc tttgcaaagt ttccaatcaa aattccgctg
     4921 gcctcgccaa cgcgatcggc aaactcaact ttaggtgcgg caattgcgag ctggttttcc
     4981 agttgcattt tctgctcagc aagatcagca gcaaggcgca acgcttccgg tagcgttttg
     5041 gggatattaa ccgcagcttc ttcaagctct cgccagcggt caacaaggcg agcggtgaat
     5101 tccggcgaca actgggctac aacgacaata ctgtctcgct ttccttgttc gccttcgaac
     5161 acatacacac aaaaactttg atttaagcct aacccattga ttcttccaca atcctcaatt
     5221 tgaggaagcc ggataacacc atttttagcc agcgtttcga tggtacgttt cacattgtca
     5281 tgacgcttac caaccaactc agcgatttca atgcttgtca ttttgatggc attgccattt
     5341 attaactcat tcatcgtctt cttcctcgta cattgagcta ttcggatcgc tcatcagttc
     5401 tgcgcagcag tgctcacaca cgtgaacttc cagcacatgc agcttctgac cgcaattagc
     5461 gcacgttaaa gctcgctcga cgctttcttt ctggtattga agggattggg atgggctaag
     5521 catggctttc accattaaaa agtcgcttgt aagcatcgat gtctcgtttt gcttcaccga
     5581 gctttcgtct taattccatg ttttctgatt caagcttttc catgtcttgt tggtatcgat
     5641 cgcggtgttc tttccatgct tttcgatacg ccttcatgta tgtcgtattg gcctttctct
     5701 ttgcctgacg aactgcgtgg tggtttttca caaaccagtc agggtcgtta aatgctgctc
     5761 tggcgcaggt ataccaataa tttgttgcct ccctgtttag ccaataaata ctgataaatg
     5821 gcaactgtat cgacaccatt tttcgttgag actctttctc gccaaacatg tgcccttttt
     5881 tgatgctaag gccaaatcca ggttgaatta aaagcattgt catttcctcg cacgatgaac
     5941 cgccccggga atcctggaga ctaaacttcc tgagaa
//
LOCUS       DAJTXY010000180         5923 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTXY010000180 DAJTXY010000000
VERSION     DAJTXY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.182"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..274)
                     /locus_tag="OO566_003876"
     CDS             complement(<1..274)
                     /locus_tag="OO566_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6606962.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPE"
     gene            811..2712
                     /gene="mtlA"
                     /locus_tag="OO566_003877"
     CDS             811..2712
                     /gene="mtlA"
                     /locus_tag="OO566_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6606963.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2807..3955
                     /gene="mtlD"
                     /locus_tag="OO566_003878"
     CDS             2807..3955
                     /gene="mtlD"
                     /locus_tag="OO566_003878"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6606964.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3955..4542
                     /gene="mtlR"
                     /locus_tag="OO566_003879"
     CDS             3955..4542
                     /gene="mtlR"
                     /locus_tag="OO566_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6606965.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4554..4763)
                     /locus_tag="OO566_003880"
     CDS             complement(4554..4763)
                     /locus_tag="OO566_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606966.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5047..5409
                     /locus_tag="OO566_003881"
     CDS             5047..5409
                     /locus_tag="OO566_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6606967.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1548 a         1443 c         1470 g         1462 t
ORIGIN      
        1 tttccggttc agtcgctttc tcctgcgacg caacatgtga ctgcataccc cagccacgct
       61 ccggttgata gagcgtcgcc cagatccaca gaaacggcca gataacatgc agagtaaaca
      121 ggctcaccca gcccgccgta tgaatagcgt cggcatgggg gtgattgcgc tttttggcaa
      181 tcagataagg gatgtcatgt atcgcgatga ttccataaaa aatcaccaga aagacgaaga
      241 tcagcactcc caacgcgaaa tagtttagaa acatatctgc ctcggattca cgtttatcag
      301 tgttgttttt tggctggcag ccagaaggga gtcaggctga tattttgaca ataatccggg
      361 ttcgcgattc tcgccataac accaaagaat aatttttaga ggtgatgagt tgcttagtta
      421 cataacgatt gtatgacgaa ggcataacat gctgtagatc acatcaggtg aacgccgtaa
      481 gaaaatatct tgtgattcag atcacaaaga ttcaacaaac catcaaaaca aaaatgtgac
      541 attactcaca tttaaatgcc atttttagcg aaaatcaccg ccttgtttct tttttacaca
      601 ggcgttttgt gatgagcgtc acgtcaatta cctctctacc ccctatattt atgtgactga
      661 tatcacacaa aagcccgtcg actggacagt taaccgattc agtgccagat ttcgcagtat
      721 ctacaaggtc cggctacctc tgccgccaca ttaacaaaaa acctcgggct tccagcctgc
      781 gcgacagcaa acataagaag gggtgttttt atgtcatccg atattaagat caaagtgcaa
      841 agctttggtc gtttcctcag caacatggtg atgccaaata tcggcgcgtt tatcgcgtgg
      901 ggtatcatca ccgcgttatt tattccaaca gggtggttac cgaacgagac gctggcgaag
      961 ctggtcgggc cgatgatcac ttatctccta ccgctgctga tcggttatac cggtggtaag
     1021 ctggtaggcg gcaaacgtgg cggcgtagtc ggtgccatta ccaccatggg cgttatcgtc
     1081 ggcgcagaca tgccgatgtt cctcggttct atgattgcag gtccgctggg cggctggtgc
     1141 attaagcact tcgaccgctg ggtagacggt aagatcaaat ccggttttga gatgctggtg
     1201 aataacttct ccgcaggcat catcgggatg atcctcgcta ttctggcatt cctcggcatt
     1261 ggcccgattg ttgaagccct gtccaaaatg ctggctgcgg gcgttaactt catggttgtc
     1321 catgacatgc tgccgctggc gtctatcttt gttgaaccgg cgaaaatcct gttcctcaac
     1381 aacgccatta accacggtat cttctcgccg ctgggtattc agcagtccca tgaactgggt
     1441 aaatcaatct tcttcctgat tgaagctaac ccaggtccag gtatgggcgt gctgctggcg
     1501 tacatgttct ttggtcgtgg tagcgctaaa cagtctgcgg gcggtgcggc aatcatccac
     1561 ttcctgggtg gtatccacga aatctacttc ccgtatgtgc tgatgaatcc gcgtctgatc
     1621 ctcgcagtca tcctcggcgg tatgactggc gtgttcacgc tgactatcct gggcggtggt
     1681 ctggtttctc cggcatctcc gggttctatc cttgctgtac tggcgatgac accaaaaggt
     1741 gcttatttcg ctaacatcgc gggtgtgtgt gcggcgatgg ctgtctcctt cgttgtctct
     1801 gctattttgc tgaaaaccag caaagtgaaa gaagaagatg atattgaagc agcaactcgt
     1861 cgtatgcagg acatgaaagc tgagtctaaa ggcacatctc cgctatctgc gggcaatgtg
     1921 actaacgacc tgagccacgt acgtaaaatc atcgttgcct gtgacgccgg tatgggttca
     1981 agtgcgatgg gcgcaggcgt gctgcgtaag aaaattcagg atgcaggtct gtcgcagatt
     2041 tcggtcacta acagcgcgat caacaacctg ccgccagatg tggacctcgt catcactcac
     2101 cgtgacctga ccgaacgcgc tatgcgccag gttccgcagg cacagcatat ttcgctgacc
     2161 aacttcctcg acagcggcct gtacaccagc ctaaccgaac gtctcgttgc tgcccagcgc
     2221 catactgaaa acgaagtgaa agtgaaagac agctttgacg attccagtgc taacctgttc
     2281 aaactgggcg cggagaacat cttcctcggt cgcaaagcag caaccaaaga agaagcgatt
     2341 cgttttgctg gcgagcagct ggtgaaaggc ggttacgttg agccggaata cgttcaggcg
     2401 atgctggatc gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacac
     2461 ggtacggttg aagcgaaaga tcgcgtactg aaaacgggcg tggtgttctg ccagtacccg
     2521 gaaggcgtgc gcttcggtga agaagaagat gacattgccc gtctggtgat tggtattgca
     2581 gcccgtaaca acgagcacat tcaggttatc accagcctga ccaatgcact ggatgatgag
     2641 tccgtcatcg agcgtctggc acacaccacc agcgtggatg aagtgctgga actgctggca
     2701 ggtcgtaagt aatccaatcc taccctctcc tcacggagag ggtttgggtg agggaaaagc
     2761 ctcaccccag ccctctcggg taaaaacatt gatgaaggtt aatactatga aagcattaca
     2821 ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag acgcgggtat
     2881 ccaactgacg tttgccgatg tcaatcaggt ggtacttgat gccctgaatg cccgtcatag
     2941 ctatcaggtt catgtggtcg gtgaaaccga gcaggtagat accgtttccg gcgtcaatgc
     3001 tgtcagcagc attggtgatg atgtcgttga tctgattgct caggtcgatt tagtcactac
     3061 cgccgttggc ccggttgtgc tggaacgtat tgctccggct atcgccaaag ggctggtgaa
     3121 acgcaaagaa caaggtaatg aatccccgct gaacatcatc gcctgtgaaa acatggtacg
     3181 cggcaccacg cagctgaaag gtcatgtgat gaacgccctg ccggaagacg ccaaagcgtg
     3241 ggtagaagag cacgttggct ttgtcgattc cgccgttgac cgcatcgtac cgccttcggc
     3301 ttcggcaact aacgatccgc tggaagtgac ggtagaaact ttcagcgaat ggattgtcga
     3361 taaaacgcag ttcaaaggcg cactgccgaa catcccaggc atggagttaa ccgacaacct
     3421 gatggcattt gtcgaacgta aactcttcac cctgaacacg ggtcatgcta taaccgcgta
     3481 cctcggaaaa ctggccggtc atcagaccat tcgtgacgct attctcgacg agaaaatccg
     3541 cgcggtggta aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc gctacggctt
     3601 tgacgcagac aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg agaacccgta
     3661 tctgaaagat gatgtagagc gcgtaggccg tcagccgctg cgtaaactga gtgctggcga
     3721 ccgtctgatc aagccactgc tcggtacgct ggaatacagc cttccgcaca agaatctgat
     3781 tcaggggatt gctggtgcaa tgcacttccg cagtgaagat gatccgcagg ctcaggaact
     3841 ggcagcactg atcgctgaca aaggtccgca ggcggcgctg gcacagattt ccggtcttga
     3901 tgccaacagc gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc aataatggtg
     3961 gaccaggcgc aggacaccct gcgcccgaat aacagattgt cagatatgca ggcaacaatg
     4021 gaacaaaccc aggcctttga aaaccgtgtg cttgagcgtc tgaatgctgg caaaaccgtg
     4081 cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct tctggtgctt
     4141 caggtattcc gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt actcgacggc
     4201 gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt gggcgtcatt
     4261 aaccgccagg aatacgaaga tgcggaactg ctgatggcat tgcgtgaaga gctaaatcac
     4321 gacggcaacg agtacgcctt taccgacgac gaaatccttg gaccctttgg tgaactgcac
     4381 tgcgtggcgg cgttaccacc gccgccacag tttgaaccag cagactccag tttgtatgca
     4441 atgcaaattc agcgctatca acaggctgtg cgatcaacaa tggtcctttc actgactgag
     4501 ctgatttcca aaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc tgcttactgc
     4561 ccctctacct gcttcggccg ataaagccga cgataatact ccagacgttg tagatataac
     4621 ggcacgcttt catcgggtat tccggacgga atcgcgttac ggggaggaag ttttttcaga
     4681 tactcccgga acgcctggct tgatgccatg aaatctacgg ctttatcgat aagaagcgga
     4741 acgttttcac ctaatttgcc catgatgata tctccgtatt accccgcgcc gggaatgcgc
     4801 ggccgccaat tttagtttag gttcccggta aagatgacat taaggaaaac gtgctgaatc
     4861 ctcaataatc atccgctgct gtttattatc tttttctata gatttcttaa ttaatcaacg
     4921 aattgcgtag tttttgagca tgttaatgat tacgttgcag aactattaca atgtcatcag
     4981 ttgtgaacaa agcacctggt cgcgcatact aggggctata aatttatctt tatcagaagc
     5041 catcacatga aagaagtcga aaaaaacgaa atcaaacgtc tcagcgatcg cctggacgcc
     5101 atccgccacc agcaggccga tctgtcgctg gttgaagccg cagacaaata tgccgagctg
     5161 gaaaaagaga aagccacgct ggaagcagaa attgctcgcc tgcgtgaggt tcatagccaa
     5221 aaactgagta aagaagcaca gaagctgatg aagatgccgt tccagcgagc aattaccaaa
     5281 aaagagcagg ctgatatggg caagctgaag aaaagtgttc gcggactggt ggttgtgcac
     5341 ccaatgaccg cgctggggcg cgaaatgggc ctggaagaga tgactggatt ttctaagact
     5401 acgttttaag cccctcagac gactggttcg atcgtatcaa cccgcatctg gcttgcctta
     5461 gctggtcaat gcggggactc tcctgtctac tcgcctaatt accaaacaat tcactccctt
     5521 tacttcgatt taccgctggt tgatattctg aaaatgcatt tttaccgaaa tagccatcta
     5581 taaacaataa aaatatatag aagatatcat ttgttaatta acaagcattt acgtctcttt
     5641 ttttcggatt taattgtgaa attttaatca tcatattcac aaaatcaatt atctgtaaaa
     5701 tttttcccag acatggtttc tttcatattt gacatttatt aataatcatc attagaatcc
     5761 acgaaaaata atatattcat acaaacacag acatcttttg aaacgaatga tacattttta
     5821 tgatgaatgc ttaaaacaat atttaatcga cacacatcac tgtcattaat gtaaagcaac
     5881 atagctcacc aataatacaa tggacaactc taccggcatt gat
//
LOCUS       DAJTXY010000181         5846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTXY010000181 DAJTXY010000000
VERSION     DAJTXY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.183"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..1113
                     /locus_tag="OO566_003882"
     CDS             <1..1113
                     /locus_tag="OO566_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR6606968.1"
                     /translation="VWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAM
                     AVAIQHGSFIEDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTD
                     QALNAEIQKGEEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQR
                     DPFDWAEKNLQEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARV
                     FIDNYAAIPSESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFI
                     DARGQRPLKVKDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMH
                     DQPFVQGLTACAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(1125..1937)
                     /locus_tag="OO566_003883"
     CDS             complement(1125..1937)
                     /locus_tag="OO566_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606969.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(1941..2726)
                     /locus_tag="OO566_003884"
                     /pseudo
     CDS             complement(1941..2726)
                     /locus_tag="OO566_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            complement(2723..3391)
                     /locus_tag="OO566_003885"
     CDS             complement(2723..3391)
                     /locus_tag="OO566_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6606970.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(3455..4093)
                     /locus_tag="OO566_003886"
                     /pseudo
     CDS             complement(3455..4093)
                     /locus_tag="OO566_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
     gene            complement(4106..>5846)
                     /locus_tag="OO566_003887"
     CDS             complement(4106..>5846)
                     /locus_tag="OO566_003887"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR6606971.1"
                     /translation="WLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSRETCVAAIGQ
                     AGENLIPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKRLNDYM
                     MTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPPGNQNT
                     VGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGNTCVAN
                     FVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYSKGVFK
                     RVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLAYGSYAIAERWNLGKEY
                     WGYAKNKLWSPFGYPVHHANEASAQVGFIVNCMFNRDCMTHTHINFIGSGLPLKLQRE
                     VAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTVSPLKS
                     RNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKPMQTKDMRNEHDLI
                     CSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQRLGLED
                     IAADLAAHNLLPA"
BASE COUNT         1482 a         1569 c         1349 g         1446 t
ORIGIN      
        1 gtctggcctg atgaagaaga agcaaccaga acgtattttc ccagcccgtg gtcaggcctg
       61 atggaagcaa aggtcgatgc gtgtaacgtg ggtattatgg gaacatcctt gagcggactg
      121 gatgcggcaa tggcagtggc tattcagcat ggttcgttca ttgaagatga taaacaacac
      181 gtcgtttttc accgcgataa cgcaagtgaa aagctaaata ttacgttgat gtcgcgcacg
      241 ggtattttac ccgaagccga tttctattgc cctattccct gcgagccctt acacatcgtc
      301 accgatcagg cattaaatgc tgagattcaa aaaggtgaag agggcctttt ggatcgggta
      361 tttagattga tagtagagga aatcaagttt gctgatccag actggagcca acgcatagcc
      421 ttagagagcc tgaatgtcga ttcctttgct caagcctggt ttgccgagcg caaacaacgc
      481 gacccatttg actgggcaga aaaaaatctc caggaagtcg aacgcaataa acgagaaaaa
      541 catactgttc cctggcgtta tgtcattctg cgcctgcatg aagccgtaca ggaaattgtt
      601 cctcatctga atgaacacga ccataaacgg ttcagtaaag gccttgccag ggttttcatc
      661 gataattatg cggcaatccc ttcagagtct attcgtcgac tactggcctt acgtgaagcg
      721 gggatcattc atattctcgc cctcggtgaa gactacgaaa tggaaattaa tgagtcgcgc
      781 accgtcctga aaacggaaga caacagctac tcgtttgacg tttttattga tgcccgcgga
      841 cagcgtccgc ttaaagtgaa agatattcct ttccctgggc tacgcgagca attacagaaa
      901 acaggggatg aaatccctga tgttggcgaa gattatacgt tacagcaacc cgaagatatt
      961 cgtggacgcg tagcgttcgg cgcgttgccc tggctgatgc acgaccagcc tttcgttcag
     1021 ggacttacgg catgtgcaga aattggtgag gcgatggctc gggcggtcgt aaagcctgca
     1081 tcccgtgctc gtcggcgtct ttcgtttgat taaaggtaaa gctatcagca ctcaacaaac
     1141 tgatgctgtt cattaagacg atagcggact ccggttcgaa tattgttttc accttcaatg
     1201 gcgacggcga agcgaactct ttcgccgtca tgatatgcca gtgcggcgct tccccctagc
     1261 ccgagaataa ttgagtcgac cacgcccgtg ctcactaccg ttgaattttc accgctggca
     1321 atgatgtgta cattgtcgcc gctgttggca attctggcgt ttgcgccaaa gctggcgatt
     1381 tgcaccaggt caccattact ggcgacatga caccgttcac ccaatgtaca aacgcgtact
     1441 cgcattccgg tattcgcaat ccgactacta tctccagcgc tgcttatccg cgaagaatta
     1501 ccggttacag caacccgtgc gcgttcaccg gagctgccaa tgtgactgtt aaagccgaca
     1561 cttccaatcc gcacgctata acccatactg gcaatttgcg cggcataccc agcggttgcg
     1621 attcgggcgt tatcctcaca acagccgatg cgagcatgat cgtcgctgcg ggcaaactga
     1681 ctgcaaaatg gtatctctcc ggggtctgtg gccagttttt gcatagagga aacttcctga
     1741 tgcgcgaagt tttcatccgc cagccatttt ttccacgcat actcaaccag actctccgcc
     1801 cagtccgtat atccttcctc aatcagtgca tgacgaatat cggcataact accgccgcgt
     1861 gggaaatggc gcaggaacca gcgatacatc accgctccga tacgccattc tcgcaggtct
     1921 gctcgtgtaa tgatcattcg ttagtgccgg tgatagccat cgaccatgct tttaaaggtt
     1981 tcgtgtggcg tacgccccgt ggtgcaaaga taaagatgac cgaagataaa aaagaggctt
     2041 ataaatgcca gagcaaaatg cgcctgcaat aaccagtatc ttacgccagg aaacacatct
     2101 cccacggctt gcggatagag acacaacagc cccgtcaata gtaacaacgg cagcaatcca
     2161 tacatgacac caacataggc gacctgctgt aagggattaa atttagactg tgttgttgcc
     2221 gggaaaggat gttcttcccc ctgcataatg ccaaacaaat aaaatcgcgt ttgttttgcc
     2281 gctcgttcca gccacccctg acgacgaatg cgatagtggt gaccattatc cccaacggca
     2341 ttgatcagca caaagccgag ccagcatgcc agtaacaaaa atcagcaaac ttcatgcacc
     2401 gcaaccagac ttttaaccgc agttgcgccc accatcgcaa agtgatttat cagcccactt
     2461 gccagcaaca atacaaagag taacgcattc gaccagtgcc ataatctgac cgctttgctg
     2521 taaagataaa ccttctcacc atgatcggtg gctgatttct tcacgccacg agcacgaagc
     2581 aaggcgtgca gccccagcac caaccacatc ccaaccagca atactccggc aatgatcaac
     2641 cacaccggcc aaaattcggg agtgaactgt ggcacatagt tcgctaactg gctctgaaac
     2701 tgttcagcat gttgcgacgg gttcatacat tttccttttt aatcaaatgt tgaccgaacc
     2761 ggcgatacag atgcggtttt ccggcaccag gtagctgata ttgatagtat ttattatcct
     2821 gcaaccacgc ctgaatttct gggctatctt cgcgaccaaa gatcaatgcg tgttccggac
     2881 aggcgctgac gcaaatgggc ggaaagcctt ttgccagtcg ggactcagcg cagaaatcac
     2941 atttatctgc cactttagtg acagggttaa ggtagcgaac ctggtacgga caagcgccaa
     3001 tgcaataact gcagccgatg cactgcgatt tttctacccg cacgatcccc tgctcatcac
     3061 gccacgatgc ccccgtcggg catacgtcga tgcacggagc atcttcgcag tgctgacatg
     3121 actggcgaaa aaagtgatac tgcgtttcgt tgtcgttatc ggtgactgga atgtgtgcta
     3181 tcgataaacg gcttccctga gcggggacgt ggttagtttt acggcatgcg cgggcgcaaa
     3241 tattacagcc attgcaccgt gactcgtcat gaatcatggc ataccgaact tccttttctt
     3301 gcctcgtgtt cgctaacaga gaagacgcag aaccagtaaa aaagattact gttcccatcc
     3361 ccagaacaaa tttgcgtcga gtgaaggaca tctgattatc ccctaatgca gtggctgcaa
     3421 ctgtccaccc tatccatgac atctccctga aatttcaggg gttctgaaac tgctctacct
     3481 cgaaccattc gcataagcca taagccttgc cataggtcat aaatcgctcg ctaaacaagt
     3541 acaacaccgc cgtcgatgac tccacctgcc gtatatcggc atattcaggt gcaacatcca
     3601 tcgcggcggt cgcggcttca atctgcgctt cctagaagta atatggcgct tgcatcagca
     3661 tggcgacttt gtaaggacga ggatacgtct ggcactcaaa acgaacggcg tgtgcaatgg
     3721 cgcgacaaat gtcctgctcc acaacttgca aagacatcgc cgcgtagttt tcactcatgg
     3781 cctgcgttga gtaatagtaa tcatcctgac ttcccgtcat acaagcgata tccgcgtagt
     3841 ctccgttctg cttaatgctt tccagcaaaa ctgacagttc ctcttctgca accgaatagg
     3901 gtggcgagag aaacacggtg cgcgccacca gctgcccagc cgcagaatgc tggcgaatga
     3961 actcagccag gatttcagca ggttctggtt cggttaccgt atcaacgggg tgacgcatcc
     4021 cctgcaacca ttgccgcttc gcctcgtcaa cttcgctgac tttcgctaac ggcaattcat
     4081 cctgatggtt catcttaccc attccttacg caggcagtag attgtgtgct gccagatcgg
     4141 cggcaatatc ttccagcccc agacgctgca atgtttcgcg ggttggacaa ccaagctgtg
     4201 ggtcccagcc catttctttg tagaacatcg tgagcgaagc atgcatatcg tcacgatcca
     4261 ttttgtcagt accttcagta aagaccggga tctgcggatc cttgtcgaaa acccaggaac
     4321 agataagatc gtgttcatta cgcatatctt tggtttgcat cggttttacc gtgtaggcac
     4381 gatgcaacgt aaagatacgc tctgcagcta aatctaattt ttcctgagtc atctcttcgc
     4441 cggtgatcgc tttgaagaat ttggcttcaa gcgccagatc gccccggtaa ttacggcttt
     4501 tcagcggcga aacggtcatt ggccagaccc agttgcacag ggtgacggcg ttatgcaaac
     4561 agacccgcaa cagcgaccac tttgcatatt taatttttgc gtcattgatt ggcgtgtagt
     4621 ttttggtttc atcgtaagca tcttcagaac caaaaagttc tttcgccact tcacgttgca
     4681 gtttcaatgg caagccggaa ccaataaagt tgatatgggt atgcgtcatg cagtcacggt
     4741 tgaacataca gttaacaatg aaaccgacct gcgctgacgc ttcattggca tggtgaaccg
     4801 gatagccaaa cggcgaccag agtttatttt tcgcgtagcc ccagtactct ttacccaaat
     4861 tccagcgttc tgcgatggca tatgaaccat aagccaggtg actcagctca cccacacgat
     4921 gcgccagacg gtagtaaaaa tctttaatga agttaacgtc acccgcttcc agttgatccc
     4981 agcgaatttc tgcatactct tcagctggca gaacacgctt gaacacacct ttgctgtaac
     5041 aataagtaaa gtcgcgatgc aactgcccgt agttacacca taggccgtag tcatcgaaca
     5101 aattcagacc aaccaggtta ccaatcacac gaccatcgtc tttatcttca aaatctttcg
     5161 gcccgttcgg gaagatggtg gtatggacaa agtttgctac acaagtgtta ccacctgtgc
     5221 tgggcacgcc aaactctttc acccgaggaa tattcatttg ggtcatacaa cggatcgggc
     5281 aagagtggca gccgctcatt tttactgtgt atttctctgc cgccggtcct aagtcaaaaa
     5341 cggatttata ggtacgaaag ccgaccgtat tttgattgcc tggcggaatt tcacccgttt
     5401 caatcggacc accttcagcc gcgccccaaa acagcccttt acgtgctgtc cagcgtgact
     5461 tgggatctga atactctgcc cacgattgtg gcgtacttgg cacgacatgg ttgttattcg
     5521 caccaataag ttcagtcatc atgtaatcat tgagacgctt catctcctga cgatcggcaa
     5581 tgttgacccc tttcgtccct tcaaccgcaa tcgctttcag gtttttcgaa cccattattg
     5641 cgccagttcc cgcaccgccg ctgtggttac ggctattcaa catgccagag agaggaataa
     5701 ggttttcccc agcctgacca atagccgcca cacaggtttc ccgactggtc aatcgacaaa
     5761 tttcttccgt cgttgcgcgc gtcccttttc cccataagaa atcggctttt tccaggctaa
     5821 ctttgtcatc tttaatcttc agccat
//
LOCUS       DAJTXY010000182         5740 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTXY010000182 DAJTXY010000000
VERSION     DAJTXY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.184"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..789)
                     /locus_tag="OO566_003888"
     CDS             complement(<1..789)
                     /locus_tag="OO566_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR6606972.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1035..1661
                     /gene="gstB"
                     /locus_tag="OO566_003889"
                     /pseudo
     CDS             1035..1661
                     /gene="gstB"
                     /locus_tag="OO566_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(1658..2772)
                     /gene="yliI"
                     /locus_tag="OO566_003890"
                     /pseudo
     CDS             complement(1658..2772)
                     /gene="yliI"
                     /locus_tag="OO566_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2883..3266)
                     /gene="bssR"
                     /locus_tag="OO566_003891"
     CDS             complement(2883..3266)
                     /gene="bssR"
                     /locus_tag="OO566_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6606973.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3479..4804
                     /gene="rimO"
                     /locus_tag="OO566_003892"
     CDS             3479..4804
                     /gene="rimO"
                     /locus_tag="OO566_003892"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6606974.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4851..>5740)
                     /locus_tag="OO566_003893"
     CDS             complement(4851..>5740)
                     /locus_tag="OO566_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6606975.1"
                     /translation="THNRSLIFLLGLERFSLLSKSLAMDSENLMFSLFKNGKPVTGDE
                     YNAKNAIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGASCLY
                     LVRRVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRK
                     SFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKV
                     YRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNKCTTVERLKML
                     ADERLYKSKKNGRAQISWQ"
BASE COUNT         1463 a         1482 c         1360 g         1435 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ttcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaat acgttatatc gccgtatgat
      841 aagttcggtg attaataacg cacacacaaa cttaaagaaa ccgtaagttg gtaaagtttt
      901 taaagtttat gcaatatcct gccgcttcgc tgcttctggt gtcatttttt tgccagctgt
      961 tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt cagtctgtgg ctgcctgtcg
     1021 gaaaaggggt gagtatgatt acgctgtggg gtcggaataa ttcaactaac gtaaaaaaag
     1081 tattgctgac gctcgaataa ctggaactac cttatgagca aattctcgcg ggccgtgagt
     1141 ttgggataaa tcacgatgct gattttctgg cgatgaaccc taacgggctg gtgccgttgt
     1201 tgcgtgacga cgaaagtgat ctcattcttt gggaatcaaa cgccattgtc cgctatctgg
     1261 cggcacagta cgggcaaaaa cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg
     1321 aaaaatggat ggactgggca aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg
     1381 gattagtcag aacaccaccg gaagagcgcg atcaggccgc cattgatgcc agttgcaaag
     1441 agtgcgacgc cctgtttgcc ctgctcgatg cggaactggc aaaagtaaaa tggttctctg
     1501 gcgacgagtt tggtgtgggc gatatcgcta tcgcaccgtt catctacaat ttgtttaacg
     1561 ttggcctgac ctggacaccg cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac
     1621 gccccgcagt gcgcaaagtg gtgatgattc ccgttagcta attgcgtggg ctaactttaa
     1681 gtaattcccc actggactcg tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa
     1741 catcacgaat tcgctgccct ctgtccgtta aaatacggcc atcttctgtc actttgtcgc
     1801 cgttgacgct catcacaatg acatctttat ctttcagcgc ccaataaata atttttgctg
     1861 ccactggggg aatttatcgc tgttatagaa ggccatgccg ctcacagcgg gcgaatcttt
     1921 ccagtaaaaa acaggttgct cggtcccggc gacgatctcc cctttcgctt ccggtatctt
     1981 aaagcctgaa tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg
     2041 cgggatatta atttcatcac caccgcgcgg gccatgttca ttcagccaca gtgcattact
     2101 ccacggattc atcgccattc cttgcggatt acgaatgcca taagaccaga tctcgccgcg
     2161 cgcaccggat tcctttataa aaggattatc atccgggatt tcgccctggt cggtcagacg
     2221 caccagtttg ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc
     2281 cagagcaata aaaagataac ctttaccgtc gaataccagc cgcccgccaa aatggttgcc
     2341 ggtagacagt tttggcatct ggcggaagac ggtgcggaag tcggtcactt ttgagagatc
     2401 atcacttaag cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga
     2461 ataacttaac cagatgcggc gagactgagc aaaatcaggc gctaaaacca cgtccagcag
     2521 gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa agcggcgcag ataatccttt
     2581 tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattatc
     2641 gggtaaaaag gccagtgccc agggatggtc gagtttgtct tgcagtactt cgacatttac
     2701 cgttgcagga gccgcccaga gagcggaaga aagacaaata aggggcacaa ggaaaaagga
     2761 ttgtcgatgc ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat
     2821 aacaaattca tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt
     2881 tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc
     2941 atcagacaat tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc
     3001 ccactcatca aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc
     3061 cactcttcct gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc
     3121 agtttaaaaa agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc
     3181 ttcctcagtt gcacgtatgc agcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga
     3241 tcgattcgct gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttgttgcaat
     3301 taaatagtat ggctattttt tgagcaaaat gctgtctagg atcaattctt cacgccgaat
     3361 taacaaaatt tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat
     3421 ccagaactct gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat
     3481 gagcaaagta actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct
     3541 tgtcgattca gagcgtattc tcaccgaact ccgcactgaa ggttatgacg tggtaccgag
     3601 ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca
     3661 agaatcactg gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg
     3721 ttgtctgggg gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac
     3781 cgggcctcat agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa
     3841 acacaaccca ttcctgagcc tggtgccaga acaaggtgtg aaactgacgc cgcgtcatta
     3901 tgcctatctg aaaatttcgg aaggctgtaa tcaccgctgc accttctgca ttattccgtc
     3961 tatgcgcggc gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct
     4021 ggtagatgcg ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt
     4081 tgatgttaaa catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag
     4141 cctgtgcgaa cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta
     4201 tccgcatgtg gacgacgtca tcccactgat ggcagaaggc aaaatcctgc cgtatctgga
     4261 cattccgttg cagcacgcca gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt
     4321 agatcgccaa ctggcgcgca tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg
     4381 ctcaaccttt attgtcggct tccctggcga gacggaagaa gatttccaga tgctactcga
     4441 cttcctgaaa gaagcacgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg
     4501 tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa
     4561 ccgtttcatg cagctgcagc agcagatttc cgccgagcgc ctgcaagaga aagtgggccg
     4621 tgaaattctg gtgattatcg acgaagtgga cgaagaaggc gcgattggtc gcagcatggc
     4681 agatgcaccg gaaatcgacg gcgcggttta tctcaatggt gaaaccaaca ttaagccggg
     4741 tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt
     4801 ttaattaatt caggccgggc aaaatcattt gtccggcctg cgaataatga ttactgccag
     4861 ctaatctgtg ccctgccgtt tttcttactc ttatacagcc gctcatccgc cagcattttc
     4921 aagcgttcga cggtagtaca tttatttgaa tgcgctacac cgatactggt gcttaaatta
     4981 atgcttccca cctggaaatg aacgatttct tttagtatcg atagcaactc ttccagcgtg
     5041 ccgccagtat aaatcaccgc aaattcgtca cctccgaagc ggtagacttt acctttatct
     5101 accgggatct gtgatttaag gattttcacc actttcatca acacttcatc gcccagcaaa
     5161 tgaccgaagg tgtcgttaat gtttttgaat ttatcgacgt caaacagaca caaataaccg
     5221 cctttttccg gcagcgcttt taaatcttca tcaaaggatt ttctgcccat tgctttggtc
     5281 aggtcgtcca ggaacgccga gttatacata ctgtagagtt ttttgacatc ggcagccgaa
     5341 aagaaaagcc cgccatccag tacgcgttca aaatggttat taatgatggc ttctttctcc
     5401 acaataccac gattaatcac tctgcgtacc agatagaggc aactggcacc cgatatcact
     5461 gccaccaatg cggcaaagaa aataatcaat gtacaaaccc gcaaataaac atctttttta
     5521 tacgcaaata cgtacaatcc ggtcggcaaa taggcgaagt gctccatcgc ttccgaaacg
     5581 gtgaagatgg cgtttttagc attatattca tctccggtca ccggtttacc gttcttaaat
     5641 agagagaaca tcaggttctc gctgtccatc gccagcgatt ttgacagtaa agaaaaacgc
     5701 tccagaccaa gcaaaaatat cagactacga ttgtgagtta
//
LOCUS       DAJTXY010000183         5738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTXY010000183 DAJTXY010000000
VERSION     DAJTXY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.185"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            56..574
                     /gene="mddA"
                     /locus_tag="OO566_003894"
     CDS             56..574
                     /gene="mddA"
                     /locus_tag="OO566_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6606976.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            571..1020
                     /locus_tag="OO566_003895"
     CDS             571..1020
                     /locus_tag="OO566_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6606977.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1021..1253)
                     /locus_tag="OO566_003896"
                     /pseudo
     CDS             complement(1021..1253)
                     /locus_tag="OO566_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1339..1512)
                     /gene="ortT"
                     /locus_tag="OO566_003897"
     CDS             complement(1339..1512)
                     /gene="ortT"
                     /locus_tag="OO566_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6606978.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1707..1802
                     /gene="yncL"
                     /locus_tag="OO566_003898"
     CDS             1707..1802
                     /gene="yncL"
                     /locus_tag="OO566_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6606979.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2204..3013)
                     /locus_tag="OO566_003899"
     CDS             complement(2204..3013)
                     /locus_tag="OO566_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR6606980.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3223..4648)
                     /gene="patD"
                     /locus_tag="OO566_003900"
                     /pseudo
     CDS             complement(3223..4648)
                     /gene="patD"
                     /locus_tag="OO566_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4670..5376)
                     /locus_tag="OO566_003901"
                     /pseudo
     CDS             complement(4670..5376)
                     /locus_tag="OO566_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5366..>5738)
                     /locus_tag="OO566_003902"
     CDS             complement(5366..>5738)
                     /locus_tag="OO566_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6606981.1"
                     /translation="RSRQAAYSMILPVQAALERLPPSLLQASADLGARPRQTFRYVVL
                     PLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLV
                     PIILIALYLAFVKRLGAFDAL"
BASE COUNT         1503 a         1450 c         1278 g         1507 t
ORIGIN      
        1 ggctgttttc gatagtattg ataacatcat catcattatt ttattgaggc cagacatgtc
       61 catccgtttt gcccgcaaag ccgactgtgc tgccattgcg gaaatttata accacgccgt
      121 gttgtatacg gcggctatct ggaatgacca aacggtggat gcagataacc gcattgcctg
      181 gtttgaagca cggaccatag caggttatcc ggtgctggta agtgaagaag atggcgtagt
      241 tacgggatat gcctcgtttg gcgactggcg tagtttcgat ggttttcgcc ataccgtgga
      301 acattcggtt tatgtccatc ccgatcatca gggcaaaggt ctggggcgta aattgttaag
      361 ccgattgatt gatgaagcgc gggattgcgg gaagcatgtc atggtcgccg ggatcgaatc
      421 gcaaaatcag gcttcgctgc atctgcacca gtcgctggga tttgtcgtca ccgcacaaat
      481 gccgcaggta ggcactaaat ttggtcgttg gctggatctg acatttatgc agttgcaact
      541 cgacgagcgc actgaaccgg acgcgattgg atgaatcagt cgctcaccct tgcttttttg
      601 attgccgccg gaattggtct ggtagtccag aacacgctga tggtgcgcat cacccagacc
      661 tcctctacca ttctcatcgc catgttgctg aactcactgg tggggattgt gctctttgtt
      721 tctattttat ggtttaaaca aggcatggcg gggtttggcg aactggtgtc cagcgtgcgc
      781 tggtggacac ttatacctgg cttgctgggt tcgttttttg tcttcgccag tatcagcgga
      841 tatcagaatg tgggagcggc gacaactatt gcggtactgg tcgccagcca gctgattggc
      901 ggtctggtgc tggatatctt ccgcagtcat ggcgtaccac tacgggcgtt gttcggccca
      961 atatgtggcg caattttact tgtggttggc gcctggttgg tggccagacg ctcattttaa
     1021 tcagaggatg gtgccgcctt tggttaactg ttcgtggccg cttccatatc ttctttatgc
     1081 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     1141 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     1201 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     1261 ccttagccac ttgtgaaatt tatcgattaa tccaaatgat ataagtctgc gtggtgccgt
     1321 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     1381 ccatgtggcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     1441 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     1501 atagagagac atgtacgggc gtccagccat atgaataaaa cgcgcgcaaa tatgcgccat
     1561 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     1621 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     1681 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     1741 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttattt
     1801 gatgtagtga ttttgcacgc agcacgtact gtcagagaaa tcaactctct gacagtacgc
     1861 aagatcttct catactctcc aagtttgcaa cattatcctt aatatttact tctttttgct
     1921 ggcatctaca ttgaaatgag tagaaaacat ccccctactg tcgtctatac aaacagctta
     1981 ctgattcaaa cggaatcttg ttttcacatt tttctatcac gaatacttta tacgcgcggg
     2041 agtctataaa tgctttatac tttcaaggaa taacgtaaat ataaaaagat ggcgtaaagt
     2101 atatgcatgg caggatgttt gcaacctcct tgatgacgct gccccattga aattgcaaac
     2161 cccgccctgg ttatcttgcc catttatcat gcaaaacaga taattatagg cctctgaata
     2221 cctcagaaag attgaatttt ttatagtccc tctcttgaat atttccattt gatatcaatc
     2281 gattcatctc ttcaacaaaa taatttatat catcacactt gttattatcc ggaagatctg
     2341 agtagttata attccaccat tttattctca acagttcatc aataacattt gaatcaaacc
     2401 taaatctgat tatttttgca ggaactcctg caactatggc atatgggggc acatctttgg
     2461 taacaacgga attggcagca ataacagcac catcacctat tgcgatgcca cctttcaata
     2521 caacatcatt tcctatccag acatcatttc ctataatcgg attttcaggt ttggtaatga
     2581 atggtttcaa tgtccagcta cgttcaaatt cactcatagc aaacttatca aattcaccgt
     2641 tataagtcat catgtgagta gtaaaacgat tcaaaggatg atttccaccc attattttta
     2701 catttgatgc aatactacaa aatcgaccta tacgtatatt ccctgaaaaa tggcaggtcg
     2761 gaaatgaaaa agcgccgaca ctggagaaat ttttcacagg cattgttgaa tattcttcag
     2821 caataacatt atcttcaatt gttatcttgt caccttttga gaaaagaaat aaattccttt
     2881 ctttatacga cttcttcccc ttatgattta aaaagatttt attatccata caaaactcaa
     2941 agtgtttttt cgtccatctg aaggtaaggg ttttattttt atcttccgtg aatttgtaca
     3001 attgaacttt cattgttaat acttctactt ataaattatt agtttaataa cgatactcta
     3061 aagccttatg ctaatcaact gtgacaggat gaaaaatcgc catcaaacat ttagcctaat
     3121 ttttacgcca gttattaatg taatcattct aatacttaac cgccattaaa ctcagaacct
     3181 aaaacgatag tggccgtcct ataacagcca ctaacatcta acttaatgtt taaccatgac
     3241 gtggcggacg acggtgtaat cctccagccc ataaagtgac atatccttgc cgtaaccaga
     3301 aagtttctgc ccaccgtgcg gcatttcact taccagcatg aaatgggtat tgacccaggt
     3361 acaaccatat tgcagccgtg cgctgacgcg atgcgccctg cccacatctt tcgtccatac
     3421 cgaagatgca agtccgtact ggctgtcatt cgcccagttc accacctgtt cttcgttgtc
     3481 gaagggcgta acactcacta ctggaccaaa tacctctttt tgcaggatgg catcgtcctg
     3541 taatgcgcca gccagcagcg tcggcgcata gtaatagcca ttacccttgc gcttttcacc
     3601 gccagtgatc actttgatgt gccctgtcgc tttcgcctct tctactgcct tgctgacgcg
     3661 ttcgagatgc gccagcgagc ttaaaggtcc aagctccgta gactcgtcat ccggcgcacc
     3721 agattttaac gttgccaccg caggcaccca gtttttccac cagcgtatcg taaatgcctt
     3781 tttgcgcgta gatccgacaa gccgcagtac aatcctgtcc agcattgtaa tagccaaatg
     3841 tacgtacacc ttcgaccact gcttcaatat ccgcatcatc aaaaacaatc actggcgctt
     3901 tgccaccaag ttccatatga gtacgcttaa tggacgacgc ggtatggctg atgatgtgct
     3961 cgccggtggc gatagagccc gtcagcgaca ccatccgcac tttgggatga ccggtcagcg
     4021 gatcacccac cgttttgcct ctgccaaaca gtatgttaat cacgcctgcc gggaagatat
     4081 ctttcgccag ctctgccaac ttcaacgcgg tcagcggggt aatttctgat ggtttaagca
     4141 ctacgcagtt ccctgccgcc agcgccggag caagtttcca cgcggccatc atcagcggat
     4201 aattccacgg tgcgatagaa gccacgcccc ccaacggatc gcgacggatc atcgaagtat
     4261 gaccttcaaa atattcacct gccgccagac cattcagaca gcgcgccgca cccgcgaaaa
     4321 agcgaaaaac atcgacaatc gccgggattt catcattgaa cgcactatgc agcggtttgc
     4381 cacaattacg ggattccagt tcggcaaaaa cctgaccatt ttcttcgata acatccgcca
     4441 gtttcagcag gcattccgca cgcgctttcg gtgtggtttg cccccattca gcaaatgctg
     4501 catctgccgc acacacagca gcatcgacct gctctgcgga tgcctcggca atctccagta
     4561 aaacgtcccc cgttgccgga ttatagacag gctgtttttc cccttcgccg ctaaccagtt
     4621 ctccgttaat cagtaactta tgttgcatag attattcctg tatcagtggt tattgaccat
     4681 tgtcgccttc gcgggttagc caccaggccc ccaggatcgg caaggttgtt accaacataa
     4741 ccagcagtgc caccacgttg gttaccggta catcacgcgg tcgcccaagc tgattgagca
     4801 accacaacgg taacgttcgt tcatgacctg ccgtaaaggt cgtaacgatg atttcatcga
     4861 acgacaaggc aaacgccagc attcctcctg ccagtaacgc cgaactgaga ttcggcaaca
     4921 ctacgtagcg gaaggtttgc cagccattgg ccccaagatc cattgacgcc tcaaccagac
     4981 tccacgaggt gcggcgaaaa cgggcgatga cattgttaaa caccaccact acgcaaaaag
     5041 tcgcatgacc gaccacgatg gtgaaaaatc ccggctccag attgatggtt ttaaaggcgg
     5101 ttaatagcgc cagaccagtg acaatgcccg gcagcgcaat gggcagcagc aataacagcg
     5161 aaatggcgtt tttgccgaaa aagtctcgtc tccacagcgc ggcagctgcc agcgtcccta
     5221 acaccagcgc agcgtcaggc cctgcggtgg aaaactaaac gccgcatctt cagtgttaaa
     5281 cgcataggcg gcgattatca ggatgggaaa atgtaggaaa acaacgccgc cccaggccgc
     5341 cagtttgagg aaaaacggtg cgcgttcaga gtgcatcgaa cgctcccaga cgtttcacga
     5401 acgccaggta cagtgcgatg agaataatcg gcaccagggt gaatgccgcc gccatcggca
     5461 tattgccaat cgccccctgc tgggaataaa ccatattgcc gataaaatat cctggaggac
     5521 caaccagctg cgggacgata aaatcgccca gtgtgagtga gaaggtaaag atagagccag
     5581 cggcaatacc cgggattgcc agcggcagca ccacatagcg aaaggtttgt cgtggacgtg
     5641 cgccgagatc agccgacgcc tgcaacaatg acggcggcaa acgctcaagc gccgcctgaa
     5701 cgggcaggat catcgagtag gcagcctgac ggctgcgg
//
LOCUS       DAJTXY010000184         5698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTXY010000184 DAJTXY010000000
VERSION     DAJTXY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.186"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            47..1515
                     /locus_tag="OO566_003903"
                     /pseudo
     CDS             47..1515
                     /locus_tag="OO566_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(1551..1754)
                     /gene="ydfZ"
                     /locus_tag="OO566_003904"
     CDS             complement(1551..1754)
                     /gene="ydfZ"
                     /locus_tag="OO566_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6606982.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1932..2618)
                     /gene="ydfH"
                     /locus_tag="OO566_003905"
     CDS             complement(1932..2618)
                     /gene="ydfH"
                     /locus_tag="OO566_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6606983.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSNWFSEE"
     gene            complement(2707..3453)
                     /gene="ydfG"
                     /locus_tag="OO566_003906"
     CDS             complement(2707..3453)
                     /gene="ydfG"
                     /locus_tag="OO566_003906"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6606984.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3590..5635
                     /gene="dcp"
                     /locus_tag="OO566_003907"
     CDS             3590..5635
                     /gene="dcp"
                     /locus_tag="OO566_003907"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6606985.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1425 a         1356 c         1475 g         1442 t
ORIGIN      
        1 tataacaaaa catatagctt gccatattta aatttaagga attgttatgg gaaataattt
       61 gttatcagca aaagcgacac tccctgttta tgatcgtaat aacctggccc caagaattgt
      121 tcatttaggc tttggtgcat ttcaccgtgc gcatcagggc gtgtatgccg atattcttgc
      181 tacagaacat ttcagtgact ggggatatta tgaggtcaac ttaatcggcg gcgaacagca
      241 aattgccgat ttacaacagc aagataatct ttataccgtt gcggaaatgt cggccgatgc
      301 gtggacggct cgcgtcgttg gcgtcgttaa aaaagccttg cacgtacaga tggatggctt
      361 agaaaccgtg ttggctgcga tgtgtgaacc gcaaatcgcg attgtctctc tgacaatcac
      421 cgaaaaaggg tatttccact ctccggcgac cggacagtta atgctcgatc acccgatggt
      481 cgctgccgac gtgcaaaatc cccaccagcc gaaaacagca acatgggtga ttgttgaggc
      541 gctggctcgc cgtaaagcgg caggacttcc cgcattcccg catttaccgt catgtcatgt
      601 gacaacatgc cagaaaacgg tcatgttatg cgtgacgttg tcacttccta cgcgcaagcc
      661 gttgatgtaa aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg
      721 gaccgtattg tgcccgcagt ggcagaggat acgctggcga aaatcgaaca acttaccggt
      781 gtgcgcgatc ctgctggcgt tgcctgtgaa cctttccgcc agtgggttat cgaagataac
      841 tttgtagccg gacgtccgga atgggaaaaa gcgggagccg aactggttag tgatgtgctg
      901 ccttatgaag agatgaagtt gcgcatgctc aacggcagtc attcattcct ggcatatctg
      961 ggttatcttg ctggatatca gcacattaat gactgtatgg aagatgaaca ttatcgtcat
     1021 gcggcgtatg gcatgatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat
     1081 ttgcaagatt acgctaaccg attaattgca cgctatagca acccggcgtt acgtcatcga
     1141 acctggcaga ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt
     1201 cgctggcatc tggcgcatga cagcaagttc gatctgctgg cgctgggcgt cgcgggttgg
     1261 atgcgttatg tcggtggtgt tgatgaacag ggaaatccga tagaaatcag tgacccactg
     1321 ttacctgtta ttcagaaggc tgtacaaagt agtgccgaag ggaaagcgcg cgtccagtca
     1381 ttgctggcga ttaaggcaat ctttggtgat gatttgccag acaatagctt gtttactgca
     1441 aaagtgacgg aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa
     1501 tattccgtga agtaaaaaaa gcaatgccgc catcggcggc attcacacac ttagttcatg
     1561 ccgagacgaa tcaggtccag aggtgccagt ttctcttcac aaccttcaac aactacggtt
     1621 tttccgcggc ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca
     1681 gtgtgaccgg tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga
     1741 tcgtaggtcg tcataggtgt tttctccttt ctgatttaca gtcgaagccc acgtaacggg
     1801 cgcaatataa atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt
     1861 ttttgatgca tgcatgagcc gtaaggcggg gggaaaagcc tctcatcccg gatgggatga
     1921 gagtaaatga attactcttc gctaaaccag ttgctgtttt cctggcggat ctggcgcacg
     1981 gattcgctga tttcctgcaa atgttgcgtc attgcacgtt ctaccgcatc gccatcacgt
     2041 ttttgcaggg cagagaaaat atcaagatgc tggcgtaaca gcatttctgg tggagaaaca
     2101 tggtcgaaac tcatatagcg cacgcgatca acggtcgctt tcaggttctc aatggtatcc
     2161 cacgccagtt gacagtcggc aatctgcgtc aggagttgat ggaagttgtc atcaagttca
     2221 aaaaaatcat ccagttgctt gcgctcaatg gcaatgcgtt gctggtgaag attttgttcc
     2281 agttggtagc actggctttc ggtaatcatg ctcgccgccc gacgcgccac cgcgcactca
     2341 atggcctgac ggataaaact gccgttgcgc acctgggcca tggaaatttt gttgacgtag
     2401 ctgccacgct gcggacgaat ttgaatcagg ccgttttccg ccagtttaat aaaggcttca
     2461 cgaaccggct ggcgtgacac attgaaacga acagaaactt ctttttccga caacggtgtg
     2521 cctggtgcaa tcaggcaatg gacaatgtcg cgacgaagaa tacgataaat ctgctgattg
     2581 acaggctgtg tgggattaag ttgcgtttcg acggtcattc gttcttgctt gtgagtgagt
     2641 taactgcatg agtctaccac ttttttgtga caagcaataa cccggcagtt acgccgggta
     2701 taaaaattac tgacggtgga cattcagtcc ggcatagctt tgggtaaccg gcatcatttc
     2761 cagggtattg atattgacgt gagcaggcag cgttgacacc caccagacgg cttcgctgac
     2821 atcttctggc gtcaatgcaa cggtattttg ataggttttt tccgctttac cgtcatcgcc
     2881 tttaaagcgg acattggaaa actcggtgcc acccaccaga cccggttcga tgtcggtgac
     2941 gcgcaccgcc gtaccatgca gatccgtacg cagattcagg ctaaactgac gaacaaacgc
     3001 tttcgtcgca ccgtaaacgt taccaccggc atacggccag ctgcctgccg ttgagccaat
     3061 gttaataata tgaccatgat tacgttcaac catacccggt aagacggcgc gcgtcatata
     3121 tactaggcct ttgttgttgg tatcaatcat cgtttcccag tcttcaacgc tggctttatg
     3181 cgcaggctcc atgcccaacg ccaggccggc attatttacc aggatatcaa tattgctcca
     3241 ctcggcaggg agcgatgcca gcatctcttc aatagcggcg cggttgcgaa cgtccagttg
     3301 ggcgatatac agattatctc ccagttcgtc ttttaactcc tgcaaccgct cctggcgacg
     3361 gccagtggcg ataactttat gcccttgttg aataaaacga cgagtaatgc attcaccaaa
     3421 acctgccgtt gctccagtta ctaaaacgat catctcactg ttcctcaacg cttttgtgtg
     3481 tcaataacat agcacgtgat gcttaagagc gggtaatact ggtttgcaag attgcactcc
     3541 gttatcagta agcctacgct atggaacacc tcgcgttcag gagaagaaaa tgacaacaat
     3601 gaatcctttc cttgtgcaaa gcacactgcc gtatctggct cctcattttg atcaaattgc
     3661 caatcatcac tatcgcccgg cattcgatga gggaatgcaa caaaagcggg cagaaattgc
     3721 tgccatcgcg cttaacccgc aaacgcctga tttcaacaat actattctgg cactggaaca
     3781 aagcggagaa ttaattaccc gcgttaccag cgtctttttt gcgatgactg cggcgcatac
     3841 caatgatgaa ttacagcgtc ttgatgaaca gttttccgct gaactggcgg aactggctaa
     3901 tgatatctat ctgaacggtg aattattcgc gcgggtagat gctgtctggc agcgccgtga
     3961 atccctgggg cttgatagtg aatccatccg cctggtggag gttattcatc aacgttttgt
     4021 ccttgccgga gccaaacttg cacaagctga taaagcaaaa ttaaaagtac tgaatacaga
     4081 agctgcgacc ctgaccagcc agtttaacca gcggttactt gcagcaaata aatccggcgg
     4141 tctggttgtg aacgatatcg cgcagctggc aggaatgagt gagcaagaga ttgcgctggc
     4201 ggcagaggcg gctcgcgaga aaggtctgga taacaaatgg ctgattccgc tgctgaatac
     4261 cacccaacaa ccggcgcttg ccgaaatgcg cgatcgtgcg acgcgtgaaa aactgtttat
     4321 tgcgggctgg acgcgagcgg aaaaaaatga tgccaatgat acccgcgcta tcattcaacg
     4381 tctggtagag attcgcgcac agcaggcgaa actgcttggt tttcctcatt atgccgcatg
     4441 gaaaatcgcc gatcagatgg caaaaacgcc agaagcagca ctcaacttta tgcgggaaat
     4501 tgttccagcg gcgcgtcaac gtgcgagcga tgaattagcc tccatacagg cggttatcga
     4561 taagcagcag ggcgggttta gcgcgcagcc gtgggactgg gcattttatg cggaacaggt
     4621 acggcgggag aaatttgatc ttgatgaggc gcagctcaag ccatattttg aattaaacac
     4681 ggtgttaaat gaaggtgtat tctggaccgc gaatcagctc ttcggtatta agtttgtcga
     4741 acgttttgat attccggtct accatcctga cgttcgtgtg tgggaaattt ttgatcataa
     4801 tggcgtggga ctggcgttat tttacggtga tttcttcgcc cgtgattcaa aaagcggcgg
     4861 tgcatggatg ggcaattttg ttgagcaatc aacgcttaat gaaacgcatc cggtaattta
     4921 taacgtctgc aattatcaga aacccgctgc cggtgagcct gcgttgttac tctgggatga
     4981 tgtaataacc ttattccatg aatttggtca tacgctgcac ggcctttttg cccgccagcg
     5041 ttatgccacg ctttccggca ccaacacgcc gcgtgatttt gtcgaatttc cgtcgcaaat
     5101 caacgaacac tgggcaacgc atccgcaggt attcgctcgc tacgcccggc attatcagag
     5161 cggggcagca atgcctgacg aactgcaaca gaaaatgcgt aatgccagcc tgttcaacaa
     5221 agggtatgag atgagcgaac tgcttagcgc cgcacttctc gatatgcgct ggcattgcct
     5281 ggaagaaaac gaagcaatgc aggatgtcga tgattttgaa ttgcgggcgc tggtggcgga
     5341 aaatatggat cttcctgcta taccgccacg ctatcgcagc agttatttcg cccatatttt
     5401 tggtggcgga tatgctgcag gttattacgc ttatctgtgg acgcaaatgt tggccgatga
     5461 tggttaccag tggtttgttg agcagggcgg attaacgcgt gaaaatgggc agcgttttcg
     5521 cgaggcgatc ctttccagag gtaacagcga agatctggaa cgcctgcatc gacaatggcg
     5581 cggtaaggct cctcagatta tgccgatgct gcaacatcgt ggcttgaaca tataaacacc
     5641 accaatggct ggcctggaga tatcgccagg ccagcctggt tatagcagta attgcagc
//
LOCUS       DAJTXY010000185         5613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTXY010000185 DAJTXY010000000
VERSION     DAJTXY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.187"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(152..1501)
                     /gene="lysC"
                     /locus_tag="OO566_003908"
     CDS             complement(152..1501)
                     /gene="lysC"
                     /locus_tag="OO566_003908"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6606986.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2026..3675
                     /gene="pgi"
                     /locus_tag="OO566_003909"
     CDS             2026..3675
                     /gene="pgi"
                     /locus_tag="OO566_003909"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6606987.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4137..4379
                     /locus_tag="OO566_003910"
     CDS             4137..4379
                     /locus_tag="OO566_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606988.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            4493..5131
                     /locus_tag="OO566_003911"
     CDS             4493..5131
                     /locus_tag="OO566_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6606989.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5128..>5613
                     /locus_tag="OO566_003912"
     CDS             5128..>5613
                     /locus_tag="OO566_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6606990.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FT"
BASE COUNT         1422 a         1463 c         1435 g         1293 t
ORIGIN      
        1 cgataattaa ttagagatgc agatgaaaaa attaaaggca attattctcc gataaagacg
       61 ttggttaaca gagtacaggc tcgtttattg atgagcatag tgacaagaaa atcaatacgg
      121 cccgaaatat agcttccagg ccatacagta tttactcaaa caaattacta tgcagttttt
      181 gcaccacctg ctcggcatct tcgccgggca ccaggaagca caggttatgg ctggatgcgc
      241 cgtaacaaat catgcgaatg ttgaacggtt ccagtacgcc gaatacctct ttgccaacgc
      301 cgcaggcttt tgacaggtca ttgccaatca acgcgaccag cgccagacct tcttccacct
      361 ccacccggca cagtgcggaa agctccatca gcagagattg cgtcagcaac gtatcgccag
      421 tggaggttga accggtggta tcaagggtta atgccacgct cacttctgac gtggtgatta
      481 agtctaccga aatattatgc cgcgcgagga tgccgaaaac ttccgcgagg aagccgcgag
      541 aatgcagcat attcaggctg tgcaaagtga gcagagtctg attgcgacga agcgccagcg
      601 cgcggaacag cggcggattt tcagttttat tgcacaccag cgtaccacct gcgcgtgggt
      661 ctttgctgga gccgacaaag accgggatat cgctgcgtac tgcgggtagc aacgttgccg
      721 gatgcagtac ttttgcacca aaagttgcca tctctgccgc ttctgcaaac gcgatttcat
      781 caatgcgttt tgctgcggaa actacgcgtg gatcggtggt gtagatgccc gggacgtcgg
      841 tccagatatc aacacgagat gcgtgtaaag cctccgccag caaggctgcc gtataatcgc
      901 tgcctccacg gccaagcgtc gttgtacgac ctttattttc gctaccgata aatccctggg
      961 tgatcactaa gccttcattg aggcgtggga gcagctgcag cgcggccagt tccgccagcg
     1021 cggctacatc tggctctgca cgaccaaatc ggtcgttggt acgcatcact ttacgtacat
     1081 caaaccactg tgcctgaaca tcgcgttcgc gcaggatttc gacaaacagc agggtcgaca
     1141 tcagctcgcc gtggctgacc agctcatctg tcagcgccgg agacgttgcc agcgccgccg
     1201 cttctgccag aacagtaatg ttctccagca gacgttcaat ctcttcacgg ataacgttcg
     1261 ggtaacgcag acgttccaga atggcaaact ggatgttgcg aatagcgtcg agtttttcga
     1321 atcgctcgcc aggttccagc ccttccgcta aagcgaccag cagattagtg ataccagcag
     1381 aagccgagag gactactaaa cgcacgttgg catcagaaag cacaatatca gcgctgcggt
     1441 tcatggcgtc aaaatcagct acgctggtac cgccaaattt ggagacaaca atttcagaca
     1501 taactacctc atgtcagggg atccattttc agccttggca caagggaaga gcggaagacg
     1561 ggtgggcgca gagcgatact tcgctactat tttcacccag aagtgctcca ccacttgcga
     1621 aacgcccgac tgcgaacgct tctggtgaca acccagggga ttcagcccct gtagccgatg
     1681 atgaacgtgg ccagccgttc aatcacctcg gcgatgcacc ccctcaggtg ttatcacagg
     1741 actggctcct ccaacaccgt tacttgggca acgcgcctct tctggcctgc gctagcgcag
     1801 gtagtacatt tataaataaa gggtgagcgg ggcggttgtc aacgacgggg tcatgcggat
     1861 ttttcatcca ctcctggcgg tcagtagttc agctaataaa tgcttcactg cgctaagggt
     1921 ttacactcaa cattacacta acggcactaa aaccatcaca tttttctgtg actggcgcta
     1981 caatcttcca aagtcacaat tctcaaaatc agaagagtat tgctaatgaa aaacatcaat
     2041 ccaacgcaga ccgctgcctg gcaggcacta cagaaacact tcgatgaaat gaaagacgtt
     2101 acgatcgccg atctttttgc taaagacggc gatcgttttt ctaagttctc cgcaaccttc
     2161 gacgatcaga tgctggtgga ttactccaaa aaccgcatca ctgaagagac gctggcgaaa
     2221 ttacaggatc tggcgaaaga gtgcgatctg gcgggcgcga ttaagtcgat gttctctggc
     2281 gagaagatca accgcactga aaaccgcgcc gtgctgcacg tagcgctgcg taatcgtagc
     2341 aataccccga ttctggttga tggcaaagat gttatgccag aagtcaacgc ggtgctggag
     2401 aagatgaaaa ccttctcgga agcgattatc tccggtgagt ggaagggtta taccggcaaa
     2461 gcaatcactg acgtagtgaa catcgggatc ggcggttctg acctcggccc gtacatggtg
     2521 accgaagcac tgcgtccgta caaaaaccac ctgaacatgc actttgtttc taacgtcgat
     2581 gggactcaca tcgcggaagt gctgaaaaaa gtaaacccgg aaaccacgct gttcttggta
     2641 gcatctaaaa ccttcaccac tcaggaaacc atgaccaacg cccatagcgc gcgtgactgg
     2701 ttcctgaaag cggcaggtga tgaaaaacac gttgcgaaac attttgcggc gctctcaacc
     2761 aatgccaaag ccgttggcga gtttggtatt gatactgcca acatgttcga gttctgggac
     2821 tgggtcggcg gccgttactc tttgtggtca gcgattggcc tgtcgattgt tctctccatc
     2881 ggctttgata acttcgttga actgctttct ggcgcacacg caatggacaa gcatttctcc
     2941 accacgcctg ccgagaaaaa cctgcctgtc ctgctggcgc tgattggcat ctggtacaac
     3001 aatttctttg gtgcggaaac tgaagcgatt ctgccgtatg accagtatat gcaccgtttc
     3061 gcggcgtact tccagcaggg caatatggag tccaacggta agtatgttga ccgtaacggt
     3121 aacgttgtgg attaccagac tggcccgatt atctggggtg aaccaggcac taacggtcag
     3181 cacgcgttct accagctgat ccaccaggga accaaaatgg taccgtgcga tttcatcgcc
     3241 ccggctatca cccataaccc gctctctgat caccaccaga aactgctgtc taacttcttc
     3301 gcccagaccg aagcgctggc gtttggtaaa tcccgcgaag tggttgagca ggaatatcgc
     3361 gatcagggta aagatccggc aacgcttgac tacgtggtgc cgttcaaagt gttcgaaggt
     3421 aaccgcccga ccaactccat cctgctgcgc gaaatcactc cgttcagcct gggtgcgttg
     3481 attgcgctgt atgagcacaa aatctttact cagggcgtga tcctgaacat cttcaccttc
     3541 gaccagtggg gcgtggagct gggtaaacaa ctggcaaacc gtattctgcc agagctgaaa
     3601 gatgataaag aaatcagcag ccacgatagc tcgaccaatg gtctgattaa ccgctataaa
     3661 gcgtggcgcg gttaatcatc atgcgtgcct gatgccgata tgtaggccgg ataaggcgtt
     3721 tacgccgcat ccggcaaccg gagcctgatg ccaatatcga cattaatgca tagtatgagg
     3781 agttaaataa ccttaaaaac aatgccgaca ataagtcggc attgttttat cagataaatc
     3841 cccttgtctg tgatttaacg gaaatcatac cgtgaggtta atcctaaaat agatttttaa
     3901 tcgttgttta tttcggagaa tacgcagatt aattactttt gtttttattt taagtttatg
     3961 atttttattg ttatttaaat ataagttgaa acttatattt gatattcatt ccaattatcc
     4021 taaaactcca tcgctaattc cccgcgccgt aattcgcatg ctttagttgt gtatactcga
     4081 tcccgcccga aatgtttttg ggtaaatctc cattcattca atgaagggaa attgttatga
     4141 aaaaagttct gtatggcatt tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg
     4201 cacctgtaca ggtgggcgaa gcggcagagt cggcagcaac gtcggtttcg gcggggagtt
     4261 cctccgcgac cagcgtcagc accgtaagct cggcggtggg tgtcgcgctc gcggtaaccg
     4321 gtggcggtga tggttctaat accgggacca ccacaaccac gaccaccagt acccagtaat
     4381 aaagtatgta tccccaaaat aatttgagtc attgcatctg tggcttgaag tatgaaggga
     4441 tttaccataa ccacactccg gtgtggttat tctgcccctc tggagaagag ttgtgaagcg
     4501 acctgcactc attcttatct gcctgctatt acaggcctgt tcagccacga ctaaagagct
     4561 gggcaattca ctgtgggaca gtctgttcgg cacgccaggc gtacagctga cggacgatga
     4621 tattcaaaat atgccctacg ccagccagta catgcagctt aatggcgggc cgcaattgtt
     4681 tgtggtgctg gccttcgctg aagacggaca acaaaaatgg gtcactcagg atcaagccac
     4741 tcttgtcaca caacatggtc gtctggtgaa gactttgctt ggcggcgaca acctgattga
     4801 agtgaataac cttgccgccg acccgctgat taaacccgca caaattgttg atggtgcaac
     4861 atggacgcgc acgatgggct ggaccgagta ccagcaggta cgctacgcca ccgcacgctc
     4921 agtcttcaaa tgggatggca ccgataccgt caaggtcggc agcgatgaaa ccctggttcg
     4981 cgtgctggac gaagaagtct ccaccgacca ggcgcgctgg cataaccgct actggatcga
     5041 cagcgaaggg caaattcgcc agtcggaaca gtatctcggc gcggattatt tcccggtgaa
     5101 aaccactctc atcaaggcgg caaaacaatg attaaacaaa ctattgtcgc gttgcttttg
     5161 agtgtgggag cgtcatcggt cttcgcggca ggaaccgtca aggtgttcag caatggcagc
     5221 agtgaggcca aaacgctaac gggcgcagag catttaatcg atctggtagg gcaacctcga
     5281 ctggcaaaca gctggtggcc tggtgcggtg attagcgaag agctggcaac ggcggcagca
     5341 ttgcgtcagc agcaggcgtt gctgacacgg ctggcagagc agggcgcaga ttccagcacc
     5401 gacgatgccg ccgccattaa cgccttacgc cagcaaatcc aggcattgaa agtgacaggc
     5461 aggcaaaaaa tcaatcttga tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg
     5521 ttgcagggca actacacgct gtgggtcgga ccaccgccgt ccacggtcac gttgttcggg
     5581 cttatcagcc atcctggcaa tcagccattc act
//
LOCUS       DAJTXY010000186         5594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTXY010000186 DAJTXY010000000
VERSION     DAJTXY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.188"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..404
                     /locus_tag="OO566_003913"
     CDS             <1..404
                     /locus_tag="OO566_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6606991.1"
                     /translation="QQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCY
                     YSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGIDPE
                     AYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            436..1050
                     /gene="istB"
                     /locus_tag="OO566_003914"
                     /pseudo
     CDS             436..1050
                     /gene="istB"
                     /locus_tag="OO566_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1219..5313)
                     /gene="sepA"
                     /locus_tag="OO566_003915"
     CDS             complement(1219..5313)
                     /gene="sepA"
                     /locus_tag="OO566_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR6606992.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWFSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1379 a         1399 c         1329 g         1487 t
ORIGIN      
        1 ggcagcagcg aagcaaagcg ttactgacgt cgctgaatga atggatggtg gagaagaatg
       61 gtacgctgtc gaaaaaatcc agactgggcg aagcgttcag ctatgtactg aatcagtggg
      121 atgccctctg ttattacagt gatgacggtc tggcggaggc ggacaataac acagcagaaa
      181 gagcgcttcg taccgtctgt ctcgggaaaa agaattacat gttcttcggc agcgatcatg
      241 gaggcgatcg cggagctctg ctgtacggac taatcgggag ctgtcggttg aacggtatcg
      301 atccagaagc gtatctgcgc catatcctga gcgtactgcc ggaatggcct tccaaccgag
      361 ttgacgaact gctgccatgg aacgtagttc tcaccgataa ataagcgtca atacggcctt
      421 ccgttgacgc ttacagaact cctgtggcgc gagagtgaaa aactgcgtcg ttataaaaag
      481 gaggcccggt tgccagttgc caaaacgcta agcgaatacg acttcattca gttgccggaa
      541 ctgaatggag ctcagttcca gcaactctgt gaaacgacag actgggttga tgctggagaa
      601 aacgttctgc tgttcgggga cagcgggttg gggaaaagcc atctggcggc agcgatcgtg
      661 gatggcgtag taggccaggg ctaccgggcc cggttcaaca gcgcaggaga gctgttgcag
      721 gaactacata aagccagagc tcagttgaaa ctgaatgagc tgctactgaa actggatcgc
      781 taccgggtga tagtggtgga tgatcttggc tatgtcaaac gcgacagcgc cgaaacgggt
      841 gtgctgttcg agttaatagc gcatcgctat gaacgtggga gcctggtgat aaccagtaac
      901 catccgttca gcatgtgggg cagcatcttc gtggatgaga ctatggcggt ggcggcagca
      961 gaccgactga tccatcacgg atatatgttc gaactgaaag gggaaagcta caggaaaaag
     1021 acagcgaagg cagtaacaag cgtgacttga tgtcgcactg aaggggcggc cagtatagtt
     1081 ggcgccagtc ggcaaaacta gttgacgtct aatacctgga cggtcacttc tgagaaaagg
     1141 catttacaca gaatcttaaa caggctcacg gagttctttc agaaaaaggc ctgccgaatg
     1201 gcaggcctat cccattcgtc agaaaacata acggaagttg gcgtttatcg cattatccac
     1261 attatatttc ccgaacgctg atttctccag ctccagtccc aaacgcatat tatccttaat
     1321 ttccgcattc atccctaccg tcatcagcat cctgctatct ttttcacctt cgaaacgttt
     1381 ctctccggaa gcatcctgca gaaccgtttc tccgttcgcc agcaggtcga actgataacc
     1441 cagcccggct cgagctgtga ttttccagtc gtctccggag aaggctcttc ccacgtcaac
     1501 accagtacgg ccaatcagtg ggttataatc cttgtctttc atgctcagag ccattccccg
     1561 gtcttcccag ctaaaagctt ttcctgatac agaaccgtaa accagctcta tctgtggctc
     1621 cacccaggac tctttcgtca ggtggtaacg ataaccaact tcagcaccgg tataccagga
     1681 atgagagctg tagtcttttg ttcccagtga ggcaaaattc gccgtgtgct gattatcatg
     1741 atggagatat ttaccaatca ggtcaaaata agctccggaa ttaaagagtg cagaggcata
     1801 cagaccgcca cccacggatt tgttttttcc actgaatgcg tggctgcttg cattgctgtc
     1861 cgtataggtc agcaatgccc ccgtaaataa atccacaccg tccagctcat gctttctgtc
     1921 gacaccaatc tgaacgtgag tgtagttatc gctgtagtca ccatctgccg aaccggtacc
     1981 attcattatg cgtgcccaga caccggcatc cccctgagta tcccgcaggt cacccatacg
     2041 tttgttcagg ttattgacct ctgtaaggaa ggtcttataa cctgatgcca taaagtcttt
     2101 tgcggctttc gaggctccgg catcagcggt agtctgatat cctgtcagca cccattttgt
     2161 ggcatcatcc gttttttccg tgctgattac tggcgtaacg ttactgaaac caatctgctg
     2221 cgtaccggca gtgaacgttt tctcatcact ccctttcggt gcggtaatta acgtcacgct
     2281 catttcactg gctggtttgt ttaaaaagtc aaccaggacg ctattattac tgcctgacag
     2341 tttgtctgtg acattcaact ggtcagcccg gctgttgttg gtatgcatca ggaacgtggt
     2401 attatctgca gaaagctctt ttacttgtaa agtggcaaag tgtttcccgt cccccagttc
     2461 aattcgcgag cccttagcct tcagtgcacc ggcctctgat tttccgtcag agaaccacag
     2521 agcattgatc atatttaccg tactctgtgc ccccgtaata ctgcctctca gaacagcgtt
     2581 atatcccttc attaaggagc tgaataacgg agagtccgtc ttccctgcat cagcatcact
     2641 gtctcccaga ttaatggttg ccgcagtggt tcctgcatga atatccgaac tcagatagcc
     2701 catattttta acagaaaagc tggcgttatc ttcgaggtta atcccttctg ttgtagaaat
     2761 tacgggtgag taatacccct gtttctgcgc agaaggcatc cccgtcaggg tcagagaagc
     2821 attttctttt acatccacag ccccggtgct gaaaataccg ctctgagccg taagttttgc
     2881 tccttttccg agggtcaggc tgctgttgac aaagctgcca acacggtcaa aaacagcatt
     2941 ctgagaggtc acactcacgg tgctatcata agcctcaata ccaccggtga atctctccat
     3001 gatttgcagg gatgatttat tttccagcgt aacatttccg ctgtaatcac tctggtcacc
     3061 aactgtggtt gcagtggcac tcccttcaac agaggagacc gcattaccgg ttccgtcact
     3121 cctgtcagta aagacacggc tacttcccag aatgactgta cttccattgg tggcgttaat
     3181 atctccctgc atcttgccgt ttctggacag atggaatgtt gccgcatcga gtttcagttt
     3241 ttccagcgta aactgacggg tttcccagtc gctctggctg gaagaagtcg tcgttccagc
     3301 atgaataact ggatgcccct ggaatatcag cgtgccggac ttcttggtta tcgaaccttc
     3361 tggcaggtta actgaaccat caagagccag tacatcctta cctgagagct gcggaatatt
     3421 cacatccata ttgccgttta gttggccgtg atacatataa ggctggctgc tggcctcaat
     3481 tttttgttgc ttcacagtat caatggcttt attacgatct tttccgacat tctgccagac
     3541 actgctgttg ttcagatcgg agaagaacgg ggaatatgaa ctcgctttca ggatgaaata
     3601 ttgcttggta gaactgtcat aatataggtc tcccggcgct cctctccccc caaaaatact
     3661 gacagtatta accggtacat tgatgtcact ggcttttaat gtctgaaggt cgagtgttac
     3721 cgttgatttt ttctcactgt tattggtaat gatggccccg tagtcagccg cctgaagacg
     3781 ggtaaaggtc agattattac cattcagttc cagacgtcct ccacggtatc cccacgaaat
     3841 attatccgga ttaacttgct gtgagtcaga aagcacaact gtcggacgac cgctggcaat
     3901 accaacagag ctgaaagcct gtacctttcc gtcagcatcc gcctgctggt tgagaataac
     3961 ggtaccgtcc cccaccttga gtccgcctgc attcacaccg gtcccgttta ctgtcagggt
     4021 accttcgcca gttttatgca ggttatcacc ggcaactccg ttcacctgcc acagaacgtg
     4081 ggttccttta tcggtgataa tcccgccccc catccatgtc tggccgttca gggcggacac
     4141 cctgtaatcg ccggcaaact gcagataccc agctccctga ttaacggaat tctgcagaac
     4201 gacctcgcca ttattgccgg tgaacaggag gttttttcct gcgttcagat catttccttt
     4261 ctttccatgc atatcccaag tcgtgtttcc ctgagtcagt gtgccggtgc cattagccgc
     4321 atcatatttc cactgcagta ctccttcacc agaggtgtag gctatagttt tgtcaaaatc
     4381 attttgcggt tgctgaccaa ggaaatcctg agtggtgacg acccagttat ttccatagtt
     4441 agttcctgag gaaagcacgc caatcagtac ccattttttt tgcagggaat cataagcaaa
     4501 caggggggag ccgctgtccc ccatattcag ataattagcc agaggtccat tcgcgggatt
     4561 aaaaatgtca ccggtctgag ctgtaatcat ctgtccgttg ttataggagt tcagcgcgct
     4621 gacggttccc ccaacaagaa atccgccatt ggtatacact tgggtgcgat taccattttt
     4681 gtctttgacg tactgcatcc cccccccaag gcgatagaat tcagtaaagc gtccgccggc
     4741 ctgataggca ccgctcacag ccccgatgtc agacacttct gccggcgcca cctctgtcac
     4801 cagtttactc aggcggcggg ttttaatatc aagaccgcta ttattatttg taccaaccgc
     4861 agtataggtg tttttggcgt atccgaagct catcgtgtca gaaccgctga catgtttggc
     4921 ggtaaccaca tactgagggg agtaaagtgt atggtctccc ggaggcaggc taccggtagt
     4981 aatagtggca ctactgaaat cagccatcgg ggctttatca agtttaccga ccagattgcc
     5041 ctgcttgtcg taaatggaaa tattcgtggt ccccgacgta aactgacctt tattctcggc
     5101 aaagtcgcgg aatatctgat aagggatctc tgcactgacc gttgctgaca gagctggcca
     5161 tgccgaagaa agtgataaag ctgcaacaga cgtaagaata acccgacgtg aaagtctgcg
     5221 atggcttttg caggtaaccc tgcgggccag ttcggagaca gcaatcaggc ttttggttat
     5281 atggcaatac ttaagataat atattttgtt catatagaaa catcaaccta attttgacaa
     5341 agattaatta cataggatgt agatggagca gtccgagatt ctactaatat caattattaa
     5401 gcatctgttt tttagttggt gtaatataat ttttattaag gtaagcggaa aaacaaccaa
     5461 aaaaaacgca agatgttatt tttttatcat tttgagccag tttggctggg gggatgctct
     5521 tctcttaagc ttcttgctca actttaaaaa tagttatgcc agttcggtat cgataagggc
     5581 cagtttggat atcg
//
LOCUS       DAJTXY010000187         5555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTXY010000187 DAJTXY010000000
VERSION     DAJTXY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.189"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..639
                     /locus_tag="OO566_003916"
     CDS             <1..639
                     /locus_tag="OO566_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR6606993.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIVGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(693..1298)
                     /gene="azoR"
                     /locus_tag="OO566_003917"
     CDS             complement(693..1298)
                     /gene="azoR"
                     /locus_tag="OO566_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6606994.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1499..5401
                     /gene="hrpA"
                     /locus_tag="OO566_003918"
     CDS             1499..5401
                     /gene="hrpA"
                     /locus_tag="OO566_003918"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6606995.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLSFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1356 c         1541 g         1281 t
ORIGIN      
        1 gtaggcgcgt gcattgtgtt gatggagcta atgatgatgg ttcagttatg gtggtcagtc
       61 tggttatgtt ggccagtatt atcgctactc attgttggcc gtgggtgcgg tgggcttggc
      121 gcgataacaa cagggaaaga tagtcagggg aaactcccgc ccgccgttta ctggctgaca
      181 ttgccctggc gcatcgggat gtggctatct atgcgttggt tttgtcgtcg cctggagccg
      241 gtgagcaaaa tgactgctgg tgtttattta agggcgtttc cacgacatat tccggcacag
      301 aatgcggttc tggacgtcac ctttgaattc cctcgtggac gagcgacaaa agatcgactc
      361 tatttctgtg taccgatgtt ggatctggtg gttccggaag agggggagct ccgacaggcc
      421 gtggcgatgc tggaaacatt acgcgaagag caaggcagcg ttctggtcca ttgcgcattg
      481 ggattatcgc gcagtgcgct ggtagtggcg gcatggctgt tatgttacgg acactgtaaa
      541 acagttgatg aagcgattag ctttattcga gccagacgct cgcatattgt gcttaaggaa
      601 gagcacaaag cgatgttgaa attatgggaa aacaggtaag tggattgaga tgtggactga
      661 atatctacag tccacatcaa gaccgtgtcc ggttatgcag caacaatgct gtcgatggct
      721 gcttttgcgt cagactgtgc tttcgctgcc atttccggac cgtatgcgat cccttcggcg
      781 aagacaaatt tcacatcggt aatgccgata aagccgagga acgtggacag atacggcgtc
      841 accaggtccg ttggtccatc tttgtggatc ccaccgcggc tggtaataac gatggctttt
      901 ttacccgtta ccagaccttc cggaccgttc tcggtatagc ggaaagtaac gcctgcgcgt
      961 gcaaccaggt caaaatagtt tttcaactga gttgagatgt tgaagttata cattggtgcc
     1021 gcaataacga taacgtcgtg ggctttcagc tcggcaatca gctcatcgga aagcgccaga
     1081 gcttcctgct gacgcggagt cagcggcgca tcgctcggac gcagagcgcc aaccagttcg
     1141 ccatccagta ccggaatcgg atttgcagct aggtcgcgaa cggtgatttc atcagcggag
     1201 tgcttttcgc gccattgttc aacaaaataa tcggacaact gattagactg agagtaccct
     1261 gccaggatgc tggatttaag aactaatacc ttgctcatgg tgtttcctta tagatgtttg
     1321 aatggacgat gccccgttgc ttgctgacac tttattcaca atcctgccac agagatagcg
     1381 caataaatcg aagtctatgt tcgaatttat tgaacaacgc atagaaagcc gcgatgtggt
     1441 actctatatc tatcatttaa aagaaaatta atcaggcaga ctactgccca ctaacgttat
     1501 gacagaacaa caaaaattga cctttactgc cttgcaacag cggctggatt cgctgatgct
     1561 gcgcgacaga ctgagttttt ctcgccgtct gcattgtgtt aagaaggtta aaaatcctga
     1621 tgcacaacag gccattttcc aggagatggc gaaagagatt gaccaggcgg cagggaaagt
     1681 cctgctgcgt gaagcggcac gaccggaaat cacttatcct gacaatttac cggttagtca
     1741 gaaaaaacag gacatactcg aagcgattcg tgatcaccag gtggtgatcg tcgccgggga
     1801 aaccggctcc ggtaaaacga ctcagctacc gaaaatctgt atggagctgg ggcgcggtat
     1861 taaagggctg atcggtcata cccagccgcg tcgcctggcg gcacgaacgg tggccaaccg
     1921 tattgcggaa gagctgaaaa cggagccggg cggttgcatc ggttacaaag tgcgtttcag
     1981 cgatcacgta agtgataaca cgatggtcaa gctgatgacc gacgggatcc tgctggcgga
     2041 gatccagcaa gaccgcctgc tgatgcagta cgacaccatc attattgacg aagcgcacga
     2101 acgcagcctg aatatcgatt ttctgctcgg ctatttgaaa gagttgctgc cgcggcgtcc
     2161 tgacctaaaa atcattatca cttccgcgac tatcgacccg gaacgctttt cgcgccactt
     2221 taataatgcg ccgattattg aagtctccgg tcggacctat ccggtggaag tgcgctatcg
     2281 cccgattgtt gaagaagccg atgacaccga gcgcgatcag ttgcaggcga tttttgacgc
     2341 cgtagacgaa ctaagccagg aaagccccgg cgacattctg atctttatga gcggcgagcg
     2401 ggaaattcgc gataccgccg atgcgctgaa caagctgaac ttacgccata ccgaaatctt
     2461 gccgctttat gcgcggcttt cgaacagcga acagaaccgc gtgttccagt cgcacagcgg
     2521 acggtgcatt gtgctggcga ccaacgtcgc ggaaacctcg ctgaccgtac cggggattaa
     2581 atacgtcatc gaccccggta cagcgcgtat cagccgctac agctatcgca ccaaagtgca
     2641 gcgtttgccg attgaaccca tttcgcaggc gtctgccaat cagcgtaaag gccgctgtgg
     2701 tcgtgtgtcc gaagggatct gtattcgtct ctattccgaa gacgatttcc tctcgcgccc
     2761 ggagtttacc gatccggaga ttctgcgtac caacctggcc tcggttattt tgcagatgac
     2821 cgcgctgggg ctgggcgata tcgctgcgtt cccgtttgtc gaagcaccgg ataaacgcaa
     2881 tatccaggat ggcgtgcgtc tgctcgaaga actgggcgcg atcaccacgg atgaacaggc
     2941 cagcgcctat aaactgacgc cgctcggtcg ccagctctcg cagttgcctg tcgacccacg
     3001 tctggcgcgt atggtgctgg aagcgcaaaa acatggctgc gtgcgtgagg cgatgattat
     3061 cacgtccgcg ctctccatcc aggatccgcg cgaacgtccg atggacaaac agcaggcatc
     3121 ggacgaaaaa catcgtcgct tccacgacaa agaatccgac ttcctcgcgt ttgtaaatct
     3181 gtggaattat cttggcgagc agcaaaaggc gctttcttcc aacgccttcc gtcgcctgtg
     3241 tcgtaccgat tacctcaact atctgcgcgt gcgcgaatgg caggatatct acacccagtt
     3301 gcgccaggtg gtgaaagaac ttggcattcc ggtcaacagc gaaccggcgg agtatcgtga
     3361 aattcacatc gccttactga ccggtttgct ttcccatatc ggcatgaaag atgccgataa
     3421 acaggaatat accggtgcac gtaacgcgcg tttctccatc ttccccggtt ctggtttatt
     3481 caaaaagccg ccgaaatggg taatggtggc ggaactggta gaaaccagcc gcctgtgggg
     3541 gcgcattgct gcgcgtatcg acccggaatg ggtagaaccc gttgctcagc atttgattaa
     3601 acgcacctac agcgaaccgc actgggaacg ggcgcagggc gcggtgatgg caacggaaaa
     3661 agtcactgtt tatggtttgc cgattgttgc tgcgcgcaag gtcaactaca gccagatcga
     3721 ttcggcgtta tgtcgtgaac tctttattcg ccacgctctg gtggaaggtg actggcagac
     3781 gcgtcacgca ttcttccgtg aaaacctgaa actgcgggcc gaagtggaag agctggaaca
     3841 caaatcacgt cgccgcgata ttctggttga tgacgaaacg ctgtttgagt tctacgacca
     3901 gcgcatcagc cacgatgtaa tctccgctcg tcacttcgat agctggtgga aaaaagtcag
     3961 ccgcgaaacg cctgatttgc tcaactttga aaagagcatg ttgatcaaag aaggggcgga
     4021 aaaaatcagc aagctggatt acccgaactt ctggcatcag ggcaatctca agctgcgttt
     4081 gagctatcag tttgagccag gcgcggacgc ggacggtgtg accgtacata ttccgctgcc
     4141 gttacttaac caggttgagg aaagcgggtt tgaatggcag atccccggtc tgcgccgcga
     4201 attggtgatt gctctgatta aatcgttgcc gaaaccggta cgccgtaatt ttgtgcctgc
     4261 accaaactac gccgaagcgt ttttaggccg cgtcacaccg ctggagttac cgttgctcga
     4321 cagccttgag cgcgagttac ggcggatgac cggcgttacc gttgaccgcg aagactggca
     4381 ctgggatcag gtgcccgatc acctgaaaat caccttccgc gtggtggatg acaaaaacaa
     4441 gaagctaaaa gaagggcgct cgctacaaga tctgaaagat gcgctgaaag gcaaagtgca
     4501 ggaaacgctg tccgcggtgg cggatgacgg tatcgagcag agcggcttac atatctggag
     4561 ttttggtcag ctgccggaaa gttacgaaca gaagcgtggc aactataaag tgaaggcgtg
     4621 gccggcgctg gtggatgaac gcgacagcgt ggcgatcaaa ctgtttgata acccgctgga
     4681 gcaaaaacag gcaatgtgga atggtcttcg ccgtctactg ctgctaaata tttcctcgcc
     4741 gatcaagtat ttacatgaga agttaccaaa caaagccaag ctggggctgt actttaaccc
     4801 gtatggcaaa gtgctggagc tgatcgacga ctgtatctcc tgcggtgtgg atcaactgat
     4861 cgacgccaat gatggcccgg tctggacgga agaaggcttt gctgcgctgc atgaaaaagt
     4921 gcgcgccgaa ctgaatgaca cggtggtgga tattgcgaag caggtcgagc aaatcctcac
     4981 tgcggtgttc aatatcaaca aacgtctgaa agggcgggtg gatatgacca tggcactggg
     5041 gctttctgac attaaagcgc agatgggcgg gttggtatat cgcggttttg tcactggtaa
     5101 cggcttcaaa cgtctgggcg acacgctgcg atatttgcag gcgattgaaa aacgactgga
     5161 aaaactggcg gttgatccac atcgcgatcg tgcacagatg ctgaaagtcg aaaacgtcca
     5221 gcaggcgtgg cagcaatgga tcaacaaact gccgcctgca cgtcgtgagg atgaagacgt
     5281 gaaagagatc cgttggatga tagaagagct gcgcgttagt tacttcgctc aacaacttgg
     5341 tacgccttat ccgatttcag ataagcgtat tttgcaggcg atggagcaga ttagcggtta
     5401 accctgctat ttgcctaata aatctaaaac ccggtaagca tttagcgccg ggttttttat
     5461 taattctaaa acggtaagga caaaaattca ggaattcaga aaaatacaat tctcagctgc
     5521 aagatgaata atgtttatct acagcatttc cttaa
//
LOCUS       DAJTXY010000188         5538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTXY010000188 DAJTXY010000000
VERSION     DAJTXY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.190"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO566_003919"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO566_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6606996.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO566_003920"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO566_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6606997.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>5538)
                     /gene="mdtF"
                     /locus_tag="OO566_003921"
     CDS             complement(2659..>5538)
                     /gene="mdtF"
                     /locus_tag="OO566_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HCR6606998.1"
                     /translation="SSTSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAV
                     QQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGS
                     EYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQT
                     RLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAG
                     ANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLV
                     MYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVV
                     VENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQF
                     SITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDS
                     TRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTT
                     KVLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTA
                     IIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLS
                     LAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGSSYVNDF
                     LNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYN
                     GIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYA
                     ISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIG
                     LSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHG
                     AGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1464 a         1288 c         1302 g         1484 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atatcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaaaaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaaga
//
LOCUS       DAJTXY010000189         5531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTXY010000189 DAJTXY010000000
VERSION     DAJTXY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.191"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..199
                     /locus_tag="OO566_003922"
     CDS             <1..199
                     /locus_tag="OO566_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6606999.1"
                     /translation="IAMLGAGLAAIPEVCNLLFTPTIYLLLNIFMLTLGALMLFFSGR
                     VGNVPEFCYVGYDGVGFAVIP"
     gene            complement(405..1910)
                     /gene="glpD"
                     /locus_tag="OO566_003923"
     CDS             complement(405..1910)
                     /gene="glpD"
                     /locus_tag="OO566_003923"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6607000.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            2100..2426
                     /gene="glpE"
                     /locus_tag="OO566_003924"
     CDS             2100..2426
                     /gene="glpE"
                     /locus_tag="OO566_003924"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6607001.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2471..3301
                     /gene="glpG"
                     /locus_tag="OO566_003925"
     CDS             2471..3301
                     /gene="glpG"
                     /locus_tag="OO566_003925"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6607002.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            3318..4076
                     /locus_tag="OO566_003926"
     CDS             3318..4076
                     /locus_tag="OO566_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6607003.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(4058..>5531)
                     /gene="rtcR"
                     /locus_tag="OO566_003927"
     CDS             complement(4058..>5531)
                     /gene="rtcR"
                     /locus_tag="OO566_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6607004.1"
                     /translation="RLELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFE
                     EVYACLHDFARGYEFQPEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRK
                     KEQPCGPGEVTIIDLDLSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEK
                     VAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLF
                     GHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDR
                     QVSSDFQLIAGTVRDLRQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEV
                     ERHATLTGDSVRFNTEARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLD
                     VVEDEINRLRYNWQESRPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGR
                     QLFDVSRQGKASVNDADRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         1220 a         1466 c         1503 g         1342 t
ORIGIN      
        1 tattgccatg ctgggggctg ggcttgccgc tattcctgag gtttgcaatt tactctttac
       61 gccaacaatt taccttctgc tcaatatttt tatgctgacg ctgggcgcat taatgctatt
      121 tttctcgggg cgagtgggga acgtgcctga gttttgttac gttgggtatg atggcgtggg
      181 ctttgcggta attccctgaa ttaaaaagta ggttagattt ttgacaaagt gcgctttgtt
      241 catgccggat gcgacgtgaa cgtcttatct ggcctacaag accatgcaaa ttcaatatat
      301 tgcataaacc acgtaggcct gataagcgta gcgcatcagg cagttttgtg tttgtcatca
      361 gtctcaggcc agattgaaat ctgacctgat caccttacgt taatttacga cgccagcgac
      421 aacttctgct gcgtatattc caccagccac tgactcacac gagattgttg atccgcattc
      481 agccacatgc cttgttttgt gcgacgccac agggcatcgt cggtgcggcg gacccattcg
      541 tgatccacca ggtatttcag ttccgcttcg tagaactcat gaccgaaatc ttccccgaga
      601 tcgcttaccg ttcccgcatt gccgagcagc agctcgctgt tgctgccgta agtgcgcgcg
      661 tagtgacgtg ccagcgattc agtcaggaac gggtaacggc ggcgcaggcg agcggcgtaa
      721 tcgtcgcggt cgccttcaat ggcaccaccc ggcagcacgc tctctttcgt ccacgccggg
      781 ccaataccct gataatacgg cgtcagtttt tccagcgcat gttctgccag ttttcggtag
      841 gtggtcagct taccgccgaa taccgacagc agcggtgctt tgccgttttc atcatggata
      901 tcaagggtgt aatcacgggt aatggcttgc ggcgagtcgg actcatcatc acacagcgga
      961 cgcacaccgg agtaggtcca gacgatatcg tcacggctta gctgcttttt aaagtgcgcg
     1021 ttatacactt tcagcaggta attgatttca ctctcttcaa tcttcactgc tttcggatca
     1081 cctttgtatt ccacatcggt ggtgccgatg atggaaaact cgtccatcca cgggatcacg
     1141 aacacaatgc gtttatcttc gttttgcaga atgtaggcct gcttttgggt atgcacgcgc
     1201 ggcaccacaa tatggctgcc tttgatcagg cgaatgccat aaggcgaagg cagatgcatc
     1261 ccctcgtcga agaactgttt cacccacggg ccggtggcgt taaccaggcc gcgcgcttgc
     1321 cagctatatt ttttgccggt atcgatatct tccgcttcca caatccacag gccgttttcg
     1381 cggcgagcag aggtggcgcg agtccgagta agcacttcgc cgcctttacg caccaccatc
     1441 tgggcgttgg cgagtaccag acgggcgtcg tctacccaac agtcagaata ttcgaatccg
     1501 cgcttaattt ccggttttaa cactgaattt gcgccaaaac gcaaaccaat tgatcccggc
     1561 aggctggtgc gtttacccag atgatcgtac ataaacagac caatgcgaat catccacgcc
     1621 gggcgcagat gcggacgatg tggcaggcga aaacgcatcg ggaaggcgat atgcggagcc
     1681 attttcagca gcacttcacg ttcagccagc gcctcgctga ccaggcggaa ttcatagtgc
     1741 tcaaggtagc gcaggccacc gtgaatgagt tttgaactgg cggaagaggt cgcgcaagcg
     1801 agatcctgcg cctccagcat cagcacggat aaaccgcgtc cagcggcgtc tgccgcgata
     1861 ccagcaccat tgatgccgcc acctatcaca atcagatctt tggtttccat gctgccctca
     1921 ttcactttcg ttaaagctca taaatgttcg ttatcgaaca tattagcaaa gaatcgcgct
     1981 ttgggtaaca ttgaaaaaac attttagagt gatatgtata acattatggc gtttatctgc
     2041 cgcttcgacg taaactgtgc ggtaaatttg cccacttgtt tgtaaagaaa gagagacgca
     2101 tggatcagtt cgaatgtatt aacgttgccg acgcgcacca gaagttgcag gaaaaagagg
     2161 cggtgctggt cgatattcgc gatccacaga gtttcgctat ggggcatgcg gtgcaggctt
     2221 tccatttaac caacgacacg ctgggcgcgt ttatgcgtga taacgacttt gacactccgg
     2281 tgatggtgat gtgttatcac ggcaatagca gcaaaggggc ggcgcagtat ctgctgcaac
     2341 agggctacga tgtggtctat agcattgacg gcggctttga agcctggcag cgacagtttc
     2401 ccgcagaggt ggcgtacggc gcgtaacgct ttatactgtc cccttttgtg tggaataagc
     2461 gacagcaacg atgttgatga ttacctcttt tgctaacccc cgcgtggcgc aggcgtttgt
     2521 tgattacatg gcgacgcagg gtgttatcct cacgattcaa caacataacc aaagcgatgt
     2581 ctggctggcg gatgagtccc aggccgagcg cgtgcgggcg gagctggcgc gttttctcga
     2641 aaacccggca gatccgcgtt atctggcggc cagctggctg gcaggccata ccggcagtgg
     2701 cctgcattat cgccgttatc ctttctttgc tgccttgcgt gaacgcgcag gtccggtgac
     2761 ctgggtgatg atgatcgcct gcgtggtggt gtttattgcc atgcaaattc tcggcgatca
     2821 ggaagtgatg ttatggctgg cctggccatt cgatccaaca ctgaaatttg agttctggcg
     2881 ttacttcacc cacgcgttaa tgcacttctc gctgatgcat atcctcttta acctgctttg
     2941 gtggtggtat ctcggcggcg cggtggaaaa acgcctcggt agcggtaagc taattgtcat
     3001 tacgcttatc agcgccctgt taagcggcta tgtgcagcaa aaattcagcg ggccgtggtt
     3061 tggcgggctt tctggcgtgg tgtatgcgct gatgggctac gtctggctac gtggcgaacg
     3121 cgatccgcaa agtggcattt acctgcaacg tgggttaatt atctttgcgc tgatctggat
     3181 tgtcgccgga tggtttgatt tgtttgggat gtcgatggcg aacggagcac acatcgccgg
     3241 attagccgtg ggtttagcga tggcttttgt tgattcgctc aatgcgcgaa aacgaaaata
     3301 attccaggga tttataaatg aaacaaacac aacgtcacaa cggtattatc gaactggtta
     3361 aacagcaggg ttatgtcagt accgaggaac tggtggagca cttctccgtc agcccgcaga
     3421 ctattcgccg cgacctcaac gagctggcag agcaaaacct gatcctgcgc catcatggcg
     3481 gcgcggcgct gccttccagt tcggttaaca cgccgtggca cgatcgtaaa gccacccaga
     3541 ccgaagaaaa agagcgcatc gcccgcaaag tggcggagca aatccccaat ggctcgacgc
     3601 tgtttatcga tatcggcacc acgccggaag cggtggcgca cgcgctgctc aatcacagca
     3661 atttgcgcat tgtcaccaac aatctcaacg ttgctaacac gttgatggta aaagaagatt
     3721 tccgcattat tctcgccggt ggcgaattac gtagccgcga tggcgggatc attggcgaag
     3781 cgacgctcga ttttatctcc cagttccgcc ttgatttcgg cattctgggg atcagcggca
     3841 tcgacagcga cggctcgctg ctggagttcg attaccacga agttcgtacc aagcgcgcca
     3901 ttattgagaa ctcgcgccac gttatgctgg tggttgacca ttcaaaattt ggccgtaatg
     3961 cgatggtcaa tatggggagc atcagcatgg ttgatgccgt ctacaccgac gccccgccgc
     4021 cagtaagcgt gatgcaggtg ctgacggacc accatattca actggagctg tgctgatcct
     4081 gcacggcctc ccacgtcaga ccaaaacgcg ccaggtattt gcgtagccga tccgcgtcat
     4141 tgacgctggc tttgccctgg cgcgaaacat caaaaagctg tcgtccggca gcagaaagcg
     4201 actttgcctg gcggcagata gcgataacgt gttccagttg catacggtcg aagagatcga
     4261 tgttttctgc ctctgcgccc agcaacgccg taagcgcgga ggggcggctc tcctgccagt
     4321 tatagcgcag acggtttatc tcatcttcca ccacgtccag agtgatgcgt ccgctagtgg
     4381 caaaggtggc catccgcgtg acgctggcag aaagttcgcg aaagttaccg cgccatgtag
     4441 cctggggaga tgttgcaaag gccagccagg cacgccgcgc ttcggtgtta aaacgcacgc
     4501 tgtcaccagt gagtgtggcg tggcgctcca cttcataatc caggttcggt tcaatatctt
     4561 cctggcgctg gcgtagaccc ggcagggtga aggtccagag attgatccgc gcgtacagat
     4621 cttcgcggaa tttgccttcg gcaaccagct ggcgcaaatc gcgcaccgtt ccggcgataa
     4681 gctgaaaatc actgctcacc tggcgatcgc tgccaaacgg gtaaaaggtt ttctcttcaa
     4741 tagctttcag cagcattgcc tgttcgtctg cgcccagttc gccaatctca tcaagaaaca
     4801 acattccgcc gttggcgctg cgtaataatc cttcacgaga ttcccgcgcc ccggtaaacg
     4861 cgcctttcac atgaccaaac agcgtcgaca tcgcggtatc gccgcgcagg gtggcgcagt
     4921 tcacttccac aaacgcgccg ctaaactgat gccgtgcctg ttttaactcg aagatgcgtc
     4981 gcgccagaaa tgacttacct gcgccggtcg ggccgttaag cagaatcggc gcgcgggatt
     5041 taatcgccac tttttcgatc tgctcaatca tgcggttgaa gtgggggtta cgcgtggcaa
     5101 taccggattt gaggaaatcg agcgtttgct gccgttcctc ggcaaagcgg ctggcgatgg
     5161 cgttataacg gcttaaatcg agatcgataa tcgtcacttc acctgggccg cacggctgtt
     5221 cttttttgcg cggagttgaa gattgtatca accgggcggg tagataacgc gcttccgcca
     5281 gcagaaacca gcaaatctgc gcgacgtggg tgccggtggt gatgtgaatt aaatagtctt
     5341 ctttttctgg ctgaaactcg taaccacggg cgaaatcatg caggcaggcg taaacctctt
     5401 cgaaatccca cgggttatgc agttcaatct caacgcccac cacttctgtt tctggcgaaa
     5461 cgctggcgat atcgcgttta agcgtttcaa atagcgagcg cgagcgggca tcgtgcaata
     5521 attccagtcg a
//
LOCUS       DAJTXY010000190         5495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTXY010000190 DAJTXY010000000
VERSION     DAJTXY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.192"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..397
                     /locus_tag="OO566_003928"
     CDS             <1..397
                     /locus_tag="OO566_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6607005.1"
                     /translation="RKNYSNEFKLRMVELASQPGASVARIAREHDINDNLLFKWLRLW
                     QNEGRISRRLQVTTSSDTGVELLPVEITPDEQKKTVAAIAPSLSTSTQTRVSASSCKV
                     EFRHGNMTLENPSPELLTVLIRELSGRGR"
     gene            394..684
                     /gene="tnpB"
                     /locus_tag="OO566_003929"
     CDS             394..684
                     /gene="tnpB"
                     /locus_tag="OO566_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6607006.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAML"
     gene            complement(936..1679)
                     /locus_tag="OO566_003930"
     CDS             complement(936..1679)
                     /locus_tag="OO566_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR6607007.1"
                     /translation="MVKILAVAQSHESIVSMDEINSILLSSSWFRALPDYLIEQMLSR
                     TSLYQYNDGEIIHYRGAEPLGLYAVITGSVKVSSISADGKECIFRYLSPGNWFGEIGM
                     IDKCPRTHDARATGKTTLLTIKPHDVEFLLKNYPILHRFLALLLCKVVRNAFTMLHDC
                     TLLSVSARLAKRLVSLSEGYGEPHERGTLINLYLTQDDLATIINTSRQTINKRLVEWE
                     KLGWIDARYGKIVIININALRQLYADEDE"
     gene            complement(1729..3096)
                     /locus_tag="OO566_003931"
     CDS             complement(1729..3096)
                     /locus_tag="OO566_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6607008.1"
                     /translation="MPSHTDQYLSLAIQKSIRRIIPMVILMFILAYLDRSNIGFAKQE
                     FQLDTSLSDTAYAFGAGIFFIGYALFEVPSNILLYRIGARVWLARIMVTWGLVSAAMM
                     FAHTETVFIFLRFLLGISEAGFFPGVILYLTFWFPQHIRARVTGYFLFGAPLAFIIGG
                     PLSGSLLSLEGSVLSFGLHGWQLMFLVEGLLASAVGIWVFFYLDDHPEKAKWLTPQEK
                     AALSQTLNKEEQVKLAHGPKGAWQALLDVRVLYLCLIWFTVQVCGYGIYFFLPTQIAT
                     LIGTKVGLLVGFVTAIPPLCAAIAVYYVPRISEKLQERRYVASITFMSGAIGIAVSGL
                     ADSIPLVAIVALCVAAAGHLAMQPLYWTFPSSYLGGAAAASGIALINSIGNLGGFVAP
                     NLRVWAEHTFNSAQAGLYLLAMVTATGSLMILTLRHFGIEKTPRTSSTSPNLPASGDN
                     LHHSD"
     gene            complement(3168..4280)
                     /locus_tag="OO566_003932"
     CDS             complement(3168..4280)
                     /locus_tag="OO566_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HCR6607009.1"
                     /translation="MKITKLTTFIMHVPVTKNIIGDSTHSITHWGMPGVMIETDTGLV
                     GYGYTGTHADITTDRLITTIIKDAFGPMLIGEDASEVRYLHRKMSRSSTNIWVGRGGL
                     MQMALSAIDIALWDLKAKMVEQPLWKVFGGSQHSRVLAYNTDCGWLVRNTAELVDDCK
                     KMIFEEGFSAIKMKIGKPDPREDLKRIEAVRKAIGDDVNLMVDANAKWDISTAKQYGA
                     KLADFDITWFEEPMWHDDIASHRTLSGHISTPIALGELLYQFDSFKEFVLAGAVDYLQ
                     PDATRCGGLTAVWEIADLGQAFNLPVTPHHGDMMQAQLHLVMAHPSCSLLEFIPWTLG
                     CFVDPVEVKEGYYSLPTAPGAGTTLRPEALEHFSVR"
     gene            4446..4927
                     /locus_tag="OO566_003933"
                     /pseudo
     CDS             4446..4927
                     /locus_tag="OO566_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
     gene            4988..5421
                     /locus_tag="OO566_003934"
                     /pseudo
     CDS             4988..5421
                     /locus_tag="OO566_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1539 a         1320 c         1329 g         1307 t
ORIGIN      
        1 tcgtaaaaac tattcgaatg aattcaaact tcgtatggtg gaactggcat cacaaccagg
       61 agcctctgtt gcccgtattg cccgggaaca cgatatcaat gataacctcc tgttcaaatg
      121 gctcaggctc tggcagaacg aagggcgtat atcacggcgt cttcaggtaa cgacctcttc
      181 tgacactggc gttgaattat tacctgtaga aataacgccg gatgagcaga aaaaaactgt
      241 ggcggccatt gcgccgtctt tatccacttc cactcagacc agagtcagtg ccagttcctg
      301 caaggtggag ttccgtcacg gtaacatgac gctggaaaat ccatcgccag agctgctcac
      361 ggtgttgatc cgcgaactga gcgggagggg aagatgattt cactcccatc aggtacccgt
      421 atctggctcg ttgccggcgt taccgatatg cgtaaatcct tcaacggact gggagaacag
      481 gtacaacatg tgctgaatga taatcccttc tccggccacc tgtttatctt ccgtggccga
      541 cggggtgaca ccgttaaaat tctttgggct gatgctgatg gtctgtgcct gttcaccaaa
      601 cgccttgagg aaggtcagtt tatctggcct gcggtgcgtg acggtaagat atccattacc
      661 cgctcgcaac tggcaatgct gtaaacgtta acggagcgcc gtattgacgc ttacgaactg
      721 tgtgatccga catgccatta ggaaacgtaa ggcgttcccg atgggcgagt cagtaaaagt
      781 gaaatggctg gcatcctggt cgcgtcgcag aaatggacaa tgccgcagag ggactggcga
      841 atagcaattt attatcgaat ttgatgaccg cctggacagt cacttttaag aaaaagcatg
      901 tatacagaat ggtaaacagg ctcatccaca gccaattact catcttcatc agcatataac
      961 tgcctgagtg catttatatt gatgatcacg attttgccat atctggcgtc tatccatccc
     1021 aatttttccc attcaaccag ccgtttgttg atcgtttgtc ggctcgtatt gattatcgtt
     1081 gccagatcgt cctgcgtaag ataaagattg attaatgtcc ctcgctcgtg tggttcgccg
     1141 tacccttccg acagactcac taaacgcttc gccaagcgtg cagaaacaga taatagcgta
     1201 cagtcatgta acatcgtaaa ggcatttcga acgactttgc aaagcagcaa cgccagaaaa
     1261 cgatggagaa ttggataatt tttcagaaga aattcaacat catgaggctt gatcgttaac
     1321 agtgtagttt tacccgtcgc tctggcatcg tgtgtgcgcg gacatttatc aatcatacct
     1381 atttcaccaa accagttccc cggcgaaaga tagcggaaga tgcactcttt accgtcagca
     1441 gaaattgagc tcactttaac ggagccagta atgactgcat ataatccgag cggctcagca
     1501 ccacgatagt gaataatctc tccatcgttg tattgataca ggctggtcct ggacaacatc
     1561 tgttcaataa gataatcagg tagcgcccga aaccaactac tactaagaag gatactattg
     1621 atctcgtcca tagaaactat cgattcatgt gattgtgcta cggccaatat cttaaccatt
     1681 gttgttatcc tgatcttaat catgaccgta gcaatgtagc atttcctgtc aatcagagtg
     1741 atgcagatta tccccgctgg cagggagatt cggagatgtg gatgatgtac ggggcgtttt
     1801 ttcaatcccg aagtgacgta atgtcagaat catcaggcta ccagtagctg ttaccattgc
     1861 cagtaagtaa agtcctgcct gtgcggaatt aaaagtatgt tccgcccaga cgcgaagatt
     1921 cggcgctaca aaccccccca gattgcctat cgagttaatt aacgcaatgc cggaagcagc
     1981 tgctgcaccg ccgagataag acgatggaaa tgtccagtac agtggctgca tcgccagatg
     2041 tcctgcagcg gcaacgcaca gcgcgacaat agcgaccagt ggaatactat cggcaaggcc
     2101 agatacggca atgcctattg caccggacat aaatgtgatt gatgcaacgt aacgacgttc
     2161 ctgcagtttt tccgatatac gtggtacgta atacactgcg atggcagcgc acaaaggtgg
     2221 aatagcggtg acgaagccaa ctaataaacc aacttttgtc cctattaagg tggcgatttg
     2281 tgttggcaga aagaagtaaa ttccataacc acatacctgc accgtaaacc agatcaggca
     2341 taaatagagg acgcgtacat ccagcagtgc ttgccatgcg ccttttggac catgtgcaag
     2401 tttcacctgc tcttccttat ttagagtctg gctaagcgcc gctttctctt gtggagttaa
     2461 ccatttggct ttctctgggt gatcgtcaag atagaagaac acccagatcc caacggcaga
     2521 ggccagtaac ccctcaacga ggaacatgag ttgccagcca tggagaccaa acgacagtac
     2581 cgatccttca agcgagagta gcgagccgga aagcggaccg ccgatgataa aagctagtgg
     2641 ggcaccaaac aggaaatagc cggtgacgcg tgcgcggatg tgctggggga accagaatgt
     2701 aagataaaga ataactccag gaaagaaacc tgcttcactg atgccaagca agaagcgcag
     2761 gaaaataaat acggtctcag tgtgggcgaa catcattgca gcggaaacca gcccccaggt
     2821 caccatgata cgggccagcc agacacgggc gccgatgcga taaagcagaa tattactggg
     2881 cacctcgaac agggcataac caataaagaa gatcccggcg ccgaaagcat aggcggtgtc
     2941 actcagactg gtatccagtt gaaattcttg tttagcaaag ccgatattgg agcgatccag
     3001 ataggccaga ataaacatta gtatgaccat cggaatgatg cgacgtatgg atttttgaat
     3061 tgcaagtgat aaatactgat cggtatggct gggcataaat cacctcttag aattttgtca
     3121 ttattatttt gccccggtca ttgcataacc gggcgataca agtgttctca tctaacgcta
     3181 aagtgctcaa gagcttccgg gcgtaaggta gtgccggcac ccggcgcggt gggaagagag
     3241 taatatccct ctttgacctc aaccggatcg acaaagcagc ccagcgtcca gggaataaac
     3301 tccaacagag agcaagaggg atgtgccatt accagatgta attgtgcctg catcatatca
     3361 ccatgatgtg gtgtgaccgg cagattgaac gcctgaccga gatcggctat ttcccatacg
     3421 gcggtcagtc caccgcagcg agtggcatcg ggctggagat aatccaccgc accggccagt
     3481 acaaactctt taaaggaatc aaactgatag agtaactcac ccaacgcaat cggcgtactg
     3541 atatgcccgg acagcgtgcg gtgacttgcg atatcatcat gccacatcgg ctcttcaaac
     3601 caggtaatat cgaaatcagc cagcttggca ccgtactgct tggcagtgct gatgtcccat
     3661 tttgcattcg cgtcgaccat cagattgacg tcatcgccta tcgcttttct gacggcttcg
     3721 attcgtttga gatcttctcg cggatcgggc ttgcctatct tcatcttgat ggcggaaaat
     3781 ccctcctcaa aaatcatctt cttgcagtca tccactaact ccgccgtgtt acgaaccagc
     3841 cagccgcagt cggtgttgta ggcaagaaca cgggaatgtt gagaaccgcc aaagactttc
     3901 cacaaaggtt gctccaccat tttggctttc aggtcccaca gggcgatatc gattgctgaa
     3961 agcgccattt gcattaaccc accgcggccc acccagatat tggtcgagct gcgtgacatc
     4021 ttacggtgga gatagcgaac ttcactggcg tcttctccaa tcaacatcgg gccgaaggca
     4081 tctttaataa tggtggtgat aagacggtcg gtagtaatgt ctgcatgggt tccggtataa
     4141 ccgtaaccta caaggccggt atctgtctcg atcattacgc ccggcattcc ccaatgagta
     4201 atgctgtgtg tggagtcacc gataatattt ttagttaccg gtacatgcat aataaaggta
     4261 gtgagtttag tgattttcat tgcagtttcc tgtgtctgta tatcttgatt gatgggcgca
     4321 atgtagcaac cggccttcag cagggctgtc aaatggttga cacatcatca gaagagagaa
     4381 ggaataattg tatttttttg aagtagatct cgaatttgga ccacacccag tcgacaagtt
     4441 ttcatatagg gaactgggcc atgaccggag cttttcagga tgagatctct gtacgcatca
     4501 tgaagaccat gcggctaaag tgatcctgga gtacagcaaa ctggaaaaaa tcgaaaagta
     4561 ttacgaaaaa tacgcaaacg caggcaccaa tgagtggcac atcgatgaaa gtaaaaaagc
     4621 taccgcaacc atgaatgtag ttctgggtga tgaagatagg ttaaaatccc tcgcggaaga
     4681 ttttgccaga cattatgaaa aacgcgtagc cgaaggtttc accgtaaaag gcaagaccat
     4741 gtttgtgcca gccgtgaaat tgcctagggt tctaccgtca ccttgaagct attcgcactg
     4801 cctggtttta agtgaaacaa gtacccgatg gcatcgtcct gactgaacgg gagcagaaaa
     4861 attaccgcct tctgaaatgg tgaaaatggt catgacgcgt ggtaaagatg atgacgaggc
     4921 gctttataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     4981 ttaatttatg gacgaaaaat agttacagac tctggctaac gaactggcca aaaacctcaa
     5041 aacccctgaa gacctcagcc agtttgaccg tctactgaaa aagatcagcg ttgaagccgc
     5101 tctcaacgca gagatgacac accatcctgg gtatggaaaa atcagtccag accaggagct
     5161 aactcccgca acggttattc cacaaagacc gttatcacag gcgacggtcc actggaactg
     5221 cgtactccgc gcgatcgtga cggtagcttc gaaccacaac aggtgaagaa aaatcagccc
     5281 cgtattacca ggatggataa ccagatcctc tcgttgtatg ccaaagggat aaccacccgt
     5341 gagatagccg ccgcgttcaa agacctgtat gacgctgatg tttcaccggc actgatatca
     5401 aaggtcactg atgccgtgat ggtgaaccgc cccggaaatc ctggagacta aactccctga
     5461 gaaagaggta aacaggatga ctaaaaatac acgtt
//
LOCUS       DAJTXY010000191         5465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTXY010000191 DAJTXY010000000
VERSION     DAJTXY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.193"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(296..580)
                     /locus_tag="OO566_003935"
     CDS             complement(296..580)
                     /locus_tag="OO566_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR6607010.1"
                     /translation="MLDMAGLRADILSPVKETGTLLGVVSSQAAELCGLKAGTPVVVG
                     GGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQLPITPFRMPS"
     gene            614..1420
                     /locus_tag="OO566_003936"
                     /pseudo
     CDS             614..1420
                     /locus_tag="OO566_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            1432..2455
                     /gene="lsrB"
                     /locus_tag="OO566_003937"
                     /pseudo
     CDS             1432..2455
                     /gene="lsrB"
                     /locus_tag="OO566_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            2482..3357
                     /gene="lsrF"
                     /locus_tag="OO566_003938"
     CDS             2482..3357
                     /gene="lsrF"
                     /locus_tag="OO566_003938"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR6607011.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PGVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            3381..3671
                     /gene="lsrG"
                     /locus_tag="OO566_003939"
     CDS             3381..3671
                     /gene="lsrG"
                     /locus_tag="OO566_003939"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6607012.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3710..4468
                     /gene="tam"
                     /locus_tag="OO566_003940"
     CDS             3710..4468
                     /gene="tam"
                     /locus_tag="OO566_003940"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6607013.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(4472..5386)
                     /locus_tag="OO566_003941"
     CDS             complement(4472..5386)
                     /locus_tag="OO566_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR6607014.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNELPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         1379 a         1306 c         1457 g         1323 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt
      661 actgtttcaa agtggtgtgc cgatgccgct ggcgatactc ctgaccttac tgctcggcgc
      721 attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat
      781 tacgcttggc acgctgtatc tgtttgccgg aagcgctctg ctgctttccg gtatggccgg
      841 agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct
      901 ggatgtgctg ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg
      961 gctctggctg cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg
     1021 cgtggcgctt tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac
     1081 ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc
     1141 cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa
     1201 tatttatggt ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata
     1261 tttgcagcaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg
     1321 tgcgctgctt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa
     1381 agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt
     1441 catcgcttta agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat
     1501 gtgcaggccg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc
     1561 agcggtggca acggcgcaca acaagcgggt aaagagctga gcgttgatgt gacctacgac
     1621 gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa
     1681 ggttataacg ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa
     1741 acgcgccatg caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg
     1801 ccgctcttac tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat
     1861 ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac
     1921 cgttacggac caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc
     1981 aggctgggaa attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac
     2041 cgcagaagga atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa
     2101 cgccctgccc gctgccgcac aagccgcaga aaacctgaaa aatgacaaag tagcgattgt
     2161 cggattcagt acgccaaatg tgatgcgccc gtatgtagag cgcggcacgg tgaaagaatt
     2221 tggactgtgg gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgcattatt
     2281 gaaaaaagga tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggttga
     2341 agtctcgcca aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact
     2401 gttaccggag cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc
     2461 attacttaac cggagtaagt tatggcagat ttagacgata ttaaagatgg taaagatttt
     2521 cgtaccgatc aaccgcaaca aaatatccct tttaccctga aaggttgcgg tgcgctggat
     2581 tggggaatgc agtcacgctt atcgtggata tttaatccga aaacgggtaa aactgtgatg
     2641 ctggcctttg accatggtta ttttcagggg ccgactaccg gacttgaacg cattgatata
     2701 aatatcgccc cgctgtttga acatgccgat gtattaatgt gtacgcgcgg cattttgcgc
     2761 agcgtagttc ccccggcgac caataagccg ggggtactgc gggcgtcagg tgcgaactct
     2821 attctggcgg aattaagtaa tgaagccgtg gcgttatcga tggatgatgc cgtgcgcctg
     2881 aacagttgcg cggtggcggc gcaggtttat atcggcagcg aatatgaaca tcagtcgatc
     2941 aaaaatatta ttcagctggt cgatgccgga atgaaagtgg gaatgccgac catggccgtg
     3001 actggcgtgg gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc
     3061 gccgctgaaa tgggggcgca aattatcaaa acctattatg tcgaaaaagg ttttgaacgg
     3121 attgttgccg gatgtccggt acccattgtt attgctggcg gtaaaaaatt accggagcgc
     3181 gaggcgctgg aaatgtgctg gcaggctatc gatcagggcg cttctggtgt ggatatgggg
     3241 cgtaatattt tccagtctga ccatccggtg gcgatgatga aagccgtaca ggcggtggtt
     3301 caccataacg aaacggctga tcgggcatat gaactctatc tgagtgaaaa acagtaactg
     3361 cggatttaag gagaagaatt atgcacgtca cactggttga aattaacgtt catgaagaca
     3421 aggttgacga gtttatcgaa gtttttcgcc agaaccacct gggctctgta caggaagaag
     3481 gtaatttgcg cttcgatgtc ttacaggacc cggaagtgaa ttcgcgcttt tatatttacg
     3541 aagcctataa agatgaagac acagtggcat tccataaaac cacgcctcac tacaaaacct
     3601 gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt
     3661 tgatgccgtg aggctacaat tggggtaacg cgctgacggg agtaaaaaaa tgtctgactg
     3721 gaacccctct ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct
     3781 tgccagagtg ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa
     3841 cagcaccgcc ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc
     3901 tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga
     3961 catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca
     4021 atggctgccc gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg
     4081 cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga
     4141 agttgcttgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc
     4201 ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta
     4261 tcaccagatg ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc
     4321 gtggttacag gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat
     4381 gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct
     4441 gtttattgtt gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa
     4501 gaatttttgc tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt
     4561 tgcagatggc atccagcggt aactcattgt tttcagtacc gaacggatct tccagttctt
     4621 ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca
     4681 tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac
     4741 gatgcaaaat cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg
     4801 ccagaaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa
     4861 gctgtccatt gcggcgctga accgccaacc attctcccat tattaacaag atacggttag
     4921 ctggagagtt cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca
     4981 cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca
     5041 gcctggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta
     5101 acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt
     5161 acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg
     5221 gtgcgagggt gaatttgatg cccagatgcg tgtaccaggg cagcatgaat ataacagcaa
     5281 tagaaaagag aaaattgagt agtaagcgcg aggatatctt ggataatact gagccgtgcc
     5341 agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt atcttcaggc
     5401 gtggaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacgc tacttacaaa
     5461 ttacc
//
LOCUS       DAJTXY010000192         5444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTXY010000192 DAJTXY010000000
VERSION     DAJTXY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.194"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..270
                     /locus_tag="OO566_003942"
                     /pseudo
     CDS             1..270
                     /locus_tag="OO566_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            457..694
                     /locus_tag="OO566_003943"
                     /pseudo
     CDS             457..694
                     /locus_tag="OO566_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1115..1693
                     /locus_tag="OO566_003944"
     CDS             1115..1693
                     /locus_tag="OO566_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607015.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1802..2060)
                     /locus_tag="OO566_003945"
                     /pseudo
     CDS             complement(1802..2060)
                     /locus_tag="OO566_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2044..2600
                     /locus_tag="OO566_003946"
                     /pseudo
     CDS             2044..2600
                     /locus_tag="OO566_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2624..3030
                     /locus_tag="OO566_003947"
                     /pseudo
     CDS             2624..3030
                     /locus_tag="OO566_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3614..4330
                     /locus_tag="OO566_003948"
     CDS             3614..4330
                     /locus_tag="OO566_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607016.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSYKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            4882..5286
                     /locus_tag="OO566_003949"
     CDS             4882..5286
                     /locus_tag="OO566_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607017.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
BASE COUNT         1632 a         1019 c         1303 g         1490 t
ORIGIN      
        1 ataaggggtt ccggatggtg atacggggtc ctgagtccgg tgaagcggcg attactggaa
       61 gatgtggtgt acgccataac ggagacagtg agaaaaacgg cgaggcaaat cacaaggaga
      121 gggatgtatc agcagtgact gaaggttgac ccgctgaagt gcgagctgcg cggaggtcag
      181 atgcggttta cggggctgaa gaggggttac cgtctggcag agcaggttat gatgcatgag
      241 ccgctggctc gaatacggtg gtgcggctga gagtcgcata ggggaagttg cgtccatgtt
      301 gaggggaacg gagcaaaaaa ccatcagaga tacattgaat caatcaatgt aagcaatatt
      361 aagtgtctat accgattact tgatgatcag gttgtgttga ataaacgcgc attcttcggt
      421 gcttttttaa tcttttgggg atttaaattc ctatcgatga actgtcgata tgaaaaagcg
      481 agcatcatac tgacgtcaaa caaaggggtc gcagactggg gagaaatgtt cggagatcac
      541 gtgctggcaa cagcgatcct agatcggctg ctacacgctg aatatcaaag gagagagtta
      601 ccggttaaaa gagaaacgta aagctggagt gctgaccaaa aacacaacgc caatcagtga
      661 tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag tatcttagca ggcatgaaaa
      721 tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg aaggagggag aatgccatct
      781 catcacttat atctggcgaa tgaaacacag atggtcggcc ttgcccccaa acacacggcg
      841 gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga aatatgatta aatcagtgtt
      901 atgttcgaac ctgacgtgtt aacagcagta aagtagtctc gcgaaggtgt tcaaacatta
      961 taatgaatgg catcctcata gtgcgcaggg ttatcgcttg ctacggaaat atctgaagcg
     1021 acgtaccagt aatggggaaa atgatagctg gtttctgaaa atatagagga aaatgtgata
     1081 tatatatcct gttaatattt aaggagaata taaaatgaaa gtctcgttta agtctttagg
     1141 ctatattttt catgatatat ataataaaaa acacactatc gatgagttca acgatgtggt
     1201 aagaaaagca gttttatcag gtaaaattaa cgagctgaat gcatgtcata aagtggcaat
     1261 ttttcttgcc gaaaaagaca atgagataac aaagaaagac aaagccaaga taatagatac
     1321 attaactgaa aactattcca ttgaatttca acaattaatg aatattagtg agcgcacgtt
     1381 aaattcatct ttatatatta ctcctggtga aagtggtttt gtcagttttg ttaatcgtga
     1441 aggaaaaata tgtcatactg catatgtaaa atcatcagat aatagcatgg catattacca
     1501 tgcaaatggt agcagcattg acaaatatat tacggatatg tgcggcctta tttgtatgag
     1561 gcatattgag tctactggca ttatttttta tatgcttgat gaaaaagttt tatctgccat
     1621 tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt tgttcagcaa agaacttata
     1681 taaatgtgtt taattttaga aaaactattt ggtaaaatcc aacaagagtc gagaggttcg
     1741 cgttgcttca gcagggcctc agcctgttca accagactca gttgcaggtc gtgacgcaga
     1801 tttagtggtg gtggttgatt gtcagatacc agccgatgat cttgtagtgc atatcatcag
     1861 actttgagaa gcatatggtt ttgcgggtaa gccgcttaag atgtgtccgc aggttcagat
     1921 tatgtcgctc tatccgctga gtgcatttct tgcttataac atgactggtt gaggacaata
     1981 aagctcggta taccggctca ggcatcggtc atgtagaagg caatgctaaa ctggctcagc
     2041 aaactaagag cagcgcgcat ccacttaccc aatgcggttg agaaaatagc cttcgatcgc
     2101 tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa cccgccagaa tgaacatccg
     2161 catcttccta ttgaaagtcg tcgtcagaca aagggaactc gcttcctgtg gcagcacagc
     2221 gataaatgga tgaccgaatc caggcaggaa aagctgatat ggctgcgtgc gcagatgaag
     2281 ctgacgagcc tgtgctgggc gctgaaagaa ctggcaaagg atatctggag cagaccatgg
     2341 agcgaggaaa gacgcaacga ctggcagaga tggctgcggc caacagtgac gtccccatga
     2401 tgaagaatgt ggctaaaacg atagggaaaa ggttgtacgg tatcctgaac gccatgcgcc
     2461 atggcgtatc aaacggaaat gcggaagcac tgaacagcaa gatcaggctg ctgagaataa
     2521 aagccaaggg ctaccgcaac cgggagcgct ttaagctggg agtgatgttc cactatggga
     2581 aactgaatat ggcgttctaa gccttcccac catgatcggg gaagacccag atatgaacag
     2641 gcacattcag agttacttta aagagcttac tgaacgtctg aggtgttaga gccatgactg
     2701 ccgcagcatt gctggcggaa atgcctgaat cgagcagcct ctcgagacgt gaaatcagcg
     2761 cacttgtcgg tgttgctcag gtaaaccggg actcgggaac tctgcgcggt cgacgaacca
     2821 ttttcggtga ttgtgctggg gaagaacagc tctgtacatg gcggcgcttg aggcccccgg
     2881 tttaatctgg tgataaaggc gttttacatg cgcctacttg cagccggaaa cgccaaaaaa
     2941 gtggcactgg ttgcctgtat gcgtaagttg ctgaccatta tgaacgcaat gctcagaaaa
     3001 aacgaagaat ggaatgaatc gtatttatga aaactggatt gtgggccata aatattccaa
     3061 aaagatagcc cgctccgttt attaccggaa cgggcgagag catgacattg tctgttatta
     3121 taatgagact gctgattcta cttattttta atttcaagaa aagcgttttc tacaaagaat
     3181 tatgagattt aatgttttgt ttagacaata ttatcattct gttcaataat gaaagtcttc
     3241 atcaacaaca agtaaaaaca acgtccctct catcgaaccg gacttgcaga tttcccgcat
     3301 ccggctctct tgcaacacat cagaccttca cgttcgggcg gttatgggga aggtccagca
     3361 agctgtatga gccctaactg tttgaatacc agtagattac cagaaacttc tatcgattga
     3421 agacattacc aactagcgtt actaaatgtt gaccaagttt tgttcacttg tctggttttg
     3481 atagtagcat tctgcagagt attttgatat gataaatatg cattaacaat gatattattg
     3541 ttaatttcct agcataaaaa ttaaaagatc aacttttatc tcgaatattt tttacattat
     3601 cagttggttt cttatgaatg acaattcatt gcttagaaat tcttcattat tcatcgccta
     3661 tatgggatgt gtagggtggg tcagtgctta ttcttatggc tggggaacct ctttttacta
     3721 tggatttcca tggtgggttg ttggtgcagg tttggatgat gttgccagaa gtttactcta
     3781 tgctatcata gtaatgggta tactttttac cggttggggg attggtgttt tattttttct
     3841 tcttataaag aaaagaagta aaatacaaga cttgagtttt tttagactat tttttgctat
     3901 taccctactg tttttccctg taatattcga acttttaatt ctcaaacaat atttcatact
     3961 tccattatca ctttcatgca ttattagttc attggttatt tcaataataa ttcgtatata
     4021 tggacggatc ttttctgtat catgtttttc agatatccct tttgttagaa aacaccgcat
     4081 taagttaatt atggctggtt ttttggttta tttttggctt ttttcctttc ttgttggctg
     4141 gtataaacct caattaaaaa aagaatatca gatgctttgt tataataata gttggtatta
     4201 tgttcttgct cgttatgatt cacggctcgt tttgtctagt tcatataaag atgacagtaa
     4261 cagattctta atatttaata cagaacagtc gggattttat gaaatcaatg atgtttatgt
     4321 cagaaagtaa taatcctctc ccacaaagtg aattaaatga atatataatg cgttcgaagt
     4381 atatgtattg agctgtcata ctgagaaaca aaccttaggg cagcacaatt aaagccagaa
     4441 acaactatgc aagaaagtta ctaaataagt gctcggaaca gtaaaacact caggggggac
     4501 tcagtccgga ttatgtaatc agataaattg ccaaacatca caaatcacca gccggactga
     4561 gtgatgccga gcattgcacc aataccccgt gatgaacgat gcctgatgca gaaagctccc
     4621 tataaaacaa agaacaaaaa ctacgaccgg cgattcaccg tcatactgat gctgcatcgt
     4681 ggtgacactg tctgtcactt tcatgttgac tgaacaactc tgctgcgaag aaggtgtacc
     4741 gccggcggga tttgattgtc cggcgtctag cttccacttc cctgatggaa ctgaaagttg
     4801 ttatggccgg tcagcttgcc ctgtccgcag gacatgaggt gctgtgacgg acagcaccag
     4861 taacggaaag tgacaatgaa aatgaatact gcgctgatag ttgcactaat gtgtatgtgg
     4921 tatgcggtac ccgctgctgc aaaagagact ttactggcta tgccacgtaa ttcaacagaa
     4981 cattgctatg cggagattaa tgtgcacgga ccatatgggg tgtatttccg agtggtcccg
     5041 catccccccg ggggaaagag ctgggtcgaa tgtaacagtg actactatta ctctgataaa
     5101 ccaccgggag tacagatact tggaacccgt gcagggtgtc gggtatacgg aatctgcggg
     5161 accacctcga cactgcatgt ggctggaaga ggggttgtgt gtattaaaaa tatatgcagt
     5221 ccacgtggga tgataattca caggatacgg aaacgtcctg tagtggctgt aagcgatgag
     5281 atgtgatctt acccagcaat agtggacacg cggctaagtg agtaaactct cagtcagagg
     5341 tgactcacat gacaaaaaca gtatcaacca gcaaaaaaac acgtaaacag cattcgcctg
     5401 aattttgcag tgaagccctg aagcttgctg aacgcatcgg tgtt
//
LOCUS       DAJTXY010000193         5425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTXY010000193 DAJTXY010000000
VERSION     DAJTXY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.195"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..87)
                     /locus_tag="OO566_003950"
     CDS             complement(<1..87)
                     /locus_tag="OO566_003950"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6607018.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(249..3557)
                     /gene="icsA"
                     /locus_tag="OO566_003951"
                     /gene_synonym="virG"
     CDS             complement(249..3557)
                     /gene="icsA"
                     /locus_tag="OO566_003951"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6607019.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGSS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4086..5288
                     /gene="virA"
                     /locus_tag="OO566_003952"
     CDS             4086..5288
                     /gene="virA"
                     /locus_tag="OO566_003952"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6607020.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1683 a         1223 c          899 g         1620 t
ORIGIN      
        1 tgcattagtt tctgcaatac cagtaatctg aggcgcataa ccacgaatat ttggcggtag
       61 cattcgttca tcgctttcta atgcaattcc tgtgtaacgc caagcaacag cgcattctgg
      121 cgcatgatct ggtaaacacg tttggcattg atcgcaggca taccatcatg tgcacaaaaa
      181 cccaccgagc ttttacacgg tgggtcccag agaaatgcag gacatcaaca cgccctgcat
      241 ttttattatc agaaggtata tttcacaccc aaaatacctt gggtatctct gtaactgtta
      301 ttacccaatt gctgagatac gtttccccag atgcttaggt tatctgcaag acgccccccc
      361 acaccagttt tgagttcaat cacattacgg ttgcctatct ggtgatttga catgccattc
      421 ataattacac catattgctt tgagctccat ttccaattca cttccaaaaa tggttcgaac
      481 ttacgaagag tctccttatc gatacaactc tgactaatta aggatgtacg caatcccaat
      541 tttgcttgaa tattattgac cccagaggat gtgaccatag tgccattgtg ttcaatatag
      601 tcgtttgctt ttaccccatg cctagttatc tggacttgtg gattgagata caatgcgttg
      661 tcaatattat tatgagcagt ccagcgtatg gttgggtaga tatagccagc ttccaaagct
      721 cctgttattc ctttgctgga atatttctca ccagttaacc catctccttt gactgtatta
      781 ttaaaccaac tgtactgcaa ccaagcatcc atatatagac ctgttcgttc tttttcatcc
      841 tgaaaccaag aactgtacaa accggcagta taaccgctca cagttcctcg tgaactataa
      901 ctagtatact tattatgagt tttgctatcc tgatatccat aagctcccat aatacccaca
      961 tgcaagccat catgatcagt gaagtttgtc ttcaaaatat ctccaccaat ctgataaacc
     1021 atcctgttag ctgtggtttt taattgtcta tcagctaaat tatgctcatt atgtccacca
     1081 acagtacgaa tccacatggt ggtttcgctg agctgacctg tagtaggatc aacaatagtg
     1141 ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt tcattgctat caggttagca
     1201 atataactgc cagcttcagg gcgccatgtg tgaatactac cttcacccaa attaagtgaa
     1261 gatacaagct gtgaactagc tcggcgacta ctcatttgag tagactcttg attgctcatc
     1321 tgttttgatt cttgattatc catctgacta gttagatacc acttattggt attcagtcca
     1381 gatacagtgc cctgtttcag gcgatagtca taactaccag caacaatacg gcctttctga
     1441 ataaaagcat tcttatcaga agaatctgtc gaaataattt gaacaccttc aagagtttta
     1501 tctccagagc ctccctcatt tgtcactgca acataggtaa taccagacgc atggcctgca
     1561 atttccagcc ggtcagtttt actgttgtca ttgccgagaa ccgcgcccaa agataatatt
     1621 ccgccctttc catgccaatt acctttctga acataggtct gaccgacatt tgacgaacta
     1681 ttattgagaa taacatgacc gtttttctcc agagtcatgt tacctgtgac aatgacgggg
     1741 tcaggcaaaa aaggggcatt aatattattg attagcacag tcccactatt taataaagtg
     1801 ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac ctttattaac aataacacca
     1861 gcggtacgtg tcatagcttc aactgtcccc atttttagaa tgcctgcatt gatgttggtg
     1921 aaaccagagt aggtattttt ttccgccaaa atgagagttc cctcccctga ttttgttagg
     1981 gattttccgt cccagctatt tatattatga tggctgagat tatctgccag aatagtacta
     2041 acagtaagtt cagcgttttc tttcagattg aactctccat gactgtcgcc atctgtatca
     2101 ttccagctca aaccgtagat caatttattc tggtcactga tatagctttt tgtatagtca
     2161 atagcaccag tagtgagtaa cggattgatg atagatatat tagaaacact ggaacctgtt
     2221 atccctttat ctgcacttag cagtttatag tccttctgcg cccagttatt tataatagct
     2281 tccactttta cattgttgcc aaatgacata tttccagaag cttccagact actgattcca
     2341 gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac ctgaaacagt gaatttgtta
     2401 cctgatagtg aaacagttgt acaatcacca gcacataaat caccttcaat agttggtgtg
     2461 gtcccagtgt aatcattgtt gataattttc agaatggatg aattgttaac ctgtacatca
     2521 ccagtaatga cagaaccttc atggagtatt aatgaattgt ttccaccagt gatgtggata
     2581 gcattacctg ctacattttg tgttccatca tcttctttac ctgaaattat atatccgcta
     2641 ttagcaatgg tcatattgct accatttata gcatcccccc aatgtccacc attaccgcct
     2701 aaaatagctc cattgttgat tacagacagg ttatctcctg tgatagcatt accaccgtag
     2761 ccatcataac cataaccgta agcagcacct ccgttgcctc cagcgaacgt gcccttattg
     2821 attacagaga ggctgcttcc tgtgatggca tcacctccat taccgccatc accctcacca
     2881 taattgttac ctccattacc accggaaata gtccctttgt tgattatggg tagattgact
     2941 cctgtgacgg catcacctcc attcccccca tcactatcac tataactatc gccaccatgt
     3001 ccacctgaaa taatttctcc atttattata aacagattgt ctcctgtaac agcctcacca
     3061 ccatcaccgt tatggtcagc accgctaccg ccgctaccac caagaatcat gccttgattg
     3121 attatagaca agtcagatcc ggtaatagag tcaccaccat taccgccaca actattacca
     3181 tcattattat caccagcacc accatgacct gcgctaatca taatatcaga gttaatccgt
     3241 agcttagttc tagatgcatg agaggggttg gaagaagtta tataccacgc atccattcca
     3301 tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg ttaataattt ttcataattg
     3361 tcctctgaaa aatgaagttc ttgagtaccc gaaagaggag tagcaaaagc tattggcccc
     3421 ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag acaatttgca tgttttttcc
     3481 tttaccgtag gtaattctcc ggcccccccc tgtgaacatt gggtcatatt acaaaaaaat
     3541 ttgtgaattt gattcatgca ctatattatc agtaagtggt tgataaaccc ctgaaaagat
     3601 tccgttttat ccggaataaa gggacgatat atgcaaaaac atattaaaca aagcctcaac
     3661 caaaacatga gagctgtgtt atatatcaat gcaacgggag aaatttacct gtgtcttgct
     3721 ttgaaaaaaa gaggggaaaa gctatatgtt cgataataaa ttaataattt tactatgata
     3781 gtctgcttca gaccgcaact tttcagtcct ttctgtacgc ttgcccaaag tttaaccact
     3841 ttgtttttct atacacccaa tggttctatc tgccccttga tgttaatccc caaagccaat
     3901 aacatgaatg tattcatatc acaactttcc taccagactc tccaatgcaa taactgcata
     3961 attgttttta gtggaaatgt atcgttttct taaagagaag aataacattc cattttatta
     4021 tgttcccaat ttaattatat taatatatat tggcattaat aggaaaatac atcaggagaa
     4081 atcaaatgca gacatcaaac ataactaacc atgaaagaaa tgactcatca tggatgtcaa
     4141 ctgtcaaatc aactacagaa gtaagttgga ataaattgag tttttgtgac gttttactga
     4201 aaataataac tttcggcata tattctccac acgaaactct agccgaaaaa tatagcgaaa
     4261 aaaaactaat ggatagtttc tcaccatcac tatcacaaga taaaatggac ggagagtttg
     4321 cccacgcaaa catagatggc atttcaatac ggctatgcct taacaaaggc atatgttcgg
     4381 ttttttatct tgatggagat aaaatacaat ccactcaact ttcatcaaaa gaatataata
     4441 acttgttaag ttcactcccc cccaaacaat tcaacttagg aaaagtacat actattacag
     4501 ctccggtatc agggaatttt aaaactcaca agcctgcacc agaagttatt gaaacagcca
     4561 ttaattgctg tacatcgata ataccaaacg atgattactt ccacgttaaa gacactgatt
     4621 ttaatagtgt ttggcatgat atttacagag atattcgagc atctgacagc aatagtacaa
     4681 aaatatattt caacaatatt gaaataccac taaaattaat tgcagatcta attaatgagt
     4741 taggaattaa tgaattcatt gatagcaaaa aagagcttca aatgctaagt tacaaccaag
     4801 taaataaaat aatcaattca aacttccccc aacaagacct atgttttcaa acagaaaaac
     4861 ttttgtttac ttcactcttt caagaccctg catttatatc tgcactaaca tctgcattct
     4921 ggcaatctct tcacatcacg tcttcctctg tggaacacat atatgcccaa attatgtccg
     4981 aaaacataga aaacaggctt aatttcatgc ctgaacaacg agttattaac aattgtggac
     5041 atattataaa aatcaacgca gttgtcccta aaaacgacac tgcaatctct gcctctgggg
     5101 ggagggctta cgaagttagc tcatcaatat taccatcgca cataacatgt aatggtgttg
     5161 gtattaataa aatagaaaca agctatctcg ttcatgctgg aacacttcca agcagtgaag
     5221 ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc atttgccatc atatctcctg
     5281 atgtttaagc caatattata ttggctgcca gactgtaaaa aaacgttctt cagaacagct
     5341 ctataaacta ttaaaccaaa gctaaaagga tggggaccaa tagaatctca gcccatcctt
     5401 tttatatcat tatgaaccgc cccgg
//
LOCUS       DAJTXY010000194         5412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTXY010000194 DAJTXY010000000
VERSION     DAJTXY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.196"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..133)
                     /locus_tag="OO566_003953"
     CDS             complement(<1..133)
                     /locus_tag="OO566_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6607021.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     "
     gene            complement(144..377)
                     /locus_tag="OO566_003954"
     CDS             complement(144..377)
                     /locus_tag="OO566_003954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607022.1"
                     /translation="MSKEYTLSVPPFSLNTLGVDMYNNSSYIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(374..1645)
                     /locus_tag="OO566_003955"
     CDS             complement(374..1645)
                     /locus_tag="OO566_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6607023.1"
                     /translation="MWSGKVILLSLGLSLASFCHASTDIDQLYERIVNKDQTALKELK
                     KLADTNNTQALVLLGFIYEHGVTVSVDIPKAIQWYEKACNQGGEYGCYNARYFFQYGK
                     GVTLDVTRAQKYASKSKTDDLNIPPDLMNKIINQAYELKSGAETDKTKRASLIEFVSR
                     YVGQAPENDLLFWGRIGFSKPEILRLATLWAQEGDPEMDYIVGNLYLDDFSPSDDRRS
                     LDTIKKALQWFRTAAEKGYPMAQYTMGDTYENGSAGLKIDAVEAKKWYELAANKNDEK
                     ALVALGNIYYSGLTGEVDYSKASMLFDKAEQQGAKKAALWLSWMYYNGLGETLDCDKV
                     WDYYEKGAGKYSMKFNKDEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPK
                     ICDVSIEINADKISSITNLQLTLELKGGGRE"
     gene            2039..3409
                     /locus_tag="OO566_003956"
     CDS             2039..3409
                     /locus_tag="OO566_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6607024.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            3591..5412
                     /locus_tag="OO566_003957"
                     /pseudo
     CDS             3591..5412
                     /locus_tag="OO566_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
BASE COUNT         1671 a         1128 c         1045 g         1568 t
ORIGIN      
        1 gcagtgtatt tataacatcg ggagcagatt taccaccgta tatgctgtca tacaattcat
       61 gcgctttctt acttgccacc acgcctgatg aaactatcag cattaaagaa aataatattc
      121 tattaggcaa cataaaatat ctcttattta tcttgtacct gaatatgctc ttcttttaat
      181 aaatcacgtt gttttccatt cagtatcgct gtcgcagatt taatagaata agtcaaatca
      241 tgaaaatcgc ataaattacg attatacatt gggatgtcta tatagctttt tcttatataa
      301 ctggaattat tatacatatc cacaccgagc gtattcaaac taaaaggagg gacagaaaga
      361 gtatattctt tactcactcc ctcccccccc cttttagctc aagcgtcaac tgcaaattag
      421 ttatactact aattttatct gcattaattt caatactgac atcgcatatt ttgggttttt
      481 catttgcgtt aaagccatag gtcccgtaat gttttaacgt gatgtaaggt aaagtatcac
      541 cctgtgtttc tcgggttttc tgatcttgat tacaacgggt taaatattca tccttattga
      601 atttcattga atatttcccc gcaccttttt cgtaataatc ccatacctta tcacaatcaa
      661 gcgtttcacc taatccgttg taatacatcc aacttaacca caatgcggct ttttttgccc
      721 cttgctgttc agccttgtca aaaagcatgc tcgctttaga ataatcaacc tcaccggtca
      781 aaccagaata gtatatattc cccagggcca ctaatgcttt ttcgtcattc ttatttgcag
      841 ctaattcgta ccattttttg gcttcgacag catcaatctt taaaccagca ctgccatttt
      901 cgtaggtatc gcccatggta tactgcgcca tcggataacc tttttctgcc gcagtacgaa
      961 accattgcag tgctttcttt atggtgtcta aagaacgtct atcatcagaa ggtgaaaaat
     1021 catcgagata aagatttccg acaatataat ccatttcggg gtcgccttcc tgcgcccata
     1081 gagtcgccag acgcagaatt tcaggtttac tgaaaccaat tcgcccccaa aaaagcaaat
     1141 cattctcggg tgcctgaccg acataacgac tgacaaattc aattaaactc gcccgtttcg
     1201 ttttgtctgt ctcagccccg gatttcaatt cataggcctg attgattatt ttattcatca
     1261 aatcaggcgg aatattaaga tcgtcagttt tcgacttact ggcatacttc tgtgctcgtg
     1321 tcacatctag ggtgacgcct ttaccatatt gaaagaaata acgtgcatta taacaaccgt
     1381 attcgccccc ctggttacag gctttctcat accactgaat cgcctttggg atgtcaacag
     1441 atactgtcac tccgtgttca taaataaatc ccagtagcac tagcgcctgc gtgttattgg
     1501 tatcagctaa ttttttcagt tcttttaacg ccgtctgatc tttattgaca atacgttcat
     1561 acagttgatc aatatctgtt gaagcatgac aaaaagaagc cagagataaa cccaatgata
     1621 ataaaataac tttaccagac cacatacatt cctccttgat atattcaacc accaaacatc
     1681 cacctcatga ataaatacgc tgccataacg gcccaggtaa taagtaaaca tttaagctga
     1741 aacagcataa ccagtgcctc caacgcttct atttattgcg ataaaataac accatccttt
     1801 gataaaaagt cacacgccca tcaataaatc caaaaataat gtcattagtt gcaggataaa
     1861 atcatttgag cttttcattt gttatattta acgcatgatg tattaatggc aagaatatga
     1921 gagaaactca tacctgacgt aaaaatcact ctcttatgta atatttactt attaacactc
     1981 tgtttaccta acatgcgctc cgcactacac tatgtgggca attttttaag aggtggtaat
     2041 gaacgattat aaaatgacgc caggtgagcg gcgcgcgacc tggggtttag ggaccgtatt
     2101 ttcattgcgc atgctgggca tgtttattgt actgccggtt ctgactacgt acggtatggc
     2161 actgcaaggc gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgaccca
     2221 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgaa aaccattaat
     2281 tgtcggtggg ctggcagtgt ttgccgccgg tagcgttatc gctgcgcttt ctgactccat
     2341 ctggggaatt attctgggcc gggcgctaca aggctccggt gcgattgccg ctgccgttat
     2401 ggcgctgctt tccgatctca cgcgcgaaca aaaccgtacc aaagcaatgg cgtttatcgg
     2461 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
     2521 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacgaccg gcattgcgtt
     2581 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
     2641 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
     2701 tatgtgtctg catattttgc tgatgtcgac gtttgttgcc ctgcccggac aactggctga
     2761 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacaatgc taatcgcctt
     2821 tggctcggtc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
     2881 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cgcaaacgca
     2941 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     3001 cctgctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     3061 tgtttactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     3121 tgacggcatg tttgacggtc agcgggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     3181 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     3241 cccagaaaac gtaatggcag atgaaaaact ggaaaaatgc ttactgacaa cgcctggtgt
     3301 caaagatgta ctgctggcga agttagaaca ttgcgtctat gtgaaagtag acagtaaaat
     3361 caccaatcgt tttgaaatag agcgagtaat caacgaagta tcttattaaa taataagcac
     3421 ggtgctattc atgaaaaata aaatagcacc gtacttcgta acaccataat cctttaaacc
     3481 ctccattatt aatacttcgc ttaattccat ataactcgac gattacatta gaagtaaact
     3541 atacttttta tgaaatatca gggataaatt attcacatgg aaaatacatc atgtacaaat
     3601 cattatttac tattatcatt ctactgtata gcgctagttc atatgcaaaa agcgaatccc
     3661 ctcccgcaag cactataact ttcattgcac aaatcgatca tgttgagcta aataaaacag
     3721 cattatctca tgatatgaaa ctcgttcttg ccgatcgttt ttatttcaaa accagtaatc
     3781 cttgcgaaac aaattcaatt tctgctcgac cagaatcaat ggatattact gacaaagcct
     3841 atgccaacaa attaaaaaaa ttagaaactc cttttcttga tgatcgctat ttctttatga
     3901 ccatcaatca gtgtgacggt aatggcactc ccatgctgac agacataaaa gtatgtacaa
     3961 cagaattatg tggtgctgaa tatacaaaga aaatgtctac tgtatggctc gatgaaactt
     4021 taaaaccaac tgctaaacgt ctggcaattt attttattca atctccttta ccctatgacg
     4081 aggagaaaaa acaatggaaa gtcaccggat ggtatatctc atcaactgat aatcagtctt
     4141 caactaaaca gattgcattt gaaggatata cagacacaga taacttctcc gattctaaat
     4201 ttatttcttc atttaaatcc tggtttgaaa gtggaaagat aaaaaacatt gctaactata
     4261 atccaaaagg agaaagagat ggaaaatacg aaagttatta caatagcaat ggtaacgtct
     4321 atgaatctct aagtttttct aatggtctta tcaacggtga gtttattaca tattatgaaa
     4381 atggggccat tgaaagtaaa cgtcatttta ataacgacaa aattaaagat ggtgaatgcc
     4441 ctcattttta tgaagatggg aaaatcaaac agcaacatag ctatctgaat agtaaactcg
     4501 atggtcctgc atatgaatat tatcctgatg gcaaactcat gcaggaaaat ttctatcagc
     4561 aaagtgtgtt gattggaaac gataccagtt actaccaatc cggaaaaata atctctatac
     4621 acaatagaaa ttcccgtggt cagtatgatg gagctaacga acgttatagt gaagagggta
     4681 agttacagtc taaatccgtc tataaagacg gaaaacaaat ttctgttcaa acctggtatg
     4741 aaaatgggca gaaagaagag gagaaacatt ttgatgagca aggccaatta aatggtctgg
     4801 ttaagcaatg gtataaaaat ggtaatcttg caaaatcaca aaactataaa catgacatac
     4861 tcgatggtaa cagtgaagag tggtacgaaa atggcattcc tgaaagtttg tacccttata
     4921 agaatggaaa aaccgacggc attgccaaat catggaataa gtatggtaaa ttaacctatt
     4981 caatcgaata caaaaatagc gtagagaatg gggcatatcg taactggagc aaaaacacag
     5041 gtaaactaac caaagaaacc ctgtacgtca atggtatcag acagggagtg gaaaaagagt
     5101 ttaacgatcg cacaggtaaa ctattaacct ccacgcaata tgttaataat aaaagacatg
     5161 gtacggagga aacttatgat caaaacggaa taaaatatat tacatgctat caaaatgatc
     5221 agaaattatc gtctttagac aatccaacac aaataaaaga taatgcaaca acaggcgatt
     5281 ccagtgctca atttgcatta gggaaatacg aattcatctg tgcaaatata gatgaaggga
     5341 ttaaatggtt aaccaaatct gcagagcaaa aaaaacactg atgcgatata ctttttagct
     5401 acagcctaca aa
//
LOCUS       DAJTXY010000195         5376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTXY010000195 DAJTXY010000000
VERSION     DAJTXY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.197"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(492..1010)
                     /locus_tag="OO566_003958"
                     /pseudo
     CDS             complement(492..1010)
                     /locus_tag="OO566_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1027..1712)
                     /locus_tag="OO566_003959"
                     /pseudo
     CDS             complement(1027..1712)
                     /locus_tag="OO566_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2402..4111)
                     /locus_tag="OO566_003960"
     CDS             complement(2402..4111)
                     /locus_tag="OO566_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6607025.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(4543..5262)
                     /locus_tag="OO566_003961"
     CDS             complement(4543..5262)
                     /locus_tag="OO566_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6607026.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1491 a         1125 c         1051 g         1709 t
ORIGIN      
        1 tgagtttgtt acgcgtaata tattttttgt aatgcttttg ttgctttatt cggattcgtt
       61 tttccatcaa caagatcaat attattctga acattaatat gatttactgc ttcaggtatt
      121 ttcataaaat attccccatt atgtgcgttt ttatctatgg ttatttagaa tatattatct
      181 aacttaatat tttcttgtgt gtattataac gaaatgaaca tattttaata tgttgtacaa
      241 tttttactct gaaatcccct gaaattaatc acacctcctt tactgaaagg aattattgca
      301 acggtattat ttgtttttgg atatatatct tgcgccttgt tatgtagtta atcctcagaa
      361 tatgatagat aaaatagaga cacagagcag agcgtgttct aacatcatga taacataaaa
      421 taattagtaa caataaatga aagaatcatg ggagctcaac aaaaagagag ctaaatagct
      481 gtgtggaata gtagatcact ttggcggtat aatggcagat ggtctgcata ctttccggta
      541 acaacatggg ccagaagccc cgctccggca taactgcgtg caatgggttt tgaaggcacc
      601 ggtgcctgca cgatatggtc gcaccggcga caggccagtt tagggcgttg tttttcgata
      661 accttaaagg cgctgctgat aagctccagc tgctctgaca catcacatcc cagtggactg
      721 agttcaccac cacaggccgg acagcattcc tcttccggcc ggatgacccg gggggcacgg
      781 ggaagtgagg ctggtaacgg tttacgggct gaagactggc gcagggaaga tggcagtacc
      841 gggtcatatt gctcacccag tgtttccgcc atttcctcct gaagtgcgct gattcgctcc
      901 tgtgcttcct gtatccgccg ttcggttttt gcgcgaagtt tttctgagct tttaccgaac
      961 tgcatacgct gcagtttcgc gaccagcgcc ttcagtcggt tgatttcgga aggcctggac
     1021 aactgaatct gcgacccaga cctgattagg tgctgtgggc gtaaagttct gattcagcag
     1081 attcggcgta accggcagat tgtggtcaga gtttgtggtc gccctgaact tgcgtttctg
     1141 cttacagtgc agcctcagct ccttacgaag acgtgccagt ctgtcacgac cgacgataat
     1201 tccgttatct gccagctccg tctggagccg ccgggttcca tatgtttcgc gagtgcggat
     1261 atgtgccacc ttaatctcca gttttagccg ctcatcactt tgttttctgt ctgagggggc
     1321 atgctgtacc cggtcgtaat aaccgctcct ggatacacca ggtacccgac acatcgcttc
     1381 aacagggaat tgttgtcgcc attgttcgac taacgcgtat ttttcagcga ctcctgggca
     1441 aaatacgctg ttgctttttt taatatatct cgctcaagtc gtgcttcatt taaagcctta
     1501 cgcagttgca taacttcaga ttccagctca gccactgtgc gggagcctgg tgtattgagc
     1561 ccttttcttg cggcggtaac ccattgtcct aaagtgcctt caggaagaga taatcgggaa
     1621 gcgccttcac tgatcgaaag ctgattttca ggaaccgttt tgacggcttc ggctttaaac
     1681 tccgtagagt aacgttggtt ttttctgctc attattggct ccttctgatg ccattctatt
     1741 tcaggaagga gtgtccgtta aactcaggct acctcagata agcttgatac aactggtctg
     1801 gtatatacag tttctttgag tgcggttacg tccacagagg taacggtaaa agtaacccct
     1861 tgtcgggacc actccgaccg cgcacagagg cattttcatc gctgacaggc cgcattgcct
     1921 cgcagggctg gaggaggatc ggtgatatcc ggacgaccgt accggcttac gccgggcttc
     1981 ggcggtttta ctcctctgcc atgtgcaaca acggagtgcc gccttacatg ccgctggcgc
     2041 ggcatatcct ggtaacattt ttgttgatac caccttccgg ccttatcctg accagtgggc
     2101 tttcctggca tcgatgatac cgatgaacgc gcatgatatt gagccgacga tattacgggc
     2161 aactggaaat actcactctc tggatgtgac ctttatccat gaagaagatt tggccacacc
     2221 atggaaacct gaacaatcat cagtttacgc ccatgtaaac ccctacggga tatttgaact
     2281 cgatatggaa acaaggttgc caatcgaggt ggttgcctga aagttggcag ttacccggtc
     2341 actacacctt gttggagtga acatatcaaa aacaggtaat tacacaattt aaattacagt
     2401 ctcataaaaa atgattaaat ctatttatta tttcaataaa cgtttttttc atcttctttg
     2461 aaaattgttc tgctatagta tccggttcca gattgttctc ttttattatt ctacaatact
     2521 ctaatattgt ttctttgtct ctatttacta cagatacaaa tacaccagga ataccatctt
     2581 tattagttgc ggacagcatt tcgaccaatc gtcttgtggt aaaactatag ttttttagaa
     2641 tattacaaaa ggcacgtatt gtttcggtat ggccattata aaatgccata aaaagtcctg
     2701 atgtaccgtt gggatttttt atccataata attcatctat atattcctgg tgagggggga
     2761 gtatttttag tatatctcca tacgccataa tggcctttgt gtgcccattt tgaagtgcca
     2821 taaacaaccc atgctttact ttcaataatt ctttaatttg ttcactacta agcattttct
     2881 tattaagcag acttccgaac atacgaatac tatctgcatg tccattctgc aatgcaaaaa
     2941 ataacccagg taaatcatgg ctgtctttcg cagctaatag atataatctc ttatcggaag
     3001 ttaacggggt tttaaagact atttccccat aggcttttat tgtgtctaca tgtccgttca
     3061 taaaagacat gcataaaccc ggcacattgt ttgcccctac cgcagcaagc aatgtaagta
     3121 ttgtttcttg actaagattg cacatattta atatcttacc atatacttga ataatgtctg
     3181 aatatccgtt ttgaagtgct atataaaatg caggagtacc atcagagctt ctcgtggcca
     3241 gaaggttcac cagtttagat tgagaaattt caccattttt gtatttttcc atcagtataa
     3301 ataatacttc tgtcagattc tttgttatcg catgatatat aactctggaa ttgatcaatt
     3361 catctggtaa aagtttcact gtgtttgtta gaggggaatt atgcctatct tcaaatatta
     3421 cctgcccggg ttgctccaga ccataaattt tccaataaaa atcatcaaaa aaatccttag
     3481 cggataattg actcagttcg aaatggctat cacaagtaaa tacagtacgt ttatgagtaa
     3541 cagttcgatt aggatcgaaa aactgaacaa cccatttttc actttcttgt acgcgcttta
     3601 ttttcaaccc taacgccatt tggtggttta atgtgcatgc tgataatgtt ctgacaaagt
     3661 ctccattttt tttcatgtta taaaaaaaat tagtaataac tgttccccac tttttatttt
     3721 ccacaaaata aatataagct ggtgattttg aaaaatcttc cgttttttcc aaacatacat
     3781 tttttgcaat agcatcaggg gtgctaagaa gctgatagtt taccttccca tttgtttcca
     3841 taaaattcat ttcccaatag gaagctaggt gacggcaaac tatttgtctt ccatatatat
     3901 cttttgccac tccatttata tttaccttca catttctctt ttctgaaaaa taaggtgctg
     3961 ttttattctt aaactgaatt agttcactgg agtttgcgac cctcataata gaacttgtta
     4021 ttgttctatt atctctgtta acagacatat ctgttgataa cgaatttttt gttgaaaata
     4081 tactagatga aaatgtttta tttaacggca tagaagacaa ccatagagat gttataatac
     4141 tattagattg cagttactaa tagtaaaagc ctccttacaa ggaggtagct tattcagaat
     4201 gctcctgtaa gatagcgagc tcgtcaacga tacagatgat ctgattatat tgtgcaaaag
     4261 gagaacagga aaataggcac tggaaaagat gaaggcctga tgggagcatt aactaatgca
     4321 aaatccgaca ggttaatatc ccgaaaagca atcaaacgac tcaaacagga gatgcacgct
     4381 gaagtggtgg acaactgttc ggatttcaga tgagtagctc agcagtttat gagccgaatg
     4441 ctctgtcacg ggcaggaatc aggctgcata cgcagtgcca aatctaatcg tagttattct
     4501 aaccagtttt caagctacaa ggtggtgtag ctggcatctt ctctactcta tcatcaaacg
     4561 ataaaatggt tcctctcgta acatttgctc ttgcctttca cttccatctc gatcgcttcg
     4621 tagttcatta cgatagctga catatttcca gtcttcaggt ctaacatctg acgccggata
     4681 ctccccggga gcgattttgc ttcttaatag attagactcg agacacatta taaattgtcg
     4741 tattttatga agtaataaag cactatattg cgagcattcc tgatctgatt ttacatatag
     4801 cgtaaactga gctcctatcc ccacacgaga ttgctgagaa actcgattca tatcagttat
     4861 cttccattta tctataggac tgtcctctga aaataataaa ccagacaaaa tttggaatgc
     4921 cagaggaact tgctccctag ctatactaat atgaaacttg tccccaacaa aatccccctt
     4981 actctgagga ttttcacgac atgcatgaat aaacacatct ccactatggt tcacaatctg
     5041 gaatccttgg tacatgacag aaaaaaattg acatatatca gagtaagttg gcacgttcga
     5101 tgctactttg aaatagtacg ctgggtattg ccgtatttgg ttgtacaata tattgaagtt
     5161 atttttcaat ctttcattcg cagaaagcat ctgcggtatt tcagacctca caacattcaa
     5221 agaatctaaa tttagtttta gacagggctt ttttatgggc atagaaaacg tcctctataa
     5281 aatagatata ttatcttata ttgattgttt atcatctttt ataaaacgat acattatatg
     5341 taatgtatgt aagcgtaagg gtcttctccg atcatg
//
LOCUS       DAJTXY010000196         5337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTXY010000196 DAJTXY010000000
VERSION     DAJTXY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.198"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(36..773)
                     /gene="bamD"
                     /locus_tag="OO566_003962"
     CDS             complement(36..773)
                     /gene="bamD"
                     /locus_tag="OO566_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6607027.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            908..1888
                     /gene="rluD"
                     /locus_tag="OO566_003963"
     CDS             908..1888
                     /gene="rluD"
                     /locus_tag="OO566_003963"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6607028.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1885..2616
                     /gene="pgeF"
                     /locus_tag="OO566_003964"
     CDS             1885..2616
                     /gene="pgeF"
                     /locus_tag="OO566_003964"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6607029.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2746..5319
                     /gene="clpB"
                     /locus_tag="OO566_003965"
     CDS             2746..5319
                     /gene="clpB"
                     /locus_tag="OO566_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6607030.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1290 c         1454 g         1284 t
ORIGIN      
        1 gctcaagggc tgccgttttg tgtttcaggt ttctgttatg tattgctgct gtttgcggcg
       61 atgattttcg ctactttttc agcttgcgca ttcatctgca tctgacggta tgcattttcc
      121 atcagcggca gcgcatcacg cgtagcctgg gtatccgggt agtcgcgcaa catgccttct
      181 acgcggttaa cgacggcaac ccatgcgcca cgttctgtat agtactcggc cacggagtat
      241 tcatatttcg ccagacgatc tttcaggaat accagacgtt tggtggcatc ggtagtgtac
      301 tgactgttcg gatagccgcg caccagtttg gaaaagtcac taaacgcagc tcgtgcatgt
      361 tgaggatcgc gatcgctacg gtcaacgcca aagaaccctt gcagcgcact gtcatccagc
      421 gccatattgg tcaggccacg catgtacatg acataatcga tattcggatg ggtcgggtta
      481 aggcgaataa aacgatcgat ggcagcctgc gctaacggca aatcggcgtt tttatagtag
      541 gcgtagatga gatccagctg cacctgctgc gaatacggac caaacggata gcgattatct
      601 aacgcttcca gttgcgttat tgcctgtctc cagttaccgt cctgcagctt ttgttgtgca
      661 gtcgcgtaaa tttcatttgg cggattatca ggtacttctt cctttgaccc cgagcaaccc
      721 gccaaaaaca ggcttagtgt ggcggctgcc accagatatt tcatgcgcgt catgacgttt
      781 tgactttcct caaaatgtaa tacgggagat tctctgttcc tgctcccggt taagaccagc
      841 tacaatagca cactatatta aacggcaaag ccgtaaaacc ccaacgataa acgaagaagc
      901 agtatatatg gcacaacgag tacagctcac tgcaacggtg tccgaaaacc aactcggtca
      961 acgcttagat caggctttgg ccgaaatgtt cccggattat tcacgttcgc gaataaaaga
     1021 atggatcctc gaccagcgcg tgctggtaaa cggcaaagtt tgtgataagc cgaaagaaaa
     1081 agtattgggt ggcgagcagg ttgccatcaa ctctgagatt gaagaagaag cgcgttttga
     1141 accgcaggat atccctctgg atatcgtcta tgaagatgaa gatatcatcg tcattaataa
     1201 accgcgcgac ctagtggtac atcctggcgc gggtaacccg gatggcacgg tactgaatgc
     1261 gttgcttcat tactatccgc ccattgccga tgtaccacgt gcgggcatcg tccatcgtct
     1321 ggataaagac accactggcc tgatggttgt ggcaaaaacc gttccggctc agacgcgttt
     1381 agtcgaatct ttgcaacggc gtgaaattac tcgtgagtat gaagcggtgg cgattggtca
     1441 tatgaccgct ggcggtacag tagatgaacc catcagccgc cacccgacca aacgtactca
     1501 catggcggtg catccgatgg gcaaaccggc ggtgactcac tatcgcatca tggaacactt
     1561 ccgtgtgcac acgcgtctgc ggttgcgtct ggaaactgga cgtacgcacc agatccgcgt
     1621 gcatatggcc catatcactc atccgctggt gggcgatccg gtttatggtg gccgtccgcg
     1681 tccgccaaaa ggtgcttcgg aagcatttat ctccacgctg cgtaagtttg accgccaggc
     1741 gcttcatgca accatgctgc gtctttatca cccgatctcc ggcatcgaaa tggaatggca
     1801 tgcgcctatt ccacaagata tggtggagct gattgaggtg atgcgcgccg atttcgaaga
     1861 gcataaggat gaagtggact ggttatgagt aagctgattg tcccgcagtg gccgctgcca
     1921 aaaggtgttg cggcctgtag ctccactcgt atcggcggcg tgagcttgcc cccgtatgac
     1981 tcactcaacc tcggtgccca ttgtggcgat aacccggatc acgttgagga gaatcgcaag
     2041 cgactttttg ctgcgggcaa tttgccttct aaaccggtct ggcttgagca ggtacacggc
     2101 aaagatgtgc ttaagcttac tggcgaacct tatgcctcaa aacgagcaga tgcctcttat
     2161 agcaatacgc ctggcacggt ttgcgcagtg atgactgccg actgcctccc tgtgctgttt
     2221 tgcaatcgag cgggaacgga agtcgccgcc gttcatgctg gctggcgtgg actgtgcgca
     2281 ggcgtgctgg aagagacggt ttcctgtttt gctgataaac ctgaaaacat tctcgcctgg
     2341 ttagggccgg caattggtcc acgcgcgttc gaagtggggg cggaggttcg cgaggcgttt
     2401 atggcagtag acgctaaagc aagtgcagct ttcattcagc atggtgataa gtatctggcg
     2461 gatatttatc agcttgcccg gcagcgtctg gcgaacgtgg gtgttgagca aattttcggc
     2521 ggcgaccgtt gtacatatac ggaaaatgag actttcttct cttatcgtcg cgacaagacc
     2581 accggtcgta tggcaagttt catttggctg atataaccta aagaatcaag acgatccggt
     2641 acgcgtgatt ttcttttcac attaatctgg tcaataacct tgaataattg agggatgacc
     2701 tcatttaatc tccagtagca actttgatcc gttatgggag gagttatgcg tctggatcgt
     2761 cttactaata aattccagct tgctcttgcc gatgcccaat cacttgcact cgggcacgac
     2821 aaccaattca tcgaaccact tcatttaatg agcgccctgc tgaatcagga agggggttcg
     2881 gttagtcctt tattaacatc cgctggcata aatgctggcc agttgcgcac agatatcaat
     2941 caggcattaa atcgtttacc gcaggttgaa ggtactggtg gtgatgtcca gccatcacag
     3001 gatctggtgc gcgttcttaa tctttgcgac aagctggcgc aaaaacgtgg tgataacttt
     3061 atctcgtcag aactgttcgt tctggcggca cttgagtctc gcggcacgct ggccgacatc
     3121 ctgaaagcag caggggcgac caccgccaac attactcaag cgattgaaca aatgcgtgga
     3181 ggtgaaagcg tgaacgatca aggtgctgaa gaccaacgtc aggctttgaa aaaatatacc
     3241 atcgacctta ccgaacgagc cgaacagggc aaactcgatc cggtgattgg tcgtgatgaa
     3301 gaaattcgcc gtaccattca ggtgctgcaa cgtcgtacta aaaataaccc ggtactgatt
     3361 ggtgaacccg gcgtcggtaa aactgccatc gttgaaggtc tggcgcagcg tattatcaac
     3421 ggcgaagtgc cggaaggatt gaaaggccgc cgcgtactgg cgctggatat gggcgcgctg
     3481 gtggctgggg cgaaatatcg cggtgagttt gaagaacgtt taaaaggcgt gcttaacgat
     3541 cttgccaaac aggaaggcaa cgtcatcctg tttatcgacg aattacatac catggtcggc
     3601 gcgggtaaag ccgatggcgc aatggacgcc ggaaacatgc tgaaaccggc gctggcgcgt
     3661 ggtgaattgc actgcgtagg tgccacgacg cttgacgaat atcgccagta catcgaaaaa
     3721 gatgctgcgc tggaacgtcg tttccagaaa gtgtttgttg ccgagccttc tgttgaagat
     3781 accattgcga ttctgcgtgg cctgaaagaa cgttacgaat tgcaccacca tgtgcaaatt
     3841 actgacccgg caattgttgc agcagcgacg ttgtctcatc gctacattgc tgaccgtcag
     3901 ctgccggata aagccatcga cctgatcgat gaagcagcat ccagcattcg tatgcagatt
     3961 gactcaaaac cagaagaact cgaccgactc gatcgtcgta tcatccagct caaactggaa
     4021 caacaggcgt taatgaaaga gtctgatgaa gccagtaaaa aacgcctgga tatgctcaac
     4081 gaagaactga gcgacaaaga acgtcagtac tccgagttag aagaagagtg gaaagcagag
     4141 aaggcatcgc tttctggtac gcagaccatt aaagcggaac tggaacaggc gaaaatcgct
     4201 attgaacagg ctcgccgtgt gggggacctg gcgcggatgt ctgaactgca atacggcaaa
     4261 atcccggaac tggaaaagca actggaagcc gcaacgcagc tcgaaggcaa aactatgcgt
     4321 ctgttgcgta ataaagtgac cgatgccgaa attgctgaag tgctggcgcg ttggacgggg
     4381 attccggttt ctcgcatgat ggaaagcgag cgcgaaaaac tgctgcgtat ggagcaagaa
     4441 ctgcaccatc gcgtcattgg tcagaacgaa gcggttgatg cggtatctaa cgctattcgc
     4501 cgtagccgtg cggggttggc ggatccaaat cgcccgattg gttcattcct gttcctcggc
     4561 ccaacgggtg tggggaaaac tgagctttgt aaggcgctgg cgaactttat gtttgatagc
     4621 gacgaggcga tggtccgtat cgatatgtcc gaatttatgg agaaacactc ggtgtctcgt
     4681 ctggttggtg cgcctccggg atatgtcggt tatgaagaag gtggctacct gaccgaagct
     4741 gtgcgtcgtc gtccgtattc cgtcatcctg ctggatgaag tggaaaaagc gcatccggat
     4801 gtcttcaata ttctgttgca ggtactggat gatgggcgtc tgactgacgg gcaggggaga
     4861 acggtcgact ttcgtaatac ggttgtcatt atgacctcta acctcggttc cgatctgatt
     4921 caggaacgct tcggtgaact ggattatgcg cacatgaaag agctggtgct cggtgtggtt
     4981 agccataact tccgtccgga attcattaac cgtattgatg aagtggtggt cttccatccg
     5041 ctgggtgaac agcacattgc ctcgattgcg cagattcagt tgaaacgtct gtacaaacgt
     5101 ctggaagaac gtggttatga aatccacatt tctgacgagg cgctgaaact gctgagcgag
     5161 aacggttacg atccagtcta tggtgcacgt cctttgaaac gtgctatcca gcagcagatc
     5221 gaaaacccgc tggcacagca aatactgtct ggtgaattgg ttccgggtaa agtgattcgc
     5281 ctggaaatta atgaagaccg gattgtcgct gtccagtaat gataaaacga gcccttc
//
LOCUS       DAJTXY010000197         5320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTXY010000197 DAJTXY010000000
VERSION     DAJTXY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.199"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..609
                     /locus_tag="OO566_003966"
                     /pseudo
     CDS             1..609
                     /locus_tag="OO566_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            673..1599
                     /gene="glsB"
                     /locus_tag="OO566_003967"
     CDS             673..1599
                     /gene="glsB"
                     /locus_tag="OO566_003967"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6607031.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1566..1958
                     /locus_tag="OO566_003968"
     CDS             1566..1958
                     /locus_tag="OO566_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6607032.1"
                     /translation="MDETVRAFGLLRQSLDPLFARLSRSKFRSRFRLGMKERQYCLEK
                     GAPVIEQHAADFVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIP
                     QGVSLSEEQQRYIVAVIYHWLVVQMNQP"
     gene            2097..3515
                     /locus_tag="OO566_003969"
     CDS             2097..3515
                     /locus_tag="OO566_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6607033.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3742..5193
                     /gene="uxaB"
                     /locus_tag="OO566_003970"
     CDS             3742..5193
                     /gene="uxaB"
                     /locus_tag="OO566_003970"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6607034.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1228 a         1238 c         1433 g         1421 t
ORIGIN      
        1 ttcccatcat tcttgcaggt aacggctact tacttaaaca tgcgccgaat gtgatgggct
       61 gtgctcagct cattgcccag gtgtttaaag atgcgggaat cccgcaaggc gtatatggct
      121 ggctgaatgc cgacaacgac ggtgtcagtc agatgattaa agactcgcgc attgctgctg
      181 tcacggtgac cggaagtgtt cgtgctggag cggctattgg cgcacaggct ggagcggcac
      241 tgaaaaaatg cgtactacta tccgccaacg gttctggcga atgttacccc agaaatgacc
      301 gcgtttcggg aagaaatgtt tggccccgtt gctgcaatca ccattgcgaa agatgcagaa
      361 catgcgctgg aactggctaa tgatagtgag ttcggccttt cagcgaccat ttttaccacc
      421 gacgaaacac aggccagaca gatggcggca cgtctggaat gcggtggggt gtttatcaat
      481 ggttattgtg ccagcgacgc gtgagtggcc tttggtggcg tgaaaaagag tggctttggt
      541 cgtgagcttt cccatttcgg cttacacgaa ttctgtaata tccagacagt gtggaaagac
      601 cggatctgac gcgacatatt cagctctgat atactcgcag gtcttttcag acctgcggtc
      661 caggagtaga aagtggcagt cgccatggat aatgcaattt tagaaaacat cttgcggcaa
      721 gtgcggccgc tcattggtca gggtaaagtc gcggattata ttccggcgct ggctacagta
      781 gacggttccc gattggggat tgctatctgt accgttgacg gacagctttt tcaggccgga
      841 gacgcgcaag aacgtttttc cattcagtct atttccaaag tgctgagtct cgttgtcgcc
      901 atgcgtcatt actccgaaga ggaaatctgg caacgcgtcg gcaaagatcc gtctggatca
      961 ccgttcaatt ctttagtgca actggaaatg gaacagggta ttccgcgtaa tccgttcatt
     1021 aatgccggtg cgctggtggt ctgcgatatg ttgcaagggc gactaagcgc accacggcaa
     1081 cgtatgctgg aagtcgtgcg cggcttaagc ggtgtgtctg atatttcata cgatacggtg
     1141 gtagcgcgtt ccgaatttga acattccgcg cgaaatgcgg ctatcgcttg gctgatgaag
     1201 tcgtttggca atttccatca tgacgtgaca accgttctgc aaaactactt tcattactgc
     1261 gctctgaaaa tgagctgtgt agagctggcc cggacgtttg tctttctggc taatcagggg
     1321 aaagctattc atattgatga acctgtggtg acgccaatgc aggcgcggca aattaacgcg
     1381 ctgatggcga ccagtggtat gtaccagaac gcgggggagt ttgcctggcg ggtgggacta
     1441 ccggcgaaat ctggcgttgg tggcggtatt gtggcgattg ttccgcatga aatggccatc
     1501 gcagtctgga gtccggaact ggatgatgca ggtaactcgc ttgcgggtat cgccgtcctt
     1561 gaacaattga cgaaacagtt agggcgttcg gtttattaag gcagtctctc gatccactct
     1621 tcgcgcgttt atcccgttca aaatttcgct ctcgctttcg tctgggcatg aaagagcgtc
     1681 agtattgcct ggagaaaggc gcgccagtca tcgaacaaca tgcggcggat tttgtcgcta
     1741 aacgtcttgc tccggcatta ccggctaatg acggtaagca accccccatg cgcgggcatc
     1801 cggtgtttat cgctcagcac gccaccgcga cctgctgtcg cggttgtctg gctaagtggc
     1861 ataacattcc gcagggtgtt tcgttaagcg aagaacaaca acgttatatc gtggcggtaa
     1921 tctatcactg gctggttgta cagatgaatc agccatgaga attgcggaaa acgataaaat
     1981 agcattccca gggtaccttt atttgccgcc gctgttagcg aacatgctat cctttatgag
     2041 aatttactta tcacgttgag ataattgcca cccgcaattg tatggacaga taattaatgc
     2101 atgtacaccc catttcaact ttccgtttgt tccaggaagg tcatctgcta cgtaatagca
     2161 tcgctatttt tgcgctaacc acgctgttct attttattgg tgcagagtta cggctggttc
     2221 acgaactttc tcttttctgg ccgctgaatg gcgtaatggc gggggtgttt gcccgctatg
     2281 tctggcttaa tcgactgcat tactatgcga tcagttatgt ggcgatgctg gtttatgatg
     2341 ccataaccac cgaatgggga ctggtttcac tggttatcaa tttctccaat atgatgttta
     2401 ttgttaccgt cgccttactg gtcgtgcggg ataagcgtct ggggaaaaat aagtatgagc
     2461 cagtaagtgc cttacggcta tttaattact gtctgattgc cgcattatta tgcgctattg
     2521 tcggggcgat tggttcggtc agtattgata gtctggattt ctggcctttg cttgcggact
     2581 ggttcagtga gcaattctca acgggcgtgt tgatcgtgcc ttgtatgctg acgttggcta
     2641 ttcctggagt gctgccgcgc tttaaagcag aacagatgat gcccgccatc gcgcttattg
     2701 tgtcggttat tgcctcggta gtcattggcg gagcggggag tctggcgttc ccgctgcctg
     2761 cattaatctg gtgtgcagtg cgctatacgc cgcaggtaac atgtctgttg acctttgtca
     2821 ccggtgcggt ggaaatcgta ctggtggcaa attcggtgat tgatatctcg gtcggttcgc
     2881 cgttctccat tccggaaatg ttctccgcac gtctcggtat tgccacgatg gcgatatgcc
     2941 caattatggt ttcttttagc gtggcagcga tcaatttgct aatgaagcaa gttgcgctgc
     3001 gagccgactt tgattttctg actcaggttt actcacggtc cggtctttat gaggcgctga
     3061 aaagtccatc gctgaaacag acacaacatc tgacggtcat gctgcttgat atcgactatt
     3121 tcaaaagcat taacgataac tatggacatg aatgtggcga taaagtgtta agcgtgtttg
     3181 cccggcatat tcagaagatt gtcggtgata aggggctggt ggcgcgcatg ggcggcgaag
     3241 aatttgctgt tgcagtgccg tcggtgaatc ctgtcgatgg tctgctaatg gcggaaaaaa
     3301 tccgtaaagg cgttgaactg caaccattca cctggcaaca aaaaacgctt tatctcacgg
     3361 taagtattgg cgtcggtagt ggtcgcgcat cgtaccgaac gctgaccgat gattttaata
     3421 aattgatggt cgaagccgat acgtgtctgt atcgctcgaa gaaagatggg cgcaaccgta
     3481 ccagcactat gcgttacggt gaggaagttg tctgacatct ttattcattc cgcgcgattt
     3541 gaatgcaatg tgttctacca ctttatgatc caggaaaagg cgtatttcct cgattaaacc
     3601 atgatccgcg ccacactttt ctcataaaac tgctcattaa tcatcaggag ttgatcaaaa
     3661 caggggttcg gcgtattaaa gtttgctaac ctgtcgcacc aattcaaaca ggttgtatga
     3721 ctaatcagaa gggaacccat tgtgaaaaca ctaaatcgtc gcgattttcc cggtgcacag
     3781 tatccagaac gtatcattca gtttggtgaa ggtaacttcc tgcgcgcctt tgttgactgg
     3841 caaatcgatc tcctgaatga gcacaccgat ctgaattctg gcgtggtcgt tgttcgtccg
     3901 attgaaactt cattcccgcc gtcactgagc acgcaggatg gtctgtacac caccattatc
     3961 cgtggcctga atgagaaggg ggaagcggtc agcgacgcgc gtctgattcg ctcggttaat
     4021 cgtgaaatca gcgtctacag tgaatacgat gaattcctga aactggcgca caacccggaa
     4081 atgcgctttg ttttctctaa caccaccgaa gcgggtatca gctaccatgc gggcgataaa
     4141 ttcgatgacg cgccagcggt aagctatccg gcaaaactga cccgtctgct gttcgaacgc
     4201 tttagccatt tcaacggtgc gctggataaa ggttggatca tcattccgtg tgagttgatt
     4261 gactacaatg gcgacgcgtt gcgtgaactg gtgctgcgct atgcgcaaga gtgggcactg
     4321 ccagaagcat ttattcaatg gctggatcag gctaacagct tctgttctac gctggtggac
     4381 cgtatcgtta ccggttatcc gcgcgatgaa gtggctaaac tggaagaaga actgggttat
     4441 cacgatggtt tcctcgacac cgctgaacat ttttacctgt ttgtgattca ggggccgaaa
     4501 tccttagcga ctgagctgcg cctggacaaa tatccgctca acgtgctgat tgttgacgat
     4561 attaaaccgt ataaagaacg caaagtggca atcctcaacg gtgcgcacac cgcgctggtg
     4621 ccggtggcgt tccaggcagg gctggatacc gtaggtgaag cgatgaacga tgctgaaatt
     4681 tgcgcattcg ttgaaaaagc catctacgaa gaaattattc cggtactgga tttgcctcgt
     4741 gatgaactgg aatctttcgc cagtgcagtc accgggcgtt tccgtaaccc gtacattaag
     4801 catcagctgc tgtctatcgc gctcaacggt atgaccaagt tccgcacccg catcctgccg
     4861 cagctgctgg cagggcagaa ggcaaacggc acacttccgg cgcgccttac tttcgcatta
     4921 gcggcattga ttgcgttcta tcgtggtgag cgtaacggag aaacatatcc ggtacaggac
     4981 gatgcacact ggctggaacg ttatcagcaa ctctggagcc agcatcgtga ccgtgttatc
     5041 ggtacccagg aactggtagc gattgtgttg gcagaaaaag accactggga gcaggacctg
     5101 acgcaagtgc ctggtctggt cgagcaggtt gctaatgacc tggatgcgat tcttgaaaaa
     5161 gggatgcgcg aagctgtacg tccgttgtgc taataacacc gttacttctg aacccggttt
     5221 cgaccgggtt tcttatttca ctatattagt tacaacatcc tatatgcctt tatcattaac
     5281 aggtgtcaca tgcagcgcct gttaatgttg taagcttgac
//
LOCUS       DAJTXY010000198         5289 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTXY010000198 DAJTXY010000000
VERSION     DAJTXY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.200"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..588
                     /locus_tag="OO566_003971"
     CDS             <1..588
                     /locus_tag="OO566_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6607035.1"
                     /translation="TTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIH
                     MYKIATLGSGNPRNFTCNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYC
                     SSEQKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVN
                     KSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            922..3201
                     /gene="ydeP"
                     /locus_tag="OO566_003972"
     CDS             922..3201
                     /gene="ydeP"
                     /locus_tag="OO566_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6607036.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3449..3646
                     /gene="safA"
                     /locus_tag="OO566_003973"
     CDS             3449..3646
                     /gene="safA"
                     /locus_tag="OO566_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6607037.1"
                     /translation="MHATTVKNKIMQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3720..4481
                     /gene="ydeO"
                     /locus_tag="OO566_003974"
     CDS             3720..4481
                     /gene="ydeO"
                     /locus_tag="OO566_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6607038.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4883..>5289
                     /locus_tag="OO566_003975"
     CDS             4883..>5289
                     /locus_tag="OO566_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR6607039.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSR"
BASE COUNT         1529 a         1126 c         1170 g         1464 t
ORIGIN      
        1 acgacaaata ccaacgtatt ggatattggt gataagacac cgatgccatt accgttaaaa
       61 ctctatatta ccccagttgg ggcggccggt ggagttgtca taaaagcggg ggaagtcatt
      121 gcccgtattc atatgtataa aattgcaaca ttaggaagcg gaaatccgcg taattttacg
      181 tgtaatatta tttcaaataa cagtgttgtt atgccaacag gcggctgcac cgttgatagt
      241 cgtaatgtca ccgtggatct cccggacttt cctggaagcg cagaaatacc ccttggcgta
      301 tattgctcaa gcgaacaaaa gctctcattt tatctttcgg gtgccaccac tgacagtagc
      361 cgacaagtgt ttgctaacac agcacctgat gcaacaaaag catcgggagt gggtgtcact
      421 ctaatgcgta atggaaaaat actggctacg ggagaaaatg tctccctggg gacagtaaat
      481 aaaagcaaag tacctctggg tttaagcgca acctatggac aaacaggaaa caaggtttca
      541 gccggaaccg tccagtcagt cattggtgtc acttttatct atgaataatt gtgattttga
      601 ccttagtcta tgcgctccat tcacgaaaat tgcgcggata attattttgt gaaggctatt
      661 atcctacacc tgtaagaaaa tccgcgcaca attgaagcca gagtcttatt ttttatattg
      721 aaaaatatgt taatttaaac actatgagag atgatgctta acttatcgta tttaatctat
      781 tgtttaacga taatagatta aaaatagaaa gagagtagtc ccctactctt atatatccat
      841 gttggcgata atccctttgt atgtactgtg catcatcgct attacaaatc ctaataattc
      901 atttccacac aggataagta gatgaagaaa aaaattgaat cctaccaggg tgctgcaggt
      961 ggttggggtg ctgttaaatc cgtagcgaat gcagtacgta agcagatgga tatacgccat
     1021 gatgttattg ccatgtttga catgaataag ccagagggct ttgactgtcc gggttgtgca
     1081 tggccagatc ctaagcacag tgcgtcattc gacatttgtg aaaacggcgc aaaagcaatc
     1141 gcctgggaag tcacggataa gcaggtaaat gcctctttct ttgctgagaa tacggttcaa
     1201 tcattactta cctggggaga tcacgagctt gaggctgcgg ggcgtctcac tcagcctttg
     1261 aaatatgatg ccgtcagcga ctgttacaag ccattaagct ggcaacaagc tttcgacgaa
     1321 attggcgcac gccttcaaag ctatagtgat cccaatcagg ttgaattcta tacttcgggc
     1381 cgcacttcca atgaagctgc ctttctttat cagctttttg cccgtgaata cgggagcaat
     1441 aactttcccg actgctccaa catgtgccat gaaccgacaa gcgtgggttt ggcagcgagt
     1501 atcggtgtag gtaaagggac cgtgttgctg gaagactttg agaagtgcga tttagtcatt
     1561 tgcattgggc ataaccctgg tacaaaccac cctcgcatgc tgacttcgtt gcgcgcttta
     1621 gtgaaacggg gagcgaaaat gatcgccatc aatcctctac aggaacgtgg cctggagcga
     1681 tttaccgcac cgcaaaaccc gtttgaaatg ctgacgaact ctgagactca gttggccagt
     1741 gcctactaca acgtgcgcat tggtggcgat atggcgttgc tcaaggggat gatgcgcctg
     1801 ttaattgagc gcgatgatgc tgcaagtgcc gcaggtcggc cctcattgct tgatgacgaa
     1861 tttattcaaa cgcataccgt cggctttgac gagctacgcc gtgacgttct caattccgag
     1921 tggaaagata tcgaacgtat ttctggacta agtcagacac aaatcgccga actggctgac
     1981 gcatatgccg ctgccgaacg caccattatc tgttacggaa tggggatcac tcagcacgaa
     2041 catggtaccc agaacgtgca gcaactggtc aatttgctgt tgatgaaagg taacattggc
     2101 aagcctggtg cgggtatctg cccactacgt ggtcactcta atgtacaggg cgaccgaacc
     2161 gtcggtatca ccgagaaacc gtctgcagag tttctggctc gcctgggtga gcgctatggc
     2221 ttccccccac ctcatgcacc tggacatgct gcaattgcca gcatgcaagc aatatgtacg
     2281 gggcaggctc gagcattgat ctgcatgggg ggcaactttg cactggcaat gccagatcgg
     2341 gaagcgagcg ctgtaccgtt aacgcaatta gatttggcgg tacacgtagc cactaagctt
     2401 aaccgctctc atctgctgac cgcacggcat agctatattc tgccggttct ggggcgtagc
     2461 gagattgata tgcaaaaaag cggtgcgcag gcggtaaccg ttgaggattc aatgtcgatg
     2521 attcatgcct cgcgtggcgt gttaaaaccc gccggtgtaa tgctgaaatc agagtgtgca
     2581 gtggtcgcgg gaatcgcgca ggcagcacta ccccagagcg tggtagcctg ggagtatctg
     2641 gtggaagatt atgatcgcat tcgcaatgac attgaagctg tgctgccaga gttcgccgac
     2701 tataaccagc gcatccgtca tcccggtggt tttcacctga taaatgcagc tgctgaaagg
     2761 cgctggatga cgccgtcagg taaggctaat ttcattacca gcaaagggct gttagaagat
     2821 ccctcttcag cgtttaacag taagctggtc atggcgacag tacgcagcca cgatcagtac
     2881 aacacgacga tttatggtat ggatgatcgc tatcgagggg tattcggtca acgagatgtg
     2941 gtctttatga gtgctaaaca agctaaaatt tgccgtgtaa aaaacggcga aagagttaat
     3001 cttattgcgc ttacgccaga cggtaagcgc agctcacgcc gcatggatag attaaaagtg
     3061 gtcatttacc ctatggctga ccgctcactg gtgacctatt ttccagaatc gaatcacatg
     3121 ctaacacttg ataaccacga tccattaagt ggcattcctg gctataaaag tattccggtt
     3181 gaattagaac catcaaatta atgcctcttc tcatttcttc tgctgtcatc cgcacagcag
     3241 aagaattcct cattgactat tattgcgcaa tttactcaca tggattaaat taaactacat
     3301 actataagat ataaacttct gcctacagct gtaagaaact ccgctcagta ctgaagcacc
     3361 agtcctattt cttcttttct ccagcctgtt atattaagca tactgattaa cgacttttaa
     3421 cgttatccgc taaataaaca tatttgaaat gcatgcgacc acagtgaaaa acaaaatcat
     3481 gcaaagagat aactataaag aaatcatgtc tgcaattgtg gttgtcttat tactgacact
     3541 tacgttgata gccatttttt cggcaattga tcagctgagt atttcagaaa tgggtcgcat
     3601 tgcaagagat cttacacatt tcattatcaa tagtttgcaa gactgaaaat aaacagcaaa
     3661 ttataaatgt gaaatgttta aaaggtatcg ataaaacttt attgttttaa ggagataaaa
     3721 tgtcgctcgt ttgttctgtt atatttattc atcatgcttt caacgctaac attttagata
     3781 aagattacgc cttctctgac ggcgagatcc tgatggtaga taacgctgtt cgtacacatt
     3841 ttgaacctta tgagcggcat tttaaagaaa tcggatttaa tgaaaatacc attaaaaaat
     3901 atctacaatg cactaacatc cagacagtga cggtgcctgt tcctgcgaag tttttacgtg
     3961 cttcaaatgt accgactgga ttgcttaatg aaatgattgc ttatctcaac tcggaagaac
     4021 gcaatcatca taatttttca gaacttttgc ttttttcttg cctgtctatt tttgccgcat
     4081 gcaaaggttt cattacacta ttaactaacg gtgtgctatc cgtttccggg aaagtgagaa
     4141 atattgtcaa catgaagctg gcgcacccat ggaagctgaa agatatttgt gactgcctgt
     4201 acatcagtga aagcctgttg aagaaaaaac taaagcaaga gcaaacgaca ttctcacaga
     4261 ttctattaga tgccagaatg cagcacgcaa aaaatttgat acgcgtagaa ggttcagtca
     4321 ataaaattgc cgaacaatgt ggttatgcca gtacatctta ttttatttat gcgttccgca
     4381 aacatttcgg caacagtccg aagagagttt ctaaggagta ccgttgtcaa aggcacacgg
     4441 gtatgaatac gggcaacacg atgagtgctt tagctatttg attatttgct aacgagtagt
     4501 caaccacgca cgctacgtaa gaattatatg gggcagccat tccctgcccc gcgttgtttt
     4561 taggcgatat atttattgaa ataaataagt gacatccatc acatatttat gcacttgcat
     4621 aacctgttgc atgattattt ataatctcaa ttcggcattt tgtcagtaaa atgcaataat
     4681 ttattaaata tcaataaatt agttatttat cggcgagaaa ttacttaata gaacagaaag
     4741 taatgtcaac gctttatgga ctgttatttc ccttttttta gctaaatctg ctatctcttt
     4801 atgtgactaa cttcacttac atccacttat ttctcttcgt aaaattactt cggaattaag
     4861 tacaataaga agaggaacat ttatgaagtc tgcattaaag aaaagtgtcg taagtacctc
     4921 gatatctttg atactggcat ctggtatggc tgcatttgct gctcatgcgg cagatgatgt
     4981 aaagctgaaa gcaaccaaaa caaacgttgc tttctcagac tttacgccga ctgaatacag
     5041 taccaaagga aagccaaata ttatcgtact aaccatggat gatcttggtt atggacaact
     5101 tccttttgat aagggatctt ttgacccaaa aacaatggaa aatcgtgaag ttgtcgatac
     5161 ctacaaaata gggatagata aagccattga agctgcacaa aaatcaacgc cgacgctcct
     5221 ttcattaatg gatgaaggcg tacgttttac taacggctat gtggcacacg gtgtttccgg
     5281 cccctcccg
//
LOCUS       DAJTXY010000199         5106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTXY010000199 DAJTXY010000000
VERSION     DAJTXY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.201"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            103..1011
                     /locus_tag="OO566_003976"
     CDS             103..1011
                     /locus_tag="OO566_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6607040.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFY
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAADKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPETMIPGSTVPFISTK"
     gene            complement(1057..3048)
                     /gene="tkt"
                     /locus_tag="OO566_003977"
     CDS             complement(1057..3048)
                     /gene="tkt"
                     /locus_tag="OO566_003977"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6607041.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3326..4084
                     /gene="loiP"
                     /locus_tag="OO566_003978"
                     /gene_synonym="yggG"
     CDS             3326..4084
                     /gene="loiP"
                     /locus_tag="OO566_003978"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6607042.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4290..>5106)
                     /gene="speB"
                     /locus_tag="OO566_003979"
     CDS             complement(4290..>5106)
                     /gene="speB"
                     /locus_tag="OO566_003979"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6607043.1"
                     /translation="VITGVPFDMATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDM
                     RERLNVVDCGDLVYAFGDAREMSEKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHA
                     KHFGKMALVHFDAHTDTYANGCEFDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNG
                     FTVLDACQVNDRSVDDVIAQVKQIVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSD
                     RAIKLVRGLKDLNIVGMDVVEVAPAYDQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT         1214 a         1381 c         1359 g         1152 t
ORIGIN      
        1 tttttcgcta taccccttta tagattctga attctcacct atcggctgtt ttctcctcat
       61 ctcatgtgac atattcccat actttactca aggaagattg tcatgaaagc acctgtttca
      121 ttaacctcat ttcgcccaca aaaatctctt gctatcgcca ttggcgtgct tgccgtggtt
      181 gtgctgccat ttctctcgta ttacaccgtt aatgaaggtg aacgcggcat tttgctgcgc
      241 tacggaaaaa tcgtaaaagt tgccgagccg gggctgggtt ttaagatccc ttttatggaa
      301 agcgtggaga aaatttccac gcgtaatcag gctgtggtct atcagggatt acaggcctac
      361 agccgcgacc agcaaccggc acaaatgaca gtatcagtca gcttctacat taaaccttca
      421 gaagcaggcg cggtttatac cacctacaac actattgaag cattaaaaga tcgtctaatt
      481 gtccgtcagt tgccaactca gctggagaac attttcggcc aatataccgc catcagcgcc
      541 gtacaggatc gtacaaaatt ggttcaggat ctgcaaaatg ccatgcgcaa agccgttgtt
      601 gggccagtgg taattgacgg ggtccagatt gaaaatattg atttttctga cgcttatgaa
      661 aagtcgattg aaaaccgaat gaaagcagaa gtcgccattg ccacgcgtaa gcaaaatctg
      721 gaaacagaga aaatccaggc gcaaatagcg gtaactcagg cccaggcaga agcagacagt
      781 aaactggcgg cagacaaagc agaagcggaa actattcgcg taagaggcgc ggcagaggct
      841 gaaactattc gtttaaaaag tgcggctgaa gcagaagcaa tccgcctgcg cggtgaagct
      901 ctgcgggata atcccggact tgtagccctc accactgccg aacgttggga cggtaaactg
      961 cccgaaacca tgatccccgg cagcacagtt ccgtttatct ctactaagta aaaaaagggc
     1021 cgctttcgcg gcccttctca caaaacgtct tactgattac agcagcgctt ttgctttcgc
     1081 aacaacgtta tcaacagtga agccgaactc ttcaaacagc agctctgccg gagcagattc
     1141 accgaaggtg gtcatgccga cgatagcgcc gttcaggcca acatacttgt accagtagtc
     1201 agcaataccc gcttctaccg caacgcgtgc agtaaccgct ttcggcagta cggattcacg
     1261 gtaagccgca tcctgcttgt caaatgcgtc ggtagacggc atggacacca cgcgcgcttt
     1321 cacgccttcg gcagtcagtt tttcgtaggc agcaaccgcc agttcaactt cagaaccggt
     1381 ggcgatgaag atcagttccg gctgaccagc acagtctttc agcacataac caccgcgcgc
     1441 gatgtttgcc agctgctctt cagtacgttc ctgctgcgcc aggttctgac gggagaggat
     1501 aagcgcagtc gggccgtcct gacgctcaac gccgtatttc cacgcgaccg cggattcaac
     1561 ctggtcacac ggacgccatg tagacatgtt cggggtcacg cgcagagaag cgacctgctc
     1621 taccggctgg tgagtcgggc catcttcgcc cagaccgatg gagtcgtggg tgtaaaccat
     1681 cacctggcgc tgtttcatca gcgcagccat acgtacggcg ttacgtgcgt attccacgaa
     1741 catcaggaag gtggaggtgt acggcaggaa gccaccgtgc agggagatac cgttagcaat
     1801 cgcggtcata ccgaactcgc gaacaccgta gtggatgtag ttacctgcag catcttcgtt
     1861 gattgcttta gaaccagacc acagggtcag gttagacggt gccaggtcag cagagccgcc
     1921 gaggaattca ggcaacagcg ggccgaacgc ttcgatagca ttctgcgacg ctttacggct
     1981 ggcgattttc gccggattag cctgcagttt agcgataaac tctttcgctt tggcgtcgaa
     2041 gtcagacggc atttcgcctt tcatacggcg ggtaaattca gccgcttcct gcggataagc
     2101 tttcgcgtaa gccgcaaact tctcattcca tgcagattct ttcgcctggc ctgcttcttt
     2161 cgcatcccac tgagcataga tttcagacgg gatttcgaac ggtgcatatt tccagcccag
     2221 ctgttcgcgg gtcagggcaa tttcagcgtc gcccagcggc gcaccgtggg agtcgtgagt
     2281 accggctttg ttcggggaac cgaaaccgat gatggttttg cacatcagca gggacggttt
     2341 gtcagtcact gcgcgcgctt cttcaactgc gcgtttgatg gatgccgcgt catgaccgtc
     2401 gatgtcgcga ataacgtgcc agccgtaagc ttcgaaacgc attgctgtgt cgtcggtgaa
     2461 ccagccttca acgtgaccat cgatagaaat accgttgtca tcgtagaacg caatcagttt
     2521 acccagcttc agcgtacccg ccagagagca aacttcgtgg gagatgcctt ccatcatgca
     2581 gccgtcgccc atgaaggcgt aggtgtagtg gtcgacaatg tcgtggcccg gacggttaaa
     2641 ctgcgccgcc agcgtttttt ctgcaatcgc cataccgact gcgttggcaa taccctgacc
     2701 cagcggaccg gtggtggttt ccacaccagc ggtataacct acttccgggt ggcccggagt
     2761 tttagagtgc agctgacgga agtttttcag ttcttccatc ggcagatcgt aaccggtgag
     2821 gtgcagcagg ctgtagatca gcatggagcc gtggccgttg gacagcacga agcggtcacg
     2881 gtcagcccag gacggattct gcgggttgtg tttcaggaaa tcacgccaca ggacttcggc
     2941 aatgtcagcc atacccatag gggcacccgg gtgaccggat ttggctttct gtactgcgtc
     3001 catgctcagc gcacgaatag cattggcaag ctctttacgt gaggacattt tgactccaga
     3061 tcggatgatg aagggcacgc ccttaacgac ttgacgacag cgcgttttgg gctacgccgg
     3121 aaaatttgcc aacaatttac cgcaagccgc gcgtcatgta catggaacat ccttttgccg
     3181 cttcagaaat ctctggatca tgctcgcatg ttgcgcaatc tgctcgcccg tccgctgcgc
     3241 ttttccttat actgagactg agcgtcgatt cacctgcaaa cggcgcattt ttagaataat
     3301 cctgaccttg tgcggaagag aaaacatgaa aattcgcgcc ttattggtag caatgagcgt
     3361 ggcaacggta ctgaccggtt gccagaatat ggactccaac ggactgctct catcaggagc
     3421 ggaagctttt caggcttaca gtttgagtga tgcgcaggtg aaaaccctga gcgatcaggc
     3481 atgtcaggag atggacagca aggcgacgat tgcgccagcc aatagcgaat acgctaaacg
     3541 tctgacaact attgccaatg cgctaggcaa caatatcaac ggtcagccgg tgaattacaa
     3601 agtgtatatg gcgaaggatg tgaacgcctt tgcaatggct aacggctgta tccgcgtcta
     3661 tagcgggctg atggatatga tgacagataa cgaagtcgaa gcggtgatcg gtcacgagat
     3721 ggggcacgtg gcgttaggcc atgtgaagaa agggatgcag gtggcgcttg gtacaaatgc
     3781 cgtgcgagta gctgcggcct ctgcgggcgg gattgtcggc agtttatctc aatcacaact
     3841 tggtgatctg ggcgagaaat tagtcaattc gcaattctcc cagcgccagg aagcagaagc
     3901 cgatgattat tcttacgatc ttctgcacca acgcggcatc agcccggcag gtcttgccac
     3961 cagctttgaa aaactggcga agctggaaga aggccgtcaa agctcaatgt ttgacgacca
     4021 tcctgcatcc gccgaacgtg cccagcatat tcgcgatcgc atcagcgcgg atgggattaa
     4081 gtaaagcctg gtggtgtcga acgtaggtca gataaggcgt tcacgccgca tctgacatta
     4141 atggcacatt tgttaccttg tgcgcattgc cggatgcgat gctggcgcat cttatccggc
     4201 ctacgggtgc cgaacgtagg tcggataagg cgttcacgcc gcatccgaca ttaatggcac
     4261 gttttacccg tgcgcatcgc atctggtgct tactcgccct ttttcgccgc ctgaatatac
     4321 agcatctcca gcgccagcgt cgccgcagcc agagcggtga tttccgactg atcgtatgcc
     4381 ggagccactt ccactacgtc catcccaacg atgttgagat ctttcaggcc gcgtaccagt
     4441 ttaatggcgc gatcggaggt caggccgcca atcactggcg taccggttcc cggcgcgaaa
     4501 gcaggatcca ggcagtcgat atcaaaagtc aggtaaaccg gcatatcacc cacaatctgt
     4561 ttcacctggg cgataacgtc atccacgctg cgatcgttca cctggcaggc gtccagcacg
     4621 gtaaagccgt tgtctttatc aaactcggta cgaataccaa tctgcacgga atgattcggg
     4681 tcgatcagac cttctttcgg cgcggtatag aacatagtgc cgtggtcaaa ttcacaaccg
     4741 ttcgcatagg tatcggtgtg ggcgtcaaag tgtaccagcg ccattttgcc gaaatgcttc
     4801 gcatgagcac gcagcagcgg cagcgtaaca aagtggtcac caccgaagga gagcatacgc
     4861 ttaccggcag ccagcagctt ctcggcgtgc gcctgcagct tttcgctcat ctcacgggca
     4921 tcgccaaagg catataccag atcgccgcag tccacgacgt tcagacgctc acgcatgtcg
     4981 aaattccacg ggaagcggtt gtgttcccag gccagattcg tcgaaacctg acggatcgct
     5041 gccggaccgt ggcgaccacc cgcacgacca gaagtggcca tatcgaacgg cacgccagta
     5101 atcacc
//
LOCUS       DAJTXY010000200         5020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTXY010000200 DAJTXY010000000
VERSION     DAJTXY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.202"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            91..1206
                     /gene="adrA"
                     /locus_tag="OO566_003980"
                     /gene_synonym="dgcC"
     CDS             91..1206
                     /gene="adrA"
                     /locus_tag="OO566_003980"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6607044.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1223..2032)
                     /gene="proC"
                     /locus_tag="OO566_003981"
     CDS             complement(1223..2032)
                     /gene="proC"
                     /locus_tag="OO566_003981"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6607045.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2152..2610
                     /locus_tag="OO566_003982"
     CDS             2152..2610
                     /locus_tag="OO566_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6607046.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2793..3317
                     /gene="aroL"
                     /locus_tag="OO566_003983"
     CDS             2793..3317
                     /gene="aroL"
                     /locus_tag="OO566_003983"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6607047.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3367..3558
                     /gene="yaiA"
                     /locus_tag="OO566_003984"
     CDS             3367..3558
                     /gene="yaiA"
                     /locus_tag="OO566_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6607048.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3816..4493
                     /locus_tag="OO566_003985"
     CDS             3816..4493
                     /locus_tag="OO566_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6607049.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLEKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4565..4849
                     /gene="ppnP"
                     /locus_tag="OO566_003986"
     CDS             4565..4849
                     /gene="ppnP"
                     /locus_tag="OO566_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6607050.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1221 a         1193 c         1321 g         1285 t
ORIGIN      
        1 gctaacgggt gagctacgaa agtggctcac ccgaaatttc atacttctgc ctttagcccc
       61 gtctctataa tttgggaaaa ttgtttctga gtgttcccaa aaataatgaa tgatgaaaac
      121 tttttcaaaa aagcggcggc gcatggggag gaacctcctt taactcctca aaacgaacat
      181 cagcggtccg ggctgcgctt cgcccgtcgc gtcagactac cccgtgcggt tggcctggct
      241 ggcatgttct taccgattgc ttcaacgctg gtttcgcacc cgccgccggg ctggtggtgg
      301 ctggtgttgg tcggctgggc gttcgtctgg ccgcatttag cctggcagat agcgagcagg
      361 gccgtcgatc cgcttagccg ggaaatttac aacttaaaaa ccgatgcagt attagcggga
      421 atgtgggtag gcgtaatggg cgtaaacgtg ctgccttcca ccgctatgtt gatgattctg
      481 tgtctgaatt tgatgggggc aggcggcccc cgtctgtttg tcgcgggtct ggtgttgatg
      541 gtggtttcct gccttgtcac cctcgagctt acgggcatta ccgtgtcgtt caatagtgcg
      601 ccgctggaat ggtggctctc ccttcccatt attgtcgttt atcctctgct gtttggctgg
      661 gtcagctacc agacggcaac caaactggcg gaacataaac gcaggttgca ggtcatgagt
      721 actcgcgacg gcatgacggg cgtgtataac cgacgtcatt gggaaactat gttacgcaat
      781 gaatttgata actgtcggcg gcataatcgc gatgcaacgt tactgattat cgatatcgac
      841 catttcaaga gcatcaacga tacctggggt catgatgtgg gcgatgaagc gattgtggcg
      901 cttacccgac agttacaaat taccctgcgc ggtagcgatg tgattggtcg gtttggcggc
      961 gatgagtttg cagtaatcat gtccggtacg ccagctgaga gcgccattac cgccatgtta
     1021 cgggtgcgtg aagggctaaa cacgttacgc ttgccgaata cgccacaggt aactttacgg
     1081 attagtgtgg gggttgcgcc gctgaaccca caaatgagtc actatcgtga gtggttgaaa
     1141 tcggcagatt tggcgcttta caaagcaaag aaagccggac gtaaccgcac cgaagtggcg
     1201 gcctgacgtc cggcgaaagt catcaggatt tgctgagttt ttctgatttt tccatacact
     1261 tcgtcatcgc ttcgatcact gcggcacgga agcctttctc ttccagtacg cgtaccgctt
     1321 caatggtggt acctcccggt gagcagacca tatctttcag tgcccccgga tgttctcccg
     1381 tttccagcac catttttgcg gaacccatta ccgcctgagc ggcaaattta tacgcctgga
     1441 cgcgtggcat cccgcccagc acggcggcgt cggccatcgc ttcgataaac ataaatacgt
     1501 aggctggcga agaaccgctc acaccgacca ccgggtggat catcggctca gcaattactt
     1561 ccgcttcgcc aaagcagcgg aaaatattca gcacatcagc ggtatcttct ggggttacca
     1621 gcgcgtttgg cgttacggag gtcatcccgg cattaaccag cgcgggagtg ttcggcatgg
     1681 cgcggataat tttccggtca tggcccagcg cgcgggcaag ctggtcgagc gtgacacctg
     1741 cagcaataga aacgaccaga gagtctttat tcaggctgga ggtgatttcg ctaagcactt
     1801 taatcatgat gccaggttta acggcagcaa aaatgatgtc ggcgatttgc gccacttctt
     1861 gcgccgattc tgcggcgttg atgccgaact ggtcatgcag ggcggcgact ttatccgggg
     1921 agggggtgta tacccagatt tgccctggaa gcacctgacc gctggcaatt agaccgccga
     1981 gaatggcttt tcccatattg ccgcagccaa taaaaccgat tttcttttcc attgcctcac
     2041 tcctgccgtg aaattcattg ttttgataat cgctggcaga agcataaaca gaactatgcc
     2101 ggaaggcaaa agcgcgacac aatagaggat tacccaacaa aggatgactt tatgacaatt
     2161 tgggtggatg ccgacgcgtg tcccaatgta attaaagaga ttttgtatcg cgcggcggaa
     2221 cgtatgcaga tgccgctggt actggtagca aaccagagtt tacgcgtgcc gccatcgcga
     2281 tttattcgta cgctgcgcgt cgcggcaggt ttcgacgttg ccgataacga aattgtccgg
     2341 cagtgtgaag cgggcgattt ggtgatcacc gcagatatac ctttggctgc tgaagccatc
     2401 gagaaaggcg cagcggcgct taatccgcgc ggcgaacgtt acacgccagc gaccattcgt
     2461 gagcgcctga cgatgcgcga ttttatggat accttacgtg ccagtgggat ccagaccggc
     2521 ggaccagata gcctttcaca acgtgaccgc caggcctttg ccgcggagct ggagaagtgg
     2581 tggctggaag tgcaacgtag tcgtggctaa atgtaattta ttatttacac ttcattcttg
     2641 aatatttatt ggtatagtaa gggatgtatt gagattttca ctttaagtgg aattttttct
     2701 ttacaatcga aattgtacta gtttgatggt atgatcgcta ttctcatgac accggctttc
     2761 gccgcattgc gacctattgg ggaaaaccca cgatgacaca acctcttttt ctgatcgggc
     2821 ctcggggctg tggtaaaaca acggtcggaa tggcccttgc cgattcgctt aaccgtcggt
     2881 ttgtcgatac cgatcagtgg ttgcaatcac agctcaatat gacggtcgcg gagatcgtcg
     2941 aaagggaaga gtgggcggga tttcgcgcca gagaaacggc ggcgctggaa gcggtaactg
     3001 cgccatccac cgttatcgct acaggcggcg gcattattct gacggaattt aatcgtcact
     3061 tcatgcaaaa taacgggatc gtggtttatt tgtgtgcgcc agtatcagtc ctggttaacc
     3121 gactgcaagc tgcaccggaa gaagatttac ggccaacctt aacgggaaaa ccgctgagcg
     3181 aagaagttca ggaagtgctg gaagaacgcg atgcgctata tcgcgaagtt gcgcatatta
     3241 tcatcgacgc aacaaacgaa cccaaccagg tgatttctga aattcgtagc gccctggcac
     3301 agacgatcaa ttgttgattt tagagcgcct atacttaacg ttcatcccgt gaaataagga
     3361 agaacgatgc caacgaaacc gccttatcct cgtgaagcat atatagtgac gattgaaaaa
     3421 ggaaagccag gacagacggt aacctggtac caactcagag ccgatcatcc taaaccagac
     3481 tcgttgatca gtgaacatcc gacggctcag gaagcgatgg atgcgaaaaa acgctatgag
     3541 gaccctgaca aagagtgacc gcatcagact gctcggaagg gattctgagt gccactacaa
     3601 gggatctgcg tcacattttt cataattcat gtttttctaa taattagaat attaaacaat
     3661 aacattccat tactggaatc atttggaatc tttacattat gccgtgcacg tctgctgcta
     3721 cgctttttgt catttgtagc acaagtaagt gtcagcagtg gtgcttcaca cttgcccggt
     3781 aattaacgac gaaagaaaag taaggtggat gaacaatgag tgcgtcgttg gcgatcctga
     3841 ccatcggcat tgtacctatg caggaggttt tgccgctcct gacggaatac attgacgaag
     3901 ataatatttc ccatcatagc ctgctggaga agttaagtcg tgaagaagtg atggcggagt
     3961 acgcgccaga agcaggcgaa gacaccattc tcacattatt aaatgacaac cagctggccc
     4021 atgtttcgcg tcgtaaagtg gagcgagatc tgcaaggtgt ggttgaagtg ctcgataatc
     4081 agggttatga cgtcatttta ttaatgagta cagcaaacat tagtagtatg actgcgcgta
     4141 atacgatctt tcttgagccg tcgcgaatat tgcctccact ggtttcctct attgttgaag
     4201 atcatcaggt gggggttatc gttccggttg aggagatgct gcccgttcag gcgcaaaaat
     4261 ggcaaatttt gcagaaatcg ccggtatttt cattgggtaa ccccattcat gattcagaac
     4321 aaaaaatcat tgatgctggg aaagaattac tggcaaaagg ggctgatgtc atcatgctgg
     4381 attgtttagg attccaccaa cgtcatcgcg atttactgca aaaacagctc gatgttcctg
     4441 tcttgctctc taacgtattg attgcacggc tggctgcgga attactggtg taattttgcg
     4501 tgacagccag cgcctctggc ccctatagtg aagtagatgt tcaactacca gacagggcca
     4561 gtttatgctt caaagtaatg agtacttttc cggcaaagtg aaatcgatcg gcttttccag
     4621 cagcagcact ggtcgcgcca gcgtgggtgt tatggttgaa ggcgaataca ccttcagcac
     4681 cgctgagccg gaagagatga cggtaatcag tggcgcgctg aatgtgttac tgcctgacgc
     4741 gaccgactgg caagtgtatg aagccggttc ggtgtttaat gttcccggtc acagtgagtt
     4801 tcatcttcaa gttgccgaac ctacctctta tctgtgccgc tatctgtaat tcctcgcctt
     4861 ccccttgaac gggagggcat ttttctgaaa tatcctctct ttaacccata ataatatttc
     4921 ctttgctgcg attttttcaa tttccgatat attcataatt catcaaggtt gatataaata
     4981 tcagtgaaga tctccagggt aatgactcca acttactgat
//
LOCUS       DAJTXY010000201         5014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTXY010000201 DAJTXY010000000
VERSION     DAJTXY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.203"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..236
                     /locus_tag="OO566_003987"
     CDS             <1..236
                     /locus_tag="OO566_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607051.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            276..816
                     /locus_tag="OO566_003988"
                     /pseudo
     CDS             276..816
                     /locus_tag="OO566_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            883..2301
                     /gene="dcm"
                     /locus_tag="OO566_003989"
     CDS             883..2301
                     /gene="dcm"
                     /locus_tag="OO566_003989"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6607052.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2282..2752
                     /gene="vsr"
                     /locus_tag="OO566_003990"
     CDS             2282..2752
                     /gene="vsr"
                     /locus_tag="OO566_003990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6607053.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="OO566_003991"
     CDS             complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="OO566_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6607054.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3834..4751
                     /locus_tag="OO566_003992"
     CDS             3834..4751
                     /locus_tag="OO566_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6607055.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGTIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4830..5012
                     /locus_tag="OO566_003993"
     CDS             4830..5012
                     /locus_tag="OO566_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6607056.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1225 a         1199 c         1437 g         1153 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtc ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gcgactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcac
      301 agttcgagaa ctggttgaaa aaccatcatc aacatcagga cgcggcgcat gacctgtgtc
      361 attttcgccg tgtctgggct acggcacaga aactggcggc agatgacgat gtcgatatgc
      421 tggtgatttt aaccgcctgt tattttcacg atatcgtgag cctagcgaaa aatcatccgc
      481 aacggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      541 tcgtgcaatt tccggcggag aaaattgagg ccgtttgtca tgccattgcc gctcacagtt
      601 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
      661 tggaagccct gggggcgatt ggcctggcgc gcgtatttgc cgtttcaggg gcattgagct
      721 tggcgctgtt tgatggcgaa gctccgtgcc gaactggcgg gggagaatga aggtatcgat
      781 cacaaggtga tagatgcgtt ttcacccgct ggctgagcgc gtggccctaa acggcagtaa
      841 ttatgttaac ctgtcggcca tctcagatgg ccggttaaat ctatgcagga aaatatatca
      901 gtaaccgatt catacagcac cgggaatgcc gcacaggcaa tgctggagaa actgctgcaa
      961 atttatgatg ttaaaatgct ggtggcgcag cttaatggtg tgggtgagaa tcactggagc
     1021 gcggcaattt taaaacgtgc gctggcgaat gactcggcat ggcaccgttt aagtgagaaa
     1081 gagtttgccc atctgcaaac gttgttaccc aaaccaccgg aacatcatcc gcattatgcg
     1141 tttcgcttta tcgatctatt tgccggaatt ggcggcatcc gtcgtggttt tgaatcgatt
     1201 ggtggacagt gcgtgtttac cagcgaatgg aacaaacatg cggtacgcac ttataaagcc
     1261 aaccattatt gcgacccggc gacgcatcat tttaatgaag atatccgcga catcaccctc
     1321 agtcatcaag aaggcgtgag tgatgaggcg gcggcggaac atattcgtca acacattcct
     1381 gaacacgatg ttttactggc cggtttccct tgtcagccat tttcgctggc tggcgtatcg
     1441 aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg atacccaggg cacgctgttc
     1501 tttgatgtgg tgcgcattat cgacgcgcgt cgtccggcga tgtttgtgct cgaaaacgtc
     1561 aaaaacctga agagtcacga taagggaaaa acgttccgca tcatcatgca gacgctggac
     1621 gaactgggct atgacgttgc cgatgcagaa gataacgggc cggacgatcc gaaaatcatc
     1681 gatggtaaac atttcctgcc gcagcaccgt gaacgcatcg tgctggtggg ttttcgtcgc
     1741 gatctgaatc tgaaagccga ttttactctg cgtgatatca gcgaatgttt ccctgcacag
     1801 cgagtgacgc tggcgcagct gctggacccg atggttgagg cgaaatatat cctgacgccg
     1861 gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc aggcgcgcgg taacggcttc
     1921 ggttatggaa tggtttatcc gaacaatccg caaagcgtca cccgtacgct gtctgctcgt
     1981 tattacaaag atggcgcgga aattttaatc gatcgcggtt gggatatggc cacgggtgag
     2041 aaagactttg acgatccgct gaatcagcaa catcgtccac gtcggttaac gcctcgtgaa
     2101 tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga aattccgcat tccggtttcg
     2161 gacactcagg cctatcgcca gttcggcaac tcggtggtcg tgccggtctt tgccgcggtg
     2221 gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt tgcgtcagca agaggcacaa
     2281 catggtcgac gttcacgata aggccacccg cagcaaaaat atgcgcgcga ttgccacgcg
     2341 tgatacggcg attgagaagc gcctcgccag tttgttaacc gggcagggcc ttgcgtttcg
     2401 cgttcaggac gccagtctgc ccggacgccc ggattttgtc gttgatgaat atcgctgcgt
     2461 gatatttacc cacggctgct tctggcatca tcatcactgc tatctgttta aagtgcctgc
     2521 gactcgaact gagttctggc tggagaagat aggtaaaaat gttgaacgcg atcgccgcga
     2581 tatcagtcgc ttgcaggagc tcggctggcg cgtactgatt gtctgggagt gtgcgttacg
     2641 taggcgcgag aagctgacgg atgcggcgct taccgagcgt ctggaagagt ggatctgcgg
     2701 cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat ttactcgctt gatgcatcct
     2761 gaataactgg cgcgacaacg ggtttggccg ggaagagata ttttcccaac gtgaccagta
     2821 ccaccgcgaa aacaattacg ccgagcgcca gccattcaat cttcgacagc gtttcgccac
     2881 ccagtcccgt acccagcaag accgcgacca ccgggttaac gtaggcgtag ctggtggcga
     2941 gagccggact gacattacgg attaaataca tataagcgtt aatggcgata atcgaaccaa
     3001 acagcgccag atagccgacc gcaaggaagc ctgacaggga agggagcgcc gtcagttttt
     3061 cacccgcaat catcgatgcg atcattaaca ccacgcctgc cgccagcatc tcaatcgcac
     3121 ccgccatcat ccctacaggt aaggtaatgc gcgagccata aactgagcca aacgcccagc
     3181 taatcgagcc gattaaaatc agaatcgcgc cccacggatt gccgcttaaa tttccaccgc
     3241 tattgagcat gatgattccg gcaagcccaa tggcaatacc cacccattcc agtttgcgcg
     3301 ttttaatgcc aaacaggcga ctgaagcaca gggtaaagag gggcacggtt gcaatcacta
     3361 cggcggcgat gccggaagga acattttgat gttcggcaac cgtcaccatg ccattaccga
     3421 cagccagcaa taacaggcca atcagcgcgg cattgagcag cggacgtagc gggggaagtt
     3481 tgtgtccgcg cagtagcaaa aatgccagca ataaaatacc ggctgccagg aatcgaacgc
     3541 ccgccatcat taacggaggc cagcttccca cgccaatccg aatgacaaaa taggttgagc
     3601 cccaaatgat atacaacgca aacagcgcgc caaaaagcgg taacaactgg cggaaacgca
     3661 taatccctca cggcggaaat aaaaaggtgg ttcatagtaa acgtgaaaat cattctgctg
     3721 gctagagata taattgcact tgattgttaa ataaatgttg acctgtgaag caagtatcag
     3781 agctgccgtt tttgcttcat acttacaccc ttcagcaata aaaatgagag ggaatgcttt
     3841 tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg gacgatatct
     3901 ctgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat gatttatcgc
     3961 tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg gtctggggcg
     4021 tggcgaaagg atcgctgatc aataaagtga ttctggtgcc gctggcgctg atcatcagtg
     4081 cgtttatccc gtgggcgatt acgccactgt tgatgattgg tggcgcgttt ctctgctttg
     4141 aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa gatccagcgc
     4201 agagccagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt gaaaaggaca
     4261 aaattaaagg gacgattcgt accgatttta ttttgtctgc ggaaatcgtc gccatcacgc
     4321 tggggattgt ggcagaggcg ccgttgctta atcaggtgct ggtgctttca ggcatcgcgc
     4381 tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt gatgacctag
     4441 ggtattggct ggcggaaaaa tccagcgcgc tgatgcaggc attaggtaaa gggttattga
     4501 ttatcgcgcc ttggctgatg aaagcattat cgattgtcgg cacgctggcg atgttcctcg
     4561 tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgctatt gaacatatcg
     4621 ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat ctgattcttg
     4681 gatttatcat cggcggcatc gtggtgctgg gagtgaaagc agtagcgaaa atgcgcggtc
     4741 aggcacatta atactcgcac tggctattat gcaaaattcg ccatcttcaa ctaaggtgaa
     4801 caagtttcac tcgaaaaaag gaggcgagta tgagctttat ggttagtgag gaagttacgg
     4861 ttaaagaggg cggcccgcga atgattgtca ctggatactc cagcggtatg gttgagtgtc
     4921 gttggtatga cggttacggg gtcaagcggg aagcttttca tgaaaccgat cttgttccgg
     4981 gggaggggag tcgttctgcg gaagaagttt gaaa
//
LOCUS       DAJTXY010000202         4974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTXY010000202 DAJTXY010000000
VERSION     DAJTXY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.204"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..171)
                     /locus_tag="OO566_003994"
     CDS             complement(<1..171)
                     /locus_tag="OO566_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR6607057.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIAL"
     gene            complement(235..2064)
                     /gene="recQ"
                     /locus_tag="OO566_003995"
     CDS             complement(235..2064)
                     /gene="recQ"
                     /locus_tag="OO566_003995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6607058.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLLDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(2197..3066)
                     /gene="pldA"
                     /locus_tag="OO566_003996"
     CDS             complement(2197..3066)
                     /gene="pldA"
                     /locus_tag="OO566_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6607059.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3231..3698
                     /locus_tag="OO566_003997"
     CDS             3231..3698
                     /locus_tag="OO566_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6607060.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3750..4658
                     /gene="rarD"
                     /locus_tag="OO566_003998"
     CDS             3750..4658
                     /gene="rarD"
                     /locus_tag="OO566_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6607061.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSKSKINIF"
     gene            4699..>4974
                     /locus_tag="OO566_003999"
     CDS             4699..>4974
                     /locus_tag="OO566_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607062.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTL"
BASE COUNT         1183 a         1283 c         1237 g         1271 t
ORIGIN      
        1 caacgcaatc ccagcccaaa ccattacgcc gcaggtaatg cccagcacgc ccatcatcgc
       61 ttctttacgg gaacgactga cagcggtctg agagacaaaa aagaaatcgg gaccggggct
      121 cataagcgcc acaatgtgca ccatggcgac ggtgagaaat aacatcaaca tacatttgac
      181 tcgcggggga aacgtttacg gagtcttcat actggcactt ttttatgctg ctgactactc
      241 ttcgtcgtcg ccatcgacat gcgcacgaat cagcgccata aacggtttac caaagcgttc
      301 cagcttgcgc atcccaacgc cgttaacgct gagcatttcg ctggcggtga tcggcatctg
      361 ttcagccatc tcaatcaagg ttgcgtcgtt aaacaccacg tacggcggga cattactttc
      421 atcggcaatc gatttacgca gtttgcgtaa tttggcgaac agtttgcgat catagttgcc
      481 gccgaacgat ttctgcatcg ctttcggttt gagcgccacg atacgcggca cggcaagttg
      541 caaagaggat tcgccgcgca gcaccgggcg cgcggcctct gtcagttgta gggcagaatg
      601 ctgggcaata ttttgcgtca ccaggccgag atgaatcagc tggcggatca cgctcaccca
      661 atgttcatgg cttttatcac ggcccatacc atagactttc agtttgtcat gaccatagtc
      721 gcggatacgc tggttattag caccacgaat cacttccacc acataaccca tcccaaaccg
      781 ctgattcaca cgaccaatgg tggaaagggc aatctgagca tcggttgaac cgtcgtactg
      841 tttcggcgga tcgaggcaga tatcgcagtt cccgcacggc tcctgacgcc cttcgccaaa
      901 atagttcagc aataccagac gacggcaagt ttgcgcttcg gcaaacgcgc ccatcgcatt
      961 gagtttgtgg cgctcgatat ccagcaactg cccctgcggc ttctcttcca gacaacggcg
     1021 cagccacgcc atatcagccg gatcgtaaaa cagcatcgct tccgcaggca ggccatcacg
     1081 cccggcgcga ccggtttcct gataatagga ttcgatattg cgcggaatat caaagtggac
     1141 cacgaagcga acgtttggtt tattgatgcc catgccgaac gccaccgtcg ccaccacaat
     1201 ttgcagatca tcgcgctgga atttctcctg cacgtcggcg cggacattat tttccagccc
     1261 ggcatgatag gccgccgcgc taataccctt gctttgcagg cgcgcagcgg tgtcttctac
     1321 tttcgcccgg ctgttgcagt agataatgcc agacttaccg cgctgttcct gcacgtagcg
     1381 catcaactga tcgagcggtt tgaatttctc catcagcatg tagcgaatat tcggacggtc
     1441 aaaactgctg atttgaatca gcggatcgtt cagccccagc aggcgcacga tatcctggcg
     1501 cgtggtgtcg tcggctgtgg cggtcagcgc cataaacggc agcgtcggga accgctggcg
     1561 caactgaccg agcgcggcat attccgggcg gaaatcgtgg ccccattggg agatacagtg
     1621 cgcttcatcg acggctaata acaccggatt ccagtgcgcc agatgctcaa gaaagttatc
     1681 cagcatcagg cgttccgggg cgatatacag taagcgaatt tgcccggtgc ggcagcctgt
     1741 catcacttca agttgctgtt cgcgggtttg cgtcgagtta aggcacgccg ccgccacgcc
     1801 gttggcctgt agttgatcca cctgatcttt catcaacgaa atcagcggtg aaacaaccac
     1861 ggtaaggccg tttagcaata aggcaggaat ttgatagcaa agggattttc cgccaccagt
     1921 gggcataaca accaggcaat cgcgaccgga aagcacagtg tcgataattt cttcctggcc
     1981 ggggcgaaac tgttggtagc caaaggtttc ttgtaaaacc tgtttagctc cggactccag
     2041 attcaacact tccgcctgcg ccacattcac cccgattgcc tgaaataaaa acaggcgcta
     2101 ttttcagcgc ctgcgcgaga aactgcaatg cttttagtct ctgagagggt aatcctggat
     2161 gctggattcc agatttgcca accactcaac cgttcatcaa aacaagtcgt ttagcataac
     2221 ccctacaccg acacgggtct ggttgaagtt atagtcgatg agcgattctc catagccgct
     2281 gtaaacctga gtataaaggc gcacatgttt ggtgatcggg taacttaagc ctaactccgc
     2341 gccgccgtag ccggtgttcc agttgtactg tcctttcgca ctgagcaccg catcaccgag
     2401 gtgatagccg attttaagct ggtagtaacc catatatttg gtgatatccg ggttatcgtc
     2461 agtattaccc accacatacc acggcttcac ttctaccagc cagttaccgt tttctgccat
     2521 caggcgagta taaaggcggt tccagctgcg ggaggtcggg tctgaacgcc cgttagagtc
     2581 gtggttatac cccatctcca catcgcgcag cgtccaacct gcaaaacggt aatcggtggc
     2641 aaaaccgagg aacaattgcg gttcgtagtt ggtttcacga aacggtgaag actcttcgct
     2701 attggacagt tgccaccagg atttttgcgt ataagacgca cccaacaccg agtttgggcc
     2761 taaaatccca cgccacagcg gaaatgccag gctcaactga aactttactt catccttacg
     2821 cgcattttcc gcccagtcgt aactggcaat cgcttcttta ttcagatcgc tggtttgggt
     2881 gtagatgagg tagttggtgt cataaggata gagcgtgaac ggattgtcat gctcctgcag
     2941 catattggcg ataatactgc cacgcaccgc tggcgcgtca tgcacctctt tcaccgttgc
     3001 ctcttgtgca tatactgcca taggcaacat aaacaccggc aacaaccagc cctgcagagt
     3061 ccgcatcggt cgtgttctcc gtaacgaatt attgataagg tgaatgtttt tctgcgggct
     3121 attttacata cttaactcca ttgccgttag ttatcccttc ctaaagtaga aaccaacaac
     3181 aaagaagcat aaaatcaaca tttcattaac caatgaagtg taaggatgat atgtctgccg
     3241 tactgaccgc tgaacaagcc ctgaaattag tgggtgagat gtttgtttat cacatgccat
     3301 ttaaccgcgc attggggatg gaactggagc gttacgaaaa agagttcgca cagctggcct
     3361 ttaaaaatca gccgatgatg gtgggtaact gggcgcaaag cattttgcac ggcggagtca
     3421 ttgcgtcggc gctggatgtc gccgccggtc tggtgtgcgt gggaagtacc ttaacccgcc
     3481 acgaaaccat cagtgaagat gaactacgcc agcggctatc gcggatgggg accattgatc
     3541 ttcgcgttga ttatctgcgc ccaggcagag gcgagcgttt tactgctacc agtagcctgt
     3601 tgcgtgcagg caataaagtc gccgtcgccc gcgttgaatt acacaatgaa gaacagcttt
     3661 atattgccag tgccaccgcc acctatatgg taggttgaag acataaaagc aggtaaactg
     3721 ctgttaccaa ttaaaaatga gtattcccga tggatgcaaa acaaacgcgg cagggcgtat
     3781 tactcgctct tgccgcttat tttatttggg gtattgcgcc agcatacttc aagctgattt
     3841 actacgtgcc cgccgatgag atcctgacgc atcgcgtgat ctggtcgttt ttctttatgg
     3901 tggtgctgat gagcatttgc cgccagtggt cctatttaaa aacgctgatt cagacgccac
     3961 agaaaatttt tatgctggca gtctctgccg tgctgattgg tggcaactgg ctactgttta
     4021 tctgggcggt gaacaatcac catatgctgg aagcgagcct tggttacttt attaacccgc
     4081 tggtgaacat tgtgctgggg atgattttcc tcggcgagcg attccgccgg atgcaatggc
     4141 tggcggtgat tctggcgata tgtggcgtgt tggtccagct gtggactttt ggttcgctac
     4201 ctattatcgc gctggggctg gcatttagtt ttgccttcta cggcctggtg cgcaagaaga
     4261 ttgcggtaga agcacaaacc ggcatgttaa ttgaaaccat gtggctgctg cccgtggcgg
     4321 cgatttacct gtttgctatt gccgacagtt caaccagcca tatggggcaa aacccgatgt
     4381 cgctgaattt actgctgatc gccgccggta ttgtcactac cgtaccgctg ttgtgtttta
     4441 ctgccgctgc cacgcgcctg cgtctctcaa cgttaggctt ttttcagtac attggcccga
     4501 cgctgatgtt cctgctggct gtgacgtttt atggtgaaaa accgggtgcc gataagatgg
     4561 tgactttcac ctttatttgg gtggcgctgg caatttttgt gatggacgcg atttatacgc
     4621 agcgtagaac gtccaaatca aaaataaata tattctgaaa aaatatgccg actcttgtat
     4681 gagagtgggc atattgccat gctgacagga gactcacaca aggatgtaaa atttatgtta
     4741 cgtatgttta tcccaacatc taacggtaag atttccagac gccgatatat attttcattt
     4801 attttaataa atttcatttt tgcctttctt attatctttt ttaacgatgg ggaagcaggt
     4861 tttcttgtta tagtctctac catcgcactc cattatctcg tcatcaacat gaattgccaa
     4921 cgactccgtg atagtggttt catatatata aaaatttatg tttttggtac atta
//
LOCUS       DAJTXY010000203         4708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTXY010000203 DAJTXY010000000
VERSION     DAJTXY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.205"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..1139)
                     /locus_tag="OO566_004000"
     CDS             complement(<1..1139)
                     /locus_tag="OO566_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6607063.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGG"
     gene            complement(1139..1321)
                     /locus_tag="OO566_004001"
     CDS             complement(1139..1321)
                     /locus_tag="OO566_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607064.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(1333..3090)
                     /locus_tag="OO566_004002"
     CDS             complement(1333..3090)
                     /locus_tag="OO566_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6607065.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSAGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(3090..3572)
                     /locus_tag="OO566_004003"
     CDS             complement(3090..3572)
                     /locus_tag="OO566_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6607066.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(3720..4070)
                     /locus_tag="OO566_004004"
     CDS             complement(3720..4070)
                     /locus_tag="OO566_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6607067.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4113..4577)
                     /locus_tag="OO566_004005"
     CDS             complement(4113..4577)
                     /locus_tag="OO566_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6607068.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4574..4708)
                     /gene="rrrD"
                     /locus_tag="OO566_004006"
                     /pseudo
     CDS             complement(4574..4708)
                     /gene="rrrD"
                     /locus_tag="OO566_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
BASE COUNT         1054 a         1398 c         1116 g         1140 t
ORIGIN      
        1 ccaccgggac gcggattcat atcttccagg tcgcggcagt cattgggaga gtaaatcccc
       61 cagttaatcc cggtggcgta ggcttcaaaa cgggatttca tatccccgcg cagtaacgcc
      121 ccggcgttaa atttggcgta aaacttcccc tgtttgctct ccctgaccag ccctgtattg
      181 atccgttgtt caatacgggt cagatacggc acaagggaat agttgataaa tccgagcccc
      241 agctcttcaa tattgttgaa agtggcgcga tcggtgttct gcaccatgtg cagcggcacg
      301 cggaaaagac gacagatttc ttccaactga aacttgcggg tttccaggaa ctggctgtcc
      361 tcggcattca gtgccaccgg cttccagtcc agccccattt ccagaatcat cggacggtgc
      421 gcattaccca gcccaagatg acgctcctca aaatccctct tcatgcgctc ataagcatcc
      481 ggcgtgagct tttgttccgt gcgcaacaca ccggatgtca ccgcaccatt accaaacaac
      541 ctggcaccgt gctcctcggt tgccgctgcc agtgaaatgg cctcgcgcgc atatgcaatg
      601 ggattcagcc cgaccagtcc gtccagcgtc agggtgcgca catgccagat ttcatcctgg
      661 gtcaacacat ccacggaacc atccggaaac gtcacctgat aaaccggctg ccactggctg
      721 ttcagcttcg gttccacaca gcccggatct atcggaagaa gctccacaac ttcccccagc
      781 gccttcacct tgtaagcgta aaaattcccc cgcagacaca ggcagacgat aaccagctcc
      841 cagaattcct gcggcgtcat gtagccattg ggttttgctg aaatcagctt atgcagccgt
      901 tcatccaccg cccgtgtttt aagggtgccg ctgattttgt agagactgca gggcagcata
      961 ccaacagact cagccagcac cctgacgcag gaatagaccg ccgtcagccg catggcccgc
     1021 tggctgctga tccgctttcc ggtataggtg tcgtatgaca accccagctc ttccgcaagc
     1081 atcccgggcg ttgtgacggg ggtgttattt ttgcgttgaa aaagcccctg gaaaaacatt
     1141 actcacctcc ggaggcgacc cggtgaccgc gatcgagata acgcgcaacc agccacgacc
     1201 agcacagaca cagcgccccg gcaacaacaa aacctgccgg gggataaatc agccatgtgc
     1261 catacgacag caaaagcgcc cccagcacgc ccaccagtgg cgtgagaatt atcagaaaca
     1321 taatgacctc ggttaaagcg agcgaatacc aacgctgacc agatgctcag acagatccgg
     1381 ctccggttca ccgccattga ccagcatccg gctcattgct gtaaacatcg caacagggcc
     1441 gtcgattttg gcttccggcg tggatttgtt cgggaagata ttgtcgtttt tgtccggttt
     1501 caccgtaacg ttagacatca tccagttcat gaccgggtga ttactgtgat ggaaacgccc
     1561 ggcatagacc agtgattccg tttccttcat ggcctctgac agattgcgga ccgtctgcgg
     1621 aacttccacc agcggtatac cttcttcagc cagcgccaga ctgaactgca ttgcgctcca
     1681 cgggtcaaat cccagttccc tcaggttttc accgccaatc cattccagta agtcactttt
     1741 tatctgagca tgatcgataa catcaccatc cgtcagaatc agcttatcca tctccgccca
     1801 cttccggtaa agttctgcct gctgccgcga gcatcgttcc agccgtcctt ccggaagcca
     1861 gaatttaaaa tcggcatgaa catgcccgtt atccgttcgc cagagttttg ccgccgcaca
     1921 gatatcaatc ttatgagcaa ggtcaacgcc gacccacatg ggatacgttt tcagctcatg
     1981 tcgtggggca atgtattcgc acttctccca cttaatcatg tccatccagg cagactctgc
     2041 tgttacccac acattcatgt gtttggtaaa aaaattcacc cgcgcagaga cctgttcttt
     2101 cgcttttttc gccaggcgac gcagatcatc ccagcgttta cagatgccca ggccgggatt
     2161 cgctttctgc cagaccgttt catcaaacgg atcatctccc tcatcgaggg tgtaaataat
     2221 cgcaaagtag gagtcgtctt ttaccgcgcc ctccacgtcg ctgttatagc cacgcaatac
     2281 cttgatggcg taatcacgct gctcgtaaca aattccttcc ttgttaaacc ctgccgtggt
     2341 gataccaaat aaaagggact gcagacgggc accggttgcc gtttccagaa cgtcccacac
     2401 gtcacgggtt ttatgtgcat gcagctcatc aataatggcg cagtggatgt tcagaccatc
     2461 caggttgttt gcatctgaag aaagcggttc aaattttgat gcgctctgct cctgataaat
     2521 cgccagcttg ttgaaatcaa acaaccgccc gagtgtcgac cgggcttttc tgaccatatt
     2581 tttggcgtct tcaaacacga ttctggcctg gtcacgcgtg gttgcggctg aatacacctc
     2641 agccccgcct tcaccatctg cccccgtcat atacagaccg atacctgctg acagggttga
     2701 ttttgcgttt ttacgggcga cttcgttgta cgctgtccgg aaacggcgca ccatcaccgg
     2761 gcgtccgctg ccatcgctgc gcatgacaac ttccccggtc tcttcattca ccagcggaat
     2821 gacaaaacca aaaatattaa tgaggataaa cacatgccag tccatcaact caatgggctg
     2881 acctgccagc gcccctttta catggggcac aaatttgtag aaattcagga tgtgctgcgc
     2941 acggggttca ctgaaataaa tcccccgctc ttcgccgtac ttcagatcat caagaaaacg
     3001 ctggcaggcc agacggacaa attcaccagc gacaatttct cctgcaacaa cacgttcggc
     3061 gtagcggatc ccgtcagcca cttttgccat cagtctctcg cttttaaaag ttctgccagc
     3121 ggatcaacat catccggtcc ggcgatattt actttcgccc ggcttgccgg tgacatacca
     3181 aattctgcaa gcatcgcccg gatccgcttc caggcatccg ctttcatcgc agcagccggg
     3241 tgtgccttaa tcagcacatc accgttctgc gtttccgtgc ggtaggtata accctcaaca
     3301 tcgagggttt cgcagtgatg ccggtattcg gtgtaggctt ccaccagcaa ctcgagtgca
     3361 cgcgcatcaa gctgagaaat gatcccttcc gcattcagct cttccgccat tcgcctgaac
     3421 cagtactttc cctgcgcccc taaatgctgc ggaattttag ggagaccttt ttcgtccttt
     3481 ttagcggttt ttttggcgtc tttaacggga cgctttgagg gattgcctcg tatcaaatgc
     3541 aggcgtggcg gggttttcgg gggtcctgac ataatcgatt ttacctatca atcgcttgat
     3601 cgcattccca aaaaaaagtt ttcgaacctg cggcgatgcg aggaagggtt ggcgggcggt
     3661 cccggacagc cagggctgca gggatttgac ccgcccctcc ctacaagtga gaataattat
     3721 cacctgattc gttcgcgcgc tgttttcgct ttatggcagg gccagcacag actctgcagg
     3781 ttgctgtctg cgtctgttcc gccatgcgct ttcgggatga tgtggtcgac ggttttcgcc
     3841 tcgctcacca caccgacacg cagacacaac tgacacagac ctttatcgcg cttcagaata
     3901 cgggcacgaa tcaccgtcca ttttgagcca tagccacgct ggtggcggct cagtccgcgc
     3961 tggtgctgca cccagccttc accgcgatgc ttatcgcagt aacccgaact gtctgtggtt
     4021 gtacctgcac atccacgctt acggcaggca cgtgggatta gtgatggcat aaatacctca
     4081 taccctgcga aatgtttacc acgataaaaa ggctacttaa tgcactgagt gcggatatac
     4141 tcctgtgccc cttccagttg catctgcatc gtcatcagcc gctctctgag ggtgaaataa
     4201 tcccgttcag cggtgtctgc cagtcggggg ctggttgcat tatccacgcc ggaggcggtg
     4261 gtggcttcac gcactgactg acagactgct ttgatgtgca accgacgacg accagcggca
     4321 acatcagcgc gcagagtttc attttcagct ttcgcatcag ctaactcctt cgtgtattta
     4381 gcatcgagtg cattaacatc acgctgtctc tgctgcatgt cagtaatggt tgcgttcgcc
     4441 agcttcagtt ctcttgcgtt tttgtcgcgc tgctctttgt aggtaatagc gttatcacgg
     4501 taatgattaa cagcccatga caggcagacg atgatgcaga taaccagagc ggagataatc
     4561 gcggtgactc tgctcattgc tgcccccaca aacagacttc acgctcaatc tcacgacggg
     4621 tcatcagccc tttccattgc ttaccgccag catatgtcca gcgacgtagc tggtcacatg
     4681 cgcctttgat atcgccctag tttatttt
//
LOCUS       DAJTXY010000204         4578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTXY010000204 DAJTXY010000000
VERSION     DAJTXY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.206"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..213
                     /locus_tag="OO566_004007"
     CDS             <1..213
                     /locus_tag="OO566_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6607069.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="OO566_004008"
                     /pseudo
     CDS             356..577
                     /locus_tag="OO566_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(598..660)
                     /locus_tag="OO566_004009"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="OO566_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1110..2522
                     /gene="ospC1"
                     /locus_tag="OO566_004010"
     CDS             1110..2522
                     /gene="ospC1"
                     /locus_tag="OO566_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6607070.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2626..2775)
                     /locus_tag="OO566_004011"
                     /pseudo
     CDS             complement(2626..2775)
                     /locus_tag="OO566_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2851..4548
                     /gene="ospD3"
                     /locus_tag="OO566_004012"
     CDS             2851..4548
                     /gene="ospD3"
                     /locus_tag="OO566_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6607071.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1603 a          815 c          851 g         1309 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggaaatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg tgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccattaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact caaaaattgt aattcatacc ctgctatata ttgtagggaa agatattaat
     1081 taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg aactcagcaa
     1141 atacccaatg caatatagat tctatggata acagattaca tacattgttt ccaaaagtga
     1201 catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta aaagatagtg
     1261 caaatattat taaagatttc tttaggaaaa ctatagcagc acagagttat agtagaatgt
     1321 tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca tcagacgcta
     1381 aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat atatctgaaa
     1441 taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg ctaattatta
     1501 attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa attttaaaca
     1561 ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac acatacgctg
     1621 atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa atctctaacc
     1681 atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt gcaaatgcgt
     1741 atatcattga tcatgactct ccatatggat atatgacatt aaccgatcac tttgataatg
     1801 ctattccacc tgttttttac catgagcacc aatcattttt agataaattt tcagaggtta
     1861 ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta ccaatattca
     1921 atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt agaaaaagtg
     1981 aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg gatctggata
     2041 gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta agtacagaaa
     2101 acttcaaaaa agttaagatt agagaaatat ccttagagga ggctgttaca gcatctaatt
     2161 acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct ctttttcttt
     2221 cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt gagataacta
     2281 gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta cttagcgctc
     2341 ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt gatgtaaaca
     2401 caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata aaatatgaga
     2461 atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat aaatatattt
     2521 aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc gatatattgt
     2581 ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatggggt tcgccccgtt
     2641 gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga gcagcccccc
     2701 tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggg gacaaaccac
     2761 atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta agtctgcgtc
     2821 acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat cccatcaagg
     2881 aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac aatccagtct
     2941 ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt attaaatcta
     3001 aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt agcttcctac
     3061 tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt tgcttttccg
     3121 gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa tgtgcctgct
     3181 attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat tttcaatgaa
     3241 atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa tcacaatatg
     3301 gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat tcagctttat
     3361 gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa cttagctaaa
     3421 ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg ttatggtctt
     3481 atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat gtcctccata
     3541 atcagatggc ctaataattt acttcacccc aaagttattt atcacgcgat gcgtatggga
     3601 ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct ttcagacaat
     3661 acgttagaat tacttttggc agccaaaaat gacgatggtt tttcaggatt gcttttagct
     3721 ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga aacttctgga
     3781 cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg acgaatatcg
     3841 ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata cggaaggctt
     3901 ctcaagaaga gagcaataaa tatcgaatac aataagctga aaaatttgct gaccgcttat
     3961 tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca aaatggacat
     4021 gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct caactcagag
     4081 gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact tctgttagca
     4141 ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa tgaggcaaaa
     4201 cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg tttatctgga
     4261 ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg gaaaatactt
     4321 cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga aggcccaaat
     4381 ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat aaaaacttat
     4441 atgggaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca cttggacaaa
     4501 aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat attatctcgt
     4561 ggtgatacac tgaatttg
//
LOCUS       DAJTXY010000205         4577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTXY010000205 DAJTXY010000000
VERSION     DAJTXY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.207"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            437..2089
                     /locus_tag="OO566_004013"
     CDS             437..2089
                     /locus_tag="OO566_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6607072.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO566_004014"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO566_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO566_004015"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO566_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3030)
                     /locus_tag="OO566_004016"
     CDS             complement(2596..3030)
                     /locus_tag="OO566_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HCR6607073.1"
                     /translation="MDTFKRRNVATSFSFLGYDFKVRTLKNFKGELYRKCMPGASNAA
                     MCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFSYRLWSAMQSR
                     LLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYLLRASNE"
     gene            complement(3207..4516)
                     /gene="ltrA"
                     /locus_tag="OO566_004017"
                     /pseudo
     CDS             complement(3207..4516)
                     /gene="ltrA"
                     /locus_tag="OO566_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1217 a         1109 c         1055 g         1196 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggtacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgg
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagaaagg agaaactcgt
     3001 agcgacgttt ctccttttaa acgtgtcaat gtaggcgata ttcgtcttcc ctgcgttcag
     3061 gaccaacccg acttccgaga agcgcaccga taacctgttc ttcagccttg ttgcatcact
     3121 cattcgtgaa caatgtacta caagtcgagt cacccgaggg aacctccccc tctggtgctc
     3181 acggaaccgg acgtgaacct ctcagctcat ccggctcttc tcattgaggc ctgtccccat
     3241 agtaacggcc agtgtgcaaa taacctcgtt tcactccgcg caacacgggc cagccagtgg
     3301 ctggcccgtc gtcggtggcc cctaagtcgc ttgtgtttac ggggcagcca taacacgagt
     3361 ttgcgatcga tttgcctcag cgtcagatac agtgctgatt tataaaaagc accgtagtac
     3421 tttatccagc ctttgatttt cgcattgaac atatgcgcca gctcgtacaa ggacttgtca
     3481 ctgcgattct cctcacctcc tgacggattg cttttcctgc tctggcactc atcgccggac
     3541 tgaagttaac gaaggtctta ccccagcgat tcatcgacag acgcggccgg aatgtatagc
     3601 ccagaaagtt gaagctcgtt tccgggtagt ccccccgtcg gtcagcatcc ttacagtaaa
     3661 ctattcgtgt tttttgcgga tgcagctgca gcccgcattg cgcaagccgc tgcctcagtc
     3721 cacttatcag agcgtcagcc tgccactggc tgtgacagtg gcatacaaca tcatcggcgt
     3781 acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag gcatagtgca
     3841 ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct cgaccccgtg
     3901 ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca atgtacatca
     3961 ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca tgatcgatat
     4021 catcaaagaa gcctttaata tcgacttcca ccacccagtc aaacttccag cagcgtttcc
     4081 tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac gagtctgcat
     4141 ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg gcgatccggt
     4201 ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga atgtccacac
     4261 gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt ttgtagagat
     4321 tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct gccgccccac
     4381 ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca ggaaatggct
     4441 ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc gccgcaccat
     4501 cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc gctactacgg
     4561 tttactccgt ccctgta
//
LOCUS       DAJTXY010000206         4445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTXY010000206 DAJTXY010000000
VERSION     DAJTXY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.208"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..253
                     /locus_tag="OO566_004018"
                     /pseudo
     CDS             1..253
                     /locus_tag="OO566_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1492
                     /locus_tag="OO566_004019"
     CDS             746..1492
                     /locus_tag="OO566_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6607074.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1507..1929
                     /locus_tag="OO566_004020"
     CDS             1507..1929
                     /locus_tag="OO566_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6607075.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1987..2343
                     /locus_tag="OO566_004021"
     CDS             1987..2343
                     /locus_tag="OO566_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607076.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2436..2654
                     /locus_tag="OO566_004022"
     CDS             2436..2654
                     /locus_tag="OO566_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6607077.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2656..3018
                     /locus_tag="OO566_004023"
                     /pseudo
     CDS             2656..3018
                     /locus_tag="OO566_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3018..3254
                     /locus_tag="OO566_004024"
                     /pseudo
     CDS             3018..3254
                     /locus_tag="OO566_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3447..3683
                     /locus_tag="OO566_004025"
                     /pseudo
     CDS             3447..3683
                     /locus_tag="OO566_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3683..4048
                     /locus_tag="OO566_004026"
     CDS             3683..4048
                     /locus_tag="OO566_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6607078.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT         1186 a          945 c         1263 g         1051 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagatac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaattc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt
     1441 taactgggag agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga
     1501 ggatttatgg cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg
     1561 gaactcgtgc atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc
     1621 agtcgtgcgt ctgtcggtcg cttatgcatg gaactggtcg caagtggtga tgtatataat
     1681 tctggctacg gggtatttcc ctctgaacag gctcataaag actggcaaaa cgcccgcaaa
     1741 aagctatcga gggtaaaggt gaagaaaccg gttgtggttg atcctgacct tatctggtca
     1801 ttaccagacg gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc
     1861 cggaagagcg aagctatgca gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg
     1921 gtactgtgag taaaattagc tatcaggctt caattaccgc tggtattcgc atcaaaggag
     1981 aggagcatgg aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa
     2041 gaggatgacc cacgtaatca ggttaatttt ctttatgaac catcggaaag accatattgc
     2101 catcacgcct ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt
     2161 gcggttgtgg agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca
     2221 gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg
     2281 atacagattg agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa
     2341 taattaaatt tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca
     2401 aatcatcagg agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat
     2461 atccgcgaaa gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga
     2521 caccgatatc cccgctaatt tttgtctgga taatcggaaa aataattgag ccagttattg
     2581 aattgtataa cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt
     2641 ataaggaaag ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc
     2701 ggtggtcgct gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta
     2761 aaaccggtca ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac
     2821 atggtaccag aatgccaccc atgcaatctg cacaagcatt gcagtagcct agaagattat
     2881 cgacgaatta tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg
     2941 gttcgtatgg gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat
     3001 caagaagggg ctacggcatg gccactatta ccaaagagcg actgcaatgg ctggctaaca
     3061 tttctggccg cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta
     3121 tcgctctggc atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca
     3181 tgtgtattga cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc
     3241 tttatgctgc cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga
     3301 aggtgacgtt tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca
     3361 aagcggaagt tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa
     3421 caccgcttta cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat
     3481 tccatcacgc actgtcagat tcatcgcttg gctctggtga aatcgaagaa attaaaaccg
     3541 gtttgcgtgc tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa
     3601 tcgggccaaa cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg
     3661 ccatgcttaa gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata
     3721 tgccaggcag cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga
     3781 aatcgtggaa atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat
     3841 aagctgtagt gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg
     3901 gaatgtattc cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat
     3961 ggcttttgtg gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc
     4021 ggaaccaccg caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt
     4081 gcgatggcgg tggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc
     4141 ccggattgat ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc
     4201 tgttgttacc atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag
     4261 ataaattagc ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg
     4321 gtaagattgc tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca
     4381 gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc
     4441 aatct
//
LOCUS       DAJTXY010000207         4407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTXY010000207 DAJTXY010000000
VERSION     DAJTXY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.209"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..194)
                     /locus_tag="OO566_004027"
     CDS             complement(<1..194)
                     /locus_tag="OO566_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HCR6607079.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLA"
     gene            complement(205..2502)
                     /gene="xdhA"
                     /locus_tag="OO566_004028"
     CDS             complement(205..2502)
                     /gene="xdhA"
                     /locus_tag="OO566_004028"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6607080.1"
                     /translation="MEAREATATGESCMRVDSIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATHTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASTGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAFESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            2917..3672
                     /locus_tag="OO566_004029"
     CDS             2917..3672
                     /locus_tag="OO566_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6607081.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3751..3824
                     /locus_tag="OO566_004030"
     tRNA            3751..3824
                     /locus_tag="OO566_004030"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3783..3785,aa:Gly,seq:ccc)
     gene            3959..>4407
                     /locus_tag="OO566_004031"
     CDS             3959..>4407
                     /locus_tag="OO566_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6607082.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKG"
BASE COUNT         1113 a         1098 c         1128 g         1068 t
ORIGIN      
        1 tccgccagcg taattccccg cagctccgcc agattatgaa tatcaacaat atggcgataa
       61 cggtcattgt gatggtggag ctgaatcagt acgtcagtgc caccggcgag cagtttggcc
      121 tgcgggttgt cagccagcag gttgatggca tcggcaaggg ttgctgcgcg atggtaagaa
      181 gcaaaatcaa acatgatgtt atcctcaaat caatcctgcc agatggaact cttcatataa
      241 ccgttttggc gtcagcggca gtgtattgat tgcaacaccg gtagccatct tcaccgcgtt
      301 acgaatagca gcggcaacag gaattatggg tggctcaccc agtgacttat gtccgtatgc
      361 ggattgcggc tcattgattt cgacgaacgc gctttccagt tgtggcagat ccggcatggt
      421 cggcattttg taatccagca gattggggtt acggaccacg ccgcttttag catcgatgat
      481 catctcttca aatagcgccc agccaatgcc cattcccatt ccgccgtgta cctgaccttc
      541 tgccagcagt ggattaagaa tatgccctga atcatgaacg ttgaggatgc ggttgatggt
      601 gactttgcac agcgcaatat cgaccgtcag atcaacaaag gtacagccaa acgccggtgg
      661 gttagtggtg gttttgatgg agctttcagc agagagctgc ccgccgcgtt cagggtggta
      721 gaaagcgtcc atcgccaaat cttttaacga cattaacggc tcttccggtc gttcaaccag
      781 cacgatatgg ccttttatca gggtcaaatt catcgctgac tgatgtagca tgactgcggc
      841 gtgagcgatg attttctctt ttaataatag tgccgcactg cgcagcgcag gcgcggcaac
      901 atagctctgg cgtgaggcaa atgcgccggg atcgaacggc gtaacgtcgg tatcttgtgt
      961 tgaaataacg cgaacgtcgc tgaccggaac ccccacggtt tctgccacca tttgcgagaa
     1021 gacggtgtcg gcaccctgac cgatttccgt cgcgccgctt tgcacgttga tggttccatc
     1081 ctgattcatc agaaggcgcg cgcctgctat ttctacgccg acaggccagg tgttagaggt
     1141 gtagctaaaa caggcgacgc caacgccgcg gcgcaaattg ccttgctggt tctggcattc
     1201 tgcacggcgt ttttcccatt caaagatttt ccggcctttt tcaagacact ccggcaaccc
     1261 tgcgctgcaa atacgtttgc ccgtgagcgg attagcatct ccttcgcggg cggcgttgcg
     1321 taaacgaatt tcaacaggat caatacctaa cgctgtcgcg gcgtcatcaa gcatagactc
     1381 aaaggcaaat acgacttgtg gcgcgccata accacgcatc gcaccagccg aggggaggtt
     1441 ggtatagcag gtctttgaac tgtaagcgta ggcacaacga ggataaaggt aagcgacttt
     1501 attccccccc gtagaagcga tggagtgccc gtgagatgca taagcgccgg tgttagacag
     1561 aacatccaga ctataacctt tcaaggttcc gtcgcggttc acgcccattt gcccgtcaat
     1621 agtaaaagcg tggcgggtat gggttgcgag gaaacactct tcacggctaa gggaaacttt
     1681 caccggaatg ccgccaagct tgctggtcag gaatgccgcc attggctctt ccagtacatc
     1741 ctgtttatta ccaaaaccgc caccgacaaa cggtttgatg actcgtacgc atgaccaggg
     1801 aatatccagc gcctgaccaa ccacgcggcg aacaatgtgc gggatctggg tgctggaaac
     1861 gatggtaatt cgcgagtcat cctccatcca cgccagcgat gttacgcttt ccatgtgaca
     1921 atgttgaata acgggagtct gatagtgccc ctgtacctgg tagtcggcgg catcgattgt
     1981 ttgttggaca ttgcccgtcg acatcgtgct ttgtttcagt aaattgccgc cgttatggat
     2041 tggtgcagcg tcttctgtca gcgccgcttc tggcgtggtg ataacgggta attcttgcca
     2101 ctcaatgctg accaattgcg ccgctttttc tgccgtgagt tcatcgcggg ccacgacgat
     2161 ggcaacggcg tcaccatgat gacgaacatg gcgagttagc agtgcgcgat cggcggtatc
     2221 gcgcttgttt tcgtcaagtg tccaggcatg cccagctgta gcgaatggaa tatcaggcac
     2281 atcttcccaa gtaaaaatcg ccagtacgcc cggtaaactt ctggcttgtt catcattaat
     2341 acttacggca taaccatgtg cgatagggct acgtacatat ttcgcgtaac acatgcccgc
     2401 cataacataa tcgtcagtat atcgtgcccg cccggtgacc ttagcaatgg aatcgacgcg
     2461 catgcatgat tcacccgtag cggttgcttc ccgcgcttcc atagaaatcc cctcgatttg
     2521 aacatttggt tcttatggca aagatttagt ctaagcaggc aagatttacg ccagaaacga
     2581 cgataagcgt taggggagga aggaagtgtg atgacgttaa aaaaacacag atatgatacg
     2641 aaacaggact ctgaataggg acagtgatac gtatcaatat gagtggcgat aaaatcaatg
     2701 tgataaatca ggattattac gcatcaggat gggaaatagg gtaaaaagtc aggaacttcg
     2761 atacctctac agcgaatatt ttgctgatga tacttccctt tgccaaatga aattaacctc
     2821 tttctctatt accacgtttt tccagaagca agagattggg tatcaagagg ctggctgcta
     2881 tgatatggcg tcttgttttt taagcgagga aagattttga gtgcgggacg cctgaataaa
     2941 aaatctctgg gtatcgtgat gtttttatcg gttggactgc tattggcggg ctgttcgggt
     3001 agcaaatcat ccgatacagg aacgtattcc ggctccgttt acaccgtgaa acggggggat
     3061 acgctatatc gtatttcgcg caccacggga accagcgtaa aagagctggc gcgactgaac
     3121 ggcatttccc ccccttacac cattgaagtt ggtcagaaac taaaactggg tggggcgaaa
     3181 agtagcagta gtacacgtaa atcaaccgcc aaatcaacga ccaaaaccgc atcggttaca
     3241 ccgtcatcag cggtaccgaa atcttcctgg ccgccagtag ggcaacgttg ttggttatgg
     3301 ccaacgacag ggaaagttat catgccgtat tcgacagcag atggcggcaa taaagggatt
     3361 gatatctcag ctccacgggg tacacctatt tacgccgcgg gtgcaggaaa ggtggtgtat
     3421 gtgggcaacc agctgcgtgg ctacggtaat ctcatcatga ttaaacacag tgaagattac
     3481 attacggctt acgcccataa tgacacgatg ctggtaaata atgggcaaag cgtgaaggct
     3541 gggcaaaaaa tcgccactat ggggagcacg gatgcggcat ctgttcgcct gcatttccag
     3601 attcgttacc gtgcaacggc aattgatccg ctacgttact tgccgccgca gggcagcaag
     3661 ccaaaatgct gatggcgaat taatcagcag tcagcagcgc ggcacttgct aaggagagcg
     3721 taaggtttat aatgccttac gcatctcgaa gcgggcgtag tttaatggta gaacgagagc
     3781 ttcccaagct ctatacgagg gttcgattcc cttcgcccgc tccatgacac ttcttaaagt
     3841 tcaataactt actgaatcat aagggatatg gcttaacgcc ttccgatagt tttactcact
     3901 gtaaccaaca ctgtgactaa ctttgttgaa gtagtgagca gacctaaggt ctcagtacat
     3961 gctcaactca agaacctacc tttatcagcg taatggtgtc ttttacatcc gtcttaggat
     4021 gaagaccact ggtcgcctta ccgcttcgct tccttctcac aaccgttata aactagcgtc
     4081 agtatcttta cggaccaagg atagacgtac cgctatggct cactcccggc acatcaagtc
     4141 agcacttaaa gcaatccatg cagacaaccc taacgcctct tatgaggagc tacgggagca
     4201 cctgaagacc attgtcgagt gggaactcag tgtcagccgc gatgacctga acgaccctga
     4261 gtcctatcag ctttacgttg accagtacga cgatattaag tccaaccttc gggaagctgt
     4321 agcgactgag cacctgactg tagaccagca tcgctatatc aatgacgtta tcggtgtgct
     4381 taaagcctgt caggatagac tcaaagg
//
LOCUS       DAJTXY010000208         4380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTXY010000208 DAJTXY010000000
VERSION     DAJTXY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.210"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(64..822)
                     /locus_tag="OO566_004032"
     CDS             complement(64..822)
                     /locus_tag="OO566_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6607083.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO566_004033"
     CDS             complement(830..1933)
                     /locus_tag="OO566_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6607084.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3113)
                     /locus_tag="OO566_004034"
                     /pseudo
     CDS             complement(1943..3113)
                     /locus_tag="OO566_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3181..4206)
                     /locus_tag="OO566_004035"
     CDS             complement(3181..4206)
                     /locus_tag="OO566_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6607085.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1214 a         1148 c         1024 g          994 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 gaattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg caatattacg gaagatctca atataaatcg tggaaagctt tcgtagcagc
     2761 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     2821 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     2881 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     2941 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac
     3001 caaccaacaa tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc
     3061 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3121 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3181 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3241 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3301 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3361 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3421 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3481 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3541 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3601 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3661 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3721 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3781 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     3841 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     3901 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     3961 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4021 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4081 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4141 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4201 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4261 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4321 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg
//
LOCUS       DAJTXY010000209         4380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTXY010000209 DAJTXY010000000
VERSION     DAJTXY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.211"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..368)
                     /locus_tag="OO566_004036"
     CDS             complement(<1..368)
                     /locus_tag="OO566_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR6607086.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLG"
     gene            complement(483..558)
                     /locus_tag="OO566_004037"
     tRNA            complement(483..558)
                     /locus_tag="OO566_004037"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:Thr,seq:ggt)
     gene            complement(565..639)
                     /locus_tag="OO566_004038"
     tRNA            complement(565..639)
                     /locus_tag="OO566_004038"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(604..606),aa:Gly,seq:tcc)
     gene            complement(756..840)
                     /locus_tag="OO566_004039"
     tRNA            complement(756..840)
                     /locus_tag="OO566_004039"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(804..806),aa:Tyr,seq:gta)
     gene            complement(849..924)
                     /locus_tag="OO566_004040"
     tRNA            complement(849..924)
                     /locus_tag="OO566_004040"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(889..891),aa:Thr,seq:tgt)
     gene            1286..2236
                     /gene="coaA"
                     /locus_tag="OO566_004041"
                     /gene_synonym="panK"
     CDS             1286..2236
                     /gene="coaA"
                     /locus_tag="OO566_004041"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6607087.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2265..3230)
                     /gene="birA"
                     /locus_tag="OO566_004042"
     CDS             complement(2265..3230)
                     /gene="birA"
                     /locus_tag="OO566_004042"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6607088.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3227..4255)
                     /gene="murB"
                     /locus_tag="OO566_004043"
     CDS             complement(3227..4255)
                     /gene="murB"
                     /locus_tag="OO566_004043"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6607089.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1121 a         1095 c          990 g         1174 t
ORIGIN      
        1 cccagcagga tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg
       61 atcgcgccgt ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc
      121 gggcagtcta cgtgtgcgta gtgacgggtc ggggtgtcgt attcaacgtg agaagtgttg
      181 atggtgatac cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcgcgagca
      241 gcaccgccgt aggttttagc cagtacggta gtgattgcag cggtcagcgt tgttttacca
      301 tggtcaacgt ggccgatagt accgacgtta acgtgcggtt ttgtacgttc aaacttttct
      361 ttagacatcg attgtccctc taagacacgg ataaatcggt gatatcacca catcaaccag
      421 gcaacatgcc cgacttgttg aatgcaataa acagaaggaa aaaacaggga ggagaaaaga
      481 agtggtgctg ataggcagat tcgaactgcc gacctcaccc ttaccaaggg tacgctctac
      541 caactgagct atatcagcac atcttggagc gggcagcggg aatcgaaccc gcatcatcag
      601 cttggaaggc tgaggtaata gccattatac gatgcccgca tcctagaact cggctacctg
      661 attttcattc tgcactgaat atcgagagaa gctctcttta ttcgagccgg taagtgaact
      721 tatcgtctcg ggctacgcca tcgcgtggcc gaaattggtg gtgggggaag gattcgaacc
      781 ttcgaagtct gtgacggcag atttacagtc tgctcccttt ggccgctcgg gaaccccacc
      841 ggacttgatg gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg
      901 ctctacctac tgagctaagt cggcatcaag tagcgcgcat tctatggaga catgcgagtt
      961 catgcaacta aaaaattgca taatttgttt tattggtcac attttatgct acacgatgaa
     1021 gaaacagccg aacggagggc agattagcac actttttcaa catcattgtg ctcaacaatg
     1081 cgctcctgct aaaccataat tctttttatc agatggaata tctgtcacat tgcttttcaa
     1141 cgatagcttc ctggcagaga ttttttctta ttattcctcc ccatctggtg ttaccctcct
     1201 gcccattaac ccattcaaca gaactgtgac gcgccatggc aaatatcgct ttgccgatag
     1261 agctatgacc gccagaaaca tgcttatgag tataaaagag caaacgttaa tgacgcctta
     1321 cctacagttt gaccgcaacc agtgggcagc tctgcgtgat tccgtaccta tgacgttatc
     1381 ggaagatgag atcgcccgtc tcaaaggtat taatgaagat ctctcgttag aagaagttgc
     1441 cgagatctat ttacctttgt cacgtttgct gaacttctat ataagctcga atctgcgccg
     1501 tcaggcagtt ctggaacagt ttcttggtac caacgggcaa cgcattcctt acattatcag
     1561 tattgctggc agtgtcgcgg tggggaaaag tacaaccgcc cgtgtattgc aggcgctatt
     1621 aagccgttgg ccggaacatc gtcgtgttga actgatcact acagatggct tccttcaccc
     1681 taatcaggtt ctgaaagaac gtggtctgat gaagaagaaa ggcttcccgg aatcgtatga
     1741 tatgcatcgc ctggtgaagt ttgtttccga tctcaaatcc ggcgtgccaa acgttacagc
     1801 ccctgtttac tcgcatctta tttatgatgt gatcccggat ggagataaaa ctgttgttca
     1861 gcctgatatt ttaattcttg aagggttaaa tgtcttacag agcgggatgg attatccaca
     1921 cgatccacat catgtatttg tttccgattt tgtcgatttt tcgatatatg ttgatgcacc
     1981 ggaagactta cttcagacgt ggtatatcaa ccgttttctg aaattccgcg aaggggcttt
     2041 tacagacccg gattcctatt ttcataacta cgcgaaatta actaaagaag aagcgattaa
     2101 gactgccatg acattgtgga aagagatcaa ctggctgaac ttaaagcaaa atattctacc
     2161 tactcgtgag cgcgccagtt taatcctgac gaaaagtgct aatcatgtgg tagaagaggt
     2221 cagactacgc aaataatttg caggggagcg aatactcccc tttcttattt ttctgcacta
     2281 cgcagggata tttcaccgcc catccagggt tttattattc catcctgctc aagtaataaa
     2341 gccccctgtt tgtctattcc gcgtgaaatg ccaaatattt ctttatcacc aatgataagt
     2401 ttcactgggc gattaataaa attatccagc ttttcccagc gcgacagata aggtgccaat
     2461 ccttcttgtt cgaagagttc caacgcagca cgtaattcac gtattagcat ggccgccaac
     2521 gtattacgat caagattgat ccccgcttcc tgcagcgtga tccacccctg attaacgaca
     2581 ctctcttcaa cacggcgcat tgccatgttg atcccggatc caatgactat ttgcgccgca
     2641 tcgccagttt tgccagtcag ctccacaaga atgcctgcca gcttgcgatc ctgcagatag
     2701 aggtcattag gccatttgac acgaacttta tctgctccca gctttcgtaa tacttccgcc
     2761 atcacgatac cgataaccag acttaaacca atcgccgccg ccgggccttg ttccagacgc
     2821 cagaacatcg acaaatataa gtttgcgcca aaaggcgaaa accatttccg cccccggcga
     2881 ccacggccag cctgctggta ttctgcaaca caggcatcgc ccgatttaag ctctccgata
     2941 cgatcaagaa ggaactgatt cgtggagtca ataactggca gcacggctac actaccgcca
     3001 tccagctgac ccaatatctg ttcagcatta agtaactgga tgggctcagg caggctgtat
     3061 cctttacccg gaacggtaaa gacatcaacg ccccagtcac gcagtgtctg aatgtgttta
     3121 ttaatagccg cccggctcat tcccagcgtt tcacccaact gctcgccaga gtgaaattca
     3181 ccgttcgcta acagggcaat caatttcagt ggcacggtgt tatccttcat gaaattgtct
     3241 ccactgcgct cacttcacct gatgcaccaa taaagcggac ttcaggctca agccagacat
     3301 taaatttttc acccactttt tgtcttacat ggtgtgccag ttgcaccaca tcttcgcttt
     3361 ttgcattgtc ttcattaatg agaactaacg cctgttgacg gtgcaccgca gccccaccca
     3421 tttgcatccc ttttagctgg cactgatcaa taagccaacc tgctgccagt tttactgaac
     3481 cacccgcctg ggggtaattt ggtgctgttg gaaattgcgc cagtaatgct ttagccgttt
     3541 cggcagatac aacagggttt ttgaagaaac taccggcatt gccattcact tttggatcag
     3601 ggagtttggt ggtgcgcata tgacacaccg cattaaatac ttgctgtggc gttactgttg
     3661 taggatccag acgagttaag tcaccatacg ttagtacagg ttgccactct tttggcagac
     3721 gcagacctac ggctacaatg gcgaagcgat cctggtattc atgtttaaaa atactgtcgc
     3781 gatagccaaa acggcactct ttggcagtta agcgcacttg cttgcctgtc gccagttcaa
     3841 cacaatcaac ataagcgcaa actcgctgta attctacgcc ataagcacca atattctgga
     3901 taggtgatga gccgacacaa ccaggaatta atgccagatt ttccagacca ggcatacctt
     3961 cctgcaaagt gtattttacc aggcgatgcc agttttctcc ggctcctaca tgtaaatacc
     4021 acgcatcagg ttcatcatga atttcgatac ctttgatccg gttgatgatc accgtgccgc
     4081 gatagtcttc cagaaaaagt acattacttc cttcacccag aataagaacg ggttgtcctt
     4141 ctgcggttgc atgctgccag gcattgagta attgttgttc gtcttcggca catacaatgt
     4201 gctgagcatt atgatcaatg ccaaatgtgt tccagggttt taaggagtgg ttcatagctg
     4261 ctttcctgat gcaaaaacga ggctagttta ccgtatctgt ggggggatgg cttgtagata
     4321 tgacgacagg aagagtttgt agaaacgcaa aaaggccatc ctgacggata gcctttcgct
//
LOCUS       DAJTXY010000210         4351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTXY010000210 DAJTXY010000000
VERSION     DAJTXY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.212"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..518)
                     /locus_tag="OO566_004044"
     CDS             complement(<1..518)
                     /locus_tag="OO566_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607090.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTRGTLQQDALNICDNVVRTINSKMV
                     DVLESSKLLKTV"
     gene            complement(600..1091)
                     /locus_tag="OO566_004045"
     CDS             complement(600..1091)
                     /locus_tag="OO566_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607091.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRVEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(1412..2140)
                     /locus_tag="OO566_004046"
     CDS             complement(1412..2140)
                     /locus_tag="OO566_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6607092.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKMRGILIA
                     FTGTDQQKQEHLLKLAQSGKVEGI"
     gene            complement(3103..3756)
                     /locus_tag="OO566_004047"
     CDS             complement(3103..3756)
                     /locus_tag="OO566_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HCR6607093.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
     gene            complement(3749..>4351)
                     /locus_tag="OO566_004048"
     CDS             complement(3749..>4351)
                     /locus_tag="OO566_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6607094.1"
                     /translation="AKAYSQKNAGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFISLVEDRRKIEGDNAM
                     VFDGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVDRLVNVMAGVYKETGVNG"
BASE COUNT         1040 a         1100 c         1101 g         1110 t
ORIGIN      
        1 tctacggtct tcagcagctt agaggactcc agaacgtcaa ccatcttaga gttaatggta
       61 cggaccacgt tatcgcagat gttcagagca tcctgctgga gcgtcccccg agtgaaacca
      121 gtagaggact tctggtccca gagctttgtc tgatgagaca cgaggatagc ctgttcaatc
      181 ggctcaacgg tgatttcagt accagacgga gcggtcacga cttcaggtcg cttaccacca
      241 cggacaggaa cgaccacaga gcggtcagcc agagtgtgag caatgattgg cgtcggtaag
      301 ctgttacggg tcggcaggac ttctacaggg aacggaatgt tttcctgagc gtctttaagt
      361 acaccgttca gcatagcctt aaccgtctta gctaactgga gggcatagac ctgtgggtca
      421 gcgttagtag gcagaccttc attgacctga atgttgtcgg tgtcggtgat acgtactggc
      481 tcaataccga actggaaagc gtctttaatc gtcataataa tcaatctcct aaattagttt
      541 aaatactgtg agtcctcaaa actgaggagt ccctagcatc gggtcaggga ctgtgtgttt
      601 tacaggctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgggag
      661 cggccagcgg aatgggtagc actgcggagg ttttcaccat aggtccggcg ttcagctttg
      721 acctgttcgc ggcgctgtag ctctttctgc cattcgtctt cggtcatgaa catttcgcgg
      781 gtacgctctt tctgagacgc cagctcttta gcgatacgct cagcctcgac acgcttattc
      841 atctggcggc gttggtaggc ggtcatcagg tcattactga agaagttacc aatggtcacg
      901 ccagcttcag ccagagcttc cagtaactcc attgcctcgc ggctggtaga cggcaggcct
      961 gacaggtagc ccggaatgag ggtcttgata gcgagcgaca gcggataggg ctgaccgtcc
     1021 agactcacag tattctcgta gagattgatg tcggtgactg acagtttcca gtcttggtta
     1081 ttgaataaca tagtaattac tcctcttgag agttgagact atcggtcgac cacaggcgtg
     1141 ttcagtcctg cggcgtctcc ccgcatcggt catctcaaaa ggtttaaaat taggtattga
     1201 gtagacgcgc tactaacggt gtgctgacga cagctagcaa gctagcgaca gtttcatagc
     1261 gaccagttgg gctattagct cgtctcggga ttggtgctgg cgggtctcat cttccgggtc
     1321 tagaccaagc tctctgagga ggacttcagt ccggtagcca cggtaacgga gagtcatgcg
     1381 cccctcaggt gtgcgcgggt caatcatcat cttagattcc ctccacctta ccggattgtg
     1441 ccagcttcag gaggtgctct tgcttctgct ggtcggtgcc agtgaatgca ataaggatgc
     1501 ctcgcatctt gtcaaccatc tcaggcttac cgaagacctc cagcccctta gcgttgaacc
     1561 ggaagttata cccacctgct gagaacttct ggtctgacgg cttaatgata cgaaagcagt
     1621 cggaatactt cggatttaca gaccactcgt tactgattgg gttcttacga aagaggccac
     1681 gatgctggag aagtgcggtc attagcttct gagcgggcag accataggtt ttgcagtagt
     1741 cgccccaagt cacgcctcgg cggttagcgg actgaatggc tgctcgcttc ttctggtctt
     1801 ccagttgaag gactcgacgc tctaacgtct gaatatggtc gtaacacttg gcgcggaggt
     1861 gaacggaata gccagcgacc agaatgaaag tatgcttacg gtcgagacga tacagcggct
     1921 gctgtttccc ctgcgcgttt agatagacat cttcagtcac cccgatatga ctgctcagtt
     1981 ttgagcactg ctcagttttg agcgcgttaa gctcctcagc catattgcga atatcacgca
     2041 ggacatttcg gtgctccttg cctgtcagct cagcaatttc aactgaggtc atggagacaa
     2101 gctggtcgag gttaatgacg ctgatttctt gttgggacat ttacgtgtac tccttttgtt
     2161 tgcaccctga agtcgcaccc tactgaccta ctcaagcccc cacgtaaagg gagaccagca
     2221 ggcaacagga cgcaaacaga atgacttgag gtaattgtga tgtggtctgc gatgaccagt
     2281 agtgataaag gtgcaagaca gcatgtccga cgtccagatg gtgagcggcg atagacccaa
     2341 gtcaaaatga agacacgaga acgctaacgg gctgggagtc tgacagaggc tgtcttgcta
     2401 gccttggtat ggagaaagac ttaatgtcgg ggggcttccg cataacttga cgcatcaaga
     2461 ccagaggcgg aatgtatggc ccctatagtg atagctaagg gtccattccc tatagtgagg
     2521 gtataagatt tgctacctga taaggccata atctgacctc cagttagacc cgaagtatgg
     2581 agaccacatc atagcgacca tagtttactt cgggatgaca ggaggctgga cttaaagttc
     2641 ttcttattgg aattgtgata aacacaatcc ataacaagat aaactcatgg tctcctccag
     2701 cctgctggtc aggactcaag gttcaacctc agacaccact ggtctaacct ccttgagttt
     2761 ccgcctgtcc acaatggatg gcagacctca gcaatgatgt cttggttctt cacaatctcg
     2821 cacaccgatg gtctcataga cggaagttcc gctgaggtct gatgttctcc gcctgtcaaa
     2881 ggagagagat tgctacgtta aagggaatgg tctcaggcca aggtgattta acactaggcg
     2941 tctgcggatg gtttccaatc ggtccgctac cggaagcctg agacttaatg aatgtgtgtg
     3001 ggtcgtgaat gttattaagg tggggactta gggtctctcc cctatagtga tagttaagcc
     3061 cccttcccta tagtgagggt atattcaatc tgacctaaag ctttactcgc tagctgcctg
     3121 ctgctgttcc ctcgccttcc tgtcccggtc ctcaatgccc ttgagcgccc tttccggcat
     3181 gctgttaaac atggcttcga cttctttcgg tctggtaatc tgctcagtga caataatgtt
     3241 cagcagctta aaaagaccat gcgctaactg agggtcgtca tcaatattaa tttcacccgg
     3301 atggaccgcc tgattgccaa cgatacgaca aatatcagca gctcgctgga ccaatggaga
     3361 caatcctttg gctacaagcg acttaatgtc cgtgtcgata tgcttccccg gctcaccgag
     3421 atggaccatc agcttctgaa gacttaagcg caggagtgcg gcagcgcctt taggagaaag
     3481 gttaacaatg cttctggcct ccatgtagtc ggacttacag tcgtctggca tgtctgggtt
     3541 aggcatctca acgtttgctg acgctggtat aatcatggtt tcgtcatacc aataagcttt
     3601 ctcattgcat cgttcacatt tagaaaaggc gaaaggtgtc tcataaatcc cgttagcgcc
     3661 atattttaag cggacccagc gctgttgaga ataagcgcca caattcgggc agttaaaagc
     3721 agtgccacca aactctgccg gataatactc agccatttac tccagtctcc ttataaacac
     3781 ccgccatcac attaaccaat ctatctacct cgacctcttt cgcgtaacgg tcgtaagtta
     3841 tagacccaga gctatgaccc ataatcgcct gagcatacgc caattcgacc ccatgttgtt
     3901 ttagctgggt cgcaactgta tgtctaaacg agtgaaaagc taagccgcta cggtcagcaa
     3961 ggactttagg tagcaacgtt cggttaaacc atttgctcac ctgctcaccg tagccgttct
     4021 tcatcagctt gaggccatcg aataccatag cattatcacc ctcaatcttt ctacggtctt
     4081 ctacgagtga tataaagtca gccaatacaa aaccataagc accatcgacc agcgggacac
     4141 agcggtcact atgggcgttc ttgatactct tacccggcag gctgctatcg tcttcgttga
     4201 tatggataaa cacggtcccg gcctcagtgg tcctgacatc tttgacctga agttgggcca
     4261 cctcgttaag tcttgctcca gtgattgcag ccagagcggt gacgtaataa tggtatggct
     4321 tacctgcgtt cttctgagag tacgctttag c
//
LOCUS       DAJTXY010000211         4341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTXY010000211 DAJTXY010000000
VERSION     DAJTXY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.213"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            149..742
                     /gene="tetC"
                     /locus_tag="OO566_004049"
     CDS             149..742
                     /gene="tetC"
                     /locus_tag="OO566_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6607095.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(855..2060)
                     /gene="tet(B)"
                     /locus_tag="OO566_004050"
     CDS             complement(855..2060)
                     /gene="tet(B)"
                     /locus_tag="OO566_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6607096.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            2142..2765
                     /gene="tetR(B)"
                     /locus_tag="OO566_004051"
     CDS             2142..2765
                     /gene="tetR(B)"
                     /locus_tag="OO566_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6607097.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2743..3429)
                     /locus_tag="OO566_004052"
     CDS             complement(2743..3429)
                     /locus_tag="OO566_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6607098.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(3437..3823)
                     /locus_tag="OO566_004053"
     CDS             complement(3437..3823)
                     /locus_tag="OO566_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR6607099.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            complement(3816..4079)
                     /locus_tag="OO566_004054"
     CDS             complement(3816..4079)
                     /locus_tag="OO566_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6607100.1"
                     /translation="MSWSHYRPLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVL
                     QWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            complement(4076..>4341)
                     /locus_tag="OO566_004055"
     CDS             complement(4076..>4341)
                     /locus_tag="OO566_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6607101.1"
                     /translation="ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQR
                     IERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1396 a          938 c          796 g         1211 t
ORIGIN      
        1 catatcttaa ttggatgtta ttagctttat tttgatagcg agaatgctgt tcaatataca
       61 taaaataacc taaatgttct taagattgtc acgaccacat catcatgata ccataaacat
      121 actgacggta tgttatttta aatctatcat ggaaaataaa aatcatcaac aagaaaattt
      181 taagagtacc tatcaatcac tggttaactc agcacgaata ttgtttgttg aaaaaggcta
      241 tcaagctgtt tcaatagatg agatctcggg aaaagcgttg gtgaccaaag gtgcctttta
      301 tcatcacttt aaaaataaaa aacaattact cagtgcctgt tataagcagc aattaattat
      361 gattgatgcc tacatcacaa caaaaactga tttaacaaat ggttggtctg ccttagaaag
      421 tatatttgaa cattatcttg attatattat tgataataat aaaaacctta tccctatcca
      481 agaagtgatg cctatcattg gttggaatga acttgaaaaa attagccttg aatacattac
      541 tggtaaggta aacgccattg tcagcaaatt gatccaagag aaccaactta aagcttatga
      601 tgatgatgtg cttaaaaact tactcaatgg ctggtttatg catatcgcaa tacatgcgaa
      661 aaacctaaaa gagcttgccg ataaaaaagg ccaatttatt gctatttacc gcggcttttt
      721 attgagcttg aaagataaat aaaatagata ggttttattt gaagctaaat cttctttatc
      781 gtaaaaaatg ccctcttggg ttatcaagag ggtcattata tttcgcggaa taacatcatt
      841 tggtgacgaa ataactaagc acttgtctcc tgtttactcc cctgagcttg aggggttaac
      901 atgaaggtca tcgatagcag gataataata cagtaaaacg ctaaaccaat aatccaaatc
      961 cagccatccc aaattggtag tgaatgatta taaataacag caaacagtaa tgggccaata
     1021 acaccggttg cattggtaag gctcaccaat aatccctgta aagcaccttg ctgatgactc
     1081 tttgtttgga tagacatcac tccctgtaat gcaggtaaag cgatcccacc accagccaat
     1141 aaaattaaaa cagggaaaac taaccaacct tcagatataa acgctaaaaa ggcaaatgca
     1201 ctactatctg caataaatcc gagcagtact gccgtttttt cgccccattt agtggctatt
     1261 cttcctgcca caaaggcttg gaatactgag tgtaaaagac caagacccgc taatgaaaag
     1321 ccaaccatca tgctattcca tccaaaacga ttttcggtaa atagcaccca caccgttgcg
     1381 ggaatttggc ctatcaattg cgctgaaaaa taaataatca acaaaatggg catcgtttta
     1441 aataaagtga tgtataccga attcgattgc gtctcaaccc ctacttcggt atctgtatta
     1501 tcacgtgtat ttttggtttc acggaaccaa aacataacca caaggaaagc gacaatattt
     1561 agcaacgcag cgataaaaaa gggactatgc ggtgaaatct ctcctgcaaa accaccaata
     1621 ataggccccg ctattaaacc aagcccaaaa cttgccccta accaaccgaa ccacttcacg
     1681 cgttgagaag ctgaggtggt atcggcaatg accgatgccg cgacagcccc agtagctcct
     1741 gtgatccctg aaagcaaacg gcctaaatac agcatccaaa gcgcacttga aaaagccagc
     1801 aataagtaat ccagcgatgc gcctattaat gacaacaaca gcactgggcg ccgaccaaat
     1861 cggtcagaca tttttccaag ccaaggagca aagataacct gcattaacgc ataaagtgca
     1921 agcaatacgc caaagtggtt agcgatatct tccgaagcaa taaattcacg taataacgtt
     1981 ggcaagactg gcatgataag gccaatcccc atggcatcga gtaacgtaat taccaatgcg
     2041 atctttgtcg aactattcat ttcacttttc tctatcactg atagggagtg gtaaaataac
     2101 tctatcaatg atagagtgtc aacaaaaatt aggaattaat gatgtctaga ttagataaaa
     2161 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
     2221 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa
     2281 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
     2341 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta
     2401 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag
     2461 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
     2521 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg
     2581 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc
     2641 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct
     2701 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt
     2761 cttaaaagca gcataacctt tttccgtgat ggtaacttca cggtaaccaa gatgtcgagt
     2821 taaccaccct ttagattcat aaagcgaaaa taatgcggct ccaacgtacc cacctaaatg
     2881 gaaacggcgt tcactccaat ctaaacacgc acaacagatt ttacgtgaat gtttggaagg
     2941 aacgtcaatt cccatttcat gaaaatattg aataccactt aatgtgatca ttgaaccatt
     3001 ttcagtgatc cattgctgtt gacaaaggga atcatagatc ttaacggcaa cttcgccagc
     3061 taaatgatca tagcaagtac gtgcttttcg taaatgcact ggcgtggaaa ctttggcatg
     3121 tacgccatgg tttaaggaga tccccatcat actttccatc aattcagcaa tatcttttcc
     3181 tgctagccga aaataacgat gcttgccttg agctactact gtgattagct ggcaatctaa
     3241 taatttagat aaatgactgc tcgccgttga agctgatata ttcgccacag aacttagctc
     3301 agtggccgtc caagctcgcc catccatcaa agcactgagt attttaactc gtgaaatgtc
     3361 agacatagcc gcccctatcg cggctattga ggactcaaag gtaacctctt ttcgtattaa
     3421 attagccatc gcaagttcac tttattgccc aagggagcgt aacagatgca gccatactat
     3481 cattgtccgt tattaatatc agttggttag catggtcact gtattgcact aaaatattaa
     3541 tgttattctc cgccaatact cgtgctattt cgccaagttc ccccggtttt tcctgtttta
     3601 acttacgaat taatggtgtc cggatcgcaa gtactaacag tccagcttgc tctagcgcta
     3661 ttttagcttc ctttccttgt tcaacaagaa aatgagcatg gcattcatca ccaaccgtaa
     3721 atatccctcc cccttccaat ccaatacctt tattacctaa tgtttttcct agtaatgcta
     3781 attgtcctat ttgattatct aaaacaacgt gaacatcaaa cattatcctt gtccttttta
     3841 aatgaatatt cgcgagtaat atgagcaata ctaattttgt aaaaatcaaa tattgactct
     3901 cgcccttctt gttgagcttt cgcatgtaaa acatgatttt tccattgcag aactgcgtat
     3961 tcgttttccc accaagatag cgataacatt tttccttctg tagctagact ttgaaaacgt
     4021 tcaattgaaa taaaaccagc tacatgactt aatagtggtc ggtaatgact ccaacttatt
     4081 gatagtgttt tatgttcaga taatgcccga tgactttgtc atgcagctcc accgattttg
     4141 agaacgacag cgacttccgt cccagccgtg ccaggtgctg cctcagattc aggttatgcc
     4201 gctcaattcg ctgcgtatat cgcttgctga ttacgtgcag ctttcccttc aggcgggatt
     4261 catacagcgg ccagccatcc gtcatccata tcaccacgtc aaagggtgac agcaggctca
     4321 taagacgccc cagcgtcgcc a
//
LOCUS       DAJTXY010000212         4339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTXY010000212 DAJTXY010000000
VERSION     DAJTXY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.214"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..324
                     /locus_tag="OO566_004056"
     CDS             <1..324
                     /locus_tag="OO566_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR6607102.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="OO566_004057"
     CDS             383..586
                     /locus_tag="OO566_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCR6607103.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            586..1611
                     /locus_tag="OO566_004058"
     CDS             586..1611
                     /locus_tag="OO566_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6607104.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(1664..1753)
                     /locus_tag="OO566_004059"
     tRNA            complement(1664..1753)
                     /locus_tag="OO566_004059"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1717..1719),aa:Ser,seq:cga)
     gene            1847..2644
                     /gene="mtfA"
                     /locus_tag="OO566_004060"
     CDS             1847..2644
                     /gene="mtfA"
                     /locus_tag="OO566_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6607105.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2745..2820
                     /locus_tag="OO566_004061"
     tRNA            2745..2820
                     /locus_tag="OO566_004061"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2778..2780,aa:Asn,seq:gtt)
     gene            2982..4244
                     /locus_tag="OO566_004062"
     CDS             2982..4244
                     /locus_tag="OO566_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6607106.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1211 a          999 c         1036 g         1093 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gatctattta cctgcaataa
      541 aactttacca ccacgcgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagaaaat attccaagta attccaagtg
     1621 tgatttttgt cactgactta atgatgtata agtgattgaa ttttggcgga gagaggggga
     1681 tttgaacccc cggtggagtt gcccccactc cggttttcga gaccggtccg ttcagccgct
     1741 ccggcatctc tccgctcaga tggttgccat gatgccagga aatttggcat tttaacagtc
     1801 cctgtccgtg caattttgtt caagtgacga gtttgcgagc aaaacgatga ttaagtggcc
     1861 ctggaaagta caagaatcag cacatcaaac tgcccttccc tggcaggaag cactatcgat
     1921 ccccctttta acgtgtctga cagaacagga acaaagcaaa ttggtcgctc ttgccgaacg
     1981 ttttttacag caaaaacggc ttgttccttt acagggcttt gagctgaatt cattaagaag
     2041 ctgccggata gcacttctat tttgcctgcc cgttctggag ttaggactgg aatggctgga
     2101 tggttttcat gaagtcttaa tttatcctgc gccatttgtg gtcgatgatg aatgggaaga
     2161 cgatatcggt ctggtgcata accaacgtat tgttcagtca ggtcagagct ggcagcaagg
     2221 gcctatcgtt ttgaactggt tggatataca agattctttt gatgcttctg gttttaacct
     2281 gattattcat gaagtcgctc ataagctgga cacccgtaac ggcgatcgcg ccagcggagt
     2341 tccctttatt tcgttgcgtg aggttgctgg ctgggaacac gatcttcatg ctgcaatgaa
     2401 caacattcag gaagaaatcg aattagttgg tgagaatgcg gcgagcattg atgcttatgc
     2461 tgccagtgat cctgctgaat gttttgccgt actttctgaa tatttcttta gcgccccaga
     2521 actttttgct cctcgtttcc cttcattgtg gcaacgtttc tgtcaatttt atcaacaaga
     2581 tcctttgcag agactgcatc acgctaatga tacagactcg ttttcggcga cgaatgttca
     2641 ttaattaaca actttgcaga ttaattaacc aattgaaatg gcttatgaaa tttagtgttg
     2701 acagacaagg taccgctaag taatatgcgc cccgttctca cgattcctct gtagttcagt
     2761 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     2821 attttctgtt ttcatgcatc cttgcgaatc tttatctgat gttgattcaa caggttagtg
     2881 tgaaaactct tctcggtgca ttttgatttt accctacgca tcgggaaaaa ttggtggtca
     2941 aatctgggat caggttagtt cgataatgga gtgaccccca tatatccctt accgacgcaa
     3001 aaatccgcac cctcaagcct tctgataaac cctttaaagt ctccgattct cacggtctgt
     3061 atctgctggt caagccgggt ggctcccgcc actggtatct caaataccgt attagcggta
     3121 aagaatcccg cattgcgctg ggtgcctatc cagccatctc cctgtctgat gcgcgacagc
     3181 aacgtgaagg tatccgtaaa atgctggcgc tgaatatcaa cccggtacag cagcgggctg
     3241 ctgaacgtgg ctcacgaaca ccggagaaag tttttaaaaa cgtggcgctg gcgtggcata
     3301 aaagtaacag gaaatggtcg cagaacaccg ccgaccgtct gcttgccagc ctgaacaatc
     3361 acatctttcc ggtcatcggg aacctacctg tatcagaact taaaccccgt catttcattg
     3421 acctgctgaa agggatcgag gaaaaaggtc tgctggaggt tgcgtcccgc acacggcagc
     3481 acctgagtaa cataatgcgc catgcggtcc atctggagtt aatcgatacg aaccctgcag
     3541 caaaccttgg cggcgtgacc acacctcctg tcagacggca ctatcctgcc ctgccgctgg
     3601 agcggctgcc tgaactgctt gaacgtattg gggcatatca tcagggccgt gaactgaccc
     3661 ggcatgccgt tctgctgatg ctgcatgtgt tcattcgctc cagtgaactg cgtttcgccc
     3721 gctggtcaga gattgatttc acaaaccgag tctggacgat acccgcgacg cgagaaccca
     3781 ttattggcgt gcgttattcc ggccgcgggg caaaaatgcg aatgccgcat atcgtccccc
     3841 tctcagaaca gtccatcgcc attctgaaac agattaagga tatcaccggt aataatgaac
     3901 tgatcttccc cggcgaccat aacccgtata agccaatgtg tgaaaacacg gtcaataagg
     3961 cactgcgggt gatgggttac gacacgaaaa aggatatctg cggtcacggc ttccgggcaa
     4021 tggcatgcag tgcgctgatg gaatcgggtt tatgggcaaa ggacgcagta gaacgccaga
     4081 tgagtcatca ggagcgcaat accgtgcgca tggcttatat tcataaggca gagcacctgg
     4141 aagcccgcaa agcgatgatg cagtggtggt cggattatct ggaagcatgc cgagaatctt
     4201 atgcaccgcc ttatacaatt ggtaaaaata agtttatccc atagtatact aacgaatccc
     4261 acactctggc aaattaccac cacctctaca ggaggtggtt ctatatagac ttttatcaac
     4321 tttatgacga aaaacacct
//
LOCUS       DAJTXY010000213         4270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTXY010000213 DAJTXY010000000
VERSION     DAJTXY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.215"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            278..808
                     /locus_tag="OO566_004063"
     CDS             278..808
                     /locus_tag="OO566_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6607107.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELISN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWCQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            815..1048
                     /locus_tag="OO566_004064"
     CDS             815..1048
                     /locus_tag="OO566_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6607108.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1628..2746)
                     /locus_tag="OO566_004065"
     CDS             complement(1628..2746)
                     /locus_tag="OO566_004065"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6607109.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSNKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2864..2953)
                     /locus_tag="OO566_004066"
     CDS             complement(2864..2953)
                     /locus_tag="OO566_004066"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607110.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(3389..3550)
                     /locus_tag="OO566_004067"
     CDS             complement(3389..3550)
                     /locus_tag="OO566_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6607111.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
     gene            complement(4202..>4270)
                     /locus_tag="OO566_004068"
     CDS             complement(4202..>4270)
                     /locus_tag="OO566_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6607112.1"
                     /translation="HDHADELYRSCLARFGDDGENL"
BASE COUNT         1231 a          874 c         1038 g         1127 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcaggacgct gacactccct gataatctgc cggaaacgaa acaggcgatt gctgagggcg
      121 ttacacgcgg tttaccatgc cggagagata aaaggccgtc aggatgtgat tcaggcgctc
      181 acagaggcag ggattgacgt ggtcagggtg acgcgaagca gtatcagcat tgcagacccg
      241 aacggcggga agaatgtcag actgaaagga gcgttttatg agcaatcttt tacagacggg
      301 tgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa tgctgaacga
      361 cgagttcagg aagctcgaag aatctgtaaa cagggaactg atctcaaacg aatctttaat
      421 caggaacgct atcaacgacc atacatcagc cctgaaagaa cttctggaga ggtatcagaa
      481 aacgaccgtc gatacgatgg acgcacactg gaaaaccgta ctgaaaatga gcgtgaaacg
      541 ctggctgtgg ctgattatcg tctcagtact gatgtttgcc acgaccggga gcctgctgtg
      601 gtgccagggc atgaagataa acgcgaacat gaatattctc cgcgagcaga aagagagtct
      661 ggagaagctg aacgcgaaga catggggcgt gcgttatcac gaggacagca acggacgttt
      721 tctggtgttg cccgagggga tgaaagccga aacgaactgg acgatggaaa acgggaaaca
      781 gaatgccgta aggctggtac aggagtaaca gagcatgacg gagctggaaa gacatttgct
      841 gaacgcatta gagcaactgc aacaggacta tatgcagcgg ctgaacgaat gggagagcgc
      901 cttcgtggaa ttgcagaaaa tgttttcgct tacgcaacgg gacaacgcga tgctgaacga
      961 gcgggtcatg cagttgagtc agcaggtgca gcacttgagc gagcagacag aacgcttgag
     1021 ccagttatac agcgagaaca ggagataagg gacgaacggc tgatacagga atgcgaacat
     1081 gcgttatccc tggagcgtga gcgacagccg gaaatacagg aacggacact ggatggccct
     1141 tcgctgggat ggtaatcaga gacaggctaa aatgttgtca gcgcgtttcc ggtgcatttt
     1201 ttgggataat ggttggtttg gggggcaccg ggaacgcgct tcagtcagac cttgctgaaa
     1261 acataggaac gcgttaaaac gcatacgggc gcgattctgg tgtgtctttt gttgctgtgt
     1321 tttgcctcta ttgccagatc gcgctcattc ccctgaactt ctgttttccc gctcaggtcc
     1381 ccgtcttctt cgcttccgaa agcatgaaaa aaatccgttt cagctgtctc tggctggcaa
     1441 ttcctgcgtg attcacatgg ctgcatagct atgcatgtgg ttaaaattta ccgggtgcga
     1501 tcgcggcagt ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gtgatcgcgc
     1561 cgaacgcgtt tcagcggtgc gtacaatgag atgttatggt aaattcaagt cttagatttt
     1621 atgatgttta attagctttt tttgcgttta taatgttcat cttttttatt aaaattgatt
     1681 caatctttga tgtgggtatt aatattttat ttgacaaata gaaagttaaa aaaaacacca
     1741 ttaatagtat aaataaatac ccaaaaacag aagcgttatt aatttcaatt attgatgtta
     1801 atctgtatat tactacatgc tgaatcaaca tggatatgta gatgcattta gcatatgagt
     1861 tgaaaaaata gaggttaaat ttcttattaa ttattaatgc tgctttgtaa atgataatgc
     1921 ttatgcacat taaaggtatt attgataact ggtgattgac tatgtttgtt ttgaactcat
     1981 taggccctat taataatact gtcaatatac atataacaac cgatagaata actgtggtgt
     2041 ttttttgtaa tttatttttg tatttaaatg caagcatccc aaccataaac tcaggaagtc
     2101 ggcaagcaaa aaaccaaaat gggaagttta aaaatgatga tgagatattt gatatcggga
     2161 atgttgtaat tgttatcaca aataaaaaaa atatgttacg tttaaatgta aaggtattca
     2221 taacgtgaaa aactatcggt gatatcacgt acagcataat aacgcaccca ataaaccact
     2281 cgcctgtaag gaaaagaaaa tcataagaga aatgggcgtt taatatacca tcaagggcaa
     2341 aaaatactgg aattattcta gtgtactggc ctcctgttgt cattattgat acgagaggga
     2401 ctgtattaat tatctcttta tttaaataag acaataaaat taagagtaaa gagataaaaa
     2461 agtaagtgat aatataagga gtaaggatcc ttactccttt gtcaaggaaa taatcaatgg
     2521 tattctttct atctagtgaa ataaatgcca aatatccaga tataaaaaaa aacaagctca
     2581 cacctacctg gccaagaacc cctccataaa atgatggtat gaaagatagc ctggtttggc
     2641 taaatatgta gccataatga gatataataa caaaaatgca ggctgttact cgcaatgtat
     2701 caattgacgg atctctatta gacataacct ttatttcgct gttcaaatta tcgcctttag
     2761 ttgatgatgt aaaattaacg ctataaataa gctacacatt ctagagcagc gaaaggatga
     2821 ttcaagtatt tttaataata aaacgcattg ataatgttta tgttcagatg gtgctgatag
     2881 ctttgtcatg ctgttccact gatgttgata acgacagaga cttttgtcct tttcttgtta
     2941 gatgctgcct cagattcgga ttatggcgtt aaaggggttt gacgctcaac cgaacggttt
     3001 cacacgttaa gagctttttg tctgataaat caaaatataa tggacattga aaaacgccct
     3061 caaaacggtg tctttttaat cgaagattgg cacaaaacgg acaccaccgt ccattagtcg
     3121 attttttaga cggcttttct cgcctaacgt gagttttcgt tcggttgagc atcagacccc
     3181 ttttatattg tagatttcta tttttagaca gttggaacag gacaattaac aacagatcta
     3241 cttgagatca tttgcttgtc gtcgtagtct ctttcccaga aacgaaaaaa ccgccctgca
     3301 aggcggtttc tcgaaggttc tcagagcgac caactctttg aaccaaggta actggcttat
     3361 gaggagcgca gtaaccaaat ctgtcctttc agtttagcct taacaggcgc ataacttcaa
     3421 gactaactcc tctaaatccg ttaccaatgg ctaccgccac tggcgatttg tcgtgtcttt
     3481 ccgggttgga ctcaagttga tagttaccgg aacgggcgtg gtagtcggac tgaacggggg
     3541 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
     3601 gaatgagata aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
     3661 ggagagcgca cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg
     3721 tttcgccacc tctgacttga gcgtcggttt ttgtgatgct cgtcaggggg gcggagccta
     3781 tggaaaaacg ccatcggcgc ggccttcttc ctgtttctgg tcttttgctc acatgttctt
     3841 tccggcgtta tcccctgatt ctgtggataa cagcatttcg tttttgagtg agtagatacc
     3901 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc
     3961 ctggacgtgc attttctcct tacgcatctg tgcggcattt cacacccggt atggcgcact
     4021 tttcatacaa tctgcactga tgccgcatgg ttaagcccgt atacactccg ctatcgctac
     4081 gtgactgggt catggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg
     4141 cttgtctgct cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatat
     4201 gtcagaggtt ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg
     4261 cgtggtcgtg
//
LOCUS       DAJTXY010000214         4265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTXY010000214 DAJTXY010000000
VERSION     DAJTXY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.216"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            150..819
                     /locus_tag="OO566_004069"
                     /pseudo
     CDS             150..819
                     /locus_tag="OO566_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1062..1757)
                     /locus_tag="OO566_004070"
     CDS             complement(1062..1757)
                     /locus_tag="OO566_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6607113.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1750..3177)
                     /locus_tag="OO566_004071"
     CDS             complement(1750..3177)
                     /locus_tag="OO566_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6607114.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3188..3907)
                     /locus_tag="OO566_004072"
     CDS             complement(3188..3907)
                     /locus_tag="OO566_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6607115.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1143 a         1098 c          879 g         1145 t
ORIGIN      
        1 ggggaccgaa tcgttatata aaacactgag gtaactctca tgcttcatat ccagtattca
       61 tgatgctggt tttttgctga tatttaaccc gaacattcct gatacagact attaatacaa
      121 cattatcttc gacagatttt agatatatta tgagtgaaca aatcaaacag gatatcgatc
      181 tgattgagat tttatttcat ctgaagaaaa agattcgtgt tatccttttt attatggcta
      241 tctgtatggc aatggtgctg ttgtttctgt atatcaataa agacaataca aaagtgattt
      301 acagccttaa aataaaccag acaacgccag gtatacttgt tagctgtgat agcaataata
      361 attttgcctg tcagaccaca atgactgaag atgttattca gcgaattact acattttttc
      421 agaccagccc agatatcaag aacagagaaa taaggctgga atggtcagga gataagagag
      481 atttaccaac tgctgaagag gaaatatctc gcgtgcaggc gtctattatc aaatggtatg
      541 cgtcagaata ccataatggc aggcaagttc tcgatgagat acaaacgcct tcagcaatta
      601 acagtgagct ttatacaaaa atgatatacc tgaccaggaa ctggtcattg tatccgaacg
      661 gtgatggctg tgtaactata agctcaccag aaataaaaaa taaataccct gctgccattt
      721 gcctagctct gggatttttt tctaagcatt gtaatttctg taatgttttg ccttgtcaaa
      781 aaaatggtag atgaatacca acaaaactct gggcagtaat aaacagcatt ctgatagaga
      841 gtgcctgtat aaagctgaag tgacaatgac cgggaaggga acccgttttc aggaatggat
      901 gcctgtcttt ttcactgtaa atatcctggc attactaatg cctggatatt tacagaagag
      961 cagcataacc atgacggaac ggggttaagt caggttgtcc ttaacgctat tttcaacctc
     1021 gtatgccttc ttcaggttta tgttcagatc tcatatctct ctcagcaatc ctcaataatc
     1081 agatacaccg ctttcaccgg accatgaacc ccgacgactt tgataagctc aatatccgcc
     1141 gttgagctgg gaccactgat gatgttaata catgacggca ttcgttcacc ggcctgcgct
     1201 ttctgatgca atttttctgc gagttgcgct acacgcggca ggattgtgct tttacgcagg
     1261 ataaaaagag aagattccgg gagcaggctc aatgaacgcc cgcgctcggc ggcggaaaaa
     1321 agaaccacgc ctcccgattc ggttaaacca tattcagcat acacgacacc cactttagcc
     1381 tgctctgcct gcgagatatt ctcggcacct ttcgccggat cccaaacaac ggcattgcat
     1441 tcctgctgca aacgttcact aatccccaat tcctccagcc tcgtgtcacc gctaatcacg
     1501 accgactgat ctcccagctc tttacacaga cgtattgcag cttctgccgc cttcgcctcg
     1561 ctggtcagct cacagcgcgt caacataaca tcgctggcaa actgaataaa cgcgtcacag
     1621 cgctgctgtt ggttaagttg ggtaagccgc tcgttagcat agttgttaag cggcgcatct
     1681 tctgcttgcg gttcaagtcg cagcgggcga cccagtgcct gagcaacgtt attcaaaaat
     1741 tcgctccgat tatccattct ttttctcctg cgcctgatgt ttcttaaacc aactacggaa
     1801 actctctccg tcagcttcag gaagatcgcg tgcttccatc cagtcgctaa tcgcgccaaa
     1861 tttgagtggt gttttaccgc cattgataaa ccagcttgcc gcatgagcgc cggccatcat
     1921 cccgactttc cacaatcctg gatgactatt ggcataagcg aacattttta tcgcccgttg
     1981 ctctgctttt gcggtgatcc ctttttcagc catcacccga cgatgacgca aaatcagttt
     2041 tgacagcgga atacgcaccg gacacacgct gtcacaagca gtgcataaag agcaggcgta
     2101 gggtaaatct ttaaaatctt tatagccgcc aagtagcgga gaaatcaccg caccaattgg
     2161 ccctggataa atagagccat atccatgacc gccaatatgg cgatatgccg gacaagtatt
     2221 catacaagcc ccgcagcgaa tacagcgcag cacatcccga aattcagagg ccagcacctc
     2281 agaacgcccg ttatcgacaa taaccagatg aaactcttca ggaccatcaa cgtgccccgc
     2341 ttcgcgcggc cctgtcagcc aggtgttgta tcccgtcaaa cgtgcaccaa cggcactgcg
     2401 cgccagcatg gtgatcaata catctacctc ggcaaacgtg ggggcaatac gctccattcc
     2461 catcactgca atatgcgttt taggcagcgt ggtacacatt cgcgcattac cttcattggt
     2521 caccaggcat accgaaccgg tctctgccac cgcgaaatta cagccggtaa tacctatttc
     2581 agcactgagg aaatcttcac ggattttttg ccggatgaat aaggtcatcg cttcaggcgt
     2641 ttccggcccc tcatagccca gacgttcgtg tagcactcga cggatctgat ggcgatcttt
     2701 atgaattgcc gggaccacaa catgagatgg cggatcttga tccagctgga gaatatattc
     2761 acccagatcg gtttcaatca cctgaatgcc agcatcctgc aacacatgat tgacaccaat
     2821 ctcttcggtc accatcgatt tagatttcac caccttccgg gcatttttgc gttgggcaac
     2881 ctgtaaaatg tagcgggtag cgtcttcttt ggtttttgca aaatagacgt gaccgccgtt
     2941 ttgcgtcact ttttctgaga gctggtacag ataagcgtcg agattactca aaacatgatc
     3001 acgtatctgg gcggcccgat cgcgccactc ctcccagtgc cccaattcat cgaccatttt
     3061 ttgccgattt gccccaatac gctgctgcgc gtttgccacc gctttgcgca tgatcggatc
     3121 ttcaatttgc tgacggatgc gtgtcttaaa atctgtatta ctggttttga tcgacatctt
     3181 tatatcctca gcggctcatc aacacttcag caatatgcat cactttgact ttctgccctt
     3241 cccgttgtaa tcgcccactg atgttcagca ggcaactcac gtcagcacca attaaatact
     3301 cagggcggac ttccatcagg tgcacaacct tttctttcac catctcgccg gatatttcgg
     3361 ccattttgac cgagaacgtg ccgccaaatc cgcagcaggt atcctgttca gcaaaggtca
     3421 acagctccag tccacgcaca tttttcagca gcgtaagtgg ctcgtccttc actcccagct
     3481 tacgggccag gctacaagat gggtgataca cagctctccc ttgcaaactg gcacctacat
     3541 cgactacccc taatttatta acaataaaag aggtgagatc ctgcatacgc gcggcaacct
     3601 tttcggcacg tgatgcccat tcaggttcat ccgccagata cgtcgggtaa ctttttacgg
     3661 cataggtgca agagccagcc ggtgaaataa tgggatcgtc gttatcctcc agtgcggcga
     3721 tcagattttt catccctgga atcgcttctt tgatataacc gctattgatc gcaggctgac
     3781 cgcagcatcc ctgtttctcc gggaaattta cgcgacagcc gagtttttcc agtagcagca
     3841 cggagtctcg tgccattctt gatttcaggg cgtcaccaat acaggtgaca aagaaattga
     3901 cattcacaac taatactcca ttacttcatg cccatttatg cccgatgttt cccccccacc
     3961 gggggatcaa tcatcaataa atatggcgtt ctttttaaat tggctagcaa cataccaccc
     4021 ctcatttgta tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     4081 tttctttcgg catcgctcac attttatggg gaaatcgacg atcatttttg gcataagaaa
     4141 tccatttaaa aatggctaca tcccttatac ctattcagag ctacttattc aggggttatt
     4201 gagatataca ctgatggtgt acaaaaaaat caccgacaat aatcacgtat caagtaatag
     4261 tcgga
//
LOCUS       DAJTXY010000215         4187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTXY010000215 DAJTXY010000000
VERSION     DAJTXY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.217"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..148)
                     /locus_tag="OO566_004073"
     CDS             complement(<1..148)
                     /locus_tag="OO566_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR6607116.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGL"
     gene            complement(298..459)
                     /locus_tag="OO566_004074"
     CDS             complement(298..459)
                     /locus_tag="OO566_004074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607117.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(456..644)
                     /locus_tag="OO566_004075"
     CDS             complement(456..644)
                     /locus_tag="OO566_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6607118.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            904..1239
                     /gene="iraM"
                     /locus_tag="OO566_004076"
     CDS             904..1239
                     /gene="iraM"
                     /locus_tag="OO566_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6607119.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1520..1651)
                     /locus_tag="OO566_004077"
     CDS             complement(1520..1651)
                     /locus_tag="OO566_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR6607120.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2151..2227)
                     /locus_tag="OO566_004078"
     tRNA            complement(2151..2227)
                     /locus_tag="OO566_004078"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2191..2193),aa:Arg,seq:tct)
     gene            complement(2329..2404)
                     /locus_tag="OO566_004079"
     tRNA            complement(2329..2404)
                     /locus_tag="OO566_004079"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2369..2371),aa:Ile,seq:cat)
     gene            complement(2548..3369)
                     /locus_tag="OO566_004080"
     CDS             complement(2548..3369)
                     /locus_tag="OO566_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6607121.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3384..3740)
                     /locus_tag="OO566_004081"
     CDS             complement(3384..3740)
                     /locus_tag="OO566_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6607122.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3753..>4187)
                     /locus_tag="OO566_004082"
     CDS             complement(3753..>4187)
                     /locus_tag="OO566_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6607123.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPES
                     PESFMKRPKRRSWVNEKYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTL
                     PLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1155 a         1133 c          823 g         1076 t
ORIGIN      
        1 gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac ggggaaaacc
       61 tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg tatgacacac
      121 ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga agctgacaac
      181 aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag cgtctcagac
      241 gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc tcacttttta
      301 tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc catacgttgc
      361 atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac tgtcttttat
      421 ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt tgcaccactg
      481 accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt tatggccacc
      541 ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc cgcaagatct
      601 gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct ttccactatc
      661 tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa aaccatacct
      721 taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac gagatataaa
      781 ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc tctatggttt
      841 aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa acaaggaaac
      901 taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat atctttttca
      961 gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt cttacctcct
     1021 gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt gctacctgta
     1081 accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa aaacagccag
     1141 gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg ttgcactctg
     1201 caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc acatctgaca
     1261 aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg tgccattgca
     1321 tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga ttatcccata
     1381 tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt tcttttaaca
     1441 aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt aaaaatttct
     1501 cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac gggaaagcaa
     1561 caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc cggctgctgc
     1621 aatgccattc acaaaaacag taagcaatca ctttttaccg taacaggtga taatccagat
     1681 atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc tggctattct
     1741 gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact catcgcaaac
     1801 cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac gatcaggagt
     1861 acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga ctatatcaat
     1921 acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac ccacaccata
     1981 tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat actcaatcaa
     2041 tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata tcctgagcca
     2101 ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata tggcgcgctg
     2161 tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc ggctgagcta
     2221 acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca ccaacaaccc
     2281 tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg gtggcccttg
     2341 ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac tgagctaaag
     2401 ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaac ccgctcagcg
     2461 gcgggttgta gaaactcttc taacgtcagg cataaaacgc ccatcattat gacgaattta
     2521 ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact gccgctcagc
     2581 ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct taactgtttt
     2641 ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa atgcctccgt
     2701 tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga taacaggcat
     2761 accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc aatccttaca
     2821 ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta ccacctccct
     2881 gacttttttc cattcttccc agtaagacgg atacacacct ttcgtacact ttgcccatac
     2941 cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt caataaattt
     3001 ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat aatcctcaaa
     3061 agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa gttttctcaa
     3121 cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg ttgcccgcgc
     3181 cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca tctcagcccg
     3241 attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat cttttgacgt
     3301 tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg gtaaatcttc
     3361 cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac ccccagacgt
     3421 ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc atcgtccatt
     3481 aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc caggtcgcga
     3541 cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga caggcttaat
     3601 ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt tccctcctcc
     3661 gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg cgggtaaggt
     3721 aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt tgcaaaggcg
     3781 tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc gaacgccaga
     3841 ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa aatatcgtga
     3901 gattttgtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc gtcggctggc
     3961 ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc gttaacccag
     4021 ctgcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac ggcaatgctg
     4081 accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag cggcgcaaga
     4141 ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccgg
//
LOCUS       DAJTXY010000216         4103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTXY010000216 DAJTXY010000000
VERSION     DAJTXY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.218"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(179..709)
                     /gene="cybB"
                     /locus_tag="OO566_004083"
     CDS             complement(179..709)
                     /gene="cybB"
                     /locus_tag="OO566_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6607124.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            898..1899
                     /gene="gap"
                     /locus_tag="OO566_004084"
     CDS             898..1899
                     /gene="gap"
                     /locus_tag="OO566_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6607125.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDNTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVMQLIRTLEKFAKL"
     gene            complement(1941..3380)
                     /gene="aldA"
                     /locus_tag="OO566_004085"
     CDS             complement(1941..3380)
                     /gene="aldA"
                     /locus_tag="OO566_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6607126.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3577..>4103)
                     /locus_tag="OO566_004086"
     CDS             complement(3577..>4103)
                     /locus_tag="OO566_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6607127.1"
                     /translation="TTGRAEATIQVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
BASE COUNT         1080 a         1031 c          950 g         1042 t
ORIGIN      
        1 cgttgatgag atcaaagatc gggtgaattg attaaaaata agccagatgg acgtatgggc
       61 aaggattatg gttttatttg tcatacaaat aaatatgata ggcgcttccg atgtagaccc
      121 gtattcttcg cctgtaccac gggtcggttt tagtacaggc gttttcttta aatatccttc
      181 aggaacgttt acgcggcatc atgcgtagaa gtgtgttgtc cttccagaaa tagtggtgtg
      241 ccagtgcagc cgcagcgtgc aacccgatga caaaatatcc cagattcgcc agcgtctcat
      301 gccacgactt taagctatca acccgttcga aattggcctc tgaagcgtaa ggcatcgtca
      361 aaccaaacgc aaaccacggg ttgccccggt tatacatcat caccaaacca atcactggca
      421 gcgcaataaa caggagataa atcaccaaat gtcccaaatg cgccagtccc gtcatcatcg
      481 gtttcggctt aggtataatc ggcggggttg ggtatttcag tcttaacaaa agacgaacca
      541 ccatcagcac gagaattgag atgccacagg aaacatgaat catgttgatg agcggccgat
      601 cgctacgtgg gaagaaacca cgaaactcca ttgcgcaata cgctgcgata accagcaaaa
      661 agaccagcca gtgaatgctg atttgtaacc ttgagtattt attttccata acatttcctg
      721 ctttaacata attttccgtt aacataacgg gcttttctca aaatttcatt aaatattgtt
      781 cacccgtttt cagtacgtta cgtttcactg taagaatgga gattgcccat cccacaatcc
      841 tggtctaagc ctggaaagga tcaattttca tccgaacgtt cctgacagga gaaaaccatg
      901 agtaaagttg gtattaacgg ttttggtcgt atcggtcgac tggtgttgcg tcgtttactt
      961 gaagtcaaaa gcaacataga cgttgtcgct attaatgatc ttacttcccc aaaaattctc
     1021 gcctatctgc tgaaacatga ttcaaactat ggtccgttcc cctggagcgt tgattttacg
     1081 gaagattcac ttatcgttga tgggaaaagt atcgcggttt acgccgaaaa agaggctaaa
     1141 aatattccgt ggaaagcgaa aggggcagaa atcattgtcg aatgtactgg cttttatacc
     1201 tccgccgata aatcgcaggc gcatcttgat gctggtgcga agaaggtgtt gatttccgct
     1261 cctgccggtg aaatgaaaac catcgtttat aacgttaatg acaacactct ggatggcaac
     1321 gacaccattg tttccgtggc gtcatgcacc actaactgcc ttgcgccgat ggccaaagcc
     1381 ttgcatgaca gtttcgggat agaagtcggc acgatgacga ccattcatgc ctatactggc
     1441 acccagtcac tggtggatgg cccgcgtggt aaagatttac gtgcttcacg cgcagcggca
     1501 gaaaatatca ttccccacac tacgggggcg gcaaaagcca ttggtctggt gatcccggaa
     1561 ctgagcggca aactgaaagg tcatgcgcaa cgcgtgccgg tgaaaacagg ttcggtcact
     1621 gagctggtgt ccattctcgg aaaaaaagtg actgccgaag aggtgaataa cgcacttaaa
     1681 caagcaacca ccaataacga gtcatttggt tataccgatg aagaaatagt ctcttccgat
     1741 atcattggca gccatttcgg ttcagtgttt gatgccacgc aaacggaaat taccgccgtg
     1801 ggcgatttac aactggtgaa aacggtcgcc tggtacgata acgaatatgg cttcgtcatg
     1861 cagcttattc gcaccctcga aaaattcgct aaactctgac gcgcacaggc ggaggaaaaa
     1921 acctccgcct ctttcactca ttaagactgt aaataaacca cctgggtctg cagatattca
     1981 tgcaggccat gtttaccatc tgcgccgcca ataccggatt tacgccatcc ggcgtggaaa
     2041 ccttgcatag cttcgaagtt ttcacggttg atgtaagttt caccaaactt cagcccttta
     2101 atggctttca tcgcgacgtt cagattttgg gtatagattg atgaggtcag gccgtaatca
     2161 ctgtcattag ccattgagat agcatcttcc agcgtgtcaa atgcgacaac tggcagcacc
     2221 gggccaaagg tttcctcatg cataatcgac atttcctggc gaacatccag cagcaatgtc
     2281 ggcggataat aatatccttt cccctctacc gctttgccac ccaacgccac tctcgcccct
     2341 tcttctactg cgcgcgccac tttttgctca accctttcca gcgccgcggc gttaatcaac
     2401 ggccccatcg caatgtcgtt gcgttcagcg gggttaccaa attgaaccgc ctgcaacgct
     2461 tcacccagcc gattgacgaa ctgatcataa atgcctttct gtacataaac acgttctgca
     2521 cagttacaca cttgcccact attaatgacg cgtgaatcaa cgatggcttt gactgccagt
     2581 tcaagatcgg catcgtccat tacgatagcc ggtgctttac cacccaattc cagacacact
     2641 ttggtgatgt ttttcgccgc agtcgccatg atcttctcac ctgcagagac gctgcctgtc
     2701 atactgacca ttgcgacctt tgggttaccc gccagttctt gcccaacggt ttcaccacgc
     2761 cccagtacaa ggttaaacac gccgcgcgga aggcctattt catcgacgat tttggcgaat
     2821 gcaatcgcat tgtttggcgt aaattcactg ggtttaatga cgatggtatt accggtcaaa
     2881 agagcgggag ccattttgcg ggcaatgagg aagaacggaa agttccacgg cagaatgccg
     2941 gtagtcacac caagcgcacg tttaaacaga agaatatttt ctcctggacg atcgctttga
     3001 ataatctcgc cctcgtaacg ccgtgcccac tccgccatgt aatcgatata gtcggcagta
     3061 aaagccactt cgacttcagc tagctgctgg atcttgcccc cttcttcaac aatcagcgca
     3121 ctgatttcac tggcgcgttc gcggatcccg gcggagattt tgcacaacca actggcgcgt
     3181 tcaatagcag gcaacgcttc ccattctggt tgtgcacgtt ctgctgcatc gattgcctta
     3241 cgggcatcct cggcctgacc atcgggtatg cgggaaatga cagcctctgt agcagggttt
     3301 accacatcaa tccatgcgtc tccacgccag gtaacaaact gtccatcgat atacatagga
     3361 tgttgaacgg gtactgacat gggcgactcc tgtgatttat atgtttgttt tcggtgaata
     3421 tattgttaat taacatcaat catcagagag acggcatcgg gaaaggacaa ttctgtggag
     3481 ggcttcataa aaacaagtgt aaaaggaata aaaggcagaa ataaaagtca ggagtcatgc
     3541 ccgtttgcaa acaagtggtt cacaaacggg caatttttaa cgtaacgagc gactttccat
     3601 cgcctcgatg agcaccgcat catgtttcag cgtttgccat gcatggatga cttctgccgg
     3661 aaaatcaacg tgaacgataa aatctcgccc gcggggaccg tagccatcgc tatcatcgtg
     3721 taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc ataatccttg
     3781 taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa cgaatccggg
     3841 ataacttaac cagcgtggtg catcgggatt gtccccggtc atacggcgga acgttgccat
     3901 cgtgcgccgc tgcatggtgg ggtcctgtac aacgatagcc gtatgaactc gttctacggc
     3961 ctgattcaat agcgtgatgc taaagcgtgc gttttcaccg cagtttgttg actggtcttc
     4021 aatccagatt ttttcatgcg gaatatgcca gaactgatga gcgatatcca cctggatggt
     4081 cgcttctgct ctgccagtgg tgc
//
LOCUS       DAJTXY010000217         4049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTXY010000217 DAJTXY010000000
VERSION     DAJTXY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.219"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..203)
                     /locus_tag="OO566_004087"
     CDS             complement(<1..203)
                     /locus_tag="OO566_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6607128.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALK"
     gene            513..2285
                     /gene="aspS"
                     /locus_tag="OO566_004088"
     CDS             513..2285
                     /gene="aspS"
                     /locus_tag="OO566_004088"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6607129.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2403..2855
                     /gene="nudB"
                     /locus_tag="OO566_004089"
     CDS             2403..2855
                     /gene="nudB"
                     /locus_tag="OO566_004089"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6607130.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2884..3624
                     /locus_tag="OO566_004090"
     CDS             2884..3624
                     /locus_tag="OO566_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6607131.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3659..>4049
                     /gene="ruvC"
                     /locus_tag="OO566_004091"
     CDS             3659..>4049
                     /gene="ruvC"
                     /locus_tag="OO566_004091"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6607132.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLL"
BASE COUNT          998 a          998 c         1141 g          912 t
ORIGIN      
        1 tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa cacgggctga
       61 ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc atcggcagta
      121 tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac caacgcagtg
      181 gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg cctcacacct
      241 taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag ttcgggcaac
      301 acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt ggtaacgagg
      361 cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa tagccgcctt
      421 ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg cagcaggggg
      481 catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt ggacagctcc
      541 gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt cgtcgtgatc
      601 ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag gtatttttcg
      661 atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag ttctgcattc
      721 aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat atggcgaccg
      781 gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat gttctgccgc
      841 ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac ctcgacctgc
      901 gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc ctggtgcgcc
      961 gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc aaagccacgc
     1021 cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa ttctacgcac
     1081 tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt gaccgttact
     1141 atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag cctgaattta
     1201 ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa gtgatggaag
     1261 cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat ttcccggtaa
     1321 tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg cgtaacccga
     1381 tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta tttgcggggc
     1441 cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc gcatcgctga
     1501 cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg aaaggtctgg
     1561 cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc ccggtagcga
     1621 agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg caagatggcg
     1681 atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg ggcgcactgc
     1741 tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca ccgctgtggg
     1801 ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca atgcaccatc
     1861 cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg gaaaatgcgg
     1921 tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt tcagtacgta
     1981 tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac gaagaggaac
     2041 agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg ccgcacgcag
     2101 gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac aatatccgtg
     2161 acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa gcaccgagct
     2221 ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag gctgagaata
     2281 actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca actgagtgaa
     2341 ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa cgaagaggca
     2401 gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc tatgcacaag
     2461 atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg cagtcggtaa
     2521 ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa gtaaaggaag
     2581 aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag cgcacggtag
     2641 agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg cgtaatacgg
     2701 aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact gaacatctgg
     2761 cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg agcaaccggc
     2821 aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc tggagatatt
     2881 tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc gcaggatgct
     2941 aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc taagctgggc
     3001 ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc actgtctaac
     3061 aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg tgatgatgat
     3121 gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc aatcatgatt
     3181 gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc attcagcaaa
     3241 tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa aaaaggcgtg
     3301 atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga agcaggtgct
     3361 gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg ggaagaaatg
     3421 ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc ggaagtttcc
     3481 atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact gatgcgtctg
     3541 atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg tgaaatctct
     3601 gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga gacgcgtgat
     3661 ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg tcatccgcca
     3721 ggtaggtagg caactgtctt acctgggtag cggatgcata cgcaccaaag tggatgattt
     3781 accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc agttccagcc
     3841 tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag ccctgaaact
     3901 gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc cggtatttga
     3961 atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg aaaaaagcca
     4021 ggtgcagcat atggtccgca ccttgctga
//
LOCUS       DAJTXY010000218         3879 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTXY010000218 DAJTXY010000000
VERSION     DAJTXY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.220"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..175)
                     /locus_tag="OO566_004092"
     CDS             complement(<1..175)
                     /locus_tag="OO566_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6607133.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQ"
     gene            complement(386..1783)
                     /gene="ccp"
                     /locus_tag="OO566_004093"
     CDS             complement(386..1783)
                     /gene="ccp"
                     /locus_tag="OO566_004093"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6607134.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2188..3837
                     /locus_tag="OO566_004094"
     CDS             2188..3837
                     /locus_tag="OO566_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6607135.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDLYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVSREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1012 a          979 c          903 g          985 t
ORIGIN      
        1 tctgacgagc gttgccatca agatataatt catcattgat aatctggaat gcgacatcat
       61 cgcgcatttc gtgcagcgga aatcgttttg actccgcgat agtagaaatg gcctttgcgc
      121 caaaacgtga atcgagtagt tctgagcgga aatccgttaa cagcttctgg tccatttcga
      181 actccttaaa tttatttgaa ggcaataaaa aagtaggatt tatccgcaat ggaagcaagg
      241 cattacaggc ttaatttaaa taacaaaatc ctaagcagaa aaacgacttt ttataatata
      301 aacatttaac atgataatat taaaaacaga ctttattatc gtaataaaaa tcgcccaaac
      361 attcctgttc gggcgatcaa attaattatt gtttatcctg catatacggc gtgtacaccc
      421 cgttcagact gtgcaggaaa gctacgatat catccacatc ctcctgcggc agctctttgc
      481 ctacctgata gcgcagcatc agtttcaccg ccccgtccag cgtcggcacg tcaccgcgat
      541 ggaagtacgg tgccgttaaa gcaacgttac gtaaaccggg tactttctga cgcaatttat
      601 cacgctcttc tttagtcaca ttcatacgac caatatccgc cgccgtaatt tccccaaagt
      661 taaagtcttt tttcagcacc aacggttcaa aggaacgtcc gccgagaata ataccaccat
      721 gacaagttgc acatttatta tctttaaata attgatagcc ttttttctgt tgcgccgtca
      781 gcgcattttc atctccacgc aaccatttat caaatgggga atccggcgta attaatgttt
      841 tctcaaattc agcaatggca tcagtaatat tttcgccact gaaaccttgc ggataaactt
      901 cgaggaactg cgctttaagc tgcggatctt tttccagctt agcaataatt tcgtcccagg
      961 atttcgacgc catttcaatc gggttcaacg gcggtccacc tgcctgatcc tgcaatgttg
     1021 ccgcacgacc atcccagaac tgctcaacgt taaatactga gttaaatacc gtcggcgcat
     1081 taatcggccc aactgcgcca ccaacaccaa tcgatgtttt tctgccatcg acgccccccg
     1141 cattcaacgt atggcaatga gcgcatgaaa tggtgctatc agccgataaa cggggatcgt
     1201 gatacagcgc aaagcccaac gccacttttt gcgcatcggt aggcagtttt tgcgggatgg
     1261 gctgcaccgg ttcattgcga tgctccggag cagtatcatt gctggcgtaa tattccgcgc
     1321 gctgttttgc aatccaggcc agtatttccg cccgctcttc atcactcacc ttacccgccc
     1381 agtgtagcgc ggtataacgc gttggtggca tagtttcata ctgcatcacc cattcaatct
     1441 tattcagatc gctttgcgaa acgggtttat cagccagcag tgccgcacgc acggcctcga
     1501 ggttaaaaga tttatatcca agcttaatgt cgtaatccat caactgtttc gcgccaggaa
     1561 tataataata ggcgggtaat tctgccgaag gcgtgtggca atagtcgcat cctttttcgc
     1621 ggagaaagcc taaaacctta ttattttcac tgacagcaga tgcctgaaca tcggcctgtt
     1681 tactgcgttt attatcgtgg taccacacat aaccagataa ccctaaatag caaatcgcga
     1741 cgccagccag gccgatcgcg gtaatacgtg agaccatttt cattattctt ccctcacggt
     1801 ggtgagttat tgtgacccag aaaattttcc agaatgctgc aaacgtctct gcgagacaaa
     1861 gccatcataa aaaacaggga gatacgcttt ttgataggaa tcaatcaatt acatgaatat
     1921 aaactatcaa taagatagcc tgtgcctatt acccagatca ggaatggcat atcatgattg
     1981 cggcgagagc aggattgaat gttctgaaaa tgaaaaacaa caccaaatcc gtatttttat
     2041 aaattccatt gatattagtg cgttatattt tttgacaagt taataaacgt gatccaccgc
     2101 acgctttgtc gcccaccagg cggagcgaat gactaccctt aaagaaaagc ccgataatta
     2161 gcgacgaatt tcggaggttg gatccttatg ctcaatcaga aaattcaaaa ccctaatcca
     2221 gacgaactga tgatcgaggt cgatctctgc tatgaactgg acctgtatga attaaaactg
     2281 gatgagatga tcgaggcaga accggaaccc gagatgattg aagggctgcc cgcctctgat
     2341 gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc atgttcagtc ggcgaaaatt
     2401 ttccccgaca gtaaaacctt tcccgactgc gcacccaaaa tggacccgct ggatatttta
     2461 atccgctacc gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt tgttgaaaat
     2521 cacttctggc tgccggaggt ctactccagc gagtatgtat cggacccgca aaattccctg
     2581 aaagagcata tcgaccagct gtggccggtg ctaacccgcg aaccacagga tcacattccg
     2641 tggtcttctc tgctggcgct accgcagtca tatattgtcc cgggcggccg ttttagcgaa
     2701 acctactatt gggattccta tttcaccatg ctgggactgg cggaaagtgg tcgggaagat
     2761 ttgctgaaat gcatggccga taacttcgcc tggatgatcg aaaactacgg tcacatcccc
     2821 aacggcaacc gcacctatta tttgagccgc tcgcaaccac cggtttttgc gctgatggtg
     2881 gagttgtttg aagaagatgg tgtacgcggt gcgcgccgct atctcgacca ccttaaaatg
     2941 gaatatgcct tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc ctatcgccat
     3001 gttgtgcgga tgccggacgg atcgctgctc aaccgttact gggacgatcg cgacacgccg
     3061 cgtgacgaat cctggcttga ggacgttgaa accgcgaaac attctggtcg cccgcccaac
     3121 gaggtgtacc gcgatttacg cgcgggagcg gcctccggtt gggattactc ttcccgttgg
     3181 ctgcgtgata ctggtcgtct ggcgagcatt cgtaccaccc agttcatccc catcgatctg
     3241 aatgccttcc tgtttaaact ggagagcgcc atcgccaaca tctcggcgct gaaaggcgag
     3301 aaagagacag aagcgctgtt ccgccagaag gccagtgccc gtcgcgatgc ggtaaaccgt
     3361 tacctctggg atgatgaaaa cggcatctac cgcgattacg actggcgacg cgaacaactg
     3421 gcgctgtttt ccgctgccgc cattgtgccg ctctatgtcg gcatggcgaa ccatgaacag
     3481 gccgatcgtc tggcaaacgc cgtacgcagc cggttactga cacctggcgg gattttggca
     3541 agcgagtacg aaaccggtga acagtgggat aaacccaacg gctgggcacc gttacaatgg
     3601 atggcaattc agggatttaa aatgtatggc gatgaccttc tgggtgatga aatcgcgcgc
     3661 agttggctga aaacggtgaa tcagttctat ctggaacagc acaaaatgat cgagaaatac
     3721 catattgccg atggtgtttc ccgcgaaggc ggcggtggcg agtatccgtt gcaggatggg
     3781 tttggctgga ctaacggtgt ggtacgccgt ttaattggtt tgtacggcga accataatat
     3841 ttttacagcc agccgctaac ttcctgctgg ctgtaaaat
//
LOCUS       DAJTXY010000219         3739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTXY010000219 DAJTXY010000000
VERSION     DAJTXY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.223"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            109..2106
                     /gene="betT"
                     /locus_tag="OO566_004095"
     CDS             109..2106
                     /gene="betT"
                     /locus_tag="OO566_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR6607136.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2169..3446
                     /locus_tag="OO566_004096"
     CDS             2169..3446
                     /locus_tag="OO566_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR6607137.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          905 a          852 c          941 g         1041 t
ORIGIN      
        1 attccaccat gcattacgat taacgccgca tagccagttg aactttgcta ctttgtgaga
       61 ggtagtacct tctatccagt gcgaatttaa ttaatggaat aaatgattat gagtgaaaat
      121 gatacaatcc caaagaagtc tacaagtcag attaacaaag cggtattctt tacatctgct
      181 ttgctaattt tccttcttgt cgcctttgcc gccgtattcc cggatgtcgc cgacaaaaat
      241 tttaaactac ttcagcaaca aatcttcacg aatgccagct ggttctacat ccttgctgtg
      301 gccctgattt tactgagtgt cacgttcctt ggactctcac gctacggtga tatcaagctg
      361 ggcccggacc atgcgcagcc tgatttcagc taccactcct ggtttgcgat gcttttttcg
      421 gcagggatgg ggatcggcct gatgttcttt ggcgttgccg aacctgtaat gcattatctt
      481 tcgccacccg ttggcactcc agaaaccgtt gcggcagcta aggaagcaat gcgtctgacc
      541 tttttccact ggggactgca cgcatgggca atttatgcca ttgtggcgct gattctggcg
      601 ttcttcagtt accgtcacgg tctgccttta actctgcgct ccgcactcta tcccattatt
      661 ggcgatcgca tatacggacc tgtaggacat gcggttgata ttttcgctgt tataggcacg
      721 gtctttggcg ttgcgacatc actgggttac ggtgttttgc aggtgaatgc cggtttgaac
      781 catcttttcg gggtgcccat caatgaaacg gtgcaggtaa ttctgatcgt ggtcatcacg
      841 gggttagcga cgatttcagt ggtgtccggt ctggataagg gaatacgtat cctgtctgaa
      901 ctcaatctgg gtctggcttt gttgctcctg gcgctggtcc tgtgtctggg accaaccgtg
      961 cttctgctga agtcatttgt ggaaaatacg ggcggttatc tttcggaact ggtgagtaaa
     1021 acgttcaacc tttacgcgta tgagcccaag tcgagcaact ggctgggggg ctggacatta
     1081 ctgtactggg gatggtggct ttcatggtcg ccgtttgtgg ggatgttcat cgcacgggtc
     1141 tcccgcgggc gaaccattcg cgagtttgtc accggcgtgc tgtttgttcc cgcgggtttt
     1201 acgctaatgt ggatgacggt gtttggtaac agcgcgatct atctcattat gaaccagggg
     1261 gccacagacc tcgccaatac tgttcagcag gatgtggcgc tggccctgtt taatttcctg
     1321 gagcatttcc cgttctcttc tgtgctgtca ttcattgcaa tggcgatggt catcgtcttc
     1381 tttgtaacgt ctgctgattc gggggcaatg gttgtggata ctctggcatc aggtggagtg
     1441 gcaaacacac ccgtctggca gcgaatattc tgggcctcgc tcatgggcat tgttgcaatt
     1501 gcgcttctcc ttgccggagg gctaagtgcg ctgcaaacgg tgacaatagc gagtgcattg
     1561 cccttctcag tgatcttact aatatctata tacggacttt taaaagcttt gcgccgggat
     1621 ttgaccaagc gtgaaagcct gagcatggcg acaattgctc ctacggctgc acgtaaccca
     1681 attccttggc agagaaggtt acgcaatatc gcgtatctgc cgaagcgatc tcttgtgaaa
     1741 cgttttatgg acgacgttat ccagcctgcc atgacgctgg ttcaggagga actgaacaag
     1801 caggggacga taagccacat tagtgatgca gtcgacgatc gtattcgtct tgaagtcgat
     1861 ttgggcaacg agctgaattt tatatatgaa gtgaggcttc gcgggtatat ctcaccgacc
     1921 ttcgcgctcg ccgcaatgga taatgatgag cagcagactg aacaacatcg atattatcgc
     1981 gctgaggttt atctcaaaga aggcggtcaa aattatgatg tgatgggctg gaaccaggaa
     2041 cagctgatta atgacatact ggaccagtac gaaaaacacc tgcacttcct gcacctggtt
     2101 cgttaatagc aacatgccgt cctgggggcg gcaattatta tcccggccgc aatatgaggg
     2161 aatgcagaat gatttcacgc tggaaatgga tgctgaagca gacaattaaa aaactatggt
     2221 tcagggcaac gttattcgca attgtcgcga taataacggc ccttttatca attcttttta
     2281 aatcaatgat acctgagtcg gtttccgtga aggttggtgc ggaagcagtc gataacattc
     2341 tgaacatact ggcatcgagt atgctggcag tgaccacatt ttcgctgagt atcatggtca
     2401 cagcctacgg ttcagccact actaatgtga ctcccagagc tacgcgttta gttgttgaag
     2461 acgtcaccac acaaaatgta ctggccacct tcatcggttc ttttctcttc agtctggtag
     2521 ggattattgc cctcaatatg ggagcttatg gagaaagggg gagagtcatt ttattcattg
     2581 tcacactggt tgtcattgcc ttaatcctca tcacattgct tcgctggata cagcatttga
     2641 cctctctggg gagggttggt gagacaacgg cacaagtaga acaggcggcc atcgaaacat
     2701 ttattgcgag agcaagaaat ccctgtctcg gtggatatcc atggcttgag aacaatgaac
     2761 agccgaaagg aacggttgca gtttatccga agaagattgg ctatgttgaa tatattgata
     2821 tggtgaaact tagcaagctg ctgaccaatg atccccgtca tgtatacctc gtggcgcagc
     2881 caggcagttt catacatccg tccatgccag ttttgtacct gagtcagggc caggagtcgt
     2941 caatcaacac cgatttactt gagacgatta ttgtctcgga tgcacgttca tttgctcagg
     3001 atcctcgatt ttgtcttagc gtcatggccg aaatagcctg ccgggccctt tcccctgcag
     3061 tgaacgatcc tggaaccgcc attgatgtca ttggcagagg tgttcgtata ctttccactt
     3121 acgcgcagaa taaatctgat gaaatagaag tgaaatatcc ttcagtacat gttgcaccac
     3181 ttcagaataa cgatctactg gaagactttt tctcacctgt cgcgcgcgat ggtgccagta
     3241 tgagggagat tcagataaga gtccttaaag gactgtcgat gctgagtaaa ggttggcctg
     3301 agatatttgc tgaagccgca cataccctgg catttgaaac attagagcat gcaactcgtg
     3361 ctgaccatat agattctgat agatatctaa taaaatcaac ttattataat ttatttagtg
     3421 gcgaatattc taataaaaaa acatagctgc agagcaagca attcggttgt tggcaagtca
     3481 agggcaaagc attcgcgtga gaatgggcgt atgctttgtg ccaaaagcaa acgttgctaa
     3541 ttctgcactg ttcagcgatg gcgcagtaga gacaggcact gccactgtcc agtcggcaaa
     3601 agcgaacact ttgccactgc ggggataaac tgattgaagc tctcgcgcaa aatcacttat
     3661 tcttttgcgt taataaaatg taggagatgg gtcattgatc ctacccacgt aatatggaca
     3721 caggcctaag cgaggttct
//
LOCUS       DAJTXY010000220         3674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTXY010000220 DAJTXY010000000
VERSION     DAJTXY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.224"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..209
                     /locus_tag="OO566_004097"
     CDS             <1..209
                     /locus_tag="OO566_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6607138.1"
                     /translation="YGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPVLQNE
                     ARQQKRGLWSDADPVPPWIWRHRK"
     gene            255..464
                     /gene="hha"
                     /locus_tag="OO566_004098"
     CDS             255..464
                     /gene="hha"
                     /locus_tag="OO566_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6607139.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            503..1093
                     /locus_tag="OO566_004099"
     CDS             503..1093
                     /locus_tag="OO566_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6607140.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1333..1593
                     /gene="repA"
                     /locus_tag="OO566_004100"
     CDS             1333..1593
                     /gene="repA"
                     /locus_tag="OO566_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6607141.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1817..1891
                     /gene="tap"
                     /locus_tag="OO566_004101"
     CDS             1817..1891
                     /gene="tap"
                     /locus_tag="OO566_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6607142.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1884..2741
                     /gene="repA"
                     /locus_tag="OO566_004102"
     CDS             1884..2741
                     /gene="repA"
                     /locus_tag="OO566_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6607143.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3441..3632
                     /locus_tag="OO566_004103"
     CDS             3441..3632
                     /locus_tag="OO566_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607144.1"
                     /translation="MTENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          989 a          898 c          929 g          858 t
ORIGIN      
        1 gttatggtcg tattctcggg caggtttatg caccagacgg aatgaatatt aatcagttta
       61 tggtccgggc cggggctgcc tgggtatatg agcagtacaa cacagatccc gtgctacctg
      121 tccttcagaa cgaagccaga caacaaaaac gtgggctctg gtcagatgct gatccggttc
      181 ccccctggat atggaggcac cgtaaataaa gcgatggcaa tcctatcatt catctgttct
      241 cagcgggaat aaaaatggca aaaacaaaac aggaatggct gtatcagtta cggcgatgtt
      301 catcagtcaa tacgctggaa aaaattattc ataaaaacag agacagttta tcaaacagtg
      361 aacgtgaatc atttaattca gcagcagacc accggctagc ggaattaatc accggaaagt
      421 tatatgacag gataccaaaa gagatatgga aatatgtaag gtaataattc agcaataatt
      481 aattgccagg agcagataaa taatgaagtt gattattttt attctcattg ttctgattat
      541 tgcaacgcta cttatccgga ttatcctcag aagtgtaaat cagcattctc ctttgctgat
      601 gcaattgcat gcggcaggta tacggactgg tgatgcagag cgaatactgt ccagcggtga
      661 atactggcaa cgtcagaaga cgctgctgac agaaagggaa gtcagtttta tgaaaggact
      721 gttcagaatt gtggatatga agcggtggta tctgtgtccg caggtacggg tcgcggatat
      781 cgtccagctg aacgggaata tccggccacg atcacgccag tggtggcagt tattcaggat
      841 ggtgtctcag tggcatgttg atgtggttat cgttgagctg cgttcgttca gtattgttgc
      901 agcagtagag ctggatgatg ccagccattt acgaccggaa cgcagacgcc gggatattct
      961 tctggaagag gttctgaggc aggctggtat tccgttgctc agaagccacg atgccagaaa
     1021 actgctgcag atgaccggag aatggctgaa tacaacaggg gctgatcagc agtcaccgga
     1081 acatcgtagc tgacgccttc gcgttgctca gttgtccaac cccggaaacg ggaaaaagca
     1141 agttttcctc gctcccggcg tttccataac tgaaaaccat actatttcac agtttaaatc
     1201 acattaaacg acagtaatcc ccgttgattt gtgcgccaac acagatcttc gccacaattc
     1261 tcaagtcgct gatttcaaaa aactgtagta tcctctgcga aacgatccct gtttgagtat
     1321 tgaggaggcg agatgtcgca gacagaaaat gcagtgactt cctcattgag tcaaaagcgg
     1381 tttgtgcgca gaggtaagcc tatgactgac tctgagaaac aaatggccgc tgttgcaaga
     1441 aaacgtctta cacacaaaga gataaaagtt tttgtcaaaa atcctctgaa agatctcatg
     1501 gttgagtact gcgagagaga ggggataaca caggctcagt tcgttgagaa aatcatcaaa
     1561 gatgaactgc agagactgga tatactaaag taaagacttt actttgtggc gtagcatgct
     1621 agattactga tcgtttaagg aattttatgg ctggccacgc cataaggtgg cagggaactg
     1681 gttctgatgt ggatgtacag gagccagaaa agcaaaaacc ccgataatct tctttaactt
     1741 tggcgagtca gaaagattac cggggcccac ttaaaccgta tagccaacaa tcaagctatg
     1801 cggggagtat agttatatgc ccggaaaagt tcaagacttc tttctgtgct cgctccttct
     1861 gcgcattgta agtgcaggat ggtgtgactg atcttcaaca aacttattac cgccaggtaa
     1921 agaacccgaa tccggtgttc acaccccgtg aaggtgcccg gacgcttccg ttctgcggga
     1981 aactgatgga aaaggcggtg ggcttcacct cccgttttga tttcgccatt catgtggcac
     2041 atgcccgttc gcttggtctg cgtcggcgca tgccaccggt actacgtcgc cgggccattg
     2101 atgcgctgct gcaggggctg tgttttcact atgacccact ggccaaccgc gtccagtgtt
     2161 ccatcaccac gctggccatt gagtgcggac tggcgacgga gtctgctgcc ggaaaactct
     2221 ccatcacccg ggccacccgg gccctgacgt tccttgcaga gctgggactg attacctacc
     2281 agacggaata tgacccgctt atcgggtgct acattccgac cgatatcacg ttcacatctg
     2341 cactgtttgc tgccctcgat gtatcagagg aggcagtggc cgccgcgcgc cgcagtcgtg
     2401 tggaatggga aaacagacag cgcaaaaagc aggggctgga taccctgggt atggatgaac
     2461 tgatggcgaa agcctggcgt tttgttcgtg agcgtttccg cagttaccag acagagctta
     2521 agtcccgtgg aatgaagcgt gcccgtgcgc gtcgtgatgc ggacaggcag cgtcaggata
     2581 ttgtcaccct ggtgaaacgg caactgacgc gtgaaatctc ggaagggcgc ttcactgcca
     2641 gtcgtgaggc ggtaaaacgt gaagtggagc gtcgtgtgaa ggagcgcatg attctgtcac
     2701 gtaaccgtaa ttacagccgg ctggccacag cttccccctg aaagtgacct cctcagaata
     2761 atccggcacg cgccggaggc attcgcacgc ctgaagcccg ccagtgcaca aaaaatcagc
     2821 gctacataca aaaaacaacc tcatcatcca ccttctggtg catccggtcc cccctgtttt
     2881 tgatacaaaa cacgcctcac agacggggaa ttttgcttat ccacattaaa ctgcaaggga
     2941 cttcaccgta aggttacaac cgttcacgtc ataaagcgcc agccgtcaac cctacagggt
     3001 gcaatgtatc ttttaaacac ctgtgtatat ctcctttaaa ctacttaatt acattcattt
     3061 aaaaagaaaa cctgttcact gcctgtcctg tggacagaca gatatgcacc tcccaccgca
     3121 agcggcgggc cccaaccgaa gccggtttag ttacaacatg caaacataaa cccctaaaaa
     3181 acccaagtcc aacgcagaac tgaaaccaca acgcccctcc tcataactga aaagcgccgc
     3241 cgccccgccc gaagggcggg aacagagtcg cttttaatta tgaatgttgt aactacatca
     3301 tcatcgctgt cagtcttctg gctggacgta tcgagtacac gctcgtaagc ggccctcacg
     3361 gcccgctaac gcggagatac gccccgactt cgggtaaaac cctcgtcggg accactccga
     3421 ccgcgcacag aagctctatc atgactgaaa acgggtatgg cttagcaggg ctggggatgg
     3481 gtaaggtgaa atctgtcaat cagtaccggc tgacgccggg cttcggcggt tttactccgg
     3541 tatcacatgt aaccacagcg tgccgccttc catgccgctg gcgcggcatc agaataatac
     3601 aagcagcatt taatgcattt gcaaaagtgt gagtaatatt aagtggtcgt ttcagggata
     3661 tcgttaagac ttga
//
LOCUS       DAJTXY010000221         3657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTXY010000221 DAJTXY010000000
VERSION     DAJTXY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.225"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(2..298)
                     /locus_tag="OO566_004104"
     CDS             complement(2..298)
                     /locus_tag="OO566_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6607145.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            1050..1598
                     /locus_tag="OO566_004105"
     CDS             1050..1598
                     /locus_tag="OO566_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6607146.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRNMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVEALQTKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISRATYYRLAK"
BASE COUNT         1133 a          691 c          816 g         1017 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tggtttttct actgaaatga ttatgacttc aataaccctt gaatgaagtc
      541 tattcggttt tctaggagac ctgttaaaaa caggacaata tcaggtgaat gactatgcaa
      601 tttaatagta gatttccaag tatctaagca gttttacaca aattctatgc agtatcggta
      661 tagtctcttt ttagcataac ttattgaaat atatagagaa tctgtgaagt ctcttttcag
      721 ggaactatgc agggtgttag tcgttcctct ctcgactcct agaaaagtgc tatatacagg
      781 actatataac gatatataga tgtttggtca catggtgtaa ctatcaagaa acttaacgat
      841 ttcctacgtg aaagttagaa agtgatggtt atagttaccc tcctaaaatc ttcatagctc
      901 cttcataacc agtcttaaaa ggacttcctt ttgagacact ttcaacgtaa cggtagaatg
      961 caccctcaag tagtctcaaa aggttgtttt tggattatga gacagctaaa cttatgagac
     1021 taacttttga gacttctaag gggaacatta tggcggtgat tggttattgc agggtatcaa
     1081 cacaagacca gactgtagac aaccagaaac tacagataga gaaggttcac aaggtcgata
     1141 aatggtttat tgatgaggct gtctcaggag ctacaaaggc caccagcaga gatggtttta
     1201 gaaacatgat gaactatgta aggtgtgacg atacgttagt tgtggtagct attgaccgtt
     1261 tagggcgtaa taccgttgat gtgctctcta acgtggaggc actacagact aaaggtgtga
     1321 gggttgtttc tctacgtgaa ggttttgact tattaacacc aatcggtaga gcaatgctta
     1381 ctatgatggc tggtttagct gaccttgaaa aagaccttat cagtgaacgt agaaaggctg
     1441 gtattgaacg tgctaaagct gaaggggtac atatggggag gcctgtaaaa gcttcttcag
     1501 aggctgtaca gacgcttatt tcacaaggta agacgaggtt gcagattcag gaagaattag
     1561 gaatctctag agcaacttat tacagacttg ctaaataagt tactgaatag ttacatggat
     1621 gtagctgata tttggctttt ttgcaaaggg gatcacatag ctggtgtaac aggtacttag
     1681 cagctacaga catctgtcag atatttttga agattctcag agagggaatt tgaagagggg
     1741 ctactatgat tttataaaaa ttttcagagt ctgaaaagtt actgtacaga ttttatacaa
     1801 actaaaaagg actcccgtag gtgattcagt aatgaattac ttcacgaaga gtccctttac
     1861 aagtcgattt ttaatcgtgg ctacgacaat ccacccatac cagacctaac cgtaccccac
     1921 cttaccaagc cataccctac cccgccagac tataaggaag cgtagagaac cgatgaaggt
     1981 gtctgcacag ttgcatctta aacaaccatc acagtgatgt caaatagttc ttttaagtag
     2041 gaggggttcc cctatggtca catatcccct ctctcccgct tagtgtccct tgatagtatc
     2101 tagagagtat gcctgtaatg ttagagagta tgcctgtaat gtttctgtat caggtcagct
     2161 ataagaggtc tggatattac cttgtaagat tcttggtagt gattatgcag agggcttgat
     2221 aagagtgttt aacgggagga agagaaccat gaaaccgaag gtaggttcta tcttaacagt
     2281 gactgtaaga gatacctgaa aaaatacttt aacagaacta ccaagaagag agggaacgga
     2341 agagagggaa cgataagacc agaggtagtg acctgttaaa caggctgttt caaagtctct
     2401 taaaagtctg ttaagagggc taggtgaaca atcctgccat tttgtcaagt gttttgtctt
     2461 tagcaaaaag tgctaaactt aacaaaaagc actaaatgct aaagaggcga ttaagaaata
     2521 tactaagagg ggcgcttcgc ttggggtcac tatgttcttt ctctcccgtt aagaggattt
     2581 acagggacat ttcaaagccc tgataaggta agcccgatga taacagaaac agagggcaaa
     2641 accacttatc aacactgtga acgaataacg taaagggctg gttaagaaag tcggtttagg
     2701 gattggtaac aataccaatt agaagggagc ttccctttct ggggtgtcgc tctatgggtg
     2761 aagttaacct ttgaagttct ctgtgaagaa actgaacagc tattatattg gctaattaca
     2821 accctttgaa agataagcat ttttagaagt tactgttaag gttgcagagt tgtctgtgaa
     2881 gagacttgtc agttctctat attgggtggt tacaactcat tgaaaaataa gagcttttag
     2941 aggttactgt cagagaggtg aatacttgat agatacagtc ttccggttct gtcaggctat
     3001 tttttaacag atagcacaaa atgctaaaga tgcccctatg ccatctcgta caggggggtt
     3061 ttgaagggag tttaacgggg gtctgtgtgg tcattatcct taaggtcaag cgtccatgtg
     3121 cgaccgaaca gtttaaagac aacaagcact ctgacgagga cgtaacgaag tagcagatct
     3181 gcaataagtc caatacctcc accagcccat acatcaggtg accccagctc aggagggaga
     3241 tttaccatta gttttccttg taaggcgctc tcaatcgcat tctgacacaa aaaagtacct
     3301 gaccttatga gttgacctga agaggtcatt taactcgtta cagagcgatt gaagaggtct
     3361 gtgtgcaatg caataaccat gtgaccagat acatagccac gcctcatatt ttactctccc
     3421 cataaaccca ttacaccgca gatgttacga aattgttaaa aatacttggt attagtcttt
     3481 gaaaatgcta gcattgtata tataagttaa cagaataata acatgttatg aggggcagaa
     3541 tatgaatgag agacgcagaa tttagagcga tgcttcaggc atcaagagaa cgcaacaagc
     3601 acaactccta cgcttacaca aacaacccca ccagctacga agtcccaacc tttagca
//
LOCUS       DAJTXY010000222         3576 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTXY010000222 DAJTXY010000000
VERSION     DAJTXY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.226"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            18..479
                     /locus_tag="OO566_004106"
     CDS             18..479
                     /locus_tag="OO566_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6607147.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(520..990)
                     /locus_tag="OO566_004107"
     CDS             complement(520..990)
                     /locus_tag="OO566_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6607148.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(1037..1756)
                     /gene="yehT"
                     /locus_tag="OO566_004108"
     CDS             complement(1037..1756)
                     /gene="yehT"
                     /locus_tag="OO566_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6607149.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1753..3438)
                     /gene="btsS"
                     /locus_tag="OO566_004109"
     CDS             complement(1753..3438)
                     /gene="btsS"
                     /locus_tag="OO566_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6607150.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          949 a          924 c          817 g          886 t
ORIGIN      
        1 ataaaatgga gttttacatg aaggctttca ataagctgtt ttccctcgtt gttgcatctg
       61 ttctggtttt ctctcttgct ggctgcggtg acaaagaaga atcgaagaaa ttcagcgcca
      121 atctgaacgg cactgaaatt gccattacct atgtctacaa aggtgacaag gtgcttaagc
      181 aatcttctga aaccaaaatt caatttgcct ccattggtgc aaccaccaaa gaagatgctg
      241 ccaagacact tgagccgtta agcgccaaat acaaaaacat cgcgggtgtt gaagaaaaat
      301 caacctatac cgatacctac gcgcaggaaa acgtgactat cgatatggaa aaagtggatt
      361 ttaaagccct gcagggtatt tcaggaatca acgtttctgc tgaagatgcc aaaaaaggta
      421 tcactatggc gcaaatggaa ctggtgatga aagccgctgg ttttaaagaa gtgaaataat
      481 ctgtcggcgg ccatgtttcg catggccgcc atacccgtct tagcttttct tcacatgctg
      541 gcgcgctgcc agtccacgca gaaaataacg taaaaattga tcgccgcatt cgcggaagtt
      601 tttatgatcc ggtgcgcgca tcatcgctgt aatttccggc atcgaaacgc ggaactgctg
      661 ttcggtgagg atagccagaa tgtcatcggt tttcagcgaa aacgcgatgc gtaatttttt
      721 cagcacgatg ttgttattaa tgcgacgttc cggctccagt gccggagcag actcatcctt
      781 gccgcgtttt tcataaatca ggccattgag gaatgacgac aaaacaatgt ccggacaacg
      841 ctgaaaaccc tcttcgtctt ctttacgtag ccagacggcg atctgttccg cggtggcttc
      901 gacattatcc agcgccagaa tacgcaccag gtcattatta ttggctttca aaatgtagcg
      961 cacgctgcgc agaatatcgt tacttatcat gaggccttca ggtgttgatg aggcaaaaag
     1021 ccattttagc agtcttttac aggccaatcg cctcttttaa gcttttcaga taacggcggc
     1081 tgaccggcac ggttaagcca ttacgcaaaa tcaactcggc ctggccgtta tcttccagac
     1141 gaatctcctg taaatgcgcg aggttaacca gatactgacg atggcagcgc agtagtggtg
     1201 tacgactttc cagggtacgt aatgtcaatt cggtaaagcc ctctttccct tcgtggctgg
     1261 taacgtagac accgctcatc cgactgctga caaatgccac atctttcatt tgcagcaaat
     1321 aaatccgact atgccccgta caagggataa atttcagcgc ctgttgattt tccggtaaca
     1381 gcgaaacatc ctgcttgctg cgctcctgac gcaatcgcgc cagcgttttc tccagtcgcg
     1441 cttcatcaat tggcttcagc agataatcaa aggcatgttc ttcaaaggct ttaatagcgt
     1501 attcgtcaaa cgcagtgaga aaaacaatat acgggcgatg ttccgggtca agcatcccca
     1561 ccatttccag accactgatg cgcggcatct ggatatcgag aaacagcaca tccgggcgca
     1621 gtttatgcac cgcgccgatc ccttccacgg cgtttgaaca ctctccaacg atttcaatat
     1681 cgctctgctc ctgcaaaaat atacgcaggt tctcccgtgc taacggttca tcatcgacaa
     1741 ttaagacttt aatcatgcct cgtccctcca tggtagtcgt aacgttattc gggtgtaact
     1801 atcaggctca caggcgacgc ttattccata gtcatcgcca aaccgttcac gtaaacgctt
     1861 atccaccaga ttcatcccca gcccactggc attggttacc ggttgataca aaccggcatt
     1921 gtcttcgatc tccagcatca aatgttgccc ctcacgtcgg gcgctgattg ccactcgccc
     1981 tgtatccagc agttgtgatg tcccatgttt aatggcgttt tccactatcg gttgcagggt
     2041 aaacgcgggc aattgctgct gggataattc ttgcggaata gcaatgttga cctgcaaccg
     2101 cgactggaag cgcgcctttt caatttgcag ataagcgttc acatgttcaa tttcgtcggc
     2161 gagagtaaca aactccgaag gccgctttaa gtttttgcgg aaaaaagtgg aaagatactg
     2221 caccagctgg ctggcctgtt cgctgtcgcg gcggatcacc gctttaatgg tgttaagcgc
     2281 attaaacaaa aaatgaggat tcacctgggc gtgaagcagt ttgatttctg actgggtgag
     2341 catcgctttt tgccgctcat attgcccggc aagaatctgc gccgaaagca gttgcgcaat
     2401 cccctcgccc agcgtgcggt ttattgaact gaataaacgg tttttggctt catacaattt
     2461 gatggtgccc atcacgcgct gattttcgcc acgcaacgga attaccagag tcgaccccag
     2521 tttgcattgc ggatgcaaag agcaacgata aggtacttcg ttgccatcag cgtagaccac
     2581 ttcaccggtt tcaatcgctt ttaaggtgta agtcgaagaa atcggtttgc cgggtaaatg
     2641 gtggtcgtca ccaattccgg taaaggccag caatttctct cgatcggtaa tcgcgactgc
     2701 accaatatcc agctcctgat acagcacctg agccactttc atgctgttca cttcgttaaa
     2761 cccctgtcgc aaaatgcctt ccgtcgaggc tgccactttc agcgcagtgg cagaaaaagc
     2821 cgaagtgtat ttttcaaaca tcgcgcgttt atcgagcaat atacgcataa acagcgccgc
     2881 gccgacggta ttggtgacca tcattggcgc agcaatatta ctcaccagac gcaccgcatc
     2941 ttcataaggt cgggcgatcg caaggatgat cagcatttgc accatttcag cgacgaacgt
     3001 gacggcaccg gcggtaatgg ggttaaagac tttatcagtg cgcccgcgac ggatcaggat
     3061 gctgtgtacc aggccgccga gcaatccttc aacgatggtt gagatcatgc aacttagcgc
     3121 ggtcatgccc cccatcgaat atcgatgtaa gccgccggtc agaccaacca gcccaccgac
     3181 gaccggaccg ccgagtaagc cgcccattac cgcgcctatc gcacgggtat tggcaataga
     3241 atcgtcaatg tgcaacccaa accaggtgcc catgatgcag aagatggaaa agacgatgta
     3301 gcagagaaat ttatgcggca gacgaaccgt gacctgcatt aacggtatga ataatggcgt
     3361 tttactcatt aaccacgcaa tgactaaaaa aacgcacatc tgctgaagca gcagcaacac
     3421 cagattaaaa tcgtacatac ccgcaaacca cacttccctt taaaacgcgt aacatacatt
     3481 gcctgcgttt aactttcttt gaactcttgc agaaaaatga gaattcgtga gtacgatcac
     3541 tcaaaatcgc ctggcaaaaa taaaatcacc ctatag
//
LOCUS       DAJTXY010000223         3514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTXY010000223 DAJTXY010000000
VERSION     DAJTXY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.227"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            289..807
                     /locus_tag="OO566_004110"
     CDS             289..807
                     /locus_tag="OO566_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6607151.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKE"
     gene            814..1047
                     /locus_tag="OO566_004111"
     CDS             814..1047
                     /locus_tag="OO566_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6607152.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            complement(1349..1816)
                     /locus_tag="OO566_004112"
     CDS             complement(1349..1816)
                     /locus_tag="OO566_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607153.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(2886..3077)
                     /locus_tag="OO566_004113"
     CDS             complement(2886..3077)
                     /locus_tag="OO566_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6607154.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            3142..3447
                     /locus_tag="OO566_004114"
                     /pseudo
     CDS             3142..3447
                     /locus_tag="OO566_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
BASE COUNT          844 a          787 c         1042 g          841 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcagggcgct gacactccct gataatctgc cggaaacgaa acaggcgctt gcagagagcg
      121 tcacacgcgg gatagacgct ctttatcatg tcggggagat aaaaggccgt caggatgtga
      181 ttcaggcgct cacagaggca gggcttgaag tggtcagggt gacgcgaagc agtatcagca
      241 ttgcagaccc gaacggtggg aagaatatca ggctgaaagg agcattctat gagcaatctt
      301 ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa
      361 atgctgaacg acgagttcag gaagctcgaa gaatctgtaa gcaaggctgt gacatcaaac
      421 gagacgaaaa tcaaagacgc tatagccctg ttcacagctt cgacagagga atcactgaaa
      481 aaacaccggg aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt gttaaagctg
      541 gcagggaata cgggcatgat gttactgggg atggtcattt tcctgtttac cgtgagtggc
      601 gggacgctct ggtatcttgg cgggatgata caggcgaacc tggaagaaat caggaagcag
      661 aacgagagtc tggagaagct gaacgcgaag acatggggcg tggagtttgt gcaggacggg
      721 aacaggaaat ttctggtcct tccttacggg aagtcagcag aggtgatttc ctttcagggg
      781 aaggaatggg tacatctgaa ggagtgacac agagtgacag agctggaaac acatttgctg
      841 aacgccttag agcagctgca acaggactat atgcagcggc tgagcgaatg ggagagcgcc
      901 ttcgtggaat tgcagaaaat gttttcgctt acgcaacggg acaacgcgat gctgaacgag
      961 cgggtcatgc agttgagtca gcaggtgcag cacttgagcg agcagacaga acgcttgagc
     1021 cagttataca gcgagaactg gagataagag aggaacggct gatacaggag cgcgaacatg
     1081 cgttatccct ggaacgggag cgtcagccgg aaatacagga acgcacgctg gatggtcctt
     1141 cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaggagg ggttaatgtg
     1201 gcctgtacca tctggttgca taggtattca tgcggttaaa atttatcagg cgcgatcgcg
     1261 gcagtttttc gggtggtttg ttgccatttt tatctgtctg ctgccgtgat cgcaccaaac
     1321 gcgtttcagt ggtgcgggca ataagatatt acgttaaatt gtgagagaat ggtccccata
     1381 tactgacttt atcatacgga tagggttggt agtgggtcga ttgggtttcg gcattgtttc
     1441 cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat tctcctgagc
     1501 ctggaaagtc taagtgttta gaattgatgg caaaatatct tccgtccgat tcttgaagta
     1561 gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt tgtagttgtt
     1621 gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt attattctct
     1681 cttttaataa gctgtttacc acaggattta gttgttgtct aaagcacgca gataatgcca
     1741 ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt gtttgatgtg
     1801 atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg gctataaaac
     1861 aaaattttca tagagtgatt caagatcttc ttgagatctt ttttggcggt gcgtaatctg
     1921 ctgcctgcaa acaaaaaaac caccgttatc aacggtggtt tgtttgccgg aatctagagc
     1981 taccaactct ttcttccgaa ggtaactggc ttggtggagc acagatacca aatgctgtcc
     2041 ttctagtgta gccgcagtta ggccaccact tcaagaactc ttaatatctc aatccacctt
     2101 atccagttac caatggatgc tgccagtggc gttaagtcgt gtcttaccgg gttggactca
     2161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
     2281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2461 ctatggaaaa acgccagcgg cgcagccttt ctttgccctt tgcctttttg ctcacatgtt
     2521 ctttccggcc ttatcccctg attctgtgga taaccgtatt accgtctttg agtgagctga
     2581 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
     2641 gcgccggaag ctgcattttc tccttacgca tctgtgcggc atttcacatc ggctatggtg
     2701 cacttttcat accatccgca ctgatgccgc ataagtaagc ctgtgtacac tccgctatcg
     2761 ctacgtgact gggtcatggc tgcgccccga cacccgctaa aacccgctga cgcgccctga
     2821 cgggcttgtc tgctcctgac atccgctcac aggcaagctg tgaccgtctc cgggagctgc
     2881 ttgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctcg
     2941 tcggcgtggt cgtggagcga ttcacagata tcagcctgtt catccgcgtc cagttcgttg
     3001 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     3061 ttttcctgtt tagtcactga tgcctccgtg taagggggat ttctgtttta tggggtaatg
     3121 ataccgatga atcgcgagag gatgctcacc atacgggtta ctgatgatga acatgcccgg
     3181 ttactggaac gctgtgaggg taaacagctg gcggtatgga tgcggcgggt ctgcctgggt
     3241 gagccggttg cacgctccgg gaagctgccg acactggcac cgccgttact gcgtcagctg
     3301 gccgccattg ggaataacct gaatcagaca gcgaggaagg tgaacagcgg gcagtggtct
     3361 tccggtaacc gggttcaggt ggtggccgca ctgatggcca tcggggacga gctgcgccgg
     3421 ctgcgtctgg ccgtcaggga acaggggcgc gggatgatag ttaagtttca tgcccgggga
     3481 aaaggtggtg gcagtggtcc ggttgattac ctgc
//
LOCUS       DAJTXY010000224         3490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTXY010000224 DAJTXY010000000
VERSION     DAJTXY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.228"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(109..966)
                     /gene="sitD"
                     /locus_tag="OO566_004115"
     CDS             complement(109..966)
                     /gene="sitD"
                     /locus_tag="OO566_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6607155.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(963..1820)
                     /gene="sitC"
                     /locus_tag="OO566_004116"
     CDS             complement(963..1820)
                     /gene="sitC"
                     /locus_tag="OO566_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6607156.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1817..2644)
                     /gene="sitB"
                     /locus_tag="OO566_004117"
     CDS             complement(1817..2644)
                     /gene="sitB"
                     /locus_tag="OO566_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6607157.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2644..>3490)
                     /gene="sitA"
                     /locus_tag="OO566_004118"
     CDS             complement(2644..>3490)
                     /gene="sitA"
                     /locus_tag="OO566_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6607158.1"
                     /translation="ASATEKFKVITTFTIIADMAKNVAGDAAEVSSITKPGAEIHEYQ
                     PTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSSGVTPVGITEGPYEGK
                     PNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKAKITQTLAPLRKQITE
                     LPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPAV
                     FSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKA
                     GKREK"
BASE COUNT          922 a          913 c          840 g          815 t
ORIGIN      
        1 gattttacgt attttttgca tgatagaatt cgtacctcct cccaacgacc ttccatggct
       61 ttccggactt ctgtaggctg gcggacgatg ctgtcctcgg atgttgtgct aattgggtga
      121 taacagatcc tgtgtatggg cgtttccctt tgtgcgtgcg ttgatgctgg taacggtaaa
      181 cgtcatgatg aacacgaccg cgaataagac gacaatcgtc ggagctggtg cgctgtcgat
      241 aaaaaaggac agccagacac cagtcattga cactattacc gatatgccag tcgccagtag
      301 gagcgcaata tgaaagcgtt gtgtcaggag tactgcaatg gcgccaggcg caatgagcag
      361 agaaatagag agaatgatac ctactgcttt cagcgtcgcc acaatggtca gggaaaccat
      421 gcacagcagc ccataatgca gccatctcgt atgcaaaccg cttacctgcg cctgttgata
      481 atcaaagctg aacagcaaaa aatcccgcca ttttacgcta ataacaagtg taaccagccc
      541 ggcaataatc accgtctgga ttatatcgcc gatggttatc ccgagcatgt cgccgaaaag
      601 aatatggtca agatgtacgt ctggtttaat tgcaatatac aagatcagac ctgcggcaaa
      661 catgccagaa aaaacaatcc ccataactgt atcttgcttg attcggctgt tgtctttcag
      721 gtatcccgtc gccaccgcgc agaatactcc ggcgacgaat gcccctgtag ccagaggtaa
      781 ccccagaatc caggtaagca cgatgccagg aaaaaccgca tgactcatcg cgtcacccat
      841 cagtgcccaa cctttcagga ccagaaagac cgacagcagc gcacagggta tggaaactac
      901 cagtgaaatc agcagtgcgt gactcataaa ggtgaactgc agtggctcca gtaataatgc
      961 catcatgagc gatcctccag cgctttatgc gcacgccgac gatttgccaa aagtccatgg
     1021 gtgggcgcaa atacaaatgc taaaagaaac agcagtgtct gcgccaccac gatgattcca
     1081 cctgtcgcgc cgtccagaaa gtaactgacc cacgcgccga ggaaactggt tacactgcca
     1141 atagttacag caattatgag caggcgggga aaacgatcgg ttaacaacca tgctgttgca
     1201 ccgggagtca ccaccagaca gatcaccaga aaggcaccaa ccgtttgcag cgccgcaacg
     1261 gtcgaaaccg acagcagggt gaagaagagg atttttaatc tgcccggatg taatccaata
     1321 gcacgggcgt gattctcgtc gaaaaaggtc accataagat ctttccattt gaaaaaaagc
     1381 acgatgatcg agagaatgcc aatgatagtg agttgcagga tatccgccgg atcgattgcc
     1441 agaatattgc ccaggacgat ggtctgaatg ttcacggatg tcgggttcaa tgacaccata
     1501 aacagcccca ggccaaaaaa ggacgagaag atcaggccga tgatggcatc ttcttttaag
     1561 cgggtgcgtt ggttaagaaa cagcatgctg cccgctgcca gtccgccgga aaagaaggct
     1621 cccagagaaa acggaagccc cagcatatat gcccccgcaa cacccgggac aatagagtgc
     1681 gacagcgcat cgccaatcag cgaccagcct tttagcatca gatagcatga caggaaagcg
     1741 cagagaccgc cgaccattgc cgagacccac atcgcattga gcatatattc atagctgaag
     1801 ggttccagta gcacgttcat cttgcttccc tctgcaccgc tgacggtcga tgtgccacaa
     1861 aagggcgttc atcgtcagta ataatgcttt cttccgagcc gttaagtgtg acatggcgca
     1921 gtacgccgct aaaagccagc tccaggttct cggctgtaaa agtggtgtct gtcggcccgc
     1981 ttgccaggac ggtgcctttg accatgaccg tataatcgca aaatgtcgtg actgagccga
     2041 gattgtgcgt tgagacaagc atcgttttgc cttccgcgcg taactccctc aacaggctga
     2101 tgattttcgc ttcggttttc acatcaacgc cggtaaaagg ttcatccagc aggatcacat
     2161 caccctgctg tgcaatcgct ctcgccagaa agacgcgttt cttttgtcca ccggaaagct
     2221 cgccgatttg acgatggcga aaatccacca tatcgacacg ttccagcgca tcggtgacaa
     2281 tctgacgatc cctctttttg gctatacgca acatacccat atgcccatag cggcccatca
     2341 tcaccacatc ttccaccagt acaggaaatg accagtcaac ctcttctgac tgagggacgt
     2401 aggcaaccag gtttttctgt agcgcctgtc gcgtgggaat acccagaaca gatatctttc
     2461 cgctggtcag acgcacaaat cccataatcg ctttaaacaa cgtcgatttt ccggaaccgt
     2521 ttaccccaac cagagcggca atagatccac ttggtactgt gaaggatgca tcgcgcagtg
     2581 ctgtgtgccc gttacgccag gtgacagtaa catcattaac gacaatgcct gcagattgca
     2641 tcattatttc tcccttttcc cggctttaat tccctgtacc agcgtactgg tagtaacctt
     2701 aagaaggtcg atatacgtag gtaccgggcc gttttctgtg ctcagggaat cgacatagag
     2761 cacaccgccg tagtgtgcgc cggtttcacg cgcaacctga cgcgctggtt tatcggaaat
     2821 cgtactctcg ctaaagactg ccgggatatg atttttctta actatatcaa caaccttacg
     2881 tacctgctgc ggtgttcctt gttgatcggc attaatcggc cacagataaa gctcttttag
     2941 ccccaaatcg cgtgcgagat aagaaaaagc cccttcactg gtgaccatcc atcgctgatt
     3001 ctcagggagt tccgtaatct gcttacgcag gggggcaagg gtttgggtaa tcttggcttt
     3061 ataagtatcg gcattacgtt ggtaggtttg tgcatttgcc ggatcgtatt ttatcagcgc
     3121 atcacgaata ttatcgacgt aaatcagagc attatctggc gacatccatg catggggatt
     3181 aggtttgccc tcatagggtc cttcggtgat ccctactggc gtcacacccg aagagacaat
     3241 tacttctgga accccattga gatgctggta aaagcgttgg aaccacaatt ccagattcat
     3301 accattggcg agaatcagtt gtgccccctg cgcacgttta atatcgccag gggtaggctg
     3361 atactcatga atttctgcac caggcttggt tatggatgag acttctgcag catctccagc
     3421 cacgtttttg gccatatccg cgatgatggt aaatgttgta atgaccttga atttttcagt
     3481 tgcacttgcc
//
LOCUS       DAJTXY010000225         3436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTXY010000225 DAJTXY010000000
VERSION     DAJTXY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.229"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..1433)
                     /gene="cusS"
                     /locus_tag="OO566_004119"
     CDS             complement(<1..1433)
                     /gene="cusS"
                     /locus_tag="OO566_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6607159.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIT"
     gene            complement(1423..2106)
                     /gene="cusR"
                     /locus_tag="OO566_004120"
     CDS             complement(1423..2106)
                     /gene="cusR"
                     /locus_tag="OO566_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6607160.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2263..>3436
                     /gene="cusC"
                     /locus_tag="OO566_004121"
     CDS             2263..>3436
                     /gene="cusC"
                     /locus_tag="OO566_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6607161.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKMEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQI"
BASE COUNT          843 a          896 c          901 g          796 t
ORIGIN      
        1 aatgtgataa caaaccttgt cccccgcgca tctgacgtta ccgcaaccgt gcctttatgc
       61 gcgacaacaa tcgatttcac tatcgccagc ccaataccgc taccttcacc ttttcgctgg
      121 cgggaggggt caacgcgata gaaacggtca aacaatcgcg gtaagtgctc gggcgcaatg
      181 ggcgtaccgg ggttttcgac ggaaacttgc acctggtgat cgaccgtctg acagcgcact
      241 acaattgtct ctctggttgg cgtataacgc agggcattag aaagcaggtt gcttagcgcc
      301 cgacgcagca tcaacggatc gcccgcaacc tgacgtttgt cgccaacaaa ccgcaactcc
      361 acgccgcgat cttccgctag cgcctcgaaa aaatcgaaca ctttgccgac ttcatccgcc
      421 aggttgagca ttttcttttc ggggattagc tggttgttat cggcctgagc gagaaacagc
      481 atatcgctga ccattttcgc cattcgcgtc agctcttcga gattagagta gagcacatct
      541 tccagctcct tctggctgcg cgactggctg agggcgattt ccgtttgcgt aatgagatta
      601 gtaattggtg tgcgaatttc gtgggcgata tccgctgaga aattggactg gcgagtaaag
      661 acatcctcaa tacgctcgat catatggttg aacgacagta ccagctgttc cagctcaatc
      721 ggcacggtct gcgggtcaag gcgaacgtcg agatctttcg aggtgatatt ctggatttga
      781 cggctgacgc tgcggatcgg cgcgtgacct ttatgtaccg ccaacagcac gataaagacg
      841 atcaggatgc tgattaccga tgcggtcata ataagtttat tcatcaaatc atttatgtaa
      901 tgaagatgaa aatcgatcga cagcgcgatg tagagcgtat aaatcggttt gccgtccacc
      961 aacgggccaa ccggcaagtt aatcatccgc cagttgctgt gttccatatg cccgtgaccg
     1021 tggcctggca tcataatcgt cgggccggaa aggagataca cctcgccacc ccgcgcgtct
     1081 ttatcgggta tggcatcacg tgcaaactcg cggatatcag gcgcaccggg ggagtgatac
     1141 accgttttgc cgtggctatc tgccagggaa atcaacacgt tggaataacc actgacgata
     1201 tcttccagcg tcattaagcg tcgggcttgc gtttcgtcag ggtgatttag tacccgttca
     1261 agggtggcgc taatctcttt taaatcatta atgtcctgct cggcaaaatg cacttttact
     1321 gagtggatca tgatccatgc aaaggcgaaa aacgccgcga tggtggccag gctgataaaa
     1381 aaggtcaggc gggttgccag cgaaaacggg cgctgaaatg gcttactgac catccggcac
     1441 ctcaagcatg taacccacgc cgcgcacggt ttgaatcagc ttcggctcaa agtcgttgtc
     1501 gattttgccg cgtagccgct tcaccgccac atcaatggcg ttagtgtcgc tgtcgaaatt
     1561 catgtcccat acctgcgagg cgataagcga gcggggcagc acttcgccct gatggcggag
     1621 aaaaaactcc agcagagtaa attctttact ggtcagagtg atgcgcgtgc cgctgcgggt
     1681 gactttgcgg ctgacgagat cgaccatcag atcggcaacc tgaaactgac tttcgataat
     1741 caccgccgcc ccgcgccgca ggagagtgcg cacccgcgcc agcagttcag caaaagcgaa
     1801 cggtttcacc aggtagtcat ctgcccccaa ctccagcccc ttgacgcgat gttcaatggt
     1861 gccaagcgcg gtaagcaaca gaatcggcat ccctttattg gcggagcgca acatgcgcac
     1921 gatatcccag ccgttcacgt ctggcagcat aatatcgagg attatcaggt cataatcacc
     1981 ggtcatcgcc agatggtagc cattcagccc gttgtcggcc aaatcgacca caaaaccggc
     2041 ttcggttaac cctttggtca agtattctcc ggttttcttt tcatcttcga caatcaacag
     2101 tttcatattt cctccgcatg ttgcccgggc aattctagag tagcgggatc agatggcaat
     2161 cgcttattgg caaaatgaca attttgtcat tttcctgtca ccggaaaatc agagcctggc
     2221 gagtaaagtt ggcgacataa aatcaccaga aattatgagc ctatgtctcc ttgtaaactt
     2281 ctgccatttt gtgtggccct tgcgctaact ggttgttcac tggcaccgga ttatcagcgt
     2341 ccggtaatgc ccgtaccgca gcagttctca ctcagccaga acggcctggt taacgcagcg
     2401 gataactacc agaacgcggg ctggcgcacc ttttttgttg ataatcaggt gaagacgctg
     2461 attagcgagg cgctggtgaa taaccgggat ttgcgcatgg cgacgctgaa agtgcaggag
     2521 gcacgggcgc aatatcgtct gaccgatgcc gaccgctacc cacagctcaa tggcgagggc
     2581 agcggcagct ggagcggcaa tcttaaaggc gattcagcca cgacgcggga gttctcgact
     2641 ggccttaacg ccagctttga tctcgatttt ttcggtcgct taaagaacat gagcgaagcc
     2701 gagcgccaaa attatttagc cactgaggaa gcccagcgcg cggtgcatat tttgctggtt
     2761 tctaatgtcg cgcaaagcta tttcaatcag cagctggcgt atgcgcaatt gcaaatagcc
     2821 gaagaaacgc tgcgtaatta tcagcagtca tatgcgtttg tcgaaaaaca actgttgacc
     2881 ggtagcagca atgttctggc gctggaacag gctcgcgggg tgatagaaag tacccgcagt
     2941 gacatcgcta aacgtcaggg ggaactggcg caggcgaata atgcattgca actgttattg
     3001 ggaagctacg gcaagctacc gcaagcgcag acagtaaaca gcggcagcct gcaaagcgtt
     3061 aaattaccgg cgggcttgtc gtcgcaaatc ttattgcagc gcccggatat tatggaagct
     3121 gaacacgcgt taatggcggc taatgctaat attggtgctg cgcgtgcggc atttttcccg
     3181 tctatcagcc tgaccagcgg aatatcaacc gccagcagcg atctgtcgtc attatttaac
     3241 gctagcagcg ggatgtggaa ttttattcct aaaatggaga tccccatttt taatgccgga
     3301 cgcaaccagg ccaatctgga tatcgccgaa attcgccagc agcagtcggt ggtgaattat
     3361 gaacagaaaa tccagaacgc ttttaaagag gtggcagatg cgctggcatt acgtcaaagc
     3421 ctggacgatc aaatca
//
LOCUS       DAJTXY010000226         3412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTXY010000226 DAJTXY010000000
VERSION     DAJTXY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.230"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            190..447
                     /locus_tag="OO566_004122"
                     /pseudo
     CDS             190..447
                     /locus_tag="OO566_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO566_004123"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO566_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6607162.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHKQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO566_004124"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO566_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6607163.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO566_004125"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO566_004125"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6607164.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OO566_004126"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OO566_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
BASE COUNT          871 a          813 c          819 g          909 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgcttgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttagcgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tg
//
LOCUS       DAJTXY010000227         3387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTXY010000227 DAJTXY010000000
VERSION     DAJTXY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.231"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..310)
                     /locus_tag="OO566_004127"
     CDS             complement(<1..310)
                     /locus_tag="OO566_004127"
                     /inference="COORDINATES: protein motif:HMM:NF013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6607165.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRD
                     Y"
     gene            complement(443..748)
                     /locus_tag="OO566_004128"
                     /pseudo
     CDS             complement(443..748)
                     /locus_tag="OO566_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            816..1431
                     /gene="tnpA"
                     /locus_tag="OO566_004129"
                     /pseudo
     CDS             816..1431
                     /gene="tnpA"
                     /locus_tag="OO566_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1759..2841
                     /gene="ompC"
                     /locus_tag="OO566_004130"
     CDS             1759..2841
                     /gene="ompC"
                     /locus_tag="OO566_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6607166.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKVNL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
BASE COUNT          962 a          753 c          701 g          971 t
ORIGIN      
        1 ggtagtccct gcggatattt gcgatacagg aatgcagtcg ctgtattttg cgtttctgct
       61 tctgccagtt gttgctgaat ttgaccttgc ggcttaactg tcgctgaagt ctcgccagcg
      121 tcttctggtt tttctgaaaa ctgtttacag gctcaaagac tgtgccatct gacagcgagg
      181 caagtttagc cacgccagca tccagcccga ccattgatgc tgaagggtga accggagttg
      241 atacttcact ttctgcctga atactgatgt accacttacc gcaggactgg ctgacagtga
      301 catttttcac aacacccgtg acctgacggc tgttccggta gcgcatccag ccaagttttg
      361 gcagaaaaat acggctgttt tcctgatcga gcttaacgcc ctgcggatag cggaatgcat
      421 cattctgtca ccgctttttg aatcagggga acgccgaccg cttctggaaa aagtttttgc
      481 aggcctgctc agggtctttc agtgactgtt gcaatggctg tgacggagca tctttaatcc
      541 attgcgtttc agtggcattt ttccactcaa ccaatcagga agccattttc gcgtaaggga
      601 tgtctttgtt accggcctta tgattctcat tctgacgtgc cagagtacga ctgaaaacga
      661 aacgacatgc gcctgcgaag cgcctcattt cacgcgcctg ttgaccaccg ggtcttaacc
      721 ggaatttgaa tacctgtagt cgtttcacag ggcaaattat actttggtct gtgaaaaaaa
      781 ctgatatccg acgcggaagg cactgtgttt tcctgatgca tgctcaccgg gtctttgtta
      841 ccaagtaccg acgccagatt tttgaccatg acgcgacaga aaaactacgt acttactttt
      901 caaatgtatg tgctgatttt gaagctgaac tggttgaaat ggatggcgaa ccagagcacg
      961 tccatctgtt aattaactat ccccccaaac tggcgatatc cagtctggta aacaaacagc
     1021 ctcaaaggcg tatcgggtag gttactgcga cgggatcggc cagatattgc tgccaggtat
     1081 taccgcaaag atgttttgtg gcgtcccggc tattttgcca gtagctgcgg aggtgcgcca
     1141 atatccgtca tccgccaata cattgaacaa cagcaaacac ctggtcaggt gaaaaaacga
     1201 gccttatatc cccgccctga aggacgggct tttacggtgc actggataag cctggagatt
     1261 atctacacct gcatggacat cattgtacta tgccccgtac atgccccatc acatcctcaa
     1321 catcaaagta ttagagcctt gcaaaccatt atgcgtctca gtttgtctca tctatcttac
     1381 gcaaaaaaaa tagaactaat atcaagcggc gctatattgc gtattaaata aaaccacctc
     1441 aattacatta ataaatctaa ataaatatca tacaaataat ttccatggtt atttttaggc
     1501 tatttaaatt aaaaaaataa attatgagtc aatcataaat aacaaccttt aatataattt
     1561 gactaccaaa tttatattcg ttcacaaaat agtcatgcat tgtgtgcaat agaaacagtt
     1621 cagataaaaa tagggatcta cttcacaatt taaatgagaa actaaaactt acatcttgaa
     1681 ataatctcat tgattggatg aatatttgta gtgcagtgac atcattttta aataatagtt
     1741 caaataaaag ggctcacgat gaaaaaatta acagtggcaa tttctgctgt agctgcatca
     1801 gtactgatgg cgatgtctgc tcaggcagca gagatttaca ataaagacag taacaagctg
     1861 gatctgtacg gaaaagttaa tgctaagcac tacttctctt ctaacgatgc agatgatggc
     1921 gacacaactt atgttcgtct gggcttcaaa ggcgaaactc aaatcaacga tcaactgact
     1981 ggtttcggtc agtgggaata tgaattcaaa ggcaaccgtg ctgaatctca aggttcttcc
     2041 aaagataaaa cccgtcttgc cttcgctggc ctgaaattcg gtgactacgg ctccatcgat
     2101 tatggccgta actacggtgt agcatacgat atcggtgcat ggaccgacgt tctgccagaa
     2161 ttcggtggcg atacctggac ccaaacagat gtgttcatga ctggtcgcac cacaggtgtt
     2221 gcaacctatc gtaacaacga cttctttggt ctggttgatg gtctgaactt tgctgctcag
     2281 tatcagggta aaaatgaccg cactgacgta actgaagcta acggtgatgg tttcggtttc
     2341 tccactactt atgagtatga aggattcggt gtaggtgcaa cctatgctaa atctgaccgc
     2401 actaatgatc aggttatcta cggtaacaac agcctgaatg catctggtca aaatgctgaa
     2461 gtatgggcag ctggtctgaa atatgatgcg aacaacatct atctggctac cacctattct
     2521 gaaacccaga acatgactgt ttttggtaat aaccatattg ccaacaaagc acaaaacttc
     2581 gaagtagttg cacaatatca gttcgacttc ggtctgcgtc cgtccgttgc ttacctgcaa
     2641 tctaaaggaa aagacttggg tgcgtggggt gatcaggacc tgattgaata tattgatgta
     2701 ggtgcaacct attacttcaa caaaaatatg tccacttttg ttgattacaa agtcaacctg
     2761 attgataaga gcgatttcac gaaagcatct ggcgttgcta ccgatgatat cgttgctgta
     2821 ggattggttt accagttcta atctgattac tcaaagatat gttgtgggag gctttgcctc
     2881 cccaacatat aagtggcgcc ctcaagccac tttccttaga agcactatct tgcttctgac
     2941 tatataaatt accctttatt ttctgggacg tttcaacgcc ccatttttaa taatactcag
     3001 tgaacaatta acgtatcaat tagatttaat aacaacgata tccgtaccta acctgatatc
     3061 taatgtcata agcatccctt taattatccc ctcaacctct tgtaaatttc ccactcttga
     3121 gctccacata gtccaaacgc cggatgcaaa aaaaatcaca cgtgcatctc tgaaattgtg
     3181 atggaggaag tgagtatcaa ttatatgtgc ttataaacgt tcccgattgg cacagaagtc
     3241 accggattgt tcaggctcca ataggttgag tatgtcgtat ttattttcca aaataaaaat
     3301 cgatgctgtt gctccttaat aaaatacttt tactctctgg acgcttttct tagctctctt
     3361 ggtgttatct taaaacgatt atggaac
//
LOCUS       DAJTXY010000228         3381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTXY010000228 DAJTXY010000000
VERSION     DAJTXY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.232"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..935)
                     /locus_tag="OO566_004131"
     CDS             complement(<1..935)
                     /locus_tag="OO566_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HCR6607167.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFIVYSFACFFIGKFIANILMT"
     gene            complement(963..1883)
                     /gene="rbsK"
                     /locus_tag="OO566_004132"
     CDS             complement(963..1883)
                     /gene="rbsK"
                     /locus_tag="OO566_004132"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6607168.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGMQSSYPSIEQFNEFLTLNE"
     gene            2189..2971
                     /locus_tag="OO566_004133"
     CDS             2189..2971
                     /locus_tag="OO566_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR6607169.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPALSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(2973..3199)
                     /locus_tag="OO566_004134"
                     /pseudo
     CDS             complement(2973..3199)
                     /locus_tag="OO566_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT         1002 a          772 c          672 g          935 t
ORIGIN      
        1 gtcataagga tgttggctat aaacttgcca ataaagaagc aggcaaagct gtagactatg
       61 aagtttgaag catcacgttc gttgatatcg cccaactcca gtgccagacg gatggtaaat
      121 gaccatactg cgacctgcat acccacataa aggaattgag ccacaatacc acgacgaaag
      181 cgcgaatttt tagccagata gcgcagcgta tccattgctg acgggcgttt atggtgactt
      241 gtctgtgcca ctttacaggt tgggaagcgg gttaaaagga acaacaccat gaccacaacc
      301 agaatcataa tcatatactt atacggttca agggtgttct ctaacatcag caccttaaag
      361 ttgtgaattt gctcggcgtt cattccggac atctgcttct caaggctttc cccctcggag
      421 aaaaccagat atttgcccaa taaaatacca gacgcagcac caatcggata aaaggtctgg
      481 ctgatattga gccgcaatgt ggcataggct tttggaccga tcattgaact gtatgtgttc
      541 gctgcagttt caaggaaact caggccaatc gcaatcgcaa aaatagctgc aaggaacata
      601 gtgtaggttg ccatatgcga ggcagggaaa aaaagtgtac aaccaccaat atacagcgtc
      661 aggccaatta aaattgccac cttataactg gtctttttaa tcacaaggga tgctggtatt
      721 gcaattaaaa aataacctcc ataaaatgcg ctctgcacca atgctgaagc aaagttactt
      781 agcgaaaata cacttttgaa ttgagtgatt aatatgtcat ttaatgcagc tgcgcatccc
      841 catagcggga ataaacacga taacaaaata aactggaaca agggagtctt attcagatac
      901 ccatcaggca tctgaatgat gtttttatcg ttcatagtgc tacctttaac tgtgcaggat
      961 gattattcgt ttaaggttaa aaattcatta aattgttcaa tactcggata agatgattgc
     1021 atacctttcc ctgtgacgct gaaagcggca aagagagcgg cttttttcaa agcggcttca
     1081 acatcaccgc tttgaacata ataatgggaa aagcaaccaa taaatgcgtc accagcgcca
     1141 ctagtatcaa cagcatttac tttgaatgca ggaacatgga cttcctgatc gcgggtcatc
     1201 cataatgcgc ctttttcgct catggtaaca ataatattgt tcagcccttt atcaactaac
     1261 gaacgtgcgg ccaaacgaat atgatcataa gtatcaaccg acataccagt taatatttcc
     1321 agttctgttt cattcgggat aaagaaatca cctttgcagg cataagacat atctaactca
     1381 cgcaatgccg gagccggatt taataacact tcaataccat ttttcttacc aaactcaatc
     1441 gcgtggtaaa ctgtttccag ttgaacttcc agttgtaaaa cgatcaattt gcattttttc
     1501 agatcttctg cagctcgatc gatatcttcc ggggaaagaa atttattcgc tcccttaatt
     1561 attaatatac tattgctcga gttggcatta acaaagatcg gtgcaacacc actgctggta
     1621 caggggacct tctcaacata agtggtatta attccccatg attcaagatt acgaatagta
     1681 ttatccgcaa aaatatcatc acctacttta gtcagcatca ggacttttga attcaattta
     1741 gccgccgcca ccgcttgatt agcacctttc ccaccacatc cgattttgaa ggcaggtgct
     1801 tccagagttt ctccttcttt aggcatctga ttagtgtaag taatgagatc caccatattg
     1861 gaaccaataa ctgcaatgtc catttcacta cctcttataa actttcgcat aacaatggta
     1921 tttaaataac attagcatgt tacttttgca tcatttgtga ctgagatcgc gattagcaca
     1981 tcaacccgat gtttatttaa tagacttcca gtctcatcac tcaggccaac actatctaat
     2041 cataagcaac ctaacaagat tagtgcccaa aattcagcag cctataccct tttcatttca
     2101 aaggggcggt cgtatagtat ggtaatgaaa acaatgttta ctaacgccaa aatgttattt
     2161 ttataacatt cttacggaga gagagttgat ggaaacgaag caaaaagagc gtatccgacg
     2221 tttgatggaa ctgcttaaga aaaccgacag aatccatttg aaagacgcag cgcgaatgct
     2281 ggaagtttct gtaatgacta ttcgtcgcga tctccatcag gaagatgaac ctctgccact
     2341 gaccctactg ggtggctata ttgtaatggt gaataaaccc gcgctatcca tgccagtaat
     2401 ccatgacgtt ccaaaaaatc atcgtgatga cttacctatt gcaattctgg ctgccggaat
     2461 ggttaatgaa aatgatctga tcttctttga taatggccag gagataccac tcgttataag
     2521 catgatcccg gatgcaatca ccttcaccgg catctgttac tcacatcgcg tctttgttgc
     2581 gttgaatgaa aagcctaatg taacagcaat actttgtggt ggtacgtatc gtgccagaag
     2641 tgatgctttt tacgatgcca gtaactcttc gccattagac tctctcaatc cgcgaaaaat
     2701 atttatttcc gccagcggtg tgcataatca ctttggcgtc agctggttta accctgaaga
     2761 tcttgccact aagcgtaaag cgatgaaccg tggactacgg aaaattttgc tcgcccgcca
     2821 cgcgttgttc gatgaagtgg cctctgccag cctcgcaccg atctctgcat ttgacgttct
     2881 gattagcgat cgtccgttac cggcagatta tgttacgcac tgccagaatg gttctgtaaa
     2941 gatcattaca cctgattcag aagacgaatg acttactgaa aaaacaccac aatcttgtta
     3001 aacatcgtcg ggttggactg attacgttgc actttcacca catattccag cttatctatt
     3061 tggcttatca cctactccag acgctggtca tccttgacca gtagccagat atggcttttg
     3121 tcgcagtcct gaatcggcag gcagagaatg tcttcaacgt taagagcgcg acgggcaaaa
     3181 gcccacaaag gtgagtcatt acacccggat ggtaaacagg cacttccttg ttgtagaagc
     3241 cgttatattg tccataatgt ctgggttttt aatgcatact gggtaacaac cggacgaatc
     3301 ttcgaatatc actggcgctg ccagccggtt tgaattcatc tcacaaccct gcatagagcg
     3361 aatctcttgc ctgcacgtca c
//
LOCUS       DAJTXY010000229         3258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTXY010000229 DAJTXY010000000
VERSION     DAJTXY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.233"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(195..2036)
                     /locus_tag="OO566_004135"
     CDS             complement(195..2036)
                     /locus_tag="OO566_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6607170.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2036..>3258)
                     /locus_tag="OO566_004136"
     CDS             complement(2036..>3258)
                     /locus_tag="OO566_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6607171.1"
                     /translation="VFQQENQAQRQKEFQQAYANAYASGDRGALRQLANQYPDQIESV
                     RKGMGFIDEDQRNSIGTLAAGARLAASSPEAMQSWLQNNANELARVGVDPNSVAQMYQ
                     QNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQARGQ
                     NLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGIETAV
                     NNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQNALV
                     NSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNEFKAE
                     WMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEPRNQV
                     GSYTSKSGIQFTVE"
BASE COUNT          741 a          824 c          716 g          977 t
ORIGIN      
        1 gccatagtga agagttgcga aggcatcgat acgatcacat tggctgtaat gtctgtcatt
       61 taatttgctc cagatacaat gaatcgccgc agcgtggcta cggtgaattt tgggcataaa
      121 aaaaaccagc cgaagctgga tagttgcgtt ggttatctgt cagtagttat gtgcttattt
      181 aattatttat ctatctattg ttctgctgta atttggcttg aaagtatagg gcgtatcgca
      241 tttgcagcat tatttaacgc tctttcataa gctgccgttc ctgctttggt gtttgccaaa
      301 cgaagaagca tattccttgc ggctttggac tcatacaaac gcatcattgc accaaaacca
      361 gcctcaagcc ccattgatac accaagagtc gcagttgcgc caatcgtcct tatcctgttg
      421 gcttgcgatt gccccgtctg agttactaca tttgcggtgt ctgaccttgc tgtttgctgt
      481 agaacttcat gaagagcatc aagctctttc atgtgctttc cagaaaaaat agtgttgtaa
      541 atttcaccgc ctgactgaga tttcagctta ttaacttcag tgatgaactt ggctggagag
      601 tcaccggcct tttccgctat tttgctgacg taagctgcac gcatagcatc tttcccttta
      661 tcatccagtg cgctccagat tcgtttcacg tcagatggtt ttctgcttaa tacaacggta
      721 tttataagtt caggactggc ttcactgctt gccttgttga gcttgttagc aatgttttta
      781 ttaagcacct tattataaac gtttgcataa tcggaatttg ctttaaggta ttttgctgcg
      841 tctgatgcac cgaggttttt agcaactgcg ttacgaaggt cttttgacat tgcattctct
      901 accatattgg tagctgcttt tgcctggttg gggaagacca tagcatctcc ctgaacatta
      961 gatctaaatg ctgttctgtg ctgacgcaag agatcaaacg taacatccaa atcagttgca
     1021 gggtttgcta attcttcacg taggttacgc aaggatgtaa gcaggctttg attggcagac
     1081 gtcccaagcc gttcctgtct tgcgatcgct gtattcagag cattcatggt atttgtggta
     1141 tcaactgcgg cattacccat tttattggtg acgtcattga taacagcgcc agcggcatcc
     1201 ttccgccccc ttaacgtggt ggtaagagat ttcaccacat catcagggtt gtactcacca
     1261 aaacggtcaa aataattact taccagctta ctacgcgttg catattgctc cgctcgcttt
     1321 gagcctgtcc cgagcaaagc cccctcggca tcctgagtta ggccgcgagt gaaagcattt
     1381 ttcggcggga taacatcaga tgtcattggt gtcacgccca tcgattctga tgtggcaatt
     1441 ttcttcgcca cttctggcgc aatatcacct tttatagccg ttattccacg ccctattccc
     1501 tttgctgctg cggaaagaac accctgagcg gcaaggttaa ctccggcatt tttagctgca
     1561 ttttgtgcga aatcgccttt ctgatttgcg gcctctgcca gtgatccaat agccatgctt
     1621 cctgccgttc caactcctgg aactaaatac ccaccaattg tttcaccggc ttgcgcgtag
     1681 gggtctgtcg gtctgtctac tggacgataa acatcatcca aaaccttggg tccaccaagc
     1741 ccctgactga ttgcattaat cagacttgcg ccgccctgca atacgtcaaa tggtatgttt
     1801 accagaccac gaccagcctg ttctgcaatt tgccctgcac tttgaccacc agtgagccaa
     1861 tcgccagctt gttgcatcaa tgatggttct tcccgtgttg gtgcattatt ggcctgatta
     1921 actgtttgtt gctgaacagc ctgaccagca aaatactcat caatggcggt gccaatatct
     1981 tcggtgctcg taccatcagg aaaggtaaat gtcttaccgt ttgcagttac tttcatcatt
     2041 ccaccgtaaa ttgaatgcct gattttgagg tatatgatcc aacctgattc cgtggttctc
     2101 ctgaaggtgt cgaatcttgt gctggcgctg cgtcagtatt cattgacata taccgcttaa
     2161 cggcactccc caatgattca ccttttttaa catccaaccc caatatctga ccgccattac
     2221 gcgattgtcc agggttgcca ttcgcgctca tccactcggc tttaaactca ttaaactgcg
     2281 cgtttcgtcg ctcaaggttt gccattgcat caagccatct tgcgaccgtc tcagggttat
     2341 ccatgtcagt tggcgcaccc tgtcgaacga tctcaacgtc tttatccgtt gctgggccgg
     2401 gaggtaggaa tttaagaacc tgactgttaa caagggcatt ttggcgaatg cgcaaatcac
     2461 gcaatgttgt atcgcttccg gtaagttttg cgaacatgtt ctgtgcgtta ccgaacaaac
     2521 ctgtcgttgg tttttctgct ctgaactgtt gagcaagcgc actcatagaa ttggctgagt
     2581 ttgatgatgc tgtagcattg tttacagccg tctcgatgcc tttttccatg tttactgaca
     2641 gcttaggtgc ttcgctaatc aactgctgag ccttttcctg cgcttgctgc atcttaaacc
     2701 cgaactcttg ctgatccaga gccaagcgtt gtgctgcgat attgtgccca gtcattgctg
     2761 actgatagga aaggttttgc cctctcgcct gaagtgcttc tccagcctga ttgctgcgga
     2821 ttgtctctgc cagcctgcct cggtcaattt cacgaccagc catcttgtcc tgaacattga
     2881 agtaatcaat cggaccaaga gcagccattc caaggtgatc aacaaactca ccaaatcctg
     2941 aaggattctg ctgatacatc tgagcaacgc tgttagggtc aacaccgacg cgcgccagct
     3001 cattggcgtt gttttgcagc catgattgca ttgcttctgg agacgaggcc gcaaggcgtg
     3061 cgccagccgc taaggtgccg atagaattac gctggtcttc atcaatgaat cccatgcctt
     3121 tacgaacgga ttcaatctgg tctggatatt gattagccaa ctgacgcaaa gcaccgcgat
     3181 caccagacgc ataagcatta gcgtacgcct gctgaaattc tttctgccgc tgagcctgat
     3241 tttcctgctg aaacaccc
//
LOCUS       DAJTXY010000230         3203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTXY010000230 DAJTXY010000000
VERSION     DAJTXY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.234"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..801)
                     /gene="ipaH9.8"
                     /locus_tag="OO566_004137"
     CDS             complement(<1..801)
                     /gene="ipaH9.8"
                     /locus_tag="OO566_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6607172.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQ"
     gene            complement(1063..1158)
                     /locus_tag="OO566_004138"
                     /pseudo
     CDS             complement(1063..1158)
                     /locus_tag="OO566_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1194..1499)
                     /gene="ccdB"
                     /locus_tag="OO566_004139"
     CDS             complement(1194..1499)
                     /gene="ccdB"
                     /locus_tag="OO566_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6607173.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1501..1719)
                     /gene="ccdA"
                     /locus_tag="OO566_004140"
     CDS             complement(1501..1719)
                     /gene="ccdA"
                     /locus_tag="OO566_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6607174.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2255..3203)
                     /locus_tag="OO566_004141"
                     /pseudo
     CDS             complement(2255..3203)
                     /locus_tag="OO566_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          791 a          774 c          782 g          856 t
ORIGIN      
        1 ttgtttgttt tccgggaacc atgctgtcac ggcatcagcc agggggcgat ggagtgtatt
       61 ttgttgtccg tcactcatgg agaagtaaat ccgtgggccg tggtagtccg gcgaagaggt
      121 taatctttgc agggcttgca gagcatggga tgataatggg ttatcactaa tatgtattga
      181 acattcattc ctcagattaa gaatgctttc cgggatatga cttatctgat ttctatcaaa
      241 aaaatactct ctgacaacgg gattatttcc ctcagaaaat gctggtagct cagtgaggaa
      301 attacgggtc gctctgagat ttttaagagc ctgtgggagc gatggtaagg agactatttc
      361 gttatagctg atattcatag tcaataacga atcgggtaaa gctggcaggt tttccagctc
      421 attgtgttgt acctgaagtg actccagcgc aggaggtagc acgggcaatt ctgataattt
      481 attgctggcg gaatataatt tttttagcgt aacaggcagt tcaggtaggt tagttaattg
      541 attatatgat acattgagca gcgttatctg agctggtaag ttgtcaggta gcgaggacag
      601 atttaaacgg tccagtcgaa gttcatcgga attattgata agacattctt taagtcggga
      661 gacagcctca tcacgctctt caccggggag cgcttgtttt tcccatttat cccatgctga
      721 aaagtaatca gcatatgtac cggaaatagt gttataaaaa gaattttggg gcaatgaaaa
      781 gttattattt atcggtaaca tgatgggcat ccagttaaag ccggtggaca aatggctttg
      841 ttaaaagaaa taagtaggag tttttatgaa gttcattcca tgaaatgaac ttcagcactt
      901 ttttattata ataaggataa acaaaattgg ctgtaaaaaa acgatactgt ttcagttaac
      961 cgtgaatggc ctgtttgcac cgtattgttc ttgtttttcg gttagttaaa catcgtactt
     1021 catatttgaa cggtaagcgg ctcgtcagaa ccgtattgat atttactgag aggtcagatc
     1081 aactttccag ggcaacagat cgcgtacccg gtttgccggc cagtcctgga tatgctcaat
     1141 gacgtaacgc agccattttg tcaacgccac gatttttgac cctagcctga catttatatt
     1201 ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc
     1261 acttcttccc cgataacgaa gatcggcaca ctggccatat cagtggtcat catgcgccag
     1321 ctttcatccc cgacatgcac caccgggtaa agctcacggg agactttatc tgacagcagg
     1381 cgggcactgg ccagggggat caccatccgt cgtccgggcg tgtcaataat gtcactctgt
     1441 acatccacaa acagacgata acgactctct cttttatagg cgtaaacctt aaactgcatt
     1501 tcaccagtcc ctattctcat cggcaaaaga gccgttcatt tcaataaaac gggcgatctc
     1561 agccatcccc tgctgatttt cagcctgcca gcgttccgca cgcagacggc gggcctcttt
     1621 ttgcatggcc gtatttacca ggcctgagat attcacgttt gcggatttaa gtaactgata
     1681 gctgtcactg tctatagtga cagtaatacg ctgcttcatg ataagccctc aaaaacatac
     1741 ttatgatatg tatatgagta tatattaatg gtacaaacca tcaatatcca taaaaagtac
     1801 accattaatt cgctataagt ttttaataca attttttctg ctgcgatttt tttatactat
     1861 aattagcata cttataacac cgtaagtaaa ttacatgaaa gcaggcacag cgtgatccga
     1921 tttttctgcg tataacgatt ttccggaagt gtaatacggt tgcgccccgg gccgggcacc
     1981 cttttctgtt gttgtgtacg ggcccacaga gaatggtacc ggaggcgggc tcagaagtac
     2041 tgagtcgggt gatcgcgtca ggccgcaaag tactgacacg ggtattggtg actggtgatg
     2101 aagtactgcc ccgggtatga tttcaggctg attcaggttc tgattctctg tcagtccgcg
     2161 tggttgctgg cctggctgat ttcctgttgt caggcgttca acaaccgact gcaggttata
     2221 gcccggttca cgaacacctg acggcaggta tcactcaccc ggtgaaggta aatccctcaa
     2281 gaggcagcaa ctctgccagt cgctcctccg gccattccgg cagacgcgtc aggacgtccg
     2341 tcagccaggc atgtggctcc agaccattac gttttgctgt ttccagaagg ctcattatct
     2401 gtgccgcccg gtttccggcc atcagtgaac cggcgaacag ccacgctttc cgtcccataa
     2461 caaccgggcg aatggcacgc tcgcagatat tgttgtccag gggcaccgct ccgtcaccca
     2521 ggaagcagct cagttcatcg cgccggttca gaatatagtt aatcgcctta tttaatggcc
     2581 cgtcgggcgg acagcacggc tcctgctctt caagccaggc aaacaggtca ttcactatcg
     2641 gccgggagta ccgttatcgc cactgccgta ttttttccac agggcgctca cggatgagtt
     2701 tctcaatgcg gtaaagaccc gcgatttttc tcagctccac gcctgcacgg ggctccctgc
     2761 tggccttata cagatttgcg aagccgcgcc ctgcgtgact ccagcaacag gctgatgtga
     2821 tgccggggtg attatccgcc agagatttgt acacgctgta tccgtcactc accagcgtac
     2881 cgccacacca gcactgcagc caggtctcag ggtatctgag ggcccgtccg gtctgcgagt
     2941 caaaacagac gaccggcgga ccgcttcttt caccgctgac gtaagcacac agccagccgg
     3001 agcaggattt accgcctttc cgggtatcaa ggagcctcag ggacgtttca tctgcatgaa
     3061 taacatcccg tgtcagaagc tcatggcgta gcagtttcgc cagcggggcc agtgcagctc
     3121 cggcaacacc gaccatatcc gccattgtac tgacggggag ctccacaccc attcgggaga
     3181 aaatgtgctg ctggcgatac agg
//
LOCUS       DAJTXY010000231         3176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTXY010000231 DAJTXY010000000
VERSION     DAJTXY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.235"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            846..1040
                     /locus_tag="OO566_004142"
     CDS             846..1040
                     /locus_tag="OO566_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6607175.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESEIGT"
     gene            complement(2065..3174)
                     /gene="fepE"
                     /locus_tag="OO566_004143"
     CDS             complement(2065..3174)
                     /gene="fepE"
                     /locus_tag="OO566_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6607176.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          941 a          781 c          657 g          797 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagata tttaacataa tatcttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc ccctaatata agatgatgtt tttttatgaa
      421 aatagtcagc ccccccccct aaaaagcggt gttggcgcag ctacaacacc gcgccgacac
      481 cgctttttta aatatcacaa agagagcaag aagaactaat ttgtcataac tctctattta
      541 taaagaaaaa tcagcaaaaa cttgtttttg cgtggggtgt gggcatttca ggcatgagaa
      601 cagactcgcg tttttccgga gtatcagcgg aattattgat taaggaacac tcgacgcaga
      661 gcgaaatttt atgcaacagc ggctgcactg cacagggatt atttatcagt tgatatgctg
      721 tgccatgata cgtcctaaag aaacaggaca ggatgtgcaa ttgtaatacc gtcgcccgca
      781 acgtcttaca attaccacct gctcagcccc ccccgacacc cggaactgac ggagaacaac
      841 ggaaggtgaa caaacaacaa caaactgcgc tgaatatggc gcgatgtatc aaaagccaga
      901 gcctgacact gcttgaaaaa ctggatgctc tggatgccga cgaacaggcg gccatgtgca
      961 aacgactgca cgaactcgca gaagaactcc agaacagcat ccagattcgc tttgaggccg
     1021 aaagtgaaat aggaacataa cgaagctgac ggaggcggtc acggctttcc cgtgggcgtt
     1081 ttcgtcagca gacaagcccg tcagggcgcg tcagggggtt ttagcaggtg tcggggcgca
     1141 gccatgaccc agtcacgtag cgatagcgga gtatatactg gcttaatcat gcggcggcag
     1201 agcaaatggt gagagaacag cgacatatga ggtgtgaaat gctgcacaga tacgtaagga
     1261 gaaaatgcag cttccggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1321 ttcggctgcg gcgagcggtg tctgctcacg caaagacgga tatacggtta tccacagaat
     1381 caggggataa caccagaaaa aacatgtgag caaaaaagag gccccgaaaa aaggccgcgc
     1441 cggtggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaac cgacgctcaa
     1501 gtcagaggtg gcgaaacccg acaggactta aagataccag gcgtttcccc ctggtggctc
     1561 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
     1621 gcgttcgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
     1681 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1741 ttgagtccaa cccggtaaga cacgacttaa cgccattggc agcggccact ggtaaacagg
     1801 atgtgcgaga gatgtattga gagttcttga agtggcagcc ttactgaggc tacactagaa
     1861 ggacagattt ggtgacatgt ctcgcacaag actgttacca cggttcagaa gttccccaac
     1921 ttactgaacc ttcgaaaaac cacctcccca ggtggttttt tcgttttcta gagtagaaga
     1981 ctacggctgt aagggaatga tctcaagaag atcctttgaa ctttttataa aagttgctta
     2041 taaaatgatg catcagcaca acttttactc aatcacactc atctttctgg cctccatggc
     2101 atgacgtaaa agtatatagc cacatgcaag catccctcca atgacagaag aaagaattac
     2161 aattatggca ttacttggcc catcctttct gaccggtaac gttgggctca attgatactg
     2221 aaatggcata aaatcaacat cccccacttt cgccacaacc aattgattca caacatattg
     2281 acgatttcgc aactccccac tcaattccga aacatccttg attgattttt tgatgttcaa
     2341 tttagttgct ataccatcag aaccgagagc cacaggaaaa tctgggtcat ccttcataat
     2401 ctgaccattg ctgtatacag gtttttttat tccagccgca ttagcaacct ccagtgaata
     2461 attgaggcgt cgaatatctg cattcagctg gtttctaatt tttatctcat ccagtgcaag
     2521 aatttctttt tcaacattag ttttaacttc tagtttattt cttatatctt ccatcaaatc
     2581 ccttacaaca agggaagaaa cataattaat atacccagac aacacatcgt gagcttcttt
     2641 acttgttgga gcagtaaaac tcagagtcca tgatacatac aatgatgact tgtcgttttt
     2701 cttactggca ttatcatcaa tcgacttcat atttttactc aactcaacaa ttgcacgatg
     2761 cagttcgaga gcgctaatgt tattcttttt aataatctcc ataacataag gagatgatct
     2821 catatactca ttcaacgaat taacggactg aaatttctta ataaacagag caaatgcgct
     2881 atcacgattg atatcaaaat ccactcccag aacatgaagc ttggaaagct ccttatttaa
     2941 ttcactccat tgaacagact cagcaggagt aaccacagca gaactagtcc atttttgtgg
     3001 caaaacaaaa gaaacaagaa agccaataca agcaaaaaat agaatcaccc caacaatttt
     3061 tatttttgct gcccataaaa cgctaatcaa gccaaaaagg tcaatttctt cattaccatt
     3121 cggcaacatg ttgtaattag aaaaatgttc gtcagagacc tttgagattt tcattg
//
LOCUS       DAJTXY010000232         3125 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTXY010000232 DAJTXY010000000
VERSION     DAJTXY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.236"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            773..1213
                     /locus_tag="OO566_004144"
     CDS             773..1213
                     /locus_tag="OO566_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607177.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(1292..2989)
                     /gene="treA"
                     /locus_tag="OO566_004145"
     CDS             complement(1292..2989)
                     /gene="treA"
                     /locus_tag="OO566_004145"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6607178.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
BASE COUNT          784 a          715 c          764 g          862 t
ORIGIN      
        1 atcagcattc tctcgtttca gtgtcgtgaa tatactgtta acggaggtga aaagaatgct
       61 gggggttata caaaacctat gttgttgtcg tccggattca ccgcttgctg atagtaagca
      121 gcgtaaccag aagttaccgc aacaagactt tgaggatttt aagaacgacg ttattaatgt
      181 cattaacaac aatgaagata tttattttct tagagagagt aatttactga atgcgtttat
      241 tcgaacgtgc aaaaaaagag atgataacag ttacggaaat atgatattta cgctttattt
      301 tgataacgat atctatggga acagaattat caaagcatcg ctgaacggcg gagagttgca
      361 gttattaagc acagggtgta gcaatgataa cattctccat tttattaatg catttctcat
      421 tcgggtgaaa tttccgctac ctggacgcat aaaaccggaa ctctcgccaa aagccgggca
      481 ctccgaagga attgacgcgt tgcgctgctc tgtaacggca tttaccgcaa gccatttatt
      541 tcgccagcga caatccgcag agaaccaaac gcatcaggaa acgactgaac acaataaata
      601 ctgtattgat caacatatta tactggcacc tccagcacgc agtgaggata cactgtatca
      661 tcagcgaatc ttatcactgc ggagctggtt gaacgacttt ctgagcgaaa taagtcggtt
      721 cccggtaaca acaacagaat ccttttttaa ttattgtttc gttgtttttg gtgtgattca
      781 gaaaataaat attttagaca gtagccagtc aggcgttaaa aatggggcgg atagtgaggc
      841 ggttttggct gcgcttattg aacatttaga attgatcaat ccttctggca gattaagcca
      901 aaatacccgt tcggcaatgc ttcagttacg cgaagaggaa tggtctgaat tctttttttg
      961 gttgctaaat tctctggagt gtctggatta tgtgattatt aatttaactc cagaaagcaa
     1021 aaaaacgttg atgagtgaac accgtaacaa tattcaggta gcaattgatg ctttatatag
     1081 ccaacgtcgg cgtaaatcgc cgggtgatga aagtgaaaca cttacccgta gaaatgacgc
     1141 tatcttcggc aaccacgtat ggcaaacatt tgcgcaatac ttcccgccag gacttgaaaa
     1201 accgtctgtg tagaaaaaac catagatatg gtgttatctg ccgacaacgc catccggaga
     1261 agccgaatga cgttgtcgga gtaagcgctg gttaaggtgt gggttgtgcc tctttggttg
     1321 agggttgcgt cgttgctgac ttaacggtcg gacgcgtcgc cggaacattg tcacacggtt
     1381 gctctttcgg gcagatcaaa tccagcattt tcagcgtcac gccattggtc cagccaaagc
     1441 catcctgtaa tggatattcg ccaccgccgc cccccgttcc ggtggtgctg acatcatatt
     1501 tttccaccag ctttttctcc cggtcatagg tgtgctgaac attggtcagg aagtgccagc
     1561 taatgtccat cgccacctct ttttgcccgt agttttgtaa tccttctgtc gcgacccact
     1621 gtaacggtgc ccagccattt ggcgcatccc attgttgccc acttttcacc gacgtggtgt
     1681 tcaggccgcc gggttgcagc agatgtgttt tcgtcgccgt cgccattttg ttggcgcgat
     1741 ctttcgctgc cgcattgacg tacagcggga acagggcggc cgcggttaac tgattgcgca
     1801 ctttatgact tttcaggtcg taatcggcat accagccttg ttgatcgttc cacaggtatt
     1861 tttcgatccc tttttgacgg gcattcgcca gcgtttcgta ctggtttgcc atcgcgttat
     1921 ctcctgcagc tttgctggcg cgggcgagga ttttttccat tttaaacatc aggctgttca
     1981 gatcgaccgg tacgatgctg gtggtgcgta aggtatttaa ctgctgcggg ttgtccatcc
     2041 agcgcgagct gaaatcccag ccagacgcag cggcagagcg caggtcgcgg taaatttcag
     2101 tggcaggtcg attcggattg cttttggcgg tggcaatatc ttccacccat gactctggtc
     2161 gtggcgtatc gcgatcgtcc cagtagcggt tgagaagggt accatcctga agttttacaa
     2221 cgcgtttttc ctgttgtccg gcttgcaggt tttcaacacc gtccatccag taagcatatt
     2281 ctttttgcat ttgcggcagg tattgcttca acgcggcatc gccttcatgc tgcgccagta
     2341 actctaccat cagggcaaag aagggcggtt gcgagcggct taaatagtaa ctgcggttgc
     2401 cgttgggaat atgaccgtaa gtgtctattt catgagcaaa attggccacc atatccgcga
     2461 ctttatccca gtgaccgctt tcggcaagtc ctaacatggt gaagtaactg tcccagtaat
     2521 atacctcgcg aaaacgtccg cccggcacga cataaggttc cggcagcggt aacagagaat
     2581 cccatttttc ggtgttttcg gtagaacgcg ttaataccgg ccaaagtccg tcaatatgtt
     2641 cgcgcagtga ctgcccctct ggcggaacat atttctcgcc ttctttcggc agggtgaaat
     2701 tgacgttaac gaaatggcgc agatcaaatc cgctctggtt ttgctgcatc cgataatcag
     2761 caaggatcat cagcggatcg ctgttcggca cggcatcggc aaaggttttt tggtccggaa
     2821 aaagtttggc gttttgcaca tcattaaaca gcggccctaa taaaatatca ggcggctgtg
     2881 gtgttaccgg tgtttcttct gcctgcaccg atagcgcagc gaaacacaaa aagatacagg
     2941 ctggaattaa cgccattttt tgcgggcgag aaggtgcggg ggatttcatc aatcattctc
     3001 ctttggcgaa accgaataaa acggtattca agtatcagaa aaccttagtt caggatcgca
     3061 ctagctgcat gatcgaaatc tcattctgcg aacggtcagg cattttagaa aagggaatta
     3121 aaggg
//
LOCUS       DAJTXY010000233         3049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTXY010000233 DAJTXY010000000
VERSION     DAJTXY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.237"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO566_004146"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO566_004146"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6607179.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO566_004147"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO566_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6607180.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3049)
                     /gene="bcsE"
                     /locus_tag="OO566_004148"
                     /pseudo
     CDS             complement(1963..3049)
                     /gene="bcsE"
                     /locus_tag="OO566_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
BASE COUNT          763 a          786 c          805 g          695 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aagcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgccctta
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctca
//
LOCUS       DAJTXY010000234         2979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTXY010000234 DAJTXY010000000
VERSION     DAJTXY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.238"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            243..1856
                     /gene="mppA"
                     /locus_tag="OO566_004149"
     CDS             243..1856
                     /gene="mppA"
                     /locus_tag="OO566_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6607181.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1907..2938)
                     /gene="ynaI"
                     /locus_tag="OO566_004150"
     CDS             complement(1907..2938)
                     /gene="ynaI"
                     /locus_tag="OO566_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6607182.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT          860 a          657 c          695 g          767 t
ORIGIN      
        1 acagatgctg attatgatac cgaaaactgg tttgaacgtg cgaagcccga acgtagtgtt
       61 cggtgttcta tgtgctttgc cgcattttgg cgtctattat ttacactaaa tctgatttga
      121 tatattgata cttaaaacat ttgatgcttc ctttgtcact tttttgatgg aagttgtttg
      181 catttcttta aggcgaaaca aataattacg catcaatttt aatgtcggtt agagggaaac
      241 ttatgaagca ctctgtttca gttacgtgtt gtgcgctgtt ggtcagcagc atttctcttt
      301 cgtatgctgc agaagttccg agcggcacag tactggcaga gaagcaggag ctggtgcgcc
      361 acattaaaga tgagcctgcg tcgctggatc ccgctaaagc cgtgggcctg ccagagattc
      421 aggtcattcg cgatctgttt gaaggtctgg tgaatcagaa cgaaaaaggg gagattgtcc
      481 ccggcgtcgc gactcaatgg aaaagtaatg acaaccgtat ctggactttt accctgcgcg
      541 ataacgcaaa atgggcggat ggcacaccgg taacggcgca agattttgtc tacagctggc
      601 aacgtctggt ggacccaaaa acattgtcgc catttgcatg gtttgccgcg ctggcgggaa
      661 tcaacaacgc acaggcgatt attgatggta aagctacgcc tgatcagctt ggcgtcaccg
      721 cagttgatgc ccatactttg aaaattcagc ttgataaacc gttgccgtgg tttgtgaatt
      781 taaccgctaa ctttgccttc ttcccggtgc aaaaagccaa cgtagaaagc ggtaaagagt
      841 ggacgaaacc cggaaatctg atcggcaatg gcgcttatgt tcttaaagag cgcgtagtca
      901 atgaaaaact ggtcgtggta ccgaataccc attattggga taacgccaaa acggtgctgc
      961 aaaaagtgac cttcctgcca attaatcagg aatccgcagc cactaagcgt tacctcgcgg
     1021 gggatattga tatcaccgaa tccttcccga aaaatatgta tcagaagctg ttgaaggata
     1081 ttccggggca ggtttatacg ccaccgcagc tcgggaccta ttattatgcc tttaacacgc
     1141 aaaaagggcc gacggcagat cagcgcgttc gcctggcatt aagtatgacg atagatcgcc
     1201 gcctgatgac cgaaaaagtg ttggggacgg gcgaaaagcc agcgtggcat tttataccag
     1261 atgttaccgc cggatttacg ccggaacctt cgccgtttga acaaatgagt caggaagaac
     1321 tgaatgcgca ggcaaaaact ttgttgagcg cagctggtta tggtccgcaa aaaccgctga
     1381 agctgacgct tttgtataac acttcagaaa accatcaaaa aattgcgatt gctgtagcat
     1441 cgatgtggaa aaagaacctt ggcgtagatg ttaaattgca aaatcaggaa tggaaaacct
     1501 atatcgatag ccgtaacacc ggcaattttg atgttatccg cgcctcatgg gtgggggatt
     1561 ataatgaacc ctccactttc ctgacattat taacgtcaat gcattcagga aatatttcac
     1621 gctttaacaa tccggcatac gacaaagttc tggcccaggc atcgacggaa aataccgtta
     1681 aagcgcgtaa tgccgattac aacgcggcag aaaaaatcct catggagcaa gcaccgattg
     1741 caccaattta tcaatatacc aatggacgat taatcaaacc gtggctgaaa ggttatccca
     1801 ttaataaccc tgaagatgta gcgtacagtc ggactatgta tattgtgaag cattgatgtg
     1861 atgggaactg gcgttaccct tgtggataac gccagtgatg tctgatttag cgaccctgtt
     1921 ccggcggtgt aatgttatcc atatacagcg tctggctggg gaaggcaaag tccgcgccgt
     1981 gtgactgtac aatatcgata atcttcaaat aaacgtcttg ctgtgcagca agccactcag
     2041 cccataccgt ggttttggta aagcaataaa ccataatatt caatgacgag tcagcaaact
     2101 ggttgaaata aaccagtaag gtttgtcgct ggtcgatggc cgggtgattt ttcagcatct
     2161 cacgtacggc ttcgacaata acgcccactt ttgccgcatc ctcataacgt aaaccaatgg
     2221 tcgtggtaat gcggcggttg gtcattcgtc ctgggttttc tacgctgatc gacgaaaaca
     2281 gcgagttcgg tacgtacaat ggacgattat caaaggtcgt aattttggta attcgccagc
     2341 caatttccgc tactgtacct tcgatatttc tgtccggtga acggatccag tcgccaatac
     2401 tgaaaggacg gtcgaaatag agcatgatcc cggaaaagaa attactcaga atatctttac
     2461 cggccatacc gacagccaga ccaccaatac caccaaaggt cagcaagcca gaaaggctca
     2521 tgccgaaatg ttcgccataa agcagaacaa gcactacaat aatggtgatt ttgataatgc
     2581 gcgacataat ccgcgcactg gtgatatcgc gacctttttt aatctgctgt ttttcaaact
     2641 gattaatcag cagaaatagc ttaatcgtca gaataaccgc aatcagggac gtacagataa
     2701 aatcgataac gcctggggtg ataaatttga gtttatagtt ttctataaca taattaatga
     2761 tgctaccaac agcactgata attatggtgt agattaaaaa ttgcaccgca tggaataaaa
     2821 atccttttct tttacgattt ccacggcgaa accaaaagct catcagaatc aatgctgcgc
     2881 agctaccgaa aataatgacc agattaagcg cattatttgt aaacagttca gcgatcattg
     2941 ttttatcagg ctcctccaga taattgtcgt catgccgga
//
LOCUS       DAJTXY010000235         2945 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTXY010000235 DAJTXY010000000
VERSION     DAJTXY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.239"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            133..2001
                     /gene="kup"
                     /locus_tag="OO566_004151"
     CDS             133..2001
                     /gene="kup"
                     /locus_tag="OO566_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6607183.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2168..2587
                     /gene="rbsD"
                     /locus_tag="OO566_004152"
     CDS             2168..2587
                     /gene="rbsD"
                     /locus_tag="OO566_004152"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6607184.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2595..>2945
                     /locus_tag="OO566_004153"
     CDS             2595..>2945
                     /locus_tag="OO566_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6607185.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKT"
BASE COUNT          656 a          703 c          772 g          814 t
ORIGIN      
        1 gctttttctt tgagccaggt taatgtggcc gcatttagga gtacgatttt gccgttaatc
       61 gtgcatactg tgcgcttttt tgtgggccaa gggactaagc acacatttca tatttcaacg
      121 aaagactagt ctatgagcac tgataataag caatcattgc ccgcgattac cctcgcggcg
      181 attggagttg tctacggcga tattggtacc agcccgttat atacacttcg tgaatgtttg
      241 tccggccagt ttggttttgg cgttgaacgc gatgccgtgt ttggcttttt atcgctgatc
      301 ttctggctgc taatctttgt ggtttccatt aaatatctca ccttcgtgat gcgggcagat
      361 aacgccggtg agggggggat cctgacgttg atgtcgcttg ccgggcgtaa tacgtcggcg
      421 cgaaccacat caatgctggt gattatgggg ctaatcggcg gcagcttttt ctatggtgaa
      481 gtcgtcataa cacccgctat ttcggtgatg tcagccattg aaggtctgga aatcgtcgcc
      541 ccgcagctgg atacctggat agttcccctc tcaattatcg ttctcacttt attatttatg
      601 attcaaaagc atggcacggc tatggtcggt aagctgtttg cgccgatcat gctgacctgg
      661 tttttgattc tggcaggact ggggttacgt agcattattg ctaacccgga agtgctgcat
      721 gcgctgaatc cgatgtgggc ggtgcatttc ttccttgaat acaaaacggt ttcttttatt
      781 gcattagggg cagtggtgct gtcgattacg ggggtcgagg cgctgtatgc tgatatgggg
      841 cactttggta agttccctat tcgcctggcg tggttcaccg tcgtattacc ttccttgacc
      901 cttaattact tcggtcaggg agcgttgttg ttaaagaacc cggaagcgat taagaacccg
      961 ttcttcctgt tggcaccgga ctgggcgctg atcccactgc tgatcatcgc cgcactggcg
     1021 acggtaattg cctcgcaggc ggttatctct ggcgtcttct cattgacgcg tcaggcggta
     1081 cgtctgggat atttgtcgcc gatgcgcatt attcacacct ccgaaatgga gtcagggcaa
     1141 atctatattc cgtttgtgaa ctggatgctc tatgtcgcgg tcgtgattgt gattgtcagc
     1201 tttgagcact ccagcaacct ggcggcggcg tacgggattg cggtgaccgg aaccatggta
     1261 ctgacgtcta ttctctcgac taccgtggca cgtcagaact ggcactggaa taagtatttt
     1321 gttgcgctga tcctgattgc tttcctttgt gtcgatattc cattgttcac cgctaacctc
     1381 gataaactgc tctccggcgg ctggttgcca ttgagcctcg gtactgtgat gtttatcgtg
     1441 atgaccacct ggaagagcga gcgtttccgc ttgctgcggc ggatgcatga acatggtaac
     1501 tctctggaag cgatgattgc ttcgctggag aaatcaccgc ctgttcgcgt gcccgggacc
     1561 gcggtgtata tgtcgcgtgc aatcaacgtc attccctttg cgctgatgca taaccttaaa
     1621 cataacaagg tattgcatga gcgggtgatt ttgttaactc tgcgcaccga agacgcgcca
     1681 tatgtccata acgtccgtcg ggtacagatt gaacaactgt cgcccacttt ctggcgcgtg
     1741 gtggcaagtt atggttggcg agaaacgcca aacgtggaag aagttttcca ccgctgcggt
     1801 ctggaaggat taagttgccg gatgatggaa acctccttct ttatgtcgca tgagtcgttg
     1861 atcctcggca aacgcccgtg gtatttgcgt ctgcgcggca agctgtactt gctgctgcaa
     1921 cgtaatgcgc tgcgtgcacc agatcaattt gaaatcccgc caaacagggt tatcgaactg
     1981 ggtactcagg tcgaaatcta acgccagacg cctcctttct tcataagggg gcgtttttgt
     2041 tttcatggtt aatcaccatg taaaacgttt cgaggttgat cacatttccg taacgtcacg
     2101 atggttttcc caactcagtc aggattaagc tgcggatcag cgaaacgttt cgctgatgga
     2161 gaaaaaaatg aaaaaaggca ccgttcttaa ttctgatatt tcatcggtga tctcccgtct
     2221 gggacatacc gatacgctgg tggtgtgtga tgctggttta cccatcccca aaagtacaac
     2281 gcgtatcgat atggcattaa cccaaggtgt accttctttt atgcaggtgc tgggcgtcgt
     2341 cacaaatgaa atgcaggtcg aggctgccat tatcgcggaa gagatcaaac aacataatcc
     2401 gcaactccac gaaacgttgc tcactcacct tgagcagctg caaaaacacc agggaaatac
     2461 cattgaaatt cgttacacca cgcatgaaca attcaaacaa caaaccgcag aaagtcaggc
     2521 ggtaattcgc agcggagaat gttctccgta tgcgaatatc attctctgtg ctggcgtgac
     2581 gttctgaggc cgtcatggaa gcattacttc agcttaaagg catcgataaa gccttcccgg
     2641 gtgtaaaagc cctctcgggc gcagcgttaa atgtctatcc gggccgtgtg atggcgctgg
     2701 tgggcgaaaa cggcgcgggt aaatccacca tgatgaaagt gcttactggt atctatactc
     2761 gcgatgccgg cacgctttta tggctgggga aagaaacgac atttaccggg ccgaaatctt
     2821 cccaggaagc cgggattggg attatccatc aggaactgaa cctgatcccg cagttgacca
     2881 ttgccgaaaa cattttcctc ggtcgtgagt ttgttaatcg ctttggcaaa attgactgga
     2941 aaacc
//
LOCUS       DAJTXY010000236         2853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTXY010000236 DAJTXY010000000
VERSION     DAJTXY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.240"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..452)
                     /gene="intS"
                     /locus_tag="OO566_004154"
     CDS             complement(<1..452)
                     /gene="intS"
                     /locus_tag="OO566_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6607186.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDR"
     gene            complement(614..688)
                     /locus_tag="OO566_004155"
     tRNA            complement(614..688)
                     /locus_tag="OO566_004155"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(653..655),aa:Arg,seq:cct)
     gene            complement(764..1696)
                     /locus_tag="OO566_004156"
     CDS             complement(764..1696)
                     /locus_tag="OO566_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6607187.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMVIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1990..2745
                     /gene="mlaA"
                     /locus_tag="OO566_004157"
     CDS             1990..2745
                     /gene="mlaA"
                     /locus_tag="OO566_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6607188.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPVRNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          688 a          693 c          686 g          786 t
ORIGIN      
        1 cgatcttcaa atctgcggat tacttccagc agttgcatcg gctcaatatc ctgaatctca
       61 aggccgccga tgatgggtaa aatgtcgtca tcaaacattt ttgcaagttc agtcgcatac
      121 cctactgacc agacttgctt cttgtgttcg taccattcct tgtaaatcgc actaaatgaa
      181 ttgttgttag acgaagcctt tttcgccttt accggatcga tgccagccga gatgtctttc
      241 ctcgcagtcc atgctttatc ccttgcctcc tgcaaagtca taagcggata ttttccgacg
      301 gtcaggattt tctccttacc gtcaatcttg tagcgaagct gccatacctt tttcccggat
      361 acagggacat aaaggtacag gccattacca tcgagaaggc ggtatggttt ttctttcggc
      421 tttgctgctt caatctgctt aacggtgagc atgggtaaaa atccggtggg taaaattatt
      481 ttatccactt tttacccgtc atggagtgcg gctgtcaacg atctgacgcg aaccatgacg
      541 aactgtgaat atacggaagg cttgatattc aggggatttt gcggactggt acggatggga
      601 gcgaactgat aaatggtgtc ccctgcagga atcgaacctg caattagccc ttaggagggg
      661 ctcgttatat ccatttaact aagaggacaa tgcggcatga gtatacccgc taatggactg
      721 cggggtaagt acgctgccgc tcgattgctt aaaccctcgc catttatgct gggtttttat
      781 catttttctt aatgttttcc gcacgttctg ctttttggcg tgcttctgct ttacgcttgt
      841 tgctcatgtc gttacgaatc tgtgcatgac tcattaacgc gaagataaag gtgccgccgc
      901 agatgttccc cgctaaagta ggtagtgcga agggccagat gaaatcgctc cagtgcagcg
      961 taccgttaaa caccagatag aggatttcaa cagaaccgac cacgatatgg gtggtgtcac
     1021 ccagggcaat aagccaggtc atcaatataa tcaccacaat ctttgccgca cctgctgcag
     1081 gaaacatcca taccatagtg gcgatcagcc agccggaaat gatcgcgttg gcaaacatct
     1141 cgctgggggt gttcttcatc acatccatgc cgattttgac aaatgcatcg cgagtttctt
     1201 cattgaagat aggcatatat tcaaatgccc acgcagcaat accagtcccg agaatattac
     1261 ccagcagcac gactccccat aaccgcataa gtaagccaac gttgctcatt gtcggttttt
     1321 gcatgacggg tagtaccgca gtcacggtgt tttcggtaaa taattgctgg cgggccatta
     1381 tgacgataat aaaaccaaag gtataaccga gattctccag caagaagctg cccggcacac
     1441 cttccagttc gacatgaaat atcccttttg ccagtaacga agcgcccatc gacagacccg
     1501 ccgcaatggc tgaccacagt agcgccattg cgtcgcgttc tagctctttt tcaccatcct
     1561 ggcggatatg ctcatgaatt accatcgctc gggaggggag tcggtcttca tctatttcta
     1621 tttttttgcc gcgctctttt tcttcgctct caacttcaat ttcgtcgctg tgttgatcaa
     1681 ttttgtcgtt gtccatggtc tcttctcttg aattagcacg tatagctaaa gcgtagcggc
     1741 ttttttgctc gcaactggcg ggagttactc tgaaaatgta gaaaaggttg cgtttgcctt
     1801 tttctgtttc tatagaatca agtagcctac agggcggcga ttaccaggct atgatcaaat
     1861 cagcaaatca gggcgtctgg acatcagttg acgtgctgtt acaatcgccc acacctaaac
     1921 aggcggatac ggtatcgttc cgtcatggat ggcaaactgc ataagccata aaaaaacagg
     1981 gagacattta tgaagcttcg cctgtcggcg cttgctctgg gaactacgct tctggtgggg
     2041 tgtgcgagtt ccggtacaga tcagcaaggg cgttctgacc cgttagaagg gttcaaccgc
     2101 accatgtaca acttcaactt caacgtatta gacccgtata ttgttcgacc ggtcgctgtc
     2161 gcctggcgtg attatgttcc gcaaccggtg cgtaatggtt tgagcaactt tactggcaac
     2221 cttgaagaac ctgcggtgat ggttaactac ttcttgcagg gcgaccctta tcaggggatg
     2281 gtccacttta cccgcttttt cctgaacacc attttgggga tgggcggctt tattgatgtt
     2341 gcagggatgg cgaacccgaa actgcaacgg actgaacctc accgcttcgg tagtacgctt
     2401 ggtcattatg gcgtgggtta tgggccttac gttcagttac cgttctacgg tagcttcacg
     2461 ctgcgtgatg acggtggtga tatggcggat ggtctttacc cggttctttc ctggctgacc
     2521 tggccgatgt ctgtgggtaa atggacgctt gaagggatcg aaacccgcgc tcagctgctg
     2581 gattccgatg gtctgctgcg tcagtcgtcc gatccttata ttatggtgcg cgaagcgtac
     2641 ttccagcgtc atgatttcat cgctaatggc ggcgaactca aaccgcagga aaatccgaac
     2701 gcgcaagcga ttcaggatga tttaaaagat atcgattctg aataagaaac aaataaaaaa
     2761 ggtgagtcgc aaaactcacc ttttttgttg ttatccctca attaccacaa gtaaaaggag
     2821 ataaaccgac aatgacccgg acgtaatgat gta
//
LOCUS       DAJTXY010000237         2559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTXY010000237 DAJTXY010000000
VERSION     DAJTXY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.242"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            206..1633
                     /locus_tag="OO566_004158"
     CDS             206..1633
                     /locus_tag="OO566_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR6607189.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFRFQNGVAIAFKNIQAGTARKF
                     TLSSANNSTKNAAFYLWGNPSRPVVAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLN
                     YGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSA
                     DNVDGVYYRPLQKLINGTWYNVASI"
     gene            1636..2180
                     /locus_tag="OO566_004159"
                     /pseudo
     CDS             1636..2180
                     /locus_tag="OO566_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2204..>2559
                     /locus_tag="OO566_004160"
     CDS             2204..>2559
                     /locus_tag="OO566_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6607190.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGA"
BASE COUNT          701 a          584 c          707 g          567 t
ORIGIN      
        1 ggcatcatct gcgtctgctt caaaagatga ggcgactaaa caggcgtcag cagcgaaggg
       61 cagtgccacg acggcatcct ctaaggcgac agagtctgca ggcagtgcga cggcggcagc
      121 gcagagcaaa agcgcggcag aatccgcggc aatgcgcgct gagacagcag caaaacgggc
      181 agaggatatt gctgatgtcg tatctctgga agatgcgagc acgacgaaaa aggggatagt
      241 gcagctcagc agtgcgacca acagtgcatc cgagtcactg gccgcgacag cgaaagcggt
      301 taaggtggta atggatgaaa caaacaagaa agcgcccctg aacagtccgg ctctgaccgg
      361 aacgccaacc acacccactg cgccaaaggg gactaataat actcagatcg ccagtacggc
      421 ttatgttatg gccgcgattg ctgccctcgt ggactcgtcg cctgatgcac tgaacacgct
      481 gaatgaactg gcggcggcgc tgggcaacga cccgaatttt gcgaccacca tgactaacgc
      541 gcttgcgggt aagcaaccga aagatgccac tctgacggcg ctggcggggc ttgctactgc
      601 ggcagacagg tttccgtatt ttacggggaa tgatgtcgcc agcctggcaa ccctgacaaa
      661 agtcgggcgg gatattcttg cgaaatcgac cgttgccgcc gttatcgaat acctcggttt
      721 acaggaaacg gtaaacaagg ctgataacgc tgtccaaaag acaggcgata ccttgtccgg
      781 tggacttact tttgaaaacg actcaatcct tgcctggatt cggaatactg actgggcgaa
      841 gattggattt aaaaatgatt cggatgcaga caccgattcc tacatgtggt ttgaaacagg
      901 tgacaacggc aatgagtatt tcaaatggag acaccgcctc gctggcggcc agctcaaaga
      961 gctgatgaat cttaaatggg attcactaaa tattctggtt aatgccgtca ttaatggttg
     1021 ccttggcatt ggtacgacga atgcgttagg tggaaattca attgctttcg gggataacga
     1081 taccggcctg aaacagaacg gcgatggtct gctggatgtt tatgcgaatg gacagcatgt
     1141 atttcgtttt cagaatggcg tggctattgc ttttaagaat attcaggccg gaactgccag
     1201 aaaattcacg ttatccagcg ccaacaactc cacgaaaaat gcagcgtttt atttgtgggg
     1261 taatccatcc aggcctgttg ttgcagagct tggcgatgat tcaggctggc attttttcag
     1321 ccagagaaac ccggataaca gcatagtgtt cactgttaac ggacaggtaa ttccgttaaa
     1381 ttacggaaac ttcgatgccc gctataaata tcgaacagag ggggtacagg atgtacggta
     1441 tggccatgaa atgtattaca gccccggcag taacaccgtt tcgtggagat tttgcgcacc
     1501 ttcgggacac gggctgtcag ggatggcgat atcggatacc ggccgtaact cagcggataa
     1561 cgttgacggt gtgtattacc gaccactgca aaaactgatt aatggcacct ggtataacgt
     1621 agcgagtatt taacaatgtt gcatttaaaa aatattactg cgggtaatcc gaaaacagca
     1681 gaacaatatc agatgacaaa acgatattcg gtcacctggc ttttttcaga agacggaaaa
     1741 aactggtatg aagagctgaa gaatttcgcc agcgacacaa taaaaatagc ttacaccgga
     1801 gatggtcgcg tggtgtgggt cggtaaggat gtgacaggca tcgagccacg caatgccagc
     1861 gttatcgaag ttccggatat taccgctaac cgacggatta ccgcccgggt tactggtttt
     1921 accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag cgtgatgccc
     1981 ttctggctca ggtcagtgcc cggacagggg aatgggaaga agacctgctg ctggggttaa
     2041 tcagcgacga agataaagaa aagctgaaag cctgtcgtat ttacgcgaaa tcgctgcagg
     2101 cgatggattt cagcaccatc actgataaat cctcatacaa cgccattgaa tggcccgcct
     2161 ctccggaagg ttcttcctga tttaatttat cgcgagaaaa acaatgtctg tagtgatatc
     2221 aggtgcgctg actgatggcg caggtatccc catgtccgga taccatatta ttctgaaatc
     2281 ccgggtaaac accccggaag tggtgatgaa cactgttgct gatgtgatga caggaaacga
     2341 tggtgaatac tgtttccatg cgcggactgg aaaatatggt gtgtatctga aacaggactg
     2401 gcgcaacgag tacaacgttg gcgacattgc tgtatatgag gactcaaagc ccggcacgct
     2461 gaatgacttt ctgattgctc ctgatgaggg cgacctgaaa ccggatgtcg tcaaacgctt
     2521 tgaggaaatg gtggcgcagg cgcagcagag cgccggggc
//
LOCUS       DAJTXY010000238         2458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTXY010000238 DAJTXY010000000
VERSION     DAJTXY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.243"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..354
                     /locus_tag="OO566_004161"
     CDS             <1..354
                     /locus_tag="OO566_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="required for the translocation of capsular
                     polysaccharide through the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6607191.1"
                     /translation="VGKQSTLKMDRSGMTLAEALGNAEGMNQDVADATGIFVIRATQN
                     KQNGKIANIYQLNAKDASAMILGTEFQLEPYDIVYVTTAPLARWNRVISLLVPTISGV
                     HDLTETSRWIQTWPN"
     gene            342..788
                     /gene="etp"
                     /locus_tag="OO566_004162"
                     /gene_synonym="yccY"
     CDS             342..788
                     /gene="etp"
                     /locus_tag="OO566_004162"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6607192.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            808..>2458
                     /locus_tag="OO566_004163"
     CDS             808..>2458
                     /locus_tag="OO566_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine kinase"
                     /protein_id="HCR6607193.1"
                     /translation="MTTKNMNTPLGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMLPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINTLQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQHQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSS"
BASE COUNT          656 a          587 c          653 g          562 t
ORIGIN      
        1 gtgggtaaac agagcacgct gaaaatggac cgcagcggta tgaccctggc agaagcgctg
       61 ggtaacgctg agggtatgaa tcaggatgtc gctgatgcga cggggatttt tgttattcgt
      121 gcaacgcaaa acaagcagaa tggcaaaatc gccaatattt atcagctgaa tgcgaaggac
      181 gcatcggcaa tgatactcgg aacggaattc cagcttgaac cttacgacat tgtttatgtc
      241 actaccgcgc cgctggctcg ttggaaccgt gtgatttctc tgctggtacc gacgatttcc
      301 ggggtgcatg acctgacaga gacttcacgg tggattcaaa catggcccaa ctaaaattta
      361 actcaatcct agtggtttgt accggcaaca tttgccgttc gcccattggc gagcgcttgc
      421 tgcgtaaacg tctgccgggc gtaaaagtca aatcggcagg tgttcatggt ctggtaaaac
      481 accctgcaga tgcgacagcg gcagatgttg ccgctaatca tggcgtatct cttgaaggac
      541 atgctggacg taagctcact gctgagatgg cacgaaatta cgatctgatt ctggcaatgg
      601 agtcggaaca tattgctcag gttaccgcaa ttgctcccga ggtccgcggg aagacaatgc
      661 tgtttgggca atggctggaa cagaaagaga tcccggatcc ctatcgtaaa agtcaggacg
      721 catttgaaca tgtctacggt atgttggagc gcgccagtca ggaatgggcg aagcgcctca
      781 gccggtaatt tgagtttata aaatacgatg acaactaaaa atatgaatac gccactaggc
      841 agcactcagg aaaatgagat cgatctgctt cgtctggtcg gcgagttatg ggatcaccgt
      901 aagtttatta tcagcgtgac cgcgttattc acgctgatcg ctgtcgctta ctcgctgtta
      961 agcacaccaa tttatcaggc agatactctg gtccaggttg agcaaaaaca gggcaacgcc
     1021 attctcagcg gcctgagcga tatgctccct aactcatcgc ccgagtctgc accggagatc
     1081 caactgctgc aatcgcgcat gattctcggt aaaaccattg ctgaactgaa tctgcgcgac
     1141 atagttgagc agaagtattt tccgattgtg ggtcgcggct gggcgagatt aaccaaagaa
     1201 aaaccaggtg agctggcgat cagctggatg catattccac aactgaatgg tcaggatcag
     1261 caactgacac tcacggttgg ggaaaacggc cactatacac tggaaggtga agagttcacc
     1321 gtcaatggta tggtcggcca gcgtctggaa aaagatggcg ttgcgctgac tatcgcggac
     1381 attaaggcca aaccaggaac acagtttgtc ctgagccagc gtaccgaact ggaagcgatt
     1441 aacacattgc aggaaacctt taccgttagc gaacgcagta aagaaagcgg gatgctggaa
     1501 cttaccatga ctggtgatga tccccagttg attactcgta ttctgaacag catcgctaac
     1561 aactatttgc aacagaatat cgctcgccag gcggcgcagg attcacaaag ccttgaattc
     1621 ttacagcacc agttacctga agtgcgcagc gagctggacc aggcggaaga aaaactcaac
     1681 gtttatcgcc agcagcgcga ttcggttgac cttaacctgg aagccaaagc cgttcttgag
     1741 cagattgtga acgttgataa tcaactcaat gagctgacct tccgcgaggc agagatctcc
     1801 cagctgtata agaaagatca cccaacttat cgtgcgctgc tggaaaaacg ccagacgctg
     1861 gagcaagaac gcaaacgcct gaataagcgg gtatcggcaa tgccttccac ccaacaggaa
     1921 gtgttgcgtt taagtcgtga cgtagaagcg ggccgtgcgg tatatctgca attacttaac
     1981 cgccagcagg agttgagtat ttcgaaatcc agtgccattg gtaacgtgcg gattatcgac
     2041 ccggcagtca ctcagccgca gccagtgaaa ccgaaaaaag cgttgaatgt ggtgcttggt
     2101 tttattcttg gcctgtttat ttcggtgagt gccgtgctgg cgcgtgcgat gttgcgtcgt
     2161 ggtgtagaag ccccggaaca actggaagag cacggcatca gcgtttattc cactatccca
     2221 atgtccgagt ggctggataa acgcacccgt ctgcgtaaga aaaatttatt ttctaatcag
     2281 cagcgccatc gtactaaaaa tatccccttc ctggcggtgg ataacccggc ggattctgct
     2341 gtggaagccg tacgtgcgct acgaaccagt ctgcatttcg ctatgatgga gactgagaat
     2401 aacattctga tgatcaccgg tgcgacgcca gacagtggta aaacgtttgt cagttcaa
//
LOCUS       DAJTXY010000239         2346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTXY010000239 DAJTXY010000000
VERSION     DAJTXY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.244"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..158
                     /locus_tag="OO566_004164"
     CDS             <1..158
                     /locus_tag="OO566_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR6607194.1"
                     /translation="STPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRMKER
                     RKEWGIL"
     gene            143..781
                     /locus_tag="OO566_004165"
     CDS             143..781
                     /locus_tag="OO566_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6607195.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1009..1410
                     /locus_tag="OO566_004166"
     CDS             1009..1410
                     /locus_tag="OO566_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607196.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEISLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSSGGGR"
     gene            1421..1895
                     /locus_tag="OO566_004167"
                     /pseudo
     CDS             1421..1895
                     /locus_tag="OO566_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1984..>2346
                     /locus_tag="OO566_004168"
     CDS             1984..>2346
                     /locus_tag="OO566_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR6607197.1"
                     /translation="MVHKSDSDELSALRAENARIIKPLLPPEPATPRAGRPWAEHRKI
                     INGMFWVLCSGAPWRDLPERYGAWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFID
                     WSATALDGSNIRALKCAAG"
BASE COUNT          690 a          442 c          556 g          658 t
ORIGIN      
        1 ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag aatgattttt
       61 ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt tacctgcagc
      121 gtatgaagga aaggaggaag gaatggggga ttctgtgaca ccggagatac ttggcaacat
      181 gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg ctctgaatca
      241 tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg actgtgtgtt
      301 gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca tggcactagg
      361 tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac cggtggtggt
      421 gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt tactggggcg
      481 ggaagcagat acaggaaaac gcctgagggg ctggttgcct gtagcctgta ttaatccgga
      541 gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg ccagggggaa
      601 acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg gctggacgaa
      661 tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc tgaaacagat
      721 tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga cggttcgttg
      781 atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa aaaatgatac
      841 cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt aggctttgat
      901 aacgggataa aatgagcggc ttttctcata ccatcaggtg tgatgatggc gcagatttac
      961 aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat gaaaaaacag
     1021 atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc gaaaatattt
     1081 aaatacattg aaatctctct gccgtttttc tactttatct atacatcagg cgaaccattc
     1141 cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat atttattaat
     1201 aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca aagaagagat
     1261 atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt atttgatcat
     1321 gacattgaac gattaaaaaa aatttccaaa gctcagctta tatctcccga ttatgttttg
     1381 attgattttt ctagtggggg ggggagatga accctatgca atgtcctggt taatatctca
     1441 gtgtgctcat cagtgcactg ataataaaaa gacggagaca gatgccattt acgataaagt
     1501 tcggagttct tatttgcttt catgtattct gaaaaaaaac aaaaatgttg ggcttattct
     1561 tcatgcacca tcatttgttt ctgtgtctga aaaaaatcgc cagaattgtg atggcaaatt
     1621 actcccgtaa ctggagtaat tccgagttgg ccagtgcggt tttaatgagt gaatcttctt
     1681 taaaaagaag aatgtacaaa gaggtcggtt ctataagtac atttgtacat aaaataaagc
     1741 ttacagaagc tatcaggaaa ttacgaagga caaatactcc catttctgtc atatcatctg
     1801 agttaggtta cagttcacca tcgtattttt ctaaagtttt ctttaaatat ttaaagacat
     1861 accctcaaaa cataaggaag aaaaatggtc ggtaatgtca ccaattagga ggacttgcag
     1921 agccgctttt ttacgatact gagatcaaac agacataatc tatgcggaga tttacagcag
     1981 ggcatggttc acaaatctga cagcgatgaa ttatcggcac tgcgggcaga aaatgctcga
     2041 atcatcaagc ccctgttacc tcctgaaccc gccacaccac gggccggacg cccatgggct
     2101 gagcaccgta aaatcatcaa tggcatgttc tgggtgttat gttccggtgc accatggcgc
     2161 gatttacccg aacgatatgg ggcatggaaa actgtttata accgctttaa ccgatggtca
     2221 aagtcaggcg tgattaatat tattttcaac aggttgcttt cgctacttga tgcaaacggc
     2281 tttattgact ggtctgccac cgcgctggat ggcagtaata tccgggcgct gaaatgtgcc
     2341 gccggt
//
LOCUS       DAJTXY010000240         2280 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTXY010000240 DAJTXY010000000
VERSION     DAJTXY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.245"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="OO566_004169"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="OO566_004169"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6607198.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          578 a          564 c          671 g          467 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacgctt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagtc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgctatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc ttactctact cagcacatgg
//
LOCUS       DAJTXY010000241         2231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTXY010000241 DAJTXY010000000
VERSION     DAJTXY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.246"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(155..667)
                     /locus_tag="OO566_004170"
     CDS             complement(155..667)
                     /locus_tag="OO566_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6607199.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="OO566_004171"
                     /pseudo
     CDS             862..1626
                     /locus_tag="OO566_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1577..>2231
                     /locus_tag="OO566_004172"
     CDS             1577..>2231
                     /locus_tag="OO566_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6607200.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     "
BASE COUNT          639 a          486 c          562 g          544 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatgtaa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg ctcgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca acgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggactagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat g
//
LOCUS       DAJTXY010000242         2210 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTXY010000242 DAJTXY010000000
VERSION     DAJTXY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.247"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..246)
                     /locus_tag="OO566_004173"
     CDS             complement(<1..246)
                     /locus_tag="OO566_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607201.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFC"
     gene            271..510
                     /gene="relB"
                     /locus_tag="OO566_004174"
     CDS             271..510
                     /gene="relB"
                     /locus_tag="OO566_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR6607202.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            510..797
                     /gene="relE"
                     /locus_tag="OO566_004175"
     CDS             510..797
                     /gene="relE"
                     /locus_tag="OO566_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR6607203.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            869..1024
                     /gene="hokD"
                     /locus_tag="OO566_004176"
     CDS             869..1024
                     /gene="hokD"
                     /locus_tag="OO566_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6607204.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1242..1492
                     /locus_tag="OO566_004177"
                     /pseudo
     CDS             1242..1492
                     /locus_tag="OO566_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1559..1837
                     /locus_tag="OO566_004178"
     CDS             1559..1837
                     /locus_tag="OO566_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607205.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            1839..>2210
                     /locus_tag="OO566_004179"
     CDS             1839..>2210
                     /locus_tag="OO566_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607206.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQ"
BASE COUNT          569 a          511 c          576 g          554 t
ORIGIN      
        1 gcaaaaaata gatgaataag ttgagttgtg catatgtagc ctgaccgtca caaagtatat
       61 ggtgtctgta ccagtaagat gatggccgga ctctttaaaa aagagctgac ctgcacaata
      121 caggatggac ttagcaatgg ctgctcctgg cacaaagcgg acagtgatca ccgttcttac
      181 gactactttc tgacttcctt cgtgacttgc cctaagcatg ttgtagtgcg atacttgtaa
      241 tgacatttgt aattacaaga ggtgtaagac atgggtagca ttaacctgcg tattgacgat
      301 gaacttaaag cgcgttctta cgccgcgctt gaaaaaatgg gtgtaactcc ttctgaagcg
      361 cttcgtctca tgctcgagta tatcgctgac aatgaacgct tgccgttcaa acagacactc
      421 ctgagtgatg aagatgctga acttgtggag atagtgaaag aacggcttcg taatcctaag
      481 ccagtacgtg tgacgctgga tgaactctga tggcgtattt tctggatttt gacgagcggg
      541 cactaaagga atggcgaaag ctgggctcga cggtacgtga acagttgaaa aagaagctgg
      601 ttgaagtact tgagtcaccc cggattgaag caaacaagct ccgtggtatg cctgattgtt
      661 acaagattaa gctccggtct tcaggctatc gccttgtata ccaggttata gacgagaaag
      721 ttgtcgtttt cgtgatttct gttgggaaaa gagaacgctc ggaagtatat agcgaggcgg
      781 tcaaacgcat tctctgaacc aaagcatgac atctctgttt cgcaccgaag gtgacacttc
      841 tgctttgcgt tgacaggaga agcaggctat gaagcagcaa aaggcgatgt taatcgccct
      901 gatcgtcatc tgtttaaccg tcatagtgac ggcactggta acgaggaaag acctctgcga
      961 ggtacgaatc cgaaccggcc agacggaggt cgctgtcttc acagcttacg aacctgagga
     1021 gtaagagacc cggcgagtga gaaatccctc gccacctctg atgtggcagg catcctcaac
     1081 gcacccgcac ttaacccgct tcggcgggtt tttgttttta ttttcaacgc atttgaagtt
     1141 ctggatggtg ccggaataga atcaaaaata cttaagtagc gcgcagggat aaaagggatg
     1201 gaccccgaac aggggagtgc tacttatctg gaaggattct gatgatgaac atcgaagaac
     1261 tgcgtaaaat tttttgtgaa gatggcctct atgctgtgtg cgttgaaaat ggaaatattg
     1321 ttagtcatta ccgcattgtg tgtttgcaaa aaatggggct gcgttaatta attttgtgga
     1381 tgcccgagtg acggacggat ttatcttgcg cgacggtgag tttgtcactt cattacaggc
     1441 attgaaagag atcggaataa aagctggctt ttctgctttt tcagaagaat aaactcatct
     1501 acaatcttgc gcggggctga actcccgctg agtaacaccg tgccaccgga gaaaaccgat
     1561 ggcacgcaac gcaaaatatt acaatcatga taattcgacc gttcttgccc acacgcacga
     1621 gcggtattct cacgcattta agtcagactg gtaccagcat ccctcatgca ctgaagaaca
     1681 ggccgaatgg ctcattcagt gttaccgcag gcgcggatgc gaggttaaaa aagcccttag
     1741 cctcgactac cgtcactgga taatctccgt caggctccct tactccgaac agccaccgcg
     1801 tccgtcccgc acattccagc aacggatctg gaggtaacgt gcgggtatta cttcgacctg
     1861 ttctggttcc ggaactcggg ctggtggtcc ttaagcccgg tcgtgaatcc atgccggtat
     1921 tccacaatac ccggttactg gtggagccgg aaccgaaaag catgcgtaat ctgccgtccg
     1981 gggtcgttcc tgccgctcgc cagccgctgg tggaagacaa aacattgctg ccgtttttca
     2041 gtaacgcacg ggtgattcgt gctgctggtg gtgctggtgc attgtctgac tggctgttgc
     2101 gccatattaa atcctgccag tggccacacg gcgattatca tcacagcgaa accgtcattc
     2161 accgttatgg taccggcgca atggtgttgt gctggcactg cgacaaccag
//
LOCUS       DAJTXY010000243         2204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTXY010000243 DAJTXY010000000
VERSION     DAJTXY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.248"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..606
                     /locus_tag="OO566_004180"
     CDS             <1..606
                     /locus_tag="OO566_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6607207.1"
                     /translation="AKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAM
                     VFNGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            638..1246
                     /locus_tag="OO566_004181"
     CDS             638..1246
                     /locus_tag="OO566_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607208.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKSVLAVNSEQFRGLTVERLAKYIEMA
                     KHIDHEISDNGYRPSEGYLPAAERRSLMLAFGVSGVSMWPSA"
BASE COUNT          611 a          487 c          543 g          563 t
ORIGIN      
        1 gctaaagcgt actcgcagaa gacctcaggt aagccatacc attattacgt caccgctctg
       61 gctgcgatta caggtgcgag acttaacgag gtggcccaac ttcaggttaa agatgtcagg
      121 accactgagg ctgggaccgt ttttatccat atcaacgaag acgatagcag cctgccgggt
      181 aagagtatca agaacgccca cagtgaccgc tgtgtcccgt tggtagatgg tgcttatggt
      241 tttgtattgg ctgactttat gtcactcgtg gaagaccgta gaaagactga gggtgataat
      301 gctatggtgt tcaatggcct caagctaatg aagaacggct acggtgagca ggttagcaag
      361 tggtttaacc gaacgttgct gcccaaagtc ctcgctgacc gtagcggctt agcttttcac
      421 tcgtttagac atacagttgc gacccagtta aaacaacatg gggtcgagtt agcgtatgct
      481 caggcgatta tgggtcatag ctcagggtct ataacttacg accgttacgc gaaagaggtc
      541 gaggtggaaa cgctgaaaga aaaattagcg gagtcattgt cagtaaaaaa aatagacgga
      601 aagtaatcgc atagggatag cggaatgtga ggacgtaatg gtcaaagaat ttttagttag
      661 caaagaaaat aagccttcat atgaatgtat gaatactgct gaaacttgtt tggggctggc
      721 tggaatggct tcggaagatt ggcaacgtga gcatcattgc cgcacaggta taatatttac
      781 tgcttttgct attgaggcaa tgttcatctt ctatagaaaa caggttgacc ccggctacga
      841 taagacgcag aaagagtgca gaaagacaat gcacaagaat acgttaaaat tgtgcggtat
      901 aaacaattat atggggacaa aaccttacca aattatcaaa gagtgtcttg aggtaagaga
      961 cgctattgct cacggcgatt cgtatacttc gagctttaat ttctcggcgg atcatttgga
     1021 taaccaagac gatattgcaa aaagtgtcct ggcggtaaac tctgaacaat ttcggggatt
     1081 aactgttgag cgccttgcaa aatacattga aatggcaaag catattgacc atgagatttc
     1141 tgataatggt tacaggcctt ctgaaggtta ccttccagca gccgagcgca gaagtctgat
     1201 gttagctttt ggcgtcagtg gtgtgtcaat gtggcccagt gcttgagcga taatacacct
     1261 cactatagag gaatggaccc ttaactatca ctatagggga gagaccctaa gtccccacct
     1321 taatagcagt cacgacccat acacacattc attaagtctc aggcttccgg tagcggaccg
     1381 attggaaacc atccgcagac gcctagtgtt aaatcacctt ggcctgagac cattcctttt
     1441 aacgtaagct atcttccctt ttgacaggcg gggagcagta gaccacatcc gactttaagt
     1501 tggctctgtg ataatcgctg cgcggataac tgaagaacca agacaccatt actgaggtct
     1561 gccatccatt tgtggacagg cggaaactca aagaggttag atcagtggtg tctgaggttg
     1621 gagctttgag tcttgaccag caggctggag gagaccatga gtttatcttg ttatggattg
     1681 tgttatcaca attccaataa gaagaacttt aagtccagcc tccactcccg ccaacccgaa
     1741 gtaaaattat ggtcgctatg atgtggtctc catgcttcgg gtcttactgg aagtactgaa
     1801 cactttcgcg tcaagttctc cgaagttcat tcctgtttga ctaaactatc actataggga
     1861 ccatatctcg cctctgacct cctgagagac ggcaagcgag aaaccgtggt ccacgctagc
     1921 aagctagcca ccagcctaag gcaagacagc ctctggtcag cctgtgtgtt ctttggtctc
     1981 gtgacttaag tctatcacca cacacgctcg gacatgctgt cgaaccttca tctctatggt
     2041 catcgcagac cactaacccc aattacctca agtcattctg tttgcgtcct gttgcctgct
     2101 ggtctccctt tacgtggggg cttgagtagg tcagtggggt gcgacttcag ggtgcaaaca
     2161 aaaggagtac acgtaaatgt cccaacaaga aatcagcgtc atta
//
LOCUS       DAJTXY010000244         2188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTXY010000244 DAJTXY010000000
VERSION     DAJTXY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.249"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..>2188
                     /locus_tag="OO566_004182"
     rRNA            1..>2188
                     /locus_tag="OO566_004182"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          601 a          478 c          673 g          436 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc acatactgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag
      481 aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc agtgggagca
      541 cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat attctgtagc
      601 aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg ttaagttgca
      661 gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg ggtaacacta
      721 actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg ctgggggtga
      781 aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg tagcgcctcg
      841 tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg acttaccaac
      901 ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga gacacacggc gggtgctaac
      961 gtccgtcgtg aagagggaaa caacccagac cgccagctaa ggtcccaaag tcatggttaa
     1021 gtgggaaacg atgtgggaag gcccagacag ccaggatgtt ggcttagaag cagccatcat
     1081 ttaaagaaag cgtaatagct cactggtcga gtcggcctgc gcggaagatg taacggggct
     1141 aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt gttgggtagg ggagcgttct
     1201 gtaagcctgt gaaggtgtac tgtgaggtat gctggaggta tcagaagtgc gaatgctgac
     1261 ataagtaacg ataaagcggg tgaaaagccc gctcgccgga agaccaaggg ttcctgtcca
     1321 acgttaatcg gggcagggtg agtcgacccc taaggcgagg ccgaaaggcg tagtcgatgg
     1381 gaaacaggtt aatattcctg tacttggtgt tactgcgaag gggggacgga gaaggctatg
     1441 ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag gcaaatccgg
     1501 aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc tgaagcaaca aatgccctgc
     1561 ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac cgacacaggt
     1621 ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact aggcaaaatg
     1681 gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg aagcgacttg ctcgtggagc
     1741 tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca
     1801 aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg
     1861 ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt aactataacg
     1921 gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga
     1981 tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt
     2041 acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac tgaacattga
     2101 gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg acgccagtct gcatggagcc
     2161 gaccttgaaa taccaccctt taatgttt
//
LOCUS       DAJTXY010000245         2097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTXY010000245 DAJTXY010000000
VERSION     DAJTXY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.250"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            8..703
                     /locus_tag="OO566_004183"
                     /pseudo
     CDS             8..703
                     /locus_tag="OO566_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995299.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(744..1739)
                     /gene="pgl"
                     /locus_tag="OO566_004184"
     CDS             complement(744..1739)
                     /gene="pgl"
                     /locus_tag="OO566_004184"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6607209.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVASYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTATLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          546 a          542 c          489 g          520 t
ORIGIN      
        1 tcgtgatatg aatgcttatc atcgtggtat ggtgacatta gcgtgtattc cgaccgcagt
       61 attttatttt ttaccgctgg caatcggtaa atttaatgag ctgtatccca atattaaagt
      121 gcggattctg gaacaaggca cgaataattg catggaatcg gtgctgtgta acgaatcaga
      181 ctttggcata aacatgaaca acgtcacaaa ttcatccatt gattttaccc cactggtcaa
      241 cgagccattc gtgctggcct gtcggcgtga ccatccattg gccaaaaagt agcttgtaga
      301 atggcaggag ttagtgggtt acaaaatgat tggcgtacgt tcctccagcg gcaaccgatt
      361 gttgatagaa caacagctag ccgacaagcc ctggaagctg gactggtttt acgaagtgcg
      421 tcatctttcg acgtcattag gactagtgga ggcaggactg gggatttcgg cgcttcctgg
      481 tctggcaatg ccccatgcgc cctattcttc gattattggt atcccactgg tggaacccgt
      541 tatacgtcgg acattgggga ttattcgccg caaagatgcc gtgctttctc cggcagctga
      601 acgctttttt gccttgctga ttaatttgtg gactgacgat aaagacaatt tgtggaccaa
      661 tatcgtcgaa cgccagagac atgcgttaca ggagataagt taatacaccg gcgcaggaga
      721 gactgcgccg gttaaatcag cggttagtgt gcgttaacca ccacccacat tggtccctgc
      781 ccgaccgcat agcggccttt ttcatgcagt agcccctgct cgccaacaat ttcgtatacc
      841 gagatgtggt gagatttttg cccagcggca atcagatact tgccgctgta atcaacattg
      901 aagccgcgcg gctgggtttc cgttggctgg aagccttctt tactcaacac gctgccatct
      961 tccgaaacgc tgaaaacggt aatcagagtg gcggtacggt cgcaggcgta taaatggcga
     1021 ccatccgggg tgatatgaat atcagccgcc caacgggtgt cggagaagtt ttccggcatc
     1081 atatccagcg tctggacaca ttcgatattg ccgtgcggat ctttcagttc ccagacatcc
     1141 actgagctgt ttaactcatt gacgcaatac gcatattgtt cgtttggatg gaataccata
     1201 tgacgcgggc cggccccttc aacggtagtc acttccgcag ggtcctgcgc caccagatga
     1261 ccatcatcgc tgaccgtaaa caggcaaatg cgatcttgct ttaatgccgg aacccacagc
     1321 gtacggttgt ccggtgagat attggcggaa tggcaaccgt ccagcccctc gactacatcg
     1381 acgacgccca ctggcaggcc atcttccaga cgcgttacgc tcacgttacc cgcattgtaa
     1441 gaagctacaa agacaaactg cccctggtga tcggtggaaa tatgcgtcgg actacccggc
     1501 agcacagact ctgcggcaaa ggtcagtgcg ccatcgtccg gggcaatacg atacgccagg
     1561 acgcgaaact cagggcgaac accaacatag agataacgtt tgtccgggct gaccaccatc
     1621 ggctgcacct gccccggcac atcgacaacc tgtgtcagcg tcagtgcgcc ttcatgattc
     1681 aggttccaga cgtgaatttg ctggctctca gggctggcga tataaactgt ttgcttcatg
     1741 aacacacctc aaaaatgata aatcccttat ctttcataca tataacaaca cttactggat
     1801 aaaaaacaga caacaaaaca cgataaaata caatatcgca catcaattaa atcaataagt
     1861 tagcactcca tttcgaatgg catattacta aaatattagt caccgacacg aatttgacaa
     1921 tgccacagat attttcgcga ctattcaggg agtatgactc tccccgcttt tgtttacctg
     1981 tatcaacgac cgtaaacact atgccataac aacttctggc caacttccca ttcgctcaac
     2041 aatcaactca cttccggcac aaataaacgc attacgttgt agatcggtat tctggtc
//
LOCUS       DAJTXY010000246         2091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTXY010000246 DAJTXY010000000
VERSION     DAJTXY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.251"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..2058
                     /locus_tag="OO566_004185"
     CDS             <1..2058
                     /locus_tag="OO566_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6607210.1"
                     /translation="PGMPATTKGIRQALQRFSRDLGDVSRRREGTKAIEYHIDCLPEI
                     TRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGSPQLMEERLNALTE
                     NQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPERLQQAAVNANAKR
                     GANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEISWLPEFLAQYRQVN
                     GRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEVVKQKGRVTGSEYR
                     QLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTFVIDGGTRFVVGWS
                     LDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVVGILPRLGIKHPTG
                     IAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRDLRSAFNALQQDKP
                     LNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRHYSPTEFRKKRLAE
                     EDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDEHGRKVLISYDIHD
                     AERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRKRAEEKIRDAEDEG
                     IQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRLGMQKLFAMQEEDD
                     PLA"
BASE COUNT          566 a          501 c          595 g          429 t
ORIGIN      
        1 ccaggaatgc cagccaccac aaaaggcatc cgacaggcgt tacagcgttt ttctcgtgat
       61 cttggtgacg ttagtcgccg tcgggaaggc acaaaagcca ttgagtacca cattgattgt
      121 ctgcctgaaa taacacgaaa ggcactgcgt gaacgctatg tggaacagct ggtggctaca
      181 gagaacaatg tttctgaagt gaaagctgtt accagaaaaa cacgcaatcc tgacgctgtc
      241 caggcaatcg aagcatatcg cggttctcca caactgatgg aagaacgcct gaatgcgctg
      301 accgaaaacc agcgctgggt atctgaagca agagctgcgc tggtggtgga agtgctgaag
      361 ctggaaagcg ccggtaacct cgggcgactg aaagccatta actttcttgt tgaaaaagcc
      421 cgtaaaggtg agctgccgga gcgcctgcaa caggccgcag ttaacgccaa tgcaaaacgt
      481 ggcgctaatc gtactatcag ccgcgatcca ctttaccagt gggttctgaa atacaaccag
      541 tcgcagaacg ccgccgaacg gctgctgtta ctggcaccgg gaaaacgcga cgaaataaaa
      601 ccggaagaaa tcagctggct gcctgaattt ctggcgcagt atcgccaggt gaacggcaga
      661 cccatgtcag aagcctacga ggattttgtc gcagaatggc agcgtcgcca tgcggacgaa
      721 ccgtacatgc tggaggtgat gccttcctat gatgttgtcc ggtatgccat gaaaaaactg
      781 ccggaagtgg tgaaacaaaa agggcgcgtt accggcagtg aataccgtca gcttgaagga
      841 ttcacccgcc gcgactggac agctatgccg gtgaattatg tctggattgg tgacggtcac
      901 ggtatgaagc tgaaatgtgc gcatccgatc cacgggcgtc cattctcacc ggaagtcacg
      961 tttgtgatcg atggtggaac acggtttgtt gttggctgga gtcttgacct tgccgaaaac
     1021 gtgttcgccg ttgccggtgc gatacagcac ggcattcgca accacggaaa accgttcctg
     1081 tattactcgg ataacggttc cggtgaaacg gctgacatgc tggacaaaga ggtcgtgggt
     1141 attctgccac gcctggggat taaacaccct accggtattg ccggtaatcc acagggacgc
     1201 ggcattattg aacgcctgaa ccggacactg ccgatgcgta tagccaggag gtatcgcacc
     1261 tattttggca agggtgctga ccgggaatcg ttgcgtgtac ttaaccgtga tctgcgttca
     1321 gcgtttaacg ccctgcaaca ggacaagcct ctgaacgacc gccagaaagc ggcaatgcgc
     1381 gagctgccat cgtgggcgga gcttattgag gctattcgtg aaggcgtgga atggtacaac
     1441 aaccgcccgc attctgagct gccgatgaaa ccgaacggac ggcattacag cccgacagag
     1501 ttcagaaaaa aacgcctggc agaagaagac accgaaattg agtggctgag tgatcttgag
     1561 ctgcgcgaca tgttccgccc aatggtggaa cgaccggtaa gacgctgcga aattcagtgg
     1621 ctgaacaata tttactacgc gccggagttg cgtgacgaac acggtcgcaa agtgcttatc
     1681 agttatgaca ttcatgacgc cgagcgaatt acggtacgtc gcaaggacgg cagctttatc
     1741 tgtgaggcga tatggaacgg caataaacgc gcagcctttg ctgtcagtgc tgaatatcac
     1801 aaacaacagc agcgtattaa aggaatgcgc aaacgcgctg aggaaaaaat tcgtgatgct
     1861 gaagatgaag gtattcagat tctggaacac aaacaggctg aaccctggct gagtaatgtt
     1921 tatcgcccgg ttggtaatgt ggtggcagtt cagcaaccgg aatatgaaga agagcatgac
     1981 gaagaatttg aacgcgattt ccggctgggg atgcaaaaac tttttgccat gcaggaagaa
     2041 gatgatccgc tggcctgaat aaaaaaacag tccgcgggaa cggactgtta a
//
LOCUS       DAJTXY010000247         2089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTXY010000247 DAJTXY010000000
VERSION     DAJTXY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.252"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(60..1025)
                     /locus_tag="OO566_004186"
     CDS             complement(60..1025)
                     /locus_tag="OO566_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR6607211.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="OO566_004187"
     CDS             complement(1879..>2089)
                     /locus_tag="OO566_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607212.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVRIDLNGIKFGFLRTHS"
BASE COUNT          577 a          551 c          436 g          525 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcataac gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatacccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggga taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattctc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aattaccgct tcacttctt
//
LOCUS       DAJTXY010000248         2086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTXY010000248 DAJTXY010000000
VERSION     DAJTXY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.253"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..710
                     /locus_tag="OO566_004188"
     CDS             <1..710
                     /locus_tag="OO566_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6607213.1"
                     /translation="VTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPV
                     ALTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAEL
                     DADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHR
                     ALAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRA
                     SVIRTTRKIFSGEVYLP"
     gene            786..2086
                     /locus_tag="OO566_004189"
                     /pseudo
     CDS             786..2086
                     /locus_tag="OO566_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
BASE COUNT          489 a          474 c          535 g          588 t
ORIGIN      
        1 cagttacccg tgtacgtatc cgcaacgtca atacgggtac gttcatcgaa gctgatgtgc
       61 aaacgccaaa tggtgttgtc gagtacgagg gtagcgccag aattgacggc gtaccgggta
      121 ctgccgcacc ggttgcgctc actttcctga atgccgctgg aacgaaaacc ggaaaagttt
      181 tcccgactga taatcagatt gattattttg acgatgtccc ggtgacctgt atcgatatgg
      241 cgatgccagt cgtcattatt ccggctgaat atctgggaaa aacaggttat gaattaccgg
      301 cggaactgga tgccgacaaa gcattattag cccgcattga atctatccgg ctacaagcgg
      361 gtaaagcaat gggcttaggt gatgtcagta atatggttat ccctaaacct gtgcttattt
      421 ctccagcgca gaaaggcggg gcaattaatg tgcgttattt tatgccgcat tcttgccatc
      481 gcgcgctggc gataaccggt gctattgcta tttccagtag ttgtgcattg gaaggcaccg
      541 tcacccgaca aatcgtccct tctgtaggat atggcaatat caatattgaa caccccagtg
      601 gtgcgctcga cgttcattta agtaatgaag gtcaggatgc cacgacgtta cgcgcatctg
      661 ttattcggac aaccagaaaa atattttccg gtgaagttta tcttccctga aaaaattcgt
      721 tgtcaggata aggacaatca ataaaggact tctgtatgag tcatacagaa agaacaggat
      781 tttaaatgaa taagaaatcg ttatggaagc taattctgat attagcgatc ccatgtatta
      841 ttggttttat gccagctccg gcaggattaa gcgaactggc gtgggtgctt tttggtattt
      901 acctggcggc cattgtgggt ctggttatca agcctttccc ggaacctgtc gtactgttaa
      961 ttgccgttgc tgcctcaatg gtggtggtcg gtaacttatc cgacggtgcg tttaagacca
     1021 ccgccgtatt aagcggttac tcttcaggta ctacctggct ggtgttctcg gcgtttacct
     1081 taagcgccgc atttgtgacc accggtttag gtaaacgtat tgcctatctg ctgattggta
     1141 aaatcggtaa caccatgctg ggtctgggtt acgttacggt attcctcgat ctggtactgg
     1201 ctccggcaac accgtctaac accgcgcgtg cgggcggcat tgtgttaccg atcatcaaca
     1261 gcgtggcggt ggctttgggg tccgaaccgg aaaaaagtcc gcgtcgtgtc ggacattacc
     1321 tgatgatgtc catttacatg gtcaccaaaa ccaccagcta tatgttcttt accgcaatgg
     1381 cggggaacat tctggcgctg aaaatgatca acgacattct gcacctgcaa attagctggg
     1441 gtggatgggc gctggccgcc ggattgccgg attgccggat tgccggattg ccggattgcc
     1501 gggcatcatt atgctgctgg tcaccccgct ggtaatttac accatgtatc caccagaaat
     1561 taaaaaggtg gataacaaaa ccatctctaa agcgggcctt gccgaactgg gaccgatgaa
     1621 aatccgcgaa aaaatgctgc tcggtgtctt tgtgctggcg ctactgggct ggattttcag
     1681 taagtctctg ggggttgatg aatccactgt ggcaatcgtt gttatggcga ccatgctgct
     1741 gctgggtatc gttacctggg aagacgtggt taaaaataaa ggcggctgga ataccttaat
     1801 ctggtacggc ggtattatcg gcttaagctc cttattatcg aaagttaaat ttttcgaatg
     1861 gttagctgaa gtctttaaaa ataacctggc atttgatggt cacggtaacg ttgctttctt
     1921 cgttattatt ttcctcagca tcatcgtgcg ttatttcttc gcttccggta gtgcctatat
     1981 cgttgctatg ttacctgtat ttgccatgct ggcgaacgtc tccggcgcac cgttaatgtt
     2041 aaccgcgctg gcactgttgt tctccaactc ctatggcggc atggtt
//
LOCUS       DAJTXY010000249         2083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTXY010000249 DAJTXY010000000
VERSION     DAJTXY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.254"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..509)
                     /gene="yidZ"
                     /locus_tag="OO566_004190"
     CDS             complement(<1..509)
                     /gene="yidZ"
                     /locus_tag="OO566_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6607214.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLA"
     gene            complement(484..1659)
                     /gene="mdtL"
                     /locus_tag="OO566_004191"
     CDS             complement(484..1659)
                     /gene="mdtL"
                     /locus_tag="OO566_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6607215.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1791..>2083)
                     /locus_tag="OO566_004192"
     CDS             complement(1791..>2083)
                     /locus_tag="OO566_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6607216.1"
                     /translation="HGGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVII
                     PAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          584 a          557 c          545 g          397 t
ORIGIN      
        1 atggctaacg gtagcgagct taacagctcc cgcgagcgag gatggctttc gcgaccggaa
       61 aaaccaatat ccacttcacc acgagtaatg gcatctaagg aatcgtaatc ccagttacgt
      121 agtttgatgg tcgcctgcgg gtaacgttgg tagatccgtt tcgacagcgc attaagcatg
      181 atcatcatca gcggcgattc cgccgccaac tcaaacttca ggccgcgcgg tgtctggtgg
      241 tgcggtttat ccagcagcag attgctcatt tgcatccatt ccgccagatt ttgctccatg
      301 ctgaccatta gctgtgtggg cgacagcccc agcggtgagt tcacaaagag cgggtcgtca
      361 aaccacgctc ttaactttgc cagcgactta ctcaccgctg aaggggtcac gttcatccgc
      421 ttcgccgctt tggtcacgct gcgctcctgc atcagcagtt gcagacagag cagcagattg
      481 agatcaagcg tggtgatgga tttcttcatg agcggcaacg gggcgtccag gcgcgacgaa
      541 cataatcagc aacaggctca ctatgctaca ggcaatcaga atcccgatca gcatattcca
      601 tgcgccgata ccaaccaccg cggccagcca aatccacagt gacgaaccgc aaacctgcgc
      661 aatacctaag gtcgagctgg ctacgcccgc gcgtaatgaa aatggtccta acgcctgact
      721 catcgctacg ccaaaaccta ccgagaaacc ggcgcaaatc agcgtgatac caaacagaga
      781 aaccgcatgg gaaggtgaaa cggcaagggt gatccccgcc gcaaggaata acacctgcga
      841 ggtgatcatc aacgtacgtg gcttaaaaat tcccagcgca aatggcgtcg agaatgaaaa
      901 ggtcatgctg acgccagcgg tcagcgccat aatggtggcg tattctccgc gctcaaaccc
      961 catgatttcc atcagcaata ccggcgatgt attgacgaaa gtgaggatca ccgaaacgct
     1021 gagggtggtg ataacaacac ggctgaggaa aaaacgatta agcagcgact cgctattttc
     1081 tcgtggtttg tccgaagcag cgggggacgc tgggcgcgtt tcttttaaaa taaacaaaga
     1141 caacatcagt accgcgatgc ccatcattgc catcgcccag aacagactct gccacgggaa
     1201 tttaagcata atcagatgtc cgagcactgg cgctaacacc ggaatgatgc aggtaatacc
     1261 gttgagtaat gacagcactt tagcccgacg tcgatcatcc agcgtgtcgc gcaaaatggc
     1321 gaacgccacc acgtaacaac agcctgcgcc caacccctgt agaaatcggc ctgcaagaaa
     1381 taacgtgctg gtttcagcca gtgaacagaa caccgaggca ataataaata gcgccgcgcc
     1441 gggtatggcg accggctttc tccctgaacg atcggccact ttaccggcaa atagcatcgc
     1501 agctgccatc cccgccagat atacggagaa cgcaatatgc aactgcgctt cgctggcatt
     1561 gagatcggcg gcgatgcgcg gtaaaccaac gaggtacata tcaatcccgg cgggataaag
     1621 taaaaccagg gcaaaactac aaatcaaaaa gcgggacatg ggctaaagcg tcctgatagt
     1681 aaggtgacgc aaagcatacg tggtgaagga ggataaggcg agttgccatt tggacataag
     1741 tgatttcctg agaggcaaga agccagcgaa tggctggctt cttgaaggat ttagccaaat
     1801 ttaggtaaca cgttaaagac gttgccgaac cagcacaaaa tcacggttat accaaagaga
     1861 atgacaatcg ccggaataag attgccgccc cagaccgtga acatctgatt gggaaacttc
     1921 ttgcgagctt tgattgcaag cactgcggga ataatgaccg cccagatggt ggcgcacagc
     1981 ccggcaccgc cgatcccgta aataaagccg ttcgggaaga tcagatacaa caacgcaggt
     2041 ggcaggaagg ttaacagcac ggttttgaaa cgcccgccgt ggg
//
LOCUS       DAJTXY010000250         2045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTXY010000250 DAJTXY010000000
VERSION     DAJTXY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.255"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(163..360)
                     /locus_tag="OO566_004193"
                     /pseudo
     CDS             complement(163..360)
                     /locus_tag="OO566_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(492..2015)
                     /locus_tag="OO566_004194"
     CDS             complement(492..2015)
                     /locus_tag="OO566_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6607217.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          609 a          455 c          438 g          543 t
ORIGIN      
        1 tatttcggtg atatataaaa acaactgtga taacaccaag gttgacaact atttgcgcaa
       61 taattccttt ccttcaatga tccaggcgtt cactgccagc aacccattca gccgcatact
      121 gacagtgaga aactgaaaga ttattgctga tccctgtggc tgacgagtct ttctcttaaa
      181 tcgattaacc agattgatcg ccagccaaac aagggcatta agcaaattta tcgtcacaag
      241 cacttctccc agcccggtac tgggttttgc aataaggatg accaggaggg tgatcagcat
      301 aaatgcaatg gaaaagatga tatagagagt gaaataatcc agattattac tattgttcat
      361 gctactaatg gcaatcctgt caaaactttc aaacaattat agcaaacaaa ataatgaaca
      421 atagtaacaa gaggcaacga gctaattagc tgtctataat tacaccgcac tatggcctta
      481 ctgatcaatt ttcactcaac cacaaccttc accttcggtt tagaaaggat aattttgacc
      541 aggtcgatgt atgtaacagg tttatagaaa taataaccct gctgaaatgt gatattattt
      601 tggttcagat agccaagttg ttctttcgtt tcgacacctt cagcgacgat actgatcgaa
      661 agtttacgcg ccagatccaa tacacaatcc agaattcggg ttgattctgg gtctgcgttt
      721 acgcggccaa cgaaactgtg atcaattttg atataatcaa tatgcaggtc atgaagataa
      781 gaaagccctg agtagccagt accgaaatca tccagcgcga tgacaaaacc attttcatgc
      841 agaatgttca accgctgaac cagactttca tcaacattca atggctcacg ctcggtgact
      901 tccagaacaa ggtttaaatc gcggcgggta aaactgtcac ggaaatttaa acactcgtcg
      961 acaaacgtcg gcgaaataat atgcgaagcg ctaaaattaa ttccaatatg aaaaccttcc
     1021 ggcagtttac tcgcgatagc gttcatctgt ctggcaacct gattcatcag gctttgtgta
     1081 agcggaacga ttaatccgga tttttcagca agtggaataa atgccgcggg tgaaatatat
     1141 ccaccgtgag gttgtttcca gcgggctaac acctcaactc cccgcaatgt cccttcccga
     1201 ccatttacca caggttggta aaaagggacg atctccccat tatttattgc ccgtcgcagg
     1261 atctcttcag gcgttgcgct tttattgaaa tatctatcaa gcaggaaggc agcggcgcag
     1321 gcgattaaca gaataaatat taaaatccca aagcctttat cgataagtct gtagaagcta
     1381 aagagtggcg gcgggttaaa gataatagtg aaatgatatt ttgtggagtg aaccttctcg
     1441 gtatagggat aatgtccatt aagcgtcatc acatcacccg ttggtccaat aatgtcatcc
     1501 gccacgcgta atacatacct tacccctttc aagggtacat taagcgcccc gcgaatatgt
     1561 tgatcactta tcgtaaccaa aatgcgcgta tctgcaaatt ggctctgata gagcaggata
     1621 ggtaatctat taacggtgtc gattgccgga gccaacagta aattactgtc cgggacaaca
     1681 ggaatacgag acagcaggac ccgattccca ggcagggatg tacaccagac tattccctgt
     1741 ttgagaatga tgattgtgcg caggtgaggt ttcagagctg cttcagtgcc aagctgatat
     1801 tgctcctcga ggtcgcattc cttcccggca atgttaaccg ctgtttgagt cgcctgtgac
     1861 gcttcttcaa gtatatgatt gatattgttg gcggcatatc ttgctcccgc caggtactct
     1921 gcttttgcgg aataccagag ctggatgttc aaaatagcga caccggtaat aaacaagcat
     1981 atcgcaacga ggatgggtat gagtgtattg cgcatttgct tgtcccggcc aaagggttta
     2041 ataca
//
LOCUS       DAJTXY010000251         2032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTXY010000251 DAJTXY010000000
VERSION     DAJTXY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.256"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(210..374)
                     /locus_tag="OO566_004195"
     CDS             complement(210..374)
                     /locus_tag="OO566_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607218.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1276..1560
                     /locus_tag="OO566_004196"
     CDS             1276..1560
                     /locus_tag="OO566_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6607219.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1548..>2032
                     /locus_tag="OO566_004197"
     CDS             1548..>2032
                     /locus_tag="OO566_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6607220.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          522 a          426 c          583 g          501 t
ORIGIN      
        1 ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga gccggttact
       61 ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt ctgcaatacc
      121 ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata gctgatggag
      181 ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc gtttccggcg
      241 taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt atgagagatg
      301 aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt aagagcggtg
      361 gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt gcggagatga
      421 acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc gaaaatgtgc
      481 tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc ggcatccggc
      541 atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc tgtcgtctcg
      601 atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg atgcgagaca
      661 caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg agcgaagggg
      721 gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc catgagtcag
      781 ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg ggtcaaagct
      841 aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt taccaatcga
      901 agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt gcccgcaaac
      961 acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga attcgtcacg
     1021 actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg gttcaacgac
     1081 aagagccgta tgacgcgaga gtgtcacgta cggttccgtg ggagcgtagg ggggcggtgc
     1141 ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta aatgccgctg
     1201 acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg tcaatatgct
     1261 attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca gctcaatctc
     1321 agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct tcataagtcg
     1381 cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat tctcgaccgg
     1441 cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct tgatgctccg
     1501 gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg ggatgtgtaa
     1561 ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc ggtgaagctg
     1621 tacttgatga ctggttaaag cagaaggggc taaaaaatca ggcaatcgga gccacccgga
     1681 cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca ctggccactg
     1741 gtagtatcaa tcatacagaa gctacaggta accttcggcg caatatgcca gaccccattc
     1801 ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa gggcttggcg
     1861 cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat atcggtgtgc
     1921 gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt catcatggtt
     1981 ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa ta
//
LOCUS       DAJTXY010000252         2017 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTXY010000252 DAJTXY010000000
VERSION     DAJTXY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.257"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            362..666
                     /locus_tag="OO566_004198"
                     /pseudo
     CDS             362..666
                     /locus_tag="OO566_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            835..>2017
                     /locus_tag="OO566_004199"
     CDS             835..>2017
                     /locus_tag="OO566_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6607221.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          533 a          402 c          456 g          626 t
ORIGIN      
        1 cgtataaaca tctttgacgc cagcgagata cagccatcct tcccgggtgg caacatacgt
       61 tatatctgcg acccagacct gattaggggg gttggggagc aatggaacag taaacgccgt
      121 taagaagtca atttaaatag ccaaaaatta actagccaat ttacattgca ggttgtaatt
      181 tttaaccacc ttttttacga tagccaaatt acctataccc atcccggccc tgccaagcca
      241 tacccgctat cggcgatgaa aatgcttttg tgcgcggtcg gagtggtccc gacgagggtt
      301 ttacccgaag tcggggcgta tctccgcgtt agcgggccgt gagggccgct tacgagcgtg
      361 tatgagagac gtacagtggt tttgtccgct cacaccagta catacaggta cggaaataaa
      421 accagtcgaa atgccggtaa cgacgattac tcctgtctct gatgccggtg gactacaaga
      481 ttttatttac tggcgtcctg atgcggacag gactggtatg gaacctgttt atgtgatact
      541 taatgatcct ctggattccg ggcgatttac gcgtaaacaa ctaaataaaa atatcttaaa
      601 catgcaagta attttggtat tctcgataca agaataaata gtagtaacta ctatagttgc
      661 aaataatgtt aaaaacattt ctatgatcat ctttacattt tttatgattg acaattgttt
      721 ttggttaaaa aaagacaact aatgagtgtg ataatgcaat actttgagta attatataat
      781 gataagtaaa caggtttgcg acaattggtt atcagtatta cagaaggatt aaatatgtca
      841 ttattcacag aacctaagga aattgaaaga ttaccctctg aagagattga acgattatat
      901 cccgtcctgc gatacagggt ctttatcagc attttcctcg gatatatggg gtattatttt
      961 gtccgaaaca ccaccagtgt gttatccggt gtgctgcaca tgtctgcaac cgagatcggc
     1021 attatctcct gtgccggttt tttatcctat ggtatatcaa agtttgtttc cggacttatt
     1081 tctgacagaa gtaattcaaa agtatttttg tctcttggtt tattcctgtc cggactggta
     1141 aattttctga taggatatat tcccgggatc atcacatccg ttacgttatt ttcaacaatg
     1201 tatttactta acggctggat ccaaggtatg ggatatcctc ctggtgccaa aacactggtg
     1261 ttctggtatg aacatcggga aaggatcact tgggccacac tgtggaattt atcgcacaat
     1321 gtcgggggag ctctggcccc cgttctgatt ggtttcagtt ttggtttttt cggcgacagt
     1381 gctctggagc atgcccgggc cgcatttatc ttcccaggag tactgtgtat ggcaatgagt
     1441 gtgctgattt actttattca ggtggacaga cctgtatcag tggggcttcc tcccattgaa
     1501 gaatggaaag gtaacgtcgt ttcacacccg gctaaaggcc gtgaacaagg tccgagactc
     1561 tccattcctg atatcatcag aaaacatatc atccgaaata ataaactcat ttactgctgt
     1621 atctatggtt cgtttgttta tattctgaga tacggtattg tttcctgggc tcctaagttt
     1681 ttaagtgaca gtctggatgt cggtggtaag gatatgggaa aactggcatc aatggggggg
     1741 ttttctgttt ttgaaatcgg tggtgtggct ggtatgcttc ttgccggata cctgtctgtc
     1801 agactgttca gaaactcaaa accattaaca aatactttat ttctggcact taccattatt
     1861 ttattaatcg cgtactggta cgtgccatca ggcaatgaat atctctggct aaactataca
     1921 atccttattc tgttggggct ggcagtgtat ggtccggtga tgtttattgg tctttattca
     1981 atggagcttg ttcctaaaga agctgccgga gccgcat
//
LOCUS       DAJTXY010000253         2011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTXY010000253 DAJTXY010000000
VERSION     DAJTXY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.258"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO566_004200"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO566_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6607222.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO566_004201"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO566_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="OO566_004202"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="OO566_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          593 a          372 c          453 g          593 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaacacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt gagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttat gttgtaattt
      901 atttatggag gttttctatg gaattctttt ttattgtgac tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgata aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt aaggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt accgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagta ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc a
//
LOCUS       DAJTXY010000254         2009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTXY010000254 DAJTXY010000000
VERSION     DAJTXY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.259"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..459)
                     /locus_tag="OO566_004203"
     CDS             complement(<1..459)
                     /locus_tag="OO566_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6607223.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCS"
     gene            complement(1060..1308)
                     /locus_tag="OO566_004204"
     CDS             complement(1060..1308)
                     /locus_tag="OO566_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6607224.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1663..>2009)
                     /locus_tag="OO566_004205"
     CDS             complement(1663..>2009)
                     /locus_tag="OO566_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6607225.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          507 a          378 c          484 g          640 t
ORIGIN      
        1 agaacataat tgattatttg agacatgaag ctccttcaaa gatatgggga gtgtgggtag
       61 tgtgattagt tgattgtggg acgcattcaa taatttaagt ccttgaggca aagcaggcag
      121 ttcaataagt ctgttatagc tgacattaag ttgtgtaagg gacgcaggca atggggagat
      181 taagcttaaa ttatttttac ttatattaat tgatttaatt cccgggggga tttcaggtaa
      241 tgttgtcagg cctaattcag acaagtccag gttcgtctct tggttttgta gacatgatac
      301 tagtcgctga aaagcgatgt ctcgttgttc ttcttgtatg cggttgtttt tccattcagt
      361 ccaatgggtt agataatttt catatgcgcg gctagtgtcg attgaataag tggagaacga
      421 atttgaaatt aatctgtgat tgttatttgt cgggagcata aatatcaggg cgtattcttt
      481 atgttgagag gtgttggatt cttttttatc tgctttgcaa tgttattgat tcccttcagc
      541 agtagacagg aacttctgga ggaggcggat atatagtttg tgtaagcata ttaactccat
      601 atgttatata ttgaagaact tctgctttac actatcctaa actggatcgg tttaatgtaa
      661 aaaaacgata ctttttgggg gagggggcaa tttttcctta aggtatgaaa aaaggagctg
      721 aagctatata atagctatta atgccactgg tatcagtgaa tcatgtatat aaaaaactgt
      781 gttaaggagg ctgtccatta gcggtttttt attcttctgg aaccgcggca tctttgcgta
      841 tctttttgtc ttctcaccgt ctggtcggtg ttttgctgag actgctaatt tcctgtcttt
      901 gctagtgctg tactggtact gcccaatcat gattggcggg ggcgggcgga gttgagaccg
      961 cagacacgtc gtatgcaaga acgtgctgcg gctggctggt gaacttccga tagtgcgagt
     1021 attgaatgat ttccagccgt tactgatttt acgtgctaat tagtgaacaa accactcgtc
     1081 agcagactcc caggtatctt tcagagtttc ctgaacaaaa gttttagctg aatctttatc
     1141 ggcggtgcgc gtaacagaaa ggccatcgtt gctggtggct tttacgatca cctctacatc
     1201 gtcataacgt ttactgatgc gtcgggttaa ttcttccttt aacgcatcca cagcaccggt
     1261 tggcatttta gtcattttct ctttggctat gcagatttca atacgcataa aaatccctct
     1321 acactgtgtt tttatacagt attattttta actgtatgga taaacagtgt caagagtgag
     1381 ggaaagtgat tttttggggc atgcatgggg caaaaatatt tgttgtgggg cattttgggg
     1441 catgattggg acactttgtt acatatgaac tttgccgatt ttcatatgac atggcaatac
     1501 aacgctttga ttattctaaa gaaaacacat gctcttgggc gttctttagt tatttttaaa
     1561 aatcccgtat cacgcagtta aagtggcggg catactcttc aaggctggtg atccccaacc
     1621 gcacccattt aggatgcgac cattgcggta gaccgatgta aatcataacg cgtgaaggat
     1681 ctcttccacg ctacgcacac gggcgatgcg cgggtagata tgattaatgc tgttattgtg
     1741 ttgctcggcg ctggcggcac tacaggcgtc ttcggcaatc accagattaa agccaagttc
     1801 ccaggcattg cgggcggtgg attcaacacc gatattggtc gagatcccac ataacactat
     1861 tgtatcgata cctcggcggc gcagttgcaa ctccagatcc gtaccgtaaa acgcacccca
     1921 ctgacgtttg atgatttcga tatcactgtc agttgcacct aatgcagctg gatgttgcca
     1981 ccagttttcg ggcaacactt tagcggggg
//
LOCUS       DAJTXY010000255         1991 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTXY010000255 DAJTXY010000000
VERSION     DAJTXY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.260"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..191)
                     /gene="ospE2"
                     /locus_tag="OO566_004206"
     CDS             complement(<1..191)
                     /gene="ospE2"
                     /locus_tag="OO566_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HCR6607226.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTAT"
     gene            complement(373..774)
                     /locus_tag="OO566_004207"
                     /pseudo
     CDS             complement(373..774)
                     /locus_tag="OO566_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1203..1991
                     /gene="virF"
                     /locus_tag="OO566_004208"
     CDS             1203..1991
                     /gene="virF"
                     /locus_tag="OO566_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6607227.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          618 a          351 c          375 g          647 t
ORIGIN      
        1 gtagcagtga atttcaacgt tttcgtccgg ttggcagcag gtatttgtaa ttcgtatata
       61 gctgcttttt tattgaaaac agtatatcca tctgtagtaa cagtagaaga taataataca
      121 ggatttgata cttcaaggat gatattttct tgtttttgtg gaaggcaagg gaatatagtt
      181 tgtgtaagca tattgcttcc ttatgttata ttttagtaaa gaatttattt tttacattat
      241 agtcggctaa atgaagttta ctgtaaaaaa acgatacttt tttcgtaagc gatgagatgt
      301 ggagccagaa aaatggctgc gttacgtcat tgaactgtcc ttgacccacg atattcagac
      361 agtcgttgta tcttaagttg acattacccg ctgacgtcga tattcccttg gtgagagata
      421 acccaacgca ctatgcggat ggttttcatt gtaatgctcg atcgccactg caaggttatg
      481 caatgccgtt cttggtttcg gcatgaacgc gatgcagtct tccttcatcg ttttcacgaa
      541 ccgttctgct attccattac tctgagggct actgactgcc gtcgtgcagg gctccagatt
      601 caactctctg gcgaactgcc gtgtttcatg agccgtataa gccgaaccgt tatcggccac
      661 tggattggcc gttccggtcc tttatcgcca tatcactcct gacatgacat cctgcaccgt
      721 tgctttgtca taatctcccg tgcctgctgc ccagtctatg gcttcacgat cgcaatatgg
      781 aacgtaacgc tgtcatcggg agaatcgtca atgcggcatt cggttaacgc ttaccaatat
      841 caacaccaac cggatttgtg gagcctccag tctgaagggg ctttatgcgt tccgtatagg
      901 atattatgat gctggagttt tgcgaagctc cactcctttc cgaacattta cacagatatt
      961 gcctaagaaa agtgaaacca taatatggtt atagtccctt tcagtgcaaa tacttagctt
     1021 gttgcacaga gaaatagaag ctgcataagc tctttcttca aaaaatgtaa ataaagttaa
     1081 atataggaaa aattacttaa tctatcttaa taacggaaag attttgtata caatcactgt
     1141 tacagaaatt tcttagttac tctgtaaaca ctaaatatag tttggtatat tctgttgaat
     1201 ttatgatgga tatgggacat aaaaacaaaa tagatataaa ggttcgcttg cataactata
     1261 ttattttata tgcaaaaagg tgttcaatga cggttagctc aggcaatgaa actttgacta
     1321 tcgatgaagg gcaaattgct tttatagagc gaaatataca aataaacgtc tccataaaaa
     1381 aatctgatag cattaatcca tttgagatta taagccttga cagaaattta ttattaagca
     1441 ttattagaat aatggaacca atttattcat ttcaacactc ctattctgag gagaaaaggg
     1501 ggttaaacaa aaaaatattc ctcctctctg aggaggaggt ttctatcgat ttgttcaaat
     1561 ctataaaaga gatgcctttc ggcaaaagaa agatctatag tttagcttgc cttttatcag
     1621 ctgtttctga tgaggaagct ttatatactt cgatatcgat agcttcttct cttagttttt
     1681 ctgatcagat aaggaagatt gttgaaaaaa acatcgagaa gagatggcgt ctttctgata
     1741 tttcaaataa cttgaattta tcagaaatag ctgttagaaa acgattggag agtgaaaaat
     1801 taacatttca acaaatcctt cttgatattc gcatgcatca tgcagcaaag cttttattga
     1861 atagtcaaag ctatattaat gatgtatcaa gacttatcgg aatatcaagc ccatcttatt
     1921 ttataaggaa atttaatgaa tattatggta taactccaaa gaaattttac ttatatcata
     1981 aaaaatttta a
//
LOCUS       DAJTXY010000256         1969 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTXY010000256 DAJTXY010000000
VERSION     DAJTXY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.261"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..1017
                     /gene="curA"
                     /locus_tag="OO566_004209"
     CDS             <1..1017
                     /gene="curA"
                     /locus_tag="OO566_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6607228.1"
                     /translation="NRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLRTVYLSLD
                     PYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWGLGYSGWQDYDISS
                     GDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATGPVGATVG
                     QIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYLADDFAEQLAKACPKGIDIYYEN
                     VGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKRIRLQGFI
                     IAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNFGKVVIRV
                     AGDD"
     gene            1215..1880
                     /gene="mcbR"
                     /locus_tag="OO566_004210"
     CDS             1215..1880
                     /gene="mcbR"
                     /locus_tag="OO566_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6607229.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          535 a          421 c          528 g          485 t
ORIGIN      
        1 aatcgacgtt gggttctggc ctcgcgtcca catggcgcac cggttccgga gaatttccgt
       61 cttgaagaag atgatgtcgc cacaccgggt gaaggacagg tgttactgcg cacagtttat
      121 ttgtccctgg acccgtatat gcgtggacgt atgagcgatg agccatctta ttcaccgccg
      181 gttgatattg gcggcgtgat ggtcggcggt acggtgagcc gtgtcgtgga gtcgaatcat
      241 cctgattatc agcctggcga ctgggggctg ggctacagtg gatggcagga ctatgacata
      301 tccagtggtg atgacctggt gaaacttggc gatcatccgc aaaatccatc gtggtcgctg
      361 ggtgtgctgg ggatgccagg ctttaccgct tatatggggc tgctggatat cggtcagcct
      421 aaagagggcg aaacgttggt ggtagctgcg gcgacaggac cagtgggggc gacggtgggg
      481 caaatcggca aacttaaagg ttgcagagtg atgggggtag ccggtggcgc ggaaaaatgc
      541 cgccatgcta ccgaggtgtt aggcttcgat gtttgccttg attacctcgc ggacgatttt
      601 gccgaacaac tggcgaaagc gtgcccaaaa ggtattgata tctattatga aaacgtgggc
      661 ggtaaggtat ttgatgcagt gctaccatta cttaatacat ctgcgcgcat tcccgtttgt
      721 ggcttagtga gcagctataa cgctacagag ctaccacccg gtccggatcg tttacccctg
      781 ttgatggcaa cggtgctgaa aaaacgtatt cgcttgcaag ggtttattat cgctcaggat
      841 tatggtcacc gcatccatga gtttcagaag gagatggggc aatgggtgaa agaggataaa
      901 atccactacc gcgaagatat tactgatggt ttagagaatg cgccacagac gtttatcggc
      961 ctgctgaagg gtaaaaactt cggcaaagtg gtgatccgcg tcgcgggtga tgattaaagt
     1021 ttcaacggcg gcgtaagccg ccataaaaat ccaggcaaaa agcttatttt cagctttaat
     1081 taactaacca cgtcattgtt ctgctgctaa taacaacaaa ggtgggtggt ttatgattat
     1141 tcactttaat acactaggtg aactccttct gccatgcagg cagggttgga cagaaaacac
     1201 gcgaaggaat taaaatgcct ggaacgggaa aaatgaaaca tgtcagtttg actctgcagg
     1261 ttgagaacga cctgaaacat cagcttagta ttggcgcatt aaaacctggc gcacgcctga
     1321 ttactaaaaa tctggcggag caattaggta tgagtattac acctgtgcgt gaagcattat
     1381 tacgtctggt ttcggtgaat gcgctttctg tcgcacctgc acaagcattt acagtaccgg
     1441 aagtgaggca aagtcagtta gacgaaatca atcggatccg ctacgaactg gaattaatgg
     1501 cagttgccct ggctgttgaa aacctctcct cgcaagacat agcagaactt caggaattgc
     1561 tcgggaaatt acagcaggcg caagaaaagg gcgacatgga acaaatcatt aatgcgaaca
     1621 ggctatttcg cttagcgatt tatcatcgct caaatatgcc catcctgtgt gagatgattg
     1681 agcaactgtg gatcaggatg ggacctgctt tacattatct ttatgaagcg attaacccag
     1741 cggaattacg ggagcgcata gaaaactatc atctattact cgctgcgtta aaagcaaaag
     1801 acaaagaggg atgcaaacat tgtcttgctg aaattatgca acaaaatatt gctattttat
     1861 accagcaata caatcgttaa attaaatgcc cggcaaatac cgggcatcaa gtaattactc
     1921 aaatcgccac gcaatattca tgccgacacc ataatttcgt ccgggcgca
//
LOCUS       DAJTXY010000257         1930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTXY010000257 DAJTXY010000000
VERSION     DAJTXY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.262"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            70..630
                     /locus_tag="OO566_004211"
                     /pseudo
     CDS             70..630
                     /locus_tag="OO566_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            745..1086
                     /locus_tag="OO566_004212"
                     /pseudo
     CDS             745..1086
                     /locus_tag="OO566_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1069..1906
                     /locus_tag="OO566_004213"
                     /pseudo
     CDS             1069..1906
                     /locus_tag="OO566_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          480 a          467 c          585 g          398 t
ORIGIN      
        1 taaaatatta tcagatgact gcttagaaaa agaacaaatg gtggtgtccg ctattgccag
       61 tacacctcag ttctgggcct tgcccgtagc agttattttt atcaccgggc tgcactgaaa
      121 gccggtgata aatacgccac gatacgtaag atgctgacag atatcacgga gttccagctc
      181 cctgcaggta aagtctggct gtcaccagtg gtggactgct tcgatggaaa agttgtgagc
      241 tggtctctca gtacacgccc tgatgctgaa ctggtcaaca ctatgctgga tagcgctgtc
      301 gaaacgttaa atgctggcga acgaccggtg atacacagtg acagaggtgg gcattatcgc
      361 tggccaggct ggctggaaag agtgaatgca gcaggtctta ttcgctcaat gtcccgtaaa
      421 ggatgttcac ctgataatgc tgcatgcgaa ggctttttcg gcagactgaa aacggaaatg
      481 tattatgggc gtaaatggtc gggcatcacg ccagaaaagt tcatgcagca agtggatgct
      541 tacatcagat ggtataacga gcggcgtata aaattatcgc tgggtgccgt cagccccaaa
      601 atgtaccgcc aacaatgcgg gctggaatga taaagcagtc caggaaatcg tccgcatccc
      661 cccttcacca tttggattgt ccgctccacc caacatgttg tttccttaag gttcctacac
      721 cacaaaggaa actctcatgc tgcgcagtgg atggctgacg ttgccgacag gcgggaactt
      781 cgccagttca ggcagacacc ggaacagcgc ttcacgcagg aacaggagca tctgcagccg
      841 ctgctgggta cggacttcga tatccgtcat gtctcctggg atggctatat cgaggttggc
      901 gggaaccgtt acagtgtgcc ggaaagcctg tgtggtcagc tggtatcaat acggatctcg
      961 ctggatgatg aactgcggat ctacagtaat gagcaacagg tagcatcgca ccgtctctgt
     1021 tcggctgcat acggctggca gaccgtgccg gagcatcacg ctccactctg gcagcaggct
     1081 tcggagcgca tggccgaacg gctgggggag atacagaaac gggtaatcac tgtctgtgac
     1141 cgggaagcgg atatctggca ttacctgtat tataaagtca gccacggaca gcgtggtgcg
     1201 tgctgcacag aatcgccggc tggaagaggc acccggcaag ctcttcgaac tgccggaagt
     1261 cctggcaacc gcaggaagcc acacgctgaa tgtgatgcaa aaaggcgggc gggcagcccg
     1321 tcaggcccgg atgttcatca gttacagcga agtcagcata aaaaatcccg acaacagcgg
     1381 ccaggcgctc ccgctcacgt atgtctgttg ccgggagcag gcagaggacg gtgcctgctg
     1441 gcatctgctg acgtcaggaa aagcggcgag tgccgccgat gcacgacgta ttgtcagcca
     1501 ttacgagcga cgctggctga cagaggaata ccacaaggcg tggaaaagtg gtggtacatg
     1561 gaatcgctgc gaatgcagac ccgggataac cttgagcgca tggtggttat ccaggcgttt
     1621 atagcggtac gggtgctggg cctgcggcag ggaggtgtca gtgaagaaac acaaaatgac
     1681 agctgtgaga aaatactgac gccgacagag tggaaactac tgtgggttaa gctggaagga
     1741 aagccgcttc cggtgcaggc accgaccctg aaatgggcct ggggcgatgg catgacagca
     1801 aacgcacagg tcgtcccggt tggggcgtca tgtgggatgg ctggtcagac ttcaggatat
     1861 ggttgagggt tacctggtga tgaagtctct tgatcaggag ctctgatcaa gagacagcag
     1921 cttagggggg
//
LOCUS       DAJTXY010000258         1879 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTXY010000258 DAJTXY010000000
VERSION     DAJTXY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.263"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..1480
                     /locus_tag="OO566_004214"
     CDS             <1..1480
                     /locus_tag="OO566_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6607230.1"
                     /translation="FAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIVEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIADINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            1488..>1879
                     /locus_tag="OO566_004215"
     CDS             1488..>1879
                     /locus_tag="OO566_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607231.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTG"
BASE COUNT          521 a          368 c          458 g          532 t
ORIGIN      
        1 gtttgcgttc attattatcg tgttgctggt ggtatatttc atgaccactc gtgtgttccg
       61 tcggcaattt tctgaaatga ccgagctggt taatacgctg gcgtttttgc ccgactcaac
      121 ggatcagatc gaggctctga aaattcgcga aggcgatgcg aaagagatta tcagcatcaa
      181 aaattcgatc gtggaaatga aagatgccga aattgaacgg tcaaataaat tgctctcact
      241 gatctcttac gatcaggaaa gcggttttat taaaaatatg gcgattattg agtccaacaa
      301 taatcagtat ctggctgtgg ggatcatcaa actgtgtggt ctggaagccg tggaagcggt
      361 gtttggtgtt gatgaacgca ataaaatcgt caggaaattg tgtcagcgaa ttgccgagaa
      421 atatgcgcaa tgctgcgata tcgtgacatt caatgccgat ctctatttac ttctgtgtcg
      481 ggaaaatgta cagacattta cccgtaaaat agcgatggta aacgattttg acagcagctt
      541 tggctaccgc aatctgcgca tccataagtc tgccatttgt gaacctttgc agggggaaaa
      601 cgcctggagt tacgcagaaa aactgaaact ggcgatttcc agtatccgtg accatatgtt
      661 ctccgagttt attttctgtg atgacgcgaa actcaacgaa atagaagaga atatctggat
      721 tgcgcgtaat attcgccatg caatggaaat tggcgaacta ttcctcgtct atcaaccgat
      781 cgctgatatt aacacccgcg ccattctgag cgcggaggcg ttgtgccgtt gggtttctgc
      841 ggagcggggg atcatttcac cgctgaagtt cattaccatt gctgaagata tcgggtttat
      901 caatgagctg ggttatcaga ttattaaaac ggcaatgggt gaattcagac attttagtca
      961 acgtgcgtcg ctgaaggatg atttcttact gcatattaat gtttcgccct ggcagttaaa
     1021 cgaaccgcac tttcatgagc gttttaccac catcatgaaa gaaaatggcc tgaaggcgaa
     1081 cagcctctgt gttgagatca ctgaaaccgt gatcgagcga attaatgaac atttttatct
     1141 caatattgaa caactgcgta aacaaggggt acggatatcg attgatgact ttggcaccgg
     1201 tttgtcaaac ctgaaacgtt tttatgaaat taatccagac agcataaagg tggactcgca
     1261 atttaccggc gatattttcg gtactgcggg aaaaattgtg cgcattattt tcgatctggc
     1321 acgctataac cggatcccgg tgattgcgga aggcgtagag agcgaagacg ttgcgcgcga
     1381 attaattaaa ttaggatgcg ttcaggctca ggggtatctg taccagaaac ccatgccatt
     1441 ctccgcctgg gataaaagtg gaaaattagt aaaagagtag tttacgtatg tccagaatca
     1501 ataagttcgt acttacagtc agtctgctga tttttatcat gatttcagca gttgcctgcg
     1561 ggatctacac tcaaatggta aaggaacggg tgtatagcct gaaacagtcc gttattgata
     1621 ctgcttttgc ggtggcaaat attgctgaat atcggcgtag cgtggcaatt gatcttatca
     1681 acacgctaaa tcccacggag gaacagctgt tggttggttt gcgcacagct tacgccgact
     1741 cggtttcccc ctcttatttg tacgatgtcg gtccttatct gatttccagt gacgaatgta
     1801 ttcaggtaaa ggagttcgag aaaaattatt gtgcagatat tatgcaggtt gtgaagtatc
     1861 gacatgtcaa aaatacagg
//
LOCUS       DAJTXY010000259         1837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTXY010000259 DAJTXY010000000
VERSION     DAJTXY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.264"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..224
                     /locus_tag="OO566_004216"
     CDS             <1..224
                     /locus_tag="OO566_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607232.1"
                     /translation="IANFIALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGV
                     GPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            complement(866..1138)
                     /locus_tag="OO566_004217"
     CDS             complement(866..1138)
                     /locus_tag="OO566_004217"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607233.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASGSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            1227..1406
                     /locus_tag="OO566_004218"
     CDS             1227..1406
                     /locus_tag="OO566_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607234.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGISAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            1458..1652
                     /locus_tag="OO566_004219"
     CDS             1458..1652
                     /locus_tag="OO566_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607235.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
BASE COUNT          583 a          423 c          360 g          471 t
ORIGIN      
        1 caatagccaa ttttatcgca ttacaaataa atggttcaaa aacaggattt gccgcatcta
       61 ttacaggttt tactcaaaca ataagcgctg gtacaatcag tttcctgtca gtaaccgtaa
      121 gcgacaacgg tgttggtccc gggactactt taggtcttgg ttcactaata acttcattac
      181 tgtcaatagc cgtaatcgcc attgcaggaa ataataaaaa gtaagtgtac tctgactgcg
      241 tatgtatcta ctttgtgtaa tgcaggtgtc tattgaaaat aattcttttc gtgggcatct
      301 gttgtataaa ataaaaaatt atcgtaagta catctatctg cgatgtcttg tgaaaattat
      361 cctgtctggc aacaatcgcg tccatctgta ttaatggacg cgaacgatga actctcaaac
      421 aacactagat atcaccggct ttcgcactta tttgccagaa tcattacgtc tcagattcga
      481 cgagcaatta tctgtccggg ctatcgcaca gcagttaaga ctcagttatt cccccattca
      541 caatctcttt caacgaatta acgcctcagg tattgtctga ccatgacccg agtccctttc
      601 attcgcccaa ccgataccat actttatgcc aacagaaaga atgaatgaac cgcgcctaaa
      661 atcagcaagg acacatggca caaagtgcga catgtctcac atttaacccc agtgggggca
      721 acaagtataa aaccgttttt tgattaagtg gtcgaatatg aaaaggggaa ggtttatgtg
      781 aaaaatacct gtgttttctt tgaaaacaca caacagtatc aggttattta tgtttataca
      841 gtgcgttcca aactgacctg aataattacg caagttgcaa caataaaaca tttttgccgt
      901 agtgtgaggt actccggtaa attgctttcc aggtgcagac aatgactaac gaagaactac
      961 aggcaatcgc attatccatg cttgagcgcc agcgcgaaaa agtacaccag caacgagagt
     1021 ctttgcttaa cccatctcca ttcaccaggc caaaacgatt ggagcctgaa gcattcttgg
     1081 acaaataccc acggcggctt aaatcaggca aaaccagacc accaggctga tagctcacag
     1141 tttgttctcc gattcaataa aacggataag aaccatcagc gcggtagcgt aaggcactac
     1201 agcgcattgc tttacgagta ataacaatgg atttatcacc agaagacgca aagtttttac
     1261 gagaactgca acaccgtatg ctcagagaag ccattatcga acagcaaacg ggcataagcg
     1321 ccatagaccc attgcgatac aaacgggcct catctccttt agttgacgac aattccaatg
     1381 cggataaaac acgtccacca cactgattac atttttcatt agcacagtgg tgtgatgcag
     1441 catacgagga aaaccggatg aatgagaaca aaataaaaag gctggagcaa ctgttacagg
     1501 cacgccaacg ggagtttgcc actaaatggg gacgtgccgc gctgccacat gaacgcctaa
     1561 cctattttcc acttgaagac ctcattaaaa ataatggcaa aaaaatcagg tctgataaga
     1621 aacacttctt agaatgcaat gaagacaaat aacaacaatc tacatattgc caccaaccac
     1681 agatgtggca tcgttatcag cgcacttgat tttctttata tccactgacg agcttgccgg
     1741 ggcacctttg tttgccggtc attctggtat catgcagata tttacggcgt acaagcccac
     1801 tagcatgaca ttgtaataag gcctgagcct aacgctt
//
LOCUS       DAJTXY010000260         1805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTXY010000260 DAJTXY010000000
VERSION     DAJTXY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.265"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..786)
                     /locus_tag="OO566_004220"
     CDS             complement(<1..786)
                     /locus_tag="OO566_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6607236.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSE"
     gene            1134..>1805
                     /gene="rluF"
                     /locus_tag="OO566_004221"
     CDS             1134..>1805
                     /gene="rluF"
                     /locus_tag="OO566_004221"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6607237.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDL"
BASE COUNT          434 a          465 c          449 g          457 t
ORIGIN      
        1 ttcgctcata ttcgtttcaa ccattgcgcc gtgaatatca aaaaagcgcg agcaaatatc
       61 gccagcaacc tggcgggcat tcaggtcgtc actctcttca ccaccataaa acgcatgcca
      121 gttagcgccg tcgcgaatgg ccggtgagcc aattcccacc agggcgacat ccagattatc
      181 ccagtaggca gagatggttt taaagtgctg agactgcatg atcccattac gaattaatgg
      241 gttatccagc agagccggaa aatccgcgag atgcgattcc cctttcagct tcgctgccgc
      301 gctgtaggtt aatgtgttta catgatagcg gctttcgagt ttaccggacg gtccgccaat
      361 aattggtacg cagattaact gccgcgattg ccccgcctgc ggcaagtttt caaccagtgc
      421 gctcaccgcg cgaccccagg aaaaaccgac aatatcgcca ggctccagca agcgatccag
      481 cagttgcgcg ccgtgtaacc ccatcatcgc cagttgagtc tcttcatcct catcatttcc
      541 cgacaccacc acaacgtctt tcaggccaaa cttttgcttc agttgctgct ccagccagag
      601 attttcgttg tagtcatagt tgatggcgat ggtgaccatt ccctgatcgc ggcctcgttt
      661 aagcaagcgg ctgatggtgg tgcggtaaat acccagttcg cgcgcgattt gcgcctgcgt
      721 catatcctgt tcgtaataga gttgggcaat cttcacaatc aaacggatat cgtcactgtt
      781 ttccattgcc gcctcctggt tctgcacatt tgtgcaaata cgctttctca ccgcttgatc
      841 tgatataacc ccatcatgag ggcctttcaa agcgattttt cactctttgc atggggagaa
      901 atagcgcttt ctcttgcaca tttgtttctc tgcgtagtag tcatcagcac aggtgtgcag
      961 cgattcgtaa tatgttgatt atccttattt tgccaggcaa atttgcatcg tacttccgga
     1021 gacacttttg tgataaaagg cgtggcagat cacatttaat ttcgacgttg ccgcataacc
     1081 ctgtttttcc ccgctaagag gtacggtata ttccgcgttt tacaggagaa tttatgctgc
     1141 ccgactcatc agtccgttta aataaataca tcagcgaaag cggaatttgc tcacgccgcg
     1201 aagcggatcg ctatatcgag caaggcaatg tgttccttaa tggcaagcga gccaccattg
     1261 gcgatcaggt gaaacccggc gacattgtga aagtaaacgg tcagttgatt gagcctcggg
     1321 aagccgaaga tttggtactt atcgccctga acaagcccgt cggtattgtc agcaccaccg
     1381 aagatggcga gcgcgataac attgtcgatt tcgttaacca cagcaaacgc gtgttcccga
     1441 ttggccgcct ggataaagac tcccaggggc tgattttcct caccaatcac ggcgatctgg
     1501 tgaataagat cctccgtgcg ggtaacgatc atgagaaaga gtatctggtg acggtcgata
     1561 aaccgattac cgatgaattt attcgtggta tgggggcggg ggtgccgatc ctcgggacgg
     1621 tgacgaaaaa gtgcaaagtc aaaaaagaag cgccgtttgt cttccgcatt accctagtgc
     1681 aggggctgaa ccgtcagatc cggcgcatgt gcgagcattt cggctatgaa gtgaaaaagc
     1741 tggaacgtac gcgcattatg aacgtcagct taagcggcat tccgctgggg gagtggcgcg
     1801 attta
//
LOCUS       DAJTXY010000261         1773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTXY010000261 DAJTXY010000000
VERSION     DAJTXY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.266"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            396..>1773
                     /gene="pykF"
                     /locus_tag="OO566_004222"
     CDS             396..>1773
                     /gene="pykF"
                     /locus_tag="OO566_004222"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6607238.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPS"
BASE COUNT          479 a          439 c          423 g          432 t
ORIGIN      
        1 ctgacaactt cggcaccaga cgttgcgcaa acagcgaagt ttttgcgtaa ccttttccct
       61 ggaacgttaa atctttgata acaatttatt gtctaacaag ttgtatattt tttgaaacgc
      121 tgtttttgtt ttccttttgg attaatttca gcgtataatg cgcgccaatt gactcttgaa
      181 tggtttcagc actttggact gtagaactca acgactcaaa aacaggcact cacgttgggc
      241 tgagacacaa gcacacattc ctctgcacgc tttttcgatg tcacctatcc ttagagcgag
      301 gcaccaccac tttcgtaata ccggattcgc tttccggcag tgcgcccaga aagcaagttt
      361 ctcccatcct tctcaactta aagactaaga ctgtcatgaa aaagaccaaa attgtttgca
      421 ccatcggacc gaaaaccgaa tctgaagaga tgttagctaa aatgctggac gctggcatga
      481 acgttatgcg tctgaacttc tctcatggtg actatgcaga acacggtcag cgcattcaga
      541 atctgcgcaa cgtgatgagc aaaactggta aaaccgccgc tatcctgctt gataccaaag
      601 gtccggaaat ccgcaccatg aaactggaag gcggtaacga tgtttctctg aaagctggtc
      661 agacctttac tttcaccact gataaatctg ttatcggcaa cagcgaaatg gttgcggtaa
      721 cgtatgaagg tttcactact gacctgtctg ttggcaacac cgtactggtt gacgatggtc
      781 tgatcggtat ggaagttacc gccattgaag gtaacaaagt tatctgtaaa gtgctgaaca
      841 acggtgacct gggcgaaaac aaaggtgtga acctgcctgg cgtttccatt gctctgccag
      901 cactggctga aaaagacaaa caggacctga tctttggttg cgaaaaaggc gtagactttg
      961 ttgctgcttc ctttattcgt aagcgttctg acgttatcga aatccgtgag cacctgaaag
     1021 cgcacggcgg cgaaaacatc cacatcatct ccaaaatcga aaaccaggaa ggcctcaaca
     1081 acttcgacga aatcctcgaa gcctctgacg gcatcatggt tgcgcgtggc gacctgggtg
     1141 tagaaatccc ggtagaagaa gttatcttcg cccagaagat gatgatcgaa aaatgtatcc
     1201 gtgcacgtaa agtcgttatc actgcgaccc agatgctgga ttccatgatc aaaaacccac
     1261 gcccgactcg cgcagaagcc ggtgacgttg caaacgccat cctcgacggt actgacgcag
     1321 tgatgctgtc tggtgaatcc gcaaaaggta aatacccgct ggaagcggtt tctatcatgg
     1381 cgaccatctg cgaacgtacc gaccgcgtga tgaacagccg tctcgagttc aacaatgaca
     1441 accgtaaact gcgcatcacc gaagcggtat gccgtggtgc cgttgaaact gctgaaaaac
     1501 tggatgctcc gctgatcgtg gttgctaccc agggcggtaa atctgctcgc gcagtacgta
     1561 aatacttccc ggatgccacc atcctggcac tgaccaccaa cgaaaaaacg gctcatcagt
     1621 tggtactgag caaaggcgtt gtgccgcagc tggttaaaga gatcacttct actgatgatt
     1681 tctaccgtct gggtaaagaa ctggctctgc agagcggtct ggcacacaaa ggtgacgtcg
     1741 tagttatggt ttctggtgca ctggtaccga gcg
//
LOCUS       DAJTXY010000262         1745 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTXY010000262 DAJTXY010000000
VERSION     DAJTXY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.267"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(142..1356)
                     /locus_tag="OO566_004223"
     CDS             complement(142..1356)
                     /locus_tag="OO566_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6607239.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          523 a          424 c          361 g          437 t
ORIGIN      
        1 cagggtcact gtctgaatac gacgaatccg ttgcggttta ttacccgcaa cactggttga
       61 cacatcaacg tccattttta gctccttgag cggaattaat cgatttgatg actattcaga
      121 aaataattaa gcgaaccgtt attaccagtt caacaatgtg ccatcttcca gtcgtgcgac
      181 ggggagatac tccgggtcat acggatattt cgccgccagt ttctcatcga aagaaatacc
      241 cagccccggt tcatcgcctg ggtgcatata accttcctcg aagcgccagc tgtgcgggaa
      301 aacttcaagc atttgttcgg aatagcccat gtactcctga acgccaaaat ttggtaccca
      361 taggtcaaaa tgcagcgccg ccgcatgaca aacaggcgat aaatctgaag ggccatgcga
      421 accggtacgt acctgataaa gcgatgcaaa atcggcaatc cgccgcatcc cggtaatgcc
      481 gcctgcatgg gtgatcgtcg tgcgaatata gccaatcagc tgctcttcga ttaactgctt
      541 gcagtcccag atactgttaa acacttcacc gaccgcaatc ggcgtcacgg tatgctggcg
      601 aatcagacga aaacattcct ggttctcggc aggcgtcgga tcttccatcc agaacaggcg
      661 gtaatcctca atgctcttcc caaagcgcgc cgcttcgatg ggggtcaggc gatggtgcat
      721 gtcatgcaac agatgttcgt taaaaccgaa cttattacgc accgcttcga ataacttcgg
      781 cgtaaaatcg agatactttt ctgaagacca caactgctct tctggccaca ggcctttggt
      841 tgccggttca tacgccagtc ctttgccctt cgccaggcca taagtggttt gcatacccgg
      901 tacaccgcac tgcacacgaa tcgccttaaa gcccatctgc tggtgctttg catagtcttc
      961 aaggacttca tcaatcgtac gcccggtggt gtggcaataa accatcacgc cttcacgaga
     1021 agcaccacca agcagttgat acaatggcat atttgccgct ttagctttga tatcccacaa
     1081 cgccatatca atggcagaaa tggctgacat cgtgacgggg ccacgtcgcc agtaagcacc
     1141 tttataaaag aattgccaga tatcttcgat acggctggca tcgcggccaa ttagttgtgg
     1201 acaaagatga tctttcagat aagaagcgac cgacaattcg cgaccattta acgtggcatc
     1261 tcctaatcca caaagtccgc tttcagtggt tattttaagc gtaacgaaat tacgccctgg
     1321 gcaagtaaca aaaacatctg cagcaataat tttcattatt gcaatctcct gtgttgtaaa
     1381 tagacgtttc aaacctaatt ttacgcctac taccatacaa gagagaaaaa gcgattattc
     1441 aaccgtgatc acattaaaaa caaaaatttc caccgaggag aaaaaatacc ctcaaaaacc
     1501 acctgaataa ttaaccatta ttgttaaaac aacacgttaa ataatcacat aacaaactcc
     1561 attaatcatt gaaaatgagc acagaaatag aaattaatta tattcgatta attaaaatgt
     1621 gtattgcttg atgtatttaa aaatattatt ttcatggtga gagtggaggt aaagaagaca
     1681 caataataaa aaggaatgtg acaaataatg gttctggttg ggcgttaaat ttaaggtcac
     1741 cagaa
//
LOCUS       DAJTXY010000263         1686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTXY010000263 DAJTXY010000000
VERSION     DAJTXY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.268"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..273)
                     /locus_tag="OO566_004224"
     CDS             complement(<1..273)
                     /locus_tag="OO566_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6607240.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNR"
     gene            411..1331
                     /locus_tag="OO566_004225"
     CDS             411..1331
                     /locus_tag="OO566_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6607241.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1331..>1686
                     /locus_tag="OO566_004226"
     CDS             1331..>1686
                     /locus_tag="OO566_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6607242.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGCP"
BASE COUNT          458 a          395 c          418 g          415 t
ORIGIN      
        1 gcggttctgc agatcgctca gggatgtcag ggcagaatct atgtcatgca atatagggcc
       61 aagaacagac aaaagatgct cgcccgcttc agtgggtaca acgctgcgcg tggttcgcgt
      121 cagaagccgc aatcccaatc gttcttctat acggcgcact atctggctta aagctgactg
      181 caccatgctc aggcgggctg ctgcgcgagt gaagctacgc tcctctgcaa cgacgacaaa
      241 cgccatcaga tcagcgattt cttcacgctt cattcagtat ctccatacaa tctattaatc
      301 atttaaatga taggtctaat catgatttag ggtctaatca acttaatgat caatatctat
      361 agtgatatca ccaaccgtca aaacgaaccg acaactccat gaggaactga atgatgaata
      421 ataaagtcag cttcactaac agcaataatc caaccatctc tttgtctgca gtgatctatt
      481 tccctccaaa atttgatgaa acccgtcagt atccggcaat tgtggtatct cacccaggtg
      541 ggggcgttaa agagcaaacg gccggaacct atgccgaaaa gctggcggaa aagggatttg
      601 tcactattgc ttatgacgca tcttatcaag gtgaaagtgg cggcgagccg cgtcagttag
      661 aaaacccata cattcgtact gaagacgtca gtgccgtgat tgattacctg acaacgcttt
      721 cttatgtcga caatacccga attggtgcga tgggaatttg tgccggtgca ggatatacag
      781 ccaatgctgc aattcaggat cgccgcatca aggccatcgg taccgttagt gcggtcaata
      841 ttggttcgat gttccgcaat ggttgggaaa acaatgtgaa atctattgat gctctgccac
      901 acattgaagc tggctcaaac gcccgaacca gcgatattag cagcgaagaa tacgccatta
      961 tgccactggc tccaatgaag gaggccgatg cgccgaacga agaactgcgt caggcctggg
     1021 agtattacca cacacctcgt gcgcagtatc caacagcacc gggttacgct actttgcgca
     1081 gccttaacca gattattacc tatgatgctt accatatggc ggaagtgtac ctgactcagc
     1141 cgatgcagat tgtggcgggc agccaggcag ggagcaaatg gatgagtgac gacctgtacg
     1201 atcgggcatc aagccaggat aaacgctatc acattgttga aggcgcaaac catatggatc
     1261 tttatgacgg caaagcctat gtggctgaag caatttcagt attagctccg ttctttgagg
     1321 aaactctgta atgaaacata ttcaaatccg caatagtgat atggcctggc atattgctgc
     1381 caatattcag tttccaccaa attttgatga gtcaaaacag tatccggcga ttatcagcgt
     1441 ccatcctttt ggcagttgta aggagcagac gtctggcaat atctatggca aggcattggc
     1501 tgaaaaaggc tatgtcgttc tcgcttatga cgccagtttc cagggagaat ctggtgggga
     1561 gcctcgttgg atagaagatc cgacacagcg cgtagaggat attagccgtg ttatcgatta
     1621 tgcagtaact ctgccatatg ttgatgctga acgcattggc gtgctgggtg tgtgtggggg
     1681 gtgccc
//
LOCUS       DAJTXY010000264         1668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTXY010000264 DAJTXY010000000
VERSION     DAJTXY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.269"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(71..538)
                     /locus_tag="OO566_004227"
     CDS             complement(71..538)
                     /locus_tag="OO566_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6607243.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(604..843)
                     /locus_tag="OO566_004228"
     CDS             complement(604..843)
                     /locus_tag="OO566_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6607244.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(978..1583)
                     /locus_tag="OO566_004229"
     CDS             complement(978..1583)
                     /locus_tag="OO566_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6607245.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          536 a          369 c          291 g          472 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaagcat ccgatgattc atttatgctg
       61 tcctaaaaaa ttaacaaaac aatgggaaaa caggatatgt aagcagaaaa gccaatgaaa
      121 ttggtacagc ataaggcaca ccgttttcac gaattgattt tcgaaaaaaa agaagacctg
      181 ccagaacaac cagagcgccg caaaaggtca ttgcgacgag aaaattagcc agttcatgtt
      241 catcaatagc taacgaaaga acagttaaca gcttgacatc accgccgcca attatccctg
      301 cggaaaaaag aaagaagccc acgagcaaaa cgctcccggc tgcaaccaga ttgggttttt
      361 gatgctgtaa aatgagtgcc aaaaaaataa gcacggttaa caccagtaaa taacgatggc
      421 taataattct gagtaatata tctccccatg aaataaaaat aagaaagagc gcaacaaata
      481 aaagatcgac tcttagtaac catattgctg ccattgaaag actccatgta ccatacaagg
      541 ttgctgccag acatattcct ttacgtctga cagcctctag agagtttttt tattttttat
      601 aagttacgga ttactcgttc cggaaatcgt tacctgttga attgctgcag cgatagcgtc
      661 gaatgcatct ttaattgctc ccaggaagcc tgagttctga tcgccaaaga tgaaagcaag
      721 cagagttgcc atagcaacac caatcaatgc atattcgatt gcagtaacac cacgttcatc
      781 cttcgcgaaa cgcgcaaagc actgtttagt tttaccatag tacttagtaa taaaagtatt
      841 catgttttcc tcgtatatta ttatttaaat tcaaaccacc agcttcatct gcgtgtcacc
      901 ggtatcccat atttttggga tgtggctata attatccatt cccgctattt agtcttcaaa
      961 aaaaattaca tttaatatca tttatcttga ttaagaaata gtaagactaa aaaaacaagc
     1021 gtcgtcagcc agcacactgc taacacggta agaacgctaa taaaaacact ccactgcttc
     1081 gttctatacc attcaatatt tttgtcagta tgttttattg ggagcagctc ttcagtagag
     1141 aattcctggc tgttatcatt ctcaggaatg tttgcatcag tgtttacttc cctggcctcg
     1201 ctatcgttgg tatagctact actgtcttca tcactgtcac gctcattctc acagacatgt
     1261 ttctcgacct ttcccagata tctgacgcct tttcgcggaa tggttttaat tagagaactc
     1321 gacaaacctg cgacgtctaa tgcctttcta agcatgtaaa tctgcccata ataactgctg
     1381 tcgcttaccg aacctttctg ctcccgccaa acctcattga taatatgttg cttatcggat
     1441 tctccctgta gtaaaaggag taaaaatcga tagccgtttt cagacagatt gattacattg
     1501 cctgtactgg tggtgatacg tcgagacgct gggtaaaact taactccctg ccctaaagaa
     1561 aaataaaaac ggtcatcgct cataagtacc gaaccctgct ttcttcttca catgactact
     1621 cattacaact ttgtatgaag taagatgcaa aatcatattg tttacatt
//
LOCUS       DAJTXY010000265         1625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTXY010000265 DAJTXY010000000
VERSION     DAJTXY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.270"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..715)
                     /locus_tag="OO566_004230"
     CDS             complement(<1..715)
                     /locus_tag="OO566_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6607246.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGP"
     gene            complement(1031..>1625)
                     /locus_tag="OO566_004231"
     CDS             complement(1031..>1625)
                     /locus_tag="OO566_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6607247.1"
                     /translation="WWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHLDIGVGTGFYLT
                     HVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALHGQFDSISMFYL
                     LHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFS
                     NTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          469 a          301 c          348 g          507 t
ORIGIN      
        1 gtgggccgtg gtagtccggt tgggcggttt gttgtaacaa agtttgcatt attctgggag
       61 atagttgatt ttctctaaga ttgacaacgc tatcagaact gaggccaagt atgctttccg
      121 gaattgccgt gatccggttc tgattaagcc agaatattct tataatatga tcatctcttt
      181 ctggaaactc tggtattact tccaaagcat ttctggctgc atcaagtaat tccaatgaca
      241 ttggtaacct cggaagtaca gacaggtgat tgtcacttac attaatatat tctagtgacg
      301 caggtaattc aggaagtgcg aacaaatggt tatcactcac atttatatat tccagcgaca
      361 ctggtaattc aggaagtgca gataattgat tactgcttgc attcagctct ttcaatgccc
      421 ttggtagctc ggggagcatt gatagttggt tattgcttac attaatttca tcaagattgt
      481 caggcaatcg tggtagagat ctgagaccta aacaagataa gtccaacgat gtttcactgt
      541 tctccagaca taattggagc cgggtaaaag cagtttccct gttttctccg gaaatgctgt
      601 ttttagtcca ttcaacccat tcggagagat aattattgtg aacattgtcg attgatgtag
      661 ttctgtaaaa agagacgttt ccagtggata gggggggatt atttacagga agcataataa
      721 cctcgcagaa ggatatcctg ataaaatgtg atactgggga ataataagct gaaataaaat
      781 gtttcaggat tgaaatttat tgttttcatt ttaaggaagt gatgttgatt taactgtaaa
      841 aaaacgatac tattttctta gatggtaaat gtctcgcctg tgctatattt tgtttttcgg
      901 ttagttaaat atcgtacgtt catatttgaa cgttctgccg gaatgcatta tcaatagagg
      961 taaagtcgca accccaaatc gtaaaggaaa ccgtagcacg tcttaagcaa gaacgtgctg
     1021 cggttggatg ctattttttc cctgaagcgg aaaacattac tacagtacct tgaaccttgg
     1081 ttttaacatt ctcgaaatgc tctgagagta tatgtgttaa gccttcttcg gaatcttttg
     1141 tgtttgaaaa gatgcctttc tgattgtaaa tgcgcatcag tttttgaccg aagctattgt
     1201 gcacaactcc atcgccaaga attgtggctc cgtatagagt tccatcatca gttaaggcct
     1261 gcgccgcatt gcgtattaca cagctttttg tagatatatt tccaggcagg cagtgaagaa
     1321 ggtaaaacat ggaaatggaa tcaaattgac catgtaacgc cgcaggataa ggttcaaaaa
     1381 catcatggct aattttatgt ttaatttttg attccccagc ccttgtagat gccgcgttca
     1441 ggctagcttc gttcaaatcc attaaagata tcagactact ctcaggtacg tgagtaaggt
     1501 aaaacccagt tccaacacca atatccagat ggttgttacc taaatgttcc agaaagtgtg
     1561 gaagaaggtg ttcctttgta ggacatcccc atgcaagccg atttgatact cccaaaaccc
     1621 accag
//
LOCUS       DAJTXY010000266         1595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTXY010000266 DAJTXY010000000
VERSION     DAJTXY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.271"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..122
                     /gene="kil"
                     /locus_tag="OO566_004232"
     CDS             <1..122
                     /gene="kil"
                     /locus_tag="OO566_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6607248.1"
                     /translation="NIKKRKLYIRSLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            122..292
                     /locus_tag="OO566_004233"
     CDS             122..292
                     /locus_tag="OO566_004233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607249.1"
                     /translation="MTKKIKCAYHLYNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            367..642
                     /locus_tag="OO566_004234"
     CDS             367..642
                     /locus_tag="OO566_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607250.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            742..>1595
                     /locus_tag="OO566_004235"
     CDS             742..>1595
                     /locus_tag="OO566_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6607251.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLA"
BASE COUNT          507 a          353 c          360 g          375 t
ORIGIN      
        1 acaatattaa aaagcgaaaa ctttatattc gtagcctgac tacaaaaaca tgcatttctg
       61 actgcatgat taaagtcttc ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt
      121 aatgactaag aaaataaaat gtgcttatca cctttacaat aaagaaattg aagaaagcaa
      181 aatcattaca agaccacttc atttcatgcg tggagttata ccaacgacgg aaatgaaaaa
      241 atattgtagt gaaatctgtg ccgaaaaaga ccagatggca cacgaacttt aattaactga
      301 ctatccgaaa ctgaatttat gccagcaatg gcagggattc gctcaacctt aattaaggag
      361 aaaaacatga ttaccagtta tgaagccact gttgtaacta ctgatgacat tgttcacgag
      421 gttaatctgg aaggaaagcg tattggctac gtgattaaga cagaaaataa agaaacccca
      481 ttcactgtgg ttgatatcga cggtccatca ggcaacgtta aaacacttaa cgatggtgta
      541 aaaaaaatgt gtctggtgca cataggaaag aatctgcccg cagaaaaaaa agccgaattt
      601 ctggcaactc tgattgcaat gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac
      661 actgtgcagg cctgagtgaa gaacctggga catttattca tcactcgcag taattttaat
      721 ctgagttgag gttaaaaaac aatgaacacc gataaacaag tttacccact gtattacgaa
      781 gcaaaaaatg acaaagtaag aaaacgtctc ggtattaaag gcggttttta ctgggctgaa
      841 gcgaaaaaat tatccattgc catctcccgt ggtgctgttg cgattgacga tgcaggctac
      901 gatgaagatg actttaaaaa acctgttcgc gtcaatttgc ccgttgttga tgacctcccg
      961 ccagaaggcg tatttgatac ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc
     1021 atcacaatgg tatttatcgc atcttctccc tctgttcagg acaaaccggc cagcactgac
     1081 aataccaacg tcaacggcga agacatgaca gaaattgagg agaacatgct cctgccagtt
     1141 tccggtcagg aactgcccat tcgctggctt gcgcaacacg gcagcgaaaa accggtaacg
     1201 cacgtttcac gcgacgaact ccaggcatta cacattgctc gggatgaaga actaccagct
     1261 gttactgccc tagctgtttc ccacaaaacc agcctgctcg atccgctgga aattcgcgat
     1321 ctccacaaac tggtgcgtga tactgacaga gttttcccta atccaggcaa ttcaagcctg
     1381 gggctgatga ctgctttttt cgaagcatac atggacgcag actacaccga tcgcggtctg
     1441 ctgacaaaag agtggatgaa aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc
     1501 gctaatgctg gcggcgggaa cctcaccgat cgcggcgaag gtttcgttca cgatctgacg
     1561 tcactggcgc gcgacgtagc cactggcgta ctggc
//
LOCUS       DAJTXY010000267         1589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTXY010000267 DAJTXY010000000
VERSION     DAJTXY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.272"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..265)
                     /locus_tag="OO566_004236"
     CDS             complement(<1..265)
                     /locus_tag="OO566_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6607252.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMF"
     gene            complement(330..1280)
                     /gene="virK"
                     /locus_tag="OO566_004237"
     CDS             complement(330..1280)
                     /gene="virK"
                     /locus_tag="OO566_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6607253.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            complement(1285..>1589)
                     /gene="capU"
                     /locus_tag="OO566_004238"
     CDS             complement(1285..>1589)
                     /gene="capU"
                     /locus_tag="OO566_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6607254.1"
                     /translation="SLVVLPSENESFGMVLAEASAFSVPVVATQIGGIPEVIQNNQTG
                     TLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAKQK"
BASE COUNT          485 a          350 c          299 g          455 t
ORIGIN      
        1 caaacatttt gtcgacaatt atttcccgtt ctgtatcact cttttctggg aaacaaagcg
       61 ataaattaat cgtcgccctg cgacgagctt ttttgctcaa tctgcccgcc caacgcccta
      121 tttttgccaa gacaggatct ctgaatgaag ggggaaacat tgaaatacct gcaatcattc
      181 ccaaaaggaa ccatgtagac cagtaaacag gggaaaggta atttttctta aattcaggaa
      241 taaactcaga tttatatttt ttcattattt agtgcgaatg cctctaccaa aattatcatt
      301 actgttttat tcagagaata catcaaacct taatttaaat tctgatgttc tgagtgtaca
      361 ggatatgcat ccaaagagcg gatagtagtt tcatggatat aatccagcaa agcgtatcgt
      421 tttcggtact cagagcgttt tttactggct atatcgctta gcggctttcg aatcacctgg
      481 gttggtaatc tgtaccagtc accacaggtt tttcctgcta cagactccca gaaatcacta
      541 tagaccgcaa caaaggtttt gcgtttctga taccagtatc tcaattgccg gaaaacatga
      601 ctgtgctcac tgactgccag acactcagag atattgcaag ctttcatcaa cgcacaaaat
      661 gcttcaaaaa taatccgttt tgggaaaatc ccataacagg cccgggttgc gcaccgaata
      721 acatcaggtc cggtattttt aggggctccc tgtaatccgc caataaaagc aatgtgtcca
      781 ttttgagtca gaatgactga aaatgttaac cgggcaataa ctgttttatt gtaaaacaat
      841 gacaacatca actctccttc ccggtcaaaa ccactgggag aacactggat atcaaaattc
      901 tctccatctt tcccgatgaa ttggactaat gataccgatt tttgtggaaa caaaaattta
      961 gaatattttt ctggcaaatt ttgcacaaag cgataatgct caagaatata ttgtccacgg
     1021 gtccaggcgc gccccccttt atgcaaataa gggcgatgaa ttcttgctgg caacgtgggg
     1081 tgtattgcca gaatatcctc aatgcacggc aaggaacgca gttcataata atatcgaata
     1141 ctgacaaccg gatttataga acatcgcaaa atgtacatga accgaaattt tctgtgttcc
     1201 cacttccccg gtagtgaatc tgaggaaaaa ataatccttt taaggaaacc gataaatgat
     1261 aagttactta tagaaaacat attgttactt ttgcttcgct aatgtgagta tttttaacgc
     1321 agttttatta atatcaaacc gctcttctat atcctgtttt gccaggcgag ccatctgata
     1381 aaaacgccca gggtcattaa aaaaatcatt cagggcgcac atccatgcgt gcttattact
     1441 tgctggtaac aatgtcccgg tctggttgtt ctgaataacc tcagggattc caccaatctg
     1501 agtggccact acaggcacag aaaatgccga tgcttctgcc agcaccatac caaaagattc
     1561 gttttctgaa ggcagaacca ccagactgg
//
LOCUS       DAJTXY010000268         1571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTXY010000268 DAJTXY010000000
VERSION     DAJTXY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.273"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..452
                     /locus_tag="OO566_004239"
     CDS             <1..452
                     /locus_tag="OO566_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR6607255.1"
                     /translation="HEARKNIPPKNPVDADKLLAASRGEFVEGISDPNDSKWIPGHHI
                     SSNEVKKTENEVRQTEDKQHQNSEPEEGTPCNQTGEDNCPDCGAVMGDATYQETFDEK
                     NQMKHRKKSRRKRKKLMICYRRTLAAISIMIAIMKLAKMNSTGKNRC"
     gene            567..863
                     /locus_tag="OO566_004240"
     CDS             567..863
                     /locus_tag="OO566_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR6607256.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            869..>1571
                     /locus_tag="OO566_004241"
     CDS             869..>1571
                     /locus_tag="OO566_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6607257.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSGWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFK"
BASE COUNT          489 a          346 c          400 g          336 t
ORIGIN      
        1 tccatgaagc ccggaaaaac attccgccca aaaatcctgt cgatgcagac aaattactgg
       61 ctgcctctcg cggagaattt gttgaaggga ttagcgaccc gaatgattcg aaatggattc
      121 ccggtcatca tatctcctca aatgaagtca aaaaaacgga aaatgaagtg cgacagactg
      181 aagataaaca gcaccagaac agcgaaccgg aggagggaac accctgcaat cagactggcg
      241 aggataactg ccctgactgt ggcgcggtga tgggagacgc aacttatcag gaaacttttg
      301 acgaaaaaaa ccagatgaag cacaggaaga agagccgaag aaaacggaaa aagctgatga
      361 tttgttaccg gagaacgctg gcagcgatca gcataatgat agcaataatg aaactggcga
      421 agatgaactc aactggaaaa aacaggtgtt gattgcggcg gtttacggtt tatgtgcgaa
      481 tcctgcgtgc atagcctatg cgcctgcaat ccctgacatc gcaatgatga ttgccaacaa
      541 gcttgaaaat tttggagtga ccagtgatga gcgcttaccc gatttttgat cgtattgaag
      601 aaatggcatg ggctcgccac taccagcaga tcgttcgtga agaaaaagaa acggaactgg
      661 cggacgacct ggaaaaaggt ctgcctcagc acctgtttga atcgctctgc atcgaccatt
      721 tgcaacgcca cggtgccaat aaacaggcaa tcagccacgc atttgatgat gatgttgagt
      781 ttcaggagcg catggcagaa cacatccggt acatggctga aaccattgcc caccaccagg
      841 ttgatattga tttggaggac taaaatctat ggcgcttttt caacgagcaa taaacacaca
      901 ggcctatcta aaagcaggaa ttatggggtt tgctggcgac ggcaaaacct acacagcaag
      961 cgaactggct attggtctgg taatgcttat gcggcaacgg ggaattgaag ctggtaaccg
     1021 cccggtaatg tttctggaca cagaaacagg ctctgactgg gttaaacccc gctttgatgc
     1081 ggaaaatatc gaattattta cggcaaaaac ccgctcgttt gttgatctgc ttgaagccat
     1141 taatgaagcg gaaagcagcg gttccgtaat gatcatcgac tccatcagtc acttctggac
     1201 cggattatgc gacgagtatg ccagacgccg aaaccgtaaa cgaggactgg aattttcagg
     1261 ctgggcgtgg ctcaaacagg agtggcgacg ctttaccgat cgatttgtta acagtcaggc
     1321 gcacattatt atgtgcgggc gtgcaggata tgagtatgac tttttcgaag gtgatgatgg
     1381 caaacgccag ttggcaaaaa ccggtataaa aatgaaagca gaaacagaaa ccggttatga
     1441 accctccatc ctgatccaga tggagaagca aatgaacata gaaaccggtc aggtgtggcg
     1501 caccgccaga atactaaaag atcgctccac ccgtattgat ggacaggtat tttcaaaccc
     1561 aacatttaaa a
//
LOCUS       DAJTXY010000269         1523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTXY010000269 DAJTXY010000000
VERSION     DAJTXY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.274"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..191
                     /locus_tag="OO566_004242"
     CDS             <1..191
                     /locus_tag="OO566_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6607258.1"
                     /translation="ENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLT
                     DEVLALRLSENGSQLHHS"
     gene            399..668
                     /locus_tag="OO566_004243"
     CDS             399..668
                     /locus_tag="OO566_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607259.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            665..891
                     /locus_tag="OO566_004244"
                     /pseudo
     CDS             665..891
                     /locus_tag="OO566_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            920..1510
                     /gene="ospG"
                     /locus_tag="OO566_004245"
     CDS             920..1510
                     /gene="ospG"
                     /locus_tag="OO566_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6607260.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          434 a          303 c          386 g          400 t
ORIGIN      
        1 tggagaatga gtaccctcag agggtggctg accggctgaa agcatcaggt ctgagcggtg
       61 atgcggatgc ggagagggaa gccggtgcac aggtgatgcg tgagactgaa cagcagattt
      121 accgtcagct gactgacgag gtactggccc tgcgattgtc tgaaaacggc tcacaactgc
      181 accattcata atcacatcgc ataaaactgt cagcgccagt gataggacag gtgaaacctg
      241 tcaatacgta ccggcttacg ccgggcttcg gcggttttac tcctctgcca tatgcaacaa
      301 cggagtgccg ccttacatgc cgctggcgcg gcatatcctg gtgacgatat ctgaacagtt
      361 atatactgtg tatatacgtg gtaatgacga ggtggcaaat ggcacaggtt aatatgagtg
      421 taagaatcga cgcagaactg aaggatgctt ttatggccgc tgcaaaaagc atggaccgta
      481 atggctctca gttaatccgg gattttatgc gccagaccgt tgaacggcag cacaattctt
      541 ggttccgtga ccaggttgcg gcaggccgtc agcaactcga atgcggtgat gtgcttcccc
      601 atgacatggt cgaatcctct gctgctgcat ggcgggatga aatgagcagg aagattgctg
      661 ataaatgatg gagatgttct ggaccatgct ggccatccag gacaggaagc gcattcgtag
      721 tacattgcgg agcagaacct gatggccgcc attgaaccgg acgaacggat tggttattca
      781 gccagcagcc tggcagggca gccgtataaa ggccgcaacg gtcgtgtgga aggaacatct
      841 ggaccacaca aggtagcgtg taacgttatt ttgtgtgaaa atctcttgtg atttttaaat
      901 atcgtaacaa aggtatgaca tgaaaataac atctaccatt attcaaacac cttttccatt
      961 tgagaataat aattctcatg ctggcatagt aacggagccc attctcggta agttaatagg
     1021 tcaggggtcg acagcagaaa tctttgaaga tgtgaatgat tcatctgctt tgtataaaaa
     1081 gtatgatctt attggcaacc agtacaatga gattctggaa atggcttggc aagaatctga
     1141 gctttttaat gctttttatg gcgatgaagc atccgttgtt atacagtatg gcggagatgt
     1201 gtacctccga atgctgcgcg tgcctgggac tccccttagt gacattgata cagctgatat
     1261 ccctgataat atagagagcc tttatctaca gttgatatgt aaattgaatg agttgagtat
     1321 aatccattac gatcttaata caggtaatat gctgtatgat aaagaaagtg aaagtttatt
     1381 cccaatagat tttcgcaata tttatgctga atattacgct gcaaccaaaa aagataaaga
     1441 gattatcgac cgacgattac aaatgcgtac aaatgatttt tattcgttat taaacaggaa
     1501 atatttatag ccgtatttgt tga
//
LOCUS       DAJTXY010000270         1502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTXY010000270 DAJTXY010000000
VERSION     DAJTXY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.275"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..1071)
                     /locus_tag="OO566_004246"
     CDS             complement(<1..1071)
                     /locus_tag="OO566_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6607261.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLS"
BASE COUNT          450 a          238 c          326 g          488 t
ORIGIN      
        1 ggaaaggcgg tcaaggaacg cggagaatgc ccgggaattc tcctcggtgg aaaaagaagt
       61 ccattgtgta gaaacctccc gacgatctgc ttcaggaaac caggctgcca cagtttcggg
      121 aagagggggg atctgaaagt tctgcccccc ttcagtgatg actctgatat ggcgcgaatg
      181 taaattttgc ggcaacatct ggcgtaaaaa cagtctgttt tggtctgaca gggaaatacg
      241 aaactgaggc ccgttaaaat ttggaaccga agtttgctgt attaagaata gcagagtttg
      301 atctgataag gggttatttg caatctcaat agttatatga ggatctaaac gaaaaagatt
      361 tgtcgggatt gtagttattt ggttgttttc aaaataaaaa aatcttctca tgataatatt
      421 ttcttggatg tttggtaagt tgcttaatct attgctatat gcaaaaagaa gttgcagtct
      481 cctaggtaaa gggggcagtt cagtaagatg gttgtgcgca accttaagta tcttaagatt
      541 agaaggcaga gatggaagag tttgcagttc gttgttccta acctcaagtt tttttaaatt
      601 caagggtaat gaaggcagtg tggataacct attatactct gcgcttagtg atttgatggt
      661 gtctggtaat aacggcagat tctctaattg attttcggat acatccaaag ttattagata
      721 atgtggtaat gcagatatat gtgttaagaa attatctctt gcaataagtt ctttaatatt
      781 ttcaggtaaa tcagtaagga tggttaattt attacagctc acatcaagct ttttaatgca
      841 agcagggagt acaggtaacg aggaaagcgc caaggctcgc aggtccagtt tatgcccctt
      901 agaggtaaga catgcacgaa gtcttcttac cgcctcagta cgctgttctt ccggtaaccc
      961 ctgtttctcc caatcatccc attcggacaa ataatggttg ggggatgtaa ttgtgctgat
     1021 atgtggaaat aaacaggatg aaatatttct gagcatatta atttctcgca tatggatatg
     1081 gaattagatt gtgaataaat tgtatttaat gtatctctcc taatgaggag ggtgtttcag
     1141 aatattcgcc ataagggaat gagtgttata tcatgctttc taccctggta agtatatttg
     1201 cttttttcgt atatttggtt catctttttt tgatgtttta catattcaca atgcagagga
     1261 tttataccag aagacattag tcttgagtaa gatactacaa catctccaac tattttagcc
     1321 agcctgacta cttgacttgg taatatttgt tgctaataat catgtataaa ataagcttat
     1381 atacatagta acttcagcaa aggatttagt aatgatccaa atcatatctc aatgtactgt
     1441 ttttttcttt aaaaaaacag tacattctat tttataatga ctgtgtattc ccatggaagg
     1501 ag
//
LOCUS       DAJTXY010000271         1499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTXY010000271 DAJTXY010000000
VERSION     DAJTXY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.276"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(1..264)
                     /locus_tag="OO566_004247"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="OO566_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO566_004248"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO566_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6607262.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1176..1499)
                     /locus_tag="OO566_004249"
                     /pseudo
     CDS             complement(1176..1499)
                     /locus_tag="OO566_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          418 a          379 c          260 g          442 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg catctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag ggctaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg cataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatcct
     1381 ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt cagtgtcctg
     1441 gcggtatcac ggccccctgc gccgttatgt gccatttcaa cgatctgctc cttaacgcc
//
LOCUS       DAJTXY010000272         1491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTXY010000272 DAJTXY010000000
VERSION     DAJTXY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.277"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            443..1192
                     /locus_tag="OO566_004250"
     CDS             443..1192
                     /locus_tag="OO566_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR6607263.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1362..>1491)
                     /locus_tag="OO566_004251"
     CDS             complement(1362..>1491)
                     /locus_tag="OO566_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCR6607264.1"
                     /translation="ERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
BASE COUNT          408 a          314 c          314 g          455 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtaaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa
      241 cttaattcta actgtttgtt ctggatgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcgcacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcctctata aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt
      481 attttcttta aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga
      541 cctttactac ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc
      601 acaaatcgga agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt
      661 gcgaaatact gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt
      721 ggcaaatgta ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt
      781 gcataaatta ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa
      841 agaatattat atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa
      901 agaacaggat acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg
      961 ggcaacagca cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg
     1021 gggttatgaa ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga
     1081 cgcagcacgt attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca
     1141 ggcacaatta caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta
     1201 gccagtacgc cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact
     1261 ggctcttttt cagggctgtg gggtatttcc agggcctctc ctttaccact ctcgggaagg
     1321 ccctttccct tcttgtcggt aatttcacta ggctgtgtcc cttaattacg taagcgttga
     1381 taaaagagtc ggatgcagcc cagtttcacc atcgccaggt aatttctcgc cgttttgtcg
     1441 taacgcgtgg cgatgcggcg atattctttc agccgcccaa agcaccgttc a
//
LOCUS       DAJTXY010000273         1463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTXY010000273 DAJTXY010000000
VERSION     DAJTXY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.278"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..775)
                     /gene="corA"
                     /locus_tag="OO566_004252"
     CDS             complement(<1..775)
                     /gene="corA"
                     /locus_tag="OO566_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6607265.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKI"
     gene            1145..>1463
                     /locus_tag="OO566_004253"
     CDS             1145..>1463
                     /locus_tag="OO566_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607266.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGDIYDESYAPLG
                     LYIE"
BASE COUNT          373 a          375 c          368 g          347 t
ORIGIN      
        1 aaattttgat gatgcggttc tgctcgatgt tgataaaacc cattgccgcc tgcatcagga
       61 agttcacctt ctggaacagg gattcgttat gcggcagcag ggattcgata tcgcgcagga
      121 tttcacgcgc ctgctccagt tgcccacccg gtaaacgcgc tttacgcacc aggaagttga
      181 gagcgcgctg ggtatccatc agacacaggc gaactttcca gccgatatct tccagttccg
      241 ccagcgtgga gagcgcctcg tcgtactcat cgccctgatg cccttccata atcacccggc
      301 tcaactgctc caggtcgcta tagatatttt caatttcatc tgccaactgt tcgattttgg
      361 tttcgaacag atccagcagc aactcgtagg cgttaccgtc taccatcgac tggctacggg
      421 cacgcatacg atacagacga aaagcgggca gttcacgctc acgcagagta aacagacgac
      481 catcacggat ggtaaatgcc acagtggagt taccggcgtg atcttccgca tcttcaaaga
      541 agaagaagga gtgaatatgc aggccgtcgt cgtcttcaaa gaaacgtgcc gatgcttcga
      601 tgtcttccag ttccgggcgg gttgccaggc tctggccaag ttcagattgt acgcgcagtc
      661 gctcgtcgtc gtccggttcg acaagatcaa tccatactgc atttacaagg ggttgtgact
      721 cttcgacttc cagccgggtc agtcggttat tttccagttg aaatgcgctc agcatgaccg
      781 ggactcccaa tgcttaaaat atcggacagt tcggtgggca cacagaaaca aattgggttt
      841 cagaccatta aacagcctga ctcagcgcga cgggaaaaat tgaggtcgct gacaaccgct
      901 aaggctatca gcaaaaaggg atagccttag gagttgatcc tggatgacag gatagtgagc
      961 cagtatctac tgggtgtgtc caaggcgaat gtcctcttag agtgatcgtg cgcgcatgtt
     1021 acgccagcaa aattttgccg tcaacacgca acagaacacc acagaacaat agttgaactt
     1081 cgcgcttttt ggtactcgtc aaggtcgcta acaggataaa aaaatcccac atccaggacc
     1141 tataatggcg tatcggctac tcattggtaa aggaatgatc accttgaatc tcaaacggat
     1201 ttttctcacc ctcaccttac ttcccttgtt tgctgttgcc gctgatgatt gcgcactctc
     1261 agatccgacg ctgaccgtac aggcgtatac cgttaatcct caaacagagc gggtgaaaat
     1321 gtactggcaa aaagccaatg gcgaagcgtg gggaacgtta catgctctgc tggcggatat
     1381 taatagtcag ggtcaggtgc agatggcgat gaacggcgac atctatgatg aaagctatgc
     1441 gccgctcggt ttgtacatcg aaa
//
LOCUS       DAJTXY010000274         1462 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTXY010000274 DAJTXY010000000
VERSION     DAJTXY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.279"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..546)
                     /locus_tag="OO566_004254"
     CDS             complement(<1..546)
                     /locus_tag="OO566_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607267.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AVTDKSYLSLNPDKYPNYRFLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..>1462)
                     /locus_tag="OO566_004255"
     CDS             complement(1208..>1462)
                     /locus_tag="OO566_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607268.1"
                     /translation="YNGADRGVFMMAKRFTALSFGCFRHDGENITVVLSRDGNTQSHD
                     LEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
BASE COUNT          424 a          313 c          318 g          407 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaaaacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgtta ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggagccgag gacaactagc taacttcttg tttcttaaag actgatgaat ccagtggtta
      841 cagtgatggt tacacatgga gaggctaaag gtcaagactt gaagtacgta acattcccac
      901 aaaaggaata gaacacagtc acggactacc cggccctttg gtctgactga gggtcgggct
      961 acggggtaaa tcgtcaggct tattgtatta gatggtctct cacatttttc agtcaaaaac
     1021 tcatcgtact ttaggccaga ccatcagcat gactatcaga gccagcttta gcaggaagac
     1081 cacgagatag actttgtgta ggtccattag gtcctcctct ttccgtaccc atgaactaga
     1141 ttactagtac aaaataaagg ccagacatcc agttacggaa gtctgaccaa taagtcgtta
     1201 ttgttagtta gcttgtctta gtaaagctca ggcgtttgac tacaggcagg acagcagcag
     1261 cgctgccagt ccccatctta acagtcgttg cagcctcagc gaccacgaag accttaccga
     1321 gaatctcaag gtcgtgagac tgggtgttgc catctcggga taagaccaca gtaatattct
     1381 cgccatcgtg acggaagcaa ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga
     1441 cgccacggtc tgcaccgttg ta
//
LOCUS       DAJTXY010000275         1366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTXY010000275 DAJTXY010000000
VERSION     DAJTXY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.280"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(1..559)
                     /locus_tag="OO566_004256"
                     /pseudo
     CDS             complement(1..559)
                     /locus_tag="OO566_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(607..1101)
                     /locus_tag="OO566_004257"
     CDS             complement(607..1101)
                     /locus_tag="OO566_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6607269.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          417 a          273 c          258 g          418 t
ORIGIN      
        1 gaattgggtt gggattctct gctatcagat ttcgcccttc ccggcgccat ttcactttcg
       61 gcgcagcatc ttcgggaata aggcggatat ttgctggtcg ccagaacagc ttgatacgcg
      121 tttttactgc tatttccagc gagttcgtcg ctttgttgct cgctgatggc ggaatatttt
      181 taatattcag ccagaagact gactcgcgat catcaggcaa tgacacacct ttggtgtgaa
      241 taatgcgtaa tattttttct tgcgaaggtt ctaatcgtga tactggcgga ataacggtaa
      301 acggcgcttt gcttttatcc tctttattaa atggatctac ccaagcctgg atgagataag
      361 gtattttttc ttctttgcta aatacagaaa tagatttatc agcattatta cttaagtaaa
      421 ccacccgcgt actaccaatg acaacgcctg cataagttaa tgctggttgg cctaaaaaaa
      481 cgctgagtat aatcgcggta aataacttac attaaactgt tgttttaaag agcctgatta
      541 aacaactttt tctggacata acgactccct ggccaaagcc aattgaataa aaaggccctt
      601 gataaattat ttcttatatt cgacagtaaa ttgtgaatca gcgttcgccg taccagccgt
      661 tacggtcgca ttggttgcca catagcgtgc aataaacttc atttcagcct tgccttcagc
      721 atcgatagcg gtataatcag agtcgttata aagtttaacc tgcgaattat cggactggtt
      781 aaaaatacga ataccaacgc cggttgccgc aatcgcttgt cctgtaccat caaaacgaac
      841 ggctgcttga gtgtacgttg ccgggcaatt ttcaagttta atactgaact tctgtggcga
      901 cgccagacta cctacacctg aaaatgcatt tttactgact ttacccagtg ggacaatttg
      961 atcggctgta tcggaggtaa cttcacaagt agtgtcgaca atttcaccga cgaagttaac
     1021 aacaccatct tctgccagtg catgcccggc cagcattaga gatgccagag tgaatgcacc
     1081 gcctataata ttacgcttca taaaaactcc attgatataa tatatccttt accatgaatc
     1141 atcacttata ttaataagtg tatttttgat tcaaaggaat agtacaccaa agagacggct
     1201 ttatctaaaa ataatattaa ttacgcaaaa ataaaatata gatttattgt ttaaatatta
     1261 tttaaacagt aaaaaatcaa gtttgttatc agacattaat gataattcag aatatattta
     1321 tatagaaaga tagatgaatt tattgatgtc agttataaaa aagggc
//
LOCUS       DAJTXY010000276         1274 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTXY010000276 DAJTXY010000000
VERSION     DAJTXY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.281"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..226)
                     /locus_tag="OO566_004258"
     CDS             complement(<1..226)
                     /locus_tag="OO566_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6607270.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEEL"
     gene            complement(226..825)
                     /locus_tag="OO566_004259"
     CDS             complement(226..825)
                     /locus_tag="OO566_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6607271.1"
                     /translation="MAHIQLVKQTSSGLLLPATSESCEFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYRTPVGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            complement(892..1152)
                     /locus_tag="OO566_004260"
     CDS             complement(892..1152)
                     /locus_tag="OO566_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607272.1"
                     /translation="MKIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHLVDGLVTDGFLLCGDEFVTSLRALKETGLKAGFSAFECE"
BASE COUNT          313 a          346 c          281 g          334 t
ORIGIN      
        1 gtaattcttc tttggtgtaa tcgctggttt ttacccgccc gtcgattaaa tcgtggcatg
       61 agctgcaggc aatcgctgcc tgcatatcgt gtggttttgt cgctgttccg cacgtccccg
      121 ccagcctgta atgcgccagc acagaagttt cgggattgtg attgcagtag ccaggaattc
      181 tgacggtgca catctgcccc cgcgccgctt tacgtaaatc caccattacg caaactccag
      241 taactgtgcg gccacatttt cgacttcctc cggagaggaa aatttacgga acaggatcca
      301 gttccacagc acattcagta cagatttata aacctgctga aactcggttt cgtccatgtt
      361 cgcaaatgcg atagattttg ccctgcgccc acgactacca tcaggataaa tatgctcggt
      421 gtaaaatccg gcctgaatgg ttacccactc gcggaaagcg tcaaacgact ttagcaatgc
      481 cgtatcccgg gttctgcatg tcgcaacggt attcagatat tgctctgcgg cttcgctcag
      541 agctggcgta tgttcccgac ctactgattc gcacaggtac tcaacgaagc ctgataccag
      601 ttctcgttct cgaggcgtga tcgccccacc gaccggagtc cggtaatcga atcccagttg
      661 caggagtttg aaaaaacgct tgtggaatgc gtagttacga acgcgcttaa aatcagcgtg
      721 tatccactcg cctgttttga tttgatgcag aaattcgcaa ctctccgacg tcgccgggag
      781 aagtaatccg gaagaggttt gtttgaccag ttgtatatgt gccatcggct ttctccggtg
      841 gcacggtgtt actcagcagg gggtcaaccc tgcactgaat tgtagatgaa ctcactcaca
      901 ttcaaaagca gaaaagccag ccttaagtcc agtctctttc agcgcccgta atgacgtgac
      961 aaattcatct ccacacaaaa gaaaaccatc cgtcacaagc ccatccacca aatgaattaa
     1021 cgcagctcca ctcttccttt gttgagactg taaacattta atacggcagt ggctgacaat
     1081 agcgccattc tcaacgcgca cagtatagag gccatcttca ctaaaaattt cacgtaattc
     1141 ttcgattttc atcaacagaa tccttccagg taaatagcac tcccctgttc gggggccatc
     1201 cctcttatcc ctgcgcgcta cttaagtgtt tttgattcta ttcctgcgcc ttctggaagt
     1261 tcaaacgagt tgaa
//
LOCUS       DAJTXY010000277         1263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTXY010000277 DAJTXY010000000
VERSION     DAJTXY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.282"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            66..1259
                     /locus_tag="OO566_004261"
     CDS             66..1259
                     /locus_tag="OO566_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR6607273.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          377 a          265 c          289 g          332 t
ORIGIN      
        1 aacgagacaa acatagcatt atgtgatcta ctgatttgtg ccaacaattc aatgagatca
       61 ctgctatgcc tgttcataaa gtatgtcaga atttcttccg ggatgcctta gcaccatttc
      121 atcagtaccg acaaaatgcc ctgatggatg ccacaatggc gttaatcaac ggagcatctc
      181 tgacgcagac cagtatcgga cgttttttgc cgggtaatgc acaagttaag aataagatca
      241 aacgtattga tcgcctcatg ggtaatgagg cgctccatcg cgatattcca atgattttca
      301 ggaatatcac ttcaatgctg acacggcagt tatctttatg tgtaatagcc gtagactgga
      361 gtggctatcc ctcccaggag catcatgttc ttcgtgccag ccttctgtgt gacgggcgtt
      421 ccattccgtt attgagtaag gttgttcctt ctgaaaaaca aaataatcca ttgatacaac
      481 atgatttcct tgattccctt gctcaatccc tgccccccga tgccagggtt attattgtca
      541 cggatgctgg atttcagagc gcatggtttc accatattac ttctctgggc tgggatttta
      601 ttggtcggat cagaaataac gttcagtatt gcctcgataa cgcgcctgaa agatggttaa
      661 aagtgtcgga tagccctgaa tgtaaaacac cggaatatat gggggctgga aggctggtta
      721 aagaaaggaa aaaaagcatc cgggggcatt tttacaccta taagaaatcg gcaaaagggc
      781 gaaaaaagaa acgttcgaaa ggtcagagtg gtttaaacaa aacagacaaa gaacagagca
      841 aatcagcaaa agaagcctgg ttgatcttca gcagtacaaa tgacttcaga gcgcgagaaa
      901 tcatcaagtt atacagtcgg cgaatgcaga tagagcaaaa ctttcgtgat gaaaagaacg
      961 ggcgcttcgg atttggtctt cgggccagca aaagtcgttc aacaggaaga attctggttc
     1021 tgagtctgct ggcaacgttg agcactatcg taatgtggtt actcggttat cacgctgaaa
     1081 ataaagggtt acacctgaaa tatcaggcta acagcattaa atcccggcgg gttatctcat
     1141 atctgacgtt agcgaagaat gttcttcgac actcgccact tattttaaga cgaacagtac
     1201 tgagcacagt tctaaaccat ctgtccagaa cctaccgaaa tatggtgctg gtttattagc
     1261 agt
//
LOCUS       DAJTXY010000278         1260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTXY010000278 DAJTXY010000000
VERSION     DAJTXY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.283"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(1..1260)
                     /locus_tag="OO566_004262"
                     /pseudo
     CDS             complement(1..1260)
                     /locus_tag="OO566_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
BASE COUNT          363 a          337 c          339 g          221 t
ORIGIN      
        1 agagaatacc cagcgcaatc agtactgcac cgctggctgc aatcatcagc atggtgtgga
       61 actgcgggtc aatctgctgg ctcaaacgac gggtcatgcc catgaagccc agcgcataca
      121 gcggcataaa ggcaacgaag aagccgatga tccaaaacca gaacgcgcgt ttaccccagg
      181 tttcgttcag tttgaaaccg aacgctttag gccaccagta ggtcatccct gcgaagcagc
      241 cgaagaccac gccgccgatg atcacgttat ggaagtgcgc aatcaggaac aggctgttat
      301 gcagaacgaa gtccgcgccc ggtactgcca gcagcacgcc agtcataccg cccaccgaga
      361 aggtgacgat aaaaccgata gtccacagca tcgcagaatg gaacacgatg cggccctgat
      421 acatggtgaa cagccagttg aagatcttca ccccggtcgg gatggcgata atcattgtgg
      481 tgataccaaa gaaggcgttt acgttcgcgc ccgcccccat cgtaaagaag tggtgcagcc
      541 aaacgatgaa cgacagcacg gtgatgcaga cggttgccca taccagcgag gtataaccaa
      601 acagacgttt acgcgagaag gttgccgcaa tttcggagaa cacaccgaaa acaggcagga
      661 tcaggatgta aacttccggg tggccccagg cccaaatcag gttgatgtac atcatcatgt
      721 tgccacccat atcgttggta aagaaatggg tgcccagata gcgatccagg gtcaacaacg
      781 cgacggtaac cgtcagaatt gggaaggaag caataatcag tacgttcgtg cacagtgatg
      841 cctaggtaaa tactggcatc ttgaacatgg tcatgcccgg tgcgcgcatc ttcagaatgg
      901 taacgaagaa gttgataccg gtaagcgtcg tacctatacc ggatagctgg agactccata
      961 tccagtaatc aacaccgact cccggactgt actctattcc cgatagcggt ggataggcca
     1021 gccagccggt ctgcgcaaat tcgcccacgc cgagagaaac gttaaccaga atcacgccaa
     1081 caacagtaaa ccagaagctt aagttgttga ggaacgggaa cgcaacgtca cgcgcgccga
     1141 tctgcagcgg aaccaccagg ttcatcagac cgataacgaa aggcatcgcc acgaagaaga
     1201 tcataatcac gccgtgcgcg gtgaagatct gatcgtagtg gtgaggtggc aggaagcccg
//
LOCUS       DAJTXY010000279         1257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTXY010000279 DAJTXY010000000
VERSION     DAJTXY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.284"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..799)
                     /gene="ipaH4.5"
                     /locus_tag="OO566_004263"
     CDS             complement(<1..799)
                     /gene="ipaH4.5"
                     /locus_tag="OO566_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6607274.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPIFTNVNISGNPLSTHVLQSL"
BASE COUNT          357 a          225 c          275 g          400 t
ORIGIN      
        1 gcagggattg cagaacgtga gtcgacaatg gattccctga aatgttaaca ttcgtaaaaa
       61 tgggcaatgt gattatattt tcgggtaatg aagttagtag gttattggaa acatccagtg
      121 ccaatagttc ttgcggtaat ctggataatc tgcgtagttg attttcacct accttcaaat
      181 attttaagct ctgaggaaat tctggtagtt cctgtagtct gttgccggaa acaaagagtt
      241 cttgtagtct atgaggcaat tcaggcaaga cagtaaggcg attaccttca atacgtaata
      301 ttgttagcgt tgatggaagc tctggtaacg tctccagact attaaaacgt gccgagagtg
      361 attgtaaata tggtggtaat gatggcagac atgacaaatt tcgattgaat gaaatattaa
      421 gcttaatcag ttgagatgga agtgtcggga gtatattcaa gttgttacca tttacatgaa
      481 gttctgtcaa aagcggagaa ttttcaggta gggagattaa ctcattgttg gaaatattaa
      541 gttcacgtat atgcaaaggg agtggtggta aagaacgtag tttcagtaag cttaaattga
      601 gaacaggctc ccgggtatcc agacatattt tcaataatcg aaccgcctgg atgcgatttt
      661 ctgttgttgt tccttcccta gcccagtcat cccagatgcg gtggtaatct ctggtacact
      721 gttcttctac cgataaacga gatatacatg ataagccaca aagggaacga aaaaaagaat
      781 gattgttgat cggtttcatt tattaacccc tcactattca tatcttagtc tattaatgca
      841 aataaaacga gaaagtcttt aaaaaaacaa tcctagagct ttatcggcgt tttcattccg
      901 gctggaggaa tatgttcctg tctttccagt aaccgtcgat tgttatatca gtctacccat
      961 gttaatgtga ccagttctac tttcatccag tttggattga tctttatccc ctatattttc
     1021 aaacaatttt tatcatttaa ctcattactg tctcgatatt cccatagcaa acgatctggt
     1081 tggttatccc ctcaaaaaag tcagttgcac gccgtaaatc cctgccggta ttgagcgagg
     1141 atttacgggt tgttgttaag gtccgtaatc gggtcacagt ttttccggag tcaatccggt
     1201 ctgcggttta tgcttatgcg acgtgattat gaatggtgca gtcgtgagcc gttttca
//
LOCUS       DAJTXY010000280         1238 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTXY010000280 DAJTXY010000000
VERSION     DAJTXY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.285"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..365
                     /locus_tag="OO566_004264"
                     /pseudo
     CDS             1..365
                     /locus_tag="OO566_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            293..766
                     /locus_tag="OO566_004265"
                     /pseudo
     CDS             293..766
                     /locus_tag="OO566_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          306 a          307 c          375 g          250 t
ORIGIN      
        1 caaggcaatg tgccgcgtac tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg
       61 gcggacaagg ataaacacac gtcagcagtt ccgtcagcac tgcgacagcg ttgtccgcaa
      121 ggcttttacc cagtccagcg ttacggtgcc ccacgcctga cggatgaact gtgtgctctg
      181 gttaccactt taacgtaaaa accgtggcag ccagtctgca ccgtcaggaa ctgagcgcaa
      241 aagcctccca gaagttcagc ccgatcagtt accgtgcaca cggcctgcct gtatcagaaa
      301 atctgctgac gcaggatttt tacgccagtg gcccgaacca gaagtgggcg ggagacatca
      361 cgtactatta cagctcaccg actgcgggga aacacggggc gccgggttat tgaaggtgac
      421 ctgtccagct acttcgacac agtacatcac cggctgctga tgaaagccgt ctgccgcagg
      481 atcagtgatg cacgttttat gagactgctg tggaaaacgc cgtgttgatg ttggtctctt
      541 ccgggcggtc agtgaaagta tgccacaggg cggtgtgatc tcgccgttgc tgtcgaacat
      601 catactgaat gagttcgacc agtatctgaa taaacgctac ctgagtggaa aagccagaaa
      661 ggatcgctgg tactggaatc acagtatcca gcgaggccga agtactgctg tcaaagaaaa
      721 ctggcaatgg aaacctgccg ttgcttactg ttgctatgcc gatgactgtg tgccacgaag
      781 gcgcgtcctg gggacgtgag tccctgtgca gccatgcagc acaagagatg agggaaggcc
      841 cctcggtgtc ggcgtgcagg cgcaggcacc aaaccatcca ctgctgctgc atttaagaga
      901 tgtggtggtg agcgagatgg gaaaaggtcg gacgtgagcc gatcaggtat ggttcgaaga
      961 gacgaacgaa agtgaaccgc tgatgatgtg tcgaaagcat atagatgatg tcaaaacaat
     1021 ggtggagtcg ttcctgtggg atatgtttac tgcctgcgcg gcatccggca taaaagcggc
     1081 gtgaagtcga ttcaggctct tgtggggaac gtggacctgt caccccgatg tgaagggaga
     1141 agcgcaagtg gaagccccat gagcgccaga gtaccaatgc ggggtacagg gacggagcac
     1201 caagcgattg cgcagtaaac atgcttgaca acttaatt
//
LOCUS       DAJTXY010000281         1214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTXY010000281 DAJTXY010000000
VERSION     DAJTXY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.286"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..141)
                     /gene="hokD"
                     /locus_tag="OO566_004266"
     CDS             complement(<1..141)
                     /gene="hokD"
                     /locus_tag="OO566_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6607275.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAY"
     gene            complement(793..1209)
                     /locus_tag="OO566_004267"
     CDS             complement(793..1209)
                     /locus_tag="OO566_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607276.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
BASE COUNT          314 a          305 c          224 g          371 t
ORIGIN      
        1 gtaagctgtg aagacagcga cctccgtctg gccggttcgg attcgtacct cgcagaggtc
       61 tttcctcgtt accagtgccg tcactatgac ggttaaacag atgacgataa gggcgattaa
      121 catcgccttt tgctgcttca tagcctgctt ctccttgcct ttcggcacgt aagaggctaa
      181 cctacatgtg ttcagcatgg attgagcctc agattaatgt taagcgtctt gcaggacgcg
      241 taatgttaac tggggctttt ctctatctgc cttttggtgt tcatgcctga gacagatagc
      301 ctcaagcacc cgcagtcatt ctacttaact aagatttccc tgcaaaccgt ttttgtccgg
      361 cacagtaaat atccaactaa accaatagcg ttcgctgtat ttaccgccag tattcaatgc
      421 acatgaccgc catgaacacc cctaaaaaaa gggcatttat atgtccaaac attaatatca
      481 aaacatcaat tttttccata taccttgctg tgaagatgat gggcatacat gatgcgaaca
      541 accagaacgc aacaaacaaa aactgcaatg cgtttttcat tattccccct acaatcaatg
      601 tgcaataaca tttaaacaca cctcaatttg gccggacata taaatatcta aaccagaaaa
      661 aatcacttac atagcgttac aaactcttta gtctaaatac tcatcgtaaa acattcccca
      721 tacttatcag tccgttccgc gccaggtagc ttattgcctt atctggcaac ctataatcag
      781 gtttccgctt tttcagttgg ctggtcgttt aaccgacata gttaacccat taatctggtt
      841 gccggatgct ggtggatttt cgcgttttag ttgttcataa aagtgcacag ctttaaccag
      901 ttcttctgat gtaaccggga ctggtggggc agtgaataag gcctgaattt catagttcgg
      961 cctgtcgtta caatcctctt ttttcggtac atatttccag tcaccaaccc acaactcccc
     1021 ctgagagtcc ataacacctt ttttcacgta gcgatatcgc cacgctatcg gctctgcttc
     1081 cagcgatgcc cgtgcaattt tgaataactc gccctctact cgcgccatcc ctgaattggg
     1141 gtggcatttc gtaatcgcta tttttaattt ggcttcttcg attaattgtt cttttgttaa
     1201 ttcagtcatt ttca
//
LOCUS       DAJTXY010000282         1213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTXY010000282 DAJTXY010000000
VERSION     DAJTXY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.287"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            55..588
                     /locus_tag="OO566_004268"
     CDS             55..588
                     /locus_tag="OO566_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6607277.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(617..1144)
                     /locus_tag="OO566_004269"
     CDS             complement(617..1144)
                     /locus_tag="OO566_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6607278.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1146..>1213)
                     /locus_tag="OO566_004270"
     CDS             complement(1146..>1213)
                     /locus_tag="OO566_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6607279.1"
                     /translation="GVYYRPIQKYINGTWYNVASV"
BASE COUNT          342 a          274 c          264 g          333 t
ORIGIN      
        1 gccaattcag tattgcatta atgggacgtg gtataacgcg gggagtattt aacgatgatg
       61 cacttaaaaa acatttctgc tggtaatcct gaaacaaaag agcaatacca gctaacgaaa
      121 caatttaaca tcaaatggct ttatacagag gatgggaaaa actggtatga ggaacaaaag
      181 aactttcagc ctgatacgtt gaaaatggtc tatgaccaca acggcgttat tatttgtatt
      241 gaaaaggatg tttcagcaat taatccggaa ggcgcaagcg tcgttgaggt tcctgatatt
      301 acagcaaatc gccgggctga tatttcaggt aaatggatgt tcaaagatgg catagtgata
      361 aagcgaactt ataccgggga agagcagagg caacaagtgg aaaatgaaaa gcaaagcctg
      421 ttgcaacttg tcagggataa aacccagcta tgggactcac agctacgact gggcatcatt
      481 tccgccggga ataagcagaa attaaccgag tggatgctct acgcgcagaa ggtcgaatcc
      541 acagacacct ccagtctgcc agtaacgttt cccgaacaac cagaatgaga gaaggcccgc
      601 tatcgggcct taatttttac tctggttttt gtggccattc tggctttgcc gtatccacac
      661 ggctgaccag aacactgtag cgttcccatg actccagtcg tgcgcattcc tcatctgttg
      721 ccatattcag cctgacagag cgctccagcg gctggatgac tgattcagct tcggaaagca
      781 atgcggcttt ttgtgattcg gcctgttgct gctgttcgtc tgccgtataa atccgcttaa
      841 ccacagctcc gtccttaaac atccacttcc cggaatcatc ggcacgacga tttgctgtta
      901 tatcggggac ctcaacgacg ctaaagcctt caggattaag cgtggaggca tctttagtga
      961 tggcgacaat aatattattt tcatcgtaaa caatctttat tgtatctggc tgaaagttct
     1021 tcacttcccc ataccagttt tttccgtctt ctgtccacaa ccagattacg tcaaaattct
     1081 ttgttaattc gtactgttcc agtgttttag cgttacccgc tttaatgttc tttaagtgca
     1141 tcatattaaa cgctcgctac attataccaa gtgccattta tatacttttg aatcggtctg
     1201 taataaacac ccg
//
LOCUS       DAJTXY010000283         1198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTXY010000283 DAJTXY010000000
VERSION     DAJTXY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.288"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            17..928
                     /locus_tag="OO566_004271"
     CDS             17..928
                     /locus_tag="OO566_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6607280.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            944..1171
                     /locus_tag="OO566_004272"
     CDS             944..1171
                     /locus_tag="OO566_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607281.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          334 a          255 c          335 g          274 t
ORIGIN      
        1 cgacactatt aacgctttga ttaacgaggg cgacaagtct caggaagata ttgctgatga
       61 agccggaatg tctccggcaa cactctcggc tctgcgtaaa ggagagtaca acggtaatgt
      121 cgaaggtatg catgacaaat tacagaaatg gtatcaggtc tggcagaagg gacagaactt
      181 gtcaggtacc cctggtgttg tgaagaccca gatattttgt gatttgcatg agctgtttaa
      241 aacagtgcgc tcactcggga ttatcagtgt cgttgttggc gtaccgggtg tcggaaaaac
      301 ctttgctgcc cgggaatatt gtcgcaggac agctaatgcc tggatggtga ctcttgctcc
      361 ggcacattcg agcgtaacgg aatgcctcct tgaaatagca tatgcgctgg aaattaataa
      421 tccgggaaga aataagggga atataacccg cgccattcgc agaaaactgg gtggtggtaa
      481 agaaaccagt ggccgtgtta acccccttct gattattgat gaagctgatc acttaagtgt
      541 ggatggtctg gagcaattac gcgccattca ggatgccact ggcgtgggga tggttttaat
      601 tggcaacccg aaacagatgg cggatgccac ccgtcgtggg actgatgaac tggcccgtct
      661 gttcagccgt tttgccatga caaaacaact gcgcaaagta aaaaagaccg atgtgatggc
      721 tgtagccaaa gcatggggag taacacagga agatgagctg gacctgctga tgagggtagc
      781 cgaaaagccc gggggattgc gtgtattaac tcacacactg aatcatgcgt ggctggcggc
      841 aaaaggtgca ggcacatccc tgaataaaag tcacattaaa gcggctttta aggaggctta
      901 cagcaacccc aggttactga actggtagta aaggacataa accatgatgt cacgaaatat
      961 aaaaatggca acggatgcgc agaactggtt acaggcgcac gggagtcacg taaatgaatc
     1021 atatctcggc gtggcgcgtc cggtacttga aatcacctgc ccaccaccgg agctggtcag
     1081 aaacgctgtc aggattatgg agcataaatc aggggttgcc cgttcagtat ggacggcccg
     1141 ccttaatggc tgccagatta tctggcgtta ataacggctt atttatttgt gaggaata
//
LOCUS       DAJTXY010000284         1159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTXY010000284 DAJTXY010000000
VERSION     DAJTXY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.289"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(133..810)
                     /locus_tag="OO566_004273"
     CDS             complement(133..810)
                     /locus_tag="OO566_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607282.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
     gene            complement(946..>1159)
                     /locus_tag="OO566_004274"
     CDS             complement(946..>1159)
                     /locus_tag="OO566_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HCR6607283.1"
                     /translation="NSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRS
                     ETVLQKSLEITEEADLALRNAMARIK"
BASE COUNT          288 a          283 c          257 g          331 t
ORIGIN      
        1 cgcctctgta gtattaacgt atttgatgtt gtttggcagt tttacttgtt tcgccgtagc
       61 gaagacagag aacagaatag ttacagcaac gagtaaagtt ttgataaaag ttttcatgac
      121 agactcctta aattagttaa ccgcagctac gtagtttttc accagatacg cgaattcatg
      181 cacgccaatt tcgccgaacc aaaccaggta ggccgggtca tctaaaccaa agtagcggta
      241 gccaacgact tcatgggttt ttgggttata gagtgttacg ataaagtccc agccgcaggt
      301 attggctttg caaccgtcgc cctggagcca gactgtaccg ttaatggtta ctggcgttac
      361 gtttccgcca gggccatcca tatacccgcc tttaccgaac atgccgctca gtccgaccgg
      421 ttttaatgct ttgtcaagcg cagctttgta ggcagcgtca ccttcgtaca actcgtacgt
      481 tgttggacac gctccaggtt gttcagcccg ggcgtttggg tcgcagctga gcgcttgttt
      541 cacgctcgct gcttccacgt taaagccagt aaacagaacc gccagaaata acatcagggt
      601 atgtaaacgt ttcattgtaa atattcttcc gttgtaaatg gcgctggaaa atttctcatc
      661 agtgaaacct tccggaaatt tccccccctt gtatgatgta tatcgccagt attttatttc
      721 atcccggata taatttttat atttcccggc acgtgcaata acgctgacca gagatataag
      781 gctcattaat aactttacag gtaattgcat aattaaattt ccagtgagtg aattgacagg
      841 tgaggggtaa aactttctct caggtaatat atatctgaaa atattttcaa taacaccagc
      901 taggaaaaac accaccacag gcggtggcgt ttctttggag cgtctttatt taatccgggc
      961 catcgcgttt ctcagtgcca aatctgcctc ttccgttatc tccagacttt tctgcaacac
     1021 cgtttctgac cgggacagta cctgacgtgc tgccagcggt ttccggatat gctccatcgt
     1081 cagcatcgtg tcttcatagc cctgctgacg ccaggcgtcg gtatgtgcca acgtgaaact
     1141 gagcagaccg ccgctgttg
//
LOCUS       DAJTXY010000285         1147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTXY010000285 DAJTXY010000000
VERSION     DAJTXY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.290"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(52..177)
                     /locus_tag="OO566_004275"
                     /pseudo
     CDS             complement(52..177)
                     /locus_tag="OO566_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(249..1133)
                     /locus_tag="OO566_004276"
     CDS             complement(249..1133)
                     /locus_tag="OO566_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCR6607284.1"
                     /translation="MVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDD
                     PFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETT
                     DLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLL
                     RLCLRQALQLADVVKISEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVC
                     YRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGAL
                     AVTAKGAMTALPCRQELE"
BASE COUNT          303 a          332 c          241 g          271 t
ORIGIN      
        1 aggtgagcat atcacctctg ttcaggtggc caaattcagt gtgccacttc accctagatg
       61 tcctttcagc caaatagcgt ataacgacca ccacagcgac caggaaataa aaaagagaaa
      121 tgagtaactg gatgcaaaac gatagtacgc atttctgaat ggaatattca gtgccataat
      181 tacctgcctg tcgttaaaaa attcacgtcc tatttagaga taagagcgac ttcgccgttt
      241 acttctcact attccagttc ttgtcgacat ggcagcgctg tcattgcccc tttcgccgtt
      301 actgcaagcg ctccgcaacg ttgagcgaga tcgataattc gtcgcatttc tctctcatct
      361 gtagataatc ccgtagagga cagacctgtg agtaacccgg caacgaacgc atctcccgcc
      421 cccgtgctat cgacacaatt cacagacatt ccagcaaaat ggtgaacttg tcctcgataa
      481 cagaccacca ccccttctgc acctttagtc accaacagca tggcgatctc atactctttt
      541 gccagggcgc atatatcctg atcgttctgt gtttttccac tgataagtcg ccattcttct
      601 tccgagatct tgacgacatc cgccagttgt agcgcctgcc gcaaacacaa gcggagcaaa
      661 tgctcgtctt gccatagatc ttcacgaata ttaggatcga agctgacaaa acctccggca
      721 tgccggatcg ccgtcatcgc agtaaatgcg ctggtacgcg aaggctcggc agacaacgca
      781 attgaacaga gatgtaacca ttcgccatgt cgccagcagg gcaagtctgt cgtctctaaa
      841 aaaagatcgg cactggggcg gaccataaac gtaaatgaac gttccccttg atcgttcaga
      901 tcgacaagca ccgtggatgt ccggtgccat tcatcttgct tcagatacgt gatatcgact
      961 ccctcagtta gcagcgttct ttgcattaac gccccaaaag gatcatcacc gacccgacct
     1021 ataaacccac ttattccgcc taatctggcg attcccaccg caacgttagc tggcgcgccg
     1081 ccaggacaag gcagtaggcg cccgtctgat tctggcaaga gatctacgac cacatcccct
     1141 aaaaccc
//
LOCUS       DAJTXY010000286         1146 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTXY010000286 DAJTXY010000000
VERSION     DAJTXY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.291"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(22..174)
                     /locus_tag="OO566_004277"
     CDS             complement(22..174)
                     /locus_tag="OO566_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607285.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            192..383
                     /locus_tag="OO566_004278"
     CDS             192..383
                     /locus_tag="OO566_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6607286.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            694..>1146
                     /locus_tag="OO566_004279"
     CDS             694..>1146
                     /locus_tag="OO566_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6607287.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAV"
BASE COUNT          322 a          247 c          222 g          355 t
ORIGIN      
        1 tgaattatta cgtttatcat gttaattcat cattattaca tcatcattgt aaataattaa
       61 attaacttcc ataacattaa aatatgtatc cactgacgct tttttacata acgaagaatt
      121 gaccattttg tcctgttgtg ccttaatgta agtaccgtcc acagcgtggg acatacttca
      181 aggaaccttt tgtgagtcag gcaactagta tgcgaaaacg acaccgattt aacagtcgca
      241 tgacccgtat cgtactgctc atcagcttta tcttcttctt tggccgtttt atctactcgt
      301 ccgtcggtgc ctggctgcac catcagagca aaaaagaagc tcagcaatcc acactctccg
      361 tcgaatcacc ggtacaacgt tagcggttac cttctccact ttcacagaac ataacggcac
      421 ttcgctgtcg gatgcttttg ctctttggga ttatcaaagc ggcagatatt ctttcatctt
      481 aaattttacg tctttgtcct gactgatgtt tatcctgttt ggctgcgaaa taaatataaa
      541 attaatatat atgttgtaat gatatatttt tataaattat tccctgcgtg aattttaata
      601 aatttaatct atccctttat acgcaataca tttactttcc tcttttgatg atcttaaatg
      661 tcttattttt cgtaatgtgt ataacaagga atagtgatga aatttaaaaa atgtcttctg
      721 cctgtggcaa tgttagcgtc attcactctg gcaggatgcc agtcaaatgc tgacgatcat
      781 gccgccgatg tttatcaaac cgatcaactg aataccaaac aagaaactaa aaccgttaat
      841 attatttcca ttcttcccgc aaaagttgcc gtagacaact cccaaaataa acggaacgca
      901 caagccttcg gcgcgcttat tggcgcagtc gctggcggtg ttatcggcca caacgtcggt
      961 tctggcagca attccggaac gacggcaggg gcagttggcg gcggagctgt aggcgcggca
     1021 gcgggttcta tggtgaatga taaaacctta gtggaaggtg tttctttaac atataaggaa
     1081 ggcaccaaag tgtatacctc cacccaggag ggtaaagagt gccagtttac gacaggttta
     1141 gccgtt
//
LOCUS       DAJTXY010000287         1134 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTXY010000287 DAJTXY010000000
VERSION     DAJTXY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.292"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..452
                     /locus_tag="OO566_004280"
     CDS             <1..452
                     /locus_tag="OO566_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6607288.1"
                     /translation="NRKKELTEPQIREGSWRKERRTSYSREFKVRLAKQALQPGAVVA
                     RIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNK
                     HDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            449..796
                     /gene="tnpB"
                     /locus_tag="OO566_004281"
     CDS             449..796
                     /gene="tnpB"
                     /locus_tag="OO566_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6607289.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            816..>1134
                     /locus_tag="OO566_004282"
     CDS             816..>1134
                     /locus_tag="OO566_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6607290.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          314 a          285 c          315 g          220 t
ORIGIN      
        1 ccaacagaaa gaaggaatta acagagcctc aaatcagaga aggctcatgg cgaaaagaac
       61 ggcgaaccag ctatagccgt gaatttaagg tccgtctggc taagcaggcg ttacagcctg
      121 gggctgttgt tgcccggatc gccagagaac acgatatcaa taataacctg ctgtttaaat
      181 ggaaaagcca gtacgaggac ggcttactga gcgatgatga catacaggaa tgcatgcctg
      241 tcccggtggc actgactgat acgccggagc cgaccagacc agttacaaat cccttctggc
      301 gtaacaagca tgatgagcgc cctgaggggg ctcccggaaa cgtcccacgg tgcgagctgc
      361 atcttaaatc aggtgtggta aaactgtttg accctctcac tccggaactg ttacgggcgc
      421 taatccgcga aatgaaaggg ggtatccgat gataacgctg ccgaccggta ccaaaatctg
      481 gatcatcgct ggcatcacag atatgcgttg tggcttcaat ggcctggctt cgaaggtgca
      541 gaacacgctg aaagatgacc cgttctccgg gcatatcttc gtcttccggg gccgcagtgg
      601 caaaatggtg aaaatactgt gggccgatcg tgacgggtta tgcctgttcg ccaaacgcct
      661 ggaacggggc cgcttcgtct ggccggtgac ccgggaaggg aaagtgcacc tgacgccagc
      721 tcagttatcc atgctactgg aggggatcgc gtggcaacat cccaaacgga cagaacggcc
      781 tggcatccgg atataacccg tgataaaaca agggaatgaa caatgaactc cccgatgata
      841 ttgagctgct taaagccatg ttgcgtaagc aacagagtcg gcttcgacag tatgcctgtc
      901 aggtcgcggg ctatgagcag gaaattgaac ggctgaaagc gcaactcgac aggttgcgtc
      961 gtatgttgtt cggccagagt tcagagaaaa agcgtcataa gcttgaaaat cagatccgac
     1021 aggcagaaaa acgactgtcg gaactggaaa accggctgaa cacagccaga aatcttctgg
     1081 aagatgcatc gtcagtcaca gattcacctg acaccagtcc cccgtcagaa aacc
//
LOCUS       DAJTXY010000288         1085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTXY010000288 DAJTXY010000000
VERSION     DAJTXY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.293"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..476
                     /gene="ybcL"
                     /locus_tag="OO566_004283"
     CDS             <1..476
                     /gene="ybcL"
                     /locus_tag="OO566_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6607291.1"
                     /translation="NEIKTGEQLTTSHVFSGFGCEGGNTSPSLTWSGAPEGTKSFAVT
                     VYDPDAPTGSGWWHWTVANIPATVTYLPTDAGRRDGTKLPTGAVQGRNDFGYAGFGGA
                     CPPKGDKPHHYQFKVWALKTDKIPVDSNSSGALVGYMLNANKIATAEITPVYEIK"
     gene            486..>1085
                     /locus_tag="OO566_004284"
     CDS             486..>1085
                     /locus_tag="OO566_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6607292.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
BASE COUNT          341 a          203 c          222 g          319 t
ORIGIN      
        1 gtaatgaaat aaaaacagga gagcaactta caacgtctca tgtattttct ggatttgggt
       61 gtgaaggtgg taatacatcg ccctcattaa cctggtctgg tgctcctgaa ggcaccaaaa
      121 gctttgccgt aactgtgtat gatccagatg ctcctacagg cagtggatgg tggcactgga
      181 ctgttgctaa tattccagca actgtaacat atttgcccac tgatgcagga agacgtgatg
      241 gtacaaaact tccgactggt gctgttcaag gccgaaatga ttttggttat gcaggatttg
      301 gtggagcatg tcctcccaaa ggagataaac cccatcatta ccagtttaaa gtatgggctc
      361 taaaaactga taagattcct gtggattcta attccagcgg agcgttagtt ggttatatgc
      421 ttaatgccaa taaaattgca accgctgaga taacaccagt ttatgagata aagtagggta
      481 agagtatgct ggcaaaagat aagtctaacc taaagattga agaaatacgg atgcataaac
      541 atcatgagat tcacagggtt aagcctctta tgccagcttt atgtcgtatc cgtcagggaa
      601 aaaaagttat caattgggag acgcatagtc taactgttga taataatcag ataatattat
      661 ttccctgtgg atatgaattt tatattgtga attatccaga agcaggtctt tatcttgcag
      721 aaatgcttta ttatcccatt gatctaattg aaaagtttca aaaattttat gcgataactg
      781 atcaaattcg taacacgaca ggtttctgct tacctcagaa ccccgagtta atatattgtt
      841 gggagcaact aaaaacatct atttcccgag gcttctcaac gcaaattcag gaacacttag
      901 caatgggcgt tctactttca ttaggagcac atcatgttaa ttgtttactt ttatcagata
      961 gtaaacaatc attaataagt cgttgttata acctaatgct atccgaacct agaacaaaat
     1021 ggacagcaaa caaggtagcg agatatctct acatttctgt ttccacattg catcgccgtc
     1081 tggca
//
LOCUS       DAJTXY010000289         1058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTXY010000289 DAJTXY010000000
VERSION     DAJTXY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.294"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..444
                     /locus_tag="OO566_004285"
     CDS             <1..444
                     /locus_tag="OO566_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6607293.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPES
                     PAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVI
                     PLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            445..810
                     /locus_tag="OO566_004286"
     CDS             445..810
                     /locus_tag="OO566_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6607294.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            807..>1058
                     /locus_tag="OO566_004287"
     CDS             807..>1058
                     /locus_tag="OO566_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6607295.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVE"
BASE COUNT          293 a          246 c          294 g          225 t
ORIGIN      
        1 ccgggagagc agaccgccac cagcatactg aagcagcgca caaaaaatct tgcgccgctg
       61 cctcacgccc accagcaaca gaatccacca caggaagaga cggtggtcag cattgccgtt
      121 gatccggaat ctccggctca gtatctccag cgccagaaac cacaacggga agagatgcct
      181 gtatacacgc gctgggtaaa aacgcagaaa tgcatgacgt gtggcaatca ggcagatgat
      241 ccgcaccaca tcattggtca tggactggga gggatgggaa caaaggctga cgatttgttt
      301 gttattccgc tgtgtcgtaa atgccataac gaactacacg ccggggtaaa agattttgaa
      361 gaaaaacacg gtagtcagtt gttgttgctg attcgttttt taatgcacgc gagaaattcg
      421 ggtgttctga agtggaaagc ataaatgacc gaacgcatag aatttgtttt gccttacccg
      481 ccaacggtga acacctactg gcgacgtcgt ggcagcacat attttgtatc aaaagccggt
      541 gagcgttatc gccgtgatgt ggcgctaatt gttcgccagc agcgactgaa attaaacctg
      601 tccggaaggc tggcgataaa gattattgca gagccaccgg ataagcgccg tcgtgacctg
      661 gacaatatcc tgaaggcacc gctggatgcg ctgacgcatg ctggactact tatagacgac
      721 gagcagtttg atgaaatcaa tattgtgcgc ggtcagctcg ttcctggtgg gcggctgggg
      781 ataaaaatca cagaactggg gtgcgcatga ataaccagta tttacagttt gtgcgtgagc
      841 agctcattat cgccaccgct gatttgagtg gggcaacaaa aggtcagctt gaagcctggc
      901 aagagaatgc catgttcgat acagggcgtt acaggcgtaa aaaaatccgg taccgcgatg
      961 aagtgactgg aaaaatgata acgcgggata atccaccaat cccgggaaag caatcgctgg
     1021 cgaaggggac gtcaattcct ctggtcagtc cggttgag
//
LOCUS       DAJTXY010000290         1048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTXY010000290 DAJTXY010000000
VERSION     DAJTXY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.295"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..463
                     /locus_tag="OO566_004288"
     CDS             <1..463
                     /locus_tag="OO566_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR6607296.1"
                     /translation="RVSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRV
                     DLVQRAQLHLEQIFSWWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQL
                     LDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGD"
     gene            485..604
                     /locus_tag="OO566_004289"
     CDS             485..604
                     /locus_tag="OO566_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607297.1"
                     /translation="MIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            601..>1048
                     /locus_tag="OO566_004290"
     CDS             601..>1048
                     /locus_tag="OO566_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR6607298.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIATSPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALA"
BASE COUNT          236 a          251 c          314 g          247 t
ORIGIN      
        1 cagggtcagt tcactggtca gtgatactgc caggctggcg cattattccg cagatacgcg
       61 ggagcagtta ctcaccctgg ccgatcaggt tcaccagaaa ctgaatcatc ttgaagaaaa
      121 actccaccgt gttgacctgg ttcagcgggc gcagttacat cttgagcaga tattctcctg
      181 gtggagcgcc gggcgatatg cgtcattttc ccctgccgga cgttgttatg tggcacttga
      241 agagcttcgc tggggcgcgt ttggtgatgt gatacgtcag ggcgaaacag gccaggttaa
      301 ccagctactg gatattctca gacataaagc attaacgcag atggcacagg agagtggcgg
      361 tagtgcaacc gtgcgtctga acactctgga ctggcttggt ggtcagggcc gggagcaggc
      421 agataacgag tggcatgacg cgattaactg gctgggagac tagtgcagtg aagagcagca
      481 tccggtgatc tggtcaacca cgcaggctgc agaacactta ccggttcgca tgcctcgcct
      541 ctgctctgca gaacgcctct ctgaaagtat ggtcgatgaa atatttcaga agggggctgc
      601 atgagtacag aaatgaaaac ggggctggtg ctgtccggtg gcggggcggt gggcgcttat
      661 caggcgggag tggttaaggc actggcagag tgtgatacac agatcagcat ggtttcaggg
      721 gccagcattg gcgcattcaa tggtgccatt atcgcgacct ctcccgatct gtcagaagct
      781 gccgtacgcc tggaagcgct ctgggatcat ctggggaata atcaggtgct gtcggtaaac
      841 cgattggttt acttttcatt gctgaaaaaa ttgttccagg caatgaacct ctgccagatc
      901 cccggacgtg caggagcact gcttacgacg cttcttcgcc atatatcgat actcaacggg
      961 tttgacaatc tgatggctca gccgttgttg tcagatgagc ccctgacagc gctgttggat
     1021 cattatcttg atactgatgc tctggcag
//
LOCUS       DAJTXY010000291         1041 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTXY010000291 DAJTXY010000000
VERSION     DAJTXY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.297"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            11..1041
                     /locus_tag="OO566_004291"
                     /pseudo
     CDS             11..1041
                     /locus_tag="OO566_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          261 a          275 c          293 g          212 t
ORIGIN      
        1 ggtgactcac atgacaaaaa cagtatcaac cagtaaaaaa ccccgtaaac agcattcgcc
       61 tgaatttcgc agtgaagccc tgaagcttgc tgaacgcatc ggtgttactg ccgcagcccg
      121 tgaactcagc ctgtatgaat cacagctcta caactggcgc agtaaacagc aaaatcagca
      181 gacgtcttct gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct
      241 ggcagaacgg gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg
      301 cctgaaatga agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg
      361 tgccgcgtgc tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg
      421 ataagcacgc gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc
      481 cggtcaaaac agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac
      541 ccctttaacg taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc
      601 tcccggaagt tcagcccggt cagctaccgc gcacacggcc tgcctgtgtc agaaaatctg
      661 ttggagcagg atttttacgc cagtggcccg aaccagaagt ggccaggaga catcacgtac
      721 ttatgtacag atgaaggctg gctgtatctg gcagtggtca ttgacctgtg gtcacgtgcc
      781 gttattggct ggtcaatgtc gccacgcatg acggcgcaac tggcctgcga tgccctgcag
      841 atggcgctgt ggcggcgtaa gaggccccgg aacgttatcg ttcacacgga ccgtggaggc
      901 cagtactgtt cagcagatta tcaggcgcaa ctgaaacggc ataatctgcg tggaagtatg
      961 agcgcaaaag gttgctgcta cgataatgcc tgcgtggaaa gcttctttca ttcgctgaaa
     1021 gtggaatgta tccatggaga a
//
LOCUS       DAJTXY010000292         1040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTXY010000292 DAJTXY010000000
VERSION     DAJTXY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.298"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>1040
                     /locus_tag="OO566_004292"
     rRNA            <1..>1040
                     /locus_tag="OO566_004292"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          258 a          242 c          329 g          211 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaaccttacc tggtcttgac atccacggaa gttttcagag atgagaatgt gccttcggga
      541 accgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt
      601 cccgcaacga gcgcaaccct tatcctttgt tgccagcggt ccggccggga actcaaagga
      661 gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg
      721 accagggcta cacacgtgct acaatggcgc atacaaagag aagcgacctc gcgagagcaa
      781 gcggacctca taaagtgcgt cgtagtccgg attggagtct gcaactcgac tccatgaagt
      841 cggaatcgct agtaatcgtg gatcagaatg ccacggtgaa tacgttcccg ggccttgtac
      901 acaccgcccg tcacaccatg ggagtgggtt gcaaaagaag taggtagctt aaccttcggg
      961 agggcgctta ccactttgtg attcatgact ggggtgaagt cgtaacaagg taaccgtagg
     1021 ggaacctgcg gttggatcac
//
LOCUS       DAJTXY010000293         1016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTXY010000293 DAJTXY010000000
VERSION     DAJTXY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.299"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..674)
                     /locus_tag="OO566_004293"
     CDS             complement(<1..674)
                     /locus_tag="OO566_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6607299.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFL"
     gene            complement(674..>1016)
                     /locus_tag="OO566_004294"
     CDS             complement(674..>1016)
                     /locus_tag="OO566_004294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607300.1"
                     /translation="KRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECC
                     ADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMYCPECGT
                     VHWPDGVAPPF"
BASE COUNT          235 a          297 c          256 g          228 t
ORIGIN      
        1 tccaggaagc gattccattc ccgtttactg cttccttctg ctgccagcgc atccggcatc
       61 accaggtcag ggtatttccg tgacagcaac cgtgttatcc ggtagcgcca catgctcatc
      121 accttacggg cgtaaaaatg aagatttttc caggtgtggc ccgacgtcac accaccggca
      181 gttgtcgata aatggatatg cggatgccac tgctggtcac gcccccatgt gtggatcacc
      241 gtgaatatcc ccggctccac atctgcctga tggcagattt ccagtatcac atccgctgca
      301 atgcggctca tctctgccag taaccaccgg ttgtggaaca ccagggacca gtactggcag
      361 ggaagtgtga acacaatatg ctgccacggg cagtcgggga ccaggctcag cagatactgt
      421 atccactgtg cgccagcctt caccccgcag tgcgggcagg agcggctttt acaccggaag
      481 cagacctttt ttgtatggca acagtccggt gatgaacaac accactgcgt gtagcccatc
      541 agtgtggtcc cgcaggccat gatttttgtc actgattcaa ttaccaccgg acggactgcc
      601 ccttccggtt gtttctccag ccagttaagc cagcggtttc cctgctgaaa aatatcggca
      661 aaacggggaa gcatcagaag ggcggggcga ctccgtccgg ccagtgaacc gtgccacact
      721 ccgggcagta cataccgccg gcgctgatac cggcaagaat ggtcgcaaat tcccgctccg
      781 tgcagcgggc gatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagct
      841 ttttgtttcc cgcatccaca aagaacggaa tattcaggtc tgcgcagcat tcaacggcat
      901 cgtcaaaact atcaaagcgc agaacttctg cgtcttcttc gtcaaaaaaa tcatcttcgt
      961 gaagcttcac gacatagcgg ggaagtttgc ttctttgaga ggcgggttta cgttta
//
LOCUS       DAJTXY010000294         1003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTXY010000294 DAJTXY010000000
VERSION     DAJTXY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.300"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..282)
                     /locus_tag="OO566_004295"
     CDS             complement(<1..282)
                     /locus_tag="OO566_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR6607301.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETL"
     gene            379..>1003
                     /locus_tag="OO566_004296"
     CDS             379..>1003
                     /locus_tag="OO566_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6607302.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDL"
BASE COUNT          296 a          201 c          250 g          256 t
ORIGIN      
        1 cagcgtttca ctttccgctt ctgaaaccag tacggttttt aaaagtacgt tgcctgtcgc
       61 aacaaactta tgcggcattt gtggcggcag ccaaagcgcg ctgctggcat ggactagcca
      121 gacgctttct ccagcccata cacgaacgac tccttcagcc gggtaaagca actgggcata
      181 gagatgttta tgccagggaa ctgtacctcc attcagatgt tccgaatcca gtccgataat
      241 gtgatgccct ggtgatgctt tgggttgcag tgtaattgtc atttacagta ggtattttgt
      301 cagaaatcat caaataagcc atgttaaatt ttaaatatga cgtatgcaag tttccggata
      361 tatacggtgt aaagagtgat gataatttct aaaaaacagc ttattggggt ggtagcgatc
      421 ggtattcttt tgactggcgt tgtctttttt atctggaggg taagcaaagg aagatttata
      481 cagacgacgg atgatgctta cattggtggt aatatcacca cggtggcaag taaagtttct
      541 ggttatattt ctgctataga ggtgcgcgat aatcagagcg taaaaaaagg cgatattatc
      601 ctgcgtctcg atgatcggga ttatcgtgca aatgtcgcca ggcttgaggc aaaaataaaa
      661 agtagcaaag ccaatctcga aggcattcaa gccactatta cgatgcagca gtcgataatt
      721 caaagtgcca gtgaaacatg gcaggcagta aaacatgagg agcaaaagcg cctgcgagat
      781 actgaacgtt atgaaaaact cgcacaatca gcggcaatat cgcaacaaat tattgataac
      841 gccaggtttg actaccagaa ggtcgcagca aaggagcgca aagccgcgaa cgatttcctg
      901 gtggagaagc aacggctagc agttttatct gctcaggaag aaaacgtacg tgcgagtatc
      961 gaagaagttc aggccgcttt gactcaggca ctactggatc tgg
//
LOCUS       DAJTXY010000295          983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTXY010000295 DAJTXY010000000
VERSION     DAJTXY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.301"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO566_004297"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO566_004297"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6607303.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLHTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          219 c          221 g          284 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctt cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtatgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAJTXY010000296          977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTXY010000296 DAJTXY010000000
VERSION     DAJTXY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.302"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..88
                     /locus_tag="OO566_004298"
     CDS             <1..88
                     /locus_tag="OO566_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607304.1"
                     /translation="LASNGSTLTIQSNGAAFTATTIKIIAEQ"
     gene            88..543
                     /locus_tag="OO566_004299"
     CDS             88..543
                     /locus_tag="OO566_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6607305.1"
                     /translation="MIQFKPTRNIDLIEAVGNHTDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            546..>977
                     /locus_tag="OO566_004300"
     CDS             546..>977
                     /locus_tag="OO566_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6607306.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNN"
BASE COUNT          274 a          240 c          247 g          216 t
ORIGIN      
        1 tcttgcctca aatggcagta cgttaactat tcagtcaaat ggtgcggcgt ttactgccac
       61 aaccatcaag ataatcgcgg agcagtgatg attcagttca aaccaacgcg aaacatcgac
      121 ctgatcgaag cagtaggaaa tcacactgac attatcgccg ggagcaacaa cggcgatgga
      181 tacgactaca agcctgaatg ccgttacttt gaggttaacg tgcacgggca gttcggcgga
      241 attgtttact atcaggagat tcagccgcta acctttgatt gccacgccat gtacctgcca
      301 gagattcgtg gattcagcaa ggaaatcggg ctggcgttct ggcgatacat tctgactaac
      361 accaccgttc agtgcgtcac atcgttcgct gcgcgcaaat tccgccacgg tcagatgtac
      421 tgcgcaatga ttggccttaa gcgtgtagga accatcaaga aatacttcaa aggcgtggat
      481 gacgtgacgt tttacagcgc cacacgcgaa gaactaatcg acttcctgaa tcacgggaga
      541 tagccatgtt atatgcattt aagctgggca gaaaactgcg cggcgaggaa ccttattgcc
      601 ctgaaaaagg cgggaaaggt ggcagctctg ataaaagcgc aaagtatgca gcagaagctc
      661 agaagtatgc cgcagacctg caaaatcagc agtggcagac gatcatgaaa aaccttgctc
      721 cgttcacgcc tcttgcggag cagtatgtta accagcttca gaacctttcc agtttagaag
      781 gtcaggggca ggcacttaat cagtattaca actctcagca gtataaagac cttgcaggtc
      841 aggctcgtta ccagagtctt gctgctgcgg aggcgacggg tggacttggt tcgacagcca
      901 caagcaatca actggctacg atcgcgccga ctctcggtca gtcttggtta tcaaaccaga
      961 tgagcaatta caacaat
//
LOCUS       DAJTXY010000297          957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTXY010000297 DAJTXY010000000
VERSION     DAJTXY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.303"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..957
                     /locus_tag="OO566_004301"
                     /pseudo
     CDS             1..957
                     /locus_tag="OO566_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          262 a          240 c          218 g          237 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga gattccacaa acccagcaag
      841 gggacattgg cgcgaaagat attccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagc
//
LOCUS       DAJTXY010000298          949 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTXY010000298 DAJTXY010000000
VERSION     DAJTXY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.304"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            101..>949
                     /locus_tag="OO566_004302"
     CDS             101..>949
                     /locus_tag="OO566_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6607307.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLMVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSA
                     TISAPEH"
BASE COUNT          320 a          193 c          162 g          274 t
ORIGIN      
        1 caatacctgg agaaagagta caaagcaaac tgtattaaac ttaataagtt atattgtatt
       61 tcactagagt tcttatcgtt gataagaaat tctggttata atgattaaat caacaaatat
      121 acaggtaatc ggttctggta ttatgcatca aataaacaac atacactcgt taactctatt
      181 ttctttgcct gtgagtttga gtccatcttg taatgaatat tatttaaaag tctggagtga
      241 atgggaaaag aacggtaccc caggcgagca acgcaatatc gccttcaata ggctaaaaat
      301 atgtttgcaa aatcaagggg cggaattaaa tctatctgag ttagatttaa aaacattacc
      361 tgatttaccg cctcagataa caacactgga aataagaaaa aacctattaa catatctccc
      421 tgatttccca ccaatgctta aggtaataca tgctcaattt aatcaactgg aaagcttacc
      481 tgccttgccc gagacgttag aagagcttaa tgtgggtgat aacaagataa aagaattacc
      541 atatcttcct gaaactctaa ctcatttacg gatccataat aaccgattgc atattctgcc
      601 actattgcca ccggagctaa aattactgat ggtttctgga aacagattag acagcatccc
      661 cccctttcca gataagcttg aagggctggc tctggctaat aattttatag aacaactacc
      721 ggaattacct tttagtatga acagggctgt gctaatgaat aataatctga caacacttcc
      781 ggaaagtgtc ctgagattag ctcagaatgc cttcgtaaat gttgcaggta atccattgtc
      841 tggccatacc atgcgtacac tacaacaaat aaccaccgga ccagattatt ctggtcctca
      901 aatatttttc tctatgggaa attctgccac aatttccgct ccagaacac
//
LOCUS       DAJTXY010000299          929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTXY010000299 DAJTXY010000000
VERSION     DAJTXY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.305"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            373..>929
                     /locus_tag="OO566_004303"
     CDS             373..>929
                     /locus_tag="OO566_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6607308.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          329 a          180 c          154 g          266 t
ORIGIN      
        1 gtttaagctc cattttcaaa aaaatatatc agggtttgtt actaataact tctgaaaaca
       61 gaaaaaaata gagcggtaat tctatttcgg agaggagtaa ctccatctat cctcgatacg
      121 tactgaacgt tatcgtgata tattcatcat gctttcttca ccattattcg agtataggga
      181 gagacgcaat gaccttaaat acctttttat ggctgaatta ccattctcag gaataacagc
      241 agactccttc ccatagacgg cttaggggga aagcaatacc cggagaaagg gtacaaagca
      301 aactgtttta aaattaataa gccatattct atttaaccag aattcttatc gttgataaga
      361 attctggtta taatgattaa atcaaccaat atacaggcaa tcggttctgg cattatgcat
      421 caaataaaca atatatactc gttaactcca ttatctttac ctatggaact gactccatct
      481 tgtaatgaat tttatttaaa agcctggagc gaatgggaaa agaacggtac cccaggcgag
      541 caacgcaata tcgccttcaa taggctgaaa atatgtttac aaaatcaaga ggcagaatta
      601 aatttatctg agttagattt aaaaacatta ccagatttac cgcctcagat aacaacgctg
      661 gaaataagaa aaaacctatt aacacatctc cctgatttac caccaatgct taaggtaata
      721 catgctcaat ttaatcaact ggaaagctta cctgccttac ccgagacgtt agaagagctt
      781 aatgcgggtg ataacaagat aaaagaatta ccatttcttc ctgaaaatct aactcattta
      841 cgggttcata ataaccgatt gcatattctg ccactattgc caccggaact aaaattactg
      901 gtagtttctg gaaacagatt agacagcat
//
LOCUS       DAJTXY010000300          909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTXY010000300 DAJTXY010000000
VERSION     DAJTXY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.306"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..909
                     /locus_tag="OO566_004304"
                     /pseudo
     CDS             1..909
                     /locus_tag="OO566_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    179..295
                     /locus_tag="OO566_004304"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          211 a          221 c          280 g          197 t
ORIGIN      
        1 ccattgctcc aaagactggc tgtacgccgg agactctgcg tgtctgggtt cgccagtatg
       61 agcgggatac cgggggcgga gatggagggc tcaccagtgc tgaacgtcag cgtctgaaag
      121 agctggaacg tgaaaatcgt gaactgcgcc gcagcaacaa tatccttcgc caggcttccg
      181 cttattttgt gaaggaggag ttcgaccgcc tctggaaaaa atgatgccac tgctggataa
      241 gctgcgtgag cagtacggag tcagaccggt atgcagcgaa ctgcatattg ccccgtcaac
      301 gtattaccat tgtcagcaac agcgacatca tccggataaa cgcagtgccc gtgcgcagca
      361 cgatgactgg ctgaagaaag agatacagcg cgtatacgat gaaaatcatc aggtgtacgg
      421 tgtgcgtaaa gtctggcgtc agttgttacg ggaaggaatc agggtggcca gatgtacagt
      481 ggcgcgcctc atggcggtta tgggacttgc cggtgttctc cggggtaaaa aggtccgcac
      541 taccgtcagc cggaaaaccg ttgccacagg tgaccgcgta aaccgtcagt tcgtggcaga
      601 acgtcctgac cagctgtggg tggctgattt tacttacgtc agcacatggc agggcttcgt
      661 ctatgtggcg tttatcattg atgtgtttgc cggatacatc gtggggtggc gggtctcatc
      721 gtctatggaa acgacattcg tgctggatgc gctggagcag gcgttgtggg cccgtcgtcc
      781 gtctggcacc atccatcaca gcgataaagg ctctcagtat gtgtcactgg cctatacgga
      841 gcgactaaaa gaagccggat tactggcatc aacagggagt acaggcgact cgtatgacaa
      901 cgcgatggc
//
LOCUS       DAJTXY010000301          908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTXY010000301 DAJTXY010000000
VERSION     DAJTXY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.307"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..>908)
                     /gene="ospC2"
                     /locus_tag="OO566_004305"
     CDS             complement(<1..>908)
                     /gene="ospC2"
                     /locus_tag="OO566_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6607309.1"
                     /translation="LDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNAN
                     LSNNTLNIKSFDKIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTH
                     HTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYV
                     HGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFV
                     FQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGN
                     AKSDMALYLLSKFNFTKQDVAEMEKM"
BASE COUNT          292 a          146 c          145 g          325 t
ORIGIN      
        1 ttcattttct ccatctctgc gacatcttgt tttgtaaaat taaatttaga tagtaaataa
       61 agtgccatat cagattttgc attgcctaga gaataagcaa gagcttgatg agccattttt
      121 ttatcagtca ccttattgtt aatttcttca taattggatg ctttaatggc atcctccaaa
      181 gatatatctc tgagtcttat ctttttgaag ttatctgtgc ttagcattct tggtatatga
      241 tactcaagct gaaacacaaa gtttataatt ctatccagac taacaggatc aatgttttta
      301 ttataacaaa actctcggaa tctttgatct ttactctttc ttataaaatc aataagatgc
      361 aacccaattc caagcctcat atcttttgta ttaaatattg gcacatcagt ttttccctga
      421 ttaccatgca catacctgct aacttcatcc acaacctctt taaagttatc taagaaaaat
      481 gattggtgct catggtaaaa aacaggtgga atagcattat caaagtggtc ggttaatgtc
      541 atatatccat atggagagtc atgatcaatg atatacgcat tagcaccaaa atccacagta
      601 tgatgtgttt tatttagtgg gagtgtttct tgatgattgg aaatttcaac cccaaaaaaa
      661 acaaaatcgt ggttagctat ttcttcaata tcctcagaga atgtgttttt atatgtttgt
      721 atattctcag acttaatttt atcaaaggac ttaatattaa gagtattatt tgacaggtta
      781 gcgttacttt gatgtctgaa gttaaaatca gaattaataa taatagagag caagtgacgt
      841 tcatccgaag aaagaggatg agttttttgt attatttcag aaagatagtg tctggacacc
      901 ttatcgag
//
LOCUS       DAJTXY010000302          895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTXY010000302 DAJTXY010000000
VERSION     DAJTXY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.308"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            4..249
                     /locus_tag="OO566_004306"
     CDS             4..249
                     /locus_tag="OO566_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6607310.1"
                     /translation="MKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMSYVFE
                     SALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            236..784
                     /locus_tag="OO566_004307"
                     /pseudo
     CDS             236..784
                     /locus_tag="OO566_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          225 a          207 c          251 g          212 t
ORIGIN      
        1 ctgatgaaag ccatgtcgaa cattactgac tgttccgttc cggatattaa cctgatgctg
       61 agatttatgt tcggaaaaaa acgccgggct tatgttctga ataatggcgg gctgaggatg
      121 agttacgtct ttgagtccgc gctctcgttg gcagaactgg cgattatcca gtcgtcgggt
      181 gcactgccat ccccgccggg tgtttatgtt tcagtagttt taaaggagtc ccgtaatgaa
      241 ggccagtgat aaaccccgcc agctggcggt cccctttgcg agtaccggag ataaaaaccg
      301 tatcccggac aaggcgacac agcagaccag agagagcggt aatgctgcgt atgattcagg
      361 ttttcctccg gtgaccatga cagcggtctc agcgggaggt ataccgccac acggcaagga
      421 ttttaacggt ctgatgtacg atattaccgc agcaatacgg ttcgcccagg ctggcggttt
      481 gtacacgtat aatgccggtt ttgcgggggc cattggtgga tatgcaaaag gagccattct
      541 cgccggagtc gcaacaacag cggtctggct gaataccacg gacgataacc tgaccgatcc
      601 tgaaggctcc gacagtgcgg gctgggtaaa tcttcttgag gatccgaaaa ggatattcct
      661 gcggcagaag aacaatctgt cagaccttca gaataaaggg acggcacggg ataatcttca
      721 ggtttacagt aaagagcagt cagatcaacg ctatgttcat cgggaaggcg ataaaataac
      781 cggagagctg aaaatccgtg gtgttaatgc gctgaggatt ttcaacgaag cttttggcct
      841 gatttttcgt cgttcggaag agtgcctgca ccttattccc accagtgaag gtcag
//
LOCUS       DAJTXY010000303          874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTXY010000303 DAJTXY010000000
VERSION     DAJTXY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.309"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..783)
                     /locus_tag="OO566_004308"
     CDS             complement(<1..783)
                     /locus_tag="OO566_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6607311.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
BASE COUNT          247 a          140 c          238 g          249 t
ORIGIN      
        1 gcgagtcgac aatggattgt tataaatatt aacatctgca taaatgtgca gattcatgat
       61 actgtctggc aatgtgttca gctgattgtt agggatatat agtgattgca gacgttgtgg
      121 aagttcgggc agtctgtgta gttggttttc cccaacgttt aaaagcctca gattttgagg
      181 aaggggcggt agctcttgta atctgttatc aaaagcactt agttcctgta gttcacatgg
      241 taattctggt aaggcagtaa gctggttgtt gccaacagaa agtttttgta gagccggggg
      301 aagatcaggt agcgtctcca ggctattgag aagggctgag agtgactgca aggaagatgg
      361 aagagaaggc aaacatgtta aaactctgtt aagtgaaaca tcaagagcga ctaggtgagg
      421 cggaagagcc gggagcctgc tcagtctgtt atcgctggct ttaagtacag ttaaggacgg
      481 agggagttct ggcagagagc gtagctcatt gctagagatg ttaagttctt gtatgtgcgg
      541 gggcaggtat gggagagagc gtaatctgag taaacttaaa ttgagggctg gctcttgaaa
      601 agccagacat attttcagta atcgaactgc ctgtgttcgg tcttctgttg cagcaccttc
      661 cttggcccag ttatcccaga tgcggtgata atccgtgata ttttgctctt ctacggataa
      721 acgggatata catcctaaac cacaaaagga gcggaaaaaa gaaggattgt ggacaggttt
      781 catttattat tgatcctcat tgtttatata ttaatatatt cgtgcaaatg gagtgaggat
      841 tctttaaaaa aacgcgcctg taaatagtta agaa
//
LOCUS       DAJTXY010000304          861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTXY010000304 DAJTXY010000000
VERSION     DAJTXY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.310"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            515..>861
                     /locus_tag="OO566_004309"
     CDS             515..>861
                     /locus_tag="OO566_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR6607312.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQA"
BASE COUNT          246 a          155 c          209 g          251 t
ORIGIN      
        1 cggaatgcat tatcaataga ggtaaagtcg caaccccaaa tcgtaaagga aaccgtagca
       61 cgtcgtatgc aagaacgtgc cacggctggc tgatggatgt tcgatagcgc gagtttgaat
      121 gaaaatcagc cggagatgat tttacataat tgctacggaa ttattcaata caggaattgc
      181 ttgcgtctgc atggattgac ctgaaatatt cccgaaaatt tctctaaaaa actcgaaaaa
      241 aatggtaact ggttgaatgt attaatatgc aatggtacgt gtcagggatt aaaagatgaa
      301 cgtaaattta ttcaacgcat taattttaaa gggttttatt gtttgttgac gaaaacagga
      361 atcatgttcg gtctcttttt atctgttaaa agccagaagc atttccttcg ctgactttat
      421 agtcaaccat aacacacact ctactgtctg agtccagcgt tttttaacat tcttgttaag
      481 attatgtgat ctttagcgcg ggaggaaaat attgatgaaa cagcctgcgc ccgtttatca
      541 gagaattgcg ggtcatcaat ggcgacatat ctggctttct ggcgatatac acggttgtct
      601 tgagcagttg cgccgcaaat tatggcattg tcgttttgat ccgtggcgag atttacttat
      661 ctcagtggga gacgttatcg atcgtgggcc gcaaagttta cgttgtctgc agttactgga
      721 acaacattgg gttcgtgcgg taagaggcaa tcatgaacag atggcgatgg atgcgctggc
      781 attccagcag atgtctttgt ggttgatgaa tggcggcgac tggtttattg cgctggcaga
      841 taatcaacag aaacaagcga a
//
LOCUS       DAJTXY010000305          850 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTXY010000305 DAJTXY010000000
VERSION     DAJTXY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.311"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            58..555
                     /locus_tag="OO566_004310"
                     /pseudo
     CDS             58..555
                     /locus_tag="OO566_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            596..787
                     /locus_tag="OO566_004311"
                     /pseudo
     CDS             596..787
                     /locus_tag="OO566_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          219 a          210 c          249 g          172 t
ORIGIN      
        1 cgccagcccc gtcccgcccg atagagcgcg gcatagcggg ccccggatta cttgcgtgcg
       61 acggcagaag aagccctgaa gcgaatcagt gaactgtacg ccatcgagga tgaaatacgg
      121 ggattaccag agtcagagtg tcttgcagtc aggcagcagc gaagcaaagc gttactgacg
      181 tcgctgcatg aatggatggt ggagaagaat ggcacgctgt cgaaaaaatc cagactgggc
      241 gaagcattca gctatgtact gaatcagtgg gacgccctct gttattacag tgatgacggt
      301 ctggcggagg cggacaataa cacagcggaa agagcgcttc gtgcagtctg tctcggaaag
      361 aaaaattacg tgttcttcgg tagcgatcac ggcggcgagc gtggtgcact gctgtacggg
      421 ctgatcggca cctgccgtct gaacggtatc gatccggaag cgtatctgcg ccatattctg
      481 agcgtactgc cggaatggcc ttccaaccga gttgacgacc tcctgccatg gaaggtggtg
      541 ctaccatcag gataatcgtc aatacggtgt tcggtgaacg tttacttgga gcatcatgct
      601 tcatgaagaa aaactggcac gtcatcaacg taaacaggcg atgtataccc gaatggcagc
      661 cttcccggcg gtgaaaatgt tcgaagagta tgacttcacg ttcgccaccg gagcaccgca
      721 gaagcagctc cagtcgttac gctcactcag cttcatagag cgcaatgaaa acatagtctt
      781 actggggaca tctgacatca caaatccccg ggtggggatt tgtgtataag agacagccca
      841 tggaggaagg
//
LOCUS       DAJTXY010000306          849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTXY010000306 DAJTXY010000000
VERSION     DAJTXY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.312"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(1..309)
                     /locus_tag="OO566_004312"
                     /pseudo
     CDS             complement(1..309)
                     /locus_tag="OO566_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          280 a          153 c          155 g          261 t
ORIGIN      
        1 ccggccagat ttttatttct attagttgtc gtcacaatac tgaatgcgta cgaccacagt
       61 attctggctc ctgtgtggtt atgctttaat tctgcgttcc gggcagataa gcagttgctt
      121 gcaggaatcc ttcttgtgtt aatgtcagtt ccccttttta ccagtgctga tttccacatt
      181 ccgtccaaca gagcttatag cctttccctg gattatagca ttgtccggct gaagttcttt
      241 ttgaataata atagaagcac tgctggcaga tccagtccgt ttttcataac ccactgtact
      301 gataaccata atctaatcag tagaaattga gtcgaaaata agcactactc catacaggat
      361 aattagaggt cagtttgatt attcacaatt catcatcagc attttctatt tctgacgaaa
      421 tcaacatgaa aataaccata tatgataatt attataataa cggctttaat tggaatacat
      481 atattacaac gtattatata taattggtat tctgggaact atattctcaa aatacagtag
      541 aaacgaggta tgtttctggt ggaaaggaca gtgggattaa aaagtaagaa ttgataaaaa
      601 aaacgccagc aacacatagt gccggcggag ggaatacccc atggagaaaa tgtgatgcct
      661 agagcatcat gaatatataa ttaaaaatag ttagcgttgt caatactttc acaaaaataa
      721 ttttcgtagt atagaaagat atttttatgc atgacctacc tgaatttgct ccgggtagag
      781 gttataaata aaaattgaat cacgacaaac acaatattca tagtatggcg atgcctacgc
      841 cagcaagaa
//
LOCUS       DAJTXY010000307          843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTXY010000307 DAJTXY010000000
VERSION     DAJTXY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.313"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..764
                     /locus_tag="OO566_004313"
                     /pseudo
     CDS             1..764
                     /locus_tag="OO566_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          227 a          182 c          200 g          234 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcgctgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagcagg ttggatttgc ccctatattt
      781 ccagacacct gttatcactt aacccattac tggcccgctg ccgtagatat tcccgtggcg
      841 agc
//
LOCUS       DAJTXY010000308          828 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTXY010000308 DAJTXY010000000
VERSION     DAJTXY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.314"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..227
                     /locus_tag="OO566_004314"
     CDS             <1..227
                     /locus_tag="OO566_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607313.1"
                     /translation="AERIAKELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGE
                     NLRSATHSAGRSRAAIVADLESGGNWMDSL"
     gene            311..>828
                     /locus_tag="OO566_004315"
     CDS             311..>828
                     /locus_tag="OO566_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607314.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGAEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTV"
BASE COUNT          213 a          226 c          203 g          186 t
ORIGIN      
        1 aagctgagcg tatcgctaaa gagctagcgt ctcagaaaga gcgtacccgc gaaatgttca
       61 tgaccgaaga cgaatggcaa aaagagctac agcgccgcga acaggtcaaa gctgaacgcc
      121 ggacctatgg tgaaaacctc cgcagcgcta ctcattccgc tggtcgctca cgtgccgcta
      181 tcgtcgctga ccttgagtca ggcggtaact ggatggacag cttgtaagca agcgtccctc
      241 atctgagaac acacggtccc tgactgcctg ttagggactt caagtatttg actcaaggag
      301 attaattata atgaatatga ctaaaaacgc tttccagttt ggcattgagc ctgttcatat
      361 caccgacaac gacaaccttc aggttaatga gggccttccg actaacgccg accctcaggt
      421 ctatgccctc cagttagcta agactgttaa ggctatgctg aacggtgtac ttaaagacgc
      481 tcaagataac atcccgttcc ctgtagaagt cctgccgacc cgtaacagct taccgactcc
      541 aatcattgct cacactctgg ctgaccgctc tgtgctggtc cctgtccgtg gtggtaagcg
      601 acctgaagtt gtgaccgctc cgtctggcgc tgaaatcacc gttgagccga ttgaacaggc
      661 gatcctcgtg tctcaccaaa caaagctttg ggaccagaag tcgaccactg gtttcactca
      721 ggggacgctc cagcaggacg cactgaacat ctgcgataac gtgattcgta ccattaactc
      781 taagatggtt gacgtgctgg agtcctcaaa gctgctgaag accgtaga
//
LOCUS       DAJTXY010000309          816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTXY010000309 DAJTXY010000000
VERSION     DAJTXY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.315"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            45..776
                     /gene="rlmB"
                     /locus_tag="OO566_004316"
     CDS             45..776
                     /gene="rlmB"
                     /locus_tag="OO566_004316"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6607315.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT          196 a          209 c          232 g          179 t
ORIGIN      
        1 ataaacgtag tgcatcaggc aaaacgtaaa caacgagtac attaatgagc gaaatgattt
       61 acggcatcca cgcagtgcag gccctgctgg agcgcgcccc tgaacgtttt caggaagtct
      121 ttattttaaa aggccgtgaa gataaacgtc tgttaccgct gattcacgcc cttgagtctc
      181 agggcgtggt tatccagttg gcaaaccgcc aatatctcga cgagaaaagc gacggtgccg
      241 tgcatcaggg cattatcgcc cgcgtgaagc caggacgtca gtatcaggaa aacgatctgc
      301 cggatctgat cgcttcgctc gatcaaccgt tcctgctgat cctcgacggc gtaaccgatc
      361 cgcacaacct cggcgcgtgc ctgcgtagtg cggacgccgc aggcgttcat gcggtgattg
      421 tgccgaaaga tcgctccgca cagctcaacg ctacggcgaa aaaagtagcc tgcggcgcgg
      481 cagaaagcgt tccgctgatt cgggtgacta accttgcgcg caccatgcgt atgttgcagg
      541 aagagaatat ctggatcgtc ggtacggcag gcgaggcgga tcatacactc tatcagagca
      601 aaatgaccgg acgcctggcg ctggtgatgg gcgcggaagg tgaaggtatg cgtcgcctga
      661 cccgtgaaca ttgcgatgag ttgatcagca tcccaatggc aggaagtgtt tcttccctga
      721 acgtttcggt tgcaaccgga atttgcttat ttgaagcggt tcgtcagcgc agctagttcc
      781 tctgaattat gaaaagccat ccagatttgg atggtt
//
LOCUS       DAJTXY010000310          803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTXY010000310 DAJTXY010000000
VERSION     DAJTXY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.316"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..679)
                     /locus_tag="OO566_004317"
     CDS             complement(<1..679)
                     /locus_tag="OO566_004317"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6607316.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDS"
BASE COUNT          218 a          165 c          217 g          203 t
ORIGIN      
        1 gactatcagg atcgaggatc tggctgcatc cccaacgaac gaattcatcc acataacgtg
       61 tgtagttggt aaacaagacg aacggttgaa aatgttctac tggcgtaccg gtataatggc
      121 gcaaccgtgc gagggaaaaa tcgacgcggc gcgcatcaaa atgcgatagc ggggagaatt
      181 ccgttggatg ataaatgccg tctgcagttt catcgccgat ttgcgccagt tctgttgtcg
      241 ggaagtagcg agttaaatca gcgctcattg agcgatcaag tgtcaattca gagccatcga
      301 tgacataagg ataagggatt tcatgttgcg agggttgcac agagatatgc gcaccataat
      361 cctgataaag caacgttagt tgctcattca gatacgaacg aaagagagta gggcgagtaa
      421 tcgtggtggt gtagctgcct gcgtgagtaa aacgaccaaa tgcgcgcgtt ttggggggat
      481 ttgttgtgct accgtcccag gttacggtca gtgatggata gacaaaaaga ccttgtttac
      541 gggcgttttc atcaggtaat tcgccaattg taatatattt gccaatggcg ttgcgtaaag
      601 caactacaga ttgctcatac agcgcgtcga gtttatccag tgcctgagct ggggtcagac
      661 cggagccctt attattcatg tgtgtctcct gttccataca attaccgcac atccgatagt
      721 atgtcacagg aatgtgaaac aaaagtataa ggcgaagatc ggatgatctt cggcaggcta
      781 tcacgagcgt tgattgtctt tca
//
LOCUS       DAJTXY010000311          787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTXY010000311 DAJTXY010000000
VERSION     DAJTXY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.317"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            126..787
                     /locus_tag="OO566_004318"
                     /pseudo
     CDS             126..787
                     /locus_tag="OO566_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          211 a          203 c          221 g          152 t
ORIGIN      
        1 cggcccgtga tgatgcacaa cgttttgcgg agaaagcccg acaggatgca accgtcacag
       61 ctgaggacag aaaggccact gcggaagatg tgacaagcac aggagcaaat gcagccgcag
      121 ccggacagag cgcacaggat gccgcaggtt atgcccgcgc agcagaacag gccaaaaatg
      181 acattgatgc tgcgctgacc ggcaccctga aaacggctaa ccatctgtct gaaatcgcag
      241 cagcaggcga aaaggcacaa cagaagtccc gggataatct ggggctgaaa agtgcggcca
      301 cgatggaagc acagagcgac atttacgacc ggacaaaagg ccgtctggcg atacccggcg
      361 cattcggctt tgggtgtgct tttctgcctg aagatgttat ccgttttgac actaagagtg
      421 atttcctggc ctgggtaagg aatgcgctgc caggtgaata ttccgttgct ggcccttacg
      481 gcatcatcat acccgacaca cggtttgaag gggtgctctg catccggtgg actgatgcac
      541 gccctgagac aacagaaccg cggtacagag ccaaatccct tactttttac ggcattaacg
      601 gccccattta tcatacccgc tactgctact ggcccatatc cagactgact ggctgggtga
      661 aaataaatat aaccacagaa gatattattt acagaatcgt ggcgagctct gtccgcaaca
      721 gatggggaga ccctgacatt ggcgggctga ttattgctgc gtaccaggga gaagctgacg
      781 gtgataa
//
LOCUS       DAJTXY010000312          771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTXY010000312 DAJTXY010000000
VERSION     DAJTXY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.318"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            26..771
                     /locus_tag="OO566_004319"
                     /pseudo
     CDS             26..771
                     /locus_tag="OO566_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          199 a          185 c          216 g          171 t
ORIGIN      
        1 cagacaacca gtatcactta tttaagtgat agtcttaata ctagttttta gactagtcat
       61 tggagaacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt gtagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtggccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagctt agtcagccgt tgtctccggg tgtcgcgtgc gcagttgcac
      481 gtcattctca gacgaaccga tgactggatg gatggccgcc gcagtcgtca cactgatgat
      541 acggatgtgc ttctccgtat acaccatgtt atcggagagc tgcccacgta tggttatcgt
      601 cgggtatggg cgctgcttcg cagacaggca gaacttgatg gtatgcctgc gatcaatgcc
      661 aaacgtgttt accggatcat gcgccagaat gcgctgttgc ttgagcgaaa acctgctgta
      721 ccgccatcga aacgggcaca tacaggcaga gtggccgtga aagaaagcaa t
//
LOCUS       DAJTXY010000313          771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTXY010000313 DAJTXY010000000
VERSION     DAJTXY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.319"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>771
                     /locus_tag="OO566_004320"
     CDS             <1..>771
                     /locus_tag="OO566_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR6607317.1"
                     /translation="NYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYL
                     LKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVD
                     ESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLA
                     VDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNREGNYNII
                     DSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKL"
BASE COUNT          240 a          134 c          163 g          234 t
ORIGIN      
        1 ttaactatca actggtatcg gaacgcgcgg taaaacgtgc cgatagccgc tttgaactta
       61 ttcagaaaaa cgttggctat ttctttaaag atattgaacg ttcggccctg acattaaagg
      121 actcactgta tttattaaaa aatacagagg agattcaacg cgccgtgatt ctgaaaatgg
      181 aaatgatgcc atttttagac tcggtgggac tggtacttga tgataataaa tattatctct
      241 tttcgcggag ggcgaatgat aaaatcgttg tttatcatca ggaacaagta aatggaccgc
      301 ttgtcgacga gtcagggcgg gttatttttg ccgattttaa cccatcgaaa cgaccgtggt
      361 cggtggcttc agatgactct aacaacagct ggaatccggc atacaattgc tttgatcgtc
      421 cgggtaaaaa atgtatctct tttacgctac gtatcaacgg caaagatcac gatttgttag
      481 cggtagataa aattcatgtc gatttaaact ggcggtatct gaacgagtat cttgatcaaa
      541 tcagcgctaa tgatgaagtt ctatttttga aacaaggtca tgagatcatt gccaagaatc
      601 aactcgctcg tgaaaaactg attatttata atagagaagg taattataat attattgatt
      661 ctgtcgatac ggagtatatt gcaaagacat cagcagtgcc aaataatgca ttgttcgaaa
      721 tctattttta ttatcctggc ggtaatttat tgaacgcatc agataaactt t
//
LOCUS       DAJTXY010000314          759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTXY010000314 DAJTXY010000000
VERSION     DAJTXY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.320"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..265
                     /locus_tag="OO566_004321"
     CDS             <1..265
                     /locus_tag="OO566_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6607318.1"
                     /translation="NILGSFFTTDILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQF
                     ASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKITYN"
     gene            complement(308..508)
                     /gene="glgS"
                     /locus_tag="OO566_004322"
     CDS             complement(308..508)
                     /gene="glgS"
                     /locus_tag="OO566_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6607319.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          236 a          170 c          132 g          221 t
ORIGIN      
        1 caatatttta gggagctttt tcactacgga tattttgagt gattatagcc atctggatat
       61 gggtaatgga ctgctattga agatatttca taaagatgga acagcaacgg aatttaaccg
      121 cttctcacaa tttgcttctt tttcatcgtc tagtgcacct tcggtcaccg caccattcag
      181 ggcagaactt agtgcgaacc cggcagagac tgttgttgag ggaccgttta gtaaagacgt
      241 aatcctcaaa atcacctata actagcatct aatacaaaca ctaaaacggg ccatcaggcc
      301 cgttatatca gtgctctaac tccagctctc ttgcctgcat catctgttgg caataccagg
      361 cataacgtgc gcaaaaccag gttctatgtt cttcatccat gttaccagta acggccagaa
      421 tatcgaccaa gcgaccttct gcgtgcattc tggcaaaact acgcgccagg aaatcgaaat
      481 tattcaaaga attaagacta tgatccataa gcacatcctc cggttatcgc atacctttta
      541 aggccatatg ctttttctga tttcagtatt ccccaatcag tgtagtgcct ttaaacataa
      601 cgtgcgtaaa tatattgtcc ccgatcacac tttttagtga aaaataaaaa accatattaa
      661 aatactttct cttccagaac ttctctaaca tgtgttatta ttaacaaaga aaaatgtaag
      721 aagggaaact attccaaggt aaatattgcg tcactaaat
//
LOCUS       DAJTXY010000315          750 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTXY010000315 DAJTXY010000000
VERSION     DAJTXY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.321"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>750
                     /locus_tag="OO566_004323"
     CDS             <1..>750
                     /locus_tag="OO566_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6607320.1"
                     /translation="DNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQQTK
                     NLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRTIHL
                     VQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGPHYK
                     VDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNV
                     YGAWMKDNNHEQIELLNRRLSESVPCMPHKKA"
BASE COUNT          250 a          166 c          161 g          173 t
ORIGIN      
        1 agataatggt tatacatcag gaaatccatt taacggtctg acaccattaa aaaagtccaa
       61 gatagaacca gatcccctca cccgtgacga attcattcgt tttattgagg cttgccgtca
      121 tcaacaaaca aaaaacctgt ggattatcgc tgtatacacg ggtattcgtc acggggagct
      181 ggtatcactg gcctgggaag atatagacct taaagcgagg actataacca tccgccggaa
      241 ttatacaaaa ctaggcgaat tcactccacc aaaaactgat gctggtaccg gaagaacaat
      301 tcatctagtt caaccagcta ttgatgctct taaaagccaa gcggaaatga ccatgcttgg
      361 aaaacagcat tctgtagagg taaagcagag ggaatatggg agaagtactg tgcataaatg
      421 cacttttgtt tttagtcctc aggtaataaa acagcggcag ttttccggac cgcactataa
      481 ggttgactcc atcagggagt catggacaag tatcttaaaa cgcgcaggtc tgagacacag
      541 aaaatcgtat caatccaggc atacctatgc atgctggtca cttgccgcag gagctaatcc
      601 tagttttatc gcaagccaga tgggccacac aaacgcacaa atggtattca atgtttacgg
      661 agcatggatg aaagacaaca atcacgaaca gatagaactt cttaacagaa gactatctga
      721 aagtgtccca tgcatgcccc ataagaaagc
//
LOCUS       DAJTXY010000316          748 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTXY010000316 DAJTXY010000000
VERSION     DAJTXY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.322"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            8..73
                     /locus_tag="OO566_004324"
                     /pseudo
     CDS             8..73
                     /locus_tag="OO566_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            301..549
                     /locus_tag="OO566_004325"
     CDS             301..549
                     /locus_tag="OO566_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6607321.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(665..>748)
                     /locus_tag="OO566_004326"
     CDS             complement(665..>748)
                     /locus_tag="OO566_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6607322.1"
                     /translation="KKYRVLLNRVDTSTAPDIEWPEEPDTM"
BASE COUNT          217 a          165 c          165 g          201 t
ORIGIN      
        1 tgagacgatg gcgctttaca caattggtga agtggcgttg ctttgtgata ttaatcctgt
       61 cacgttacgc gcgtgacgca acatttttaa aaatcgacga agatcgtcca ggagcgccgt
      121 ttttcaaggg ttggatagat gattcgttta ccatgtcgac gaacttcgac gaatttcgcc
      181 gataaaaagt gtcccataca tgccccattg atgccccata aacaatatca ggaattgaca
      241 ctggttatcc atacagataa aaataacacc tgtacataac tacagttaca ggagggtgtt
      301 atgcgtgttg agatctgtat cgctaaagaa aaaatcacta aaatgccaaa cggtgctgtg
      361 gatgcgttaa aggaagaatt aacccgacgc atcagtaaac gttatgacga tgtagaggtg
      421 atcgtaaaag ccaccagcaa cgatggcctt tctgttacac gcaccgcaga taaggattct
      481 gcaaaaacat ttgttcagga gactctgaaa gatacctggg agtctgctga cgagtggttt
      541 gttcactaat aaacacgtca aatcggtaac ggctggaaat cattcaatac tcgcactatc
      601 gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc gtgtctgcgg tttttctttt
      661 tcgcttacat tgtgtctggt tcttccggcc actcaatatc aggtgcagtt gatgtatcaa
      721 cacggttcag caacacccga tacttctt
//
LOCUS       DAJTXY010000317          743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTXY010000317 DAJTXY010000000
VERSION     DAJTXY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.323"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..574
                     /locus_tag="OO566_004327"
     CDS             <1..574
                     /locus_tag="OO566_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6607323.1"
                     /translation="PESLSHITTESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLG
                     RYLKKPPIAASRLAHYNGGASLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFK
                     MVRYFGFLASRVCGEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGR
                     MVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          194 a          166 c          246 g          137 t
ORIGIN      
        1 gccggagtcg ttgtcacata tcacgacgga atcacagagg agaagcctgg tgctgaaagc
       61 cggaggaaaa tactggcatg tgtacatgtc gaaaaaaacg gccggcggga ggaatacggc
      121 gcgctacctg ggtcgttatc tgaagaagcc gccgatagcg gcctcccggc tggcacatta
      181 caacggaggg gcgagcctga gcttccgtta cctggaccac aaaacgggag aaacggcgac
      241 ggaaacgctg acacagcgtg agctggtcgc gaggctgaaa cagcacatcc cggagaagtt
      301 ttttaagatg gtgaggtact tcgggttcct tgccagccgg gtgtgtggag agaagctgcc
      361 gcaggtgtac cgtgctctgg ggatggataa accggaacca gtggcgaaag tgtgctatgc
      421 acaaatggtg aaacagttcc tgagtcgtga cccgttcgaa tgcgtgctgt gtggcggccg
      481 gatggtatac cgccgggcca tcgcgggact gaatgtggag gggctgaaga aaaacgcgcg
      541 ggatatcagt ctgctgaggt atatgccggc ctgacacagg ctaagtgtgc ccgggaatcg
      601 gtgcaagtat aaaaaacagc ctgttgcggt ataagaatcg accatgcaga gagagaaaac
      661 accggagtgg agggaaaagc agaaaagctc acgtggcata aggcgtggac agcggtatcg
      721 tctggttttt caatttccta tac
//
LOCUS       DAJTXY010000318          732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTXY010000318 DAJTXY010000000
VERSION     DAJTXY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.324"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..374
                     /locus_tag="OO566_004328"
                     /pseudo
     CDS             1..374
                     /locus_tag="OO566_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator"
     gene            568..643
                     /locus_tag="OO566_004329"
     tRNA            568..643
                     /locus_tag="OO566_004329"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Ile,seq:cat)
     gene            645..721
                     /locus_tag="OO566_004330"
     tRNA            645..721
                     /locus_tag="OO566_004330"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:679..681,aa:Lys,seq:ttt)
BASE COUNT          214 a          145 c          194 g          179 t
ORIGIN      
        1 gggaatatca gatcgcgata acacagtggg cgtggaatga atttaatgct caatccggta
       61 ccagaaaaat tgcagggaaa acgcaggaac gcctgaaaaa attaatctgg ctggcggcgc
      121 aggcagtaaa agcagaactt tttggtgggg aaggttatga ataccaggtt ggtggggaag
      181 gttatgaata ccaggagctg gcatttactg gcgggagtga caactaaaaa ctggtccaaa
      241 acatttactc gtcactggat tgcaatgaaa cacatttttc accgactgga tagtgaggct
      301 ttattgtttg taatgagaac acgttcaaaa aaaagacggc attttcaaag caaagtgttg
      361 caaaagtaga ttgaaaggca tatattcatg caaatctgat atttcgccga ttttttacgt
      421 gatggcaaaa gcaaacaaaa cccgcccaca agcgggtttt ttgtgccact tatctcggat
      481 agacatggtg aatgcgctgg tggaggagat aagggtgatt tttgaatgct tgcaacattg
      541 atttcgtaac gttattatcc tgcgcccggc cctttagctc agtggtgaga gcgagcgact
      601 cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca ccaaccgcca ctagctcatc
      661 aggaaagagc gtcaaccctt taagttgagt gtgcgaggtt cgagtccccg gtggcggtcc
      721 agtgccgact tg
//
LOCUS       DAJTXY010000319          713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTXY010000319 DAJTXY010000000
VERSION     DAJTXY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.325"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..614)
                     /locus_tag="OO566_004331"
     CDS             complement(<1..614)
                     /locus_tag="OO566_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6607324.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQAESEVSTPVHPSASMVGLDAGVAKLASLSDGTVFEPVNSF"
BASE COUNT          169 a          181 c          173 g          190 t
ORIGIN      
        1 tgaaaactgt ttacaggctc aaagactgtg ccatctgaca gcgaggcaag tttagccacg
       61 ccagcatcca gcccgaccat tgatgctgaa gggtgaaccg gagttgatac ttcactttct
      121 gcctgaatac tgatgtacca cttaccgcag gactggctga cagtgacatt tttcacaact
      181 cccgtgacct gacggctgtt ccggtagcgc atccagccaa gtttcggcag aaaaatacgg
      241 ctgttttcct gatcgagctt aacgccctgc gggtagcgga atgcagcatt ctgtccccgc
      301 tttttgaatc ggggaaaagc agcccgcttc cggaagaagt ttttgtaagc ccgctcaagg
      361 tctttcagtg actgttgcaa tggctgtgag ggcgaatctt taagccattg cgtttcagtg
      421 gcgtttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta tttatttccg
      481 gcctcatgat tctcattctg aagcgccaga gcacgattga aaacgaaacg acatgcgcct
      541 gcgaagcgcc tcatttcacg ctcctgttga ccaccgggtc ttaactggaa tttaaatgcc
      601 tgtaatcgtt tcacaggtca aatgatactt tggtctgtga aaaaagaaac cgatatccgg
      661 cgtggaaggc attgtgtttt cctgatgcat gttcacctgg tctttgtcac cag
//
LOCUS       DAJTXY010000320          702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTXY010000320 DAJTXY010000000
VERSION     DAJTXY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.326"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>702
                     /locus_tag="OO566_004332"
     CDS             <1..>702
                     /locus_tag="OO566_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR6607325.1"
                     /translation="PLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKT
                     AQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQ
                     MELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEH
                     RHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKV
                     CHLLTSMTDAMRFPGG"
BASE COUNT          175 a          206 c          199 g          122 t
ORIGIN      
        1 cctcttcacc agattgtgaa tcgcctggac atcatgctgc cgggcaatcg ccccttcgtt
       61 gcccccagtg ccgttattca ggcccgccag cgcctgggaa gtgaggctgt ccgccgcgtg
      121 ttcacgaaaa cagcgcagct ctggcataac gccacgccgc atccgcactg gtgcggcctg
      181 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
      241 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      301 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      361 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      421 atggataaag gttattactc actgggactg ttaaatggct ggagcctggc gggagaacac
      481 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      541 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      601 ctgggaaatg aagtgacagc ccgcctgctg accgtgacgc gcaaaggaaa agtctgccat
      661 ctgctgacgt cgatgacgga cgccatgcgc ttccccggag ga
//
LOCUS       DAJTXY010000321          691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTXY010000321 DAJTXY010000000
VERSION     DAJTXY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.327"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..182)
                     /locus_tag="OO566_004333"
     CDS             complement(<1..182)
                     /locus_tag="OO566_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6607326.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIG"
     gene            376..>691
                     /locus_tag="OO566_004334"
     CDS             376..>691
                     /locus_tag="OO566_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR6607327.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIA"
BASE COUNT          186 a          164 c          167 g          174 t
ORIGIN      
        1 ccgattttat gcccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt
       61 gcgaaaggcg tctcgccata caacaaacgc acatcgggca acagttctga caaactattt
      121 tctttatttt tcagtttatt aactttatcc gccagcaagc gacaaatctc ttcacgtttc
      181 atatcgcgta atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt
      241 tctccagcac tctggagaaa taggcaagac attggcagaa atgagcattg agagctaggg
      301 cgctggcgat cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag
      361 caaagggagc aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt
      421 aaaggcgcaa tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc
      481 tacggtggcg gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga tgccctgaaa
      541 ggcatggacg tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg
      601 aacgccgtga aactggttcg cgaacagaaa gtgactttcc tgctggcggt tggcggcggt
      661 tctgtactgg acggcaccaa atttatcgcc g
//
LOCUS       DAJTXY010000322          683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTXY010000322 DAJTXY010000000
VERSION     DAJTXY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.328"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..467
                     /locus_tag="OO566_004335"
     CDS             <1..467
                     /locus_tag="OO566_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6607328.1"
                     /translation="WGRWSYIGGGKTGNMFNQLLASKKLTKTAVNEALRRMKKAGIEK
                     PELEAFLREMINGKQKTWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMT
                     KRKMAELLNDAHPEWCFRTCCSRVDVWLNLAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT          163 a          147 c          204 g          169 t
ORIGIN      
        1 tgtgggggcg ctggtcgtat attggcggcg gtaagacggg aaatatgttt aaccagttgc
       61 tggcatccaa aaaattgacg aaaacagccg tcaatgaagc cctgcgcagg atgaaaaaag
      121 cgggaataga gaaacctgag ctggaagcgt ttttgcgaga gatgatcaat ggcaagcaaa
      181 agacctggct ggcgcattgt actgatgcag aggcgttatg tattgataga gtcataagtg
      241 aggtgctggc agagcatcct ggattgatta gtgtcctccg gcaacggtat gaagggcggg
      301 ggatgactaa gcgtaaaatg gctgaattgc tgaatgacgc acaccctgag tggtgtttcc
      361 ggacgtgctg cagtcgggta gatgtatggc taaatcttgc tgaatatatg ctctatctac
      421 cgatgcgtga tgcattctct tccggggatc taaaaaccgt ttgttgactc aatctgttat
      481 ccggggctat attcctcacg cgcagcaaaa tctggcgtcg ggattggcgt cccggatgtt
      541 tacggagcga tatgagacgc gcccgcgtct tttttcatat cgtttgcaca gtcacattcg
      601 cgatttatgg cgggctgtgt gggggagccg aaaggctcgc cggtttccgt acccggttac
      661 gccaaccctg cacagttcgc cac
//
LOCUS       DAJTXY010000323          679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTXY010000323 DAJTXY010000000
VERSION     DAJTXY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.329"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..246
                     /locus_tag="OO566_004336"
     CDS             <1..246
                     /locus_tag="OO566_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607329.1"
                     /translation="NIYGHHFGAPHDAADFRRCVALVEQIPEIRDSFDKVAKRVPAFK
                     GILNEWDSLVDLLKSEMKIHGNKAPETYRRISELRKD"
     gene            337..>679
                     /locus_tag="OO566_004337"
     CDS             337..>679
                     /locus_tag="OO566_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6607330.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAVKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTI"
BASE COUNT          211 a          158 c          165 g          145 t
ORIGIN      
        1 aatatctatg gtcatcactt cggcgctccc catgacgcag ccgatttcag acgatgcgtt
       61 gcacttgttg agcagatccc agaaatcaga gattcattcg acaaggttgc aaagcgcgtt
      121 ccggcattca aaggcatcct caacgaatgg gattccctcg ttgatctgtt gaagtctgaa
      181 atgaagatac acggaaacaa agcaccagag acttacagaa gaattagcga gttacgcaag
      241 gactaaccac agcctcatac tcgatgaggc ctgttcattg ctcaatgata tccagaccta
      301 ccgcaataat accaatccaa taaatggaga ttccaggtgg aagaagaaat cttcactcgt
      361 gaagaggcag cgtcgtatct gaaggtagac aaaggcacta tcacgcagtg gatacgaagt
      421 ggacgacttc aggccgtaaa gataaatcca gataaaccta aaagcccata tcgcatttgc
      481 aagtcagact gcattgcggc gcttaagtct gtgagacaca atagcgcggt gaatgcggtt
      541 gatgtgcagg aggttaaagc atgtcaatca aactacgcgg tggcacgtgg cactgcgatt
      601 tcgtcgcgcc agatggatca agagttagac gctctcttga aacatcggac aaaaggcaag
      661 cgcaagaact tcacgatcg
//
LOCUS       DAJTXY010000324          663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTXY010000324 DAJTXY010000000
VERSION     DAJTXY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.330"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>663
                     /locus_tag="OO566_004338"
     rRNA            <1..>663
                     /locus_tag="OO566_004338"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          154 a          146 c          222 g          141 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ccctttaagg gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
      661 agg
//
LOCUS       DAJTXY010000325          627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTXY010000325 DAJTXY010000000
VERSION     DAJTXY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.331"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..>627)
                     /gene="pdeF"
                     /locus_tag="OO566_004339"
     CDS             complement(<1..>627)
                     /gene="pdeF"
                     /locus_tag="OO566_004339"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6607331.1"
                     /translation="KIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVFLFYLPLAL
                     MISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFTPRR
                     NNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGTLIN
                     YQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFA"
BASE COUNT          215 a          137 c          137 g          138 t
ORIGIN      
        1 gcgaactctt ttttggtgac tttggcatca acctgctgtt ttaattgcga ataatagcta
       61 cgtaaataaa atggatttcg cactacccga atgatgaagt agcacagcgg gacaccgatc
      121 agattaccca ccagtaaggc ctgataattg attaaagtcc cgaggttaaa aggcatgaca
      181 ccgaccagat tttctctgct cgccagtaat cctacaaatg cagcaaactg gaaaagtatc
      241 agaaacagcg ttgcaggaaa cactatctgc cagaatatac gctgggaaat taaacgggta
      301 tcaccatgtg aaacgttgtt acgccgggga gtaaagaccc tgtaaccgcc ccaacagaga
      361 atgataatga taataaaatt agccgttaat gagagcgttt cgtaaaaacc cagctctgca
      421 tatttgcgga caaaaatccc taaggtgatc cctggcaatg ccgcccagct gaaaaacatc
      481 atcatgctga tcatcagtgc caaaggcaga taaaagagaa aaacctcacc ggaagaaata
      541 tgcgcgaaag tattaatgtg ggcgaaaatg ggcaagatta aagaaggcag gaacagcgga
      601 agcccccacc atttatcacg tattttt
//
LOCUS       DAJTXY010000326          610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTXY010000326 DAJTXY010000000
VERSION     DAJTXY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.332"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..240)
                     /locus_tag="OO566_004340"
     CDS             complement(<1..240)
                     /locus_tag="OO566_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR6607332.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQVDLVSDAAERYQGQVCTPAHQ"
BASE COUNT          189 a          130 c          127 g          164 t
ORIGIN      
        1 ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc tcactaaatc
       61 tacctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg ttcggagctg
      121 attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa tgatgggaaa
      181 gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac gatgactcat
      241 tgctatcaat ttcctgcctg tttatacccg gccattgcaa ggtgaaaccc tggctaccga
      301 cgtctttaga taaatcttac ggtataaata gcacttttgc ctgatttgaa attgatacct
      361 ttttaatcgt cacctggatg aaaataattc attgatattc aaaattaatt ttctacagga
      421 atacagaggc ggcgggaaga tatgccctta atgccactga aaaaccacat aatctgtaca
      481 gcaataaaag tcacggccta atatggctaa caaacaatca attgtgatat ggttcacaaa
      541 attaatgaaa caaacagagt gtttcatttt tgtgttctgc aagccccgcg atagccagag
      601 tgtaccacgc
//
LOCUS       DAJTXY010000327          604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTXY010000327 DAJTXY010000000
VERSION     DAJTXY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.333"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(43..465)
                     /locus_tag="OO566_004341"
     CDS             complement(43..465)
                     /locus_tag="OO566_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6607333.1"
                     /translation="MWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTP
                     PEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVR
                     ESNGEEQHFSVPFQTPAIALHEGYFKYSVMGGNIKKKV"
BASE COUNT          152 a          172 c          120 g          160 t
ORIGIN      
        1 cgtacatcgc cgtgatttcg cccacggaga caggggagct ttttatacct tcttctttat
       61 attccccccc atcacactat atttgaaata tccttcatgc agagcgatgg ccggcgtttg
      121 aaatggcacg gaaaaatgct gctcctcgcc attactctct ctcactgtaa catcaagctc
      181 cccgccaacg gccagcgttg gtaaattatc cagcgcaaaa ggccctggcg gcacagaacg
      241 ggtaaaaata atttgcccat tttgtttaat aataacctgc gcctcacttt gtgctattcc
      301 atgcaccaca ggtgtgtagt agctgtcctc cggcggagtc atcgcatcgt cggtcgctaa
      361 catcccgccg cgaaaaggta tgctgtcaaa cacctcagac gacgtataag attctccaag
      421 cgttaatcgg cttttaatcg catttatgcc tcgctcaaac cacatgtaac ccctttgcca
      481 tctttctgac tggccaagtt ccttgctcca tgtcactgaa ctacgaaacc tccaggcacc
      541 aagattaaac cctggagtga acctgccata acgagaatta cgcgtggtag attgattatc
      601 attt
//
LOCUS       DAJTXY010000328          597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTXY010000328 DAJTXY010000000
VERSION     DAJTXY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.334"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            64..>597
                     /locus_tag="OO566_004342"
     CDS             64..>597
                     /locus_tag="OO566_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR6607334.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGGIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKAQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRR"
BASE COUNT          162 a          147 c          142 g          146 t
ORIGIN      
        1 catgtgatat tgtgtaccag atgtgtgcca aactattatt tctgagcgta ccaaatatta
       61 tttatggcta taagcgacac aaaactgcgt actatttatg gtaaaccata ttcgggccca
      121 caagaagtgg ctgatgccga tggcctcagc gtacgaattt caccgaaggg gggcatccag
      181 ttccagtacc gctatcgctg gcatggcaag cctaatcgac ttgggcttgg tcgataccca
      241 tccctgtctt tgaaggatgc cagacagatc actgctgact tgcgaaagct ctatttctca
      301 ggaacggatc cacgcaccta ttttgaagag aaggtggaga actccatgac ggtcgcccag
      361 tgtctcgact actggttcga caactacgtc tctacaactc tcagagaaaa ggcccaggca
      421 ctttatcgat caacggttat gaagcgcatg catgacgcct tccctaatcg tccggcatct
      481 tctatcacgg ttaagcaatg ggttgacctg cttaccgaag aagaaaaaga taatccacgc
      541 cgagcaaggc aggtgctaag tcaactaaga tcagcaatta gttggtgcat gcggcgt
//
LOCUS       DAJTXY010000329          591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTXY010000329 DAJTXY010000000
VERSION     DAJTXY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.335"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(1..316)
                     /locus_tag="OO566_004343"
                     /pseudo
     CDS             complement(1..316)
                     /locus_tag="OO566_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            complement(318..>591)
                     /locus_tag="OO566_004344"
     CDS             complement(318..>591)
                     /locus_tag="OO566_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607335.1"
                     /translation="YLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYK
                     TDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
BASE COUNT          157 a          158 c          143 g          133 t
ORIGIN      
        1 cgggtatccg atttgccgtt gtaaaacggt gcctgattaa acggacaggg atcatccatt
       61 accggggtgt ccatcctgga ctcgctgaag cccaggtaat taaaatcgtc ctttaccgtc
      121 agccggcgac tgacacccac aatctttccc cagacatcaa gaccatactt ttctgcggta
      181 tcgatgttcc agataagacc ataaaaatca ctgataaaac tgtcggggaa agcgctgcgt
      241 taaagctgtt aatgagggca ttgagtcggg ggctggcagc atactgcgca agcacagtgg
      301 cagccacatt ctgcacgtta tgcctcctgg agtttcacac tgatatccga cacatccaga
      361 accggaatct catctatccc gaaagtgaca gccgttgtcc atgacgaacc gtcacggctc
      421 acagtaaggc ccagaatatc aatatgttcc ggatcggttt tgtaaatgcc ggcatagtag
      481 cgcccggcag agacaacaga ggcaactctt gcccgcagac caccatctgt accattaaat
      541 gcgtccagca cggattgctg tacctgtcgg gtaatatctg aaggcaggta g
//
LOCUS       DAJTXY010000330          590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTXY010000330 DAJTXY010000000
VERSION     DAJTXY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.336"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            23..>590
                     /locus_tag="OO566_004345"
     CDS             23..>590
                     /locus_tag="OO566_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6607336.1"
                     /translation="MFTANQCWTSFLDAGGLRDIEVEAVTKMLACGTRILGVKEYICD
                     KPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTLWPVF
                     ESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLN
                     KHGQWKKLSFLKDAMRSRWMWNMRQRLLK"
BASE COUNT          124 a          166 c          178 g          122 t
ORIGIN      
        1 tactccccgc ccgctgaaac gcctgttcac agccaaccag tgctggacgt ccttcctgga
       61 tgcgggcggt ctgcgcgata tcgaggttga ggctgtcacc aaaatgctgg cctgcggcac
      121 gcggatactg ggcgtgaagg aatacatctg cgataaacct gagtgccccc acgtcagata
      181 cgtcactaac tcatgcggca gccgtgcctg cccgtcctgc ggaaaaaagg ccacagacct
      241 gtggatagcg acacagctga atcgtcttcc tgactgcgac tgggtacacc tggtcttcac
      301 cctgccggac acgctgtggc cggtgttcga aagcaaccgg tggctgctga atgacgtgtg
      361 ccgtctggcg gtggagaatc tgctgtatgc cgcccggaaa cgggggcagg aacccggtat
      421 cttctgcgcc atccacacgt atggccgtcg tctcaactgg catccgcatg tacatgtgtc
      481 tgtaacctgt ggaggtctga ataagcatgg tcagtggaaa aagctgagct tcctgaaaga
      541 cgcgatgcgt tcgcggtgga tgtggaatat gcggcaacgg cttctgaaag
//
LOCUS       DAJTXY010000331          545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTXY010000331 DAJTXY010000000
VERSION     DAJTXY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.337"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(7..>545)
                     /gene="ospZ"
                     /locus_tag="OO566_004346"
     CDS             complement(7..>545)
                     /gene="ospZ"
                     /locus_tag="OO566_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR6607337.1"
                     /translation="SMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASTPE
                     GSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFSKENSDFLYIVVVFRNDS
                     PQGELRANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSY
                     IKQMNDRLSQIKARMPVT"
BASE COUNT          164 a          115 c           80 g          186 t
ORIGIN      
        1 tatacattaa gtaacaggca ttcgagcctt gatttgagaa agcctgtcat tcatttgttt
       61 tatgtagcta tctatttcct ctgatgcata actaaaaagt tctcccattt cacgagcaat
      121 aataatctca gatttaatag actttaactc tggcgactca tctcttaaca cttgcataat
      181 ctctcttttt atatcatata gttctataaa ccgatttgcc cgtagttctc cttggggaga
      241 atcatttctg aatacaacaa ctatatacaa aaaatcacta ttttctttag agaagatctt
      301 acaactaatc tctgggagct cacataatgc atgtaagtca ggatggtaca aactatccac
      361 agaaaattgc cccataggac gttttccaga tataactgtc aaccagcttc cttccggtgt
      421 actggcttcg tctctggaaa taggctgaaa atcttccgga catggagaat acattgtctt
      481 catataatta agtcgtctgg atttctcatt aataaactcc tctattttta attgtgccat
      541 acttc
//
LOCUS       DAJTXY010000332          540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTXY010000332 DAJTXY010000000
VERSION     DAJTXY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.338"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            12..>540
                     /gene="fimC"
                     /locus_tag="OO566_004347"
     CDS             12..>540
                     /gene="fimC"
                     /locus_tag="OO566_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6607338.1"
                     /translation="MTFCLLAGILMFMAMMVAGRAEAGVALGATRVIYPAGQKQVQLA
                     VTNNDENSTYLIQSWEENADGVKDGRFIVTPPLFAMKGKKENTLRILDATNNQLPQDR
                     ESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPPDQAAEKLRFRRSA
                     NSLTLINPTPYYLTVT"
BASE COUNT          165 a          115 c          127 g          133 t
ORIGIN      
        1 aatcgcagga aataacattc tgcttgctgg caggtatcct gatgttcatg gcaatgatgg
       61 ttgccggacg cgctgaagca ggagtggcct taggtgcgac tcgcgtaatt tatccggcag
      121 ggcaaaaaca agtgcaactt gccgtgacaa ataatgatga aaatagtacc tatttaattc
      181 aatcatggga ggaaaatgcc gatggtgtaa aggatggtcg ttttatcgtg acgcctcctc
      241 tgtttgcgat gaagggaaaa aaagagaata ccttacgtat tcttgatgca acaaataacc
      301 aattgccaca ggaccgggaa agtttattct ggatgaacgt taaagcgatt ccgtcaatgg
      361 ataaatcaaa attgactgag aatacgctac agctcgcaat tatcagccgc attaaactgt
      421 actatcgccc ggctaaatta gcgttgccac ccgatcaggc cgcagaaaaa ttaagatttc
      481 gtcgtagcgc gaattctctg acgctgatta acccgacacc ctattacctg acggtaacag
//
LOCUS       DAJTXY010000333          536 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTXY010000333 DAJTXY010000000
VERSION     DAJTXY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.339"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            83..463
                     /locus_tag="OO566_004348"
     CDS             83..463
                     /locus_tag="OO566_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR6607339.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
BASE COUNT          164 a           87 c          100 g          185 t
ORIGIN      
        1 tttttgattt gttatttttc cactttcagt atgctaatgc ttgcacctac tgattcatca
       61 aaacaatagt tatggagtat ttatgagtaa ggaatatatg aacgatgatt cattaagtga
      121 aaaatggaaa taccgattta atttttatga tcaacatggc tttcctggat tctggggagc
      181 aacaccagaa tataaagcag catttaaagc attaaaagta agacaacgat taactattca
      241 aatgaatttc attgctttct tctgttcctg gatctattta tttgttctgg gcttatggaa
      301 aaaagcaatt atagttctat tacttggcat tctatctcta tttgttggtg cattaattgg
      361 cgttaacatt ctgggaattg ccgtagcagc ctatgttgct gttaacacga acaaatggtt
      421 ttatgagaaa gaggtaaaag ggttaaatac ctggagcctt tgaataacag gtggcttagc
      481 caggctaagc caccgccttc tttttacaac cagttcttcc gcttaaagta cagata
//
LOCUS       DAJTXY010000334          522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTXY010000334 DAJTXY010000000
VERSION     DAJTXY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.340"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
BASE COUNT          119 a          128 c          143 g          132 t
ORIGIN      
        1 tacccgatta gttgttaatc atatttaatc atagtgatta ctttcagacg gaacaaggca
       61 aataatgcga gtcagcgcat ataaggcggc atacggtggt tacatgtagc acaggtgtaa
      121 aaccgccgaa gcccggcgca agccggtacg gtcgtccgaa tatcaccagt ccccctccgg
      181 ctctgcgagg ccatgcggcc tgtcagcgat gaaaatgcct ctgtgcgcgg tcggagtggt
      241 cccgacaagg gtttacccgc agtcggggcg tatctccgcg ttagcgggcc gtgagggccg
      301 cttacgagcg tgtatgagag acgtacagta gttttgtccg ctcacaccag tacaaaccag
      361 tcgaaatacc ggtaacgacg attactcttg tctctgatgc cggtggacta cgagatttta
      421 tttactggcg tcctgatgcg gtcaggactg gtatggaacc tgtttatgtg atacttaatg
      481 ctcttctgga ttccgggcga tttacgcgta aacttattct gt
//
LOCUS       DAJTXY010000335          515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTXY010000335 DAJTXY010000000
VERSION     DAJTXY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.341"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..359)
                     /locus_tag="OO566_004349"
     CDS             complement(<1..359)
                     /locus_tag="OO566_004349"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607340.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGISADG
                     MYCPECGTVHWPDGVIPP"
BASE COUNT          137 a          126 c          121 g          131 t
ORIGIN      
        1 ggcggaataa cgccatccgg ccagtgaacc gtaccgcatt cagggcaata catgccgtcg
       61 gcgctgatac cggcaggaat tgtcgcaaat tcccgctccg tgcagcgggc aatttccgga
      121 tacccttcgt catcaacacg tacaaaccag aagaccagtt ttttgtttcc ggcatccaca
      181 aagaacggaa tattcaggtc tgcgcaacac tcaacggcat cgtcaaaatt atcaaagcgc
      241 agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt gaagttttac gacatagcgg
      301 aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg gtttagctga acgggccata
      361 taactacctg aaagacaatg atattgtctg tttttataac ggtaatcaca ggccatgaca
      421 agccgcagcc gtcaggccgc ctactcgggg cggtttagaa cgcttattta gtaccagatc
      481 ttacttataa taaaggggct tggcagtttg gataa
//
LOCUS       DAJTXY010000336          513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTXY010000336 DAJTXY010000000
VERSION     DAJTXY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.342"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..502
                     /locus_tag="OO566_004350"
     CDS             <1..502
                     /locus_tag="OO566_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6607341.1"
                     /translation="PTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQS
                     LYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVG
                     RKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPW
                     NFSSVK"
BASE COUNT          149 a          109 c          153 g          102 t
ORIGIN      
        1 tccgacagaa atgactcagg aagcgctcag acggatagca gagttatacg acatagaagc
       61 ggagatacgt ggcagtccgg cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca
      121 gttgatgcag tcgttgtacg actggataca gttgcagagg aaaacgctgt cgaaacatgc
      181 ggagatggcg aaggcgttcg actatatcct gaatcactgg aatgcgctga acgagttctg
      241 tcgtgacggc tgggtggaaa tagacaacaa catcggtgaa aacgcgttac gatcggtggc
      301 ggttggaaga aaaaattatc tctttttcgg ctcagacaag ggaggagaaa gtgcggcgat
      361 catctacagt ctgctggtca cctgcaaaca gaacgaagtg gagccggagg actggttgcg
      421 cgaagtgatc gagaagctca atgactggcc gtcgaaccaa gtgcatgaac tgctgccctg
      481 gaacttctcg tctgtaaaat aatccttacg cta
//
LOCUS       DAJTXY010000337          512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTXY010000337 DAJTXY010000000
VERSION     DAJTXY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.343"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>512
                     /locus_tag="OO566_004351"
     CDS             <1..>512
                     /locus_tag="OO566_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6607342.1"
                     /translation="YLEPETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQ
                     NWKPATLSENRASHSTLIEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSM
                     PLSDLLNRESKTDCLDVVTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKA
                     SDARDAFSPE"
BASE COUNT          133 a          138 c          127 g          114 t
ORIGIN      
        1 taccttgagc ctgagactgg cagtctaaga ccgtccgtct ccctatctgt attggcggaa
       61 cctgaagtcc ctgaacctaa agcactgacc ctcgcgtctc tcattgagca gtacgagcag
      121 gagaacgccc agaactggaa gccagcgacc ctaagcgaaa accgagcgtc acactctacg
      181 ctaatcgaga tattcgacta tctggatatt caggatgtcg gcaaggcgac ccgtgcagat
      241 atgctcagag ttcgtgaagt cctccagcag ctaccgaaga accgcaagca acgctttaag
      301 tctatgccgc tgtctgactt gctgaaccgg gagtctaaga ctgactgtct ggacgtcgtt
      361 accattaaca ataaatatct tatcaagatg gctgctgtgt ttaagtgggc ggtacgcaac
      421 gacttgatag ctaagaacct gaccgaaggt cttgagctga aagtccctca gcgtaaagcc
      481 tccgacgctc gtgatgcgtt cagtcctgag ca
//
LOCUS       DAJTXY010000338          508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTXY010000338 DAJTXY010000000
VERSION     DAJTXY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.344"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>508
                     /gene="ydfJ"
                     /locus_tag="OO566_004352"
     CDS             <1..>508
                     /gene="ydfJ"
                     /locus_tag="OO566_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR6607343.1"
                     /translation="EELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQP
                     TAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDALMI
                     SSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALI
                     VIHNCAVLG"
BASE COUNT          117 a          110 c          131 g          150 t
ORIGIN      
        1 aagaggaact gctggcgtgg ggatggcgta taccgttcct ggcgagcgtt gtcgtgatgg
       61 tctttgctat ctggttgcgt atgaatctga aagaaagccc ggtctttgag aaggttaatg
      121 acagcaacca accgacagca aaacctgcac ctgctggtag catgttccag agcaaatcct
      181 tctggctggc aacagggctg cgttttggtc aggcgggtaa ctccggttta attcagactt
      241 tccttgcagg ctatttagta cagacgttat tgtttaacaa agcaattcca acagatgcat
      301 tgatgatcag ttcgattctc ggctttatga ccattccgtt ccttggttgg ttatccgata
      361 aaattggtcg ccggatcccg tatattatta tgaatacctc tgcgattgtg ctggcgtggc
      421 caatgctttc tatcatcgta gataaaagct atgccccgag caccattatg gttgcactga
      481 ttgtgattca taactgtgcg gtgctggg
//
LOCUS       DAJTXY010000339          506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTXY010000339 DAJTXY010000000
VERSION     DAJTXY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.345"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
BASE COUNT          170 a           95 c          112 g          129 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catattaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggg
//
LOCUS       DAJTXY010000340          503 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTXY010000340 DAJTXY010000000
VERSION     DAJTXY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.346"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..503
                     /locus_tag="OO566_004353"
                     /pseudo
     CDS             1..503
                     /locus_tag="OO566_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          146 a          120 c          136 g          101 t
ORIGIN      
        1 agctcgcctt gagcgagata tattaaaaaa agcaacagcg tattttgcac aggagtcgct
       61 gaaaaatacg cgttaatcga acaatggcga caacaatttc ccattgaagc gatgtgtcag
      121 gtatttggtg tatccaggag cggttattac aactgggtac agcatgaacc ctcagacaga
      181 aaacaaagtg atgagcggct aaaactggag attaaggtgg cacatatccg cactcgcgaa
      241 acatatggaa cccggcggct ccagacggag ctggcagaga atggcatcat cgttggtcgt
      301 gaccgactgg cacgtcttcg taaggagctg aggctacgct gtaagcagaa acgcaagttc
      361 agagcgacta cgaacccgaa ccacaatctg ccagttgcgc caaatctgct gaaccagacg
      421 ttcgctccta cagcaccaaa tcaggtctgg gtggcggacc tgacgtatgt tgccacacag
      481 gagggatggt tgtacctcgc tgg
//
LOCUS       DAJTXY010000341          496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTXY010000341 DAJTXY010000000
VERSION     DAJTXY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.347"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..258
                     /locus_tag="OO566_004354"
     CDS             <1..258
                     /locus_tag="OO566_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607344.1"
                     /translation="YNGEDRGVFMMAKRFTALSFGCFRHDGESITVVLSRDGDSQSHD
                     LEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          124 a          111 c          127 g          134 t
ORIGIN      
        1 tacaacggtg aagaccgtgg cgtgttcatg atggctaagc gttttacggc tctgagcttc
       61 ggttgcttcc gtcacgatgg cgagagcatt actgtggtcc tttcacgtga tggagactcc
      121 cagtctcacg accttgagat tctcggcaag gtgtttgttg tagctgaagc tgctacgacc
      181 atcaagatgg gtactggctc ggctactgct gtcctgcctg tagtcaaacg cctgagcttc
      241 actaaagaag ctaactaaca ataacgacta ttggtcagac ttccgaactg gatgtctggc
      301 ctttattttg tactagtaat ctagttcgtg aatacagaaa gtaggagggc caaatggacc
      361 tacacaaagt ctatctcgtg gtcttcctgc taaagctggg actggtggtc tggctatggg
      421 ctattagacg atgagttttg actgaaaaat gtgagacctc atctaatata aagagcgagc
      481 caaaagcccc catagc
//
LOCUS       DAJTXY010000342          494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTXY010000342 DAJTXY010000000
VERSION     DAJTXY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.348"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>494
                     /locus_tag="OO566_004355"
     CDS             <1..>494
                     /locus_tag="OO566_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6607345.1"
                     /translation="ASNRIRGLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLAS
                     LGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRK
                     QRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLA
                     PVTR"
BASE COUNT           94 a          170 c          133 g           97 t
ORIGIN      
        1 ggccagcaac cgtatccgcg gccttctgac ccagatacat ccggcactgg agcgcgttct
       61 cggtccgaga cttgagcacc cggcggtact cgatcttctc cagcgatatc cctcaccaga
      121 aaaactcgct tcgctgggtg agaagaagct ggcagcccag ctctgcaaac ttgcgcctcg
      181 tctgggtaaa cgccttgcag cagacatagc tcaggcactg gccgaacaaa ccgtcgtcgt
      241 tcccggcacg aatgccgctg ccgtagtact gccacgtctg gcactccagc tcatcacgct
      301 gcgtaagcaa agagacgagg tggcgcttga ggtagaacag cgagttcttg ctcaccctct
      361 ttacccggtc ctgaccagta tgcccggagt cggtgtcagg accgcagcca gactcctcac
      421 cgaggtcgcc tgccgcgcct tcgcctctgc cgcacatctc gctgcttatg ctggccttgc
      481 gccggtaact cggc
//
LOCUS       DAJTXY010000343          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTXY010000343 DAJTXY010000000
VERSION     DAJTXY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.349"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..240)
                     /locus_tag="OO566_004356"
     CDS             complement(<1..240)
                     /locus_tag="OO566_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6607346.1"
                     /translation="MHEKNITLLCDEADRLLQLSINLLRQMVDEPDVLSDGKNENGQL
                     FDKQKALKRIKELEGEQIKTARREMVLAVVGTMKAG"
BASE COUNT          129 a          124 c          100 g          136 t
ORIGIN      
        1 gcctgctttc atcgtgccga caacagccag caccatctcc ctgcgggcgg ttttgatttg
       61 ttcgccctcc agctccttaa ttcttttcag tgctttctgt ttatcaaaaa gctgcccgtt
      121 ttcgttctta ccgtcagata acacatccgg ctcatcaacc atttgccgga gcagattgat
      181 gctcagttgc aaaagccggt cggcttcatc acaaagcagg gtgatgttct tttcgtgcat
      241 tatttaacct tcctgagacg cattttcatg acaaccacgg aggaggccat ttactacaag
      301 cgatcgctta taacgatcaa tataatagaa atgatcgtca tattctatta atatgaaaaa
      361 cacaacgcaa gggggacaac ccgggtatgg ctgcgtttgc attgtgtttt catagagtta
      421 aaaatctgta acgacaaccc ggcacatcac taccgggtta agaagaaaat ccagttccat
      481 accgcgcgg
//
LOCUS       DAJTXY010000344          485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTXY010000344 DAJTXY010000000
VERSION     DAJTXY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.350"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..421
                     /gene="hydN"
                     /locus_tag="OO566_004357"
     CDS             <1..421
                     /gene="hydN"
                     /locus_tag="OO566_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6607347.1"
                     /translation="PETFLPRIHVIKGVNISTATVCRQCEDAPCANVCPNGAISCDKG
                     FVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHRE
                     DGPACMAACPTHALICVDRNKLEQLSAEKRRRTALMF"
BASE COUNT          119 a          113 c          139 g          114 t
ORIGIN      
        1 cccggaaaca tttttaccgc gtatccatgt cattaaaggt gtgaacattt ccacggcgac
       61 agtttgccgt cagtgtgaag atgcaccgtg cgctaacgtc tgcccgaatg gtgctatcag
      121 ctgtgataaa gggtttgttc atgtgatgca ggaacgttgc attggttgca aaacctgcgt
      181 ggtggcttgc ccgtatggcg cgatggaagt ggtggtacgt cctgtgattc gcaacagcgg
      241 cgcgggtctg aatgtacggg ctgacaaagc cgaagccaat aaatgcgacc tgtgcaacca
      301 tcgtgaagac ggcccggcgt gtatggcggc ttgcccgacc catgcgctga tttgtgtcga
      361 tcgtaataaa cttgaacaac tgagcgcaga aaaacgccgc cgtacggcgc tgatgttcta
      421 aaaatcttac agaactgttc tggcatcaaa ggaattcccg gaaatattcg tagaatacgg
      481 agcgg
//
LOCUS       DAJTXY010000345          479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTXY010000345 DAJTXY010000000
VERSION     DAJTXY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.351"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..453
                     /locus_tag="OO566_004358"
     CDS             <1..453
                     /locus_tag="OO566_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6607348.1"
                     /translation="RMWGRWSYIGGGRSGNMFNQLLASQKMTKTAVNEALRRLKKAGI
                     DKPELEAFLREMIEGKQKSWLTHCTDAEALKIDSVIGEVLADHPGLLNVLSQRYVGRG
                     MSKRRMAELLNEQYPEWALITCRRRVDQWLRVAEFILYSPMREAFDYA"
BASE COUNT          145 a           83 c          137 g          114 t
ORIGIN      
        1 cgcatgtggg ggcgctggtc gtatattggc ggcggcaggt cagggaatat gtttaaccag
       61 ttgctggcat cccaaaaaat gacaaaaaca gccgtcaatg aagccctgcg cagattgaaa
      121 aaagcgggaa ttgataaacc agagttggaa gcatttttgc gtgagatgat cgaaggaaag
      181 caaaaaagct ggttaactca ctgcactgac gcagaggcac taaaaattga tagtgttata
      241 ggtgaagttc tggcggatca tccaggacta ctaaatgtcc tgagtcagcg ttatgtgggg
      301 cgagggatga gtaagagaag gatggccgag ttattaaacg aacagtaccc agagtgggcg
      361 ttgattacat gccgacgtcg tgttgatcag tggttgcgtg ttgctgagtt cattttgtat
      421 tcaccgatga gagaagcgtt cgattatgct taaaaaatca ttgcaaaatg agccacaaa
//
LOCUS       DAJTXY010000346          478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTXY010000346 DAJTXY010000000
VERSION     DAJTXY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.352"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..171
                     /locus_tag="OO566_004359"
     CDS             <1..171
                     /locus_tag="OO566_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6607349.1"
                     /translation="CSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGA
                     VFGGELTLVQLQ"
     gene            complement(172..363)
                     /locus_tag="OO566_004360"
     CDS             complement(172..363)
                     /locus_tag="OO566_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6607350.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
BASE COUNT          136 a          117 c          111 g          114 t
ORIGIN      
        1 tgtagccgct cgcgattagg tgaacgccca aaagggcagg gaattacagg tgctgatcat
       61 ttctggtttg gtcatacacc gttgcgacat cgcgtggata ttggcaacct gcattatatt
      121 gataccggtg ctgtctttgg gggcgaactg actcttgtgc aactgcaata attaaaaatc
      181 accatactcc tgtgctggtc gccagaaacc atctataaaa tcctcaatcg gaaaacaacc
      241 gccatggcgg atccgttgat cgctcataga ataaagacac tgctgttccg tgttgtagac
      301 atccacaaca atatcttcac aaccgccatc caggtagcaa acaaaaagta ccagcgcgaa
      361 catttcatcc ccgaagtgtg gtgccgtacc gttaagttta ggagagattt tacaacgggg
      421 gaataaccag gacaaataac ccgccatgcc gacgggttct ttttggatca ggcaagac
//
LOCUS       DAJTXY010000347          475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTXY010000347 DAJTXY010000000
VERSION     DAJTXY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.353"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..87)
                     /locus_tag="OO566_004361"
     CDS             complement(<1..87)
                     /locus_tag="OO566_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6607351.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINR"
     gene            complement(74..445)
                     /locus_tag="OO566_004362"
     CDS             complement(74..445)
                     /locus_tag="OO566_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6607352.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMISRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
BASE COUNT          129 a          140 c           97 g          109 t
ORIGIN      
        1 acggtttatc gcccatgcgc tggcctgtgg caccatacgg gtatcaaggc tgttcagatt
       61 gcggatggca ttctcaagcc ccttcataat atcgtcctgc ccttacgttt cccggcagcc
      121 cctcacgccg caaccggata taactacatc cgccatcatc aggggtgata cgatccacac
      181 agaaagactc acgactaatc atcagcgaat cattgcgaca cagccgtaaa atatctgctg
      241 ttttcacaaa cagggatggt aaagagtctt caatacgcac accgccggcc accagagaaa
      301 tattttcagg atcatcaaaa accccggaaa tcatgatccc ttttagccgg cctgacgtaa
      361 tgaccgctct aatccccata ttgcgaataa tggccttgtc tgcgagatca agtgcaacat
      421 caaacagatt atcgaaatca gccacgctgc ctcccgttat tgcattctgg ccagg
//
LOCUS       DAJTXY010000348          457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTXY010000348 DAJTXY010000000
VERSION     DAJTXY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.354"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>457
                     /locus_tag="OO566_004363"
     CDS             <1..>457
                     /locus_tag="OO566_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6607353.1"
                     /translation="GGHEHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVV
                     LDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLL
                     WRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT          107 a          110 c          146 g           94 t
ORIGIN      
        1 gtggtggtca tgaacatgtt gctatcccta actaccttga aaggcagttc gccgtgaccg
       61 agccaaatca ggtgtggtgc ggtgatgtga cctatatctg gacgggtaag cgctgggcgt
      121 acctcgccgt tgttctcgac ctgttcgcaa gaaaaccagt gggctgggcc atgtcgttct
      181 cgccggacag caggctcacc atgaaagcgc tggaaatggc atgggaaacc cgtggtaagc
      241 ccggcggggt gatgttccac agcgatcagg gcagtcatta tacgagcagg cagttccggc
      301 agttattgtg gcgataccag atcagacaga gtatgagccg gcgcggaaac tgctgggata
      361 acagcccaat ggaacgcttc ttcaggagtc tgaagaacga atggatgccg gtggtgggtt
      421 acgtaagctt cagcgaggca gctcacgcca taacgga
//
LOCUS       DAJTXY010000349          452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTXY010000349 DAJTXY010000000
VERSION     DAJTXY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.355"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..92)
                     /locus_tag="OO566_004364"
     CDS             complement(<1..92)
                     /locus_tag="OO566_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HCR6607354.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            148..300
                     /locus_tag="OO566_004365"
                     /pseudo
     CDS             148..300
                     /locus_tag="OO566_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          133 a           89 c           89 g          141 t
ORIGIN      
        1 tcaactctag gataaatatt tctcactata tattcagtat ttgcagtatt tattggaaat
       61 acgttcttaa tattcttgat gggactaatc attttaatct ctatactcaa atataaacat
      121 taccatgaac aatataattg ttcctaagtg gaatgtctcc acggagaaca ctttatctat
      181 cgggaaatag tgcgggcaac agtgtttaat tatatcgagt gcgattacaa tcgctggcga
      241 cggcaccgtt ggtgtggcgg cctcagtccg gaacaatttg aaaaccagaa cctcgcttag
      301 gcctgtgtcc atattacgtg ggtaggctca cagagcctca cttacgactg cgtccatatt
      361 atgtgggtag gatcaataaa gacgttttat atgtgtctgc ttgcagccag aaaatttata
      421 ccctgcaggt atcataaaca tgcttgataa ct
//
LOCUS       DAJTXY010000350          444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTXY010000350 DAJTXY010000000
VERSION     DAJTXY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.356"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(26..235)
                     /locus_tag="OO566_004366"
                     /pseudo
     CDS             complement(26..235)
                     /locus_tag="OO566_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
BASE COUNT          134 a          113 c           82 g          115 t
ORIGIN      
        1 tatcgcagct ctgacgactc actccttatt tacccgggaa atacttctcc agcaatgcat
       61 acaggatggc agcggtttca gcatccatta tgccgtcata atttttctgc cggaaatgaa
      121 gctgaaaagc gcggacaagc gccttatatc cgtcatccgt ctccacacca gaaatactgt
      181 atccataggt cttcagccgt gacacgatat caaccttatc cggaatgccc tgcgattcaa
      241 aaaataaata tttagtcagt aattttatgt ttttcctgag aaaattactc tttttcgaac
      301 tccagtgagc gttcacgact catcagtgtg aaaatgatgg caaaaacagc agacagtatc
      361 agtacgataa atgtggcccc ccatcccaga taatcgacaa caagtcccat tcccagtgtc
      421 gcaacaattg agccgaagat atag
//
LOCUS       DAJTXY010000351          444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTXY010000351 DAJTXY010000000
VERSION     DAJTXY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.357"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>444
                     /locus_tag="OO566_004367"
     CDS             <1..>444
                     /locus_tag="OO566_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6607355.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGR"
BASE COUNT          113 a          105 c          133 g           93 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgg
//
LOCUS       DAJTXY010000352          440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTXY010000352 DAJTXY010000000
VERSION     DAJTXY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.358"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>440
                     /locus_tag="OO566_004368"
     CDS             <1..>440
                     /locus_tag="OO566_004368"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6607356.1"
                     /translation="VNIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYV
                     LAHAYLRDDHVLDAVLPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVT
                     TDDRNWELRYSASALRFNLSRAVAIDMESATIAAQGYRFRVSYG"
BASE COUNT          100 a          104 c          123 g          113 t
ORIGIN      
        1 tggtgaatat tggcgtggga ccgtccaatg ctaaaaccat ctgcgatcat ctggcagtgc
       61 tacgcccgga tgtctggttg atgattggtc actgtggcgg attacgtgaa agccaggcca
      121 ttggagatta tgtccttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc
      181 tgccgcccga tatacctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcta
      241 ccaagttagt cagcggcagg cctggtgagg aagtcaaaca gcggctgcgt acaggtactg
      301 tggtaaccac agatgacagg aactgggagt tacgttactc agcttctgca cttcgtttta
      361 acttaagccg ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc
      421 gtttccgcgt gtcatacggg
//
LOCUS       DAJTXY010000353          430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTXY010000353 DAJTXY010000000
VERSION     DAJTXY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.359"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..108)
                     /locus_tag="OO566_004369"
     CDS             complement(<1..108)
                     /locus_tag="OO566_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6607357.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVA"
     gene            202..381
                     /locus_tag="OO566_004370"
                     /pseudo
     CDS             202..381
                     /locus_tag="OO566_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          125 a           99 c           87 g          119 t
ORIGIN      
        1 ggcaacaagg gagaccgtca ttcccggttc aaaactctgc tgaacaatag cgatcttttc
       61 ctgtgtagta cgccgtctgc gtttctccgg tcctaagaca tcaatcatct gctctccaat
      121 gactagtcta aaaactagta ttaagactat cacttaaata agtgatattg gttgtctgga
      181 gattcagggg gccagtctac aggttacaaa gattctgatc gtctgaacta tattgaaatc
      241 ggtacctggt attacttcaa taagaatatg aacatatata ccgcttatca gatcaacctg
      301 cttgataaga gtgattatgt tctcgctcat ggactgaaca ccgacgacca actggcagtt
      361 ggtatcgtct accagttcta atcagccaac gcgccgaggc gtgttactgt aaaacataac
      421 accagcctga
//
LOCUS       DAJTXY010000354          429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTXY010000354 DAJTXY010000000
VERSION     DAJTXY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.360"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..279
                     /locus_tag="OO566_004371"
     CDS             <1..279
                     /locus_tag="OO566_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6607358.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          109 a           99 c          115 g          106 t
ORIGIN      
        1 agcctgaacg tgtactgtcg taatggccag gtggaaatcg acaacaacat cggtgaaaac
       61 gcgctgcgat cggtggcggt tggaagaaaa aattatctct tcttcggttc tgacaacggt
      121 ggagaaagcg cggcgattat ctacagtctg ctggtcacct gcaaactgaa cggagtggag
      181 ccggaggact ggttgcgcga agtgatcgtg aagctcaatg actggccgtc gaaccgagtg
      241 catgaactgc tgccctggaa cttctcagct gtaaaataat ccttccgcta cgtacttctc
      301 tgggcgctta ccactcaacg gcatcgtcaa aattatcaaa gcgcagaaca tagatttatc
      361 atgtgtttta tggatggctt tctccatcag gcatcgttcg tcacgggaaa ttggtgctat
      421 gatcggcat
//
LOCUS       DAJTXY010000355          423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTXY010000355 DAJTXY010000000
VERSION     DAJTXY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.361"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>423
                     /locus_tag="OO566_004372"
     CDS             <1..>423
                     /locus_tag="OO566_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR6607359.1"
                     /translation="LLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPL
                     YVSLYPTEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFR
                     PREVQGTMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIV"
BASE COUNT           91 a          105 c          122 g          105 t
ORIGIN      
        1 cttcttcgcc atatatcgat aatcaacggc ttcgacaatc cgatgactca gccgttgttg
       61 tcagatgggc ccctgacagc gttgatggat cattatcttg atacagatgc tctggcaggc
      121 gggctaccgc tgtatgtgtc gctgtacccc acagaagggg gcatgcagga tattattgac
      181 tgcattcgtg ctgaactggg ttccggaacc acgaaaaacg ccgtttttca gcatatccag
      241 agtctgcccc gcggacagca gaaagaggct ctgcttgcgt cagccgcact gcccctgttg
      301 ttccgtcccc gtgaggttca ggggacaatg ttcggtgatg gtggtatggg aggatggcga
      361 aatatgcagg gaaatacccc tgtaacgcct ctggtcgatg ccggatgcaa tatggtgatt
      421 gtg
//
LOCUS       DAJTXY010000356          423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTXY010000356 DAJTXY010000000
VERSION     DAJTXY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.362"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            172..>423
                     /locus_tag="OO566_004373"
     CDS             172..>423
                     /locus_tag="OO566_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607360.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKS"
BASE COUNT          173 a           60 c           69 g          121 t
ORIGIN      
        1 tgcaataatt cctttcagta aaggaagtgt gattaaactc aggggatttc agagtgaaaa
       61 tcgtacaaca tattaaaata tgttcgttat aatacataca agaaaatatt aagttagata
      121 atattattct aaataactgc agataaaaac gcacataatg ggggatgttt tatgaaaata
      181 cctgaagcag taaatcatat taatgttcag aacaatattg atcttgttga tggaaaaata
      241 aatccgaaca aagatacaaa agcattacaa aaaaacatat catgcgtaac aaactcatcc
      301 tcttctggca taagtgaaaa acatttagat cactgtgctg atactgtgaa aagcttttta
      361 aggaagtcta tagcagcgca aagttatagt aaaatgttct ctcaagggac cagtttcaaa
      421 tca
//
LOCUS       DAJTXY010000357          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTXY010000357 DAJTXY010000000
VERSION     DAJTXY010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.363"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>418
                     /locus_tag="OO566_004374"
     CDS             <1..>418
                     /locus_tag="OO566_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6607361.1"
                     /translation="AVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALD
                     CCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETR
                     QFARMLGLEPKSTAVRSPESNGIAESFVKTIKRDYIS"
BASE COUNT          102 a           98 c          140 g           78 t
ORIGIN      
        1 gctgtaccgc catcgaaacg ggcacatacc ggcagagtgg ctgtgaaaga aagtaatcag
       61 cgatggtgct ctgacgggtt cgagttccgc tgtgataacg gagaaaaact gcgagtcacg
      121 ttcgcgctgg actgctgtga ccgtgaggca ctgcactggg cggtcactac gggcggcttc
      181 aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg aacgccgctt cggcaacgag
      241 cttccggcgt ctccagtaga gtggctgacg gataatggtt catgctaccg ggctaatgaa
      301 acacggcagt ttgcccggat gttggggctt gaaccgaaga gcacggcggt gcggagtccg
      361 gagagtaacg gcatagcaga gagcttcgtg aaaacgataa agcgtgacta catcagtg
//
LOCUS       DAJTXY010000358          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTXY010000358 DAJTXY010000000
VERSION     DAJTXY010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.364"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            258..>418
                     /locus_tag="OO566_004375"
     CDS             258..>418
                     /locus_tag="OO566_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6607362.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNS"
BASE COUNT          100 a          103 c          106 g          109 t
ORIGIN      
        1 tctgcgttgt gtgattatat cgcgtaccac ttttcgactg ttttaccgcc ggtattctgc
       61 cgtctgacgc attgacgggt atttctgcct gacaatactg tcactgccaa aaaaactgcc
      121 gtgcctttgt cggtaattcg aacttgctga caggacagga tgtgtaattg ttataccgcg
      181 catacatgca cgctattaca attaccctgg tcagggcttc gccccgacac cccgtgtcag
      241 atacggagcc atgttttatg acaaaacgaa gtggaagtaa tacgcgcagg cgggctatca
      301 gtcgccctgt tcgtctgacg gcagaagaag accaggaaat cagaaaaagg gctgctgaat
      361 gcggcaagac cgtttctggt tttttacggg cggcagctct cggtaagaac gttaactc
//
LOCUS       DAJTXY010000359          414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTXY010000359 DAJTXY010000000
VERSION     DAJTXY010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.365"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(201..>414)
                     /locus_tag="OO566_004376"
     CDS             complement(201..>414)
                     /locus_tag="OO566_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR6607363.1"
                     /translation="NSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRS
                     ETVLQKSLEITEEADLALRSAMARIK"
BASE COUNT          104 a          113 c           84 g          113 t
ORIGIN      
        1 aacaccatca tacactaaat cagtaagttg gcagcatcac ctaagtgctt ttacgatgaa
       61 tttcatgata actctcctta tgacattaat aaatgcgtta atcttaaatc tcacccaggt
      121 aatatatatc tgaaaatatt ttcaacaaca ccagacagga aaaacaccac cacaggcggt
      181 ggcgtttctt tggggcgtct ttatttaatc cgggccatcg cgcttctcag tgccaaatct
      241 gcctcttccg ttatctccag acttttctgc aacaccgttt ctgaccggga cagtgcctga
      301 cgtgctgcca gcggtttccg gatatgctcc atcgtcagca tcgtgtcctc gtagccctgc
      361 tgacgccagg cgtcggtatg tgccgacgtg aaactgagca gaccgccact gttg
//
LOCUS       DAJTXY010000360          412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTXY010000360 DAJTXY010000000
VERSION     DAJTXY010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.366"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..236
                     /locus_tag="OO566_004377"
     CDS             <1..236
                     /locus_tag="OO566_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607364.1"
                     /translation="IEQDINQPVRQEIDAFFCAFREKVEQLRNTLIQSSEDHKRDQQA
                     QERLTRRLQALNERVPELITDSKALREELETML"
     gene            233..>412
                     /locus_tag="OO566_004378"
     CDS             233..>412
                     /locus_tag="OO566_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607365.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEG"
BASE COUNT          115 a           96 c          109 g           92 t
ORIGIN      
        1 ccattgagca ggatattaac cagcccgtcc gccaggaaat cgatgccttt ttctgcgcat
       61 tcagggagaa agttgaacaa ctgcgtaaca cgctgattca gagctctgaa gatcataaac
      121 gcgatcagca ggcacaggaa cggcttacca ggcgacttca ggcattaaat gaaagggttc
      181 ctgagctcat tactgacagt aaggcgctga gggaagagct ggagacaatg ctgtgacttc
      241 accatttatt caacaaattg ctgataaccg ggtatgtcag gtactcacct gtcttcctga
      301 aaaatttgtg gttgatttcg caaacggaat cgatgttgca caggagcata tccgcacggc
      361 cggagagcgc acgttcttcc ggcgtttaaa agagggactg accggcgaag gc
//
LOCUS       DAJTXY010000361          411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTXY010000361 DAJTXY010000000
VERSION     DAJTXY010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.367"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(68..298)
                     /locus_tag="OO566_004379"
     CDS             complement(68..298)
                     /locus_tag="OO566_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCR6607366.1"
                     /translation="MLTIRVTDDEHVRLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGDELRRLRLAVREQGTRDDS"
     gene            266..>411
                     /locus_tag="OO566_004380"
     CDS             266..>411
                     /locus_tag="OO566_004380"
                     /inference="COORDINATES: protein motif:HMM:NF013937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6607367.1"
                     /translation="MFIISNPYREHPLAFHRYHYLMNRNPPYTEVSVTKQEKTALNMA
                     RFIR"
BASE COUNT           95 a          145 c           90 g           81 t
ORIGIN      
        1 gttacgctcc cttcccagca ggtaatcaac cggaccactg ccaccacctt ttccccgggc
       61 atgaaactta actatcatcc cgcgtcccct gttccctgac ggccagacgc agccggcgca
      121 gctcgtcccc gatggcagcc agctgacgca gtaacggcgg tgccagtgtc ggcagtttcc
      181 cggaacgggc aaccggctca cccaggcaga cccgccgcat ccataccgcc agctgtttac
      241 cctcacagcg ttccagtaac cggacatgtt catcatcagt aacccgtatc gtgagcatcc
      301 tctcgcgttt catcggtatc attacctcat gaacagaaat cccccttaca cggaggtatc
      361 agtgactaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa g
//
LOCUS       DAJTXY010000362          409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTXY010000362 DAJTXY010000000
VERSION     DAJTXY010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.368"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..>409)
                     /locus_tag="OO566_004381"
     CDS             complement(<1..>409)
                     /locus_tag="OO566_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6607368.1"
                     /translation="FAAKCGLSDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQS
                     QSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPE
                     IFSSDDSIQADLQGISQQTLQTAKMLESLPTDTR"
BASE COUNT          108 a           85 c           84 g          132 t
ORIGIN      
        1 acgagtatct gttggtaatg attcaagcat tttggctgtt tgtaaggttt gctgcgaaat
       61 accttgcaaa tcagcctgga tggaatcatc ggaagaaaaa atctcaggca taagagcttc
      121 aaatcctttc tttctgaaca actcaatcgc agaatgcagt tccttgtcct ctaacaattc
      181 gacaattgcg atcagtttag ttttcaatgt gctgcggtca ggttgagaat ttttattctc
      241 ttgattattt ttatgctcaa ccattggcat gctctgactt tggataaacc actctattgg
      301 acgccctgtt gcgtaggcta tttgtgctaa tcgatctaaa gacgggtatg tcttgccaga
      361 caggtagttt ctgataaccc catcagaaag tccgcactta gctgcaaac
//
LOCUS       DAJTXY010000363          405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTXY010000363 DAJTXY010000000
VERSION     DAJTXY010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.369"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            34..393
                     /locus_tag="OO566_004382"
     CDS             34..393
                     /locus_tag="OO566_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6607369.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
BASE COUNT          136 a           69 c           95 g          105 t
ORIGIN      
        1 cgtgccatat cgttataatc cctttgatat tagatgcaaa ttaaggtcat atatagcctt
       61 atcgataaca tggttaattt taaggataag aatatgcctg ctgtaataga taaagccctg
      121 gatttcattg gtgccatgga tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg
      181 gcgaagggaa tctttaaata tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt
      241 acggcgcgag ctgaccagga aggctggaac cctgggttca cggaaaaaat ggttggatgg
      301 gcaaaaaaaa tggagacagg tgaacgctct gtgattaaaa atcctgaata cttttcaaca
      361 tatatgcagg aagaactaaa agcactggtc tgagttaaat ttata
//
LOCUS       DAJTXY010000364          401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTXY010000364 DAJTXY010000000
VERSION     DAJTXY010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.370"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>401
                     /locus_tag="OO566_004383"
     CDS             <1..>401
                     /locus_tag="OO566_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6607370.1"
                     /translation="NHLLTSSKNIKTHPSPNMDVTSHRRLFQKDGIGLSQTGKATQLV
                     IMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTKGLSVLSEVNKRHT
                     EAQKGLTPSLNISKDDLKVLANARNIPQSKL"
BASE COUNT          161 a           92 c           67 g           81 t
ORIGIN      
        1 aatcaccttc taacatcatc caaaaatata aaaacacacc catcaccaaa catggacgta
       61 acaagtcatc gtcgtttatt ccagaaagac ggaataggat tgagccaaac aggaaaagca
      121 acacaacttg tcattatgtc gcatggtgga tggaaagaga tctcccatcc acaaacacta
      181 ttcacgcgtc aaaaaggcga tggatggact gtagtcccca aaaacctaag aattgatttt
      241 tatacaaaag acaacgactt cacaaaggga ctaagcgttc tttcagaggt aaacaaacgc
      301 catactgaag ctcaaaaagg gttaacacca tcactaaaca taagcaaaga tgacctaaaa
      361 gttctagcga acgcgcgcaa tatacctcaa tcaaaattag a
//
LOCUS       DAJTXY010000365          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTXY010000365 DAJTXY010000000
VERSION     DAJTXY010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.371"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            108..>388
                     /locus_tag="OO566_004384"
     CDS             108..>388
                     /locus_tag="OO566_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6607371.1"
                     /translation="MEYRTWITEALRLHFEEYLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRRP"
BASE COUNT           88 a          111 c           89 g          100 t
ORIGIN      
        1 aagcgcagaa cctcagcttc ttcgtaaccg gctcatttaa accgtctggt ctgtttcctc
       61 cggctccaca aaaataatgt ccatcatttt gaagtggaca ctatcgtatg gaataccgga
      121 cctggatcac tgaagcttta cgccttcatt tcgaagaata tttaccacgg gttgtagccg
      181 gacgtcgcct gggcgtacca aaatcaactg tctgtggtat gttcgtgcgc ttccgcaacg
      241 ctggcctttc atggcctctg cccgcaggta tgtcggagca ggaacttgat gcccttcttt
      301 acggaagtgc ctccacagta cctgtcgtac ttactgaaag caccgttatg cccaaactcc
      361 cggtggtaaa aaaacgtccc cggcggcc
//
LOCUS       DAJTXY010000366          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTXY010000366 DAJTXY010000000
VERSION     DAJTXY010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.372"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..147
                     /locus_tag="OO566_004385"
     CDS             <1..147
                     /locus_tag="OO566_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCR6607372.1"
                     /translation="DWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQ
                     KGAA"
     gene            144..>387
                     /locus_tag="OO566_004386"
     CDS             144..>387
                     /locus_tag="OO566_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR6607373.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNR"
BASE COUNT           85 a           91 c          125 g           86 t
ORIGIN      
        1 gactggtgca gtgaagagca gcatccggtg atctggtcaa ccacgcaggc tgcagaacac
       61 ttaccggttc gcatgccccg tctctgctct gcagaacgcc tctctgaaag tatggtcgat
      121 gaaatatttc agaagggggc tgcatgagta tagaaataaa aacagggctg gtgctgtccg
      181 gcggtggggc ggtgggcgct tatcaggcgg gagtggttaa ggcactggca gagtgtggta
      241 cacagatcag catggtttca ggggccagca ttggcgcatt caatggtgcc attatcgcgg
      301 cctctcccga tctgtcagaa gctgccgtac gcctggaggc gctctgggat catcttggga
      361 ataatcaggt gctgtcggta aaccgat
//
LOCUS       DAJTXY010000367          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTXY010000367 DAJTXY010000000
VERSION     DAJTXY010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.373"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
BASE COUNT          127 a           93 c           54 g          104 t
ORIGIN      
        1 caccccaaat gcatttcttt ataaatttaa tctgtaacaa aaccgcttcc catatagaaa
       61 tatttgcaaa aatagtgcat gaaaaaagct tttccctacc aatccgggat ataaataatc
      121 atcagtacca caattattcc acagcaataa atctcttccc gccgctccgg acctctgata
      181 atggtctaaa atcattgagg ccacttgcga cgacattgct caatgaggaa agtttggcgt
      241 gaataatatt caacaccagt gcagaatcat cattacatac ttatgcccgc caactaccgc
      301 tttcgatata ttgactcaat ttttcagcct ggttaaaaat agcgaccagc tcagtcgcaa
      361 atatagtgac taccctaa
//
LOCUS       DAJTXY010000368          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTXY010000368 DAJTXY010000000
VERSION     DAJTXY010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.374"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..339
                     /locus_tag="OO566_004387"
     CDS             <1..339
                     /locus_tag="OO566_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="regulates several genes involved in high affinity
                     phosphate uptake; under conditions of high phosphate
                     concentrations downregulates the PHO regulon; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate transport system regulator PhoU"
                     /protein_id="HCR6607374.1"
                     /translation="SLGRHTIQMLHDVLDAFARMDIDEAVRIYREDKKVDQEYEGIVR
                     QLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVKGQDFRHVGSDELD
                     KLLAGKDSDK"
BASE COUNT          102 a           81 c           98 g           97 t
ORIGIN      
        1 tcgctgggcc gtcataccat ccagatgctg cacgacgtgc tggacgcgtt cgcgcggatg
       61 gacattgacg aagcggtacg tatttatcgt gaagataaaa aagtcgatca ggaatacgaa
      121 ggtattgttc gtcaactgat gacctacatg atggaagatt cgcgcactat tccgagcgta
      181 cttaccgcgc tgttctgtgc gcgttcaatc gaacgtatcg gcgaccgctg ccagaatatt
      241 tgtgagttta tcttctacta cgtgaagggt caggatttcc gtcatgtagg cagtgatgag
      301 ctggataaac tgctggcggg aaaagatagc gacaaataat tcaccagaca aatcccaata
      361 acttaattat tgggattt
//
LOCUS       DAJTXY010000369          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTXY010000369 DAJTXY010000000
VERSION     DAJTXY010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.375"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(195..>373)
                     /locus_tag="OO566_004388"
     CDS             complement(195..>373)
                     /locus_tag="OO566_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607375.1"
                     /translation="NVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCSGQAF
                     PADNNGYAPPPCRK"
BASE COUNT           98 a           87 c          103 g           85 t
ORIGIN      
        1 aaaaaaccaa aggcattgga aaccagtgag ttatcgtatt gatgacctaa ggtgctcatg
       61 gatggactcc tattacaaag tcgatataaa aaaacccttc cgcgtcgtta ggcccgacga
      121 ggaagggttg gatttgcgaa acacaagttt gtgctaatta tcgccttaaa tgggcgaggc
      181 atcaagcgag gttgctattt ccgacacggt ggcggcgcat aaccattgtt atctgccgga
      241 aaggcttgtc cagagcacgg cggcagcgcg ttttgcgccg ggccttgtaa ttcaaaggcg
      301 gcaaagccga ttaccccggt attacgcaca tcgccttgcg ccgaattggc tgcgtaggaa
      361 tcagcaacgt tgg
//
LOCUS       DAJTXY010000370          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTXY010000370 DAJTXY010000000
VERSION     DAJTXY010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.376"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(288..>371)
                     /locus_tag="OO566_004389"
     CDS             complement(288..>371)
                     /locus_tag="OO566_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6607376.1"
                     /translation="NAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          103 a           86 c           76 g          106 t
ORIGIN      
        1 ctcaggctac ctcactcccg taaagacagg agtcaagttc ccgctccgac ataactgcgt
       61 gcaatggatt ttgaaggaac cgtcagccca ttccccggag gcaaacatgg gctggcaaaa
      121 gtgtagcggt attaggcaca gctatttagg aaagagggag catctccatc tcattaaatg
      181 gaccacgcca gacaattcac tgtcatgctg atgctgcact gtggggacag aatcactcat
      241 gatgacttcg ataatcgacg acattattat ttggccgagc ttttaggtca tatctcaaat
      301 cgtttgccta atattgctcc attttttaat aaaaaatcaa tcatttttga atctttgctt
      361 ttcattgcat t
//
LOCUS       DAJTXY010000371          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTXY010000371 DAJTXY010000000
VERSION     DAJTXY010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.377"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(80..>371)
                     /locus_tag="OO566_004390"
     CDS             complement(80..>371)
                     /locus_tag="OO566_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6607377.1"
                     /translation="LSNQMSNYNNLANVGLGALQGQANAGQTYANNMSSIAQQSAALA
                     AANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT           84 a          105 c           90 g           92 t
ORIGIN      
        1 tcggagcgtt tgagttgacg ccgccgatac cagctaacaa tccgccattt gatccttgcc
       61 aagtagccat gattacccct taaaacaacg agccaagcaa tccgatacca gcaccaatgc
      121 cagcgcccca aggtgttgat gttcccaaaa ggctggcaag acctgcaccg gcaatcgcac
      181 cagacgttcc gccactaatt gctgtctgaa gacttgatgg tttgttggca ttagcagcgg
      241 caagagctgc gctttgctgt gcaatgctgc tcatgttgtt ggcgtatgtc tgcccggcgt
      301 ttgcctgacc ttgcagcgca ccaagcccaa cgtttgccag attgttgtaa ttgctcatct
      361 ggtttgataa c
//
LOCUS       DAJTXY010000372          368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTXY010000372 DAJTXY010000000
VERSION     DAJTXY010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.378"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..>368)
                     /locus_tag="OO566_004391"
     CDS             complement(<1..>368)
                     /locus_tag="OO566_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6607378.1"
                     /translation="EGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWAS"
BASE COUNT          106 a           96 c           81 g           85 t
ORIGIN      
        1 gcgatgccca ctcattgagg gtatagtagc cctcggtgga gtaacgatag ccaaccaggc
       61 gcaagctagt gtctgtgtcg tcaaatagtt ttgaatagag aaagcgatat gactgacccg
      121 tttctgtatc atcatggcta aaattcgcac gagcatgtgt cacatcgaaa gaaattgcgc
      181 ccaaagcgcc aagatcttta ccgacaccca gagctgcaga accataattt tcagcagcct
      241 gcatacctcc atataaagtg atcccgtggg aaaaaccatg aagtatttgc gcctgaagga
      301 cgtccggtgt aaatccgtct tcatcgcgca attcacccac gctgacatca acatctgtca
      361 gaccttca
//
LOCUS       DAJTXY010000373          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTXY010000373 DAJTXY010000000
VERSION     DAJTXY010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.379"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..205)
                     /locus_tag="OO566_004392"
     CDS             complement(<1..205)
                     /locus_tag="OO566_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607379.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDSCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWI"
BASE COUNT           80 a           85 c          107 g           88 t
ORIGIN      
        1 ttatccagtg acgataatcg agactgaggg ctttcttaat ctcgtatccg tgtctgcggt
       61 agcgctgaat cagccattca gcctgttctt cggtgcagga gtcgtgctga taccagtcag
      121 atttgaatgc atgagaacgc cgcccgtgcc tgctggcagg ggcggcagag ttatccgaat
      181 tgtaaaattt ggtatcgtgc gccatctgtt ttctctgctg gcgcagcagg tgccagttgt
      241 tcaggctgac ggatggattg taaaccagaa cgaccagaaa aaacaaaacc cgccgaagcg
      301 ggttaagtgc gggtgcgttg aggatgcctg acacatcagc ggtggcgagg gatttctccc
//
LOCUS       DAJTXY010000374          357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTXY010000374 DAJTXY010000000
VERSION     DAJTXY010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.380"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..>357)
                     /locus_tag="OO566_004393"
     CDS             complement(<1..>357)
                     /locus_tag="OO566_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6607380.1"
                     /translation="DLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELE
                     AVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLD
                     YVKTGQICQSQRHRPQ"
BASE COUNT          102 a           89 c           78 g           88 t
ORIGIN      
        1 tattgtggtc ggtgacgctg actctggcag atctgacccg tttttacata atcgagaaat
       61 gcgcgcaagc cgcaggacat atactggcgg tttgggtaat agacttggaa gcctggacgc
      121 tctgtgatcc agtcttccag tacacattct aaacgaccag tatcaaggta ctccttgatc
      181 tgttcataga gcagataccc gataccagca cccatcaaaa cggcctccag ttcagcatca
      241 acatcatcca gaatgatatt tcctgcgaca gcaatttcca gctcctttgc aaattgccag
      301 tggaaaggtt tgccgctggg ataacgaaaa acaacacatt gatgattaag cagatca
//
LOCUS       DAJTXY010000375          350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTXY010000375 DAJTXY010000000
VERSION     DAJTXY010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.381"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
BASE COUNT          114 a           69 c           82 g           85 t
ORIGIN      
        1 gttaaaatgt aatgaacgca tcccacacaa ttaaacacgg agtagtatga tggttaaaag
       61 ggtgaaactg gcaggtgtgg agcctcgcag ctttccggca tcatactgcc caatacggga
      121 tgcataaagc gtcctcagac tggagactcc acaaatgaaa tatacaccgg ttggcgttga
      181 tatggcaaaa catgtcattc agattcactt catcaatgag cacatgtcag cgccagtgat
      241 ataagacggt aatggggatg cggacgattt cctggactct cagaagatga aacatgttta
      301 ctgaagaaca aaaaattcgc gctattgagc tttatttcaa atatggtaag
//
LOCUS       DAJTXY010000376          348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTXY010000376 DAJTXY010000000
VERSION     DAJTXY010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.382"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..248
                     /locus_tag="OO566_004394"
     CDS             <1..248
                     /locus_tag="OO566_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607381.1"
                     /translation="KFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSI
                     PWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(250..>348)
                     /gene="ruvC"
                     /locus_tag="OO566_004395"
     CDS             complement(250..>348)
                     /gene="ruvC"
                     /locus_tag="OO566_004395"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="endonuclease; resolves Holliday structures; forms a
                     complex of RuvABC; the junction binding protein RuvA forms
                     a hexameric ring along with the RuvB helicase and
                     catalyzes branch migration; RuvC then interacts with RuvAB
                     to resolve the Holliday junction by nicking DNA strands of
                     like polarity; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6607382.1"
                     /translation="DALAIAITHCHVSQNAMQMSESRLNLARGRLR"
BASE COUNT           84 a           81 c           91 g           92 t
ORIGIN      
        1 caaaatttgt ctttgatatt tataaagagg cgctatgtat tccggtgggt gaaatagaga
       61 cgtttggaca actgttaaat agcaatccga atgcaaaact cactttctgg aaattctggt
      121 tcttaggttc tattccgtgg gagcgtaaaa ccgtcacgcc agccagtttg tggcatcatc
      181 cgggtttggt gttgattcac gcggagggag ttgaaacgcc tcagccagaa ctgaccgagg
      241 cggtataact taacgcagtc gccctctcgc caggttcagc cgcgattcgc tcatctgcat
      301 cgcattctga ctaacgtggc agtgggtgat ggcaatcgcc agcgcatc
//
LOCUS       DAJTXY010000377          346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTXY010000377 DAJTXY010000000
VERSION     DAJTXY010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.383"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            24..100
                     /locus_tag="OO566_004396"
     tRNA            24..100
                     /locus_tag="OO566_004396"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="OO566_004397"
     tRNA            143..218
                     /locus_tag="OO566_004397"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           98 a           72 c           87 g           89 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg agagttgttc gtgagt
//
LOCUS       DAJTXY010000378          346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTXY010000378 DAJTXY010000000
VERSION     DAJTXY010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.384"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            24..>346
                     /locus_tag="OO566_004398"
     CDS             24..>346
                     /locus_tag="OO566_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6607383.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFV"
BASE COUNT           94 a           76 c          105 g           71 t
ORIGIN      
        1 tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa cgtctgagag
       61 aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca gatattgcgc
      121 agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac atccgggata
      181 agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata tcagcgcagc
      241 cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg gtgacgctgc
      301 ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgt
//
LOCUS       DAJTXY010000379          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTXY010000379 DAJTXY010000000
VERSION     DAJTXY010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.385"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..>345)
                     /locus_tag="OO566_004399"
     CDS             complement(<1..>345)
                     /locus_tag="OO566_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6607384.1"
                     /translation="ELAATTLDYENYHIFVGTYPNDPDTQRDVDEVCARFPNVHKVVC
                     ARPGPTSKADCLNNVLDAITQFERSANFAFAGFILHGAEDVISPMELRLFNYLVERKD
                     LIQIPVYPFEREW"
BASE COUNT           88 a           86 c           94 g           77 t
ORIGIN      
        1 ccattcgcgt tcaaacggat acactgggat ctgaatcaga tctttacgct cgaccagata
       61 gttgaacaga cgcaattcca tcggtgaaat cacatcttcg gcgccatgca gaataaaacc
      121 agcaaaagcg aaattggcgc tacgttcaaa ctgggtgatg gcgtccagca cgttgttcag
      181 acagtcggct ttgctggtgg gaccaggacg cgcgcagact accttatgca cgttcggaaa
      241 gcgagcgcac acttcgtcaa catcacgttg agtatcgggg tcgttggggt aggtgccaac
      301 aaagatatga tagttttcgt agtcgagcgt ggtcgccgcc agctc
//
LOCUS       DAJTXY010000380          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTXY010000380 DAJTXY010000000
VERSION     DAJTXY010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.386"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="OO566_004400"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="OO566_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCR6607385.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           91 a           81 c          100 g           73 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatg
//
LOCUS       DAJTXY010000381          343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTXY010000381 DAJTXY010000000
VERSION     DAJTXY010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.387"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..193)
                     /locus_tag="OO566_004401"
     CDS             complement(<1..193)
                     /locus_tag="OO566_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607386.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQ"
     gene            complement(144..>343)
                     /locus_tag="OO566_004402"
     CDS             complement(144..>343)
                     /locus_tag="OO566_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6607387.1"
                     /translation="SYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPGSYCA
                     LSGVPDAHVSGAMPGLFIRTS"
BASE COUNT           81 a           89 c           92 g           81 t
ORIGIN      
        1 gctgacccgt cggtgcgaca tcatcagcgg tggctgtgta ggggactgcc tggtccctgt
       61 cgtcaaaaaa aacgtcacag tctactgcgc cactttcggt ataacgggga ttaatgattt
      121 ttttaatttc cacggtgcat tcctcacgat gtgcgaataa aaagcccggg cattgcgcca
      181 gagacatgtg catccggcac cccggacagg gcgcaatatg accccggtaa tgaatgctct
      241 gaacatcccg taatgaaaaa ttgtggggat gctatatacg ttccggtggg agtactgggc
      301 actgaaatcc ccaccggtcc cagtcgtgag cctctgtatg act
//
LOCUS       DAJTXY010000382          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTXY010000382 DAJTXY010000000
VERSION     DAJTXY010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.388"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..298)
                     /locus_tag="OO566_004403"
     CDS             complement(<1..298)
                     /locus_tag="OO566_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6607388.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFAK"
BASE COUNT           75 a          101 c           81 g           84 t
ORIGIN      
        1 ccttcgcaaa ataagcggaa gcctggcgaa ggatatcgtt actgcggcgc agttcacgat
       61 tttcacgctc cagctctttc agacgctgac gttcagcggt ggtgagccct ccatcaccgc
      121 ccccggtatc ccgctcatgc tggcgaaccc agacacgcag agtctccggc gtacagccaa
      181 tctttggagc aatggaacaa attgtcgccc attgtgagtc atattcgccc tgactttcca
      241 gaaccatacg gactgcccgt tgacggactt cgggggaaaa acgagtattt ttagtcatcc
      301 tgtttacctc tttctcagga agtttagtct ccaggattcc c
//
LOCUS       DAJTXY010000383          337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTXY010000383 DAJTXY010000000
VERSION     DAJTXY010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.390"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..>337)
                     /locus_tag="OO566_004404"
     CDS             complement(<1..>337)
                     /locus_tag="OO566_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6607389.1"
                     /translation="TLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWA
                     IDNMLTDIPYLPENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQR
                     LTSSPDYHGP"
BASE COUNT           97 a           56 c           82 g          102 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgt
//
LOCUS       DAJTXY010000384          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTXY010000384 DAJTXY010000000
VERSION     DAJTXY010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.391"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
BASE COUNT           97 a           80 c           61 g           96 t
ORIGIN      
        1 agcaaaactc tgatgacctg aatcaccttt accaatattg tatgaaaaaa cacgcacgac
       61 taattgacaa taattttcat ttccattaaa aaagatcgcg tagcattctt aattcatgag
      121 gaactttacc cgccagcaat ctgagtagcg aaagctgttc atccccaatg tttttgctgg
      181 cgggtccttt tttctacggt cctcctcccc ggaggggggg catttcatta tttttctaat
      241 cgttactggt gagaaggcgg aagaaaaagc cagttcttcg cccctaaata acagcatgat
      301 tccagtccat gcaaaatctg atttccagct caca
//
LOCUS       DAJTXY010000385          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTXY010000385 DAJTXY010000000
VERSION     DAJTXY010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.392"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..>332)
                     /locus_tag="OO566_004405"
     CDS             complement(<1..>332)
                     /locus_tag="OO566_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6607390.1"
                     /translation="INQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPND
                     ERLQQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISA
                     VTLLDDGY"
BASE COUNT           87 a           69 c           72 g          104 t
ORIGIN      
        1 aatatccatc atcaagaagc gtcactgcag aaataacgtg catttgctga acccagtggc
       61 gaaatgttaa tcccgtctct ttaacaaata agcgcatgag tgtacggcta ctgacgttaa
      121 ttttatccgc tagattatcc agtgttggca gatacccttg gttgtttagc aaattctcac
      181 aaagctgctg tagccgctca tcatttggcc aagggagttc aagtgacatt ttcaccccca
      241 tttttatttc ctgaagtatc agtgtctcga gggctgaaaa acgtaactgt tgtttatctg
      301 tggtactttg cgacggtgga agttggttaa tt
//
LOCUS       DAJTXY010000386          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTXY010000386 DAJTXY010000000
VERSION     DAJTXY010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.393"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
BASE COUNT          109 a           60 c           62 g          101 t
ORIGIN      
        1 gactggttta agctccattt tcaaaataat atatcagggt ttgttactaa taatttctgg
       61 aaacagaaaa agtagagcag taattctatt tcagagagga gtaactccat ctatcctcga
      121 tacatactga acgttatcgt gatatattta tcatgcgttc ttcaccatta ttcgagtgca
      181 gggacgcaat gaccttaaat accttttagt agaatatatt gctgacccac aaatatcagg
      241 atggtattga caagtcatag atggagaaat atatgactga attaccattc tctggaataa
      301 cagcagactc cttcccatag acggcttagg gg
//
LOCUS       DAJTXY010000387          330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTXY010000387 DAJTXY010000000
VERSION     DAJTXY010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.394"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            99..>330
                     /locus_tag="OO566_004406"
     CDS             99..>330
                     /locus_tag="OO566_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6607391.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          117 a           72 c           46 g           95 t
ORIGIN      
        1 ccacttcaga aaacaaactt atttttatta aaatcaattc tttttctttg aacattcaac
       61 tgcgatattt gtaaaaacat taaacaggca acaataaaat gacattacca acaactattt
      121 attcattccc cgcatattta ccccgttttt cgtccaccga caaaccagca aaattaaaat
      181 ttcatcaata cgcacgcgct acattactgt caaatcgtgg aagagatcat aattgcgatg
      241 gcagacgtac tgttgagatc cacaaactag atctctctga ctggcaagca ttcaataagt
      301 tagcaacgaa gtgtaatgct tacgatggag
//
LOCUS       DAJTXY010000388          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTXY010000388 DAJTXY010000000
VERSION     DAJTXY010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.395"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..287
                     /locus_tag="OO566_004407"
     CDS             <1..287
                     /locus_tag="OO566_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR6607392.1"
                     /translation="YSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDS
                     VDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
BASE COUNT          110 a           74 c           64 g           79 t
ORIGIN      
        1 agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat gccaaaaaag
       61 gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc ttcgggccag
      121 gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt cacggacaat
      181 actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga aacgacgccc
      241 gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg tcaaccgacg
      301 gggactatcc ccgtcataga cgttaac
//
LOCUS       DAJTXY010000389          324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTXY010000389 DAJTXY010000000
VERSION     DAJTXY010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.396"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>324
                     /locus_tag="OO566_004408"
     CDS             <1..>324
                     /locus_tag="OO566_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6607393.1"
                     /translation="SLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKDIIG
                     VWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVARQINPYIKVDIPETTRGALYSF
                     VYNVG"
BASE COUNT           90 a           75 c           80 g           79 t
ORIGIN      
        1 catcattacg caaggctgtt gcagttgcta ttggtggcgg ggctgttgct atagcatctg
       61 tgttaatcac tggcccaggt ggtaacgatg gtctggaagg tgtcagctac ataccataca
      121 aagatatcat tggtgtatgg actgtatgtc acggacacac cggaaaagac atcatgcctg
      181 gtaaaacgta taccgaagca gaatgcaaag ctctcctgaa taaagacctt gtcactgtcg
      241 ccagacaaat taacccgtac atcaaagtcg atataccgga aacaacgcgc ggcgctcttt
      301 actcgttcgt ttacaacgtg ggcg
//
LOCUS       DAJTXY010000390          319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTXY010000390 DAJTXY010000000
VERSION     DAJTXY010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.397"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(97..167)
                     /locus_tag="OO566_004409"
     tRNA            complement(97..167)
                     /locus_tag="OO566_004409"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Val,seq:tac)
     gene            complement(171..246)
                     /locus_tag="OO566_004410"
     tRNA            complement(171..246)
                     /locus_tag="OO566_004410"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Thr,seq:tgt)
BASE COUNT           88 a           87 c           74 g           70 t
ORIGIN      
        1 acagcaattt tgcaaaaaag acgagcattc aaaacgcaaa cgtctttcag tcactccgtg
       61 ctttcccatc atcgcagact gaaagacttt aacttgagcg gcagcgggaa tcgaacccgc
      121 atcatcagcc tgtaaggctg aggtaatagc cattatacga tgcccgcata tggtgccgac
      181 taccggaatc gaactggtga cctgatgatt acaagtcagt tgctctgcct gctgagctaa
      241 gtcggcgcta gcccaccacc gagaactcga acctcgcacc gtcaacttag aaggttgatg
      301 ctctatccgg atgagctag
//
LOCUS       DAJTXY010000391          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTXY010000391 DAJTXY010000000
VERSION     DAJTXY010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.398"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..>312)
                     /locus_tag="OO566_004411"
     CDS             complement(<1..>312)
                     /locus_tag="OO566_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6607394.1"
                     /translation="MDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTK
                     REEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAM
                     QI"
BASE COUNT           72 a           97 c           59 g           84 t
ORIGIN      
        1 gatttgcatc gccgtttttc tcactgtctc cgttatgatg tacaccactt cttccagtaa
       61 ccgccgtttc gccggactca aaaaaccgta gtacctcacc atacggaacc ccttatccgc
      121 cacatgccag gagaaccttt ccatgaactc atctccactc atcaacaggt attcttcccg
      181 ttttgttcgg tgactgttgt aacgcagacc gatttcatcc tgaccggcat aatgctccag
      241 acgactcatc ggcaccggtg gctttttcag gtaagagcca aagtacaccg ccacatgggt
      301 ggcattatcc at
//
LOCUS       DAJTXY010000392          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTXY010000392 DAJTXY010000000
VERSION     DAJTXY010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.400"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..>304)
                     /locus_tag="OO566_004412"
     CDS             complement(<1..>304)
                     /locus_tag="OO566_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6607395.1"
                     /translation="MAAMFHQQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQ
                     GYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHL"
BASE COUNT           85 a           69 c           62 g           88 t
ORIGIN      
        1 tagatgtttt ttttcatcat aaaccgggaa aagtggtata atcatagcct gactgatgtt
       61 catcagacgt ccaatgattg gtaatgtcgc tttataggtc gcaaaaaaat cagcaaattc
      121 actgtattca ggaccatgat cctgatctgg gaggtaatat ccccagtatc cctggcgtac
      181 tgactgaata aatggtttta tcccatcctc ccgggcatgt aagcgccccc cgaatttacg
      241 ccgtactgaa ttccagacat aatcaatcac tggatttcgt tgctgatgaa acatggcggc
      301 catt
//
LOCUS       DAJTXY010000393          303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTXY010000393 DAJTXY010000000
VERSION     DAJTXY010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.401"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            <1..>303
                     /locus_tag="OO566_004413"
     CDS             <1..>303
                     /locus_tag="OO566_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6607396.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRR"
BASE COUNT           91 a           52 c           94 g           66 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggg
//
LOCUS       DAJTXY010000394          297 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTXY010000394 DAJTXY010000000
VERSION     DAJTXY010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.403"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..>297)
                     /locus_tag="OO566_004414"
     CDS             complement(<1..>297)
                     /locus_tag="OO566_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6607397.1"
                     /translation="GRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRS
                     YRGEVGQTAPNVLQRDFKATRPNEKWVTDFTEFAVNGRKLYLSPVIDLFNNEVIS"
BASE COUNT           70 a           72 c           69 g           86 t
ORIGIN      
        1 agaaataact tcgttgttga agagatctat tactggagac aaatacagct tgcgcccatt
       61 gactgcaaat tcagtaaaat cggtaaccca cttctcgttt ggccgcgtag ccttgaaatc
      121 tctttggaga acattggggg cggtttgccc tacctctcct ctgtaagagc ggtatcgctt
      181 gaccttaatc gctgctttaa gtgagagggt tcccatcagg cgctgaacag ctttatggtt
      241 aatctgtttc ccttctcgat gaagagacag cgttacccta cggtatccgt atcggcc
//
LOCUS       DAJTXY010000395          297 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTXY010000395 DAJTXY010000000
VERSION     DAJTXY010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.denovo.404"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            26..>297
                     /locus_tag="OO566_004415"
     CDS             26..>297
                     /locus_tag="OO566_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6607398.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTP"
BASE COUNT           78 a           63 c           77 g           79 t
ORIGIN      
        1 atgtttttaa attgagggca ttattatgaa aaaaataccc tgcgtgatga tgcgaggtgg
       61 aacctcgagg ggcgcgttcc tgttagcgga acatttaccc gaagatcaaa cgcagcgcga
      121 taaaatattg atggcaatta tgggttccgg taacgatctg gaaattgacg gtattggcgg
      181 cggtaatcca ctgaccagta aagtcgccat tattagccgt tccagcgatc cgcgtgctga
      241 tgtcgattat ctgtttgctc aagttatcgt ccatgagcaa cgtgtcgata ccacgcc
//
LOCUS       DAJTXY010000396         6901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.guided.166, whole genome shotgun sequence.
ACCESSION   DAJTXY010000396 DAJTXY010000000
VERSION     DAJTXY010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.guided.166"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            1..1040
                     /locus_tag="OO566_004416"
                     /pseudo
     CDS             1..1040
                     /locus_tag="OO566_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1102..1716
                     /locus_tag="OO566_004417"
                     /pseudo
     CDS             1102..1716
                     /locus_tag="OO566_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1773..3230)
                     /gene="pepD"
                     /locus_tag="OO566_004418"
     CDS             complement(1773..3230)
                     /gene="pepD"
                     /locus_tag="OO566_004418"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6607399.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3492..3950
                     /gene="gpt"
                     /locus_tag="OO566_004419"
     CDS             3492..3950
                     /gene="gpt"
                     /locus_tag="OO566_004419"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6607400.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4042..5286
                     /gene="frsA"
                     /locus_tag="OO566_004420"
     CDS             4042..5286
                     /gene="frsA"
                     /locus_tag="OO566_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6607401.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5344..5745
                     /gene="crl"
                     /locus_tag="OO566_004421"
     CDS             5344..5745
                     /gene="crl"
                     /locus_tag="OO566_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6607402.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5784..6839)
                     /gene="phoE"
                     /locus_tag="OO566_004422"
     CDS             complement(5784..6839)
                     /gene="phoE"
                     /locus_tag="OO566_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6607403.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1664 a         1715 c         1784 g         1738 t
ORIGIN      
        1 caattatatt cgtcccttat ccgatgcggt atttaccatc gcatctgatg acctgtggat
       61 cgagagttta gcgatccaac aattacacac cacggcaaat ttacccaaca tgcagcgcgt
      121 agtggggatg ccagatttac accccggacg cggctacccg attggcgcag cgttcttctc
      181 cgcaggccgt ttttacccag cactggtcgg caatgatatc ggctgtggca tggcgctatg
      241 gcaaacagat attctcgctc gcaaatacaa cgccgataag tttgaaaagc gattatcagc
      301 tttggatgac gttgctgaag aaagctggct ggaggaaaac ctgccgtcag catttgcaca
      361 gcatccgtgg cgcagctcgc ttggctctat cggtggtggc aatcatttcg ctgaactgca
      421 acaagttgat caaatcatca acgctgaact gtttgcactg gcaggtctgg atgcgcagca
      481 tctgcaactg ctggttcata gcggctcgag ggagctaggg cagtctattt tacagcggca
      541 tattgcctcg ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc
      601 gcgttttaat cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag
      661 tccggttctg gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg
      721 ggtcttacat cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc
      781 acgcggtgat tactccaggc tggttcaggc ccgtcaggag tgaggaaaca ttgcattcgc
      841 tggcgcatgg ggctgggcgt aaatgggggc gcaccgagtg taaagggcgt ctggcagcga
      901 aatacacagc gacgcaactc tcgcgtactg aacttggcag tcgggtaatt tgtcgcgata
      961 aacaactcat cttcgaagaa gcgccacaag cctataaatc ggctgaaagt gtggtgcaat
     1021 gtctgggcag gccggattaa ttattcctgt cgcgcggctg cgtccggtgc taacgctcaa
     1081 aaacagtgga gggaaaaaag gatgatcttg ctataactct cctcagcgca ggggccggaa
     1141 gaatgttgtc tcgcagtgaa aaaggcgctg gacaggctaa ttaaagaagc tgcccgacaa
     1201 gacgtcgcgg taacggtgct ggaaacagaa acaggccgct actctgacac gctgcgttcg
     1261 gcgctgattt ctctggatgg cgacaacgca tgggcgttaa gcgaaagctg gtgtggcact
     1321 attcagtgga tttgcccgag cccgtatcgc ccacatcacg ggcgtaaaaa ctggtttctg
     1381 ggcattgggc gttttaccgc tgatgagcag gagcaatccg atgcgatccg gtatgagacg
     1441 ctgcgttcgt cggggccggg cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc
     1501 acgcatttgg catccggtat tagcgtgaag gttcagtcag agcgcagcca gcatgctaac
     1561 aaacggctgg cgcgattgct gattgcctgg aagctggatc agcagcaata ggaaaatagc
     1621 gcggtgctga aatcacagcg gcgaatgttc catcaccaga ttgaacgtgg caacccgcga
     1681 cgaacgttta cagggatggc ttttatcgaa ggataatgaa ggatgaaact gccggaaggc
     1741 gattaaacgc catccggcag caaatcaaat aattacttcg ccggaatttc tttcagcagt
     1801 tcagtcagca gtgtccagta atgacctacg ctttcgatgt gaacttgctc atccggagag
     1861 tgtggaccgg tgatagttgg cccgatagaa accatgtcca tttccggata cggttttttg
     1921 aacagaccac attccaggcc cgcgtggata atctggatgt tcggcgtctt gttgaacagg
     1981 cgctgatagg tttcacgtac cagatgcatc accggagaat tagcgtccgg ctgccagcca
     2041 ggatatgcgc ctttcgcttc ggttttcgcg ccagccagtt tacccagcga atccagcatg
     2101 ctcaccacgt agtctttacc gctgtcgatc agtgaacgga tcaggcagtg aatttctacg
     2161 ttattgtcag tcatggtcac cacaccgacg ttcagggagg tttcgaccac accttttgcc
     2221 acgtcggaat tgcggatcac accgttcggg gtggcgttca gcagacgaat aaaggtatcg
     2281 cgagatttcg caatcagggc agctttatcg ttcgctacag agtccagcaa caaggccaga
     2341 tttttctctt tctctgccag ctcgtttttc aggatctcct gataggtatt caccagagat
     2401 ttcagtgcgt cgactttatc agctgtgaca gcaatggtcg caaaggcttc acgcgggatg
     2461 gcgttacgca gtgtgccgcc gttgaaatcg ataaggcgca ggtccagttc ttctgcatga
     2521 cccgccagga agcgcaccag cagtttgttg gcattaccca ggccaacgtg gatttcaccg
     2581 ccggagtgac cgcctttcag accttttaag gttaacttga aggtttcaaa accagctgga
     2641 accgcttcac gatctaaatg caggttggag gtgaagtcga taccccccgc acaacccatg
     2701 tagatttcac cttcttcttc ggagtcggtg ttaatcagaa tatcagcctg caaccagttg
     2761 ctctgtaagc cgaacgcacc gtccataccg gcttcttcgg ttatggtcag cagcacttcc
     2821 agcgggccgt gaaccacgtt ttcgtcagcc agaaccgcca gtgcagaggc cataccaatg
     2881 ccgttatccg cacccagcgt ggtgccgcgc gctttaaccc attcgccatc aatataaggc
     2941 tggataggat ctttcgtgaa gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg
     3001 aggtgggcct gtaagacgac cggtttacga ttttccatac ctgcggtagc aggtttacga
     3061 atcaggatat tacctacctg atcgcgttcg acatggaaac ctttctcttt tgcccaacca
     3121 acaatgtatt cagcgagttg ctcttcatga taggacgggt gaggaataga acagattttg
     3181 gcaaaaatat cccacagcgg ctgtggagat aattgagaca gttcagacac gttaagtctc
     3241 cttgtcgatc acccgcaaaa cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa
     3301 cacaagacag gcttgcgaga tatgtattga gaataccact ttatcccgcg tcagggagag
     3361 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatatct
     3421 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
     3481 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
     3541 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
     3601 gtggcggtct ggtaccgggg gcgttactgg cgcgtgaact gggtattcgt catgtcgata
     3661 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
     3721 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
     3781 ctgttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
     3841 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggatcgaac
     3901 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
     3961 gcctggcatt gccgggcgtt gttcttttta acttcaggca ggttacaata gtttccagta
     4021 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
     4081 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
     4141 cctgtgcagt cggctcttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
     4201 ctgatgtgga tctggcgcgg tattgaccca cgtgaaatcc tcgacgtcca ggcacgtatt
     4261 gtgatgagcg atgctgaacg taccgacgat gatttatacg ataccgtgat tggctaccgt
     4321 ggtggcaact ggatttatga gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt
     4381 acggaagaag atccgcaact cagtggtcga cactggctgc atgcggctac gttgtacaac
     4441 attgccgcct atcctcatct gaaaggagat gacctggccg agcaagcgca ggctttatca
     4501 aaccgcgcct atgaagaggc cgcccagcgt ctgccgggca cgatgcggca gatggagttt
     4561 accgtacccg gcggtgcgcc catcaccggc tttttgcata tgccgcaagg cgatggcccg
     4621 ttcccgacag tattaatgtg tggtggtctg gatgcgatgc agacggacta ttacagcctg
     4681 tatgaacgtt attttgcgcc gcgcggcatt gcgatgctga ctattgatat gccgtcggtg
     4741 ggcttttctt caaaatggaa gctcacccag gactccagcc tgttgcatca gcacgtctta
     4801 aaggcgctgc ctaacgtacc gtgggtggat cacactcgcg tcgcggcctt tggtttccgt
     4861 ttcggcgcta acgttgccgt gcgtctggca taccttgaat cgccgcgtct gaaagcggtt
     4921 gcctgtcttg gtccggtagt tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg
     4981 ccggaaatgt atcttgacgt tctggcgagt cgtttgggga tgcatgatgc ttccgacgaa
     5041 gcgttacgcg tggagctgaa tcgctattca ttaaaagtgc aaggattgct ggggcgtcgc
     5101 tgcccaacgc caatgttatc aggctactgg aagaacgatc cgttcagccc ggaagaggac
     5161 tcacgcttaa tcacctcatc atctgctgac ggtaaattat tagagatccc atttaacccg
     5221 gtgtatcgga attttgacaa aggtcttcag gaaatcaccg gctggatcga aaaacgcttg
     5281 tgttaaaaat ttgctaaatt ttgccaattt ggtaaaacag ttgcatcaca acaggagata
     5341 gcaatgacgt taccgagtgg acacccgaag agcagattga tcaaaaaatt taccgcacta
     5401 ggcccgtata ttcgtgaagg taagtgcgaa gataatcgat tctttttcga ttgtctggct
     5461 gtatgcgtca acgtgaaacc ggcaccggaa gtgcgtgaat tctggggctg gtggatggag
     5521 ctggaagcgc aggaatcccg ttttacctac agctaccagt ttggtctgtt cgataaagca
     5581 ggcgactgga agagtgttcc ggtaaaagat actgaagtgg ttgaacgact ggagcacacc
     5641 ctgcgtgagt ttcacgagaa gctgcgtgaa ctgctgacga cgctgaatct gaagctggaa
     5701 ccggcggatg attttcgtga tgaaccggtg aagttaacgg cgtgagtgaa atgtgccgga
     5761 tgcatcacat ccggcaatat tcattaaaac tgatacgtca tcccaaccgc gacagtatca
     5821 tcattattaa tattcagctt attatcgcta tccagtttgt tgattttata atcaacaaac
     5881 gctgacatat ttttgttgaa ataatacgta gcaccgacgt cgatataatt gaccagatct
     5941 tcatcaccga tgccctcaat atctttccct ttcgataaga cataacccag cgatggacgc
     6001 agaccaaagt caaactggta ttgagcgacc gcttcaaagt tctgtgtctt attggcaaag
     6061 ccgccagtta ttggcgtcat tttgcgtgtt tcagaataga aagttgccag ataaatatta
     6121 ttggcatcgt atttcagacc tgttgcccat gcttctgcac gcttgcctgt gccacggctt
     6181 tgcaggttct gctcgttggt gcgatctgag ttggtatagg ccccactaat ggcgaaatcg
     6241 ctgccgccaa agtcatatgt caatgacgtg ccgaagccat cgccgttttg ctttttaacg
     6301 tcgcggtttt cgtttttccc ttgatattgc agggttaagt tcaggccatc gataacgccg
     6361 aagaagtcgg tgttccgata cgtcgccaga ccgctggcgc gtttggtcat aaagttgtcg
     6421 gtctgcgccg aggaatcgcc accaaattcc gggaacatat cggtccaggc ttccacgtca
     6481 tacaacgcgc ccaggttacg accataatcg aaagaaccca aatctttata tttcaacccg
     6541 gcaaaagcga gacgcgtttt ttgctgtgca gtatcactct ccgctttatt tccggcaaac
     6601 tccgcttccc accgaccata accagtcagt tgatcgttaa tttgagtttc acctttaaaa
     6661 ccaaaacgga tataactctg gtcgccatct ttactggcgt tatcactcat ataatgcatg
     6721 gctttaactt tgccatagac atccagttta ttaccgtctt tattatatat ttctgcagcc
     6781 tgtacagatg cagatgccac aatgcccatc accactaatg ccagagtgct ctttttcatt
     6841 ttcattcctg attttaatta acgcgcgaat attcagcggg agagccccgt tgaaaacagg
     6901 a
//
LOCUS       DAJTXY010000397         6744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.guided.168, whole genome shotgun sequence.
ACCESSION   DAJTXY010000397 DAJTXY010000000
VERSION     DAJTXY010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.guided.168"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..607)
                     /gene="epmA"
                     /locus_tag="OO566_004423"
     CDS             complement(<1..607)
                     /gene="epmA"
                     /locus_tag="OO566_004423"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6607404.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEP"
     gene            932..2740
                     /gene="frdA"
                     /locus_tag="OO566_004424"
     CDS             932..2740
                     /gene="frdA"
                     /locus_tag="OO566_004424"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6607405.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            2733..3467
                     /gene="frdB"
                     /locus_tag="OO566_004425"
     CDS             2733..3467
                     /gene="frdB"
                     /locus_tag="OO566_004425"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6607406.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3478..3873
                     /gene="frdC"
                     /locus_tag="OO566_004426"
     CDS             3478..3873
                     /gene="frdC"
                     /locus_tag="OO566_004426"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6607407.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3884..4243
                     /gene="frdD"
                     /locus_tag="OO566_004427"
     CDS             3884..4243
                     /gene="frdD"
                     /locus_tag="OO566_004427"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6607408.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            4306..5439
                     /locus_tag="OO566_004428"
     CDS             4306..5439
                     /locus_tag="OO566_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6607409.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            5528..6061
                     /gene="blc"
                     /locus_tag="OO566_004429"
     CDS             5528..6061
                     /gene="blc"
                     /locus_tag="OO566_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6607410.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6058..6287)
                     /locus_tag="OO566_004430"
                     /pseudo
     CDS             complement(6058..6287)
                     /locus_tag="OO566_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(6463..6609)
                     /gene="ecnB"
                     /locus_tag="OO566_004431"
     CDS             complement(6463..6609)
                     /gene="ecnB"
                     /locus_tag="OO566_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6607411.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT         1679 a         1746 c         1774 g         1545 t
ORIGIN      
        1 ttggctctac gccaaaggta aacagcaatt gtagcaacgt gtcgcggtct tcttcggtat
       61 cagcaacatt gctcaaatcc agtttcgctg cgacttcccg cagttgcgtt ttgtcggcag
      121 agagcgggtc aatttccaga taacgcaaga aagcttgttg ataagaaagg ctttctgctg
      181 ccgggcagtc cagcacctgt tgtaagagat cgtccacctc gttcatcaac cggtacatat
      241 catagtgcgg tcgataccac tccagcatag tgaactcagg gttgtgataa cgccccatct
      301 cttcattacg gaagctgcgg cacagctgga ataccggccc acaaccagca accagcaggc
      361 gtttcatatg gtattccggg ctggtcatta accagagatt catcccctgc gaatgcccgg
      421 ggccaacgaa acgtgtctca aacgggacca aatgaatatc ggttaccgtc gcctggctca
      481 tacaaggcgt ctccacctcc agcactccac gatcggcaaa gaaacgacgg atctccgcca
      541 taatcgccgc gcgttttaat aagttaggaa tggatgcgct cggctgccag gatgccgttt
      601 cgctcatagt taaatctcca gtttttgaca agggcacgaa gtctactcgc aacgcgacgg
      661 cgagacaaat tttacgcagg aatcaaacag cggtgggcag tgactaaaaa aagcacgatc
      721 tgatggttta gtaattaaat taatcatctt cagtgataat ttagccctct tgcgcactaa
      781 aaaaatcgat ctcgtcaaat ttcagactta tccatcagac tatactgttg tacctataaa
      841 ggagcagtgg aatagcgttc gcagaccgta actttcaggc acttaccctg aagtacgggg
      901 ctgtgggata aaaacaatct ggaggaatgt cgtgcaaacc tttcaagccg atcttgccat
      961 tgtaggcgcc ggtggcgcgg gattacgtgc tgcaattgct gccgcgcagg caaatccaaa
     1021 tgcaaaaatc gcactaatct caaaagtata cccgatgcgt agccataccg ttgctgcaga
     1081 agggggctcc gccgctgtcg cgcaggatca tgacagcttc gaatatcact ttcacgatac
     1141 agtagcgggt ggcgactggt tgtgtgagca ggatgtcgtg gattatttcg tccaccactg
     1201 cccaaccgaa atgacccaac tggaactgtg gggatgccca tggagccgtc gcccggatgg
     1261 tagcgtcaac gtacgtcgct tcggcggcat gaaaatcgag cgcacctggt tcgccgccga
     1321 taagaccggc ttccatatgc tgcacacgct gttccagacc tctctgcaat tcccgcagat
     1381 ccagcgtttt gacgaacatt tcgtgctgga tattctggtt gatgatggtc atgttcgcgg
     1441 cctggtagca atgaacatga tggaaggcac gttggtgcag atccgtgcta acgcggtcgt
     1501 tatggctacc ggcggtgcgg gtcgcgttta tcgttacaac accaacggcg gcatcgttac
     1561 cggtgacggt atgggtatgg cgctaagcca cggcgttccg ctgcgtgaca tggaattcgt
     1621 tcagtatcac ccaaccggtc tgccaggttc cggtatcctg atgaccgaag gctgccgtgg
     1681 tgaaggcggt attctggtca acaaaaatgg ctaccgttat ctgcaagatt acggcatggg
     1741 cccggaaact ccgctgggcg agccgaaaaa caaatatatg gaactgggtc cacgcgacaa
     1801 agtttctcag gccttctggc acgaatggcg taaaggcaac accatctcca cgccgcgtgg
     1861 tgatgtggtt tatctcgacc tgcgtcacct cggcgagaaa aaactgcatg aacgtctgcc
     1921 gttcatctgc gaactggcga aagcgtacgt tggcgtcgat ccggttaaag aaccgattcc
     1981 ggtacgtccg actgcacact acaccatggg cggtatcgaa accgatcaga actgtgaaac
     2041 ccgcattaaa ggtctgttcg ccgtgggtga atgttcctct gttggtctgc acggtgcaaa
     2101 ccgtctgggc tccaactccc tggcggaact ggtggtcttc ggccgtctgg ccggtgaaca
     2161 agcgacagag cgtgcagcaa ctgccggtaa tggcaacgaa gcggcaattg aagcgcaggc
     2221 agctggcgtt gaacaacgtc tgaaagatct ggttaaccag gatggcggcg aaaactgggc
     2281 gaagatccgc gacgaaatgg gcctggcaat ggaagaaggt tgcggtatct accgtacgcc
     2341 ggaactgatg cagaaaacca tcgacaagct ggcagagctg caggaacgct tcaagcgcgt
     2401 gcgcatcacc gacacctcca gcgtgttcaa caccgacctg ctctacacca ttgaactgag
     2461 ccacggtctg aacgttgctg aatgtatggc gcactccgca atggcacgta aagagtcccg
     2521 cggcgcacac cagcgtctgg acgaaggttg caccgagcgt gacgacgtca actttctcaa
     2581 acacaccctc gccttccgcg atgctgatgg cacgactcgc ctggagtaca gcgacgtgaa
     2641 gattactacg ctgccgccag ctaaacgcgt ttacggtggc gaagcggatg cagccgataa
     2701 ggcggaagca gccaataata aggagaaggc gaatggctga gatgaaaaac ctgaaaattg
     2761 aggtggtgcg ctataacccg gaagtcgata ccgcaccgca tagcgcattc tatgaagtgc
     2821 cttatgacgc aactacctca ttactggatg cgctgggcta catcaaagac aacctggcac
     2881 cggacctgag ttaccgctgg tcctgccgta tggcgatttg tggttcctgc ggcatgatgg
     2941 ttaacaacgt gccaaaactg gcatgtaaaa ccttcctgcg tgattacacc gacggtatga
     3001 aggttgaagc gttagctaac ttcccgattg aacgcgatct ggtggtcgat atgacccact
     3061 tcatcgaaag tctggaagcg atcaaaccgt acatcatcgg caactcccgc accgcggatc
     3121 agggtactaa catccagacc ccggcgcaga tggcaaagta tcaccagttc tccggttgca
     3181 tcaactgtgg tctgtgctac gccgcgtgcc cgcagtttgg cctgaaccca gagttcatcg
     3241 gtccggctgc cattacgctg gcgcatcgtt ataacgaaga tagccgcgac cacggtaaga
     3301 aggagcgtat ggcgcagttg aacagccaga acggtgtatg gagctgtact ttcgtgggct
     3361 attgctccga agtctgcccg aaacacgtcg atccggctgc ggccattcag cagggcaaag
     3421 tagaaagttc gaaagacttt cttatcgcga ccctgaaacc acgctaagga gtgcaacatg
     3481 acgactaaac gtaaaccgta tgtacggcca atgacgtcca cctggtggaa aaaattgccg
     3541 ttttatcgct tttacatgct gcgcgaaggc acggcggttc cggctgtgtg gttcagcatt
     3601 gaactgattt tcgggctgtt tgccctgaaa aatggcccgg aagcctgggc gggattcgtc
     3661 gactttttac aaaacccggt tatcgtgatc attaacctga tcactctgac ggcagctctg
     3721 ctgcacacca aaacctggtt tgaactggca ccgaaagcgg ccaatatcat tgtaaaagac
     3781 gaaaaaatgg gaccagagcc aattatcaaa agtctctggg cggtaactgt ggttgccacc
     3841 atcgtaatcc tgtttgttgc cctgtactgg taaggagcct gagatgatta atccaaatcc
     3901 aaagcgttct gacgaaccgg tattctgggg cctcttcggg gccggtggta tgtggagcgc
     3961 aattattgcg ccggtgatga tcctgctggt gggtattctg ctgccactgg ggctgtttcc
     4021 gggtgatgcg ctgagctacg agcgcgttct ggcgttcgcg cagagcttca ttggtcgcgt
     4081 attcctgttc ctgatgatcg ttctgccgct gtggtgtggt ttacaccgta tgcaccacgc
     4141 gatgcacgat ctgaaaatcc acgtgcctgc gggcaaatgg gttttctacg gtctggctgc
     4201 tatcctgaca gttgtcacgc tgattggtgt cgttacaatc taacgcatcg ccaatgtaaa
     4261 tccggcccgc ctatggcggg ccgttttgta tggaaaccag accttatgtt caaaacgacg
     4321 ctctgcgcct tattaattac cgcctcttgc tccacatttg ctgcccctca acaaatcaac
     4381 gatattgtgc atcgcacaat taccccgctt atagagcaac aaaagatccc cggtatggcg
     4441 gtggcggtaa tttatcaggg taaaccttat tactttactt ggggctatgc ggacatcgcc
     4501 aaaaagcagc ccgtcacaca gcaaacgttg tttgagttag gttcggtcag caaaacattt
     4561 acgggcgtgc ttggtggcga cgctattgct cgaggggaaa tcaagttaag cgatcccgca
     4621 acaaaatact ggcctgaact taccgctaaa cagtggaatg ggatcacact attacatctt
     4681 gcgacctaca ccgctggcgg cctgccattg caggtgccgg atgaagtgaa atcctcaagc
     4741 gacttgctgc gcttctatca aaactggcag cctgcatggg ccccaggaac acaacgtctg
     4801 tatgccaact ccagtatcgg tttgttcggc gcactggctg tgaagccgtc tggtttgagt
     4861 tttgagcagg cgatgcaaac tcgtgtcttc cagccactca aactcaacca tacgtggatt
     4921 aatgtacctc ccccagaaga aaagaattac gcctggggat atcgcgaagg taaggcagtg
     4981 catgtttcgc caggggcgtt agatgctgaa gcttatggtg tgaagtcgac cattgaagat
     5041 atggcccgct gggtgcaaag caatttaccc ccccttgata tcaatgagaa aacgcttcaa
     5101 caagggatac aactggcaca atctcgctac tggcaaaccg gcgatatgta tcagggcctg
     5161 ggctgggaaa tgctggactg gccggtcaat cctgacagca tcattaacgg cagtgacaat
     5221 aaaattgcac tggcagcacg cccggtaaaa gcgattaagc ccccaactcc tgcagtacgc
     5281 gcatcatggg tacataaaac gggggcgacc ggcggatttg gtagctatgt cgcgtttatt
     5341 ccagaaaaag agctgggtat cgtgatgctg gctaacaaaa actatcccaa tccagagaga
     5401 gtcgccgccg cctgtcagat tctcaacgct ctacagtaaa atttcatcgg gtccgaattt
     5461 tcggaccttt tctgcgcttt tccttgctgt catctacact tagaaaaaaa ccagtaagga
     5521 aacatttatg cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc
     5581 ctgtagttct cctacgccgc cgcgtggcgt gaccgtagtg aataatttcg acgccaaacg
     5641 ttatctcggt acctggtatg agattgcccg ttttgatcac cgttttgaac gtggactgga
     5701 aaaagtcacc gcaacataca gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg
     5761 ctataaccct gacagaggaa tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc
     5821 accaactcgc gctgcgctga aagtgtcatt ctttggtcct ttctatggcg gttataacgt
     5881 tattgcactc gatcgggaat accgccatgc gctggtttgc ggcccggacc gcgactacct
     5941 gtggatactc tcccgcacgc caaccatttc tgacgaagtg aaacaggaga tgctggcagt
     6001 cgcgacccgg gaagggtttg atgtcagtaa gtttatttgg gtacagcagc ccggtagtta
     6061 gtgagtgctg agtttcagac caataatccc caatacgatt aacgccagac tcgccagacg
     6121 catcgggtta gcggactcac cgagcagcac aatgccggtg atggccgcgc cgactgcgcc
     6181 aatacccgtc cacacggcat caaacggcta aagccgtggg tatatttcag gccaacggcc
     6241 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     6301 aatttggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     6361 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttat gacgatcgca
     6421 gaaaaaacaa acggcacgac actgctgtcg taccgttatt gcttattgct gcgctttcgt
     6481 tgctgcgcca gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt
     6541 gttgcaggca gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt
     6601 cttcaccata acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat
     6661 aatagacaac atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat
     6721 tagctggcag cgcgagagat ggag
//
LOCUS       DAJTXY010000398         3826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.guided.221, whole genome shotgun sequence.
ACCESSION   DAJTXY010000398 DAJTXY010000000
VERSION     DAJTXY010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.guided.221"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            70..285
                     /locus_tag="OO566_004432"
     CDS             70..285
                     /locus_tag="OO566_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6607412.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            278..664
                     /locus_tag="OO566_004433"
     CDS             278..664
                     /locus_tag="OO566_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR6607413.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            672..1358
                     /locus_tag="OO566_004434"
     CDS             672..1358
                     /locus_tag="OO566_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6607414.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="OO566_004435"
     CDS             complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="OO566_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6607415.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2041..3246
                     /gene="tet(B)"
                     /locus_tag="OO566_004436"
     CDS             2041..3246
                     /gene="tet(B)"
                     /locus_tag="OO566_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6607416.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3359..>3826)
                     /locus_tag="OO566_004437"
     CDS             complement(3359..>3826)
                     /locus_tag="OO566_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6607417.1"
                     /translation="ALVTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGW
                     SALESIFEHYLDYIIDNNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQE
                     NQLKAYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT         1067 a          698 c          819 g         1242 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc gaccactatt aagtcatgta
       61 gctggtttta tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg
      121 ctatcttggt gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg
      181 aaagctcaac aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat
      241 attactcgcg aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt
      301 agataatcaa ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg
      361 attggaaggg ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga
      421 acaaggaaag gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg
      481 gacaccatta attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg
      541 agtattggcg gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact
      601 gatattaata acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa
      661 gtgaacttgc gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg
      721 cgataggggc ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg
      781 ggcgagcttg gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga
      841 gcagtcattt atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc
      901 atcgttattt tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga
      961 tctccttaaa ccatggcgta catgccaaag tttccacgcc agtgcattta cgaaaagcac
     1021 gtacttgcta tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc
     1081 aacagcaatg gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc
     1141 atgaaatggg aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag
     1201 attggagtga acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt
     1261 atgaatctaa agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa
     1321 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
     1381 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     1441 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     1501 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     1561 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     1621 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     1681 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     1741 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     1801 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     1861 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
     1921 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
     1981 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     2041 atgaatagtt cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc
     2101 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
     2161 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
     2221 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
     2281 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
     2341 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
     2401 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
     2461 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
     2521 ttttttatcg ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt
     2581 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
     2641 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
     2701 caattgatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
     2761 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
     2821 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
     2881 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
     2941 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     3001 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     3061 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     3121 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     3181 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     3241 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     3301 cccaagaggg cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt
     3361 atttatcttt caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat
     3421 cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta
     3481 agtttttaag cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga
     3541 caatggcgtt taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac
     3601 caatgatagg catcacttct tggataggga taaggttttt attattatca ataatataat
     3661 caagataatg ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg
     3721 ttgtgatgta ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt
     3781 ttttattttt aaagtgatga taaaaggcac ctttggtcac caacgc
//
LOCUS       DAJTXY010000399         3813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.guided.222, whole genome shotgun sequence.
ACCESSION   DAJTXY010000399 DAJTXY010000000
VERSION     DAJTXY010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.guided.222"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            complement(<1..484)
                     /locus_tag="OO566_004438"
     CDS             complement(<1..484)
                     /locus_tag="OO566_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6607418.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     G"
     gene            complement(610..960)
                     /locus_tag="OO566_004439"
     CDS             complement(610..960)
                     /locus_tag="OO566_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6607419.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(1163..2176)
                     /gene="intI1"
                     /locus_tag="OO566_004440"
     CDS             complement(1163..2176)
                     /gene="intI1"
                     /locus_tag="OO566_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6607420.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2336..2809
                     /locus_tag="OO566_004441"
     CDS             2336..2809
                     /locus_tag="OO566_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6607421.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2902..3693
                     /gene="aadA1"
                     /locus_tag="OO566_004442"
     CDS             2902..3693
                     /gene="aadA1"
                     /locus_tag="OO566_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6607422.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          889 a         1048 c         1059 g          817 t
ORIGIN      
        1 cgccagcctc gacacgttgg cgcagttcgg caatacgctc agacgacagg gatttcttcc
       61 tgccacggta agccccgcgt tgcttggcga gcgcaatacc ctcgcgctga cgctcgcgga
      121 tcagggcgcg ctcgaactcg gcgaacgcgc ccatcaccga gagcatcagg ttcgccatcg
      181 gagagtcttc gccagtaaaa ctgaggtgtt ccttgacgaa ttcgatatgc acgccgcgtt
      241 gtgtcagcgt ttgcacgatc cggcgcaaat catcgagatt gcgcgccagg cgatccatgc
      301 tatgcaccac cacggtgtcg ccggtgcggg cgaagcttat cagcgcttcc agttgcggac
      361 gcttgacatc cttgccggat gccttgtcgc taaaagcgcg atcaaccttg acgccttcca
      421 gttgccgttc cgggttctgg tcgaaggtgc tgaccctgat atacccaatg cgctgtccag
      481 tcatggaatt ccctgcaaaa tgtcagggaa gactctatga ccttcaacga gatatgtcaa
      541 taaattcaaa attcaatcct atcctgacgc aatttacaca tggcatctga catcaggtta
      601 gggtatgcct caacctgacg gcggcgaatc acaagcgtcc ggtttgacgc tggtttcggt
      661 cgcagtgctg gcccgcgcct ggaagcgctg atagcactcc agcccgcaga aatgctccac
      721 gtactcggcc ccttccggcg tgaaggcggc atcgagcggg atttccttgc agcacacgca
      781 gcaactggtg ctcggttcat tggcgttcat ggtggtgttc ctccattggt tgacgaagcc
      841 gacgaaggcc gccgccggca tcggcctggc gaacaggaaa ccctgcaccg tgtcgcaacc
      901 cgcctgccgc aaccacgcaa ggcagtcggg tgtttccaca ccctcggcta ccacctccat
      961 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
     1021 ggggttggat ccatcaggca acgacgggct gctgccggcc atcagcggac gcagggagga
     1081 ctttccgcaa ccggccgttc gatgcggcac cgatggcctt cgcgcagggg tagtgaatcc
     1141 gccaggattg acttgcgctg ccctacctct cactagtgag gggcggcagc gcatcaagcg
     1201 gtgagcgcac tccggcaccg ccaactttca gcacatgcgt gtaaatcatc gtcgtagaga
     1261 cgtcggaatg gccgagcaga tcctgcacgg ttcgaatgtc gtaaccgctg cggagcaagg
     1321 ccgtcgcgaa cgagtggcgg agggtgtgcg gtgtggcggg cttcgtgatg cctgcttgtt
     1381 ctacggcacg tttgaaggcg cgctgaaagg tctggtcata catgtgatgg cgacgcacga
     1441 caccgctccg tggatcggtc gaatgcgtgt gctgcgcaaa aacccagaac cacggccagg
     1501 aatgcccggc gcgcggatac ttccgctcaa gggcgtcggg aagcgcaacg ccgctgcggc
     1561 cctcggcctg gtccttcagc caccatgccc gtgcacgcga cagctgctcg cgcaggctgg
     1621 gtgccaagct ctcgggtaac atcaaggccc gatccttgga gcccttgccc tcccgcacga
     1681 tgatcgtgcc gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc
     1741 gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga
     1801 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc
     1861 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca
     1921 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg
     1981 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac
     2041 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     2101 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     2161 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     2221 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2281 atgttacgca gcagggcagt cgccctaaaa caaagttaac ctctgaggaa gaattgtgaa
     2341 actatcacta atggtagcta tatcgaagaa tggagttatc gggaatggcc ctgatattcc
     2401 atggagtgcc aaaggtgaac agctcctgtt taaagctatt acctataacc aatggctgtt
     2461 ggttggacgc aagacttttg aatcaatggg agcattaccc aaccgaaagt atgcggtcgt
     2521 aacacgttca agttttacat ctgacaatga gaacgtagtg atctttccat caattaaaga
     2581 tgctttaacc aacctaaaga aaataacgga tcatgtcatt gtttcaggtg gtggggagat
     2641 atacaaaagc ctgatcgatc aagtagatac actacatata tctacaatag acatcgagcc
     2701 ggaaggtgat gtttactttc ctgaaatccc cagcaatttt aggccagttt ttacccaaga
     2761 cttcgcctct aacataaatt atagttacca aatctggcaa aagggttaac aagtggcagc
     2821 aacggattcg caaacctgtc acgccttttg taccaaaagc cgcgccaggt ttgcgatccg
     2881 ctgtgccagg cgttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     2941 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     3001 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     3061 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     3121 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     3181 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     3241 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     3301 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     3361 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     3421 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     3481 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     3541 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     3601 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg
     3661 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
     3721 cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgcggtgatg
     3781 ctgccaactt actgatttag tgtatgatgg tgt
//
LOCUS       DAJTXY010000400         2697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.guided.241, whole genome shotgun sequence.
ACCESSION   DAJTXY010000400 DAJTXY010000000
VERSION     DAJTXY010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.guided.241"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            575..1390
                     /gene="sul2"
                     /locus_tag="OO566_004443"
     CDS             575..1390
                     /gene="sul2"
                     /locus_tag="OO566_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6607423.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1477..1779
                     /locus_tag="OO566_004444"
     CDS             1477..1779
                     /locus_tag="OO566_004444"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6607424.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(1955..>2697)
                     /locus_tag="OO566_004445"
     CDS             complement(1955..>2697)
                     /locus_tag="OO566_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCR6607425.1"
                     /translation="DAVDDDPMTPLLGHSITYRIAVGSQAGRKVFTLQTLPTSGDPFG
                     DGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLTRGGNVRYQLKTPY
                     RDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALVTPAKRGRGNKVR
                     VADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIEDPIVIKQILDHL
                     KHKAETSGTRALPESRAPPAELLLGLFD"
BASE COUNT          556 a          790 c          738 g          613 t
ORIGIN      
        1 taactatcaa cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat
       61 tagtaacagg agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg
      121 gctacactga aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag
      181 cagttcccca actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta
      241 cagagcaaga gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta
      301 tcaattaatc ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa
      361 caggtttccc tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct
      421 acttattgag agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg
      481 atcaccgcaa acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga
      541 ctcatgctcg attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg
      601 catcgtcaac ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc
      661 agccattgcg caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc
      721 ggcatccagc aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc
      781 gccggtgctg gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc
      841 cgcgacgcaa gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt
      901 tccagacgct gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca
      961 ttcggtgcaa gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca
     1021 cattgcggcg ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa
     1081 ccgccttgtc cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct
     1141 ctcggtgctg gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc
     1201 tgtttcgcgc aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc
     1261 cgcgacactc gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca
     1321 cgagccgcgc cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag
     1381 aattcgttaa ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc
     1441 ctttgagggc gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca
     1501 gacggtattc gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc
     1561 ggcatggcgg ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc
     1621 aaggatacgc gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg
     1681 atgggcatgg acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc
     1741 ccttttcacc caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat
     1801 gcaacccaat ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca
     1861 ctagcggctc cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca
     1921 gcattttgat tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct
     1981 cagccggtgg cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct
     2041 tcaggtgatc aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca
     2101 tggcgccgcc gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt
     2161 gcgcccatgt catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga
     2221 ccttgttgcc cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg
     2281 cgaacacccc gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc
     2341 ttgcaatgaa atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct
     2401 tgagctggta gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg
     2461 ggcggctgat gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca
     2521 cgccggcgtg caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac
     2581 tggtcggcag agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac
     2641 ggtaagtgat cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccg
//
LOCUS       DAJTXY010000401         1043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504622
            SAMEA8900223-rid15816333.guided.296, whole genome shotgun sequence.
ACCESSION   DAJTXY010000401 DAJTXY010000000
VERSION     DAJTXY010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900223
            Sequence Read Archive: ERR6005237
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478151.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478151.1
            Genome Coverage       :: 37x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:47:41
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,446
            CDSs (total)                      :: 4,366
            Genes (coding)                    :: 3,984
            CDSs (with protein)               :: 3,984
            Genes (RNA)                       :: 80
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 197 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 132 of 382
            Pseudo Genes (multiple problems)  :: 43 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900223-rid15816333.guided.296"
                     /serovar="S. flexneri 3a"
                     /isolate="504622"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2010-06-30"
     gene            264..923
                     /locus_tag="OO566_004446"
     CDS             264..923
                     /locus_tag="OO566_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6607426.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          290 a          226 c          241 g          286 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggc
//
